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<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE29</accession>
    <name>G3AE29_SPAPN</name>
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        <submittedName>
            <fullName evidence="8">Major facilitator superfamily multidrug-resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_146938</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="EGW35563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007372913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE29"/>
    <dbReference type="GeneID" id="18870735"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_146938"/>
    <dbReference type="eggNOG" id="KOG0255">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008455_13_7_1"/>
    <dbReference type="InParanoid" id="G3AE29"/>
    <dbReference type="OMA" id="SGPMSDW"/>
    <dbReference type="OrthoDB" id="5215911at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF2">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G08910)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="313"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="377"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="441"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="468"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="72"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="498" mass="54830" checksum="AD16F4F71F6EA85F" modified="2011-11-16" version="1">MSTPSIAPSIASDMEKGTPSHVENTPIGITADLDPEHEQYLISRHGSVQLDPLPSADPQDPLNWPDWKKNYEILLIAFGSFSSTFMAAGLTPAFETMAEEYGVTLETAAYFTSAQIAVFGVLPLFWVPLMNTYGRKFFVTFGALACGVLNIGGAVSTTFGQQMATRVLVGVFVSTVTAAGSSVVGDLAFSHERGKKNGWWTLAFVVGTPSGPFFTGFIQQHAGTKWIFYVFAIMNFIQFGLWLFATETVYTRSNPVYHQSKVLKVLGIRKSESRQFHWKMFFRPLKQAANFNITLAVIAASVTFCYANIVLVVEMPQVMIPYFHLSPQQLSLQYISIIVGSFIGEVLAGPLSDWWMKRCTAKRGGNRVIADRLWISYNGYFLVIIGLIIWGVFLFQAKPGHWNIRPLIGAAIAAAGNNIVATVITTFAIDCNPSKAADVGLYINFVRSLYGFLGPFYFPYMFANLNFAGSAGLMCGLVLLFAWVPSIIAHVVGLRRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AED7</accession>
    <name>G3AED7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA-directed RNA polymerase II subunit RPB3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_58923</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373137.1">
        <property type="nucleotide sequence ID" value="XM_007373075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AED7"/>
    <dbReference type="FunCoup" id="G3AED7">
        <property type="interactions" value="1032"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AED7"/>
    <dbReference type="GeneID" id="18872681"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58923"/>
    <dbReference type="eggNOG" id="KOG1522">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038421_1_1_1"/>
    <dbReference type="InParanoid" id="G3AED7"/>
    <dbReference type="OMA" id="FYFEVES"/>
    <dbReference type="OrthoDB" id="270173at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
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        <property type="project" value="EnsemblFungi"/>
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    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07031">
        <property type="entry name" value="RNAP_II_RPB3"/>
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        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB3"/>
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        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
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    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001514">
        <property type="entry name" value="DNA-dir_RNA_pol_30-40kDasu_CS"/>
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        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
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        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001988">
        <property type="entry name" value="PRK00783.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF2">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00446">
        <property type="entry name" value="RNA_POL_D_30KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="8">
        <location>
            <begin position="21"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="302" mass="33829" checksum="284A493AFC268C3A" modified="2011-11-16" version="1">MNMDIDTSGPSVTIREAERDHVNFILKNVSLASANSIRRTMLAEVPTLAIDLVEIDVNTSVLADEFIAHRLGLIPLVSEGIENLTYSRDCTCDNYCSKCSVRLELTAKCDSDSTMSVYASDLAKFHNGSRLGDPVIRDAQGRGSLICKLRKHQELRIVCIAKKGIAKEHAKWSPCAAVGFEYDPWNKLKHTDYWYEIDADKEWPKSENCEWEEMPDPEAPFDYTAKPTNFYFDVETVGNLPPNEVVVRSIETLQRKLADIAIELNKESVEASNANAGGFTTYGRDYDGDSPMGRTPYGEWGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AES3</accession>
    <name>G3AES3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_64786</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64786"/>
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    <dbReference type="OMA" id="IDIRNIF"/>
    <dbReference type="OrthoDB" id="4016968at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="29"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="399" mass="46596" checksum="61A3544D7DCF47D9" modified="2011-11-16" version="1">MITTLFYLYFLTIIRFLFLQLDSMIAIRKPLILTLPDEIIYEILGYLNQINILSVRLINLRFYIVATEKLFNFVYVNNGDPVVIGPDVNSPFYLKYTVVNGWNKFITLLNMKELQFVNTIVIYTDYGITLDFKKVIRSSPRISIEFEYKKSIIVHQELSFSSWFRIAMTEFNYSIKKLTINGGETKLLPFFTRLKSLRIINGTSTMFDSLKQQITIRDLSLEFLQQPIRLIDIRNIFNLAEIVSLELKFGLYHPPPSVVELAILASQLKSVKQLAIISPNIRFDKVVHQLKPNSITSFYLKILGNYYETPSSGLVEILKYQQQSVMKVYWSCVRSNSRFRSYGLQEFDRVYKKSGDNNKLEEVVFLLNNGRFEKLNQVVLNENYFAIKSLRNTEIVCLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
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        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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            <taxon>Saccharomycotina</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007373110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AET0"/>
    <dbReference type="FunCoup" id="G3AET0">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="GeneID" id="18872698"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58962"/>
    <dbReference type="eggNOG" id="ENOG502QR4N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_585483_0_0_1"/>
    <dbReference type="InParanoid" id="G3AET0"/>
    <dbReference type="OrthoDB" id="289721at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053949">
        <property type="entry name" value="SBE2/SBE22_M"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22874">
        <property type="entry name" value="SBE2_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SBE2/SBE22 middle" evidence="1">
        <location>
            <begin position="9"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="467" mass="54647" checksum="B30171E19711C2AC" modified="2011-11-16" version="1">MPTLDENSSITSSTDVSFNDDYEIAQNISQFYNHRSASMSKLIKLTRQENIMYKLPTFVKSQSSMEDLHLISPEKLTYVDQTRPINLPPKTEQDKIKHNKEFKQVISDYEVNTKATNDERIRNVQTQLQFKQAWFKIIDSFSELDSKAFNKKFNYEKNNIRKLIWDCNIPSNNRFNFLTKILSTNQNSQDSLNTIKNSFKLFDQKYHNLSDQVKTSKNDEFDNIIENCLQRPLIKCISDNVDHAQFKHNYRYLLYIKSLSEFGLHKHDEVFLIPVLLILFQDESLIDIYCLLELLNQEVFDRDLISQLNSSLGQWADSRNIPRTLGKFSTSEFENLNSNHMFEMILQFSDKLPLSLSASSTPIVSQPPTFESIEDPISTSASACFELVSKLISLLVVYSSSIKTKTKNNSKLWQTFIVVLFKYYHLNWNDYQELIKENVSIRLNNSLDMQTNLDSFVEKWKGIYKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AET9</accession>
    <name>G3AET9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GYF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_53929</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373181.1">
        <property type="nucleotide sequence ID" value="XM_007373119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AET9"/>
    <dbReference type="FunCoup" id="G3AET9">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AET9"/>
    <dbReference type="GeneID" id="18871822"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53929"/>
    <dbReference type="eggNOG" id="KOG1862">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011552_0_0_1"/>
    <dbReference type="InParanoid" id="G3AET9"/>
    <dbReference type="OMA" id="THLDTEW"/>
    <dbReference type="OrthoDB" id="48509at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051640">
        <property type="entry name" value="GRB10-interact_GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003169">
        <property type="entry name" value="GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445:SF36">
        <property type="entry name" value="FI03272P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445">
        <property type="entry name" value="GRB10 INTERACTING GYF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02213">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00444">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50829">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GYF" evidence="2">
        <location>
            <begin position="170"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="117"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="386"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="404"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="424"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="485"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="512"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="870" mass="95009" checksum="B87B67A9C7DDEC0B" modified="2011-11-16" version="1">MYSRPRRNQSLDHQVNNGLDSHESPAASYTPTPTIQPNKGSKRYSMNEVFQVWYDNKDQILNPAVDLPIKASESYRLAKPSQIYHLDLQPSSFDDVHHQEQVNESLDKLSIDDYAEQASQQQSQQQQQQQQQSDLANAASHLTGPPPGISENVLSREQTNNSNPLVTSDKIEWYYIDPLGTEQGPFNGDMMQEWLTGGYLTLDLKIRRKEESTFKTLKDLCDSVQNYIQPFKVPLPDLTFIPAEPSAIDTSSTPTSQFFQNQLPDSLPSQQPQSQFHHHFLPEGNLGPGGVRLPGLSTGPQSGLFANDWKNDPFSPITPSSATATFPATAPVHGHHPHVQQHHHFGIETFNHPFGSTHPLQHSAMPSLLQPQIQQPLSGTGWGLDTSNTSTLISSSNPATPVNPVMVPQPAQVTQPTPISPWISSNVQSSSRVSSPFAPSSSLHSSNSKGPSPLLDTGKEASTGDDTVLDEIHNVVTGILDEEVAEPKDQVKEVKKVETEPDVKPEPIPEPVEPEVVAEEESPEEEEEVSQDSEEEENQGVQLKPAVPQELAPWASKPQETKPKLSLKEIQQMEAEKLERQKRIEAQIRSEQAKAWASLDEPQPAEKAVPAFPATWASAEIHREEAEAAAKAKAAAVAAAASAAAKPSTGAASVTLPVRASLASTLANAVPKDEGPAWTTVTSKKLPVVKKATPVVTNSATKTNPQLLRAVSAAKPAAPSVNANAVREDFLVWARSNMSNLYPSVTKDDLLDIFITLPSNSTDSVQLISETIYSSSATMDGRRFAQEFMKRRQKVDQTIGYAEAHDQVAWASAVISSADKVQTVDEDGWSTVGLFILAVLLKYFRGSPAQVLEPRKEAVFREDFKVAAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF16</accession>
    <name>G3AF16_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SPX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_47897</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372232.1">
        <property type="nucleotide sequence ID" value="XM_007372170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF16"/>
    <dbReference type="FunCoup" id="G3AF16">
        <property type="interactions" value="109"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF16"/>
    <dbReference type="GeneID" id="18871156"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_47897"/>
    <dbReference type="eggNOG" id="KOG1281">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005170_8_0_1"/>
    <dbReference type="InParanoid" id="G3AF16"/>
    <dbReference type="OMA" id="GYGLMYI"/>
    <dbReference type="OrthoDB" id="10260443at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990816">
        <property type="term" value="C:vacuole-mitochondrion membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015169">
        <property type="term" value="F:glycerol-3-phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006797">
        <property type="term" value="P:polyphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000185">
        <property type="term" value="P:regulation of phosphate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01115">
        <property type="entry name" value="SLC13_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14478">
        <property type="entry name" value="SPX_PHO87_PHO90_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF92">
        <property type="entry name" value="LOW-AFFINITY PHOSPHATE TRANSPORTER PHO91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="392"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="421"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="471"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="517"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="558"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="601"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="645"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="670"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="703"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="746"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="782"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="820"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="8">
        <location>
            <begin position="1"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="137"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="854" mass="94049" checksum="FB4A7D0A4B473C30" modified="2011-11-16" version="1">MKFSHSLQFNAVPEWSSKYIAYTTLKKLIYSLQRDNLRRGYEGEGTDLEAAHLITEGGYASTGRLGDTESVSAVFIAALDAELKKIDSFYQQQEEFIYKSIDQLMNDIENFEHEIDSSLVMSSQFKARGRAHSALSSDYVVNTTDPDTEFTQAEDDDDEEEEEVGTEGAASDDGSKRKRFASVPNLPSDGATSLRHSRSIDEYLKSPRSPEVWNNLNQVAGKANVPPQLLLLSESRIILRKRIIGLYTTLSELKSYIELNQTGFKKASKKFDKSLNTNIKDEYLKSLPEKSYIFKPATIDKVDDRLEALIKLYALICNQGDDLEAAKSELSIHLREHVVWERNTVWRDMIGLERKSYAANAKLSDRERGEKAEEGSDNKMIQGFNISLKNPVVLKFLTILTITVVLLNFSPFTDSAQKNCFALLICASLLWATETIPLFVTSLLIPLLIVNLQVIKNDDGTIMPTVDASKFILSTMWNSVILLLLGGFTLAAALTKYHIAKLISTWILSKAGTNPSVVLLTIMGVALFASMWVSNVAAPVLCFSLIQPLLRTLPKGSQFINALILGIALASNIGGMASPIASPQNMIAIGSMEPQPSWGQWFVIALPVCILSLLLIWIFLLITFNCNSRNTHLVAIRTIDDKFSAIQWFIIAVTVLTIFLWCIASRLTGIFGEMGIIALIPLVLFFGSGLLNTEDFNNYPWNIVILAMGGTALGKAVASSGLLNTIAIEIQHQVEDFSLFGVTLTFGFLILTMATFVSHTVAALIIIPLVSEIGNNMAEPHPRLLIMASALLCSAAMGLPTSGFPNVTAICMTDEFGKPYLTVGTFITRGVPSSVIAYAIIVSVGYATMKIINF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF74</accession>
    <name>G3AF74_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquitin thioesterase OTU</fullName>
            <ecNumber evidence="11">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_53257</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="11"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372275.1">
        <property type="nucleotide sequence ID" value="XM_007372213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF74"/>
    <dbReference type="FunCoup" id="G3AF74">
        <property type="interactions" value="717"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF74"/>
    <dbReference type="MEROPS" id="C85.006"/>
    <dbReference type="GeneID" id="18871772"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53257"/>
    <dbReference type="eggNOG" id="KOG3288">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049327_0_0_1"/>
    <dbReference type="InParanoid" id="G3AF74"/>
    <dbReference type="OMA" id="VDEYCAW"/>
    <dbReference type="OrthoDB" id="65596at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22745">
        <property type="entry name" value="OTU_OTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.80:FF:000016">
        <property type="entry name" value="Putative ubiquitin thioesterase otu1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048857">
        <property type="entry name" value="OTU1_Ubl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057766">
        <property type="entry name" value="Znf-C2H2_OTU1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13312">
        <property type="entry name" value="HIV-INDUCED PROTEIN-7-LIKE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13312:SF0">
        <property type="entry name" value="UBIQUITIN THIOESTERASE OTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21403">
        <property type="entry name" value="OTU1_UBXL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24560">
        <property type="entry name" value="zf-C2H2_OTU1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="OTU" evidence="12">
        <location>
            <begin position="120"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="338" mass="38725" checksum="DA46FAF255BD4202" modified="2011-11-16" version="1">MRLKLKSRIGTCTINIDDSKLFRDFLEDIKNNSQLHLETERISSIKSGFPPKPLDLTFSSPDVTVSDKGIKDGDQLIVEFVHSGGVKRPLSESPVPPVRKRPTPSEEIPAVYIPELDRYIILRNIPDDNSCLFNSISYAMSGYDSYRTISPPGDLRNVVVEYIEKDPELYSELVLGRPRDEYCEWIRKKDSWGGAIELGILADWFDIRIVCIDIESGNFIRFENEKKPPKRFIILIYSGIHYDLLSLNEELSQNERDMKNDISNWPIEESKQEELILASSQKLCKKLQENDYATNTTTFRVRCLDCYQILVGEMGASKHAEETGHVNFGEVKKKTPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF96</accession>
    <name>G3AF96_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">glucan 1,3-beta-glucosidase</fullName>
            <ecNumber evidence="9">3.2.1.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_58016</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1-&gt;3)-beta-D-glucans, releasing alpha-glucose.</text>
            <dbReference type="EC" id="3.2.1.58"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.58" evidence="9"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372296.1">
        <property type="nucleotide sequence ID" value="XM_007372234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF96"/>
    <dbReference type="STRING" id="619300.G3AF96"/>
    <dbReference type="GeneID" id="18872282"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58016"/>
    <dbReference type="eggNOG" id="ENOG502QPYU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004624_0_1_1"/>
    <dbReference type="InParanoid" id="G3AF96"/>
    <dbReference type="OMA" id="WVVDEWT"/>
    <dbReference type="OrthoDB" id="62120at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF1">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE I_II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442268" description="glucan 1,3-beta-glucosidase" evidence="11">
        <location>
            <begin position="20"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="12">
        <location>
            <begin position="72"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="462" mass="53213" checksum="2B5F5CB040FF69DD" modified="2011-11-16" version="1">MLLYLRLLVLTTWVVQVLANNSTAFQYKGISIGGWLVLEPYITPTLFKDSLNPGESEDDIPVDEYHYCKKLGKEEAERRLNQHWSEFYNETDFQLIKQAGLNMVRIPIGYWSFEMMDKDPYVSGAQDYLDKAIEWSKQNDLKVLIDLHGAPNTQNGFDNSGLRNIGYPGWQNKTEYVDLTVKILKQIYNKYGTGEFAEKYNDTIIGIEVLNEPYGPALSMNKLKSFYIDTYNDARNIQNFTNSIMFHDAFQGIGDWDDFLSRGKVQVVFNNGTRNITVTKSAKFNNVVLDHHHYEVFADSVHSNITTHLQNIKDYAGSIKKEKNGAIVGEWSAALTDCAMWLNGIGLGTRFEDTAPYGNKTSNGQCAKWTDAKKWSKQQKKDYRRFIEMQLYEYGINTQGWIFWCWKTESATEWDFQALVKYDIMPQPLDNYKYVKNGVDVSGSTKFGLNWVLLISMLVVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFU6</accession>
    <name>G3AFU6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58229</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372497.1">
        <property type="nucleotide sequence ID" value="XM_007372435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFU6"/>
    <dbReference type="GeneID" id="18872372"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58229"/>
    <dbReference type="eggNOG" id="ENOG502THDG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2758670_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFU6"/>
    <dbReference type="OMA" id="CAAHLIN"/>
    <dbReference type="OrthoDB" id="4079124at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008698">
        <property type="entry name" value="NDUB7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05676">
        <property type="entry name" value="NDUF_B7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="78" mass="9231" checksum="640B2094EBAE1D00" modified="2011-11-16" version="1">MTQTEQYVYPPMPSEAELDEQDVPFIHRDRCAAHLINYYKCLDKGISFCTATKEEFYKCQYIALKERLANHTKQTQTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG33</accession>
    <name>G3AG33_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_131499</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372584.1">
        <property type="nucleotide sequence ID" value="XM_007372522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG33"/>
    <dbReference type="STRING" id="619300.G3AG33"/>
    <dbReference type="GeneID" id="18869582"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131499"/>
    <dbReference type="eggNOG" id="ENOG502SWM7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_552730_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG33"/>
    <dbReference type="OrthoDB" id="46189at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="469" mass="54134" checksum="5FC718625DA73F26" modified="2011-11-16" version="1" fragment="single">MSSITMSSEDVASIDEMFIRLPIAQIKQLSHEYNVKIAQTKEDLHTLVGNKYRDLIKIAEDIDSMYSISQASDVQISNLSYKPAKFVSFTHDNYSKFDSMIRKDSAQEARLNSRAIVVRNIINKKLAKLDYKIRSGGSPLIHTSNFIYYAKVYYTIEKSFGDVLENNKSINSQFTTMKDKFKSYLESELSCYNLTDSIVRSTNNDKFRFSQRLTLKELTTNVLQDDYIDEELEEEELEEDEDKFENYELVNSKIESYDLHTLPINNYLISLTILLQTSFEEIVSSFIDIRFKFLQDLIEQVRSNSPEKINFFKIFKYIENTICYIQGYFKSGTSDYFVGLDSVRTPWKASNLTGHRSWFDDTTIRFNLEGYKPITTSVELSQFPTLVFDMINELLTQEETVSGVTNLSSTIFMFHNIMLSLAKLQDSIELSGSTSNLITMISSTDLLPNLLSQVIFKISDSFDMHISQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGD1</accession>
    <name>G3AGD1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TATA-box binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_58486</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBP family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372682.1">
        <property type="nucleotide sequence ID" value="XM_007372620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGD1"/>
    <dbReference type="FunCoup" id="G3AGD1">
        <property type="interactions" value="1136"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGD1"/>
    <dbReference type="GeneID" id="18872469"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58486"/>
    <dbReference type="eggNOG" id="KOG3302">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060161_4_2_1"/>
    <dbReference type="InParanoid" id="G3AGD1"/>
    <dbReference type="OMA" id="NCEYEPE"/>
    <dbReference type="OrthoDB" id="2127950at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000120">
        <property type="term" value="C:RNA polymerase I transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097718">
        <property type="term" value="F:disordered domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008301">
        <property type="term" value="F:DNA binding, bending"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001179">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000979">
        <property type="term" value="F:RNA polymerase II core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001092">
        <property type="term" value="F:TFIIA-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006359">
        <property type="term" value="P:regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001188">
        <property type="term" value="P:RNA polymerase I preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070898">
        <property type="term" value="P:RNA polymerase III preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04516">
        <property type="entry name" value="TBP_eukaryotes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000001">
        <property type="entry name" value="TATA-box-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000002">
        <property type="entry name" value="TATA-box-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030491">
        <property type="entry name" value="TBP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033710">
        <property type="entry name" value="TBP_eukaryotic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="22"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="27332" checksum="43F485ED8C495D49" modified="2011-11-16" version="1">MDSLKLPTTAAQAKAFTAPNSLLFPKGIDGEQQQQQQQKTPETDIVKKEETSDIIEPKQDEDDGATSGIVPTLQNIVATVNLDCRLDLKTIALHARNAEYNPKRFAAVIMRIRDPKTTALIFASGKMVVTGAKSEDDSKLASRKYARIIQKLGFNAKFTDFKIQNIVGSCDVKFPIRLEGLAFSHGTFSSYEPELFPGLIYRMVKPKIVLLIFVSGKIVLTGAKQREEIYAAFEAIYPVLSEFRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGF3</accession>
    <name>G3AGF3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Arp2/3 complex 34 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_58512</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Arp2/3 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
            <location evidence="1 6">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ARPC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372703.1">
        <property type="nucleotide sequence ID" value="XM_007372641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGF3"/>
    <dbReference type="FunCoup" id="G3AGF3">
        <property type="interactions" value="773"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGF3"/>
    <dbReference type="GeneID" id="18872477"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58512"/>
    <dbReference type="eggNOG" id="KOG2826">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059439_1_0_1"/>
    <dbReference type="InParanoid" id="G3AGF3"/>
    <dbReference type="OMA" id="FRSYFHY"/>
    <dbReference type="OrthoDB" id="148331at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072697">
        <property type="term" value="P:protein localization to cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1460.20:FF:000003">
        <property type="entry name" value="Arp2/3 complex 34 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007188">
        <property type="entry name" value="ARPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034666">
        <property type="entry name" value="ARPC2/4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12058:SF0">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12058">
        <property type="entry name" value="ARP2/3 COMPLEX 34 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04045">
        <property type="entry name" value="P34-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69645">
        <property type="entry name" value="Arp2/3 complex subunits"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="330" mass="38289" checksum="21259ECAA1120006" modified="2011-11-16" version="1">MLHIEQNNLLLQNTLTERLYPSDPSANPKSLDRIVSDFDFTTFHVSTLSDRNLILISVYLRCWDDLVNYGVVNYLSNKYANFPSLTVLTGGEIEQGYNFSIVLDVKAAIEESEESKQELIEQLSLLKRHSMAAPFEQAFARYDELSKQYANSNTYSEEVQQELLNEPILVIKYRGIDESIYLKPSFDRVTVIFSTVFQDETDKIFGKVFLQEFMDARKRAVQTAPQVIYTHGSPPLDIQQVVHGGDNDNKGYVTFVLFPRHLVKGDKRDNCISHIELFRSYFHYHIKCAKAYMHSRMRFRVKEFLKILNRAKPENVVEKKTASGRRFDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGG6</accession>
    <name>G3AGG6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58526</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372717.1">
        <property type="nucleotide sequence ID" value="XM_007372655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGG6"/>
    <dbReference type="STRING" id="619300.G3AGG6"/>
    <dbReference type="GeneID" id="18872488"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58526"/>
    <dbReference type="eggNOG" id="ENOG502T4H3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068304_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGG6"/>
    <dbReference type="OMA" id="DNMYGIK"/>
    <dbReference type="OrthoDB" id="4095418at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442042" evidence="1">
        <location>
            <begin position="19"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="344" mass="37098" checksum="5D4C7BCAC43EB3F3" modified="2011-11-16" version="1">MKFSSLITAIPIFGMVMGHTIDYDSQLQEILTPPQFEKRASNNSLVPLLQTLAGSGLIPNLLSEITSSQQKMDTVADLILSAVQGGSSIFSNISLNLNMNVSTTELLSGITGLLPSVFQGLLVNETNRNVIAGVASGLLGSKEYVWISALILELGQGADLTVDLLANLMINGTTYMKFSDYGKIEEVNEVEKRDTDDNDGSFSQLLNNMVGTLLNSNLFKGTAQQLFDAIEESGIVVPLVLDIITIPTLPKFAGVVLKKLYDNHVFDGIDLNSYYVAIKKSNLLTDSTEALLTDETFGPAVALLLKRIEDLGTFDAITRNMYGDLAKIPSESAKSSDAAAEPTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGG9</accession>
    <name>G3AGG9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_146412</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372720.1">
        <property type="nucleotide sequence ID" value="XM_007372658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGG9"/>
    <dbReference type="FunCoup" id="G3AGG9">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGG9"/>
    <dbReference type="GeneID" id="18870716"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_146412"/>
    <dbReference type="eggNOG" id="KOG2276">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008535_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGG9"/>
    <dbReference type="OMA" id="HATVCVD"/>
    <dbReference type="OrthoDB" id="7832001at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061672">
        <property type="term" value="C:glutathione hydrolase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051458">
        <property type="entry name" value="Cyt/Met_Dipeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017149">
        <property type="entry name" value="GSH_degradosome_Dug2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270">
        <property type="entry name" value="BETA-ALA-HIS DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270:SF8">
        <property type="entry name" value="DI- AND TRIPEPTIDASE DUG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037237">
        <property type="entry name" value="Peptidase_WD_repeats_DUG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="5">
        <location>
            <begin position="675"/>
            <end position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="924" mass="103679" checksum="212085780DEAFD95" modified="2011-11-16" version="1">MYHNFTNKIIHEGETVIPKRQTLPINSTSSGDNLVATPPKAATPLRLNSPGDTSFHDMPKLIHKWTRTLSILCCVASPVHKLLFCGTQDGKILVYDLSDYSLTKEIDAGGQQHQPPSILTMCLSKDENYLFVAGSDSLIKVFDLSGNDFHCTHIIYSLVDIGDIFSIAWCDESSTVYIGAQNASIVWCKLSFDKSSFTSRSNIERLPSFRYDKFFDSKGPGGSQNTLQTKHEIYRKNSSSTTRNSPHTKHLKLVEAGSEDIIRFSHNGYVYCMDIVNAEIGDFHERKYLISGGGDGSINIWSIDPSNGGTPVKLTSLANEESVLSMSVQGSLLYVGLGNSCVNVWDLTTLQLIRSFHFSAELREHPHLQRNHNLHQKQHQDNEVLSLAIYNDSIFKACSHSGLIKLAMKSKNVSLHDSILMSVEEEDGIINTVTVFTIDSVTYLLSGGNKSLCLWDITRSTPIQEDRTPPVISLADKLSNDNLLSTLKKYISYRTISKNPQLYLEDSRHCAQFLSRLLVDLGAYQTRLLPVHNGNPIVFSVFTKNDSSVSQSELNKKVRLLWYAHYDVVDATNHEAEDWATDPFTMTARDGNLYARGVSDNKGPTLAAIYSVAELYQKQELSCDVVFIIEGEEECGSFGFQQAIHEHRSMIGDIDWIVLSNSYWLDDNTPCLNYGLRGVINASVIIKSDKPDRHSGVDGGVSKEPTMDMMHVLGQLMDPVTSQIQLDHFYDDILPMSQTESGLYEKIHDVIHTTSSTLIRKWREPSLTIHKIQVSGPNNNTVIPQVANATISIRIVPNQDLATIKQQLINKLQSSFDRLQSENSIQINIFHEAEPWLGDPTNKVYSLLYEKMQTHWQQEPLFIREGGSIPSIRFLEKCFNAPAAQIPCGQASDNAHLNDEKLRIINLFKLRLILTDTFKELGRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGY0</accession>
    <name>G3AGY0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_53094</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372065.1">
        <property type="nucleotide sequence ID" value="XM_007372003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGY0"/>
    <dbReference type="FunCoup" id="G3AGY0">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGY0"/>
    <dbReference type="GeneID" id="18871756"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53094"/>
    <dbReference type="eggNOG" id="KOG3798">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020884_2_1_1"/>
    <dbReference type="InParanoid" id="G3AGY0"/>
    <dbReference type="OMA" id="CTPAMHW"/>
    <dbReference type="OrthoDB" id="332863at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070290">
        <property type="term" value="F:N-acylphosphatidylethanolamine-specific phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070291">
        <property type="term" value="P:N-acylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070292">
        <property type="term" value="P:N-acylphosphatidylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024884">
        <property type="entry name" value="NAPE-PLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15032">
        <property type="entry name" value="N-ACYL-PHOSPHATIDYLETHANOLAMINE-HYDROLYZING PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15032:SF4">
        <property type="entry name" value="N-ACYL-PHOSPHATIDYLETHANOLAMINE-HYDROLYZING PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038896">
        <property type="entry name" value="NAPE-PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="201"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="465" mass="53933" checksum="97E8F5B80A04D5A7" modified="2011-11-16" version="1">MGMSFVSRLSLKARVGWGLLLGYTSFEVYLHFRTQAIINSRKQEYNKVIREQATDDIDVSKFKSSLVAGMFINPFEEYRNQTVFEFLLVRILELLESVYGSKIEIHQKLPHPHDGAVEVEDILKVFQPDLERFRKNSIILSQCLSKNDYSDLSVRPIDPQHIQRSWFHTKSVVPPGPPIHDQLLFTWLGQSCTLVQLAGVNFLTDPIISDHLFSPKFGPKRLTKSPMTIDSIKYATNDKLDFVIVSHDHPDHLEYDFAKHIGNNSMWIVPLGLKRKLGRKGIYKVIEMDWWEKLDITRYISTDENFPDKYEIECLPSMHWSGRYAFDSNWSLWCSYAIRRNGECILYHTGDTGYSKELFDVIGKRLGSVTLGLLPIGQYCPSWHQKPRHISPEECLKICSQLNIKYMKGIHWGTYKLSSEPILEPMTKLEKLAHKQGKAIQYRVPEFGLTYLFDLNNKTETEIHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH51</accession>
    <name>G3AH51_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH-ubiquinone oxidoreductase 9.5 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58726</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372894.1">
        <property type="nucleotide sequence ID" value="XM_007372832.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH51"/>
    <dbReference type="STRING" id="619300.G3AH51"/>
    <dbReference type="GeneID" id="18872573"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58726"/>
    <dbReference type="eggNOG" id="ENOG502S74C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166990_1_0_1"/>
    <dbReference type="InParanoid" id="G3AH51"/>
    <dbReference type="OMA" id="EKPAIFW"/>
    <dbReference type="OrthoDB" id="416470at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22903">
        <property type="entry name" value="NI9M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039961">
        <property type="entry name" value="Nuo9.5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38488">
        <property type="entry name" value="OXIDOREDUCTASE 9.5 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G08980)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38488:SF1">
        <property type="entry name" value="OXIDOREDUCTASE 9.5 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G08980)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="83" mass="9850" checksum="C7AB809E12F89271" modified="2011-11-16" version="1">MAAHQKVYGDYPVYYKNPIRWARYHAHTKPHFVFAVFFAVSAPVLLLLTPLRRKFLYDDHTPLPYVYPLPNRPRDKNLSGYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHN8</accession>
    <name>G3AHN8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_54374</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373786.1">
        <property type="nucleotide sequence ID" value="XM_007373724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHN8"/>
    <dbReference type="GeneID" id="18871858"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54374"/>
    <dbReference type="eggNOG" id="ENOG502RQ8V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_903139_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHN8"/>
    <dbReference type="OMA" id="FEEEATN"/>
    <dbReference type="OrthoDB" id="3993307at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="68"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="105"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="344" mass="39574" checksum="4B7B88AEE2A0D6F8" modified="2011-11-16" version="1">MSVVQQGQVTPSTPSKRKSFSILTPISPNRMLQHQRSLLKSPEKRERKISESTGLAKFSLANSPAKKDFFNKSESNTNTTPRSGLPVYVPIRSPPSELNTPPRTMDDLQTESRRLLEQYTTKQAQLHEYERLVEVKKMQLLELTQKIEAIKREEVILSKHGNGDIYKQAHLETERELNKLNIVSTAENNDKENNRPSLTKQVSQVFEVKTSTIKKQASFIMNQAFDDLNHNVKKSTSTIFSPQRNEDFKQLIQQSNVKFDDLAKQTSKFFNDVTSNFNDNIFNFNKNKSPSKISESFVADSSFIFDNLKESLQDEIDTNSIIYERSFEDSQEHIDIDDYDSSFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHP2</accession>
    <name>G3AHP2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Alpha-1,2-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_70356</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373790.1">
        <property type="nucleotide sequence ID" value="XM_007373728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHP2"/>
    <dbReference type="FunCoup" id="G3AHP2">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHP2"/>
    <dbReference type="GeneID" id="18875320"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70356"/>
    <dbReference type="eggNOG" id="ENOG502QQ16">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013298_1_0_1"/>
    <dbReference type="InParanoid" id="G3AHP2"/>
    <dbReference type="OMA" id="KGYQYKA"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646:SF1">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="76"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="82"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW34206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="617" mass="71321" checksum="11967D41800DFD9A" modified="2011-11-16" version="1">MVNNFLYNLKLFRRRRKQQLIILSIIFIYLIFIVYRQGGDDASTSSIIDESGFKPSWWDSIVSSVKKPVKFSDIIAGNSPDNSRKDSSIEDQEATKEHEAKDDYNKQHPSEAVRLKRGYTFFNSILQVLYNGRPEVKKLDRYRTDDRIYHARYDSSAKDEQVFSEEYLSSFLQLTTQELADMKKSHQYVVDNLPGKAPNQLYHGNGIVYVGGGQFNWLTLLSIKTLRSIGCTLPIEVFIPKVDEFELELCGKVLPTMNARCIYMRNHLVSPLDKTGNNYASKFNFKGYQYKAMAILLSSFENVLLLDSDNIPVHVPDNLFTEEPFTSKGLIVWPDFWKRATSPHYYEIVGSEISETELLPKYDESIGFYRSRPQEITGANLNDIPLHERKGAIPDPSSESGQLMISKRTHFKPLLLALYYNLYGPTHFYPLFSQGSDGEGDKETFLAATVTLRHPYYQVSKFLNALGHFQNGDFVGCGMGQYDPVEDYYTGELRKELSKITDQKVLATVKEKNNRFLTSGPRMLFVHANFPKLNPWKLRIERKIYNSNGERIRLYGTGMKRITGYDFEWVQWNNMKQLLCVNNFKLEAFKDVNKEDLCREITQQLNYLKDTIKDLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHR3</accession>
    <name>G3AHR3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_59658</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373811.1">
        <property type="nucleotide sequence ID" value="XM_007373749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHR3"/>
    <dbReference type="GeneID" id="18873010"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59658"/>
    <dbReference type="HOGENOM" id="CLU_2851180_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHR3"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="65" mass="6827" checksum="9A1C54B05A16A6BA" modified="2011-11-16" version="1">MFSHIAIASHTTFVDGVGGFYQFASESASNQIATDTATEPQKNGAGKVNARNSGFMMVLAFIGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHS6</accession>
    <name>G3AHS6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_59668</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NFYB/HAP3 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373824.1">
        <property type="nucleotide sequence ID" value="XM_007373762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHS6"/>
    <dbReference type="STRING" id="619300.G3AHS6"/>
    <dbReference type="GeneID" id="18873015"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59668"/>
    <dbReference type="eggNOG" id="KOG0869">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066247_5_1_1"/>
    <dbReference type="InParanoid" id="G3AHS6"/>
    <dbReference type="OrthoDB" id="386949at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22907">
        <property type="entry name" value="HFD_NFYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000099">
        <property type="entry name" value="nuclear transcription factor Y subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027113">
        <property type="entry name" value="Transc_fact_NFYB/HAP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003956">
        <property type="entry name" value="Transcrpt_fac_NFYB/HAP3_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064">
        <property type="entry name" value="CCAAT-BINDING TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064:SF9">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00615">
        <property type="entry name" value="CCAATSUBUNTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00685">
        <property type="entry name" value="NFYB_HAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="6">
        <location>
            <begin position="15"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="152"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="155"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="167"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="282" mass="31894" checksum="6AEB4DFFB713D782" modified="2011-11-16" version="1">MEPKLQDIELREQDRWLPIANVARLMKNTLPNTAKVSKDAKECMQECVSEFISFVTSEASDKCLREKRKTINGEDILYSMHDLGFENYAEVLKIYLAKYREQQALRQERGETRATKRQLKQQAAAAAAAAAATANGEDGTAEQAVAVAIGHENEASSSTGSVSASAEYDEAIKEEEDEDVPYLNGSIDENEHHESLSHSPIGSIHSEHLYNDYEKQQIKEEERIDEEQEEHPHTKEYGYDDFINTDEVNHHHHNIEELAKQLTHENGDIDSLGITNGSNEYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHT2</accession>
    <name>G3AHT2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribulokinase/uridine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_49304</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373830.1">
        <property type="nucleotide sequence ID" value="XM_007373768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHT2"/>
    <dbReference type="FunCoup" id="G3AHT2">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHT2"/>
    <dbReference type="GeneID" id="18871326"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49304"/>
    <dbReference type="eggNOG" id="KOG3308">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058668_1_1_1"/>
    <dbReference type="InParanoid" id="G3AHT2"/>
    <dbReference type="OMA" id="FMSIPYE"/>
    <dbReference type="OrthoDB" id="10041966at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050262">
        <property type="term" value="F:ribosylnicotinamide kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034355">
        <property type="term" value="P:NAD+ biosynthetic process via the salvage pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046495">
        <property type="term" value="P:nicotinamide riboside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02024">
        <property type="entry name" value="NRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="239" mass="27705" checksum="F3D088B343B561E9" modified="2011-11-16" version="1">MTIPHDKEVVLIACGGPSSSGKTTVSKALQYLIGDSVLIHLDDFYFHDADIPIDPKTGLQNWDCPEALDFARFVKCLHDIKQKHILPKIHSLEPDADLKLSKLEERIFQKMITSSSQKFAGKKVVIVDGFMLFHDPEIFNLFDIKLFFHAPYETLKQRRESRSYQTLDSVWVDPPRYFDDIVWPAYKDSHKYLFEQSDISLSLKQDVTEKYNIFDINNDDETRLFTLIEWALNSILSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHW0</accession>
    <name>G3AHW0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein transport protein SEC22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_59695</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373858.1">
        <property type="nucleotide sequence ID" value="XM_007373796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHW0"/>
    <dbReference type="FunCoup" id="G3AHW0">
        <property type="interactions" value="1066"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHW0"/>
    <dbReference type="GeneID" id="18873034"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59695"/>
    <dbReference type="eggNOG" id="KOG0862">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054453_4_0_1"/>
    <dbReference type="InParanoid" id="G3AHW0"/>
    <dbReference type="OMA" id="FIYWRFF"/>
    <dbReference type="OrthoDB" id="1719357at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000115">
        <property type="entry name" value="Protein transport protein SEC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044565">
        <property type="entry name" value="Sec22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45837">
        <property type="entry name" value="VESICLE-TRAFFICKING PROTEIN SEC22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="15">
        <location>
            <begin position="6"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="16">
        <location>
            <begin position="132"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW34274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="214" mass="24768" checksum="380C3401A836B09C" modified="2011-11-16" version="1">MIQSTLIYRYDALPLCGSVDDDNTPGLMDQKKKCKLLITRITPNSEPQATIESGEFNIHYLIVSSIIYICICERTYPRKLAFSYLTEISNEFRNSHGQEALAATARPFGFSSFDNFLHKTKKIYQDQRAQSNLDKLNTELADVKKVMTKNIEDLLYRGDSLDKMSDLSSSLKSDSLKYRKRAQRINFEAMLRQYIPVVGVSLVFLFIIWYMFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHW6</accession>
    <name>G3AHW6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">candidapepsin</fullName>
            <ecNumber evidence="4">3.4.23.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_65435</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential cleavage at the carboxyl of hydrophobic amino acids, but fails to cleave 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17 and 24-Phe-|-Phe-25 of insulin B chain. Activates trypsinogen, and degrades keratin.</text>
            <dbReference type="EC" id="3.4.23.24"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.24" evidence="4"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373864.1">
        <property type="nucleotide sequence ID" value="XM_007373802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHW6"/>
    <dbReference type="MEROPS" id="A01.085"/>
    <dbReference type="GeneID" id="18875043"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65435"/>
    <dbReference type="eggNOG" id="KOG1339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013253_9_1_1"/>
    <dbReference type="InParanoid" id="G3AHW6"/>
    <dbReference type="OMA" id="CYNDACA"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05474">
        <property type="entry name" value="SAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033876">
        <property type="entry name" value="SAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 13" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 14" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442105" description="candidapepsin" evidence="14">
        <location>
            <begin position="18"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="15">
        <location>
            <begin position="56"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW34280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="459" mass="50785" checksum="5F9C5E24720AF97A" modified="2011-11-16" version="1">MFYQLLILYYLLAIVLTSPLKLDIEVHRRDEIILPHPYLRKRDEFRVPVSSEYSVYTTILRIGSNRDPVRVILDTGSSDLWVMSSNVSCVSNEQSKEQKTGFFDSILTALNLKATRVCTDNGAFDYLNSATFTRNDTNPFSIGYIDGVTTKGFWGYDDITIGGNPISNLSFAVAEETSSKIGVLGIGLPEAVSTNTKYDKSYENLPLKMKNLGLINRTSYSVYMNSNEARYGAILFGAIDHAKYSGNLTTLDIVNPPSSLQRLRIPISSIQIANGTNSPTTAVSKGPSALLDTGCTYSRFSKDVVDSIAKQVGATFSKDKNQYIIDCNIPATTLLRLNFNGQPIDIPMTEMIRSAGTNTCTLGIDVDESNSLSIVGDNILRRMYVYFDLEGKQISVAQAVYTDEQNIEIISDDNFQTDGQEAGSELVQEEEKNTSSASNILFSWSFLISALSVFFIYNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHZ4</accession>
    <name>G3AHZ4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Coatomer subunit zeta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_59729</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. The zeta subunit may be involved in regulating the coat assembly and, hence, the rate of biosynthetic protein transport due to its association-dissociation properties with the coatomer complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Golgi apparatus membrane</location>
            <topology evidence="1 12">Peripheral membrane protein</topology>
            <orientation evidence="1 12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle</location>
            <location evidence="12">COPI-coated vesicle membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373892.1">
        <property type="nucleotide sequence ID" value="XM_007373830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHZ4"/>
    <dbReference type="FunCoup" id="G3AHZ4">
        <property type="interactions" value="503"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHZ4"/>
    <dbReference type="GeneID" id="18873050"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59729"/>
    <dbReference type="eggNOG" id="KOG3343">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086803_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHZ4"/>
    <dbReference type="OMA" id="NELMLHS"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000013">
        <property type="entry name" value="Coatomer subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039652">
        <property type="entry name" value="Coatomer_zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11043:SF0">
        <property type="entry name" value="COATOMER SUBUNIT ZETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11043">
        <property type="entry name" value="ZETA-COAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="13">
        <location>
            <begin position="9"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="192" mass="21660" checksum="263B4D5D59189BFA" modified="2011-11-16" version="1">MSLNISLYTISAVLILDNEGNRVFSKYYHSSLEETSDSQFETSSQQIKFEKSLFGKINKINQDILLYDNHLIVYKQTNDVIVVLVAKINENESLIYSTLANLFEAISILLDNTIDKQTILDKFDMVSLAIDETIDDGIIIEYDPATIVSRVTNPPSSHYDVNLKNIDISEKGIFNALSFASKKIGERLQQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI28</accession>
    <name>G3AI28_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">RNA polymerase II subunit A C-terminal domain phosphatase SSU72</fullName>
            <shortName evidence="12">CTD phosphatase SSU72</shortName>
            <ecNumber evidence="4 12">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_59752</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. SSU72 is required for 3'-end formation of snoRNAs.</text>
    </comment>
    <comment type="function">
        <text evidence="1 12">Processively dephosphorylates Ser-5 of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the cleavage and polyadenylation factor (CPF) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the SSU72 phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4 12"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373926.1">
        <property type="nucleotide sequence ID" value="XM_007373864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI28"/>
    <dbReference type="FunCoup" id="G3AI28">
        <property type="interactions" value="961"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI28"/>
    <dbReference type="GeneID" id="18873065"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59752"/>
    <dbReference type="eggNOG" id="KOG2424">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062463_0_1_1"/>
    <dbReference type="InParanoid" id="G3AI28"/>
    <dbReference type="OMA" id="TQPNVYQ"/>
    <dbReference type="OrthoDB" id="57957at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180007">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032215">
        <property type="term" value="P:positive regulation of telomere maintenance via semi-conservative replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090052">
        <property type="term" value="P:regulation of pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902801">
        <property type="term" value="P:regulation of siRNA-independent facultative heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009302">
        <property type="term" value="P:sno(s)RNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030847">
        <property type="term" value="P:termination of RNA polymerase II transcription, exosome-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030846">
        <property type="term" value="P:termination of RNA polymerase II transcription, poly(A)-coupled"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001174">
        <property type="term" value="P:transcriptional start site selection at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000039">
        <property type="entry name" value="RNA polymerase II subunit A C-terminal domain phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006811">
        <property type="entry name" value="RNA_pol_II_suA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20383">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT A C-TERMINAL DOMAIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04722">
        <property type="entry name" value="Ssu72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU369031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="221" mass="25449" checksum="9D4FFE61A34CAB83" modified="2011-11-16" version="1">MLEDSLKICTVCASNNNRSMEAHKQLQDAGFDVKSFGTGSAVRLPGPSIDKPNVYDFGTPYQVIYDDLMSQKCRKMYESTGLIHMLDRNRQVKTAPEKWHSNSYAGKFDLVITCEERCFDSVLDDLMYRMYANDDDDANDVKKIVHVINVDIKDDNENAIIGGRGILKLVNMIHDFKRKKQEEKDYEDESDVILEDQMMAILAEWQKDHTHLPTIYGVSYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI81</accession>
    <name>G3AI81_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_54537</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373979.1">
        <property type="nucleotide sequence ID" value="XM_007373917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI81"/>
    <dbReference type="GeneID" id="18871873"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54537"/>
    <dbReference type="eggNOG" id="ENOG502RPME">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_617014_0_0_1"/>
    <dbReference type="InParanoid" id="G3AI81"/>
    <dbReference type="OMA" id="PRHTGYD"/>
    <dbReference type="OrthoDB" id="4019224at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="380"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="444" mass="48460" checksum="0042C777DDE42F98" modified="2011-11-16" version="1">MSASKPKTPVSSPKRNSAFKPSTPSPPGSDSSSGRGEKRSSRMGSANFLRTPEQNAHNTHLPFTPSVKRSNSGTLKSPDYKLNYHNVASPYSAANALKTPRHSGYDSDDNEDKRRLKLQKTPQYFGTAKKLFDNDDASPKREDLTEISSQLKHKLSSALGKIQKEDNNSNAGLSNKISFTELSFDPSKSPTKQSTNSFSPGSSFQRANLNLQTLQSSPIPKSVAQFGQDVNSPLKQSPRFSNDHPPLVDMPSPDEELSAHRALLAALSRQTRRKSIGSTTRHPRRQPSTSGGFSEDMPSFTPSHRRTVSSDVQLPPLKVALHSHDDFQPTGFKFGGGPQQANDEKTNEQDAVLSLMSLSSPQSIKFSHSRTQSLTNNNTVSSPISSRSSSVHIRSPSYGQQPIPPALPPFTRLAANREKRSPREAGNEDSDATDVEIGDLTDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AID6</accession>
    <name>G3AID6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dolichyl-phosphate beta-glucosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.117</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_59839</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-alpha-D-glucose = a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate + UDP</text>
            <dbReference type="Rhea" id="RHEA:15401"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.117"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15402"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.117" evidence="4"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373989.1">
        <property type="nucleotide sequence ID" value="XM_007373927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AID6"/>
    <dbReference type="FunCoup" id="G3AID6">
        <property type="interactions" value="664"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AID6"/>
    <dbReference type="GeneID" id="18873098"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59839"/>
    <dbReference type="eggNOG" id="KOG2977">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_9_1_1"/>
    <dbReference type="InParanoid" id="G3AID6"/>
    <dbReference type="OMA" id="HMVNTDA"/>
    <dbReference type="OrthoDB" id="3784at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004581">
        <property type="term" value="F:dolichyl-phosphate beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04188">
        <property type="entry name" value="DPG_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035518">
        <property type="entry name" value="DPG_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="14">
        <location>
            <begin position="69"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW34405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="324" mass="36650" checksum="FE116F0FC4111965" modified="2011-11-16" version="1">MNPIYVGISVIVLGFVAVYSIVVLLSHKPRLPYTSEFKYKTNDETGVRYDLPPRIDSTSEFKDSGIDLSIVIPCYNETKRLGKMLDEAIKYLEENHKNKYEILIIDDGSTDGTDEFAIEKAREYKLTPHIMRVVHLAENRGKGGGVTHGLLHSRGKYSLFADADGATKFSDISKLVEFVASTNEPALAIGSRAHMVNTDAVIKRSVIRNFLMYGLHTLVFVFGIRDVKDTQCGFKIFNFEAVKRIYPHMHTERWIFDVEVLLLAEIQKMKLKEQAVNWHEVDGSKVDLARDSIAMAIDLVVTRLAYLLGVYKLDECGKLSKKVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIF4</accession>
    <name>G3AIF4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_149277</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374008.1">
        <property type="nucleotide sequence ID" value="XM_007373946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIF4"/>
    <dbReference type="FunCoup" id="G3AIF4">
        <property type="interactions" value="114"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIF4"/>
    <dbReference type="GeneID" id="18870818"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_149277"/>
    <dbReference type="eggNOG" id="ENOG502QR4T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010150_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIF4"/>
    <dbReference type="OMA" id="LVWCVHE"/>
    <dbReference type="OrthoDB" id="3163292at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001216">
        <property type="term" value="F:DNA-binding transcription activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.10:FF:000003">
        <property type="entry name" value="C6 transcription factor (Leu3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051089">
        <property type="entry name" value="prtT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845:SF6">
        <property type="entry name" value="TRANSCRIPTION FACTOR SEF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="10">
        <location>
            <begin position="82"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="785"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="796"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="903" mass="101711" checksum="B9004EC4DAA90662" modified="2011-11-16" version="1">MKAKMEKAKVKSILPKPSPLVSTSTPPPLLPLASASGTTTNKRKSSSTAAERKKQRKSAPDSEDTSSKSKSSKQTGHRPVTSCTFCRQHKIKCNASDNYPNPCQRCQRMGLKCEIDPQFRPKKGSQIQSLKSDVDELKAKIEMLTKNESLLTQALTQHNLNYMQQQQSQQQSGVQSVNATPITDSSGSPQTMYQGNVVFSLPSITTISQPQRPLAPENTDNYSPSTVHTNSPVDHYNLPASDSANTINYPDYKPISEFILGDVKLPLAKANELHDRFVSRYLQFMPVITSRSATELYHKSQLLFWTVILISSLGEPDSTLYMSLASLIKQLAIETCWIKTPRSTHVIQALILLSIWPLPNEKVLDDCSYRFIGLAKNLSLQLGLHRGGEFIQEFSRNQVSLGPDAEKWRTRSWLAVFFCEQFWSSLLGLPPSINTTDYLLENARVDKSLPKNFRFLISLSIFQCKLVNVMGISVTRPDGLLEPSNRAGSLNMLDRELERLRFKLQFAEGSAIEVYYLYIKLMICCFAFLPGTPIEDQVKYVGSAYLASTRIITIVSNMTNESSLIELPIYVRQAMNYSVLLLFKLHLSRYLIDKYLDSSRQSIVTVHRLLRNTLSSWKDLQNDMTRTAKVLENLNIVLYKYPDVFINENEDDSSIITRMRSHLTASLFYDLVWCVHEGRRRTLLDKSSGGTVPATPKATTDRKYLPLPLPFYNQISKDDFTTKKTTSPNGTTITTLVPTDTAMAQAREQAASMNKPIEINGIPLSMLEATGSSRDTAFVRPEQEIEDRNAPQKSDQPQQQQQQQQQQQQLPLPVPAPQPHQQQLRQPKLESHGMLMPLASKSATPVNSQFLSQSPAPGLTVNTPLDQFDNFFQQQSNGWLNNDNYQDNDDFLGWFDVNSLAEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIV5</accession>
    <name>G3AIV5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_133450</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373350.1">
        <property type="nucleotide sequence ID" value="XM_007373288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIV5"/>
    <dbReference type="GeneID" id="18869749"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133450"/>
    <dbReference type="eggNOG" id="ENOG502QTY2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009538_1_0_1"/>
    <dbReference type="InParanoid" id="G3AIV5"/>
    <dbReference type="OMA" id="RQDFGNT"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454:SF69">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 11-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="534"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="629"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="629"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW33766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="688" mass="77942" checksum="093A887E59018ECE" modified="2011-11-16" version="1">MGTFSYNVNSIEGDVYIQRLSTYIRKNEEALANGLLCFSKNRNTGLKVQPLRLSFTMHHLYYLNERIDSSPLDVDVGPLNVKLDNPNQEPTFISFLANNARTSKHFDSDTRSITSMNSVKSIVSNASVYWRSFAFSKDPKVINKDIKYLYSSFTKVPCLILSPKTHINSISSYEEYPCDTSVPVKMFKNLQVLEINDYEPNEIFGWHILSEQLRILIIRKSKVSNIGEVLFNLVIDDESGRTSFNSYRQNGRRLTEVGNNSHFSSADDSFSEIAEYPQFQNNAFRYSKPEIANGGSHWAGAGSLPKDLEFHDNYTGNKDYEILPDSKWACLKQLTISETSISRVPAYTLKPFSNLVKLNLSNNLLEDLPEGLDQLVSIKYLNFADNYITSLKKLPKNLSQLLTLNMNNNKLTDLTGLENLLSLEKIDLRRNKLSEIKQLKPVVSLFMKHPDKFNSVYLSQNKLPKNYRGELFNLFNGIKYKNSMKIDDSRPGYFESALLLDTDAAIKNLEKFLHETSNRRRSVLTVSVDLDAEFNNSGNKTGEEEEEEEEQGTPNTTVVLKHKGEKLSPKKDTLLDSFSTLKIVKEVDEPPPVPSKQFELSHAALSNLLHSTPTSPLQGNHNISNQYRSSSQYATKSSKPEVNSIPRQPSNLPPTMKRSNTLPQLDRESSNIAPNIVTQIQVTAHMTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIY3</accession>
    <name>G3AIY3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Vitamin B6 transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_147865</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373378.1">
        <property type="nucleotide sequence ID" value="XM_007373316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIY3"/>
    <dbReference type="GeneID" id="18870767"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_147865"/>
    <dbReference type="eggNOG" id="ENOG502QR29">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026016_2_1_1"/>
    <dbReference type="InParanoid" id="G3AIY3"/>
    <dbReference type="OMA" id="GRYFMGY"/>
    <dbReference type="OrthoDB" id="5428495at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026030">
        <property type="entry name" value="Pur-cyt_permease_Fcy2/21/22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806">
        <property type="entry name" value="PURINE-CYTOSINE PERMEASE FCY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806:SF17">
        <property type="entry name" value="VITAMIN B6 TRANSPORTER TPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002744">
        <property type="entry name" value="Pur-cyt_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="62"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="248"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="283"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="409"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="461"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="502"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR002744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="528" mass="58406" checksum="21D979B2569DE999" modified="2011-11-16" version="1">MSDIEKNPTFSDTTSVSTLSTPRGPKYITWIYKLDSFGVETRGIERVSGEERQRIAQSTSKYVQFGQVIGLWIAACGGLTSMSSYFLPTLLLGLNLRDALVSGLISMIIGCLIPAYCSTMGPKSGCRQMVTARFLFGQWGVKFVALIAIVGGIGWSVVNCVLGGQMLVAINGNISLAVGIVVISIVSLIVAIFGIRVLLKFQTILAIPIIIACILFYVVVCQKMDYIHDSNEMIKQAGYSALTTRGNWLSFFAIGYSITATWGSGASDYYILFPENTPSYQIFLVTFFGISIPSTFVAIVGTLCGTIALSYKPWNDAYNTLGVGGLIVETFSPWGRFGKFVAVILYISLICNNIMNTYSIAFEFQLIGQYLLYIPRWVWAFIVTAIYIVLSLAGKEHFSTILSNFLPMLGYWISMYIVLLLEENLIFRSTITSRKMHESEYSGGNYDMLYNWNNWNKPKGLTRGVACALAFACGVAGAVIGMNQVYYQGPLAKKIGDYGGDLGMWLSFGFTGITYPVFRYFELKQFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ47</accession>
    <name>G3AJ47_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Hexaprenyl pyrophosphate synthetase mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_59987</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374143.1">
        <property type="nucleotide sequence ID" value="XM_007374081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ47"/>
    <dbReference type="FunCoup" id="G3AJ47">
        <property type="interactions" value="562"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ47"/>
    <dbReference type="GeneID" id="18873166"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59987"/>
    <dbReference type="eggNOG" id="KOG0776">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_1_0_1"/>
    <dbReference type="InParanoid" id="G3AJ47"/>
    <dbReference type="OMA" id="AFDYYLH"/>
    <dbReference type="OrthoDB" id="9927103at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032478">
        <property type="term" value="C:heterotetrameric polyprenyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097269">
        <property type="term" value="F:all-trans-decaprenyl-diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000010">
        <property type="term" value="F:heptaprenyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF69">
        <property type="entry name" value="ALL TRANS-POLYPRENYL-DIPHOSPHATE SYNTHASE PDSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="481" mass="53216" checksum="E89B8C8D8F5C4AEE" modified="2011-11-16" version="1">MLSIRRRILKNSVSLLRCSSTRIVTPPNTFTTAVKTAEKLVQPQSSKIFDDPFSIVSHEMSNLAKSIANLIGSGHPILNRVSSYYFEAEGKNVRPLIVLLLSKALSNIPVEQRNRIEIDTHDVTDQYHFKGTPGSKETIAGKSVDDSISPLAILHGINPKVILDPLSKPMDKLPQFDAMNGILPKQRRLAEIVEMIHTASLLHDDVIDLSDSRRGRPSGNIAFTNKMAVLAGDFLLGRASVAIARLRNPEVIELLSTTIANLVEGEFMQLKNTVMSQSDTIVNDGEKKTIPEPTGKVPTKQHEYSISRPEDVDHITNVNAAFEYYLHKTYLKTASLMSKSSRAAAVLSGSQDDIIENCYEFGRNLGLCFQIVDDMLDYTSSDAAFGKPSQADLKLGLATAPILFAWKEEPKLGELIARKFKEPGDVEIARRAVEKYGGLDQTRLMAEDYCHKALTNLRCLPESDARSALELLTNSVLTRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJP7</accession>
    <name>G3AJP7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dihydrolipoyl dehydrogenase</fullName>
            <ecNumber evidence="11">1.8.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_59335</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + NAD(+) = N(6)-[(R)-lipoyl]-L-lysyl-[protein] + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15045"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="1.8.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="9 11">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="11">The active site is a redox-active disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="11"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373532.1">
        <property type="nucleotide sequence ID" value="XM_007373470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJP7"/>
    <dbReference type="FunCoup" id="G3AJP7">
        <property type="interactions" value="1114"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJP7"/>
    <dbReference type="GeneID" id="18872874"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59335"/>
    <dbReference type="eggNOG" id="KOG1335">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_0_1_1"/>
    <dbReference type="InParanoid" id="G3AJP7"/>
    <dbReference type="OMA" id="CAQLGMK"/>
    <dbReference type="OrthoDB" id="361797at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006546">
        <property type="term" value="P:glycine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042743">
        <property type="term" value="P:hydrogen peroxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006550">
        <property type="term" value="P:L-isoleucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.30:FF:000001">
        <property type="entry name" value="Dihydrolipoyl dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000001">
        <property type="entry name" value="Dihydrolipoyl dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050151">
        <property type="entry name" value="Class-I_Pyr_Nuc-Dis_Oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006258">
        <property type="entry name" value="Lipoamide_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01350">
        <property type="entry name" value="lipoamide_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912:SF151">
        <property type="entry name" value="DIHYDROLIPOYL DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912">
        <property type="entry name" value="DISULFIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
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        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
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    </dbReference>
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        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
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    </dbReference>
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        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 9 11" id="KW-0274">FAD</keyword>
    <keyword evidence="2 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 9 11" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 11" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="25"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="12">
        <location>
            <begin position="371"/>
            <end position="480"/>
        </location>
    </feature>
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        <location>
            <position position="469"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="72"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
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        <location>
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        </location>
        <ligand>
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        </ligand>
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        </ligand>
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            <begin position="204"/>
            <end position="211"/>
        </location>
        <ligand>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="227"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="296"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
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    <feature type="binding site" evidence="9">
        <location>
            <position position="337"/>
        </location>
        <ligand>
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        </ligand>
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        <location>
            <begin position="343"/>
            <end position="346"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:57692"/>
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        <location>
            <begin position="63"/>
            <end position="68"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EGW33948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="490" mass="52825" checksum="D54A6360E9BCA52F" modified="2011-11-16" version="1">MLRTTNFKPVTSKFSQFVRYASTKYDVVVVGGGPGGYIAAIKAAQLGYNTACIEKRGALGGTCLNVGCIPSKSLLNNSHLYHQVKHEAGIRGISINGDISVDFPGLMSAKEKAVKQLTGGIEMLFKKNKVTYLKGEGSFVNEKTLAVKPIDGSETHEVEADHIIVATGSEPTPFPGIEIDEERIVTNTGILALKEIPKRLTIIGGGIIGLEMASVYSRIGSEVTVIEFQSALGSGMDGEVAKNVQKFLGKQGLNFKLGTKVTKGVRNGEVVNIEVEDVKSGKKEEIESDVLLVAIGRRPYTEGLNIEAAGLEADNKGRLIIDEQFRTKHPHIRVIGDVTFGPMLAHKAEEEGVAAAEYIKHGHGHVNYGNIPSVMYTHPEVAWVGLNEEQLKEQGIKYKVGKFPFIANSRAKTNVDTEGFVKFLADAETQRVLGVHIIGPNAGEMIAEAGLALEYGASTEDIARTCHAHPTLSEAFKEAALATFDKPLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
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            <dbReference type="EMBL" id="EGW34011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="64" mass="7389" checksum="BC60622A63CDFEC0" modified="2011-11-16" version="1">MSEVSDNPRANAHLISASSWVAIQLDIHYITIISFSQQLAVPVFQTLNFRSERDGAWHQHRVWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJW1</accession>
    <name>G3AJW1_SPAPN</name>
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        <recommendedName>
            <fullName evidence="5">Extracellular membrane protein CFEM domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">SPAPADRAFT_59424</name>
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    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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        <lineage>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
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                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
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        </source>
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        </location>
    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34012.1"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="309" mass="31662" checksum="73041AE98826408D" modified="2011-11-16" version="1">MKFLSVLAASVALASASNVSKYNALKNMMKREDACSGSCIEMQNTVDTCYPNDSDDDDPTVLACVCGLDDTFYDQLSDCAQNCQHISAEASREFGGQLDPASLRKFYCNQAAQASAGDIPTGANGDQDYPAATDEATFAGEDSGDDSGNGYFSAAATGIYTDANAGSDSNAGGRWATAYTSGGTVILTYIQNSIATDANVAEIAPTQSVSKSASKSDIKQTTAAAAAQSEQSTIASSSTEQPKSESSITTTSAASASKTEQSSSTTTAQSSSKEQTSSKTESTSAETKTTNGAETVGVSLISLMAIALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
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    </protein>
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        <name evidence="3" type="ORF">SPAPADRAFT_134029</name>
    </gene>
    <organism evidence="4">
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TCP11 family.</text>
    </comment>
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        <property type="protein sequence ID" value="EGW34072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007373594.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="G3AK21">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK21"/>
    <dbReference type="GeneID" id="18869775"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134029"/>
    <dbReference type="eggNOG" id="KOG1981">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016970_1_0_1"/>
    <dbReference type="InParanoid" id="G3AK21"/>
    <dbReference type="OMA" id="CAPMRDV"/>
    <dbReference type="OrthoDB" id="276323at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010737">
        <property type="term" value="P:protein kinase A signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008862">
        <property type="entry name" value="Tcp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832:SF11">
        <property type="entry name" value="LD23868P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832">
        <property type="entry name" value="TESTIS-SPECIFIC PROTEIN PBS13 T-COMPLEX 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05794">
        <property type="entry name" value="Tcp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="643"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="643"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="659"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="674" mass="76814" checksum="073D9AE692AB9A23" modified="2011-11-16" version="1">MNSQVNSDQQEQQPAVFQEEQQEQQQQQQQFRQVCFNPATAAAAAAAVAATTVATAGSSSTTPSDNSAPVTTSNKRNSSSSSDPAKRIRLDTSASGSTTNNITTPKFIRRFRSRSLPIINFSNHAATSATTSTFYPHKKHHNLFSTTNHHINSNSSSNSSSNSTIPPLPPINLQSLKEIDLHEILKNPQLRHDILFDPQLQFRPNLDGERGKRKKSIIDKYWLEIQKECSQFFNRQQNVAIKLNRLPILFTTLRDILLSLLPTKDRQHVEEILDIELLIQQLNHGSFDFVEMSNWLGEVFKNHCAPMRDQWVSEMNEKFVKSYTLNNVEYLVEGLRMIFQILEAMKLDVANHQIRILRPVLIETAIDFERDYFQTLINHGKININDSLNWFYKKYMKKITPDAQTNINDTTLKPIIISSIIDLLSCRQMATEFPSTLTFDHTRLVLLRADVRQLVCIQLCVILYKQLIINSKVPNSSELLSSANISKVQQDILAIVTDDNGNIKWTKNISSISLQLVKYLYPQQISQVNLPKDLIDFSYNWLIKHIQPNSQVYGLMEIKIFNQLLSEIVNIIDGVDERELELTKSAPNNTTEMKNIATRIATLVKFHWNVFGNYYIDYIKSQHVKLQQDNTIMNTGLVIKHNEEDGNKARGNGGERSHTNSIDNNASAPLSMFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK41</accession>
    <name>G3AK41_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">inositol phosphorylceramide mannosyltransferase</fullName>
            <ecNumber evidence="8">2.4.1.370</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_60194</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1D-myo-inositol-1-phospho-N-[(R)-2-hydroxy-very-long-chain fatty acyl]-(R)-4-hydroxysphingoid base + GDP-alpha-D-mannose = an alpha-D-mannosyl-(1&lt;-&gt;6)-1D-myo-inositol-1-phospho-N-[(R)-2-hydroxy-very-long-chain fatty acyl]-(R)-4-hydroxysphingoid base + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:64596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:155885"/>
            <dbReference type="ChEBI" id="CHEBI:155926"/>
            <dbReference type="EC" id="2.4.1.370"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:64597"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.370" evidence="8"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374367.1">
        <property type="nucleotide sequence ID" value="XM_007374305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK41"/>
    <dbReference type="FunCoup" id="G3AK41">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="GeneID" id="18873267"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60194"/>
    <dbReference type="eggNOG" id="ENOG502QS3D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036369_3_2_1"/>
    <dbReference type="InParanoid" id="G3AK41"/>
    <dbReference type="OMA" id="EYLFYSW"/>
    <dbReference type="OrthoDB" id="3647at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031501">
        <property type="term" value="C:mannosyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103064">
        <property type="term" value="F:inositol phosphorylceramide mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006676">
        <property type="term" value="P:mannosyl diphosphorylinositol ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051999">
        <property type="term" value="P:mannosyl-inositol phosphorylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.20:FF:000001">
        <property type="entry name" value="MIPC synthase subunit (SurA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051706">
        <property type="entry name" value="Glycosyltransferase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32385">
        <property type="entry name" value="MANNOSYL PHOSPHORYLINOSITOL CERAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32385:SF20">
        <property type="entry name" value="MANNOSYL PHOSPHORYLINOSITOL CERAMIDE SYNTHASE CSH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="390"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="403"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="430" mass="49294" checksum="730457A3D327FAE2" modified="2011-11-16" version="1">MRKELKYIIWGHVALILYLAYLSFDLITLLHDDSFSDALLDVELNPSDGKIDRPAIIPKIIHQTYKTSDIPEIWKSGQQACVSLHQDYQYILWTDAKARQFISEEFPWFLKTWDSYPYPIQRADAIRYFALVHYGGIYIDLDDGCERKLDPLLTVPAFVRKTVPTGISNDVMGSVPNHPFFLKVLESLKTYQRNWLVPYITIMFSTGPLFLSVMWKQYKRWGVPEAGKVRILMPQDYKASPYSFFAIAPGSSWHMDDAKFVKGLANHLVLAVVGGFLIAFLIFYLEYLFYCWVISPTFKKMMGKITGGACFVMNAVLRVLKLDKFIESWTSYEPVVEETANNNNKKKKVDLIGLLSYNHSIKRRSRKDSNLPIELGVLEKLADKDTIVVDSAGESGQSQTRPTSDYSNTNTDNESNDTETYVFKNNDNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK53</accession>
    <name>G3AK53_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptide:N-glycanase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_60205</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transglutaminase-like superfamily. PNGase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374379.1">
        <property type="nucleotide sequence ID" value="XM_007374317.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK53"/>
    <dbReference type="FunCoup" id="G3AK53">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK53"/>
    <dbReference type="GeneID" id="18873274"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60205"/>
    <dbReference type="eggNOG" id="KOG0909">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031058_0_1_1"/>
    <dbReference type="InParanoid" id="G3AK53"/>
    <dbReference type="OMA" id="NDFFTWI"/>
    <dbReference type="OrthoDB" id="409136at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120125">
        <property type="term" value="C:PNGase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000224">
        <property type="term" value="F:peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097466">
        <property type="term" value="P:ubiquitin-dependent glycoprotein ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.620.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050883">
        <property type="entry name" value="PNGase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018325">
        <property type="entry name" value="Rad4/PNGase_transGLS-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12143">
        <property type="entry name" value="PEPTIDE N-GLYCANASE PNGASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12143:SF19">
        <property type="entry name" value="PEPTIDE-N(4)-(N-ACETYL-BETA-GLUCOSAMINYL)ASPARAGINE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03835">
        <property type="entry name" value="Rad4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Transglutaminase-like" evidence="6">
        <location>
            <begin position="173"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="333"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="339"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="367" mass="42771" checksum="4389D322A77F27CE" modified="2011-11-16" version="1">MTSIDYSNLADRLITIYAKQKIVSDRKKFRINHAELQESLVLPVCQSIHVHANCVDVYKNPNYLNEVLDTIDLATIYMNVDKRENEPDKNPNLKYDDFVVLELLQYFKHNFFTWVNTPKCPNCQDEKNVESKGSIPFNRTLPNPDEISIVEHYQCFQCGTNIQFPRINNPVSLLRTKQGRCGEWVNCFMLILQALIGEDKDRIRYVWNQEDHVWCEYYSYGLNRWVHLDPCEAVYDEPLLYCNNWGKRMSYVIGFNQNYIIDLSDKYITPEKQIEKSSVISKNKVDKLINYYNSNKLANYYNQQVSKLGNEHKALLSVYNDIIIPRNQELDVMKEPNKPSATSTTLTKGRQTGSAAWTKSRGEDGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKK2</accession>
    <name>G3AKK2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_136778</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375122.1">
        <property type="nucleotide sequence ID" value="XM_007375060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKK2"/>
    <dbReference type="STRING" id="619300.G3AKK2"/>
    <dbReference type="GeneID" id="18869997"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136778"/>
    <dbReference type="eggNOG" id="ENOG502QPY2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011075_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKK2"/>
    <dbReference type="OMA" id="TAQAYRW"/>
    <dbReference type="OrthoDB" id="3886144at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF15">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="15"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="167"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="684"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="172"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="684"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW33607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="756" mass="83970" checksum="E8E0E06533664574" modified="2011-11-16" version="1">MPKSDSRPRTKSRNGCHTCKRKRLKCDETKPACMNCRKRKIECGGYAVNFKWKSFNETETAATTTSATPHSSGSDHESPIATKNNVELKQHLELVSISVTGKSINEINKENHLISLGLNPNSYKRKQSHETNVIEEEPAPSALSTPVDSNNTKLNSLVEAATSIIDKPGASLPNTPGSASSVFSTSEFASDKTKPTDLSPQYDFGATPSSFSAIVNFAFEDIDALNLIESQTPTSTAVVPAKPTFDLHPINNSLIHTSEEQHLLFLYSEYTCHILSIKNGPRENPWRELILPKAMKYPCLYNSIASMTLFHLSGSTSAQAADMRAKGYSYMKKCVFDLLKGLSNTKPEESTLPADIALVTSLNLAFIDAWYTPTTSGIAHLRGAKSMIERVLKILNMEKQDSDKECDKLVFVDKYEEQLGASQTSGKLYIPKSIQFLFNVWIYLEVLAQMTANLNQDDKGVDLVAAITAMSQPMSKSESAESTPSTDPFTISPLFDNFSSTNYTPEFIDPLLGCGQSLFSIMGKVANLSTKIRKSKSTRNSLITISTASNLKQQLINWKPPITSTDQDFPSHNDSTWDIPQCIATAESYKYATLIYLHHSVPEIPSLSSHKLAQKIFILMASIPEDSNMHVVLIFPLLVASCEAMPGEEREWCIDRWRILFEKMGIGNVERAFDIVKEVWKRKDQDDSEDSKNASENNSAHSSVKENEGEGEDYDQLSGLMAVLNDSESLLQTRGINSRTHWSMVMKEWGWEVLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKS6</accession>
    <name>G3AKS6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_136690</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374495.1">
        <property type="nucleotide sequence ID" value="XM_007374433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKS6"/>
    <dbReference type="FunCoup" id="G3AKS6">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="GeneID" id="18869992"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136690"/>
    <dbReference type="eggNOG" id="ENOG502T5AY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_993951_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKS6"/>
    <dbReference type="OMA" id="HRSWIEM"/>
    <dbReference type="OrthoDB" id="4016272at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="264" mass="31234" checksum="AD1487A47CF90674" modified="2011-11-16" version="1">MLILLVWLVRLIVALPDSSQSCITNKNIPFTGYKIEYQLRLNTFQCEYHMIYCNDNKLIYDMNLNDTIPWTHPIHFSKTNRSNTLILARRKSFALRCIPIYLPNTLVGSLYYNPTSQPVTNNTKLQSVPLVAANIHRSWIEMNYKLEFTYHVPLLTTPGIDSRRFQIGMFKEQNKNNWNEYISITESYDFKYDSRIVVRKAIDRVLTQINLLINTSKFVKKKIDMSNISKRTSRVFWKLVMLSKVLKHETFDMILVKIDKFLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKW2</accession>
    <name>G3AKW2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_60329</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374520.1">
        <property type="nucleotide sequence ID" value="XM_007374458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKW2"/>
    <dbReference type="GeneID" id="18873347"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60329"/>
    <dbReference type="HOGENOM" id="CLU_2777538_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKW2"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="69" mass="7789" checksum="7BBCD60D0D6D9D6F" modified="2011-11-16" version="1">MSIYKKWMALPANGRYTIAGLTMVLAYAGDYLCTKLWEEQEARAEVLKRVEEQRAKQQGAPAVAAEKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKW5</accession>
    <name>G3AKW5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosome maturation protein SDO1/SBDS N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_60332</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374523.1">
        <property type="nucleotide sequence ID" value="XM_007374461.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKW5"/>
    <dbReference type="FunCoup" id="G3AKW5">
        <property type="interactions" value="163"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKW5"/>
    <dbReference type="GeneID" id="18873348"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60332"/>
    <dbReference type="eggNOG" id="ENOG502S9SB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137480_1_2_1"/>
    <dbReference type="InParanoid" id="G3AKW5"/>
    <dbReference type="OMA" id="PEHGKQG"/>
    <dbReference type="OrthoDB" id="2567806at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1250.10">
        <property type="entry name" value="Ribosome maturation protein SBDS, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036786">
        <property type="entry name" value="Ribosome_mat_SBDS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019783">
        <property type="entry name" value="SDO1/SBDS_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01172">
        <property type="entry name" value="SBDS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89895">
        <property type="entry name" value="FYSH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS N-terminal" evidence="2">
        <location>
            <begin position="3"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="113" mass="12659" checksum="1CF7B1810B8EB704" modified="2011-11-16" version="1">MANPHKVLYKGQEVDFILFVDDIELLKKYRKGDTTIPLVDLVSVYKVFSNRNRGSGGIFDQASKGDLENEFGKTSVDDIIKKILIEGEDKKDVHLNPEYSSKNDSHGGRDSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL01</accession>
    <name>G3AL01_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_66055</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374559.1">
        <property type="nucleotide sequence ID" value="XM_007374497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL01"/>
    <dbReference type="GeneID" id="18875078"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66055"/>
    <dbReference type="HOGENOM" id="CLU_390369_0_0_1"/>
    <dbReference type="InParanoid" id="G3AL01"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="551"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="599"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="631"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="679"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="707" mass="81844" checksum="78CB6E50F7033C65" modified="2011-11-16" version="1">MTLSKNRGTDTDWHGLALSHFSIASGISSQIKNQDLTGNRAPIHNRDTNMSSDPELKYIDNRWLYFHGLMIDQTSRQIVLNTPNTIVDQLGDTQIRQLARKQRALKSSKFLHFKYPSLIEFVAVEPLYLKYCQDEFLVRRKEQIPEWELLKGKKVLGTEVVNERMKVVIYIDEVDDTPIPKESVRYWKSERILEDCYQSIQFSQSYSESITRSLNSTYKNEGIYLYSKITHERNNKQKLNTTIQSCLERSIDEQNSNMREMSIYYFDCFSLFYQILVCVDLDLIMGETKTKIETLKTPQLDLDVKQQALKDLFVDFMSKNIILFVQILVPYLVNLEDSTFLDCFDCLKRLDVLKNFAISCMLLELDQGIFISLMSSASTTLNDKYEGWRILQIIYEQKKKEVLQNILENGNSKNIELPSLAELNATHDMAPFVEQTANSPLDAAGSRYKETSISATDNSASLEQIVILSDSEDEQDAITTVPAPVSTSVPEPAPAIENDSLLPLSSESPDKYCKGPDFSGLYSCSYDGCDFQSYKRFDMRKHIKRHLGTRYRCIQCLKKFDDIPKLESHVESHEVLVERVLPQHPLDEYCHGPGPRCIYTCIYDDCGERITFRVNMHLHIQSHLQESNLPIECLHCCKRFKSIKLLSTHLRFNHSDILEQFTHVQFKNYYNDYNKVNGYKCTYNNCGTCFTSKLVMEHHITKHLNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL97</accession>
    <name>G3AL97_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CUE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_60448</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374655.1">
        <property type="nucleotide sequence ID" value="XM_007374593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL97"/>
    <dbReference type="STRING" id="619300.G3AL97"/>
    <dbReference type="GeneID" id="18873417"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60448"/>
    <dbReference type="eggNOG" id="ENOG502RV3A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_721922_0_0_1"/>
    <dbReference type="InParanoid" id="G3AL97"/>
    <dbReference type="OrthoDB" id="5577209at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14373">
        <property type="entry name" value="CUE_Cue3p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052586">
        <property type="entry name" value="ASCC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041808">
        <property type="entry name" value="Cue3_CUE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21494:SF0">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21494">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 2 ASC-1 COMPLEX SUBUNIT P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00546">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="CUE" evidence="3">
        <location>
            <begin position="63"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="111"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="190"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="300"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="130"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="321"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="340"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="361"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="383" mass="42667" checksum="767A6AED12BC0B3B" modified="2011-11-16" version="1">MELGITSINSLELSPLFSSIIISDAYKELVPSLEERLPFLRSIKFEEDEDEDEDDEDDDGFEQNIEGISLLVDLFPQLTEAKAKYILKENNGDSEHVTNLLLENPDMIDSIPEQAPKKKIKKEKSTPKVTVKKPQGKAAKRSIYDDDKISKGDFSGTTIIFGKKDRDKIEPASADLKKKTLSAALRLMYESDEDEPDDTYEDQEKTTGAALEDTKKKPSGKMAVLEDESSGAIGSGIDERERFLFSVFKRQGPESFEKTKRKSSSRQELKKSTNWSDEQIEGWLRMLLKSPRRFKLLEEDFFYGGGNPNRRTKKVVEDESASTASSLSPSPVPSASGSSTPQKKSGASSKRTQARNERNKASKANHNRKAGHDKKAKAAGAGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL99</accession>
    <name>G3AL99_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">TATA-binding protein-associated factor mot1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Modifier of transcription 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_137527</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374657.1">
        <property type="nucleotide sequence ID" value="XM_007374595.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AL99">
        <property type="interactions" value="1412"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL99"/>
    <dbReference type="GeneID" id="18870050"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137527"/>
    <dbReference type="eggNOG" id="KOG0392">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_1_2_1"/>
    <dbReference type="InParanoid" id="G3AL99"/>
    <dbReference type="OMA" id="WYSDIAC"/>
    <dbReference type="OrthoDB" id="10252227at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045898">
        <property type="term" value="P:regulation of RNA polymerase II transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17999">
        <property type="entry name" value="DEXHc_Mot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000009">
        <property type="entry name" value="B-TFIID TATA-box-binding protein-associated factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000428">
        <property type="entry name" value="TATA-binding protein-associated factor 172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044972">
        <property type="entry name" value="Mot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044078">
        <property type="entry name" value="Mot1_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022707">
        <property type="entry name" value="Mot1_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36498">
        <property type="entry name" value="TATA-BINDING PROTEIN-ASSOCIATED FACTOR 172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36498:SF1">
        <property type="entry name" value="TATA-BINDING PROTEIN-ASSOCIATED FACTOR 172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12054">
        <property type="entry name" value="DUF3535"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="1331"/>
            <end position="1504"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="1686"/>
            <end position="1837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="200"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="711"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="291"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="721"/>
            <end position="736"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00081329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW33142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1924" mass="214931" checksum="02E4E775036C80B6" modified="2011-11-16" version="1">MSRLDRLVVLLETGSTPFIRNTAADQLSDLAKAHPEDIISLLGRVYPYLKSPKWETRISAARAFGGIVNNAGLWNPNAEVKTEETENNQEEDVPIKQEEAVIKEEEIPEIKQEEEEVKIKLEQDEELKKLEDNLSNLVTFSAFNLHELIKSGNRLLAAKSNDDEPEATNGNGTTSDDDTSLMAKIKRHRLNVIKQEEQEIKQETKEEQDQQISSSTPSIANTPSGSAQPTPSTTPAPSKPISSARLKAMQKRRAKVNAKSNANRVKTVDISQSSLSRQMIENGETDMDVDSSGNETPPASTPQFDVTSQQGGEKLVVETKAAPELSPLLSLHNKVAGLIWQFQGVYELLLADLFDDRWEIRHGACLGLRELVRKHGQGAGRVSKRSKAENDHNNSATLEDLAVRLCILLCLDRFGDYVSDTVVAPVRESGAQTLAALLIHLDDETVIKTFDCLNTLVLQPDLVPRCWEAKHGGMLGVRYLVSVRTDILVKNPGILDSVVNMVLHGLRESDDDDVQSVAALTLTPIASEFVTTRTNLVSTLLNVIWDCLVNLRDDLSASIGSVMDLLAKLCSHHEVIEIMQQGAADDSSNSFETLVPRLFPFLRHSITNVRKAVLRTILEFLSLDDPTTKRWIDAKTLRLIFQNLLVEQNNDVLTLSAEVYDKILLEMKNMDLDIGSIFLSQSQPLLTLTMTPIGISRHNYQMNTALIKRPSGQSLGSWDDEDKRGRKRKPEEPKTDIPIEDLKVNIDAPIYRGDVMLVGYDKFIATRCAAVHAFGNTLSYIESEEILLEIFTNVLHYVKSSNHATSRLLGAFIIEEYSDSVKDRSQDVNPQVVSMFQESLLHVLQSESTLPHFRELVPTLKSVRTSCLQLFDVFINTAKLAPSKIPQMPVVVQGESDAGPGAFGLENAEKLCSEIFPKLKKSLSTSYRLTANQALDDAKHRITIAIDEAKSASNSRVTSIMAAFASAVLALSGVPPKLNPVIKSLMESVKSEETSVLQKRSVRAMARLIKELKVAGKQGAADKIVKNLCAFLCVDTSEVPEFHHNVVFKDNILSLRKEEAKTDPADISAHEKAVHEARIKRTGALLTLEQLLKIYDAALFENVPKLKEIMIEPLNLLNQASNESIVKDELKGQSIVDALGILRALLPKMNKSLYPQITDNLPLLLPGLTSEYSVFRYSTAKCLATICSVVPAKTFTFLVKSVLPMLNNAGSVKDRQGAIETIYHIATTMGPEILPYIVFLIVPVLGRMSDADHDVRVLATTTFAGIIKLVPLEAGIPDPEDMPEDLLAGRDRERDFIQQMMDPTKIKPFDLPVTIKATLRKYQQEGVNWLAFLNKYHLHGILCDDMGLGKTLQTICIVSSDHHLRAEKFEKTQSPQYRRLPSLVICPPSLTGHWEQEINQYAPFMKVLIYAGNPSVRIPLRKEIPFADVVVTSYDVCRNDVEYITSQDYNYCVLDEGHIIKNAASKLTKSVKKVRAEYRLILSGTPIQNNVLELWSLFDFLMPGFLGTEKVFHEKFAKPIAASRNSKTSSKEQEAGALALESLHKQVLPFMLRRLKEDVLSDLPPKIIQDYYCELSDLQRMLYKDFAKSQKEKVKEDISSQEKEVKTHVFQALQYMRKLCNHPALVLSPQHPKFNEITHWLQTKKSDLRSIEHAPKLLSLRNLLLECGIGVGDHDYFKSKSKQQNQLISAEGVISEHRALIFCQLKDMLDIVEKELLKKYLPSATYMRLDGSTDPRDRQSIVRKFNEDPSIDILLLTTKVGGLGLNLTGADTVIFIEHDWNPMNDLQAMDRAHRLGQKKVVNVYRLITKDTLEEKIMGLQKFKMNIASTIVNQQNSGLQSMDTNQLLDLFDVDESAAGASKISLDDESSNMKIDDKNENANKAEDLAGGLTGKAAGAVGELADLWDESQYEEEYNLENFIKTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALG7</accession>
    <name>G3ALG7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_60540</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the YIM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374725.1">
        <property type="nucleotide sequence ID" value="XM_007374663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALG7"/>
    <dbReference type="FunCoup" id="G3ALG7">
        <property type="interactions" value="148"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALG7"/>
    <dbReference type="GeneID" id="18873457"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60540"/>
    <dbReference type="eggNOG" id="KOG1198">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_3_1"/>
    <dbReference type="InParanoid" id="G3ALG7"/>
    <dbReference type="OMA" id="GPLTYFT"/>
    <dbReference type="OrthoDB" id="3509362at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050700">
        <property type="entry name" value="YIM1/Zinc_Alcohol_DH_Fams"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11695">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11695:SF294">
        <property type="entry name" value="RETICULON-4-INTERACTING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="4">
        <location>
            <begin position="26"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW33210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="359" mass="39295" checksum="53254FB145D095B5" modified="2011-11-16" version="1">MVFDSEILNYKTYAYSNANHPVELVDQSIKLVKSFTGQGYTIPANNILVKIHYAALNPVDIKMYHLAFGITSLFNSKKGFGKDYCGEVVAVGSEADTTVKVGDFISGLYFPVYAQGSVGQYLMLDTTKKQERVFTTVALNLSHAESASWPLVLGTAMYMLNGLELANKKVLVLGGATSVGRYVTQLLKVENSKEIVATCSPRSEKLVIELGATSTIDYHGNVLNSVLESVKTSGAFSYIIDCTGGDELFRNISIILQKGGTYNTIVGDKPGSSVLNLAVSIVKGMTRVVASKLGLISYTYNYMLLEPGFNFMDEAKKYLENGDVKVFVDHIYKFDEFNQALKRLESGNPSGKVVIEIDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALU9</accession>
    <name>G3ALU9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF803 domain membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_60063</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374222.1">
        <property type="nucleotide sequence ID" value="XM_007374160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALU9"/>
    <dbReference type="STRING" id="619300.G3ALU9"/>
    <dbReference type="GeneID" id="18873205"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60063"/>
    <dbReference type="eggNOG" id="KOG2922">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012349_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALU9"/>
    <dbReference type="OMA" id="PMVYISI"/>
    <dbReference type="OrthoDB" id="6428174at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF85">
        <property type="entry name" value="DUF803 DOMAIN MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_1G15880)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="145"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW32707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="39827" checksum="4F7AA11A7C6B3080" modified="2011-11-16" version="1">MMEDKYIGLALAMSSSLAIGTSFIITKKGLMDASARNGNNQVQGHEYLQNPIWWGGMITMAIGEIANFAAYTFAPAILVTPLGALSVIIGAVLAAIFLKEELGTLGKMGCAICLMGSVIIILHAPPDKEVETVDEILGYATQPGFLFYCTLVALYSLFMIYKIVPKYGNTNPMIYLSICSSVGSISVMSIKAFGIALKLTLGGNNQFTHVSTYLFLLVVVGCIITQMNYFNKALDQFDTSIVNPLYYVTFTTFTLAASFILFRGFNTSSAVDIISLLIGFLIIFSGVYLLNIARKEDPSKNREIFGVHASKDMAPMDNGVGGFTTVRRSMQINRAEYDTEETVGLRRFDSFELSDSDSEGSRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM23</accession>
    <name>G3AM23_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">hydroxymethylbilane synthase</fullName>
            <ecNumber evidence="3">2.5.1.61</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60083</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>dipyrromethane</name>
            <dbReference type="ChEBI" id="CHEBI:60342"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HMBS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="3"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374243.1">
        <property type="nucleotide sequence ID" value="XM_007374181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM23"/>
    <dbReference type="FunCoup" id="G3AM23">
        <property type="interactions" value="665"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM23"/>
    <dbReference type="GeneID" id="18873213"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60083"/>
    <dbReference type="eggNOG" id="KOG2892">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019704_1_0_1"/>
    <dbReference type="InParanoid" id="G3AM23"/>
    <dbReference type="OMA" id="NQYIGSK"/>
    <dbReference type="OrthoDB" id="564646at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000005">
        <property type="entry name" value="Porphobilinogen deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557:SF0">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="6" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Porphobilinogen deaminase N-terminal" evidence="7">
        <location>
            <begin position="8"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="222" mass="24206" checksum="279E18E9C3AE935C" modified="2011-11-16" version="1" fragment="single">MSVAPTHIQIGGRKSKLAVVQSEAVKAAIEAKFPSISCSILALSTLGDKVQSLPLYAFGGKSLWTKELEVLLLQSIDEYPQLDIIVHSLKDMPTNLPEEFELGCIFHREDPRDAIVMKAGSPYKSLAELPDGSIVGTSSIRRSSQLVKNYPKLKFESVRGNLQTRLGKLDDPENEYCCLILASAGLVRVGLGDRITANLDDMYYAVGQGALGVEIRNGDEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM81</accession>
    <name>G3AM81_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_60731</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374894.1">
        <property type="nucleotide sequence ID" value="XM_007374832.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM81"/>
    <dbReference type="STRING" id="619300.G3AM81"/>
    <dbReference type="GeneID" id="18873541"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60731"/>
    <dbReference type="eggNOG" id="ENOG502QTI4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_848775_0_0_1"/>
    <dbReference type="InParanoid" id="G3AM81"/>
    <dbReference type="OrthoDB" id="5599171at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071595">
        <property type="term" value="C:Nem1-Spo7 phosphatase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005605">
        <property type="entry name" value="Spo7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28249">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN SPO7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28249:SF1">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN SPO7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03907">
        <property type="entry name" value="Spo7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="328" mass="37140" checksum="9CA174F1D391B7B4" modified="2011-11-16" version="1" fragment="single">MDHTAQDQSIQDPQPSGSTNENTYDDVQNTSRDTLIHLPADTPPSSSPSSPCEDRDQLIFTPPRSPYLRSSDRMNITPDSPTDSPRLDEFVMYSDSDVASQSSLDLGTTPKATRRKLVKVSSDEEEYDEEEIADRVRYYDEQGRGVTLEPPSEKPRRRKRSSVGRKKTVQTESTNNPKSSKKKIRRQSRSKEPSERSRSDFIPGTGYTSVPATGKVFRNLLILEESLREQVIQQRAMRRKYLTFLALLCSIIASLIHHLFILDPSVSSTGTTRVILQFLLLASIITLILYHLSGEYQKTIVLPRKFLSSTNKGLRQLNVRLVKIKTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMC0</accession>
    <name>G3AMC0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mannosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_60774</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the eighth mannose residue in an alpha-1,6 linkage onto Man(7)GlcNAc(2)-PP-dolichol to produce Man(8)GlcNAc(2)-PP-dolichol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29535"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="Rhea" id="RHEA-COMP:19518"/>
            <dbReference type="Rhea" id="RHEA-COMP:19519"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:132517"/>
            <dbReference type="ChEBI" id="CHEBI:132519"/>
            <dbReference type="EC" id="2.4.1.260"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:29536"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyltransferase 22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374933.1">
        <property type="nucleotide sequence ID" value="XM_007374871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMC0"/>
    <dbReference type="GeneID" id="18873562"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60774"/>
    <dbReference type="HOGENOM" id="CLU_1901329_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMC0"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052917">
        <property type="term" value="F:dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF1">
        <property type="entry name" value="DOL-P-MAN:MAN(7)GLCNAC(2)-PP-DOL ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="74"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW33418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="134" mass="15043" checksum="212B822B7A8E0DB4" modified="2011-11-16" version="1" fragment="single">MHSIELSRSFIGPLILASLCKCILSLTSSIPIINDNPWDENELSFLVSLLLGAGNVLSWTYLRNTLIRVTGKRKTGVWFSILIISQHIIFKCTRTLPLSFASPFVNYSYAKIIMGDLSGLVWLAFAATVFRYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMG2</accession>
    <name>G3AMG2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VHS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_60159</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374329.1">
        <property type="nucleotide sequence ID" value="XM_007374267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMG2"/>
    <dbReference type="FunCoup" id="G3AMG2">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMG2"/>
    <dbReference type="GeneID" id="18873248"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60159"/>
    <dbReference type="eggNOG" id="KOG1087">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036827_2_1_1"/>
    <dbReference type="InParanoid" id="G3AMG2"/>
    <dbReference type="OMA" id="YGSVHRQ"/>
    <dbReference type="OrthoDB" id="10068368at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14232">
        <property type="entry name" value="GAT_LSB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16980">
        <property type="entry name" value="VHS_Lsb5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044103">
        <property type="entry name" value="GAT_LSB5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045007">
        <property type="entry name" value="LSB5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47789">
        <property type="entry name" value="LAS SEVENTEEN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47789:SF1">
        <property type="entry name" value="LAS SEVENTEEN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VHS" evidence="2">
        <location>
            <begin position="20"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="361"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="240"/>
        </location>
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            <begin position="401"/>
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        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
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            <dbReference type="EMBL" id="EGW32814.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
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    </evidence>
    <sequence length="447" mass="50099" checksum="143A326C570A5A47" modified="2011-11-16" version="1" fragment="single">MPIFSDHAYTSITIKINQLAEHTNTDTDEDSLELYLSDLLELIKIQPSSGAKEAARAVRKKIKYGEGVQEQLRALQILELLVLNSGTAIGKIIARDDKLIDVLQGIIRGNGKTGDGVAYDKRVKSRVVAMAKGWKHELADMGDEYKYMQGLYKTIPGAKRRDRERERSERGNDREDSYDIPDDSRDAFNDESADVSPPRTPRRRRRETPRETPRDTPQDSAREIPKEDKKSKKKSKKKKGIVYADQEFEIPQINYKVEAPKIRDLLASSYTHSTTLSNLLLQLPGGESPLDNDKCLDEFQNCKKIRRKILHYLQYVGAGSSSAKSSEVAALDEEFLGSLISANEQLVEVFKKFDIKAGYNNAQNSVPTYGEESDSDSSYYTESDSEDSIEERMNHMNLNKSPPPPPKVRSPPPPRPSKPAALSKPVPVAQVDDNPFGDGNAVSHSIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMN7</accession>
    <name>G3AMN7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Actin cytoskeleton-regulatory complex protein PAN1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Actin cytoskeleton-regulatory complex protein pan1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">SPAPADRAFT_135776</name>
    </gene>
    <organism evidence="25">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Component of the PAN1 actin cytoskeleton-regulatory complex required for the internalization of endosomes during actin-coupled endocytosis. The complex links the site of endocytosis to the cell membrane-associated actin cytoskeleton. Mediates uptake of external molecules and vacuolar degradation of plasma membrane proteins. Plays a role in the proper organization of the cell membrane-associated actin cytoskeleton and promotes its destabilization.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the PAN1 actin cytoskeleton-regulatory complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Actin patch</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PAN1 family.</text>
    </comment>
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        <property type="protein sequence ID" value="EGW33481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007374934.1"/>
    </dbReference>
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        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMN7"/>
    <dbReference type="GeneID" id="18869916"/>
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    <dbReference type="eggNOG" id="KOG0998">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001619_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMN7"/>
    <dbReference type="OMA" id="GMPGQWG"/>
    <dbReference type="OrthoDB" id="2015333at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
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        </source>
    </evidence>
    <sequence length="1350" mass="146116" checksum="0B7E72410AD42913" modified="2011-11-16" version="1">MYNPYQQQQQQGYGFNPQQTGYVGGFNYQQQPQQPPQQMYQQPSLQGQMTGFIPQQPNLYSSGFPAQPQTSFQQQQQQQPPSLQPQATGYIQTQPTGFGQSAPTVVENSELKIPAIRLSFITAEDQKKFEHLFRTAVPKGEQAIGGDAASNILLRSGLPPVTLAEIWSLSDIGNTGSLLFPEFALSLHLCSKAKRGESLPGVLPEKWLNEVKSFSDAINFTVPDDPSKILANTPFASFAPPPPDTDWMSKPAPTSFQPQMTGFGAAPLAAQRTGPIAAPPTTSFGAALTAQRTGGGTLIPLQPQQTAGLIPAQKTGPLPGQLQQAPTQSFIPPQGTGFQPQPQLSSQRTGPMQPQRTGPLQVQRTGSLQAQMTGGLVAQPTGRPGEWGFVSMPTGGIPGLNAMQQHFLPNAQLPSTNLQSAMDTSLSSNVTWAITKQEKQIYDGLFEAWDTKRRGYIDGDVALNVFSKSGLARPDLESIWTLADTNDSGKLNKDEFAVAMHLVYRRLNGFDLPLRLPPELVPPSNKYLQDSMNTLKNSLKGGASKPAVPPKPQTKPDGTRFKNDDNNFSYVSNARHKRRGTTPELESKPSALKTSSDSGLTIDEMKKLIREKRILLDAMDVEDQDKPKVSRDTAEIENYKRRIMDVQSKLDEYEGGSSIDDRKQLLAKLDNLTRDRVPSLISSIQQMNQEIARKKIELIKLKLQRENPSWNPDDYESEIVGTGPNGEVTDRDRRKFHSKQQFKQKMAALTGRGSSSGGNADLDFKLKEGSEQAREECKRQSDMVGDIESGIKEMEDECASKLKVSVKEDAGSDKWERGLGVSEEVAKFVKELDAFSNSQRRNIAKAKQQSPVEYSSSKSSSPLVTTSSTGSITIDKYSTPEERAAYIKAQAEKKMNERLAKLGITRRKTSAADVNPPVESHPEPKEVSTPVVPKAEPKVEPPKIEPKAEPKSIPPAQKIVPAKAVPAPQEESSDDDDDEEYKAMLKQKQEMEARERERKLKKKQQKEERLAKLKREMEEMKKREAEDSDEDENENVTSVPTYVSKPKEEPKPGKVEPAEPVKPVEAAPVAHQPHESNPFAKKMAGGAEEAKTNPFFKPANKEVVVDTKKAAAQRASQRGISNNDGWSDSEDEAASEDESPNRAGAAQLASLLFGGMSQPSRSDSNLGKSDSGSKLPSPEAIKKEEPQILRPPEDNVSAGSSQFGTPTATPPPPPPPVFSAPPPPPVAVDVPAPPSVADIPPPPPPPPPPAFDAPPPPPPPPPPTLDGIPPPPPPPPPASFAPPPPPIPNGFNAPAAPPPPPPPPPPAAGDAAAAGGAPNISALLGQITGGKSLRRVETKEASGAVVGRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN06</accession>
    <name>G3AN06_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61489</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375696.1">
        <property type="nucleotide sequence ID" value="XM_007375634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN06"/>
    <dbReference type="STRING" id="619300.G3AN06"/>
    <dbReference type="GeneID" id="18873917"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61489"/>
    <dbReference type="eggNOG" id="ENOG502QWIS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095040_0_0_1"/>
    <dbReference type="InParanoid" id="G3AN06"/>
    <dbReference type="OMA" id="CDINYLL"/>
    <dbReference type="OrthoDB" id="3598904at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF46">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="56"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="241" mass="26653" checksum="A39C41EB2ADEFF42" modified="2011-11-16" version="1">MTSYRSYQQVLDISIKQKSNKTNVASVTSSAITTATKKITKVKKDDSNIKRRTKTGCLTCRKRKKKCDEDKVNGKCQGCTRNFLECCWPEAAPVAPATTKELASITKSPTLRSTKCNISSLLATPIKTEVTQVKCEKSTKVEPVPYPSPISSPEFTEAKLAANHEVNVISLPPLFNVNYKLQSKDSNVRGTRTEEVKQEKPRIITAPVTTPLSPPRTHASVPTKFVITSFNSHKDLCQIPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN69</accession>
    <name>G3AN69_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">GPI transamidase component PIG-S</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_61017</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGS family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375189.1">
        <property type="nucleotide sequence ID" value="XM_007375127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN69"/>
    <dbReference type="FunCoup" id="G3AN69">
        <property type="interactions" value="779"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN69"/>
    <dbReference type="GeneID" id="18873682"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61017"/>
    <dbReference type="eggNOG" id="KOG2459">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010026_1_0_1"/>
    <dbReference type="InParanoid" id="G3AN69"/>
    <dbReference type="OMA" id="WGGIFIY"/>
    <dbReference type="OrthoDB" id="28748at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019540">
        <property type="entry name" value="PtdIno-glycan_biosynth_class_S"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21072">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21072:SF13">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10510">
        <property type="entry name" value="PIG-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="467" mass="53144" checksum="14A92BA5290BCCBC" modified="2011-11-16" version="1" fragment="single">MSEHEKEPSAVKEEQKVEKQQPEDSPLVISLESKIITKTRTIIAVIIFSTIILLGIPLFIGTTTIHRAHLPIDQINQFDYKSQLHFKIPVSLSTDEGITAGNQQVYDQQLRTRYPDLNIWSIEIVNSINPDNYQVKVHLANKPSYEISLYDRQIDVFVDKDTNVDEYISSILYDSIFKYELDNLESNDHATVAFPYSSKFNLVFSLLVENGNPTSWEIEQASEKLQKLLISFNHISNFTITSQVQYYSKLSKKYQKGSSSYILPETDLPTFINFGDWNLDNYDINPTMNFLLYFPESNYEGIPLIIENTTTNSFLIPQWGGVAIFNKDKPILKTDAKLSSSDLEPIMNIFASQLYQLLGVPPTPKNPTIRIDSLSRLLVVSNIKKSLDNLVSLVKISDTLNEISIPDETKESADTSLEQINQAIELVKHGKFQFATTSSSKALVASERAFFEKKMVQQAYFPSEHKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANE6</accession>
    <name>G3ANE6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cofilin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Actin-depolymerizing factor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_61041</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. Binding to F-actin is regulated by tropomyosin. It is the major component of intranuclear and cytoplasmic actin rods. Required for accumulation of actin at the cell division site via depolymerizing actin at the cell ends. In association with myosin II has a role in the assembly of the contractile ring via severing actin filaments. Involved in the maintenance of the contractile ring once formed. In association with profilin and capping protein, has a role in the mitotic reorganization of the actin cytoskeleton.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the actin-binding proteins ADF family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375211.1">
        <property type="nucleotide sequence ID" value="XM_007375149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANE6"/>
    <dbReference type="FunCoup" id="G3ANE6">
        <property type="interactions" value="829"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANE6"/>
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    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61041"/>
    <dbReference type="eggNOG" id="KOG1735">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094004_3_2_1"/>
    <dbReference type="InParanoid" id="G3ANE6"/>
    <dbReference type="OMA" id="QCRFAVY"/>
    <dbReference type="OrthoDB" id="10249245at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11286">
        <property type="entry name" value="ADF_cofilin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cofilin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017904">
        <property type="entry name" value="ADF/Cofilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11913">
        <property type="entry name" value="COFILIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="11">
        <location>
            <begin position="5"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="141" mass="15736" checksum="F4231A2EC774B2AA" modified="2011-11-16" version="1">MSRSGVSVSDDALSTFNELKLGKKFKFIIFSLNDNKTEIVVESTSTDTDYDAFLEKLPENECKYAIYDFEYEIGGGEGKRSKIVFFTWSPDTAPVRSKMVYASSKDALRRALNGVAADVQGTDFSEVAYESVLEKVSRSTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANX2</accession>
    <name>G3ANX2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DASH complex subunit DAD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Outer kinetochore protein DAD1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_138437</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DAD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375873.1">
        <property type="nucleotide sequence ID" value="XM_007375811.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANX2"/>
    <dbReference type="FunCoup" id="G3ANX2">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANX2"/>
    <dbReference type="GeneID" id="18870118"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138437"/>
    <dbReference type="eggNOG" id="ENOG502SBWQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2321828_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANX2"/>
    <dbReference type="OMA" id="ENVSELW"/>
    <dbReference type="OrthoDB" id="5566853at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013958">
        <property type="entry name" value="DASH_Dad1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28025">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28025:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08649">
        <property type="entry name" value="DASH_Dad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="15" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW32597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="99" mass="11104" checksum="786EE146D1E6A673" modified="2011-11-16" version="1">MSRPTTSGNPLQPSTTSDFEKQRSLLIQQIANELSNLNTNIETLNRVFDQSLSIGKEFDDLGRLWSKFYAGVDELKQRREAQQRENVGPVTPGAIPPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP82</accession>
    <name>G3AP82_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61718</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MEMO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375929.1">
        <property type="nucleotide sequence ID" value="XM_007375867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP82"/>
    <dbReference type="FunCoup" id="G3AP82">
        <property type="interactions" value="514"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP82"/>
    <dbReference type="GeneID" id="18874020"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61718"/>
    <dbReference type="eggNOG" id="KOG3086">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038085_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP82"/>
    <dbReference type="OMA" id="MHLPYIH"/>
    <dbReference type="OrthoDB" id="417112at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07361">
        <property type="entry name" value="MEMO_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.830.10">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00055">
        <property type="entry name" value="MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002737">
        <property type="entry name" value="MEMO1_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04336">
        <property type="entry name" value="AmmeMemoSam_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060">
        <property type="entry name" value="PROTEIN MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060:SF0">
        <property type="entry name" value="PROTEIN MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01875">
        <property type="entry name" value="Memo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="341" mass="38788" checksum="8238C5521C78E4A7" modified="2011-11-16" version="1">MNVRSATHAGSWYTNSPTKLRAQLDSFFEKAKRNEKTIHGPIANDSIPGARILIGPHAGYAYSGERLAETFTAWDTSKVKRVFLLGPSHHVYFRNYALVSSFDSYATPFGEIPVDKETISKLLRKESKFDSRDAVFKLMSEEVDEDEHSFEMHAPFIHYRGEKAPLGMPKIIPIMISGMDEKLNEDIVECLLPYMDHEENHFIISSDFCHWGSRFSYTKYLNTEDTSLKTVGHDLYSLGTFNKPKELEIYKSIELLDRMALDIATKGSSRDWQEYIKITGNTICGQKPISIVLRLVEEYHKKAPRSSPVDGVFNWIGYSQSNPVRRSGESSVSYASGYVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP84</accession>
    <name>G3AP84_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_138093</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375931.1">
        <property type="nucleotide sequence ID" value="XM_007375869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP84"/>
    <dbReference type="FunCoup" id="G3AP84">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="GeneID" id="18870096"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138093"/>
    <dbReference type="eggNOG" id="KOG4150">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000809_1_0_1"/>
    <dbReference type="InParanoid" id="G3AP84"/>
    <dbReference type="OMA" id="CEWGCPL"/>
    <dbReference type="OrthoDB" id="18781at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000405">
        <property type="term" value="F:bubble DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901255">
        <property type="term" value="P:nucleotide-excision repair involved in interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17923">
        <property type="entry name" value="DEXHc_Hrq1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18797">
        <property type="entry name" value="SF2_C_Hrq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055227">
        <property type="entry name" value="HRQ1_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018973">
        <property type="entry name" value="MZB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957:SF3">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09369">
        <property type="entry name" value="MZB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22982">
        <property type="entry name" value="WHD_HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="316"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="567"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1139" mass="129136" checksum="876264C48A3534F8" modified="2011-11-16" version="1">MNQSSVSIPSEWPPFFTRLAQIFFQINTHLTFLSSHSRNIIPSIELLQKLNNTIIPLDLVIIKSIFPPGEIFFDYVDENQVQLSFIEEVKIDPYKGYQQTPIADKLPDEHEHHSKHILIFDFQDAKLHSIAAAVKGKKRVRYEQGVDFDHDQRREFFLSSSAYNLTPLTHSQLIHLINSRNGKFIELIKEYIDQFHGQSKYSLAEEKLLAKANKLIPEPPHLTDIIEDVQEKDEIIQESTMDLSSDEMIQALKDSRIYQDQIAIVQTLDQAKGPEYNQLSARVSDTIHPDLKQALLAAKGISIDEGLYTHQTMALENLLSNDSHVIVSTPTASGKSLIYQIPMLNAILWDIQSGLKTRYSTAFFIFPTKALAQDQLRHLRDFIKYIPNLKRPITVNAYDGDTPFKEREQIMKESDILFTNPDTIHASILPKNHYGPWKEFLTQLKFVVMDELHVYRGTFGINVSFVMGRLQRINHMLSPGDKPVRFVSCSATIQNPVSHFRAVCSLSQEDTVVHVAQDGSPHCEKKLVVWNPPLLMNKRGDKQKTDSNSPMAPRVGMIAESAKVLLSLLSAYKEIKAIVFCPIRVVCEMLMKEVRYLLQNTSFSKSGIDQSDILAYRGGYSKSDRRIIERKMFDGQLRAIIATNALELGIDLSDLDVVICCGFPMSKSNLHQQFGRAGRGHRTSGSLAIFVPGRNPLDQYYLDNPLELVDRTYEELCVQGLRNMDSSSLILERHLQCAAYEEAIDLQNDFKWILPGGSFKMYSQLVESHLLLDNDGKYKPHPSFLPKPHNLVQIRAVEEVQFAVVDITNNRNIIIEEVEASRTSFTLYEGGIFLHQGQQYLVKEFNHKDHYAKVERVNVDWTTSQRDFTDIDPEEVESVKPLYPMEHPQPLDIPAFFGKIKITMKVFGFFKVNRREEIIEAVEVKNPPVIMHAKGFWINVPRQVLKILEGKQLSAAGAVHASEHAIMNMLPIFINGSPDKGATIGTLELATECKAPTKEFSGRESSRKRPARLIFYDTKGGKLGSGISYKSYECIDAILHATFKRVKNCLCEWGCPLCIISSDCREQMKVISKPGAILVLGSILGKDLDILKDEVPDGPEFNMPDVNTDTIVESSSNVKFSPDVKILQVTRNRRCFTGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3APB4</accession>
    <name>G3APB4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_138491</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375368.1">
        <property type="nucleotide sequence ID" value="XM_007375306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APB4"/>
    <dbReference type="FunCoup" id="G3APB4">
        <property type="interactions" value="374"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APB4"/>
    <dbReference type="GeneID" id="18870122"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138491"/>
    <dbReference type="eggNOG" id="ENOG502SDEV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_9_1_1"/>
    <dbReference type="InParanoid" id="G3APB4"/>
    <dbReference type="OMA" id="THTSIFG"/>
    <dbReference type="OrthoDB" id="3998031at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06168">
        <property type="entry name" value="LSMD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034110">
        <property type="entry name" value="LSMD1_Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="1">
        <location>
            <begin position="4"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="91" mass="10150" checksum="AC9C5F2210DD4191" modified="2011-11-16" version="1">MSSIAPAQFIGANLHVRVVDSRILEGILTVIDPFGNLLLSNVYETSVDRLNNKNLHVREIGLVSVPRETIVSIKVDRKTHKNIFGTEQNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3APG3</accession>
    <name>G3APG3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_61752</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375956.1">
        <property type="nucleotide sequence ID" value="XM_007375894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APG3"/>
    <dbReference type="GeneID" id="18874034"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61752"/>
    <dbReference type="eggNOG" id="KOG0255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008455_11_0_1"/>
    <dbReference type="InParanoid" id="G3APG3"/>
    <dbReference type="OMA" id="IANCLMP"/>
    <dbReference type="OrthoDB" id="6770063at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF48">
        <property type="entry name" value="MULTIDRUG TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G02700)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="281"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="325"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="1"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="404" mass="44945" checksum="E528BAE0CCBD16C4" modified="2011-11-16" version="1">MSEEVGRKPIYVVTLAVALVFIVPCGAAQNIGTLIISRLIDGIAFSAPMTLIGGSLADIWEGPERGTAMAIFSAAPFLGPVCGPIFGGLLADHAPTWRWIYWTFLIIAGAFYAVFIVIVPETHHGILLKKRAKKLRKETGDSRYRTFSELQVRTFGQVAKTSLLRPFVLLSELIVFLMTMYMAVVYGLLYMFFFAYPMVFMEGKGFSASLTGVMFIPIGVGVIIATFCAPYFNKDYNKRAQKYRDRGELPPAELRLIPMMISCWFVPASLFAFAWSSYPTISWAGPCFAGLGAGFGFCCLYNPANNYIVDSYQHYAASALAAKTFVRSIWGACVPLFTIQMYHRLGYEWASSLMAFISLACCAIPFLFFKFGARIRSYSKYAYSPNLDAKQGDAENQKEQDFGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3APP0</accession>
    <name>G3APP0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_61293</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375487.1">
        <property type="nucleotide sequence ID" value="XM_007375425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APP0"/>
    <dbReference type="STRING" id="619300.G3APP0"/>
    <dbReference type="GeneID" id="18873824"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61293"/>
    <dbReference type="eggNOG" id="KOG1432">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1291753_0_0_1"/>
    <dbReference type="InParanoid" id="G3APP0"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="214" mass="24735" checksum="50BDC607D92B351D" modified="2011-11-16" version="1" fragment="single">MYMNRHYKKLIFSSLFLIALLSSVVYILQPKPFTPPDIQILDYPDEQDQGINQKIANVITDVRILKCNNYKCKVPGGYQQILPQLNYYTQKVSRTSMYSYYVIVKKQPIKSTTRAIVDLDIVPQDEDYEEVAGGTSELKLYKRSIVINMSKQIPKDLPLVRSIEALFGTNDLVDSREHHSTLHLSNDESIHPILSVFKLSQDKEKDFFEKKKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3APS8</accession>
    <name>G3APS8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LSM2-LSM8 complex subunit LSM8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LSM8</name>
        <name evidence="11" type="ORF">SPAPADRAFT_50817</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">LSm subunits form a heteromer with a doughnut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375525.1">
        <property type="nucleotide sequence ID" value="XM_007375463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APS8"/>
    <dbReference type="FunCoup" id="G3APS8">
        <property type="interactions" value="436"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APS8"/>
    <dbReference type="GeneID" id="18871489"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50817"/>
    <dbReference type="eggNOG" id="KOG1784">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_8_1_1"/>
    <dbReference type="InParanoid" id="G3APS8"/>
    <dbReference type="OMA" id="AACDQTT"/>
    <dbReference type="OrthoDB" id="422364at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01727">
        <property type="entry name" value="LSm8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000027">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034103">
        <property type="entry name" value="Lsm8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044642">
        <property type="entry name" value="PTHR15588"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588">
        <property type="entry name" value="LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588:SF9">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="101" mass="11328" checksum="6984706B9EF0EC33" modified="2011-11-16" version="1">MSDLKEFMGKKVHVITADARFFEGVLQGYDNSTNIILQDCIERILYSEEDVDEENQEIPLGLYIIRGGEVACVGEIDPAKYATIDWSTLKASTLKTTKNPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3APV1</accession>
    <name>G3APV1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_61356</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375548.1">
        <property type="nucleotide sequence ID" value="XM_007375486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APV1"/>
    <dbReference type="FunCoup" id="G3APV1">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APV1"/>
    <dbReference type="GeneID" id="18873855"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61356"/>
    <dbReference type="eggNOG" id="ENOG502S504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115063_1_0_1"/>
    <dbReference type="InParanoid" id="G3APV1"/>
    <dbReference type="OMA" id="LNAWSCV"/>
    <dbReference type="OrthoDB" id="67317at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056552">
        <property type="entry name" value="Ribonucl_Kappa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026770">
        <property type="entry name" value="RNase_K"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31733">
        <property type="entry name" value="RIBONUCLEASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23489">
        <property type="entry name" value="V-ATPase_su_f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW32272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="84" mass="9243" checksum="91E2EB2F6D10B7B3" modified="2011-11-16" version="1">MKPIVSSFQAYCCTILSAFAIVILSVIGYLFKTNSESFMGSINDPEDGEAVASTVFGAVFVYLAFFVFCGSQVWLIKRQGRIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3APW6</accession>
    <name>G3APW6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_153008</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375563.1">
        <property type="nucleotide sequence ID" value="XM_007375501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APW6"/>
    <dbReference type="FunCoup" id="G3APW6">
        <property type="interactions" value="148"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APW6"/>
    <dbReference type="GeneID" id="18870938"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_153008"/>
    <dbReference type="eggNOG" id="KOG4206">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041869_3_1_1"/>
    <dbReference type="InParanoid" id="G3APW6"/>
    <dbReference type="OMA" id="LFQDLNC"/>
    <dbReference type="OrthoDB" id="277802at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12246">
        <property type="entry name" value="RRM1_U1A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="31"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="120"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="135" mass="15523" checksum="92E2F26D73FCA65B" modified="2011-11-16" version="1">MPPKKRQQSSEKEANTSKKTKLDPSNLKPSQCLYIKNLNDKINRQLLKHNLYLLFSTFGDVIEIRIRKGFAHVLFGDIPSATVALRSLQSEEFFDKPLVINYSVNESKVITRLSGHIQEGGEDEDVPRYEEEEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ06</accession>
    <name>G3AQ06_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_61405</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375602.1">
        <property type="nucleotide sequence ID" value="XM_007375540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ06"/>
    <dbReference type="GeneID" id="18873876"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61405"/>
    <dbReference type="eggNOG" id="ENOG502S1AG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070630_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQ06"/>
    <dbReference type="OMA" id="VWLGPLN"/>
    <dbReference type="OrthoDB" id="4002732at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990444">
        <property type="term" value="F:F-box domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062026">
        <property type="term" value="P:negative regulation of SCF-dependent proteasomal ubiquitin-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070372">
        <property type="term" value="P:regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052449">
        <property type="entry name" value="STYX-Interacting_Phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46588">
        <property type="entry name" value="SERINE/THREONINE/TYROSINE-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46588:SF1">
        <property type="entry name" value="SERINE_THREONINE_TYROSINE-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="271" mass="31335" checksum="6B53644A5DA5F587" modified="2011-11-16" version="1">MFTSWQETMFFNPESPQEICPNIWLGPYSSLSGSFLVENPMQIIINCGTTLKFLDLVENDKSVAISSDVILLSLDPAFDSNHELVDKFKRQFTKILQHYLNFFYLENPKSALLMHKLPQNSIELTSPIISGSNLKLQFFNLIRLMTLFKSINPSLQILVVSDDGNSSLSTGLVIAYLMDCYRYNVANSFRLIQHRRPSVRELNSNFYDDLLIIEHLKKFYVENCEIKNCHTPIATTNYMLKRRNDECVEDEDDMVDEILVGGGDRKRRFVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ38</accession>
    <name>G3AQ38_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">40S ribosomal protein S19-A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_61954</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376163.1">
        <property type="nucleotide sequence ID" value="XM_007376101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ38"/>
    <dbReference type="FunCoup" id="G3AQ38">
        <property type="interactions" value="846"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ38"/>
    <dbReference type="GeneID" id="18874120"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61954"/>
    <dbReference type="eggNOG" id="KOG3411">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108559_3_0_1"/>
    <dbReference type="InParanoid" id="G3AQ38"/>
    <dbReference type="OMA" id="WAPFVKT"/>
    <dbReference type="OrthoDB" id="428974at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000118">
        <property type="entry name" value="40S ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001266">
        <property type="entry name" value="Ribosomal_eS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01090">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01413">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="16029" checksum="1272A7DC33EF97FB" modified="2011-11-16" version="1">MPGVSVRDVPAQDFINAYAQFLQRQGKLEVPGYVDIVKTSAGNELPPQEAETWFYKRAASVARHIYLRKQVGVGKLNKLYGGAINRGFRPHRHADASGSINRKVMQSLQKIGVLEISPKGGRRISENGQRDLDRIAAQTLEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ46</accession>
    <name>G3AQ46_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane sensor/transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_61960</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetate uptake transporter (AceTr) (TC 2.A.96) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376171.1">
        <property type="nucleotide sequence ID" value="XM_007376109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ46"/>
    <dbReference type="GeneID" id="18874123"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61960"/>
    <dbReference type="eggNOG" id="ENOG502QUJS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051062_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQ46"/>
    <dbReference type="OMA" id="WIEGPEP"/>
    <dbReference type="OrthoDB" id="3648309at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015123">
        <property type="term" value="F:acetate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051633">
        <property type="entry name" value="AceTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000791">
        <property type="entry name" value="Gpr1/Fun34/SatP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047622">
        <property type="entry name" value="GPR1_FUN34_YAAH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038013">
        <property type="entry name" value="AceTr_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123:SF1">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01184">
        <property type="entry name" value="Gpr1_Fun34_YaaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01114">
        <property type="entry name" value="GPR1_FUN34_YAAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="260" mass="27981" checksum="5C10BBB7501A2C3A" modified="2011-11-16" version="1">MASTSSQKSVNTSLEEHTPLGKVQIAGEGGEYVIINKNKYYRHDLMAAFGGNLNPGASPYPTININPAPLGLCGFALTTFVLSLYNAQAMGITIPNVVVSLACFYGGAAQFFAGCFEFVAGNTFGFTALTSYGAFWLSYAAIFIESFGILEGYADAEEQIPNAVGFFLIGWAIFTFILWLNTFKATVPFCALFFFLFVTFLLLAGGEFSGHVNVGRAGGVFGVITAFIAWWNAYAGVATPSNSYLQPIYIPMPGNQLKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ79</accession>
    <name>G3AQ79_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase</fullName>
            <ecNumber evidence="4 14">2.4.1.256</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ALG10</name>
        <name evidence="15" type="ORF">SPAPADRAFT_56279</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the third and last glucose residue from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide intermediate Glc(2)Man(9)GlcNAc(2)-PP-Dol to produce Glc(3)Man(9)GlcNAc(2)-PP-Dol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>an alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate = a alpha-D-Glc-(1-&gt;2)-alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29543"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="Rhea" id="RHEA-COMP:19512"/>
            <dbReference type="Rhea" id="RHEA-COMP:19522"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:132522"/>
            <dbReference type="ChEBI" id="CHEBI:132523"/>
            <dbReference type="EC" id="2.4.1.256"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:29544"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the ALG10 glucosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.256" evidence="4 14"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376205.1">
        <property type="nucleotide sequence ID" value="XM_007376143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ79"/>
    <dbReference type="FunCoup" id="G3AQ79">
        <property type="interactions" value="682"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ79"/>
    <dbReference type="GeneID" id="18872023"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56279"/>
    <dbReference type="eggNOG" id="KOG2642">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017053_1_0_1"/>
    <dbReference type="InParanoid" id="G3AQ79"/>
    <dbReference type="OMA" id="VWDSKIT"/>
    <dbReference type="OrthoDB" id="2305498at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106073">
        <property type="term" value="F:dolichyl pyrophosphate Glc2Man9GlcNAc2 alpha-1,2-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016900">
        <property type="entry name" value="Alg10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12989">
        <property type="entry name" value="ALPHA-1,2-GLUCOSYLTRANSFERASE ALG10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12989:SF10">
        <property type="entry name" value="DOL-P-GLC:GLC(2)MAN(9)GLCNAC(2)-PP-DOL ALPHA-1,2-GLUCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04922">
        <property type="entry name" value="DIE2_ALG10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028810">
        <property type="entry name" value="Alpha1_2_glucosyltferase_Alg10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="125"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="162"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="278"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="358"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="405"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR028810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW31427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="441" mass="51789" checksum="4781AAAC4C41EDC8" modified="2011-11-16" version="1">MALLIPSYVYIGIKSVFVRHLVSTEVQQPFIDEIFHLRQCQVYCNYNFTHWDNKITTPPGLYLLGFVYSKSLQFVIGGDCKDYNLLRSLNLVGGLVVFPLILHKFKQGSGSGSSFWTINLISQPLLFTYYFLFYTDVWSAILIVASLSLVSYKHKQYPLISAVLGFISLWFRQTNIVWLAFILAVLVDVKVKERTFMSRISKFTVEIVRSWLSVIPFVVNFILFVIFLKINGGITFGDKENHQMQFHVVQVFYCFAFINFFTWPVWLTTHRVRDYLKFIIGGYYGMNVVFNVISMFGIKFIIDKFTIVHPFLLADNRHYTFYIFKKILSHKYSSFVTIPVYHFSTYNIVSLLSRSRRLSPITVLAYLVSIALTIIPSPLFEPRYYIVPLLVFRLYIMPKKEYSHVIEFIWLNGINLITMYVFFNYEFTWDSEPGSIQRIIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQB2</accession>
    <name>G3AQB2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_139992</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376237.1">
        <property type="nucleotide sequence ID" value="XM_007376175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQB2"/>
    <dbReference type="FunCoup" id="G3AQB2">
        <property type="interactions" value="990"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQB2"/>
    <dbReference type="GeneID" id="18870260"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139992"/>
    <dbReference type="eggNOG" id="KOG2048">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002392_2_1_1"/>
    <dbReference type="InParanoid" id="G3AQB2"/>
    <dbReference type="OMA" id="STYITEW"/>
    <dbReference type="OrthoDB" id="8883818at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034455">
        <property type="term" value="C:t-UTP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000896">
        <property type="entry name" value="U3 small nucleolar RNA-associated protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046351">
        <property type="entry name" value="UTP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44163">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44163:SF1">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="205"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="735" mass="83193" checksum="C62C9BFAB631ABDB" modified="2011-11-16" version="1">MNIHRCRFVDYTPHTITATAFSHTSSLSKPPSTDLRLAVGRSNGDIEIWNPRYNWTHELTLPGSKDRSIEGLCWAMESEDNSESLRLFSIGGSTYITEWDLATGKPLTNYDCNAGIIWSIDISADNEKLAVGCDDGSVVIVDISGGKGSLEYDLICQRQDARVLSIKWVDNSTIIGGCADGRIRSWSASQETKGRIMATMRVDKSKTESTLVWSLTILPNKRQFASGDSTGSVKIWDLDHYSLLQSFKVHEADVLSLVHDVNQEKLFSAGVDRKIHQFDLTSTKSTSKWVHSFNRLLHSNDIRSMSIFENKGYSFLISGGVERAIVIQDVQSFHDGKYKKLLINQQKSNVIVNENKRLIFMWQDQTVKIWRASSEDSEKPKLVCKLSLSDDENITSIDFNEGSNLLAVSKMTSVTIFQLEESKSKLKVHKIRDENFVSLVEGAKLVKFISATKFLVLTSDEELYKFEINVEEEKIELEDEIELIANKQGKLPYAACIKNLIVTPNGDNLIISRFNGSIEIYPLGEGEAYVLTKLSNNPHLIACSDNDKLLVLSEDNKILEFFIKQDSTSLLTPWSRRNSEFLPQQFTTLEDKAQGMFVKDEKVWIFGSTWVAYFDLTMNIPIGKAYKNANNSKKRTRTGLSINEDEFENDMEESVDILETSLKQSEIDRLRQQIQDDEQQPNGSATSNRKPFFVTEKYRPIMKVADCGTNEIMVIERPYFALPSTPAFNLPKLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQE8</accession>
    <name>G3AQE8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_67550</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376273.1">
        <property type="nucleotide sequence ID" value="XM_007376211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQE8"/>
    <dbReference type="FunCoup" id="G3AQE8">
        <property type="interactions" value="105"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQE8"/>
    <dbReference type="GeneID" id="18875169"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67550"/>
    <dbReference type="eggNOG" id="KOG4283">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032951_2_1_1"/>
    <dbReference type="InParanoid" id="G3AQE8"/>
    <dbReference type="OMA" id="WIPAPRE"/>
    <dbReference type="OrthoDB" id="361494at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031464">
        <property type="term" value="C:Cul4A-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000109">
        <property type="term" value="C:nucleotide-excision repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042238">
        <property type="entry name" value="Rad28/ERCC8/Ckn1/ATCSA-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46202">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46202:SF1">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="42"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="118"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="204"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="269"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW31495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="450" mass="50761" checksum="BA70DAEC960A164C" modified="2011-11-16" version="1">MQSLLLDRKLGNITPLAFATTISESYYARLHQTAKHNVFPINCHHNASVNSLALDQSDYRFLLSGCADSSIKLWDLKQTHTQRQENEVDAALGNADYDEFDYDNPITTFTNVATIPKKSVHEFGISAVQWWPYDTGMFVSASFDHFVKIWDTNELTPVHDFDLESRIYAIDICRNETNSLVAAASDSPFIRLLDLRSASSSHTLSGHKGKTLSVKWHPINPHLLASGGYDGEVKVWDIRRSNSCLCRLDMLRTNIQNSSSSSNLTRESVKAHSGPVNGLVWDESGTELYTTGNDDKVRVWDMVSSLAPPVNKLINFGPLTRNKYPQTIPIMLNPKIETETRYLLFPSENSDIFIFRTIDGKLEARLSRKGSKNVGRTCSMVNAGPFTGTYYCGTMDGEIISWSPNWVKPDANDIIHTDQEMDLDLTDILQKRKQQAEERALLYSDPYFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQS3</accession>
    <name>G3AQS3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">T6SS Phospholipase effector Tle1-like catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_140598</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007376336.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144272_0_0_1"/>
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    <dbReference type="OrthoDB" id="3162439at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="Tle1-like_cat"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33840:SF2">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="T6SS_Tle1-like_cat"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T6SS Phospholipase effector Tle1-like catalytic" evidence="2">
        <location>
            <begin position="36"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="11"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="153" mass="17745" checksum="99581DB9B6442346" modified="2011-11-16" version="1">MTNQDTPIIHYKDKEYSLRDLNEKFHSLEKELNQTKSLILCLDGTENEFGPKPFTNVLKLFGMLEKDSDTQLCYYQPGIGTGFQAESDDLRQSNFISSKITHITNQIDSMIAFTVEKHVIAAYEFISRFYSNGDKIYLFGFRFVLHDTYMLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="6" type="ORF">SPAPADRAFT_140622</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
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    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007376374.1"/>
    </dbReference>
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    <dbReference type="STRING" id="619300.G3AQZ6"/>
    <dbReference type="GeneID" id="18870309"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140622"/>
    <dbReference type="eggNOG" id="KOG4206">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041869_1_1_1"/>
    <dbReference type="InParanoid" id="G3AQZ6"/>
    <dbReference type="OMA" id="LKKGWVM"/>
    <dbReference type="OrthoDB" id="4159781at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12246">
        <property type="entry name" value="RRM1_U1A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12247">
        <property type="entry name" value="RRM2_U1A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000039">
        <property type="entry name" value="U1 small nuclear ribonucleoprotein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="10"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="143"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="105"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="115"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW31658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="215" mass="24889" checksum="970A0A52435B4194" modified="2011-11-16" version="1">MASDSNQPLETIYINNLNDRVSLTKLKSELESLFTSYGKILQITAHRNLKMKGQAFITFESKVSSTKAMDLQNHILFNKPMHITYAKGNSDAYYELHNEVDKIEQRKSEKERQNLKRKSTPAGGKQKKKKVKSEDWKSLPPNKLLLIQRLDDDEQLTSYFEQFEGFSNVRLVKVRKLAFIEFDNVESSTKCLKSIKTEELQEKFGSEVILSFAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR39</accession>
    <name>G3AR39_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Anaphase spindle elongation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_56495</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376478.1">
        <property type="nucleotide sequence ID" value="XM_007376416.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="G3AR39">
        <property type="interactions" value="182"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR39"/>
    <dbReference type="GeneID" id="18872040"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56495"/>
    <dbReference type="eggNOG" id="KOG4302">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_320776_0_0_1"/>
    <dbReference type="InParanoid" id="G3AR39"/>
    <dbReference type="OMA" id="KRITRHF"/>
    <dbReference type="OrthoDB" id="642895at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051256">
        <property type="term" value="P:mitotic spindle midzone assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007145">
        <property type="entry name" value="MAP65_Ase1_PRC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321:SF41">
        <property type="entry name" value="FASCETTO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321">
        <property type="entry name" value="PROTEIN REGULATOR OF CYTOKINESIS 1 PRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03999">
        <property type="entry name" value="MAP65_ASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="685"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="813"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="689"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="714"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="726"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="813"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="830"/>
            <end position="839"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="894" mass="103310" checksum="2B1267AD834F28E2" modified="2011-11-16" version="1">MSLTNNHSKPTLDDPSPVKHTILEQFNSSHEEISDTSTPSQIESILGKPTGTDSVSSVTQRPPQSKVKMNPFAEMSAKRMQQIGSDVIQALSEVTDECELVGFTEPEISHRQEEIFTSIRDTIATSKAKIHQERANIEDECEWLRQQIQLILSMVNDNKGDKSLSLIERGLAFKNQDQYEDGYKEQVLTKFSDIRGNTNFHTSSPFNIADSSLLNSSMSIEQQYDNMLNNIPRLTLLQQKSQLNGIFLKVLKSFMISFRRLNNLNLEYTELIDLIGPFRTSSSNIVHILPSSTDAEYHKQILDEFESSIRHLNLQKEKNFTENENYNTFILGSPRKNSEQTSESVLDHLRNINYQIIRIIRGLRFTKFTSDFFSSVQVEIDHCKIEIDKRKESVARILNQILESLELLQITDDKFKEMQKHNENINDDMLLDIETLKTIQKEPLEFGLHNSHIEFLSGIQDLLASTVNTRKSQWDEYSKTCMQLWNKLGETEEYVNSFMERNSSLTELSLLSFKMELNKLYIRRSECIEKFIADARNEIEKLWDKMYYSRVLRSEFEFFNYDPDNSDVEKETVLAKHEEEIIKLNEEYAAKEPIFQLYEELLELLKDQDFLKEASKDSSRLMSKNSCKILLNEEKLRKKINKNMPRVIDSLVSSVYEYNNSDNNGSHPLAINGEDLYEKLLSIQTEQQSNGPRRNRNVSNSSRVSPTKKPVQRISPQRKVQPTSRPESRLKHARESPTKVTRIAKPQVKLARTYNNPTTIKISNAITSSFSSASSNSGSPTRILGNISALYPHTELQPLNRPLRPLDMLSEQNSPLRLNHSPNQKLDSPCLEKEQDKENSSPFHLSPINIKSNFFERPKDIDRRLSTNTCDTSAMNGDEYQNWREERIRQYHGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR73</accession>
    <name>G3AR73_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_61825</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376026.1">
        <property type="nucleotide sequence ID" value="XM_007375964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR73"/>
    <dbReference type="STRING" id="619300.G3AR73"/>
    <dbReference type="GeneID" id="18874069"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61825"/>
    <dbReference type="eggNOG" id="KOG0880">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_4_1_1"/>
    <dbReference type="InParanoid" id="G3AR73"/>
    <dbReference type="OrthoDB" id="271386at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000025">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase CYP19-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0697">Rotamase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442573" description="peptidylprolyl isomerase" evidence="6">
        <location>
            <begin position="19"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="272"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="7">
        <location>
            <begin position="77"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="309" mass="35116" checksum="D8242C9A98CA5F0D" modified="2011-11-16" version="1">MRVTILQTIFIFISIALSAVIPPEEQNAPNELLVGEINPEEEPDFLSIKELEYLKGDPDVTHKVSFTISQRVSDSESKKLGKLTIALFGKTVPLTVENFYQLSTRTLKHSYQDVLFHRIIKGFMVQGGEIKTEKGESCSIYGGNFKDENFELKHDKLGRVSMANSSPDRNGCQFFILNVGETPHLDGHHVVFGQLVDGFDTLETISNVNVGKQNKPKNDIYVSDIEAIKRSEEMKEPLDEIEQAHKDQTNQEFEFGDEKKVAQQEPPVSEGYSYFIIMLLVGVILVGVLYYQLIHRRKKITAIRQDKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR98</accession>
    <name>G3AR98_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Golgi mannosyltransferase complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_62310</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ANP1/MMN9/VAN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376483.1">
        <property type="nucleotide sequence ID" value="XM_007376421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR98"/>
    <dbReference type="FunCoup" id="G3AR98">
        <property type="interactions" value="128"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR98"/>
    <dbReference type="GeneID" id="18874265"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62310"/>
    <dbReference type="eggNOG" id="ENOG502QRPX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017872_4_0_1"/>
    <dbReference type="InParanoid" id="G3AR98"/>
    <dbReference type="OMA" id="IPKTREG"/>
    <dbReference type="OrthoDB" id="2405412at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140497">
        <property type="term" value="C:mannan polymerase II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000017">
        <property type="entry name" value="Mannan polymerase II complex ANP1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052086">
        <property type="entry name" value="Mannan_Polymerase_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083:SF6">
        <property type="entry name" value="MANNAN POLYMERASE COMPLEXES SUBUNIT MNN9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083">
        <property type="entry name" value="MANNAN POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03452">
        <property type="entry name" value="Anp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="42143" checksum="1BCF12178F653553" modified="2011-11-16" version="1">MLYYKNPYFNYVRRRPLALVGPLAVLIMIYLFFFSSSGKKDTAKYSYNKKSSAWFGKNKDSVILRNLPKNHISHYDLNKLTISPDALTKKEEVLILSPMSKFLPEYWENLNKLTYDHSLISLGFIFPRTQEGDQALKELEDSLKRSKAQNKLNFKKITLLRQDSNSLKSQAEKDRHAFKVQKERRSMMALARNSLVFTTISPTTSWVLWLDADIVETPPTLIQDLTAHNKPVLSANVYQRYFDPETNQKSIRPYDFNNWVESEEGLKIASGLADDEIIVEGYAEMATYRPLMAHFYDANGDVNSEMALDGVGGGAVMVKADVHRDGAMFPSFPFYHLIETEGFAKMAKRLGYEVFGLPNYLVYHHNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARC6</accession>
    <name>G3ARC6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_51715</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376511.1">
        <property type="nucleotide sequence ID" value="XM_007376449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARC6"/>
    <dbReference type="FunCoup" id="G3ARC6">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="GeneID" id="18871584"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51715"/>
    <dbReference type="eggNOG" id="KOG1153">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011263_1_0_1"/>
    <dbReference type="InParanoid" id="G3ARC6"/>
    <dbReference type="OMA" id="FASHSAW"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04077">
        <property type="entry name" value="Peptidases_S8_PCSK9_ProteinaseK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000007">
        <property type="entry name" value="Subtilisin-like serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034193">
        <property type="entry name" value="PCSK9_ProteinaseK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF13">
        <property type="entry name" value="SUBTILASE-TYPE PROTEINASE RRT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442324" description="Peptidase S8/S53 domain-containing protein" evidence="7">
        <location>
            <begin position="18"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="8">
        <location>
            <begin position="135"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="401" mass="43661" checksum="764C6B245E123A15" modified="2011-11-16" version="1">MLLDLILLLLILQCCLGEPYLVSLKLEKTFESFYAYDTKFPPTERVRDLITNTYSFDDFNAFSGNFPKNIIERLEKCEFVAEISPDAKFKVAVIKSQPNAPKHLARVNRRTPMKKEKKGEKKKKYPYIYDSAFSGQNVNVYVIDSGIQHEHPEFTGRARMGKDFTGEGGDDLLDHGSYVAGIIGSATYGISKSCNLINVKVVDKNDQADASTIIAAIEFIVKHHKKGNNKGVVNLSIGVDANRLINRAVKVAKRMGLVVVAAAGNDNVDACTKSPAGSPHAITVGAINDVDDSIASFSNWGKCVDFFAIGTNVASVNPKDDTTPEIKKGTSMSAPVVTGLVANLLSKGIDPGSVKHHLLSIAIKGKIPDSRDFDKGTRNIIAHSGIFLNDLPKYIPVHPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARI6</accession>
    <name>G3ARI6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_61877</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376085.1">
        <property type="nucleotide sequence ID" value="XM_007376023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARI6"/>
    <dbReference type="STRING" id="619300.G3ARI6"/>
    <dbReference type="GeneID" id="18874091"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61877"/>
    <dbReference type="eggNOG" id="KOG2451">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_0_1"/>
    <dbReference type="InParanoid" id="G3ARI6"/>
    <dbReference type="OMA" id="NWYGFNV"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050740">
        <property type="entry name" value="Aldehyde_DH_Superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353:SF6">
        <property type="entry name" value="CYTOPLASMIC ALDEHYDE DEHYDROGENASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="4">
        <location>
            <begin position="50"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW31307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="486" mass="52813" checksum="76EBC9299CB91E27" modified="2011-11-16" version="1">MTRPPVRSISNLVPNIISGKEVASAKLQIPVLSPNAPYETLHHYTSFPVSPESISELSSIVQSGFKTWSNTSETERIKIFTRAIELLQQRRHELIESQLEIGMPMWFAGFNVDGFIGQVQEYINMLGNSSSTSIIPSAETDLAMAVRSPIGPVLSISPWNAPVLLAGRSIMAPLAAGCSVIVKPAEKSPKCAYLLVKCFLDAGVPGNVLQLVNVKPQENAVFIDKLLGTGVIRKLNFTGSTLVGKSIAATAGSHLVPCLLELGGKNASIVCPDADIDQAVGKITWSGWSHKGQICMSTDVAYIHESVYDEFKSKLVTVAREIAQDPDYLIPQRDLVGTTKVVNLIEDALNKGASLTFGEFSKQEIESSGIIKPIILENVIKGMELYDQESFGPVFSIEKYSNINQVIDKINHSPYGLKSAIWSRNVFEAIKLAKQIETGGVHINNSTVHDEPTVPHGGLKSSGIGRFNSRWGIDDFTVVKSITVNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARJ2</accession>
    <name>G3ARJ2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_154279</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376091.1">
        <property type="nucleotide sequence ID" value="XM_007376029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARJ2"/>
    <dbReference type="FunCoup" id="G3ARJ2">
        <property type="interactions" value="378"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARJ2"/>
    <dbReference type="GeneID" id="18870975"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_154279"/>
    <dbReference type="eggNOG" id="KOG0585">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015561_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARJ2"/>
    <dbReference type="OMA" id="MKECNHP"/>
    <dbReference type="OrthoDB" id="68483at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14008">
        <property type="entry name" value="STKc_LKB1_CaMKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF72">
        <property type="entry name" value="SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="25"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="466"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="466"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="478"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="516"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="560"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW31313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="589" mass="67300" checksum="91D4E0AD99A0B384" modified="2011-11-16" version="1">MPPTTTRLTIDLDPKSHKKVINNQYKIVKKIGQGQYGKVLLGQSTTNGDYVAIKTINRIDKSRLLTKNYVSPITKIKREIQIMHECNHPNVVKLYQVIDDTKFDKILLILEYCKFGEIDWKNYNHYYEKYTSKRDPPLTLNKILRDVVLGLEYLHFKRIIHRDLKPSNLLISSDNTIKISDFGVSVIFENEQDMSELGKTMGTPAFYGPELCSFINNRYSMINNSNHQLNKVIDYRMDIWSLGVTIYALMFNDLPFSGNNEFEMCKNILTQELKFPRVRQSSKCTASDMQELISFKNLCKCLLAKDPNDRMTLAQVKLHSFTTFDLNDNEKYKYRKFNNPYIHQHSQGLPSRIKKFFSKQKTTKPTLPSVPASSINASSSSVDRANISLEHVDDLLDSYLDDSSSMGSVEEEDFDTTDILAEIHSPSPIVKPLPPQLDLTSSAHSFIIGESSPSSAYFSPMKRYFNNNNNNNNNNNNESSPAVSDSKPTISSLTRDFGELDLIPPPSMHKSTTPKLSPSRKNSISSSSSSLNLNGYLTADDYSPRSHYKRGSRDEKKEEPEEEEEDTLIFDESMIRNYNGMSDYLDNID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARN0</accession>
    <name>G3ARN0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATPase expression protein 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_62380</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for translation of the mitochondrial OLI1 transcript coding for the mitochondrial ATP synthase subunit 9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Binds to the 5'UTR of the OLI1 mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AEP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376561.1">
        <property type="nucleotide sequence ID" value="XM_007376499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARN0"/>
    <dbReference type="GeneID" id="18874297"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62380"/>
    <dbReference type="eggNOG" id="ENOG502SDKY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_671152_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARN0"/>
    <dbReference type="OMA" id="KWFQRFE"/>
    <dbReference type="OrthoDB" id="4077974at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024319">
        <property type="entry name" value="ATPase_expression_mit"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12921">
        <property type="entry name" value="ATP13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="410" mass="48397" checksum="040A58A12194C6FC" modified="2011-11-16" version="1">MNRKLDLAAKWFRTFKRESPYDYVGKMTPKMWILVFQIYSGGNLHLWELKSTELASIRYDPKNSRFVDSIKSNDLLADFRKSGLQVAEMGLEFHETLICSLGYEGKLSQLFNFIESVWGVDETGKIISDDVKLKPNDSRYPTMNTVISIFSSIAYNGHFFDAMKFVSEFQKSYEITGSNALDKRFFDSIFSWCDMSTKFEEDRALSYFLRQTNVKTNGTPTLNEISNDVNFDYEGFLEFVKKLKQERNQAFQQLWDIYQTDEIDFNHSIYSTYLNYLKEGIADNLEQKYYDYLSALLRQYQAYHTTSQSFNRRANLGFEPVNEKHQSVYILYQAAMKELIDIKGKSLYIGQCQPLIDEWSLDTAMKQQLSDWFKHEKLNQFKRLREKKRQEFMMTLKKEDDEDDSLLSLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARP9</accession>
    <name>G3ARP9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoglycerate mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62395</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376580.1">
        <property type="nucleotide sequence ID" value="XM_007376518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARP9"/>
    <dbReference type="FunCoup" id="G3ARP9">
        <property type="interactions" value="174"/>
    </dbReference>
    <dbReference type="GeneID" id="18874307"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62395"/>
    <dbReference type="eggNOG" id="KOG4754">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039184_0_2_1"/>
    <dbReference type="InParanoid" id="G3ARP9"/>
    <dbReference type="OMA" id="RETFDCH"/>
    <dbReference type="OrthoDB" id="496981at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF1">
        <property type="entry name" value="HISTIDINE PHOSPHATASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="321" mass="36710" checksum="19454AF67D274081" modified="2011-11-16" version="1">MSTSKSNPNPLDVLDSKINPDNVKDYQTRLESYHSSLDSYWKFEIVPGIFKQSDPSTDERKFDYLKEHFGIIGSWDDIIENLNHLNNSTGPNEQYKIMFLARHGQGFHNLAHTKYGDNAWNEYWSKLNGDGEIIWGPDANLTELGISQAKDNNYIWKQEQINNKNNDASLIIPTKFLVSPLSRSIDTMYYTWNDIVDLKNVQPLIQENWRETMGVHTCDKRSSRTIIDYKYTGKGFVIEPGFAEEDIYYQDDYRETVGEQALRINKGLQQLFNESPNDKIVAITSHSGSIRAQLLAVGHRSFAVGTGGMIPVFVKAIKVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS36</accession>
    <name>G3AS36_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62490</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TTI2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376662.1">
        <property type="nucleotide sequence ID" value="XM_007376600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS36"/>
    <dbReference type="GeneID" id="18874346"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62490"/>
    <dbReference type="eggNOG" id="ENOG502S9D8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_604333_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS36"/>
    <dbReference type="OMA" id="AWFLIDY"/>
    <dbReference type="OrthoDB" id="6417021at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110078">
        <property type="term" value="C:TTT Hsp90 cochaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018870">
        <property type="entry name" value="Tti2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10521">
        <property type="entry name" value="Tti2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="453" mass="51814" checksum="399333CEF38708F2" modified="2011-11-16" version="1">MATTSRLTSEQIETALAGVLDIKDAISSISTQLDVLTDESTLKLEALLTKCPTDISDELQRHLKVDKTYKRSEEYYHYLRLLRVSKYHTDLQLIYTLSLHSNPNVPWCNNINSQIVTSIITEINMPNEIIEDFIENEYKRNIQQVKSERSSSGLRPRLGFNSVASEISSSEQIEKLKAYPISLSQAWFLIEISTDKHRWCSQVFNLTISFILNLLDLSDIEIKTHACQLLQFLLSKSSHFKFNSGLIKEVSKSLSKCLVYIPPSVSVDRSLPILSQAYPCLIEVYSTYGTNLDYVELISTIISNVNHLLTSEVEITEPVLVLLLDQVLVMSEKLDTDITICLSKLLYTLNQVIVNINICERVPQVIAKALNCQSRIITIFQSSTSEFKQILYSYRFDLLGAYGILYKRIKHFKDEAQLIDCMTENITQFGELSQDIDTYTEWQDLTKLVIPIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS92</accession>
    <name>G3AS92_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_52233</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377084.1">
        <property type="nucleotide sequence ID" value="XM_007377022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS92"/>
    <dbReference type="GeneID" id="18871650"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52233"/>
    <dbReference type="HOGENOM" id="CLU_1240795_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS92"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="223" mass="25033" checksum="47534F6649614A22" modified="2011-11-16" version="1">MGEGKDVLRNNYTDLSDTTLFASLSIGPSIPFNQIIPPEGLQIDEMSIPKVSQDNYDNNTPTLMMSGGSQDNEIDVYVTTRQFQFWESTSNSPHDIQDDALSSCNAEFASCLELSPAISTPVHNSLKRQSSNMNILAEVNKRKRSGQCDSNESLTPLSFNQELFDSVFSEAELFEQGDSIYKPNIESFTNVEYLNKLSRESSTRIQEFANCLAYRLSKYTDET</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASA3</accession>
    <name>G3ASA3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MEP2</name>
        <name evidence="11" type="ORF">SPAPADRAFT_62504</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376676.1">
        <property type="nucleotide sequence ID" value="XM_007376614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASA3"/>
    <dbReference type="FunCoup" id="G3ASA3">
        <property type="interactions" value="308"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASA3"/>
    <dbReference type="GeneID" id="18874354"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62504"/>
    <dbReference type="eggNOG" id="KOG0682">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_33_5_1"/>
    <dbReference type="InParanoid" id="G3ASA3"/>
    <dbReference type="OMA" id="GFPTTPM"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019740">
        <property type="term" value="P:nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900430">
        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029:SF10">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="257"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="10">
        <location>
            <begin position="1"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="335"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="359"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="373" mass="40311" checksum="1523737421628E2C" modified="2011-11-16" version="1">MFAATTAILMVGAGCERARLGPMMVFLFLWLTVVYCPIAYWTWGGNGWLVKLGALDFAGGGPVHENSGFAALAYSLWLGKRHDPLTQGKVPKYKPHSVSSIVLGTVFLWFGWFGFNGGSTGNASIRSWYAAVNTNIAAASGGLTWMFVDYFRTGGKWSTVGLCTGAIAGLVGITPAAGFVPVYTSIIFGIVPAIICNFAVDLKNILQIDDGMDVWALHGVGGFVGSFMTGLFAADYVGMIDGTEIAGGWINHHWKQLGYQLAGSVSIGAWSFTVTSILLLVMDRIPQLRIRLHEDEEMLGTDLAQIGEFAYYDEEDSEHDAYVLEPIRSTPAIKAKLKASTTRPEEEEPTPIEGKTVDGQDSLNQNDSDVSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASC0</accession>
    <name>G3ASC0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Ornithine aminotransferase</fullName>
            <ecNumber evidence="4 9">2.6.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_62527</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 2-oxocarboxylate + L-ornithine = L-glutamate 5-semialdehyde + an L-alpha-amino acid</text>
            <dbReference type="Rhea" id="RHEA:13877"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="EC" id="2.6.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-ornithine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.13" evidence="4 9"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376693.1">
        <property type="nucleotide sequence ID" value="XM_007376631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASC0"/>
    <dbReference type="FunCoup" id="G3ASC0">
        <property type="interactions" value="836"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASC0"/>
    <dbReference type="GeneID" id="18874361"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62527"/>
    <dbReference type="eggNOG" id="KOG1402">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_3_1"/>
    <dbReference type="InParanoid" id="G3ASC0"/>
    <dbReference type="OMA" id="RSAWDLC"/>
    <dbReference type="OrthoDB" id="10261433at2759"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00358"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004587">
        <property type="term" value="F:ornithine aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019544">
        <property type="term" value="P:L-arginine catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010121">
        <property type="term" value="P:L-arginine catabolic process to proline via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006591">
        <property type="term" value="P:ornithine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000011">
        <property type="entry name" value="Ornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000152">
        <property type="entry name" value="Ornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010164">
        <property type="entry name" value="Orn_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01885">
        <property type="entry name" value="Orn_aminotrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF18">
        <property type="entry name" value="ORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="435" mass="47407" checksum="40EDA7FDAB8F6647" modified="2011-11-16" version="1">MTPPNFNVSSETARQYESEYSAHNYHPLPVVFSHAKGAHVWDPEGNEYLDFLSAYSAVNQGHCHPKIVEALVDQASKLTLCSRAFSSDVFGVFAKYITEYFNYEMVLPMNTGAEAVETGLKLARRWGYVKKGIAPNEAIILSATNNFHGRTLGVISMSTDPDARTSFGPYLPNVGPQIPGEPEGTFIRYGVIEDLELAVKNAGDKVAAILLEPIQGEAGIVVPPEDYLPRVQELCKKHNILLICDEIQTGIARTGKMLCYEHSQGVKPDIILLGKAISGGVMPVSAVLSSKEIMLTLEPGSHGSTYGGNPLACRVAIAALEVVKDENLVERSQKLGDFLKQELVKLQGESDGMISEVRGKGLLSAIVIDEAKTNGRTAWDLCLIMKKYGVLAKPTHDNIIRLAPPLIISEEDLLKGVDSIRKAVKELPTAEKTEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASE9</accession>
    <name>G3ASE9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 11">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7 11">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_142267</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 10 11">Belongs to the peptidase C12 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7 11"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377100.1">
        <property type="nucleotide sequence ID" value="XM_007377038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASE9"/>
    <dbReference type="STRING" id="619300.G3ASE9"/>
    <dbReference type="MEROPS" id="C12.009"/>
    <dbReference type="GeneID" id="18870436"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142267"/>
    <dbReference type="eggNOG" id="KOG2778">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018316_1_0_1"/>
    <dbReference type="InParanoid" id="G3ASE9"/>
    <dbReference type="OMA" id="YIQYEIQ"/>
    <dbReference type="OrthoDB" id="1924260at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019784">
        <property type="term" value="F:deNEDDylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071629">
        <property type="term" value="P:cytoplasm protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09617">
        <property type="entry name" value="Peptidase_C12_UCH37_BAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.532.10">
        <property type="entry name" value="Peptidase C12, ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001578">
        <property type="entry name" value="Peptidase_C12_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036959">
        <property type="entry name" value="Peptidase_C12_UCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017390">
        <property type="entry name" value="Ubiquitinyl_hydrolase_UCH37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041507">
        <property type="entry name" value="UCH_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589:SF16">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE ISOZYME L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01088">
        <property type="entry name" value="Peptidase_C12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18031">
        <property type="entry name" value="UCH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038120">
        <property type="entry name" value="Ubiquitinyl_hydrolase_UCH37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00707">
        <property type="entry name" value="UBCTHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52048">
        <property type="entry name" value="UCH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7 10" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 10" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UCH catalytic" evidence="12">
        <location>
            <begin position="5"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8 10">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8 10">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="site" description="Important for enzyme activity" evidence="9 10">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR038120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR038120-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR038120-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="315" mass="36091" checksum="662AF107514699AA" modified="2011-11-16" version="1">MSEGGWNTIDSDAGVFTELVEKLGVQDVEFNDLYVIDTDSLATLQPVHGVIFLFKYGKLYRQYASDGNKPLDGTYDNDYLEHDIFFAQQTINNACATQAALNVLFNVDGIDLGEELSNFRSFVTGFDSFMIGETISNSDLIRSVHNSFSTPSMFVDEDKPQPPSDYESDSLFHFVGYVYKSGRIYELDGLKSYPIIHEECSSQQEFIEKLPGVIQRRIAKHGENELRFSLLAVTNNKLEQARTIGDESEVHHQLHKREVWSHENELRKHDYTGLIVELLKNISKNKTDEEWEELLKKGRKHTQQLIQQRIAKSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASI2</accession>
    <name>G3ASI2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_63027</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNR ribonuclease family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377133.1">
        <property type="nucleotide sequence ID" value="XM_007377071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASI2"/>
    <dbReference type="FunCoup" id="G3ASI2">
        <property type="interactions" value="239"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASI2"/>
    <dbReference type="GeneID" id="18874559"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63027"/>
    <dbReference type="eggNOG" id="KOG2102">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002333_0_3_1"/>
    <dbReference type="InParanoid" id="G3ASI2"/>
    <dbReference type="OMA" id="FDINRYH"/>
    <dbReference type="OrthoDB" id="372421at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048027">
        <property type="term" value="F:mRNA 5'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000900">
        <property type="term" value="F:mRNA regulatory element binding translation repressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903450">
        <property type="term" value="P:regulation of G1 to G0 transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.700:FF:000002">
        <property type="entry name" value="Cell wall biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000100">
        <property type="entry name" value="Cell wall biogenesis protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041505">
        <property type="entry name" value="Dis3_CSD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041093">
        <property type="entry name" value="Dis3l2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF9">
        <property type="entry name" value="DIS3-LIKE EXONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17877">
        <property type="entry name" value="Dis3l2_C_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17849">
        <property type="entry name" value="OB_Dis3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNB" evidence="3">
        <location>
            <begin position="190"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="703"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="703"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="790" mass="88688" checksum="6A58B1BEEDC79CE7" modified="2011-11-16" version="1">MKKNDDVEVEGQSLLLVEEEEINDEIKPLYAGHVVAVVDRIPGQLFAGTLGLLRPAQAAQAARDKKNGKESSVHTPKSPKIVWFKPTDKKVPLIAIPTEQAPKDFVENHEQYADQLFVASIKRWPITSLHPFGTLVSKLGKIEDPSTEIDSILRDNNFLCDEYPEEDEEEITVNMYDLPLPDPEFENRARTEYLNDYIIAFSQNGSFVDHALHVKRLSSTKIELGFHAADVSYFIKPGSILERKSKKRSSSVFLPQKMVSLYPRHVQQMISFTENERNLAVSVIFEIDTLNFEVEDLYIHESVIVPKQVVNYDSFDKVLVGGEQGEISSATCDYIKTFSLIAKEFRRQRLDNRELGISPNLTLLDQLDDVRVRLSLNIFNDSLAFDVISEISHKVNTAIAAKIHAGLGDHALLRRQALPTLQKMETFVRKATNLGYKMDTSTSASLQNSILKIEDATKRQCVETLLYKCMSRGRYYVAGKQDPDSYGHYYLNLPLYAHFTAPLRRYADLIVHRQLKAVLNKVEYDIDLDNLKAVADYCNFKEDCASQAQEQAIHLLVSQTINEVSESAGQLLCMGTVIQVYESSFDVFIPEFGVEKRVHGDQLPLVKAEFDKLDRVLELYWEKGVDSATYIPPDEQTSLSYRNSIKNKHRASAIHAAKIQSKTILEKNNVITDSIAEKLAELKLEPPKLSIPTLKNGENVTTATKSMPSTPVLSGNGGSDEFPKSLRTNSSSVITGDSGLAPYLQDTITRVEGDSCIQVIKELTQVPVLIRAEIGMALPCLTVRVLNPFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASR6</accession>
    <name>G3ASR6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alcohol dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ADH1</name>
        <name evidence="10" type="ORF">SPAPADRAFT_68326</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377163.1">
        <property type="nucleotide sequence ID" value="XM_007377101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASR6"/>
    <dbReference type="FunCoup" id="G3ASR6">
        <property type="interactions" value="992"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASR6"/>
    <dbReference type="GeneID" id="18875210"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68326"/>
    <dbReference type="eggNOG" id="KOG0023">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_20_1_1"/>
    <dbReference type="InParanoid" id="G3ASR6"/>
    <dbReference type="OMA" id="YKGLKMT"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08297">
        <property type="entry name" value="CAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000002">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF3">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="9">
        <location>
            <begin position="16"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="348" mass="36619" checksum="8D20A6A7CE562BAF" modified="2011-11-16" version="1">MSVPKTQKAVIFETNGGPLQYKDIPVPVPKPNEILINVKYSGVCHTDLHAWKGDWPLPTKLPLVGGHEGAGVVVALGENVKGWKIGDLAGIKWLNGSCLSCEFCQETNEPNCPNADLSGYTHDGSFQQYATADAVQAARIPAGTDLAHVAPILCAGITVYKAIKTADRKQGDWICVSGAGGGLGSLAIQYAKAMGYRVIGIDGGADKGEFVKSLGAEAFIDFTKEKDVVAAVIKATDGGPHASINVSVSEAAIQQSVDYTRSTGTVVLVGLPAGAKVVAPVFDSVVKSLSIKGSYVGNRADSQRAIEFFSRGAIKCPIKVVGLSELPEVYKLMEEGKILGRYVVDTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT30</accession>
    <name>G3AT30_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ACT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_62651</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetolactate synthase small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376826.1">
        <property type="nucleotide sequence ID" value="XM_007376764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT30"/>
    <dbReference type="FunCoup" id="G3AT30">
        <property type="interactions" value="338"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AT30"/>
    <dbReference type="GeneID" id="18874420"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62651"/>
    <dbReference type="eggNOG" id="KOG2663">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055003_0_0_1"/>
    <dbReference type="InParanoid" id="G3AT30"/>
    <dbReference type="OMA" id="CARSGMM"/>
    <dbReference type="OrthoDB" id="2013116at2759"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990610">
        <property type="term" value="F:acetolactate synthase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04878">
        <property type="entry name" value="ACT_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000001">
        <property type="entry name" value="Acetolactate synthase, small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="entry name" value="ACT-like. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004789">
        <property type="entry name" value="Acetalactate_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019455">
        <property type="entry name" value="Acetolactate_synth_ssu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039557">
        <property type="entry name" value="AHAS_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054480">
        <property type="entry name" value="AHAS_small-like_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053050">
        <property type="entry name" value="ALS_regulatory_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00119">
        <property type="entry name" value="acolac_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31242">
        <property type="entry name" value="ACETOLACTATE SYNTHASE SMALL SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31242:SF2">
        <property type="entry name" value="ACETOLACTATE SYNTHASE SMALL SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22629">
        <property type="entry name" value="ACT_AHAS_ss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10369">
        <property type="entry name" value="ALS_ss_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="7">
        <location>
            <begin position="71"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="295" mass="32451" checksum="C144AA7597B1DA73" modified="2011-11-16" version="1">MLKRSANVFRTSTRTRSSSSTSALAYKTLHRNQKRPPLPTIDTPSWSADTAVSSILYETPVPSKAPKKQHVLNCLVQNEPGVLSSVSSTLAARGFNIDSLVVCNTEVKDLSRMTIVLKGQDGVIEQARRQIEDLVPVYAVLDYTNAEIIRRELLLARVSLLGPEYFQELIATHQLHVDDASSIPDLTACESAYHPSNLPPSEALRQKHSHFNHISALTEQFGGKVVDISERNVVVELSAKPSRVSSFITLLQPFGILELARSGMMALPRTPLERHEEDEEPVAADIVDASQLPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT63</accession>
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        <submittedName>
            <fullName evidence="4">N-terminal acetyltransferase</fullName>
        </submittedName>
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        <name evidence="4" type="ORF">SPAPADRAFT_142846</name>
    </gene>
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        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Saccharomycotina</taxon>
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        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EGW30826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031416">
        <property type="term" value="C:NatB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:protein-N-terminal amino-acid acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="2"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="195" mass="22838" checksum="B0CFD035BDB5B8FA" modified="2011-11-16" version="1">MTSIKPFQIEDLFEFNSVNLDPLTENFNISFYLQYLTDWPQLFFKSVELSRHFGQPHEISGYMMGKTEGKLAKKEWHTHITAVTVQDQYRRLGLASELCLELEKMTSVAPYETLFVDLFVKVTNTLGRQLYEKLGYSVYRRVVGYYGQTMPMDRNAIDDEIDAFDMRKSLPRDTNNETIRDKGDKVYVLPQEVTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT68</accession>
    <name>G3AT68_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">40S ribosomal protein S23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_56791</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
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        <property type="protein sequence ID" value="EGW30831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="interactions" value="841"/>
    </dbReference>
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    <dbReference type="GeneID" id="18872068"/>
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    <dbReference type="eggNOG" id="KOG1749">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="OMA" id="KFRWSQR"/>
    <dbReference type="OrthoDB" id="1713912at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03367">
        <property type="entry name" value="Ribosomal_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="40S ribosomal protein S23"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_uS12_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003254">
        <property type="entry name" value="PRK04211.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="145" mass="15982" checksum="EADDAF189E6881E6" modified="2011-11-16" version="1">MGKGKPRGLNSARKLRVHRRNNRWADQSYKARLLGTAFKSSPFGGSSHAKGIVLEKIGIESKQPNSAIRKCVRVQLIKNGKKVTAFVPNDGCLNFVDENDEVLLAGFGRRGKAKGDIPGVRFKVVKVSGVSLLALWKEKKEKPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT98</accession>
    <name>G3AT98_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">RNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_156210</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376894.1">
        <property type="nucleotide sequence ID" value="XM_007376832.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT98"/>
    <dbReference type="FunCoup" id="G3AT98">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AT98"/>
    <dbReference type="GeneID" id="18871046"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_156210"/>
    <dbReference type="eggNOG" id="KOG4198">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022834_1_0_1"/>
    <dbReference type="InParanoid" id="G3AT98"/>
    <dbReference type="OMA" id="DWKCGEN"/>
    <dbReference type="OrthoDB" id="448399at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
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    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
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        <location>
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            <end position="350"/>
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    <sequence length="730" mass="81227" checksum="95B2D528B1D1284F" modified="2011-11-16" version="1">MSDIYVVLHLVTTCDDDNSTSSYVTKDSTELIELAWAVIDAETLEATYRDSILVRPVNTPITPYCSQLYRISWEHVRNAGTFKDAISKFDTYIQENVIAKDKEFSLVTFDLNKLRVALPREARDKGVVLPPYLQHPRVFDLPNEYSKWQTSHPEALSYTASSLANIVTALEVEVENIDEYGPMSSSASTPPPPPPPPPSSSTTTSSDQQPAPSQPVSGDVETKSKSTVDLYAKLVSQLIKKSMPLEDHPTVFTKPYDSAQDVRVFLSERSKILYLSNLPQDTTQSELESWFTHFGGRPVAFWTLKNLDPNVKKGINGFAVFATHEEATESLSMNGRVLSDRAIEVQPSSTRVLDKANDLLTPFPPSKNRPRPGDWTCPSCGFSNFQRRTHCFRCSFPASSAAAIQESIYSNPNGNNRRGSSQQAHAQPHTGDNKPVVNTGNVLQNYSGYPDQQQSQQPQQSQQSQQHQNYHQGGHNSNNNQYHNTNGSNANTGNNPSRSHYNNNVPFRAGDWKCELCMYHNFAKNLCCLKCGTTKPVMLNQQHQQQQQQQSQQQQAGASIHSVNSTATAIAAATASGQPLNSGFMGIQQPQPLHTYGHVSNQQQQQQQQQQQQQQQQQRQNRNSNSMGYTNQKYYSQGVRSSGTGTPNQMMSQQMMMYQQQQQHSGGANTQQKYSQSVNNSPSLYPSYYNNSNYQQGGGSQVPQNEGDASLNSSGYSSLSNQMNSLTLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATC7</accession>
    <name>G3ATC7_SPAPN</name>
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        <recommendedName>
            <fullName evidence="8">Transcriptional adapter 2</fullName>
        </recommendedName>
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        <name evidence="15" type="ORF">SPAPADRAFT_62780</name>
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        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="12">
        <location>
            <begin position="5"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="14">
        <location>
            <begin position="63"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="11">
        <location>
            <begin position="68"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="SWIRM" evidence="13">
        <location>
            <begin position="364"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="340"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR025024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW30890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="452" mass="51950" checksum="CA2775FD78F9124C" modified="2011-11-16" version="1">MDVKGRLFHCDVCSTDCSNRIRIKCAICNDYDLCVPCFAAGLTTGDHKPWHDYQIIEQNTYPIFERDWGADEELLLIQGCETFGLGNWADIADHIGNRSKEEVAAHYYKIYLESKDYPLPEMNKDFHDVTPIHFLEERKERLALRKSQPLPPPKGKPVPSVPLCHEIQGYMPGRLEFDHEAENEAEVPIKDMIFDPEDSINDIELKLTILDIYNSRLTTRAERKRVMILNNLLDYRKNISADKRKSKEEKDLLKRIDAYIRILSPEDFDTFTRDFLTELKCRIRIQQLQTWRRNGITTIEDGNKFEKDKLIRTAHYQRMGNGTLSSRHSATPTVMNGSISASNGYGSGRKPYSSANSPAPEYKPKISNNRAPLDISHAADFDLLSNEEKQLCSTLRILPKPYLAIKNQLMKEAVKNNGILKKKDARQALKIDVNKASKIYEFFVQMGWCSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATM2</accession>
    <name>G3ATM2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_62888</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FMP52 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377019.1">
        <property type="nucleotide sequence ID" value="XM_007376957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATM2"/>
    <dbReference type="FunCoup" id="G3ATM2">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATM2"/>
    <dbReference type="GeneID" id="18874512"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62888"/>
    <dbReference type="eggNOG" id="KOG4039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071330_2_2_1"/>
    <dbReference type="InParanoid" id="G3ATM2"/>
    <dbReference type="OMA" id="LGRTEWP"/>
    <dbReference type="OrthoDB" id="545683at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051170">
        <property type="term" value="P:import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000366">
        <property type="entry name" value="Protein FMP52, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014843">
        <property type="entry name" value="Him1/Fmp52"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14097">
        <property type="entry name" value="OXIDOREDUCTASE HTATIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14097:SF7">
        <property type="entry name" value="OXIDOREDUCTASE HTATIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08732">
        <property type="entry name" value="HIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="24883" checksum="DD3DBF7CE8C14A6C" modified="2011-11-16" version="1">MTSAFILGSTGLVGLQILKHSATVFAKVTTVSRRQPQASFPELDAIVEPDTSKWPSVISESDSTVYFSAFGTNRAAAGSGEKFKEIDYGINYECAKAAKQKGMKTCVLISSTGASASSPFLYFQTKGQLEKDIIDLKFERTIILRPGMLLGERDKPKGLFNDFLVATLKYTHGNFLSRFSYAIYGEELGMIAVSLANEEYDASNGPIVKILGGFELTELAKKLHNNNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU35</accession>
    <name>G3AU35_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_142985</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377383.1">
        <property type="nucleotide sequence ID" value="XM_007377321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU35"/>
    <dbReference type="GeneID" id="18870507"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142985"/>
    <dbReference type="eggNOG" id="ENOG502T61U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2687580_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU35"/>
    <dbReference type="OMA" id="VWMNNIT"/>
    <dbReference type="OrthoDB" id="5328412at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442572" evidence="1">
        <location>
            <begin position="23"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="70" mass="8105" checksum="CD499C819ED19092" modified="2011-11-16" version="1">MKIILWFIYLIKINLLQMIVHGSRNPSLTIQYIDSSIFEASRCTPESENYSADRAWANNLTDVPVMPEFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUL2</accession>
    <name>G3AUL2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Dipeptidyl peptidase 3</fullName>
            <ecNumber evidence="4 15">3.4.14.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 15">Dipeptidyl aminopeptidase III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14 15">Dipeptidyl peptidase III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_143827</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>Release of an N-terminal dipeptide from a peptide comprising four or more residues, with broad specificity. Also acts on dipeptidyl 2-naphthylamides.</text>
            <dbReference type="EC" id="3.4.14.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 17">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15 17">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the peptidase M49 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.4" evidence="4 15"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377539.1">
        <property type="nucleotide sequence ID" value="XM_007377477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUL2"/>
    <dbReference type="FunCoup" id="G3AUL2">
        <property type="interactions" value="654"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUL2"/>
    <dbReference type="GeneID" id="18870574"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143827"/>
    <dbReference type="eggNOG" id="KOG3675">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011977_1_0_1"/>
    <dbReference type="InParanoid" id="G3AUL2"/>
    <dbReference type="OMA" id="QRYWIRD"/>
    <dbReference type="OrthoDB" id="4694525at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.30:FF:000001">
        <property type="entry name" value="Dipeptidyl peptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.30:FF:000002">
        <property type="entry name" value="Dipeptidyl peptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.30">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005317">
        <property type="entry name" value="Dipeptidyl-peptase3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039461">
        <property type="entry name" value="Peptidase_M49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422:SF11">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE III-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03571">
        <property type="entry name" value="Peptidase_M49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007828">
        <property type="entry name" value="Dipeptidyl-peptidase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 15 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8 15" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15 17" id="KW-0862">Zinc</keyword>
    <feature type="active site" evidence="16">
        <location>
            <position position="445"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="449"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="495"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR007828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR007828-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR007828-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW30568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="688" mass="77992" checksum="A70A6B29627659FB" modified="2011-11-16" version="1">MSAISDFYADTEGPIVFLSAKKHFDALTTTNAKLYAHHLSRASFYGTRAVLRSVSPESEDIYDLILLIHELLNTPKTNEEYVKLLGDKVSEQTITYYLEYASQFLGNVGNYKSFGDKKFIPRLSPQEFDEFVKAVGEEKVSEKYNKVREAIFSVDQPLLGYPESGHLSNYYPDSPKITKTEIDAVNKALAKAGIMPENTRVVKNDHEFQVYVASAVGDNTTDYYPKELATEDGAKITFKFGDHASEFVHIVKHLEEALKYVANDTQAAMVKYYIESFKTGSMNAHKKSQIEWVKDIKPEVESNIGFIETYRDPSGVRGEWEGLVAMVNHERTAKFSQLVNNAGFFIKELPWDKIYEKDEFTPPDFTSLEVLTFAGSGIPAGINIPNYDDVRLNYGFKNVSLGNVLSANPKNPKKKEEITFIDESLQELFKKWRDDSFEVQVGLHELLGHGTGKLLQETSPGKFNFDKSDKRITSWYGPNDTWGSLFGLTAGSFEECRAELVALYLILKKPLEVLPIFDIKTPEDQKSVKFIATLLMVRAGLLGLEFWDPQSKKWGQPHMQARFGIFKQLHKAGVFSLDYSDDSFDDLKITLDESTLDNKAVDALGDFLSHLHIYKTTANVKEGVQYYVDKTTVDPEYARFRDVVLKKKQPRRKIIQGNTFINASGEVEVEEYEESEAGMINSYSDRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUM8</accession>
    <name>G3AUM8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_63423</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the p23/wos2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377555.1">
        <property type="nucleotide sequence ID" value="XM_007377493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUM8"/>
    <dbReference type="STRING" id="619300.G3AUM8"/>
    <dbReference type="GeneID" id="18874743"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63423"/>
    <dbReference type="eggNOG" id="KOG3158">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078883_3_1_1"/>
    <dbReference type="InParanoid" id="G3AUM8"/>
    <dbReference type="OMA" id="ARNYIYL"/>
    <dbReference type="OrthoDB" id="1564555at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06465">
        <property type="entry name" value="p23_hB-ind1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045250">
        <property type="entry name" value="p23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22932:SF1">
        <property type="entry name" value="CO-CHAPERONE PROTEIN DAF-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22932">
        <property type="entry name" value="TELOMERASE-BINDING PROTEIN P23 HSP90 CO-CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="2">
        <location>
            <begin position="3"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="104" mass="12072" checksum="D0B8996274AF4390" modified="2011-11-16" version="1" fragment="single">MSTQTPSVLWAQRSSADEASKNILYVTIEVLDPIDVKYDLTSSNLKFEANSSDKKIHYNLNIDFFDEVDPENSHVNVTGSHYFMVIRKKTAKEEYWPRLTKEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUQ5</accession>
    <name>G3AUQ5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="15">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="15">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ALA1</name>
        <name evidence="17" type="ORF">SPAPADRAFT_52679</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 15">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>tRNA(Ala) + L-alanine + ATP = L-alanyl-tRNA(Ala) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Alax-L subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="15"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377582.1">
        <property type="nucleotide sequence ID" value="XM_007377520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUQ5"/>
    <dbReference type="FunCoup" id="G3AUQ5">
        <property type="interactions" value="1024"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUQ5"/>
    <dbReference type="GeneID" id="18871705"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52679"/>
    <dbReference type="eggNOG" id="KOG0188">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004485_5_0_1"/>
    <dbReference type="InParanoid" id="G3AUQ5"/>
    <dbReference type="OMA" id="NKKDNFW"/>
    <dbReference type="OrthoDB" id="2423964at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070143">
        <property type="term" value="P:mitochondrial alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00673">
        <property type="entry name" value="AlaRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Alanine--tRNA ligase"/>
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        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_B">
        <property type="entry name" value="Ala_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
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        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050058">
        <property type="entry name" value="Ala-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059090">
        <property type="entry name" value="ALA1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023033">
        <property type="entry name" value="Ala_tRNA_ligase_euk/bac"/>
    </dbReference>
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        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777:SF9">
        <property type="entry name" value="ALANINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777">
        <property type="entry name" value="ALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ALA1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
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        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)"/>
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    </dbReference>
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        <property type="entry name" value="ThrRS/AlaRS common domain"/>
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    </dbReference>
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        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15 17" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 15" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 15" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="7 15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alanyl-transfer RNA synthetases family profile" evidence="16">
        <location>
            <begin position="14"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="607"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="611"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
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    <feature type="binding site" evidence="15">
        <location>
            <position position="726"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="730"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW30611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="959" mass="106717" checksum="045F9EDD9E08191F" modified="2011-11-16" version="1">MSTNTTNSVVANEWTASKVRSTFLDFFTKKKGHTYVPSSSVVPHNDPTLLFANAGMNQYKPIFLGTVDPASDFASLKRAANSQKCIRAGGKHNDLEDVGRDSYHHTFFEMLGNWSFGDYFKKEAIDWSWELLTQVYGLDKDRLYVTYFGGDEKQGLQPDLEAKQFWLNVGVAEDHILPGSAKDNFWEMGDQGPCGPCSEIHYDRIGGGRNAADLVNMDDPNVLEVWNVVFIQYNREPDSSLRTLPNKHIDTGMGFERLVSILQDKSSNYDTDVFGPIFDKITQITGVRPYAGRFGAEDKDGIDTAYRVIADHVRTLTFAICDGGVPNNEGRGYVLRRILRRGSRYVRKYMNYPIGSFFQQLVDVVIEQNKEIFPEIVHGASDLKEILNEEEVSFAKTLDRGEKLFEQYAIIASKTPEQTLSGKDVWRLYDTYGFPVDLTRLMAEEAGLKIDEPGFERAKEESREASKGTGTKNGTTLIKLDVHALSSLETELVPKTDDEYKYGTENIEAKIVSIYDGAEFVTKINDSTKQYGILLDKTPFYAEQGGQEYDTGKLVIDGKSEFTVDNVQVYGGYVLHTGTITEGELSVGDSIIASYDELRRWPIRNNHTGTHVLNYALREVLGDSVDQKGSLVAPEKLRFDFSHKQALSTAELEKVEAISNKYIKDNKTVYYQDVSLSQAREINGLRAVFGETYPDPVRVVSIGVPVDTLLSNPKNPEWHEVSIEFCGGTHVGETGEIKDLVVIEESGIAKGIRRIVAVTGHDARNVQRLASEFDQELDLADKLPFGQDKEARAKELGVRLKQLSISVLEKQKLNAKFNKLDKSIKDNLKAIQKEESKKTLDVVKQWLDQEKPADFLVSHVPINANAKAITEAFNLIKKQDKTKSVYLITGTSGKVAHGCYISDEAIAKGVDATVLAKAVSDKIGGKAGGKGNIVQGMGDKPEGINTAVEEVTKLLAEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUR2</accession>
    <name>G3AUR2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fimbrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_63448</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377589.1">
        <property type="nucleotide sequence ID" value="XM_007377527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUR2"/>
    <dbReference type="FunCoup" id="G3AUR2">
        <property type="interactions" value="627"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUR2"/>
    <dbReference type="GeneID" id="18874756"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63448"/>
    <dbReference type="eggNOG" id="KOG0046">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015284_3_0_1"/>
    <dbReference type="InParanoid" id="G3AUR2"/>
    <dbReference type="OMA" id="WQLMRKN"/>
    <dbReference type="OrthoDB" id="431378at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099079">
        <property type="term" value="C:actin body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120106">
        <property type="term" value="C:mitotic actomyosin contractile ring, distal actin filament layer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051639">
        <property type="term" value="P:actin filament network formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044837">
        <property type="term" value="P:actomyosin contractile ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070649">
        <property type="term" value="P:formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd21294">
        <property type="entry name" value="CH_FIMB_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21300">
        <property type="entry name" value="CH_FIMB_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fimbrin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000010">
        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000016">
        <property type="entry name" value="Probable fimbrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000027">
        <property type="entry name" value="Probable fimbrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039959">
        <property type="entry name" value="Fimbrin/Plastin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961:SF18">
        <property type="entry name" value="FI19014P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961">
        <property type="entry name" value="FIMBRIN/PLASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="51"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="134"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="281"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="405"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="526"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="90"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00073963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW30618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="638" mass="71098" checksum="C5E1C4EC2C6AA4B4" modified="2011-11-16" version="1">MNVLKLQKKFPNLQQSDLFTIIDDFRAIDLEDKGYVEKQDVYNSISKQGVYTYDQARETLKKVDVDASGHVELDDYVELVAKLKESTLAPEAPAPGSVSPRISKKQPPPVPTASSKNKTFITGKSEGTTHTINDDERIEFTRHINSVLAGDADIGKRLPIDPNTFQIFDECRDGLVLSKLINDSVPDTIDTRVLNLPKAKKLNNFQMSENANIVINSAKAIGCIVVNVHSEDIIDGKEHLILGLIWQIIRRGLLSKVDIKYHPELYRLLEDDETLEQFLRLPPEQILLRWFNYHLKNAGSNNRVSNFSKDIADGENYTILLNQLVPEQCDLSPLKESNLIARAEKILDHADRIGCRKYLTASSLVAGNPKLNLAFVANLFNNYPGLQPIEPHEQIEIEEFDAEGEREARVFTLWLNSLDVEPPVISLFEDLKDGLVLLQAYDKVLPGSVSFKHVNKKPANGEVSRFKALENTNYSVEIGKANGFSLVGIEGSDIVDGNKLLTLGLVWQLMRRNIINTLSELGKGANLSDSDILKWANAQVAKGGKSSKIRSFKDESLSTGEFLLDVLNGLKPGYVDYDLVHKGTNLSDDEKYANAKLAISIARKLGALIWLVPEDINEVRSRLILSFVGSLMAVAETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUX2</accession>
    <name>G3AUX2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_63681</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377829.1">
        <property type="nucleotide sequence ID" value="XM_007377767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUX2"/>
    <dbReference type="GeneID" id="18874866"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63681"/>
    <dbReference type="eggNOG" id="ENOG502R2HZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1327114_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUX2"/>
    <dbReference type="OMA" id="HIHFINY"/>
    <dbReference type="OrthoDB" id="4023344at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="207" mass="23832" checksum="CCFEA2FF8C4F78E9" modified="2011-11-16" version="1">MLQQLQSPFTYTAPEHIPNHNNNLLLPTPTFSEKSSDSTVYCLQTPISPIKKQLTMFENNLSMIHSKVSMMEDCALKREIFQLIQNSVHILQEVEDNDTLPQRTFDEFIIHTPPSSFEETFITPPPSSNQEFCYQTPRDKIIDYENCAPPAPPKQPNFDHAITNLNCSNLPNIIFSEEDLLADGTVQFNQHFLDWDSFEFDSQDLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUY0</accession>
    <name>G3AUY0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RNA polymerase II assembly factor Rtp1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_144558</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Tango6 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377837.1">
        <property type="nucleotide sequence ID" value="XM_007377775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUY0"/>
    <dbReference type="FunCoup" id="G3AUY0">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="GeneID" id="18870643"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144558"/>
    <dbReference type="eggNOG" id="KOG4653">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006300_1_0_1"/>
    <dbReference type="InParanoid" id="G3AUY0"/>
    <dbReference type="OMA" id="QVATLIC"/>
    <dbReference type="OrthoDB" id="39591at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019451">
        <property type="entry name" value="Rtp1_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019414">
        <property type="entry name" value="Rtp1_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039600">
        <property type="entry name" value="TANGO6/Rtp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20959">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 6 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20959:SF1">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10363">
        <property type="entry name" value="RTP1_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10304">
        <property type="entry name" value="RTP1_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA polymerase II assembly factor Rtp1 C-terminal" evidence="4">
        <location>
            <begin position="663"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase II assembly factor Rtp1 C-terminal" evidence="3">
        <location>
            <begin position="870"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="573"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="578"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="907" mass="102509" checksum="8685AB134A7F12AC" modified="2011-11-16" version="1">MSPPKIEELPKKNPFASRKKTTIKRDAKEVYPRQGLNKPDFVPSTPLDVLFAQLEKLLDLPYDKLSLNSLYSTIFPNDTTAESSLDQRYRVIEYLLDCLINIQKYSMESPKDKNLVSISLHDIKTFSKLINVIIIHGIYPCISPFNIGIPFNKRRLKQFTKDNITIDKLASNNEQLLMLIYTKLFQVFSQPSDVADLLTKGTGYSDFLTISIALITQFNHQEVLDKFDQIEAIPSTYELYSTYSLLLATPGSPRLFAQFVMAHLQQLVYTRPDGLLSLIEFVLGLRDNEDIQIEKFDQVADVVLQKPKTIATKPYFTSIGQQAYDLLVNINRPVVTSCVSYVIDKLWIRNKLVVHDFFLSHLHDAFNPKDGVTSEAQLNNAVNVLISLTKKSPALDLYSEIVGSILVPLWGYFTFLKKHEKPSEVVSGLLTSYFTVVETGLDDISKNLVTDGTDSWKFSLGPNSMTEIVPRNAEFENKSKEAKVNQFINDLDFAIDCFVILLQDLTDELVNSLFVSVLKRWLSSDDALEQRNPFIMLVDLRVLESVGNKFKDILARTPKEMLEIVASFLQSHPVTKQEDEDSDDEEEEEESDETTSLLLQLLSAILTETTNFSDCQSLLDQISKSLTRLNTSQSLALASRIANLRTSSLQPLSQDESDKLVLTRAIASLNDPLVPIRAHGLYLLRQLIQAHSPIITVDFVINLHLVQLKDPEPFIYLNVIKGLEAMIEFDDSVVTSLCELYGAGEDIDERLRIGEVLLRRVQQGGVMPEPIVVTALRLIERREVVVDDRIRMSAMSILGSCCRVNPLGMLGHLEQALDCALGILDLESDEDKAIMRRSAVVLIHDLVLGTSESDKVPFPEKYRERVIVKLRYVSETDSDLLVREQATAVLDTIDELVKLAMELYEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV94</accession>
    <name>G3AV94_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_52750</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377663.1">
        <property type="nucleotide sequence ID" value="XM_007377601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV94"/>
    <dbReference type="STRING" id="619300.G3AV94"/>
    <dbReference type="GeneID" id="18871718"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52750"/>
    <dbReference type="eggNOG" id="ENOG502RQ2Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_525786_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV94"/>
    <dbReference type="OrthoDB" id="4022079at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW29897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="552" mass="63610" checksum="5E51B06D2598FB4E" modified="2011-11-16" version="1">MLQIRLPHLKSPQANLDDSRHTKAIFMSPQLDHLNMSVLPSQIIEKAIQFLSHLDQTASSSTNILSPLINYYDTCNHSGSDTSHIIPIIARVTWKYIFHSRLDISSVIYHCEWLKRFTIDVGDHMKFSDWAMAMNATSTGSDTDEHNDLVVSKSFLGLVGAYLGKFMSITNVQISGERVKEVEDGINKLLELAFQDRMSREFTRRFSGLYPYIGEYVSQRQDSVPCKVMHQIINELSMTIEEAFKIVLQTLDHGITTHLDFRLVDKLRVAIETIHQNDLEVILQAFNKLICKTSLVMTLNYLNYILSYKLSNRNPNLVLPVTITRQFECFVLPPISKLEVQDPKEEIEKVFEQMYESEIPENLSVIQFRTLQLLARFLPQEAPTIEHTSQVKIASLTSKIVEQFNGMVISSLISALLVDSNNKYKQEVVFSTFHTLLSVKYTTKSNKIWITLINAVNDICYADLRYIPNFITLFEYLMKKDATIVDDELISGGLTFFIDTFDPEYKWFSKRETPVQLNYEIKMEDYKFLYGDAPTTPVKQGRQQSIHVDQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADZ9</accession>
    <name>G3ADZ9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_57787</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transcriptional coactivator that stimulates GCN4-dependent transcriptional activity by bridging the DNA-binding region of GCN4 and TBP (SPT15), thereby recruiting TBP to GCN4-bound promoters. Involved in induction of the ribosome quality control (RQC) pathway; a pathway that degrades nascent peptide chains during problematic translation. Required to prevent stalled ribosomes from frameshifting.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MBF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372134.1">
        <property type="nucleotide sequence ID" value="XM_007372072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ADZ9"/>
    <dbReference type="FunCoup" id="G3ADZ9">
        <property type="interactions" value="784"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ADZ9"/>
    <dbReference type="GeneID" id="18872197"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57787"/>
    <dbReference type="eggNOG" id="KOG3398">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112609_0_1_1"/>
    <dbReference type="InParanoid" id="G3ADZ9"/>
    <dbReference type="OMA" id="GKNKSCK"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000015">
        <property type="entry name" value="Endothelial differentiation-related factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013729">
        <property type="entry name" value="MBF1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10245:SF15">
        <property type="entry name" value="ENDOTHELIAL DIFFERENTIATION-RELATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10245">
        <property type="entry name" value="ENDOTHELIAL DIFFERENTIATION-RELATED FACTOR 1 MULTIPROTEIN BRIDGING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08523">
        <property type="entry name" value="MBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="7">
        <location>
            <begin position="85"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="150" mass="16202" checksum="6F2D7899E7AE68C5" modified="2011-11-16" version="1">MSDWDNVTIIGQKARVGGGGPRETVARTQGQLNAARRAGAVVGIEKKYGTANTKSNPEGQRLTKLDATDDVVTVKKLDASVGKAIQQARQEKKLTQKDLATKVNEKPNVINDYEAGRAVPNQQLLGKLERALGVKLRGKNIGEPLFAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE23</accession>
    <name>G3AE23_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">IMD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_58786</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372969.1">
        <property type="nucleotide sequence ID" value="XM_007372907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE23"/>
    <dbReference type="FunCoup" id="G3AE23">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AE23"/>
    <dbReference type="GeneID" id="18872606"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58786"/>
    <dbReference type="eggNOG" id="ENOG502QTA6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034174_0_0_1"/>
    <dbReference type="InParanoid" id="G3AE23"/>
    <dbReference type="OrthoDB" id="5594612at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013606">
        <property type="entry name" value="I-BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037470">
        <property type="entry name" value="IVY1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38407">
        <property type="entry name" value="PROTEIN IVY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38407:SF1">
        <property type="entry name" value="PROTEIN IVY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08397">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMD" evidence="3">
        <location>
            <begin position="44"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="270"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="145"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="326" mass="36769" checksum="A3D72C3D7FBDC107" modified="2011-11-16" version="1">MNSDNAAPASRQTSLNTFTSFSPSVLDMSSNDIITTTDFKHITNTYEELFKTATNLQAALEAVSAAANQFGQALEQCISECPKVNNTTMVEDGISNAAGLQYIMASNHQILGRMMHTSFIEPLQQELIKLKVEYGQNHSYYQQEIKSKSKLLKEHEAENLKLRKKKTRDLNKYKNNLLSLTNQLDDIDRVKYEYYHEINALVAGYNREHLLTRTGGLVRAELEIYESIAKKGWSGGGLDQLLSVSPDLFEINGEAGVEDIIDEATLELTERDDQQEQEEVEEAQEEGKNETVEDPDESFSLPIVSTTNTLLRKLHKDNKIDTIQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE77</accession>
    <name>G3AE77_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="9">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_48591</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">ATP-dependent DNA helicase involved in DNA damage repair by homologous recombination and in genome maintenance. Capable of unwinding D-loops. Plays a role in limiting crossover recombinants during mitotic DNA double-strand break (DSB) repair. Component of a FANCM-MHF complex which promotes gene conversion at blocked replication forks, probably by reversal of the stalled fork.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with the MHF histone-fold complex to form the FANCM-MHF complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the DEAD box helicase family. DEAH subfamily. FANCM sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="9"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373023.1">
        <property type="nucleotide sequence ID" value="XM_007372961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE77"/>
    <dbReference type="FunCoup" id="G3AE77">
        <property type="interactions" value="236"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AE77"/>
    <dbReference type="GeneID" id="18871242"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48591"/>
    <dbReference type="eggNOG" id="KOG0354">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002513_1_1_1"/>
    <dbReference type="InParanoid" id="G3AE77"/>
    <dbReference type="OMA" id="TIAHINT"/>
    <dbReference type="OrthoDB" id="164902at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033677">
        <property type="term" value="F:DNA/RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070336">
        <property type="term" value="F:flap-structured DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033567">
        <property type="term" value="P:DNA replication, Okazaki fragment processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007535">
        <property type="term" value="P:donor selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045003">
        <property type="term" value="P:double-strand break repair via synthesis-dependent strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060543">
        <property type="term" value="P:negative regulation of strand invasion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18033">
        <property type="entry name" value="DEXDc_FANCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12091">
        <property type="entry name" value="FANCM_ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18801">
        <property type="entry name" value="SF2_C_FANCM_Hef"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000861">
        <property type="entry name" value="Fanconi anemia, complementation group M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
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        <property type="entry name" value="FANCM/Mph1-like_ID"/>
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    </dbReference>
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        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025">
        <property type="entry name" value="FANCONI ANEMIA GROUP M FANCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025:SF20">
        <property type="entry name" value="FANCONI ANEMIA GROUP M PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="93"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="424"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="481"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="747"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="481"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="491"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="508"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="770"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="781"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="829"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="852"/>
            <end position="867"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW35611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1151" mass="130038" checksum="B7D7A622B810F49F" modified="2011-11-16" version="1">MPVIPIHDEEDDDDWAFDDEEEDPELQQILQNGVSSKRQRTLDGNIVPTITSVQQLPKPIPVNIPSHHPIDYENLKTYIYPTNFQIRDYQYNIVQRAFYDNLLVALPTGLGKTFIASTVMLNFLRWFPQSKIIFMAPTRPLVAQQIKACCSITGIPSSKVAILLDKTRKNREEIWDTRQVFFTTPQVVENDLARGILNPKSVVLLVIDEAHRARGNYSYNNVVKFLSRFNNSFRILALTATPAGDVEGVQDIIDNLKISKVEVRTEHAIDITKYMKRKQIVKRTVNASSEIQELIGLLATAISPVLKSANERGVFDITDPTRINHFQCMEASRKVTMNRTMPSGLKWSNFYMLQLLGVVGQCYRRLNIYGVRSFYTYFNEKYNEFKGKKSKSQINSDFYFSDEIKQVLKRSDEVLKGKGYSHPKIETLMDELTDFFADGVSSDSRVIIFTEYRESALEIVQCIERTETNLKPHIFIGQSKEKERFDETKSKTKGKSKKRAHDGDDDVRDSTRTSSEDAQIKGMNQKLQKEIIKKFKAGAYNILVATSIGEEGLDIGEVDLIICYDSTSSPIKNIQRMGRTGRKRDGKVVLLFSSNEELKFDKAMAGYEYIQQHIQKGDLITLCSRNRIIPDIYTPKVVEQFIEIPDENIELKSEDDEDEIIRIATQYMLGGSQKKKKSTKKPLASKKKFFMPDNVETGFQSVTNMIKKSGDEVEKKRERDILDSFLDSSDDDDLPIIANPSLVRSSTSSRKLKVVDSDSNDDIPISSTSRQHSNPQASDSPNKRQVIDLKSDEDIPIASKAVSKSDSSKKKQVSSDSDNELSIHKDLNTPASSLVSEFPVSSKSESGSIPIDLDDDFSFSSDDESEVSESTREPTPLVPSSPKPKPKSLGVKRAVVVPNDHTASPPPVTSIPVEKAKMLTKISESNFNIPSSFNPHSEIGRPKKTLGLKRTRVNILDQLKNRQSQLAATHEDINRDHSGTRSAPIVLDDIDLPNYTPDSQVCVKHELEQDQTNDYSIDVESEPDKSTISVHDSGHDTVVINQSRVNKHYDIEQKSHNSSIYEFSSDSEGLLTEDQRMQLYTNYYVPVDPSQTVQFYDPFDGLKGKKKNHGKIPHSLNTEKLIDYLNRGILEKPGEDGTDDSSFDLSSIVYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEX3</accession>
    <name>G3AEX3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein MSF1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59015</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373215.1">
        <property type="nucleotide sequence ID" value="XM_007373153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEX3"/>
    <dbReference type="FunCoup" id="G3AEX3">
        <property type="interactions" value="1025"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEX3"/>
    <dbReference type="GeneID" id="18872720"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59015"/>
    <dbReference type="eggNOG" id="KOG3336">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067902_1_1_1"/>
    <dbReference type="InParanoid" id="G3AEX3"/>
    <dbReference type="OMA" id="YCPWNEK"/>
    <dbReference type="OrthoDB" id="407630at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006797">
        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037365">
        <property type="entry name" value="Slowmo/Ups"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11158">
        <property type="entry name" value="MSF1/PX19 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="201" mass="22904" checksum="EB52E2A2A8F71F07" modified="2011-11-16" version="1">MKFFESDHYFDYPWDQVTAANWQKYPNEMSTHVISVDILSRELNPATNVLRSERLIGCKQSIPKWLSFLVGGATVSYVREVSEVDLNNKTLVMKSSNLTMNNLLLVNETVVYRPDPELPQSRTLFEQSAEITAFASIGRICDKIEDWSVERFGQNAKVGKMAFEGVLKTLNEKWEQSGVFENTNNVLTEVSKLPFLQGSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEX7</accession>
    <name>G3AEX7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LicD/FKTN/FKRP nucleotidyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_64885</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373219.1">
        <property type="nucleotide sequence ID" value="XM_007373157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEX7"/>
    <dbReference type="GeneID" id="18875003"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64885"/>
    <dbReference type="eggNOG" id="ENOG502QREF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008074_2_0_1"/>
    <dbReference type="InParanoid" id="G3AEX7"/>
    <dbReference type="OrthoDB" id="444255at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009644">
        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF28">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE TRANSFERASE FKTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="5">
        <location>
            <begin position="402"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="748" mass="88101" checksum="B77BA04A480796A1" modified="2011-11-16" version="1">MLNSSYERKLLRIIPIILSINTIGTWLYYRFNQSSSSTIFFKDISSLPQVSFTNSNINRKLKSLHSKFKSPTFTSEITIPTTYFETSYLIHDPRVTISLTLNLLYHQIENNPENVSFPFNWADWVDLTYLNHQISKPENKRIKCSDLVEHMAFKNTRDKLKAKEDSMLFGCKNSYDLTKREIQEMGFTDLDKMPGFFQFQHSPRHTNEFIRVLQGKTYLLGRMPLPFQVIFLNDHGKDLKFTVDSKMNGRQIMEKYISNNNLKHKKKLTLDPAKEFDKLRVLLKSDTLKKVYKTRKIVRMNRSWFHYEPDDVNKEFKKLESKQQLTPVERGYLNSIIASRKTSNSKNRREPMYFKGANLIRGKANKDDGNHYEWRFFNGKWRDRYRHSLLLERILRNWFKFCQKYGIISWINYGSLLGWYRNGAIYPFDLDLDMQMSMYHMTILGKKFNQTLVVEDLHEGTGKYLIDVGTFIHNRYKIGVFLNHIDARFIDVDSGLYIDLTALASTKKYSDVHPKFFKDICDDPVEGPVLEDDGEIEVYNDRNDWVHKYNNISPLRLSMLEGVPVYIPKQIVKRMKFQYPCGTLNNFEFRDWYYVEQAGTWIHEKELLAVLDKSKAIQNGSVDKSNIKNQIKNLTDEQLYKLLSNDEATLSNYELARRNFGFHAKEIQYLFNIDAKLNRNKNDPPEGKVLDASPEGNHEYLRLIVDNVLLKAPHRDSICEYDRVKGKFADFHTIASRELDKIDVSDQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEY7</accession>
    <name>G3AEY7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_57865</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372203.1">
        <property type="nucleotide sequence ID" value="XM_007372141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEY7"/>
    <dbReference type="FunCoup" id="G3AEY7">
        <property type="interactions" value="820"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEY7"/>
    <dbReference type="GeneID" id="18872225"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57865"/>
    <dbReference type="eggNOG" id="KOG1753">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046483_4_0_1"/>
    <dbReference type="InParanoid" id="G3AEY7"/>
    <dbReference type="OMA" id="WPIEMAR"/>
    <dbReference type="OrthoDB" id="426865at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000007">
        <property type="entry name" value="40S ribosomal protein S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_uS9_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001749">
        <property type="entry name" value="PRK00474.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF16">
        <property type="entry name" value="RIBOSOMAL PROTEIN S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="142" mass="15723" checksum="222D2BA791FF434F" modified="2011-11-16" version="1">MSTQSVQTFGKKKTATAVAHVKAGKGLIKINGSPITLVQPEILRFKVYEPLTLVGLDKFQGIDIRVKVTGGGHVSQVYAIRQAIAKGLVAYHQKFVDEASKNELKKIFASYDKTLLVADSRRMEPKKFGGRGARARFQKSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFJ9</accession>
    <name>G3AFJ9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AN1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_48045</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372401.1">
        <property type="nucleotide sequence ID" value="XM_007372339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFJ9"/>
    <dbReference type="FunCoup" id="G3AFJ9">
        <property type="interactions" value="215"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFJ9"/>
    <dbReference type="GeneID" id="18871171"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48045"/>
    <dbReference type="eggNOG" id="KOG3183">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052358_2_1_1"/>
    <dbReference type="InParanoid" id="G3AFJ9"/>
    <dbReference type="OMA" id="RQYCLKH"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057358">
        <property type="entry name" value="UBL_ZFAND1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14677">
        <property type="entry name" value="ARSENITE INDUCUBLE RNA ASSOCIATED PROTEIN AIP-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14677:SF40">
        <property type="entry name" value="CDC48-ASSOCIATED UBIQUITIN-LIKE_ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25327">
        <property type="entry name" value="UBL_ZFAND1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="AN1-type" evidence="6">
        <location>
            <begin position="21"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="273" mass="30624" checksum="09A5F3C69BBC728B" modified="2011-11-16" version="1">MSTTNLFIDKTQHPTKDQGIIDIGKNCSYCNQLDFLPFVCEFCKQTFCSNHRKIDQHDCVNRDMFDRSPTRSVSPPSGVSSKTLFPDREADKKRINERLHSQSPRPTTILEKSFRVGDAAKGNSSAFKKFQKFLTIQNTTKAGGVFKIFKSSSASKSNYAEIATLKKVAKGDAKISAADRIYVWVLYISNPEEKLDIQQNRKAVFINKNWVVGRSLDSIADALHITNKNNVVTAAEDKLNIFKLENDEPKVVATSAKSSFRNGDTLYLVRGSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFP6</accession>
    <name>G3AFP6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_58169</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372447.1">
        <property type="nucleotide sequence ID" value="XM_007372385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFP6"/>
    <dbReference type="FunCoup" id="G3AFP6">
        <property type="interactions" value="1006"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFP6"/>
    <dbReference type="GeneID" id="18872354"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58169"/>
    <dbReference type="eggNOG" id="KOG3259">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090028_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFP6"/>
    <dbReference type="OMA" id="DEVQCLH"/>
    <dbReference type="OrthoDB" id="2530521at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000059">
        <property type="term" value="P:negative regulation of ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000749">
        <property type="term" value="P:positive regulation of rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045899">
        <property type="term" value="P:positive regulation of RNA polymerase II transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000010">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase Pin1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051370">
        <property type="entry name" value="PPIase_Pin1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000297">
        <property type="entry name" value="PPIase_PpiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10657">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10657:SF4">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00639">
        <property type="entry name" value="Rotamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50198">
        <property type="entry name" value="PPIC_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 5" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="WW" evidence="6">
        <location>
            <begin position="6"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="PpiC" evidence="7">
        <location>
            <begin position="65"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="177" mass="19696" checksum="A4BF761FABD3742A" modified="2011-11-16" version="1">MTSTNTGLPPSWTVRVSRTHNQEYFFNQATKESSWDAPFGTDQEVLSEYLKKFKANGYKPVVGDDGKVRISHLLIKNNQSRKPKSWKSPDGITTTRDEAIQILKKHLARILNGEVKLSELAKTESDCSSHSVGGDLGFFGKGQMQPKFEETAYALHVGEVSDIIETDSGVHILQRTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFT4</accession>
    <name>G3AFT4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_130696</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372485.1">
        <property type="nucleotide sequence ID" value="XM_007372423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFT4"/>
    <dbReference type="STRING" id="619300.G3AFT4"/>
    <dbReference type="GeneID" id="18869517"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_130696"/>
    <dbReference type="eggNOG" id="KOG2202">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059852_3_1_1"/>
    <dbReference type="InParanoid" id="G3AFT4"/>
    <dbReference type="OMA" id="MIDTRQA"/>
    <dbReference type="OrthoDB" id="423462at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089701">
        <property type="term" value="C:U2AF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12287">
        <property type="entry name" value="RRM_U2AF35_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000122">
        <property type="entry name" value="Splicing factor U2AF small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009145">
        <property type="entry name" value="U2AF_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12620">
        <property type="entry name" value="U2 SNRNP AUXILIARY FACTOR, SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01848">
        <property type="entry name" value="U2AUXFACTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="13"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="45"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="12">
        <location>
            <begin position="13"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="12">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW35073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="251" mass="29002" checksum="B1D65C37773AF1B8" modified="2011-11-16" version="1">MNRQYNNNNYQSRDNLVTCAFYNKIGACRHGEKCSKKHIKPTTSRTLLLSNLYQNPKLKTATTDDAEDELTPKQIQEIFDQFYRDIFVHFATTGEISQLVVCENENNHLNGNVYVRYKSETDASESMKQLNSEWFNGRPVHCELSPVDSFSEANCRAYETDVCSRGEHCNYMHVRKPTKKLADDLFKAQEKTRLLKRMQELIPKIKPEKEEHKNEVASLLSNPIAQHAVAEYQEKEPLESTTTSVVSQLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFU2</accession>
    <name>G3AFU2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
            <ecNumber evidence="8">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ARO4</name>
        <name evidence="10" type="ORF">SPAPADRAFT_58217</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the class-I DAHP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="8"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372492.1">
        <property type="nucleotide sequence ID" value="XM_007372430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFU2"/>
    <dbReference type="FunCoup" id="G3AFU2">
        <property type="interactions" value="224"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFU2"/>
    <dbReference type="GeneID" id="18872368"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58217"/>
    <dbReference type="eggNOG" id="ENOG502QPSU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030903_0_1_1"/>
    <dbReference type="InParanoid" id="G3AFU2"/>
    <dbReference type="OMA" id="PCLSWED"/>
    <dbReference type="OrthoDB" id="4699125at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000005">
        <property type="entry name" value="Phospho-2-dehydro-3-deoxyheptonate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006219">
        <property type="entry name" value="DAHP_synth_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00034">
        <property type="entry name" value="aroFGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009395">
        <property type="entry name" value="PRK12755.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE DAHP SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225:SF12">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE, TYROSINE-INHIBITED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001361">
        <property type="entry name" value="DAHP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP synthetase I/KDSA" evidence="9">
        <location>
            <begin position="49"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR001361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="366" mass="39646" checksum="7FA9FDD3BFEFCC33" modified="2011-11-16" version="1">MSNTPIPDEYDDTRILGYNPLIPPALLQHEIKASGKSLEVVIRGRYESAQILSGADDRCLVIVGPCSIHDTDAAMDYAKRLKALSEELKGDLLIIMRAYLEKPRTTVGWKGLINDPNVDNSFDINAGLRISRQLYADLTGKAELPIGSEMLDTISPQYFSDLLSFGAIGARTTESQLHRELASGLSFPIGFKNGTDGGLTVALDAVQASSKGHHFMGVTKNGMAAITTTKGNDNCFIILRGGKKITNYDAESVKAAKEAIAKSTDPNIKLMIDCSHDNSQKDYRNQPKVLDSVVEQITNGENTIIGVMIESHINEGKQGMPAPGCGKESLKYGVSITDGCVGWETTVDMLTKLSKAVQARRALNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGD3</accession>
    <name>G3AGD3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_53575</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372684.1">
        <property type="nucleotide sequence ID" value="XM_007372622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGD3"/>
    <dbReference type="FunCoup" id="G3AGD3">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGD3"/>
    <dbReference type="GeneID" id="18871796"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53575"/>
    <dbReference type="eggNOG" id="ENOG502QTTD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035743_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGD3"/>
    <dbReference type="OMA" id="HNLACLV"/>
    <dbReference type="OrthoDB" id="3996692at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="257"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="425"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="504"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="213"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="88"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="257"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="437"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="504"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="570" mass="64035" checksum="018F0D26BD5236A9" modified="2011-11-16" version="1">MIDSETLSKNSPFKITHSDSQNSYNLSNTTPDLLKFQLQEQYRNQLQDLKNSELPDLKLLNTNIFENSFNDHLQNQLELGNVNIVTRSDSSSPESTSTSLFSEPESTDGSTDIETVIASTKPNYLNLKVLIENSIFDTRNINKDSILSLSELQDLKTRIQIKNESKSYLLSKITVTQQFINDIIFKQTETIENNDNINTELGTKSLIKLLKSSSSLQSQLISINTELEELNTKLNNHNLSCLVLGYVEDIRLSSAAPSQTHSVSDPQSSAPLTPLDSPESRILSTPRGGDIYSKIYDTLFAHVASIAASRNITLPPPPSANSESFESRTRWVQQCIDTVLSNSKVDIAEIPPRDASMFSNASSILSPTSPIRNISQEKMLIEYKTALNDLRFSYQYLAKEYEHSRESFNKIILDYKKKITHLENESSKNKDDGLISPPSTRMSTCDSLDAKDKEISRLRKELNLLRVDNIRKDHSHLQGSTSSADLPDGASPELFSMMHLTVSNVSNDDGDETNSIHSNRPSSQGLSTGILRKEFKKIVSDIQDQYELELTQERLKSRQMAEELEQLKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGF9</accession>
    <name>G3AGF9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nuclear protein localization protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_58519</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the import of nuclear-targeted proteins into the nucleus and the export of poly(A) RNA out of the nucleus. Has a role in the endoplasmic reticulum-associated degradation (ERAD) pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NPL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372710.1">
        <property type="nucleotide sequence ID" value="XM_007372648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGF9"/>
    <dbReference type="FunCoup" id="G3AGF9">
        <property type="interactions" value="961"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGF9"/>
    <dbReference type="GeneID" id="18872483"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58519"/>
    <dbReference type="eggNOG" id="KOG2834">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017172_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGF9"/>
    <dbReference type="OMA" id="TKDRYVP"/>
    <dbReference type="OrthoDB" id="10251089at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036266">
        <property type="term" value="C:Cdc48p-Npl4p-Vms1p AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000837">
        <property type="term" value="C:Doa10p ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000839">
        <property type="term" value="C:Hrd1p ubiquitin ligase ERAD-L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030894">
        <property type="term" value="C:replisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036435">
        <property type="term" value="F:K48-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071629">
        <property type="term" value="P:cytoplasm protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006274">
        <property type="term" value="P:DNA replication termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099638">
        <property type="term" value="P:endosome to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072671">
        <property type="term" value="P:mitochondria-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900182">
        <property type="term" value="P:positive regulation of protein localization to nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072665">
        <property type="term" value="P:protein localization to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08061">
        <property type="entry name" value="MPN_NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016563">
        <property type="entry name" value="Npl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007717">
        <property type="entry name" value="NPL4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024682">
        <property type="entry name" value="Npl4_Ub-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007716">
        <property type="entry name" value="NPL4_Zn-bd_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12710">
        <property type="entry name" value="NUCLEAR PROTEIN LOCALIZATION 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12710:SF0">
        <property type="entry name" value="NUCLEAR PROTEIN LOCALIZATION PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05021">
        <property type="entry name" value="NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11543">
        <property type="entry name" value="UN_NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05020">
        <property type="entry name" value="zf-NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010052">
        <property type="entry name" value="Polyub_prc_Npl4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MPN" evidence="11">
        <location>
            <begin position="231"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW35298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="563" mass="63154" checksum="5D3B3FE715A74846" modified="2011-11-16" version="1">MASIILRFRSKDGMFRINTSSDSDFVSVLEQLLTKLSSAVNSQSLLLSNSPSQKGEPAKGYCGKTVQELGLKNGDMLFVSYETAIDPNAVPTSTPTVPITTTNLGPTKVQELPIDIALDKQEGLIARPLSSMCRHGARGMCEYCAPLPPWDENYRSEHAIKHISFHAYLKQQAEKHKNNGGSYIAPLEEPDYKIDLHCQQGHQPYPKGICSKCQPSPITLQQQKFRMVDHVEFADSSILNEFLNGWRSTGVQRFGYLYGRYEAFDKVPLGIKAVVESIYEPPQVGEFDGVTLLNWDDEQQIDAIASKLGLTKVGIAYTDLTDSGRKDGTVLCKRHKDSYFLTNLECIMAARFQHKYPNVTKYTSSGKFSSKFVTCVVSGGLLGEIEPRSYQVSTGAEALVEADIITGCTQPSMVYVNENSSTRYVPDIQYSKINEYGLEVKSNAKPTFPVDFLLVSLTDSFPVDPTPMFTSEYHYTIENREFMGDVQDLSTVYKYLNGGDYEVRKAKLCNFHFLCHLARLNILGPQEMESIFKFIVDKNEDDYIRLVESPGWMSLITILEENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGI0</accession>
    <name>G3AGI0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RFX-type winged-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_58545</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372731.1">
        <property type="nucleotide sequence ID" value="XM_007372669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGI0"/>
    <dbReference type="STRING" id="619300.G3AGI0"/>
    <dbReference type="GeneID" id="18872499"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58545"/>
    <dbReference type="eggNOG" id="ENOG502QVTM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_716805_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGI0"/>
    <dbReference type="OrthoDB" id="338531at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052406">
        <property type="entry name" value="Chromatin_Remodeling_Comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003150">
        <property type="entry name" value="DNA-bd_RFX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22970">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22970:SF14">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51526">
        <property type="entry name" value="RFX_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RFX-type winged-helix" evidence="5">
        <location>
            <begin position="149"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="386" mass="43662" checksum="73EC3E475BA2C2F7" modified="2011-11-16" version="1" fragment="single">MEEEDDVDERITNNCIDSITSSQLELIVNTLLVADNEMNNAVLEFLKMYLNSEAIHHDFISSPIKDSQKYRLQKLLQLSSTKANFNTLVKQLPRLIISHLPLNDPSSIKPIPPLNLTKRSDFAGVPSTLPDLPEELYSLIVRFSEPLRATTWLRCCYETFTPLSKPDESAENSSDVIPGEVTQISLWKAYEKQFQEVWDPPSGQTNPEFKQLLPAVEFIKNVSKAFPSSEAMVINLPPSASDSAPKKKFIIRGIQPRQFAVNIDVANYEALKPTVKTSSINPGENHKLPIGHVDVEKFNHLVSESSENILVEGTRISKNSEFVNQINESSHEILEYIINEVLDNTENSVEENIFRLYNSHWLPELVYANPSLVESGIINIGWLKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGK5</accession>
    <name>G3AGK5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_58572</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peroxiredoxin family. Prx5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372756.1">
        <property type="nucleotide sequence ID" value="XM_007372694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGK5"/>
    <dbReference type="STRING" id="619300.G3AGK5"/>
    <dbReference type="GeneID" id="18872508"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58572"/>
    <dbReference type="eggNOG" id="KOG0541">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072440_3_0_1"/>
    <dbReference type="InParanoid" id="G3AGK5"/>
    <dbReference type="OMA" id="SAWGKQH"/>
    <dbReference type="OrthoDB" id="1882547at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03013">
        <property type="entry name" value="PRX5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000159">
        <property type="entry name" value="Peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037944">
        <property type="entry name" value="PRX5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430">
        <property type="entry name" value="PEROXIREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430:SF39">
        <property type="entry name" value="PEROXISOMAL MEMBRANE ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 7" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5 7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="26"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="6">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR637944-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="186" mass="20351" checksum="37C2A91D600E240E" modified="2011-11-16" version="1">MFSLSRSLPKSRLFVSPLRNALRTYVAVGETLPSVDLYESSPGNAVNLAEETKSGKTIVIGAPGAFSPACSGTHIPGFVKNLRAFNDKGYQRFFVVSVNDPFVTKNWGEYLLHHTVAGQQIRFLADTNGEFTRELGLLFDATKVFGNERSKRYALLLEDGTITKAFIEPDNTSVDVSEASKVLAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH93</accession>
    <name>G3AH93_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_48510</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372935.1">
        <property type="nucleotide sequence ID" value="XM_007372873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH93"/>
    <dbReference type="GeneID" id="18871233"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48510"/>
    <dbReference type="HOGENOM" id="CLU_573864_0_0_1"/>
    <dbReference type="InParanoid" id="G3AH93"/>
    <dbReference type="OrthoDB" id="10504454at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="93"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="476" mass="54665" checksum="339C89D7CDCBEF66" modified="2011-11-16" version="1">MSRLLHILRGRSSGNSLSEKPNPQDQLTSMVGALNERPSQLSTIKDQLSAPAGSFNLESEKADSESKSDVATLIDLENKEVQTTEKKKVISDPTTLLSLPEELLLAIFVHLNQIDACQIRLVNKKLYKLGTIKLFNSIFVYNPEELAIASYNQVSKARYNYLAFSTRYTIINGFNDFVRVRGTKELSLVRNVVICIRSPNDHSNDCYNYLVEKCPWIKVDVASWSSDLFDKQFTRLDNITQLRVCKTFRFKTDITDNYSIKQLFISGSKRGVSDPVALIPHLKALTWLYINDAKEQDILQVFRSKKISKLKLSMLGLHVPQVPLKKLGDVFTLNEIFSLELHFYNNVTPYDDLKWLASQMTKLRDIHINWKDLSFERIIASFAGHTLHQIRLYSRGNEQEITEEQIARLLTGRERTVTYLSLDSGLSSLSGMCSYYRLYKSHTKAKHSVQNLRKKLPRKQYPNLMDLGIYHHVYAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHQ7</accession>
    <name>G3AHQ7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial import protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_135423</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373805.1">
        <property type="nucleotide sequence ID" value="XM_007373743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHQ7"/>
    <dbReference type="STRING" id="619300.G3AHQ7"/>
    <dbReference type="GeneID" id="18869889"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135423"/>
    <dbReference type="eggNOG" id="ENOG502SBHA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1556230_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHQ7"/>
    <dbReference type="OrthoDB" id="5529571at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070096">
        <property type="term" value="P:mitochondrial outer membrane translocase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013262">
        <property type="entry name" value="OMP_MIM1/TOM13_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28241">
        <property type="entry name" value="MITOCHONDRIAL IMPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28241:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08219">
        <property type="entry name" value="TOM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="19550" checksum="9CB21012472F1528" modified="2011-11-16" version="1">MSEYTQKPITPPQSESTPIEIDTTSDEVKEIYTLADTIRDDVFAVDALTEANDLTVLRAQLSSDQLVNDSDVLHTEELIEQSRIEEEEEEESEGSVYEVNVWSILQKGAINLVLPFINGMMLGFGEILAHEVGFRYGWLGAKVEPEGRVRKREQERQQQQQQQSQSSTSRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI09</accession>
    <name>G3AI09_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_134132</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373908.1">
        <property type="nucleotide sequence ID" value="XM_007373846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI09"/>
    <dbReference type="FunCoup" id="G3AI09">
        <property type="interactions" value="760"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI09"/>
    <dbReference type="GeneID" id="18869783"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134132"/>
    <dbReference type="eggNOG" id="KOG0318">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015246_1_0_1"/>
    <dbReference type="InParanoid" id="G3AI09"/>
    <dbReference type="OMA" id="FYQGPPF"/>
    <dbReference type="OrthoDB" id="2306at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003786">
        <property type="term" value="F:actin lateral binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032466">
        <property type="term" value="P:negative regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000102">
        <property type="entry name" value="Actin-interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19856:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19856">
        <property type="entry name" value="WD-REPEATCONTAINING PROTEIN WDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50960">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="56"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="197"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="240"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="326"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="531"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="616" mass="67375" checksum="490DE3EBEEA18C28" modified="2011-11-16" version="1">MSIKPIAITVPQPSTSRGQASHISYDPVNERLAYVNGKSIIVRPVDFKATAPVICFSKHICNTTAVSFSPSGFYVASGDESGQIKIWDASIDAKDKSTKFEQPTIKSEFQIMSGPIRSIAWDADNSRIIAVGQGKEKFGHAFTWDSGNSIGDIQGHSAAINAVAIKPQRPYRAATVGDDFALVFFNGPPFKFDKSLRGHHTNVVRDVKFSPDGEHLVSVSSDRSIVIYQGKTGEFVKRIENAHNGGIFAVAWLPDSKFFITASADNSLKKWDISTYESVQTFTVSEKVTVENHQVGLALAKEYVISLSANGNLNYFDYSGKLVEAVKGHQAPITTVKYDDDSLYTGGADGKLFQWSVQEDGPSALPESATGHTNYVVDIVKVGSSQVSVGWDDTLKLWNKENVSKSIQLPAQAKQLAKTSNGVIVLFENKIQIYTEDLSLVSELDFTFSSSSIGVVSDSELLITNTTTNTIEELRIESKSITKTSKEFPKMRSPPTLIVVSPSKEYFAVADAAGKYTLFKTSDASVVTTRWAFHSSRVYDAKWTPDSKYLISGGLDSGLFLYSVERPSKVVKYPLAHLSGISGLDWLSYDSEAKDGRFVTTGLDGTIRTWEVNISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AID3</accession>
    <name>G3AID3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Histone deacetylase</fullName>
            <ecNumber evidence="2 10">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_59836</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the histone deacetylase family. HD Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="2 10"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373987.1">
        <property type="nucleotide sequence ID" value="XM_007373925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AID3"/>
    <dbReference type="STRING" id="619300.G3AID3"/>
    <dbReference type="GeneID" id="18873096"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59836"/>
    <dbReference type="eggNOG" id="KOG1342">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007727_7_4_1"/>
    <dbReference type="InParanoid" id="G3AID3"/>
    <dbReference type="OMA" id="EFCKISC"/>
    <dbReference type="OrthoDB" id="1918432at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032221">
        <property type="term" value="C:Rpd3S complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000001">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF10">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037913">
        <property type="entry name" value="His_deacetylse_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="14">
        <location>
            <begin position="36"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW34403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="465" mass="52297" checksum="E32D65D97EBCD063" modified="2011-11-16" version="1">MYVELPFEELKPDTRKRRVAYFYDQDVGNYFYGTGHCMKPHRIRMTHSLIMNYELYKKMEIYRAKPATNLELTQFHTDEYIDFIDRVTPDNLQLFEREQIIFNVGDDCPVFDGLGEFCKISCGGSMEGAARLNNGSADIAINYAGGLHHAKKSEASGFCYLNDIVLGIIELLRVHPRVLYIDTDVHHGDGVEEAFYTNDRVMTCSFHKFGEFFPGTGNLNDIGIGKGKYYAVNVPLRDGIDDASYKSIFEPIIAAIVDWYQPSAIVLQCGGDSLSGDRLGPFNLSMRGHANCVNFVRSLGIPMMVVGGGGYTIRNVARTWAFETGICNGAILPKELPYNGYYEYYAPLYELDVRSSNMNNANSREYLDKILTQVISNLDHTKHAPSVQMNEVPVGMDLDEDLEEDLPESLDTKGGSQRARDKLVEAEGEFYDADEKEFGATNISNGYSLSESEKAEIEELNKQIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AID5</accession>
    <name>G3AID5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_49449</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373990.1">
        <property type="nucleotide sequence ID" value="XM_007373928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AID5"/>
    <dbReference type="GeneID" id="18871339"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49449"/>
    <dbReference type="HOGENOM" id="CLU_2279243_0_0_1"/>
    <dbReference type="InParanoid" id="G3AID5"/>
    <dbReference type="OrthoDB" id="3784at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="102" mass="9910" checksum="38021D4F6C9A3CE3" modified="2011-11-16" version="1">MPILAAIAAAHHGDHGSASSSMPSDHHDMASMSSGHHTGSTTVTSKPSAGTAAATSSTNGSQSTKKTTQASGSTTKTTQSGNGAGQASAGSAALFLLVWSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AID7</accession>
    <name>G3AID7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">mRNA-capping enzyme subunit beta</fullName>
            <ecNumber evidence="8">3.6.1.74</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">mRNA 5'-phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">mRNA 5'-triphosphate monophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_135454</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end diphospho-ribonucleoside in mRNA + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67004"/>
            <dbReference type="Rhea" id="RHEA-COMP:17164"/>
            <dbReference type="Rhea" id="RHEA-COMP:17165"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:167616"/>
            <dbReference type="ChEBI" id="CHEBI:167618"/>
            <dbReference type="EC" id="3.6.1.74"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:67005"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer. The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an mRNA 5'-triphosphate monophosphatase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the fungal TPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.74" evidence="8"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373991.1">
        <property type="nucleotide sequence ID" value="XM_007373929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AID7"/>
    <dbReference type="FunCoup" id="G3AID7">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AID7"/>
    <dbReference type="GeneID" id="18869892"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135454"/>
    <dbReference type="eggNOG" id="ENOG502RZAX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028201_0_0_1"/>
    <dbReference type="InParanoid" id="G3AID7"/>
    <dbReference type="OMA" id="DWVYATI"/>
    <dbReference type="OrthoDB" id="272147at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031533">
        <property type="term" value="C:mRNA capping enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140818">
        <property type="term" value="F:mRNA 5'-triphosphate monophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004651">
        <property type="term" value="F:polynucleotide 5'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07470">
        <property type="entry name" value="CYTH-like_mRNA_RTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.10">
        <property type="entry name" value="mRNA triphosphatase Cet1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040343">
        <property type="entry name" value="Cet1/Ctl1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004206">
        <property type="entry name" value="mRNA_triPase_Cet1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037009">
        <property type="entry name" value="mRNA_triPase_Cet1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28118:SF1">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-TRIPHOSPHATASE CTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28118">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-TRIPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02940">
        <property type="entry name" value="mRNA_triPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="CYTH-like phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="4 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="mRNA triphosphatase Cet1-like" evidence="10">
        <location>
            <begin position="226"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="36"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="505" mass="57691" checksum="B7190F4243893314" modified="2011-11-16" version="1">MNVGSILNNDSPPSDHEREASPTAPLPMRQPLPPQSTSARSSVSSTGRPSMASVHQRHSITNMLNDTPAEPPVKQEESFEIKKPDIGLFTTPTLSHKPPPVTSPFIKRNSIADITNENDIDITTGEILESKPKGIPSESEEEEDDLTKIKKKLSSMKPKRYETPPIWAQQWIPPNQQGAEIQLETNEPTNGNVQHTKLSDKHVFDYTTTRSVDLQCSITGVIPPASVTRTIAEWIYANFSNIEDRNRKYVELELKFGKIIDKRTGNRLNVNVVTECIFTDHSNIRYDMQVEEIAWQEVRKFFEELERNYQEEHKGHKQSGPRRKFNMLESDQTDSFYQIGGKGEHIKKVRVSKDNEINPPRYVAIQKERISDLYIHNPSSMYDLRLSLSLEIPVPENSIEPIITKNKPSLVREKKRTSWTHAPTITQFDLTRVLVPLESKNKQGKKIITHDITYEIELEIDTLEVFNAIDKITQGTDNFRLEELVEVFVNNARVINNRVTKLAVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIG3</accession>
    <name>G3AIG3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">C-4 methyl sterol oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SUR2.2</name>
        <name evidence="6" type="ORF">SPAPADRAFT_134612</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374017.1">
        <property type="nucleotide sequence ID" value="XM_007373955.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIG3"/>
    <dbReference type="FunCoup" id="G3AIG3">
        <property type="interactions" value="289"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIG3"/>
    <dbReference type="GeneID" id="18869838"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134612"/>
    <dbReference type="eggNOG" id="KOG0873">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047036_5_0_1"/>
    <dbReference type="InParanoid" id="G3AIG3"/>
    <dbReference type="OMA" id="WCAFTGN"/>
    <dbReference type="OrthoDB" id="1658724at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="5">
        <location>
            <begin position="142"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="298" mass="35315" checksum="E9E05D79F32D0371" modified="2011-11-16" version="1">MSSVYHDYSTFSNVTSFADSFANFRQLDNLNWFEQIWGAYYYYIGNDLFATGLLFFSVHELFYFGRCLPWAIIDRIPYFNKYKIQDEKIPSNKEQWECLKSVLTSHFLVEAFPIWFFHPLCQTIGISYQVPFPSITEMLIQWAVFFVLEDAWHYWFHRGLHYGVFYKYIHKQHHRYAAPFGLAAEYAHPVEVALLGLGTVGIPIVWCMITGKLHLFTVCIWIVMRLFQAVDAHSGYEFPWSLHHFLPFWAGADHHDEHHHYFIGGYASSFRWWDYVLGTEAGPKGKKQREEKMNKKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIK7</accession>
    <name>G3AIK7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">proteasome endopeptidase complex</fullName>
            <ecNumber evidence="3">3.4.25.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_134388</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="3"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373306.1">
        <property type="nucleotide sequence ID" value="XM_007373244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIK7"/>
    <dbReference type="FunCoup" id="G3AIK7">
        <property type="interactions" value="857"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIK7"/>
    <dbReference type="MEROPS" id="T01.011"/>
    <dbReference type="GeneID" id="18869809"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134388"/>
    <dbReference type="eggNOG" id="KOG0173">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_3_0_1"/>
    <dbReference type="InParanoid" id="G3AIK7"/>
    <dbReference type="OMA" id="KQHLFRH"/>
    <dbReference type="OrthoDB" id="429533at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03763">
        <property type="entry name" value="proteasome_beta_type_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000005">
        <property type="entry name" value="Proteasome subunit beta type-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024689">
        <property type="entry name" value="Proteasome_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF4">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12465">
        <property type="entry name" value="Pr_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="8 13" id="KW-0647">Proteasome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0888">Threonine protease</keyword>
    <feature type="domain" description="Proteasome beta subunit C-terminal" evidence="12">
        <location>
            <begin position="222"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW33722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="268" mass="28834" checksum="981B3B6C369CC9A8" modified="2011-11-16" version="1">MAGLNFENYQRNSYLSTKGFATPRATSTGTTIVGCKFPGGVVIAADTRATSGPIVADKNCEKLHRLAPKIWCAGAGTAADTEMVTQLIASNLELHALSQNREPRVITALTMLKQHLFKYQGHLGAYLICAGVDPTGAHLLSVQAHGSTDIGKYQSLGSGSLAAMAVLETNFREDLTKEEAIKLCADAIEAGIWNDLGSGSNVDVCVMEVGKDAELYRNYLTPNVRSQKARSYKFAPGTTAILRESVRDILDVEETVVSFDQMEVDTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIP2</accession>
    <name>G3AIP2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">leucine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Leucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_59883</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="2"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374042.1">
        <property type="nucleotide sequence ID" value="XM_007373980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIP2"/>
    <dbReference type="FunCoup" id="G3AIP2">
        <property type="interactions" value="1212"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIP2"/>
    <dbReference type="GeneID" id="18873115"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59883"/>
    <dbReference type="eggNOG" id="KOG0437">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004174_1_0_1"/>
    <dbReference type="InParanoid" id="G3AIP2"/>
    <dbReference type="OMA" id="IAWCEEM"/>
    <dbReference type="OrthoDB" id="10249672at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903432">
        <property type="term" value="P:regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07959">
        <property type="entry name" value="Anticodon_Ia_Leu_AEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004493">
        <property type="entry name" value="Leu-tRNA-synth_Ia_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055416">
        <property type="entry name" value="RBD_LARS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00395">
        <property type="entry name" value="leuS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794:SF1">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24810">
        <property type="entry name" value="RBD_LARS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="10">
        <location>
            <begin position="143"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="11">
        <location>
            <begin position="410"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine--tRNA ligase RagD-binding" evidence="12">
        <location>
            <begin position="561"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF24810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="697" mass="78766" checksum="BCE9D23409CD8D0D" modified="2011-11-16" version="1">METVLATKGTGVVTCVPSDSPDDFVTTRDLANKPEYYKIEKDWVKTDIVPIVQTEKYGDKCAEFLVNELKIQSPKDAVQLAQAKELAYKEGFYNGTMIIGKYAGEKVEAAKPKVKADLIASGEAFVYNEPEGPVMSRSGDDCVVSLEDQWYIDYGEDVWMGQALECLKDMETFCKETRHGFEGVLAWMKNWAVVRKFGLGTKLPWDHEFLVESLSDSTVYMAYYTISRFLHSDYYGKVQGKFDIKPEQLTDEVFDYIFTRRDDVDTDIPKDQLKAMRREFEYFYPLDVRISGKDLIPNHLTFFIYTHVALFPKKFWPRGVRANGHLMLNNAKMSKSTGNFMTLEQIVEKFGADASRIAMADAGDTVEDANFDESNANAAILRLTTLKEWCEEAVANKDKLRTGAQDNFFDQAFEHEMNQLVEATYKQYSLTNYKAALKTGLFDFQTARDYYRDSVSSTIGMHHDLVLKYIETQALMLAPIAPHFAEYIYKEVLGNSGSVQTASFPRASKPVDKAVIDALQYVKDVSRSIRESEANVLKKKKGGKPSDVDVTKPGKLTLFISNTFPEWQDKYIDLVKELFEQQTLDDKKVIATKVGKDMKRAMPFITLIKQRLTKEDPATVFNRKLTFNEVDVLKAVINNIKKAPYSIQVAEVDLIAFDNGAKTGVNILSGEEVEITITGKIVDSAVPGEPGIMITNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIQ4</accession>
    <name>G3AIQ4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidyl-tRNA hydrolase</fullName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_59893</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PTH2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374054.1">
        <property type="nucleotide sequence ID" value="XM_007373992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIQ4"/>
    <dbReference type="FunCoup" id="G3AIQ4">
        <property type="interactions" value="984"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIQ4"/>
    <dbReference type="GeneID" id="18873124"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59893"/>
    <dbReference type="eggNOG" id="KOG3282">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073661_0_1_1"/>
    <dbReference type="InParanoid" id="G3AIQ4"/>
    <dbReference type="OMA" id="RMDLGMT"/>
    <dbReference type="OrthoDB" id="1733656at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032435">
        <property type="term" value="P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02430">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1490.10:FF:000001">
        <property type="entry name" value="Peptidyl-tRNA hydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bit1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00283">
        <property type="entry name" value="arch_pth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649:SF11">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="Peptidyl-tRNA hydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="34"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="57"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="202" mass="21826" checksum="D7A1B9E1D0D2E390" modified="2011-11-16" version="1">MTSNSNLQILSVGIVSFLTGYYVSQYLKCSNRRKLKQKKPPIAPGSSSSDASTESSYESDSDESDDGIDVDSTDLNTVPGEVRMTLIVRQDLKMGKGKAAAQCSHATLALYKKITNPNSPAYNPEMVRRWEYGNGQAKITLQVPDQEGMDMLYAQAISLGINAYIVHDAGRTQIAAGSATVLGLGPAPKSIIDQITSELKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIS5</accession>
    <name>G3AIS5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatidylglycerol/phosphatidylinositol transfer protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_59923</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the intermembrane transfer of phosphatidylglycerol and phosphatidylinositol.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NPC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374075.1">
        <property type="nucleotide sequence ID" value="XM_007374013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIS5"/>
    <dbReference type="FunCoup" id="G3AIS5">
        <property type="interactions" value="108"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIS5"/>
    <dbReference type="GeneID" id="18873136"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59923"/>
    <dbReference type="eggNOG" id="KOG4680">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097982_0_1_1"/>
    <dbReference type="InParanoid" id="G3AIS5"/>
    <dbReference type="OMA" id="HQTYDLC"/>
    <dbReference type="OrthoDB" id="6409159at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00917">
        <property type="entry name" value="PG-PI_TP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.770:FF:000004">
        <property type="entry name" value="Phosphatidylglycerol/phosphatidylinositol transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003172">
        <property type="entry name" value="ML_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033917">
        <property type="entry name" value="ML_PG-PI_TP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039670">
        <property type="entry name" value="NPC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306">
        <property type="entry name" value="NIEMANN PICK TYPE C2 PROTEIN NPC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306:SF0">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL_PHOSPHATIDYLINOSITOL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02221">
        <property type="entry name" value="E1_DerP2_DerF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00737">
        <property type="entry name" value="ML"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442135" description="Phosphatidylglycerol/phosphatidylinositol transfer protein" evidence="8">
        <location>
            <begin position="19"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="MD-2-related lipid-recognition" evidence="9">
        <location>
            <begin position="62"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="190" mass="20853" checksum="14F2643593F8EAAF" modified="2011-11-16" version="1">MVSFKHLALLTLVGSANCLSVHATTGYFNQAAQLFKSNPDQSVLNLPPPHHDTEPIPGDSPLEKCDITEVQLLTLEEINLLPNPPKAGANLTFTATGFVGKTIEPGAYVDVDVRYGFIKLIHQTFDLCDEVEKVDLQCPINKGNQVISKIVAIPEEVPPGRYFVTARAYTKDDEYITCLSAVIDFPVVRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIS8</accession>
    <name>G3AIS8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_59932</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374078.1">
        <property type="nucleotide sequence ID" value="XM_007374016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIS8"/>
    <dbReference type="STRING" id="619300.G3AIS8"/>
    <dbReference type="GeneID" id="18873137"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59932"/>
    <dbReference type="eggNOG" id="ENOG502SBS6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142967_1_0_1"/>
    <dbReference type="InParanoid" id="G3AIS8"/>
    <dbReference type="OMA" id="GYQKNDP"/>
    <dbReference type="OrthoDB" id="10252718at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="98" mass="11731" checksum="45CEC9CAE433286D" modified="2011-11-16" version="1">MARLDNYDNHKRPELVSFDDIDYDNFPDVVKARKSMMRENMIRTEELKIAHEALRKCADYHKQDGDRNCRALALKYMKMLDDFRPQGYLGYQRNDPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIW2</accession>
    <name>G3AIW2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_48890</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373357.1">
        <property type="nucleotide sequence ID" value="XM_007373295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIW2"/>
    <dbReference type="GeneID" id="18871282"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48890"/>
    <dbReference type="eggNOG" id="ENOG502RQ81">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112097_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIW2"/>
    <dbReference type="OMA" id="WINFIFT"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024316">
        <property type="entry name" value="APQ12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12716">
        <property type="entry name" value="Apq12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="198" mass="22416" checksum="E6CF40C3E654D2E5" modified="2011-11-16" version="1">MSVNDILISVGAVVLTFLQIILSNAIAALVFAHRRYPDLTAITVVLLGSYATYRILKKSAKMWINFIFTLIKLVVVLTIVLSGVMIYFRGWKFYEQDISFIYHAFQGILNLSGFMNETQIENFVSQIGIAFSKLNSQESAEPNEFAEFVNYIKSNFDFKDMGANYDYINELAGQGAEYLDKVDLKDFAEGLFGHVNQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIZ1</accession>
    <name>G3AIZ1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Striatin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_59159</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373386.1">
        <property type="nucleotide sequence ID" value="XM_007373324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIZ1"/>
    <dbReference type="STRING" id="619300.G3AIZ1"/>
    <dbReference type="GeneID" id="18872799"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59159"/>
    <dbReference type="eggNOG" id="KOG0642">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_497120_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIZ1"/>
    <dbReference type="OMA" id="MKYKIVQ"/>
    <dbReference type="OrthoDB" id="727118at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013258">
        <property type="entry name" value="Striatin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051488">
        <property type="entry name" value="WD_repeat_striatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15653:SF0">
        <property type="entry name" value="CONNECTOR OF KINASE TO AP-1, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15653">
        <property type="entry name" value="STRIATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08232">
        <property type="entry name" value="Striatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Striatin N-terminal" evidence="4">
        <location>
            <begin position="49"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="272"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="272"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="290"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW33802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="548" mass="61260" checksum="53DAFFD9E9D3AB48" modified="2011-11-16" version="1">MSTSKSQANSKSGNQSINPQAQWHQQGPSSQQQQLSQSQQSQSTTEPYTLPGVINYLTSEFTNLERYKIMTNLEKSEMKYKIVQLQGEVNSLKFANKKQKARIEALEEENKRLKGIAGSDTTDKSEVKDDFIIPEVDLSIIKNSRQQLTKSMREIVNLLKTPSSKNINYLNLPDPNDSLNNEYDVLVNHSAQEEADTDNVESLLFKELSAGKSHIAPNSIISQFFSDNNNKEEESMSDVKIPSPKFDDDVIYNDGLERVITQDSEAATMIMDEEEDDDDDDDNGAAVADDREEEFDEKEDDIPGVISPFPSPEPEINDFNDPTYEILDVYDHNNITIYLNSMTDNKQIKLTAVRKSDNKVKLSKSFNLTSSASKISNIFPISVDEEIFLLVDKQGEIKSLEMGEIPQETVLAKPAFTSVQSNDLIEFTSKSTVNGKSFGLAITGASSNGFLSKIHQINYKSGDKLTSKEIGSYNKRFLTKGKSGDVTFIGWYVNDKATVTLEQSKSKSKNAVSCDDIELAPYELLYKVDDKTVELNIVSKQSSYLSAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ23</accession>
    <name>G3AJ23_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glucosidase 2 subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_133564</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374119.1">
        <property type="nucleotide sequence ID" value="XM_007374057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ23"/>
    <dbReference type="STRING" id="619300.G3AJ23"/>
    <dbReference type="GeneID" id="18869759"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133564"/>
    <dbReference type="eggNOG" id="KOG2397">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016834_2_1_1"/>
    <dbReference type="InParanoid" id="G3AJ23"/>
    <dbReference type="OMA" id="KHESASH"/>
    <dbReference type="OrthoDB" id="28322at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017177">
        <property type="term" value="C:glucosidase II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039794">
        <property type="entry name" value="Gtb1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036607">
        <property type="entry name" value="PRKCSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028146">
        <property type="entry name" value="PRKCSH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12630:SF1">
        <property type="entry name" value="GLUCOSIDASE 2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12630">
        <property type="entry name" value="N-LINKED OLIGOSACCHARIDE PROCESSING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12999">
        <property type="entry name" value="PRKCSH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13015">
        <property type="entry name" value="PRKCSH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442359" description="Glucosidase 2 subunit beta" evidence="6">
        <location>
            <begin position="17"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="7">
        <location>
            <begin position="353"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="149"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="264"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="327"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="474" mass="54270" checksum="E2AB03D3F5146ABA" modified="2011-11-16" version="1">MLKLIAITYLAASVACSIKGVSPENQHLYEPVIIDDVQYWHCLNDSSIRLTYNQINDNYCDCPDGSDEPGTNACENSKYYCENKGHFPAYIDSFKLNDGVCDYDVCCDGSDEPEGCENKCDVIHNQYVEYKENVEKFMATSLQTKEKYLQVAKTKKEKLSNDKTRLEQQLSSAQKQLERLRLELENKELEAELEEPSVYEALSDHFVNLNQKLQANKEILFTKNSHIDTLEQILHTLANNYNPNFNDPAVKESINKFKQYISNKQEVNNDIGDAQNVLEKLQQQAKSLTQGGGVVPTIANTFHYYYQLFTNTFLVAPQVPNLSNLGNNELSSQIDQLETQVKNIQRKLQEVVSNLNINAGPNDILRAFDTINKKISGYHYRISLLDSIYQDDVLIGRFKEFKDNKVYFDYGSRCWNGPQRAGDVEFVCGKGPDIVSVSEPEKCHYNFVIQGESWCEPMTEEEIRANFNIDYDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ67</accession>
    <name>G3AJ67_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_59184</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373408.1">
        <property type="nucleotide sequence ID" value="XM_007373346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ67"/>
    <dbReference type="FunCoup" id="G3AJ67">
        <property type="interactions" value="285"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ67"/>
    <dbReference type="GeneID" id="18872811"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59184"/>
    <dbReference type="eggNOG" id="KOG0108">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_758611_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ67"/>
    <dbReference type="OMA" id="EMGFINY"/>
    <dbReference type="OrthoDB" id="272703at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.70">
        <property type="entry name" value="Transcription termination and cleavage factor, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025742">
        <property type="entry name" value="CSTF2_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026896">
        <property type="entry name" value="CSTF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038192">
        <property type="entry name" value="CSTF_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735:SF2">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14327">
        <property type="entry name" value="CSTF2_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14304">
        <property type="entry name" value="CSTF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="7"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="236"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW33824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="347" mass="37478" checksum="EE1DB46F55546792" modified="2011-11-16" version="1">MAPVNSNILYLGSIPFDWDEETVKSVVCGSGNIVDVRLGFDYAGKNKGYCFIEYQTVSDAQRALPLLSQVMLMGGKRLRVEASKEGLKGSKQGAAEKKPVLMLSRTKLPDYVKLPNELLLAGGNGGRINSPVPSTIPNIPLNRMSTGQMPVKFTNATKNFPQPVALPFGIPDKINETLSKIPPVQLIELIATMKNSSGAGPIHEILQVSPDLAACAAQALLLMGFIDGDVISDSMKSASATPQPLQPPPQHTPLQTTPQPYINQPTFHAPPPPVSNPYGTPYQGNIPAAPPVNQPWLSQETQAKLAGVPPDQKELIMQILSLPPDQVRSLPIDKQEMIKNLQARYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ95</accession>
    <name>G3AJ95_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59219</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373436.1">
        <property type="nucleotide sequence ID" value="XM_007373374.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ95"/>
    <dbReference type="FunCoup" id="G3AJ95">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ95"/>
    <dbReference type="GeneID" id="18872823"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59219"/>
    <dbReference type="eggNOG" id="KOG0698">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021251_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ95"/>
    <dbReference type="OMA" id="LPNWGNW"/>
    <dbReference type="OrthoDB" id="416093at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004741">
        <property type="term" value="F:[pyruvate dehydrogenase (acetyl-transferring)]-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901524">
        <property type="term" value="P:regulation of mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF589">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE [ACETYL-TRANSFERRING]]-PHOSPHATASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="54"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="436" mass="49430" checksum="4CC004D081087B43" modified="2011-11-16" version="1">MCRTNVTQRCMKVQFRTVSTAITFTAVDRSPSIPMSTTKPLTLGKLRVPLLKSPSHLGHVTARVNRLYNEDRYSAKVLNFDNKTLFNFSVFDGHGGEGCSDYLAENLSKEIEEAQQVMNSEAEEELVKRYRDDVSGYWKRWYKHRQENFDLYENFKIELKNFPELASDNLSLRLLLSFLEADYKFAQAATDNSGSTCTTALIETIYSEPGVFQPYFENYYFNRHTISQLTIGQVGDTRAILVDKNGLANCLTEEHHPSNPTEATRLRKYSTNLFMTDSFGEERFVHLANTRAFGDFRYKQIGVSAEPDIVQYIIGDKAKIKQFLTPEEIKKYTINGLGGDESFLVLLSDGITNILTDQEVADIVMTHFDLKGHTVATPQSCAEEVVKFVEYVGGDDNATCLVVRLNGWGKWPSIDRTGELRQSRLVDFNPRSRGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJC7</accession>
    <name>G3AJC7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60016</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374170.1">
        <property type="nucleotide sequence ID" value="XM_007374108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJC7"/>
    <dbReference type="FunCoup" id="G3AJC7">
        <property type="interactions" value="1420"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJC7"/>
    <dbReference type="GeneID" id="18873181"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60016"/>
    <dbReference type="eggNOG" id="ENOG502S7SS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049337_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJC7"/>
    <dbReference type="OMA" id="QFAYERA"/>
    <dbReference type="OrthoDB" id="5778525at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="379" mass="43734" checksum="D6B0C0951447EF12" modified="2011-11-16" version="1">MNIHNLDTGQHQPHDKSHSQSQQNGSDPSMSFLDLLSSDMDFEQAYSMYNNLQTKMAHGTVEELQKVQMLNQQYQTNLTQPTEPVRNEDEFAYGRAQILNGNGIISNYMNKNYSFDMAKDNKPEGEDDFDQFFSNTESNALEKFLDNLTNPNASGDPLQFYINKSQSEGHKRFKQEEDEFNPSFEMHTMKLPQLNKTYHEPNKNIIYPAAQFPPTQHSLPTPNTTRQSSSNSANSSYILDDFKRRANDINGMIITPPTSGDETKTQEDKPQVKRKKKRVTHKPLLSLEQKRLNHSHSEQKRRQLCKLAYQRCLKQIIDLEAFNKLPAISEEQRKSKKARISKDGLPNLSKHNALIRISNEMILIKRLNEELKKLIDKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJJ9</accession>
    <name>G3AJJ9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Malate synthase</fullName>
            <ecNumber evidence="3 10">2.3.3.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_59277</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>glyoxylate + acetyl-CoA + H2O = (S)-malate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the malate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.9" evidence="3 10"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373486.1">
        <property type="nucleotide sequence ID" value="XM_007373424.1"/>
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        <property type="interactions" value="533"/>
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    <dbReference type="STRING" id="619300.G3AJJ9"/>
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    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59277"/>
    <dbReference type="eggNOG" id="KOG1261">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018928_3_0_1"/>
    <dbReference type="InParanoid" id="G3AJJ9"/>
    <dbReference type="OMA" id="DGSWIAH"/>
    <dbReference type="OrthoDB" id="186072at2759"/>
    <dbReference type="UniPathway" id="UPA00703">
        <property type="reaction ID" value="UER00720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004474">
        <property type="term" value="F:malate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Malate synthase"/>
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    </dbReference>
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        <property type="entry name" value="Malate synthase, domain 3"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.20.1220.12">
        <property type="entry name" value="Malate synthase, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044856">
        <property type="entry name" value="Malate_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011076">
        <property type="entry name" value="Malate_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006252">
        <property type="entry name" value="Malate_synthA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019830">
        <property type="entry name" value="Malate_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001465">
        <property type="entry name" value="Malate_synthase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048355">
        <property type="entry name" value="MS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048356">
        <property type="entry name" value="MS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046363">
        <property type="entry name" value="MS_N_TIM-barrel_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01344">
        <property type="entry name" value="malate_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42902">
        <property type="entry name" value="MALATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42902:SF1">
        <property type="entry name" value="MALATE SYNTHASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20659">
        <property type="entry name" value="MS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20656">
        <property type="entry name" value="MS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01274">
        <property type="entry name" value="MS_TIM-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001363">
        <property type="entry name" value="Malate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51645">
        <property type="entry name" value="Malate synthase G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00510">
        <property type="entry name" value="MALATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Malate synthase N-terminal" evidence="12">
        <location>
            <begin position="27"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Malate synthase TIM barrel" evidence="11">
        <location>
            <begin position="179"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="Malate synthase C-terminal" evidence="13">
        <location>
            <begin position="428"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001363-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20656"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF20659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW33902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="559" mass="63578" checksum="B978D2402BC8C0DD" modified="2011-11-16" version="1">MTTSNSLYTSLHGVQVLAQVEKQAQYESKTSQADILTQSALSFVVLLHRTFNATRLQLLQNRMNLQRDLDNSAKLDFLKDEFTTKIRNDPTWTGPTLAPGLIDRRTEITGPPERNMIVNALNTPVKTYMCDFEDSSTPTWSNILNGHVNLYDCIRHQIDFVNKTNGKTYAVNWDTNTPTLMVRPRGWHMDEKHILVDGQPLSGSLMDFGLYFYHNAKQLLENGTGPYFYLPKMEHHLEAKLWNDVFNVAQDLLGITRGTIRATVLIETLPAAYQMEEIIYQLRQHSAGLNCGRWDYIFSTIKRLRSSATLPNRDLITMKVPFMEAYCKRLINICHRRQVHAMGGMAAIIPIKNDVEANEIAMDKVAQDKLREAKMHYDGTWIAHPALAPIANNIFNDYMPTANQIHIVPDENVTAEDLSNTKIEGGVITTEGIRLNVYIALCYIESWIRGVGCVPINNLMEDAATAEVSRLQLYTWCKNKVFLDDTKQALTPKYVGEILDEEIAKLQKVYNSPKFQFEKAGNYIRQELGGELVSEFLTTLLYDEVTTIGSPIDLQYLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJK3</accession>
    <name>G3AJK3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_65111</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373490.1">
        <property type="nucleotide sequence ID" value="XM_007373428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJK3"/>
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        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="GeneID" id="18875018"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65111"/>
    <dbReference type="eggNOG" id="ENOG502RA62">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_731907_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJK3"/>
    <dbReference type="OrthoDB" id="4020846at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00105">
        <property type="entry name" value="KH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="2">
        <location>
            <begin position="290"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="378" mass="42836" checksum="4B115E38FDB4BAE9" modified="2011-11-16" version="1">MPMVLGLDPIYQDSQVQQQGITTGTMDVQLELNEDSLSSLLEGEIYSTSILQPDLDYNQILDEFNLETEIIDLNEGEYSNYSLTDIENQENSIDSFNLDDLSLDSTGVGYTFSEIPQLVYSNNKITFPCNIESIRDSIDIQLVLSSSSLIYLLQTLANMGIADYSTTIVAKRNLLSLDWLVDIPKESYYAAIVLGIINDHQASLTFVGDVLTCQKLIRHCQNVATIVFDNKKIQVMDIHNLAKFSDDICDFTDDRIKPLPINHFTAHREHPIDKIAIRKHSEQVQPKEEKMKTVKLILAQREVSSLIGIKGHRLNSIRIQSRCLIKVLPINQETPALLSRREKPQELLIQGTESNVAIAVHEINTFVSQQRLNNNKFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJU2</accession>
    <name>G3AJU2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pheromone alpha factor receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_133541</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373577.1">
        <property type="nucleotide sequence ID" value="XM_007373515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJU2"/>
    <dbReference type="FunCoup" id="G3AJU2">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJU2"/>
    <dbReference type="GeneID" id="18869757"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133541"/>
    <dbReference type="eggNOG" id="ENOG502QTV4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038593_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJU2"/>
    <dbReference type="OMA" id="MWMISKK"/>
    <dbReference type="OrthoDB" id="5402633at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038038">
        <property type="term" value="C:G protein-coupled receptor homodimeric complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004932">
        <property type="term" value="F:mating-type factor pheromone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14939">
        <property type="entry name" value="7tmD_STE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.920">
        <property type="entry name" value="Pheromone alpha factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000366">
        <property type="entry name" value="GPCR_STE2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027458">
        <property type="entry name" value="STE2_TM1-TM2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28009">
        <property type="entry name" value="PHEROMONE ALPHA FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28009:SF1">
        <property type="entry name" value="PHEROMONE ALPHA FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02116">
        <property type="entry name" value="STE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00250">
        <property type="entry name" value="GPCRSTE2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="390" mass="43488" checksum="C9A096E4AB6AC10D" modified="2011-11-16" version="1">MDENFETLGNSLLADIDNSGDILLTYAFPNIDDIQVFPFKVLDDYQRRVLESSIVFGVSIGACSVILLLLFVVSYNNRKKKLFKSFLFNLNAAILLVYIIQSGLNLNYLMSRLSSPSFFITGAYYGESFAMTDTANAFRVILVGLVQISLTYQVYIIFKGHRLNIWSVSATLFSTLFSITVVAFTINSVVLAHRKNSAFTHGQERNFVSLWNDLPVWLFSININIMSVLLILKLGMAIRTRRYLGLKQFDGLHILVIMSTQTLIIPSIILFVHYFGISNVGSMLVKVSMLLIVLTLPLTSLWAQTQNNTENINSSPSMSFIRREISTVTTFSNNRGGNTSNLEKKPSSTVQGIIFPDLSTNPGSALPSAIEQLLGEGDDTISNPISWEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKC4</accession>
    <name>G3AKC4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Golgi to ER traffic protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Guided entry of tail-anchored proteins 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GET1</name>
        <name evidence="11" type="ORF">SPAPADRAFT_136875</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET2, acts as a membrane receptor for soluble GET3, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Golgi to ER traffic (GET) complex, which is composed of GET1, GET2 and GET3. Within the complex, GET1 and GET2 form a heterotetramer which is stabilized by phosphatidylinositol binding and which binds to the GET3 homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Golgi apparatus membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the WRB/GET1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375099.1">
        <property type="nucleotide sequence ID" value="XM_007375037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKC4"/>
    <dbReference type="FunCoup" id="G3AKC4">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKC4"/>
    <dbReference type="GeneID" id="18870003"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136875"/>
    <dbReference type="eggNOG" id="KOG4253">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089418_2_0_1"/>
    <dbReference type="InParanoid" id="G3AKC4"/>
    <dbReference type="OMA" id="AEWIISF"/>
    <dbReference type="OrthoDB" id="1741719at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03113">
        <property type="entry name" value="Get1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028945">
        <property type="entry name" value="Get1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027538">
        <property type="entry name" value="Get1_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42650:SF1">
        <property type="entry name" value="GUIDED ENTRY OF TAIL-ANCHORED PROTEINS FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42650">
        <property type="entry name" value="TAIL-ANCHORED PROTEIN INSERTION RECEPTOR WRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04420">
        <property type="entry name" value="CHD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 9" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="topological domain" description="Lumenal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="9">
        <location>
            <begin position="182"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="209" mass="23962" checksum="68498AC5939286EA" modified="2011-11-16" version="1">MLTLDIDPYTVVVSVFVILLTQQIIKQIGKSTIQEFLWLGYIHYGTKLNFSSQFTQLAQAKEQLHQLNKEKRTISAQDEYAKWTKLNRKCDKLTADIASLTEDISQSRASIDKLTGRLIMLSTTLPLWFFRIFSRKTHLFYFSSGIFPWYIERVLSLPFLPAGAVGLTIWMYAVNSVLSNVLFLISFAFAGKKVKPAKPEKKIVEEKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKK1</accession>
    <name>G3AKK1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60941</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375121.1">
        <property type="nucleotide sequence ID" value="XM_007375059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKK1"/>
    <dbReference type="STRING" id="619300.G3AKK1"/>
    <dbReference type="GeneID" id="18873648"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60941"/>
    <dbReference type="eggNOG" id="KOG0937">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_742209_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKK1"/>
    <dbReference type="OrthoDB" id="10259133at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="7">
        <location>
            <begin position="1"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="52"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="373" mass="43533" checksum="5F41A340D8531D60" modified="2011-11-16" version="1">MIEDCEFIYDLSTNIIKRNEIYGTCVVKSYLSGMPICRVGFNEKHMTLISESEVTSNEEIPKNQLQLEDDNDEEEEVALEDSEEPSDKSKSRRKIPIGNIQFHQCIELSKVYKDNLVTFIPPDDKFILMTYNVDQQKQKRKLPLIMVKPKFKVINQSHKLQIMCVINTNFNRRRHCKNLYVKIPINQNHFQIRYDGSDSLKYKAELGEVSYKVDSSEIVWRIDSIDGKKTARMMAEVSLLKEVSKEKIDNYVNRRIIPELSTEVADIQDNAKQELDEFYGVNKINNTTQRIISTASSARSLHDDIELTFQIPMMTYSGLKLTYLSVEEEQLKYPCFPWVRYITKSSHSDTSRGFTGRQCYYRFKLGVDNFIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKM3</accession>
    <name>G3AKM3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_60966</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375143.1">
        <property type="nucleotide sequence ID" value="XM_007375081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKM3"/>
    <dbReference type="GeneID" id="18873659"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60966"/>
    <dbReference type="HOGENOM" id="CLU_2887261_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKM3"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="63" mass="6944" checksum="66663D0C53F4043E" modified="2011-11-16" version="1">MMVPIPTAEEEETEAQVDQEIVTNSEITMKEVVNPAQITLTMVPIGDKLLVVLVYSLNKPSCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKP3</accession>
    <name>G3AKP3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF1682-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_60286</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007374400.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="G3AKP3">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKP3"/>
    <dbReference type="GeneID" id="18873318"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60286"/>
    <dbReference type="eggNOG" id="KOG2357">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042570_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKP3"/>
    <dbReference type="OMA" id="FDGFVWA"/>
    <dbReference type="OrthoDB" id="10039147at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032469">
        <property type="term" value="P:endoplasmic reticulum calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012879">
        <property type="entry name" value="CCDC47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12883">
        <property type="entry name" value="ADIPOCYTE-SPECIFIC PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12883:SF0">
        <property type="entry name" value="PAT COMPLEX SUBUNIT CCDC47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07946">
        <property type="entry name" value="CCDC47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="325"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="325"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="358"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW32947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="370" mass="42755" checksum="701033B8393AD533" modified="2011-11-16" version="1">MIFEVLNKLLPAGMQQGVSDYSEFTLEELTAMSIWQRIAIRNWSLEIVTVSFIFSFIAIYKLGDLYNQSKATTFLKGISPVLKKHFYQVGVTGDKLYVKDSAENFSSYATGRTNIAKVDIKISLQPRQNLFVWILEYVLGFFTGSVKIPTDKAEITITPSVQYDNFIVAIVSKLGMNDARKFNYFLSLCKTNDSTNLPESFVYMSEANEFQDKFSIPELNKALTLQSASFVKFIAATDQPVERPESLREMIPQRRLVVSVDISNKKDDLALVQQVLEALFVFVDRIADKSITFRPEALKKVVKTREVEIEKLKKIQEEIQQEKVAEEKAQQKRAERDRLRNLSREEQIKLEKKEMEKKQKKLQRKQKVRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKS7</accession>
    <name>G3AKS7_SPAPN</name>
    <protein>
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            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">SPAPADRAFT_60309</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374496.1">
        <property type="nucleotide sequence ID" value="XM_007374434.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKS7"/>
    <dbReference type="STRING" id="619300.G3AKS7"/>
    <dbReference type="GeneID" id="18873335"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60309"/>
    <dbReference type="eggNOG" id="ENOG502S7DH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1300356_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKS7"/>
    <dbReference type="OrthoDB" id="4087488at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="212" mass="25130" checksum="D596AF7171136504" modified="2011-11-16" version="1">MRLFKGYSYASSIKQEYFPFINLRTKYARFFTNFEVDDMNHNKWSIGSIPRVRISKMNKFKSKVKHSINEEVEQDEVDEYKLIGKRNIYFHRNYFTHNPFIYSHETKFPPVLSMFRQYETKSVKRIKQSVKKKRQSLINHDKYIAQEEDLGTKYYNGSDGLYFSGSNQDDNPDDNKLGWITIYDDETLSNKGMFEFVLGMTLAVGFDSCLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKZ3</accession>
    <name>G3AKZ3_SPAPN</name>
    <protein>
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            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">SPAPADRAFT_135605</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374551.1">
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    </dbReference>
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    <dbReference type="STRING" id="619300.G3AKZ3"/>
    <dbReference type="GeneID" id="18869905"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135605"/>
    <dbReference type="eggNOG" id="ENOG502T74Z">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091756_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKZ3"/>
    <dbReference type="OMA" id="MIRGNWS"/>
    <dbReference type="OrthoDB" id="4091959at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12622">
        <property type="entry name" value="NpwBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="229" mass="26822" checksum="2FC9F7908115815C" modified="2011-11-16" version="1">MIRGNWSLAGELRKNERSQQVKVQKRQKHQHKLTKLKDIDPIKLYHRIKNLEDKSEKTERDDNYLKSLKEDWAFIEKNKLHESKIRTFLDQQEKDEKKRKKESTKLWGSKSVYFNPELNPLGKVPQSESLVTKLKHPLTNITTPLKKDQTKKYEIDPLIKQLNIKCPPEDPPKFYKFVQNTTKPKVKPAEEQPHLVSENTELNHESSSENSANDDSSDDEGVSKRIKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL77</accession>
    <name>G3AL77_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_50037</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374635.1">
        <property type="nucleotide sequence ID" value="XM_007374573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL77"/>
    <dbReference type="STRING" id="619300.G3AL77"/>
    <dbReference type="GeneID" id="18871396"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50037"/>
    <dbReference type="eggNOG" id="KOG2294">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005476_0_0_1"/>
    <dbReference type="InParanoid" id="G3AL77"/>
    <dbReference type="OMA" id="SYYMHTY"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22701">
        <property type="entry name" value="FHA_FKH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045178">
        <property type="entry name" value="Fhl1/FHA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21712">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN FHL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21712:SF29">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN FHL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="6">
        <location>
            <begin position="190"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Fork-head" evidence="7">
        <location>
            <begin position="671"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="3">
        <location>
            <begin position="671"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="95"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="303"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="483"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="849"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="978"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1065"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="748"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="116"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="317"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="483"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="494"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="509"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="522"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="857"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1080"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1160"/>
            <end position="1186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1186" mass="130131" checksum="FEB5F3FD1576903B" modified="2011-11-16" version="1">MSSIGDDSISAMVKPHDNHQGKELVEHESKFSPSEDSSRVVGSVPNIGSLNLDEEINNLLDNHDDVSGSNTNIISTRSNTLHNLLPELDLANLDHESGNTVTGAHNNNHEDEEPRTVSNSQSPSLQPTDASKPVSLIQQAIRQKSNLVSVTSDGTDGSQNHDEGNHHSRVSAYARLDFENFTFFVQTLQVVLGRKSNDELLQTSHHSVDIHLSSKKAISRRHAKIFYNFGTQRFEISILGRNGAFVDEVFVEKGLTIPLKDGTKIQIGDIPFEFVLPSIEPSEEDETKLNPSDVINLRSAIFNKKSPSPQPLPHPPPSEKSKSSPKKDAKIKLEDVKNDDLFKQPTKPAERKNSISRRDSILKIRRLSTTRRKSQAANDEINELLKDLGVTSIDALAEENSDLLDAQIQSLLAEADADVDAEVDTDMREYDESPIIDDEEDEITKLENNEINDIEMNLTVLDQEIASLAPLIDAHNQELMKEKEEKKKVLEKEKKKKQQQQLQRRRSSMVKSTVTSGILANSDSSPSSTTTPRSAPLMGKPASIQPPASRLYNRTIINGITSTSTTDGKSPAFAATSPTYVPKQIIPPRPPPPKLEVPVLLITSEPAATRSRLPLRAITVSDSSYLATFDVPKTIEEPSKYPKQKLRKDIPRKPAKKVYSQDEIPEQHRSKPNVTFMMIITSVLKTEAARNGLTINEITEAIKEIYPYYKYCQDGWQFSISHCIKFNKIYKRLMKRGTEWLFTMDDLYMNEREKARKKQQEIAAARAKAEAARQEELRQKQKLEAQQAMSQNLAARNFNSPYGIPNARFSQNQFVSQSPYNKQLPISDGQKPKTIAELASEIRRDGVGSKAPMYFKPTTTSPPIATTPGANTIKAQLAANRASSPTPSMSDPNTQKSLEYLRKELFVLYRGRKLTYDKQTATVLITKALATTIAQVNSIGAKAGCGENALGFLVERAPEQVSKILDIALTKSIKEHEGTYTNTNSRATTPVPTPSPSKPETNTSIAATTTTQIPPAVSSPSKPVSPPVAVKLEQPQAKAITTPPIIGTDNPVKAVVDAAAAAARNETKPVSQQSPVISKASVASTPTTTPQPSSATSSPPVSTSPSLAKPPSFVSSKPKGDSLGRPSALGRPQSFGKPGSSGALSRPPTFLSNKPAFRSNETEQGVKRELEGSSVDEQAKKVQRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL83</accession>
    <name>G3AL83_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein FUN34</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60437</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetate uptake transporter (AceTr) (TC 2.A.96) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374641.1">
        <property type="nucleotide sequence ID" value="XM_007374579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL83"/>
    <dbReference type="FunCoup" id="G3AL83">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL83"/>
    <dbReference type="GeneID" id="18873408"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60437"/>
    <dbReference type="eggNOG" id="ENOG502QUJS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051062_0_0_1"/>
    <dbReference type="InParanoid" id="G3AL83"/>
    <dbReference type="OMA" id="WKKGNTF"/>
    <dbReference type="OrthoDB" id="3648309at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015123">
        <property type="term" value="F:acetate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051633">
        <property type="entry name" value="AceTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000791">
        <property type="entry name" value="Gpr1/Fun34/SatP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047622">
        <property type="entry name" value="GPR1_FUN34_YAAH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038013">
        <property type="entry name" value="AceTr_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123:SF1">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01184">
        <property type="entry name" value="Gpr1_Fun34_YaaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01114">
        <property type="entry name" value="GPR1_FUN34_YAAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="264" mass="28447" checksum="C55B7129EFF0E3E4" modified="2011-11-16" version="1">MSDNQQLEIESKEGPSGLTPDHPDHPVTKVQTDGDYVQFGNERYLRSELVEAFGGTLNPGLSPPPKHDFANPAPLGLSAFALTTFVLSLINCEARGVTIPNIVVGLAFFYGGAAQLVAGMFEIAVGNTFGGVALSSYGGFWGSWAAIQVDAFGIKAAYAEEPKQFANAIGIFLIGWFIFTFFMMLMTVKSTVAFFMVFFLLTITFLLLAISEFNGSTAVKKAGGVFGLLTAFTAWYNAYAGIANPQNSYITIKGVQLPDFQNRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALA1</accession>
    <name>G3ALA1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60450</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374659.1">
        <property type="nucleotide sequence ID" value="XM_007374597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALA1"/>
    <dbReference type="STRING" id="619300.G3ALA1"/>
    <dbReference type="GeneID" id="18873419"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60450"/>
    <dbReference type="eggNOG" id="KOG0022">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_2_1"/>
    <dbReference type="InParanoid" id="G3ALA1"/>
    <dbReference type="OMA" id="VHGVWSC"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08254">
        <property type="entry name" value="hydroxyacyl_CoA_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="7">
        <location>
            <begin position="29"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="6">
        <location>
            <begin position="189"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="352" mass="38617" checksum="54E669D2E93D7E1E" modified="2011-11-16" version="1">MKTQTAYGFQLGSHQLQKFTNHPIPTPKSNEILLKIEAAGLCLSDPHLLIAGKPMESNQPVADKFVMGHEIAGSIAEVGAGLVDHPVYKRGGRFAMQIGIACGACHACREGHDASCEESSQAYGLNEDGGFQQYLLVRNLRTLLPIPDNVSYEVAAVTTDSVLTPYHAIQKVKHLLQPTTKILVQGCGGLGLNGIQILKNYPGYIVATDTKPATEKIAREFGAHEFHLDISKSHHDPYSFDIVFDFVGAQVTFNNSDKYIKRRGKIVMVGLGQSKLFIPNYLLARREIEIIFNFGGTSAEQLECMEWVSRGLIKPKVTVVDFETLPQAILDLEHGKLTGRVAFKPNASGNKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALJ5</accession>
    <name>G3ALJ5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60572</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374753.1">
        <property type="nucleotide sequence ID" value="XM_007374691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALJ5"/>
    <dbReference type="GeneID" id="18873474"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60572"/>
    <dbReference type="HOGENOM" id="CLU_1850016_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALJ5"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="139" mass="15315" checksum="75632F74F3B25FC3" modified="2011-11-16" version="1" fragment="single">MADIGHPSQTIKPESHQTPTIVTAPALVTMIETLNPSVHTKFTSQQQHFSNNAVPPEHANSESAPDNGNHPEPQQQQQQQHLNHRLDKQREDNQIPVTSSSSLSLSNPSPTKPNASSQESIEHNKLESSSELSFTKKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMA3</accession>
    <name>G3AMA3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_60760</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374916.1">
        <property type="nucleotide sequence ID" value="XM_007374854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMA3"/>
    <dbReference type="FunCoup" id="G3AMA3">
        <property type="interactions" value="159"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMA3"/>
    <dbReference type="GeneID" id="18873551"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60760"/>
    <dbReference type="eggNOG" id="KOG2056">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040577_1_0_1"/>
    <dbReference type="InParanoid" id="G3AMA3"/>
    <dbReference type="OMA" id="WRQIYKG"/>
    <dbReference type="OrthoDB" id="4033880at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16992">
        <property type="entry name" value="ENTH_Ent3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000006">
        <property type="entry name" value="Clathrin interactor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF45">
        <property type="entry name" value="CLATHRIN INTERACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="2">
        <location>
            <begin position="25"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="230"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="303"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="400"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="427"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="457" mass="50030" checksum="CE8C0DAD58311D6C" modified="2011-11-16" version="1">MDLLKDAAKNLNLYEVKAYVRKAQNVAMNLTEMEAKVREATNNEPWGAPSTLMTQIAAGTYNYREREEIIGFIFRRFTEKAANEWRQIYKSLQLLDYLIKNGSERIIDDVRANLSLIQMLKSFHYIDSKGRDQGINVRNRAKTLIALLNDDSLIRSERKKARANSKKFGGVSSAAFGGASSITTGPTYDDDFTNRVYGDGGVYGERYDDPAAAYSNGQTGKDNFEEYDVGSSTPSSGSSKPTTSSSRVTAHAAAKASKSKPQPKKPEEDLLGDLLSFDGSGSSAPAQHAAPADEDDDDDFDDFQAAPSQPQQQQQPTLSNNLSNLYHQQPQQQPQQPIFAQQQQQQQQHQQPLFGQPPQQQRVASFGSGANYNVSTSTGPAKPAANVDAFSSLFSSAKTKTKTTSQPSSTIPSHTATPIAKNDDDFFGGFNSNNNNNTNQTNNTSAKNDGEIDLLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMH4</accession>
    <name>G3AMH4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein GCN4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GCN4</name>
        <name evidence="12" type="ORF">SPAPADRAFT_60169</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the bZIP family. GCN4 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374341.1">
        <property type="nucleotide sequence ID" value="XM_007374279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMH4"/>
    <dbReference type="FunCoup" id="G3AMH4">
        <property type="interactions" value="3544"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMH4"/>
    <dbReference type="GeneID" id="18873257"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60169"/>
    <dbReference type="eggNOG" id="KOG0837">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068501_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMH4"/>
    <dbReference type="OMA" id="IDHTPMF"/>
    <dbReference type="OrthoDB" id="5419235at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001080">
        <property type="term" value="P:nitrogen catabolite activation of transcription from RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903833">
        <property type="term" value="P:positive regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12193">
        <property type="entry name" value="bZIP_GCN4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12192">
        <property type="entry name" value="GCN4_cent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001491">
        <property type="entry name" value="Cross-pathway control protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050946">
        <property type="entry name" value="AP-1_TF_bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11462">
        <property type="entry name" value="JUN TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11462:SF35">
        <property type="entry name" value="TRANSCRIPTION FACTOR JRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="11">
        <location>
            <begin position="247"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="188"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="259"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="143"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="193"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="301" mass="32955" checksum="628BDE9DAFD99500" modified="2011-11-16" version="1">MSATTPMLFEDSLFESQILIASDEHTTNDVVVPATKQIHVEPELGSALPMERKPGPASPMPIHSSVLDSVFSCTMDELENTPMFDELDLIVDGSKVNTKDDWVSLFGAVTSTNDEPLLSLDDLGAIDDEEIVPRDEAFNSDATVSAESANTTATSSPGVAPSTTATSMTIGSKRRFTEVEESFEFSVPNQLFTPNPSSTLPTPLLDSKKKSTSSKVDHLGCVTYSKKSRSQPLQPVVADSSDPVSLKRAKNTEAARRSRARKMERMTQLETRVEELIDEKTGLEQEVLRLRELLTKNGIQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMI8</accession>
    <name>G3AMI8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoribosyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_136453</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374947.1">
        <property type="nucleotide sequence ID" value="XM_007374885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMI8"/>
    <dbReference type="FunCoup" id="G3AMI8">
        <property type="interactions" value="109"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMI8"/>
    <dbReference type="GeneID" id="18869974"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136453"/>
    <dbReference type="eggNOG" id="ENOG502QRN9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092544_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMI8"/>
    <dbReference type="OMA" id="IMKTGNY"/>
    <dbReference type="OrthoDB" id="9973266at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052657">
        <property type="term" value="F:guanine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000310">
        <property type="term" value="F:xanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032263">
        <property type="term" value="P:GMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046100">
        <property type="term" value="P:hypoxanthine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032265">
        <property type="term" value="P:XMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000033">
        <property type="entry name" value="Xanthine phosphoribosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363">
        <property type="entry name" value="HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363:SF1">
        <property type="entry name" value="HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="3">
        <location>
            <begin position="13"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="215" mass="24240" checksum="49264CCF7739D1F5" modified="2011-11-16" version="1">MSDEEVNKMYISYNNIHQLCQEQADKIKQFKPDLIIAIGGGGFIPARMLRSFLKEPGQPNVRIMAIILSLYEEVSAEDGGVDVIGHQVVRTQWIDYNQSKVDLVGKNVLIIDEVDDTRTTLHYAVSELKKDVAEQAKAKGADPNATKFGIFVLHDKQKPKKADLPDEIMKTGNYFAARTVPDAWIAYPWESTDIVYHQKRAEEQGNDVFLPSTTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMU3</accession>
    <name>G3AMU3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Alkaline phosphatase</fullName>
            <ecNumber evidence="3 15">3.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_55826</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 1 Mg(2+) ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.1" evidence="3 15"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375633.1">
        <property type="nucleotide sequence ID" value="XM_007375571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMU3"/>
    <dbReference type="FunCoup" id="G3AMU3">
        <property type="interactions" value="201"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMU3"/>
    <dbReference type="GeneID" id="18871975"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55826"/>
    <dbReference type="eggNOG" id="KOG4126">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008539_6_0_1"/>
    <dbReference type="InParanoid" id="G3AMU3"/>
    <dbReference type="OMA" id="KAAGYMT"/>
    <dbReference type="OrthoDB" id="7392499at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047386">
        <property type="term" value="F:fructose-2,6-bisphosphate 6-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009166">
        <property type="term" value="P:nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.60.40:FF:000002">
        <property type="entry name" value="Alkaline phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000063">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018299">
        <property type="entry name" value="Alkaline_phosphatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596:SF5">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00123">
        <property type="entry name" value="ALKALINE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 13 15" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="12">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="479"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW32357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="561" mass="61302" checksum="79AD6D4C0296580E" modified="2011-11-16" version="1">MSRSKENSPLLGGESLSCYNSKKDKKKVYLSYLLNVVTLSLLAYFFLIPSSHNHDNKNGGSSGAPPHVDQSKKKNIIMMVTDGMGPASMSAARSFRQFRDGLAINDVLTLDKYLIGSSRTRSSSSLVTDSAAGATAFSCALKSYNGAIGVRPDSSPCGTILEALKLQGYLTGLVVTTRITDATPAAFSSHVDFRSQEDMIAQHQLGEYPLGRMVDLILGGGRCHFYPSGVAGGCRADKRNLIEEAQRNGTWSYVGDRASFDQLDGGHNVSLPLLGLLADTDIPYDIDRDSTEHPSLAEQVKVALTALSKATENSEQGFFLLIEGSRIDHAGHHNDPAAQVREVLAYDAAFAEVIKFIDESDVDTVAISTSDHETGGLVTARQVSEEYPDYLWYPEVLNNSKHSGDFLAQKIYKAKISDEKKLHKFVTKEILEADMGITNYTEDEVKSVVENASNPAKLLYVLNNILSLRAQIGWTTHGHSAVDVNIYAYTNSERLNAKLHSKEPYVGLAGNHENIEIGAFMESISTADLHKVTELIQDTEHSPKKKDSLTADEFHANAVQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMV0</accession>
    <name>G3AMV0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61445</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375640.1">
        <property type="nucleotide sequence ID" value="XM_007375578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMV0"/>
    <dbReference type="STRING" id="619300.G3AMV0"/>
    <dbReference type="GeneID" id="18873896"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61445"/>
    <dbReference type="HOGENOM" id="CLU_2127076_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMV0"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="114" mass="12638" checksum="C4AC0ACBF80D1BA5" modified="2011-11-16" version="1" fragment="single">MFKNANKDAIMSPHRPVRISTPEKPRFSFDKSKTTKLRKTIQSPYTTHSPITSTPLLTAAAVGVETAEIPCQSSQNFENYDDDLDFDSPSKTRKSSGLSIISSNSATIYQEQPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMZ1</accession>
    <name>G3AMZ1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HTH CENPB-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_50964</name>
    </gene>
    <organism evidence="5">
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375681.1">
        <property type="nucleotide sequence ID" value="XM_007375619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMZ1"/>
    <dbReference type="FunCoup" id="G3AMZ1">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMZ1"/>
    <dbReference type="GeneID" id="18871503"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50964"/>
    <dbReference type="eggNOG" id="KOG3105">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019142_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMZ1"/>
    <dbReference type="OMA" id="EFKILYR"/>
    <dbReference type="OrthoDB" id="125347at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050863">
        <property type="entry name" value="CenT-Element_Derived"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004875">
        <property type="entry name" value="DDE_SF_endonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006600">
        <property type="entry name" value="HTH_CenpB_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303:SF73">
        <property type="entry name" value="PROTEIN PDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303">
        <property type="entry name" value="TRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03184">
        <property type="entry name" value="DDE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03221">
        <property type="entry name" value="HTH_Tnp_Tc5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00674">
        <property type="entry name" value="CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51253">
        <property type="entry name" value="HTH_CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH CENPB-type" evidence="3">
        <location>
            <begin position="44"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="624"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="691"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="626"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="656"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="691"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="778" mass="88686" checksum="B6065EEA51E389E6" modified="2011-11-16" version="1">MTQSDLATWAMKQYGATKPPSQTTISRILSSKNDLIASKEADFQLVRRRQQTNPLLRRILTEWITQAIWENIPVTTPIIQSTANAIWTRLPKEEKDGNGVFNQKWCNHFIKKLNINLTGSEKDKAENPGGYTLNKVWKLDEKIELKQYLDLLIAKEHYTPQDIFVLDEFQLFYSLPLDQIFDVSSIDKGLKQSSSSTENSLTIMLGCNIDGSEKLSPLIVGKYDKFDVSKSTHSGLKNISVDSISQPALMNKITEAYRIFYKSNTNKWITSSMFQNYLLTLDHKIGSVTPNRKILMLLDDSSSHRIINLKFEHIRLVYLKNDTNHKNPYSTTYSGSKFDYLPMNFGIIEEFKILYRLQQYLEMINLQRNNSKVDKMVNSSSQIDLNKYDISSNTPGLEVLSETDYHIPMIRVIEWITRSWHSITQERIFSSWKKTHLFKLAGKDWPSEKSRPAAEYSLLPLNTRMSNYDESKSYNKLEEIMRYLNVVIPWEIDELLGLVNERGKVTLSYASITEIIGSCLSESFDSKVKQNKEAKSNLISDQWYPGVGESDAAANNSLFFNSKATTFEFPYQEESRLIENNSIEQLLLKAPSLTPADLETNSVQHPTPLSSRFTSVSPTVSLTTTQIPQYPSNESSLRTKHKLPESWSEMNKKRPNNSLNYHYLPSQPNGSVQLPEAVLSDQSAQYNTMDATSGFSQQSRQQTPTSLGKADSQDILQSGKPDLINEFELITSLNRILEASSSNALKLSPTTIEELNFNLRMIQSKFGNSSENSYNSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMZ8</accession>
    <name>G3AMZ8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phospholipase D1</fullName>
            <ecNumber evidence="3">3.1.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Choline phosphatase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Phosphatidylcholine-hydrolyzing phospholipase D1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_67029</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phospholipase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="3"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375688.1">
        <property type="nucleotide sequence ID" value="XM_007375626.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AMZ8">
        <property type="interactions" value="140"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMZ8"/>
    <dbReference type="GeneID" id="18875132"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67029"/>
    <dbReference type="eggNOG" id="KOG1329">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000690_3_0_1"/>
    <dbReference type="InParanoid" id="G3AMZ8"/>
    <dbReference type="OMA" id="DSLWTKH"/>
    <dbReference type="OrthoDB" id="14911at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031321">
        <property type="term" value="P:ascospore-type prospore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000753">
        <property type="term" value="P:cell morphogenesis involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01254">
        <property type="entry name" value="PH_PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09138">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09141">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000011">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF76">
        <property type="entry name" value="PHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00614">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="12">
        <location>
            <begin position="760"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="12">
        <location>
            <begin position="1070"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="362"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="418"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1398"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1527"/>
            <end position="1683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="120"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="383"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1398"/>
            <end position="1408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1527"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1537"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1549"/>
            <end position="1558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1622"/>
            <end position="1640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1643"/>
            <end position="1658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1659"/>
            <end position="1673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1674"/>
            <end position="1683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW32412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1746" mass="200948" checksum="D8F54B6E287DCBBE" modified="2011-11-16" version="1">MLDQIDTNTDHQNGTTKENNNHLEPSSQEPNGSKTVNEEDGLLRKLSHSLSRQELFVPRHGKERGRSQSPLRETPDISKIFKTVSRETRSERKSESDPRRSRSLSDSPHIVSNPFSFFNKNDDKNELRYRSDEEEEEQHMRTSIPEILSSPSSMPPQALNHEDLDDGHFHRRESRIPFTHSQSFQNFFPSMFGRPGSQYETPVNDGHDAFHLTPDLTKQQKLKKRWQNFYRNRKRDRRRPSVNIGYIDEIREEAEKQTGVRAQKLIGGLALGGPAINLMASCLLEDERGISRAPILLSLIGLNISDVSLSDTTRIRKFKIDLEYGVGPQRMKWCIERNAKDLLYLHSKFKFESWIGNKNSQLPKFPKPPFKKSEMMKLKRTRTTQTQHATTTEDQGGDNDSESSFSLIPRLGFGSISSMTSNENKGQPPPDEAESRRRNAQYVKDVESYLKELIHLVSLKPQSNRLFVFFEISPISSLLSYETGYVGKQGVVHISGSARSQGWRVGHFKANDLKGMIDRRSEKWMLVRNSYVMYVSDINSTSPLEVFLVDSDFKITSKHEDHITEDVESDFDDSSLVQRRMALLEDSDASNIVFPHLKITLENSERKLILNPKSAKEQKLWISSLMEMKNSTIWSEKHRFGAFAPVRENCFAQWLVDGRDYFWAVSSALEMAKDVIFIHDWWLSPEIYLRRPANGNQQWRLDRILQRKAKQGVKIFVIIYRNVGSTVSIDSLYTKHSILSLNDENIHVIRSPNQLLQNTYFWAHHEKICIVDQTVAFVGGIDLCYGRYDTPDHALVDDSKIDFQSLGPEDIPTNDDVVNFQTFPGKDYSNPRVKDFFDLNKPYESMYDRNITPRMPWHDVHMFTAGKLARDLSRHFVQRWNYLLRQKRPSRFTPLLTPPPDLTDEEALMYGMDGTCEVQLLRSCGNWSLGLKEHEQSIQTAYLKLIESSEHFVYIENQFFVTSCLIEGTEIQNRIGDALVDRIIRAHEEGTVWKAVIVIPLMPGFESQVDQAEGSSVRVIMQCQYMSISRGETSIFAKLRKKGINPDDYIQFFSLRKWGRIGVNRTLVTEQLYIHAKIMLVDDRAAIIGSANINERSMRGVRDSEVAIIVRDKEMIASTMNGEPYLAGKFAHTLRMRLMREHLGVDVDILDVVERRFKKFENLARSMDDSTFETNKFKNIENRYLSAMVEIASRDILNEPEGTRRWRNYSIASESSLKTTKVEFEEEDEFDLPPPMFLPISLNNRTGPHEANKGIRDKKKHSYDNRVQNNAGHKQDVFGTGLDKYKSKLAKRARLNSGRFLKDLSVKFIKSNPVGSFLPDIDHVREFLEADDIHMTDEMDEESERIISERNKERWLLLKKISYLQRVASKQNRSNEVENKRRMSAGMAALYSNLPKTAESTEMSTGTPDPSDKASSDGNNINRPGISLYKKYPIVSLDENSIKDLVKSITSSEANVSKFVDPYGFEDPVDDEFYDFLWNEHARRNTDLFRMVFHSQPDDVVSRWSDYTHFTSLYSTFMKAQDLSADLRQEGTREGSQEAEEVVEIEEETEKKNIKPEDLGLLGKVPPEREEIPSPSSPESKMRFRLGKRTSISLGSPKLENSIPAEKVGEVEEEETKPPQIEESEESPNDSPNESDESPNEEPVSREISGEALKEKTASKSPPLQKPSASFSKAHSRTRRKRAGTYLARTKMNSGEIIYERDTAERLLSEIQGHLVYYPTEWLNVELDNNNWFYNTDRLPPMEIYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN86</accession>
    <name>G3AN86_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_55914</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375745.1">
        <property type="nucleotide sequence ID" value="XM_007375683.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AN86">
        <property type="interactions" value="767"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN86"/>
    <dbReference type="GeneID" id="18871986"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55914"/>
    <dbReference type="eggNOG" id="KOG1848">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001169_1_0_1"/>
    <dbReference type="InParanoid" id="G3AN86"/>
    <dbReference type="OMA" id="AWRLCLN"/>
    <dbReference type="OrthoDB" id="294853at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032817">
        <property type="entry name" value="Mon2_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16206">
        <property type="entry name" value="Mon2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Mon2/Sec7/BIG1-like dimerisation and cyclophilin-binding" evidence="5">
        <location>
            <begin position="4"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2/Sec7/BIG1-like HUS" evidence="3">
        <location>
            <begin position="202"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2 C-terminal" evidence="4">
        <location>
            <begin position="895"/>
            <end position="1033"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW32469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1577" mass="179762" checksum="B579AD050BA54B6B" modified="2011-11-16" version="1">MSSIHSLIGELTNLSTESKRRFTETRHACDKAVAALKSYPSWDIQIKDISQPQHKQEITRPVILACKSGNAKLTTISLSIIHKLIMAQLIPIDTLKELLNALLEASHLAIDIQLRILQCLPSFMQSYSEQFTGELLLILLEICSSLTANNKSTVVINTASATLQQLFSNIYDKIKELPEGVAGTHQIKIDNDEIVTVDDLSNEGYLIFQDLCHFIENEKPTYLKESIHIKLLSVLEIVENIIASHTTIFLKHQELAYLLRVKVVPSMLRILNSPLKPFPLVDRTMRIIYVLLSTQLENLEIESEVVLSFLNHLLLDGSGNTAAGEFVEPDWEKVLVLELFKGLFSDFQVMKSIYTKFDYNKDKKNVVLELLTILGTYLSNNSQLLQEEIRPIPKSWSVSSDQQHVYLSKSTSGLKTSILDHLDKSEPPTGIPPTYTIYLIHTILTSFCEGIANFVASLSEGPGAADLEEDVQLINALISRTAKEITGLYHRFIYTIMDEECFHLLIRSMQRFTHTTGLLGLVEIRDDLLLTLSRAIINNIPHKEVGRSPTPETGKQPFGFGFVETISSSLNDANGAVVKEETDISTSSVHSRYFNSRHITCIRALANLAISLGSTLKSSWTIIWVTFQWCDYFINGHDEFFGYIRGKVKMAGPDGKKPDLSNADIVNVQTSMKKLLESIGDYNVDSYYDVVDSLTSLSNREIGEGKQEEEDTDLHTCPYNKSYFLNKLVQVCEINGKKFLIEDNKSWELIQGYFIELGSCRTLHSNVRISITEAFTNVIKTVSHDGFEQQDDEETIHITSEKSLESLGKYLNSLVTLGLAQELLVLNCETDIYLTILTTLHELIDKYDTFYQKSWNKVFHILNLPFTISGNGATADKFRLLVEKSYDTLKLILDEFLSSLPFDQYKLLIDTLGNFVDQKYDLNISFSSVSYFWLISDSLKSRIVMFSTERTKQHIPESVVSDAELVEFITSSPESLDLYTCLDIYLLSSLTKISQVEERAQVKNGSIQTFFQIIDVHGSLLGNCWDLIYEIVLPKLFSMIPKPDEFGKVGDKTVLESYNLMLDGFTSLYNKFMRNKEIEGIVVKWQALIDYLKRLLSSNSVDLKLQVFKSFHDLLVSVYELSGSLTEIRNMVFRLWVEVPVEYDFINIKYQECLVELMKCFPDLYKIIISKLTLDEANTIINMFNKCARYPILPTNQLDDVKPTSLQASVIENLNLIDVKESPAIQSLVIQQLGVMIIYPFGIRSRIESKLTSNKLITDKFKLPTFVALGHVSLEILLEKLNSFEDSQFSVLIQDRGALKCLKSFIEIIDNHAEGVKSREKPAWILANQGIYFIIKQLLAHDSSVQDDEMWKLIIQDIKLCFQSTQENQDVKIEQYKELSELILPNLLARTKNDSTELLSEYVEILYDNSFLYEFNEWEKTVFDQYKNNNSSDELIEQLTNYPFEEVFGTTTPLVKYDNYKSRITCLQELIKFSKPSFSGTTRELQTISQKFLVARASFCSRRFISDQRLLYKCPIPIVQQRELMIILNGLGELRGEHDNKTNQTKLYRLLIKLIPYATRIDGAGYKLTKVLDTLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANF8</accession>
    <name>G3ANF8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61062</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375223.1">
        <property type="nucleotide sequence ID" value="XM_007375161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANF8"/>
    <dbReference type="GeneID" id="18873699"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61062"/>
    <dbReference type="eggNOG" id="ENOG502RS0T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_968257_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANF8"/>
    <dbReference type="OrthoDB" id="4094906at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="288" mass="32789" checksum="25481DA238A52832" modified="2011-11-16" version="1" fragment="single">VIQEKYYEIRYHENKHSNFIVPTIRTLQRQLAAALKKATTRRNHQSSGIQQHHTVPSTLEEIERDSYYDFAKVIPDSSLSELEDALYDLHEVSEKIKQIKFNLNTNSNSVNASPLHGGSSASMSSSSIQQIIDSSLADATSPIIHSNQHLPKSPLSSSITTPVSIRPKSGSNENVVIDHIQNLRNNLTDSSTSNNSDSFSKGLDMLELILKQTIELHQSIHNENETFRKEIDRLTKDHLRKVSEMNQNHLIRQRDLNRQFLELFKTEFESRENSDHILARLASLEALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANN5</accession>
    <name>G3ANN5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome c oxidase assembly protein COX19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_71943</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Required for the assembly of mitochondrial cytochrome c oxidase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the COX19 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375841.1">
        <property type="nucleotide sequence ID" value="XM_007375779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANN5"/>
    <dbReference type="FunCoup" id="G3ANN5">
        <property type="interactions" value="292"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANN5"/>
    <dbReference type="GeneID" id="18875403"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_71943"/>
    <dbReference type="eggNOG" id="KOG3477">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141947_2_0_1"/>
    <dbReference type="InParanoid" id="G3ANN5"/>
    <dbReference type="OMA" id="YLTCRME"/>
    <dbReference type="OrthoDB" id="372487at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051383">
        <property type="entry name" value="COX19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21107">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21107:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="113" mass="12816" checksum="AA7E055BE4F855C3" modified="2011-11-16" version="1">MASGAPGGGNFRSWTPTPPERGSFPLDHDHECSSQMFKYLECMKFTENKNAPNCRILAKDYLKCRMDHQLMEQSDWDSLGLVNLPGEAGITHDQLLKSKTNKDKLKSDEKKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANX9</accession>
    <name>G3ANX9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable endonuclease LCL3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Probable endonuclease lcl3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_153741</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LCL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375879.1">
        <property type="nucleotide sequence ID" value="XM_007375817.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANX9"/>
    <dbReference type="FunCoup" id="G3ANX9">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANX9"/>
    <dbReference type="GeneID" id="18870962"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_153741"/>
    <dbReference type="eggNOG" id="ENOG502S1U4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046484_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANX9"/>
    <dbReference type="OMA" id="IYHTPGG"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000035">
        <property type="entry name" value="Probable endonuclease LCL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302">
        <property type="entry name" value="EBNA2 BINDING PROTEIN P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="16">
        <location>
            <begin position="58"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW32603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="233" mass="26901" checksum="ABCCB7E4AAD335C6" modified="2011-11-16" version="1">MAPVTNTPPEVSILHPKVLLFTAGLTTSIFLSYRFYKRYIRRIRNYLDLTSSILNNRQSLYGYVTSVPDGDNFRFYHTPGGRLLGWGWLRSIPTTRAALKDETLMIRLCGVDAPECSHFGKPAQPYSQEALQWLREYINGRYVTITPYSIDQYKRVVARAQIWKWNGRKDVSAEMLKRGIAVVYEGKVGAEFGDNESWYRKLEAKAKKLKRGMWSLGKKLQTPSAYKKLYVNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANY7</accession>
    <name>G3ANY7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61672</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375888.1">
        <property type="nucleotide sequence ID" value="XM_007375826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANY7"/>
    <dbReference type="FunCoup" id="G3ANY7">
        <property type="interactions" value="1126"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANY7"/>
    <dbReference type="GeneID" id="18874003"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61672"/>
    <dbReference type="eggNOG" id="KOG0896">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063065_4_0_1"/>
    <dbReference type="InParanoid" id="G3ANY7"/>
    <dbReference type="OMA" id="GPESCSY"/>
    <dbReference type="OrthoDB" id="6508832at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031371">
        <property type="term" value="C:ubiquitin conjugating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006301">
        <property type="term" value="P:DNA damage tolerance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010994">
        <property type="term" value="P:free ubiquitin chain polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044395">
        <property type="term" value="P:protein targeting to vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23807">
        <property type="entry name" value="UEV_UBE2V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000026">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="5"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="137" mass="15515" checksum="2D6D793137B64F83" modified="2011-11-16" version="1">MSKIPRNFKLLEELEKGEKGLGAESISYGLTNQDDITMTYWNGTILGPPHSSHENRIYSLRIICDESYPDKPPKVTFVSKINLPCVDSQGNVITDEFDTLKNWKRSYTMETVLLELRKSMASPANKKLSQPPEGSTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3APC4</accession>
    <name>G3APC4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_61179</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375377.1">
        <property type="nucleotide sequence ID" value="XM_007375315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APC4"/>
    <dbReference type="STRING" id="619300.G3APC4"/>
    <dbReference type="GeneID" id="18873764"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61179"/>
    <dbReference type="eggNOG" id="KOG0252">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_12_1"/>
    <dbReference type="InParanoid" id="G3APC4"/>
    <dbReference type="OMA" id="IMFTNFQ"/>
    <dbReference type="OrthoDB" id="2261376at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001406">
        <property type="term" value="F:glycerophosphodiester transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000140">
        <property type="entry name" value="Putative MFS phospholipid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0843">Virulence</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="195"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="350"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="376"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="415"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="455"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="58"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="510"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="516"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="532" mass="59157" checksum="7374C46E2CDC48DE" modified="2011-11-16" version="1">MATRDLPHSVSDLLFGWSKHLKKEITLNKTQEQLIAEDFQNDEDAPPETKVKMTNLWPAFASGAGLFSDGYVNNSISTVISCLSIIYKDEFTKSNAISNIGSIAFVGTVVGQLSFGYISDRIARKGGMMTANIILIVFTLLCAVATWGKTPYGLFAAITTFRFFLGVGIGAEYPTSSVIASEFANMLPAGKRNRYFNWFTNFMIDTGFVISSFVPLVLLWIFSERHLRAVWRLTIGLGVIPPLSLFFIRLRMKNSSSFTKLHMKKVSYRNYPWWLITKFYWFRLTVISLMWFIYDFSAYSFGTYSTIIMGQIIPNDSLYKTWGWSVVFNLFYIPGAFTGAIAADYIGPRLTFVLGVGLQGVIGIIMSACLGSLRKHIAAFVVVFGIFTTLGEFGPGDNIGLLASKTSATPIRGQYYGIAAAIGKIGAFVGTWVFPVIQRRYADNADPNLELQVPFYISSALCIFSASIAFFFCPSVGQDAINREDEDFVNYLRANGFDVSQLGDGGVVEVQEESSDDNKEEKGRVNIDQESY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3APE5</accession>
    <name>G3APE5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_137985</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS25 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375398.1">
        <property type="nucleotide sequence ID" value="XM_007375336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APE5"/>
    <dbReference type="FunCoup" id="G3APE5">
        <property type="interactions" value="945"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APE5"/>
    <dbReference type="GeneID" id="18870088"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137985"/>
    <dbReference type="eggNOG" id="KOG1767">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129470_4_0_1"/>
    <dbReference type="InParanoid" id="G3APE5"/>
    <dbReference type="OMA" id="RIVHHSG"/>
    <dbReference type="OrthoDB" id="10263513at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004977">
        <property type="entry name" value="Ribosomal_eS25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12850">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03297">
        <property type="entry name" value="Ribosomal_S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="105" mass="11581" checksum="D44DA04334D9C7C2" modified="2011-11-16" version="1">MAPKVQQTKAAKAAAALAGGKKGKKKWNKGKVKDKAQHVVILDQEKYDRILKDVPTYKYVSVSVLVDRLKIGGSLARVALRQLEEDGIITPVLKHSKQAVYTRAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3APK9</accession>
    <name>G3APK9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Cyclin-dependent kinases regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_61266</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CKS family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375456.1">
        <property type="nucleotide sequence ID" value="XM_007375394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APK9"/>
    <dbReference type="FunCoup" id="G3APK9">
        <property type="interactions" value="572"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APK9"/>
    <dbReference type="GeneID" id="18873807"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61266"/>
    <dbReference type="eggNOG" id="KOG3484">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140546_0_0_1"/>
    <dbReference type="InParanoid" id="G3APK9"/>
    <dbReference type="OMA" id="RPINYGQ"/>
    <dbReference type="OrthoDB" id="440676at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061575">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.170.10:FF:000002">
        <property type="entry name" value="Cyclin-dependent kinases regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.170.10">
        <property type="entry name" value="Cyclin-dependent kinase, regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000789">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036858">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23415">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT/60S RIBOSOME SUBUNIT BIOGENESIS PROTEIN NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01111">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00296">
        <property type="entry name" value="CYCLINKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01084">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55637">
        <property type="entry name" value="Cell cycle regulatory proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00945">
        <property type="entry name" value="CKS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 5" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00068939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU311113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW32180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="102" mass="12276" checksum="335186CDB88B1AC1" modified="2011-11-16" version="1">MTKPRYLTDSERAKVLEFQDMIHYSPRYNDDTHEYRHVMLPKNMLKVIPQDYFNSETGTLRILLEEEWRGLGITQSLGWEHYETHAPEPHILLFKRPINYGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>G3APU1</accession>
    <name>G3APU1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Binds Sin3p in two-hybrid assay</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_137982</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375538.1">
        <property type="nucleotide sequence ID" value="XM_007375476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APU1"/>
    <dbReference type="FunCoup" id="G3APU1">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APU1"/>
    <dbReference type="GeneID" id="18870087"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137982"/>
    <dbReference type="eggNOG" id="ENOG502RBGV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012010_0_0_1"/>
    <dbReference type="InParanoid" id="G3APU1"/>
    <dbReference type="OMA" id="YDLVLCI"/>
    <dbReference type="OrthoDB" id="4064873at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051711">
        <property type="entry name" value="Stress_Response_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47540">
        <property type="entry name" value="THIAMINE REPRESSIBLE GENES REGULATORY PROTEIN THI5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47540:SF2">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="547"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="6"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="678"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="682"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="701"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="764" mass="88489" checksum="47B66B0B69458AB0" modified="2011-11-16" version="1">MRVSKACDRCRTQKIKCSGTHPCNTCVKHKKECTFSTNYHASAAGSTTTNTGFSANGKSTSDHPLNKRQKTVMNVEPFGLPQMDRNHDKEYVAHLENRVQYLESLLTQNMSQTFREPRISEPENVQVEETLFASSSKWRFSRRHQNLLIVELCRSMFANLSEESKKLVTIPRTQYFGWNMSGVHYVNSDELPPIPDFTNCPKFNPQELIDYYFRELNSLFAVLHETVFREQYQAYYKLLQEQQLNKSNDRDAKTNQTRLFSAILYLVYALAIRFSEVQKPKGPNIEMLQFESKLFKYGYKVVSILSFEWESFELIQAWLLIGLYLRVTHRQTSSSHAFDSAIVMTKSMGLGFEESNPKMLACTAYERVKANRIFWCVYTFDRIFGLLTGRYCGIREEDFDRRFPSYDDFKIDTFKDDWMTFPAMALLHVARIANFVHTAPNDNPDLIKYQQINTELAKLHDWFNSNGFRNDLLFNKEDDTISSLVKAQVKLHYYDLVLCIHGKILFNYIGRRITSHGLKIEMVIDASNGVLEVLDKVNKAGLLYTPWYSTLLLLFNVGVCALTLINGGVFVVQARDLLKNTIRLFTILRKSPVRNDQGKLVFRERFKMVRECIWALKMANRILTLRIQEDMTALNNIGVDHGSSEVNKQTFTQLGVNSTEVNKKTNNEFNKVFEKQLHRNDDEDEDEEDEEFRPRELKTPISPNQEHATPSSITSVDNSSAFGGVNSGDPIDFYVNNNGHDLLGNIQWFDQWMDFNYDFGEQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ10</accession>
    <name>G3AQ10_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Tricalbin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_153115</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375607.1">
        <property type="nucleotide sequence ID" value="XM_007375545.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ10"/>
    <dbReference type="FunCoup" id="G3AQ10">
        <property type="interactions" value="215"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ10"/>
    <dbReference type="GeneID" id="18870942"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_153115"/>
    <dbReference type="eggNOG" id="KOG1012">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001661_1_0_1"/>
    <dbReference type="InParanoid" id="G3AQ10"/>
    <dbReference type="OMA" id="VLMDDYM"/>
    <dbReference type="OrthoDB" id="1029639at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04044">
        <property type="entry name" value="C2A_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04052">
        <property type="entry name" value="C2B_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04045">
        <property type="entry name" value="C2C_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04040">
        <property type="entry name" value="C2D_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21678">
        <property type="entry name" value="SMP_TCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037761">
        <property type="entry name" value="C2A_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037765">
        <property type="entry name" value="C2B_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037762">
        <property type="entry name" value="C2C_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037756">
        <property type="entry name" value="C2D_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056910">
        <property type="entry name" value="TCB1-3_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017147">
        <property type="entry name" value="Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052455">
        <property type="entry name" value="Tricalbin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980">
        <property type="entry name" value="TRICALBIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980:SF2">
        <property type="entry name" value="TRICALBIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24920">
        <property type="entry name" value="C2_TCB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25669">
        <property type="entry name" value="SMP_MUG190-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037232">
        <property type="entry name" value="Tricalbin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="153"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="14">
        <location>
            <begin position="206"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="400"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="547"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="670"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="1012"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="66"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="834"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="16"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="852"/>
            <end position="866"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW32331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1211" mass="132357" checksum="91DD08169848F5DD" modified="2011-11-16" version="1">MATPQTNESGKSPDIGTGSVTGSVTGSVTGSVTGSVTRSATGTGTAAASVDQLSLNPTLDLKRKQSTKAAAELTPSEDLDISNVKTKPAPRKPIEPTFRGWKEVGKYEENDALTEEDENTDLLSKGSSIDSVLPPVIYGDWYHNAGYLIVGGLLSWIVGWFRFSIAPVFFIMVVFSILYRASVKKYRGVLREQAQREFSIKKIETDYESMDWANYFLEQFWAYLEPSISQIVCDQANPIMASNPYVPAFIKALWIDSFTLGTKPPRVECVKTMHGTADDVVVMDWGFSFTPNSLVDANFKQLKSKVNQKTVVRIKLFGVSIPITVSDVSCKGLARVRMRMMTSFPHVETINVSMIEPLDFDFNTKIGGESNFWWEVLSLPGLYPFINEMVKKYVGPMLFSPLSFQLNVQQLLSGNALNSAIGVLAITVDSARGLKGFSSIGNTLDPYLTFGFKSDVLAKSTTKSDTKAPVWNETFYLPVTSLSEPLHISVVDFNDFRKDREVGVILFDIESCVDNPKQSGLSAPFLRNNKPVGELSFAIQYMPTLEPIKQADGAVIPPPDLNTGIASITIEEAKQLRGGDKGVSAYAELKLNNDSVLKTNLIKNSNSPGWGSSTEEIIFNKAKTRVKVLIKDKNDRVIGQVNTNLNSLIDATQVDQTWFPLSKGGEVRITTNWKPVSIEGGSAGVGYAPPIGAVRISIKDAEDLINLETIGKVDPYAKILVNGVERARTVACESTLHPTWNEIHYATVTSPNQKLTIEVMDVEAHSPDRTLGSFDVKLTDIIQKDETGHYIEYIDTKPRTSKLIHKKGMKGYVHYSISFYPAIPVMSLSEIKEEEEEKKKAEQEAAAAAEAAKAEGKEPGAKPAAKEDDDEGFGDVMEAKEKLTLEQLIEHTSGVFVYEIIDGTLSKDDVFLQAFFDNNMFNNFVTTEIKRKKAKLGLTGDAVIPELEWSQATFRLVKNNDDNRAAKAIAETSIPTLQLLKNAYSKPTKIKLSGAGEATFTVQCSWIPLVYHAGIPPQDSINNAGILTVEVLRAEGLPAADSNGKSDPYVELFLNTEKKSFHKTKKVKKTLSPEWNESGEVEVANRYDSDIKLVCMDWDMAEKDDLLGTGYIHLKEYDTTRGPVEAEVPLFGENGESAGKAFVRLSFKTQFVMNVKARGTAAIGLTTVGSVGAGVGKGVTKGVGTVGKGIGSGIKGIRRGLRLGGGSSGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ27</accession>
    <name>G3AQ27_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Leukotriene A(4) hydrolase</fullName>
            <shortName evidence="16">LTA-4 hydrolase</shortName>
            <ecNumber evidence="16">3.3.2.10</ecNumber>
            <ecNumber evidence="16">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_139780</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Aminopeptidase that preferentially cleaves di- and tripeptides. Also has low epoxide hydrolase activity (in vitro). Can hydrolyze the epoxide leukotriene LTA(4) but it forms preferentially 5,6-dihydroxy-7,9,11,14-eicosatetraenoic acid rather than the cytokine leukotriene B(4) as the product compared to the homologous mammalian enzyme (in vitro).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>an epoxide + H2O = an ethanediol</text>
            <dbReference type="Rhea" id="RHEA:19037"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32955"/>
            <dbReference type="ChEBI" id="CHEBI:140594"/>
            <dbReference type="EC" id="3.3.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15 16">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 16">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 16">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.3.2.10" evidence="16"/>
    <dbReference type="EC" id="3.4.11.-" evidence="16"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375624.1">
        <property type="nucleotide sequence ID" value="XM_007375562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ27"/>
    <dbReference type="FunCoup" id="G3AQ27">
        <property type="interactions" value="1053"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ27"/>
    <dbReference type="MEROPS" id="M01.034"/>
    <dbReference type="GeneID" id="18870238"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139780"/>
    <dbReference type="eggNOG" id="KOG1047">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014505_1_1_1"/>
    <dbReference type="InParanoid" id="G3AQ27"/>
    <dbReference type="OMA" id="FPGNHHP"/>
    <dbReference type="OrthoDB" id="79562at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09599">
        <property type="entry name" value="M1_LTA4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000009">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.320:FF:000001">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2010.30:FF:000001">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.320">
        <property type="entry name" value="Peptidase M1, leukotriene A4 hydrolase/aminopeptidase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012777">
        <property type="entry name" value="LTA4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049980">
        <property type="entry name" value="LTA4H_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038502">
        <property type="entry name" value="M1_LTA-4_hydro/amino_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034015">
        <property type="entry name" value="M1_LTA4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015211">
        <property type="entry name" value="Peptidase_M1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02411">
        <property type="entry name" value="leuko_A4_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726:SF3">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09127">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01263">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 16 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 16" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 16" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 15 16" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M1 leukotriene A4 hydrolase/aminopeptidase C-terminal" evidence="17">
        <location>
            <begin position="465"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="13">
        <location>
            <position position="384"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="135"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="260"/>
            <end position="265"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="574"/>
            <end position="576"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR612777-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR612777-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR612777-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU361141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW32348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="620" mass="70410" checksum="C3E61F5EFA3AB8F5" modified="2011-11-16" version="1">MTSLIENIKSRSHELDPCTQSNYTQFQVKHTTLTLGVSFEDKVLTGTVVFELVNKSKTDSVILDTAALDISQVKVNSDVTPFKLAPAVPIYGSALHISIPTNDSTVKVEIDFRTTDKCTAIQFIAGDTGPYVFSQCQAIHARSLFPCFDTPGVKSTYTFTAKSPSPCTMSGRPRASSEAGVYHFDQPIPIPSYLVSLTSGNLLKAPIGPRSDVYCEEPNLKACQWEFEKDMENYIQIAEALIFDYEWDRFDSLVLPSSFPYGGMEIPNMTQLTPTLICGDRTQTKVMAHELSHSWSGNLVTNCSWEHFWLNEGWTVYLERRILGAIAAKERNSAEYGEQVRHFNMIIGWNGLVESVNGMPAKFTKLVWDLQGQDPDDAFSKIPYEKGFFFLFHLETKLGGLKEFDPFIKHYFKKFRYGSLNSAQFVETLYEFYEPLGKKQVLDSIDWDTWLFKEGVPEYPKFDTTLADQVYSLVDKWVAYVEKGGEFTFSEADVQSFEGEQEMLFLETLVEKFKQTQVTPELIRKIPSVYPKYSASGNGEFISRWNELLITFGKYSAGEEVVQKFATWLGSVGRMKYVRPGYKLLSSGVSLEFARETFAKYEQNYHPICHAMVKKDLQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ36</accession>
    <name>G3AQ36_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">60S ribosomal protein L18-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_61951</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376161.1">
        <property type="nucleotide sequence ID" value="XM_007376099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ36"/>
    <dbReference type="FunCoup" id="G3AQ36">
        <property type="interactions" value="995"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ36"/>
    <dbReference type="GeneID" id="18874119"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61951"/>
    <dbReference type="eggNOG" id="KOG1714">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080024_0_1_1"/>
    <dbReference type="InParanoid" id="G3AQ36"/>
    <dbReference type="OMA" id="IDICHKN"/>
    <dbReference type="OrthoDB" id="6353017at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.100.10.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000039">
        <property type="entry name" value="Ribosomal_eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021132">
        <property type="entry name" value="Ribosomal_eL18/eL18-A/B/_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17135">
        <property type="entry name" value="Ribosomal_L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01106">
        <property type="entry name" value="RIBOSOMAL_L18E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="4">
        <location>
            <begin position="4"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW31383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="186" mass="20640" checksum="37044DBE0D32BDC4" modified="2011-11-16" version="1">MGRDHTTKQHARSGHRAAPKSDNVYLKLLVKLYTFLARRTDAPFNKVILKSLFLSKINRPPVSVSRIARALKQKGSAEKTIVVVGTVTDDNRLLEFPKATVAALRFTAGAKATILKNGGEAITLDQLALRAPKGQNTLIVRGPRNAREAVRHFGFGPHKRKAPRILSKGRKFERARGRRNSKGFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ65</accession>
    <name>G3AQ65_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_140470</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376190.1">
        <property type="nucleotide sequence ID" value="XM_007376128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ65"/>
    <dbReference type="FunCoup" id="G3AQ65">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ65"/>
    <dbReference type="GeneID" id="18870294"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140470"/>
    <dbReference type="eggNOG" id="ENOG502RZW2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098356_1_0_1"/>
    <dbReference type="InParanoid" id="G3AQ65"/>
    <dbReference type="OMA" id="AFMWFLW"/>
    <dbReference type="OrthoDB" id="5423111at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032126">
        <property type="term" value="C:eisosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052649">
        <property type="entry name" value="NCE102-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28165">
        <property type="entry name" value="NON-CLASSICAL EXPORT PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28165:SF1">
        <property type="entry name" value="NON-CLASSICAL EXPORT PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="6">
        <location>
            <begin position="6"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="170" mass="18172" checksum="69E45D3B6AB85622" modified="2011-11-16" version="1">MLAIGDAILRLVNFLFLVIALGLTGSLSATTVDGQNPQVNFAVFAAAFGLLTSSIYGVFAYFINAFAWPVILFVFDFLNFVFCFAAATAIAAGIRVHSCSNEDYVDNNKITQGSSGRCRKAQASVAFLYFSTFIFLASGIFSAISLFQGGLFGANRPSARRTGVPTMSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQN3</accession>
    <name>G3AQN3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1765-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_154950</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376358.1">
        <property type="nucleotide sequence ID" value="XM_007376296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQN3"/>
    <dbReference type="FunCoup" id="G3AQN3">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQN3"/>
    <dbReference type="GeneID" id="18871003"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_154950"/>
    <dbReference type="eggNOG" id="ENOG502QPNW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005190_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQN3"/>
    <dbReference type="OMA" id="WEFWLGC"/>
    <dbReference type="OrthoDB" id="296767at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013887">
        <property type="entry name" value="UPF0592"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37988">
        <property type="entry name" value="UPF0592 MEMBRANE PROTEIN C7D4.03C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37988:SF1">
        <property type="entry name" value="UPF0592 MEMBRANE PROTEIN C7D4.03C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08578">
        <property type="entry name" value="DUF1765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="768"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="814"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="768"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="814"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="848"/>
            <end position="893"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1103" mass="124709" checksum="D52C48E13EB04378" modified="2011-11-16" version="1">MAILLIKPTRSRTKSLRASDIGFSNIKAAPTTPSTIQSAQDTTPRYYSSPSNASFRLDAASAAAAAAAVANTTTPTVTRSNSANSISSPDTDKLIKSLLKQFKKLEVELHKFNSRKFNHNNNGVILKGNILRTSLLPFLRTANQLNEYFSKDSPIYISLTSVIVSILIKWWNSLIGNLVLTPSASATSSTASTSAHVETIHYSNISASDRNAYLECISRIISREEWKYYQDDTNDYNALLIRTLDYCIDKMSTFKTLSASFSAFIGKVFAYSFFKLPNVSNALLFLLNVKQITFETCMKRLTGFERTSIDKSLFAQHLHSILNYNGISTGLVTKGQKSCMNCMPTPKHPVKGIKDPNGDWVRRWCCSDSNVFNSFFRHYIDIIQKLMEEEDKNVDLISCPGFTVIITHVFQILQVAVARISNNYPNPTKGPIHAQTTNLSPKQQLRGTSDKILPPLPPPVSFNINMKQSDIYYNSFIKIFKTIRDIAYCATLNEKSIDGATASLVRMIDLCLISMAKETSVYDFNKNGLIMSIANEFINHIVNNNPTNEVSYLINWEFWLGCNYMMIKHSDHVQIILKNFAFLFNIWDMIPETLSQLVKLGDGKVSISQTEEPYKWLTNLEESFKVNFINFLTGSEMFQRFFCHWNPVIRSYYIRLLVWRIIGVNNYQSSTMIRVTRQVQMKLNQAFDVLHEFTNANNGKFEMNYKSDNPLVNRRFGILPINVRDDYLSINEESSPDFLPPATLKSSELKKTHPYEVFDEAIYTCSSSVPPEVPRSSSAGSLSSITSRPKSSSNPIVSSLGKLLRMLSVDDNNSNQLNDIQENGTFDSTTPDSKRKLSFSPPQMRRNSVSLTSLSSTYSSLKSRSSSPSMSSYKSTTTVTDSTSSSIQSDSESLTSIDTIKTASLGKLLQQQQQQLLQSYNIPPPELSRLPPDIVRPIYKFDIVVDHESLNEKYNLIQQKNALVASGIVLNGAPCQPPQISYFPMPPQLPFISIFINSESFSSNFYINEEDSLFFENYETDVSEDEVVFDRSLINMVNLGKSLNELNMIIEEFRRFLNARIEVDSFNMEYFNQNELNEFVYFKKIIPFLSIDSSNELKLLNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARF3</accession>
    <name>G3ARF3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_51300</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376052.1">
        <property type="nucleotide sequence ID" value="XM_007375990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARF3"/>
    <dbReference type="GeneID" id="18871542"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51300"/>
    <dbReference type="eggNOG" id="ENOG502S48Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092522_0_1_1"/>
    <dbReference type="InParanoid" id="G3ARF3"/>
    <dbReference type="OMA" id="RINSGNT"/>
    <dbReference type="OrthoDB" id="5398391at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3330.10">
        <property type="entry name" value="Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018020">
        <property type="entry name" value="OHCU_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036778">
        <property type="entry name" value="OHCU_decarboxylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37987">
        <property type="entry name" value="CHROMOSOME 9, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37987:SF1">
        <property type="entry name" value="OXO-4-HYDROXY-4-CARBOXY-5-UREIDOIMIDAZOLINE DECARBOXYLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09349">
        <property type="entry name" value="OHCU_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158694">
        <property type="entry name" value="UraD-Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase" evidence="3">
        <location>
            <begin position="13"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="86"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="175" mass="19837" checksum="1F24AE353A1768B7" modified="2011-11-16" version="1">MTYTLLPIEYLSSLSREEISTTLGHLFEPCETLEQLIATSVIPHKEKYHTYSQFIELVRTELIQSLKTLDNDAINAIISAHPRLGDSTTSTHSTNEQKSLGGETEREKLKKLNELYEQTFPGLRYVVFVNGRSRDVVMQDMKQRIASSDIDLEKRLAFDAMCDIALDRAKNLGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARH1</accession>
    <name>G3ARH1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitochondrial protein PET191</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_154223</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PET191 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376070.1">
        <property type="nucleotide sequence ID" value="XM_007376008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARH1"/>
    <dbReference type="FunCoup" id="G3ARH1">
        <property type="interactions" value="276"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARH1"/>
    <dbReference type="GeneID" id="18870974"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_154223"/>
    <dbReference type="eggNOG" id="KOG4114">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138069_1_0_1"/>
    <dbReference type="InParanoid" id="G3ARH1"/>
    <dbReference type="OMA" id="CIANFRA"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018793">
        <property type="entry name" value="Cyt_c_oxidase_assmbl_Pet191"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28627">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28627:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10203">
        <property type="entry name" value="Pet191_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14735" checksum="11B8A8FF971FA5B0" modified="2011-11-16" version="1">MGASCKDQKKALAICLQRSPCVLIERNTPKKCLTDPELKKELPELCVHNFMAYMECRHGFFDMRKRMRGNAPMSTGKYDDVYDHLSSGDFDAHEEMKKLDILNRNLSKQRQLKEEQERLRLSGELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARJ9</accession>
    <name>G3ARJ9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Stress response regulator protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_56192</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Required for stress adaptation, morphogenesis and virulence.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376098.1">
        <property type="nucleotide sequence ID" value="XM_007376036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARJ9"/>
    <dbReference type="STRING" id="619300.G3ARJ9"/>
    <dbReference type="GeneID" id="18872013"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56192"/>
    <dbReference type="eggNOG" id="KOG0519">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065405_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARJ9"/>
    <dbReference type="OrthoDB" id="303614at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036180">
        <property type="term" value="P:filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900445">
        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050595">
        <property type="entry name" value="Bact_response_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591:SF3">
        <property type="entry name" value="RESPONSE REGULATORY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591">
        <property type="entry name" value="STRESS RESPONSE REGULATOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="111"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00037668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00040436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW31320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="240" mass="27974" checksum="AA636CB20D38C024" modified="2011-11-16" version="1">MFGAEYFNPFQKILISSDTPISSSTQPINHNNSIPKRASFNIDHKLSIEITSEKSDENYTTPQPYNYDYQYITSHSTYSPITPEDLFPPPVINSYFPSEENVVIPQFSPYRFLVVDDNIVNLQILSRQLLKIFPYAEINQIQDSTKVKQLLDRNTFDVVFLDIEMPEVDGIELAQYMRCNEKFNQWGVIAVTTLTTTRDIQRYKQCGIDYTFKKPMNADLNHIARVVDEIIRQRKYGRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARN5</accession>
    <name>G3ARN5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_62383</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376566.1">
        <property type="nucleotide sequence ID" value="XM_007376504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARN5"/>
    <dbReference type="STRING" id="619300.G3ARN5"/>
    <dbReference type="GeneID" id="18874299"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62383"/>
    <dbReference type="eggNOG" id="KOG4304">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033003_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARN5"/>
    <dbReference type="OMA" id="EMNETEN"/>
    <dbReference type="OrthoDB" id="690068at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00083">
        <property type="entry name" value="bHLH_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="80"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="245"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="427" mass="47324" checksum="68ADFB7359BC80B6" modified="2011-11-16" version="1">MVFPSPPVLKTTIIQLNKELDSDDKNYEILTNEYTNNSTNNSVDIINSGSGSSSLSNNKKKRRRSSANIDSEELAKRKHETKQLHSIIEKRRRIKINREFEALKYLIPACRASNANQPTATAKKNQPNPGNANGNKIDGMYKLTILKSSVEYILYLHHIIQKQHELLQQSNNEAYSKFNIDFAKIPLDVNQYRNIDKEFNFKELLEGTSPSTSSSEMSSSGRSFDYRTHSIVEEQEEDDEGNTSSTSTATINGPTTTNTPTNYQSTIPMSASSSISELKKPQLPSPEFTPDIAPILTMLNKYQTASSASQSPNLISRKSSYASPNMLGIKSNNPSPFTIPMKSTLSGSAFTSPSMIGSSSSSAKANGFRLPEPAVEVDQQLVPPRKMYFKSKSNASFAKEDSYEDKDQDASKTLLALRKSSIDSLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARS5</accession>
    <name>G3ARS5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA ligase</fullName>
            <ecNumber evidence="14">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_56206</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="14"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376120.1">
        <property type="nucleotide sequence ID" value="XM_007376058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARS5"/>
    <dbReference type="FunCoup" id="G3ARS5">
        <property type="interactions" value="686"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARS5"/>
    <dbReference type="GeneID" id="18872015"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56206"/>
    <dbReference type="eggNOG" id="KOG0967">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005138_4_1_1"/>
    <dbReference type="InParanoid" id="G3ARS5"/>
    <dbReference type="OMA" id="WIKYKRD"/>
    <dbReference type="OrthoDB" id="206088at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035753">
        <property type="term" value="P:maintenance of DNA trinucleotide repeats"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903461">
        <property type="term" value="P:Okazaki fragment processing involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07900">
        <property type="entry name" value="Adenylation_DNA_ligase_I_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07969">
        <property type="entry name" value="OBF_DNA_ligase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3260.10:FF:000001">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000062">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000016">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF4">
        <property type="entry name" value="DNA LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="17">
        <location>
            <begin position="457"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW31342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="715" mass="80312" checksum="EB6CADD351BA6367" modified="2011-11-16" version="1">MAPQQQSIARFFGGGATLAKKKDLNPSSPTKKRPLSPPPPTASPDKRTKITPETAVKVGESEIHEDTDPEIDEPVKPKSQLDEAQELIKLSKEHQHDKPVVVSSKIPYATLTSILEKIENESSRLKIISILSQFFEDILSASSIDKLIKIVYLVINRLGPDYEPDLELGLGETLLIKAISEGYGRATSKIKSDYKEVGDLGLVAQKSRSGQPTMFKPQPLDIDTVFANLTQIARSTGKDSQSKKIGIINKMLTACDPKTNEAKFLIRSLEGKLRIGLAEKTVLIALAQAFVNHEKKHKVHADELSKAEDIIREAFSQIPNYEVIIKNASEHGVFNLLDHVRLTPGIPVKPMLAKPTKSIGEVLDRFQHEEFTCEYKYDGERAQVHVLPDASVRIYSRNSEDMSQRYPDLISIITEFSKQQELHSMILDCEAVAWDRVQNKILPFQVLSTRKRKDVNEKDITVHICLFAFDLIYYNGESLITKSLAERREVLYNNFTTIEGKFQFATKKDSSNLDELQLFLDQSVKDSCEGLMVKMLNGTESFYEPSKRSRNWLKLKKDYLAGVGDSLDLVVIGAYNGRGKRTGTYGGFLLASYNQDSGELETTCKIGTGFSDEDLTNLYNKLHPTEIPNPRPHFVYDSTNSNAAPDVWFEPSLLFEVLTADLSLSPIYKAAHTEYGKGISLRFPRFLRIRDDKGIEDATSSTEVAEFYERQAHVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARW7</accession>
    <name>G3ARW7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_56576</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376594.1">
        <property type="nucleotide sequence ID" value="XM_007376532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARW7"/>
    <dbReference type="FunCoup" id="G3ARW7">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARW7"/>
    <dbReference type="GeneID" id="18872049"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56576"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016304_6_3_1"/>
    <dbReference type="InParanoid" id="G3ARW7"/>
    <dbReference type="OMA" id="VEMNGAW"/>
    <dbReference type="OrthoDB" id="446113at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061158">
        <property type="term" value="P:3'-UTR-mediated mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12344">
        <property type="entry name" value="RRM1_SECp43_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000463">
        <property type="entry name" value="Nuclear acid binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050825">
        <property type="entry name" value="RBM42_RBP45_47-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640:SF10">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442570" description="RRM domain-containing protein" evidence="5">
        <location>
            <begin position="21"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="94"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="212"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="388"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="19"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="299"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="513"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="517"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="554"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="586"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="603"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="632" mass="70119" checksum="197F247BF378391A" modified="2011-11-16" version="1">MLHTLKLLALFSVMFTTTTTSSTAPDQSTSTPTTVPKITINPNATTSNTASHQHQQEQEMLSSFTVPNPPVSTQQSSAVVAADNNTDSDYERPRTLWMGDLDPWLDEQGITDLWWNILHKRVVVKIIKPKSSISNLDPNYQGLTNSGYCFVEFETFEDAQQALSLNGQLLPDIAMPSQQHFPNNPDNQKKYFRLNWASGATLTAPIIQTPEYSLFVGDLSASTTEAHLLAFFQKSFPNSIKTVRVMTDPISGKSRCFGFVRFTDESERQRALVEMNGVWFAGRPLRVALATPRTNPRNKYEMMFIPPPPPPPPGSGQLPGQPIYPPNYYTQAQSQQQSQSQQPSHQQLQQQYDFPQQDIDVNLDMMMKSPLAPMHHQLAQPYADPTNTTVFVGGLSSEVSEPTLFTLFKPFGIIQQVKIPPGKNCGFVKYTTREEAEEAIAAMQGFIIGGNRVRLSWGRVSPTNKKYQLQQQQQQQQHQHLQQSQVQQQAQAQAQQQQMQAAAAFSMGIDPGLMGYPPVPGPVPPGPPPPPGTIPGSGQVPPPPPGATMGMMFQESSSGEYESRSNMSPPFFIPMHGPPGHGPDANLDEDKNEEQQEGDSNNEQDKKEEHQGNDEEKSGDSDEKKSDEEKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS35</accession>
    <name>G3AS35_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_56624</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion inner membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376663.1">
        <property type="nucleotide sequence ID" value="XM_007376601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS35"/>
    <dbReference type="FunCoup" id="G3AS35">
        <property type="interactions" value="543"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AS35"/>
    <dbReference type="GeneID" id="18872055"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56624"/>
    <dbReference type="eggNOG" id="KOG3489">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141397_1_0_1"/>
    <dbReference type="InParanoid" id="G3AS35"/>
    <dbReference type="OMA" id="NEICWDK"/>
    <dbReference type="OrthoDB" id="6417021at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042719">
        <property type="term" value="C:mitochondrial intermembrane space chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140318">
        <property type="term" value="F:protein transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="3 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0811">Translocation</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Tim10-like" evidence="8">
        <location>
            <begin position="22"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="89" mass="9975" checksum="5D8CC697461743E1" modified="2011-11-16" version="1">MSSLNAAALQNLDEPSRKEIMQFIESEQSKSKVQSSIHGFTDMCFKKCFKDVPITNGSLTSNEESCLKNCLNRFLDTNIKVVEALQTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASC3</accession>
    <name>G3ASC3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sorbose reductase SOU1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_62532</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376696.1">
        <property type="nucleotide sequence ID" value="XM_007376634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASC3"/>
    <dbReference type="STRING" id="619300.G3ASC3"/>
    <dbReference type="GeneID" id="18874364"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62532"/>
    <dbReference type="eggNOG" id="KOG0725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_1_1"/>
    <dbReference type="InParanoid" id="G3ASC3"/>
    <dbReference type="OrthoDB" id="1888931at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050085">
        <property type="term" value="F:mannitol 2-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000090">
        <property type="entry name" value="NADP-dependent mannitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF13">
        <property type="entry name" value="L-XYLULOSE REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="287" mass="31217" checksum="A5C6B40D2B4C4259" modified="2011-11-16" version="1">MAPTTTQETPIVSYISEEFTDELPRESPPKRHIMDLLSLKGKVAVVTGAYRGIGYAIAETYAEAGATVILVDYVDCTNEAVKLGLQHKVIAKAYQCDVSKSYDVDATIEAIEFDFGTIDIFVANAGIIWKTGNIVAEVNQDGSTWHKLMDVNLHGVYYCAQSIGKIFQRKGKGSFIITASMSGTIANVPMNLTPYNVSKAAVKHFAKSLAVEWAGFARVNSISPGYCDTGLNDHLSREYRGKMWSLIPAGREALPYEIASAYLYLASDAASYVTGSDFIIDGGYTCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASX3</accession>
    <name>G3ASX3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Carnitine O-acetyltransferase, mitochondrial</fullName>
            <ecNumber evidence="16">2.3.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">CAT2</name>
        <name evidence="21" type="ORF">SPAPADRAFT_62609</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Carnitine acetylase is specific for short chain fatty acids. Carnitine acetylase seems to affect the flux through the pyruvate dehydrogenase complex. It may be involved as well in the transport of acetyl-CoA into mitochondria.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(R)-carnitine + acetyl-CoA = O-acetyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:21136"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57589"/>
            <dbReference type="EC" id="2.3.1.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.7" evidence="16"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376788.1">
        <property type="nucleotide sequence ID" value="XM_007376726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASX3"/>
    <dbReference type="FunCoup" id="G3ASX3">
        <property type="interactions" value="198"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASX3"/>
    <dbReference type="GeneID" id="18874402"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62609"/>
    <dbReference type="eggNOG" id="KOG3717">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013513_5_1_1"/>
    <dbReference type="InParanoid" id="G3ASX3"/>
    <dbReference type="OMA" id="KMDGTPT"/>
    <dbReference type="OrthoDB" id="240216at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004092">
        <property type="term" value="F:carnitine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000007">
        <property type="entry name" value="Carnitine O-acetyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF103">
        <property type="entry name" value="CARNITINE O-ACETYL-TRANSFERASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00439">
        <property type="entry name" value="ACYLTRANSF_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00440">
        <property type="entry name" value="ACYLTRANSF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 19" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="20">
        <location>
            <begin position="51"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00066910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EGW30755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="640" mass="72330" checksum="951D939D745668E6" modified="2011-11-16" version="1">MIKLRINQLVSVTSKATSTKSIMSILTSAKRYNSSQPKGDLYKFQSQLPRLPVPALQDTAAKYLKSVEPFLSPAQLAETTAKVQDFIKPGGVGETLQSRLTQFAQGKDNWLAEFWDDYAYMSYRDPVVPYVSYFFSHKDIRGVVGENQLLKASLIAYYTIEFMEQVESEQLPAEVVKGTPYCMNAFKYMFHNSRVPAPGSDITKHYDGQENQFFVVIYQNNFYKVPTHDANGNKLNKGEIYQYLQQIKNDATPPGIGLGALTSLNRDEWLSAYNTLRKSPVNEASLETIFASAFVICLDSYNPITIEEKSRNCWHGDGQNRFFDKPLQFFIGANGNSGFLGEHSRMDATPTVQLNNTVYKQVIAENENSNLVEEVATFLPRVGKAEIIPFDIDPVSRTNIVEAIVKFEETITAHDEEIFQYYGHGKNLIKKFKTSPDAYVQMMMQLAYYKLTGKVRPTYESAATRKYLKGRTETGRTVSNESKKFVETWTNPTATIADKVATFQAACKQHVSYLSSAADGQGVDRHFFGLKNMLAQGEPVPEIFTDPIFSYSSKWYISSSQIPSEYFQSWGWSQVIDDGFGLAYLINNEWIHVHISCKRGNGLSSDHLKWYLVESANEMKDVLSKGLLEGKVEEKSKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASX4</accession>
    <name>G3ASX4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RFX-type winged-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_155956</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EGW30756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="STRING" id="619300.G3ASX4"/>
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    <dbReference type="eggNOG" id="ENOG502RQH9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009415_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASX4"/>
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    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003150">
        <property type="entry name" value="DNA-bd_RFX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02257">
        <property type="entry name" value="RFX_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51526">
        <property type="entry name" value="RFX_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RFX-type winged-helix" evidence="2">
        <location>
            <begin position="295"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="425"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="426"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="859" mass="98046" checksum="F918504EFA2702A7" modified="2011-11-16" version="1">MRTSPVVTTATPGSYYYQPPEQATFTGLQQQHRPQQPQQLQHQHSSSTTYGYNPQQPITSAFPSFDYSGAFKIPEYATAPIPQQLPPSRVLSSSTPQQQQNYPVPSQSSILRNVTTSLSPAAAAVFRQHQKNNLSISSHFNLVSLSPGDKRPGSNLGASRVVTQQHSSDNSVINDLLINLTSVDGSNINNYLLSILYKLNTPLPVDDFYNLLYNNDRLRQLLDINYHQKLDKTMVPYSNCEFSIDVINQLLNVFKNPNLLMEYLPSMEDKENKLLNINYHELLRTFLAIKILYDILIQLPLGAEEDPQSYTIPRLSIYKTYFIICQKLICNYPSSSNTTNEQQKLILGQSKLGKLIKLVYPNLLIKRLGSRGESKYNYLGVIWNENIVNDEIKQLCDQNELNDLNEIFSGDTRMSNQLLGATMKRTGTHRRNSSKHKRMAPSIPHLASDSNQPLSSPNLSSIKPLLKFPAGDNFSILTSEEEDNWFNQVLFECYNRIGIFGPNLIHEIFLKPENLVSNSSLLHNLMESLIKPLNVQTTIPNVDLCLYLVILIEISPYLLLIKSSTNISLLKTLRLNLMHLINNLNSELKLLDSEKFPINNSTIFLIMIKKLINLNDLLITFIKLINKDSRKSMMSTDIENFLKINSQTLKLEDQDDENLLFNLNTNLTTTSMGEINFSFRNDILSNDLVYTLIGYNFDPNINNELKSSISMKFINEEINIIDDFFKNDLLEFLNSSFSSNEEAKDEEESGEANEAVLSSKEFSRLQALINLIDKRLLSQHFKSKYPILIYNNYITFILNDILKYIFLKQQQSQLSNMQRSSQDLEGGSNNSFGNWWVFNSFIQEYMSLMGEIVGLHDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATB4</accession>
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            <fullName evidence="4">DUF1753-domain-containing protein</fullName>
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        <lineage>
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            <taxon>Saccharomycotina</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="GeneID" id="18875197"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68109"/>
    <dbReference type="eggNOG" id="ENOG502QT2Z">
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    </dbReference>
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    <dbReference type="InParanoid" id="G3ATB4"/>
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    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:inositol phosphoceramide synthase regulator activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006673">
        <property type="term" value="P:inositol phosphoceramide metabolic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Kei1"/>
    </dbReference>
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        <property type="entry name" value="INOSITOL PHOSPHORYLCERAMIDE SYNTHASE REGULATORY SUBUNIT KEI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28077:SF1">
        <property type="entry name" value="INOSITOL PHOSPHORYLCERAMIDE SYNTHASE REGULATORY SUBUNIT KEI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08552">
        <property type="entry name" value="Kei1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="26184" checksum="A4F8C2B350EEC942" modified="2011-11-16" version="1">MAVTITKNTIRVLPQKFLGRFPLFIGAEIILGITILNKASGIYGILSLLTGHPINFFQWLFNALALVLLPIYISAFININTQPKNVRKMSIATVVYMADTVIGLLYTLYFMYFWFYVEDTAAPESGNTKRQEMGDLSAQSASYTRELFLTFATTIVVKAVRIYFTLVMMSFTRALLKQHILREGRYGQDDAEHLVDADEEEILGSVGVLGEIRKTILDLEIKSKELLIQLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATF2</accession>
    <name>G3ATF2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nuclear cap-binding protein complex subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">80 kDa nuclear cap-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_52107</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NCBP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376948.1">
        <property type="nucleotide sequence ID" value="XM_007376886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATF2"/>
    <dbReference type="FunCoup" id="G3ATF2">
        <property type="interactions" value="1026"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATF2"/>
    <dbReference type="GeneID" id="18871628"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52107"/>
    <dbReference type="eggNOG" id="KOG1104">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011380_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATF2"/>
    <dbReference type="OMA" id="RIWVHQP"/>
    <dbReference type="OrthoDB" id="10252707at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005846">
        <property type="term" value="C:nuclear cap binding complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000339">
        <property type="term" value="F:RNA cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Sto1p"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027159">
        <property type="entry name" value="CBP80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015172">
        <property type="entry name" value="MIF4G-like_typ-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015174">
        <property type="entry name" value="MIF4G-like_typ-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412">
        <property type="entry name" value="CAP BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412:SF2">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09088">
        <property type="entry name" value="MIF4G_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09090">
        <property type="entry name" value="MIF4G_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MIF4G" evidence="12">
        <location>
            <begin position="48"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00074671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW30915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="933" mass="106964" checksum="42C86B790C5CC360" modified="2011-11-16" version="1">MDVNPAKRTRDEYERDHEDTHTHTAPTSALDESFSTKRQHVDPTTELINNVCKDIRRLGENPNIANQIDDIAYISNPIVAEFEKIDELRSAILDTVYAVIIEQPHKIHALANLVLVCNSKNFVVAKYVVEFLHAKVQGLLDSVGQEVEIKEGFDKEGAGVFNDIKCVLKFFSSLAPIIEGYGIVNIFSQLLQFAIDLQESTPKRHGIAQEIYYNVLISVPYLLSNDKSPEILEKIDSIIELAKKFNIVETESVLLQPFDSRLGNYELPYVPKKLINLILPSLSTLQESKWEDFKLFIDFAPFLDPIIEEALKNNTISNDIVKHSLPQFSLPSVETILKYSPHVNSIDRLWKHNSRLLFQVYNNSTDFETVPRIETYLGLFFKDIAFDILTNLSFNKNEAAIQLSILDLFYAKDLFTAPGSSIDQLAEVNKANQAGENTPALSTWKIEDVAVESILTMIFQLPTPLHPEIYYYTVLIACCRESPESIAPVFGRAIRFFYNHLETLDYELKVRFLDWMTTQISNFEYSWKWDEWVGDSERLAGLKYHPRRNFIKNLIAKEIRLSNKSRIKDSFVSMVQNRPISTTTGDVSDEEVEDADSNAADAGTTIVHLSEFNQYLDISMMAPNSEQYVLDYDVELYGTDEVKDTLIKLYEQKKEQIKGKSLVTAQDEIIYNFANPDLPYNESATKVYEFVVAHWKTNDEFSTLCQDILTSLESHPNPMQFLVNLVFQTYAYIGSRSIYSVVSILSRDINKLKHLSGATITYANDEPHFDTPELTPEEVTLRQQWIIDAIFRVWIHQPQVVFLILEYLIEFGIVDAKHIVTKALESNLIIDNVSCMESVNRILGSANKELIIVLFNQIVTNLNKVQFGDKIEEDSDDQWLFVEYLGLLKSYLRKYIKNNTKVDYIDELKELFGAVENAKAKEEILQWVDECDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATG4</accession>
    <name>G3ATG4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Phosphotyrosine protein phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_56873</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the low molecular weight phosphotyrosine protein phosphatase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376960.1">
        <property type="nucleotide sequence ID" value="XM_007376898.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATG4"/>
    <dbReference type="FunCoup" id="G3ATG4">
        <property type="interactions" value="609"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATG4"/>
    <dbReference type="GeneID" id="18872074"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56873"/>
    <dbReference type="eggNOG" id="KOG3217">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071415_2_0_1"/>
    <dbReference type="InParanoid" id="G3ATG4"/>
    <dbReference type="OMA" id="YQQVTRF"/>
    <dbReference type="OrthoDB" id="3388at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd16343">
        <property type="entry name" value="LMWPTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000105">
        <property type="entry name" value="Low molecular weight phosphotyrosine protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050438">
        <property type="entry name" value="LMW_PTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023485">
        <property type="entry name" value="Ptyr_pPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036196">
        <property type="entry name" value="Ptyr_pPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017867">
        <property type="entry name" value="Tyr_phospatase_low_mol_wt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11717:SF7">
        <property type="entry name" value="LOW MOLECULAR WEIGHT PHOSPHOTYROSINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11717">
        <property type="entry name" value="LOW MOLECULAR WEIGHT PROTEIN TYROSINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01451">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00719">
        <property type="entry name" value="LMWPTPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00226">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52788">
        <property type="entry name" value="Phosphotyrosine protein phosphatases I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphotyrosine protein phosphatase I" evidence="9">
        <location>
            <begin position="12"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR617867-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="173" mass="19894" checksum="A6B94CC505251EA2" modified="2011-11-16" version="1">MAPLFTPQEKQISVAFVCLGNICRSPMAEAIFKHKVQQLGYADYFNRIDSYGTASYHVGESPDRRSAATCRKHGVPVNHRAQQIRPNDFRSFDYVIAMDQSVLDDLRYMKPRDSKAVVELFGHWNDETGRKIMELSDIVEDPYYGGIHGFEHNYNQICYFSDQFLNQEVGSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATH9</accession>
    <name>G3ATH9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetylglucosaminylphosphatidylinositol deacetylase</fullName>
            <ecNumber evidence="2">3.5.1.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_141689</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIGL family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.89" evidence="2"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376976.1">
        <property type="nucleotide sequence ID" value="XM_007376914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATH9"/>
    <dbReference type="FunCoup" id="G3ATH9">
        <property type="interactions" value="547"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATH9"/>
    <dbReference type="GeneID" id="18870391"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141689"/>
    <dbReference type="eggNOG" id="KOG3332">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034979_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATH9"/>
    <dbReference type="OMA" id="YVLESVN"/>
    <dbReference type="OrthoDB" id="6220758at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000225">
        <property type="term" value="F:N-acetylglucosaminylphosphatidylinositol deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993:SF11">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="302" mass="34620" checksum="756E02D046068651" modified="2011-11-16" version="1">MIFTIPRAIFRFFALSFIIWLVLTTILPQTTMKYSNNQVRTNKFRRSQYPYNSITSPTPLPIANSSVYFIIAHPDDEVMFFSPSVVEISKSKYNNSVKLVCFSRGDAVDESMGRIRRNELYDSARILGVQDVTVLNYPDGMNETWALSDIRQSLKENVKPVEGKPTVLITFDEGGVSSHANHIALYHGTKSYFKRNGNATSTRLLVLKSVNFWEKYSFTLLTDVELFVDHLSSFISKVVNFNINVSFFTAAPSSSIKIYSDLNMLSVSYAAMCYGHFSQMVWFRYGWLLLSRYLTFNHLIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATQ9</accession>
    <name>G3ATQ9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_63131</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377255.1">
        <property type="nucleotide sequence ID" value="XM_007377193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATQ9"/>
    <dbReference type="FunCoup" id="G3ATQ9">
        <property type="interactions" value="140"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATQ9"/>
    <dbReference type="GeneID" id="18874608"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63131"/>
    <dbReference type="eggNOG" id="KOG2740">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026449_1_0_1"/>
    <dbReference type="InParanoid" id="G3ATQ9"/>
    <dbReference type="OMA" id="LNEMCDG"/>
    <dbReference type="OrthoDB" id="870at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09252">
        <property type="entry name" value="AP-3_Mu3_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="7">
        <location>
            <begin position="213"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="407"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="489" mass="55146" checksum="159E493F9DE2EAE1" modified="2011-11-16" version="1">MIEALYIADSTNTLVYEYSNSLTSPQFKTLLPKITAATSSTDIHQTNDIIPLNSKFHLLIHHESSLIFYVLHQYSQDVNPLFPTIFVRRLIETIRDYFGDLKTPSKIEANNDTLTLLLYQMLDDTIPYYTDFNKLRDLVAYKSLLSKFLSSASSTASKATAALKDTTGISSAKEESDIPWRRSNVRHTNNEMYVDVIETVSVILKPIPKKVGAQQFDSAFYSTTKAAAAQENYMISGTIDGEVNFVSRLTGVPLLQLIFNTLGVKDITFPSFHRCVNLDTWRERRGVLSFIPPDGKSTLMKYQIDLDSQYTSNSQRMGLLKQSTIDVDFICRENSNEFEIRLHFGMQVTKVESVTVEIVCQDPEDQIKLNRITHGDFGFKGNGKGEWNLRQLKSGISPVFHGSVISDLHKDGDKDQDDSEEKSESPEKPQAKYTKPSYIRVHYLHKGSVPSGLKVDNLKIISAKGLGDTVKPYKGVKYITKSGNYIIRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATR6</accession>
    <name>G3ATR6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_143614</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377264.1">
        <property type="nucleotide sequence ID" value="XM_007377202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATR6"/>
    <dbReference type="GeneID" id="18870557"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143614"/>
    <dbReference type="eggNOG" id="ENOG502R1XK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2348024_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATR6"/>
    <dbReference type="OMA" id="QFTENDH"/>
    <dbReference type="OrthoDB" id="4016934at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="97" mass="11234" checksum="F774876242431CB6" modified="2011-11-16" version="1">MNSSTTHHSKHVKIRRDKSTFTVSITLATDALEAIRLAQVKVKSPTKRQRNASTRSSQIEQYNQLVMTEQRRLRYTKRIQGIDLSPTKPSKHVRFTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATU6</accession>
    <name>G3ATU6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_63178</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377293.1">
        <property type="nucleotide sequence ID" value="XM_007377231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATU6"/>
    <dbReference type="FunCoup" id="G3ATU6">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATU6"/>
    <dbReference type="GeneID" id="18874629"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63178"/>
    <dbReference type="eggNOG" id="ENOG502S29P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068099_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATU6"/>
    <dbReference type="OMA" id="FIFELNE"/>
    <dbReference type="OrthoDB" id="5329385at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031342">
        <property type="entry name" value="Mug163-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17119">
        <property type="entry name" value="MMU163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="309" mass="34294" checksum="9267ED164D80FE5D" modified="2011-11-16" version="1">MSLIHKESLVKLVQQSSRNAQLVAKSSVTSMGQQPVITGASLASATPYKSTTMTKSPLRQNNLSKFTAVPPRHNINNKPDEINKFELEKKANLGAMIETLKAAIPNILEKSLDKSLISPDIFFRFCPSHFDEDYLPSLKGPVTYYATCKALQLFVSSIILSPKVKLHIQSIRIHTGPDAQSMFDDTTKIIVRWSTCPEGCYHLEGGEDEQSHSTSQANLGAHSWSKLDTMRLLNQNHVDSKVSLATTLTKLPATLIGLTKENKKLERVISGLFIFELTDDNSQVLVHTIEDMDIIERFAPDDVDSLTPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATV0</accession>
    <name>G3ATV0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mid2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_68441</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377297.1">
        <property type="nucleotide sequence ID" value="XM_007377235.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATV0"/>
    <dbReference type="STRING" id="619300.G3ATV0"/>
    <dbReference type="GeneID" id="18875219"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68441"/>
    <dbReference type="eggNOG" id="ENOG502SGCY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_476644_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATV0"/>
    <dbReference type="OrthoDB" id="4065319at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051009">
        <property type="entry name" value="PRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36089">
        <property type="entry name" value="CHITIN SYNTHASE 3 COMPLEX PROTEIN CSI2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36089:SF1">
        <property type="entry name" value="CHITIN SYNTHASE 3 COMPLEX PROTEIN CSI2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442564" description="Mid2 domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="332"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="550"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="86"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="561"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="572" mass="64174" checksum="66270C44F3E88B45" modified="2011-11-16" version="1">MKHFLLLIVLSTEFSSTFAASKDTPGESVTLVQPTKSAIEVLERNEAADPVIHAEFLDFQKPEGLQKQGKLVLEPFQKRATTTLFQRPTTVKQTQVRTQTQKQQLQAQAAPTPTKTQAQTTQPQRTQPQQTQPQRTTPQQTTQQQTQPQRTTPQQTQTNKPQQTQTNTQPNQNKPQDNKPEDNKPTENKPEDKKPDEEKPEDKKPEEEKPEDKKPEDDKSDDKSEEKKPEDQDGDSKDDKPEAPKVKPDEKKPDDKDDKEKDDKPAVKPNKPDNDENEADTTKPKLPDKSLPPISDLPPIQDRPKTYSYDVQIPVASKSNPYIYQSNLPTNIVFIIVGAVLGFIFVSFAGIGLIISLASRRVAKREKGFFQNDSNPSFPDSFYYGAPRFTPSYGSSDTRVNTDESSFSQGRSYRDTLFGHGAGGGAVAAHTPSICVSGVSSGAVASSEKKPGDNRRTMFVSPTLELANNKRKIPSYYKTSMERLPDNSSTASLSLKPYNSSQFNQSATFGRSSYAPDSYYGSENQMMMSETFEQPYYDYERYGTDRFYDGYDDSTGRGQRPPSQYLNNLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATX0</accession>
    <name>G3ATX0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADF-H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_63201</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the actin-binding proteins ADF family. GMF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377317.1">
        <property type="nucleotide sequence ID" value="XM_007377255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATX0"/>
    <dbReference type="FunCoup" id="G3ATX0">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATX0"/>
    <dbReference type="GeneID" id="18874638"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63201"/>
    <dbReference type="eggNOG" id="KOG1736">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087056_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATX0"/>
    <dbReference type="OMA" id="EWKMLYA"/>
    <dbReference type="OrthoDB" id="3919494at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071846">
        <property type="term" value="P:actin filament debranching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034316">
        <property type="term" value="P:negative regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11283">
        <property type="entry name" value="ADF_GMF-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011171">
        <property type="entry name" value="GMF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11249:SF2">
        <property type="entry name" value="GLIA MATURATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11249">
        <property type="entry name" value="GLIAL FACTOR NATURATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001788">
        <property type="entry name" value="GMF-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="3">
        <location>
            <begin position="2"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR001788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="137" mass="15865" checksum="256AB1F44D415E24" modified="2011-11-16" version="1">MSTALYSFSSETLAELRKFRFASARVDKMQAAIYVIDKESYEVKRDDTEIIDSVEDLIEELPDNTPRYIVLSYPFKLDDGRLKTPLVLLYWIPPTSGQESRMLYAGALEQFRDKAGVSRLIKVEDEDDFEDLQEQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU10</accession>
    <name>G3AU10_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 19</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MED19</name>
        <name evidence="11" type="ORF">SPAPADRAFT_73128</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 19 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377357.1">
        <property type="nucleotide sequence ID" value="XM_007377295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU10"/>
    <dbReference type="STRING" id="619300.G3AU10"/>
    <dbReference type="GeneID" id="18875468"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_73128"/>
    <dbReference type="eggNOG" id="ENOG502QXG3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121530_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU10"/>
    <dbReference type="OMA" id="MRGDDMS"/>
    <dbReference type="OrthoDB" id="2160599at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013942">
        <property type="entry name" value="Mediator_Med19_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28270">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28270:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08633">
        <property type="entry name" value="Rox3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="136"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="172" mass="19173" checksum="30DC842B5885F1F7" modified="2011-11-16" version="1">MTEKQINLAEGIESYYLIDSTHVYKQPKPTPIDNLLKLYGLEPIVKSLARTNPDGSKGVKLRKSYKNHIQDLPGKHQVAPAKPIPSGLLDPSAAQAPDIIKELDPELLSKAMKFEKTPINGIPGFNTADLAINDQQMLMRGDDMSENDEYGSKKSKRKKKIQNGSDPKRQHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU40</accession>
    <name>G3AU40_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Serine/threonine/tyrosine protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_63267</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377387.1">
        <property type="nucleotide sequence ID" value="XM_007377325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU40"/>
    <dbReference type="FunCoup" id="G3AU40">
        <property type="interactions" value="293"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU40"/>
    <dbReference type="GeneID" id="18874666"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63267"/>
    <dbReference type="eggNOG" id="KOG0658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_20_1"/>
    <dbReference type="InParanoid" id="G3AU40"/>
    <dbReference type="OMA" id="QHLAMEC"/>
    <dbReference type="OrthoDB" id="272141at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030437">
        <property type="term" value="P:ascospore formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF4">
        <property type="entry name" value="PROTEIN KINASE MCK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="28"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW30416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="336" mass="38840" checksum="E9EE6A6561BBB786" modified="2011-11-16" version="1" fragment="single">MPQSGIIEEYTMDRVVNNKTSSTQPMTVREYKKIGEGAFGTVVEAVLKYVDPANNKSHDDDKNNSEWLGPFAIKRVPAQTEYKSRELEILRLVDHPNIVSLRFFFDKARPDTREVYQNLVMECLPSNLQAEIKYYRQSKYTIPYPHMKAYTFQLTRAMLYLHGFGISHRDIKPSNILVDPHTVQLKICDFGSAKKLEPNQPSVSYICSRYYRSPELIVGCTLYTTKIDIWGLGCVVAEMFLGKPIFQGQSPETQLREIAKLLGPPPNTFFFKSNPHYRGNMYTTRLFSCTVEERFKQIFSNSPPDAIDLLLKILVYDPEFRPSPRRVLVHPFFNEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU62</accession>
    <name>G3AU62_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">60S ribosomal protein L23</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_63277</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377409.1">
        <property type="nucleotide sequence ID" value="XM_007377347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU62"/>
    <dbReference type="FunCoup" id="G3AU62">
        <property type="interactions" value="1133"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU62"/>
    <dbReference type="GeneID" id="18874671"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63277"/>
    <dbReference type="eggNOG" id="KOG0901">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095071_3_0_1"/>
    <dbReference type="InParanoid" id="G3AU62"/>
    <dbReference type="OMA" id="IRQSKPW"/>
    <dbReference type="OrthoDB" id="407959at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000003">
        <property type="entry name" value="60S ribosomal protein L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_uL14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L14/L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="137" mass="14488" checksum="EC11E72880A4787F" modified="2011-11-16" version="1">MSGSGASGNKFRMSLALPVGAIMNCADNSGARNLYVLAVKGIGARLNRLPAASAGDMVMATVKKGKPELRKKVMPAIVIRQSKPWRRKDGVYLYFEDNAGVIVNPKGEMKGSAITGPVAKECADLWPRIASNSGVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUD8</accession>
    <name>G3AUD8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Complex I-B22</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">NADH-ubiquinone oxidoreductase B22 subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_52592</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Mammalian complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I LYR family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377485.1">
        <property type="nucleotide sequence ID" value="XM_007377423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUD8"/>
    <dbReference type="STRING" id="619300.G3AUD8"/>
    <dbReference type="GeneID" id="18871698"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52592"/>
    <dbReference type="eggNOG" id="ENOG502S7CC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108081_1_1_1"/>
    <dbReference type="InParanoid" id="G3AUD8"/>
    <dbReference type="OMA" id="KWERNAP"/>
    <dbReference type="OrthoDB" id="13598at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20263">
        <property type="entry name" value="Complex1_LYR_NDUFB9_LYRM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045292">
        <property type="entry name" value="Complex1_LYR_NDUFB9_LYRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033034">
        <property type="entry name" value="NDUFB9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12868:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12868">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B22 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="17">
        <location>
            <begin position="15"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="75"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW30514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="107" mass="12430" checksum="AE6B8BE4C41FE1C4" modified="2011-11-16" version="1">MSSPILPFTEANTKRVSALLRRSLRLAWDHTTKFQAYRTATIQIRQQFESNRDVNDPQQLQDIIEKTEAKLYEWRNPDPVIPPSRPGGTKYERNLKLPAEPVVPGDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUH1</accession>
    <name>G3AUH1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone chaperone RTT106</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Histone chaperone rtt106</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_63102</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RTT106 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377228.1">
        <property type="nucleotide sequence ID" value="XM_007377166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUH1"/>
    <dbReference type="STRING" id="619300.G3AUH1"/>
    <dbReference type="GeneID" id="18874595"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63102"/>
    <dbReference type="eggNOG" id="ENOG502R9PE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1071781_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUH1"/>
    <dbReference type="OMA" id="LIMVECH"/>
    <dbReference type="OrthoDB" id="75754at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13304">
        <property type="entry name" value="PH2-like_Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050454">
        <property type="entry name" value="RTT106/SSRP1_HistChap/FACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040770">
        <property type="entry name" value="Rtt106_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849:SF3">
        <property type="entry name" value="HISTONE CHAPERONE RTT106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18469">
        <property type="entry name" value="PH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="13">
        <location>
            <begin position="77"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="173"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="173"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW30257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="256" mass="29294" checksum="D28C09E6D592BE3F" modified="2011-11-16" version="1">MIKQGKLPADIESQFTFDKNALNLNPIQERIIDYFKRQFKLCGIDMINYLPCDFFNNKFTLNHDNAIALSVTNTPSLIMVECHKGAKDGVLLLNSSYLIFGFKKPILIFQISQIKQASYTNITRVTFSVMFIVVTERNEERTLEFSMIDQQFFQVIDDFIKLNNIKDDSYNEELKEKTDTKSDTAQAGDVATTTSTENNNVINVDDDDSEEDADFQDEDESEVDEEFDSDAGANSDNNEDEDEVFNKGKEEEEDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUI5</accession>
    <name>G3AUI5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA/RNA-binding protein Alba-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_63383</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377512.1">
        <property type="nucleotide sequence ID" value="XM_007377450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUI5"/>
    <dbReference type="GeneID" id="18874722"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63383"/>
    <dbReference type="eggNOG" id="ENOG502T5RX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1594303_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUI5"/>
    <dbReference type="OMA" id="IAKLICI"/>
    <dbReference type="OrthoDB" id="4020196at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="2">
        <location>
            <begin position="30"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="164" mass="18466" checksum="74248B47AE458889" modified="2011-11-16" version="1">MEALQEQFTKLVNETTHSISRSLRGTSPSIIKITKNDSIKQKVTSITAQLKENKIVLIASLSNSIPKAISVIEIAKSALKEENKSVKQFNKLAHLDSTHNPNYKPNQENLEGSKHANDDEDEHKQVIERDILESINGPKVYQLPIMYVILSLDNMISLTDWTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUS9</accession>
    <name>G3AUS9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flo11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_52704</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377606.1">
        <property type="nucleotide sequence ID" value="XM_007377544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUS9"/>
    <dbReference type="GeneID" id="18871710"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52704"/>
    <dbReference type="HOGENOM" id="CLU_771996_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUS9"/>
    <dbReference type="OrthoDB" id="4028176at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018789">
        <property type="entry name" value="Flo11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51824">
        <property type="entry name" value="FLO11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flo11" evidence="2">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Flo11" evidence="2">
        <location>
            <begin position="176"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="335"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="359" mass="40619" checksum="2B27B2EAD427A19F" modified="2011-11-16" version="1">MCNYPFGETSSQVSGTPKLEIFEYKSIKPIAGSDNLYEVTLEFKVEDTDPSVQRSIIFQNPQIPSWVSSMNMRFDYNGNAANHFIIKWPMVIQHGDGNQLCTTPFSIELLMIRDIWNPDYYYYYHDCATGVSQGDYPLQCWSECTEQSQPEPTTATSYIDQPITNVATDINPLPTPINCPLEWNGDSVCDVPIGSYASSTGAPPVEIFRFKSIRYIKDGLYEWVFEFKINPTGSEGDLNMAYVQNFISHNNGATPQPQIIQVNDFDYSGGSPFHFYLLWQQPSQTIGDLTCTTPFTILYYFTNGMYTYKHGCDADGTDLPLQCWKEICPPEESATITEESATSSAKTTTKSHCKRKVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV18</accession>
    <name>G3AV18_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">tRNA(His) guanylyltransferase</fullName>
            <ecNumber evidence="3 14">2.7.7.79</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 14">tRNA-histidine guanylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">SPAPADRAFT_144742</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 14">Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a 5'-end ribonucleotide-tRNA(His) + GTP + ATP + H2O = a 5'-end phospho-guanosine-ribonucleotide-tRNA(His) + AMP + 2 diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54564"/>
            <dbReference type="Rhea" id="RHEA-COMP:14193"/>
            <dbReference type="Rhea" id="RHEA-COMP:14917"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:141847"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.7.79"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="16">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the tRNA(His) guanylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.79" evidence="3 14"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377858.1">
        <property type="nucleotide sequence ID" value="XM_007377796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV18"/>
    <dbReference type="FunCoup" id="G3AV18">
        <property type="interactions" value="761"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV18"/>
    <dbReference type="GeneID" id="18870660"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144742"/>
    <dbReference type="eggNOG" id="KOG2721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044271_1_0_1"/>
    <dbReference type="InParanoid" id="G3AV18"/>
    <dbReference type="OMA" id="WKQHTEI"/>
    <dbReference type="OrthoDB" id="62560at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008193">
        <property type="term" value="F:tRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3000:FF:000001">
        <property type="entry name" value="tRNA(His) guanylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025845">
        <property type="entry name" value="Thg1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024956">
        <property type="entry name" value="tRNAHis_GuaTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007537">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038469">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12729">
        <property type="entry name" value="TRNA(HIS) GUANYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12729:SF6">
        <property type="entry name" value="TRNA(HIS) GUANYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04446">
        <property type="entry name" value="Thg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14413">
        <property type="entry name" value="Thg1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028980">
        <property type="entry name" value="tRNAHis_guanylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14 15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10 14 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 14 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNAHis guanylyltransferase catalytic" evidence="18">
        <location>
            <begin position="6"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Thg1 C-terminal" evidence="19">
        <location>
            <begin position="141"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="233"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="29"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="75"/>
            <end position="76"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR028980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR028980-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR028980-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF14413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EGW30092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="267" mass="32062" checksum="1F3124772BE9C2D1" modified="2011-11-16" version="1">MANSRYEYVKSFERENYLLPDTYVVIRVDGKGFHKFSQHYQFTKPNDIAALQVMNRAAHKIMTKYSDVMMAYGDSDEYSFLLRRQCELYERREMKLCTLFASLMSSYYSFYWNQAFPDKQLEEDHLPVFDGRAVVYPNFNVVRDYFSWRQVDCHINNLYNTTFWNLVIKQELTPQQAEHKLMGTVSSDKNEILFQMGINYNNEPEMFKKGTIYVRELKDYNTKDESNLSSRQKSREDKKRKKADIVEHHVDIINDDAWWDSRPWLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV70</accession>
    <name>G3AV70_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2415 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63492</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377639.1">
        <property type="nucleotide sequence ID" value="XM_007377577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV70"/>
    <dbReference type="FunCoup" id="G3AV70">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="GeneID" id="18874779"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63492"/>
    <dbReference type="eggNOG" id="KOG4532">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027079_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV70"/>
    <dbReference type="OMA" id="RPGHQSG"/>
    <dbReference type="OrthoDB" id="418169at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019417">
        <property type="entry name" value="DUF2415"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991:SF9">
        <property type="entry name" value="DUF2415 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10313">
        <property type="entry name" value="DUF2415"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2415" evidence="1">
        <location>
            <begin position="316"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW29873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="614" mass="69892" checksum="7C8EDF3F0834F2A9" modified="2011-11-16" version="1">MTIDVSHPNKLKTINNFSNSSVSKKKSKFINSSQQYNKCFQNYYHSYEYLKPNPIYIPHIRPSVNHWQLRDLLKFNEVEDLVYYTRDDSIYSYDLNKETTKNYIKLNYYPRCFSHHDDIIVTGGLLTSSSKLFSLNLDNLTSTNTNIPARRIAKGLFSFYSPKTHTSKTVKLGEMINNDVTIYPSSTNSYQSYVCNNDTHLYGLDINNNGNIKVTCKINCEINTCLNNVVQNPRTDKILTATGDSSSIFLIDPHQPKPIIKTIKSGHESGFGVSYHPNGYLFSTVFQDGTCQIYDIRNLKSRLIDIKSTRPGHQSGAFRVCKFSPANDMNDLLIISEHVGRVHLIDLRNLDYDNVDDHQVIVVPVALDQFGDYQATIKNPHLIKRREERALKKKSQDVMHKEDITEPDEADVKPRQKFPVAVYPQTSDSFTAPIVYDYDYLTNVSPRLFKEFSYIPPPPPPAPMQYNPPPKLNYPQWNESGPIELEGTSIEHVSPTSSARPSISQTIEDFDTNPDVAAHLESVYNSMDWASAPPGIAPSMSTRSDSYIYSNYCDDSYQQCTNHIHGEMELSGIEFCDPHSTNDSKIMIGCQDAGILMWDINGVSRRSFGRFDYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV71</accession>
    <name>G3AV71_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_63493</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377640.1">
        <property type="nucleotide sequence ID" value="XM_007377578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV71"/>
    <dbReference type="FunCoup" id="G3AV71">
        <property type="interactions" value="1152"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV71"/>
    <dbReference type="GeneID" id="18874780"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63493"/>
    <dbReference type="eggNOG" id="ENOG502RQBX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1448567_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV71"/>
    <dbReference type="OMA" id="TIKEPHA"/>
    <dbReference type="OrthoDB" id="1431247at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00298">
        <property type="entry name" value="ACD_sHsps_p23-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="60"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW29874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="187" mass="21674" checksum="E209086921471E4B" modified="2011-11-16" version="1">MLRRTAANIPKALIPRVRGYRTWYDPRLPLPLVPRYKDSFFRLPEIFRDPLARDFDINWFDNKSMNDYFQTTEEKDKYVVNIKDANLADKNVKLDFNKNENSLKISIEQKMDQEDKGNTSSFASSFSSNYQFEKPVSVDSIVAEHGKDGLTISLPKLEVEKDDDDDVVNIKINRGENESESTPTENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV83</accession>
    <name>G3AV83_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="13">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_63507</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 13">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12 13">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 13">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 13">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="13"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377652.1">
        <property type="nucleotide sequence ID" value="XM_007377590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV83"/>
    <dbReference type="FunCoup" id="G3AV83">
        <property type="interactions" value="454"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV83"/>
    <dbReference type="GeneID" id="18874786"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63507"/>
    <dbReference type="eggNOG" id="KOG2467">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022477_3_0_1"/>
    <dbReference type="InParanoid" id="G3AV83"/>
    <dbReference type="OMA" id="TQPFFSQ"/>
    <dbReference type="OrthoDB" id="10265628at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF57">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 13" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9 12 13" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="14">
        <location>
            <begin position="1"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW29886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="381" mass="41704" checksum="2F1E51269C15B38F" modified="2011-11-16" version="1" fragment="single">ALEAFGLDPAEWGVNVQPLSGAPANLYAYSAILEVGDRIMGLDLPHGGHLSHGYQTATTKISYISKYFQTMPYRLNEETGLIDYDMLEKTATLFRPKVIVAGASAYSRVIDYARMKKIATKVGAYLMSDMAHTSGLVSAGVTDSPFPHSDIVTTTTHKSLRGPRGAMIFFRKGIRKVTKKGKEIPYELEQKINFSVFPGHQGGPHNHTISALAVALKQCQEPEYKQYQQDVVDNAKHFADTLLERGFKLVSGGTDTHLILIDLRSKNIDGARVEAVLERANIAANKNTVPGDVSALFPSGLRVGTPAMTTRGFGKEDFTKVSEYIDRAVEISKELKQQEQGKVPKELLASFKKLADESSAVKELGEEVAKWASTFPVPGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV87</accession>
    <name>G3AV87_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP-dependent RNA helicase DOB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_57398</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377656.1">
        <property type="nucleotide sequence ID" value="XM_007377594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV87"/>
    <dbReference type="FunCoup" id="G3AV87">
        <property type="interactions" value="1210"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV87"/>
    <dbReference type="GeneID" id="18872116"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57398"/>
    <dbReference type="eggNOG" id="KOG0948">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002902_0_1_1"/>
    <dbReference type="InParanoid" id="G3AV87"/>
    <dbReference type="OMA" id="IMLKNYN"/>
    <dbReference type="OrthoDB" id="64767at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034458">
        <property type="term" value="F:3'-5' RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004532">
        <property type="term" value="F:RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071031">
        <property type="term" value="P:nuclear mRNA surveillance of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071042">
        <property type="term" value="P:nuclear polyadenylation-dependent mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000292">
        <property type="term" value="P:RNA fragment catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140746">
        <property type="term" value="P:siRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18024">
        <property type="entry name" value="DEXHc_Mtr4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13154">
        <property type="entry name" value="KOW_Mtr4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000083">
        <property type="entry name" value="ATP-dependent RNA helicase DOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000141">
        <property type="entry name" value="ATP-dependent RNA helicase DOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.300:FF:000001">
        <property type="entry name" value="Mtr4 exosome RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.30:FF:000002">
        <property type="entry name" value="superkiller viralicidic activity 2-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025696">
        <property type="entry name" value="Beta-barrel_MTR4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048392">
        <property type="entry name" value="MTR4-like_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016438">
        <property type="entry name" value="SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012961">
        <property type="entry name" value="Ski2/MTR4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF7">
        <property type="entry name" value="EXOSOME RNA HELICASE MTR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08148">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21408">
        <property type="entry name" value="MTR4-like_stalk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13234">
        <property type="entry name" value="MTR4_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005198">
        <property type="entry name" value="Antiviral_helicase_SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01142">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="131"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="368"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="18"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="332"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW29890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1043" mass="117973" checksum="C6CAD18DF83B2FC6" modified="2011-11-16" version="1">MDDLFDVFDEAPAAAPAKIEEIKHVEHKKRQSENTAKQTGPKKQKTEAKPEIKPVVFDSVEIEASREVAVSAGLLSSATPTPEDKLKLRHQVRHQVAIPPSYPYTPIAEHKRVKEARTYPFVLDPFQDTSISCIDRNESVLVSAHTSAGKTVVAEYAIAQSLRDKQRVIYTSPIKALSNQKYRELQAEFGDVGLMTGDVTINPDAGCLVMTTEILRSMLYRGSEVMREVAWVIFDEVHYMRDKSRGVVWEETIILLPDKVHYVFLSATIPNAMEFAEWIVKIHNQPCHVVYTDFRPTPLQHYLFPAGGDGIHLVVDEKGTFREENFQKAMATISDNTGDDPSSADKGKGKKGQTFKGGKGDASSDIYKIVKMIYMKRYNPVIVFSFSKRDCEKLALKMSKLDFNTDEEREALTKIFQNAINLLPEADKDLPQIKNILPLLKRGIGIHHSGLLPILKEIIEILFQEGFLKVLFATETFSIGLNMPAKTVVFTSVRKWDGVGFRWVSGGEYIQMSGRAGRRGLDDRGIVIMMIDEKMEPQVAKGMVKGQADRLDSAFHLGYNMILNLMRVEGISPEFMLESSFFQFQNAASVPVMQKSMDELSVELEQIQVDDEPTVKDYYEFKAQLTKYQADMRKVITHPGHILPFLQEGRVVKVKVGEHDYDWGMVVSFAKRNAKNQDFTPHEAYIVTVFVCTMFVDSPVNLIKSFNPVLPEGIRPAVRGSEKSRAEYIPITLDSIEKISSVRLRVPSDLKSAEAKRSLVKTLKDLPKRLPDGIPLMDPVESMKINDNEFKLLLRKIDVLEAKLVSNPLHNTARLAPLYEKYASKVEIENKIKSLKEKILEAEAVIQLDDLRNRKRVLRRLGFTKTDNDIIELKGRVACEISTGDELLLTELIFNGNFNELTPEQCAALLSCFVFQERAKEVPRLKPELAEPLKSMQEMATKVAKVSRECKIDIIEKDYVESFRAELMEVTFAWCKGATFSQICKMTDVFEGSLIRMFRRLEELIRQLVIAAKAIGNSELETKMEAALELVHRDIVSAGSLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVK3</accession>
    <name>G3AVK3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_143982</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377718.1">
        <property type="nucleotide sequence ID" value="XM_007377656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVK3"/>
    <dbReference type="STRING" id="619300.G3AVK3"/>
    <dbReference type="GeneID" id="18870588"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143982"/>
    <dbReference type="eggNOG" id="ENOG502S5BH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030508_0_2_1"/>
    <dbReference type="InParanoid" id="G3AVK3"/>
    <dbReference type="OrthoDB" id="276515at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09083">
        <property type="entry name" value="EEP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF36">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442730" description="Endonuclease/exonuclease/phosphatase domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="76"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW29952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="301" mass="33353" checksum="A1703A653994A31B" modified="2011-11-16" version="1">MNSLSISFWPLYSLLSLVATAISVPDTEVSTSAIFQVYTNNIRYDAKDLMSMELPWAERKVGVVGTMLAVSANMSTVFALQEVTYNQLLDIKTEMNWNSQSWAHIGEGRDGGVAGEYNPILYNVNEWDLLSNYTRWLSETPGTPSAYKGKCFNRIVSIGIFRHKSTGSIVNVLNTHFCHKYEDSRQFQAYEILKHVDAIGLGNPTIVAGDLNSERTSVAYSILSDVVKEHGDPLELPTVSGFQSGGKGSTIDYVFSKGNLVSLSHAVLDNMLYDQYRFSDHRPVLEVFELAPTLVARACAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVT2</accession>
    <name>G3AVT2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphoinositide 5-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_52826</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptojanin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the central section; belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.36" evidence="3"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377743.1">
        <property type="nucleotide sequence ID" value="XM_007377681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVT2"/>
    <dbReference type="FunCoup" id="G3AVT2">
        <property type="interactions" value="388"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVT2"/>
    <dbReference type="GeneID" id="18871723"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52826"/>
    <dbReference type="eggNOG" id="KOG0566">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003016_2_0_1"/>
    <dbReference type="InParanoid" id="G3AVT2"/>
    <dbReference type="OMA" id="HPCHELR"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043813">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF257">
        <property type="entry name" value="PHOSPHOINOSITIDE 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC" evidence="6">
        <location>
            <begin position="143"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="919"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="954"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="981"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1019"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1042"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1088"/>
            <end position="1098"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW29977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1134" mass="126414" checksum="2CC190FB61099B44" modified="2011-11-16" version="1">MRLLYKEEPRTIAIQSHTHTLRFRAQPSSGETKKPLVNLELVPNSHITKHQGYRALLSKEIYGCLGLAYVDNQTYVAIITGAIVNVANPLGNETVDRIYSIDFISLEDNYWDHKLLDSSGFPVISAEDPDDDGVRIQHPCIDLKKLVSNGTFYYSNDFDLTSLLQNRGVNTEQGARSIDHYSKEYMWNSFLMEEMIKYRTNLDHQAQQILDENKFLTVAIRGFAKTVQLGANDSITLISKQSWKRAGTRYNTRGIDDYGNVANYVETEFIYNNPSNSSIFSFVQIRGSVPTFWEQDSTLINPKITLTRSSEATQPIFNAHFKQICENYGVTHIVDLLSKTKPAESQLSNRYKQLYEQCDRNNEIEYTAFDFHQETKVGGFAQAGKIIPLLHKSIEQFGWFYYDLNAEEIITRQDGIFRVNCLDCLDRTNLIQQIICRNILQNILSNTGSRRSEESLMLRYNSLWADNGDAISQIYTGTNALKSSFSRSGKMNIAGALSDVTKSVSRMYQNTFVDSKKQSTIDLLLGVDGKYSKKVKIFDPLHEFVNGKLKQQAGTFTSFKNIKCFTGTYNVNALEPESHIDLSSWLFPPASSSADIYGIGFQELIDLDPRSILNADTTKAGKWAEILNRELNKHGEQYVLLRVESIASMSLFLFVKKSQAVHVTKVSGSSKKTGLGGMTANKGACAVRFNFGDTSFVMITCHLAAGMQATLERYNDYSTILQGLLFPRNYSIRDHDHIIWFGDLNYRINMMNEQCRDSVARGEYNELIQMDQLNEERRHDGAFKPFNEGVISFAPTYKFDKGTSNYDSSEKQRVPSWTDRVLFTSSTGLSKDLTLLKYDSVMDMVLSDHKPVYAVFDVKVNFIDREMKGKIAKELYNVYKREHGGELVSLSSNSSVSSSAGTVDFSTDTLSELNLLDDDINEGAPKLPQRPARRIPPPPTSRKVAPPMQELKPAPVPRRLPPTPNSAPPPPLPRRVPPPSSVESSPVIPASASASVSPTISPKLQNVPIGFSSAPLIPTRSNTAGSLSPNITPVKSAASLPAKSTSSIPVKSTTSLPTKTATPPPQAFKPLVPSKPSALASRKLSIGKSEDEKDEKVNNSDSDTGSDARLPPPAPRQANSVGMSMSDWKPLIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVV4</accession>
    <name>G3AVV4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_144412</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377765.1">
        <property type="nucleotide sequence ID" value="XM_007377703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVV4"/>
    <dbReference type="STRING" id="619300.G3AVV4"/>
    <dbReference type="GeneID" id="18870624"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144412"/>
    <dbReference type="eggNOG" id="KOG0519">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_3_2_1"/>
    <dbReference type="InParanoid" id="G3AVV4"/>
    <dbReference type="OMA" id="QLIHLMG"/>
    <dbReference type="OrthoDB" id="10266508at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097308">
        <property type="term" value="P:cellular response to farnesol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036180">
        <property type="term" value="P:filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900445">
        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06225">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000002">
        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1530:FF:000001">
        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1530:FF:000002">
        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000015">
        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1530">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18947">
        <property type="entry name" value="HAMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158472">
        <property type="entry name" value="HAMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="HAMP" evidence="15">
        <location>
            <begin position="72"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="15">
        <location>
            <begin position="166"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="15">
        <location>
            <begin position="258"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="15">
        <location>
            <begin position="350"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="15">
        <location>
            <begin position="442"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="13">
        <location>
            <begin position="516"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="14">
        <location>
            <begin position="885"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1041"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1061"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1140"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="11">
        <location>
            <position position="934"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW29999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1152" mass="125858" checksum="F8B944BDC4616E65" modified="2011-11-16" version="1">MTGTNEIKIQNGRNANVDIGSSAEILKLLEDPNALQDSSLDLRDVVIKHIRKQDKLIDGLSHELDTSKTANKAFKQALSEIGTVVISVAMGDLSKKVEIHSVESDPEILKVKITINTMMDQLQTFANEVTKVATEVANGELGGQAKNEGSVGIWRSLTDNVNIMALNLTNQVREIADVTRAVASGDLSRKINVHAQGEILQLQMTINTMVDQLRTFAFEVSKVARDVGVLGILGGQALIENVEGIWKELTDNVNAMALNLTTQVRNIANVTTAVAKGDLSKKVTADCKGEILNLKLTINQMVDRLQNFALEVTTLSREVGTLGILGGQANVQDVEGAWKAVTENVNLMATNLTNQVRSIATVTTAVAHGDLSQKIDVHAQGEILQLKNTINKMVDSLQLFAFEVSKVARDVGINGKLGIQAQVSDVDGLWKEITSNVNTMASNLTSQVRAFAQITAAATDGDFTRFITVEASGEMDALKTKINQMVLNLRESIQRNTAAREAAELANSAKSEFLANMSHEIRTPLNGIIGMTQLSLDTELTQYQREMLSIVHNLANSLLTIIDDILDISKIEANRMTVEQIDFSLRGTVFGALKTLAVKAIEKSLDLTYQCDSSFPDNLIGDSFRLRQVILNLAGNAIKFTKQGKVSVSVKKSDKYIEGSDKLLLEFCVRDTGIGIEKDKLGLIFDTFCQADGSTTRKFGGTGLGLSISKQLIHLMGGEIWVTSEYGTGSNFYFTVSVSPSNIRYTRQTEQLLPFSGHYILFVSAEHTDSELEEIRANVIELGLNPVIVRDISDANLTDPVKYDIIMIDSLETAKKLRLLSEVKYIPLVLVHHSIPELNMRVCIDLGISSYGNTPCSITDLASAIIPALESRSIAQNSDESVVYKILLAEDNLVNQKLAVRILEKQGHQVEVVENGLEAFEAIQKNKYDVVLMDVQMPVMGGFEATEKIRQWEKKSNPIDSLSFRTPIIALTAHAMLGDREKSLAKGMDDYVSKPLKPKLLMQTIHKCIHNINQLKELSRQNNSNRTSNFAKNLQKSAIKSGHSEAGGAVDDGRDTLGCQDDNNATFGSGSMSSPLTGTSIPSTRSAPQSSDINSQRTKKANSMPFINISGVSRPGQLRAETRTRSDTSDTFTSAKISEVLDDDMDVDTPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE89</accession>
    <name>G3AE89_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58837</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373035.1">
        <property type="nucleotide sequence ID" value="XM_007372973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE89"/>
    <dbReference type="GeneID" id="18872636"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58837"/>
    <dbReference type="HOGENOM" id="CLU_2661402_0_0_1"/>
    <dbReference type="InParanoid" id="G3AE89"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="76" mass="8763" checksum="10F4019CC7ACFAD6" modified="2011-11-16" version="1" fragment="single">MSPKIRVRGPRLRSQKVHVTKETDIIDNDTVERYLVQIHLSSEVLSKLSYPFIETARNENNATPEIQVENTREASI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEK1</accession>
    <name>G3AEK1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_57831</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL6 family.</text>
    </comment>
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        <property type="protein sequence ID" value="EGW34763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007372113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEK1"/>
    <dbReference type="FunCoup" id="G3AEK1">
        <property type="interactions" value="1220"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEK1"/>
    <dbReference type="GeneID" id="18872211"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57831"/>
    <dbReference type="eggNOG" id="KOG1694">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066767_2_1_1"/>
    <dbReference type="InParanoid" id="G3AEK1"/>
    <dbReference type="OMA" id="KWYNADD"/>
    <dbReference type="OrthoDB" id="2436667at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13156">
        <property type="entry name" value="KOW_RPL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000014">
        <property type="entry name" value="60S ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000915">
        <property type="entry name" value="60S_ribosomal_eL6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049633">
        <property type="entry name" value="Ribosomal_eL6_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041997">
        <property type="entry name" value="Ribosomal_eL6_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10715">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10715:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01159">
        <property type="entry name" value="Ribosomal_L6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01170">
        <property type="entry name" value="RIBOSOMAL_L6E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="177" mass="19796" checksum="707D2D40971AE7ED" modified="2011-11-16" version="1">MSQIAPKWYQSEDVAAPKKARKTARPQKLRASLVPGTVLILLAGRFRGKRVVYLKNLEDNTLLVSGPFKVNGVPLRRVNARYVIATSTKVNVEGVDVSKFNVEYFAREKVTRAAKKSEADLFNEVPAKKEIKEERVADQKTVDAALLAEIKKTPLLKQYLSASFSLKSGDKPHLLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF50</accession>
    <name>G3AF50_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_57934</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372251.1">
        <property type="nucleotide sequence ID" value="XM_007372189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF50"/>
    <dbReference type="GeneID" id="18872255"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57934"/>
    <dbReference type="HOGENOM" id="CLU_680726_0_0_1"/>
    <dbReference type="InParanoid" id="G3AF50"/>
    <dbReference type="OrthoDB" id="10615629at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="405" mass="46728" checksum="DB4647D46DA098AB" modified="2011-11-16" version="1" fragment="single">MPKRRVVSQPLLSNVKELPDVPNVEPLEEEEEEDLEQMHSNSSKSSSTSVHDSRHEIASQESNIRPPPPSYNNSYNSQYLHSKHSSTYSSNSIEYMLNYPSQKHQEPPQSPDSSYNSVNSLYYTPQEYHASYALDSIEYPKLETSQYRTKQQSDSKNTSYGTYKLHFRPTEYRSSFSDDNMDYLSHEFSNLEIAKSNEDSLFSNPLVDPGSSQEDLYLTISPEETKKQLSPVPEILPKVRKSNSKHKPKPPVFHNSLYSEKHTIIEQVKQRPFSYCLDSKHNYEDSRYPIAPPIKKVQDINKPRATKRNSTLTINTTNTNVNEQRRSVSDLQQYIKTSKEKFNNNRIVSFVREKSKEVTPPKQEMPKVEKKLSFARENTKESVVTSKKEKPRTEKTLPAPPTVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF77</accession>
    <name>G3AF77_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Phosphomannomutase</fullName>
            <ecNumber evidence="5 13">5.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_57959</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 13">
            <text>alpha-D-mannose 1-phosphate = D-mannose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11140"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="EC" id="5.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the eukaryotic PMM family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.8" evidence="5 13"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372278.1">
        <property type="nucleotide sequence ID" value="XM_007372216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF77"/>
    <dbReference type="FunCoup" id="G3AF77">
        <property type="interactions" value="734"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF77"/>
    <dbReference type="GeneID" id="18872268"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57959"/>
    <dbReference type="eggNOG" id="KOG3189">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065642_0_1_1"/>
    <dbReference type="InParanoid" id="G3AF77"/>
    <dbReference type="OMA" id="ISHRVYT"/>
    <dbReference type="OrthoDB" id="10264771at2759"/>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00424"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180047">
        <property type="term" value="P:dolichol phosphate mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02585">
        <property type="entry name" value="HAD_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1240.20:FF:000001">
        <property type="entry name" value="Phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005002">
        <property type="entry name" value="PMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043169">
        <property type="entry name" value="PMM_cap"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466:SF0">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03332">
        <property type="entry name" value="PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00445">
        <property type="entry name" value="alpha-phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01143">
        <property type="entry name" value="C2.B.3:_Phosphomannomutase_Lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01140">
        <property type="entry name" value="C2.B:_Phosphomannomutase_and_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="251" mass="28857" checksum="1B8399027512E6C3" modified="2011-11-16" version="1">MSFTNKENPKVLVLFDVDGTLTPARLFISDEMKETLEKLRKKVVIGFVGGSDFSKQVEQLGPNILEEFDYCFSENGLTAYKLGKQLASQSFINWIGEEKYNKLAKFILKYLSEIDIPIRRGTFIEFRNGMINVSPIGRNASTQERHDYEAFDKVHNIREKMVAALRENFADYGLTYSIGGQISFDVFPTGWDKTYCLQHVADEHFDEIHFFGDKTYKGGNDYEIYNDPRTIGHSVTNPNDTIKILNELFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFE1</accession>
    <name>G3AFE1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_145586</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372342.1">
        <property type="nucleotide sequence ID" value="XM_007372280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFE1"/>
    <dbReference type="FunCoup" id="G3AFE1">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFE1"/>
    <dbReference type="GeneID" id="18870691"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_145586"/>
    <dbReference type="eggNOG" id="ENOG502QU87">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001720_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFE1"/>
    <dbReference type="OMA" id="TKVEWLW"/>
    <dbReference type="OrthoDB" id="10070851at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd07609">
        <property type="entry name" value="BAR_SIP3_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13280">
        <property type="entry name" value="PH_SIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039463">
        <property type="entry name" value="Sip3/Lam1_BAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042067">
        <property type="entry name" value="Sip3_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14248">
        <property type="entry name" value="CYCLIN Y, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1000"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="323"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="8">
        <location>
            <begin position="761"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1189" mass="137289" checksum="D8E917AE06505B71" modified="2011-11-16" version="1">MNSTSRAQQRSNSSSSREQHSARSPSSDDTAQQPVLTRQFKLISVNFKEAALDSPSFRASVNHLDIQVDNIEKWLIAISSSVKKIPRYVNDLQSFCNSFLEHLTPTFLQEGLIDQEYTVPALHTTLAGLKKIWGLSFQALNVNLHALEEIDSFIYSEVPRYKELRKKFDEKQAKYDKYLSVYVSIPKAKETPMVMDDARELFLVRKEYIHVSLDLIIQLQNLGNKLNKLFVNMNAELFRSKWNVFSPLNSDSTFRDQWEKVQKIQHWNDSYATAVDKLTPDMLSAREQVEEGCHIQFQPSLNADDYRTFSINAKSLDDIDEQGIEKHGYLFMKTWTEKSSKPIWVRRWAFIKGGIFGLLVLSPSHTFVQETDKLGVLLCNVRYNPNEDRRFCFEVRTSDITLVFQAESLIELKSWIKVFQNEKNRVLGTDEEEGLVKIASGRYPPIILELASTLSTVMDREFTSTRIVNAEGQIITSSTLSNHIEKNEKYFQKYIYFQIPRIRPPFITDTTKSSIIAYSLASVTSVPTALTANIWGSVNWGIYYLHDLVQDGPPISTYGIEPDHEMIEYQEDNLGNGIYHPSYYPKELVSLDIQMRSLFESAIEPGEYCILSFSCIWSPNSMQELGGRCFITTHRMYFYMQASGFIALFKGFVGSLVSVDYTSQKQYDLLKIYNVNGIIKMKLFLDDGKLIKKKLVYIINNVASDKPKPLREVLAELAEIEVKLSKGLPSSEKRLILGDASSLVPAIEGKQSVHKVDFSDEYDLVGDSTFDLPPKAIFHALLGDNSVIFTDETVFTVKEYYIKKPWRTSSRGTLYRKLNVPAKYGTNLGEVHIRQEIDNMEDNSYYTFTYARSKFTFFYGPRFSAIYKIVIVGIAGKRSRVMFYCKAVFDRSTFYNWMLKRFCVRVDMSQIKRIQKRLKQAEKEVGKHGMIVKSIYLYGKLSHTDEPEEELPDGLVKFGFGFVARFMLIRMTNKAVTTTYNTIMQILRLLIIFLKSVRMNQFLLFIIFGSTLLNLYLFGKTTTSYWTAKRANQLAREYVTKDPIVLQRSVYLKDIQEHVNNRNISFGNPKVFGLFKNISFVHSFNYEPHWTDDYFDDNTRDVAKQLRLSFQEIGVKRHELLVKLNILNDMEREIAQAEWNNWLMSEVQRCDYLKENILDSLKIDENVVKGIETVMGFCEECREALAGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFM3</accession>
    <name>G3AFM3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Histone H3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_58138</name>
        <name evidence="14" type="ORF">SPAPADRAFT_59415</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907</strain>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372424.1">
        <property type="nucleotide sequence ID" value="XM_007372362.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373587.1">
        <property type="nucleotide sequence ID" value="XM_007373525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFM3"/>
    <dbReference type="SMR" id="G3AFM3"/>
    <dbReference type="FunCoup" id="G3AFM3">
        <property type="interactions" value="934"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFM3"/>
    <dbReference type="GeneID" id="18872338"/>
    <dbReference type="GeneID" id="18872905"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58138"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59415"/>
    <dbReference type="eggNOG" id="KOG1745">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078295_4_0_1"/>
    <dbReference type="OMA" id="HIFAEMA"/>
    <dbReference type="OrthoDB" id="5326060at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000010">
        <property type="entry name" value="Histone H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00322">
        <property type="entry name" value="HISTONE_H3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="10 11" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="13">
        <location>
            <begin position="44"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW35012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="136" mass="15388" checksum="A613633B480AC67A" modified="2011-11-16" version="1">MARTKQTARKSTGGKAPRKQLASKAARKSAPSTGGVKKPHRYKPGTVALREIRRFQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAIGALQESVEAYLVSLFEDTNLCAIHAKRVTIQKKDIQLARRLRGERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFS0</accession>
    <name>G3AFS0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Nuclear cap-binding protein subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">20 kDa nuclear cap-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_131749</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the RRM NCBP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372471.1">
        <property type="nucleotide sequence ID" value="XM_007372409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFS0"/>
    <dbReference type="FunCoup" id="G3AFS0">
        <property type="interactions" value="855"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFS0"/>
    <dbReference type="GeneID" id="18869606"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131749"/>
    <dbReference type="eggNOG" id="KOG0121">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070952_1_1_1"/>
    <dbReference type="InParanoid" id="G3AFS0"/>
    <dbReference type="OMA" id="NENQEQW"/>
    <dbReference type="OrthoDB" id="201398at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005846">
        <property type="term" value="C:nuclear cap binding complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000339">
        <property type="term" value="F:RNA cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042789">
        <property type="term" value="P:mRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006970">
        <property type="term" value="P:response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12240">
        <property type="entry name" value="RRM_NCBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000538">
        <property type="entry name" value="Nuclear cap-binding protein subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027157">
        <property type="entry name" value="NCBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034148">
        <property type="entry name" value="NCBP2_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18847">
        <property type="entry name" value="20 KD NUCLEAR CAP BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18847:SF0">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8 11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="43"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="160"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="160"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="172"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW35059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="209" mass="22776" checksum="92B7E3B972F0FBDD" modified="2011-11-16" version="1">MSHLDLLEDNFKHSAERLDKPSPYLRRKARNNYHELERAITSKTVYVGNLSHFTTEEQIHELFSKCGAIDRIIMGLDRNKLTPCGFCFVIYRTEQGSLNAMKFLQATILDSQSISIDLDPGFREGRQFGRGKHGGQAGQEAAAAVAAAASGGYRGGFRGGHRGGFRGRGRGGFRGGRGGGYPRGEGSVYIPSQDPGFNSQSFGGEGDNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFT3</accession>
    <name>G3AFT3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cation efflux protein transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_131543</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mitochondrial metal transporter involved in mitochondrial iron accumulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372484.1">
        <property type="nucleotide sequence ID" value="XM_007372422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFT3"/>
    <dbReference type="FunCoup" id="G3AFT3">
        <property type="interactions" value="87"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFT3"/>
    <dbReference type="GeneID" id="18869586"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131543"/>
    <dbReference type="eggNOG" id="KOG1485">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013430_12_2_1"/>
    <dbReference type="InParanoid" id="G3AFT3"/>
    <dbReference type="OMA" id="GVYFHSQ"/>
    <dbReference type="OrthoDB" id="435980at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000013">
        <property type="entry name" value="Cation efflux family protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050291">
        <property type="entry name" value="CDF_Transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840:SF15">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0409">Iron storage</keyword>
    <keyword evidence="5" id="KW-0410">Iron transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="13">
        <location>
            <begin position="57"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="13">
        <location>
            <begin position="199"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="393"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW35072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="417" mass="46349" checksum="1D3ABB69754E991F" modified="2011-11-16" version="1">MDTTTTKEKPDAHHIHKESKHTHSHSGAAIFHHHHHSLHEPNQHLLDKTSPASRITWIGLFVNVGLAISKGIGGVYFHSQALIADAIHSVGDMIADFLTLATVNVAAKVGTPTKFPLGYGKIESIGSFLVSAILLFAGISIGWSSLISIFEFVLPSYWYDIVSQVHIGHSHSHTDMLTSSDHGHSHSHLSGLAEPELRPHKVVPNINAAWLAGASIIVKEILFRETLKVANQTNSKVLVANAWHHRIDSLTAVVALLTVTGGVLFNVAWLDSVGGIGVSILIIKAGWDTIKEAWFELIDRGEKPGSELYDKIDEIVRQELLSNEELNKFKLAQLSVLSSGANTNIRMVLHTDVKGIDLKMLNKYEEQLSALIKEDDKFVRNIFIQFKEVDQLEDEHTHEHEHTHDHTHSHDHHDHTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG15</accession>
    <name>G3AG15_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_58341</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372566.1">
        <property type="nucleotide sequence ID" value="XM_007372504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG15"/>
    <dbReference type="FunCoup" id="G3AG15">
        <property type="interactions" value="258"/>
    </dbReference>
    <dbReference type="GeneID" id="18872413"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58341"/>
    <dbReference type="eggNOG" id="KOG0758">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_16_2_1"/>
    <dbReference type="InParanoid" id="G3AG15"/>
    <dbReference type="OMA" id="HICRLRY"/>
    <dbReference type="OrthoDB" id="409586at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF51">
        <property type="entry name" value="CARRIER PROTEIN YMC2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 12" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="15"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="110"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="215"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW35154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="303" mass="32888" checksum="ED8DB594C9B0E6E5" modified="2011-11-16" version="1">MTIELEEPPLTSDNNQVLKDLFGGTMGGIAQVLVGQPFDTVKVRVQSAPEGMYSGSMDVIKQLVKNEGPFAFYKGTLTPLVGVGACVSVQFSVNEFMKRYYDDKLQGKPLSLSQFFVCGAVAGFANGFLASPIEHIRIRLQTQTGSASGAATFNGPIDCAKKLYQSNGFGHGIYRGLVPTLFRESVGLGIYFATYEALIGRELKNNKNMIRAEIPAWKLCLFGGLSGYTLWIGIYPVDVIKSKLQTDSLTKPNYKGSLSVVRDVFAKTGIKGFYKGFIPTILRAAPANGATFAVFELTMRVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG38</accession>
    <name>G3AG38_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mannosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_53501</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372589.1">
        <property type="nucleotide sequence ID" value="XM_007372527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG38"/>
    <dbReference type="FunCoup" id="G3AG38">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG38"/>
    <dbReference type="GeneID" id="18871790"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53501"/>
    <dbReference type="eggNOG" id="ENOG502QW2I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022381_5_0_1"/>
    <dbReference type="InParanoid" id="G3AG38"/>
    <dbReference type="OMA" id="DINVENW"/>
    <dbReference type="OrthoDB" id="409543at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039367">
        <property type="entry name" value="Och1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834:SF1">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="376" mass="43483" checksum="615E9050BA102F8C" modified="2011-11-16" version="1">MNKLLKKIYAVGLITGLYFCLSFHFGSDLQLKSYFQYRPPKVDFLRELKLHPDYKSTGLNFQPKHPGIIPYNTTVLQKLKTLYPYDSAQPIPKQIFKMWKVGLDDPEFPQHFREYHQTWLDNSPDYTHRVMSNDERDALVLKLYADIPEITHAYKIMPKTMNRCDFSSYLLLYALGGIYADIDTKLLKPISSWIPSQSSYLGKSLDLGLIVGIESDYHKGNWARFFARRLQIEAWTFLAKKGHPMLAEIIDNITEYTYWREETGKLAEVLGKDEAADVLNWAGPGRLTDLVFKYLNNILQTDFDSYEKLIDDEFLIGIKLPVVIFDAMVLPLTCMQPGEHSRILQSGNLDDPLAYIHHISAGSWRKHNKGKSIYVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG74</accession>
    <name>G3AG74_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DRAP deaminase and pseudouridylate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_48240</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372625.1">
        <property type="nucleotide sequence ID" value="XM_007372563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG74"/>
    <dbReference type="STRING" id="619300.G3AG74"/>
    <dbReference type="GeneID" id="18871198"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48240"/>
    <dbReference type="eggNOG" id="KOG1919">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016902_12_1_1"/>
    <dbReference type="InParanoid" id="G3AG74"/>
    <dbReference type="OMA" id="THKHEPP"/>
    <dbReference type="OrthoDB" id="424794at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02557">
        <property type="entry name" value="PseudoU_synth_ScRIB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluA-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF40">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE RPUSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="1">
        <location>
            <begin position="117"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="416" mass="48091" checksum="B7AD8BA7BD9196C6" modified="2011-11-16" version="1">MTKYVIENGLRKVKPYYLTYKTPVKLRWCGRTLTKIFQTDFGEREEDIVSDIKNNHLYILSNVGRAGGPTEIKGWDLLQSRQVDGKDVIYHQKHKHEPSVPWVHESIGIAIIYQDSDLVVVNKPAGIPTHATGSYMYNSVVEILKEQLGTDSIWPCHRLDKVTSGVLILAKSKEATCKMSKLIQEKKTSTEKKYLARVKGKFPEGEYVFNCPVFSVNVNGGYLQPPNSHQLQANSTTIFTRLSYNDDLDQSIVLCKPITGKFHQIRIHLRNLGYPIANDFLYNPESESSNEIIKAKNQLELELYQQLFRKYPKFEQLHQLDKYSRTDDEIVDLLEAINWDNDNTIKEALQILKNRQAKKVDEDKNSEQRCTTCNRKLFNVDKAVNPIIWLHALSYSLADDKNSYKFETEYPQWSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG75</accession>
    <name>G3AG75_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AP-2 complex subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_133139</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <location evidence="1">Coated pit</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372626.1">
        <property type="nucleotide sequence ID" value="XM_007372564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG75"/>
    <dbReference type="FunCoup" id="G3AG75">
        <property type="interactions" value="858"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG75"/>
    <dbReference type="GeneID" id="18869724"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133139"/>
    <dbReference type="eggNOG" id="KOG1077">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003824_1_0_1"/>
    <dbReference type="InParanoid" id="G3AG75"/>
    <dbReference type="OMA" id="PVLMHRY"/>
    <dbReference type="OrthoDB" id="28053at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050840">
        <property type="entry name" value="Adaptor_Complx_Large_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017104">
        <property type="entry name" value="AP2_complex_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009028">
        <property type="entry name" value="Coatomer/calthrin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22780">
        <property type="entry name" value="ADAPTIN, ALPHA/GAMMA/EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037091">
        <property type="entry name" value="AP2_complex_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55711">
        <property type="entry name" value="Subdomain of clathrin and coatomer appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0168">Coated pit</keyword>
    <keyword evidence="3 7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin adaptor alpha/beta/gamma-adaptin appendage Ig-like subdomain" evidence="9">
        <location>
            <begin position="719"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="46"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR037091-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="971" mass="109468" checksum="BA134F53D8828917" modified="2011-11-16" version="1">MKGLTQFITDLRNSKDHDEETKKINLEITNIRDKFKTSLNGYQKKKYVCKLIYVYLLGNTEINDFGLKESFDLLRSSVYSEKQLGYLAVSILLNSKRHKPLKDHMNFVVDTLHSDLVRDLQSTNEEFNCLAIQFIASTFSITGTKAFTVYDTDRNADKWLDLIDMTYAAATSPVQQPIVKRKAAITLKVLLEIYPDVILKNSNWIPRLLKLVDDPDIGTAISSIPLLQLVLHLKPQFAISIVPSISQKLYNIIIEGKCPASYYYYNSPAPWLIVKLFQLMEQLFLLTNKDDNQIISISSLDEQTVTYLRQTVARSIQNASQPIKGIPNRNSQSSILFQAVSLAVFLEASTEAINGAMNALLILLNSNETNTRYLSLDALIKLTARSNSTYLSSKDKFDEILPLMLLLLRDRDISVRRKSLDLLYTICTFSNYTTIISKLLDYFPTADFTLKSELAIKIAHLAEKFATDSTWYVTTMLKLLSIGGGSNSNDSNYMGNEVWERIVQIIVNNEDLQKKTAKLIINLVKRSLNRTPQGKAPAPPLSESLIKVAAFVLGEYGDQVIDIEDLNTANQFQLLYEAYFKVSLITRAMILSTFLKFIVNFPDEPFIPDIVDLFEIETQSIDLEIQTRAYEYLKLVTIEPSFKLAQSVIKKNFPIFNTQKESPLMDRLGSVKEIVGRNRSRSVVLAKNINKIDAINEESENPFESESIALSPNWYAGYHRMLHYDAGIFYEDQLIKITYRVIKEAHNITIKFTVINNSYKTVGTQISGFTVLNLESLTKSQDPNYTLHIKSLPEASIYDKTTMEIEVKVRNIVENNESPVLSMTFMCGGSFNHLNLKIPVLLLKTLAPTVLNNTDEFAKRWTQIGDALGIDKGEHKTRIMLAHRYTSSNVSRLLSRVGFAVVFESPEAPDALIRVAGAGIIHTQKSNYGVLISIAGVDQVGRELEITVRCTGGGLAEIVCSTLSEIFSGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGK7</accession>
    <name>G3AGK7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Spt20-like SEP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_69613</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372758.1">
        <property type="nucleotide sequence ID" value="XM_007372696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGK7"/>
    <dbReference type="STRING" id="619300.G3AGK7"/>
    <dbReference type="GeneID" id="18875279"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_69613"/>
    <dbReference type="eggNOG" id="ENOG502QS30">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_377649_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGK7"/>
    <dbReference type="OMA" id="HNYNEHI"/>
    <dbReference type="OrthoDB" id="1932706at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046468">
        <property type="entry name" value="Spt20-like_SEP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47771">
        <property type="entry name" value="LD27203P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47771:SF14">
        <property type="entry name" value="RH73259P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12090">
        <property type="entry name" value="Spt20_SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spt20-like SEP" evidence="3">
        <location>
            <begin position="71"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="13"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="319"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="422"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="647"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="619"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="348"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="459"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="491"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="518"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="656"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="680"/>
            <end position="694"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="694" mass="77457" checksum="339E41004EC0D9E0" modified="2011-11-16" version="1">MLNSGVGNGVSVSQQQLQQLQQHSSQQQQQQQSQHTQLQQSQSQQVKQKIDLQHYHFTSTSEEILKKYARNPASLILHIFETHYRFNNSQDSQIIPKDSPMIKDFMHHVLKEEIPVEMSELLKDFSIKSYDGCLILQVYDHRHMVKTTVTQPRPANASKDTKPVTTIVSKPKTYRTLLKPTPLSIYYDLLYHTDSALTKFTDTLSLQMESEILTLTNRKLDLSVPLNPYACDDYLKPESESTHIEYDEVLDDNKVAFNHRSASSYPARKIHQDELVLHKSSEYEEIMLLLSNRHKRSDEPQDKKLVMVHSSTLPIATAAPVVTNSGKQGSKEASAGVDGKGKKSDKSNIASSAPIPSTLSSTQNTSRSTGQFMRLRLIEEIRKKREAEKLQQEAKIQAQASAVQQTDSIAAAAAAAAAAPVPTPMSAPMGAPESQQVKQPQAPGPQPAAKRVKKEPTKKQLAAQAQAQAKSQQAQAQASLQSPQFNGGMASSTSTSQVQSSSSTPSMRNNQPPPTPVPQQQQQQQQQHQQQMGAPSQGQGQMSLLQQQQQQLFHNSLTPEEQQMFKQMQQKAQAFAIMGNTGITPNRQQLTPQQKQQALQQSKNIQQQLVEKFPVYFQRLRELHAIQQQRQQKQKLQQQRQQQQAQAVAAAAGTNPVDQQHTNIAGKTVPGSASVVPEPKKKRQYRKKNANPTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHJ4</accession>
    <name>G3AHJ4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Alpha-1,3-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_148647</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373742.1">
        <property type="nucleotide sequence ID" value="XM_007373680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHJ4"/>
    <dbReference type="STRING" id="619300.G3AHJ4"/>
    <dbReference type="GeneID" id="18870793"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_148647"/>
    <dbReference type="eggNOG" id="ENOG502T9W7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028276_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHJ4"/>
    <dbReference type="OMA" id="DYWCAYD"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000033">
        <property type="term" value="F:alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392:SF1">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="707" mass="82225" checksum="296700D36A15F56D" modified="2011-11-16" version="1">MFGPRSILRFNRPFVLLFLVLVVIYFWNRPVLNPPSPPFDLIHQRSYFINPIYSKWMIQGPKSRPNIAELSQPQICSQYFHDLSKLYPSGETIVDLEATVAQVTDPLIYKKNSWIRAKESEVKRSSTKRLTAEVEQQLEQDFIRESQKQSNLEKSIVNQFNHMRVFGKCFLAGESDEQESIDNKLCMDFQTQLYPWMSGKFPIYENWKNEKLPQNTVPIFDKSKQIKNSACFIQQFKQNSNGKGIVIPFHPTKGQPRDKQIDNISRIIKVLRALKNRKPIQIVHLDTLTRQEKKLLVDAARTEILYRVVTDVHYPQQNLWFVDVSPVKNTQQHPVMVKQYLFNNINFVVSLSMIFNSFEEAIIMTEHALPLLEKADYFNDQRYKETGLLFFKSPTIYSKRPKRFPPGFHEVTSLVKQFLTPNDFDNHYFGLFKRDDESLATGRFFLDNFQDILDPSIMAINKNIAFSGLLISANFQLHNTLTIRFKTKNNIFTPDFIWVSQEMAGNHNSISFNHNQAVAAGIFTPPSNLPKDVTQSQEICSSSWGQLSDDDDVSLLYVTSHQLQNWRNEGGEHFKELLKDKYKVTSTEMVKNMFNNDENNKVEMERENNEIFDNKLTSNPMTIEAIIRPPTINSPVYVHGYNEPSEAWVEPEGFQEGILHPYYCVYDTVGNPLREGVRGTIVNIGDEMKARLKFLLDVWFEDIQEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHK1</accession>
    <name>G3AHK1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein uS3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">40S ribosomal protein S3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_49231</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373749.1">
        <property type="nucleotide sequence ID" value="XM_007373687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHK1"/>
    <dbReference type="FunCoup" id="G3AHK1">
        <property type="interactions" value="1207"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHK1"/>
    <dbReference type="GeneID" id="18871318"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49231"/>
    <dbReference type="eggNOG" id="KOG3181">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058591_2_1_1"/>
    <dbReference type="InParanoid" id="G3AHK1"/>
    <dbReference type="OMA" id="NKKKWMI"/>
    <dbReference type="OrthoDB" id="10248446at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990145">
        <property type="term" value="P:maintenance of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02413">
        <property type="entry name" value="KH-II_40S_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1140.32:FF:000013">
        <property type="entry name" value="40S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000006">
        <property type="entry name" value="40S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="entry name" value="Ribosomal protein S3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057258">
        <property type="entry name" value="Ribosomal_uS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_uS3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_uS3_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003219">
        <property type="entry name" value="PRK04191.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760:SF32">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="Ribosomal protein S3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="KH type-2" evidence="8">
        <location>
            <begin position="22"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="246" mass="26753" checksum="999D074F5976368C" modified="2011-11-16" version="1">MVNAILSKKKKLVADGVFYAELNEFFTRELAEQGYAGVEVRKTPSKLEIIVKASNTQAVLGEQGRRIHELTSLITKRFKLSPEGVAIYAERIEERALSAAVQAEALKAKLLSGLPIRRAAYGVLRFAMGAGAKGVEVVISGKLRAARAKSQKYADGFMIHSGQPTNDFIDIAIRHVLMRQGVLGIKVKIMKDPATNKFGPRALPDAVKIAEAKDEDEIIPAPTVKVYKQEKVAEEAAEEAPVEASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHU3</accession>
    <name>G3AHU3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59681</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373841.1">
        <property type="nucleotide sequence ID" value="XM_007373779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHU3"/>
    <dbReference type="STRING" id="619300.G3AHU3"/>
    <dbReference type="GeneID" id="18873023"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59681"/>
    <dbReference type="eggNOG" id="KOG4051">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_842406_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHU3"/>
    <dbReference type="OMA" id="FTASEIW"/>
    <dbReference type="OrthoDB" id="5595141at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043189">
        <property type="term" value="C:H4/H2A histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012423">
        <property type="entry name" value="Eaf7/MRGBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07904">
        <property type="entry name" value="Eaf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="330" mass="38423" checksum="5D8E3EE8827DEA31" modified="2011-11-16" version="1">MRSWTTEEEIALFNLVCDHKPAGAVKHKQMDAIIDNINKDVPGSNKFTVADIWAKLATLYDLKRIDYLEDWESESSGESSESETSKKEQDEKIKVEEDKKEDKVKGGKTKEVKIEVPEVEEDEVDEVASPVPEPIKKTTTTRSRSREPRPRRQLRNMTENSGDESSGLSDVESKKEDKEKEEDQESEEEEEEEEEEEQEQEEEEEEEEEEVEQVKEEPKQEEQTKPRRGRRGRKPKAKQNQYRRKEPVRSPNSKKLPSKHHLPNPERRQSQVHSLHAEEHDSSFKKRKVMMTIPKKSLLSKSISRSQLNKLLDVLVEVDELLVRNSISLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI17</accession>
    <name>G3AI17_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TRIP4/RQT4 C2HC5-type zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_134623</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373915.1">
        <property type="nucleotide sequence ID" value="XM_007373853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI17"/>
    <dbReference type="FunCoup" id="G3AI17">
        <property type="interactions" value="104"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI17"/>
    <dbReference type="GeneID" id="18869840"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134623"/>
    <dbReference type="eggNOG" id="KOG2845">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042447_0_0_1"/>
    <dbReference type="InParanoid" id="G3AI17"/>
    <dbReference type="OMA" id="QRDSKVR"/>
    <dbReference type="OrthoDB" id="338816at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180022">
        <property type="term" value="C:RQC-trigger complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039128">
        <property type="entry name" value="TRIP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009349">
        <property type="entry name" value="TRIP4/RQT4_C2HC5_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963">
        <property type="entry name" value="THYROID RECEPTOR INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06221">
        <property type="entry name" value="zf-C2HC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRIP4/RQT4 C2HC5-type zinc finger" evidence="3">
        <location>
            <begin position="61"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="152"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="240"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="365" mass="41622" checksum="7D100A03AAF7BF9D" modified="2011-11-16" version="1">MSKSNPKSKTTSQLLKSKNEDTIIGSGPRLKKKNLANIEEIESILHDLETEAAVTNPKERRYCNCMARKHPLFEIAPNCLNCGKIICTKEGLQPCSFCGADIIPSKDKDEIIKLLAKEREDLLLSPTPMTQSPSTKSKKKIVVKMNTGEKFWEAQDRAFKEAEKELKKKEEALKDEEFEVPVEGSSQTDADLENAQKRLETLLNYQETGAERTKIIDNAADFELPTHTLWLTPEERALNLKKQQRLRQEAEKEKERKLRGERVVEMSIKDGKVTMVEKYVSKKEQASEEELRLQESIKQKKAAEANNGNNQIWDYENDKLKWQKPVYFTTGSEVPVSSPEPLVNAKNRVQFAQEDAEDLIATLIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI23</accession>
    <name>G3AI23_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_149068</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373921.1">
        <property type="nucleotide sequence ID" value="XM_007373859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI23"/>
    <dbReference type="FunCoup" id="G3AI23">
        <property type="interactions" value="227"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI23"/>
    <dbReference type="GeneID" id="18870811"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_149068"/>
    <dbReference type="eggNOG" id="KOG1198">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_16_1_1"/>
    <dbReference type="InParanoid" id="G3AI23"/>
    <dbReference type="OMA" id="CDFSGHV"/>
    <dbReference type="OrthoDB" id="9992527at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08249">
        <property type="entry name" value="enoyl_reductase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047122">
        <property type="entry name" value="Trans-enoyl_RdTase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348">
        <property type="entry name" value="HYPOTHETICAL OXIDOREDUCTASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348:SF2">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C2E1P3.01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="1">
        <location>
            <begin position="14"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="366" mass="40256" checksum="06894783CC3E27DA" modified="2011-11-16" version="1">MTFSSTIKASVISGSLEENQLATVKEIELPPIKPYQLLIKSEAFAVNPTDWKHILYGLGKPNNIIGSDVSGVVVEVGSEVEGFKVGDYVNAFVRGNTSDTEGAWGEYAIINPKVTVKYDHELKHTESNEAGLINSFEGAASVGLGLVTVALSFSHYLNIDYDQAKNKDKHILIWGGAGATGTLAIQIAKLVYGLKVITTASPKNHKYLKSLGADYVFDYNDDKVVEQIKEVGNNKIVYALDTIAFPETFQKVYDATEGAPEVFLDSLLGVDGSVIETKQERVVHYGYTLAYNVLDKEVVLGPVTFKQTPELLANYEEFWKQYLPKYLDQIKHANLLILKPGLESANEALELSKQGKVSGQKVVFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI27</accession>
    <name>G3AI27_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cysteine protease</fullName>
            <ecNumber evidence="11">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_59751</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine</text>
            <dbReference type="Rhea" id="RHEA:67548"/>
            <dbReference type="Rhea" id="RHEA-COMP:17323"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172941"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67549"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase C54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="11"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373925.1">
        <property type="nucleotide sequence ID" value="XM_007373863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI27"/>
    <dbReference type="FunCoup" id="G3AI27">
        <property type="interactions" value="319"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI27"/>
    <dbReference type="MEROPS" id="C54.001"/>
    <dbReference type="GeneID" id="18873064"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59751"/>
    <dbReference type="eggNOG" id="KOG2674">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021259_5_2_1"/>
    <dbReference type="InParanoid" id="G3AI27"/>
    <dbReference type="OMA" id="PDETFHC"/>
    <dbReference type="OrthoDB" id="2960936at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019786">
        <property type="term" value="F:protein-phosphatidylethanolamide deconjugating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005078">
        <property type="entry name" value="Peptidase_C54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046792">
        <property type="entry name" value="Peptidase_C54_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624:SF49">
        <property type="entry name" value="CYSTEINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03416">
        <property type="entry name" value="Peptidase_C54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 11" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="12">
        <location>
            <begin position="66"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="363" mass="40938" checksum="5B62553C70E7E102" modified="2011-11-16" version="1" fragment="single">MSEETAPGDTTTTTNDSIEVVLQTNLNRFTSFVRELSGTTSQENTPDNTEPSPLVVLGEQYTSLEDAEHSISNKLWLSYRCGFDPITKAPDGPTPISFFPSLVFNKRLFTTVRSLFDSENFNSDVGWGCMIRTSQSLLANALMKLQPSAEHEVINLFQDNIASAFSLHNFIRVASESPLEVKPGQWFGPNAASLSTKKLLDGMKGKTIQGVKYPHVFISENSDLYDEEIEELLVESSVLILFPVRLGIDNVNSYYYDSIFQLLACPFTVGISGGKPSSSFYFLGYQDQDLLYFDPHSPQLYENPINYTTYHTNNYQRLHIHMLDPSMMVGILVKDKSEYKEFKRCCTENANKIVHFHPQQSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI41</accession>
    <name>G3AI41_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_133325</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373940.1">
        <property type="nucleotide sequence ID" value="XM_007373878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI41"/>
    <dbReference type="STRING" id="619300.G3AI41"/>
    <dbReference type="GeneID" id="18869739"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133325"/>
    <dbReference type="eggNOG" id="ENOG502S3TP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085417_0_2_1"/>
    <dbReference type="InParanoid" id="G3AI41"/>
    <dbReference type="OMA" id="DYARHDE"/>
    <dbReference type="OrthoDB" id="10249111at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37852:SF1">
        <property type="entry name" value="HIG1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37852">
        <property type="entry name" value="YALI0B21208P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="193" mass="21731" checksum="535FD340A2BB5313" modified="2011-11-16" version="1">MTDTIDVSVQETEVKIDLKKDERFHMYPKDRIYALVVTSAIVGAGVGFYDGIKLASLRYLIENGHRLPTTVGGWYFYHKKKNYVMLVSGVKSGAYSSLKYSAVIGCFFGLEYLLDWSRNSIDFINTTCAAAVLTSTYVATHQLSARQSKKLIFRGTAMGLGLGLMQDFLISQRRGRIWYLEKLGFNSQPKLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI52</accession>
    <name>G3AI52_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein GIN1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">GIN1</name>
        <name evidence="11" type="ORF">SPAPADRAFT_49410</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373950.1">
        <property type="nucleotide sequence ID" value="XM_007373888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI52"/>
    <dbReference type="FunCoup" id="G3AI52">
        <property type="interactions" value="294"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI52"/>
    <dbReference type="GeneID" id="18871333"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49410"/>
    <dbReference type="eggNOG" id="ENOG502QSY2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010084_1_0_1"/>
    <dbReference type="InParanoid" id="G3AI52"/>
    <dbReference type="OMA" id="DQGFQYF"/>
    <dbReference type="OrthoDB" id="422427at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051711">
        <property type="entry name" value="Stress_Response_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47540:SF1">
        <property type="entry name" value="ACTIVATOR OF STRESS GENES 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47540">
        <property type="entry name" value="THIAMINE REPRESSIBLE GENES REGULATORY PROTEIN THI5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="645"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="10">
        <location>
            <begin position="90"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="55"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="740"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="810"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="872"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="810"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="876"/>
            <end position="888"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="973" mass="109664" checksum="9F89A8250CA83129" modified="2011-11-16" version="1">MSKRELPFPNATELVPDGSTFNLSSNSTISNVSNNSNNSSKNTTNLSNLIAGVPVNSTTPTSNNNNYTNISTPKSNKQVSESKRRRVTRACDNCRQKKVKCDGKQPCIHCTVYSYKCSYDQPNIRNKRNSGIPAPAQPSVAVLQAAAAAAAANAANASIENEGNEGAALYPTSNLIVCQQILNILLPKLKFNCFDQNVQFDVERFQKLVSYVETRSPEFTQNINEICDYMQDPEVPIHHLQVHHNLRKASISSNDDTNSITPGTEIKLYLPNKETALQLIYTTWNKGCVLFRFYHRPSLLEEVDLLYSLDPSNYGDRQQRFLPFLYSILACGCLFSRSSATAPDINENSEDDGFKYFLEARKLIDISNVGDINSIQTIVMMIIYLQCSARLSTCYSYIGIAMRSALKEGLHRNLSIFQNSRRKLDPIEIDTRKRLFYTIYKMDIYINSLLGLPRSINEDEFDQELPAELDDENITRTEYLYDKQQGRLSSSGCANHHTKLMLILSHIIKQLYPIKVKNADDELTSSANHYSPDRIHKKVTDLELELKNWLHDLPAELKPTDPNVPGSNKAGEKFVLANYYLHLAFLNCQIMLYRPFIHFISDGNSKGVNSDPRSLIRGRNCIKVARMVVKLANKMIDQNLLVGTYWFSIYTIFFSIACLVYYFHFANYASNRESGVNYAGVLFDDDLNIDMIKQDIEIGKKVLDCLKNSSRSSSRIYNILNTLFEQLNRRTATTSRLRVDGEVPGDANADTNPETVPGARPNVKSEHVQNTFKNFDTINNFESSPSESSYAAYAKRDLGQLFDETNLAQYQSSAEATQSDSSKLEPIHETATTNSEDETNADYLPGVFDKLDTQIFGKILPPYMLESNANYEQNHPQQQQQQQQATAQPIPPQLPPQILQQQLPGQGPMQQMIDPATNPQLQMMAKVDGVISPYDNMGVRSTLNLDDFDFYSINSGDSSGMQYLDPFDPVNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIJ4</accession>
    <name>G3AIJ4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59075</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373293.1">
        <property type="nucleotide sequence ID" value="XM_007373231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIJ4"/>
    <dbReference type="STRING" id="619300.G3AIJ4"/>
    <dbReference type="GeneID" id="18872759"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59075"/>
    <dbReference type="eggNOG" id="KOG1269">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057148_2_0_1"/>
    <dbReference type="InParanoid" id="G3AIJ4"/>
    <dbReference type="OMA" id="WRTIENS"/>
    <dbReference type="OrthoDB" id="10017101at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="37"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="278" mass="31217" checksum="B9958D66CC78CDDA" modified="2011-11-16" version="1">MVQDQAYYSKGYKKEVSNTHAWRTVDNAVPFIIPVIKPNFKVLDVGCGPGTITVDIGANYLTEGGSVIGVEPTQQVLDSAEAHKKKYAEEHNKNLDNILFQTGSIYELPFEDNTFDLVHAHQVVVHLQDPIAGLKELRRVTKPGGFVCVKDSDLESSIVYPEEYQLIQDYYVTKAKSSGCTDPKAGRRLRWKAIQAGYESGNIKTSVSNWLLGDDPNMKHRLNSLIIPRAESGADDLYPDDLEKSKKARSEYIELLKKFADDENASRVVTNYDIVYQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIX7</accession>
    <name>G3AIX7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_48903</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 21 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373372.1">
        <property type="nucleotide sequence ID" value="XM_007373310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIX7"/>
    <dbReference type="FunCoup" id="G3AIX7">
        <property type="interactions" value="282"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIX7"/>
    <dbReference type="GeneID" id="18871284"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48903"/>
    <dbReference type="eggNOG" id="KOG1510">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094271_0_1_1"/>
    <dbReference type="InParanoid" id="G3AIX7"/>
    <dbReference type="OMA" id="LTTYHDH"/>
    <dbReference type="OrthoDB" id="526653at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.10">
        <property type="entry name" value="Mediator complex, subunit Med21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037212">
        <property type="entry name" value="Med7/Med21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021384">
        <property type="entry name" value="Mediator_Med21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13381:SF0">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13381">
        <property type="entry name" value="RNA POLYMERASE II HOLOENZYME COMPONENT SRB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11221">
        <property type="entry name" value="Med21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140718">
        <property type="entry name" value="Mediator hinge subcomplex-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0010">Activator</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="34"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="57"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="169" mass="18767" checksum="1759E3E05DC22337" modified="2011-11-16" version="1">MSDRLTQLQICLDQLVEQFTATVNYVNTNAESALLDPENPTSASNLAANAPLPGHAQPQQLNQTTTQPSPVADNSKVNNEQNEEKFKNTINELSTDIILKSRQISMLIDSLPGIGVTPESQLQLIQELSDELKQVEQERIEKIKQKDQLLAWCEELITAVATGISETRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ54</accession>
    <name>G3AJ54_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_65038</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373395.1">
        <property type="nucleotide sequence ID" value="XM_007373333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ54"/>
    <dbReference type="GeneID" id="18875012"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65038"/>
    <dbReference type="eggNOG" id="ENOG502T68W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1042060_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ54"/>
    <dbReference type="OMA" id="FILHRIF"/>
    <dbReference type="OrthoDB" id="4011634at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="277" mass="32843" checksum="FC826330A753FED6" modified="2011-11-16" version="1">MTGLPIMGKKFLQFLLKRIFEDLDKSLFDKVNLPTIKDCQAGNLIPAKYIVKPPTPRFKNQLSLFNRFFNVTEGFQLTEVNYKYVIHFFEYQYLILKKPKAELLTRIEILDMLCYLAKQIPPEKIVICELKTVLIHMYNKKRFMNEVISFMYKYPILTKYTIDTSLAMCYAFEKFKYPILNDLIKNSKAKDSRSLHDFILFYFHQLMEEQSERLPNLIDIIKKFVNRFIVLPKEVEEQKLVDMLNELSLISLNSSEIISLYGKSLKEFCQRRGTCCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ82</accession>
    <name>G3AJ82_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_59199</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373423.1">
        <property type="nucleotide sequence ID" value="XM_007373361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ82"/>
    <dbReference type="GeneID" id="18872817"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59199"/>
    <dbReference type="HOGENOM" id="CLU_1590437_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ82"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="168" mass="20573" checksum="11E820C7AFD926B1" modified="2011-11-16" version="1" fragment="single">MKYDLTILSTYYGVLNDFKKIEKDFNIDFTEQYISDLFRNGCGQNFEFAKYIHEKYNFNSHSVFYSYLNNGKFSETLNYKYNVLFHTKYPDKLIEYEKLHNILSFYFTLNYDEINFKKLKLDKRFVPTQHYINLGYPDYYNKNITEYCDGENNSMDFIPDRFFYVYPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJJ1</accession>
    <name>G3AJJ1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_48993</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 13">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 13">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="8"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373478.1">
        <property type="nucleotide sequence ID" value="XM_007373416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJJ1"/>
    <dbReference type="FunCoup" id="G3AJJ1">
        <property type="interactions" value="1067"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJJ1"/>
    <dbReference type="GeneID" id="18871293"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48993"/>
    <dbReference type="eggNOG" id="KOG0374">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004962_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJJ1"/>
    <dbReference type="OMA" id="EEHEIRY"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032174">
        <property type="term" value="C:cellular bud neck septin collar"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001400">
        <property type="term" value="C:mating projection base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030437">
        <property type="term" value="P:ascospore formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006873">
        <property type="term" value="P:intracellular monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000370">
        <property type="term" value="P:positive regulation of clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031536">
        <property type="term" value="P:positive regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903501">
        <property type="term" value="P:positive regulation of mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007116">
        <property type="term" value="P:regulation of cell budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005979">
        <property type="term" value="P:regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901901">
        <property type="term" value="P:regulation of protein localization to cell division site involved in cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000004">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF300">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="10">
        <location>
            <begin position="122"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="300"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="328" mass="37687" checksum="61FA21326EB586E9" modified="2011-11-16" version="1">MSTEHQEADIDSIVDRLLEVRGSRPGKQVTLLEHEIRYLCTKAREIFIQQPILLELEAPIKICGDIHGQYYDLLRLFEYGGFPPEANYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFILRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIIDEKIFTMHGGLSPDLNSMEQIRRVMRPTDIPDVGLLCDLLWSDPDKDITGWSENDRGVSFTFGPDVVSRFLQKHDMDLICRAHQVVEDGYEFFSKRQLVTLFSAPNYCGEFDNAGAMMSVDESLLCSFQILKPADKKPRYPPSNVANNRPGATPRKPKKTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK24</accession>
    <name>G3AK24_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Multifunctional methyltransferase subunit trm112</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eRF1 methyltransferase subunit trm112</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_54839</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRM112 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374350.1">
        <property type="nucleotide sequence ID" value="XM_007374288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK24"/>
    <dbReference type="FunCoup" id="G3AK24">
        <property type="interactions" value="857"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK24"/>
    <dbReference type="GeneID" id="18871895"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54839"/>
    <dbReference type="eggNOG" id="KOG1088">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086140_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK24"/>
    <dbReference type="OMA" id="NMLTSKC"/>
    <dbReference type="OrthoDB" id="2187549at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035657">
        <property type="term" value="C:eRF1 methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043528">
        <property type="term" value="C:tRNA (m2G10) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160102">
        <property type="term" value="F:tRNA (guanine(10)-N2)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070476">
        <property type="term" value="P:rRNA (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd21089">
        <property type="entry name" value="Trm112-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000021">
        <property type="entry name" value="Multifunctional methyltransferase subunit trm112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039127">
        <property type="entry name" value="Trm112"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005651">
        <property type="entry name" value="Trm112-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12773:SF0">
        <property type="entry name" value="MULTIFUNCTIONAL METHYLTRANSFERASE SUBUNIT TRM112-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12773">
        <property type="entry name" value="UPF0315 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03966">
        <property type="entry name" value="Trm112p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158997">
        <property type="entry name" value="Trm112p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00069342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00083044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="130" mass="14889" checksum="2B2E93773A337664" modified="2011-11-16" version="1">MKFLTTNFVQCAVKSCHSSLTSFPLQYEECQLVQQEQDYNPEFIVHMLERLDWNAIVQVAKDLGNESLPANKPEDLDPIMEDSQAVLRDLHTLLVETQIVEGKMVCKNCGHIYYIKNSIPNFLLPPHLAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK48</accession>
    <name>G3AK48_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_60200</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374374.1">
        <property type="nucleotide sequence ID" value="XM_007374312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK48"/>
    <dbReference type="STRING" id="619300.G3AK48"/>
    <dbReference type="GeneID" id="18873270"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60200"/>
    <dbReference type="eggNOG" id="KOG1290">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_81_13_1"/>
    <dbReference type="InParanoid" id="G3AK48"/>
    <dbReference type="OMA" id="NEQYFWR"/>
    <dbReference type="OrthoDB" id="5979581at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000275">
        <property type="entry name" value="SRSF protein kinase 2 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051334">
        <property type="entry name" value="SRPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634:SF9">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="392" mass="44658" checksum="5E5A42170703F44A" modified="2011-11-16" version="1" fragment="single">MKLMSKSRYVGGIPLNLVKQIVKQMLLAMDYMHHCGVIHTDLKPENILLDIKDINNIIKAIEVQKLAQYRRRSSDGVAPNGRSRLSRAGTAIFRKNSNAAFSKSRTFSSSSMAHSSSYYRRSKNSINGKHDCPIRTSKPFSSSVTSEVLFTDFQFEESGEKPSSFPMISPRNGSASSLTYPSSVQSTSNIDPEISIKIADLGNATFVNHHFTNQIQTRQYRSPEIILGYKKWGSSTDMWSIGCIIFELITGDFLFDPHDGKYFDRDEDHLAQIIELVGEFPSDEYLMDCKSTSRFFKLKNPNEIVFKNIDSLKYWGLHDVLVEKYKFDKNDVQVKLISDFILKCLKFDLNERYDCGSLLKHPWLQDNVDVNELDLANLPNVHDELPGYTSIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK52</accession>
    <name>G3AK52_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA (guanine(9)-N1)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.221</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA methyltransferase 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA(m1G9)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_60204</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>guanosine(9) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43156"/>
            <dbReference type="Rhea" id="RHEA-COMP:10367"/>
            <dbReference type="Rhea" id="RHEA-COMP:10368"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.221"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.221" evidence="1"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374378.1">
        <property type="nucleotide sequence ID" value="XM_007374316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK52"/>
    <dbReference type="FunCoup" id="G3AK52">
        <property type="interactions" value="778"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK52"/>
    <dbReference type="GeneID" id="18873273"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60204"/>
    <dbReference type="eggNOG" id="KOG2967">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034384_1_0_1"/>
    <dbReference type="InParanoid" id="G3AK52"/>
    <dbReference type="OMA" id="FKKNDGW"/>
    <dbReference type="OrthoDB" id="278300at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052905">
        <property type="term" value="F:tRNA (guanosine(9)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18089">
        <property type="entry name" value="SPOUT_Trm10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028564">
        <property type="entry name" value="MT_TRM10-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038459">
        <property type="entry name" value="MT_TRM10-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016653">
        <property type="entry name" value="TRM10/TRM10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007356">
        <property type="entry name" value="tRNA_m1G_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563">
        <property type="entry name" value="TRNA (GUANINE-9-) METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563:SF13">
        <property type="entry name" value="TRNA METHYLTRANSFERASE 10 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016323">
        <property type="entry name" value="tRNA_m1G_mtfrase_met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51675">
        <property type="entry name" value="SAM_MT_TRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase TRM10-type" evidence="12">
        <location>
            <begin position="95"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="286"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="297"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR016323-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR016323-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW32863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="314" mass="36822" checksum="52336840E80D8182" modified="2011-11-16" version="1">MSTAEPPHPKKKKGNPPTPEEIEERRKLKQLEVIPEGMSKSEWKRQLKSQKWQETKEQYREFQRAKKKAQRQRSKERKRNAEAEEETNYHSIKKSKVMLGKQIPTGVTCIFDCEFDELMNQKEIVSMSNQISRSYSAMRHCEYDLKLKITSFNKNLKKRFDDDVSQYKLWKGIEFNSQQNISDLITEENKSQFVYLTADTDEVIEELLPNHTYIIGGIVDKNRHKQLCLKKAQELGLKVGKLPIDKFIEMNGRHVLATSHVYELCCKWFENGKDWGKAFNEVLPPRKVKGQAKPEEDKGEQEEQEEHSETPSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK72</accession>
    <name>G3AK72_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_65903</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374396.1">
        <property type="nucleotide sequence ID" value="XM_007374334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK72"/>
    <dbReference type="STRING" id="619300.G3AK72"/>
    <dbReference type="GeneID" id="18875068"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65903"/>
    <dbReference type="eggNOG" id="KOG2028">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017271_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK72"/>
    <dbReference type="OMA" id="FANCIEW"/>
    <dbReference type="OrthoDB" id="10265467at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000731">
        <property type="term" value="P:DNA synthesis involved in DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18139">
        <property type="entry name" value="HLD_clamp_RarA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051314">
        <property type="entry name" value="AAA_ATPase_RarA/MGS1/WRNIP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13779:SF7">
        <property type="entry name" value="ATPASE WRNIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13779">
        <property type="entry name" value="WERNER HELICASE-INTERACTING PROTEIN 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="54"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="511" mass="57728" checksum="60E40C9ABA3223E1" modified="2011-11-16" version="1">MTTMDDSPEEHSKPQLPGSLVEAVRPKSLAEYIGQNHLVNPEDGAITNFINLGYLPSMILFGPPGVGKTTLASIIANTCGYVFVELSATDATVAQLRDLSTSIANENAKRIAAGDDMLRILVFIDEIHRFSKTQQDFLLPYIEDGNFVFVGATTVNPQKRIRPAIKSRCQLFQLTTLSSQEMRQVLDRAIDYENTKRRIVYGRGNIVYNDQMLDVLIKRAGGDSRSAINLIELVSTNCRGPEDKQLELAELRDIFQNVRYSQSGIADPKNRQIMARLLACLKGNPGRRKGKDHLEFFYDDFKTRKISGNDPNYEYLLQMQVSDDSDVEPGDIFTDDEEELDLAYLEPEEVAKVSANFYLQLLLKRGESTTVIIRMLIKFAILNLEPNTSTIPKLMSFLKLVKNTNAHQDNIDYIFANCIEWLIDQPKSKSGLQKKLNQLKQYFSLLKKEEDSGNDDDDVPELLNNIIISFNQDEIEGLEHRPIFDEDDIDQNPGISVTYVEEQDLDIGTPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKC3</accession>
    <name>G3AKC3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Casein kinase II subunit beta</fullName>
            <shortName evidence="3">CK II beta</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_60924</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3">Regulatory subunit of casein kinase II/CK2. As part of the kinase complex regulates the basal catalytic activity of the alpha subunit a constitutively active serine/threonine-protein kinase that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the casein kinase 2 subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375096.1">
        <property type="nucleotide sequence ID" value="XM_007375034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKC3"/>
    <dbReference type="FunCoup" id="G3AKC3">
        <property type="interactions" value="647"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKC3"/>
    <dbReference type="GeneID" id="18873636"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60924"/>
    <dbReference type="eggNOG" id="KOG3092">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034027_3_0_1"/>
    <dbReference type="InParanoid" id="G3AKC3"/>
    <dbReference type="OMA" id="QNGSPMA"/>
    <dbReference type="OrthoDB" id="2275560at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030291">
        <property type="term" value="F:protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006356">
        <property type="term" value="P:regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006359">
        <property type="term" value="P:regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000001">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1820.10">
        <property type="entry name" value="protein kinase ck2 holoenzyme, chain C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016149">
        <property type="entry name" value="Casein_kin_II_reg-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035991">
        <property type="entry name" value="Casein_kinase_II_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000704">
        <property type="entry name" value="Casein_kinase_II_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01214">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00472">
        <property type="entry name" value="CASNKINASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM01085">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57798">
        <property type="entry name" value="Casein kinase II beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01101">
        <property type="entry name" value="CK2_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="65"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="255"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="76"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="272"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00045899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW33581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="313" mass="35617" checksum="274B18048D710979" modified="2011-11-16" version="1">MPSDEEDYIPWIQQFCELFGHDYFVQVSQDFIEDDFNLTGLSSQVPYYREALYTILDYQVETAEDSPQDNNSIPGTANTTATTNTANTSSSKKEAKNELPNKALLAHSAELLYGLIHARYIISKQGLTAMASKFERNDFGSCPRYFCDGMHLIPVGSTDIPGQETVRLYCPCCNDIYIPSSSRYLNIDGAFFGTTFPGLLVKMFPEIENQCRIRINKFSANDFGLKLFGFKVNETSASGPRMRWLRMHPKTDEEKEEYENCEFSVPKWVSDSDMEDANDDDQEDGDDLDDDEDDEDDDDEEENDDDDKTMASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKE6</accession>
    <name>G3AKE6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA mismatch repair protein MutL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_150269</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374418.1">
        <property type="nucleotide sequence ID" value="XM_007374356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKE6"/>
    <dbReference type="FunCoup" id="G3AKE6">
        <property type="interactions" value="750"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKE6"/>
    <dbReference type="GeneID" id="18870862"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_150269"/>
    <dbReference type="eggNOG" id="KOG1978">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004131_0_2_1"/>
    <dbReference type="InParanoid" id="G3AKE6"/>
    <dbReference type="OMA" id="SFNNVQY"/>
    <dbReference type="OrthoDB" id="10263226at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032389">
        <property type="term" value="C:MutLalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032139">
        <property type="term" value="F:dinucleotide insertion or deletion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000404">
        <property type="term" value="F:heteroduplex DNA loop binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000710">
        <property type="term" value="P:meiotic mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16926">
        <property type="entry name" value="HATPase_MutL-MLH-PMS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03484">
        <property type="entry name" value="MutL_Trans_hPMS_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000003">
        <property type="entry name" value="DNA mismatch repair endonuclease MutL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.100:FF:000001">
        <property type="entry name" value="Mismatch repair endonuclease pms1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.20">
        <property type="entry name" value="MutL, C-terminal domain, dimerisation subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.100">
        <property type="entry name" value="MutL, C-terminal domain, regulatory subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014762">
        <property type="entry name" value="DNA_mismatch_repair_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002099">
        <property type="entry name" value="MutL/Mlh/PMS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014790">
        <property type="entry name" value="MutL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042120">
        <property type="entry name" value="MutL_C_dimsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042121">
        <property type="entry name" value="MutL_C_regsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037198">
        <property type="entry name" value="MutL_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00585">
        <property type="entry name" value="mutl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF52">
        <property type="entry name" value="MISMATCH REPAIR ENDONUCLEASE PMS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01119">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08676">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00853">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118116">
        <property type="entry name" value="DNA mismatch repair protein MutL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein S5" evidence="6">
        <location>
            <begin position="222"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="MutL C-terminal dimerisation" evidence="5">
        <location>
            <begin position="644"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="388"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="388"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="419"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="836" mass="96836" checksum="EB9F7F1207559F07" modified="2011-11-16" version="1">MSIRSLPSNDILKITSSQVIIDLKSIIKELVENSIDAKSSKIEITFVNYGIDSISIQDNGVGIQQQDFPTLCLRNSTSKLVNFDDLNSLSTLGFRGEALNSICSLCKQLTIVTSTEADYPKNFSLKYNEAGELRDNKSKIGGISNKSGTLVTIEQLFSNLPVRLKNFIKNSKREFHKAINFLINYLLIYTGIKFSVYHINNSKKQLILSSRGGEKTTIIDNLISMFGNNNNKNLLEINLPINEYLKLSGHISSYSFGLGRSTQDRQFLYINRRPIIMKNLSKLINEVYKSFNNVQYPIFVINLEIQSDKFDINLTPDKSMILLHKESEILQLIREKLIEFYNTQDKVIIPRSNNDSFIEQSTFEVNTDEQKSPDEFTLINKVNTKQKEMQEDYRSETHHQENKSETEIEVKHQRAELANENEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEENQREDCEEEINLTNVSQEDKDNSELQDTLFDKVHHKVDHIVSEDFKTVSKSKNENLFLSSDPDSLFSLADSFPKSSDPQSVLLQEPQEISPNQDLHGKNFFPIDENSNDLLEIKIGDKKFEERASKRSRNSSSHLHKLKSYYSSQEFLANLKIKRFQSNTNLKYDQNIKFNNLDDKEKLFHIKKSDFSEMSLIGQFNLGFILVNHQDSNIFIIDQHASDEKYNFEKLVREFQIKTQPLIVPQQLELNIVDEMLIIEHESIFINNGFRLKINHDSLPGTRISMLSLPTYKGTIFSLDDFYELINLINEQPGNTAVKCSKIRKLLAMKACRTSIMIGSSLTSKKMTEIVENLSTLDKPWNCPHGRPTMRHLIELSEWQNNYHDYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKJ4</accession>
    <name>G3AKJ4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nucleoporin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_50460</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375114.1">
        <property type="nucleotide sequence ID" value="XM_007375052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKJ4"/>
    <dbReference type="FunCoup" id="G3AKJ4">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKJ4"/>
    <dbReference type="GeneID" id="18871443"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50460"/>
    <dbReference type="eggNOG" id="ENOG502QQ5B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002415_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKJ4"/>
    <dbReference type="OMA" id="SAKMNPF"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070762">
        <property type="term" value="C:nuclear pore transmembrane ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056541">
        <property type="entry name" value="Ig-like_POM152"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056542">
        <property type="entry name" value="Ig-like_POM152_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056543">
        <property type="entry name" value="Ig-like_POM152_9th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056544">
        <property type="entry name" value="Ig_POM152"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037701">
        <property type="entry name" value="Pom152"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056540">
        <property type="entry name" value="TMD_POM152"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28206">
        <property type="entry name" value="NUCLEOPORIN POM152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28206:SF1">
        <property type="entry name" value="NUCLEOPORIN POM152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24312">
        <property type="entry name" value="Ig-like_POM152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24519">
        <property type="entry name" value="Ig-like_Pom152_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24527">
        <property type="entry name" value="Ig-like_Pom152_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23664">
        <property type="entry name" value="Ig_Pom152"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24097">
        <property type="entry name" value="TMD_POM152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 N-terminal transmembrane" evidence="3">
        <location>
            <begin position="31"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 first Ig-like" evidence="5">
        <location>
            <begin position="177"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 immunoglobulin-like" evidence="2">
        <location>
            <begin position="517"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 Ig-like" evidence="4">
        <location>
            <begin position="728"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 immunoglobulin-like" evidence="2">
        <location>
            <begin position="853"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 ninth Ig-like" evidence="6">
        <location>
            <begin position="1043"/>
            <end position="1117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1228" mass="140637" checksum="E475F7D6A618C20F" modified="2011-11-16" version="1">MDDSDDEVIYLDELRSNKYEDSPLIPTHILDQAQQRIFIVSLFILIQSWKIYDLILIKSDTFVDTQLTQLTSYSFMLKYIILDGLFLWILPVLNIQYLTFTPLKTLLFTFVAYTISWFMISSFGFPLVSNLFLPIWKFFLQKNELNIVGESIDRNKVIDIESHFKGKLTIHYLPDSSAKMNPFHYDKMCLESNSIIPMPIEFNTTSGIGYLQIQQTTADNEVNYVNYTGNSLRKMFRQDYSHLQKYKQFKPDQNIFYLEYPISEPGKYRIAKVLDKKGNNIRTYKSEFMITNCPSVKFFYPANLENVKCMGKDISLDAPWLDSVSTIPSYVRVNVKQDGSDVKTLNITLESQHDLPLKPIQLTKNVLEAELSKYNDLFKTNRRSSNIEFQLLDITDSFGNMKRYNPLSKDKDVWYKLQLKQASTLKLKPMVQDLLVNGELFLKFDNIQQIQDYPIDVTAQFSNSSNMFELSHTFNSRIELEKGFKVNQPGLYKLISAHDNFCPCKIIAEPITVKLALPPSLDISARSVNDKCLGNIGYNFDFNFTGKPPFRVGYQVYHNQSGTLRALGQNRRNLVSNDYKYSFKFKPDDPGSYTIIFHQLTDANYQQVPTILDKKKYSYSTYFNQASQVGFQVQNRKLTTCFGQTSSLPLYFKGNGPFSFTYQFIEVGTSQVLDSKQVNNVEDFTINTPENLIGKSYDVKVSNAKDKFGCDAIIHDKSVRIISRPNIPELEFTNIKQNIRIAEGDSVGLPLKFKSSIGQTGNDKIEVKFSSGDSTKVIPAVMRGNSIIVKQAGNYSLSSFSNNGCPGIISSEKTIEISYYPKPSIEIVANELKQHRDHNTLHLTPICVGCSNEITLKLTGKAPFELDYQIKLPNGKVESHTMNIETDEIKINLPTRRSGIYEHQFMRVFDALYTRRNTAVDKTIPKILYQVNSLPNGKFILDNRLVQICENKLQNGVIATLPVELSGEYPFDIDMILKNEQTGKEQSVQFKSVNEPYLKLLNSQFFSLGDYSVRFSKIVDGNGCEQTKFSANDKFIISITETPDIFKSDNRYHYCIGDHVSYNLTGIPPFTITYQFNSKVKTTQSYYEFRRLSSKAGKLSIQSLQDSGASECKVSFNSDKYSSLELEVHDLPTVEINKGDYIIEDLHQGDQTELIFTFIGTPPFQVTYIRTVEILLNKKPQKKLVEKETIKDIWENEIVIPASLEGTYEAIEVRDKYCQASRAITYIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKK8</accession>
    <name>G3AKK8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7877 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_136419</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375128.1">
        <property type="nucleotide sequence ID" value="XM_007375066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKK8"/>
    <dbReference type="FunCoup" id="G3AKK8">
        <property type="interactions" value="341"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKK8"/>
    <dbReference type="GeneID" id="18869969"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136419"/>
    <dbReference type="eggNOG" id="ENOG502QQT5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043834_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKK8"/>
    <dbReference type="OMA" id="PNWYSLP"/>
    <dbReference type="OrthoDB" id="5354116at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057199">
        <property type="entry name" value="DUF7877"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25289">
        <property type="entry name" value="DUF7877"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7877" evidence="2">
        <location>
            <begin position="24"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="584" mass="66983" checksum="099492F6BEBF301B" modified="2011-11-16" version="1">MFIPVSPIDDEYNTNILTSASSFQKILPDLFTVYEHADKELNVLGSEVLPVEFHEENPFDIVFSLKKRLESKPETTSIQAKFANNEYTKPYQVYHDIKAVASALIQKHARGSKEYRELDFFYKFTTEVLLREVGTLITVNSDGNKSELEHQLDDDFDKIVTTYNLSNGEVITYISKTEEPEPVGYANLYSQHNPVVKQRIQPLFSSIIEKSELDPERTIVPDPFSVAKAVPMIKDVSKSDSILDNLSPVSTKIPTPLESQTSMVLFDFFHPTWYTITVPTWLNYRAQIIKPASMLPQQASQHQNQKLKVLQQRDDTDSTLLSEHSSLVWAPGNNFRSFAPQQDSKDSIISSELKGRVWFNHLGLAEIEKIRSQYLEEKIEDNEEEEEEEEEEEEAEEVPEEEVPEELEQSQKEARITNEVVKGESNEMEIETELDSDATINVANLLQWNPSKIKELKFLKESKQDIINPVKLQRLISISLIKLNKLRQDRYLRSDIRNPLAPEPTEYKLYTRLQKYITMAIQLYKISPSSFSYEFSKRIPVLVSEYSGTLPGVAPNRISTGTSIAKPGRLPSIRGPYKKTKGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKN5</accession>
    <name>G3AKN5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">D-xylose 1-dehydrogenase (NADP(+), D-xylono-1,5-lactone-forming)</fullName>
            <ecNumber evidence="3">1.1.1.179</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">D-xylose-NADP dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60972</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>D-xylose + NADP(+) = D-xylono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22000"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15867"/>
            <dbReference type="ChEBI" id="CHEBI:53455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.179"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfo/Idh/MocA family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.179" evidence="3"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375155.1">
        <property type="nucleotide sequence ID" value="XM_007375093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKN5"/>
    <dbReference type="STRING" id="619300.G3AKN5"/>
    <dbReference type="GeneID" id="18873663"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60972"/>
    <dbReference type="eggNOG" id="KOG2741">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_7_2_1"/>
    <dbReference type="InParanoid" id="G3AKN5"/>
    <dbReference type="OMA" id="CTSSFRY"/>
    <dbReference type="OrthoDB" id="2129491at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047837">
        <property type="term" value="F:D-xylose 1-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050984">
        <property type="entry name" value="Gfo/Idh/MocA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22604">
        <property type="entry name" value="OXIDOREDUCTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22604:SF105">
        <property type="entry name" value="TRANS-1,2-DIHYDROBENZENE-1,2-DIOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="6">
        <location>
            <begin position="5"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="7">
        <location>
            <begin position="152"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00042988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="357" mass="40527" checksum="A42BFDE607690E70" modified="2011-11-16" version="1">MTITLRWGILGAGNISSQFVFDLLLSNERKSETHHVVGSIGCSNKQKGQDFITANKITEANNYGVVPVVQSYQELYDNPDIDIVYIGTPHTYHREQAIGCLNGNKHVLCEKPMTVNKKEAEELFALAKSKKKFFMEAVWTRFFPAVKELRQKVYDEKVIGEVHRLFADFSYYADVTKLPTSSRVRDRTLAAGALLDIGIYPLTYSRILLDDKVGDEHTKFEFKSFMTLDPVDKVDHLSSIIIKYENGKHAILSTSELIDGPKAYIRLEGTKGWAEMYSDNPARAKHFKIFFKDGREPIEYKDESGYNGFIYEANAIAQDISDGKLENDTIPHAETLLVMGVMDQIRKENGLVYDQDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKP2</accession>
    <name>G3AKP2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ankyrin-repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_60285</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374461.1">
        <property type="nucleotide sequence ID" value="XM_007374399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKP2"/>
    <dbReference type="FunCoup" id="G3AKP2">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKP2"/>
    <dbReference type="GeneID" id="18873317"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60285"/>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078327_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKP2"/>
    <dbReference type="OMA" id="TPLHHCE"/>
    <dbReference type="OrthoDB" id="19174at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="34"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="216" mass="24170" checksum="6F12ED262BAF95AF" modified="2011-11-16" version="1">MAINIWVAAADNQRQIVEQYISTGVNTANDKDPNGYTPMHAAAAYGHINLIKYLVENGGNVNIQDNEGDTPLHHAEDLATAKFLVEECGADYTIRNGDNLTAGEYIEDEGEFLDVAQYLKSKQDPSQLVQDDFVSSLPAPGDVEGHQIRYTMENDNSTDETLSPAELEERRKKIQEILQSENPEEGLRELVRNAVHEGLTSFNQQQEEDHSAKRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKT5</accession>
    <name>G3AKT5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_49919</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374504.1">
        <property type="nucleotide sequence ID" value="XM_007374442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKT5"/>
    <dbReference type="GeneID" id="18871386"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49919"/>
    <dbReference type="eggNOG" id="ENOG502RQ1U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_376415_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKT5"/>
    <dbReference type="OMA" id="RQEINMF"/>
    <dbReference type="OrthoDB" id="4087712at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="420"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="539"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="571"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="433"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="572"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="591"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="649" mass="72998" checksum="7A7BFCCCB6FF3058" modified="2011-11-16" version="1">MSLSDPLLQEIEEEFMSPHSSLRRTFALASPVGSFTKESSAGGGTIKSTLERLEVQTICSSQPDSLDSNDPGNSPELTRTTQHSEPQTSLDTFSLLYSDHEEPLPAKYPNTSHINTSPSPKAASMTKKSSESVKSIVNPVPHRFSQFVLDQKKNPLDGISIDSPSLKSDYEYRQGTIKSKPSIQRESIESRLETPPRIIRDISDSMGSQATIFSMRHQDIDNLVPFIQQPEKLLTSSIRQRRRQWFKRNKTQRTSNVRLYTVNSSSSSLHRQNAIKFKEGSWAYRVKLRLKKFLVKLKFFSFTVSSKRTKSFKKIKRTNSTSFKSLIGNPSTNPQLGKTPVFKVAKIDRTTLPITENHLPEVRRSRLSEYINQQENTYLSNLPAKAESIRGSEPTVKYADPQMKSLDPWKAPTAIEEIEAEAEAEAEPPAPAPISVSSSVSATAPPAPPPHTDNSFVETHTIPELWKSYLLQVLANRVKLRQEITMFQKFLVDKDLSKTLNDYFDTESKFSDMRSEMKPSKSSVYVDDASTIQSVATTSNYSSLESESQSEEEFNNKVLNRRSMLGDMLEYSSEEEEDDVDDDNDGDNEPESSPLDTTSRSMSSTSMPTRTLSKTYGTIVRRKPTITRSFGISHSLSDLTDSNEKVYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL30</accession>
    <name>G3AL30_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-conjugating enzyme E2C-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_150678</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374587.1">
        <property type="nucleotide sequence ID" value="XM_007374525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL30"/>
    <dbReference type="FunCoup" id="G3AL30">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL30"/>
    <dbReference type="GeneID" id="18870875"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_150678"/>
    <dbReference type="eggNOG" id="KOG4784">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029122_0_0_1"/>
    <dbReference type="InParanoid" id="G3AL30"/>
    <dbReference type="OMA" id="CKASQAS"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019193">
        <property type="entry name" value="UBQ-conj_enz_E2-bd_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31531:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE E3D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31531">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE E3D FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09814">
        <property type="entry name" value="HECT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="40080" checksum="AB01920152AA5F90" modified="2011-11-16" version="1">MYYTEYLPRLNSISVVLDVPKGFFIDVSTKIILKDPHQLSIQSSQTILDFKLPLPITKFSITNLALQNQVLSFKLVLPSIKPDDSSVSTFMNQLDQKWSCNDLKKTPKSNNNHTFQFVCANCETPLIRSEMYTFKDMPSEYWYEMMDFWHCHKPESDHDHSHDKDYKGVLVPHDDHTVIIGTYYLLQKSNSNITDNVTCAKCGCILGEMIQDNVLKILKWKISLVYADQQERFDSKLFMYNSIIDKINSSATRRFKFKLDNAWKYLWVMNLGVKATLNGDTLVNALKVAFTDKIQEKDEDSYELVDIPYSEIVGSFINDLSSMHSKLPQSINCLTMGPTNFQISLISP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALD7</accession>
    <name>G3ALD7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_137238</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374695.1">
        <property type="nucleotide sequence ID" value="XM_007374633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALD7"/>
    <dbReference type="GeneID" id="18870029"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137238"/>
    <dbReference type="eggNOG" id="ENOG502RQ4I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141134_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALD7"/>
    <dbReference type="OMA" id="VIKDCHY"/>
    <dbReference type="OrthoDB" id="4081497at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="127" mass="14832" checksum="7172F4092159BAB2" modified="2011-11-16" version="1">MSHQDLLTQAKQNQINDLRQSTAYSKQLATTIHTLIRSNSSTSNSLIDKITTIHQLNNEIDRQLNNDLAKSYTELMKYKRRLNKVINECHAIMYHKDVSIINKIQSRVELIDQDLRILEQTLTLVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALE2</accession>
    <name>G3ALE2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_135881</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, it is required to stimulate activity of mtHSP70 (SSC1).</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer with PAM16. Component of the PAM complex, at least composed of mtHsp70, MGE1, TIM44, PAM16, PAM17 and PAM18.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374701.1">
        <property type="nucleotide sequence ID" value="XM_007374639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALE2"/>
    <dbReference type="FunCoup" id="G3ALE2">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALE2"/>
    <dbReference type="GeneID" id="18869928"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135881"/>
    <dbReference type="eggNOG" id="KOG0723">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_13_4_1"/>
    <dbReference type="InParanoid" id="G3ALE2"/>
    <dbReference type="OMA" id="KLMVMNH"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000001">
        <property type="entry name" value="Import inner membrane translocase subunit tim14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763:SF29">
        <property type="entry name" value="MITOCHONDRIAL DNAJ HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442224" description="J domain-containing protein" evidence="9">
        <location>
            <begin position="18"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="10">
        <location>
            <begin position="82"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="142" mass="16206" checksum="CA641DF92878F409" modified="2011-11-16" version="1">MLPIVLGIGATVLALTAKSTITAFSHYSHLTPFMIATLNNIRLVTPNVSPTTSKQDPRYKTYEYIRQKYPNRGFGEPVTESEALLILGIEGEDIMKVDKKMVRDRYRKLMILNHPDKNGSEYLSKRLNEAKDVLDKSYMIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALV5</accession>
    <name>G3ALV5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_60072</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 11">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374229.1">
        <property type="nucleotide sequence ID" value="XM_007374167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALV5"/>
    <dbReference type="FunCoup" id="G3ALV5">
        <property type="interactions" value="495"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALV5"/>
    <dbReference type="GeneID" id="18873208"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60072"/>
    <dbReference type="eggNOG" id="KOG2906">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093932_3_0_1"/>
    <dbReference type="InParanoid" id="G3ALV5"/>
    <dbReference type="OMA" id="MEFCDEC"/>
    <dbReference type="OrthoDB" id="282152at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10509">
        <property type="entry name" value="Zn-ribbon_RPC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034014">
        <property type="entry name" value="Zn_ribbon_RPC11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF12">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8 11" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="12">
        <location>
            <begin position="57"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW32714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="101" mass="11537" checksum="7377B849B99567E0" modified="2011-11-16" version="1">MLLIAPGDDGCNKFYCSTCPYEFKINGLQMYERKKLSRKEVDDVLGGEGTWDDVDQTAAQCPIDSCGGMKAYFFQLQIRSADEPMTTFYKCVKCGHRWKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM08</accession>
    <name>G3AM08_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acyl-CoA thioesterase-like N-terminal HotDog domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_60699</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the C/M/P thioester hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374876.1">
        <property type="nucleotide sequence ID" value="XM_007374814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM08"/>
    <dbReference type="STRING" id="619300.G3AM08"/>
    <dbReference type="GeneID" id="18873531"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60699"/>
    <dbReference type="eggNOG" id="KOG3016">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1623001_0_0_1"/>
    <dbReference type="InParanoid" id="G3AM08"/>
    <dbReference type="OrthoDB" id="68328at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03445">
        <property type="entry name" value="Thioesterase_II_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.210">
        <property type="entry name" value="Acyl-CoA thioesterase, double hotdog domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042171">
        <property type="entry name" value="Acyl-CoA_hotdog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003703">
        <property type="entry name" value="Acyl_CoA_thio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049449">
        <property type="entry name" value="TesB_ACOT8-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066:SF34">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13622">
        <property type="entry name" value="4HBT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA thioesterase-like N-terminal HotDog" evidence="3">
        <location>
            <begin position="44"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="164" mass="18503" checksum="AFB98646085014F3" modified="2011-11-16" version="1" fragment="single">MEQATASDDIVPVDIPSELGVDEIGPNRYIGKKPLRKPDARNRGVYGGSLCAQAILVAMKSSPPGFRPHSLHSYFVKSVDEDTPVVWEVQEISNGRNFVNRMISAIQYDKLVYTANISLTLKNSKSGSKEEEFSFQTPRDENFEKFKSDNLHIYEANPNLYIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM12</accession>
    <name>G3AM12_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_50253</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374880.1">
        <property type="nucleotide sequence ID" value="XM_007374818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM12"/>
    <dbReference type="STRING" id="619300.G3AM12"/>
    <dbReference type="GeneID" id="18871422"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50253"/>
    <dbReference type="eggNOG" id="KOG2071">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_446253_0_0_1"/>
    <dbReference type="InParanoid" id="G3AM12"/>
    <dbReference type="OMA" id="HADEICE"/>
    <dbReference type="OrthoDB" id="2129491at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005849">
        <property type="term" value="C:mRNA cleavage factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16982">
        <property type="entry name" value="CID_Pcf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000016">
        <property type="entry name" value="mRNA cleavage factor complex component Pcf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045154">
        <property type="entry name" value="PCF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047415">
        <property type="entry name" value="Pcf11_CID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921:SF3">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX 2 PROTEIN PCF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="4">
        <location>
            <begin position="3"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="VHS" evidence="3">
        <location>
            <begin position="24"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="354"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="417"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="250"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="364"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="424"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW33365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="533" mass="60879" checksum="A649E71EED7CE9FE" modified="2011-11-16" version="1">MAGDEFDAEYYKSLLNSLTINSRAVITELTSLAEEHIDNSMEITKMIEDRIKRCVPKQKLFAFYLMDSIVKNIGNPYNIIFSQNLFKTFVETYSLVTDTPTRQSLINLFRTWMSGKNSGGGEIFPAAVLAKIEKFIIQATTIQGGGSSIINQTTISDGIPTSKLTPDMLLREGRSLLHYVITLDKALDKKIDENKQYLDKKDEDFKFVHEIEIRRNLLVSIINETMDSVYFDVQGSNDDTKSLGAFTVQNNKFEDHVAKYQTDLKSVRKELDEHSYQQDQFLKKFDVKIKELISKEKLKEEKLKAKQKIFQYLEDNKVVIDYQPDIDYFSNITRDIHKDPKFVSLINRWGKTPVYRQENKSGQNKRDEKQNERDNGNKSEGNSLGFNFGSIDFNELSSNDSEPSSTLLSSSLGSSFLLGSNSNDNDDDDEDQEEQEEENAHDNPLFTRIRPPTPPPRSLTIGSSVEESPKKLSFNDYKQHKSVLASAPIESMATPPPRPPLQHEITVERKSSLKKRSIGGDFNRVVKKVRFDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM72</accession>
    <name>G3AM72_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_136354</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-14 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374292.1">
        <property type="nucleotide sequence ID" value="XM_007374230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM72"/>
    <dbReference type="FunCoup" id="G3AM72">
        <property type="interactions" value="332"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM72"/>
    <dbReference type="GeneID" id="18869961"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136354"/>
    <dbReference type="eggNOG" id="KOG0239">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001485_12_4_1"/>
    <dbReference type="InParanoid" id="G3AM72"/>
    <dbReference type="OMA" id="QDERHAM"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972:SF45">
        <property type="entry name" value="PROTEIN CLARET SEGREGATIONAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="9">
        <location>
            <begin position="289"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="102"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="373"/>
            <end position="380"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="616" mass="70194" checksum="464DAA7D35F881E3" modified="2011-11-16" version="1">MNTPVKRPTTNWSSLVADKSSELTTLKTTVSTLKTQIENSTFSSYEIEHKYHELKRIVRELANQIEELRGYERASLDEINQRFSIEYSKMILDHESRVNAKKEEISDEVEKLIESRTRLYEREISDRESTIKSMRQEIANVDKMFVKKLEQAKQQVKEKEQQLSKEIQESIFRNNEEKEEIYKEVLLMEQEISDNQTAIQDLEAQQTRYTSILEQLKAKHKSKQPELTEITNKINTHKSTIANLASKSQKRSDSTKQIQSELIHINQNLLDHEQIRRKLHAKLQDLKGNIRVFCRIRPLLPGQSPASITVPDDELDEDAKQEIIVARQSSDKLKFSFDKVFPTTSDNTVIFEEISQLIQCSLDGSKVCVFAYGQTGSGKTYTMSHTNDGMIPLSITKIFNDIEELKTHGWEYTIHGQFLEIYNDTIIDLLGSDEGKHEIKHDDTNQTTKVTNATTVQISSKHQAIDLLNHANTHRSTASTKANERSSRSHSIFILSITGHNTRTNSRTSGTLNLIDLAGSERLAVSKAEGDRLRETQAINKSLSNLGDVIHALRQGDAHVPYRNSKLTYLLKHSLGNKTLMFVCVGSGIHEVNETVNSLRFASKVNSTRAVTPTER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM93</accession>
    <name>G3AM93_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60753</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374905.1">
        <property type="nucleotide sequence ID" value="XM_007374843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM93"/>
    <dbReference type="FunCoup" id="G3AM93">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="GeneID" id="18873547"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60753"/>
    <dbReference type="eggNOG" id="ENOG502RQBZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180937_0_0_1"/>
    <dbReference type="InParanoid" id="G3AM93"/>
    <dbReference type="OMA" id="TYRHFAH"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031333">
        <property type="term" value="P:negative regulation of protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010530">
        <property type="entry name" value="B12D"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06522">
        <property type="entry name" value="B12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="72" mass="7970" checksum="7DA2BB3330845192" modified="2011-11-16" version="1">MRPTPALLNAAKKSSGFNLPVELTPLFAAVGVAIASAGFFTYRHFAHDKELRLWKNPDLSTLDEVLDKNTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMP2</accession>
    <name>G3AMP2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UNC-45/Cro1/She4 central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_151669</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375001.1">
        <property type="nucleotide sequence ID" value="XM_007374939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMP2"/>
    <dbReference type="FunCoup" id="G3AMP2">
        <property type="interactions" value="116"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMP2"/>
    <dbReference type="GeneID" id="18870901"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_151669"/>
    <dbReference type="eggNOG" id="KOG4151">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_357916_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMP2"/>
    <dbReference type="OMA" id="CIDENVR"/>
    <dbReference type="OrthoDB" id="5574718at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024660">
        <property type="entry name" value="UCS_central_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45994">
        <property type="entry name" value="FI21225P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45994:SF1">
        <property type="entry name" value="FI21225P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11701">
        <property type="entry name" value="UNC45-central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UNC-45/Cro1/She4 central" evidence="3">
        <location>
            <begin position="134"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="775" mass="88129" checksum="D73C550BDD521034" modified="2011-11-16" version="1">MSQELSDKVSDLSISDNDTLNQFESITPLTEQEQVDLHDKVTKLSQNPQTSKAIYELIINDSSKFLHSLKRIHDTSGVIVTHTIQSTTGSDKIVDNLLSCIRAILKNDTDPESRSYYLGVYSSLVNHFQISNPQHLNLFLTFANSDAALHRSILVLVIQNLKLEKEKTSEIIKEYLEVVCDEKYLDKESFLNFLIVLEMCFPLLPEVCAGVYGDDKTKQHIMSKMELAPDLESMRYILKLIDSSCIVETCRNVNVTNYFQFLVQGCSFKNDQIKVLSSLNLVKLWKFIDAKKKSNDLITTKYLADILISYLKSHTDEYTEYAIEGLMYLTLYWEVRQLLRMDVELIEQLLEKLQQSSTETKDHHVQAFNFPVQYSILSIFANLTKMKDINDTTQQNTANLLKNVATPTSRSENAKEDQNEIKLFNEELLTKYKILDKITKLKTIKGASSHNALNQVMKIIYHFSPQDKLMRVELVKQGAINIVISFLLNSTTVDHKGSIVSSLPNSDNPELIETRVFALRSLARMLIAVDPHLSFKKYDIKTTIPLLKELLGPDISKYTGNVLTNESYLHDMTALDKFESLLALTNIASNASPELKKYIIAQTFDSFLVNFIVEFDDVRIQQASWELIANMITEPILLAKFFNIQQTENKKRLDLVVTLLNSKNVKLQCILAGLLVNATEFDMICEVLVTSEFDHLIGIITEILGGQTNEDDLILPIAYLLVNLVYGAANTSETLLTNLASNTKLKESIAQVLKVVKNREILQPILEVMKMIKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMR4</accession>
    <name>G3AMR4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_66465</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375023.1">
        <property type="nucleotide sequence ID" value="XM_007374961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMR4"/>
    <dbReference type="GeneID" id="18875101"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66465"/>
    <dbReference type="HOGENOM" id="CLU_1278327_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMR4"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442611" evidence="2">
        <location>
            <begin position="23"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="216" mass="24313" checksum="A284B6FCAEE0ABD8" modified="2011-11-16" version="1">MVQCKCLTSFVFLLSLSCPVVSLPVVSPPSEKIRNVSFNYSRNEYLRWEEQAAHLLNYSYSKIEKKNTTTDSSGSYYNITEESNNADVEFVAQGGRGPRWTIGSGVYLHKEVDYGDCSDDDWSDWGRGGWGRRGGRGRGGWGRGGWGRGGWSDDWSDDWSDWSDSDWDGRKFWFLKVRDNDQSSGDIASTANEEPVSESLGNKSNEVMVGNNTKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMS2</accession>
    <name>G3AMS2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription elongation factor SPT5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Chromatin Elongation factor SPT5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Transcription elongation factor spt5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_137836</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The SPT4-SPT5 complex mediates both activation and inhibition of transcription elongation, and plays a role in pre-mRNA processing. This complex seems to be important for the stability of the RNA polymerase II elongation machinery on the chromatin template but not for the inherent ability of this machinery to translocate down the gene.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPT5 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375031.1">
        <property type="nucleotide sequence ID" value="XM_007374969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMS2"/>
    <dbReference type="FunCoup" id="G3AMS2">
        <property type="interactions" value="1356"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMS2"/>
    <dbReference type="GeneID" id="18870083"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137836"/>
    <dbReference type="eggNOG" id="KOG1999">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003537_1_0_1"/>
    <dbReference type="InParanoid" id="G3AMS2"/>
    <dbReference type="OMA" id="YPVGYMN"/>
    <dbReference type="OrthoDB" id="28901at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032044">
        <property type="term" value="C:DSIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033553">
        <property type="term" value="C:rDNA heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140463">
        <property type="term" value="F:chromatin-protein adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001042">
        <property type="term" value="F:RNA polymerase I core binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001179">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070990">
        <property type="term" value="F:snRNP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003711">
        <property type="term" value="F:transcription elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030621">
        <property type="term" value="F:U4 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001208">
        <property type="term" value="P:negative regulation of transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001209">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000232">
        <property type="term" value="P:regulation of rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090262">
        <property type="term" value="P:regulation of transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06081">
        <property type="entry name" value="KOW_Spt5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06083">
        <property type="entry name" value="KOW_Spt5_3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="KOW_Spt5_4"/>
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        <property type="entry name" value="KOW_Spt5_5"/>
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    <dbReference type="CDD" id="cd09888">
        <property type="entry name" value="NGN_Euk"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.30.70.940:FF:000005">
        <property type="entry name" value="Transcription elongation factor SPT5"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="entry name" value="NusG, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KOW"/>
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    <dbReference type="InterPro" id="IPR041973">
        <property type="entry name" value="KOW_Spt5_1"/>
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    <dbReference type="InterPro" id="IPR041976">
        <property type="entry name" value="KOW_Spt5_3"/>
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    <dbReference type="InterPro" id="IPR041977">
        <property type="entry name" value="KOW_Spt5_4"/>
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    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN-like_dom"/>
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        <property type="entry name" value="NGN_dom_sf"/>
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    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
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    <dbReference type="InterPro" id="IPR039659">
        <property type="entry name" value="SPT5"/>
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    <dbReference type="InterPro" id="IPR022581">
        <property type="entry name" value="Spt5_N"/>
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    <dbReference type="InterPro" id="IPR017071">
        <property type="entry name" value="TF_Spt5_eukaryote"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11125">
        <property type="entry name" value="SUPPRESSOR OF TY 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11125:SF7">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF23042">
        <property type="entry name" value="KOW1_SPT5"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF23291">
        <property type="entry name" value="KOW4_SPT5"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF23290">
        <property type="entry name" value="KOW5_SPT5"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF23037">
        <property type="entry name" value="KOWx_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03439">
        <property type="entry name" value="Spt5-NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11942">
        <property type="entry name" value="Spt5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036945">
        <property type="entry name" value="Spt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="NusG-like N-terminal" evidence="11">
        <location>
            <begin position="200"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="12">
        <location>
            <begin position="295"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="12">
        <location>
            <begin position="448"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="12">
        <location>
            <begin position="504"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="12">
        <location>
            <begin position="629"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="12">
        <location>
            <begin position="723"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="789"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="814"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="832"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="850"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="880"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="912"/>
            <end position="941"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006956"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00020181"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00021370"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
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    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW33516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="941" mass="103808" checksum="F306D7D9A8F1E539" modified="2011-11-16" version="1">MSDQTSSGVVKHESSEKLDETLSNDQDLTFDEEFRRADAEEEEDQATLKRPRPEQPEEEEGGNQEEEEDDDEDDEDDEEDEEVTGRKRKRRRGANQFIDIEAEVDDEEEDELDEDDEEAELLREQFITEDHAVDEISKEHVDDRLHRQYDRRRQEAEDQDAEQLAETLKQRYRKTHTVYRGETATSGTVSQKLLMPSINDPSIYAIRCTSGREKDLVRKLYEKKRTLDRSKNPLDILTVFQRDAFRGYIYIEAKRPDAIDKALSGMVNVYIRDKLLVPVKEYPDLLKQVKSTDVELVPGIYVRITRGKYKGDLAIVDNLSENGLDVRCKLVPRLDYGRNDEFDQNGKRIRSKVRPMPRLFSEQEAKMYDNEGLVHRSSRNYLYRNDEYIDGFLYKDFKLQFVQTKDVHPTLEELDRFQTGNEEDGLDLAAIAASLKNKNQADGKQSTAFQPGDKVEIRRGEQAKTVGIVLEASLNEIVITVTDSGDPKFVNQRLTVPPNDLRKIFNEGDHVRIVEGKHFDETGLVIKIDGDSVVLVSDQTREDVRVFANYLVKATDASTNADSANSMYDIKDLVELNASTVGVIVKAEKNIFEILTSDGRLLNVRPSGIASKLKLSRREQVATDRNGLTIKVGDTVRESLGDKKREGVILHIYKNSLFIKSNEIIENLGIFVTNRMNVNTITTKDSIMSKNLGPDLSSMNPNLKLPNPAASVTGFKTHVGGRDKLLYKDVAVTSGSYKGLKGKVTETDDIYARIELHTKSKKIKVPKNNLNVLIRGEAVPYLRFIGASPAQQQQQQPGGYNKPKAPAFSSGSAWNGGGAATGAGSTWGGGASSWQGGKTPSYGSASTWGGAPGGGSGGGASAWSGGKTPGSNSAWGASTGAGDNSTWGGSGGSNSTWGGARKGNNSTWGAGADKGNGSKWGGNGGNSTWGSGGGNSTWGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMW2</accession>
    <name>G3AMW2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">PAN2-PAN3 deadenylation complex catalytic subunit PAN2</fullName>
            <ecNumber evidence="11">3.1.13.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">PAB1P-dependent poly(A)-specific ribonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Poly(A)-nuclease deadenylation complex subunit 2</fullName>
            <shortName evidence="11">PAN deadenylation complex subunit 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PAN2</name>
        <name evidence="14" type="ORF">SPAPADRAFT_138105</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein PAB1. PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. May also be involved in post-transcriptional maturation of mRNA poly(A) tails.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="11">Binds 2 metal cations per subunit in the catalytic exonuclease domain.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="11">Positively regulated by the regulatory subunit PAN3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Forms a heterotrimer with an asymmetric homodimer of the regulatory subunit PAN3 to form the poly(A)-nuclease (PAN) deadenylation complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">Contains a pseudo-UCH domain. This ubiquitin C-terminal hydrolase (UCH)-like or ubiquitin specific protease (USP)-like domain is predicted to be catalytically inactive because it lacks the active site catalytic triad characteristic of thiol proteases, with residues at the equivalent structural positions that are incompatible with catalysis, and it cannot bind ubiquitin. It functions as a structural scaffold for intra- and intermolecular interactions in the complex.</text>
    </comment>
    <comment type="domain">
        <text evidence="11">The linker, or PAN3 interaction domain (PID), between the WD40 repeats and the pseudo-UCH domain mediates interaction with PAN3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peptidase C19 family. PAN2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="11"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375652.1">
        <property type="nucleotide sequence ID" value="XM_007375590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMW2"/>
    <dbReference type="FunCoup" id="G3AMW2">
        <property type="interactions" value="700"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMW2"/>
    <dbReference type="GeneID" id="18870097"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138105"/>
    <dbReference type="eggNOG" id="KOG1275">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002369_1_0_1"/>
    <dbReference type="InParanoid" id="G3AMW2"/>
    <dbReference type="OMA" id="TQELLWT"/>
    <dbReference type="OrthoDB" id="16516at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031251">
        <property type="term" value="C:PAN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006301">
        <property type="term" value="P:DNA damage tolerance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06143">
        <property type="entry name" value="PAN2_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000028">
        <property type="entry name" value="PAN2-PAN3 deadenylation complex catalytic subunit PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03182">
        <property type="entry name" value="PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030843">
        <property type="entry name" value="PAN2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050785">
        <property type="entry name" value="PAN2-PAN3_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048841">
        <property type="entry name" value="PAN2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028881">
        <property type="entry name" value="PAN2_UCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15728">
        <property type="entry name" value="DEADENYLATION COMPLEX CATALYTIC SUBUNIT PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15728:SF0">
        <property type="entry name" value="PAN2-PAN3 DEADENYLATION COMPLEX CATALYTIC SUBUNIT PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20770">
        <property type="entry name" value="PAN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13423">
        <property type="entry name" value="UCH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="9 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="USP" evidence="13">
        <location>
            <begin position="558"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="981"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="983"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="1087"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="1140"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW32376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1182" mass="134182" checksum="220C81066119997E" modified="2011-11-16" version="1">MDGWNEIARIPTTKPTPAWPPNGPGRVRVPDSNPVISALRFDTMQNLIWYGDSFGYTKSLTSSQTNPTLNPYQRSIQLFPYTKFKTCDGINNPVIELLPHKDGILSLSKNQIAFNNRRGIPKFHASAASFHDNLFTNLNSMTFNCNSLNDVVVGTKLSLMKFDLHKPTVLTSFNHDGNVSMVKNTSKLLTIAKGNGSLELFDPVSNTSIKTFNSHNGFISDMDVRGNYIATCGYSLRPKRFHHHGHAPQSDFVVDPLVNIYDTRIMRAIAPIPFPAGATSVRFHPKLPNIVIVSSSTGQIQFVDMFDQTNVYLYQADLSPPHNQMHTLSDSGAASLNNLEISDNGDFLLFNDNYSNLHLWSITNISGVTSKDFVNFPSAIEEPDIPDPTLEENKIGIDDNVPLSCIGMPYYKDLLLSNYASDLKFVKEFARAPEGINADLLLESERNGGRFMLYDKAKYGPGNIVKKFDIVEGKTAIKNKSQIPKFISERNGNGAEKNGTEEIPGIASEIIDESIFQYKSSNSSVIPNCYSRLQIQYSRFGVRDFDFEFYNKTNGLYCGLENHTDNSYINSLLQLYRFSPVFYNKIVKNLLNEWLPCDLDTILVNTEGSSILTELGYLFDMMDKAKGNNVKIYNFAQVVTHNKDAIAHHLINLDEMNHLNSLDVRNLIISFNKFLLSQINSDLHVQSQDSIEEIMKLTYMVEVRGNESTCPVYDKSYGSILSLDLVTPPSNMLNKMSILINPHLNTTAAQQPPQLANIRRNVNILTYLEYSMNQVKLVPCQQHQHAYPHSLEIKTTITSLPPVLVLNVNLSNEEFQLINGFRKWIIPEFYAVNQKDKFSFKSMISPFDKANSAKYDLLGFVCEISHSSDFALGMHGLVTFVKLDQWYLFNDFLVMPIPEEEVLDLSSPWKKPVVVIYQESSIAQTPFEYFNIETFGKLPNLNDSILYRDHFAGTIREGYKKEYELLTKQEAPKADTLVAIDAEFVLLNPELLEVHYDGFKKLIKPKILSLARVSVLRGEEETAFIDDYVVHTAKIHDYLTSFSGIEDSDLDPIRSAKNLVTLQTAYRKLWLLLNLGVVFVGHGLYNDFRCINLQVPKKQIRDTAEFYYKSDFKRQLGLKFLAYVLLKEKVQSNNHDSIEDAYTALMLYKKYLELTSQGMFESTLNFIYSEGQQMRFRVPEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMX1</accession>
    <name>G3AMX1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">High osmolarity signaling protein SHO1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">High osmolarity signaling protein sho1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12 13">Osmosensor SHO1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_50946</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Forms homooligomers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SHO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375662.1">
        <property type="nucleotide sequence ID" value="XM_007375600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMX1"/>
    <dbReference type="FunCoup" id="G3AMX1">
        <property type="interactions" value="156"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMX1"/>
    <dbReference type="GeneID" id="18871502"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50946"/>
    <dbReference type="eggNOG" id="ENOG502QW7A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043316_1_0_1"/>
    <dbReference type="InParanoid" id="G3AMX1"/>
    <dbReference type="OMA" id="NIVWIFY"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007232">
        <property type="term" value="P:osmosensory signaling pathway via Sho1 osmosensor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11855">
        <property type="entry name" value="SH3_Sho1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000213">
        <property type="entry name" value="High osmolarity signaling protein SHO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035522">
        <property type="entry name" value="Sho1_SH3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF20">
        <property type="entry name" value="HIGH OSMOLARITY SIGNALING PROTEIN SHO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="10" id="KW-0346">Stress response</keyword>
    <keyword evidence="8 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="234"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="196"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="196"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW32386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="295" mass="32566" checksum="435A32E80D3CE419" modified="2011-11-16" version="1">MGYSFSNLTGDSFGIATISFGIISWVVSIAGAAASNQKNFPNLSWWGLAYEAVIILVVLLLYCNNNIELYKFTLVGLIAIAFVYSTNTVNNLIYNTNSSANLTCAAGCILLSILNLLWILYFGGHPESPTNQFIDSFSIRNQGPTHDNLNRAINKSEQYNGVEELGAMGEPTATTTTYNQRFSSVASARPASKQYMSSSQLNGLENSSDIPGRDLTQSKRNTMYPESELGTGVTFRYKAKALYSYDANPSDENEISFKKDEVLEVDDIDGKWWQARRPNGEIGICPSNYVKLIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN41</accession>
    <name>G3AN41_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_61523</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375731.1">
        <property type="nucleotide sequence ID" value="XM_007375669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN41"/>
    <dbReference type="GeneID" id="18873935"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61523"/>
    <dbReference type="eggNOG" id="ENOG502R8TB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1653218_0_0_1"/>
    <dbReference type="InParanoid" id="G3AN41"/>
    <dbReference type="OrthoDB" id="4024240at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="160" mass="18303" checksum="4E3079028A7C52DD" modified="2011-11-16" version="1">MTEKYVFAITNFTKLTKLSLDVIQESTFVRTGRVPQSLYSGISQLANLTSLRVWFGGSSHDDLPHLVGKLPNLKYLDIPRITTSLPPFLQYNRMIPGVYFDDMYELRSNALYLFKSNASIKFVRINSSIFECCDGANVVSRDGISRWFDERVRYISELGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANC0</accession>
    <name>G3ANC0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF1751-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_138847</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375780.1">
        <property type="nucleotide sequence ID" value="XM_007375718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANC0"/>
    <dbReference type="GeneID" id="18870156"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138847"/>
    <dbReference type="eggNOG" id="KOG2890">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_676370_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANC0"/>
    <dbReference type="OMA" id="YYTYFVL"/>
    <dbReference type="OrthoDB" id="203724at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000004">
        <property type="entry name" value="Transmembrane protein 115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013861">
        <property type="entry name" value="TMEM115/Pdh1/Rbl19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13377">
        <property type="entry name" value="PLACENTAL PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13377:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08551">
        <property type="entry name" value="DUF1751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01160">
        <property type="entry name" value="DUF1751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW32504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="355" mass="40544" checksum="EA046579A231FA04" modified="2011-11-16" version="1">MAVPKVTSILLVILITLTLLNFILKYYTYFVLIVASSKSTPNPDTPESTSIPHPHELFVPLLTFIPSKSTVLAYPWVLVTASFVEETFIGLFVSIVGMFYLGRYLENVWSSPEFLKFVMFNVIVTNSLLYLYYSFKSWFIESEVPVVLSSMGIIMGFIVAIKQRIPNHFFILFKGNLRIKVKYMSFILIILSLVLAFLNSEYYITYLMCCYGFIISWFYLRFVKTSLNEGQSYLIPFSKTSPIEPKEEEAKQSDQFALHTFFPYPLSTIIQLVSSIGFKLAIKYKLISGKEHTLSSGLDEQFNSKLFAVSSLKGVKDVGVLNISKVFHWFSSSGSTGINNTMDKRRKMALKEIDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANC8</accession>
    <name>G3ANC8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA exonuclease 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_55944</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Exoribonuclease involved in ribosome biosynthesis. Involved in the processing of ITS1, the internal transcribed spacer localized between the 18S and 5.8S rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the REXO4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375787.1">
        <property type="nucleotide sequence ID" value="XM_007375725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANC8"/>
    <dbReference type="FunCoup" id="G3ANC8">
        <property type="interactions" value="810"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANC8"/>
    <dbReference type="GeneID" id="18871989"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55944"/>
    <dbReference type="eggNOG" id="KOG2249">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022453_2_0_1"/>
    <dbReference type="InParanoid" id="G3ANC8"/>
    <dbReference type="OMA" id="RYKPLCK"/>
    <dbReference type="OrthoDB" id="8191639at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06144">
        <property type="entry name" value="REX4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000007">
        <property type="entry name" value="Interferon-stimulated exonuclease gene 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037431">
        <property type="entry name" value="REX4_DEDDh_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF45">
        <property type="entry name" value="RNA EXONUCLEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exonuclease" evidence="11">
        <location>
            <begin position="100"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="273" mass="30899" checksum="FE08266B430A7FBE" modified="2011-11-16" version="1">MAELSSNWKKLSSKISKPKTKKKTTTKLTSNAKKVISKPTKVETTPKQIEPKSDTVTNTVSPIEYALWTKEHITKEDLPSAPVALTHTPSSARRKQEPGKYIAIDCEFVGVGDDGERSVLARVSIVNFYGHILIDEFVKPRERVTDWRTWVSGVTSKHMHDAITFEEAQKRVADLIKDKIVVGHAVHHDLDSLLLSHPGWLIRDTTSYPAFRKIANGRSPSLKKLTGHFLGVDIQQSSHSSVEDARATMLLFRLHKKDFEQHLRNRHRKVSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANP7</accession>
    <name>G3ANP7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61094</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375257.1">
        <property type="nucleotide sequence ID" value="XM_007375195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANP7"/>
    <dbReference type="FunCoup" id="G3ANP7">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="GeneID" id="18873708"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61094"/>
    <dbReference type="eggNOG" id="ENOG502S36W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1343113_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANP7"/>
    <dbReference type="OMA" id="NCMVSAP"/>
    <dbReference type="OrthoDB" id="4067208at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="70"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="166"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="234" mass="25478" checksum="03852110E0ACFF0A" modified="2011-11-16" version="1">MSTVAASSSNQAEQQFVNTYLDFLSLSKDASPDQFNSTQDYDKIESLGPSLPPYKYKFPKVVSATSERTLTLKFKSIKPPFKFTSELHDIPESFTIHRVKEALIKAQDVLNQAGAKPDNLKLMLKSKVVQDSSTLGDVSPEDEVSFNCMVSAPIATTKPEEPTNVTSAGTVASSGATSSTTTTPSSTHSTTTTTTKELDGISPEAWQQIYSVLLQDLNDQQKAQDILSKFKQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANR7</accession>
    <name>G3ANR7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_152327</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375278.1">
        <property type="nucleotide sequence ID" value="XM_007375216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANR7"/>
    <dbReference type="GeneID" id="18870925"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_152327"/>
    <dbReference type="eggNOG" id="KOG1208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_6_1"/>
    <dbReference type="InParanoid" id="G3ANR7"/>
    <dbReference type="OMA" id="KPDGVNW"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF236">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="357" mass="40078" checksum="5E7F11AF8B3C249B" modified="2011-11-16" version="1">MCISNLYKRRELVKILSTEQIYITMSAPVFPSRTWSDYKQFKNGFLPGKPTFKEDHYPSLKGKVVLITGGNSGVGYETVKSLAGSTKAKIYIFSRNEKKTLAAIRQIRQEVAQEYNVTNPDINFIRMDLSDLDTIKPAVDQFLSQEARLDIIIHNAGVMKRPIGPKTRQGYELQLGTNVVGPHLLQRLLDPILIDTSKMNHPGESRIVWISSTAHHFAPLGGVHWDDINFDHTKIGKLYNVMGYGQSKACNIMQARTWSRKHSAGSQIICSSVCPGFLNTQLARNSSCIERAIFSVILHPRRLGAYTVLYAAFSPEVEDGSFSVSFGVPSQPRKDLTQDDACDKLWDFLTKATDPYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANR9</accession>
    <name>G3ANR9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_139460</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375280.1">
        <property type="nucleotide sequence ID" value="XM_007375218.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3ANR9">
        <property type="interactions" value="398"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANR9"/>
    <dbReference type="GeneID" id="18870210"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139460"/>
    <dbReference type="eggNOG" id="KOG0161">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000192_4_0_1"/>
    <dbReference type="InParanoid" id="G3ANR9"/>
    <dbReference type="OMA" id="DVRFLHK"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000142">
        <property type="term" value="C:cellular bud neck contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032033">
        <property type="term" value="F:myosin II light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902404">
        <property type="term" value="P:mitotic actomyosin contractile ring contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031671">
        <property type="term" value="P:primary cell septum biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904498">
        <property type="term" value="P:protein localization to mitotic actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000920">
        <property type="term" value="P:septum digestion after cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01377">
        <property type="entry name" value="MYSc_class_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF857">
        <property type="entry name" value="MYOSIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 8" id="KW-0518">Myosin</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="10">
        <location>
            <begin position="72"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="8">
        <location>
            <begin position="624"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="815"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="927"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1241"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1366"/>
            <end position="1475"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1531"/>
            <end position="1663"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1720"/>
            <end position="1802"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="163"/>
            <end position="170"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1861" mass="214305" checksum="63A70A703CD92704" modified="2011-11-16" version="1">MDSTSKNWVWIPDPDQLFVKGYVSDYLDNNKCKVIIDPTAGGGGAAAAATTSDNHRIVDQSTLESCNPDKFNKCNDMAELTHLNEPSVVYNLYLRYMDDLIYTYSGLFLVAINPYKNLPIYDRGSLKHHHQSDKPQPHIFSIAEAAYRNMLSNKKNQSILVTGESGAGKTENTKKIIQYLSLVTSSEGEASDIHSKIIQANPILESFGNAKTIKNNNSSRFGKFIQIYFSSINGNITGATIEYYLLEKSRVVNQASNERNYHIFYQFLKGYDNLASLGLNKDITTYNYLNNSSIDQVDDFKEFNLLQQAFTIMGFSDEETQFIYQVLAIILHLGNIDFTSWKSDQANFTPESPLAMISQLLGIKKEELVENLSRPKVQAGREFIVKSKRPSEVKFIIDAFAKYLYERLFQFIIDKINTGLHTTNPQYDNNHNLIGVLDIAGFEIFDVNSFEQLCINYTNEKLQQFFNHHSFILEQSEYLREAIEWDYIDFGQDLQPTIDLIETKTPMGIFKLLDEECIMPKSSDESFMEKLSQNFNKSHPKFSENKFKNGFIVHHYAGKVEYNVTNWLQKNTDPVSESIIKLLPHSSNSFIAAMFGSDPHLEVASKRISNFKLKTASQKHKDQLKTLMDQLSITEPHFVRCILPNLKKSPQMFDKQLVVNQLRCNGVLEGIRITRAGYPNKMTFDDFNARYAILHDLELRHEPRETSEMILNHTGLDKQVYKVGLTKIFFKNGILAKLEELRDCKLKAIFTDLQKIIRGNAKRRMIKHQIRQIQSAKIVAQTMQKVEEAKSGSYWMELFYHIKPLLEESGKVLDTREIQDNLNKVTEQLKETEGTKQGLEKDNQILKTQVSKLEDEIISTASVMNEKNGQLDKLKSEAIKAKATIKDLEEKVTSMAHVNKEIIDEKDKLNTRLFELSASHEGKIKELESLSNLHTSAKDELGRLKRELKSLAESKSKELTNLKQSHESKLSDSTREVGRLNQLIADLKKTHETASKSEHQALVDEITTLKSTISKLNSEIATYQSTIKDLNSKIEKNESYKTKYDAKIDEAKEKVHLLKSKVETKTSEIAQQKQQIDQLTRELASIKDKLALSENKLTELNQVKDAQSKNLSEIERLTEQLTQADQQQQEIQAEYDKLKQEHAKLEQQANDQITFLNSKLAQLETQEKENVPEATPSIMEEYKAMKLKLNESSAMLRREKFENRKIAEELSILKERVNGSSKVDVKRRSVAYGEKTDSAGLDVLHREIEDLKFKLSQEQGNFARAETYAVELQKKLNKLQATRGLNSVTDYEKKYQESEKRARDLESKINELVVHSNSPPTSADNSFTRSDSIMSLSNHALRGVNADFVQIYQDISKTLRATREELNHSKSEILRLKSLLRDSEEELYAAKSNNFRTSISDYETKIANLNVKYENLLSRNKDLNDGVELYKKRAEEYFQKLELAESAVTISKRHEEASRRELNEAKSQLILAREESRASQMLLKEFQGKVGTLETLAQEKKFQLGKAHDEIKILKEKIDYHVNNYENKEVTEQLKEEINNLHKELNFKTETETSLIKENKQVQLDYEDMVRTKNEMETELGEALVREDKLEAQVQTLTNSVRQLEDDKFINERKIANFTKQTASLKSLIEEVTTQRDSLVQEKEELGDQVYRLTRELDDKTAELSQTNAKLDLMRTHLQNQRDDSEQIKTELNQSKISSTSEYMDQQRLRKELLVTNEENLSLKKANSELNSKVSDLQQKLYSNEQLQFWETKVKDLSSQLDTAISEGHEANKIIKSLERNVKQLEIRVEHETQLTKRYNDENFDYQNKVNHYKSTIEILHQENIDKDLALKTSERANSEMRGELLLAQKEILELRQRLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANT0</accession>
    <name>G3ANT0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Arginine N-methyltransferase 2</fullName>
            <ecNumber evidence="6">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_61117</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">S-adenosyl-L-methionine-dependent protein-arginine N-methyltransferase that methylates the delta-nitrogen atom of arginine residues to form N5-methylarginine (type IV) in target proteins. Monomethylates ribosomal protein L12.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. RMT2 methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375291.1">
        <property type="nucleotide sequence ID" value="XM_007375229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANT0"/>
    <dbReference type="FunCoup" id="G3ANT0">
        <property type="interactions" value="416"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANT0"/>
    <dbReference type="GeneID" id="18873722"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61117"/>
    <dbReference type="eggNOG" id="KOG1709">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033831_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANT0"/>
    <dbReference type="OMA" id="YWVVDNY"/>
    <dbReference type="OrthoDB" id="19014at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019702">
        <property type="term" value="F:protein arginine N5-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000310">
        <property type="entry name" value="Arginine N-methyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017408">
        <property type="entry name" value="Arginine_N-MeTrfase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051038">
        <property type="entry name" value="RMT2/GAMT_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026480">
        <property type="entry name" value="RMT2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32379">
        <property type="entry name" value="GUANIDINOACETATE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32379:SF1">
        <property type="entry name" value="GUANIDINOACETATE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038148">
        <property type="entry name" value="Arginine_N-mtfrase-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51559">
        <property type="entry name" value="SAM_RMT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RMT2" evidence="7">
        <location>
            <begin position="175"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR038148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="412" mass="47053" checksum="C48D5333F5645670" modified="2011-11-16" version="1">MSDLHDLCRFSTRPIDESYITQLHHYLKSGIPATYTVEEAANYINGIEETPSTTTTPLHLICMNVPLGCTDEELEIVEKMIEILLEYGAGWCFTDVNNETPGCILIRRGLKHVGKLYSQIVDAGVRAELLLRKVSDYDVEFIDPEDVEAQQNETVESEVVVESKDSEIDDAPDAPSQNQQSYLNTKLEYVDDALVTKDRKDGVMMAWESDLMKLGCDSLFKGAILDGDTKEMDSEIVVLNIGFGMGIIDTMIQHQHPTKHYICEAHPDVLKKLKDDGWYEKPNVVILEGRWQDNLDELLSQGVFFNGIYYDTFSEHYQDMLELFDFVVGLLKPHGVFSFFNGLGADREVIYEVYKQLVEIDLKTYGLDVKFTEVEVPESTLKSNDKSVWDDVKRSYWSCPVYYHPEARFIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANT5</accession>
    <name>G3ANT5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Plasma membrane proteolipid 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_61122</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375296.1">
        <property type="nucleotide sequence ID" value="XM_007375234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANT5"/>
    <dbReference type="FunCoup" id="G3ANT5">
        <property type="interactions" value="698"/>
    </dbReference>
    <dbReference type="GeneID" id="18873726"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61122"/>
    <dbReference type="eggNOG" id="KOG1773">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107649_5_0_1"/>
    <dbReference type="InParanoid" id="G3ANT5"/>
    <dbReference type="OMA" id="HALWVIS"/>
    <dbReference type="OrthoDB" id="2802411at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659">
        <property type="entry name" value="HYDROPHOBIC PROTEIN RCI2 LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN LTI6 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659:SF112">
        <property type="entry name" value="PROTEIN SNA2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="80" mass="8901" checksum="9372DBA5149A493E" modified="2011-11-16" version="1">MHARDWLLVFVGFFFPPIPVIVKKGFCSADLLINILLCILGFFPGLLHAYYIIAMNPHQENYIAIGSGEQRVGDYGAISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANV1</accession>
    <name>G3ANV1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_55986</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375852.1">
        <property type="nucleotide sequence ID" value="XM_007375790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANV1"/>
    <dbReference type="STRING" id="619300.G3ANV1"/>
    <dbReference type="GeneID" id="18871993"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55986"/>
    <dbReference type="eggNOG" id="ENOG502T0CG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025334_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANV1"/>
    <dbReference type="OMA" id="CAPMIAM"/>
    <dbReference type="OrthoDB" id="4084092at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="690"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="574"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="517"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="715" mass="82729" checksum="02788D6CEF5577FD" modified="2011-11-16" version="1">MNVTSTGYYTSSYLNQQMSKRRQHIPYDQYYSSYYHNDIEDNSIRDEEIKTILSSPKSRAVSEKPRVPSKTSLYSQSSSPPSSSKSSRPTISAYSSDSSLGTSEMFVADDDDELESEEESDDPDRLAEELTPAHLNYLATQPQYVPYRPSRRDPYQPQPLSPVQQIHYVARPPRVRPNKNGDYYINQQKKYDKASGDMKPRLYSHKTFKDVFINKDEKLDRYNPMDLVFEGRDANANGNKFLKNVQTILKKDDYNDYNYYDHKTKEKKKKSPVKEVFVSDVEDDDSDIESIDQETFLNEEQRKMIKKNSTLKKAWKKKIAKAKKELGRDFDKFAIKQNEIEHELKEKKEKEKAAKKEAKEKEKAAKKGKKEDKKEEAKDQEKEEKKEDKKEEKKEEKKEEKKEEKKEEKKEEHKKADDKFSPGWNYLLSLVKYDRPTEPNKEKEEKEAPKPKPKPAPVMESPYTKNTRIIKKNLKGFTKNSKIIFGNWNQPVANVLTGELAKTDKKKVLKPASVKAKSVHSKSIHSKSIHSKSIHSKSVHSNEEGDDDVESKEFVIECDSDNSDEDIAEELYYNPITKQLEPTPPTSSSSMEVDDVDSDSEAASVHRLPLMYQTMTHARSLAGRFFTLDPHTPLAVVSNFVSLIKKIDIMRRVFSPIDIMGEWFPQAQTLVIFLELGIFMWILFELSRLIDALCMMIKAFCAPMIAIGRFMNKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP20</accession>
    <name>G3AP20_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61145</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375327.1">
        <property type="nucleotide sequence ID" value="XM_007375265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP20"/>
    <dbReference type="GeneID" id="18873741"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61145"/>
    <dbReference type="eggNOG" id="ENOG502SVZ2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_744271_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP20"/>
    <dbReference type="OMA" id="RMVWNVL"/>
    <dbReference type="OrthoDB" id="4096984at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="372" mass="43402" checksum="B2BE517F5BA36AC8" modified="2011-11-16" version="1">MRFIRLYNISHHPLNYHTYLSRIKKLPKVYHESTTPIKQQLLLDLVKANRTLQRTNTSPNYAPTRLNLELSTPTNAKLESGLKKLLFHDFRRDALDDVQVVKEYLLTEPKPSNVQDLIEITSDSFRNQLTHKKDVDTSIVYITLSRILDQNDYFNGFNLIDKTVNSDLYLDYRKSRLMKLVVSATLANIGVLALQSTLLPDIPLLAWMLLNISSTALVSSMANLKISNLGRIKFRFYNSQLYNYLHKHELITTNKIITHLEEFNETNLINFHYSKVRPEPNLKTFTVDNYIVELPDAVVETNAHPWTHLPNEEISNIQKFFKSELNKRRMVLCDLNSELLFLEYWKNHGEGFAWVEPDQDPGEIIKLKIKQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3APH8</accession>
    <name>G3APH8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Maltose permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_61232</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375424.1">
        <property type="nucleotide sequence ID" value="XM_007375362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APH8"/>
    <dbReference type="GeneID" id="18873789"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61232"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_11_0_1"/>
    <dbReference type="InParanoid" id="G3APH8"/>
    <dbReference type="OMA" id="LEPYFMN"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000078">
        <property type="entry name" value="MFS maltose transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF83">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="325"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="354"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="1"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="420" mass="47095" checksum="1080A8B6FC4DA163" modified="2011-11-16" version="1">MVINVSLIFMAAFIFIVFFARSIEVLVVGQVLCGIPWGIFATIGISYASEITPLVLRGYLTAIINLYWATGQFIAAGVLQGLVGLDSKWSYKIPYALQWMWVVPLFVLTYFAPDSPWYLIRKGRISDAEHAIKRLGSKRIAHKAKDSLALMIRTNEIEVQQDRKNREELGGWKGYIECFRGTNLRRTEIMCISFAGQVLSGSTFAYSPSYFFSQAGLNSDQVYKLNLGTTGIAFSGTVCSWFLLNKFGRRTIYVTGYFFLVLFLFLIGVLAKPAETNGAVKWAQAALTMLWVSTYALTIGPLAFTITAETSATRIRSQSVCLARNAYNLVSLISQIVEPYLINPGSANLKGLTAFVWFATAFPTLIWSFFRLPETKDRTYAELDILFERRVPAREFAAYQIDVADIVQETVSEVPVTEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3APQ8</accession>
    <name>G3APQ8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_138221</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375505.1">
        <property type="nucleotide sequence ID" value="XM_007375443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APQ8"/>
    <dbReference type="GeneID" id="18870105"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138221"/>
    <dbReference type="eggNOG" id="ENOG502T5UX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142633_0_0_1"/>
    <dbReference type="InParanoid" id="G3APQ8"/>
    <dbReference type="OMA" id="GHKRDDN"/>
    <dbReference type="OrthoDB" id="4019261at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="156" mass="17418" checksum="0A7E1DAA8A1A8971" modified="2011-11-16" version="1" fragment="single">KRDLVDKMLVRVFHSLKKSGLINDIIRMSLTDDEVRESVASITIELLRADVISWEEVFVALKDSGLAIDVVRFSLTDEETRNGLGELVKEILPDLLESGAIDPKDLVSGPSDKHGNKNHGKFKDRDDDFDNHKKGHKRDDNLPIEGAVPISHPIAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3APR6</accession>
    <name>G3APR6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable cytosolic iron-sulfur protein assembly protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CIA1</name>
        <name evidence="5" type="ORF">SPAPADRAFT_55738</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for the maturation of extramitochondrial Fe/S proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat CIA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375513.1">
        <property type="nucleotide sequence ID" value="XM_007375451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APR6"/>
    <dbReference type="FunCoup" id="G3APR6">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APR6"/>
    <dbReference type="GeneID" id="18871965"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55738"/>
    <dbReference type="eggNOG" id="KOG0645">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_8_1"/>
    <dbReference type="InParanoid" id="G3APR6"/>
    <dbReference type="OMA" id="IREIRWS"/>
    <dbReference type="OrthoDB" id="284782at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03037">
        <property type="entry name" value="ciao1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028608">
        <property type="entry name" value="CIAO1/Cia1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="7"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="51"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="121"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="168"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="343"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="361" mass="40480" checksum="4DD84E5FD66CE911" modified="2011-11-16" version="1">MQLLHSTQAHTGPIWSITTHATLPLIATASQDKTSKIYSLTSHHNLLTILEKTHTRSVRSIAFKPPLDNFDLPVLAAGSFDSTISIWGIDEPEFYADVESESDHERIICDPNNEWNLMALIEGHENEVKSVAWNHQGNLLASCSRDKTVWIWETDQETLEEFECIAVLNDHEHDVKYVCWHSTRNILASCSYDDTIRIYAEDDDDEFACVGVLNGHEGTVWSCAFENPATPTDKIRLASVSDDLTVRVWVGTVPEHSDKQLPSSIRHNKEMVWEQECILPQAHAFPIYSLSWSATTGKIASCGSDGKIVVYKEVDGTWEVEATHEASHGVSEVNCILWGKLDDKEILITGGDDGCVNYWQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ51</accession>
    <name>G3AQ51_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein RDH2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RDH2</name>
        <name evidence="4" type="ORF">SPAPADRAFT_61963</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376176.1">
        <property type="nucleotide sequence ID" value="XM_007376114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ51"/>
    <dbReference type="GeneID" id="18874125"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61963"/>
    <dbReference type="eggNOG" id="KOG1208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_6_1"/>
    <dbReference type="InParanoid" id="G3AQ51"/>
    <dbReference type="OMA" id="LEMGTNC"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF236">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="333" mass="37280" checksum="F199E5D92D8CEAF4" modified="2011-11-16" version="1">MSAPVVPARTCQQWKQLFHGFFPGKPTFKEDQYPSFEGKVVIVTGGNTGVGYETVKSLAGSTKAKLYIFSRSEEKTLEAIKQIQLEVAKEYNIPNREIHFIKVDLSDLETIKPAVQKFLSQESRLDLIIHNAGVMTPPVGSKSKQGYELQLGTNTIGPHLLQRLLDPIFIETSKKNAPGVSRIVWVASSAHQSAPLGGFHWDDLNFEKIKISKTDEMMVYGQSKAGNIMQAKTWTRKHNTGSQIISSSLCPGYLNTDLQRHVSSFEKFLANWLLHPRRMGAYTELFAAFSPDVKDGSYSISFGIPSQARDDLLQDDACDKVWNFLDEATDPYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ87</accession>
    <name>G3AQ87_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DUF300-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_154586</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376212.1">
        <property type="nucleotide sequence ID" value="XM_007376150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ87"/>
    <dbReference type="FunCoup" id="G3AQ87">
        <property type="interactions" value="587"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ87"/>
    <dbReference type="GeneID" id="18870990"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_154586"/>
    <dbReference type="eggNOG" id="KOG2641">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012923_4_3_1"/>
    <dbReference type="InParanoid" id="G3AQ87"/>
    <dbReference type="OMA" id="CLAIFWK"/>
    <dbReference type="OrthoDB" id="5348404at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044395">
        <property type="term" value="P:protein targeting to vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="508"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="508"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="536" mass="61668" checksum="F176DC4FD70F131F" modified="2011-11-16" version="1">MLEDDTGGGGSTPGNILPNWIIIVSFYCSIISAMIVILSIVLHLVNYRKPFQQRLMIRIQLIVPLFAFSCYSMLINQSSVFNKYVLEPIREVYEAFVIYTFFSLLTELLGGERNIIIMTSGRSPVRHPGVILGNCLPPMDISDSHTFLAIKRGILQYVWLKPIIIITTFLTQLLGWYNVNDLSFKSIYFWLTLIYNMSVTLSLYCLAMFWKILWNDLKPYKPVGKFLCVKLIIFASYWQGVILAILNFFQVLGDTTNEGDISIGVCIQNALLCVELIGFAWGHWVSFTYKPFTISELPYGRYQLKYSLKDCFGFRDLINDFKLTYYGDYYKNYKQFDSVDAMVAHPDSKGRMSRIHQGLRYHYDGKHKHWLPDNTNTLTVSSPIIQSTSEIHALDNSSLYSNNTSMRAIYPSSPKQSPPNSPVSAVKTMPPEQTIEEIIANDPDLSVVDYSQESFNQDEEIYQMAIKQVGNYKLDQVGIKKMLNYPIVDEVIDSHVYGYKVKKLRQRQRLRDQHRRGERTEIQGGSNESYRYGSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQE1</accession>
    <name>G3AQE1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Lon protease homolog 2, peroxisomal</fullName>
            <ecNumber evidence="9">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_140237</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">ATP-dependent serine protease that mediates the selective degradation of misfolded and unassembled polypeptides in the peroxisomal matrix. Necessary for type 2 peroxisome targeting signal (PTS2)-containing protein processing and facilitates peroxisome matrix protein import.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10 11">Belongs to the peptidase S16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="9"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376266.1">
        <property type="nucleotide sequence ID" value="XM_007376204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQE1"/>
    <dbReference type="STRING" id="619300.G3AQE1"/>
    <dbReference type="GeneID" id="18870282"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140237"/>
    <dbReference type="eggNOG" id="KOG2004">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004109_4_0_1"/>
    <dbReference type="InParanoid" id="G3AQE1"/>
    <dbReference type="OMA" id="RCMNPVI"/>
    <dbReference type="OrthoDB" id="2411602at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19500">
        <property type="entry name" value="RecA-like_Lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000021">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03121">
        <property type="entry name" value="lonp2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054594">
        <property type="entry name" value="Lon_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027501">
        <property type="entry name" value="Lonp2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008268">
        <property type="entry name" value="Peptidase_S16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="ATP DEPENDENT LON PROTEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22667">
        <property type="entry name" value="Lon_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01046">
        <property type="entry name" value="LON_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="2 9 10" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 10" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="14">
        <location>
            <begin position="13"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="13">
        <location>
            <begin position="836"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="363"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="505"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Microbody targeting signal" evidence="9">
        <location>
            <begin position="1086"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="507"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="active site" evidence="9 10">
        <location>
            <position position="942"/>
        </location>
    </feature>
    <feature type="active site" evidence="9 10">
        <location>
            <position position="985"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="579"/>
            <end position="586"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00050665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW31488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1088" mass="122148" checksum="FB7FAE190D458A8A" modified="2011-11-16" version="1">MSYRKSTKKQLVLPTYKLDSNLVLLPGIMYNVTFSRFKAAALLYRFKDLVSKVSIINNLLNEYDFDTAEGESPTMISRDAIEGITDFFKGEHKQEDVDEFEWLTLAIIPNTDKIKSPTKIDNNVYTVVRIVGIVDDTTNIKLTLQALARGVVVPKDTKTNESLVEVDWNTSVYDLSKLVKSLKGNASVLFKSIDKFLSEYRQALNNGTTTQKLSLTKSDKDKNSPITDKQNDLLTLNPLANALYLQLAGSKDFSKAFISLQKLYGTEIDSKTYLRLIDLTCAILPFPNYERLNLLNKHKLGERAIEINNMIARLTKVFDELRANNSFVNHWFHNEATNIQKANVVSNELKSIRILLEGMTKSNKENGRKANSPAVPKRHPDEDFDEEEDDDELKAITNFIKNKLPHITTIAPDSKRLLVKDFKRIKAGNTPGGNSDFHVIRNYLEIVMDIPWDKYVSKFKSNRDIDIKAAKKQLDDDHYGLEHVKKRLIQYLVVLKLLGMNTKQSPAAPPATPALPPTSSNSNESMLQGNIVIANNDETYHVRQQAKSQVKQSMTESRLDQESLEVSKHNKSPIIMLAGPPGTGKTSLAKSIATSLGRNFQRISLGGIKDESEIRGHRRTYVGAMPGLIIQALRKARSMNPVILLDEIDKVIGGNSGLNRFNGDPSAALLEVLDPEQNNTFIDHYLGFPVDLSQVIFICTANEPHNMTKPLLDRLEMIEIGAYDYNEKYIIGKKYLLPRQVKRNGFPVKESMEEFVNISETTMKKIIIDYTRESGVRNFERKLGTICRFKAVEYCECLNSDTTYNPVVDEQDLAIYLGVPYNMPNYSSAEGTTIGNSRVGVVNGLSYNSDGSGSVLVFESIGFDRRVGGGSSSSGSGATLNMTGRLGEVLLESGKIGLVFIKSMIYKNLLKGQDQKTLLDKFNNLDIHIHVPMGSISKDGPSAGITMALSFLSVLLNKPVPSEFAMTGEITLRGLVLPIGGVKEKLMGAHLNPSVTKMIVPRANRKDLIEEYSRSIEEVGEVIDQNLINDLLKDNEEHEFKMNQVEKFYLNKYGIQLFYAHEFYDVMKIVWNDSDLLKQATRVVEYHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQK4</accession>
    <name>G3AQK4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 5">Branchpoint-bridging protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_62151</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Necessary for the splicing of pre-mRNA. Has a role in the recognition of the branch site (5'-UACUAAC-3'), the pyrimidine tract and the 3'-splice site at the 3'-end of introns.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BBP/SF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376329.1">
        <property type="nucleotide sequence ID" value="XM_007376267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQK4"/>
    <dbReference type="STRING" id="619300.G3AQK4"/>
    <dbReference type="GeneID" id="18874199"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62151"/>
    <dbReference type="eggNOG" id="KOG0119">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016864_1_1_1"/>
    <dbReference type="InParanoid" id="G3AQK4"/>
    <dbReference type="OrthoDB" id="6777263at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045131">
        <property type="term" value="F:pre-mRNA branch point binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF45">
        <property type="entry name" value="SPLICING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="7">
        <location>
            <begin position="142"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="168"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="266"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00017984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="307" mass="33204" checksum="B8FCAEC714B2832E" modified="2011-11-16" version="1" fragment="single">DYPEINFVGFLIGPRGKTLRRLQDESGARLQIRGKGSVKEGKSAKAIDDKSMASMNGADSAEDDLHVLITSDSQQKIAKAVQLTNEVIEKLIFSPEGQNELKREQLKELAVLNGTLRETKPFDPEAYQKRQRKTMDISQIICKICGNIGHFARDCKQNNANGMKRQFDTYEPRQNNPPPPSGTSYEEQPWKKSRPDILPPWQTANTAPHSLPPPPPSLIPVQSGTYPPPSLPVQSGTYPPPPPSSQAPPPGPPAPPPGIDTKPINHLPPPPPPTKTKPPPPPPGSKPPPPPPSAKSKPPPPPPPSGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQK9</accession>
    <name>G3AQK9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="17">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">SPAPADRAFT_72340</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 17">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="17"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376334.1">
        <property type="nucleotide sequence ID" value="XM_007376272.1"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQK9"/>
    <dbReference type="GeneID" id="18875427"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72340"/>
    <dbReference type="eggNOG" id="KOG0206">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000846_5_2_1"/>
    <dbReference type="InParanoid" id="G3AQK9"/>
    <dbReference type="OMA" id="FNKHKFF"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140346">
        <property type="term" value="F:phosphatidylserine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000014">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF180">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE DNF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 16 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 17" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="464"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="508"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1102"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1133"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1191"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1222"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1249"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1290"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="19">
        <location>
            <begin position="80"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="20">
        <location>
            <begin position="1070"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="244"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="14">
        <location>
            <position position="580"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="580"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="580"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="581"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="582"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="582"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="713"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="754"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="777"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="813"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="894"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="895"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="896"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="1018"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="1024"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1044"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="1047"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="1048"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1048"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EGW31556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1477" mass="167991" checksum="D51E7844F6142AF4" modified="2011-11-16" version="1">MEELKPEQSDPINPDRVSATKGNSYNSETTSKSTVSSAVNGLINNRPEYLQEIYDNIPRTIYFNYDLPDDMKDPSGHPIISYPRNKIRTTKYTPLSFLPKNIIFQFTNIANFYFLVLVILGAFQIFGVASPGLAAVPLIVIVCITAFKDALEDYRRGTSDSELNNSPIHLLSGTHNPNVLTTEVGPWRRFKKACTRGTFATFRAFKKCGVFICGNKKKKQEYIRQQVQEEEYALHRVSTVHSEYSYQSQPEPRLSLDSRRSMQSGRSRHRPTVALPNSLLNPVLKKENENVGDDKRPSFKNRRWKDVNVGDIIRIRANEEVPADVIILSTSDPEGNCFVETKNLDGETNLKTRNVLKCGGTNNLKHSDDLGDTKFWVECDAPNPSLYTFRGTIHYENYDGSGNLVNPDEKEAITPDNVLLRGCMLRNTKWVIGLVVYTGPESKIMLNSGITPTKKSRISKELNLSVIINFLLLFILCFISGLINGLFYRRTDNSRAYFDFQPFGGTPAINGVLAFFVTLIIYQALVPISLYISVEIIKTLQAFFIYSDLKMYYEKLDFPCIPKSWNISDDLGQIEYIFSDKTGTLTQNVMEFKKCSIGGKSYGLAYTEAKQGLDKRNGLDTVAEMNKWKKRISDDKQEMVELLTKYSSNDQLREENVTFVSSEYVKDTMMEDSSRKEINERFMTALALCHTVVTEVSETDPGYRNFKAESPDESALVSVARDLGIVFKERLRKSVIIDIYGEELTYELLDIIPFTSARKRMSCILRAPDGRVIVYTKGADNVIFQRLDPHNNSNDVISKTALHLEDYATEGLRTLCITEKEVDYDYYKAWSARYGEANACIDDNRDELISKVEDEIESNLILLGGTAIEDRLQEGVPSSIAILAQAGIKLWVLTGDRIETAINIGFSCNLLENEMKLLVVRPEENDLENVAYVDALITGYLKDHFGIDTSDPASIPPLVEAAQKDHSAPNPNFAVIIDGAALHLVFQDLVELEDESVKALKDKFLLLGKQCKSVICCRVSPSQKAEVVKLVKDSLQVMTLAIGDGANDVAMIQAANVGVGIAGEEGRQAVMSSDYALGQFRYLTRLLLVHGRWSYKRLAEMVPCFFYKNVVFTLTCFWYGIFNNFDGSYLYEYTYLIFYNLAFTSLPVIVLAVLDQDVSDTISLLVPQLYRSGILGLEWSQYKFAWYMLDGLYQSVISFFFPYLLYLVSFQNPQGTTIDHRFWIGVVAISISVTACNIYVLLQQRRWDWLTLLIDGISILLVYFWTGVWSANVTAAGEFFRAGAQTLGTLAVWCCIFIGVLVCVLPRFIYDFLFRNFKPKDIDIIRERARMGEYDDYPEGYDPTDAEDVERHRLLTEIIDHDPKMLEQLQNEEKQAQEKPEELDHNPLARTFNTIKKRATIQRSRRNTVRSRKNTINDQFHRPIDINLLRQQMIQRGEYQTARTSLERINTTHELPGLTQAETLIQYHTRNSMTLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQP1</accession>
    <name>G3AQP1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_141135</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the RBR family. RNF14 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="3"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376366.1">
        <property type="nucleotide sequence ID" value="XM_007376304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQP1"/>
    <dbReference type="FunCoup" id="G3AQP1">
        <property type="interactions" value="169"/>
    </dbReference>
    <dbReference type="GeneID" id="18870349"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141135"/>
    <dbReference type="eggNOG" id="KOG1814">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021364_2_2_1"/>
    <dbReference type="InParanoid" id="G3AQP1"/>
    <dbReference type="OMA" id="PRSWCQG"/>
    <dbReference type="OrthoDB" id="1431934at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20341">
        <property type="entry name" value="BRcat_RBR_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20354">
        <property type="entry name" value="Rcat_RBR_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23820">
        <property type="entry name" value="RWD_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047548">
        <property type="entry name" value="Rcat_RBR_RNF14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RWD" evidence="13">
        <location>
            <begin position="13"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="165"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="169"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW31588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="481" mass="55822" checksum="149268CF6166B454" modified="2011-11-16" version="1">MDEPTFSDDIRIQELESCQTIYPNLRISKLSGSIDIPIKLDTESEIRLIQNSTVLASKQVVNLPAITLDFTLPEGYPYDHPPQIKLSSQIIHPDKLNKLETELSSVWFDYKDQVIFMLIDKLLDTTQNNIDEILPSPIEIIDHYQLYYDIIDYDQSTKQAEFNNSTFACEICQNNYSGLQCSKFDCGHVFCNDCLYEYFSSVITTGEIDKVHCPDFECTKLHIKTRDSFAKLETWMENDSKVKEMVTTMLTPTVPLALLTHIFKPKTDQANELVKRYMTLYKKSQFEFIGKLLPNRLVSCPRIGCDEVIFREDLHERLVRCPKCQYSFCNDCRMSYHTRFKLCNKVNENENYSGIPVADLEAYPLYPSDSYERKTLNARYGRITILRAIEEYKMDKLFEAMLKEGNQARECPGCKTVIEKSEGCNRMKCSECSTFFCFNCGNQISRNYDHYSDTTSPCYRLLFFGMLGAEQDDDDDDEFID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQP4</accession>
    <name>G3AQP4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">UvrD-like helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_51586</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376369.1">
        <property type="nucleotide sequence ID" value="XM_007376307.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AQP4">
        <property type="interactions" value="325"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQP4"/>
    <dbReference type="GeneID" id="18871572"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51586"/>
    <dbReference type="eggNOG" id="KOG1801">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000459_2_0_1"/>
    <dbReference type="InParanoid" id="G3AQP4"/>
    <dbReference type="OMA" id="PWHQSEL"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035649">
        <property type="term" value="C:Nrd1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033678">
        <property type="term" value="F:5'-3' DNA/RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001147">
        <property type="term" value="F:transcription termination site sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045005">
        <property type="term" value="P:DNA-templated DNA replication maintenance of fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060257">
        <property type="term" value="P:negative regulation of flocculation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031126">
        <property type="term" value="P:sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016180">
        <property type="term" value="P:snRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd21408">
        <property type="entry name" value="1B_Sen1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18042">
        <property type="entry name" value="DEXXQc_SETX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000326">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001152">
        <property type="entry name" value="tRNA-splicing endonuclease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044340">
        <property type="entry name" value="Helicase_Sen1_1B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024481">
        <property type="entry name" value="Helicase_Sen1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056474">
        <property type="entry name" value="SEN1_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF495">
        <property type="entry name" value="HELICASE SENATAXIN ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23576">
        <property type="entry name" value="SEN1_barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12726">
        <property type="entry name" value="SEN1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="11">
        <location>
            <begin position="1266"/>
            <end position="1575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="878"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="937"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1785"/>
            <end position="1825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1867"/>
            <end position="1937"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1427"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1801"/>
            <end position="1811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1812"/>
            <end position="1822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1869"/>
            <end position="1897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1928"/>
            <end position="1937"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="1287"/>
            <end position="1294"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1937" mass="220335" checksum="147EA2B8CFD4D77B" modified="2011-11-16" version="1">MEKPEGFDELVANIDKSHKEPGNSSIQELVLKQAVTYLLRNKNRQHWFCDEYMYPITVHSLVLFSFPNNPTLCQLQPYIANSLSQCRECMKSYNRGKADLRLNFAINKGVKLDKVNQFLNTIIQWEVTVVGKKLEDMENTMSDISTGYDVNTLYWCLYNPNILRFDSELRQKFDRIVRYLHTQKSLPLPDELLPGTIYMAFEGSLEEKYWVEQWLNKLHHDNFRFTKATLQEPVVYEFSIHLYQIQNAKFYNDRNAIKFWNNFYRFVDFIDDESFILRFNTPRDIRVMSEFTELRLYPVIRLLMNCLLSYLKEPLPVLLKCFNKLLSRLLTKFWTSAMPIQSIQVLDAILSNPEFTKQLQTASIVESDVSDYNLSEISSWMFSILPSLGQLPRQSTCIRMGTFLLNQSISDVGSKDIRMRKVMALRQLGCRIFYSELQTLDSEFREQNFSLRLMTVRDVRNAVDNQADIIVDLALDSEDSTLAVKLVVRCMQYDLSILAHNTCMFAQGVVPTIFDTFPILWDSLTKRKIYSNTLLVHELIKVLTIGINCTLFAPTKNPDPNKQLADCKTQHNDNMKKMTKLVNQLLEKLSLADPSTLKQLINEPECSIAIWSCVFNPQLSQSALDLLNEAFDAGGRLETIQVILSKDLKVTLHAITTNLNVLTQMGLYEPSPKAIRILMDVINALADPLNPILASAPKDCVDEVLQFWKSIWQFLIMVYKRTFIWANQFHLDELVEFTRDTLDLSHLILASYRILLDNVKSETVDVSHTLFIDFMNAFDSVIPWLRLGDLSLLNSCVSLVFKGFELASERNFPIDAGFIETFAKFGAKAKKFNNKLSEVQRQQILSKAREFNPEIVDRVVDEATKHRAKRNIEIIDVSEPSSSPEPAAYRYQSRSVTPKQQTLSRFGVVTQEAPIAPPPSKDYKPSNLEAIRKELKSARASPLPKSDVAPAPPRPAGFNSKKVPPVVGRSLNALKKKKHDSDSSDDELETDVSDLFIDKVKKKPKIVEVDMNLNPVHRSLGKKMVDPVKLEQERMRLRLNVNLKPLYNTILRWRYNTNSDYPSEDKSSYTQVKDTYDNVKDYVSTTEPLLMLECWQGIQQAKATGVEEPFEMLIGTRTSVDGFFDVFASVEKKTIQDRKIGDSDLLVLGIHDGVTKSPREIMNHLKSESATTCLAKVREIKYNNTDYCDITFRVYPSGSMVGVLLPKSIIVGMRVMQMITVEREFSSLRGLQYYDLLFPILQARPNDPIPIHDSATEDMCKKFNLNVSQAKAILGTHQSEGFSLIQGPPGTGKTKTILGIVGYSLSQKDEKILDIPGHTPTTDPAKILICAPSNAAVDELVLRLRDGVKNSSGETMNLKVVRLGRSDAINASVRDLTLEELVDKELQTKAVDVNIDPTIRQQHSKCIEERDALRARLVNESLSEKEMTDLEDRLRTVNKKRSELAKRLDEQRERASIAYRTKEIERRNAQARILSQAQVICSTLSGSAHDFLASLSLKFDKVIVDEACQCVELSAIIPLRYGCRTCIMVGDPNQLPPTVLSQAAASYNYEQSLFVRMQKNHPDSVYLLDVQYRMHPQISQFPSAEFYNSKLKDGEGMLEKNDRPWHKDPPLTPYRFFDIVSKHERDDQSRSLFNVEEARVALELVQKLMTILPQDKFRGRIGIISPYKEQIRSIKNEFIRRYGRAIQDDIDFNTVDGFQGQEKEIIIMSCVRASPSGNVGFLSDVRRMNVALTRARTTLWILGNKDSLSRNNVWRRLLEDASNRDCISKAYPGFLNMSGVKRQHIEPGNSIHDNGSDSIQPKKKKKNKHKQTPPPPPPPSVPKIPEVSTVHAIRSGIFDGPKVPKHVKTSKTDLIAAAHKINTEKAHKTNTPTPTQSSYLKKTNPNGLPAIPTNSGFINRGPSADTKPTSSGTIKPPPKKTNSGIFIQRKKPPHKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQP9</accession>
    <name>G3AQP9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DUF221-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_72374</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376372.1">
        <property type="nucleotide sequence ID" value="XM_007376310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQP9"/>
    <dbReference type="FunCoup" id="G3AQP9">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="GeneID" id="18875430"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72374"/>
    <dbReference type="eggNOG" id="KOG1134">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002458_0_2_1"/>
    <dbReference type="InParanoid" id="G3AQP9"/>
    <dbReference type="OMA" id="CSCKKEN"/>
    <dbReference type="OrthoDB" id="1689567at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF5">
        <property type="entry name" value="RE44586P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="467"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="514"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="562"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="601"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="649"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="676"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="717"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="741"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="10">
        <location>
            <begin position="28"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="11">
        <location>
            <begin position="224"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="9">
        <location>
            <begin position="467"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="311"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="849" mass="96725" checksum="5FB62E0291002D00" modified="2011-11-16" version="1">MLEEYSVADVLLSDGRYRPPSTRVARYQIIIASTLGLFALMVFSLLRIKYPKIYVANLNHTNFNYLHSSSRRHLPKIPAKSLFGWIPIVYKINESQVLEHAGLDAVVFLGFFKMCIKILTVCLIFAIVVISPIRYKFTGRLDQDYPDDDDDNNGTTIKMIKRIILLGTEIPTEGEGATYKAYLWTYTIFTYVFTLVTAYFLFSQTNKIINMRQKYLGGQNSITDRTVKVSGIPAFLRDEVTLTRHIEGLGIGEIDSVLIVREWQNLNKLCKLRNRVLRKLEQYWIEYFHQNGIMNKSQILSASFQPQPTATLGLENGYRDEEGGEDATPTSSNEEDRQSQASTLMDQISEIIEDANHPSLGLVDEAVSRPKINKGLFGIFGAKVDAINYYAEQLEVIDKEIVRARTREYPATSTAFLTMKSVAQAQMLAQAVLDPKVNHLITSLAPAPHDIRWDNLCLTRKERNTRIFTVTMAIGLVSILMIYPVRFLASFLNIKSISKIWPSLGNALRKSKWATTLITGLLPTYVFTIFNIIIPFFYVWISGKQGFTSHSDEELASVAKNFFYIFVNLFLVFTTFGTASLSDTTKIAYELAQSLRDLSLFYVDFIILQGLGIFPFKLLLLGNLIRFPIESLFWCKTPRDYLALYKPPVFNFGLQLPQPILIFIITLVYSVMSSKILSAGLIYFIIGYFVSKYQLLYACVHPPHSTGKVWPLAFRRIILGLLIFQLTMAGALALQEAFTCATFLAPLPILTLYFLWNFQYQYIPLSMFIALRAIENQEVSPDNDLENNGGDNNNKTLDERRELNTKYEYPNLVRDLDGPLIAIDGQDVLTINKDGQTVRKFQDFSEWYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQV9</accession>
    <name>G3AQV9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Exocyst complex component Sec6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_67273</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC6 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375964.1">
        <property type="nucleotide sequence ID" value="XM_007375902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQV9"/>
    <dbReference type="FunCoup" id="G3AQV9">
        <property type="interactions" value="268"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQV9"/>
    <dbReference type="GeneID" id="18875152"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67273"/>
    <dbReference type="eggNOG" id="KOG2286">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011776_2_0_1"/>
    <dbReference type="InParanoid" id="G3AQV9"/>
    <dbReference type="OMA" id="LAYDSHY"/>
    <dbReference type="OrthoDB" id="190098at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051601">
        <property type="term" value="P:exocyst localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.70">
        <property type="entry name" value="Exocyst complex component Sec6, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010326">
        <property type="entry name" value="EXOC3/Sec6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042532">
        <property type="entry name" value="EXOC3/Sec6_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06046">
        <property type="entry name" value="Sec6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="825" mass="96293" checksum="084E06F7EB7140E1" modified="2011-11-16" version="1">MSDSALSKIGELIKMEDDLVKISSLRQQFLKEKSSLDINLSATTQRQIDAIMDNLSKLNNTSKRLQSIKGNMNKINQIYDESITDIKDYDTIRKMTIVNSFLTQVSNLKGDIFDFKAFLDILNQNISDEYDQLRQSLTYPLPNIFQIHYAYTQARNFTDYMETYSKSLSDDSRSLVLKIVSPMKHTRVLFDELLKEAIISLIEMAKDENYECIFNVVKVIEIEAREDMKVAMMESLKLSHDNSMINYSNFRSSRRNYKKFFYDKLEESLDETFNNCIEYYPEKMQVYDNLGWLEEELIFVKKSLIMFFPPTWKIDEFIQNVYFNRLHKFTMEVIESEPTAEDLMKILSYDKRYGKFIESLQEGKRTPKDQRSIIGEDLKNVVLEDYLKVIVSKMGEWNRNLMKQETESFRQRSEQPTTYNYFQSYLDDEQGMAVERQFEADVFVLPDFTTPLTMLQQQADVAAESGYSNILVGVIENWSKCYLERIENFRNILEDEIDRYMSIYNKEKYLIKESAVKRLFKRKPSQAIIDPDALTDEEINEISRPGLIEYLTALGNTFVINTDRLQDKFLPKYKDNVHAHYQDRIQQAIESTLTPSTDLNAQIITAIVEIIINDLYPALSTLFTKKWYEEDQQTSNDPISAQLIVQTIKEYMQDLRSYASYDMYSLTFNVLLDKFISSYIRIGLENILHGDGKKIDPTAVKKYKSFSEAVDRDIAVFFGGLSDLFTAKDGYYLMTSLRALEFVADIGMCPDPMNDIPHIWEKNILPTFYFCKVDVVRATCLCRKDMDKVQINQLIPELEAIQRGYHANVEPPEVIVTALANIEFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR54</accession>
    <name>G3AR54_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61806</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376007.1">
        <property type="nucleotide sequence ID" value="XM_007375945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR54"/>
    <dbReference type="GeneID" id="18874060"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61806"/>
    <dbReference type="eggNOG" id="ENOG502SYY5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1031205_0_0_1"/>
    <dbReference type="InParanoid" id="G3AR54"/>
    <dbReference type="OMA" id="NYDTHME"/>
    <dbReference type="OrthoDB" id="3997968at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="270" mass="30071" checksum="5FED4726EC04049D" modified="2011-11-16" version="1">MSYVPQTPERPSRISRHCTTSTPISTTHNLLTPCTATKKPFLNVAPITTPLAHTNKHQIPVTPEFTPQKSPARNTTGSGTTRRKKINDLLFETDAELGKERLSFGLLLPVPSTVGSGRKRRTSGVSTTTSSGNITFSQPLCMHEEEEAIEDVTVTATPKAQVITSSMVDNWHGKSFNNAFSDEEDDDEKEVETMTLGQSKLIPRVKLENPFIDGPTTPKTKKEDNPFIEDSSKVDYETHMELVNHRTGVKRIVKLNKNQMKIKPKKLSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR86</accession>
    <name>G3AR86_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Leucine-rich repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_67338</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376039.1">
        <property type="nucleotide sequence ID" value="XM_007375977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR86"/>
    <dbReference type="STRING" id="619300.G3AR86"/>
    <dbReference type="GeneID" id="18875155"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67338"/>
    <dbReference type="eggNOG" id="ENOG502SF6T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029128_0_0_1"/>
    <dbReference type="InParanoid" id="G3AR86"/>
    <dbReference type="OrthoDB" id="4073735at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454:SF56">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="523" mass="59704" checksum="D9D982AEF4B1D1D7" modified="2011-11-16" version="1">MPADNNDWVMKFLTFYLSRLPDHAQIFIFDLLDPEYIKDFFLYFPDNKLHQLLVDHYFSKVLHFQMGEIVAHHHEFMYDYTSSEVVSFYGMKNILRFMKDFPKIVPRRLLLIGESDGGILKDDRISKIDELEVIIDGSDIDPDYFDYIISFPNLTRLHIMDEFIGEGMLDSRKFTKHPKLQDVAIISFEADFSDIKLPKSLRKLGVTSCSFDISDVKIPKSVSHICMSKCSIDDFAELATILPKSLSSLCLCDNELEGIVLDDLPAGLRSLDLASNEVSIELDNSNKTWPIDLCELNVSGSTIDDLFLEQLDSSIGWPKKLTKLDVSCSQINQIGKLQLPESLEILNISSNPLSWDCIKETPFRFPGNLTVLDMTECGISNLNCLKLPQSLKKLRLGNNGIDDILSYDDWSELIHLHTLDLSQNKIISLNNWKSPPKLIKLVLSNNEISELSSYCDWKALVNLRTLDLASNKITSLDNWLKPPNLVRLDLSRNPSDELSLKFPMFKEKIDAPYRSFTLASIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARD0</accession>
    <name>G3ARD0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein bL33m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_62335</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL33 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376515.1">
        <property type="nucleotide sequence ID" value="XM_007376453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARD0"/>
    <dbReference type="FunCoup" id="G3ARD0">
        <property type="interactions" value="256"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARD0"/>
    <dbReference type="GeneID" id="18874276"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62335"/>
    <dbReference type="eggNOG" id="KOG3505">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_190949_1_2_1"/>
    <dbReference type="InParanoid" id="G3ARD0"/>
    <dbReference type="OMA" id="TQVRYDP"/>
    <dbReference type="OrthoDB" id="275534at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.120">
        <property type="entry name" value="Ribosomal protein L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052008">
        <property type="entry name" value="Mitoribosomal_protein_bL33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001705">
        <property type="entry name" value="Ribosomal_bL33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038584">
        <property type="entry name" value="Ribosomal_bL33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01023">
        <property type="entry name" value="rpmG_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47037">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L33, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47037:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL33M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="70" mass="8245" checksum="10D714A4FD31514B" modified="2011-11-16" version="1">MAKKSIRTMVKLISSAKTGYEKWFNIKRSSPDLNLILYDPRAQRHCLFTEAKKRKIAHVTPKDFTRSHRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARD3</accession>
    <name>G3ARD3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62337</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376518.1">
        <property type="nucleotide sequence ID" value="XM_007376456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARD3"/>
    <dbReference type="STRING" id="619300.G3ARD3"/>
    <dbReference type="GeneID" id="18874278"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62337"/>
    <dbReference type="eggNOG" id="ENOG502RZDT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1165700_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARD3"/>
    <dbReference type="OMA" id="TRDITEC"/>
    <dbReference type="OrthoDB" id="4085867at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014841">
        <property type="entry name" value="Rad33"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08730">
        <property type="entry name" value="Rad33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="223" mass="25735" checksum="BD87AB400EB41A71" modified="2011-11-16" version="1">MPPRKTKSNLDIFEKINADTEDEILDEYSELLGDDDDLHLKELPELLQNLRIPAIFTDDIVESVEYYYNFIRNRDVSIDPSNIKQSMSFQLIKAYTITNAIRAPSDIIDIIDIDKLLFNVNRLVKFRNNYKHIRESWRLFVSDSSNIEHYKLALPDLKQVKSNLNLDNDPETRKPISESFLIDMLGCCSHDSNGNLLNYMTKEGACVTIKDFAEILGNLGELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARM5</accession>
    <name>G3ARM5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62372</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376556.1">
        <property type="nucleotide sequence ID" value="XM_007376494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARM5"/>
    <dbReference type="GeneID" id="18874295"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62372"/>
    <dbReference type="eggNOG" id="ENOG502TI9N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164159_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARM5"/>
    <dbReference type="OMA" id="MKAPQNG"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="98" mass="10298" checksum="00B1BB7AA45C2813" modified="2011-11-16" version="1" fragment="single">MGNEASKHKHKSVNKLGAAPVPAPVTSASKQVSQATAKPTPTRSSKSQLVSEARPVGTSGSGSSVAAKEASARAAEERFKKLQEQKQGQKERIRTKAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS31</accession>
    <name>G3AS31_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fructose-1,6-bisphosphatase</fullName>
            <ecNumber evidence="5">3.1.3.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">D-fructose-1,6-bisphosphate 1-phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_62487</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the FBPase class 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="5"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376658.1">
        <property type="nucleotide sequence ID" value="XM_007376596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS31"/>
    <dbReference type="FunCoup" id="G3AS31">
        <property type="interactions" value="593"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AS31"/>
    <dbReference type="GeneID" id="18874344"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62487"/>
    <dbReference type="eggNOG" id="KOG1458">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039977_1_0_1"/>
    <dbReference type="InParanoid" id="G3AS31"/>
    <dbReference type="OMA" id="YIPENCP"/>
    <dbReference type="OrthoDB" id="10256725at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072593">
        <property type="term" value="P:reactive oxygen species metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005986">
        <property type="term" value="P:sucrose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00354">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000002">
        <property type="entry name" value="Fructose-1,6-bisphosphatase class 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000001">
        <property type="entry name" value="Fructose-1,6-bisphosphatase class 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01855">
        <property type="entry name" value="FBPase_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044015">
        <property type="entry name" value="FBPase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000146">
        <property type="entry name" value="FBPase_class-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033391">
        <property type="entry name" value="FBPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028343">
        <property type="entry name" value="FBPtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020548">
        <property type="entry name" value="Fructose_bisphosphatase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006778">
        <property type="entry name" value="PRK09293.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11556">
        <property type="entry name" value="FRUCTOSE-1,6-BISPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11556:SF1">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00316">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18913">
        <property type="entry name" value="FBPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500210">
        <property type="entry name" value="FBPtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000904">
        <property type="entry name" value="FBPtase_SBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00115">
        <property type="entry name" value="F16BPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00124">
        <property type="entry name" value="FBPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fructose-1-6-bisphosphatase class I N-terminal" evidence="14">
        <location>
            <begin position="21"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Fructose-1-6-bisphosphatase class 1 C-terminal" evidence="15">
        <location>
            <begin position="212"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF18913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW31880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="343" mass="37719" checksum="FE2EEC54DEB22544" modified="2011-11-16" version="1">MTSAQIADSVAKQMNVETDIITLTRFILEEQQHVAPSATGELSLLLNSLQFAFKFIAHNIRRAELVNLIGVSGTANATGDTQKKLDVIGDEIFINAMRSSNNVKVLVSEEQEDLIIFEGGGKYAVCTDPIDGSSNIDAGVSVGTIFGIYRLKEGSKGSINDVLRKGTEMVCAGYTMYGASAHLMLTTGYGVNGFTLDTQLGEFILTQPNLKIPNTRSIYSVNEGNSFYWPDYVKKYIEDLKKPQDNGKPYSARYVGSMVADIHRTLLYGGIFSYPADTKSKNGKLRILYECFPMAMLMEQAGGRAINDKGERILDMIPKGIHDKSGIWLGSKGEIEKFVTYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS86</accession>
    <name>G3AS86_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF38 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_52227</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377078.1">
        <property type="nucleotide sequence ID" value="XM_007377016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS86"/>
    <dbReference type="GeneID" id="18871647"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52227"/>
    <dbReference type="HOGENOM" id="CLU_1230568_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS86"/>
    <dbReference type="OrthoDB" id="2021138at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="26216" checksum="5716CA30BA8EF4B7" modified="2011-11-16" version="1">MAPGSISNISKLKALKCVEISGKKISRVITELENYGVRDLRINELAIRIGEYDINGLQRLEECFNLESLDTFQFETKYCKLPDTSERDLKGFFSRLVNVKNLYLSPEILAFANIMGEFKPNTLEMFAFKQTSHDLDSIYHELEKHLRNQEASIVAIRTGGYHPLLYFKDAFECVFEVHWKVDVDLIRYMKENSSRYPKLKHLIIGNNYCFIERYGKEVVNIKRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASA8</accession>
    <name>G3ASA8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">D-threo-aldose 1-dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_62512</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376681.1">
        <property type="nucleotide sequence ID" value="XM_007376619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASA8"/>
    <dbReference type="FunCoup" id="G3ASA8">
        <property type="interactions" value="201"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASA8"/>
    <dbReference type="GeneID" id="18874355"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62512"/>
    <dbReference type="eggNOG" id="KOG1576">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_7_2_1"/>
    <dbReference type="InParanoid" id="G3ASA8"/>
    <dbReference type="OMA" id="FPRSSYK"/>
    <dbReference type="OrthoDB" id="5286008at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045290">
        <property type="term" value="F:D-arabinose 1-dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070485">
        <property type="term" value="P:dehydro-D-arabinono-1,4-lactone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686:SF1">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="21"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="350" mass="39663" checksum="C4CBA5DC3B07EA7F" modified="2011-11-16" version="1">MTKAIGKSEVVEPYSIKNIPPLIIGGAVFNTQYSSDPHSLPIQEIIQTAFDLGINALDTSPYYGPSEELIGNALTKITYPRESYYICTKVGRVKLNDFDYSRSSVRKSIERSLTRLNTTYLDLVYMHDIEFVEENEIIEALKELKLLKVEGKVKNIGISGYPIKFLYKIALLAKSNPEIGPLDGILSYCNGCIQNIKLFEYYDRLINECGIQKVMNGSILSMSLLRSDITHSFHPASQELKDKVYDISQQLLKEDNVELAELATRFALREWLFETTKQTSSELTWNESTAIVLGVSNIKELKVAIDSYWNIKENVHNINTTDIPLVKKVQDLLGKQHFNEVWPSGIPGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASD9</accession>
    <name>G3ASD9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Manganese/iron superoxide dismutase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_142825</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377090.1">
        <property type="nucleotide sequence ID" value="XM_007377028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASD9"/>
    <dbReference type="FunCoup" id="G3ASD9">
        <property type="interactions" value="146"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASD9"/>
    <dbReference type="GeneID" id="18870489"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142825"/>
    <dbReference type="eggNOG" id="KOG0876">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057349_1_1_1"/>
    <dbReference type="InParanoid" id="G3ASD9"/>
    <dbReference type="OMA" id="MTAREPN"/>
    <dbReference type="OrthoDB" id="275227at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S26, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="2">
        <location>
            <begin position="125"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="2">
        <location>
            <begin position="210"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="259" mass="30185" checksum="4088FBAF8F89EEAC" modified="2011-11-16" version="1">MFSRVIAKRAAGVRLSTRSLHRVPKLEQHHQWSQEGISGVYSPEQFKMAWTDYQHYLLTNLTLLTNGTAYENKHPYQIILQSAKNTTDQHIFHYASQAHNNHLFFQQLTSKDQAQETRPSKFLMQRLVQQDIADVATLRSKIVELGSSTFGQGWLFLVEYPDKQIKLMRTNNDGTPYYYGKSQVLDLNGGVDETSYKFVENLKQRAANGEREYTLPLLSINLWDHAYIRDYGVNGREKYLENVFDCIDWNVINKRLFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASP3</accession>
    <name>G3ASP3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_62588</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376762.1">
        <property type="nucleotide sequence ID" value="XM_007376700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASP3"/>
    <dbReference type="FunCoup" id="G3ASP3">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASP3"/>
    <dbReference type="GeneID" id="18874390"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62588"/>
    <dbReference type="eggNOG" id="KOG2214">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009031_5_0_1"/>
    <dbReference type="InParanoid" id="G3ASP3"/>
    <dbReference type="OMA" id="SIVPWPH"/>
    <dbReference type="OrthoDB" id="10049244at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071618">
        <property type="term" value="F:lysophosphatidylethanolamine acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007114">
        <property type="term" value="P:cell budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006642">
        <property type="term" value="P:triglyceride mobilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07229">
        <property type="entry name" value="Pat_TGL3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF44">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="5">
        <location>
            <begin position="56"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="453" mass="52137" checksum="FB4A1C0FBAB97A51" modified="2011-11-16" version="1">MGTKLKIEEYINEVLSCLDYLNETLNSDTDEFIMNSRQLKLDFFHDARQSFGCTALVLQGGSLFGLCHLGVVKALYLKGLLPRIIGGSAVGAAVASLVCTLTDKELVPILMSIEELMKNIDILNHDVDEIYGNVIENVVKKGYSQDILIFMKFVRDTIGDVTFEEAYMKTEKILNIVVHPTNQAVPSLLNYVTAPNVIIWTAIYASIGTGVLSDTVQLYVKDYNNEISLQNPDLDVQFLKPQDVTYNQQFFQRNDSETTIPKQSPYTRLTELFNVNHFIISLARPYLAPLINNDLKHYDQFGKVKYDRTTTTRTYEDLGGNRAEVKKYTKDTGYNFTKHIKNIIGMEIQHRLNVLNRLGLLNDTMKRMFIDEKPSQLQSLASIREITIVPELRYLMRDFGRVFDVHKTMENIPYWVLVGERSVWPLFPLLWTRCSIEFALDDLYNLHRNMKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASP5</accession>
    <name>G3ASP5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">[PSI+] induction protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_62589</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376764.1">
        <property type="nucleotide sequence ID" value="XM_007376702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASP5"/>
    <dbReference type="STRING" id="619300.G3ASP5"/>
    <dbReference type="GeneID" id="18874391"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62589"/>
    <dbReference type="eggNOG" id="ENOG502S5JV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1587513_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASP5"/>
    <dbReference type="OMA" id="EALCCCC"/>
    <dbReference type="OrthoDB" id="3980401at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037504">
        <property type="entry name" value="PSI_induc_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40018">
        <property type="entry name" value="[PSI+] INDUCTION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40018:SF1">
        <property type="entry name" value="[PSI+] INDUCTION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="130"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="168" mass="19183" checksum="173A59A8226BDE5D" modified="2011-11-16" version="1">MYLLDIDKRDVFTNAKTTAQAFRSWDTCMANRTCKIVAIVLIVLGGLLVIWVVTTIFQCLFMGAKCIDACCCCCCRNSNKNAYVEKQSDYPNPNMYPPHTQQRYAYQPQPPPNNAYFPTDNYNSNDNNYGGSNYSSSNYNRGYEPVQTNAGRDSPFDDHNKSEYRGYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASY3</accession>
    <name>G3ASY3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Survival protein SurE-like phosphatase/nucleotidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_155983</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376798.1">
        <property type="nucleotide sequence ID" value="XM_007376736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASY3"/>
    <dbReference type="FunCoup" id="G3ASY3">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASY3"/>
    <dbReference type="GeneID" id="18871037"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_155983"/>
    <dbReference type="eggNOG" id="KOG2157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007204_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASY3"/>
    <dbReference type="OMA" id="TNTCFQE"/>
    <dbReference type="OrthoDB" id="202825at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="entry name" value="Survival protein SurE-like phosphatase/nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027746">
        <property type="entry name" value="TTL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00087">
        <property type="entry name" value="surE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47551">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE PBY1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47551:SF1">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE PBY1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SurE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Survival protein SurE-like phosphatase/nucleotidase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="698" mass="79089" checksum="FC75359CC3D40704" modified="2011-11-16" version="1">MHVLLTNDDGPLNDNHCPYMKYLVDEINSSTDWDLSIVIPDQQRSWIGKAHFAGKTVSASYIYTTHSTSAPTDQINSYLGPFNRPETKYHNDPAYQEWCLINSTPAACADIGIHHLYSHTKNKPIDLVISGPNFGKNSSNLYILASGTVGAAMEAVTHGVKAIAVSYAFNNMDHDFHILKEAAKISVKLIQKLYAELQASEEIDLFSINVPLIDSLKLGSTKIHYAPILKNTWKSIYAPVAEPNEKGQLQFSWSPNFKQVYKDGMKDFAHTDSRVLLDEGISVTPLKAGFAMVDKLGEITLEDSQQETSDKSFLITIPQESYLYNPLVEPFKKLGYNITASRDVLNSQAKIFHYGEYEDIDLDRIHDSNYILPSYIYRKALIRKHYLANTIHHYVTKHPESILAKAVPESYQLEVDYAEFLDDALDDAYELRDEVNQGDKVWILKPSMSDKGQGIRIFRTLDQLQEIFNSFEEESDDEDEDGDAENGEAEDNNGIILSQLRHFIVQEYKSSPLLLPSYDNKKFHLRTYVVCKGDLQVFVYENILTLFADKPFELPEEEEDEISMAGHLTNTCLQGEDPLVVPFWKLDGVSESEKQTIFQQICDIVKELFKAATSVDKMNFQPLANAIEIFGVDFLVNDDHSVSLLEVNSYPDFKQTGDDLKELIYELFDRVITEVVDPMIRGDGNDVTDSTLVEVLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT13</accession>
    <name>G3AT13_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_63089</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tRNA(His) + L-histidine + ATP = L-histidyl-tRNA(His) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="2"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377205.1">
        <property type="nucleotide sequence ID" value="XM_007377143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT13"/>
    <dbReference type="FunCoup" id="G3AT13">
        <property type="interactions" value="885"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AT13"/>
    <dbReference type="GeneID" id="18874589"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63089"/>
    <dbReference type="eggNOG" id="KOG1936">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025113_4_0_1"/>
    <dbReference type="InParanoid" id="G3AT13"/>
    <dbReference type="OMA" id="YQIQKVW"/>
    <dbReference type="OrthoDB" id="1906957at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000021">
        <property type="entry name" value="Probable histidine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476:SF7">
        <property type="entry name" value="HISTIDINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476">
        <property type="entry name" value="HISTIDYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core" evidence="11">
        <location>
            <begin position="59"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="127"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="328"/>
            <end position="329"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001549-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="442" mass="48200" checksum="9A2442C2CF569B2D" modified="2011-11-16" version="1" fragment="single">MSTAPVTSNSKPSTTEAAAVAAAASSAPANSGAPSGKAQSKKSKKSKAQEFLLKTPKGTKDWADKDMVIREAIFGSLSSLFKKHGGVTIDTPVFELREILTGKYGEDSKLIYNLEDQGGELTSLRYDLTVPFARFVACNNISSIKRYHIAKVYRRDQPAMTKGRMREFYQCDFDIAGQYDIMVPDSEILNLLCEGLVGLGIQDFKVKLNHRKILDGIFQACGVKDEDVRKISSAVDKLDKSPWEAVKKEMVVEKGQTEEVADKIGEFVKLNGTIREVVNLLKQSELLAANASAQKGIEEMEILADYVEAFEIDNFLSFDLSLARGLDYYTGLIYEAVTAASAPPANASELKEKATKDKKEDVEDASEYVGVGSIAAGGRYDNLVGMFSNGKSIPCVGISFGVERLFSIIKARAAKELEKISPNHTEVFVMAFGGGEGWNGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT96</accession>
    <name>G3AT96_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Gly-X carboxypeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_141672</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376892.1">
        <property type="nucleotide sequence ID" value="XM_007376830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT96"/>
    <dbReference type="FunCoup" id="G3AT96">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AT96"/>
    <dbReference type="GeneID" id="18870389"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141672"/>
    <dbReference type="eggNOG" id="KOG2275">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_11_0_1"/>
    <dbReference type="InParanoid" id="G3AT96"/>
    <dbReference type="OMA" id="ICPLAPK"/>
    <dbReference type="OrthoDB" id="3064516at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05674">
        <property type="entry name" value="M20_yscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000098">
        <property type="entry name" value="Gly-Xaa carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017141">
        <property type="entry name" value="Pept_M20_carboxypep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047177">
        <property type="entry name" value="Pept_M20A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE/HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962:SF1">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE_HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037217">
        <property type="entry name" value="Carboxypeptidase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 18" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10 16" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="17">
        <location>
            <begin position="246"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="511"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW30859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="543" mass="61458" checksum="4572BAA76F71C094" modified="2011-11-16" version="1">MYVCKTLYHSSIFTRSSLCPLYEPIAPSSFHRDDSKVLSILFDNKYRKQSIERLSGAIQIDTQISDHLPDVPDSPETWEKFKNFHQYLEEKFPLVYENLEVERVNTYGLIFHWKGLSPKLKPVLLMAHQDTVPVQKDTIADWSYPPLSGHYDGQYIYGRGAADCKNVLIAILETLELLISQDYQPRRSIIAAFGFDEESSGYLSAGKIAKRLEKKFGKDSMYAIIDEGNGLTVDSLTNTIVAIPGTGEKGYVDISVELTTPGGHSSIPPDHTSIGIVAELAYLIEKDPYKPVLTNKNPTLGYYQCLASHDSKKKIDFKFKKTILKAGSSRRANNKLIKYITRNKLTRYLIGTSQALDIIRGGEKNNALPEVTKLLVNHRVAIESNIDEIKEHFVKRVIKLAKKHKLSVKAFGKRVYGQSNNEAGEFVITESGNLPPAPPTPLNDKVWAYLAGVTRHVFEDLVFPELGYPIITSPAIMTGNTDTRHYWNLSKNIFRYSPYFVNNFLHDTNAHSVDEKLNFDGHLQLHAFFYEYLQAIDTKEANN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATA4</accession>
    <name>G3ATA4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YCII-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62773</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376900.1">
        <property type="nucleotide sequence ID" value="XM_007376838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATA4"/>
    <dbReference type="GeneID" id="18874458"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62773"/>
    <dbReference type="eggNOG" id="ENOG502S4AZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110355_2_2_1"/>
    <dbReference type="InParanoid" id="G3ATA4"/>
    <dbReference type="OMA" id="FAKEGIW"/>
    <dbReference type="OrthoDB" id="5519740at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1060">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051807">
        <property type="entry name" value="Sec-metab_biosynth-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33606">
        <property type="entry name" value="PROTEIN YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33606:SF3">
        <property type="entry name" value="PROTEIN YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="12440" checksum="B089738AE0D6857B" modified="2011-11-16" version="1">MSIEYNVIIYDNPHADRSKVRAEHVANIPSNVPSPILSAGAIYHDDAKTQFAGSAFHLVANSREEVLEFLKKDVYYREGIWDIDNVIINPIGVAVRLPKKMEGVDASLYKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATL3</accession>
    <name>G3ATL3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 5B</fullName>
            <ecNumber evidence="3">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Translation initiation factor IF-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_62879</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="3"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377009.1">
        <property type="nucleotide sequence ID" value="XM_007376947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATL3"/>
    <dbReference type="FunCoup" id="G3ATL3">
        <property type="interactions" value="492"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATL3"/>
    <dbReference type="GeneID" id="18874506"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62879"/>
    <dbReference type="eggNOG" id="KOG1144">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002656_3_3_1"/>
    <dbReference type="InParanoid" id="G3ATL3"/>
    <dbReference type="OMA" id="FRQSKPA"/>
    <dbReference type="OrthoDB" id="4928at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03703">
        <property type="entry name" value="aeIF5B_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000013">
        <property type="entry name" value="eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000026">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000112">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029459">
        <property type="entry name" value="EFTU-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003078">
        <property type="entry name" value="PRK04004.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14578">
        <property type="entry name" value="GTP_EFTU_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="15">
        <location>
            <begin position="97"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="43"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW30976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="695" mass="77312" checksum="8CCD9B7B9E3CD5C5" modified="2011-11-16" version="1" fragment="single">MALDDDEPLADDWEAALDDEAEAQEEEPEAEEEEEQEDDDEAEIKAKEEEEAAAKAEAERQAKIEAERKAKEEAERKAKEAASKATVKAATPSDKDLRSPICCILGHVDTGKTKLLDKIRQTNVQGGEAGGITQQIGATYFPVEAIKQKTAVMAQYEKQTFDVPGLLIIDTPGHESFTNLRSRGSSLCNIAILVIDIMHGLEQQTLESIRLLRDRKAPFIVALNKIDRLYDWKEIPNNSFRDSFAKQAKSVQAEFHNRYEQIKLALAEQGLNSELYFQNKNMAKYVSIVPTSAVTGEGVPDLLWLLLELTQKRMSKQLMYLSKVEATILEVKVVEGFGYTIDVVLSNGILREGDRVVLCGLNGPIATNIRALLTPPPSRELRIKSEYIHHKEVKAALGVKIAANDLEKAVAGSRLIVVGEDDDEEEIMDEVMDDLTGLLDSVDTSGKGVVVQASTLGSLEALLDFLKDMKIPVMSIGLGPVYKRDVMKATTMLEKAPELAVMLCFDVKIDKEAEQYADEQNIKIFNADIIYHLFDAFTAYQQKLLEARRKEFMEYAVLPCVLKTIQIINKRNPMIIGVDVVEGAVRIGTPICAVRTDPVTKQPNVMVLGKVTSLEVNHKSVDSVKKGQTAAGVAMRLENPSSAQPTWGRHVDETDNLYSLISRRSIDTLKDPAFRDTVSRDDWLLIKKLKPIFDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATV3</accession>
    <name>G3ATV3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_63187</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SUN family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377300.1">
        <property type="nucleotide sequence ID" value="XM_007377238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATV3"/>
    <dbReference type="FunCoup" id="G3ATV3">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATV3"/>
    <dbReference type="GeneID" id="18874632"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63187"/>
    <dbReference type="eggNOG" id="ENOG502QPVV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033459_2_0_1"/>
    <dbReference type="InParanoid" id="G3ATV3"/>
    <dbReference type="OMA" id="CSYACQS"/>
    <dbReference type="OrthoDB" id="5339822at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051526">
        <property type="entry name" value="Beta-Glucosidase_SUN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005556">
        <property type="entry name" value="SUN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31316">
        <property type="entry name" value="BETA-GLUCOSIDASE-LIKE PROTEIN NCA3, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31316:SF0">
        <property type="entry name" value="SECRETED BETA-GLUCOSIDASE SIM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03856">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442615" evidence="12">
        <location>
            <begin position="18"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="79"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="82"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW30329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="395" mass="40786" checksum="8BD2BCC35D8EE9F6" modified="2011-11-16" version="1">MKFSKATVLAFAALALAAPAAKRDDGTDCSSTSFHGHHKHKRAVVYDYAYVTVTVDSNGKPINVPTSTLVASVVPPSADAIQPESSAPAPSSSAVASSSAAPAPESSSAPAPQHSSAPAPVNSGIQGDLSPYQSPSEEFPDGVYSCDTFPAGQGVIALDQLGFGGWSGIYHSDTSTGGSCTEGAYCSYACQSGMSKTQWPDSQPANGVSVGGLLCKGGKLYKSNSRSNYLCEWGVDKASVVSNLDQTVAICRTDYPGTENMVIPTVVGGGQSSPITVVDQSSYYRWQGGLTSAQYYVNNAGVDWQEGCAWGSSGSGLGNWAPLNFGAGYDNGIAYLSLIPNPNNRDSLNFNVKIVAEEGSTVSGQCVYENGQYNGNGADGCTVGVTSGKADFVLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATW6</accession>
    <name>G3ATW6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RNA polymerase II subunit B1 CTD phosphatase RPAP2 homolog</fullName>
            <ecNumber evidence="12">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_63196</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Putative RNA polymerase II subunit B1 C-terminal domain (CTD) phosphatase involved in RNA polymerase II transcription regulation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 12">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 12">Belongs to the RPAP2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="12"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377313.1">
        <property type="nucleotide sequence ID" value="XM_007377251.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATW6"/>
    <dbReference type="FunCoup" id="G3ATW6">
        <property type="interactions" value="336"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATW6"/>
    <dbReference type="GeneID" id="18874637"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63196"/>
    <dbReference type="eggNOG" id="KOG4780">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086709_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATW6"/>
    <dbReference type="OMA" id="CCKEHYQ"/>
    <dbReference type="OrthoDB" id="2590500at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043175">
        <property type="term" value="F:RNA polymerase core enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008420">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039693">
        <property type="entry name" value="Rtr1/RPAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007308">
        <property type="entry name" value="Rtr1/RPAP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038534">
        <property type="entry name" value="Rtr1/RPAP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14732">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT B1 CTD PHOSPHATASE RPAP2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14732:SF0">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT B1 CTD PHOSPHATASE RPAP2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04181">
        <property type="entry name" value="RPAP2_Rtr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51479">
        <property type="entry name" value="ZF_RTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RTR1-type" evidence="13">
        <location>
            <begin position="50"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW30342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="271" mass="31215" checksum="B80465533092AEF3" modified="2011-11-16" version="1">MELLTLDQFIHYLEPYANKETLNPIEASKLQLLITELLVDYYVDFQVLKFISRFLTQQSYDEIIEERNIEHQCGYITCDQSPKQMVRRRSSSSTNGISIVSETDTAYQYQIYNRKPTMILPNTYLSQYCCKEHYQASIFYRNQLSNEAIFARKDIMVVPPFSVTGTWWYENSITCLEEVIAKHKELKQQGKSLSQVIAMMSGLSVDDGNHEADESTNQLVQLIEDFEIVEKESNLVGDVLPEEEEEDVKDGSVNNIEGYVTNSKSFGGYVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATX3</accession>
    <name>G3ATX3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">General transcription and DNA repair factor IIH subunit TFB4</fullName>
            <shortName evidence="14">TFIIH subunit TFB4</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 14">RNA polymerase II transcription factor B subunit 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_63205</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 14">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the 7-subunit TFIIH core complex composed of XPB/SSL2, XPD/RAD3, SSL1, TFB1, TFB2, TFB4 and TFB5, which is active in NER. The core complex associates with the 3-subunit CTD-kinase module TFIIK composed of CCL1, KIN28 and TFB3 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the TFB4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377320.1">
        <property type="nucleotide sequence ID" value="XM_007377258.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATX3"/>
    <dbReference type="FunCoup" id="G3ATX3">
        <property type="interactions" value="1170"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATX3"/>
    <dbReference type="GeneID" id="18874640"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63205"/>
    <dbReference type="eggNOG" id="KOG2487">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040211_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATX3"/>
    <dbReference type="OMA" id="QGCDITS"/>
    <dbReference type="OrthoDB" id="17307at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000112">
        <property type="term" value="C:nucleotide-excision repair factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004600">
        <property type="entry name" value="TFIIH_Tfb4/GTF2H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12831:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12831">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH TFIIH , POLYPEPTIDE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03850">
        <property type="entry name" value="Tfb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU368090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW30349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="367" mass="40085" checksum="ABA990006DCDF35F" modified="2011-11-16" version="1">MEAISERVFTEPTSSSEAISDEPSLLTIILDISPLGWYKLRDLMTLEEMTKNLLVFLNAHLALNNSNQVAFIASSPKGSRFLYPNPAKSYQEVKVSDGATSARDFVNKGMYRQFRIVDKAVLEELNEFLNEINKDQDDTMLADPASKLSGALSMALTYTNRMLTLDQSITTTTASAIASTTSSSASAASSTATGAGGASSGSSSTSMKSRILVVSANDDNDVKYIPIMNTIFAAQKMKLSIDIIKLGDEENSSYLQQPSDATNGIYLHLTDPRGIIQVLSTAYFIEPSLRPFIILPTNSNVNYRASCFITGKAVDVGYVCSVCLCIMSIIPDSKKCPTCNSQFDERIIHQLTKEPEVVKKKRKIDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUJ0</accession>
    <name>G3AUJ0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-conjugating enzyme E2 1</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">E2 ubiquitin-conjugating enzyme 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_63387</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="9">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377517.1">
        <property type="nucleotide sequence ID" value="XM_007377455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUJ0"/>
    <dbReference type="FunCoup" id="G3AUJ0">
        <property type="interactions" value="1190"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUJ0"/>
    <dbReference type="GeneID" id="18874724"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63387"/>
    <dbReference type="eggNOG" id="KOG0418">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_13_1_1"/>
    <dbReference type="InParanoid" id="G3AUJ0"/>
    <dbReference type="OMA" id="HWTFVYA"/>
    <dbReference type="OrthoDB" id="9993688at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016050">
        <property type="term" value="P:vesicle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14311">
        <property type="entry name" value="UBA_II_E2_UBC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23800">
        <property type="entry name" value="UBCc_UBE2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000080">
        <property type="entry name" value="UBC1p Ubiquitin-conjugating enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000037">
        <property type="entry name" value="ubiquitin-conjugating enzyme E2 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015368">
        <property type="entry name" value="UBA_C_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09288">
        <property type="entry name" value="UBA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="10">
        <location>
            <begin position="2"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="8">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="222" mass="24802" checksum="EF76FB660769F4C8" modified="2011-11-16" version="1">MSRVKRIAKELEECRQDSQSGVILKLNNENDLTHLTGYFKGPPGTPYQGGVFQVNIDIPNEYPFKPPQMKFTTKIYHPNISSVTGAICLDILKDAWTPILTLKSSLISLQSLLQSPEPNDPQDAEVAKHYLSNKAGFEETAAYWTKLYASPEDGGEFGSVQVSDSALYGIDDEIVGQFENMGFPRDKTIEVLRRMGVKSFKGVGNKPELENKILEELLKECQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUJ4</accession>
    <name>G3AUJ4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Nucleolar GTP-binding protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_157732</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 8">GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation.</text>
    </comment>
    <comment type="function">
        <text evidence="1">May be involved in the mitochondrial lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 8">Nucleus</location>
            <location evidence="3 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class YlqF/YawG GTPase family. NOG2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377519.1">
        <property type="nucleotide sequence ID" value="XM_007377457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUJ4"/>
    <dbReference type="FunCoup" id="G3AUJ4">
        <property type="interactions" value="897"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUJ4"/>
    <dbReference type="GeneID" id="18871096"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157732"/>
    <dbReference type="eggNOG" id="KOG2423">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011106_4_0_1"/>
    <dbReference type="InParanoid" id="G3AUJ4"/>
    <dbReference type="OMA" id="RTQGFNH"/>
    <dbReference type="OrthoDB" id="444945at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01858">
        <property type="entry name" value="NGP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000559">
        <property type="entry name" value="Nuclear/nucleolar GTPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024929">
        <property type="entry name" value="GNL2_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012971">
        <property type="entry name" value="NOG2_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF9">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08153">
        <property type="entry name" value="NGP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="10">
        <location>
            <begin position="213"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="481"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="501"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="516"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="542" mass="61706" checksum="2302F013FC515BDE" modified="2011-11-16" version="1">MGTQKKEKQRRVRQNDTRDGNLRVKGENFYRDAKKVKHLNMYKQGRAIRNARGDIIKAADLQSTDAPTARVDPNRKWFGNTRVIGQDALTHFREAMGDKKNDSYQVLLKRNKLPMSLLDEKDTSESPAAKIVETESFASTFGPKQQRKKPRVVAASSLEELAEMTESDSVKYQETQELNATLGLMGGSFLDKEDFTQEAKEAIFHKGQSKRIWNELYKVIDSSDVVIHVLDARDPLGTRCESVEKYIRDECPHKHLIYVLNKCDLVPTWVAAAWVKHLSKSYPTLAFHASITNSFGKGSLIQLLRQFSTLHSDRKQISVGFIGYPNTGKSSIINTLRKKKVCTVAPIPGETKVWQYITLMKRIFLIDCPGIVPPSSKDTEADILFRGVVRVEHVSHPEQYIPILLQKCQRKHLERTYEVKGWSQFEDDASLLEQASTEFIELIARKSGRLIKGGEPDESGVSKQILNDFNRGKIPWFVMPPQDEEERTGEDKKAGYKRKRAEVEERNEKKRKLELEAEAEAEAEEYNKEEEEAAAEGSSESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUK1</accession>
    <name>G3AUK1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Acyl CoA ligase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_157748</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377528.1">
        <property type="nucleotide sequence ID" value="XM_007377466.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AUK1">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUK1"/>
    <dbReference type="GeneID" id="18871098"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157748"/>
    <dbReference type="eggNOG" id="KOG3628">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000737_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUK1"/>
    <dbReference type="OMA" id="LVWTYWT"/>
    <dbReference type="OrthoDB" id="69964at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056881">
        <property type="entry name" value="Mug62_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22754:SF32">
        <property type="entry name" value="DISCO-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22754">
        <property type="entry name" value="DISCO-INTERACTING PROTEIN 2 DIP2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23024">
        <property type="entry name" value="AMP-dom_DIP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24919">
        <property type="entry name" value="Mug62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="2">
        <location>
            <begin position="154"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Meiotically up-regulated gene 62 protein-like alpha-beta" evidence="4">
        <location>
            <begin position="664"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="2">
        <location>
            <begin position="901"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="3">
        <location>
            <begin position="1401"/>
            <end position="1507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1364"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1369"/>
            <end position="1382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1591" mass="177554" checksum="A62F24E9090524DC" modified="2011-11-16" version="1">MSHSGSGHSSRQYLGTGQNPATNSSGGVAGGAGNSAPGDSLGINNQPQVLTGASPNIVAGGTTSAVDIPPDMAVLLSKLDQDFMVDKTIDQWTYINRREEIMQSITRLHQRQQQQQSDDWAVLDPDQLETPLNPRRSFDIQQDSPSDLVNILESRARIYKSETAFMVLDIRGKEVSSITWEKLYLKAVKVCYEILHKLSLTNNDTVVLLYKDGEVAEFVVALFGCFMAGVTAIPIHQDISLTEVLEIIKLTSTKLLLYSETVAKELDRLNTQAQPINWPSKLMRWRTTEFGSAKKSELSHWQMKQSQRKRSNSQTQTQNNQNLAYVEFSRSPLGELRGIALSHRTISHQMNCLNVALSSLPNSDNLQRSYTQYQRNCKVVLATLDIRFSIGIIMGILFTVYSGNVHIWAPQRVMEVQGLYANIISKCKASLLLADYIGLKRVTYDYQQSPNATRYFSKTQRVDFSSVKWVLVNALTIDGEFVEILSQRYLRPLGCQRPDNAVIPMLTLSDYGGMVISLRDWIGSQEKLGVPLGDDDDSSDLSSVLIDKEALSRNIVKIVETNPSAFDDISHDLLRVDAFGYPIPDATLAVVNPESSILVSKGELGEIWIDSPCLSGGFYGLRKESKSIFRARCRGAYGYLEMEFLRTGLLGFTYNGKVYVLGLYEDRIRQRVSWIDQKLYKKLNRDLIIGNNGSRYHYSSHLLATLAREVKQVYDCTIFDIFIGNEYLPVAIVEAEVIRKTVEDSNQDSNEAADDYVSSSAVPLNEPVLNAIAQKCFDTLYRRHFLRLYCVLVVDCDTLPKIMRSGGREIANMLCKKRFLEGSLRAEFVKFFVRKSISMIPHGEDIIGGIWSPYVSELRSMALANFPNQYSNVDYRDSSLDDKTGAPLTDFKTIIDILKFRVARSGDSIAFHNLDNGSSSSKSKPLTWKKLEHRAYSVCHYLIEKASIKPGQYVILMYSLSEEFVVAVYACLICGIIPIPMLPFDSNRIGEDFPAFVGVIKDFDVSEILVNDEVEKFLKNGPVADSLKKISHKKVKALRIKNTVKLTKLSNIASLNSKIATYQAAVNFRDSHTTAMVWLNFTSDHYRVGTVLSHSNIIGMCKVFKETCNLNSKTPIVGCVRHASGIGFIQACLLGVFLGTTTYLVSPVSYAENPLQFFLTLARYKVKDAFVTEQMLKYAAIKFSPKGFNLSNLKNMMISTENRRVEIDFLRKISKVFSPTKLSAAAMSTVYNHYFNPMISTRSYMTVAPVDLYLDPLALRQGYVSVVNQADFPNALHIQDSGMVPVCTEIAIVNPETRRVCKEGEFGEIWVKSDANLNSFTNGPKGPVDSLSQAQFNAQIVGGDPQSKYARTGDLGFLHHISITKNDGGENGQEGGEAGGGRRSSNITSFQPLFVLGKIADTFEVMGLHHFPIDIENTIESCHSDIYNNGSCVFKCSDYTIVVCESKRTRYYSSLVPLIVNTVFSKHHIIIDIVAFIKKGEFPISRLGTKQRARIVDAWVQGVIPLSASYGVNYGENSMIKLIKDIDVVAREDPVMGLKNPALSYYDTDMDDDVFADDRQPLTLNQAQPSGNAVFNNSGAFESNSTSEIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUN5</accession>
    <name>G3AUN5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Man1/Src1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_143881</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus inner membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377562.1">
        <property type="nucleotide sequence ID" value="XM_007377500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUN5"/>
    <dbReference type="FunCoup" id="G3AUN5">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUN5"/>
    <dbReference type="GeneID" id="18870580"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143881"/>
    <dbReference type="eggNOG" id="ENOG502QVG5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010838_0_1_1"/>
    <dbReference type="InParanoid" id="G3AUN5"/>
    <dbReference type="OMA" id="KWECGEL"/>
    <dbReference type="OrthoDB" id="2503928at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071763">
        <property type="term" value="P:nuclear membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12935">
        <property type="entry name" value="LEM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1180">
        <property type="entry name" value="MAN1, winged-helix domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025856">
        <property type="entry name" value="HeH/LEM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044780">
        <property type="entry name" value="Heh2/Src1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018996">
        <property type="entry name" value="Man1/Src1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041885">
        <property type="entry name" value="MAN1_winged_helix_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47808">
        <property type="entry name" value="INNER NUCLEAR MEMBRANE PROTEIN HEH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47808:SF2">
        <property type="entry name" value="LEM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12949">
        <property type="entry name" value="HeH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09402">
        <property type="entry name" value="MSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="387"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="HeH/LEM" evidence="10">
        <location>
            <begin position="4"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="Man1/Src1-like C-terminal" evidence="9">
        <location>
            <begin position="396"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="97"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="130"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="193"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="210"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="237"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="250"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="330"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="346"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="736" mass="83237" checksum="120811336F9A07DB" modified="2011-11-16" version="1" fragment="single">GFDPKKLKVAELRNILNRHGVEYASNARKTQLVELFQDNITTKRDELIKELNVKGSDEGIVSVDLDSSTTAEENSGGNMDEAGGAVVLEKIEDSRQEVFSPNSVKIPNFSPTTKTSPKVSSETKKRKLEEDEDKPDASVEEETPSKKIPKRLKPIKHEIPKRTIFDDTESDIDMEVFPKVSPATTKHEKRTPKSSSKATPNSSKGSHNISPKTTPSKVSKPSSKKTTPHKASSSKSANTSVNESFQSIAEEEHHFDQELSKIKEKAASPSADEVEFAKSLGITIEGFQPPQPPFAEPASHLETPKKSPVFDRSFLTPKPRLIPISTKDTDESDDDDDDDDEEEEKTEVQKVVVEEKQGKVQTTTTSELPELSQEHSQSLSRRSLTQALMTFFIWAIIVASGLFFYWYIEQSYMVGYCGQEIHNETFGNIENPILRQVGKFLDDNAKPSCVPCPPHGRCFPNLELACYEDFIEYRPWNNFIKPGNKKCIPDTKKAEKLEIMMEVALDLIRSKNAQVQCGRGVDDFESGISVQDLHDLLLEMKAPYITLEEFEELWERSVVELEKEPEIIVRQKSPNFPKEFSNINDSNSHEETDKVLRSTSLSNISLNCQISNSLMGGLAQYKPHLFICTALFILFKYVQYQYNQYRLQLLKVDILHREVVSKLVNQMKQFQSGEAGFGYIGANQLRDLILASEDNLKSRIKLWGLVSEKVEKNTNVKVEVIEHHGEMMKIWKWIGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV07</accession>
    <name>G3AV07_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Efficient mitochondria targeting-associated protein 19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_57543</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM97/sigma-2 receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377847.1">
        <property type="nucleotide sequence ID" value="XM_007377785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV07"/>
    <dbReference type="FunCoup" id="G3AV07">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV07"/>
    <dbReference type="GeneID" id="18872126"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57543"/>
    <dbReference type="eggNOG" id="ENOG502S75H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086812_4_0_1"/>
    <dbReference type="InParanoid" id="G3AV07"/>
    <dbReference type="OMA" id="FIWIEIL"/>
    <dbReference type="OrthoDB" id="433124at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033118">
        <property type="entry name" value="EXPERA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051987">
        <property type="entry name" value="Sigma-2_receptor-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016964">
        <property type="entry name" value="Sigma2_recept"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31204">
        <property type="entry name" value="SIGMA INTRACELLULAR RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31204:SF1">
        <property type="entry name" value="SIGMA INTRACELLULAR RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05241">
        <property type="entry name" value="EBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031032">
        <property type="entry name" value="TMP_97_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51751">
        <property type="entry name" value="EXPERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="EXPERA" evidence="9">
        <location>
            <begin position="4"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR031032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="159" mass="18525" checksum="1DC464F2401F6527" modified="2011-11-16" version="1">MKTVDKIFLIYFISHIPITIFMDSTIIVPKEYQLELSKTLLDFHISANKDILLIHSPTWFKLFGVVELVFQLPLFFYFIYKLLVAPVLDSDYFIWTIVYGFNASFTTFVCMGWIIIEGQDLGLNLQEIANLCAIYSPYLIIPAWLLLNSISRLRKIKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVK1</accession>
    <name>G3AVK1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Translation initiation factor IF-2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_57446</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377716.1">
        <property type="nucleotide sequence ID" value="XM_007377654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVK1"/>
    <dbReference type="FunCoup" id="G3AVK1">
        <property type="interactions" value="500"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVK1"/>
    <dbReference type="GeneID" id="18872120"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57446"/>
    <dbReference type="eggNOG" id="KOG1145">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006301_5_2_1"/>
    <dbReference type="InParanoid" id="G3AVK1"/>
    <dbReference type="OMA" id="RKNPWMN"/>
    <dbReference type="OrthoDB" id="361630at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03702">
        <property type="entry name" value="IF2_mtIF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03692">
        <property type="entry name" value="mtIF2_IVc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000126">
        <property type="entry name" value="Mitochondrial translation initiation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000008">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000001">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000019">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_B">
        <property type="entry name" value="IF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053905">
        <property type="entry name" value="EF-G-like_DII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044145">
        <property type="entry name" value="IF2_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006847">
        <property type="entry name" value="IF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000178">
        <property type="entry name" value="TF_IF2_bacterial-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF20">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22042">
        <property type="entry name" value="EF-G_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04760">
        <property type="entry name" value="IF2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01176">
        <property type="entry name" value="IF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tr-type G" evidence="13">
        <location>
            <begin position="220"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="34"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="490"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="48"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="76"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW29950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="752" mass="83296" checksum="F063FA453EFFB53B" modified="2011-11-16" version="1">MMLQRCCNRSIGQVPNLLRALALAPVQQHRCYADGPGKPKVNRFAAKYSNSPPAGRSNGQNNQDRPRYNPPAQSRPPRNNQSNGPSRSQSNTQSRPQSNTQSNTQSRPQSNPQSNRHDHTQTQPARHSQPQKPVKKKIVKIRIPPYITIANLASVMNVRLNDVFKKLTLLGYEDVRHDYILDKENAAMIADEYGIEVIASEGSDSDDLFSAPIKEEFLKERPPVVTIMGHVDHGKTTILDYLRKSSIVDTEFGGITQRIGAFSVTTPVSNKRITFLDTPGHAAFLKMRERGAIVTDIVILVVAADDSVMPQTIEAIKHAKKSGVPIIVAINKCDKRDVNVDRVLADVSAQGVDIEDYGGDTQTVKVSGKTGLNMDKLEEAVITLSELSEFKSEEKGVPAEGWVIESEVAKGLGNVATVLVRRGTLKTGDIIVAGNTYCKIRGMKNEYGKPVKTAGPSTPVQIFGWKELPDSGEQVLQAKSESIAKKVIDYRESRAKEIQAEREVEEINIKRQEAIKESARLEQIEKLKLAGLDYSELENEGGESKTKICNYIVKSDFFGSAEAITESLDGLGNDEVQAKVIFHEAGAPTDSDVELAKTADATIISFNVKVPKQVSTKADAAKVAIKEHNIIYRLIEEVTEELCSHLAPRIEYKYHAEAKVLKVFKFSGKAKKLVTAGCRVTSGEFKRNCSVKVFRGKEEVYDGQLSSLKHEKDDVASVKNGSECGLTFEDWNDFKEGDVVKYYERIEHKRYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVN2</accession>
    <name>G3AVN2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_63810</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377963.1">
        <property type="nucleotide sequence ID" value="XM_007377901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVN2"/>
    <dbReference type="FunCoup" id="G3AVN2">
        <property type="interactions" value="710"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVN2"/>
    <dbReference type="GeneID" id="18874926"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63810"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026447_2_0_1"/>
    <dbReference type="InParanoid" id="G3AVN2"/>
    <dbReference type="OMA" id="TNAADAW"/>
    <dbReference type="OrthoDB" id="1049195at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000362">
        <property type="entry name" value="GBP2p Poly(A+) RNA-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="111"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="229"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="351"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="186"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="296"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="8"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="39"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="186"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="304"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="433" mass="49671" checksum="E1BEAD2FBD1DA160" modified="2011-11-16" version="1">MEGMDIDDYSRDRSRSPVRERSDNNDVESSAASTTSSYSRRDYSYRSDRSGYGNERRGGGRRDHGSYDRHDRGRRDYGRGRSRDYSSRAEYSSGGTSDESYRAKTERNYDNSIFIGNIPFDCSDKDVADIFSKDFTIVRSDIVTNRGRSRGMATIEFNNKEDVRSAIEKFDRSEYRGREIFVRQDYPPPEKKEEFRERRETYDNRRDKSYESRRRDQHQQHQQSSKPGCEIFVGNLPFSINWQALKDLMRKAGDVIRADVRMDNWGKSRGFGTVVFGTEEEANKAVEMFQGYEIEGRKLDTRPGRNQSSASNGSNTSDRSSYRERERASSQPVVKPSEFTDGVTAGGERSDTIYVSNLPFATQNDDLFELFETVGRTTKAEIILDPITGRPSGNAVVQFELAELSDNAIENLNNYLYGGRNIQISYAKRPEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVU4</accession>
    <name>G3AVU4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cell differentiation protein rcd1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_63613</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT9 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377755.1">
        <property type="nucleotide sequence ID" value="XM_007377693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVU4"/>
    <dbReference type="FunCoup" id="G3AVU4">
        <property type="interactions" value="880"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVU4"/>
    <dbReference type="GeneID" id="18874833"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63613"/>
    <dbReference type="eggNOG" id="KOG3036">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039962_2_0_1"/>
    <dbReference type="InParanoid" id="G3AVU4"/>
    <dbReference type="OMA" id="EKVYTWI"/>
    <dbReference type="OrthoDB" id="1183224at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000160">
        <property type="entry name" value="Cell differentiation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007216">
        <property type="entry name" value="CNOT9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12262">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04078">
        <property type="entry name" value="Rcd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW29989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="34916" checksum="513281D617E2B457" modified="2011-11-16" version="1">MYNTAQAKGPIQTMGQMGTPSREESGLATNKALNDEQIYQWISELVTGTNRERALLELGKKREQYDDLALVLWNSFGVMTVLLEEIISVYPYLNPPNLSASVSNRVCNALALLQCVASNVQTRGLFLSANLPLYLYPFLSTNARQRSFEYLRLTSLGVIGALVKNDTPEVINFLLTTEIVPLCLNIMEISSELSKTVAIFILQKILLDDQGLSYVCTTFERFHTVASVLSKMIDQLSISASSQNPQQPSNSSGRLLKHVVRCYMRLSDNLEARKALSSILPEPLRDGTFSLILQDDVATKRCLAQLLSNISESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVX4</accession>
    <name>G3AVX4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GLC7-interacting protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_144212</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377785.1">
        <property type="nucleotide sequence ID" value="XM_007377723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVX4"/>
    <dbReference type="FunCoup" id="G3AVX4">
        <property type="interactions" value="293"/>
    </dbReference>
    <dbReference type="GeneID" id="18870607"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144212"/>
    <dbReference type="eggNOG" id="ENOG502QYHN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007231_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVX4"/>
    <dbReference type="OMA" id="KWGFFVQ"/>
    <dbReference type="OrthoDB" id="8436363at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113:SF12">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="64"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="205"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1098"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1169"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="99"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="156"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="176"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1108"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1205"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1225"/>
            <end position="1237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1237" mass="136939" checksum="CE5DA58F6E79D874" modified="2011-11-16" version="1">MSDPQLSIDGTLNGKTANLLPSTLRHSHSEEIDNGVSNIDVDWLFRSRSQKLSKKVNKLNNSKLALDTDVAKQDKKQTPPNTPKSEPKSTEDSKIVTPIDQTKNTPVNGTAKSASTGGRSRSVSASIVETKSAYLNSDPQPRRGSLNLPLPAQNNASTPSASTSTSTTRRLFGSISSKFKSGSPQSSPTSSPKVAVAKLASASPLKTVPNDLPSNINKPPAGSGIPGAAAQTKRRSSLSLKANSGLFKDSFKDDNAPAPPKASSEGLKFFRRRSSVTPATVPQPSPAAPVIPKRQILNKNANKKTIPIKELQDVHLRRVTFAIDKLEYDPQQQIPSRRPKRGNVIIPQEIAAPPPRLCQGIAMNDASSKDSAQQKQAYTEREIAMALEAQKRALQMAEKHAHDAYMQAKKVAQEVASFKPSKLSKEKKQEQELEEEQELKLLLNKAHAHVDIDKPLHVHEQHFEKEVHDKQGVTLEMIYTRCCHLREILPIPATLKQLTNKTAPLQVLKFLNPKPTLIDVLSFSDFIAITPIYTVIFDNVTMTTEMLKHFLGSLAYNKSLEKLSLRNVAIDDQGWKFLCKFLSRNTTVKKLDISQQKYKPDTNPGLIRANMNWDLFIKAIILRGGIEELVINGCKLTDETFTKLINEAVKKSTRRFGIAGIDLNLTKAEVVAGWLTDPESKCVGVDIASNDLGQGQLIPFINAFNKGTVENLVFFSLNSTQLTDVEQASDLIKALANVKTLRFLDLSSIPHVFPGLIATLAKYLPRYYNLRRIHFDFNELTSKAIGSIAAFLPKVPRLFHVSLLGNRDLSPAAVFTLYTAVKSSNTLHALDLDYDLVSEDLASRIAFYLMRNMDTSLKPEYFEGTAEIDQEDLMFDGSLLMETAEKLLMEVDKGKQEEDAKIQQIITETIMERTRAIRKDIHATIDKLFELRKAGTLSFEGKETLVRFCLLDASLEKLVHMFEEHGTVSMAVTPTSEQPPRVETPPLSRVGSGWRLATLTGLHLHESSSELLTSGPILSAHNINSQQQDYFSSALMSNDQNLHPHQVVVESDGGKDVAVDKTTGRPVLLRSLSQTSLYAKEQELEEGDFLRLGAFLQSRENEEPSSPKSDKLNDKKSEFPLLSGLPSGSELRQAIMGAKGIRNVTELIDKIQTNRITLEKLIPEEGAVMDSEDSKLTTVQSHDDDKDTINTSGSSSKGTDEDTAVDPKVDEVYDKLLNDAEKRVRDKKHHHSHHHHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADV7</accession>
    <name>G3ADV7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">SUMO ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_47769</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIAS family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007372031.1"/>
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    <dbReference type="eggNOG" id="KOG2169">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_255200_0_0_1"/>
    <dbReference type="InParanoid" id="G3ADV7"/>
    <dbReference type="OMA" id="ILKLQMG"/>
    <dbReference type="OrthoDB" id="28127at2759"/>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="entry name" value="PINIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PINIT"/>
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        <property type="entry name" value="PINIT_sf"/>
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        <property type="entry name" value="SAP_dom"/>
    </dbReference>
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        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Znf_MIZ"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF4">
        <property type="entry name" value="TONALLI, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14324">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SAP" evidence="12">
        <location>
            <begin position="26"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="PINIT" evidence="14">
        <location>
            <begin position="127"/>
            <end position="279"/>
        </location>
    </feature>
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        <location>
            <begin position="309"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
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            <end position="458"/>
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    </feature>
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            <end position="519"/>
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            <end position="579"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="11">
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            <begin position="593"/>
            <end position="612"/>
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    </feature>
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        <location>
            <begin position="638"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="680"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
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            <begin position="972"/>
            <end position="1064"/>
        </location>
    </feature>
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            <begin position="1077"/>
            <end position="1184"/>
        </location>
    </feature>
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        <location>
            <begin position="1190"/>
            <end position="1209"/>
        </location>
    </feature>
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        <location>
            <begin position="1272"/>
            <end position="1300"/>
        </location>
    </feature>
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        <location>
            <begin position="1308"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
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        <location>
            <begin position="491"/>
            <end position="519"/>
        </location>
    </feature>
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        <location>
            <begin position="563"/>
            <end position="573"/>
        </location>
    </feature>
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            <end position="650"/>
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    </feature>
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            <begin position="1272"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        </source>
    </evidence>
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        </source>
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    <sequence length="1387" mass="151714" checksum="D0358F66F1EA04BB" modified="2011-11-16" version="1">MTDTTENETEKVQLSTEEIADTLVRLHQMKVAQLKDICRSMELRLSGRKADLIDSIESYFKHGQEFGDNIRLLTIRTLVLRRVNGTNLPTYRELHSAISNGSYSLAGIVGDTNPTAAPSLPQQQATLHSFDSIPHKGHSLYFLESPFYKLRRSIHSSPQLCAPAKGRGECHFQFIFAADEYKLLKDNPGTKVYLLCGKQGTSGPSSTDVPIEYPSPSEIHVNGKQLLTQYKGIKGNIGTAKPADLTDLVKPPPNTNKVTLVYQQTQEAYLAYLYLVQAISPETVLERVKQRPKIHKIATIARIKAENSEDEDIMLESSTVPLTDPVSRTKIKYPIQSIYCNHTQCFDGMSFLQTQVQLPTWSCPVCSKRVKVEDLAISEYFEEILATVEEDVDSVIINADGTWEVEVQPKNEDSDAVGKPGRSASAQVETVEIISLSSDEDEDEQNDEQNEEPEDDSGIIQETAETSIEEPINVLTVKTASMEVAGPTNDDYPTTSTNELTTSVNSGSSADTSGESGLTNIKSSAALDNAIAQMEDTIEEELNDESPLANLRDARDRIRRNHSNSSIENNSENVLPELGNRSPAKTLVTHSLVSPAGSNSVSPTRTSDGPVQLQTQTSAVSANQGIGLSNIALTASINQPPNASQNQPRVANSPPPNVSTHPRSPRYSSIFINPRANQRHLHQMRQATSPRSTSPPLRPSGSSENSPVLDHARSISSKSPDTNISAPQTNSNGVHTIGTNVATSSSPLESTHNSSSPLMANVNLQIPQQTQSSPTSSSPVNSQGPIYPRVSESLASQTYQKKPNGVTSPVQSNGQHPIQPNMSHNSHQQRPHKNAAYYRQLIIALNQRRKANYLRFQQELEAMKKRHEEDIRRFKHETKSKSFEKNEQANRFRKEASMATSREMSNELMAKSDRIRDRVLSYAQERAREIVTMEKEQQSQIQNLNNSFVSDMKHLAQEIEKATRLVMELTRSSGPVGSNLSTNGVGAASSINPSNTTTSGVAASAPTAQSSHHHHHSHSHISVPTQNSQIVRPQMNQQSRSFNPGWSAQSQNLRPEQVRSASQQTFNSNMEQIGWARSRSGPSSVYKPAQPTITTQPVVQPVQPTQLTQPTRPIQSVRPQVTQVPKVHPTQHAPSSSPNLATQQIQSTSALSAQVQPNQTSSVSLQTTVPSKDSSSQESQDAFSHLSSLFEQDNGVKNPAESGQTRPMVENLTTESVQPTAAIHVSQPTNSAQSLNNSPMQNAVDIAQGSSSVPKQVSQQNRQFPSPFFVDTQESNQQKETVSTQNAQSPTISSSVATNVEKRPLPQEPLSSISAFDFPVNPSPPEKRQKITGMLGIELSKDVIGLEGSPQPESFSFGNGKETAAAKNTFALFPGHEVIDLTGDDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADX0</accession>
    <name>G3ADX0_SPAPN</name>
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        <recommendedName>
            <fullName evidence="10">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_130963</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the type 2 lipid phosphate phosphatase family.</text>
    </comment>
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        <property type="protein sequence ID" value="EGW34694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_007372044.1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03388">
        <property type="entry name" value="PAP2_SPPase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF28">
        <property type="entry name" value="DIHYDROSPHINGOSINE 1-PHOSPHATE PHOSPHATASE LCB3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="285"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="460"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="10">
        <location>
            <begin position="118"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="483" mass="54520" checksum="2AD2CDA002A046B4" modified="2011-11-16" version="1">MTSSVREQTVNKRRGDKQSEGKSKSKSHGLADEADHTDDAGNQSNDHYKARLSPFRYSLRSKLLPIIRAESSYLYSLQTKLRHPILDFYFAWTANLASHTFYVLMLPPPIWFGASDLSRDLVYVLGLGIYFTGFLKDFFCLPRPRSPPLRRITMSSYTTQEYGFPSSHSANATAVTLVLISRLVNLDISRGEYWALMSGLILYYFSLIFGRLYCGMHGFLDIFIGSSVGASLFAFRHYWGKQWDEFLFNNALGAIPSAIIIIALFMSFIHVHSEPVDNCPCFDDSVAFIGVLIGLDLSHLVAFKTQYLANLNLHKDPFLLPFDSSQSPWKTVVRFILGVTLVVIWKTISKPVIFTILPPIYKFIGVNLPRKNFIATAHTKAPTRAIRSASISNETGEIGDINTFIKGITAHNQRDEVGPSTEIDYFEMLDYNKEHHLQEKVEEVAGVFKPRYDVEIIGRVIVYAGVSTTAVWGLFMATQYVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADX5</accession>
    <name>G3ADX5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase eIF4A</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Eukaryotic initiation factor 4A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_57763</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. eIF4A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372111.1">
        <property type="nucleotide sequence ID" value="XM_007372049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ADX5"/>
    <dbReference type="FunCoup" id="G3ADX5">
        <property type="interactions" value="1314"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ADX5"/>
    <dbReference type="GeneID" id="18872184"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57763"/>
    <dbReference type="eggNOG" id="KOG0327">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_0_1"/>
    <dbReference type="InParanoid" id="G3ADX5"/>
    <dbReference type="OMA" id="FGCQALV"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000089">
        <property type="entry name" value="Eukaryotic initiation factor 4A-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000031">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15 19" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="18">
        <location>
            <begin position="23"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="54"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="235"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="14">
        <location>
            <begin position="23"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW34699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="400" mass="44935" checksum="5FD096EB465A8761" modified="2011-11-16" version="1">MASEGITEIDAGLIETNYDNVVYKFDDLNLKPNIVRGIFGYGYESPSSIQQRAILPITEGRDVLAQAQSGTGKTATFTIAALQRIDENEKATQALILAPTRELALQIKNVITSIGLYLKVTVHASIGGTSMSDDIEAFKSGVQIVVGTPGRVYDMIERRYFRTDKVKMFILDEADEMLSSGFKEQIYNIFRLLPETTQVVLLSATMPQDVLEVTTKFMNNPVRILVKKDELTLEGIKQFYVNVEQEEYKFDCLVDLYDSISVTQAVIFCNTRSKVEFLTNKLKENKFTVSAIHADLPQADRDTIMKEFRSGSSRILISTDLLARGIDVQQVSLVINYDLPANKENYIHRIGRGGRFGRKGIAINFVTDRDVGMMRDIEKFYSTQIEEMPADVGSLFGYSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEB3</accession>
    <name>G3AEB3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_58854</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373059.1">
        <property type="nucleotide sequence ID" value="XM_007372997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEB3"/>
    <dbReference type="STRING" id="619300.G3AEB3"/>
    <dbReference type="GeneID" id="18872647"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58854"/>
    <dbReference type="eggNOG" id="ENOG502QSS6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030508_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEB3"/>
    <dbReference type="OMA" id="TCCNRIC"/>
    <dbReference type="OrthoDB" id="276515at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09083">
        <property type="entry name" value="EEP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF36">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="92"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="346" mass="41284" checksum="329973A0A0D006B8" modified="2011-11-16" version="1">MTDERIPLLVQVAPRTRKPLRMMCLFILPCILLLFALCSWYKVTHHLGPEVTVRIYTNNIRYDNRNHPDEFEQPWKKRRVQSINSMDFHSSAQANVVCLQEVLHHQLNDILDKLNKHDEWTYYGVGRSDGITRGEYAPILYKKSEWRVVQNTTYWLSETPWKPSRGWDAALERIVTMITLQSYHNPLVKINVFNTHYDHRGKVARRESSKLIVNKMQNFNTYPSFLCGDFNTEPKDEPYHILTKAGFKDARKLVNWDDEYGHSTTFTGFNKEHEADSIIDYIWSPYFTRSDEVETESINYFKLNHHRYYDIVLRQFGILHNYFKGFYFSDHRPVVATYIIRRIRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEH3</accession>
    <name>G3AEH3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcription regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_145138</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372147.1">
        <property type="nucleotide sequence ID" value="XM_007372085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEH3"/>
    <dbReference type="FunCoup" id="G3AEH3">
        <property type="interactions" value="1131"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEH3"/>
    <dbReference type="GeneID" id="18870672"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_145138"/>
    <dbReference type="eggNOG" id="KOG4204">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001360_2_1_1"/>
    <dbReference type="InParanoid" id="G3AEH3"/>
    <dbReference type="OMA" id="MCEEVIK"/>
    <dbReference type="OrthoDB" id="10265969at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032221">
        <property type="term" value="C:Rpd3S complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061188">
        <property type="term" value="P:negative regulation of rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061186">
        <property type="term" value="P:negative regulation of silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016479">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044804">
        <property type="term" value="P:nucleophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070550">
        <property type="term" value="P:rDNA chromatin condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030174">
        <property type="term" value="P:regulation of DNA-templated DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000002">
        <property type="entry name" value="Paired amphipathic helix protein SIN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000003">
        <property type="entry name" value="Paired amphipathic helix SIN3-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.11">
        <property type="entry name" value="Paired amphipathic helix"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013194">
        <property type="entry name" value="HDAC_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003822">
        <property type="entry name" value="PAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036600">
        <property type="entry name" value="PAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039774">
        <property type="entry name" value="Sin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031693">
        <property type="entry name" value="Sin3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346:SF0">
        <property type="entry name" value="SIN3A, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346">
        <property type="entry name" value="SIN3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02671">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08295">
        <property type="entry name" value="Sin3_corepress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16879">
        <property type="entry name" value="Sin3a_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00761">
        <property type="entry name" value="HDAC_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47762">
        <property type="entry name" value="PAH2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51477">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <feature type="domain" description="Histone deacetylase interacting" evidence="7">
        <location>
            <begin position="432"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="46"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="217"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="729"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1166"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="85"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="232"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="753"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="777"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1166"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1188"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1226"/>
            <end position="1260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1260" mass="143792" checksum="059FE4F3E5F293CF" modified="2011-11-16" version="1">MKDFKSQSIDTPGVIDRVSTLFQGHPNLIQGFNTFLPPGYKIECSLDPSDPNPIRVTTPTGTTTRPNLSGNTYSQRWGTEEGASQVQQQQQQQQQQQQQQQQPQQPAPQQVAAQMQYQQEQQVQPGGSNVEFNHAISYVNKIKTRFANQPDTYKQFLEILQTYQREQKPISEVYEQVTVLFANSPDLLDDFKQFLPDTSNQGFQQQQQPVHMQEGSYYVNNGSQLPPVGNFQPPTGTTGLSPSATQQHYGQQISYPSTGSPEPQYGMDQKATKKKKSISEPYYDDNYNQEARLSSIRSGVVATKTGKPPGMVKVVNTSVAAAVNPTLVPGIPEPVPPYASVKTSSLSEEISFFDKVKKAIGNKQSYSDFLKILNLFTQDIIDKDTLVERVQGFIGETHPDLFNWFKMFVGYQDVPQNIENITFKKHQLELSLCKAYGPSYRQLPKAETYMPCSGRDEMCWEVLNDEWVGHPTWASEDSGFIAHRKNQYEEILFKIEEERLEFDYYMESNLRTIQILETIANRIANMTPEQKVAFKLPPGLGHNSQTIYKKVIRKIYGKDRGFEVIDALHENPAIAVPIVLKRLKQKDEEWKRAQREWNKVWRDMEQKVFYKSLDHLGLTFKQADKKLLTAKQLVSEISTVKVEQQNKRLHPLTPKPQEQLNYTFEDTEILFDIVKLADVFINRSSNYSAGDGEKLSQFLQFFVSLFFGIPSETIQNALAARGKAEKNEFVHEEAENSDSSTYSSDAGAKKRARESDLLRDVLKKQSKSKKDDRGDSPTPQSADATITQEVAEELEKSTELWIKSANTKFSLKDDESENKREKYNLFCNTTIYVYFRHLTTLYERLHEIKAMNETVSKEIKSRKHPQFAKDLNLLSHQLEDVGVEINGSKDCYVQVLELSEKLIEGEIEHQWFEESLRQAYRNKAYKVYTVDKVVQALVKHMHSMITDAKTSEMMVLFENDRLKPVSSAKEQILYRIQVRALMSPDENMFKISFNETQNSVNIQFVSLDDLTINDHENSQEQYNYYVTSYVLSHPTEGVPASKLNMPFSRSFIESLDEDSVDGRATTGLKVSVCENSYRLFFEENSHDEFIANSVYKERAEESGKPEEKIEKLKKLLNGESGWKSVCADEDASKLEEKIKILYEKGAAEFNEHERAKDVEIKEAAAEAQKQLEEKEASAQQEESIDATSESKPETTDEQQVKTEEEHVQEEGAENLDETQMVDADTTVAQPNDTTIQADANDTTTDETINPEETTIVGQDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEN7</accession>
    <name>G3AEN7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PQ-loop-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_58870</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373075.1">
        <property type="nucleotide sequence ID" value="XM_007373013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEN7"/>
    <dbReference type="GeneID" id="18872656"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58870"/>
    <dbReference type="eggNOG" id="KOG2913">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_585253_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEN7"/>
    <dbReference type="OMA" id="YWYYTRV"/>
    <dbReference type="OrthoDB" id="8048523at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF44">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="419" mass="47363" checksum="81E520C93B77DA01" modified="2011-11-16" version="1">MFDPPYPIIFTAYKATAFVSLTFSLIAFIPQIIQVHRNKSFHHLSVSFIGILLIQDVFNLLQSSLDYQILLYLYYCILDCLLLLQYYYYHHITYSSPLFKAIIVPSLITTVHARPVEIPEQINVNGNSLANALLLVSSRIPQVVKNFNHKSAKGISKNFLVLNILSNLFYVVSLSLSLYLLAYTNNIQDTDEPFGVVLQRQLPLIIASGITILLDLTILYQTHIYQPQIRHPRRLSASTRQPSWYTRNQPIYDSQEFFHPTEDTTMDHDHFLAPILSQQSRGRKLTQSPNSETTSLIMKSSYPSYVSPPPAHYISSSLNSPSKEQFTQTMFSRLAKSIKRYPNSIDTNIGSTNAAMSPTSFIPSIVGHASSVGKKLNDGSKVPFSPSDFLHDDYYWKNSAGSDSMLGRSTDGDRIPILH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEU9</accession>
    <name>G3AEU9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sec1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58985</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373191.1">
        <property type="nucleotide sequence ID" value="XM_007373129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEU9"/>
    <dbReference type="FunCoup" id="G3AEU9">
        <property type="interactions" value="1233"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEU9"/>
    <dbReference type="GeneID" id="18872708"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58985"/>
    <dbReference type="eggNOG" id="KOG1301">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016216_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEU9"/>
    <dbReference type="OMA" id="DAHMSIC"/>
    <dbReference type="OrthoDB" id="10251230at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035543">
        <property type="term" value="P:positive regulation of SNARE complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046578">
        <property type="term" value="P:regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="409" mass="46482" checksum="9209324ECD60E420" modified="2011-11-16" version="1" fragment="single">MSLVETSSLRNRQISILERMLHLNKEGSADLTLASKSEEIIWKVLVLDTKSRAVLSSVLRVNDLLRCGITVHSLINSNRSALPDVPVIYFVEPTIENILLIIDDLVHDKYESFYINFTSSINRELLEEFAKKVSTSGKSTKIKQVYDQYLDFIVTEPNLFSLNLPKIFTTFNTVHTQEETIHQLVDTIADGLLSTIITMDIVPVIRAQQNGPAEFVATSLDLKLRDYLSNSRNAGATQPIQQRPVMILLDRNFDLASMFSHSWIYQCMVSDVFSLQRNTISIHKYSKESTQPTIKKYDVDPRDFFWNKYSQLPFPDVVESADAELNAYKNDAKEVTAKTGITSLQDIDHNTSETANIQQAVEKLPELTARKTTLDMHMDILAALIDELQAKNLDKFFEIEQNASDPKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF80</accession>
    <name>G3AF80_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Protein transport protein BOS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_57961</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">SNARE required for protein transport between the ER and the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 12">Belongs to the BOS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372281.1">
        <property type="nucleotide sequence ID" value="XM_007372219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF80"/>
    <dbReference type="FunCoup" id="G3AF80">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF80"/>
    <dbReference type="GeneID" id="18872270"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57961"/>
    <dbReference type="eggNOG" id="KOG3251">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078260_1_0_1"/>
    <dbReference type="InParanoid" id="G3AF80"/>
    <dbReference type="OMA" id="FCWLVIH"/>
    <dbReference type="OrthoDB" id="158360at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027027">
        <property type="entry name" value="GOSR2/Membrin/Bos1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230:SF1">
        <property type="entry name" value="GOLGI SNAP RECEPTOR COMPLEX MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230">
        <property type="entry name" value="VESICLE TRANSPORT V-SNARE PROTEIN VTI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028865">
        <property type="entry name" value="Membrin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="99"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR028865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW34869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="227" mass="26734" checksum="A1415D77E536B271" modified="2011-11-16" version="1">MNSIYNHGVKQTQTITKDLSEFEQNLSTSPLSLQGAITTSITVFKKTIKEYHDLLEKSKNDPSYPKHESRFQKFSHDVHEFQTKFESLKLQRDTIVQESNKQELFGRRHTHNSTTSSDNPYDTTNQQQQSYMSYQEGLYKEKDALSRGTEQLDHILEMGQQAFEDIVDQNEVLRRLQTKFEESLVVLGVSRGTIRSIERRAKQDKWLFWFCVVVMLVSFYYIVKIFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFF6</accession>
    <name>G3AFF6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">3-hydroxyisobutyryl-CoA hydrolase</fullName>
            <ecNumber evidence="2">3.1.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_58073</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-hydroxy-2-methylpropanoyl-CoA + H2O = 3-hydroxy-2-methylpropanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20888"/>
            <dbReference type="ChEBI" id="CHEBI:11805"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57340"/>
            <dbReference type="EC" id="3.1.2.4"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.2.4" evidence="2"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372357.1">
        <property type="nucleotide sequence ID" value="XM_007372295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFF6"/>
    <dbReference type="FunCoup" id="G3AFF6">
        <property type="interactions" value="719"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFF6"/>
    <dbReference type="GeneID" id="18872308"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58073"/>
    <dbReference type="eggNOG" id="KOG1684">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_22_0_1"/>
    <dbReference type="InParanoid" id="G3AFF6"/>
    <dbReference type="OMA" id="EVFTMEY"/>
    <dbReference type="OrthoDB" id="1737613at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003860">
        <property type="term" value="F:3-hydroxyisobutyryl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045004">
        <property type="entry name" value="ECH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032259">
        <property type="entry name" value="HIBYL-CoA-H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176:SF3">
        <property type="entry name" value="3-HYDROXYISOBUTYRYL-COA HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176">
        <property type="entry name" value="3-HYDROXYISOBUTYRYL-COA HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16113">
        <property type="entry name" value="ECH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl-CoA hydratase/isomerase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="423" mass="47214" checksum="E2CAB87186E3A365" modified="2011-11-16" version="1">MIELMTPKLWEYAKSEGNSVIVLNSKSTRALCAGGDVAACASGILNGDPHYGADFFYKEYNLNYMISTCPKPYISIMDGITFGGGVGLSVHAPFRIATERTQVAMPEMDIGFFPDVGATFFLPRMDDKLGYYIALTGTVLKGAEAYHSGFATHYIKSENIPKLTKALSKSYSLEIDSERFLQEINGILNNFSEKQYPEGYKFPLSVENLQVINKAFSQPSIPDVLSYLQQEGSTFATETFEKLSKKPMTSLAIAYELLNRGAKNSIKQQFELEMVAATNIVCIPPAQNDFANGVAHKLIKKIKDPFFPEWNPPSFVTKDFVQGILKPSANVDKYLPKPLISPWFGVDFDQYPHNMGLPSNAEVESYIKGSDGSDRAYLPTPNEVVRHFVNRSKHKLGVAEKVEQILNLHGETAKYDNKYVNWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFI9</accession>
    <name>G3AFI9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Globin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_131849</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372390.1">
        <property type="nucleotide sequence ID" value="XM_007372328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFI9"/>
    <dbReference type="GeneID" id="18869614"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131849"/>
    <dbReference type="eggNOG" id="KOG3378">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1016224_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFI9"/>
    <dbReference type="OrthoDB" id="436496at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008941">
        <property type="term" value="F:nitric oxide dioxygenase NAD(P)H activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071500">
        <property type="term" value="P:cellular response to nitrosative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046210">
        <property type="term" value="P:nitric oxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01040">
        <property type="entry name" value="Mb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044399">
        <property type="entry name" value="Mb-like_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396:SF6">
        <property type="entry name" value="ABL201WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396">
        <property type="entry name" value="FLAVOHEMOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Globin" evidence="1">
        <location>
            <begin position="65"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="274" mass="31283" checksum="655E9319845B6CC0" modified="2011-11-16" version="1">MELNQIKVSLSLSKSEIDIIRYSWQDMLIENETTFPRPTIRSTNPSIPGGYPEEYPNEPDNQTIAASVSDRVKAKSLARATTLFCQQLYKNLLDRDHALERMFPSIKHQATHFATVLTVAVMQLDDLSTLDDYLCRLGKRHARVLGVEPAQFELMGEALVETFHQRFGARFNQELEIVWIKLYLYLANTILQFGIDPVMKLEQEQLPAYDNRVYNQETNMGSEPDCPMEVWDVEAPPLLTPKIISVPSPTIYDDFDQTSMGRGYHSACPSYGNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFX3</accession>
    <name>G3AFX3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Oxidoreductase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_58265</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372524.1">
        <property type="nucleotide sequence ID" value="XM_007372462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFX3"/>
    <dbReference type="FunCoup" id="G3AFX3">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFX3"/>
    <dbReference type="GeneID" id="18872389"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58265"/>
    <dbReference type="eggNOG" id="KOG4690">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062297_1_1_1"/>
    <dbReference type="InParanoid" id="G3AFX3"/>
    <dbReference type="OMA" id="CVWEIYN"/>
    <dbReference type="OrthoDB" id="10064411at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019180">
        <property type="entry name" value="Oxidoreductase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039251">
        <property type="entry name" value="OXLD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21193">
        <property type="entry name" value="OXIDOREDUCTASE-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21193:SF3">
        <property type="entry name" value="OXIDOREDUCTASE-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09791">
        <property type="entry name" value="Oxidored-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase-like" evidence="2">
        <location>
            <begin position="109"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="239"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="254" mass="28129" checksum="8B3F932419FF605E" modified="2011-11-16" version="1">MSVGLRPAKRAVAAAAAQAPSFRASNHRYAFYDLILRTSSHPTHPIEASLMTSNELARLKARTKTTFEGNYYIDPSLTPEQRIAKVFGGRIKGEGRESSSRITRGEPKVIAGVTVPAKPDEPDNCCMSGCINCVWELFDEDIKDWNEKRKRAAKLLVKQGGRWPEDFDAPLKYLGKENLPVSLLNKPSLYEAKQKALASSDEEESWGNVPVAMRVFAEVEKKLKAKKLARISHSEPTLQPQESKQSQASSSSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG64</accession>
    <name>G3AG64_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein STU1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_132669</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLASP family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372615.1">
        <property type="nucleotide sequence ID" value="XM_007372553.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AG64">
        <property type="interactions" value="147"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG64"/>
    <dbReference type="GeneID" id="18869679"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132669"/>
    <dbReference type="eggNOG" id="ENOG502QT5T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_261691_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG64"/>
    <dbReference type="OMA" id="AKECYWC"/>
    <dbReference type="OrthoDB" id="46159at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060172">
        <property type="term" value="P:astral microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024395">
        <property type="entry name" value="CLASP_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF9">
        <property type="entry name" value="CLIP-ASSOCIATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12348">
        <property type="entry name" value="CLASP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TOG" evidence="8">
        <location>
            <begin position="283"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="234"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="515"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="612"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="930"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="244"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="515"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="548"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="563"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="628"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="931"/>
            <end position="952"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1290" mass="145422" checksum="4A01D69745B479F7" modified="2011-11-16" version="1">MSNPIIPADELFQIIASPEVDEITKLEHIQRLKSRIKKGFVDLSLVPKYFDSLAIAVDIPSPEIRTNSLSTLSYLIKRISVQDKRESLLKQQSTTVVPILISKLADPSSNIVVLARNALENYWMVASTEVEDLILKQAFINLNSTLAYETVIWINHIANSINPNFRLGKFIPTLVKILATGDQNGKIDHQIRGLLDTFYSKSHNKAQIQDLVRELQAQKVTIQIQGETFPHITKPESVAKTRARSQTPDNTVQAKPEPHTSNQPDFSNSLNLLLQKTNYELDQAIEPENINDSATLLREFNSYLPCFQGKETESNWKIREKNIIHMRSILRGNARDDYRHDLLICFKDLSNGIIKAISSLRTTLSMHGCQLLKECAMLLQSDFDAIAELVFPTLIKLCSSTKNITSNNANMCIASFYANISYSPKALQRIVTVTEERNYQPRSYTAIWLQVILLRYADNSTFRETHATHVVEIVNKVLTKLLRDANPNVRSVAKDCFWTYGRVFPDQSEKLLSKLDSNTTKALQRSRPSSRAGERGPVIPVTKPQRTSLRESIIEKKREMRSRSSLSGRSTPDISSTQPLPKPLKSVPTRLGAPLVRSTTEPIEPTSISLRVGSLRPANPPTVHKDPVSQTLRESTTRSHSFGSIPKVEPPKPVSNPFTYTSDQDPLVNFLSSQDTAVVVEGISLLKFAVTQEELPKQVRDSLKRISIKQPFLLKPLFMANDKTFKASAKLFSPSDFLRLCSLLFPEVDAKTVDLIISCTDVDTFYDSAISLLLNVADTSHFYESQDLIMQLVKHKTEIARSIMQILSISLGRAPIADVYFSKFLAALFDLVISFKMTDNYQLCCQLVRQLYATNNMKFLTAVEQVDQSTREEIEYIVGIDSTFNLQPLSKSIYDLTEIPFQKSTPMDFSPVKVSTDFTMIRPARNVFGIDSDKKRNSSKSPAKEPIDKSVSPDEIQFEPTRDPVSVSSSANPSAANLVENFASVQITDENSRNNSAKSSIEAIMEKIDPLKSISNKSKKIAIYEDNKIEEDRSTDEVSSPSFEDYHIAKIAGGGHILNAWSMEQFESCCKHLTELPIDSVKVMRLIGILGSLPGCDHEVKAYFNNQGKHLLEANIWVYFEHLQSLPYSDVINGLVLLKQNVKYNETIDMDKLFKVMESTCMDESPGGEIHCIWDSILQSIYSKERIEEIMLSYLETPDLNTNVVTVCFEYFTTVVVENHDLSSVQTQRLVHLLSNFLESSNVDLLRLATVCYGKLLVGVDAQSETKTSLNEVKMKLPFHQQKLIEYYMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG84</accession>
    <name>G3AG84_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA helicase</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_53535</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372635.1">
        <property type="nucleotide sequence ID" value="XM_007372573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG84"/>
    <dbReference type="FunCoup" id="G3AG84">
        <property type="interactions" value="544"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG84"/>
    <dbReference type="GeneID" id="18871791"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53535"/>
    <dbReference type="eggNOG" id="KOG1803">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001666_8_2_1"/>
    <dbReference type="InParanoid" id="G3AG84"/>
    <dbReference type="OMA" id="TIIHGPP"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033203">
        <property type="term" value="C:DNA helicase A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043601">
        <property type="term" value="C:nuclear replisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006301">
        <property type="term" value="P:DNA damage tolerance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000326">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048761">
        <property type="entry name" value="SMUBP-2_HCS1_1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF8">
        <property type="entry name" value="DNA-BINDING PROTEIN SMUBP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21138">
        <property type="entry name" value="SMUBP-2_HCS1_1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="229"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW35223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="685" mass="77461" checksum="F618EEED13EB9A74" modified="2011-11-16" version="1">MKLFEQFKNAIEDEQQEDISETDAYISMYSPKKLSSLGLAIINLQIINLRTGLGAKTIIELKLDPAFGDTDINTGTLRTGDIVKLSKMEKSTSTNAKKKKTKSTTSTENSEDSEDQGIEAVVLKVSTQTISISVEESTDDAHVLQYYNNTNNDSTRMWLVKLANSITYKRMISTMNKVNELQPSDKNDIHKLLLGESSYISHQNNHFITFFNSHLNDSQKQAIEFAINKSNITIIHGPPGTGKTYTLIELIQQLTNLGEKVLVCGPSNISVDTILERLGDKYKSGQLIRIGHPARLLPVNLQHSLEILSKSYGREIISDIEHDISSTLTKIRKCKRYAERRELYQELKLLKRELKQREKKIVHELLINARVVVATLHGSGSYELRTNDLAFDTIIIDEVSQSMEPQCWIPLLLNTKFKRLVIAGDNMQLPPTVKSVKRKGSSILETTLFDRLVLKGEGNKFKKLLDVQYRMNTSIMMFPNMQLYSNKLKSDSSVENITLSELPGVEENDDTLCKCIWYDTQGGEFPEQVSESVEGDSKYNEMELLVVRGHIEKLLSDGVQPKDIGVIAPYAAQVQLLKKQMGPETEIEISTVDGFQGREKEVIILTLVRSNESREIGFLSDQRRLNVAITRPKRQLCVIGDLELMSSSGSIFLKNWCKYVEEGIDCKDVEPFEIVYPNLEDYLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG93</accession>
    <name>G3AG93_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Eukaryotic translation initiation factor 2 subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_58437</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF2B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372644.1">
        <property type="nucleotide sequence ID" value="XM_007372582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG93"/>
    <dbReference type="FunCoup" id="G3AG93">
        <property type="interactions" value="1325"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG93"/>
    <dbReference type="GeneID" id="18872450"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58437"/>
    <dbReference type="eggNOG" id="KOG2916">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033458_0_1_1"/>
    <dbReference type="InParanoid" id="G3AG93"/>
    <dbReference type="OMA" id="DVNEHQR"/>
    <dbReference type="OrthoDB" id="1685042at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043614">
        <property type="term" value="C:multi-eIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990856">
        <property type="term" value="F:methionyl-initiator methionine tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1130:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000015">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.190:FF:000002">
        <property type="entry name" value="Translation initiation factor 2, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="entry name" value="Translation initiation factor 2, subunit 1, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="eIF-2-alpha, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="eIF2alpha middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="10">
        <location>
            <begin position="17"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW35232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="300" mass="33999" checksum="42EAA37A67F6D2F8" modified="2011-11-16" version="1">MSSSNCRFYENKYPEVDEVVMVNVQEIAEMGAYVKLLEYDNIEGMVLLSELSRRRIRSIQKLIRVGKNEVAVVLRVDKEKGYIDLSKRRVSAEDIVKCDERYNKSKAVHSILRHCAEKFNISLETLYETIGWPLSRKFGHAYDAFKISITDPTIFDDIQAPNPEVLNELKLYISRRLTPQAINIRADVEVSCFSYEGIDAIKEALKAAEAVSTPQMQVKAKLVAAPLYFISVQALDKNQGIKLLETAIEKVTEIIESKGGVCKVNMAPKAVTATEDAELERLLERKEAEDRESSDEEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGJ8</accession>
    <name>G3AGJ8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58569</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372749.1">
        <property type="nucleotide sequence ID" value="XM_007372687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGJ8"/>
    <dbReference type="STRING" id="619300.G3AGJ8"/>
    <dbReference type="GeneID" id="18872506"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58569"/>
    <dbReference type="eggNOG" id="ENOG502RF8N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020132_1_0_1"/>
    <dbReference type="InParanoid" id="G3AGJ8"/>
    <dbReference type="OrthoDB" id="2399539at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47431:SF1">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47431">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="517" mass="57593" checksum="61E8E5806532B7C7" modified="2011-11-16" version="1">MYAKQVDLISDRLIQCVNIVKQNNQLDVVSIQALLLVAIVAHVSSFHVFSKKLRHFCIHLIEDMKINTLDKHEFINPTTDALTPTSSIASAGSPGQSVNVLQSPRLSHVAAASVADSARRLFWELYFFDIIIGSADGKSISSLDALELDINYPTKPSQQEFDYKGRAEAGKLVTQAVKMNVELIEKKPYEATLTKLKASLSSWEMKLEDPKLFGSPPLIHKNGSVNEGVHQSILLYNYAKIFAHRPFSYLWKINSPQNPKCNEEVLEAKDVQTTMKADARTNIETKKTIEAANSIVEVLIDTNASKVLDRTPLFACGLALSSLVHLSAYIWVETTLANDSTRNFDLNESDLEIFTEYIKLSLTAIYPISRHWTLSGKLAKHIRESLTTLRPKLYSKLKDFLPQIEVGMEQMTIADSSSSQPSALNSTNAQHIISNDTTPGTTYTNTQAYEPQQQPVVTNYSPYYLNSFDSTTADSVDYNGGLDGLQMGQMSPMSDTGCDWIDKALIDFFDEDNIMAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGP9</accession>
    <name>G3AGP9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58603</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372794.1">
        <property type="nucleotide sequence ID" value="XM_007372732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGP9"/>
    <dbReference type="GeneID" id="18872526"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58603"/>
    <dbReference type="eggNOG" id="ENOG502T5JW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171955_1_0_1"/>
    <dbReference type="InParanoid" id="G3AGP9"/>
    <dbReference type="OrthoDB" id="4086878at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="63" mass="7290" checksum="59E63A35CBFBA0A6" modified="2011-11-16" version="1">MLVYHLIWAIKVVYYQPYGNLLNNLVYGPGTLVANVGISSKLIRYINAKLVEDKIEPDYKKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGT3</accession>
    <name>G3AGT3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="10">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_131561</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="10"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372828.1">
        <property type="nucleotide sequence ID" value="XM_007372766.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AGT3">
        <property type="interactions" value="690"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGT3"/>
    <dbReference type="GeneID" id="18869589"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131561"/>
    <dbReference type="eggNOG" id="KOG1140">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_238060_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGT3"/>
    <dbReference type="OMA" id="HAIMINI"/>
    <dbReference type="OrthoDB" id="26387at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19672">
        <property type="entry name" value="UBR-box_UBR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.30:FF:000002">
        <property type="entry name" value="Putative e3 ubiquitin-protein ligase ubr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003769">
        <property type="entry name" value="ClpS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044046">
        <property type="entry name" value="E3_ligase_UBR-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_bL12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055194">
        <property type="entry name" value="UBR1-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF26">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02617">
        <property type="entry name" value="ClpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18995">
        <property type="entry name" value="PRT6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22960">
        <property type="entry name" value="WHD_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="ClpS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="12">
        <location>
            <begin position="98"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="9">
        <location>
            <begin position="98"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1763"/>
            <end position="1791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1770"/>
            <end position="1783"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW35416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1791" mass="204435" checksum="9D9860DE50205D0D" modified="2011-11-16" version="1">MVVSGSDLQTQLRKFLVELPARADFEFTKATKHDLYRALYVAITNQGQHLTSLFPDISIDELDQLQSQQLQFPLSLEPPFKVFYNTLKQTKYTHLPDKACAKVFAENEAVYCCQDCGFDDTCVLCGYCFNPDDHVNHNIYIYKSTGENGGICDCGDEEAFVEKLHCKCQIPTEDEPETIQEDVSELAPYLRDTIAVCLDYILDVLNMSVLALPMVHDKIDSSGTITTKTYSDNAVLSPSKRYGDTEDVNSDEWLLILWNDEYHNLQEAIDSINAGTGVGDSKASEIAYKIDKEGFAILKRDPHFENVFGAKILVEQGGLIATVESSRDHFREEIVKYMFKWFEEILNFKGNVEFRELCKHVFCELLLAPSHEVSTSVDVEPFKSGSLSDHDYFINGFQYQGKFKDAGLTRINPDFVDALEHPISAILDANDDPSTISHSRLQYLLAYQIRLRKSIRMKLGSLLVPCLVSEPDTKMEFSKQFIEMYPMLLNMMALTDREEHLNLLADISSQIFTCPNSVQYILREDLLRNIIGPLAALIERTSTVYNEKTGYHSFYDIGINSQKKRAIKLAIKYAIGHLTHFASSGLAAGEIKAFMNDDTFRLYILLLRNFQSYLLMKRKYGDHVERDSDDFMLHLNFFFPVVLEVKQVVELQYSNPKLVENAEVLMKYLFARPIEFSQPGVVKFQVSKEQISFANPLHSLLSFMIEYHGINQFIPTLQSYSQKLVEIADISLRSVVLGSQIKVGFWIRNGLSATHQSKLYFSSKTCDVTYYRDFHIVQIAALFGNDPQKTLTNILDRWELLPWFLNQVSYSQTIYEERFVTISERFIVFIYNLITDRTFLTVETPTERARRMLRTAMAYRLCREGKSYTFLRYKIESNLPTEEIDKVLYSIADYQPPSSLTDSGLYRLKEAIYEELDPLSVYLDPSKFQVISESIIKNVARIRKIKEDDVIIQPRITRANYDLVDNNIGQFVKTQMFAKLIYKFLQVAIDTSDETYLPQLLHLIHAILLDDELIHGKEYLNPYFVDIPISDLLLTIVESKMSKYVIQKADYLVDLLVVKDKRVLESLVDCFGEDYIQSYKKRKSALFETEVERKKRLATERANRIMRKFSRQREQFLSKNKDLDADLDNDDKSEVEGHLRTCVLCGEQESVDSPFGILASSTKAATFWKIPTSKHDQVANAYKKWDKSIAYDESATTYGVGYHFNAENDVPFEFPVVSTCGHGIHYECYKRGSTGLTHYPCPLCHDLHDMFIPSYIPSATNRISDRFLHGEPQFTRYNMITHCIDATSKINNVVSSLINEEYLNTEQQFDAHRMVLSTVLTRIPDRTKYGTGNTKSESYFYLLLAYSELIANTIRMNEIATRINGEEAYSNFLSQFSGSTKTLLISLVQGRALAYAKRDSPALLGTEALFQFEAERFWDSDSILDSVFNDLVALFFQTGESLATLTTMGMTKLLAVSLYSILFSCANNPGYEQYFVSETDVNIPDKHLNLFKGFLFQVTEMMEIEVKPVDSQVVVGLYLGIEHLMGVFLRQVVILADLLTSQVQGENMYESQPQFANLQAAIEDQSRVDCTDALTASLSVPSFNQLINTLLTGDDSSFENKIFEVTFLSKIPKYFEKGILNLNSPGMVHLITLPHDYNASLLNASDSNTHDKSICLFCSTWISNSRQPSHMATCASHTGIFYTPSTNTLRLNVHIGHSRIPLDLEGPYLTVHGEVKPRRTKLKATLNEFRYLCLNKLWLNQGLYGFVTRNLFGARTVDDLLGNDGNTSPFEDDSEDMDEDDETEIRGGIFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGT9</accession>
    <name>G3AGT9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Prephenate dehydrogenase [NADP(+)]</fullName>
            <shortName evidence="9">PRDH</shortName>
            <ecNumber evidence="9">1.3.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_58638</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>prephenate + NADP(+) = 3-(4-hydroxyphenyl)pyruvate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:21640"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="ChEBI" id="CHEBI:36242"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8 9">Amino-acid biosynthesis; L-tyrosine biosynthesis; (4-hydroxyphenyl)pyruvate from prephenate (NADP(+) route): step 1/1.</text>
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    <comment type="similarity">
        <text evidence="1 9">Belongs to the prephenate/arogenate dehydrogenase family.</text>
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        <property type="reaction ID" value="UER00962"/>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
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        <property type="entry name" value="Preph/Arog_dehydrog"/>
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        <property type="entry name" value="Prephenate_DH_fun"/>
    </dbReference>
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        <property type="entry name" value="PREPHENATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21363:SF0">
        <property type="entry name" value="PREPHENATE DEHYDROGENASE [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDH_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
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    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0521">NADP</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="10">
        <location>
            <begin position="16"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007964"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022498"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00051295"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00060605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
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            <dbReference type="PROSITE" id="PS51176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW35421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="447" mass="50780" checksum="462D6B557085B0C7" modified="2011-11-16" version="1">MDQLTTEQIDQLKQTKTIGIIGLGDMGTLYARRFSKAGWKVVGCDREELYEETLAKFSQEKFTILKNGHLVSRISDFIIYSIEAANIDKMVALYGPSSKFGAIIGGQTSCKAPEIAAFEKYLPPDTEIISVHSLHGPKVDPTGQPLVLIQHRARDESFQFVECLMSCLQSKHVKLTAKEHDRITADTQAVTHAAFLSMGVAWKSTNEYPWETPKWIGGIENAKINISLRIFSNKWHVYAGLAITNPSAHDQVLQYSKSTTELFTLMIQGKHAELKERLMKVKQFVFGGNNVASKHDLLLNDDLLQEFSLRKVPPEGRQPNSHLSLLAIADSWYNLGIVPYDHIICSTPLFRIFLGVTEFIFCTPNMLDECIDVAINDTSFRQDDLNFTIAAQTWSNIISFGNFELYKREFVETQNYFRHMFQEANEIGNEMIHTILERVKQREQEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGU1</accession>
    <name>G3AGU1_SPAPN</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_58641</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007372774.1"/>
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    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58641"/>
    <dbReference type="eggNOG" id="ENOG502RRZT">
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040410">
        <property type="entry name" value="UPF0658_Golgi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34391">
        <property type="entry name" value="UPF0658 GOLGI APPARATUS MEMBRANE PROTEIN C1952.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34391:SF1">
        <property type="entry name" value="UPF0658 GOLGI APPARATUS MEMBRANE PROTEIN C1952.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="118"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="230"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
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            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="339"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="441"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="463" mass="52768" checksum="F1B45E11D678E3FE" modified="2011-11-16" version="1">MNNYNNIGGLPPQPTQPPYPHSPYMSDRQPQHSLQQPFYDQQAGAYGTSNPYPSQEPTLQPEIPYTSHSYDNSDSESPPESPILHHVASNSSNISDIADFNRLNRKQSSELRTDERQYGLFSTFFFFISLVSAILIILFESYMFAVINIHHVDLGDGRYVEVSIYFALFIFAGIFQVLITIIGLKTRNMLLLFFLCLFYCCMLIYSGIQYNEVARRITVVLRGRWYKATFALNIATIAVIGATLLLQSIILIVALRKYVNWFTFKKVGADITLRRMFTVFQIHRSILMFDFFFFVGFTIQFVVIMVNDRTSVEFILTIVVLPCTIILLLISDISTCREFIFGSLFAIVLYLAGMAYVLFKIIRLYTHYTSAYGFAIEAGEYFMGRKSLTVFGVLTLVLLLITVVLECMVIYNFKKGLLPTVSTYYKWIPGYKKEAASSGINISDDDSEAKNQRTDSASDGYID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH29</accession>
    <name>G3AH29_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MAGE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_58693</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372871.1">
        <property type="nucleotide sequence ID" value="XM_007372809.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH29"/>
    <dbReference type="FunCoup" id="G3AH29">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AH29"/>
    <dbReference type="GeneID" id="18872566"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58693"/>
    <dbReference type="eggNOG" id="KOG4562">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049350_0_0_1"/>
    <dbReference type="InParanoid" id="G3AH29"/>
    <dbReference type="OMA" id="KFFLPKY"/>
    <dbReference type="OrthoDB" id="205198at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1200">
        <property type="entry name" value="MAGE homology domain, winged helix WH1 motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1210">
        <property type="entry name" value="MAGE homology domain, winged helix WH2 motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037445">
        <property type="entry name" value="MAGE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041898">
        <property type="entry name" value="MAGE_WH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041899">
        <property type="entry name" value="MAGE_WH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002190">
        <property type="entry name" value="MHD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11736">
        <property type="entry name" value="MELANOMA-ASSOCIATED ANTIGEN MAGE ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11736:SF14">
        <property type="entry name" value="NSE3 HOMOLOG, SMC5-SMC6 COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01454">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01373">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MAGE" evidence="2">
        <location>
            <begin position="44"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="319" mass="36430" checksum="F7E2F0DB72560361" modified="2011-11-16" version="1">MPKRRREIDDSDDSVEYDEGDRSMPNRSETGTPSHELQHIVNKVIRLVLTRESKGQLIRREHINQIINDRKNRSYDVVLKEVTVALEEAYGLTLVETPIIKKKGDTSKAKKVLKPKQPYAVVNCLTPEARNLLGDIWEKSLGTFTKNDDLGQSKFYVPRYSKSKLPTSNYELVKNGILLVIVSLIILSENHISESELFRNLRKFGISTNLNVKNSSLNMNIQELLNDLIRREYLNKNITKGRIESENVVEYTLGRRSLIEFPPQSCFEYIKKIFGENFDTGMAERTLVTIERAYGVALVNESEAVTPDVSRGQTPAEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH54</accession>
    <name>G3AH54_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_48473</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372896.1">
        <property type="nucleotide sequence ID" value="XM_007372834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH54"/>
    <dbReference type="FunCoup" id="G3AH54">
        <property type="interactions" value="167"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AH54"/>
    <dbReference type="MEROPS" id="C19.088"/>
    <dbReference type="GeneID" id="18871228"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48473"/>
    <dbReference type="eggNOG" id="KOG1870">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016013_1_0_1"/>
    <dbReference type="InParanoid" id="G3AH54"/>
    <dbReference type="OMA" id="WCTYDDE"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033567">
        <property type="term" value="P:DNA replication, Okazaki fragment processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000146">
        <property type="entry name" value="Ubiquitin-specific protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF758">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="305"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="42"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="94"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="136"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="175"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="214"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="262"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="275"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="643" mass="73259" checksum="082085B8F01125A2" modified="2011-11-16" version="1">MTPSISTANPASSPKSATSSPTKPKSFQEAVAAYTGKKFLTKQEKKRKRKLQLQQIQKQQQEQKEDKVEEPPVKKQKASTTSSILSNIFSLYKRVPFGGTPTPPPAPATETDEDLGEDLSESPFTGFSEAETKSQEEDDEGVDEEEKDEEQSNAVSEIDDEDDEEEDDDFKQTTSEESSPVDSSSPSSEEAIDEEEDMERLKHDLKVDQLINTGDDEEEDEDEDEDEDEEEEEEDEDDESSESVPAVKSISTPPTSPEEETTEKSIQKQEIEYNDINQDPNDRGSNNSTRIYKNWRELAKRKPPVGLLNQGVTCYMNSAIQSLLHIPAVQHYLNDISQGKYNNVLKPRSVSHTLAELSRKMWGYDKRPVKYVNPRKIISRLDDINCMMSEWQQEDSHEYFMSLMSRLQEDSTPKGKKLQESIIYDIFGGLLHQRITCSKCNNVSETKQEFYDLSLGLNKRKSKGDPSSKYSIEKSIREFFSNELIKVDSNLKDSGYFCETCHSHTQANKVSVINRAPETLTIHLKRFKFNGNSSSKVKQSIRYSKYLDLDQYTEGDITAKYRLVSVIVHEGRSISSGHYVAHCLQPDGVTWCTYDDEYINRIEERVALSDPSAYVLVYTKLTPKSKKRKFEEANGTPVKKRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHC0</accession>
    <name>G3AHC0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_59507</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373668.1">
        <property type="nucleotide sequence ID" value="XM_007373606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHC0"/>
    <dbReference type="FunCoup" id="G3AHC0">
        <property type="interactions" value="596"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHC0"/>
    <dbReference type="GeneID" id="18872947"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59507"/>
    <dbReference type="eggNOG" id="KOG1508">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072852_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHC0"/>
    <dbReference type="OMA" id="PGKEFPN"/>
    <dbReference type="OrthoDB" id="19419at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070775">
        <property type="term" value="C:H3 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010698">
        <property type="term" value="F:acetyltransferase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="207" mass="24466" checksum="E37F3E54EAFE795B" modified="2011-11-16" version="1" fragment="single">MTSREELSNEELSAKLTELSEWENQMSHTEKQLEITRLKKVHPLYEKRREILKSIPKFWYIVLAEGDEFVEYVNLDDLKYIEFIDDIYVYYPIADTDVSEDNFKDFSITISFAESELIPKQTVTKHFKTIFGEEGEKITSEPVDIIWPTDIEDLNPRAIKQKNKGKQLSTEDKKNYRAGMRSFFAWFQWTGEKPGKEFRNGEDLTNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHI1</accession>
    <name>G3AHI1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nucleolar complex protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_54330</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373729.1">
        <property type="nucleotide sequence ID" value="XM_007373667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHI1"/>
    <dbReference type="FunCoup" id="G3AHI1">
        <property type="interactions" value="1090"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHI1"/>
    <dbReference type="GeneID" id="18871852"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54330"/>
    <dbReference type="eggNOG" id="KOG2256">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011272_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHI1"/>
    <dbReference type="OMA" id="GCLRYYL"/>
    <dbReference type="OrthoDB" id="10266662at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030690">
        <property type="term" value="C:Noc1p-Noc2p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030691">
        <property type="term" value="C:Noc2p-Noc3p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005343">
        <property type="entry name" value="Noc2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12687">
        <property type="entry name" value="NUCLEOLAR COMPLEX 2 AND RAD4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12687:SF4">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03715">
        <property type="entry name" value="Noc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="71"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="141"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="675"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="88"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="104"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="141"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="675"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="688"/>
            <end position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="709" mass="81708" checksum="58678D6B58B63B82" modified="2011-11-16" version="1">MAKVSKQTKKFQNKHLKHTIEQRKKVQSHSKKIALRKKGKSTSEEASEPKRKDGKAKEVFEDMSVDDFFAGGFEVPKEKKTKKKKVEESDDEESSEEEEDEEVMKENLKNLEEQDPEFYKYLKENDNELLDFEGVNPLDAMDEDESEEEDEEEEEVKEKTKKKADTATAPGKVEITLELVKKWEQQLEKPSPKLLRNITIAFKAAVNINNSDEEDYKYSVTDPNAFSELMFVVLKKVPVAIQKLVKYKTNSQGVRTLPQKNQNVTQISSILKSHAGSFITLLKDITNTEIAALVLSSIYELIPYYLSYRRLLKEILSSVVDVWSTTPDIQTQIATFAFLNNVAKEYPKSVLENILKLSYSGMLQNCRKTNIHSMSQINFCKNSASELYGIDETLSYQVGFEYIRQLAIHLRNSINATSNAKEGYKTIYNWQYCHSLDFWSRVLSQHCNPEIELQNHKSKESPLRQLIYPLVQVTLGAIRLIPTAQFFPLRFYLIRSLLRLSQSTGVYIPIFPLISEILTSTAMTKAPKGSSLQAFDFDHNIKANQAYLGTRVFQDGLCEQFIELTSEFFGLYAKSIAFPELVTPAILSLRRFTKKSKNIRFNKQLHQLVEKLNANATFITGKRSNVEYGPSNKTEVQLFLNDFDWEKTPLGQYVIVQRQTKDEKMRILKEALEEEEKAKAEQKKKDEQDDDIEIDVDSEEEDSEEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHN1</accession>
    <name>G3AHN1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_54369</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endomembrane system</location>
            <topology evidence="5">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373779.1">
        <property type="nucleotide sequence ID" value="XM_007373717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHN1"/>
    <dbReference type="FunCoup" id="G3AHN1">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHN1"/>
    <dbReference type="GeneID" id="18871857"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54369"/>
    <dbReference type="eggNOG" id="KOG0724">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037236_2_0_1"/>
    <dbReference type="InParanoid" id="G3AHN1"/>
    <dbReference type="OMA" id="RRYDYFY"/>
    <dbReference type="OrthoDB" id="413400at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052606">
        <property type="entry name" value="DnaJ_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44653">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44653:SF2">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442517" description="J domain-containing protein" evidence="8">
        <location>
            <begin position="20"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="9">
        <location>
            <begin position="45"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="310" mass="36431" checksum="698E9325A153FAF5" modified="2011-11-16" version="1">MKIFNLHLTFLFLVSLVVASNWSKEDYEIFSLNDKIQQDLGKETTFYSWLDLERGPKATRDEINKAYRKLSRKLHPDKYSKKSRKEKKNAEDRFQRLSLVGNILRDKSLKRRYDYFYDKGFPKWKGTGYYYSKFRPGVVFTFAILYIIVGTFQYISLRINRKQDYKRILDLKNELINQAWGGSPFPPAGITDRKIVNQANEKEFVVTPTGNVFLVDAETKELIELDENDINLKPGFKESMFYKIPVCIYNLSVGKWISKPIDTTITYVNPKRAETQEAERVEKKKARKKAQRGDKKIELPNGKVVYSRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHX0</accession>
    <name>G3AHX0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_59702</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373868.1">
        <property type="nucleotide sequence ID" value="XM_007373806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHX0"/>
    <dbReference type="FunCoup" id="G3AHX0">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="GeneID" id="18873037"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59702"/>
    <dbReference type="eggNOG" id="ENOG502S2HK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148825_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHX0"/>
    <dbReference type="OMA" id="EGWLWNK"/>
    <dbReference type="OrthoDB" id="4083608at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053092">
        <property type="entry name" value="Mitochondrial_unc_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28048">
        <property type="entry name" value="ACR195WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28048:SF1">
        <property type="entry name" value="ACR195WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="97" mass="10645" checksum="86991734D3CB7E27" modified="2011-11-16" version="1">MSNILSVFNPPPSRDLSDAELGKACLPCTAIQSFVAMAGGAYLNSPNQFKDKAGKIDFVKHPLWWQRSVRGAGIVLFGFGAYRAGEVIQILLEDRFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHY9</accession>
    <name>G3AHY9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_59726</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373887.1">
        <property type="nucleotide sequence ID" value="XM_007373825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHY9"/>
    <dbReference type="FunCoup" id="G3AHY9">
        <property type="interactions" value="607"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHY9"/>
    <dbReference type="GeneID" id="18873047"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59726"/>
    <dbReference type="eggNOG" id="KOG0737">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_21_14_1"/>
    <dbReference type="InParanoid" id="G3AHY9"/>
    <dbReference type="OMA" id="REPRIGC"/>
    <dbReference type="OrthoDB" id="10254455at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140567">
        <property type="term" value="F:membrane protein dislocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140570">
        <property type="term" value="P:extraction of mislocalized protein from mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034214">
        <property type="term" value="P:protein hexamerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000538">
        <property type="entry name" value="ATPase family AAA domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051701">
        <property type="entry name" value="Mito_OM_Translocase_MSP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644">
        <property type="entry name" value="AAA ATPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G12920)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644:SF3">
        <property type="entry name" value="FI08533P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="126"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="247" mass="27383" checksum="0F9A86417FCD9654" modified="2011-11-16" version="1" fragment="single">MGKFKVDLKLISDLFILAGAGLSVYYIINTLINDYIDSPSIKNKENEKKGSGILKKIQSSNPHLKDISLNQYEKSLLNCLVIPDDISVSFNDVGGLQDIIDEVREAVILPLTEPELFATHSSLIQSPKGVLFYGPPGCGKTMLAKAIAKESGAFFISIRMSTIMDKWYGESNKIVDAIFSLANKLQPCIIFIDEIDSFLRDRSSNDHEVSALLKAEFMTLWDGLKSNGRIMVLGATNRKNDIDEAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI98</accession>
    <name>G3AI98_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Low temperature viability protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_53981</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LTV1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373251.1">
        <property type="nucleotide sequence ID" value="XM_007373189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI98"/>
    <dbReference type="FunCoup" id="G3AI98">
        <property type="interactions" value="488"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI98"/>
    <dbReference type="GeneID" id="18871827"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53981"/>
    <dbReference type="eggNOG" id="KOG2637">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028555_1_0_1"/>
    <dbReference type="InParanoid" id="G3AI98"/>
    <dbReference type="OMA" id="TAQHFTL"/>
    <dbReference type="OrthoDB" id="5852896at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071986">
        <property type="term" value="C:Ragulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904669">
        <property type="term" value="P:ATP export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006970">
        <property type="term" value="P:response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007307">
        <property type="entry name" value="Ltv1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531">
        <property type="entry name" value="LOW-TEMPERATURE VIABILITY PROTEIN LTV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531:SF0">
        <property type="entry name" value="PROTEIN LTV1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04180">
        <property type="entry name" value="LTV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="125"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="258"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="382"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="127"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="296"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="423" mass="49480" checksum="C1872954F55E99A8" modified="2011-11-16" version="1">MAPRRKFDKKNATTFNIVHRAHDDAKFYDEEASEHVLVPAASKQPQQTKKKIYTPNELKEKLASDEIRENEGMAAQYGIFYDDSKYDYMQHLKPMGQQDSVFIGVDEKPKKETKIEDLFKDQLPSEQTRKIGHDLNESIPKELQGFNPNLDPRLREVLEALEDEAYIEGEEEEGEGDIFNDLLQSGEVEDEQEFYYGSDAEAYDEDYDEWDLDNYEEEYNKKYEQGEPKEYNHDEITDEVPEIHQNWQGVMQFKKENKNKVNDWDSDDDFEDEDEQDEEEEEYDALGDLPKIDNKKKSKTKLRKKKGAMTDTSSFSMSSSALFRTEGLTLLDDRYEQLNKKFEEDPQEEEEYSPFNMEEERGDFESMLDDFLDNYELESGGRKLAKKDEEKKKLQNAADSVSRGKVAKARMKGLSSSFDNMKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AID1</accession>
    <name>G3AID1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_59835</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373985.1">
        <property type="nucleotide sequence ID" value="XM_007373923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AID1"/>
    <dbReference type="FunCoup" id="G3AID1">
        <property type="interactions" value="129"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AID1"/>
    <dbReference type="GeneID" id="18873095"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59835"/>
    <dbReference type="eggNOG" id="ENOG502RDJ2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_505248_0_0_1"/>
    <dbReference type="InParanoid" id="G3AID1"/>
    <dbReference type="OMA" id="MIYLEMT"/>
    <dbReference type="OrthoDB" id="270970at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08681">
        <property type="entry name" value="C2_fungal_Inn1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037791">
        <property type="entry name" value="C2_fungal_Inn1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46502:SF2">
        <property type="entry name" value="16 KDA PHLOEM PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46502">
        <property type="entry name" value="C2 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="4">
        <location>
            <begin position="9"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="268"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="202"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="278"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="331"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="522" mass="58620" checksum="B52CC809094A5039" modified="2011-11-16" version="1" fragment="single">MNSLSMNSIPSLRNDSPTIYPESADGTLVIMVVRAKHLPNRRKLDKQSPYVTVRLGTTAKKTPAAFRGGQVPEWTHEIRFPLTRDRKPILKLDVLDETKGDPTPIGNAEIDCSVLFTREEENGKYIYDNWFELSQGGKRAGMVYLEMTFYPSAPVLPSKIPIYPHDQVVTQEENYDVHDSYHEQPVYHRPQVPHKEDVFVNSESSTSSKGSSTFSHNTGKHYNESTGTVNSASAVFVNEQQYQENLKGAKKYALKFAKLKSKFNTREPLNTLWGGNESSTLSNHLSSSPSKRVVSPLSEYGSDDNSPYSHHEEDIQFISPPEPPRRDFKIVPPPRSPTPPPKSPSKSPLSESRRSPRRKPPADIPFEVGKLKDSTSIPFSADTIGLEDSEGLPTKVYLLDQPVKSLSYSADNIISPVKLKGDEIDPRYYAPTPSEHFNRNSRLTAGSATKDDLKVDLNTSETGYIGNGKFSPSVFQRIGRNYWSSESELDQQDKPKVPPKIPEGLTEAEYYVLEKEKFLRDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIG4</accession>
    <name>G3AIG4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Membrane magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_49477</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the membrane magnesium transporter (TC 1.A.67) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374018.1">
        <property type="nucleotide sequence ID" value="XM_007373956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIG4"/>
    <dbReference type="FunCoup" id="G3AIG4">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIG4"/>
    <dbReference type="GeneID" id="18871343"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49477"/>
    <dbReference type="eggNOG" id="ENOG502S8V0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132206_0_1_1"/>
    <dbReference type="InParanoid" id="G3AIG4"/>
    <dbReference type="OMA" id="STHYHTD"/>
    <dbReference type="OrthoDB" id="44756at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053279">
        <property type="entry name" value="EMC_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018937">
        <property type="entry name" value="MMgT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28144">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28144:SF1">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10270">
        <property type="entry name" value="MMgT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="13891" checksum="ED0EB2310B6544BC" modified="2011-11-16" version="1">MLFYAIGILLLTHAGYSAFEHHKLASHTIHSALPLDIIVETIVATVIIVFGSITSIKNSPALTLDNEVVTMDSQYLKPIRATDASQLDQIVGVSEYDRFDNRIEFVDVIAKRREYAEWIKEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIZ5</accession>
    <name>G3AIZ5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_59942</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the nudE family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374091.1">
        <property type="nucleotide sequence ID" value="XM_007374029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIZ5"/>
    <dbReference type="STRING" id="619300.G3AIZ5"/>
    <dbReference type="GeneID" id="18873143"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59942"/>
    <dbReference type="eggNOG" id="KOG1853">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1528821_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIZ5"/>
    <dbReference type="OMA" id="HMVETHQ"/>
    <dbReference type="OrthoDB" id="5877028at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051642">
        <property type="term" value="P:centrosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033494">
        <property type="entry name" value="NUDE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10921:SF1">
        <property type="entry name" value="NUCLEAR DISTRIBUTION PROTEIN NUDE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10921">
        <property type="entry name" value="NUCLEAR DISTRIBUTION PROTEIN NUDE HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="18"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="176" mass="20892" checksum="17A0E77BCB95207B" modified="2011-11-16" version="1" fragment="single">MDRHKLSELSRDDLISKVVELESILNDFQQSSKELERALEDELQELETRNNKLKRDITVLQDHLTQEKRKNIKLNAEINDIQESTSGKIKSLEEEVRGLSQKLVRIEIVNDSMESNDRILCNKYEISQEFSNELLEKVALLEDEYDRERKLNLERQLHITNYQNQINDLKVEIRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ16</accession>
    <name>G3AJ16_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">SPAPADRAFT_134241</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374112.1">
        <property type="nucleotide sequence ID" value="XM_007374050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ16"/>
    <dbReference type="FunCoup" id="G3AJ16">
        <property type="interactions" value="160"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ16"/>
    <dbReference type="GeneID" id="18869794"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134241"/>
    <dbReference type="eggNOG" id="KOG0519">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003731_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ16"/>
    <dbReference type="OMA" id="WGDSNRI"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005034">
        <property type="term" value="F:osmosensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036180">
        <property type="term" value="P:filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007234">
        <property type="term" value="P:osmosensory signaling via phosphorelay pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900445">
        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000004">
        <property type="entry name" value="Ethylene receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000289">
        <property type="entry name" value="Osmosensing histidine protein kinase SLN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047:SF72">
        <property type="entry name" value="OSMOSENSING HISTIDINE PROTEIN KINASE SLN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="349"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="19">
        <location>
            <begin position="444"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="17">
        <location>
            <begin position="511"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="18">
        <location>
            <begin position="1024"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="392"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="675"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="732"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="956"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1159"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="395"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="422"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="675"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="732"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="749"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="762"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="786"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="992"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1174"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="14">
        <location>
            <position position="1079"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="1193" mass="132450" checksum="C99506F7A78A8814" modified="2011-11-16" version="1">MRRLKIGIRPQLIVLVCFASLFSLLILAIVTGVYFSTNLNNLRKERLEVIAQLKATQVQQSIIYISYQVLWLTNVEAITTPLSQYKAGNNSVEIFSRAQQTLDQLMASTELFAAAKLYNLDLEVMASSYSNSTIVSDPTLSALFPLHPNATIPSTLLNPQADSDSSVYFTGPIANNSHELTSTYFMGITLPVYSNSSIILDQPSVSGYLSVIASAQNIQLALNANASGQPDYQAIAVKPIYGTLVSGIDNNSDNAQNDTSVQPIGFETVFPVSNSLLEPGNIYNINSSSSIKVAIESNTGSNTNVRSYSGNRVAIGFTPISIDNDTKWIIIVVQQRSVFMEPINTLKRIIIGVVIGIGAFMCLITFPLAVWFIRPITKLKLATEAITRYKKEKGNNGGGGGADGSGNDYESIPLASKKHTSRSNSINSTQTGTTNSVYSTGILLPARIPRSKKFFKDELTELSEAFNIMTEELDKQYTHLEDRVKLRTKELEASKIEAEAANEAKTVFIANISHELRTPLNGILGMTSIAMEEEDQTRIQDSLKLIHRSGELLLHILTELLTYSKNTLNRSKLEKSNFQILEIVYQIKSIFNKLAQDQRVNFKIFVKPNFFRKLIIYGDSNRIIQIVMNLVSNSLKFTPVDGSVDVTFKILGEYDLERSQRENFTKVYIMNETPSNESTTSISSKRGIFKRRKKEAEQKEDILNQDDVMSIITLSTQEYENKIFESQFNNKSLPEIPIPSSSSSNEGEVPSKDSSYIDEKQQSVSSGTDSTHSITDEKGGELAPITSPTSSQRQQINIIPQPEFKGYPTFEKKPLVDPTNISNNEIIKNNKTYRMRKLYNPKTWVIQIDVTDTGPGIEPALQEKVFEPFVQGDQTLSRSYGGTGLGLSICKQLATMMKGTLTLKSTIGQGSTFTLTIPIPQTGEIIVDPEDLNDFCDDEFNPNAKVNRRVVFEDDENLESNRDSESQDEVASSPLKFANSNKSFESKPPLYTRSSTGTANSGGDSKKSTSDHNSTLLDEISHLKILVAEDNLVNQEVIRRMLKLEGFNNIAMACNGAEAISLVKESIEQDDVFDLIFMDIQMPKIDGLLATKMIRNNLHYNNPIIALTAFADESNVRECLNNGMSGFLSKPIRRTNLRKIIVEFSPVLLNETVITPQTHHSNEEKRLGYGTSYIPPNNNSHDTTMSPSIDQLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ19</accession>
    <name>G3AJ19_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Dipeptidyl aminopeptidase B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_133435</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S9B family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374115.1">
        <property type="nucleotide sequence ID" value="XM_007374053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ19"/>
    <dbReference type="FunCoup" id="G3AJ19">
        <property type="interactions" value="315"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ19"/>
    <dbReference type="MEROPS" id="S09.006"/>
    <dbReference type="GeneID" id="18869748"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133435"/>
    <dbReference type="eggNOG" id="KOG2100">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006105_0_1_1"/>
    <dbReference type="InParanoid" id="G3AJ19"/>
    <dbReference type="OMA" id="MRTPQEN"/>
    <dbReference type="OrthoDB" id="16520at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002471">
        <property type="entry name" value="Pept_S9_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF200">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 10, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00708">
        <property type="entry name" value="PRO_ENDOPEP_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="15">
        <location>
            <begin position="157"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="14">
        <location>
            <begin position="606"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW34531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="821" mass="94408" checksum="1A491C8902BA9DBC" modified="2011-11-16" version="1">MGYIELPGSGESVSRTRPPFRSYFYFGIILGILVYGASFLLITIQDFQLNVSILQTDKPSHYASPSRRSVTGKKIPFSNEIYNSRVLAPKYTSIQWIREPGSIYNDKGAYVIKQEKDDGFEIVVKSMDDEKYSYILIDKSTFKHNHMEYDIADYVASPDLKKVVLKTNVTGSFRHSSFALYWILDVDSKRIRPVYNENDLISTVSWSPDSTNIAFIYNNNIYLKNIESDSLKQVTTDGSVQLFNGKPDWVYEEEVFGRDIVLWWSPKGDKFTFLRSNNTAVPEFTIPYYTHDKFEDYPEISKIKYPKAGYPNPIVDLLTYDLAGDKLAQHELKSHTIKPKERLITEVVWIGESILVKTTNRASNKQEIYLVDGDNVDLIRKSNAKNSWFEITSNTIYVPKNKTLGRKYDGYIDTVTEGGYNHLAYFSPPENPKGQLLTKGNWEVVNGVEAYDYTNNQVYFTSTKKSSIERHVHSVNLLDRTNDGLPYIKDITTKEGWYDVSFSAGARFLYLSDRGPGVPTQRINDLRMNKHVQTIEDNSELVKKLAKYDIPKVKYETVELTDESGETFLVNAMETLPLKFDPKKKYPVLFFIYGGPGSQQVSKRYSLSFSSVVAAELDAIVVSVDGRGTGYNNLNFKLGSDFKFIVRDQLGTYESKDMISAARLWSTRSYVDPERIAIWGWSYGGYLTLKTLESDIEDPVFSYGVAIAPVTDWKLYDTIYTERYMDTPENNHNGYITSSIHNYTNFAHVKKFFIGHGSGDDNVHLQHSLKLIDEFNLHDVENFEFMIFPDSNHSINYHNGNRIVYDRILEFFRKAFDWKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJA3</accession>
    <name>G3AJA3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_49597</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the exportin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374146.1">
        <property type="nucleotide sequence ID" value="XM_007374084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJA3"/>
    <dbReference type="FunCoup" id="G3AJA3">
        <property type="interactions" value="1485"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJA3"/>
    <dbReference type="GeneID" id="18871354"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49597"/>
    <dbReference type="eggNOG" id="KOG2020">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011906_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJA3"/>
    <dbReference type="OMA" id="WAFKHNN"/>
    <dbReference type="OrthoDB" id="27218at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030621">
        <property type="term" value="F:U4 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071528">
        <property type="term" value="P:tRNA re-export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000490">
        <property type="entry name" value="CRM1p Major karyopherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041123">
        <property type="entry name" value="CRM1_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041235">
        <property type="entry name" value="Exp1_repeat_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014877">
        <property type="entry name" value="XPO1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040485">
        <property type="entry name" value="XPO1_repeat_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF2">
        <property type="entry name" value="EXPORTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08767">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18777">
        <property type="entry name" value="CRM1_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18784">
        <property type="entry name" value="CRM1_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18787">
        <property type="entry name" value="CRM1_repeat_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01102">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="6">
        <location>
            <begin position="34"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1083" mass="124044" checksum="D9A89A45F07469ED" modified="2011-11-16" version="1">MEAILDFSKDLDISLFDQVVDTFYKGSGENQQKAQLVLNQFQEHPDSWTLSDKILSNSNNSQSKYIALSCLNKLIQYRWKTVAEAERVGIRNFIVNMIISLCENETIFETQRALINKIDLTLVSVLKQEWPHNWPKFIPEIVLSSRSSFNVCENNMIILKLLSEEVFDYSQDQLTQAKAQQLKTSMKNEFEEIFKLCYEVLDKTTKPSLIIATLNALLKYIQWIPTNYIYQTNLLDLLTNKFLAPADTRAIALKCLTEISSLELVDGNEEKTLIYFKNTMEQIYSIIPVSTNLKETYKVANSSDQSFLQDLAMFLCTFLSNNLILLEKLPDTQELLQNALYYLLELSRIEERELFKTCLDFWSGFVSELFKEIRKLPSNELSPMMQLTYGNSLRPTSSGGAPDPAVLAKFPLRQHKYAEVLSKLRLVIIENMARPEEVLIVENDEGEIVREFVKESDTIQLYKSMREVLVFLTHLNVVDTETIMIEKLARQIDESEWSWQNINTLCWAIGSISGAMNEDMEKRFLVSIIKDLLSLTEMKRGKDNKAVVASNIMYIVGQYPRFLKAHWKFLKTVVNKLFEFMHETHEGVQDMACDTFIKITLKCKKHFVVTQSGEREPFINEIIRNIQSITEDLQPQQVHTFYEACGIIVSAQSQKDARDKLLGELMALPNMAWQAIIQQAGQDPQLLTNTDTVKIIANIIKTNVSVCKALGPGFYSQLGTLYVDMLSLYKAVSSMISDAVAKDGIIATKTPKVRGLRTIKKEILKMIETYINQADNLEEIVRDLAQPLFAAVLEDYSSNVPDARDAEVLRTLTALVSKVGRLIPDGVVLILQNVFECTLDMIKNDFVEYPEHRVEFYKLLKEINSKSFQGLLQLSGDAFQSLINAALWALKHNNREVEDNGLSLTLDLVENVEQLGDTPFTKAFYENFYFQILSDTFYVFTQPDHKAGFRYQSQLLAQLIHLVQDNVIKVPLYTPDQAPEGTSNSEFLKQYLSQLLSSAFDNLQKEQLVNFLNVLTSVYNDLPRFKGILRDFLVQLKEFGGDPTDYLFAEDKELEEKEKVKLQRQQDMLVGGLIRPSEMVDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJK9</accession>
    <name>G3AJK9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Secretory lipase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_149745</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:12045"/>
        </physiologicalReaction>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374198.1">
        <property type="nucleotide sequence ID" value="XM_007374136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJK9"/>
    <dbReference type="GeneID" id="18870840"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_149745"/>
    <dbReference type="eggNOG" id="ENOG502S2P7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029538_5_0_1"/>
    <dbReference type="InParanoid" id="G3AJK9"/>
    <dbReference type="OMA" id="RQYCATG"/>
    <dbReference type="OrthoDB" id="2373480at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005152">
        <property type="entry name" value="Lipase_secreted"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34853">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34853:SF1">
        <property type="entry name" value="LIPASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03583">
        <property type="entry name" value="LIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442553" evidence="4">
        <location>
            <begin position="18"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="467" mass="52219" checksum="BCD2D01924EF7E24" modified="2011-11-16" version="1">MNILWFIVIVWRYLLQADITIANPHYDDFYTLPEGFDDAKPGDILKYREVPPKLASLFFPVEVKKVWQLVICSQDTFGNPTAFLSTIIEPYNADPSKVVSYQTFEDSANLTCSPSFGIRYGAPPDTFTTQMEMTFIVLALKHGYFVNSPDYEGFNSAFMVGRRSGQATLDSIRATLRSENITGIKPDAKVALWGYSAGAVVTAWASVLQPSYAPDLKPHLVGAAIGGFAPDFISMAKTLDGTFLSGLIPAALYGIANEYPVVHDIIDQHANPKLMSELRLGNTLCATPAALYYMAGNFMKGNEPFFLQGDAFLEHEVIKEILEDNSINRFQTIPEIPMFLYNGQLDQVIPIEDVRGIYSYWCDKGIESLEYAEDMLNGHFSGILFSAPAAWTWLENRFQGKEPVKGCSRTVRYFNLFYPNSSYATKSYMGGLVDLLMQNKIGPDFNNSSPRLTDILKNKLSGYIYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJQ5</accession>
    <name>G3AJQ5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sec39 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_59368</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373540.1">
        <property type="nucleotide sequence ID" value="XM_007373478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJQ5"/>
    <dbReference type="STRING" id="619300.G3AJQ5"/>
    <dbReference type="GeneID" id="18872880"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59368"/>
    <dbReference type="eggNOG" id="ENOG502RS0W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014756_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJQ5"/>
    <dbReference type="OrthoDB" id="342024at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013244">
        <property type="entry name" value="Sec39_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40787:SF3">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40787">
        <property type="entry name" value="SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08314">
        <property type="entry name" value="Sec39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Sec39" evidence="5">
        <location>
            <begin position="162"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW33956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="459" mass="52988" checksum="8FFD675BBE1695C9" modified="2011-11-16" version="1">MFSDLTVGLAKYKRWFTIIKSIVHSEFTSDDYREIIEWYIISCYYYAHKEDSLPSIEIINIYDVIYDTLSLFDQPVSVSDLSCADFIGCDIDIPQQLSTDSFKKVVSTCQNLFPITKLTIVKYLQYNQSVNNKDITKLISTLNHTNYHQLMPAITALKSQFNLDHGLINKLIIERLLASNLFDEVKEYQSELSSDEFFSLVIDKFWTSFNSATNLNDKIGHLHESKQCLDLSDSLQLNEENHDLIVRLKHLFKAMTNLKNFKIVVDKSKPFTPGQLLSFKDDLIGLITVILQQNPKSYLAFEKLFKIINDLALYFQAEGDFFNKVKIACIESSLIDDNFEYAYTHTKQLFTVNVDELWIIFYQVGKYISPNWTEPNINVLVKQREILSLTLKHISENNKIILDQWEKVNSQIEEYSEQVLNVSESKEASSDDIVENISTDKISNLLVSGLGWAIGAHHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJU1</accession>
    <name>G3AJU1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_59400</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373576.1">
        <property type="nucleotide sequence ID" value="XM_007373514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJU1"/>
    <dbReference type="GeneID" id="18872900"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59400"/>
    <dbReference type="eggNOG" id="ENOG502SGP3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_845110_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJU1"/>
    <dbReference type="OMA" id="YCSATSA"/>
    <dbReference type="OrthoDB" id="10266999at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07440">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RGS" evidence="2">
        <location>
            <begin position="91"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="329" mass="36489" checksum="2D45C1432068E87F" modified="2011-11-16" version="1">MMIASATEVNHESLFNYNLNSTSSSLAAISTQVTPISLHSPSPPTPHTPSSPSQQPQIISEPPQSSSPPTVIPTLDEILNTSINAQNSDFNLDKFVSYLSSVHGNENLEFVLDVNSYLSLRPPTISHWQQLYTKYIELEAPCEVNLPCHFRSQLTLNKMPDFTLLIKCRRYIHDDILINLYHEFVKHVKSAFECPFRCCTPPNDPTVSPRHSVHYVNNKAAPPLTHSQSVYCSATSAAISSSRLKHSNSVSGASMFRQPPPLHKHSQVGYDYYAIPNSDCSSTSEEDNSPRTTRNNSAGSVRGGSLGSLVGDYKSMIKKFKFRRSSTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK04</accession>
    <name>G3AK04_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_59477</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373639.1">
        <property type="nucleotide sequence ID" value="XM_007373577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK04"/>
    <dbReference type="GeneID" id="18872933"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59477"/>
    <dbReference type="HOGENOM" id="CLU_2211614_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK04"/>
    <dbReference type="OrthoDB" id="10416343at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013965">
        <property type="entry name" value="DASH_Dad3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08656">
        <property type="entry name" value="DASH_Dad3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="107" mass="12202" checksum="DAE6568E7B0AD89D" modified="2011-11-16" version="1">MSSRAVSVTDSDATNVSANFTDLEKEVLSEYKTLLVQLTTLNEDVKRLIQTHDSFDNQNVHGLMETWQQLEKQSGVVNKVFESALYDLIRNYDGAELPQQVSFEKME</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK11</accession>
    <name>G3AK11_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Very-long-chain 3-oxoacyl-CoA reductase</fullName>
            <ecNumber evidence="12">1.1.1.330</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">3-ketoacyl-CoA reductase</fullName>
            <shortName evidence="12">3-ketoreductase</shortName>
            <shortName evidence="12">KAR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Microsomal beta-keto-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_59486</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the microsomal membrane bound fatty acid elongation system, which produces the 26-carbon very long-chain fatty acids (VLCFA) from palmitate. Catalyzes the reduction of the 3-ketoacyl-CoA intermediate that is formed in each cycle of fatty acid elongation. VLCFAs serve as precursors for ceramide and sphingolipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a very-long-chain (3R)-3-hydroxyacyl-CoA + NADP(+) = a very-long-chain 3-oxoacyl-CoA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85440"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="EC" id="1.1.1.330"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Endoplasmic reticulum membrane</location>
            <topology evidence="12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.330" evidence="12"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373646.1">
        <property type="nucleotide sequence ID" value="XM_007373584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK11"/>
    <dbReference type="FunCoup" id="G3AK11">
        <property type="interactions" value="726"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK11"/>
    <dbReference type="GeneID" id="18872937"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59486"/>
    <dbReference type="eggNOG" id="KOG1014">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_38_0_1"/>
    <dbReference type="InParanoid" id="G3AK11"/>
    <dbReference type="OMA" id="LVAPGMM"/>
    <dbReference type="OrthoDB" id="5545019at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045703">
        <property type="term" value="F:ketoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141040">
        <property type="term" value="F:very-long-chain 3-oxoacyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030497">
        <property type="term" value="P:fatty acid elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05356">
        <property type="entry name" value="17beta-HSD1_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000317">
        <property type="entry name" value="Very-long-chain 3-oxoacyl-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03107">
        <property type="entry name" value="3_ketoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027533">
        <property type="entry name" value="3_ketoreductase_fungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43086:SF2">
        <property type="entry name" value="HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43086">
        <property type="entry name" value="VERY-LONG-CHAIN 3-OXOOACYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000126">
        <property type="entry name" value="11-beta-HSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 12" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0521">NADP</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="346" mass="37749" checksum="2E8C04A101396B4E" modified="2011-11-16" version="1">MSVEDLLSSLSGNRVVYYALYGALLFGVLKLTILGLSFGSMIFGLFILPPVNFKKYGAKQGKWAVVTGASDGIGKEYAFQLAKKGLNIVLVSRTQSKLELIATEIESKYKVQTKIVAFDASTDDDANYISLRNVVSELPITVLVNNVGQSHSIPVPFLETDEKELNDIITINNTATLKITQVVAPLIVSTIKKEKKKVRGLILTMGSFGGLLPTPYLATYSGSKAFLQAWSNALAGELKPQGVDVELVISYLVTSAMSKIRRTSATIPNPKQFVAATLRGVGRRNGAQERFATNTPYWSHAIMHFAIANTVGVYSKVANSLNFSMHQAIRKRALKKAERLAAEKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK17</accession>
    <name>G3AK17_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_59491</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373652.1">
        <property type="nucleotide sequence ID" value="XM_007373590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK17"/>
    <dbReference type="GeneID" id="18872941"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59491"/>
    <dbReference type="HOGENOM" id="CLU_2591313_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK17"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="80" mass="9136" checksum="48F9A2A6A8331014" modified="2011-11-16" version="1">MTSISKHQENFHTHCMQGVVAILDPLLMPTTIGNTISKNILDVHTQKPEKEIKKKKKMEILEGRRRLPNNHVSNELAPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK45</accession>
    <name>G3AK45_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">LrgB-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_70828</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374369.1">
        <property type="nucleotide sequence ID" value="XM_007374307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK45"/>
    <dbReference type="GeneID" id="18875349"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70828"/>
    <dbReference type="eggNOG" id="ENOG502QQ63">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024337_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK45"/>
    <dbReference type="OMA" id="LRWMNLF"/>
    <dbReference type="OrthoDB" id="2502820at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007300">
        <property type="entry name" value="CidB/LrgB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30249:SF0">
        <property type="entry name" value="PLASTIDAL GLYCOLATE_GLYCERATE TRANSLOCATOR 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30249">
        <property type="entry name" value="PUTATIVE SEROTONIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04172">
        <property type="entry name" value="LrgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="289"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="343"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="466"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="514"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="540"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW32854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="574" mass="64759" checksum="B5260496FC2D4A5B" modified="2011-11-16" version="1">MVPTVYMHHSLNQNNAGVVSHHVRRRNNQNTAIHSIWYSKTHLFNSYFVIPIGLLIILCTLYGLNQLLLIINIKFPCSVLGLLLNLLFLCLLSWLSHQNTFAKNGANWMLTNYLGLIKPSMNFTLKWINVFFIPSFIVLPLSSPISFIECLKIAGVFVVGMMVLICVDVYFLKLLKLVYAKMGRTDEKEHEEIELETLERDDITTIDLTSFAKDNDDDDVEVVTSRRTSENPFTIEEVSGVDIPEPTYQRDSDEDHQVTRQMSRISYVDQIQVEELSRSERVTLFTTKYIDWILYLILFIVSLPIYYSLHVYLPYHLSITILAYYVALLIPQKFPVTKKFAHPILISTFIILFVCFIGSLISHGKPRGFLLDLQYYKTGRNYLTLFTNQYVVWPGCGDFLSSLMDVSIVSLSLPMFTHRHDFIKNFWVLMPTLVISVVLTFFLYPITCHAIGIEPVRSIGFIGRSVTLALGTPLVTALGGSISLMAVCTILSGIIGVLINEPLFRLLRVDKGDYVTRGVTLGINSGAIATAHLLTVDPRAASISSLSFSVFGTIMVVLASVGAVRDLIRSWVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKB3</accession>
    <name>G3AKB3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor IIF subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">TFIIF medium subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">TFIIF-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_60912</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIF beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375086.1">
        <property type="nucleotide sequence ID" value="XM_007375024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKB3"/>
    <dbReference type="FunCoup" id="G3AKB3">
        <property type="interactions" value="519"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKB3"/>
    <dbReference type="GeneID" id="18873631"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60912"/>
    <dbReference type="eggNOG" id="KOG2905">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047858_0_1_1"/>
    <dbReference type="InParanoid" id="G3AKB3"/>
    <dbReference type="OMA" id="ANCPEHQ"/>
    <dbReference type="OrthoDB" id="26094at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001174">
        <property type="term" value="P:transcriptional start site selection at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07980">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000035">
        <property type="entry name" value="General transcription factor IIF subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003196">
        <property type="entry name" value="TFIIF_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040450">
        <property type="entry name" value="TFIIF_beta_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040504">
        <property type="entry name" value="TFIIF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011039">
        <property type="entry name" value="TFIIF_interaction"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02270">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17683">
        <property type="entry name" value="TFIIF_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50916">
        <property type="entry name" value="Rap30/74 interaction domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TFIIF beta subunit N-terminal" evidence="13">
        <location>
            <begin position="64"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIF beta subunit HTH" evidence="12">
        <location>
            <begin position="292"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="369"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="371"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00081473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW33571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="389" mass="44963" checksum="6CA2415429507646" modified="2011-11-16" version="1">MSTAKTVSPTKKLKKEQQPTVNDTPDEEALQSQADDPEDPSILSDPEDYLEENESLDMNLTGGNQKVWLVKLPRYLMEKWQNQEEIDGKQLGMVKIKKDTGSNGKLQVKLVLNNQENNMNDIPQEYDIKILNTQVRNTYAFSEENLKKFKQELTEVSQMPEQPQLPTDKDASKKKFVPRKKFKFFRVQKTGEDGQQVRKYIPFVKTIPKKTALMGKICHDCQIIPSKSDSKYGEIYMRRHNITQGPPRPKVTLLNEIPGVIQSNAGPSIKGSNTSVFLKSTIAKGKNEGRAIRMPKKDLLDLLFRLFEEYEYWSMKGLKERTRQPESYLKESLDSIANLIKKGPYTSKYNLKPEYRRLRDAERAARLGIDFTEEKEEEEEEDEEMEDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKT3</accession>
    <name>G3AKT3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_60313</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374500.1">
        <property type="nucleotide sequence ID" value="XM_007374438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKT3"/>
    <dbReference type="FunCoup" id="G3AKT3">
        <property type="interactions" value="250"/>
    </dbReference>
    <dbReference type="GeneID" id="18873337"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60313"/>
    <dbReference type="eggNOG" id="KOG1838">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032487_1_0_1"/>
    <dbReference type="InParanoid" id="G3AKT3"/>
    <dbReference type="OMA" id="MMTTSWG"/>
    <dbReference type="OrthoDB" id="5954035at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008126">
        <property type="term" value="F:acetylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051792">
        <property type="term" value="P:medium-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051793">
        <property type="term" value="P:medium-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000952">
        <property type="entry name" value="AB_hydrolase_4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012020">
        <property type="entry name" value="ABHD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF63">
        <property type="entry name" value="ALPHA_BETA HYDROLASE 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005211">
        <property type="entry name" value="Ab_hydro_YheT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01133">
        <property type="entry name" value="UPF0017"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="5">
        <location>
            <begin position="160"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4">
        <location>
            <position position="377"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4">
        <location>
            <position position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR005211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW32985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="464" mass="52706" checksum="ADE66F9A2B46F9FE" modified="2011-11-16" version="1">MLGLFTSQVENYIPNTPIKFTPRAEGEPTTTLPDLIKSKVPELAKGYSYFINPVLSSGHAQTAYTALNKFANSFHVHYKRRILTVENKTYTLPTGEKLKYDQWEGETTIALDYALQSHQNSDPEHVWYKPSSQTKELPPRTEYMNPELEPTIIDNSDDKPLLIVLHGLSGGSYEAYIRAVLDKIILDPFNFDAIVLNSRGCANHTITSPQLYNGLWTNDLRYVMNEHVSKLWPNKRVYLMGFSLGGAILANYLGQEGDAVSKQIKGACVLGTPWDFPDSAIHLRESIIGHNVYSPTMCSNLLKLLDSHGLLLSQELVKHFKDNPDQYNIKFLKQFDNYFTAKLFGLNSADEYYRLASPVQRLMKVRVPTVIISSRDDPITGYRTLPVSEVELNPYCTLVTTTVGGHLGWFTVTGDRWYTTPVCRLFTELDKYNVDHASVTEKDLPRDISTSWKYDRLVNEMLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKW0</accession>
    <name>G3AKW0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_49931</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374518.1">
        <property type="nucleotide sequence ID" value="XM_007374456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKW0"/>
    <dbReference type="GeneID" id="18871387"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49931"/>
    <dbReference type="eggNOG" id="ENOG502S976">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_479934_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKW0"/>
    <dbReference type="OMA" id="SHRYGGS"/>
    <dbReference type="OrthoDB" id="10671583at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="527"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="530"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="568" mass="50278" checksum="81115E0EA788B5F8" modified="2011-11-16" version="1">MAGIGGALGGIIDGIGGASGADGAGAALLATGGTTLGIGGAVGGTGGAGGAGGTEGALTFRLGGAAPKGLNFFFSSLGFSGTGVDASDEVEYPDFDPSTTNDSFLFELISARNGNPPAAGVGDFAFDDDGGGSDNVGGGGPGGGGGGAPPVGNTGTIGGGGGGGGGGGAPVVDNGGAGGGIGAAAGGGGGARGIAGGSMFGIGGGPEVGIGGGGGGPDAGIAGAFVLELILLFVGTESTTCLSSRFALISLRKGNGDASGGRAVAAAGVLDAGGGGGGGGGGADASAGGAAGGAAGGGGGGGGGMPIGIGGSGGAAIVGKGGGVLSSITELTESVLRWVDFLGSVGGIFGGLGETTGGGGGGSDPGIGGAAVGIGGAAVGTGGACIVGGGADRILRDFLWAGLEPVGTEGISGCLVGLEPVGTEGIGGWAAELVGIGILGADGGTIPDLLSTCFNFGIPPANISPNCGGPAEGIAGACEEDVDVALEGCGGAADTDTLSSMIGFDLSTVTAFFNFIPLRISPRRASLPGGIAGGGPASPKSGGGGGPGGGGGPGGGGGGGGGGGAAIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL24</accession>
    <name>G3AL24_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thiamine pyrophosphokinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_70985</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374582.1">
        <property type="nucleotide sequence ID" value="XM_007374520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL24"/>
    <dbReference type="FunCoup" id="G3AL24">
        <property type="interactions" value="57"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL24"/>
    <dbReference type="GeneID" id="18875353"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70985"/>
    <dbReference type="eggNOG" id="KOG4313">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048013_0_1_1"/>
    <dbReference type="InParanoid" id="G3AL24"/>
    <dbReference type="OMA" id="NLAKECW"/>
    <dbReference type="OrthoDB" id="10261522at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044715">
        <property type="term" value="F:8-oxo-dGDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03676">
        <property type="entry name" value="NUDIX_Tnr3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000019">
        <property type="entry name" value="Thiamin pyrophosphokinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031804">
        <property type="entry name" value="DUF4743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622:SF8">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15916">
        <property type="entry name" value="DUF4743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="136"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="316" mass="36360" checksum="D84671A37D173CFC" modified="2011-11-16" version="1">MTESNTTTTTFLDIINDVDSFKLNNTYYKFFSHDGVLLGYIAPSIAQYFPGLPITTKSFEINNVEQTIKIGSEFDTLEKRNEMFNKVALEWRDNITQLEHRLKKGWRNELYTVYNPTSIPYARIERAFSVLLGVVTYGTHINGYISEQNSSNGKLKMWIPRRAANKPTYPNMLDNMVGGGLGYPYGIWETVVKECYEEAGLSEDFVASHSKCTGVLSYLYGTADGRVQPEVEYVYDIEFENEHEVVPHPVDGEVSEFKLLDLDEILEKLRNKEFKPNCGLVIVDFLVRHGYITPESEPNYFEIVTRCHTKFEFPTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL58</accession>
    <name>G3AL58_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_137284</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007374554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL58"/>
    <dbReference type="FunCoup" id="G3AL58">
        <property type="interactions" value="836"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL58"/>
    <dbReference type="GeneID" id="18870034"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137284"/>
    <dbReference type="eggNOG" id="KOG2046">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055232_2_1_1"/>
    <dbReference type="InParanoid" id="G3AL58"/>
    <dbReference type="OMA" id="EVKQYLY"/>
    <dbReference type="OrthoDB" id="21595at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050606">
        <property type="entry name" value="Calponin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385:SF14">
        <property type="entry name" value="TRANSGELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="2">
        <location>
            <begin position="41"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="193" mass="22432" checksum="5B5F2E3CBA44DF2B" modified="2011-11-16" version="1">MSNYMSYDRYNQPTKLDQNANTNIDQDLANSRSTKYQERQLEVKQYLYDVITPHYPQLFTKKFSTDNTTSFIKTVDLMDMLKDGEVLCKLGSLLSDVPGNPTVKFKNSQMAFIQMENISFFLKLCELINLQHDEIFQTVDLYEKKDPYQVIVTLMSFSRLAHDIDSIKFPRVIGPKIVKVKPSVPNKPFKLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALA0</accession>
    <name>G3ALA0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF1014-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_60449</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
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            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC124 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007374596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALA0"/>
    <dbReference type="STRING" id="619300.G3ALA0"/>
    <dbReference type="GeneID" id="18873418"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60449"/>
    <dbReference type="eggNOG" id="KOG3223">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069723_1_0_1"/>
    <dbReference type="InParanoid" id="G3ALA0"/>
    <dbReference type="OMA" id="FEERMMP"/>
    <dbReference type="OrthoDB" id="76412at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010422">
        <property type="entry name" value="Ccdc124/Oxs1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054414">
        <property type="entry name" value="Ccdc124/Oxs1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054413">
        <property type="entry name" value="LSO1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21680">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21680:SF0">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06244">
        <property type="entry name" value="Ccdc124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22048">
        <property type="entry name" value="LSO1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LSO1/LSO2" evidence="5">
        <location>
            <begin position="9"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Coiled-coil" evidence="4">
        <location>
            <begin position="113"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="182"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="187"/>
            <end position="202"/>
        </location>
    </feature>
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        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF06244"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF22048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW33143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="24738" checksum="CF741F864338DB20" modified="2011-11-16" version="1">MAKKGGENSKKAAGQARKAEQANKKKQEVFAAAEAEEAALWDQGAKGGNKKQQEAQFKKEEAARKKAERDALLQAEEASMPSKPKGKDRGAAKTASKRTGKIDDFLDFNKDIPELSASGIESALEALALTGREGGVANKDIDRHPERRVKAAFAAYEDKRLPELRKENPGLRLQQIKHLLFKEFQKSPENPMNQDTNVSYNASRDDVDAKKKEVRSLRESKFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALF3</accession>
    <name>G3ALF3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60527</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374711.1">
        <property type="nucleotide sequence ID" value="XM_007374649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALF3"/>
    <dbReference type="STRING" id="619300.G3ALF3"/>
    <dbReference type="GeneID" id="18873449"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60527"/>
    <dbReference type="eggNOG" id="ENOG502SD15">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_445499_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALF3"/>
    <dbReference type="OMA" id="MMDDDAM"/>
    <dbReference type="OrthoDB" id="4095451at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
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            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="465"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="601" mass="70167" checksum="6F307E04142C4CFE" modified="2011-11-16" version="1">MTSFRPRYNNRYTRKNYFAGERPGHHRHGKHHRFQERPLKKYVYVEEPESDAEYADEESDEGETYEFDLNENEDADEDDYEEDYDSEESDDVVYYDGDDVVDLNDELENDAVDELEADGEVYDLNDELVRYYDDESDSELDSSDDVDSDVDPVDIYDIVDLNDQLQAEAIQQLLNGEVDSESDYDEEEDDEEEDDDDYDIEYSSNPKELRDQLILESVRQAFEEELVSDDEIALSEDEDSELGSEPEYFEVDSELEDDESDYDAGNPDNYDYEDDSSDSSDDEEGITIYKHRFDLPEGESEEDSTDDELVLYDNLSDDDEEEESDHELYDDDGLLQAENSDDEYDVIDLADELADSKDCELVQLDDHTYKLNLRLPSLVRDELKIEFLKNENQLLIKGKLNFNDIVESDLEVEEDDEDEEEDESEDDIEAEVVLQEVSDEEDDEEEEEEQDEEKEESESAAAPAEENEDEKEDDEEEEDDEDYEAESEVDDTMEYDWTGFEEDDEEDEEIQEDALSLIKDYKNQEICFEKHVEFDQLIKFEEIQARFVGDDELELIIPTEETPLEEANRYAITIAPYEEDKDSSTTDEIEAAVEEDVAMEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALP0</accession>
    <name>G3ALP0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60616</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374797.1">
        <property type="nucleotide sequence ID" value="XM_007374735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALP0"/>
    <dbReference type="FunCoup" id="G3ALP0">
        <property type="interactions" value="292"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALP0"/>
    <dbReference type="GeneID" id="18873493"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60616"/>
    <dbReference type="eggNOG" id="ENOG502RG16">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033352_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALP0"/>
    <dbReference type="OMA" id="QANDRIF"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013927">
        <property type="entry name" value="TF_Opi1_Ccg-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38406">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR OPI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38406:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR OPI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08618">
        <property type="entry name" value="Opi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="392" mass="43069" checksum="F146DA948854425D" modified="2011-11-16" version="1" fragment="single">MSSNNTTSATVTATSNSSRFNAMSSPDSLSDSASEHEALVSAAEALTRLTRSATPSSDADTIVSESLPSPTPQPPQLQHPLVSTVSMVAQHPLVKGAVKYYETSKRNCPPLNYAAGIFEKAAIPMVNKIEVNLNNRHQTKQALHSASSSADITPSISIDTFDNKQKKRRLTNDDKPPQVSDNKKRLQFCLHVLRLANDTINSKVNYLQEKVVETEMAVKEEREKIQQNENDEDVDTKTQKTKTEIVTTVKKIIRLISNFRPSTLSTDSLTPVSSNTSTSSSVQLQNYELKTAIRDIILSLPTSLQQPGTTTPGGTTQQSNDRIIVFAKESLEMISKLTNVFNDQLSKAESWVNGEEELDQNMEDVTVTSEVPSRASSADPEELTTATKRMKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALP2</accession>
    <name>G3ALP2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_66267</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374800.1">
        <property type="nucleotide sequence ID" value="XM_007374738.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALP2"/>
    <dbReference type="GeneID" id="18875094"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66267"/>
    <dbReference type="eggNOG" id="KOG1601">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061632_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALP2"/>
    <dbReference type="OrthoDB" id="273556at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051140">
        <property type="entry name" value="GATA_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658:SF18">
        <property type="entry name" value="PROTEIN GAT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="6">
        <location>
            <begin position="263"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="211"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="329" mass="37356" checksum="65F2F52079796AE2" modified="2011-11-16" version="1">MNGYSKDSGVTTRLPSFQQLMKGFQDSESPNNTTTIITQHLQPQPVQAQRLIQYSPGKLTPTATTFGNYTLNPEYPYHHHQHHYDNSYPQYPYPPGPSSVKSARASPMNRGRSLSFSYPQGTSPIQDILESGIICNEYQFISNLIQFLIEFEKKCHDFKLNYTNNPNDLSLISEKILTDFDDLDDALHNCNRVVKMLENIKILNEKTRVKRSNTSKRIDKRKLKKKSKTGSDGGSDLGLEINVAATANHDQVNIGGLNPELSIKPEITCQHCCSQETPEWRRGPEGSRTLCNACGLFYSKLIKKYGLQEADKVMYQRKQTGTVNDRRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALQ8</accession>
    <name>G3ALQ8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidylinositol N-acetylglucosaminyltransferase subunit GPI19</fullName>
            <ecNumber evidence="7">2.4.1.198</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_137815</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Part of the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + UDP-N-acetyl-alpha-D-glucosamine = a 6-(N-acetyl-alpha-D-glucosaminyl)-1-(1,2-diacyl-sn-glycero-3-phospho)-1D-myo-inositol + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57265"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="2.4.1.198"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the phosphatidylinositol N-acetylglucosaminyltransferase (GPI-GlcNAc transferase) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the GPI19 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.198" evidence="7"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374816.1">
        <property type="nucleotide sequence ID" value="XM_007374754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALQ8"/>
    <dbReference type="FunCoup" id="G3ALQ8">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALQ8"/>
    <dbReference type="GeneID" id="18870080"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137815"/>
    <dbReference type="eggNOG" id="KOG2257">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081616_1_1_1"/>
    <dbReference type="InParanoid" id="G3ALQ8"/>
    <dbReference type="OMA" id="FTDEHAV"/>
    <dbReference type="OrthoDB" id="690928at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017176">
        <property type="term" value="F:phosphatidylinositol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052263">
        <property type="entry name" value="GPI_Anchor_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013717">
        <property type="entry name" value="PIG-P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016542">
        <property type="entry name" value="PIG-P_GPI19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08510">
        <property type="entry name" value="PIG-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008765">
        <property type="entry name" value="PIG-P_GPI19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 7" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="6 7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="PIG-P" evidence="10">
        <location>
            <begin position="32"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR008765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="167" mass="19232" checksum="D755A2B416262A34" modified="2011-11-16" version="1">MLSRSNSPRPNQEDTLARESDVTVSTNVTQNAEYKGFAVYVIATISLLVWISWTLLPESTLHKLSIYYYPDRYWALAIPSYSLVLMAFIYWVLALYNTEVLTIPLNDIRTIVDEHSHFPGQSDEKLTRKQLMDLACDYVHDAPSGVWDLPITLVNEVLYNNDDDREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM10</accession>
    <name>G3AM10_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Vacuolar sorting protein VPS33/slp1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_136646</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374878.1">
        <property type="nucleotide sequence ID" value="XM_007374816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM10"/>
    <dbReference type="FunCoup" id="G3AM10">
        <property type="interactions" value="734"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM10"/>
    <dbReference type="GeneID" id="18869989"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136646"/>
    <dbReference type="eggNOG" id="KOG1302">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_344541_0_0_1"/>
    <dbReference type="InParanoid" id="G3AM10"/>
    <dbReference type="OMA" id="AYCGVVP"/>
    <dbReference type="OrthoDB" id="10262287at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043155">
        <property type="entry name" value="VPS33_dom3b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="684" mass="78817" checksum="FB0B54DB00E6AAB2" modified="2011-11-16" version="1">MTQESLNLNQFNESILNNLLTQLSKIYSSNNLLILDPILSPLLNNLTPFNRLKEAGKFQNIVWLNNEITSIQHQVFSKYTSLIIIIPGTSENLELLHKFIPNILNHVHGLKMNLIVKDLSRSLLYQINNLFDGILKFDSILQDDTKFDKPITITTGIKVFNWLTGPIHTDGILITEINEYSGIDNYFKSPLFQVNQLTKSLIQLLFHNKSIGKLLKLKNIYGKGNHSDLLIKELTNVKIPEYLSENLSKLEVEFYESQLHSNTNLIVLERNLDFMPLLFNQLNYHGIIDDLFDIRFDTIENLEEDVSKTLVGDELYQQDLKDLNFSSIGARLNKLAKFIQQQYKSSNATDDNLKDMKQLVSNLGNLTLQQDLIKKHTIIGESVLDKMNNEYQEFLEFQNDLFEMDYKLQLSKLKYFFNCNFPINFIWSGLLLVGYINDGISSKDLDWISQELQDNYGLGAIISLEQLIKYKLIRINHDSSSDFLSNLGLTQKKQTPVHSHSEEDYQVGITGGQDIFKSNYTLINKFWNLHPLEEEDAPETNQDSTNLLEMYSNPSFTLPGNTVPLTYRIVESLYFRDFLKYKPINNIKKRPNWDNLGLGTMFSGKTIDVNIDDHSDDKNNPQKGNLVANPEYIIIAIIGGITRSEITCFKYLQERLTRSDKNKKIIVMSNGIVNNSKLFKFINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM13</accession>
    <name>G3AM13_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Isocitrate dehydrogenase [NADP]</fullName>
            <ecNumber evidence="11">1.1.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_60714</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19629"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="11 14">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="11 14">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="11"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374881.1">
        <property type="nucleotide sequence ID" value="XM_007374819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM13"/>
    <dbReference type="FunCoup" id="G3AM13">
        <property type="interactions" value="821"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM13"/>
    <dbReference type="GeneID" id="18873534"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60714"/>
    <dbReference type="eggNOG" id="KOG1526">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023296_1_1_1"/>
    <dbReference type="InParanoid" id="G3AM13"/>
    <dbReference type="OMA" id="HGTVQRH"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006739">
        <property type="term" value="P:NADP+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000002">
        <property type="entry name" value="Isocitrate dehydrogenase [NADP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004790">
        <property type="entry name" value="Isocitrate_DH_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00127">
        <property type="entry name" value="nadp_idh_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006156">
        <property type="entry name" value="PRK08299.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822:SF21">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NADP], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822">
        <property type="entry name" value="NADP-SPECIFIC ISOCITRATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000108">
        <property type="entry name" value="IDH_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="6 11 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 11 14" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 11 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11 15" id="KW-0521">NADP</keyword>
    <keyword evidence="8 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="16">
        <location>
            <begin position="23"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="89"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="108"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="323"/>
            <end position="328"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="12">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="12">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW33366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="422" mass="46962" checksum="227F00FE14EA0B45" modified="2011-11-16" version="1">MIRAARSFSSTTKTLAKIKVTNPIVELDGDEMTRIIWHRIREQLVTPYLDVNLKYYDLGIQSRDATNDQITIDAANAIKEYGVGVKCATITPDEARVEEFGLKKMWLSPNGTIRNILGGTVFRESIIIPRVPRLIPGWKEPIVIGRHAHGDQYKATDLVITEPGKLELTFTPANGGAPETRTVYDYKGTGVGLAMYNTDESIRGFAHSSFKMALTKGLPLYMSTKNTILKKYDGRFKDIFQEIYESDYAKEFEAKGLWYEHRLIDDMVAQMIKSKGGFVMALKNYDGDVQSDIVAQGFGSLGLMTSVLMTPDGKAFESEAAHGTVTRHYRQHQQGKETSTNSIASIFAWSRGIAQRGRLDNTPDVVDFANTLEKATIDTVQEDGIMTKDLALATGRTDRASYVTTTEFLDAVADKLKTKVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM45</accession>
    <name>G3AM45_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_137101</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374265.1">
        <property type="nucleotide sequence ID" value="XM_007374203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM45"/>
    <dbReference type="FunCoup" id="G3AM45">
        <property type="interactions" value="120"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM45"/>
    <dbReference type="GeneID" id="18870015"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137101"/>
    <dbReference type="eggNOG" id="ENOG502QTKT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027285_1_0_1"/>
    <dbReference type="InParanoid" id="G3AM45"/>
    <dbReference type="OMA" id="KRTMITE"/>
    <dbReference type="OrthoDB" id="941679at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000111">
        <property type="entry name" value="Soluble cell wall protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050732">
        <property type="entry name" value="Beta-glucan_modifiers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631:SF14">
        <property type="entry name" value="FAMILY 17 GLUCOSIDASE SCW10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00587">
        <property type="entry name" value="GLYCOSYL_HYDROL_F17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442469" evidence="13">
        <location>
            <begin position="18"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="74"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="81"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW32750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="374" mass="38633" checksum="375D0E0DBABB9620" modified="2011-11-16" version="1">MLLNTLVKAALLAQALAAPVVQQHQHHQHQDKRAVHVVTKTNVVVVTVGAGDATVTLAPVDLPQPTADVKVSAVTDAPQNAPEAATSTAPAPPAESTSSSAAAPASTGSSQPDSGSVGAGGAKGITYTPYSDDGGCKSGSQIASEIASLSGFDVIRLYGVDCDQVAQVLQAKSSSQKIFAGIFDVSNIGAGVQAIADAVNAHGSWSDIYTVSVGNELVNSGQANPSQIGQYVSTARSALTSAGYSGPVVSVDTFIAVINNPELCQYSDYIAVNAHCFFDGHYTAEDAGPWVLQQIQRVWTACNGAKNVFITETGWPSQGDANGVAVPSKPNQEAAINSIKSTCGDSSILFTAFNDLWKTDGPFNAEKYWGIFSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMC5</accession>
    <name>G3AMC5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_151502</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRN7/TAF1B family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374938.1">
        <property type="nucleotide sequence ID" value="XM_007374876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMC5"/>
    <dbReference type="FunCoup" id="G3AMC5">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMC5"/>
    <dbReference type="GeneID" id="18870896"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_151502"/>
    <dbReference type="eggNOG" id="ENOG502RYCI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016553_3_0_1"/>
    <dbReference type="InParanoid" id="G3AMC5"/>
    <dbReference type="OMA" id="TICQFGH"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070860">
        <property type="term" value="C:RNA polymerase I core factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001164">
        <property type="term" value="F:RNA polymerase I core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048538">
        <property type="entry name" value="Rrn7_cyclin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048540">
        <property type="entry name" value="Rrn7_cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033599">
        <property type="entry name" value="TAF1B/Rrn7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021752">
        <property type="entry name" value="TF_Rrn7_Zf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31576">
        <property type="entry name" value="TATA BOX-BINDING PROTEIN-ASSOCIATED FACTOR RNA POLYMERASE I SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31576:SF2">
        <property type="entry name" value="TATA BOX-BINDING PROTEIN-ASSOCIATED FACTOR RNA POLYMERASE I SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20645">
        <property type="entry name" value="Rrn7_cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20644">
        <property type="entry name" value="Rrn7_cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11781">
        <property type="entry name" value="Zn_ribbon_RRN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRN7-type" evidence="10">
        <location>
            <begin position="6"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Rrn7/TAF1B N-terminal cyclin" evidence="11">
        <location>
            <begin position="104"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Rrn7/TAF1B C-terminal cyclin" evidence="12">
        <location>
            <begin position="221"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF11781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW33423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="479" mass="55455" checksum="B43C709623C5583F" modified="2011-11-16" version="1">MAKSAWFRGPVCGTDNCRSRLYRSTDGLTICQFGHVLEGAVEFNDDQDQAIVSTRRINAAVTMDSRGSYASSQPLGMSQSQISTQRESERLFGNEAVVLYYRCLQLLLKHELNVFVGLFCSESTMKDLTCLVKKNWIDVLEKDVHDEETELKDMHIDTLDIIVIIYVSALQLRAYSIYTTDILENIFTNQIPYARTLHLIPKEMLDKLPTVFHNRLQPYALPTRNQIYKKIKNTLNRIMSKSEFVMPISFYFPLIFRTYSETLLLPNAPDLFLIMYKLLQKLDITQLEIKYKSKTQTYMQSIPEVYLSMLMIFITKLSFYNKTNNFSLRDWLTQLNNHPAGSDTNFVQDSNVLDWSDSKVENYCDWIYDCVIPKKHKLNEANQQKLTTLEKRLFSIFKLDNDDLSNSQQNGSKPSEANADLHDIVTKLASTASENLSNEDFTQLDETLFSIFSHFLGLDKDDLIRCYDTITNDLRKKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMI9</accession>
    <name>G3AMI9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_50314</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374948.1">
        <property type="nucleotide sequence ID" value="XM_007374886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMI9"/>
    <dbReference type="FunCoup" id="G3AMI9">
        <property type="interactions" value="220"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMI9"/>
    <dbReference type="GeneID" id="18871426"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50314"/>
    <dbReference type="eggNOG" id="KOG0104">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_5_0_1"/>
    <dbReference type="InParanoid" id="G3AMI9"/>
    <dbReference type="OMA" id="DYGQQNI"/>
    <dbReference type="OrthoDB" id="10262720at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034663">
        <property type="term" value="C:endoplasmic reticulum chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10230">
        <property type="entry name" value="ASKHA_NBD_HSP70_HYOU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF3">
        <property type="entry name" value="HYPOXIA UP-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012790974" evidence="7">
        <location>
            <begin position="16"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="854"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="854"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="867"/>
            <end position="903"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="912" mass="102621" checksum="FDCD99C1A86B5512" modified="2011-11-16" version="1">MKFLLLAYLVASISAAILGLDYGQQFSKAAILAPNIYFEIVLTDEGKRKDLSGICLRNATQGIERIYGSQMGSLVTRFPQSCALDLKQLLGKNISDPTAQQYIKTHPGVKLIGDSKRNGSIKFDLGLGEKNQFSVEELLAMTLQEFKKRAIKLIESTPGQNSPVIDGIVVAIPPFASQATRQAYLDSLYIANIPNVLGLVDEGTSVALDYVTKKLANDDTPKEKKYHLIYDVGAGYTTATVFSLSRNDTALLVEMESFGYDPLFGGRLLTESVYDIIVDKLLSHFNIRSSDLTPKIQARLFEVAEKAKNVLSANMEYQTMIESIYDDKDFKVHVTREEFEEANEVRAEEISKPIEQALAATNLTVDNIQTVVLNGGSSRVPFIQKRIAEIIGEAKISKSVNTDESSVLGTTFRGLDLKTEGKGIKGYTLVEKNYHNYEAKVDDGELISVFPRGSKLGRRKVKLGEVGNSSVVELFEDGNLIKTYKLKDLGSYKLKCTEDQTKELVARFNIDNNKIVTLDTASLVCRDPASQHKDSFFDKLLHKKKSTTEEDDDEIEIKTGNVTNTTATKPTKARKRSPQPIVVPEPEIAGVSSISRVSMFESLKRIDSLNKQDKARAEFENAKNELEGQCYNLRSYIEDKEEVLLKEITDSKISEYNDFVGEVIEWLDFESDDSTIDDILAKAKEIAGKHSEIVDYVRLVTTDLSKSSLEKLYEDGSKFVMSIQSNMMEFGQQINELRQKYQEKAFDFDKENDRLKNKFFKSSDRIMNFDKDMATYREVITEFGNVLNSDIAKYSKNVLFELYDKLSKGVEQMKEDIKYLEDMNKERLEYLEDRFTKLVEREKQRQYRSLLKEYSKSAEEEAKTKTESGSSASEESSSTTESSQESGESSTSSQESSETTSTSQPDVDHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMW3</accession>
    <name>G3AMW3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_61454</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375653.1">
        <property type="nucleotide sequence ID" value="XM_007375591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMW3"/>
    <dbReference type="STRING" id="619300.G3AMW3"/>
    <dbReference type="GeneID" id="18873903"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61454"/>
    <dbReference type="eggNOG" id="ENOG502S42T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086434_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMW3"/>
    <dbReference type="OMA" id="DNACHTE"/>
    <dbReference type="OrthoDB" id="3998251at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025928">
        <property type="entry name" value="Flocculin_t3_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13928">
        <property type="entry name" value="Flocculin_t3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442267" evidence="6">
        <location>
            <begin position="18"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="112"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="203" mass="20470" checksum="DF9AFCDBFFBFB56B" modified="2011-11-16" version="1">MQYKLLATAALASTVAAASSGVPANSTETVTDLSTTLVTITHCEENKCSATVSPALKSVATVTVQGVETVYTTYCPLTSEEAAKSTLSTITTTVEGVETVYTTYCPLTEEETAAPAETPAPAPEKESSAPAPAPEAPITSVVEAVTTPVATVSEAGESTVYIQSTLYTSLANKTAPVSEFEGAAGKQAVAGFAAVAGLVAALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANE5</accession>
    <name>G3ANE5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61037</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375210.1">
        <property type="nucleotide sequence ID" value="XM_007375148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANE5"/>
    <dbReference type="GeneID" id="18873693"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61037"/>
    <dbReference type="HOGENOM" id="CLU_1918396_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANE5"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442455" evidence="2">
        <location>
            <begin position="17"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="132" mass="14966" checksum="C87855B20CF1C517" modified="2011-11-16" version="1">MLCGIILISLAYFLDPDSTEVSTLNTEPAADPLVQDVDSHKLTNVVVNIGLVLHEFWKNYLIYATLEFYWYSVIVFNVAYYLFSCGTISAAMLDAQMPGGELVSRRTCKITGTMLLISNWVRFTLRSYLDHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP66</accession>
    <name>G3AP66_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zinc finger PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_72003</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375913.1">
        <property type="nucleotide sequence ID" value="XM_007375851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP66"/>
    <dbReference type="FunCoup" id="G3AP66">
        <property type="interactions" value="157"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP66"/>
    <dbReference type="GeneID" id="18875406"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72003"/>
    <dbReference type="eggNOG" id="KOG1844">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005191_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP66"/>
    <dbReference type="OMA" id="RLPWEWD"/>
    <dbReference type="OrthoDB" id="20872at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034967">
        <property type="term" value="C:Set3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
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    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UPSET, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20826">
        <property type="entry name" value="PHD_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger PHD-type" evidence="6">
        <location>
            <begin position="446"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="25"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="368"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="514"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1020"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="42"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="201"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="238"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="385"/>
            <end position="399"/>
        </location>
    </feature>
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        <location>
            <begin position="421"/>
            <end position="435"/>
        </location>
    </feature>
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        <location>
            <begin position="514"/>
            <end position="544"/>
        </location>
    </feature>
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            <begin position="551"/>
            <end position="569"/>
        </location>
    </feature>
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        <location>
            <begin position="571"/>
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        </location>
    </feature>
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            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1148" mass="129118" checksum="8CF873DF95B08769" modified="2011-11-16" version="1">MTNKEEEQLLQDASTLLMFANVAAKQQNQHTIRQVSPPPQASYPQQQQQLQQQQQQQQPQPQPQPVQSQQKQPQQSPMATFSEPVPASAHYPQQPQQQPVRPHIRESPPSQHNQFPLPQYTFPVMYAGPRPPYSLPQPQQSQIAQPPIQILQPQKPPTQDAAVRKHSHPEQTIHDVTPRQPHRSSISILMNTPEPNTEVKQQVPPQTQPQVQTNAPGLSQTEQKQPPQMQPFTHIKSKSQSPMSQVNQNTPAPTSAASRKSVSPPSKLPKQGNFKPTHERSRSTPESNATIKFRHLQVSPTGNKFARGIDLETGERNTTNAVIAAAALTAAADVPLPLKHGDGKRNSSSASSSPILATSKATVVKLEALPSEKKEEDQLTEPEPETEKTDDEKTEDETAAKLSKGSSSSPPAQQKIKDENTVSPATKFQAPPLSTYQVDPDSGLIGCICGIEDDDGFTIQCDVCYRWQHCLCMGFQSADEVPEDEYKCYYCDESTWGKFDPELCRQQTLARLQNERMTSSSQDRSEYSNNNVAPQQTEQNNAPPSNKRRQSGPEKQAEKKKKLDEKKDTAPGTTTAQQAKSPSQPLEQSHEDETLPNKDNELLEDGITAESYQSVYYKLKQNDYKKQSIKEFIEHVGHEFYQGYLSLPKADQQKKVVNNVEIMTFNQFKALKMSKVHLPNHRKYLQEHHKFSKKKNFNKTTIQVKPYSDNQKQKFNGISRLSLFISGSNGGSSPVNGASEAIVIPENTPVIEYLGEIDLFKNYRNDSTNQYTSWGTTKPKVLKTAIPSKDGEVSLVLDSRFVGNESRFIRKSCPSSANCRIQPVYIPEKNTFKFMVLTSKPITLKAESHDEELRLPWEWDVEHPILKLYENNHSEKFENLTNVEKSALITYIDNILNFVECGCSSSNNLSTCAIFKIKKATSYLLRSTRKANSISNVNLMKSKDELILPRPSKEYISWEERLIKRDLELQSKFLGASAELLDQTLVNNIEEQESAVDKPPMLFKIPFKQQLITRIKVSPEELAPEADPDAMKEDQEEHEESNEDIPFPVVPDLVISIDRTIEENLKPIVKEVETKALQPLVDEPDLLDTKSEVEIIQKVVAATVNPVAEPVPEVVVSKKIAELKETVAPTAPKVVKKLSFADYKKKMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP68</accession>
    <name>G3AP68_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal lysine N-methyltransferase 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_51175</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007375853.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="G3AP68">
        <property type="interactions" value="146"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP68"/>
    <dbReference type="GeneID" id="18871529"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51175"/>
    <dbReference type="eggNOG" id="KOG1337">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033565_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP68"/>
    <dbReference type="OMA" id="EVDAYHE"/>
    <dbReference type="OrthoDB" id="441812at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF34">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE SETD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="498"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="645" mass="74642" checksum="9AF55DE1A98C4C17" modified="2011-11-16" version="1">MSSTLATKVDSLVEWLDTNSHWREDLIIKESQYGGLGVFYSEQPEEEFDPLLLRIPKTNLLAPKNSFIYNLLEEYEPVNPELVISEGVYALVVSFIYEHSIKHNSPWFEYIESMDIKSSEIPICLWDEADKANLANTEVDLLNMLDPKELIDFYIEVLHFADVNKTFVVIPQVLDIPTEHFSHVDILEKHKDKLVLFGKFLQTAISRAFEVDNYYGLALVPAADIFNHIEPELVNGKVKGREDIHFVCDESVCGICGESACDHEDSDDEDIENNDMEEDMEYENGDVQDSSAGEGELSSEESAEDDSDNGEEHLAHSEEEEEEEEEEEEEEEEEEEEEEEEEEEEEPIKELTMEHIYELEEELFNEVDTDLDPEEVSTVSDEEIQVDNGRMADLAQELSDASKCCDVVLVRRPEASTELFNSYGNELSNAYLLQKYGFISKENVNDSCILSVQFFKQIKSLKEKLNTEKRREIEEKIEWLEKLGYDLINQILAGDHDREHEHEHGHGHGHGHDHDHDDECEDDCQDDCCQPQEFPESWPLSIRINFNGECTPQTYAILKLLELKYVHFKYKLLSTTKERKLIPRIKKLLLTHSPQELATYNKRVSAWCQARIDRYPALTLSKHEQLIDSMKSQEIRILQKFIHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP76</accession>
    <name>G3AP76_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">10 kDa heat shock protein, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_61712</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GroES chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375923.1">
        <property type="nucleotide sequence ID" value="XM_007375861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP76"/>
    <dbReference type="FunCoup" id="G3AP76">
        <property type="interactions" value="802"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP76"/>
    <dbReference type="GeneID" id="18874017"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61712"/>
    <dbReference type="eggNOG" id="KOG1641">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132825_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP76"/>
    <dbReference type="OMA" id="EDFLIMR"/>
    <dbReference type="OrthoDB" id="184876at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045041">
        <property type="term" value="P:protein import into mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.33.40:FF:000002">
        <property type="entry name" value="10 kDa chaperonin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="entry name" value="GroES chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00580">
        <property type="entry name" value="CH10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772:SF0">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00056825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="11272" checksum="4B954EA451B2ACF0" modified="2011-11-16" version="1">MSFLKNASSLKPLFDRVLVQRLKPATKTATGIYIPEKNQEKLNQATVIAAGPGITNTTTGQVIPTSVKAGDKVLLPNFGGNPVKIGEEEYLLYTDKEILAKIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3APE6</accession>
    <name>G3APE6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Coenzyme Q-binding protein COQ10 START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_61207</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for the function of coenzyme Q in the respiratory chain. May serve as a chaperone or may be involved in the transport of Q6 from its site of synthesis to the catalytic sites of the respiratory complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with coenzyme Q.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the COQ10 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375399.1">
        <property type="nucleotide sequence ID" value="XM_007375337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APE6"/>
    <dbReference type="FunCoup" id="G3APE6">
        <property type="interactions" value="144"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APE6"/>
    <dbReference type="GeneID" id="18873777"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61207"/>
    <dbReference type="eggNOG" id="KOG3177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079653_1_2_1"/>
    <dbReference type="InParanoid" id="G3APE6"/>
    <dbReference type="OMA" id="IDGPFKY"/>
    <dbReference type="OrthoDB" id="292693at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140104">
        <property type="term" value="F:molecular carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07813">
        <property type="entry name" value="COQ10p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044996">
        <property type="entry name" value="COQ10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005031">
        <property type="entry name" value="COQ10_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901:SF10">
        <property type="entry name" value="COENZYME Q-BINDING PROTEIN COQ10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901">
        <property type="entry name" value="SPERM PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03364">
        <property type="entry name" value="Polyketide_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coenzyme Q-binding protein COQ10 START" evidence="4">
        <location>
            <begin position="42"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="196" mass="23050" checksum="95C8A58B216BFD55" modified="2011-11-16" version="1">MLVRIRPVLPIRPLTSAIFPRRTFFGSSKPQSYEISRTLNGTPRQLYDIVSDVAKYHEFVPFVEESFITERCSRNTPSRAGLQVGWKDINERFECKLNCVPGELVQATSLELDLFENLETKWKFNEVDGNKNKCKIDFQLVYKFRNPLYDRLSFMFAPQVSDIMIQAFQKRLFHIKREEAKLKYEDKRADVGLEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3APF9</accession>
    <name>G3APF9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_50725</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375412.1">
        <property type="nucleotide sequence ID" value="XM_007375350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APF9"/>
    <dbReference type="STRING" id="619300.G3APF9"/>
    <dbReference type="GeneID" id="18871478"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50725"/>
    <dbReference type="eggNOG" id="ENOG502T610">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_900143_0_0_1"/>
    <dbReference type="InParanoid" id="G3APF9"/>
    <dbReference type="OMA" id="WRANSID"/>
    <dbReference type="OrthoDB" id="4086732at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="368" mass="41814" checksum="3C234FD5C4FCED58" modified="2011-11-16" version="1">MDDDMRYDRPEKGKEDFSYSNISDDEEEFDDTEIETPVRLVSTTRLDLGRFDLPAPQPQRSGTTNYTKFRTLRRSPLRNPPIVASESAADKTSTKTDKPAVNGISKITHEKELPHSPPSNLKFNNSALSPPAAKKLGLILEPSEEQRPRKQIRKMLERASSDITEEPSFHKPDPIQTDGSDNRGTQSPGVLADVSREIIIEKVNDTINSLMQSKNKKPHIKVNADVKIPPIETKNDEISSEEEPELQVHHWSDMPEMLGVSTPLVERPSRKRNRSSIEDPVTTLDTGYKHTTTHSNWWPRSKWDKLLKIVLSKHIDREEAINSPLLMSELGCKSRVELENRYDFLVHYKLKAYNGHGNNGRASGRSQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3APJ7</accession>
    <name>G3APJ7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61259</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375444.1">
        <property type="nucleotide sequence ID" value="XM_007375382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APJ7"/>
    <dbReference type="GeneID" id="18873801"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61259"/>
    <dbReference type="HOGENOM" id="CLU_2470502_0_0_1"/>
    <dbReference type="InParanoid" id="G3APJ7"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="88" mass="10325" checksum="721A1CFBF5F38B18" modified="2011-11-16" version="1">MIPSPTSDKLLLSEHQFYAKSSTQPPKSSICHMFNDLRSFDLSDLFEVENIFRLLIVLVISVTLYKFIRSYFRRRTISKERSKPITPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3APY2</accession>
    <name>G3APY2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cAMP-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_61383</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.11" evidence="2"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375579.1">
        <property type="nucleotide sequence ID" value="XM_007375517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APY2"/>
    <dbReference type="STRING" id="619300.G3APY2"/>
    <dbReference type="GeneID" id="18873866"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61383"/>
    <dbReference type="eggNOG" id="KOG0616">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_5_1"/>
    <dbReference type="InParanoid" id="G3APY2"/>
    <dbReference type="OMA" id="KHTVVKL"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010607">
        <property type="term" value="P:negative regulation of cytoplasmic mRNA processing body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010737">
        <property type="term" value="P:protein kinase A signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05580">
        <property type="entry name" value="STKc_PKA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF153">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="65"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="320"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW32303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="377" mass="43584" checksum="869848450D322455" modified="2011-11-16" version="1">MTSMEGVVQGTDMDNHAATKQHQQQIYNTGKPNLKENDSQVLTKKISRHSDKDTTTKGKYTLNDFQILRTLGTGSFGRVHLTRSVHNGRFYAMKVLKKERVVNMKQVEHTNDERKMLKLSQHPFIIRMWGTFQDCHNLFMIMDYIEGGELFSLLRKSQRFPTPVAKFYAAEVFLAIEYLHNLDIIYRDLKPENILLDKNGHIKLTDFGFAKEVTDVTYTLCGTPDYIAPEVVATKPYNKSVDWWSFGILIFEMLTGYTPFYDPTPMKTYENILNGTITYPDYLPPDILDLLQKLIVKDLTQRLGNLQGGSDDVKNHPWFKEVIWERLLSRDIETPYEPPITSGIGDTSQFDRYPEDKDLDYGISDVADPYGELFQDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3APY7</accession>
    <name>G3APY7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_55793</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375585.1">
        <property type="nucleotide sequence ID" value="XM_007375523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APY7"/>
    <dbReference type="GeneID" id="18871972"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55793"/>
    <dbReference type="eggNOG" id="ENOG502S6RY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056040_1_0_1"/>
    <dbReference type="InParanoid" id="G3APY7"/>
    <dbReference type="OMA" id="GYEHWQW"/>
    <dbReference type="OrthoDB" id="2560628at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42109:SF2">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42109">
        <property type="entry name" value="UNPLACED GENOMIC SCAFFOLD UM_SCAF_CONTIG_1.265, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="249"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="304" mass="34353" checksum="184B50F2717C8806" modified="2011-11-16" version="1">MTFVDRNSGIAASIFLVLYVIYLSLTIKIVSEKGFRSIYTSLLVYGVFRVCGQMCGVVFSALGYEHWQWLIAYLVFSAEGYFVLVLCSFHLIANAQLYQFGQSWFRPSKEQKKQNLARATTIREKLRARFPLASVFHILLIPANALVISGGSTLAGQDPSNPDPNKIITAKALRTTGQVMFLSLTLIAILLAIYVYYKEGVRHCNIYSIFIVAPFITIRGIFGVISIFITKMNYYDMSNYTANGLSAQFVTYEYVLATTMEFITACVYVGNYFIDRQRQTIPVSNEFDDVFEDKLPSKVDQDSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ50</accession>
    <name>G3AQ50_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Short-chain alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RDH1</name>
        <name evidence="4" type="ORF">SPAPADRAFT_67464</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007376113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ50"/>
    <dbReference type="GeneID" id="18875163"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67464"/>
    <dbReference type="eggNOG" id="KOG1208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_6_1"/>
    <dbReference type="InParanoid" id="G3AQ50"/>
    <dbReference type="OMA" id="NEEGIWP"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
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        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF236">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="333" mass="37330" checksum="2BABD711EBFD4A0D" modified="2011-11-16" version="1">MSGPVVPPRGWEQWKQIIHGFFPGKPKFTEGQYPSFEGKVVIVTGGNTGVGYETVKSLAGSTKAKLYIFSRSEEKTLEAIKQIQLEVAKEYNISNREIHFIKVDLSDLETIKPAVQKFLSQESRLDLIIHNAGVMTPPVGSKSKQGYELQLGTNTIGPHLLQRLLDPIFIETSKKNAPGVSRIVWVASSGHQFAPLGGFHWDDVNFEKMKVTNNDQMMIYGQSKAGNVIQARTWTRKHNTGSQIISSSICPGFLDTDLQRHISSFEKVMYKFILHPRRMGAYTELFAAFSPDVKDGSHSISFGVPGHARKDLLDPEVCDKVWNYLNKVTDPYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ81</accession>
    <name>G3AQ81_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Small ribosomal subunit protein uS2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">RPS0</name>
        <name evidence="12" type="ORF">SPAPADRAFT_61993</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes).</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with RPS21.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376206.1">
        <property type="nucleotide sequence ID" value="XM_007376144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ81"/>
    <dbReference type="FunCoup" id="G3AQ81">
        <property type="interactions" value="1359"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ81"/>
    <dbReference type="GeneID" id="18874139"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61993"/>
    <dbReference type="eggNOG" id="KOG0830">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058171_0_1_1"/>
    <dbReference type="InParanoid" id="G3AQ81"/>
    <dbReference type="OMA" id="VKNFFEP"/>
    <dbReference type="OrthoDB" id="414863at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000010">
        <property type="entry name" value="40S ribosomal protein S0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03015">
        <property type="entry name" value="Ribosomal_S2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032281">
        <property type="entry name" value="Ribosomal_uS2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027498">
        <property type="entry name" value="Ribosomal_uS2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005707">
        <property type="entry name" value="Ribosomal_uS2_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01012">
        <property type="entry name" value="uS2_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11489">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16122">
        <property type="entry name" value="40S_SA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS2 C-terminal" evidence="11">
        <location>
            <begin position="202"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="212"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="261" mass="28748" checksum="76F42DFB115EA5AD" modified="2011-11-16" version="1">MSLPASFDLTPEDAKLLLAANVHLGSKNVQVHNKPYVYKTRPDGVNIINIGKTWEKIVLAARIIAAVPNPSDVAVCSSRTFGQRAVLKFAAHTGATAIAGRFTPGNFTNYITRSFKEPRLVIVTDPRTDSQAIKESSYVNMPVIALTDMDSPSEYVDVAIPCNNKGKHSIGLIWWLLAREVLRLRGIIADRTTEWSVMPDLYFYRDPEEIEQNAAEEAKADEAEEVQAPEAETEWTGETEEGDWADSGATPAQEDAAASNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQC1</accession>
    <name>G3AQC1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_67527</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376246.1">
        <property type="nucleotide sequence ID" value="XM_007376184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQC1"/>
    <dbReference type="STRING" id="619300.G3AQC1"/>
    <dbReference type="GeneID" id="18875167"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67527"/>
    <dbReference type="eggNOG" id="ENOG502S558">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019058_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQC1"/>
    <dbReference type="OMA" id="FNLHYVR"/>
    <dbReference type="OrthoDB" id="48651at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="73"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="204"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="358"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="494"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="359"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="376"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="433"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="534"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="568"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="616"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="642"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="701"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="712"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="748"/>
            <end position="758"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="758" mass="84169" checksum="946402930FDCB39F" modified="2011-11-16" version="1">MAPPKKMTLEEFFKDETYGGSWADADVDMSSISVPIEKYSSGFDSFSPRSGSGRASSSRGAAGGGGVAVGPPYIVKLLSIPMTANDGFIEDLFQSRYTPFVKFKILVDPSSDILETHIIRKVAFVELESSQEMAKVLKWYDLYYKGNRRVIVEPADFADFQYCIKFNQEHEREIQEIQDEFFANKQHRNQPRHMALLDQFDLDSHGHIHGRRPSRSPNRYEPPSRSPSGRYDGHSNRYPRRQSFEDLPPRRGSYSQQPPLAPLTPHSSSTTTEATPALTPKPNPFGAAKPVDTAHISTSTAPAKPKSNPFGAAKPVDTLSRQLEIEKKLINLNKTTVQTLGDDEKVDVEGVIKEFHESASPTLRRNSINTPPRRASYNEKKVTILKKDQSDEQPKSAPIKIEKPPQETKAQLPPTERPSPSGGSYVPNGTGKSLAELLSEGASSGTSTGGKNTPRNSTPKPVVAKPIILKKKVPTSPQPAKVDLTIKEGEYLKQQAGSQKIEPKQPLIPMEIPILVQPTPPQQPQKPQKSQKVQVEEKKVVMVTKKLEGFEITPEEKPATNGTSDQGAKTDDEDRPDFKKLSEIVQRNEEKRNQNLNKRGGGPQRKFRNSFSSGRGRLERNWREGDSSGRETSESINKSEEGNISNKFRSTPRSTSPTKPEPSNVHKPKTEEKEKAPVVIEESTAKETIEYNTLEKVTSNRSEEGSETRGRGGWRGRGRGRGRGRGGFRGRGPPRGHNLHYVRPKPGNEQSNNIKAEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQR5</accession>
    <name>G3AQR5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Phosphoribosylaminoimidazole carboxylase</fullName>
            <ecNumber evidence="4 11">4.1.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_62222</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + H(+) = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + CO2</text>
            <dbReference type="Rhea" id="RHEA:10792"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="EC" id="4.1.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">In the C-terminal section; belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.21" evidence="4 11"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376389.1">
        <property type="nucleotide sequence ID" value="XM_007376327.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQR5"/>
    <dbReference type="FunCoup" id="G3AQR5">
        <property type="interactions" value="185"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQR5"/>
    <dbReference type="GeneID" id="18874223"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62222"/>
    <dbReference type="eggNOG" id="KOG2835">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011534_2_1_1"/>
    <dbReference type="InParanoid" id="G3AQR5"/>
    <dbReference type="OMA" id="ITFDHEH"/>
    <dbReference type="OrthoDB" id="15425at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004638">
        <property type="term" value="F:phosphoribosylaminoimidazole carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1970:FF:000013">
        <property type="entry name" value="Phosphoribosylaminoimidazole carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000030">
        <property type="entry name" value="Phosphoribosylaminoimidazole carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000016">
        <property type="entry name" value="phosphoribosylaminoimidazole carboxylase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000037">
        <property type="entry name" value="Phosphoribosylaminoimidazole carboxylase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01928">
        <property type="entry name" value="PurK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016301">
        <property type="entry name" value="Ade2_fungi/plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003135">
        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005875">
        <property type="entry name" value="PurK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040686">
        <property type="entry name" value="PurK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054350">
        <property type="entry name" value="PurT/PurK_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004679">
        <property type="entry name" value="PRK06019.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01161">
        <property type="entry name" value="purK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609:SF5">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609">
        <property type="entry name" value="PURINE BIOSYNTHESIS PROTEIN 6/7, PUR6/7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02222">
        <property type="entry name" value="ATP-grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17769">
        <property type="entry name" value="PurK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22660">
        <property type="entry name" value="RS_preATP-grasp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001340">
        <property type="entry name" value="AIR_carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52255">
        <property type="entry name" value="N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="10 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="13">
        <location>
            <begin position="110"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW31611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="567" mass="62009" checksum="1E110074C8A2B2DC" modified="2011-11-16" version="1">MDGKTVGILGGGQLGRMIVEAAHRLNIKTVILDAPDSPAMQINALDEHVAGSFKEYESILKLAEKADVLTVEIEHVDVEALKKVKEKFPKIEIYPLPETIKLIQDKYVQKEHLIKHGIAVTESVAVESNTEEALLKIGEEYGYPFMLKSRTLAYDGRGNFVVKDKSYCKQALEFLKDRPLYAEKWCPFTLELAVMVVRSIQGEVFAYPTVETIHKNNICHLVHAPARVPDTVATKASIAAKNAVKSFPGCGIFGVEMFLLPDGEILVNEIAPRPHNSGHYTIDACVTSQFEAHVRAVTGLPMPQGFTEFSTTTTNAIMLNILGDKETANKELEICRRALETPHASVYLYGKTTRPERKMGHINIVSSSMSDADSRLSYILGETTEIPESIQSKEQPLVGIIMGSDSDLPVMAVGARILKQFGVPFELTIVSAHRTPHRMAQYAIEAPQRGLKCIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSTLDGVDSLHSIVQMPRGIPVATVAINNSTNAALLAIRILGAVDSKWLTKMSQYMLNMENEVLGKAETLEDIGYEDYLVEKLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQZ0</accession>
    <name>G3AQZ0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_141055</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM170 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376429.1">
        <property type="nucleotide sequence ID" value="XM_007376367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQZ0"/>
    <dbReference type="FunCoup" id="G3AQZ0">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQZ0"/>
    <dbReference type="GeneID" id="18870339"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141055"/>
    <dbReference type="eggNOG" id="ENOG502S0YM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071343_1_0_1"/>
    <dbReference type="InParanoid" id="G3AQZ0"/>
    <dbReference type="OMA" id="FPMQGGL"/>
    <dbReference type="OrthoDB" id="2131401at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071464">
        <property type="term" value="P:cellular response to hydrostatic pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019334">
        <property type="entry name" value="TMEM170A/B/YPR153W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22779">
        <property type="entry name" value="SD17342P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22779:SF6">
        <property type="entry name" value="SD17342P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10190">
        <property type="entry name" value="Tmemb_170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="155" mass="17084" checksum="EE8FF52CE7609C3F" modified="2011-11-16" version="1">MRLFISSLNIPIGYSTPSFPSLFWPLGPTRSAYQVALLYYTIDIWKFTVWWTLILFGGLYTIVGIIAAASSLLNKSRHNLNIGGVEIMMSFSIVFFYLIIGLTRGFCSGAVVGIVVAAIYNAGSLTMSTWIPMTWAIAQVLYDIASSYSTTSIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARD6</accession>
    <name>G3ARD6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_51724</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376521.1">
        <property type="nucleotide sequence ID" value="XM_007376459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARD6"/>
    <dbReference type="GeneID" id="18871586"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51724"/>
    <dbReference type="HOGENOM" id="CLU_474994_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARD6"/>
    <dbReference type="OrthoDB" id="4027973at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40903">
        <property type="entry name" value="GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40903:SF1">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442525" description="Hyphally-regulated cell wall protein N-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="299"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="377"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="415"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="453"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="496" mass="52861" checksum="C4B16F6A7BA45366" modified="2011-11-16" version="1">MKLLASLFLFLTSVIALDVSSTTTITSASVLGESYLIESTGVLELLNSIQLYLTSLTVRSLGQFFFLADSLTNIDIQTMDNSGYVLFDLPDSTYLPFRSNSRNSGEFVVNTGHSIVDFTDLDNTGSLTLWATFYTGPIAFFHGLVNTGNICLHEFVSNIENASGKGCYALSQSTVYIQSAASDFEPTVVFEDPSNGFYIVNTPQVLRLKLINFGNGNFIGFPNQGYITMNYFSGSGYLVVYIAIYEIYVNIGFGYDLGLFIASSNSEQVRIKYDGSVPSGANQGGCSCSLNIRTPPAPESTIELETSSEVSTSSSIEITSSSVVTSTSSSVAGSTYRSSTKSHSSSKSCPTPKPTNGHGNNGNGHDNNHGNGHDNNHGNNSNGNNHGNDNHNGNGNSNNHDNHDNGHGKEHNDGHTNGNGNNNHNDNHGNGNSNNNNNNHGNSKGKSNNNNGNGKGNDKGGGNGKGHKRRDVLDNSACSTHKSMLVLASLVFLAIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARF5</accession>
    <name>G3ARF5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="10">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_154183</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Exact function not known, phosphatase 2A performs an essential cellular function.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 10">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="10"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376054.1">
        <property type="nucleotide sequence ID" value="XM_007375992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARF5"/>
    <dbReference type="FunCoup" id="G3ARF5">
        <property type="interactions" value="1094"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARF5"/>
    <dbReference type="GeneID" id="18870973"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_154183"/>
    <dbReference type="eggNOG" id="KOG0371">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004962_8_1_1"/>
    <dbReference type="InParanoid" id="G3ARF5"/>
    <dbReference type="OMA" id="CMKVRYP"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="C:protein phosphatase type 2A complex"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990813">
        <property type="term" value="P:meiotic centromeric cohesion protection in anaphase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010972">
        <property type="term" value="P:negative regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010515">
        <property type="term" value="P:negative regulation of induction of conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000786">
        <property type="term" value="P:positive regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903432">
        <property type="term" value="P:regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000003">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033096">
        <property type="entry name" value="PPPtase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="12">
        <location>
            <begin position="168"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR033096-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="363" mass="40760" checksum="872F0A0B1E46C4F9" modified="2011-11-16" version="1">MELDTDVPMEDVEDLKQVSTSATSSSTSGKQPTGITSIGGDASVSDAAAAAKKADLNVSSVNQLDQWIDKLSQCEPLAEADVKKLCDMAIEVLQFEENVQPVQVPVTICGDVHGQFHDLMELFKIGGPCPDTNYLFMGDYVDRGYYSVETVSYLVCMKVRYPNRITILRGNHESRQITQVYGFYDECLRKYGSANVWKLFTDLFDYFPITALVDNKVFCLHGGLSPMIETIDQVRELNRIQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGFTFGQDISEQFNHTNDLSLIARAHQLVMEGFSWSHQESVVTIFSAPNYCYRCGNQAAIMEVDEQHNRQFLQYDPSQRPGEPTVTRKTPDYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARH0</accession>
    <name>G3ARH0_SPAPN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_61861</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007376007.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="G3ARH0">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARH0"/>
    <dbReference type="GeneID" id="18874084"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61861"/>
    <dbReference type="eggNOG" id="ENOG502RXQM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086134_1_0_1"/>
    <dbReference type="InParanoid" id="G3ARH0"/>
    <dbReference type="OMA" id="EFVPHAY"/>
    <dbReference type="OrthoDB" id="4064682at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2790">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="260" mass="29369" checksum="9E8C923F1757A910" modified="2011-11-16" version="1">MSQQDDTIEESLTMFFDNDFNPPAYIDKLVQSITVPTSALPTSPNLSSTTTTTTTTATPISAYSKNSLSKLSNDISQLIIHLDYYTNELSNNNLKHKLDALNKSNTLIYSRDDESTGSTTRLQYHINVLNNSILSLQAELNGINEKLDINHGKNEAINDLIQLKQVKANLTKVLTIFELVNNSLLRHDSYSVEQFQEALNELFDSIKQQLDSNVDQEKLLAHINKLIDLSSVFSNLAKFNPIFKKFISQLTAERDQFLKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARM6</accession>
    <name>G3ARM6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein RMD9, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_140469</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376557.1">
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    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="619300.G3ARM6"/>
    <dbReference type="GeneID" id="18870293"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140469"/>
    <dbReference type="eggNOG" id="ENOG502QUSW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="InParanoid" id="G3ARM6"/>
    <dbReference type="OMA" id="WENGVND"/>
    <dbReference type="OrthoDB" id="4081443at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="629" mass="72792" checksum="C45A26AC74326C17" modified="2011-11-16" version="1">MFKLITTNTQSSLRPALLQKIKNATANASQLSTSLSHTAISPVAATSPQIYPFTHQFVRNNSNVATKLNEPVNVPPPRIIPDVSKGGDLHEIKSQSEPSLLSEIDVKQKLDQFDQINLRTKYNGSHPFYLEELDTSLELLETKAVRDRLSEEQLTGFVYNIERMIFHQKRRRLRNNFNRDRDTQGAFQTEIMIQSAALKLFDMINAGSLTHCLSAEALYRLFLILHSFYLRSEIVSYWEAGVSNPDIANFFVDQKVLSTALFMAYEIKRFSYEEIQQIYDLNTPDKTNVFPDLLTSVGKVALLENDYDRALDLFEQLMISLERDHKNTSTAIAEMHLNFISMCREVDIAKHFFDKAYDSRKLPYTVKLKAPHMISFMRNCSEAGESMDEILQIWKKLLIKSMETAKYDPKGAKSANLNSGMFKLLFDKYPEYTEEAKKYLNKMFTMNPQINEIFINAAISNYSWQDRQTFDMLVDAYDKYGIPKTMMSNRIILKQAGLFDHSNEEIIEFWNNLLYSLDELKYSHIAVPDWAAIRDATILSEHYGDQRTGLYMSILKAYKDYMQNDFACVKFLRGFGRNAEHVRLLKKFTEGEPHFDNEVEITVPGDLHSLKKHIDFKDCAKRFYQDQPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS01</accession>
    <name>G3AS01_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62460</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376628.1">
        <property type="nucleotide sequence ID" value="XM_007376566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS01"/>
    <dbReference type="GeneID" id="18874328"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62460"/>
    <dbReference type="eggNOG" id="ENOG502T156">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058068_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS01"/>
    <dbReference type="OrthoDB" id="4013061at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="364" mass="40183" checksum="3C23A35C4B5C7A4A" modified="2011-11-16" version="1">MSVDSCNSETFDNNIGNLPNKNLTMTSKVLHTSGGTPSSSSIATTTNGVTNTNNIPILNDNHAPRDIRHTIQTHTVAASGTTAGAAAAAAATVLLPPDLTPSYPYDEPDEDEYNMHIGTQPPPRIYTDEHPEPELLPTTVGRDSQGRIIPSTSTISLLSLNQQPDMSMTSPKIIVSNPPVFIDRKNSFGQLRNLINHKRLQPYQKMNSPNEYTFMTTTTQSIPIQEEHVLQPPSPNLDPVSLGGSPSRFWLNSPTITKSLSKTQLQYTQNTQTLHSYQKQQQFHPLAPIPQSVELPMQQTDSELYINIQHRNGSHSPTLDPVQTPSEDPPMTPLSLNRAVNGYSTDYFNLDSVMQKNKEDEANY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS18</accession>
    <name>G3AS18_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_56614</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376645.1">
        <property type="nucleotide sequence ID" value="XM_007376583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS18"/>
    <dbReference type="STRING" id="619300.G3AS18"/>
    <dbReference type="GeneID" id="18872054"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56614"/>
    <dbReference type="eggNOG" id="KOG3067">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1981376_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS18"/>
    <dbReference type="OMA" id="LHYEINL"/>
    <dbReference type="OrthoDB" id="829at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.200">
        <property type="entry name" value="Translin, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016069">
        <property type="entry name" value="Translin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002848">
        <property type="entry name" value="Translin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036081">
        <property type="entry name" value="Translin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01997">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74784">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="126" mass="14269" checksum="D75431D5B7084139" modified="2011-11-16" version="1">MVGNHDSTGVIVNTNELPDLLNLEKVDYQVYLSALIRLIDTMVEYTNGTVIRGSIQSKGPYGKDYSIAMINLEIVLKLEKGFQLLDLNNENLRKLYDSLKLNCQKLSKMTYDLSLRNLINVKGETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS80</accession>
    <name>G3AS80_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_56956</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377072.1">
        <property type="nucleotide sequence ID" value="XM_007377010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS80"/>
    <dbReference type="STRING" id="619300.G3AS80"/>
    <dbReference type="GeneID" id="18872085"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56956"/>
    <dbReference type="eggNOG" id="ENOG502S5A2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136352_2_0_1"/>
    <dbReference type="InParanoid" id="G3AS80"/>
    <dbReference type="OMA" id="YRIANNV"/>
    <dbReference type="OrthoDB" id="5331396at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37849">
        <property type="entry name" value="YALI0E11605P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37849:SF1">
        <property type="entry name" value="YALI0E11605P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="89" mass="9710" checksum="42567E252DF1FF2A" modified="2011-11-16" version="1">MLSISRHLARSTVRSYSTVAATSTPKKVGAFRGGFVGFLLGVTATGVVSYYYLLDQYKLANTVIVADIVSLQNSISSLEKHIKNLEEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASI7</accession>
    <name>G3ASI7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="15">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">SPAPADRAFT_63031</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="15">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="15"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377138.1">
        <property type="nucleotide sequence ID" value="XM_007377076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASI7"/>
    <dbReference type="FunCoup" id="G3ASI7">
        <property type="interactions" value="1248"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASI7"/>
    <dbReference type="GeneID" id="18874562"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63031"/>
    <dbReference type="eggNOG" id="KOG0342">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_26_5_1"/>
    <dbReference type="InParanoid" id="G3ASI7"/>
    <dbReference type="OMA" id="LMEFHSQ"/>
    <dbReference type="OrthoDB" id="10259640at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990417">
        <property type="term" value="P:snoRNA release from pre-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17942">
        <property type="entry name" value="DEADc_DDX18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000379">
        <property type="entry name" value="RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000460">
        <property type="entry name" value="RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044773">
        <property type="entry name" value="DDX18/Has1_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14 20" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="19">
        <location>
            <begin position="96"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="127"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="317"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="13">
        <location>
            <begin position="96"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="58"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EGW31105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="560" mass="62333" checksum="7D454E24E204AEC5" modified="2011-11-16" version="1">MAKNSKQSKPKHSAKESKKRAHAEVEQDSNSPGEEESEKLTAEVDADFDEVANLLGQDIKDPDAKRKSKAVEEPAIPEYTPVESSDKSKEENEEDVFFKDAELSEPTMKAITEMGFTKMTKVQAKTIPPLLAGRDVLGAAKTGSGKTLAFLIPAIELLHSLKIKPRNGTAVIIITPTRELALQIFGVARQLMEHHSQTCGIVIGGADRRAEAVKLGKGVNLLVATPGRLLDHLKNTPGFVFNNLKALVIDEADRILEIGFEDEMKQIIKILPNENRQSMLFSATQTTKVEDLARISLRPGPLYINVVPETDVSTADGLEQGYVVCDSDKRFLLLFSFLKRNIKKKIIVFLSSCNSVKFYSELLNYIDLPVLDLHGKQKQQKRTNTFFEFCNAKQGILVCTDVAARGLDIPAVDWIIQFDPPDDPRDYIHRVGRTARGTQGKGKSLMFLTPSELGFLRYLKAANVPLNEFEFPSNKIANIQSQLVKLIKTNYLLHQSAKDGYRAYLQAYASHSLKTVYQIDKLDLVKVGKSFGFEVPPKVNITIGASGKSIEKKHKKQRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASU7</accession>
    <name>G3ASU7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_68356</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377194.1">
        <property type="nucleotide sequence ID" value="XM_007377132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASU7"/>
    <dbReference type="FunCoup" id="G3ASU7">
        <property type="interactions" value="757"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASU7"/>
    <dbReference type="GeneID" id="18875212"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68356"/>
    <dbReference type="eggNOG" id="KOG0922">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001832_2_4_1"/>
    <dbReference type="InParanoid" id="G3ASU7"/>
    <dbReference type="OMA" id="MKEVDQV"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071021">
        <property type="term" value="C:U2-type post-spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000386">
        <property type="term" value="F:second spliceosomal transesterification activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="S1_DHX8_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000001">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000615">
        <property type="entry name" value="pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049621">
        <property type="entry name" value="S1_DHX8_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF85">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="141"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="412"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="602"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="212"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="228"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW31161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1059" mass="120601" checksum="E7E74165F8C3C6CC" modified="2011-11-16" version="1">MSSHTLNTNDKDLLDSISKLVTSHLKVNDDNFTITKFLLNLYKQTLNTSKPGRLIEFKRIVKDNGGDGFPAEFINQTFNCIESLNPGKQESAHLPVLHLQNRIEDKNDRPTDDSRSVRHEHERKPRYDIRRELHDLPLQVGQIYRGYVSEITPYGAFIRLRSPNSYQSGLCHISQISDTRIHSVNDVLSLDQQVFVKITEIKEQQRRQRISLSMKGIDQDTGLEDPEANGKERGRSKEVKKPIKRKLTSPERWELRQLISSGTVSIEDYPELAEEENTEFKHEPEIEVDIVLNPTQPKFLKGQKLKYELPTLENVSQPAGSLKKIAEKGSELAKEFRQEKLKETKEATKDQDVFDPLFQEVSESDESTNKRETFISQWKKSQKNVKYGKITSLPIQKQRQQLPIYSMRSELVEQIQNNQFLVIVGETGSGKTTQIVQYIKEEEINKTLDGKTKIIGCTQPRRVAAQSVAKRVSEEIGCKLGEEVGYTVRFDDNTSSSTVIKYMTDGMLQREALNDPSMSKYSVIMLDEAHERTIATDVLFALLKKAAAKNPDLKVIVTSATLDSGKFSAFFNNCPIVKIPGRTYPVEILYTKEPETDYLAAALDSVMQIHLSEPAGDILVFLTGQEEIDTSCEVLFQRMKILGDSVPELIILPVYSALPSEVQSKIFEPTPAGSRKVVLATNIAETSITIDGIYYVIDPGFVKINAYDPKLGMDSLTIHPISQAQANQRSGRAGRTGPGKCYRLYTEQAYNKEMIANTVPEIQRTNLSHTILMLKAMGINDLLTFEFMDPPSNNTMLVALQDLYTLDALDEEGYLTQLGKKMADFPMEPALAKTLIKSVEFECTEEILTIVAMLSVQTIFYRPKEHQKLADQRKLRFHHPLGDHLTLLNVFQSWQLNGCSKVWCQDNFIQERSMKRAMDVRKQLKSIMTKYGYRLTSCGSNIDLIRKTLCAGYFKNTSKRIANEGYKTLAEETAVHLHPSSCLFGKNPEYVLFHSLLLTTREYMHCVTVIEPKWLYELAPKFFRQGEGENKKQEKIVPLFKGNKDEWRLSTQKKRNLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASV9</accession>
    <name>G3ASV9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62598</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376773.1">
        <property type="nucleotide sequence ID" value="XM_007376711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASV9"/>
    <dbReference type="STRING" id="619300.G3ASV9"/>
    <dbReference type="GeneID" id="18874396"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62598"/>
    <dbReference type="eggNOG" id="KOG2044">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1066231_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASV9"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="247"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="261" mass="30089" checksum="765D200006608739" modified="2011-11-16" version="1">MPNIDNKEFLQVTYEMPPKKLGKSMLLNGYIPHLPALTEEDKSNLMYGGPRYGGGRFNAPSDNREYINKGPAGTEIYKAYSMRRGGYRSYLQYLKDGVLPDSGTRQGGYRTQNYDNNQGYNRNYNQGYNNSQGYHNQGYSSNQGYNNQGYNNQGYNNQGYNNNQGYNQNYNQGYQGQGYNQNYNQGNHRQQGHGYNQNYNQGYNQGYGQGRQDYGQGRQDYGQGRQDYGQGRQDYNKQSRSGYLPPNSNRGNYRGRNSYRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT36</accession>
    <name>G3AT36_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62668</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376832.1">
        <property type="nucleotide sequence ID" value="XM_007376770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT36"/>
    <dbReference type="GeneID" id="18874423"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62668"/>
    <dbReference type="eggNOG" id="ENOG502RQ9G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118184_0_0_1"/>
    <dbReference type="InParanoid" id="G3AT36"/>
    <dbReference type="OMA" id="WLEHYEE"/>
    <dbReference type="OrthoDB" id="4020157at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023378">
        <property type="entry name" value="YheA/YmcA-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158622">
        <property type="entry name" value="YheA/YmcA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="197" mass="22994" checksum="2627A5053D2525CE" modified="2011-11-16" version="1">MVSPSSSPTQSTGTHKIIGAKSAGHTDNLRSSPPRSILEDIQQIQHDRSMKLLHPLSLSPIRAKEPERFQSHSLSSPLRKSSEPRNVKTINSRNKHDIMQKTRQQYVQEKLLLQRDKQSSQQFTQDLIRKYEEEYNQIMQGVDIDQLIEEEQNVNYADDESWEDEYQQELEELEFEEDLELVELLHSLELNEQASDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT57</accession>
    <name>G3AT57_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 14">Phosphatidylinositol 3-kinase VPS34</fullName>
            <ecNumber evidence="4 14">2.7.1.137</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_142747</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Multifunctional phosphatidylinositol 3-kinase involved in acidification of vacuoles, pH-dependent cell growth, and autophagocytosis. Plays an important role in protein transport and virulence. Component of the autophagy-specific VPS34 PI3-kinase complex I essential to recruit the ATG8-phosphatidylinositol conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal structure. Also involved in endosome-to-Golgi retrograde transport as part of the VPS34 PI3-kinase complex II. This second complex is required for the endosome-to-Golgi retrieval of PEP1 and KEX2, and the recruitment of VPS5 and VPS7, two components of the retromer complex, to endosomal membranes (probably through the synthesis of a specific pool of phosphatidylinositol 3-phosphate recruiting the retromer to the endosomes). Finally, it might also be involved in ethanol tolerance and cell wall integrity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12709"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.137"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:12710"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the autophagy-specific VPS34 PI3-kinase complex I composed of at least VPS15, VPS30, VPS34, and of the VPS34 PI3-kinase complex II composed of VPS15, VPS30, VPS34 and VPS38. Interacts with VMNA7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.137" evidence="4 14"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376853.1">
        <property type="nucleotide sequence ID" value="XM_007376791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT57"/>
    <dbReference type="FunCoup" id="G3AT57">
        <property type="interactions" value="847"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AT57"/>
    <dbReference type="GeneID" id="18870480"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142747"/>
    <dbReference type="eggNOG" id="KOG0906">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004869_0_0_1"/>
    <dbReference type="InParanoid" id="G3AT57"/>
    <dbReference type="OMA" id="LHKFAQY"/>
    <dbReference type="OrthoDB" id="67688at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071561">
        <property type="term" value="C:nucleus-vacuole junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032120">
        <property type="term" value="P:ascospore-type prospore membrane formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051365">
        <property type="term" value="P:cellular response to potassium ion starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000425">
        <property type="term" value="P:pexophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08397">
        <property type="entry name" value="C2_PI3K_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00896">
        <property type="entry name" value="PI3Kc_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 3-kinase catalytic subunit type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 3-kinase catalytic subunit type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ARM-type_fold"/>
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        <property type="entry name" value="C2_domain_sf"/>
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    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR057756">
        <property type="entry name" value="PI3-kinase_type3/VPS34_cat"/>
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    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
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    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
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    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
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    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
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    <dbReference type="InterPro" id="IPR002420">
        <property type="entry name" value="PI3K-type_C2_dom"/>
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    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
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    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
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    <dbReference type="InterPro" id="IPR008290">
        <property type="entry name" value="PI3K_Vps34"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF7">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE CATALYTIC SUBUNIT TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00792">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000587">
        <property type="entry name" value="PI3K_Vps34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00142">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51547">
        <property type="entry name" value="C2_PI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="8 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="C2 PI3K-type" evidence="18">
        <location>
            <begin position="50"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="PIK helical" evidence="17">
        <location>
            <begin position="322"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="16">
        <location>
            <begin position="668"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="784"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="819"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="787"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004150"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012073"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
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            <dbReference type="ARBA" id="ARBA00023985"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
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            <dbReference type="ARBA" id="ARBA00041128"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00059175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW30820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1032" mass="118530" checksum="6151571A00E6721B" modified="2011-11-16" version="1">MTATTEAHDALTQVKLSNTFGLSKDLTDPIIVKICYLEPTLITQHESIPLSTKFENPSVFKTSSRIYKNSDLFIRIGMYDGKNNNLIAPVSQTTFTHFRGRRVWNQYVKLAMEYNQICSGAYLKFVIYEIVDCKSVVFGVGYLSMFNHTRSTLRSGSQKVAINTKLPDKPSVDYGDPKGLTELETNLIKFENGEFNRVNWLDKLVLPKLTGGQKSDQEEINTDADYFLYVELPNFEFPIVYSDIKYEIPTIPKFEPKQPENSSIVINSVDISSEGSQPKVYDPDFQIATRLNHINATTTIYDPIELKYHKLERNINNNTMLDKELKPTPQLRDELLRILVKPSNIELNDYEKNLIWKFRYYFSKNNQTNIDSRNKTTTQSFLPKFLKSIDWDNDYEVDHAFNEIIPNYWGVNNLQIGDALELLGDYFNPFVLGRSMYPVEQPQRDSSKLKDDEKRFLNLFKNVTLLRKLAVDRLRLASSDELLLYLLQLVQALKYESLIYERKLPFDEIDLHHHKNNGDPLESPLAKFLIDESVHSEKLGNFFYWYVKVENEDQLNTRSTPPQERSPFENTDSKMYGAILNKYIDNLKKHCHEHRLPYYKHLKRQIWFIKKLTGLVELLRSSFRKNESTMRKTEFLKEYLSNSSNELLKFPDPFPLPLDPSIMICGCYPEESAVFKSSLAPLKITLKTIESGGASRHSATSQIIFGKKHNKYGKYPMMFKIGDDLRQDQLVIQIIDLMDQLLKNENLDLKLTPYKILATSPVAGLIQFVPNETLDSILSKTYPEISRPTSNQSSANSGAPQVSTPTTTNGILNYLQVHSRDQQESEPTPTHSILGASHVAAPTSPPSPRVQPKPAITSNMGVSPVLMDNYVKSCAGYCVITYILGVGDRHLDNLLLSPNGKFWHADFGYILGRDPKPFPPLMKLPIQVIHGMGGLQHENYNIFKSYCFITYTTLRKNSNLILNLFQLMLDANIPDIKFDRTRAIEKVQDKFCLQMTEEEAILHFQNLINDSVSAFMPVVIDRLHSLAQYWRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATG5</accession>
    <name>G3ATG5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_52118</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376961.1">
        <property type="nucleotide sequence ID" value="XM_007376899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATG5"/>
    <dbReference type="STRING" id="619300.G3ATG5"/>
    <dbReference type="GeneID" id="18871629"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52118"/>
    <dbReference type="eggNOG" id="ENOG502QVXD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_286370_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATG5"/>
    <dbReference type="OMA" id="QINLRRQ"/>
    <dbReference type="OrthoDB" id="1938591at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12191">
        <property type="entry name" value="gal11_coact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033789">
        <property type="entry name" value="Gal11_coact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036546">
        <property type="entry name" value="MED15_KIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008626">
        <property type="entry name" value="Mediator_Med15_fun"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16987">
        <property type="entry name" value="KIX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05397">
        <property type="entry name" value="Med15_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mediator complex subunit 15 KIX" evidence="7">
        <location>
            <begin position="20"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="114"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="237"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="292"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="413"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="668"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="811"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="630"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="114"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="297"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="336"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="413"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="471"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="668"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="739"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="765"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="811"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="837"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="868"/>
            <end position="902"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1081" mass="121304" checksum="0B3140FB5CB23784" modified="2011-11-16" version="1">MSNWQSPNIMSMMQPVNKNIGVTLEDRQKVIQVVLATFTRTNFPGDPPRLAKSACDFEGWIMNRAANKEEYGKLVHMKLEQIKQKFQNQQQQQQQQQQLQFMQQQAQARAQPQQQAQVQARQQQQQQLRDMPNMNQQQVPPQIQQQQQNQQQQPPQQQHQTIDQLVRSTPIPQVVLAKLPNLPPGITRWQQIMELIQKKKMPEAYLPIIKDMYSAHTQLVLHDQQKMNQMQRNVTNDSNNTISSSPPTMNPTNTTSTTTNNNNNNMMMNTYASAGLNNLTPQQREQLMQRQLNKRNMQQQQMQQQQQQHQPPMQQQQSPQLMQQIPDSASVPPIPQQQQPPQQQIKQPQIQITQQDYANYSAKALKMLQEIEARGQLNQPLTPAMKESFVRRFIIRQKQNLLQQQQQQQQQQQQQQAQNQQHPQQIAQQQHPSVPLQQPQPMQPMLQQGQPQQSQQQSPMLTNIKTPMMKPNQQTPMSNTLSSPLMPQQRGPTAPVGPTPVQSAPTSGIAPLTEEAKLQLKQLFTEVASKEVMLKDVTNMLTPEQKERVRTLLNEIQIQFPNIDAVISYVYVLTRNIEVTKRLIQMKFTIKKILDNLRSGIYITNPEMLTKLSMQLAKYFGYVRDQVALRKQQQGQRAQVQAQAQQQQQQAQAQQRQVPPQQTPLMQNQMNVQQQQQQQQQQPGQFMGQNQQIPPQQQVNMGNSNSWNGAGGPGASGPVKTFPMQGAVPRQNISGSPLMPQVPVPMQMPVSVPQQTIPQPPPSGVPMSQQQQQQQQQQQIPTPQQMAGQPLAKARQPSKVGLAAAAAAAAAASQASTPAAAKKTVGGQGRKKKQSISAAPTPAAASSGSTAPMTAPTPTPGSLANAIKTPNYNIATPQIPQSQSNKNTPAGQSPNVRATTNNTVSTPVIVDASVDLFAMIQSDPKVARRKELSNSDPVKFFYASLANLLELDENIVDGSAKKGRSPLSPVMTTLTSANGKGWTCEIKPQAITGSFRQVGLIRELVDDDDIIGRCEELALGEVGVKREFESEDLDMLFTDKKPRLDDHIKEESNGGLMDDYKYVYEPVGFDEWKDFIISSIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU09</accession>
    <name>G3AU09_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_63232</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377356.1">
        <property type="nucleotide sequence ID" value="XM_007377294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU09"/>
    <dbReference type="FunCoup" id="G3AU09">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU09"/>
    <dbReference type="GeneID" id="18874654"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63232"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045870_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU09"/>
    <dbReference type="OMA" id="WDSARGS"/>
    <dbReference type="OrthoDB" id="48651at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043024">
        <property type="term" value="F:ribosomal small subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033592">
        <property type="term" value="F:RNA strand annealing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034057">
        <property type="term" value="F:RNA strand-exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097010">
        <property type="term" value="P:eukaryotic translation initiation factor 4F complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF11">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="77"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="33"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="162"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="34"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="237"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="344"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="369"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="380"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="420" mass="46971" checksum="A7EB7C73F25EEEC4" modified="2011-11-16" version="1">MDIGSFLADESLGGSSWADEEVDLTSIEATFNKSSTAAPIGGGSSAASSGWGASETGSSGFEKRERKEFPIPDQAPFRARVGNLPWDGIAEDDVVRYFEGRMQAQDIISDVKLPTDRETGKLRGFAFVTFNERAMLEESLNLNLSEFNGRRIFVNVAAPLKGGEDGDWKRSGPLGGRREEPNLDWGSARNSQAALPPRERSFRERGDRPERSERPPRKEEPDLDWGSARTSHVSLPPRERSFRGERGDRGDRPERTPKKEEPDLDWGAARTSHATLPPRERSFRGERSERPPRKEEPEFDWGSARSGHGTLPPRERSNRHKKEEPELDWKRGQALQPRTPKSNKKAEEKKASDQPKPQQSSYSVLAGLEGDDDSEEEEQQQQQQQQKPVEQEVAQLESATANLSVSEAKEDAEGWEVVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU41</accession>
    <name>G3AU41_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vacuolar protein sorting-associated protein 68</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_57203</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0220 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377388.1">
        <property type="nucleotide sequence ID" value="XM_007377326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU41"/>
    <dbReference type="FunCoup" id="G3AU41">
        <property type="interactions" value="202"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU41"/>
    <dbReference type="GeneID" id="18872106"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57203"/>
    <dbReference type="eggNOG" id="KOG3393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096876_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU41"/>
    <dbReference type="OMA" id="VHITFVD"/>
    <dbReference type="OrthoDB" id="268928at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034424">
        <property type="term" value="C:Vps55/Vps68 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007919">
        <property type="entry name" value="UPF0220"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13180">
        <property type="entry name" value="SMALL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05255">
        <property type="entry name" value="UPF0220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="19538" checksum="F683488AD6A914A0" modified="2011-11-16" version="1">MDHSNHLFRFNASMLRLPKSAKLRAFGVYLSGALFAIGFWSIIDSAVYSKSVNASDVHVTFIDWIPFICSTSGMLIVNSIEKSNLFQDSNTTNFMSGGNTNAWAARVILFLGFSLLAGGLAGSFMVFIIKYLLKHYTFPTLGMGVSNIVCNACIMLSCIVLWLSQNIEDEYSYSLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU77</accession>
    <name>G3AU77_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_143258</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377424.1">
        <property type="nucleotide sequence ID" value="XM_007377362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU77"/>
    <dbReference type="FunCoup" id="G3AU77">
        <property type="interactions" value="271"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU77"/>
    <dbReference type="GeneID" id="18870529"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143258"/>
    <dbReference type="eggNOG" id="KOG1245">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002631_1_1_1"/>
    <dbReference type="InParanoid" id="G3AU77"/>
    <dbReference type="OMA" id="FVEVHCA"/>
    <dbReference type="OrthoDB" id="332390at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018501">
        <property type="entry name" value="DDT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028942">
        <property type="entry name" value="WHIM1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028941">
        <property type="entry name" value="WHIM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013136">
        <property type="entry name" value="WSTF_Acf1_Cbp146"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32075">
        <property type="entry name" value="ISWI CHROMATIN-REMODELING COMPLEX SUBUNIT YPL216W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32075:SF6">
        <property type="entry name" value="ISWI CHROMATIN-REMODELING COMPLEX SUBUNIT YPL216W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02791">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10537">
        <property type="entry name" value="WAC_Acf1_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15612">
        <property type="entry name" value="WHIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15613">
        <property type="entry name" value="WSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51136">
        <property type="entry name" value="WAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WAC" evidence="7">
        <location>
            <begin position="23"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="135"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="268"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="473"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="811"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="734"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="500"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="533"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="543"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="819"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1172" mass="136917" checksum="D543A417D208A7DB" modified="2011-11-16" version="1">MVLYKRKQVTFIPPPAIPSDLSTQVWFIPQTKEWFITYDEYLARMDYYHKRKFVCEITGNSCLTFFEAYESELKEIKGVESNFPESLREHILRFLQFNRITRLDQLVDKVYSVFKNDYFPGETIFIKGIEDSKEQQQQQLQSQSTPPTSTSSSSSSSKQRGIIREKVQYGQSNQTKYLVVRLNDMQQAIVTNDNISRDRNHFTKWLIKTFIKLTMSRSHKVGAPWVVKDKYAKKYGIPQVYPEDLRHYADSTPTGEILYVTEKKKRRVPIKEDTHEPSATPVPEKRKKQIKTATPTVEVSTPTPAVVPFRKKFPTHYLPEKLQKELELEELKEQPSFGLSSLQPTKRNIVDDLQLRFDLQNSKPLPRQLHLPPNAQSLNNSLIEKYADQDIPNTSEIFRLSTNAISSIQDALQSWIFINIYHKVLNLDTFTFDDFIYCMGWNWEQYDELGRCELLDEIWCACLSAIVSNELPTRKEQRQAEDDGEIFGLNVQLPPKKGDESEDVTGSETDDEDKLLKDEPETDEEDTENGVVETKEVVIVKDQDEDNDEDEEENEADNEEEPEEEAPEYITSEGSDTDDSTPHNAYICMNYRNIPWHERLRKRNFKDGNWQCILLGVLSLVEHVPHYKTIIEQAYLKLAPKEKPATSSTVVNQFYQELDIDLKLQILNILIDLIITSPLVRNYIDDCLEQSSQLRRMRLDNIKDYKANLEIAQKANQFICNALNPGEEDKKPRLNYQNLEMTEAEQQKAEKDKEFAEQCEARKQALIKLNQIKKEKHSIEQKLTELDCQRVKLLGKDRLFNRYWWFENNGLPNLHAGNNDDDDDNDDDDEKEKDKEDVEDDDEKDEVSDETYLMGRLWVQGPSNDDLRIRYNSNFEESEKFNQELRRKEFDFTHKPEENGIKQEIKQEPVDEKKSQIREMDFIPISPAYKSTVQTIHHIEITQHEVHKNSDIVIDHEGSLVSPLALLTPFERKIIEELPDPLINGTNWRYYDHPEDIVKILSWLNPWGKRESALRKELTLVKDVVISNIEARRKALWMDGTPAEEDEIENNINKLKTKREKLIKGEDEDEIEETDDDVVVGAKRNLRRKQTPRKRQKVVTLEDALQFGNEEEITSMVEKLDSELAEKRQDRDLNRVVEWVNSIALDSFGKSLYEGGDKYKSSKTRKVTRKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUH9</accession>
    <name>G3AUH9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">pantothenate kinase</fullName>
            <ecNumber evidence="4">2.7.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_63364</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantothenate + ATP = (R)-4'-phosphopantothenate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16373"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the type II pantothenate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.33" evidence="4"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377506.1">
        <property type="nucleotide sequence ID" value="XM_007377444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUH9"/>
    <dbReference type="FunCoup" id="G3AUH9">
        <property type="interactions" value="778"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUH9"/>
    <dbReference type="GeneID" id="18874719"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63364"/>
    <dbReference type="eggNOG" id="KOG2201">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011154_3_0_1"/>
    <dbReference type="InParanoid" id="G3AUH9"/>
    <dbReference type="OMA" id="FKNPDIC"/>
    <dbReference type="OrthoDB" id="498611at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004594">
        <property type="term" value="F:pantothenate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008204">
        <property type="term" value="P:ergosterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000025">
        <property type="entry name" value="pantothenate kinase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004567">
        <property type="entry name" value="Type_II_PanK"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00555">
        <property type="entry name" value="panK_eukar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280:SF20">
        <property type="entry name" value="4'-PHOSPHOPANTETHEINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280">
        <property type="entry name" value="PANTOTHENATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03630">
        <property type="entry name" value="Fumble"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW30535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="307" mass="34562" checksum="853BD815326CAE1A" modified="2011-11-16" version="1">MGGGKLHFRDFQTENFKHEAMKFIIKLIQKSIDKSNNNPPITYIMATGGGAHKFYNLMTKTFRKYKLPMKIIAKDEMECLIRGLDWLITKIPNEIFTYDLIADHTKFTDLQSESSIYPYLLVNIGSGVSMVKVTGPGEHERIGGSTLGGGTLWGLLSLLTDAKDYNEMLEMAQRGNNENIDLLVGDIYGTNYNKIGLKAHNIASSFAKVFKKLRFTNKKPEDIARSLLYSISNNIGQIAYLQALRFNLKRIYFGGSYISGHMQTIHTLSYAVNFWSKGDMQSYFLRHEGYLGSVGAFIMGPASNNSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUL8</accession>
    <name>G3AUL8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_68654</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377545.1">
        <property type="nucleotide sequence ID" value="XM_007377483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUL8"/>
    <dbReference type="GeneID" id="18875229"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68654"/>
    <dbReference type="HOGENOM" id="CLU_1741718_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUL8"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="150" mass="17677" checksum="FDEBE09BA5AA754A" modified="2011-11-16" version="1">MAFVFNHEQLMNLDEKSRGEFLQIQSDIQELEQAVIERLRHFPDPHLFGTNCPLKEKINNLKKDERNQRIRINGIILKNKLMSGFKWNEKNKHTDEANDDEKISDEDIEYEFTPESNGSPLSKVVSWGKYLDIKRARSRDRSIATEFSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUP7</accession>
    <name>G3AUP7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_52671</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377574.1">
        <property type="nucleotide sequence ID" value="XM_007377512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUP7"/>
    <dbReference type="GeneID" id="18871704"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52671"/>
    <dbReference type="HOGENOM" id="CLU_057903_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUP7"/>
    <dbReference type="OrthoDB" id="4016968at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="318" mass="37019" checksum="55480550B8E33847" modified="2011-11-16" version="1">MVFRVNGLLFDIVKTQYPWIEVIAEDQNGYYHSQYPLQEYHYTQLILSRRARIPFLNGCQEMPWVKKLTIYLYFVSEQSLNEIKYLSGLEDLGIDIGDIDLLQELCKIGLGDIPIKKLRISVLELIHVEELQKIIEIFDLEKITAFQLHLWDVDTYPENFSLGIQELLSALVNVTSLSIICQNIDFGKVLESVKPNSLHTLHLKIVQVGDDIQNFQLENLFNNQEHSLQQFYLSNKKPQGGGRFYGLQEFERIYKKETKPLIPNQQPVGIFEIRRMIDDGKFQKLNRVLINESCYFIKRLSNGMIHLVPCNSLIPDNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUR5</accession>
    <name>G3AUR5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_157892</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377592.1">
        <property type="nucleotide sequence ID" value="XM_007377530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUR5"/>
    <dbReference type="FunCoup" id="G3AUR5">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUR5"/>
    <dbReference type="GeneID" id="18871104"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157892"/>
    <dbReference type="eggNOG" id="ENOG502S3HW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009086_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUR5"/>
    <dbReference type="OMA" id="CHNNTST"/>
    <dbReference type="OrthoDB" id="4068815at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014839">
        <property type="entry name" value="Crt10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08728">
        <property type="entry name" value="CRT10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="653" mass="75089" checksum="0995C0B123CAFF2E" modified="2011-11-16" version="1">MQIDRDYEYVLIKEGQQQATTEHINFFKYEFQPEYIRDKDPTQPSYGVKSNNINFSTIESSDPSTLVQNAPAEKFYNALNLKYLGIKKLYTVEIMSKYKNNLTSIIKDGHTGYEYLVAGSNSELVIFQFDHVSQLPDKRAVILFDTKPSYTSSTDRLISTWPYFPHSLNFIKTFDNFNGKQILSACSDDGTLLIWYTDTIIAYINKFKDVKSVDLDDTYRDENRFHGLKVKPDFKIKMEASLWGLDFINYKDEQGESHNLIVASDNSQSIGLFYYHAQDERLYHVKSHHILHNIPEVSFVDVKFDGTTHIVDVACASISGELIVFEFMFKLCHGPLDQDEFEYFKNEQLYYVDSKMEQLENRNGIDPDSLQFQHLKSKRFNRIIWSTPTVISRCVLNEDCWTIKPVDKKWFLPVVSFAAVFGDESINEHQQNNNISHESKLLGTSYDGTLSSNLGIASNFQFFESKVASLAPKPPHDVSVDSVKLTLIDDEYRRIHKEYVSKQPPNKFLVISTSKKLGMLHYPSLYCNCATRRIFDLPIPFHERSKFSNRISITEVIPKLSCLIAVSQQGLVTIMRLCCHNGVYGMRQEYLFPNALSLALGYHGYRTITGLAIRDKSLGSTPRFLIYLSYSDGIIVGYQLTLNDIDDIDIEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUY4</accession>
    <name>G3AUY4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_63693</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377841.1">
        <property type="nucleotide sequence ID" value="XM_007377779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUY4"/>
    <dbReference type="FunCoup" id="G3AUY4">
        <property type="interactions" value="817"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUY4"/>
    <dbReference type="GeneID" id="18874871"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63693"/>
    <dbReference type="eggNOG" id="KOG2345">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_109_1_1"/>
    <dbReference type="InParanoid" id="G3AUY4"/>
    <dbReference type="OMA" id="AMHQYKV"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032889">
        <property type="term" value="P:regulation of vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006624">
        <property type="term" value="P:vacuolar protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052239">
        <property type="entry name" value="Ser/Thr-specific_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45998">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45998:SF2">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="24"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW30075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="353" mass="39459" checksum="F9D3910FA45E0000" modified="2011-11-16" version="1">MNILACCFPCLPTSPTLSLNSTSYKIHRLLGEGGFSYVYLVSPTSNPNSFYALKKIRCPFGTGDESYKNAMREIHNYHRFTNSQTPYIIQTIDECIISEVDGGQSVYILLPYFQSSLQDVITKNVLNDEKMPESEIIRIFTGVCRGLKVMHNYQATSTTNTTTDDDNEQGQLLPDNDEDTLAGIDNDPESTQLTELTPYAHHDIKPANVMVSPEGLPVLVDLGSCTKARFNIRSRQQALALTDFAQEHCTLPYRAPELLDVSTNSTITEATDIWSMGCLLYSLCFGFSPFEKMELEQGANLNIAISQGKYIIPQGHEYSQELIKLIDSCLQVKPENRPTVAELLETTLQLARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV92</accession>
    <name>G3AV92_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor ISY1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pre-mRNA-splicing factor isy1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_144469</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ISY1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377661.1">
        <property type="nucleotide sequence ID" value="XM_007377599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV92"/>
    <dbReference type="FunCoup" id="G3AV92">
        <property type="interactions" value="228"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV92"/>
    <dbReference type="GeneID" id="18870629"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144469"/>
    <dbReference type="eggNOG" id="KOG3068">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043453_2_1_1"/>
    <dbReference type="InParanoid" id="G3AV92"/>
    <dbReference type="OMA" id="YHWERRI"/>
    <dbReference type="OrthoDB" id="1739576at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071020">
        <property type="term" value="C:post-spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071008">
        <property type="term" value="C:U2-type post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000384">
        <property type="term" value="F:first spliceosomal transesterification activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000389">
        <property type="term" value="P:mRNA 3'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.660:FF:000001">
        <property type="entry name" value="pre-mRNA-splicing factor ISY1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009360">
        <property type="entry name" value="Isy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037200">
        <property type="entry name" value="Isy1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13021">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ISY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06246">
        <property type="entry name" value="Isy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140102">
        <property type="entry name" value="ISY1 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW29895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="238" mass="28095" checksum="C9D899B909746072" modified="2011-11-16" version="1">MSRNKEKAQSSLNRFHQYKAQEAGVLISDPTLRPKYVQKVTSLPQAEKWRTTVISEISTKLTRIQDSTLGEFTIREINDDLNKLFKEKRAWEYHIRDLGGNDYLNVRDLTSSGINVKGYRYFGRAKELDDVKKLIEAQKREPGKGRAKEDEKKQLQNEERARESRIDYSYYGYFDEVNRTDDPLLKFEQQRSMELAKPQKEGDDDMYNFDQFPTNEQVSQWLVDKKRQQLLARLGIQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV95</accession>
    <name>G3AV95_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63515</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377664.1">
        <property type="nucleotide sequence ID" value="XM_007377602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV95"/>
    <dbReference type="GeneID" id="18874790"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63515"/>
    <dbReference type="eggNOG" id="ENOG502RQKR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1387247_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV95"/>
    <dbReference type="OrthoDB" id="10627089at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW29898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="197" mass="21635" checksum="2EFDC5B50041A5FC" modified="2011-11-16" version="1" fragment="single">MLNYNYSLNQAQLKTSSVTPTSTPSHTPTISAASPTSLDINAGSFINTPPSSSRVHRRSNSVDFTKKAGSFISMNSNSTPPSSPISSARVTSTSGIPRKRSIIIDLDKVTEEYKNCERRLSVASAKIQNKPVRNDQASGDTDGEVDEDGEFYMRELELSCQLPLSSELLLQSYCKNEDGQHHSDDEELDGRHKPKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVB9</accession>
    <name>G3AVB9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63749</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377903.1">
        <property type="nucleotide sequence ID" value="XM_007377841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVB9"/>
    <dbReference type="GeneID" id="18874894"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63749"/>
    <dbReference type="HOGENOM" id="CLU_971662_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVB9"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="287" mass="33162" checksum="387F7DB4B068DE34" modified="2011-11-16" version="1" fragment="single">MSSSEKAIWKMVESMVETVYHTANLFGITISAEVTLNIVETLNSLRIRTIHSLNDCVKDSILISNAKRQTNSTIFQFVDDSRFTYFLCRQRHKVANDTMLKVFNTVTPTPELFQYVETELVHAKSMAVAANCITDFAALSTNFNKVSKHTPEITAKFIKQVDSIYRSVIKFISLKSYENLERSAYRQITQDYYEICQTIDSGIVNGTKETIIQNSDLEPLLYRNENFRFHMENDSDIPDWVDEFYFRGNAEEAEEEEEESQGNTEIEESLPDIRMPSAHKPRFKKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVD0</accession>
    <name>G3AVD0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_63758</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007377853.1"/>
    </dbReference>
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    <dbReference type="STRING" id="619300.G3AVD0"/>
    <dbReference type="GeneID" id="18874898"/>
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    <dbReference type="eggNOG" id="ENOG502S6Q4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049642_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVD0"/>
    <dbReference type="OMA" id="IWINTND"/>
    <dbReference type="OrthoDB" id="62853at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
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        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="2">
        <location>
            <begin position="12"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="377" mass="42725" checksum="2A82D7B6A7C479CD" modified="2011-11-16" version="1">MSEDPAIDAFPPTTIVLAKVKGYPAWPAMVLDETILPEHILAKKPKTKSTATAAASASSSPKKKTNSSPTRILPVRFFSDDTYIWINSHDVKLLSRESIEEYFSSSSKKRRKDNLLETAYQLANDPPDMELFIKYGSRAAPPDEEEQVEEVVEKPPRKKSSAKPKAKAAPKAKVKPGPKPKETAKKQDPKKLAAEKKKQEEKKHMAEYDSDWGLDELNVYDPTTGDYIFDTEEKQESFFDQIPDATTIQNDLNKITNKFTKIEDKLVDLLLSEETIKEDAIIDQLSLLENMLKQLPKTVITRSKLLRVLILTKRKPEESFNYEQIKSKIDRVLKKLELEVRENTESELNPESKLGTPDIKDEPEVIEIKDNVANGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVG4</accession>
    <name>G3AVG4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">SPAPADRAFT_63793</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377949.1">
        <property type="nucleotide sequence ID" value="XM_007377887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVG4"/>
    <dbReference type="STRING" id="619300.G3AVG4"/>
    <dbReference type="GeneID" id="18874916"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63793"/>
    <dbReference type="eggNOG" id="ENOG502QVFG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_572623_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVG4"/>
    <dbReference type="OMA" id="ANIRWIA"/>
    <dbReference type="OrthoDB" id="2128042at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031606">
        <property type="entry name" value="Kch1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36424:SF1">
        <property type="entry name" value="LOW AFFINITY K(+) TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36424">
        <property type="entry name" value="PHEROMONE-REGULATED MEMBRANE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16944">
        <property type="entry name" value="KCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="401"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="408"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="477" mass="55607" checksum="38A563AC507408E0" modified="2011-11-16" version="1">MIAKRLYSIKSYNHFCLFNDIDEGSFFDWSCFLVYNELDNALQLLVADTPRQVINIMTLRFYATGGESNNNILANIRWIAETNLRLSIILGFMCLSLFIYAIFMIKFVFAMAMYIPIRCSLKGNEQHTIKSYCCNIVNKHVAKAVRQHHKPKHELLKKFSEDKLGDLPLFPRPDQTYNDIKKDPIIVRSETADSIPLTNLKPYTRKPPPIRNYSSSNSSYTMSKSSSGTSDAPTVLPFPLKKESLSNLREGNESYNSSNTLAQEMYREPEQMYSTLQQQQPQPSRIDLLRRSMSQHQYLPHSNSQDQIYDRDRSNQIYGQQHNNSYSSLTRTLPQSSSQQQLYNRSNSNHNLTYNNTSHQQLNYSNSPQEIYNRSNSNQHLMYSNDDRSQEIDQTYHNLTNQENPYESSSHSSSHNHSPVYDYEKQPDQYTNNNNDNREEEEEEEFNFPVRNFEQEKSSSESLPYPVRGVSTFFEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVI8</accession>
    <name>G3AVI8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bola-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_63561</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the BolA/IbaG family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007377641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVI8"/>
    <dbReference type="FunCoup" id="G3AVI8">
        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVI8"/>
    <dbReference type="GeneID" id="18874806"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63561"/>
    <dbReference type="eggNOG" id="KOG3348">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109462_0_1_1"/>
    <dbReference type="InParanoid" id="G3AVI8"/>
    <dbReference type="OMA" id="EIQNMHG"/>
    <dbReference type="OrthoDB" id="203381at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052275">
        <property type="entry name" value="Mt_Fe-S_assembly_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46188">
        <property type="entry name" value="BOLA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46188:SF1">
        <property type="entry name" value="BOLA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW29937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="12864" checksum="8556677C5CD9DDF2" modified="2011-11-16" version="1">MLRSFIPKAQLVTPRLSLASRSSVLQTIRHYSTPPDSMDEYESKIYGILEKEFTPSNLEVKDVSGGCGSMFAILIESQKFKGIPMIKQHRLVNETLKDEIKNWHGLQLKTKAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVL6</accession>
    <name>G3AVL6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_158219</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377731.1">
        <property type="nucleotide sequence ID" value="XM_007377669.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AVL6">
        <property type="interactions" value="580"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVL6"/>
    <dbReference type="GeneID" id="18871119"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_158219"/>
    <dbReference type="eggNOG" id="KOG1450">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002085_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVL6"/>
    <dbReference type="OMA" id="ELYPREW"/>
    <dbReference type="OrthoDB" id="79452at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037769">
        <property type="entry name" value="Abr/Bcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23182">
        <property type="entry name" value="BREAKPOINT CLUSTER REGION PROTEIN BCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23182:SF1">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN AT 1A, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GEF" evidence="4">
        <location>
            <begin position="284"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="5">
        <location>
            <begin position="667"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="1263"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="6">
        <location>
            <begin position="1319"/>
            <end position="1493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1184"/>
            <end position="1204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW29965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1495" mass="173051" checksum="D7E2E21B84D56238" modified="2011-11-16" version="1">MTEHKHPSPERRPIATRSFSSLVKSDYDYTTIKVAWMNVVISNNSNSNNSSSAVNNDPGLFRLYRAELKGSNLYLFKPTQNIRSFKLEEQEEGAELAPQLSHDENKLNSIHPNLQYDETTRTFTSYRLSLEPITPPGTNSIEAILHYILIYSNKEDNVDMFIELLPLLPDFGSILTLLANMLDAVYANKYGEVDLHQVVSRVLSILNNIDKNFPGYLLKSDIAPYILRIIEILTSKLKDAELKEINQFKTKMLHQQHKLIDLVGNNTTGNPLMQLNANSFLTLNIMELSRFISEIDLKFYSSWNPTIDKSLLVKQQLDTFYKKNPLVFNDTHIHYLSRLLINHILVEIHDYEFKARVLEKWIDLACLLDKLGDMNSWLGISSVIVSQPVLRLTKLWNHVSLDYIKLVRNDWSPVLFELDKNSFNDSEELHVIASRGLGRIYPKEKIIPYFGDLTIEVNNTVDIKELNIQMNNINYSLNRWHEYLHHIENSKDVLEYNNQVLLRYDNMGFIFSNESLNQVLYLGGDEPVKPIETTVTTRKPLHPELKLMLLKLVDINSDSMNMEGVMKLSLKLEPDLTECYLQESPKNHSTTSLNSIDSSDDPSMKLPNFNNSYFKINTNETNDMIISESSELQIDDELILKVTQNTTADGLGIDVEDILNGVTTSSDKFIPKYGTLDRLLDLLVCDSSWFIDSVKLDLQEYRSVFLLNYYAFISTHDLFEKLTYRFKHAENAVSSLRGQFPNWEAPVNENLTIKDYSKIMSIQINIIKALIVIINNFYHPNFTTDLSNRTVFTSLLKLINSTIVSYYTNTIHTEQFDNLIMYYKKLKQLYVKKSYRPIEQSKFDVYLNHEFKFNNSLSDVPINRNLPGHSNIHKIEKFLVKFNKFLFVFYKGITIEDWMKVYRILETMYYNNDLLNVDLQRNSTADEQLIISTVFTYLESLSDDTGLVIDRFPLVFQKLFKLYCKFKTYLLIQLSNITVEERLDRMKALLIMAKIANMKRKQFEFEGTCSTIPSFIETAITNVIQSRVSRSYSSLWIEASRQLNPAHAANYDDITSLFPNLDKSELNNDECLLPCFGWIIENLIEINRVPSYKLNMINFNKRHLVYKLIKELSIEDSDITKHDTREFDFLLRLDERVKFKVSMDNSNRLFRSLIREQKRILMTDNQKKIIKKSINSPTVMHSVAKKSSTNSLKRQSLKPNGTSSSNKFKLTGLFNRARPLLSSEQKILAVKDLPPPSIDNCQYKKPVVVPLRNRKIFPIYHMRHCFKLDSDVTNDSYLLQAVTEDDVNDWLIKLDYANRHWFMSRIMNSTVAKSVTFGIPLGYHGDCPKVLTILFDIIELGLDEVGIYRISCSTSELLNMKAYIDRYGTVERTIDTHTATSLVKAYFRELPDSIFTDEVINLLFEIRQNLTSEKLIEVLAKLPQANYTTLKTLTKHLSKVCENHEINKMTSSNLATVIAPALTESSHLQCLVNNFGFMNKIMDNLIEMYDDVFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVN4</accession>
    <name>G3AVN4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_158772</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377965.1">
        <property type="nucleotide sequence ID" value="XM_007377903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVN4"/>
    <dbReference type="STRING" id="619300.G3AVN4"/>
    <dbReference type="GeneID" id="18871133"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_158772"/>
    <dbReference type="eggNOG" id="ENOG502QSNK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_522322_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVN4"/>
    <dbReference type="OrthoDB" id="4066896at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057454">
        <property type="entry name" value="Bud3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021895">
        <property type="entry name" value="Bud3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12015">
        <property type="entry name" value="Bud3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25351">
        <property type="entry name" value="PH_BUD3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="2">
        <location>
            <begin position="157"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="300"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="522" mass="59188" checksum="422135595EB8502A" modified="2011-11-16" version="1" fragment="single">MILDSSSRFYTSVENLHDKYQSSMTRRALAVTNLKNCNRLINHGGELPELPLWDGINAGQLANSMTKLTTPEIGNKLLELGLVQPHALNSFVVDVVYDNSSGSKNIIEDNNKLVNLLGIQSQHLFDPLLEYSPESINITYSPPMDNTVQDSELVSAIINELLTVQENYTLGLVELLQNFLIPLRITVLNSKNPTTTITKLNTIFPPTIDEITRINCILHAALQNASQYNHQEVIKAMAMFMPYFYKAYIRHEANVNGFNKNLHAFAQKNRSNILDNLNINKSAYSTREIESVVIGALLELPKLKLIIQRLKSEIKDCSEETEQYYNTIVKVIDAFGTDAITIDISKRIFTPTGKILTELATNWPKELQYGWLTRKVVGIYELKNIKSDSKYDRDVIIIFSDNVLILNIVDDDYYTNDGLSVSDILMHSLINQKPLPKYDTFPKMEVTAWSKITDIIVTTYQGTNGEYIRILNTSLNGFQTTNSNYSRNYQIKTPNITGDDIIQLITKSKILSKSQPFHLFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVV7</accession>
    <name>G3AVV7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">DNA polymerase</fullName>
            <ecNumber evidence="20">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">SPAPADRAFT_52847</name>
    </gene>
    <organism evidence="26">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19 20">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 20">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="20"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377768.1">
        <property type="nucleotide sequence ID" value="XM_007377706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVV7"/>
    <dbReference type="FunCoup" id="G3AVV7">
        <property type="interactions" value="777"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVV7"/>
    <dbReference type="GeneID" id="18871725"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52847"/>
    <dbReference type="eggNOG" id="KOG0969">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000203_2_0_1"/>
    <dbReference type="InParanoid" id="G3AVV7"/>
    <dbReference type="OMA" id="CNNCRPR"/>
    <dbReference type="OrthoDB" id="2414538at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140445">
        <property type="term" value="C:chromosome, telomeric repeat region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
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        <property type="project" value="EnsemblFungi"/>
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    <dbReference type="GO" id="GO:1904161">
        <property type="term" value="P:DNA synthesis involved in UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903459">
        <property type="term" value="P:mitotic DNA replication lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006278">
        <property type="term" value="P:RNA-templated DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05777">
        <property type="entry name" value="DNA_polB_delta_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05533">
        <property type="entry name" value="POLBc_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.730:FF:000004">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000351">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.730">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025687">
        <property type="entry name" value="Znf-C4pol"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00592">
        <property type="entry name" value="pol2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF23">
        <property type="entry name" value="DNA POLYMERASE DELTA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-C4pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 20" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 20" id="KW-0235">DNA replication</keyword>
    <keyword evidence="17 20" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14 20" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15 20" id="KW-0408">Iron</keyword>
    <keyword evidence="16 20" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6 20" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18 20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 20" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="23">
        <location>
            <begin position="220"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="22">
        <location>
            <begin position="516"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="domain" description="C4-type zinc-finger of DNA polymerase delta" evidence="24">
        <location>
            <begin position="983"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF14260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="EGW30002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1079" mass="123888" checksum="CD357E843009195F" modified="2011-11-16" version="1">MPGEKRAFEEVHQDQAHSLPVSSQIISSQGGLTASEEQVLRQVKRPNGNQEPSEFERELLDMTQAVRGMEQEEDQNWSRPELSADFNSELEDVSFQQLDAEEFNERDNTFARFFGITEAGHSVLCNVVGFIHYFYVPVPKGFVKDQHLQQFTNYLHANYEGIDKVEITLKETIWGYNRNIKTPFFKVFVNNTRNITKLRTAFERGDIRFENLFSETVSYDNINYLMRLMIDCKITGMSWITLPKSKYKMVPDNLKISSCQIECSIDYRDLITHPSEGEWLKMAPLRILSFDIECAGRKGIFPEAQHDPVIQIANVVSKSGESKPFVRNVFTVNTCSPIIGSQIFEHAKEEEMLMHWRQFITEVDPDVIIGYNTSNFDIPYLLDRAKALGLKDFPFFGRLKRIKQEVKDAVFSSRAYGTRENKVVNIDGRMQLDLLQFVQREYKLRSYTLNSVSAHFLGEQKEDVQHSIITDLQNGTKETRRRLAVYCLKDAFLPLRLLDKLMCLVNYTEMARVTGVPFSYLLSRGQQIKVISQLFRKCLEEDIIIPNMKSEGTNEEYEGATVIEPIRGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLSKSAIQAYGLTEEDYTRTPNGDFFVKSHKRQGILPTILNELLTARKKAKADLKKETDPFKKDVLNGRQLALKISANSVYGFTGATIGKLPCLAISSSVTAFGREMIEKTKNEVQEYYSKKNGHPFDAQVIYGDTDSVMVKFGYQDLETCMKLGEEAANYVSTKFKSPIKLEFEKVYYPYLLINKKRYAGLYWTRPDKFDKMDTKGIETVRRDNCRLVQNVITKVLQFILEERDVEKAQRFVKQTIADLLQNRVDLSQLVITKAYAKHDYSAKQAHVELAERMKKRDPGSAPTLGDRVAYVIVNTGSDKNYEKSEDPLYVLENSLPIDVKYYLDQQLTKPLERIFNPILGDGKTKELLAGAHTRTIKMSAPKTGGLMRFAKKSEVCINCKTPLKSENHGALCPNCIREGKGPELYSNALSQMNYLENKFSRLWTECQRCQGSLHQEVLCGNKDCPIFYMRTKAQKDVHQQAMELVKWENTVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADY5</accession>
    <name>G3ADY5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63942</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372121.1">
        <property type="nucleotide sequence ID" value="XM_007372059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ADY5"/>
    <dbReference type="FunCoup" id="G3ADY5">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="GeneID" id="18874951"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63942"/>
    <dbReference type="eggNOG" id="ENOG502S3TQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097985_0_0_1"/>
    <dbReference type="InParanoid" id="G3ADY5"/>
    <dbReference type="OrthoDB" id="3981113at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="227" mass="25348" checksum="85A81E61D78F1F7B" modified="2011-11-16" version="1">MSHPDASVKSICSGKINLDSPDDIYFNNNNSYNYPAEDLDIDDEAIDETFKNSGYEYLDDISSKESNYSVTSKNLKALDNQRVKFNDTNGTNLSLVSTESDYQRILEDSTSLLSPDFVGDSLSRPISRSSTTSCLSTTATKDGIEGRRFHRYGPTAYSSNIIANMVHSQQLQGKIAIPEPRNSFESNTSVGEDSTDDPNNLKKKRPISKHRHSQLNREQESIESDKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE07</accession>
    <name>G3AE07_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N(6)-L-threonylcarbamoyladenine synthase</fullName>
            <ecNumber evidence="1">2.3.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">N6-L-threonylcarbamoyladenine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_132219</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-threonylcarbamoyladenylate + adenosine(37) in tRNA = N(6)-L-threonylcarbamoyladenosine(37) in tRNA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="1"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372953.1">
        <property type="nucleotide sequence ID" value="XM_007372891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE07"/>
    <dbReference type="FunCoup" id="G3AE07">
        <property type="interactions" value="597"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AE07"/>
    <dbReference type="GeneID" id="18869645"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132219"/>
    <dbReference type="eggNOG" id="KOG2708">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023208_2_2_1"/>
    <dbReference type="InParanoid" id="G3AE07"/>
    <dbReference type="OMA" id="HHRSWVV"/>
    <dbReference type="OrthoDB" id="10254073at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008252">
        <property type="term" value="F:nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:tRNA N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd24132">
        <property type="entry name" value="ASKHA_NBD_OSGEP_like_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000038">
        <property type="entry name" value="Probable tRNA N6-adenosine threonylcarbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000295">
        <property type="entry name" value="Probable tRNA N6-adenosine threonylcarbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01446">
        <property type="entry name" value="Kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034680">
        <property type="entry name" value="Kae1_archaea_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017860">
        <property type="entry name" value="Peptidase_M22_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03722">
        <property type="entry name" value="arch_KAE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF14">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01016">
        <property type="entry name" value="GLYCOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Gcp-like" evidence="11">
        <location>
            <begin position="48"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="154"/>
            <end position="158"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW35541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="372" mass="41310" checksum="0D82E7BBFFFFAC02" modified="2011-11-16" version="1">MTVDLDKYLKPGKDHYIALGLEGSANKLGVGVIRHNQGQLTSSNRAEVLSNIRDTYIAPPGEGFLPRDTARHHRNWVVRVIKRALAVAKIKGTDIDVICFTQGPGMGSPLQSVVIAARTLAQLWKIPLMGVNHCVGHIEMGREITGADNPVVLYVSGGNTQVIAYSKQRYRIFGETLDIAIGNCLDRFARTLKIPNEPAPGYNIEQMAKKGKHLVNLPYTVKGMDLSMSGILANIDSIAKDMFGKQNKQLIDEETGEPITAEDLCFSLQEILFSMLVEITERALAHVNSNQVLIVGGVGSNQRLQEMMKLMIEDRKNGQIYATDERFCIDNGIMIAHAGLLSYRMGQVTDLDHTVCTQRFRTDEVFVEWRDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE33</accession>
    <name>G3AE33_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA (guanine-N(7)-)-methyltransferase</fullName>
            <ecNumber evidence="11">2.1.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Transfer RNA methyltransferase 8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA (guanine(46)-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA(m7G46)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TRM8</name>
        <name evidence="13" type="ORF">SPAPADRAFT_48549</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42708"/>
            <dbReference type="Rhea" id="RHEA-COMP:10188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10189"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10 11">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Forms a complex with TRM82.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.33" evidence="11"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372980.1">
        <property type="nucleotide sequence ID" value="XM_007372918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE33"/>
    <dbReference type="FunCoup" id="G3AE33">
        <property type="interactions" value="548"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AE33"/>
    <dbReference type="GeneID" id="18871238"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48549"/>
    <dbReference type="eggNOG" id="KOG3115">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050910_3_1_1"/>
    <dbReference type="InParanoid" id="G3AE33"/>
    <dbReference type="OMA" id="LPNYFAK"/>
    <dbReference type="OrthoDB" id="47276at2759"/>
    <dbReference type="UniPathway" id="UPA00989"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106143">
        <property type="term" value="C:tRNA (m7G46) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008176">
        <property type="term" value="F:tRNA (guanine(46)-N7)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000060">
        <property type="entry name" value="tRNA (guanine-N(7)-)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03055">
        <property type="entry name" value="tRNA_methyltr_TrmB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025763">
        <property type="entry name" value="Trm8_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00091">
        <property type="entry name" value="tRNA (guanosine(46)-N7)-methyltransferase TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417">
        <property type="entry name" value="3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417:SF16">
        <property type="entry name" value="TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 11" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="10"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="136"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="172"/>
            <end position="173"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="270"/>
            <end position="272"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW35568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="298" mass="35182" checksum="ECFD0B24821E9F83" modified="2011-11-16" version="1">MPKPPQPPCTKDKETQLREQQEAEIKRRAYRENKQNARKKVKHARFEEPTDVSPVDLPRKRFYRQRAHSNPFSDHRLDYPNSPDTMDWSKMYPNFYDSELGKMTSKVEIADIGCGYGGLLTQLGPSFPESLVLGMEIRVQVTQYVEDRIIALRNKHAQDDYKYDNISVIRGNAMKFLPNFFYKSQLSKMFFCFPDPHFKQRKHKARIITNTLLSEYAYVLKEGGVIYTITDVEDLHNWMVQHLDEHPLFERLSKEWEDQDKCVEIMYNSTEEGQKVTRNKGSKWVACYRRLPNPEECV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE59</accession>
    <name>G3AE59_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sterol-4-alpha-carboxylate 3-dehydrogenase ERG26, decarboxylating</fullName>
            <ecNumber evidence="13">1.1.1.170</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18 19">C-3 Sterol dehydrogenase ERG26</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16 17">C-4 decarboxylase ERG26</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Sterol-4-alpha-carboxylate 3-dehydrogenase erg26, decarboxylating</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">SPAPADRAFT_58811</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>4beta-methylzymosterol-4alpha-carboxylate + NADP(+) = 3-dehydro-4-methylzymosterol + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:33447"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:50593"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:64925"/>
            <dbReference type="EC" id="1.1.1.170"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:33448"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="12">Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 4/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterotetramer of ERG25, ERG26, ERG27 and ERG28. ERG28 acts as a scaffold to tether ERG27 and other 4,4-demethylation-related enzymes, forming a demethylation enzyme complex, in the endoplasmic reticulum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 3-beta-HSD family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.170" evidence="13"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373005.1">
        <property type="nucleotide sequence ID" value="XM_007372943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE59"/>
    <dbReference type="FunCoup" id="G3AE59">
        <property type="interactions" value="416"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AE59"/>
    <dbReference type="GeneID" id="18872621"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58811"/>
    <dbReference type="eggNOG" id="KOG1430">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_6_8_1"/>
    <dbReference type="InParanoid" id="G3AE59"/>
    <dbReference type="OMA" id="YNITNHE"/>
    <dbReference type="OrthoDB" id="10058185at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102175">
        <property type="term" value="F:3-beta-hydroxysteroid dehydrogenase (NAD+)/C4-decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09813">
        <property type="entry name" value="3b-HSD-NSDHL-like_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000346">
        <property type="entry name" value="C-3 sterol dehydrogenase/C-4 decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002225">
        <property type="entry name" value="3Beta_OHSteriod_DH/Estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01073">
        <property type="entry name" value="3Beta_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0752">Steroid biosynthesis</keyword>
    <feature type="domain" description="3-beta hydroxysteroid dehydrogenase/isomerase" evidence="20">
        <location>
            <begin position="11"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00067470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00081267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00081452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00082106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EGW35593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="351" mass="38835" checksum="E85538B3FB0324C9" modified="2011-11-16" version="1">MTKSPLNSVLLVGGSGFLGLHLIEQFHRHCPETKITIFDIRPLPEKLSQYFTFNPSQIHFIKGDLTAESDVSNAITSSECDVIVHAASPMHGLPQEIYEKVNVEGTKNLLAQAKKLGVKAFVYTSSAGVIFNGQDVYNGDESWPYPDVHMDGYNETKAIAETAVMNANDPAKGFLTVCLRPAGIFGPGDRQLVPGLRASAKLGQSKYQLGDNNNLFDWTYVGNVADAHVLAATKILDPEFSQQLGGETFFITNDAPTYFWTLARTVWKADGYVDKYYIKLSRPVAIVLGYFSEFFSKLAGKEPGITPFRVKVVCATRYHNISKAKTILGYKPAVDLETGIKYTLDWMNEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE68</accession>
    <name>G3AE68_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_58821</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373014.1">
        <property type="nucleotide sequence ID" value="XM_007372952.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE68"/>
    <dbReference type="STRING" id="619300.G3AE68"/>
    <dbReference type="GeneID" id="18872626"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58821"/>
    <dbReference type="eggNOG" id="ENOG502QQ16">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013298_0_0_1"/>
    <dbReference type="InParanoid" id="G3AE68"/>
    <dbReference type="OMA" id="GFHGMAM"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646:SF1">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="380" mass="43980" checksum="0B15241E818ED5B8" modified="2011-11-16" version="1">MLPIEVVIPTFKDYEREAEFCKVFLPKHNAKCIIVEEAFGKDIVTQIGSYQFKAAGLAISSFQHTLLLDSDNNPVMAPDRLFEAKVYKTNGMVLWPDYWPRTMSPEWHDIIGKPYSLTEKVRDGRFPLVNVQHLTPEQEEKTKFTELKGTLSDLSTESGQVMINKGTHGKVVLMILYYNMFGPELYYKLFSLGALGEGDKDTFATAAFACGKQYYQVKSFIKTYGYHDDGYNGMTMAQKDPEEDYQLFMRLEKEYRDKGIDWDKDAKEAFGGDNDVPVFTLHCNIKKINPFEYIKDDKISDLSANRLKHRFYSKFKMRLPEDGIDGVSTVKEIDFELSRWESVEELLCEKKIKFTITKEKDIESVCEYIKNTIAWLKVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEH8</accession>
    <name>G3AEH8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63972</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372152.1">
        <property type="nucleotide sequence ID" value="XM_007372090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEH8"/>
    <dbReference type="GeneID" id="18874954"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63972"/>
    <dbReference type="eggNOG" id="ENOG502QQV3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_738031_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEH8"/>
    <dbReference type="OMA" id="IDNGRIC"/>
    <dbReference type="OrthoDB" id="4095993at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="375" mass="42801" checksum="A5CC03C4898B078F" modified="2011-11-16" version="1">MNDTYDEFTNLGDIPKQDGTEVYENSSSSQPHQHQRQHQQMVPHDLSEEFGLSKDSPDVSMLPPSVVSMSKEEIVIDNGRICPYCHKEFTSKGSLGRHLDSKKGDPVHPLDEITAIRSLTKRRIVEVPRGSRKAAQPAPPSEGTSKKRKVIKKSMARSQISVDPASGQREKSKLRRKLRDRRIKAKLLTNEWLLDQFGNYPLVEPKPTDPGSKFAHITALYLPVSKWPDSYPDNSSYDLVVNELKTRDRLDLLSILTIAFHTFEQLPLQHKVDIWRRETLRCLRPTIGSFSIADLHELKAVTSKKEQGHFEEICSRDNLSSYVDLDDGREPLPVEEEEEAEEEGIDQVKDEPEIFMAHLTDTPGSMIDEFTMFRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEL2</accession>
    <name>G3AEL2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutathione hydrolase</fullName>
            <ecNumber evidence="8">2.3.2.2</ecNumber>
            <ecNumber evidence="8">3.4.19.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Gamma-glutamyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Gamma-glutamyltranspeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_57841</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Cleaves the gamma-glutamyl peptide bond of glutathione and glutathione conjugates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 8">
            <text>an N-terminal (5-L-glutamyl)-[peptide] + an alpha-amino acid = 5-L-glutamyl amino acid + an N-terminal L-alpha-aminoacyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:23904"/>
            <dbReference type="Rhea" id="RHEA-COMP:9780"/>
            <dbReference type="Rhea" id="RHEA-COMP:9795"/>
            <dbReference type="ChEBI" id="CHEBI:77644"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:78599"/>
            <dbReference type="ChEBI" id="CHEBI:78608"/>
            <dbReference type="EC" id="2.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>an S-substituted glutathione + H2O = an S-substituted L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:59468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="ChEBI" id="CHEBI:143103"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 8">
            <text>glutathione + H2O = L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:28807"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:61694"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Sulfur metabolism; glutathione metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the gamma-glutamyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.2" evidence="8"/>
    <dbReference type="EC" id="3.4.19.13" evidence="8"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372186.1">
        <property type="nucleotide sequence ID" value="XM_007372124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEL2"/>
    <dbReference type="FunCoup" id="G3AEL2">
        <property type="interactions" value="166"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEL2"/>
    <dbReference type="MEROPS" id="T03.012"/>
    <dbReference type="GeneID" id="18872217"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57841"/>
    <dbReference type="eggNOG" id="KOG2410">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014813_4_1_1"/>
    <dbReference type="InParanoid" id="G3AEL2"/>
    <dbReference type="OMA" id="ICGMGPP"/>
    <dbReference type="OrthoDB" id="1081007at2759"/>
    <dbReference type="UniPathway" id="UPA00204"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103068">
        <property type="term" value="F:leukotriene C4 gamma-glutamyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.40:FF:000001">
        <property type="entry name" value="Gamma-glutamyltranspeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00066">
        <property type="entry name" value="g_glut_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686">
        <property type="entry name" value="GAMMA GLUTAMYL TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686:SF9">
        <property type="entry name" value="RE13973P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="422"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="440"/>
            <end position="442"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="492"/>
            <end position="493"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="515"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="615" mass="68359" checksum="CDDA686629AFD140" modified="2011-11-16" version="1">MKTSTFEPKSTKTIRYYYLFTTITLVLVIHKFLSFGNTSPPPISFQYVTNEDPIQHIGQPTLNPDSKHRHTAGNAMVACDVPLCSTMGKQILQSGGNAADAAVTVALCIGSINSHSSGIGGGALILSKHGRDVVSIDAREMAPSGAFKDMYEHDGSLAGELLSKIGGLSIGIPGELAGLYQLFKLHGSGHLTWAELIDPVIKLNRDGFECSELFEGVIEKQSKILDLIPSIKTNWDFIFNKGEHLRHGDWIKRKNYANTLELIAKNGSSSIFYDPQGPIVPNLVKTIHQYGGIVTSEDFTKYEVNIDEAITSTFQNKTIYTASGISSGLALVSGLKFFDRVYNKDDEPTSFNHKLIESFKWLTSVRSRLGDVSPEYQQELISNYTSDHWIDEVLSTGKFSPNTTFPWKNYDPKYDIVEDHGTAHFSIVDKDDNAVAFTTTVNLLFGSMIYDPATGIILNDEMDDFSRPQISNAFNLTPSIYNFIKPYKRPLSSMAPTIIMDSHNRTELVIGAAGGSRITSAILQAIVRQFYRGTSLLQAIGFPRMHHQLIPQYVMIENLTMFDEEFVGVREKLEAMGHEFVETGTLTAMNGIRRVGQKLEGVCDWWRKRGEADGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEP8</accession>
    <name>G3AEP8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">pH-response transcription factor pacC/RIM101</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_53851</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the pacC/RIM101 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373086.1">
        <property type="nucleotide sequence ID" value="XM_007373024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEP8"/>
    <dbReference type="STRING" id="619300.G3AEP8"/>
    <dbReference type="GeneID" id="18871815"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53851"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014727_1_0_1"/>
    <dbReference type="InParanoid" id="G3AEP8"/>
    <dbReference type="OMA" id="KDHTHIH"/>
    <dbReference type="OrthoDB" id="3437960at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000125">
        <property type="entry name" value="Putative zinc finger protein 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000839">
        <property type="entry name" value="Zinc finger protein 691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050329">
        <property type="entry name" value="GLI_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818:SF139">
        <property type="entry name" value="PAIR-RULE PROTEIN ODD-PAIRED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818">
        <property type="entry name" value="ZINC FINGER PROTEIN ZIC AND GLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="394"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="423"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="451"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="479"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="507"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="530" mass="60280" checksum="820F0B78F9B313A8" modified="2011-11-16" version="1">MDDLEFCKDVFCDDLDDCFFTNCDNAGDCCCMGDPACNGDCWALSNKNIECCNDPNCVDYTTKQSPKPQIQQLSELAHHCNDEACDYNSPPPSSHQQDHLCQVQSSKRSLFEDLITNVHANLNNTSQMKHNDFEIHFPHECHAEESKPTNHHHFHQSCFHTTIPEASSSAGARASCYDEKLMSNFDYDFIVEFNNFNNSLANADPVATSSASPDQLVYPCQWEKCFKRVTDDTILNHVMEEHIRNEYPDTISDTAYQCEWQDCNFINSDFKLLVDHLKNHKQQTKKQFILPNQSILTPSSTTKSYESSPIADYPTVKDEHPNVNITSIKILPKKPKASPTPPDPTHTCKWEIGHDSNGNAIPCNKTHISEADLQDHLLNDHIGCGKSKYFCNWIGCERHMGKQFTQRQKLLRHIHIHTGYKPCQCHICGASFAVEGMLTQHLRTHSGEKPFPCSICGKRFATRSSLSIHHRVHTGEKPLVCKWPGCGKRFSESSNLTKHMKIHSKLFECEVCGSVFEKKVDYTKHVKTHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFB1</accession>
    <name>G3AFB1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Transcription elongation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_131482</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the TFS-II family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372312.1">
        <property type="nucleotide sequence ID" value="XM_007372250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFB1"/>
    <dbReference type="FunCoup" id="G3AFB1">
        <property type="interactions" value="962"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFB1"/>
    <dbReference type="GeneID" id="18869580"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131482"/>
    <dbReference type="eggNOG" id="KOG1105">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037637_2_0_1"/>
    <dbReference type="InParanoid" id="G3AFB1"/>
    <dbReference type="OMA" id="RFVVMTH"/>
    <dbReference type="OrthoDB" id="44867at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001139">
        <property type="term" value="F:RNA polymerase II complex recruiting activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001193">
        <property type="term" value="P:maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045899">
        <property type="term" value="P:positive regulation of RNA polymerase II transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031440">
        <property type="term" value="P:regulation of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00183">
        <property type="entry name" value="TFIIS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13749">
        <property type="entry name" value="Zn-ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000001">
        <property type="entry name" value="Probable Transcription elongation factor S-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.30:FF:000003">
        <property type="entry name" value="Transcription elongation factor S-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.10:FF:000007">
        <property type="entry name" value="Transcription elongation factor S-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.30">
        <property type="entry name" value="Transcription elongation factor S-II, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035100">
        <property type="entry name" value="TF_IIS-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003617">
        <property type="entry name" value="TFIIS/CRSP70_N_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036575">
        <property type="entry name" value="TFIIS_cen_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006289">
        <property type="entry name" value="TFSII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01385">
        <property type="entry name" value="TFSII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477:SF0">
        <property type="entry name" value="IP08861P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477">
        <property type="entry name" value="TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07500">
        <property type="entry name" value="TFIIS_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006704">
        <property type="entry name" value="TF_IIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00510">
        <property type="entry name" value="TFS2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00509">
        <property type="entry name" value="TFS2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46942">
        <property type="entry name" value="Elongation factor TFIIS domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="16">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS central" evidence="17">
        <location>
            <begin position="136"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS-type" evidence="15">
        <location>
            <begin position="254"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="78"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="88"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW34900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="296" mass="33191" checksum="26D8E3D27091A567" modified="2011-11-16" version="1">MDTKEIKSTISNLEKSTDDTTILKLLNILNDGVKPSEKVLRETKVGVAVNKFRSHENSEISNLVKKMIRNWRDAVQHEKISKKKVSGSSTPSTPATPATPSSSTNLNSIDGAKKIPTGPRNPKTDGVSTELYDNPTRNASVSALYTSLAVERGDPSELILKIASEIESEVFKSEYSKVTDSYRNKLRSFTMNLRNKKNPELRERLLTQAILAANFIKMTPSEMAPESLKKEIEKLNKQNLFDAQGATEKRAVTDRFTCGKCKHKKVSYYQMQTRSADEPLTTFCTCENCGNRWKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFK4</accession>
    <name>G3AFK4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HORMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_53356</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372406.1">
        <property type="nucleotide sequence ID" value="XM_007372344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFK4"/>
    <dbReference type="FunCoup" id="G3AFK4">
        <property type="interactions" value="1130"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFK4"/>
    <dbReference type="GeneID" id="18871776"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53356"/>
    <dbReference type="eggNOG" id="KOG3285">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072097_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFK4"/>
    <dbReference type="OMA" id="WQFDVEI"/>
    <dbReference type="OrthoDB" id="4951845at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990333">
        <property type="term" value="C:mitotic checkpoint complex, CDC20-MAD2 subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044774">
        <property type="term" value="P:mitotic DNA integrity checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902499">
        <property type="term" value="P:positive regulation of protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003511">
        <property type="entry name" value="HORMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045091">
        <property type="entry name" value="Mad2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842:SF11">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02301">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56019">
        <property type="entry name" value="The spindle assembly checkpoint protein mad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50815">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HORMA" evidence="7">
        <location>
            <begin position="7"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="199" mass="22940" checksum="F57B665CFD0921C5" modified="2011-11-16" version="1">MSKLALKGSSKIVSDYFEFSINTILFQRGIYPAEDFVTVRKYDLLMLVTNDDDVKAYIDTIMKQIKKWIYGKRLLRFVLAIISKSTYETIERWEFNLDIISQDADNSSDKPQEEIQKEIRAIIRQITSSVSYLPVLKEDDDYTFNVLVYTDPTTTVPIEWCDTQGDGKELEGDNVESVKFASFNTNLHEIGTAVSYKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG06</accession>
    <name>G3AG06_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58332</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372557.1">
        <property type="nucleotide sequence ID" value="XM_007372495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG06"/>
    <dbReference type="GeneID" id="18872408"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58332"/>
    <dbReference type="HOGENOM" id="CLU_2559758_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG06"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="82" mass="9300" checksum="15F76D727300C7E6" modified="2011-11-16" version="1">MPGVPNWGNLLGFENQTSPPLFIDKRFSCAWLQLNFGWGLRLIVASLPSFFSRIILSHYCPLVHAIGHILEEDYQKATADHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG71</accession>
    <name>G3AG71_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_133048</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372622.1">
        <property type="nucleotide sequence ID" value="XM_007372560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG71"/>
    <dbReference type="STRING" id="619300.G3AG71"/>
    <dbReference type="GeneID" id="18869714"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133048"/>
    <dbReference type="eggNOG" id="KOG2513">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013423_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG71"/>
    <dbReference type="OMA" id="MFGPVWP"/>
    <dbReference type="OrthoDB" id="296386at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049456">
        <property type="entry name" value="Anoctamin_N_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF73">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20877">
        <property type="entry name" value="Anoctamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="271"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="426"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="502"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="557"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="601"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin alpha-beta plait" evidence="8">
        <location>
            <begin position="9"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="7">
        <location>
            <begin position="157"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="792"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="798"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="808"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="831"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="846" mass="96250" checksum="B016C6A132D8C0F0" modified="2011-11-16" version="1">MTLAIQELNPDFYLSIDYPQENGIPTSAAKAQLTKLVETLYADGFAAQIRPGDASHLLVFIKLLSSKCIDEIEKDLVKSFEFGVTAKDDVYSARLRVINKYLRTRKSIGGCGITPNAGEWKFVNDIVPITSAFDDSTLIEDLKSSFTESPVLTTNRIKNQYGIKIALYFEYFKFYMLWLVSLSVIGLVSYFKVKKTYSLAYTLVNLLWGTLFITFWNRKQSFLVNFWGVQNCHLVEEHYSELAELNSRHEPKNDPAPQESRRYVLFLKQLAFVPIALGFTAVLVSYQLSCFVIEIFLTDVYDGPGKSLLTLLPTILISVFVPILTIVYNLVIAQVIKWENHDNSYSRNNSVLIKTFVLNFLTSYVPLIITSFIYLPFAHLIQPHLGDIKSTISTYVTKDRFYYKYLIMLKKQEDFKMNQNRLNAQFFYFIVTNQVIQFILKYFLPLILAKLTDLVETKLLKKEKFEPKDEPTEAVWLHNIRSSLKRPVYDVNDDFRSLILQYGYLIMFGPVWPLAPLVCLIFNLIIFRVDQFKLANAKYFKPPLPSRSDSIHPWNKSLFFLTWVASVISPVVTAFYRHGTQPPKTLGQLALKNASVNVSSFLKLMLLMFTTEHGFLILSYTLYRLSDLFKSDIEWQNDFGSNDLKLRLEYYKDKEKPNIVMINDVGWEGFTVENTLGLAAPSDTDAAIEKIDTPKGGYSTSAQANITQAASRVEEKTKEIEQLPDTVPVPVPAVVDDKVEDISTEPIVIGSKEALDKIKDSTDEIISTKNSEGDLQYSTIDSNAHAEVTKVFPREDVSTSTSSNQAPSITSSSEVSRSDSMNSSSRDSRDKGKKRSGLKKIFKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGC8</accession>
    <name>G3AGC8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_58482</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 32 different proteins and 1 molecule of RNA (18S). The 60S subunit contains 45 different proteins and 3 molecules of RNA (25S, 5.8S and 5S).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372679.1">
        <property type="nucleotide sequence ID" value="XM_007372617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGC8"/>
    <dbReference type="FunCoup" id="G3AGC8">
        <property type="interactions" value="1198"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGC8"/>
    <dbReference type="GeneID" id="18872467"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58482"/>
    <dbReference type="eggNOG" id="KOG3166">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055193_0_1_1"/>
    <dbReference type="InParanoid" id="G3AGC8"/>
    <dbReference type="OMA" id="RMVKWPA"/>
    <dbReference type="OrthoDB" id="29563at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001921">
        <property type="entry name" value="Ribosomal_eL8_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00882">
        <property type="entry name" value="RIBOSOMALL7A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="8">
        <location>
            <begin position="125"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00063735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="262" mass="28454" checksum="A7D521800B8FBBD1" modified="2011-11-16" version="1">MTQPSSKKVAPAPLAAKSKVTASPKNPLIESTPKNFGIGQSIQPTRNLSRFVRWPEYVRLQRQKKILSLRLKVPPSIAQFSQTLDKNTAAQAFKLFNKYRPETSAEKKERLTKEAAAVAEGKSAKDASPKPVVVKYGLNHVVSLIENKKAKLVLIANDVDPIELVVFLPALCKKMGVPYAIVKGKARLGTLVHKKTSAVAALTEVNGADEAELSKLVSTINANFIEKYEDNRRHWGGGIMGSKANDKIAKRAKASASVATSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGD6</accession>
    <name>G3AGD6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_58489</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372687.1">
        <property type="nucleotide sequence ID" value="XM_007372625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGD6"/>
    <dbReference type="STRING" id="619300.G3AGD6"/>
    <dbReference type="GeneID" id="18872470"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58489"/>
    <dbReference type="eggNOG" id="KOG2391">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043965_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGD6"/>
    <dbReference type="OMA" id="RFYHPYL"/>
    <dbReference type="OrthoDB" id="306304at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072666">
        <property type="term" value="P:establishment of protein localization to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043162">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037202">
        <property type="entry name" value="ESCRT_assembly_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017916">
        <property type="entry name" value="SB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09454">
        <property type="entry name" value="Vps23_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140111">
        <property type="entry name" value="Endosomal sorting complex assembly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SB" evidence="6">
        <location>
            <begin position="345"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="24"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="41"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="111"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="165"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="424" mass="46754" checksum="AA671E28FFCD5144" modified="2011-11-16" version="1">MVDPPVSSSPPMVHASSSSDAVPQLPPKPAKVPLHQPPVPNTTQVQSPQLTGPPSVGIPQSANPQYTGPRNEVNTMGPQTTGPPLPAKPPKLQSPTTQTINPIPLKYQAPLPLPGEQPQSQPQPPVQPEYQHFNQASPPPPFSATHFNYPKETSASPIQPIPYGPQKAAPSPQQLPPQQHNIQPGNRYTQQRVEHPQERHPVHVQHQPNLPIAKQPVVDLMDAVASSSKGSTSTQSDAQRRQMLEQLSIKINSYLTDPTNGSVNSDVPMVNEHANKAEALYNQLNHHYQQALGNTRNLDDHLAYLTTQLTHVSNLNQELMKLEELNSASKDKVYTGTNTALTLDDLIIPDSPLVKQLYETVSEIKSIKDTINLISGNFHNQPELINDSRLDVCIKTVRNLGRELFWLELTKDEIAHSIMNLPRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGF4</accession>
    <name>G3AGF4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_58513</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372705.1">
        <property type="nucleotide sequence ID" value="XM_007372643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGF4"/>
    <dbReference type="FunCoup" id="G3AGF4">
        <property type="interactions" value="561"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGF4"/>
    <dbReference type="GeneID" id="18872478"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58513"/>
    <dbReference type="eggNOG" id="KOG1290">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_81_3_1"/>
    <dbReference type="InParanoid" id="G3AGF4"/>
    <dbReference type="OMA" id="CDPNKDM"/>
    <dbReference type="OrthoDB" id="2649at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042307">
        <property type="term" value="P:positive regulation of protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035617">
        <property type="term" value="P:stress granule disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000409">
        <property type="entry name" value="CMGC/SRPK protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051334">
        <property type="entry name" value="SRPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634:SF9">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="117"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="194"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="117"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="140"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW35293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="505" mass="55867" checksum="07BE88302E685D61" modified="2011-11-16" version="1">MVFEVLGENLLGLIRRYKHRGIPIVFVKQIAKQLLAALDFLHRSCGVIHTDLKPENILIEIGDVEAIVRIVEREDMKRKLQRKLSKLSNNSKKSSPLNSYQDHTLLSSSSAARILTPTHSATTMNSNSPSLGRSRRTRRSNTLITGSQPLPSPLRSFNKSFTNVYGLSSTTANTPIRTVTVPTSSNIAAFGSMGNSSIPHSTVEEESPQPQANEEGASLDNSLSSMSINNSYQPNVDPVKIPTMDSGSMYSSSINNNATTNDIIINEDELISVKIADLGNACWTTHHFTDEIQTRQYRSPEVILGYHWGASSDLWSFACLIFELLTGDYLFDPRDGKTYSKDDDHIAQIIELIGPFPREMLKESYYSHEFFNSRGELRRIVKLKPWSLKDVLSEKYKFSISDAIEIADFLRPMLEIQPEKRADAGGMINHSWLGDVLGLENVVLERPVGGSGEDIPGWSKEVTATNTTTTATSMPGKSISPSVIPMSGVNANATSGNNNGNFYYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGG2</accession>
    <name>G3AGG2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">pyridoxal kinase</fullName>
            <ecNumber evidence="2">2.7.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_58523</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pyridoxine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.35" evidence="2"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372713.1">
        <property type="nucleotide sequence ID" value="XM_007372651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGG2"/>
    <dbReference type="FunCoup" id="G3AGG2">
        <property type="interactions" value="301"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGG2"/>
    <dbReference type="GeneID" id="18872485"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58523"/>
    <dbReference type="eggNOG" id="KOG2599">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046496_1_0_1"/>
    <dbReference type="InParanoid" id="G3AGG2"/>
    <dbReference type="OMA" id="HTQYGQW"/>
    <dbReference type="OrthoDB" id="2104723at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008478">
        <property type="term" value="F:pyridoxal kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009443">
        <property type="term" value="P:pyridoxal 5'-phosphate salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01173">
        <property type="entry name" value="pyridoxal_pyridoxamine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004625">
        <property type="entry name" value="PyrdxlKinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00687">
        <property type="entry name" value="pyridox_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534:SF2">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="7">
        <location>
            <begin position="58"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="318" mass="35599" checksum="4C8463949AD9FD04" modified="2011-11-16" version="1">MKSLLSVQSHVVHGYVGGRAAIFPLQTQGWEVDNINTVNFSNHTGYGSFAGSSLAPEELDSILTQLETKLNVQYKALVSGYIPNADLIRTLSQHLPRLKRDGGYYLLDPVMGDNNYMYVDKSCIKEYQNILLLDDLVDIITPNQFELELLTGLRITCKLTLIQAINKLHQEYNIPYIVITSVSGEIFDSQTNDIFCVVSSSQGCKVFKVPMIKSYFTGVGDLFSALLVDRLEKNDMDLSTSVNQVLTIVSWTLKLTHKSALLAQRAAAKNENAGELVGKINDADTMKYLELRIIQARDYYSYDGPGEFVETDMDRLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGG4</accession>
    <name>G3AGG4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="7">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_146401</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Vacuole membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PI3/PI4-kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="7"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372715.1">
        <property type="nucleotide sequence ID" value="XM_007372653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGG4"/>
    <dbReference type="FunCoup" id="G3AGG4">
        <property type="interactions" value="257"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGG4"/>
    <dbReference type="GeneID" id="18870714"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_146401"/>
    <dbReference type="eggNOG" id="KOG2381">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009049_1_0_1"/>
    <dbReference type="InParanoid" id="G3AGG4"/>
    <dbReference type="OMA" id="WAYSPIS"/>
    <dbReference type="OrthoDB" id="3349449at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039756">
        <property type="entry name" value="Lsb6/PI4K2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12865:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12865">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE TYPE-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="9">
        <location>
            <begin position="212"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="653" mass="75948" checksum="64B69BC2B5EAE8A2" modified="2011-11-16" version="1">MKFKIPRELDVVNDQLRSNSVSPQSPLKRTEENGCTDNDDLATPQTPPESMAHLNTQFLSRSWHHSNEDSKHIPVKIKSASMVPVPLILPPAEPDLGSRSQPTSRRGSVEYDSNFRRFTRAYLIPAAKWAYSPLSKNKKKVKHDEDYIIEYSTFRPLRNLPQLTSARDIFIHWRRVVEDETKIPFDFPENYLSERDFSMLLSHITEIIELEHEFPQRITQGSSGSYFIYGRRPNGEIYKAGVFKPKDEEPYGPLSPKWSKWAHRTFFPCCFGRSCLIPNLGYISEAAACCLDRHLQSYIVPFTEVICLRSPTFFYSYWDQNSDVTKLPQKIGSFQVFLQGYSEADVWIRQNPIPSDHETISNLPKYSDVEVDINESEFTFKWSIESMRQFQEEVEKLVILDYIMRNTDRGLDNWMIRVDWTQVDSDSKTTKIMKPKIKIGAIDSGLAFPWKHPDEWRSFPFGWLFLPYSIIGQPFSMRTRNHYLPLLTSKLWWEQTVKGLRGVFTKDQDFQERLWHKQLAVLKGQAFNVVEILKLGYAGPLELAKRENLLVFDDVMYMPENIDSNEDIFRMQSSSYETDTSRQYDDIHTPLLPSQAHTYLSNSLVAINEDDVANGSGYEHINRDSGVNATGNRRVIIERLVKETSRPPVFTWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGJ6</accession>
    <name>G3AGJ6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58566</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372747.1">
        <property type="nucleotide sequence ID" value="XM_007372685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGJ6"/>
    <dbReference type="GeneID" id="18872504"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58566"/>
    <dbReference type="eggNOG" id="ENOG502TGMD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052376_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGJ6"/>
    <dbReference type="OMA" id="SLIMCGG"/>
    <dbReference type="OrthoDB" id="4078735at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033521">
        <property type="entry name" value="lasso_leader_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="348" mass="39786" checksum="D4CD116289BFEA55" modified="2011-11-16" version="1">MSINPNLQAFRINSNRNNNNNNIHNINNSDSDNNDHKNSFIRIRNKKSHKSRMNSRQNAANSPQLSKRPIQANYDTTSSILSDYEDLEFVEESSYVLFNPVQSNKRNESDILSLTNTTNTHAYSSDAEEDEGDESEDDYSIPERRELDEGSTTQLYTQQTEDHLSNKINSWYNSNNALLDDQLLISDNVASWNLDEAVMDENVTIDKTNPKDKTILKAFYGDELFKYLNPDEIARVKKFHKLTDIKDYLLDKENDSSTNYNSLLNQILYKLLLLDKHNDDDETDIAPEPETISQSPRFSGTTDYVNHLKRTRKIAIPTLVEVGTFSETTNGSSLIMCGGETTSSWNEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGX7</accession>
    <name>G3AGX7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Lactoylglutathione lyase</fullName>
            <ecNumber evidence="4 8">4.4.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glyoxalase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_57703</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(R)-S-lactoylglutathione = methylglyoxal + glutathione</text>
            <dbReference type="Rhea" id="RHEA:19069"/>
            <dbReference type="ChEBI" id="CHEBI:17158"/>
            <dbReference type="ChEBI" id="CHEBI:57474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="EC" id="4.4.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the glyoxalase I family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.5" evidence="4 8"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372062.1">
        <property type="nucleotide sequence ID" value="XM_007372000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGX7"/>
    <dbReference type="FunCoup" id="G3AGX7">
        <property type="interactions" value="216"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGX7"/>
    <dbReference type="GeneID" id="18872161"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57703"/>
    <dbReference type="eggNOG" id="KOG2944">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGX7"/>
    <dbReference type="OMA" id="MGDAWGH"/>
    <dbReference type="OrthoDB" id="16820at2759"/>
    <dbReference type="UniPathway" id="UPA00619">
        <property type="reaction ID" value="UER00675"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004462">
        <property type="term" value="F:lactoylglutathione lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07233">
        <property type="entry name" value="GlxI_Zn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004361">
        <property type="entry name" value="Glyoxalase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018146">
        <property type="entry name" value="Glyoxalase_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00068">
        <property type="entry name" value="glyox_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10374:SF30">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10374">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE GLYOXALASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00934">
        <property type="entry name" value="GLYOXALASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00935">
        <property type="entry name" value="GLYOXALASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="VOC" evidence="9">
        <location>
            <begin position="9"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="VOC" evidence="9">
        <location>
            <begin position="162"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="324" mass="37102" checksum="3A55F61DDF2C3FF0" modified="2011-11-16" version="1">MPSIDNSFLMNHTCLRIKDPKVTIPFYTEGLGFKLIKTFPFETFTLYMLNYENESNKDLNWSAREGVLELCHNHGVENDPDYKLNHGNGTEFRGFGHICVTVDNIEVVQEKLLAKGVKFQKKLSDGRQKNIAFALDPNGYWIELVENGINKVEAKTDSANYRFNHSMIRVKDPKKSLDFYKNVLGFKIISKKDFPEAKFTLYFLGYDHDPEFKEDSQQGNEQMKRSSLIELTHNWGTESDPDFKGYHNGNSTENGAIQGYGHTCVSCKDPAKFCAEIEQELGDKADWSLKWDQGNIKKIAFIRDPDGYSVEILGHDLFANQPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH70</accession>
    <name>G3AH70_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_58740</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372913.1">
        <property type="nucleotide sequence ID" value="XM_007372851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH70"/>
    <dbReference type="STRING" id="619300.G3AH70"/>
    <dbReference type="GeneID" id="18872580"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58740"/>
    <dbReference type="eggNOG" id="KOG0143">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010119_6_2_1"/>
    <dbReference type="InParanoid" id="G3AH70"/>
    <dbReference type="OMA" id="FTTRHDP"/>
    <dbReference type="OrthoDB" id="10537583at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044283">
        <property type="term" value="P:small molecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000051">
        <property type="entry name" value="Clavaminate synthase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="2">
        <location>
            <begin position="210"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="372" mass="41860" checksum="EF2B01A3B3509215" modified="2011-11-16" version="1">MSSKHPLSEIGILFKDALFKLINQQYAHGSKNFHFNITKMSNPLKIVDISQDDQALVQKQLLDAATTQGFLFIEGHDFTQQEVDSLFELSNEFFKLPHEYKQKFPIDASNHGYSDFAGENLDPKNQKKGDPKEALNFCALNFKTGDSSKPIPDWFTNDSSREELLRGVIVRLYKLSIRILEILAQGLEIEDTDGLKGKDWFSAKYAPDKSSGSTFRLLHYPGQKSLNPESVVRAGAHTDYGAVTLLFQRENQAGLEIYSPVSKQWESVPFVPSNDAGVAPPIVVNIGDLLSYWTAGLLKSTIHRVKFPKEVQETGQDRYSIVFFSHPNDDALLEPVPCSVIRNREGRGANNAEVKITAREHLQRRLAATYGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHD1</accession>
    <name>G3AHD1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_59517</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373679.1">
        <property type="nucleotide sequence ID" value="XM_007373617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHD1"/>
    <dbReference type="FunCoup" id="G3AHD1">
        <property type="interactions" value="139"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHD1"/>
    <dbReference type="GeneID" id="18872953"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59517"/>
    <dbReference type="eggNOG" id="KOG0698">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013173_0_7_1"/>
    <dbReference type="InParanoid" id="G3AHD1"/>
    <dbReference type="OMA" id="CIYVANT"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF803">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="255" mass="29005" checksum="A351C99A4EEAE844" modified="2011-11-16" version="1">MNHNETFKVLKNAFFKIDHDLNKHLSLINCGSTAIVTTIINNCIYVANTGDSRCILSLDGGNAKTLSFDHKPSNMGERVRIENSNGYILNGRINEVLALSRAFGDFKFKLPYINSTRNKYILENNKRIKKDLVHLPPELFQVTVEPDILLYDMNFNKIPEFIVIACDGVWDCFKNGQLIKLIRDKLALGWKLNKIVEFVLNDCLTMANNYTGIGFDNMTMIIVALHPDGNVAGDNNLDEWYQVMMDKILKEKGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHJ3</accession>
    <name>G3AHJ3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="3 7">2.7.7.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">UGP1</name>
        <name evidence="10" type="ORF">SPAPADRAFT_134841</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a central role as a glucosyl donor in cellular metabolic pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>alpha-D-glucose 1-phosphate + UTP + H(+) = UDP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UDPGP type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="3 7"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373741.1">
        <property type="nucleotide sequence ID" value="XM_007373679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHJ3"/>
    <dbReference type="FunCoup" id="G3AHJ3">
        <property type="interactions" value="921"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHJ3"/>
    <dbReference type="GeneID" id="18869851"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134841"/>
    <dbReference type="eggNOG" id="KOG2638">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023632_3_0_1"/>
    <dbReference type="InParanoid" id="G3AHJ3"/>
    <dbReference type="OMA" id="KEYCFLS"/>
    <dbReference type="OrthoDB" id="932129at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-alpha-D-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00897">
        <property type="entry name" value="UGPase_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000001">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000002">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002618">
        <property type="entry name" value="UDPGP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016267">
        <property type="entry name" value="UDPGP_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43511">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01704">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000806">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 10" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000806-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000806-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="502" mass="55961" checksum="F1DF515148A6A024" modified="2011-11-16" version="1">MSSAAAPKKHQKTQSTYAFDTTATTVAASQMRNALNKLADTVEDEEKQQRFENEMDSFFSLFRRYLTEKASGTTLDWEKIRSPSADEVVAYSKLPELENKNNLSKLAVLKLNGGLGTSMGCVGPKSVIEVRDGNTFLDLSVRQIEHLNRHYDADVPLLLMNSFNTDKDTEKIIKKYQGHRIRVKTFNQSRFPRIYKDSLLPVPESYDDSLESWYPPGHGDLFESLVQSGELDALLAQGREILFVSNGDNLGATVDTKILDHMIETGAEYIMELTDKTRADVKGGTLINYEGQVRLLEIAQVPKEHVEEFKSIKKFKYFNTNNLWINLRAIKELVESNAIEAEIIPNQKTISKGSSDINVLQLETAVGAAIRHFKGAHGVVVPRSRFLPVKTCSDLLLVKSDLFYLESGALKLDPTRDGFANPLIKLGSHFKKVSGFQSRIPHMPKILELDHLTITGNVTLGRGVTLKGTVIIVCNDGEKIDIPNGSILENVVVTGNLTILEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHS5</accession>
    <name>G3AHS5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_148821</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373823.1">
        <property type="nucleotide sequence ID" value="XM_007373761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHS5"/>
    <dbReference type="STRING" id="619300.G3AHS5"/>
    <dbReference type="GeneID" id="18870800"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_148821"/>
    <dbReference type="eggNOG" id="KOG0802">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_683550_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHS5"/>
    <dbReference type="OMA" id="LYFYTFP"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16448">
        <property type="entry name" value="RING-H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050731">
        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057992">
        <property type="entry name" value="TPR_SYVN1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25563">
        <property type="entry name" value="TPR_SYVN1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="85"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="319"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="363" mass="42683" checksum="A81AD2A02B8E79A4" modified="2011-11-16" version="1">MSTTSWRIRIGGAFGAVLGYSAYKRLKAQGSKPNLHQYFNTLVSLRTRFNPGVQFFVLFLCLNFLKWITFGKLTPREIKNLKDKIAYTIWEFFFGFMILYFKIGSYSIIQVEVFKFAGLFFTIILLKGFHYLSADRIQTVFNLSHSTPTVKLWAIKLSIGLIMLNLIDAMLIVKFFYELYHSSTNEKGLSMVDNILVGIFGFEILNLFPVIVLTTLKCGLELSQYLNEDEQEDTQWREYKLKWIYMGEFIVNLIRFIMVCLFSVAFLYFYTFPIHILPSSYLSLRVLVAKTRNFINFKKKQFVLEKLTVPTEVFRDEKCVVCFEEFTDVNDIRQLNCTHSFHYRCLKSWIYYSNSCPTCREVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHU2</accession>
    <name>G3AHU2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small ribosomal subunit protein uS4m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_59680</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373840.1">
        <property type="nucleotide sequence ID" value="XM_007373778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHU2"/>
    <dbReference type="FunCoup" id="G3AHU2">
        <property type="interactions" value="141"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHU2"/>
    <dbReference type="GeneID" id="18873022"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59680"/>
    <dbReference type="eggNOG" id="ENOG502QTS9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026386_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHU2"/>
    <dbReference type="OMA" id="GDMFQVE"/>
    <dbReference type="OrthoDB" id="3356781at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000025">
        <property type="entry name" value="30S ribosomal subunit S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_uS4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF4">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US4M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 10" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RNA-binding S4" evidence="11">
        <location>
            <begin position="104"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW34256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="462" mass="52582" checksum="A033F9C8191EF71D" modified="2011-11-16" version="1">MPRKADRLHTLARGRVRMSMNKKNLYNLIKKPEATYTGKTLYQQKYLAKTETRGYHGEHINETRWKQLFSPGLKSNVQLDATMRGAAGQKHTPMVLQTFSPLEKRLEIAIFRAMFASSVRQARQFIMGGHVSVNGVKIKHPSFPLKSGDVFHVDPVKVLYALGKSKPSLEQALEIDNSQITQYNNYVDRAQADSKGEWEKLQKKNSLNSIANLEKKEREKLELVEKRKQMKSAQDEITRESILLDILQLGSAATAPVTAETFAKYGPHNSKKCSDIFQKVSKNAKDIVEKPTLASVKEFVNPDETQKANQPDSVKHVARSIKAQLTELQKSQWAEIRREIESAENTGAAPYDPTYASKLTKVPLLNKEEILEDESNAVVELPWQKHLHGRQDPSKPYFTPWSPRGFLGCFSILPHHIEVSFDTCHAVYLRDPIARPGHSEVISPSPEHVHERAYMYYRRKGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI43</accession>
    <name>G3AI43_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">G4 quadruplex nucleic acid binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_59776</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373941.1">
        <property type="nucleotide sequence ID" value="XM_007373879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI43"/>
    <dbReference type="FunCoup" id="G3AI43">
        <property type="interactions" value="345"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI43"/>
    <dbReference type="GeneID" id="18873072"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59776"/>
    <dbReference type="eggNOG" id="KOG2241">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009710_6_6_1"/>
    <dbReference type="InParanoid" id="G3AI43"/>
    <dbReference type="OMA" id="QVCRTVC"/>
    <dbReference type="OrthoDB" id="19141at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017102">
        <property type="term" value="C:methionyl glutamyl tRNA synthetase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006418">
        <property type="term" value="P:tRNA aminoacylation for protein translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10304">
        <property type="entry name" value="GST_C_Arc1p_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02799">
        <property type="entry name" value="tRNA_bind_EMAP-II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000199">
        <property type="entry name" value="tRNA-aminoacylation cofactor ARC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053836">
        <property type="entry name" value="Arc1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF33">
        <property type="entry name" value="AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21972">
        <property type="entry name" value="Arc1p_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="5">
        <location>
            <begin position="208"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="134"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="134"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="374" mass="40971" checksum="1D83C318D799141F" modified="2011-11-16" version="1" fragment="single">MSSLISQFQKVRLSEVSSVDASKFPTLNQEEQALASQFETLSGRFDEAESLTVLNDSLRSKTYVAGTVPSKSDLVVFEKVFPLAGKWTAVENIAKYRHILRWVDLVQNTLVDVPEADKIKIDYNVEIPREIKEKEKKKPAEGQAKEAAAAAPAAAAAATGAAPNGKKELTEEEKKARAEAQKAKKAAKAKANAEKQAAAAAAANVPANPSMIDFRVGFIQKAEKHPNADSLYMSTIDMGDEDGPRIVCSGLVNYIPIEEMQQRYVIVIANLKPVTMRGVKSCAMVLCASEEGKVEFVNPPPGSKAGDKIFFEGFNGTPEKQLNPKKKIWEACQERFSTNENFEVTYTEEGKEPKRLVNEKGELCKNSTIVKAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI72</accession>
    <name>G3AI72_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="13">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_149190</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. RAD25/XPB subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="13"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373969.1">
        <property type="nucleotide sequence ID" value="XM_007373907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI72"/>
    <dbReference type="FunCoup" id="G3AI72">
        <property type="interactions" value="1008"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI72"/>
    <dbReference type="GeneID" id="18870815"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_149190"/>
    <dbReference type="eggNOG" id="KOG1123">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008213_0_0_1"/>
    <dbReference type="InParanoid" id="G3AI72"/>
    <dbReference type="OMA" id="RCQEIDY"/>
    <dbReference type="OrthoDB" id="10262986at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000112">
        <property type="term" value="C:nucleotide-excision repair factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015616">
        <property type="term" value="F:DNA translocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000019">
        <property type="term" value="P:regulation of mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001111">
        <property type="term" value="P:RNA polymerase II promoter clearance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001113">
        <property type="term" value="P:transcription open complex formation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001174">
        <property type="term" value="P:transcriptional start site selection at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18029">
        <property type="entry name" value="DEXHc_XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18789">
        <property type="entry name" value="SF2_C_XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000077">
        <property type="entry name" value="Probable DNA repair helicase RAD25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000117">
        <property type="entry name" value="Putative DNA repair helicase rad25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050615">
        <property type="entry name" value="ATP-dep_DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032438">
        <property type="entry name" value="ERCC3_RAD25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001161">
        <property type="entry name" value="XPB/Ssl2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032830">
        <property type="entry name" value="XPB/Ssl2_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00603">
        <property type="entry name" value="rad25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274">
        <property type="entry name" value="RAD25/XP-B DNA REPAIR HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16203">
        <property type="entry name" value="ERCC3_RAD25_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13625">
        <property type="entry name" value="Helicase_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00851">
        <property type="entry name" value="XRODRMPGMNTB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 18" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="367"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="583"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="19"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW34385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="842" mass="95439" checksum="344304043E693304" modified="2011-11-16" version="1">MSSSRRTGEVNYAELNNGDYDDDFLSDDKISDEDEYGAKPRKRKNKSSTVDSQAKRKQAQSTVDYIRNEVDDTNFSDEELIELTPDIPADYVPDAVSKAFGKADFSYLKLKPDHFSRPIWISPNDGRIILESFSPLAEQAQDFLITIAEPISRPSHIHEYRITAYSLYAAVSVGLETDDIISVLNRLSKVPVAESIINFIKGATVSYGKVKLVLKHNRYFVESTQADILQMLLKDPVIGPLRIQSSENTVTSNGLVQSAAPSNDIVIPGTKKPEQDKNNNTEAEDIFASVVGNKGEEDNDDDDLDTVHSFEISNDSVEIVKKRCQEIEYPVLEEYDFRNDARNADLEIDLKPSTQIRPYQEKSLSKMFGNGRARSGIIVLPCGAGKTLVGITAACTIRKSVIVLCTSSVSVMQWRQQFLQWCTIQPENVAVFTSENKEMFTSDSGLVVSTYSMVANTRNRSHDSQKVMDFLRSREWGFIILDEVHVVPAAMFRRVVTTIAAHAKLGLTATLVREDDKIDDLNFLIGPKLYEANWMDLAQKGHIANVQCAEVWCPMTSEFYQEYLRESARKRMLLYIMNPTKFQACQFLIHYHEKRGDKIIVFSDNVYALQEYALKLGKPFIYGSTPQQERMKILQNFQHNDQINTIFLSKVGDTSIDLPEATCLIQISSHYGSRRQEAQRLGRILRAKRRNDEGFNAFFYSLVSKDTQEMYYSTKRQAFLVDQGYAFKVITHLSGMEQLPDLAYASPRERRELLQQVLLKNEDAAGLEVGDDVDTNFISKEKRMKLERQQGSGATRTAGSLAGLAGGEDMAYIEYSKNKNKELRDQHHPLIQKMYYPKQGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI82</accession>
    <name>G3AI82_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kinetochore protein NDC80</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_49441</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the NDC80 complex, which consists of NDC80, NUF2, SPC24 and SPC25.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Chromosome</location>
            <location evidence="11">Centromere</location>
            <location evidence="11">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the NDC80/HEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373980.1">
        <property type="nucleotide sequence ID" value="XM_007373918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI82"/>
    <dbReference type="FunCoup" id="G3AI82">
        <property type="interactions" value="295"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI82"/>
    <dbReference type="GeneID" id="18871336"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49441"/>
    <dbReference type="eggNOG" id="KOG0995">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012583_1_0_1"/>
    <dbReference type="InParanoid" id="G3AI82"/>
    <dbReference type="OMA" id="NKSWLMT"/>
    <dbReference type="OrthoDB" id="7459479at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.30:FF:000001">
        <property type="entry name" value="Probable kinetochore protein ndc80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.30">
        <property type="entry name" value="Ncd80 complex, Ncd80 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005550">
        <property type="entry name" value="Kinetochore_Ndc80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055260">
        <property type="entry name" value="Ndc80_CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038273">
        <property type="entry name" value="Ndc80_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10643">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10643:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03801">
        <property type="entry name" value="Ndc80_HEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="10 11" id="KW-0137">Centromere</keyword>
    <keyword evidence="3 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5 11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Ndc80 CH" evidence="14">
        <location>
            <begin position="132"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="49"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="104"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="317"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="390"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="552"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="145"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW34396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="688" mass="78355" checksum="037EF6D050E2DBB7" modified="2011-11-16" version="1">MAPPRLFQNDPIDSSGTKLASILRTVESSSRRTNSLTPVTKRGVKRRSLIASHTHERGSIENDRRESVFFSTPARVNKKRPSPGPSFIKARPQLHTHTAALTNTSSSFMPSSSSVAGPQQQSLRSTPALSSRASAAQAPLGGGSSLVTPSSQQSLAQRDPRPLRDRKYQELIQAEIHSFLTENRFELEPSIKLTESTLKTPTQKEFTGIFSFLVKMQNPSFKLGKAPDTEIYTALKVMNYPYLDSISRSQIGAVGGQNWPTFLGVLYWLVTVNLKLLRITEEDETPEDEDRLHDEYHAYISSKYARYLAGRDNENDEIELQEKLDRMKVSAEEQLQEYSHNKNAIEEAVVHLEADGKIIETAVKKSEDLYQDFDALKNYIESRKNSKPKYDEILANLKQAAENESRLLEEARVKVVELQAQLEQLGISQEFIQGLRDNRDQADKEFADAEEQYMKLREDSITAHEHFARDIDRLKQSVRDLNSLSQLFNPDFQLELNKDIKTSNTFTRALKPDEILTRKTSEVKIEFVSLKSRLTSLHGEYRDTSDQLKTERDKITDVISLLESKRDEARRKVDALQREYDREYASMQQKQAENKVEIARLETDLVTRQSDARNSLLNLEKANKDAREKHNKIVADISSKRSILNSVRSEAGAIALKVQTNTQENLLRLDDKVQEESIVESDLRNELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIB2</accession>
    <name>G3AIB2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mitochondrial ribosomal protein MRPL25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_134611</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373265.1">
        <property type="nucleotide sequence ID" value="XM_007373203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIB2"/>
    <dbReference type="FunCoup" id="G3AIB2">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIB2"/>
    <dbReference type="GeneID" id="18869837"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134611"/>
    <dbReference type="eggNOG" id="ENOG502S29G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076154_2_0_1"/>
    <dbReference type="InParanoid" id="G3AIB2"/>
    <dbReference type="OMA" id="KGHKHEL"/>
    <dbReference type="OrthoDB" id="18529at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037507">
        <property type="entry name" value="Ribosomal_mL59"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040922">
        <property type="entry name" value="Ribosomal_mL59_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28041">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28041:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18126">
        <property type="entry name" value="Mitoc_mL59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein mL59" evidence="1">
        <location>
            <begin position="15"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="156" mass="18915" checksum="983AA92FF7F50E52" modified="2011-11-16" version="1">MSLTPQQAFKKLPAKLHNFFLRYPPRPYAQYAAGKSTIDDPQRNPFFPNKNTTNGRWYDATYSRRRSADLFKTAYKFGLQDLLPPMPRKFYQDKFDNKNWMRGVLHQKKQKWERELPAKLEARKEAIENMDAIILEKRPRYKRQLEKQQQKKKTWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIC3</accession>
    <name>G3AIC3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_134437</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373277.1">
        <property type="nucleotide sequence ID" value="XM_007373215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIC3"/>
    <dbReference type="FunCoup" id="G3AIC3">
        <property type="interactions" value="643"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIC3"/>
    <dbReference type="GeneID" id="18869816"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134437"/>
    <dbReference type="eggNOG" id="KOG4391">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029375_2_0_1"/>
    <dbReference type="InParanoid" id="G3AIC3"/>
    <dbReference type="OMA" id="QYWTSED"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF81">
        <property type="entry name" value="PROTEIN ABHD13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="112"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="289" mass="32647" checksum="67B73AEC12FECEA9" modified="2011-11-16" version="1">MESIFRIAKSMAAIGITVAALALGGLYTYQSSIIYPSSMNGARAHVSTPDEYGMEYEAVKLTTPDGETLECFSLKQDITNNYTNKTILILSPNAGNIGYALPIVQLLYARFRYNVFIYSYRGYGNSTGTPSEKGLKIDADCVMEYLKSDKQYSSSKIVLYGRSLGGAVAIYIAEKYGDLVSGIILENTFLSIPKTVPHVFPLLKYFTLFVNQIWDSESLVPRINENIPVMLMSARQDEIVPPSHMDEIYKKLPSEIKIKWKYEQSTHNDTVVQDEYWERVEEFVNEKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIH6</accession>
    <name>G3AIH6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DUF814 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_70556</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NEMF family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374030.1">
        <property type="nucleotide sequence ID" value="XM_007373968.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIH6"/>
    <dbReference type="FunCoup" id="G3AIH6">
        <property type="interactions" value="1022"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIH6"/>
    <dbReference type="GeneID" id="18875333"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70556"/>
    <dbReference type="eggNOG" id="KOG2030">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003612_1_1_1"/>
    <dbReference type="InParanoid" id="G3AIH6"/>
    <dbReference type="OMA" id="XIEKVLV"/>
    <dbReference type="OrthoDB" id="207084at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904678">
        <property type="term" value="F:alpha-aminoacyl-tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140708">
        <property type="term" value="P:CAT tailing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043043">
        <property type="term" value="P:peptide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021846">
        <property type="entry name" value="NFACT-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051608">
        <property type="entry name" value="RQC_Subunit_NEMF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239">
        <property type="entry name" value="NUCLEAR EXPORT MEDIATOR FACTOR NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239:SF6">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11923">
        <property type="entry name" value="NFACT-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="7">
        <location>
            <begin position="423"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="NFACT protein C-terminal" evidence="8">
        <location>
            <begin position="748"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="298"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="615"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="689"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="298"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="335"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="630"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="640"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="651"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="865" mass="98401" checksum="5D82A713273A6821" modified="2011-11-16" version="1">MSSAKEDVDRYAVREEYKMFDESLFSEQFQYAKREYSPDDIAKWIETHKSKISESSDSKKGKVYSIHKLAFLNASHLSSELIQKVLHENDIDPAQSCLRFEQDKEGLQSVANALGACEDAYLSLVDSKNENTGFIVAKRNKASDTNSSFEFIYDEFHPFKPYKANQEDYQYTEVSGYNKTLDRFFSTLESSKFELKVEQLKQTAAKRLDKAKSERDKQIQSLLEQQDLNAKKGELIQYHADLVDDCRAYIQSFLDQSMDWTNIETVLELEKKKNNKLAKAIGLPLNLKDNKIKVLLPEFEDPESEENSDSDSDSETDSESESGSESEDEDNFPKRVSKKPANKKEKKSNVVPTWIDLSLTSFANARAYFDTKKTAATKQEKVEKSTKIALKNAERKITHDLTKNLKQENDSLKQMRPKYWFEKFFWFVTNEGYLCLAGRDNAQVDMIYYRHFSDNDCFVSSDLEGSLKVFVKNPLKGETVPPSSIMQAGIFAMSASSGWNGKVTTSAWVLHGDEISKRDFDGSLIASGEFRYTGKKVYLPPAQLVMGFGLYSLVDEESTKRYADARTSREKEHGFNIVMDNKKRELDMLKGSKEATPVGEADKSNKDATLDQAGSAFEKESEGETVPSEDASEITESDLESVTASEATASKKNKPLSRGKRSKLKKIAAKYADQDEEERRLRMDALGTLKQIEQKEQQTKREVLEKMEATKRMQEMQAVRERRKKQDEKEYQKYLSNEVDSDESHVTNYLEILDSFAPKPSTKDEIISMVPVFAPWISLQKFKYKVKIQPGSGKKGKCIGDSLNYFTTRKMDPSSTDTDLDWPQERDLVKGLKTNDLVGVFTVSKVKLVLPGGSDNNKKKGGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIU9</accession>
    <name>G3AIU9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_64989</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373344.1">
        <property type="nucleotide sequence ID" value="XM_007373282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIU9"/>
    <dbReference type="GeneID" id="18875009"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64989"/>
    <dbReference type="HOGENOM" id="CLU_1035012_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIU9"/>
    <dbReference type="OrthoDB" id="4027837at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="269" mass="32704" checksum="BAB2A70E8B833981" modified="2011-11-16" version="1">MAFKKRYNSKKNYQGNNNNRDWSNNYSRPLSAVEIQRKKNQESSDISIRYSEIFDGTAKTLKWFEIVCKQIEIKCLRYISQHNLKIEISELNVIAKCHLVDGAGLWLEHFLRPYYKEMTDETGKIIPEKIYMDYNEFIKAFRNEYEDDQVKFELSLEVGTYYQKRQSLNEYRQGFRNILNRYKQEQIEEQENYLVNKFRRNANTEYQPILVSIKTYKDIDNLMEDPEIENRVRRTYKKPFNYNQRNYQNGNRNNNYNNNFKKNNKGKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIZ2</accession>
    <name>G3AIZ2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_48919</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373387.1">
        <property type="nucleotide sequence ID" value="XM_007373325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIZ2"/>
    <dbReference type="FunCoup" id="G3AIZ2">
        <property type="interactions" value="286"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIZ2"/>
    <dbReference type="GeneID" id="18871286"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48919"/>
    <dbReference type="eggNOG" id="KOG0062">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1242">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002848_0_1_1"/>
    <dbReference type="InParanoid" id="G3AIZ2"/>
    <dbReference type="OMA" id="EDHRKFG"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008079">
        <property type="term" value="F:translation termination factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006449">
        <property type="term" value="P:regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18626">
        <property type="entry name" value="CD_eEF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.990:FF:000002">
        <property type="entry name" value="mRNA export factor elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000193">
        <property type="entry name" value="Probable Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015688">
        <property type="entry name" value="eEF3_ABC2_chromodomain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047038">
        <property type="entry name" value="eEF3_chromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056891">
        <property type="entry name" value="NEW1_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF14">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24984">
        <property type="entry name" value="HEAT_EF3_GNC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24987">
        <property type="entry name" value="HEAT_EF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24988">
        <property type="entry name" value="NEW1_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="576"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="819"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="8">
        <location>
            <begin position="949"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="101"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1127"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="70"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1166"/>
            <end position="1176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1203" mass="134510" checksum="F81D3C570640DEAA" modified="2011-11-16" version="1">MSKQSFDDFLKQQQASQNKPKGYGYRNQQGNPSFQPQGYNQNFQQGYQQGFQPQGYNNNYYAQGYDPSFQQGTNYNQGHNQGYNQGYNQGYNQNYQRYNKNTRGPYQQESPITTPIESLPTSGRSTPMNPNASTTSLTSLNTALSKLNVENTPISENLSNIEKATKISEVRPEVDAIVDAIASQDSLSVINEWKLNEIVKSLLKAKNPALVREAALMIIQHLATKFGGQTPNEAYLLQFLSTAYDMFADKDKNVTKAAKSAVDALYGIYPVEALGSIVLDEYLNYFKSSAKWNSKVGALVNFDRIIEEVPSDLLELKFINAVPVLTDLSTDFKPELAKHGLATLKKFVKVLDNLDLQNKYDIIVDTLADPQKVTECIKNLSSVTFVAEVTEPALSLLVPILDKSLKMSSSSNEQLRQTVMVTENLTRLVNNKREIESFIPILLPGVEKVVNNASLPEVRELAGKALKVIQDAESEQTDGKFHGRITLEQAKDFYTENIPAELSSVVEKLNLDDEVIGNYLSIVLQADAHVNDWKRLQEYLELAVSEPKYAEAVVTNLRHLYNPESSTDNQDDDGAIEIVNADFSLAYGSRMLLNKTNLRLLKGHRYGLCGRNGAGKSTLMRAISKGQLEGFPSADELRTCFVEHKLQGSEADMDLVSFIASDPDLAEVHDRHDIEKALLEVGFTDERLQQNVGSLSGGWKMKLELARAMLLKADVLLLDEPTNHLDVTNVQWLQDYLVDHTSITSLIVSHDSGFLDAVCTDIIHYENKKLAYYKGNLSEFVKVKPEGKSYYTLTDSNVKMAFPPPGILTGVKSNTRSVARMTDVTFTFPGGAKPSLKNVSVSLSLSSRVAILGPNGAGKSTLIKLLTGELVPDSGKVEKHPNLRIGYIAQHALQHVEQHKEKTANQYLQWRYRFGDDREVLLKESRKVSDEELELMKKEIDIADGRGPRAIEAIVGRQKLKKSFQYEIKWKFWLPKYNSWVPREVLLEHGFDKLIQKFDDHEASREGLGYRELTPSVIRKHFEDVGLDGDIADHTPMGSLSGGQLVKVVIAGAMWNNPHLLVLDEPTNYLDRDSLGGLAVAIREWNGGVVMISHNSEFVGALCPEQWHIKDGEVVQKGTVGVDANRFEDGAESKEGSPAPTPAPKRADDDDSPANIKVRTRKKKMTRNEKKVQAERRRLRYLEWLSSPKGTPRPPDTDDEEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ56</accession>
    <name>G3AJ56_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Chitin synthase</fullName>
            <ecNumber evidence="2 10">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_59174</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Polymerizes chitin, a structural polymer of the cell wall and septum, by transferring the sugar moiety of UDP-GlcNAc to the non-reducing end of the growing chitin polymer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + UDP-N-acetyl-alpha-D-glucosamine = [(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9595"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="2.4.1.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the chitin synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2 10"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373397.1">
        <property type="nucleotide sequence ID" value="XM_007373335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ56"/>
    <dbReference type="FunCoup" id="G3AJ56">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ56"/>
    <dbReference type="GeneID" id="18872806"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59174"/>
    <dbReference type="eggNOG" id="KOG2571">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004760_3_2_1"/>
    <dbReference type="InParanoid" id="G3AJ56"/>
    <dbReference type="OMA" id="NTIMSWF"/>
    <dbReference type="OrthoDB" id="26569at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF9">
        <property type="entry name" value="CHITIN SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01644">
        <property type="entry name" value="Chitin_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="361"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="436"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="487"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="613"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="652"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="693" mass="79138" checksum="DFC0633DD44D091C" modified="2011-11-16" version="1">MYNEDDVLLGRTLKGVFKNIKYLESKSRSSVWGKDSWKKIVVCIVSDGRTKINERAQALLAGLGVYQEGLAKSMVDDKKVQAHIYEYTTRVGISCVDDETVKLTTEKIVPVQMLFCLKETNAKKINSHRWCFQAIGQVLDPKIVVLLDCGTQPSGKSLYKLWKEFDRDHRVAGACGEITASLKKRQMITNPIVYGQNFEYKISNILDKPTESVFGFISVLPGAFSAYRYVALQNDVNGIGPLEKYFKGEFLHSSGELDPNDDEYEMKRAMMKEEAGIFTSNMYLAEDRILCFELVAKRGCNWLLRYCKSASAETDVPDKLAEFILQRRRWLNGSFFAAIYALVHFAKVWRSSHSFGRKIFLHIEFFYQFINLVVSWFSIGSYFLVFRILTTSLGDPDLNFAPGNILSVVFLWLYLLSLVTTFVLSFGNKPKGTERFYVVIVVFFAILMAYMIFAAVFMAVHAVQGIYDNKTQITVSLFFQNAEFRNLVVATASTYALYFLASFLYFEPWHMFTSFLQYILLSPSYINVLNIYAFCNIDDVSWGTKGDTGGASLGEAKLREDGTFDVSVPILKEEINQSYLNQLEKIRDPAPKNDKPAEISNEDYYAYVRSMTVLIWMISNFVVVALVLQTGGFNQFEKIDHLQDSKTFRARVFLTVILWTVAFMALFRFLGCVGYLITRLGRRMKPSEHATKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJI5</accession>
    <name>G3AJI5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SUMO-conjugating enzyme UBC9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Ubiquitin carrier protein 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Ubiquitin-conjugating enzyme E2-18 kDa</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_59269</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373472.1">
        <property type="nucleotide sequence ID" value="XM_007373410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJI5"/>
    <dbReference type="FunCoup" id="G3AJI5">
        <property type="interactions" value="1226"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJI5"/>
    <dbReference type="GeneID" id="18872843"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59269"/>
    <dbReference type="eggNOG" id="KOG0424">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_12_0_1"/>
    <dbReference type="InParanoid" id="G3AJI5"/>
    <dbReference type="OMA" id="TWECGIP"/>
    <dbReference type="OrthoDB" id="6600758at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019789">
        <property type="term" value="F:SUMO transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000022">
        <property type="term" value="P:mitotic spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23798">
        <property type="entry name" value="UBCc_UBE2I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000035">
        <property type="entry name" value="SUMO-conjugating enzyme ubc9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="13">
        <location>
            <begin position="3"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="11">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00081544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW33888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="157" mass="18140" checksum="BE0548B7190ED6F5" modified="2011-11-16" version="1">MSLNQIRLQEERKQWRKDHPFGFYAKPMKTADGSLDLRQWTAGIPGKQNTLWANAVYPITIEFPEEYPSKPPKVRFPANFFHPNVYPSGTVCLSILNEEQDWRPAITLKQIVLGVQDLLDTPNDESPAQEPAYRAYKKDRALYEKKVKEQAKKYASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJN0</accession>
    <name>G3AJN0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leucine carboxyl methyltransferase 1</fullName>
            <ecNumber evidence="3">2.1.1.233</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Protein phosphatase methyltransferase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">[Phosphatase 2A protein]-leucine-carboxy methyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_59315</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[phosphatase 2A protein]-C-terminal L-leucine + S-adenosyl-L-methionine = [phosphatase 2A protein]-C-terminal L-leucine methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:48544"/>
            <dbReference type="Rhea" id="RHEA-COMP:12134"/>
            <dbReference type="Rhea" id="RHEA-COMP:12135"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90516"/>
            <dbReference type="ChEBI" id="CHEBI:90517"/>
            <dbReference type="EC" id="2.1.1.233"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. LCMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.233" evidence="3"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373515.1">
        <property type="nucleotide sequence ID" value="XM_007373453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJN0"/>
    <dbReference type="FunCoup" id="G3AJN0">
        <property type="interactions" value="544"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJN0"/>
    <dbReference type="GeneID" id="18872862"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59315"/>
    <dbReference type="eggNOG" id="KOG2918">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031312_1_0_1"/>
    <dbReference type="InParanoid" id="G3AJN0"/>
    <dbReference type="OMA" id="FITECVM"/>
    <dbReference type="OrthoDB" id="203237at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018423">
        <property type="term" value="F:protein C-terminal leucine carboxyl O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016651">
        <property type="entry name" value="LCMT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007213">
        <property type="entry name" value="Ppm1/Ppm2/Tcmp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13600">
        <property type="entry name" value="LEUCINE CARBOXYL METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13600:SF21">
        <property type="entry name" value="LEUCINE CARBOXYL METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04072">
        <property type="entry name" value="LCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016305">
        <property type="entry name" value="LCM_mtfrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="186"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR016305-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="264" mass="30292" checksum="FC085AD84EB8FC22" modified="2011-11-16" version="1" fragment="single">MLSPKDKQDKLIRATDLDALSCRNSINIKSYLTPNDIYIPKLIESYRQNLQYCYGYTNLSSSRALHLFNDRKLPLINRGTYLRTKAIDNIVQGFIEELDKCQIVSLGSGSDTRAFSILNKYSNVIYHEIDFPESVKIKKLAIYNDDELRKTVGLGSDTIPMIKSRDEFVQLDCDLQTSRYHLHGIDIRTWKDNKTPFAHFDSNLPTLVISECSLCYLAPDEYENTINYLTGISKNLISFIIYEPMSLNDSFGLTMTKNLLDRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJP9</accession>
    <name>G3AJP9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">3-ketodihydrosphingosine reductase TSC10</fullName>
            <ecNumber evidence="14">1.1.1.102</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">3-dehydrosphinganine reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">KDS reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">SPAPADRAFT_59342</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the reduction of 3'-oxosphinganine (3-ketodihydrosphingosine/KDS) to sphinganine (dihydrosphingosine/DHS), the second step of de novo sphingolipid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>sphinganine + NADP(+) = 3-oxosphinganine + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22640"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57817"/>
            <dbReference type="ChEBI" id="CHEBI:58299"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.102"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="19">
            <dbReference type="Rhea" id="RHEA:22642"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.102" evidence="14"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373534.1">
        <property type="nucleotide sequence ID" value="XM_007373472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJP9"/>
    <dbReference type="FunCoup" id="G3AJP9">
        <property type="interactions" value="121"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJP9"/>
    <dbReference type="GeneID" id="18872876"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59342"/>
    <dbReference type="eggNOG" id="KOG1210">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_3_0_1"/>
    <dbReference type="InParanoid" id="G3AJP9"/>
    <dbReference type="OMA" id="PRQWGFF"/>
    <dbReference type="OrthoDB" id="10267115at2759"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047560">
        <property type="term" value="F:3-dehydrosphinganine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006666">
        <property type="term" value="P:3-keto-sphinganine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08939">
        <property type="entry name" value="KDSR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000578">
        <property type="entry name" value="3-ketodihydrosphingosine reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045022">
        <property type="entry name" value="KDSR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43550">
        <property type="entry name" value="3-KETODIHYDROSPHINGOSINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43550:SF3">
        <property type="entry name" value="3-KETODIHYDROSPHINGOSINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00026112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00026241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EGW33950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="304" mass="34111" checksum="7F7B6252D5640F11" modified="2011-11-16" version="1">MWFSKSNFPTAGKTAIIIGASQGIGADLALKLYQKNCSVILVARTESKLIQQITRIKSSIGENENVKLSYYTCDVSNYDNCVDLWQKVVNDGNDPDIIFCCAGSSVPKLFNDLTKQDIQSGITINYNTAINTIHTGFKQLLEKENKHRHVILFSSVVSFYPFIGYSQYAPMKAAIQSLSIVLRQELAPYNYRVSCIFPGNFQSEGYEQEQLTKPEITKKIEGSSHAIPGEECADLIIDQLSKGYDTVTTDFIGWLLGCSVLGVLPRQWGFFQIIVSFLFSIFAPLANWFVNRDIQDSFDKSKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJY5</accession>
    <name>G3AJY5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative zinc finger protein Suc1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_59457</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373620.1">
        <property type="nucleotide sequence ID" value="XM_007373558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJY5"/>
    <dbReference type="FunCoup" id="G3AJY5">
        <property type="interactions" value="635"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJY5"/>
    <dbReference type="GeneID" id="18872924"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59457"/>
    <dbReference type="eggNOG" id="ENOG502S2FW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016574_7_1_1"/>
    <dbReference type="InParanoid" id="G3AJY5"/>
    <dbReference type="OMA" id="SKYWMKS"/>
    <dbReference type="OrthoDB" id="2740448at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050797">
        <property type="entry name" value="Carb_Metab_Trans_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668:SF18">
        <property type="entry name" value="MALTOSE FERMENTATION REGULATORY PROTEIN MAL13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="426"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="486" mass="54527" checksum="F0947FCF52ACA65F" modified="2011-11-16" version="1">MAPPKIHQKTRDTINQISNVNPQDQFIPVEHLLPCLQIYQTFFPGVWPVVSVAQLISVLMNKSYTTMDTLSAYSLSCAISAAVIVQVSFLKNKNHLFPISDTIKSLDFVSQSVKARDMFNYRSHPSLDTLLTSFFLYVYYVNTESGTPAALNYLRESISTAQILGLHNPSTYTNKSPAEVHRMRKIYYLLLVTERFTAIEDCLPVILEPSIPLPSLEDEEYPILISGFRELVKIFAIPDKSFFDRFISLNTNASSLPSSSWIVNVQQQLGNIKIMENAPDIQKLNIIISKYWMKSLTWKLAQKNNLLDEMEPSLSIKFPFAIANDFLDETKDLPISSYESNGPGVCVKLLAMVDTLVDAINTSQDNTGLTSLHGVISLLVQLKNDISLPIHEYNKIINMSNNLSTNAFATVPRNEGYISELGSDDSSERSEHDDINQPSPFTQMTMAFWMGPTGTTPLEVPAPVPVQIQVPFSENSLSDLYNQLYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK06</accession>
    <name>G3AK06_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Large ribosomal subunit protein uL30-like ferredoxin-like fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_59481</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373641.1">
        <property type="nucleotide sequence ID" value="XM_007373579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK06"/>
    <dbReference type="FunCoup" id="G3AK06">
        <property type="interactions" value="380"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK06"/>
    <dbReference type="GeneID" id="18872935"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59481"/>
    <dbReference type="eggNOG" id="KOG3184">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055156_1_0_1"/>
    <dbReference type="InParanoid" id="G3AK06"/>
    <dbReference type="OMA" id="VNGWGPQ"/>
    <dbReference type="OrthoDB" id="28644at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000465">
        <property type="term" value="P:exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF26">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN RLP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="3">
        <location>
            <begin position="102"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="23"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="273" mass="31095" checksum="6885C172869E9696" modified="2011-11-16" version="1">MAILNSNPEILLRKRKNADRKRLEKQEQAREKAVTQKKIKKKQQENKFIRAETLVSNHKSNELEKKRIKNLTKKKQQVLSEEETQGNAKLLFLIRIPNHTKGLKIPTKANQLLTVLKLTEVNTAVFVKATPTVMDMLTLIAPYVIVGQPSLNSVRQLFQKRARILVPDDENPESTKVAKLDTNQLVEDQFGNDLGLICIEDLVHELTSLSDNFVAITSWLLPFKLNPPVNGWGPQAKLAKLLHSTDNKQSISLAQDFKLQEVEDIDKIIEQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKK4</accession>
    <name>G3AKK4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Mannan endo-1,6-alpha-mannosidase</fullName>
            <ecNumber evidence="4 11">3.2.1.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_60943</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>Random hydrolysis of (1-&gt;6)-alpha-D-mannosidic linkages in unbranched (1-&gt;6)-mannans.</text>
            <dbReference type="EC" id="3.2.1.101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glycosyl hydrolase 76 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.101" evidence="4 11"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375124.1">
        <property type="nucleotide sequence ID" value="XM_007375062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKK4"/>
    <dbReference type="STRING" id="619300.G3AKK4"/>
    <dbReference type="GeneID" id="18873650"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60943"/>
    <dbReference type="eggNOG" id="ENOG502QWHG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025694_1_1_1"/>
    <dbReference type="InParanoid" id="G3AKK4"/>
    <dbReference type="OMA" id="GMFQPPY"/>
    <dbReference type="OrthoDB" id="4187847at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008496">
        <property type="term" value="F:mannan endo-1,6-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007117">
        <property type="term" value="P:budding cell bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000006">
        <property type="entry name" value="Mannan endo-1,6-alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005198">
        <property type="entry name" value="Glyco_hydro_76"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014480">
        <property type="entry name" value="Mannan-1_6-alpha_mannosidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145:SF36">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03663">
        <property type="entry name" value="Glyco_hydro_76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016302">
        <property type="entry name" value="Man_a_manosd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442202" description="Mannan endo-1,6-alpha-mannosidase" evidence="13">
        <location>
            <begin position="29"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="436"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR016302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW33609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="458" mass="50458" checksum="B0CB154E0AC5F9CA" modified="2011-11-16" version="1">MVSFKRIPNICITTYISVFLTFTSLVSAVPLTVGDKDSTCAAAKVVADGIWNYYEGLKYGGVVGMFAPPNYWWNAGEAFGGLVDYYTFCDPNNDTLKDLIFNGMYHQAGENYNYVPSNQSMTEGNDDQGVWGMGIMQAAERNFTNPEDHSWISLTQAIFNTMNARWDTSHCNGGLRWQIFTWNSGYNYKNSIANGCLFHLAARLARYTGNSTVYLDVAEKVWDWMDEVGFLTEEDNGNFRIFDGANVETNCTDVTQLRWSYTYGIFMAGCAYLYNFTGDTKWQTRANEIVEASLSYFFQNKIMTETTCAPFNKCNNDQRSFRSLFARCLGLTTLLMPETESVIAPYLEASSAAAAQSCSGGSDGVTCGENWAANGWDGVYGLGEQTSALEVLMSLIVQEPLSVKTGGSNKTDFDAGTKAKDAINKNEIVVTGKDRAGAAVLTAVVLAVILGGAIWMIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKN2</accession>
    <name>G3AKN2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AMMECR1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60970</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375152.1">
        <property type="nucleotide sequence ID" value="XM_007375090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKN2"/>
    <dbReference type="FunCoup" id="G3AKN2">
        <property type="interactions" value="1088"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKN2"/>
    <dbReference type="GeneID" id="18873661"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60970"/>
    <dbReference type="eggNOG" id="KOG3274">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052828_1_0_1"/>
    <dbReference type="InParanoid" id="G3AKN2"/>
    <dbReference type="OMA" id="LFITWNK"/>
    <dbReference type="OrthoDB" id="24630at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.700.20">
        <property type="entry name" value="Hypothetical protein ph0010, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023473">
        <property type="entry name" value="AMMECR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036071">
        <property type="entry name" value="AMMECR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002733">
        <property type="entry name" value="AMMECR1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027485">
        <property type="entry name" value="AMMECR1_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00296">
        <property type="entry name" value="TIGR00296 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016:SF0">
        <property type="entry name" value="AMME SYNDROME CANDIDATE GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016">
        <property type="entry name" value="AMMECR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01871">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143447">
        <property type="entry name" value="AMMECR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51112">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMMECR1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="184" mass="20831" checksum="8AAF0D9B2F399E38" modified="2011-11-16" version="1" fragment="single">MSKALCCYAFETLINKLNIETHKVPLSTYFDALNENLKSLPKSAPLFITWNKNDNLRGCIGTFSDFPLESGVSKFTISAAMHDPRFPAISKSEVNRHLSVSVTLLADFVEIHDQNDWTIGLNGLRISLKYEGEHYSGTFLPSVAEEQGWDKVTTLYHLLKKAEFPLARANVEQFYEKGLKEGWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL50</accession>
    <name>G3AL50_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GTP-binding nuclear protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_60402</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the small GTPase superfamily. Ran family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374607.1">
        <property type="nucleotide sequence ID" value="XM_007374545.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL50"/>
    <dbReference type="FunCoup" id="G3AL50">
        <property type="interactions" value="1558"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL50"/>
    <dbReference type="GeneID" id="18873389"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60402"/>
    <dbReference type="eggNOG" id="KOG0096">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_13_0_1"/>
    <dbReference type="InParanoid" id="G3AL50"/>
    <dbReference type="OMA" id="FNAWDTA"/>
    <dbReference type="OrthoDB" id="48625at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00877">
        <property type="entry name" value="Ran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000131">
        <property type="entry name" value="GTP-binding nuclear protein Ran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002041">
        <property type="entry name" value="Ran_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071:SF0">
        <property type="entry name" value="GTP-BINDING NUCLEAR PROTEIN RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071">
        <property type="entry name" value="RAN GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00627">
        <property type="entry name" value="GTPRANTC4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51418">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="214" mass="24231" checksum="CB8AAA90256EF729" modified="2011-11-16" version="1">MAAEVPTFKLVLVGDGGTGKTTFVKRHLTGEFEKKYIATLGVEVHPLGFHTNFGELKFDVWDTAGQEKFGGLRDGYYINGQCGIIMFDVTSRITYKNVPNWHRDLVRVCENIPIVLCGNKVDVKERKVKAKTITFHRKKNLQYYDISAKSNYNFEKPFLWLARKLVGNAQLEFVASPALAPPEVQVDADLMQKYQQEMEQATALPLPDEDDADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL62</accession>
    <name>G3AL62_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein SYM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60412</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374619.1">
        <property type="nucleotide sequence ID" value="XM_007374557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL62"/>
    <dbReference type="STRING" id="619300.G3AL62"/>
    <dbReference type="GeneID" id="18873394"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60412"/>
    <dbReference type="eggNOG" id="KOG1944">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049109_8_1_1"/>
    <dbReference type="InParanoid" id="G3AL62"/>
    <dbReference type="OMA" id="NLYFIPV"/>
    <dbReference type="OrthoDB" id="430207at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF17">
        <property type="entry name" value="PROTEIN MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="244" mass="28632" checksum="BD13C1AA5B93C578" modified="2011-11-16" version="1">MPTIFHRYNVLLKNYPFRTNMITTGILFGVGDVIAQSLFPHKTSHTEITERGDTREVEVVSEYTFVRTARAVAYGSFFFAPISVLWHGKTLPKIKNPFVSLMRRNRMKEHPQLKKRLHFYDTVFRLTIDQLIFPAFVWIPLYNTVMVTLALHENPLELVRQKLENNWWNVLKASWTVWPGFQLFNLYFIPVHLRIVCSNIWATGWNAFLSFVHNTKGHGKGSGKRIEELLDIEDDEQEITMVYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL65</accession>
    <name>G3AL65_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sphingolipid long chain base-responsive protein PIL1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_60415</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374623.1">
        <property type="nucleotide sequence ID" value="XM_007374561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL65"/>
    <dbReference type="FunCoup" id="G3AL65">
        <property type="interactions" value="454"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL65"/>
    <dbReference type="GeneID" id="18873396"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60415"/>
    <dbReference type="eggNOG" id="ENOG502QQ1T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046464_0_0_1"/>
    <dbReference type="InParanoid" id="G3AL65"/>
    <dbReference type="OMA" id="NRWLGKG"/>
    <dbReference type="OrthoDB" id="5599269at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036286">
        <property type="term" value="C:eisosome filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097446">
        <property type="term" value="P:protein localization to eisosome filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000005">
        <property type="entry name" value="Sphingolipid long chain base-responsive pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028245">
        <property type="entry name" value="PIL1/LSP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962:SF1">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13805">
        <property type="entry name" value="Pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="34966" checksum="34D95F600E70AE04" modified="2011-11-16" version="1">MHRTYSLRSQRAPTAAQLQSPPPPPSSTKSKFFGKGSITHSFRKQAAGALGPELARKLAILIKMEKNLMRSIEITSRERKDVAKQLSLWGETNDDDISDITDKLGVLIYEVGELEDQFIDRYDQYRITLKSIRDIEGSVQPSRERKQKITDQIAYLKYKDPQSPKINVLEQELVRAEAESLVAEAQLSNITREKLKTAFNYQFDSIREHSEKIALIAGYGKALLELLDESPVTPGETRPAYDGYEASKQIIIDAENALASWTFDSAVVRPTLSLAANEEEEGYEDEDLANEAENLRVTEEEWKEAEGQIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALN6</accession>
    <name>G3ALN6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Enhancer of mRNA-decapping protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_60614</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the EDC family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374794.1">
        <property type="nucleotide sequence ID" value="XM_007374732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALN6"/>
    <dbReference type="STRING" id="619300.G3ALN6"/>
    <dbReference type="GeneID" id="18873491"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60614"/>
    <dbReference type="eggNOG" id="ENOG502RIVV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_860511_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALN6"/>
    <dbReference type="OMA" id="GYINYQY"/>
    <dbReference type="OrthoDB" id="4026794at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028322">
        <property type="entry name" value="PNRC-like_rgn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15365">
        <property type="entry name" value="PNRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="261"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="122"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="228"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00061292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="35521" checksum="FBBCA2890C977355" modified="2011-11-16" version="1">MKLAASLSLPSGEKPDFMNEKPKKESRKKKSGTPSNEASLPNGEKPNFQGSRPNKKNIVPEQSLPNGEKPNFQPSKSKKSSHKEQSFPSGEKSNFQHSKSGSKSNFPQEQSLPSGEKPNFFNEKPTSKKNSSKNVKDKKVEDTYAGSSFHSSPLALNLPKPSFKASPKQGGADDELPSSSNSSVASSNSPVSTHGIAAGPGVPPPANAAPLTNPVTAYPPGSIPPPHIYQHPTPQQQYPYYPQFVQPGFSYQVTPQGYIQYQYPPYGQHHPQQMPQHPIYPHYQTPPPPHAAPQGIPPASQQQQQQPQQPAGGHKITFNELLSSSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALR6</accession>
    <name>G3ALR6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thymidylate synthase</fullName>
            <ecNumber evidence="4">2.1.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_60649</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate = 7,8-dihydrofolate + dTMP</text>
            <dbReference type="Rhea" id="RHEA:12104"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="2.1.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thymidylate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.45" evidence="4"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374824.1">
        <property type="nucleotide sequence ID" value="XM_007374762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALR6"/>
    <dbReference type="FunCoup" id="G3ALR6">
        <property type="interactions" value="581"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALR6"/>
    <dbReference type="GeneID" id="18873503"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60649"/>
    <dbReference type="eggNOG" id="KOG0673">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021669_0_2_1"/>
    <dbReference type="InParanoid" id="G3ALR6"/>
    <dbReference type="OMA" id="AYGRFWR"/>
    <dbReference type="OrthoDB" id="766at2759"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00351">
        <property type="entry name" value="TS_Pyrimidine_HMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.572.10:FF:000005">
        <property type="entry name" value="Thymidylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00008">
        <property type="entry name" value="Thymidy_synth_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000398">
        <property type="entry name" value="Thymidylate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020940">
        <property type="entry name" value="Thymidylate_synthase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002497">
        <property type="entry name" value="PRK01827.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03284">
        <property type="entry name" value="thym_sym"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548:SF2">
        <property type="entry name" value="THYMIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="THYMIDYLATE SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00108">
        <property type="entry name" value="THYMDSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00091">
        <property type="entry name" value="THYMIDYLATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate synthase/dCMP hydroxymethylase" evidence="11">
        <location>
            <begin position="10"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW33309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="307" mass="35160" checksum="7B7B8B52F04954CC" modified="2011-11-16" version="1">MTVSPNAAEQAYLDLCFKIINEGEHRPDRTGTGTKSLFAPPQLRFDLSNDTFPLLTTKRVFSKGIIHELLWFISGSTDAKLLSEKGVKIWEGNGSREFLDKLGLTHRREGDLGPVYGFQWRHFGAEYQDCDTDYTGQGFDQLQDVIKKLKTNPYDRRIIMSAWNPPDFKKMALPPCHVFCQFYVNFPENNDGSAKPKLSCLLYQRSCDMGLGVPFNIASYALLTKMIAHIVDMDCGEFIHTLGDAHVYLDHIEALQEQFTRTPRDFPKLVIKEERKSEIKSIDDFKFEDFEIIGYNPHPSIKMNMSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALS4</accession>
    <name>G3ALS4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">eRF1/Pelota-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_60656</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic release factor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374832.1">
        <property type="nucleotide sequence ID" value="XM_007374770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALS4"/>
    <dbReference type="FunCoup" id="G3ALS4">
        <property type="interactions" value="1354"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALS4"/>
    <dbReference type="GeneID" id="18873509"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60656"/>
    <dbReference type="eggNOG" id="KOG0688">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035759_2_1_1"/>
    <dbReference type="InParanoid" id="G3ALS4"/>
    <dbReference type="OMA" id="GPGTEKM"/>
    <dbReference type="OrthoDB" id="10254527at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018444">
        <property type="term" value="C:translation release factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030695">
        <property type="term" value="F:GTPase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002184">
        <property type="term" value="P:cytoplasmic translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009302">
        <property type="term" value="P:sno(s)RNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000001">
        <property type="entry name" value="Eukaryotic peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.960.10:FF:000001">
        <property type="entry name" value="Eukaryotic peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000006">
        <property type="entry name" value="Peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.960.10">
        <property type="entry name" value="eRF1 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024049">
        <property type="entry name" value="eRF1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004403">
        <property type="entry name" value="Peptide_chain-rel_eRF1/aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03676">
        <property type="entry name" value="aRF1_eRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10113">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55481">
        <property type="entry name" value="N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="5">
        <location>
            <begin position="5"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW33317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="438" mass="49387" checksum="20BA31D54B9F5181" modified="2011-11-16" version="1">MSSQETDAEKNIEIWKIKKLIKSLEMARGNGTSMISLVLPPKAQISLTQKMLTDEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNSVPKNGLVVYCGDVITDEGKEKKLNIDFEPFKPINTSLYLCDNKFHVEALNELLENDDRFGFIIMDGNGALFGAVSGNTREVLHKFTVDLPKKHGRGGQSAVRFSRLREEKRHNYVRKVAEVAVQNFITADKVNVKGLILAGSADFKTELSKSDLFDNRLQAKIIKIVDISYGGENGFNQAIELSAETLANVKFVQEKKLLNQYFDEISQDTGKFCYGIEDTLKALDLGACETVIVYENLNTVRYTMKDIEGEEVVANVNPELPDKSWQLDKKTGTEMEIIKEESFLEWLAENYKNYGAELEFVTDRSSEGAQFCQGFGGIGAMLRYKVNFEQLADESDEDEYYDDDDDDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM17</accession>
    <name>G3AM17_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_55254</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SHQ1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374885.1">
        <property type="nucleotide sequence ID" value="XM_007374823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM17"/>
    <dbReference type="FunCoup" id="G3AM17">
        <property type="interactions" value="791"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM17"/>
    <dbReference type="GeneID" id="18871922"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55254"/>
    <dbReference type="eggNOG" id="KOG3247">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030217_1_0_1"/>
    <dbReference type="InParanoid" id="G3AM17"/>
    <dbReference type="OMA" id="HNIESAW"/>
    <dbReference type="OrthoDB" id="73639at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000493">
        <property type="term" value="P:box H/ACA snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000064">
        <property type="entry name" value="Protein SHQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039742">
        <property type="entry name" value="Shq1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048696">
        <property type="entry name" value="SHQ1-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007009">
        <property type="entry name" value="Shq1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12967">
        <property type="entry name" value="PROTEIN SHQ1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12967:SF0">
        <property type="entry name" value="PROTEIN SHQ1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04925">
        <property type="entry name" value="SHQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21413">
        <property type="entry name" value="SHQ1-like_CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="2">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="492" mass="57536" checksum="663B181485D776AF" modified="2011-11-16" version="1">MITPFFEVTQDDEFIYINVKISHIRFSATQIEMIVDNELFIFSLPPYYLRIRLPFPCVDDERSKAEYDSKEEVVRIRIPKEERGRVFPDLDLTSKLLARLNENTSSKAEVSGPLIEELDIDNTMDGKPRDVVQEGEAFDWEIKQEIPNNQELSGVKYGFNNQYDKLVGISVTNGNDINEVGDPETTTASDRVIERLIKENIKFDAEIYAADYIMEKYPTADDDKSFQDLLEWKNPLIRQFLKWYKTQGSIPESERTNVMDVEFTKDEQEKMQNLPRRSYLIDASYNQEILILVICLLFAYHFELRETEGDHNIESAWTIGKLVPQFAFLDSKLVSNPDNSDNVLRAAVITCIRRALAYPFHRSFKLVMKVWEDVYYNLRGGKRLILKALLDLKELFRFHDVYYVYDKIWLEDLVAYLISDNISESVIRKLAYDLKKEYCKVSKTDITFEKMGLDKQDGEDIEEDAEIIALNLQEIELIAEESYQVYRQQTNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3AME6</accession>
    <name>G3AME6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_60144</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EGW32798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_007374251.1"/>
    </dbReference>
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    <dbReference type="InParanoid" id="G3AME6"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="63" mass="7303" checksum="BDD299A18D584CE5" modified="2011-11-16" version="1">MYKLISRLAISISVALTTPYYERIYLHVLSVVTTNKRKRNFSQLHCPLMHSPVTFLYENSSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMK1</accession>
    <name>G3AMK1_SPAPN</name>
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        <recommendedName>
            <fullName evidence="2">Skg3/CAF120-like PH-like domain-containing protein</fullName>
        </recommendedName>
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    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_55323</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374960.1">
        <property type="nucleotide sequence ID" value="XM_007374898.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMK1"/>
    <dbReference type="FunCoup" id="G3AMK1">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMK1"/>
    <dbReference type="GeneID" id="18871927"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55323"/>
    <dbReference type="eggNOG" id="ENOG502RYUU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017094_2_0_1"/>
    <dbReference type="InParanoid" id="G3AMK1"/>
    <dbReference type="OMA" id="IMERTRV"/>
    <dbReference type="OrthoDB" id="5563754at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058155">
        <property type="entry name" value="Skg3/CAF120-like_PH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25381">
        <property type="entry name" value="PH_26"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Skg3/CAF120-like PH-like" evidence="2">
        <location>
            <begin position="192"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Skg3/CAF120-like PH-like" evidence="2">
        <location>
            <begin position="392"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="307"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="357"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="516" mass="58402" checksum="9B052932F8A2C461" modified="2011-11-16" version="1">MIKILNSNGTTPSGASKRTVSDKVKASPSFVRSSRIFEDGLNSSSPSLSGKTLLLENKAQIEALPKEFHPVLYLINAQKLRTYSTGTLEVLAEGSWVDVDARLIGNELTMWRNTESEEFKYINLIDYQLEVVNKLQIKLYQDFVEDSQVYIRFESQVEFNQWLSAIILSKFEYVKLNEAFTAVVLSSKASKLSDTHILMSKKRYSKSEFCNIRLPQISSKWLKVYMVIVPSDNKHLGRIEIYQDDKKVNKKHLIAYIANANHVFNVYPELPAMVDFNSIMNLKGEIHINKHFEHLFAHNNSIPIPKSDSSSSLLASPKMSHSRSTSVNSTSSFFGSPGTSPKPSSMMTFANRDRSSSNESKQSSKASEIVVEKELKEPKRTPSTFLKKHSNDFIMTDSIYIMPISHPGVSPIETMIRNFIPIIDSFKLYGRPQMLISDRKDANCLLFGLPSLPRYEYLSREHVEEAVAAHAGQEFDYEKIISDKIQQLQAKGYRGDGDISNLYNSLNSTSYDLAIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMN8</accession>
    <name>G3AMN8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_60830</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374997.1">
        <property type="nucleotide sequence ID" value="XM_007374935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMN8"/>
    <dbReference type="FunCoup" id="G3AMN8">
        <property type="interactions" value="216"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMN8"/>
    <dbReference type="GeneID" id="18873593"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60830"/>
    <dbReference type="eggNOG" id="KOG1051">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005070_9_1_1"/>
    <dbReference type="InParanoid" id="G3AMN8"/>
    <dbReference type="OMA" id="SHQYISE"/>
    <dbReference type="OrthoDB" id="47330at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19499">
        <property type="entry name" value="RecA-like_ClpB_Hsp104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000120">
        <property type="entry name" value="ATP-dependent chaperone ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000025">
        <property type="entry name" value="ATP-dependent Clp protease subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050130">
        <property type="entry name" value="ClpA_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638:SF176">
        <property type="entry name" value="HEAT SHOCK PROTEIN 78, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="263"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Clp ATPase C-terminal" evidence="8">
        <location>
            <begin position="436"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="76"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="543" mass="61822" checksum="D7A151029AEB74AD" modified="2011-11-16" version="1">MINEPSVEDTISILRGLKERYEVHHGVRIADAALVTAALYSNRYITDRFLPDKAIDLVDEACSTLRLQHESRPDAIASLDRQIMTIEIELESLRKEEDQLSVDRRKKLEADLTMKRDESKKLTDQWESEKRVIDEIKDAKSRLEQAKYELEQSQREGNYGRASELQYATIPELQSKLIELTESEKATQAQALIHDSVTSEDIAAVVSKMTGIPLNNLMKGEKDKLLDMEIILKQKVVGQDEAIHSVADAVRLQRAGLTSDRRPIASFMFMGPTGTGKTELTKALAEFLFNDKSSVIRFDMSEFQERHTVSRLIGSPPGYVGYEDSGELTEAVRRKPYSVVLFDEFEKAHADVSKLLLQVLDEGKLTDSHGKHVDFRNTIIVMTSNIGQEILLNDPNTYEDGHINPDTKKLVLETLKHHYAPEFLNRLDDVLVFNRLSKAALKDILDIRLREISDRLIDKRIILELSQEAKDLLCDLGYEPTYGARPLNRVLQKKLLNPLAMLMIKGQIRERETVHVVAKDREIIVSPNHTEEAAEREIEESNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMT0</accession>
    <name>G3AMT0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_50397</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375039.1">
        <property type="nucleotide sequence ID" value="XM_007374977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMT0"/>
    <dbReference type="FunCoup" id="G3AMT0">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMT0"/>
    <dbReference type="GeneID" id="18871437"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50397"/>
    <dbReference type="eggNOG" id="KOG2043">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002149_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMT0"/>
    <dbReference type="OMA" id="HEWITNT"/>
    <dbReference type="OrthoDB" id="342264at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035361">
        <property type="term" value="C:Cul8-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990683">
        <property type="term" value="P:DNA double-strand break attachment to nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053036">
        <property type="entry name" value="CellCycle_DNARepair_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031906">
        <property type="entry name" value="RTT107_BRCT_6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47667">
        <property type="entry name" value="REGULATOR OF TY1 TRANSPOSITION PROTEIN 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47667:SF1">
        <property type="entry name" value="REGULATOR OF TY1 TRANSPOSITION PROTEIN 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12738">
        <property type="entry name" value="PTCB-BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16770">
        <property type="entry name" value="RTT107_BRCT_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16771">
        <property type="entry name" value="RTT107_BRCT_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="99"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="321"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="443"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="445"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="499"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="813" mass="91643" checksum="B5FF6314521E40BE" modified="2011-11-16" version="1">MFSESSFLIVKSEKLADAEGISKILNENNASAVFIKDDFDNKVNYLSPNPQITHIITESVDFVEYEQAIQSMIPITTSQWVHDSLAQSKIQNIKSYNPDPKYFFHDCFICCADNLPEGDKEIIYGTASAFGAGYLDVLTKYTTHLIAVDLTNEKSIVANSVINSKIKIVVPEWIDHCITLGRKVDENDYLLPNPKILQSDAVFPTATTVSSNDPATNGLEHKSTFLKGKKIYLSQDYNLSKRQNTAICKYLESHEAIICEKFNPDIDIYLGKYRHGEHYIQSCKNPNIVVGTLAYLYNLIITNKWILPTSSQLLHYPLPMNPIPQFTNLKISISNYSGDSRAYLARLISIMGGTFTKTLTRENDYLVCSKPEGKKYLTAKEKWVDETGKPIVKIVNHLWVEECFAQWELQDSNQEKYNHFPKDSLMEASVGNTKLNSEVLKSWYADDDDDDEESKDNEGNVDDSMSEDQSTKRKTKETEQVPDANASTDSKEELMDNSEPASPSTTQTSAKSSSPQVIAEPHHTPVGSRRSAAKRAAAKLHEDMSDLNQYQQMTKSSRKMKEYMTELEHLSSSPNKLLSSPPVKKHKPNKKCEIIAVMTGCESIIDLSADDIANLQNVGVEIISDLSTQPNTLIAPKILRTEKFLRSLAKVDHIVHPKYLIDVLKNVDKQTVLTEFNIEDYRLDKVTKLDQELGKNGLNNLLTSPNKGKLFQDLSFNMSSNLNGGVDVIARILHEHGMKDFHEIKSATKLAKTVIDTHIESTDQVILLANKTKDTKLIAAFKKQIKNGIALEWDWCVKSIFKMQLQNLDDFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMY3</accession>
    <name>G3AMY3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Alpha-1,3-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_55855</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375673.1">
        <property type="nucleotide sequence ID" value="XM_007375611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMY3"/>
    <dbReference type="FunCoup" id="G3AMY3">
        <property type="interactions" value="60"/>
    </dbReference>
    <dbReference type="GeneID" id="18871980"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55855"/>
    <dbReference type="eggNOG" id="ENOG502RZ48">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015387_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMY3"/>
    <dbReference type="OMA" id="GHINGED"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000033">
        <property type="term" value="F:alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392:SF1">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW32397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="781" mass="90548" checksum="44E8EFDC04BD61A8" modified="2011-11-16" version="1">MIEKIHINRSRQKLIIYSIIVIWLLAINLWLFKNSRAKTTLSAFSTAPPHHSSIYNQYDDEVVYKMSQSEIESDLQGLSSTEAGSPVAIKDDFTIQLLQNNLKTKDLRGVDAHAEVYNTIFSNHNIDSIFGNLDFEHRCELYFRNLFLNDHNWAFNPNEDLELIYRDDFAYEDYYRAHSGEIKKKYSEKIDKPEDQINDNDPALKKYIKQEYNSWLNSTRFNEQIVVNGVTTLRIFNKCYISNDNATQVKAMDKFIKNQHNLVSALAASEGERLPSFELTKQESLINLQQSTFEHRVYPWLSFEPPVYESWQGHVYYQPPNLQAIRKGKPQPASKSKSPYFLSQFRDQCNGKGIVLSIGDKHADDTVRLIHLLRALNNKLPIQIVYYDKLSTATKEKIVTAAREPFNSLPRSFAKIAHYFPDDYLTAEENGLPKQDVWFVNTYNVINNEFKDKFKGFGNKFLATLFNSFEEFILVDADTAMMQNPEFFFQLSGYKKSGAFFYKDRSSFEDRKDSDGYFFKKISPSIIDSVMFNIPIITEHTLDREFFRGAFQYMESGLVVLNRKQHYNAMLMMLQLNFYQPVTQRIHGDKEIFWLAMAVSGDERYEFNEYFAAAIGEVTPEADRVRKDGTNAHAKEICSPHPGHINSEDGHSLVWINSGFQFCGQASKIDYDKEYQKNTRLKWLTDANAFKTFYYAPLRISQAVIPPVDIRFRATNTEEEPEKGWHMETEYCNSYMWCAYSSIGGKVEGGGTNTREGTLIEFPQSARDLFAYYGDIWVGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN61</accession>
    <name>G3AN61_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Coatomer subunit epsilon</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_61007</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPI-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the COPE family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375180.1">
        <property type="nucleotide sequence ID" value="XM_007375118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN61"/>
    <dbReference type="STRING" id="619300.G3AN61"/>
    <dbReference type="GeneID" id="18873678"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61007"/>
    <dbReference type="eggNOG" id="KOG3081">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058871_0_0_1"/>
    <dbReference type="InParanoid" id="G3AN61"/>
    <dbReference type="OMA" id="SWVGMRE"/>
    <dbReference type="OrthoDB" id="310217at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006822">
        <property type="entry name" value="Coatomer_esu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10805">
        <property type="entry name" value="COATOMER SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10805:SF0">
        <property type="entry name" value="COATOMER SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04733">
        <property type="entry name" value="Coatomer_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016478">
        <property type="entry name" value="Coatomer_esu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 11" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR016478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="308" mass="35910" checksum="9CAA8757C8F61844" modified="2011-11-16" version="1">MDEFNDSSELYTIRNQFYSNQHNAVISYSLDQFSSQYQLKVLEFQIRSTVALGQDASELIDHGKVLFPEEEELFQLLSAHNDLQSFGTDSSTYFQDVKQGKFELQTVLTAIYLVKYEQDVNQAVSILNEYINSDRYNIGELEPFLILMNLYLYLGKIDQAAKLFEHLKIFPSFIRDSIIYHTFEAWYLAVKGESDNINNSYYFYDELLNLDYENDPIGKQKILTALFALTLQMRHLPEAQELLNQIRQLNVTSGDFIANEICYNYLTGSNDTRELLQQLYQIEPNHQLLQDFAEKTKVFDEIVGKYTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANF1</accession>
    <name>G3ANF1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Adenylyl-sulfate kinase</fullName>
            <ecNumber evidence="4 12">2.7.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_61056</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the synthesis of activated sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24152"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the APS kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.25" evidence="4 12"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375216.1">
        <property type="nucleotide sequence ID" value="XM_007375154.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANF1"/>
    <dbReference type="FunCoup" id="G3ANF1">
        <property type="interactions" value="182"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANF1"/>
    <dbReference type="GeneID" id="18873697"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61056"/>
    <dbReference type="eggNOG" id="KOG0635">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046932_1_0_1"/>
    <dbReference type="InParanoid" id="G3ANF1"/>
    <dbReference type="OMA" id="HENTVEE"/>
    <dbReference type="OrthoDB" id="506431at2759"/>
    <dbReference type="UniPathway" id="UPA00140">
        <property type="reaction ID" value="UER00205"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004020">
        <property type="term" value="F:adenylylsulfate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019344">
        <property type="term" value="P:cysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070814">
        <property type="term" value="P:hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02027">
        <property type="entry name" value="APSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000212">
        <property type="entry name" value="Adenylyl-sulfate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00065">
        <property type="entry name" value="Adenylyl_sulf_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002891">
        <property type="entry name" value="APS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059117">
        <property type="entry name" value="APS_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00455">
        <property type="entry name" value="apsK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003013">
        <property type="entry name" value="PRK03846.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055">
        <property type="entry name" value="BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055:SF1">
        <property type="entry name" value="PAPS SYNTHETASE, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01583">
        <property type="entry name" value="APS_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APS kinase" evidence="13">
        <location>
            <begin position="23"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW31940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="200" mass="22543" checksum="834A8DF6D2B51CBC" modified="2011-11-16" version="1">MATNITWHPNLTHAERAELRKQQGVTVWLTGLSASGKSTIACALEQSILARGLNAYRLDGDNIRFGLNKDLGFSEADRNENIRRISEVSKLFSDSCCVTITSFISPYKADRQVARELHEKDNIPFVEVYVDVPIEVAEQRDPKGLYKKAREGVIKEFTGISAPYEAPEKPEIHLKNHSGVSIEEAAEQIIDYLLEKKYIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANH8</accession>
    <name>G3ANH8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Electron transfer flavoprotein-ubiquinone oxidoreductase</fullName>
            <shortName evidence="19">ETF-QO</shortName>
            <ecNumber evidence="19">1.5.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ETF1</name>
        <name evidence="21" type="ORF">SPAPADRAFT_55543</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 19">Accepts electrons from ETF and reduces ubiquinone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 19">
            <text>a ubiquinone + reduced [electron-transfer flavoprotein] = a ubiquinol + oxidized [electron-transfer flavoprotein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24052"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.5.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="19">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ETF-QO/FixC family.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.1" evidence="19"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375243.1">
        <property type="nucleotide sequence ID" value="XM_007375181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANH8"/>
    <dbReference type="FunCoup" id="G3ANH8">
        <property type="interactions" value="720"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANH8"/>
    <dbReference type="GeneID" id="18871947"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55543"/>
    <dbReference type="eggNOG" id="KOG2415">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009667_4_0_1"/>
    <dbReference type="InParanoid" id="G3ANH8"/>
    <dbReference type="OMA" id="INFQNCV"/>
    <dbReference type="OrthoDB" id="437331at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000015">
        <property type="entry name" value="Electron transfer flavoprotein-ubiquinone oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040156">
        <property type="entry name" value="ETF-QO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049398">
        <property type="entry name" value="ETF-QO/FixC_UQ-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007859">
        <property type="entry name" value="ETF-QO/FixX_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10617">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN-UBIQUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10617:SF107">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN-UBIQUINONE OXIDOREDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21162">
        <property type="entry name" value="ETFQO_UQ-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05187">
        <property type="entry name" value="Fer4_ETF_QO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9 19" id="KW-0274">FAD</keyword>
    <keyword evidence="6 19" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13 19" id="KW-0408">Iron</keyword>
    <keyword evidence="14 19" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 19" id="KW-0813">Transport</keyword>
    <keyword evidence="15 19 21" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="20">
        <location>
            <begin position="577"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00052682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU366068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EGW31967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="617" mass="68294" checksum="CEBC745394FE9839" modified="2011-11-16" version="1">MASRAILIRSTRLLGRSSYSALRTPWRFPTRSFTTEQLEILNQERPSDHVDVCIVGGGPAGLATAIKLKQLDNEKGSGELRVVVLEKAGDFGGHTVSGAILEPGAFLELFPDAQDLVEESGGIPLPKDIVTKVTKDGMKYLTEKHTFPLPEVPALANHGKNFVVSLNNVVKYLSEQAEELGVELYPGISVSELIYNESGDAIKGVATQDMGIAKDGTPKDSFERGMEFLARVTVLAEGCHGSLTKKAISKFDLRKDCLPQTYGLGIKEVWEVKPENFKPGFVAHTMGYPLSFQEYGGGFQYHFGDGLVAVGLVIGLDYANPYVSPYQEFQKLKLHPYYSKVLEGGKCISYAARALNEGGYQSIPQLHFPGGLLVGCSAGFVNVPKIKGTHTAVKSGIVAAEEIFKIYQEQEFEPVDEDTFDDEYKALDLSSYEESFKQSWAYKELYAVRNVRPSFNTPLRILGGLTHSGLTTLITKGYEPWTLKHKHTDSSMTQDASKFKPIDYPKPDGVLTFDILTSVSRTGTSHDEDEPCHLKIPNQDERKHAEISWPKYKGVEQRFCPAGVYEYIEDANEPLGVKFQINSQNCIHCKTCDIKVPTQDIDWEVPEGGDGPKYYMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANL9</accession>
    <name>G3ANL9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Eukaryotic translation initiation factor 3 subunit A</fullName>
            <shortName evidence="7">eIF3a</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Eukaryotic translation initiation factor 3 110 kDa subunit homolog</fullName>
            <shortName evidence="7">eIF3 p110</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Translation initiation factor eIF3, p110 subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RPG1</name>
        <name evidence="7" type="synonym">TIF32</name>
        <name evidence="10" type="ORF">SPAPADRAFT_139247</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the eIF-3 subunit A family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375824.1">
        <property type="nucleotide sequence ID" value="XM_007375762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANL9"/>
    <dbReference type="FunCoup" id="G3ANL9">
        <property type="interactions" value="1315"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANL9"/>
    <dbReference type="GeneID" id="18870193"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139247"/>
    <dbReference type="eggNOG" id="KOG2072">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002096_2_1_1"/>
    <dbReference type="InParanoid" id="G3ANL9"/>
    <dbReference type="OMA" id="EHITNKR"/>
    <dbReference type="OrthoDB" id="18884at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071540">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3e"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043614">
        <property type="term" value="C:multi-eIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.10:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.10">
        <property type="entry name" value="UVR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03000">
        <property type="entry name" value="eIF3a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027512">
        <property type="entry name" value="EIF3A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054711">
        <property type="entry name" value="eIF3a_PCI_TPR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14005:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14005">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3, THETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22591">
        <property type="entry name" value="eIF3a_PCI_TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7 10" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PCI" evidence="9">
        <location>
            <begin position="338"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="862"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="587"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="807"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="881"/>
            <end position="894"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="894" mass="102555" checksum="CCF926FF6CF2AC9C" modified="2011-11-16" version="1">MAPPHKSHAFRPENVLKRAEDLIAVGEHEAALDTLYELITAKRIRYLAVEDLQPIALLLIKLAVDLRKGKLAKDALHQYKKNVQMSENGLESVQVIVTKFIELSEAKLDEAQAKADIKIDQEEHDHDDDLEVASTPETILLSAVSNTDSADRTERELVTPWLRFLWEAFRAVLDILRNNSKLEVTYSAIVNQAFKFCLNYKRKAEFRRLCELLRTHMQSVTGTGAPGATTTATTSSYSTYAIDLSDSETVQRYLEQRFSQLNIAVKLELWQESFRSVDDVHTLLTASKKAPKPTMMANYYENVARIFAVSDNSLFHAAAWNKFFNLYSQSPNATDDELKRYASILVLSTLSIPQKAVHADDEFKTKNSKLSSLLNLNQVPTRDGLIKSIVSRAILRYVDEPIATLFQLLEGGDFHPLSIKDKVTSIFQLIEQDKDYKKYIATLTEVVALRVFQQVSQVYESVKLDFLISLAIFPDLTHSLTPLEVEDLIVNAAKDGQLNLTIDHETDVVSFKQNPFEETYQDYPTGKLQVSPSELIRFQISKLARTLYESVKIVDPNYELAQQQAAESASARALQSMVDEQAALADRVRIMEERKALIEKRKREEEEIAARLKAEKLVAEQKAEQERLLLEAERKKAEKLEKERLRIQLSEKRKVAEEINAKGIIKIDLDKLEELDTDKLKLMQIEQLNKDKRELEEKLSQTAKKVDYLERAFRRAELKHLEEDEIHERQVELDNYNAIKQTKIARAKKEYDESVGLKTRLERIVPDYSDFKKIIDAKNAAKVAQLKQEAAAKFEQAKLERIEEIKKKRIEELKVRKDRERKAAAEEAAKHKKEAEMARFKEELRVQREKDEALAKKRAEIEAQVAKEVAPEPKPAPKMSFAERMRLKREGKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANN9</accession>
    <name>G3ANN9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_51112</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375844.1">
        <property type="nucleotide sequence ID" value="XM_007375782.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="G3ANN9">
        <property type="interactions" value="740"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANN9"/>
    <dbReference type="GeneID" id="18871518"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51112"/>
    <dbReference type="eggNOG" id="KOG0671">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_5_8_1"/>
    <dbReference type="InParanoid" id="G3ANN9"/>
    <dbReference type="OMA" id="YKNHICL"/>
    <dbReference type="OrthoDB" id="283111at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016480">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14134">
        <property type="entry name" value="PKc_CLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051175">
        <property type="entry name" value="CLK_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="293"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="61"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="573"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="707" mass="80568" checksum="E2BE733777E42DCA" modified="2011-11-16" version="1">MSLITNRKRPRGFSEAFDSATTKPKSTGGVVVSNTTDMDSAAQQAKTIHLNFDPTISDSNLSSNSINTSPPLYHASHVQQQQQQQQHRSFINSDSDTGLNTNLLFGNSYRISPLTRRYSSGAISKPKQHAFTNPFLSAEEAAAAAAEEVFIAEEDEDELEMEDHEQNVDDEDDDDLIILEERDLIPKAKRNNSDPVFDQDALVAYNMFNVNNQAPTFEIGKNNPIKKQRTSSLPQLPQAKCFYNKLPNNYILQTPKLGNIKTNVIPHQSNLPPCDDENGHYIIKINEPFANRFIIIKLLGQGTFGKVVQCFDKVNREHVAIKIIRNIQKYRDAAKIELRILSTLKKFDNKNANHCIHLRECFDYRGHICIVTDLLKISLYDFLENNKFIPFPGSHIQAISKQLIRSVTFLHDLNLIHTDLKPENILLHDDNYSKKNLLTSTIISAYMKLGNSNTKKVPKYSKILKDPLIQVIDFGSAIFNDEYHSSIVSTRHYRAPEIVLGTGWSFPCDMWSIGCILVELIIGEPLFKTHDNLEHLAMIEKVCGYRIDKSMVKLSKKKENECGLEYFTNEFIDDDDRSENSSDDSENSSDDDDDLILDHESTYQNSLVLKFPTSSTPQKFINSVEELSRIDLFISSRIGLAIDFDYSLSANYEKNKGVINFGNFTFWWFFIDLLRKLFIINPEDRIEAIEALNHPWFNLGIEDEGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANX6</accession>
    <name>G3ANX6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_51142</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375877.1">
        <property type="nucleotide sequence ID" value="XM_007375815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANX6"/>
    <dbReference type="GeneID" id="18871524"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51142"/>
    <dbReference type="eggNOG" id="ENOG502SX3Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119129_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANX6"/>
    <dbReference type="OMA" id="PNWKKAP"/>
    <dbReference type="OrthoDB" id="3784821at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="155" mass="17527" checksum="D976B46B83EFABB6" modified="2011-11-16" version="1">MAAEEPQPPRTAKELKSTIKIQPAVGIPGVLAGPKPHVDSPFVQHLITTGKPNWKLATPEMKTKYRAYQKILIGIPVLFICSYVLVRRLNGESTKQMQKGEIAEDGTLIYWDENKIMEVERNTWSYKLFGPDYFQEGRTSRTIGKPLAIKPDDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP18</accession>
    <name>G3AP18_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_138715</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 14 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375325.1">
        <property type="nucleotide sequence ID" value="XM_007375263.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP18"/>
    <dbReference type="FunCoup" id="G3AP18">
        <property type="interactions" value="255"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP18"/>
    <dbReference type="GeneID" id="18870143"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138715"/>
    <dbReference type="eggNOG" id="KOG1875">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_283151_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP18"/>
    <dbReference type="OMA" id="FIKIYTL"/>
    <dbReference type="OrthoDB" id="205099at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055122">
        <property type="entry name" value="Med14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013947">
        <property type="entry name" value="Mediator_Med14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12809">
        <property type="entry name" value="MEDIATOR COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12809:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08638">
        <property type="entry name" value="Med14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit MED14 N-terminal" evidence="10">
        <location>
            <begin position="24"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="987" mass="114318" checksum="708A63E72ED49823" modified="2011-11-16" version="1">MNEDINGQDTSKVPPLPHVTTNILPLSSIIHFHTQEAYKQFTTAIDNLAADQSNDVKRKKQFLELIISLRHDFVKLYTLVKWCSNSADVSKLIDLLNWLRQLEFSYDQLLYTMNTEFGNSQFNYGTKLPNSDLITSLQVLYKQRPQLASYGYLRTDNDVVSSEKILEVLKELNLVLTIRFALMNDVELPPRFNNNYEIKDGKCFITIPNEYQLVITTISQTSPFYLIDFKFLFGINQETLSISHNSYTKLPPKSYKKLEKIVNTVFNTSTSTSLTEVYELLHKYSMSFKLYLIAKQIKELMVNSKWRNNIDLNYSIGRNSLILNYWSSHYLSKNYKSFIELGIDRNYNLNYRWFKNGQYQQTDMTEIFIQSQPEDNEEVIIDDDDDEGNDDHPHDLNVDVMLNVIVNKHAELLMSSVFERLAGLLSPEEISQVNSHQLLVKLSHNKSTIFAINPLTGYFYFIDPITIQQAITKKINTAPSTSSKFISEDDLINNIIDQIIQLRLLVYTKEINNKLLTSQWINNDIITLSETELSKLGNSGKCQFYRVKKWPSSWFLINSINGINNSVAWYVARIKSIKGEWKIQWKNKISHDSELNYGFFSGLATICSNMIIDHMIIEELQTKHIKYFKVDADKERKVLAKFGIKEKPSVEGTIYQSLIMIYNDELLPINNSSTSLFLQIQLLSDHKMNLTLFGNLRNLSIIQEDYSQLNLTIIEENFEFKDTVNLSTATMTERNLLSKLFNNLNQFNKLIKILDQLNNNHIQIVSNTINDITINIDGNNLTILLPEESTMCIQLTTTTENSQLRLILEYLNRYLHTTNTSSVIGIIKYLQTINPILNAITTINKLVNKQTFRLSNGLSRLNFDVTLNTLNSIQFIFHISSSTNSKKIIKDKITVNISFKNNKFDKQKRNLVKISLRDNLIDKNLKFKKLFELIFKSINDLEQTKKNDTSVALFKLNYDFLISGNILEELLERIGNSFISYLQLEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP93</accession>
    <name>G3AP93_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-dehydropantolactone reductase</fullName>
            <ecNumber evidence="6">1.1.1.358</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2-dehydropantolactone reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Ketopantoyl-lactone reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_138169</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(R)-pantolactone + NADP(+) = 2-dehydropantolactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16719"/>
            <dbReference type="ChEBI" id="CHEBI:18395"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.358"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>isatin + NADPH + H(+) = 3-hydroxyindolin-2-one + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:68608"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27539"/>
            <dbReference type="ChEBI" id="CHEBI:28536"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.358" evidence="6"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375940.1">
        <property type="nucleotide sequence ID" value="XM_007375878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP93"/>
    <dbReference type="STRING" id="619300.G3AP93"/>
    <dbReference type="GeneID" id="18870101"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138169"/>
    <dbReference type="eggNOG" id="KOG1577">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_0_3_1"/>
    <dbReference type="InParanoid" id="G3AP93"/>
    <dbReference type="OMA" id="YSHHPEY"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047011">
        <property type="term" value="F:2-dehydropantolactone reductase (A-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042180">
        <property type="term" value="P:ketone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF3">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="12">
        <location>
            <begin position="16"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="11">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00079693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00081322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW32664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="306" mass="35091" checksum="982D0C832E6A4021" modified="2011-11-16" version="1">MITELKTVSGDPITVGFGTGTVVKHLKDPHDHTATSDEIVKLLTYAISIGYNHIDTAAVYYTQPEVGKAFYESGIEREKVFLSTKFSVFKDEEEKSTITATEFVEKTLKELRTDYIDLLLIHHPFFEPDHSESKYTLHSLWSEFIKIKQSGKVRYIGVSNFSISHLSSVIAVSKPFGREYFPVVNQIEFHACLQNQSPDIVSFCQINGILVHGYSPLTPLFRLQEGNVYLHRLLMDLETKYKKTRAQILLRYCLDKKIVPITTSCQETRLKECIDVYDFNLEEQDIKKIDKEGAKSPHQTYFAGKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3APE0</accession>
    <name>G3APE0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CAF1B/HIR1 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_61202</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat HIR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375393.1">
        <property type="nucleotide sequence ID" value="XM_007375331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APE0"/>
    <dbReference type="STRING" id="619300.G3APE0"/>
    <dbReference type="GeneID" id="18873773"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61202"/>
    <dbReference type="eggNOG" id="KOG1009">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010127_1_2_1"/>
    <dbReference type="InParanoid" id="G3APE0"/>
    <dbReference type="OMA" id="MHIFAFI"/>
    <dbReference type="OrthoDB" id="71227at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033186">
        <property type="term" value="C:CAF-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055410">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045145">
        <property type="entry name" value="PTHR15271"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15271">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15271:SF4">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24105">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CAF1B/HIR1 beta-propeller" evidence="10">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="10"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="64"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="126"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="176"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="216" mass="24037" checksum="FD479D5E08708B06" modified="2011-11-16" version="1" fragment="single">MEASTITVHWHNDNQPIYAIDFQPGSTPRLVTGGGDNNIRMWNLTTNSGSGEVNNQSVEYLSTLRKHTQAVNLVRFSPDGTLLASAGDDGTLMLWRLCDGIVKDFGADEDEDDDDIKESWKVVAQFRSGTSEIMDVCWSPCGKYLVSGSMDNTVRVYQLSIGNDADKVTGKLIQSLKSHSHYIQGVYWDPLDEYIVSQSADRSVNVYKIIRKHSAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3APV4</accession>
    <name>G3APV4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61362</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375551.1">
        <property type="nucleotide sequence ID" value="XM_007375489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APV4"/>
    <dbReference type="GeneID" id="18873858"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61362"/>
    <dbReference type="eggNOG" id="ENOG502RMMP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113788_0_0_1"/>
    <dbReference type="InParanoid" id="G3APV4"/>
    <dbReference type="OMA" id="IYATEYI"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442532" evidence="2">
        <location>
            <begin position="20"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="151" mass="16099" checksum="D259EE1718923FDC" modified="2011-11-16" version="1">MQFSILSLTLLSLFKLATADDYDATTVVTTTPHTTEVTTFVAQEDIYGTVYIYTNDDGQLTTQTVLNTQTNSYDPATTTTSSSSSSASSSTTASSYSLYNDPVSETDTTTSEPTLVIPVGDYTTSTKISTTTLDNGNTAIVELVVLYTSDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ52</accession>
    <name>G3AQ52_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein MSS4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">MSS4</name>
        <name evidence="3" type="ORF">SPAPADRAFT_61964</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376177.1">
        <property type="nucleotide sequence ID" value="XM_007376115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ52"/>
    <dbReference type="STRING" id="619300.G3AQ52"/>
    <dbReference type="GeneID" id="18874126"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61964"/>
    <dbReference type="eggNOG" id="KOG0229">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004312_6_0_1"/>
    <dbReference type="InParanoid" id="G3AQ52"/>
    <dbReference type="OMA" id="NSNMKER"/>
    <dbReference type="OrthoDB" id="20783at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF8">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 5-PHOSPHATE 4-KINASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="2">
        <location>
            <begin position="1"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="305" mass="35453" checksum="89D818B108E2D666" modified="2011-11-16" version="1">MSITGKYILSQLSSPGKSGSFFYYSRDYRFIIKTIHHAEHRQLRRILKDYHSHVKENPNTLVSQIYGLHRIKMPLGSGRRVVHFVVMNNLFPPHRDIHLKYDLKGSTHGRITRINAHETDLSKYTLKDLNFLERHEMIKFGPAKRQVFFKQLEADVKFLKKVNVMDYSLLMGIHYVNQGNKNGINLSIFDPKSGDKTVLINTNPRDIERQDLPTDVYPGRSKYVFYGHDGGIRATDEENKPLAEIYYMGIIDCLTGYRTKKSLETFFRSMWNSRSVISAVPPREYGDRFLDFIKKGTGQAKKKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ70</accession>
    <name>G3AQ70_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosomal protein L35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_56272</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376195.1">
        <property type="nucleotide sequence ID" value="XM_007376133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ70"/>
    <dbReference type="FunCoup" id="G3AQ70">
        <property type="interactions" value="927"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ70"/>
    <dbReference type="GeneID" id="18872022"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56272"/>
    <dbReference type="eggNOG" id="KOG3436">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110381_1_1_1"/>
    <dbReference type="InParanoid" id="G3AQ70"/>
    <dbReference type="OMA" id="VMNQKAR"/>
    <dbReference type="OrthoDB" id="528635at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00427">
        <property type="entry name" value="Ribosomal_L29_HIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.310:FF:000002">
        <property type="entry name" value="60S ribosomal protein L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3450:FF:000001">
        <property type="entry name" value="60S ribosomal protein L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_uL29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_uL29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045059">
        <property type="entry name" value="Ribosomal_uL29_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_uL29_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL29-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="Ribosomal protein L29 (L29p)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW31417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="14142" checksum="2059B4A78BB61423" modified="2011-11-16" version="1">MAAVKTFELRTKSKEQLEQQLVELKQELATLKVQKLQRPSLPKIHTVRKNIARVLTVINLTQRENVKAFYAGKKYQPKDLRAKKTRAIRRRLTKFEANKETEKARKQRIAFPQRKFAIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ71</accession>
    <name>G3AQ71_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_51435</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ubiquitin conjugation factor E4 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376196.1">
        <property type="nucleotide sequence ID" value="XM_007376134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ71"/>
    <dbReference type="FunCoup" id="G3AQ71">
        <property type="interactions" value="1280"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ71"/>
    <dbReference type="GeneID" id="18871556"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51435"/>
    <dbReference type="eggNOG" id="KOG2042">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003224_0_1_1"/>
    <dbReference type="InParanoid" id="G3AQ71"/>
    <dbReference type="OMA" id="SNAFMTN"/>
    <dbReference type="OrthoDB" id="20295at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034450">
        <property type="term" value="F:ubiquitin-ubiquitin ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071361">
        <property type="term" value="P:cellular response to ethanol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16657">
        <property type="entry name" value="RING-Ubox_UBE4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000055">
        <property type="entry name" value="Ubiquitin conjugation factor e4 a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019474">
        <property type="entry name" value="Ub_conjug_fac_E4_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045132">
        <property type="entry name" value="UBE4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931:SF2">
        <property type="entry name" value="UBIQUITIN CONJUGATION FACTOR E4 B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931">
        <property type="entry name" value="UBIQUITINATION FACTOR E4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10408">
        <property type="entry name" value="Ufd2P_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="12">
        <location>
            <begin position="974"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="40"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1050" mass="121368" checksum="333A0B0506F8616F" modified="2011-11-16" version="1">MASQDEIRAKRLAKLQQHEAQQAQKKQQTEQELAQQLQQAQQELKVEQGKAQPKEHVPPVVSAEKELPVVEQELSDHTIADTTATDPFTEWVLTQLEFIFQATIDPEKNKGDLVFLYNLAYELQQRPHGQITPDDIESIFMEILTEVGIPAKSKSLMEYLYLTYHNAFRIKRTIPLKAHLYQEKVQFLNQVIKFSVSYGGISFHVVDLFINNDVSKSLEFFVTRFNDVSSFLFDIINNEEDEASLLELANLIFPYLSFKISTANLGHSSNFIHLLSVYEALVSIKPVAAIFSQVNGFQPPKQECLDYEHKTLLGPLLRISPLLEDMAVNYFQGVSTQTPMQIHNTYSSIQNEYNVIISHLFTIVDKLVRGSVKTRTDLVEWFADLINLNHLRRGSHADFKKLSSDGIMYNISIILIKLSLPFLNYPLFGKIDKIDVDYFRKNRLLDVDEESRVNSTIEEVEEQYSHEEQDTNFISDCFNLTLAYLHYGIGGIFIKYDRLKQQIKQVQSRIDQIESGQTPPGLNPMMISFLRQELPKLKKTIEAFKSVKHSIQAIFDFRNLQLEIFDFIIGATTFIIRLIDPSHSYPQSKLSIPIFKISKVSELDDHDYLKTKTPIPWKYYPEFIVEGIVNYCKFSTNFMGCPLVDNQDKLTCFCEFIIILLRCPELVGNPHLKSNLVEILFVGSLPIENANRDGFMISVFNNNKLIIDNILYSLLDFYVMVEKTGASSQFYDKFNSRYYISVILEQLWKNPSYRFQLKDYSRNNVDFFIRFIARMLNDTTYLLDETFNVLNEIHNVQQELKKRLTSPGDESMGTDEELEGNLDSLESRAKSYMSLSNKTMELFKLFTKEVPQGFMLAEIIDRLASMLNYNLSIMVGPKCSNLKVENPEKYNFEPKKILTLLCEIYVNLSSQKEFVASVARDERSFNLAYFEKAENILTTKTYVEPKTINALVTFARAAEDQRKLEEMEEMEMGEIPDEFLDPLMFTLMKDPVILPGSRVSIDRSTIKAHLLSDSTDPFNRMPLTLDDVIDNVELKQQIDEFRQKARQNRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ92</accession>
    <name>G3AQ92_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Alpha-1,3-glucosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_56290</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate = an alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31307"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="Rhea" id="RHEA-COMP:19521"/>
            <dbReference type="Rhea" id="RHEA-COMP:19522"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:132521"/>
            <dbReference type="ChEBI" id="CHEBI:132522"/>
            <dbReference type="EC" id="2.4.1.265"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:31308"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ALG6/ALG8 glucosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376217.1">
        <property type="nucleotide sequence ID" value="XM_007376155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ92"/>
    <dbReference type="FunCoup" id="G3AQ92">
        <property type="interactions" value="789"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ92"/>
    <dbReference type="GeneID" id="18872024"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56290"/>
    <dbReference type="eggNOG" id="KOG2576">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022045_1_1_1"/>
    <dbReference type="InParanoid" id="G3AQ92"/>
    <dbReference type="OMA" id="YHSTDFD"/>
    <dbReference type="OrthoDB" id="1689333at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042283">
        <property type="term" value="F:dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004856">
        <property type="entry name" value="Glyco_trans_ALG6/ALG8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413">
        <property type="entry name" value="DOLICHYL GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413:SF2">
        <property type="entry name" value="DOLICHYL PYROPHOSPHATE GLC1MAN9GLCNAC2 ALPHA-1,3-GLUCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03155">
        <property type="entry name" value="Alg6_Alg8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="462"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="492"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="539"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="573" mass="66630" checksum="C3A3A50A1A551B0A" modified="2011-11-16" version="1">MAKPKVTSVSNLKFAKEPELSTTDPLPERESTYSLKNIWIVSFTLKALLVVGYHSTDFDVHRNWLAITHNLPISKWYVENTSQWTLDYPPFFAYFEWFLSQMVPDFVKRDGCLDIVEKGQYGLPTVYFQRLTVIVSELVLFYALQWMIDSSPNFPAKRRTYVAVGSLALSPGLLIIDHMHFQYNGMMYGILLLCLNCARLKRYLMCGFWFSVLLCFKHIYLYLAPAVFVFLLRAYCLKLNYNKKRNVFVNLISFVQWGNLLKLGSVVIAVFTIAFLPFIYYGVGPNLISRLFPFSRGLTHAYWAPNVWAIYSFADRFLIQIYKRIPISRYPLQRLFQFVPELLENDELLKSSTRGIVGDIEFFILPTITPKLTFLLTFFYQIMSLIPLFVQPNYRRFIGALTLCGYASFLFGWHVHEKAILIVIFPMTLLVARDRKLLAPYSLLVACGYGSLFPLIYTSNEWLVKVLYTLLWYIIFYFNLRKVVPIPKKFEAYGGGGIILDRVINGYILGFIVVVTFTSLLDLLETRYTILQNFKFLNLMIYSVYCGVGIISSWNGFYWLYFVDESIWSGDDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQG9</accession>
    <name>G3AQG9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">Ribonuclease P/MRP protein subunit POP5</fullName>
            <ecNumber evidence="4 10">3.1.26.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_140191</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP, which cleaves pre-rRNA sequences.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the eukaryotic/archaeal RNase P protein component 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="4 10"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376294.1">
        <property type="nucleotide sequence ID" value="XM_007376232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQG9"/>
    <dbReference type="FunCoup" id="G3AQG9">
        <property type="interactions" value="284"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQG9"/>
    <dbReference type="GeneID" id="18870274"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140191"/>
    <dbReference type="eggNOG" id="KOG4639">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086710_1_2_1"/>
    <dbReference type="InParanoid" id="G3AQG9"/>
    <dbReference type="OMA" id="IVRCPRA"/>
    <dbReference type="OrthoDB" id="24745at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000171">
        <property type="term" value="F:ribonuclease MRP activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034965">
        <property type="term" value="P:intronic box C/D snoRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000294">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, RNase MRP-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3250:FF:000004">
        <property type="entry name" value="Ribonuclease P/MRP protein subunit POP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3250">
        <property type="entry name" value="Ribonuclease P, Pop5 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00755">
        <property type="entry name" value="RNase_P_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002759">
        <property type="entry name" value="Pop5/Rpp14/Rnp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016819">
        <property type="entry name" value="RNase_P/MRP_POP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038085">
        <property type="entry name" value="Rnp2-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441:SF2">
        <property type="entry name" value="RIBONUCLEASE P_MRP PROTEIN SUBUNIT POP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01900">
        <property type="entry name" value="RNase_P_Rpp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023803">
        <property type="entry name" value="Ribonuclease_P_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160350">
        <property type="entry name" value="Rnp2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR023803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW31516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="173" mass="19505" checksum="9133AFD15020462A" modified="2011-11-16" version="1">MVRVKQRYILFEILYPPAYDPKHSDEDFTNFSSSERNALLSLHQTSSSDINQKSITNAIRKSLQIHYGDFGGGSAGMLMTVKYFSNKTSTGIVRCGRGHFELIVGALALITRIEGYDVIIRCTHVSGTIKKCEEYSIQKSKQLMHLMSQNGSDLTSWMSDVNHMDINEDDEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQJ2</accession>
    <name>G3AQJ2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62134</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376317.1">
        <property type="nucleotide sequence ID" value="XM_007376255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQJ2"/>
    <dbReference type="GeneID" id="18874195"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62134"/>
    <dbReference type="eggNOG" id="ENOG502RJ95">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1907998_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQJ2"/>
    <dbReference type="OMA" id="YECAFCK"/>
    <dbReference type="OrthoDB" id="4077708at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="133" mass="15398" checksum="E879010FDD0EA993" modified="2011-11-16" version="1">MPSTTDNSYYHYTTSKHININDLDMEYDNEEIFELEDTDSISDASTISSINSIHQESPLLSSTYYQQYNSSEENVDLLYECCICKQKMSNDTLHDCLSTISGEKPDIVIQSNNELIQTNYRKWLFNITPNVPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR03</accession>
    <name>G3AR03_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_140433</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SKN1/KRE6 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376442.1">
        <property type="nucleotide sequence ID" value="XM_007376380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR03"/>
    <dbReference type="STRING" id="619300.G3AR03"/>
    <dbReference type="GeneID" id="18870290"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140433"/>
    <dbReference type="eggNOG" id="ENOG502QR13">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010811_4_3_1"/>
    <dbReference type="InParanoid" id="G3AR03"/>
    <dbReference type="OMA" id="TAPFDDY"/>
    <dbReference type="OrthoDB" id="412647at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015926">
        <property type="term" value="F:glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02180">
        <property type="entry name" value="GH16_fungal_KRE6_glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000140">
        <property type="entry name" value="Beta-glucan synthesis-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005629">
        <property type="entry name" value="Skn1/Kre6/Sbg1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31361">
        <property type="entry name" value="BETA-GLUCAN SYNTHESIS-ASSOCIATED PROTEIN KRE6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31361:SF1">
        <property type="entry name" value="BETA-GLUCAN SYNTHESIS-ASSOCIATED PROTEIN KRE6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03935">
        <property type="entry name" value="SKN1_KRE6_Sbg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="10">
        <location>
            <begin position="121"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW31664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="525" mass="59327" checksum="79AC999C6FCA71F8" modified="2011-11-16" version="1">MSYFSKNSDLMSSYEPDYSPFGGYPATAFPLFLHEKEPDDFLHNPDKSDADYDKHRFWHDLRHMDRRSFGGLIGLFFFLGCAVVAFAVFPILTFMQSSVLHKEVVEVLTPYSYPMLSAIRQELVDPDTPKEFRTNVARDGSKWKLVFSDEFNKEGRTFYEGDDQFFTAPDIHYAATNDLEWYDPDAVTTANGTLQLRMDAFNNHDLFYRSGMVQSWNKMCFTQGKLEFSAKLPGSGSVSGLWPGMWSLGNLGRPGFLATTEGVWPYTYSSCDAGITPNQSSSDGISYLPGQRLNSCTCPGESHPNPGVGRGSPEVDALEGEIHGNVGRVSQSLQVAPYDIWYMPDYDFIEIHNSSVTLMNTYAGGPFQEAISGVTMLNTTWYEYGQGEGNYQKYGYELMNHDTNGYITWYVGDDPTYTLYAKALHPNGNIGWRHIPKEPLSIVMNLGISNNWAYINWPALTFPSTMRIDYVRVYQPEDQVNIGCDPPGYPTFDYIEQHLNIYSDANITKFEKGGYKIPKNKLTGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR68</accession>
    <name>G3AR68_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein GAP2.1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">GAP2.1</name>
        <name evidence="11" type="ORF">SPAPADRAFT_56131</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376021.1">
        <property type="nucleotide sequence ID" value="XM_007375959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR68"/>
    <dbReference type="FunCoup" id="G3AR68">
        <property type="interactions" value="217"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR68"/>
    <dbReference type="GeneID" id="18872001"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56131"/>
    <dbReference type="eggNOG" id="KOG1286">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_12_0_1"/>
    <dbReference type="InParanoid" id="G3AR68"/>
    <dbReference type="OMA" id="AITVGYW"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000017">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004762">
        <property type="entry name" value="Amino_acid_permease_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00913">
        <property type="entry name" value="2A0310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF1">
        <property type="entry name" value="GENERAL AMINO-ACID PERMEASE GAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="116"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="366"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="434"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="481"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="516"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="10">
        <location>
            <begin position="85"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW31243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="588" mass="63718" checksum="39A78EE5DF17980F" modified="2011-11-16" version="1">MAAEKDVHYITSREANSSDKGDALYVESYSQQEPATKLTGFAKFKDGFKRAKVEDLGIDENMTEAEKIGVLTANSPLSRSLKNRHLQMIAIGGSIGTGLFVGSGSNLSTGGPAGLLIAYCLIGTMIYCTVQSLGELAVTFPVSGAFVTYNSRFIDPSWGFAMAWNYAMQWLVVLPLELVAASLTIKYWDPNTNSAAYVTVFYVLIVAINFFGVRGYGEAEFIFSAIKVIAVIGFIILGIVLITGGGPQGGYLGGKYWHVDGAPFPNGAKGVVTVFVGAAFAFAGTELCGLAAAESANPRKSLPKACKQVFWRITLFYVICLTLIGMLVRYDNKRLLGASSVDATASPFVIAIENGGIKGLPSVMNVVIMVAVLSVGNSSVFGSSRTLAALAASNQAPKIFGYIDRQGRPLVGIIAQLVVGLLCYLAASPKEGEVFGWLLALSGLSSIFTWGSINLCLIRFRRALSVQGRDTSELTFTSQPGLIGAYWGMTLNIVVVCLQFWIAVWPLGGSPSAETFFNNFLTVPVVLIFYVGHKLYSRNWKLYIRAKDIDIDTGRREMDLDLVKQEVAEEKAYIASLPFYKRVYNFWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARE5</accession>
    <name>G3ARE5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Apurinic-apyrimidinic endonuclease 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_140201</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP endonuclease 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376530.1">
        <property type="nucleotide sequence ID" value="XM_007376468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARE5"/>
    <dbReference type="FunCoup" id="G3ARE5">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARE5"/>
    <dbReference type="GeneID" id="18870276"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140201"/>
    <dbReference type="eggNOG" id="KOG3997">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025885_1_0_1"/>
    <dbReference type="InParanoid" id="G3ARE5"/>
    <dbReference type="OMA" id="NPRGWAT"/>
    <dbReference type="OrthoDB" id="7663182at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017005">
        <property type="term" value="F:3'-tyrosyl-DNA phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00019">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.150:FF:000001">
        <property type="entry name" value="Probable endonuclease 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00152">
        <property type="entry name" value="Nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018246">
        <property type="entry name" value="AP_endonuc_F2_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00587">
        <property type="entry name" value="nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445:SF0">
        <property type="entry name" value="APURINIC-APYRIMIDINIC ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445">
        <property type="entry name" value="ENDONUCLEASE IV ENDODEOXYRIBONUCLEASE IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00729">
        <property type="entry name" value="AP_NUCLEASE_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00730">
        <property type="entry name" value="AP_NUCLEASE_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00731">
        <property type="entry name" value="AP_NUCLEASE_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51432">
        <property type="entry name" value="AP_NUCLEASE_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="9">
        <location>
            <begin position="11"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="337" mass="37571" checksum="C35E56642397FBA8" modified="2011-11-16" version="1">MSGGISKAVTNAMDIGANSFALFLKSPRKWVSPDIPDAEAEKFAELCKKYGYNPRTDILPHGSYFINLGNPDPEKEEKAYGSFLDDLKRCEKLNIGLYNFHPGSSLDSDHGEALERLAKNINKAIKETEFVKIVIENMAGHGNLIGSNLQDIRDVIDMVEDKSRVGVCLDTCHSFAAGYDISDEEKFNAFLKQFDDIIGAEYLSAIHLNDSKAPLGANRDLHQKLGYGFLGLEVFRVIANCDRLKTIPIILETPVGNDDSVYGEEIKLLEWLEGRSIDDGEFVAKTEELSKLGSKERDEHLKKFEAKTAKSEKKTGTKKRTVKVEITTMVSKKTKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARI4</accession>
    <name>G3ARI4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Plasma membrane ATPase</fullName>
            <ecNumber evidence="17">7.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">PMA1</name>
        <name evidence="20" type="ORF">SPAPADRAFT_56181</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>ATP + H2O + H(+)(in) = ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:20852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Cell membrane</location>
            <topology evidence="2 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.1" evidence="17"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376083.1">
        <property type="nucleotide sequence ID" value="XM_007376021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARI4"/>
    <dbReference type="FunCoup" id="G3ARI4">
        <property type="interactions" value="465"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARI4"/>
    <dbReference type="GeneID" id="18872012"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56181"/>
    <dbReference type="eggNOG" id="KOG0205">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002360_6_0_1"/>
    <dbReference type="InParanoid" id="G3ARI4"/>
    <dbReference type="OMA" id="VIEFHPF"/>
    <dbReference type="OrthoDB" id="116380at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02076">
        <property type="entry name" value="P-type_ATPase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000011">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000005">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000008">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 17" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="14 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 17" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="299"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="669"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="696"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="736"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="768"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="799"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="835"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="19">
        <location>
            <begin position="48"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EGW31305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="895" mass="97493" checksum="B7A45576C4A50F23" modified="2011-11-16" version="1">MSATEPTKEKVAKNLSDDEDEDIDQLVLDLQSNPAALDDDEEAQDEGTFKAVPEELLQTDPATGLSDDEVLKRRKRFGLNQMAEEQENLVLKFIMFFVGPIQFVMEAAAVLAAGLEDWVDFGVICALLMLNAFVGFIQEYQAGSIVDELKKTLANVALVVRNGQLIEIPANEVVPGDILQLEDGTVIPTDGRIVSEDCLLQVDQSAITGESLAVDKKHGDSCYSSSTVKTGEAFMVVTATGDSTFVGRAASLVNKASAGTGHFTEVLNGIGTTLLVFVIVTLLVVWVACFYRTVRIVPILRYTLAITIIGVPVGLPAVVTTTMAVGAAYLAKKQAIVQKLSAIESLAGVEILCSDKTGTLTKNKLSLHEPYTVEGVEADDLMLTACLAASRKKKGLDAIDKAFLKSLINYPRAKAALTKYKVLEFQPFDPVSKKVTAIVESPEGERIVCVKGAPLFVLKTVEDDHPIPEDVHENYQNTVAEFASRGFRSLGVARKRGEGHWEILGIMPCMDPPRDDTAATVNEARRLGLRVKMLTGDAVGIAKETCRQLGLGTNIYDADRLGLSGGGDMAGSEIADFVENADGFAEVFPQHKYNAVEILQSRGYLVAMTGDGVNDAPSLKKADTGIAVEGATDAARSAADIVFLAPGLSAIIDALKTSRQIFHRMYSYVVYRIALSLHLEIFLGLWIAILNRSLNIDLVVFIAIFADVATLAIAYDNAPYDPNPVKWNTPRLWGMSIILGIILAIGTWITLTTMLLPKGGIIQNFGGIDGILFLQISLTENWLIFITRAQGPFWSSIPSWQLSGAVLIVDIIATCFTLFGWWSQNWTDIVTVVRTWIWSFGVFCVMGGAYYLMSTSEGFDRVCNGKPRKQQTDRRSLEDFLVSMQRVSTQHEKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARQ1</accession>
    <name>G3ARQ1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SPX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_141183</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376582.1">
        <property type="nucleotide sequence ID" value="XM_007376520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARQ1"/>
    <dbReference type="FunCoup" id="G3ARQ1">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARQ1"/>
    <dbReference type="GeneID" id="18870352"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141183"/>
    <dbReference type="eggNOG" id="ENOG502SB0X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_333441_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARQ1"/>
    <dbReference type="OMA" id="IRYWNEF"/>
    <dbReference type="OrthoDB" id="5588846at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033254">
        <property type="term" value="C:vacuolar transporter chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006799">
        <property type="term" value="P:polyphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14474">
        <property type="entry name" value="SPX_YDR089W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF1">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE COMPLEX SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="762"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="798"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="838"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="8">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="590"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="607"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="862" mass="100733" checksum="3D12E4EEF43615A0" modified="2011-11-16" version="1">MKFGNNLQHLSIPEWKSYNIDYNDLKYQIREITQTKSTNLDSLHQSFVDNFDYINLFIQTKYGELIRKFDYYELCLQKIVKSCHQTDDDLRNCLVEVDEIFYQIIEISIVLKNLSKFVVIQKIAIKKIFKKLLKYYPVKNQSLKFILELKSYLVKNPNSLINFDLSPLTMKITNSINQIKNERKRLNSLVEGNSVGLARKTSMFSISSTLLSDTYYQNSIFSKSLPHQKPPQLQPHQHDNLTLESNFDLSVYLKKNFQSHALIPQDSNTMNDLILNFSIYLNLKNLSSTDLISYTYLMSEEEEDIFVEPSVIISSNSQENSLIIAHIGGLRKYSYCYLPNDIVQLFIHHLNDRENEEYKNRLIEYFQTHQVNHLTKKTIDHITSHNLVPKLKLFSKRSRYIINEDEEPGEENSEDGDDHSLASGYPDNHYLITLDSEISTTNNQKYVNDLTFPENHNDLDPFPHNHLAVYSNDIKLLNFEDSIETKINIKDQVIRNKYSSSCMRNLPKKIQSILTNNSLTLFKGLNFYQYQLSCYYNVIPDGKYVNNHYTNLLEINLLKNYENIESLNNQLSQENDLIKQKSRRIIEHKMSIKSLNTPTPSPDDNQKNSFGSSVHNSIFDEPGQANGEELSISPNELLRFNDETLLTGDEDDEDYGIYFEIPEPARDSSLNAFIDTLLNFKNRLFKKIEKKKKQIRFKQAENPFFYHNEEEQYLDPYTKLLACYHNSYHSPYDSINEEPPSYFHRNDFQIKYQLEYDQTLSYIYFSLNLIALFLSGIQLGIIYSIFDNSNQDHHFLIVNNIGLMIILILGIALSLIFSLISVNLMFYRYTPIPHKHQIISWSVFVIVALCCGWAGGLIIFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARW1</accession>
    <name>G3ARW1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_154438</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376156.1">
        <property type="nucleotide sequence ID" value="XM_007376094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARW1"/>
    <dbReference type="FunCoup" id="G3ARW1">
        <property type="interactions" value="316"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARW1"/>
    <dbReference type="GeneID" id="18870982"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_154438"/>
    <dbReference type="eggNOG" id="KOG4205">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_0_2_1"/>
    <dbReference type="InParanoid" id="G3ARW1"/>
    <dbReference type="OMA" id="RVCQNKF"/>
    <dbReference type="OrthoDB" id="1875751at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12577">
        <property type="entry name" value="RRM1_Hrp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000025">
        <property type="entry name" value="RNA-binding protein Musashi homolog 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034156">
        <property type="entry name" value="Hrp1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032:SF6">
        <property type="entry name" value="RNA-BINDING (RRM_RBD_RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032">
        <property type="entry name" value="RNA-BINDING PROTEIN MUSASHI HOMOLOG RBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="172"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="256"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="99"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="329"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="400"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="130"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="437"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW31378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="507" mass="55652" checksum="FE8603EE4D1D331D" modified="2011-11-16" version="1">MSNIDDDEALFEDLYGDDDETSTKPQVNDVPVATESAKADEKEASNTTTATTKTGDQQQASSAVAAAPAPAAPAAIPPQMDPAQYQQYLQYQQYYQQQQQQQQQGVSPIPGQSFPQAFPGMPPVPPPPVGASSGTPPAPGAGAAAGAPPPIPPPPAAPPVQHQPSNVGRDSGKMFVGGLNWDTTEEGLRDYFSKYGNVLDYTIMRDSATGRSRGFGFLTFEDPKSVDEVIKVDHILDGKLIDPKRAIAREEQDRVGKIFVGGIDPLVTEKEFYDFFAQYGSIIDAQLMVDKDTGRSRGFGFITYDSPDAVDRVTVNKYLSLKGRAMEVKRAEPRGQHQQNVMQQRQQQQQSSYYGAGAYGSPYGAGGAQMYQQQMNPAMNQEYMRYYQWFMYQQMAAGGAAAGTPDASADQQPAGQPLNPQQQANDTQPTEDQDPQEQNHDQMEENHQDYRGGYEDENPHRDQRRLNLPKGPKRAPPSGPGYNSRGRGGYHRRSRGYHPYSRGGGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS06</accession>
    <name>G3AS06_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_140188</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376633.1">
        <property type="nucleotide sequence ID" value="XM_007376571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS06"/>
    <dbReference type="FunCoup" id="G3AS06">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AS06"/>
    <dbReference type="GeneID" id="18870272"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140188"/>
    <dbReference type="eggNOG" id="ENOG502S7B4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056574_2_0_1"/>
    <dbReference type="InParanoid" id="G3AS06"/>
    <dbReference type="OMA" id="STTDMEC"/>
    <dbReference type="OrthoDB" id="10255128at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050849">
        <property type="entry name" value="HAD-like_hydrolase_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28181:SF1">
        <property type="entry name" value="COLD TOLERANCE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28181">
        <property type="entry name" value="UPF0655 PROTEIN YCR015C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12710">
        <property type="entry name" value="HAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="256" mass="29430" checksum="9E078DADB045A283" modified="2011-11-16" version="1">MPRMKPRIVISDWDETITAKDTIKYVAETAYINKPNCTPPFSYFTNVYLDAYNKYSNSFGPRSNLDQEIKFQSGMAVVENTSIQALADHRIFSGLTKDQFRLQANKIELRPGFVEFLTKCQTLDIPFVILSVNWTRIPIIECLKLHGFSVDDDKIKVIANEFVFEKVEDIEITTGDWDNSINVRISSDKLNIVERLRRDKQLVYIGDSSNDLLPLLEADIACAIQGTKIVDILDKYSLNQPNYHIGDWHNFINLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS64</accession>
    <name>G3AS64_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_142720</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377056.1">
        <property type="nucleotide sequence ID" value="XM_007376994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS64"/>
    <dbReference type="STRING" id="619300.G3AS64"/>
    <dbReference type="GeneID" id="18870477"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142720"/>
    <dbReference type="eggNOG" id="ENOG502S3G3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_960004_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS64"/>
    <dbReference type="OMA" id="QIDEIRY"/>
    <dbReference type="OrthoDB" id="2430210at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="252"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="264"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="287" mass="32180" checksum="73303269AA62D2AB" modified="2011-11-16" version="1">MSNPPPTQPFASLALSQEQFAALLSRDRRSDTRHPALLKNPRDPELIYQFINEVELFAPLHHVGEGEVVEYALKSFGADLLRWYAERMSTIASWIDLKTQLVQCFVPPRFEDNMAEKLYSLQQTSTVEAYTSEFRRIKGYLTHESATDRQVKNWYVKGLSPALFPYLIEDVYNPQVSFDSLAYKAQIRADLTGPVPVVGFYPRYDVPGHIPPAPAPSTTAVDPDAMDIDALAVRRKKLSPQERAHLMATGGCFACRRPGHHASQCAWSSKGRSAGNRMSPVNTSPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASB8</accession>
    <name>G3ASB8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Histidine biosynthesis trifunctional protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="19">Phosphoribosyl-AMP cyclohydrolase</fullName>
                <ecNumber evidence="19">3.5.4.19</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="19">Phosphoribosyl-ATP pyrophosphohydrolase</fullName>
                <ecNumber evidence="19">3.6.1.31</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="19">Histidinol dehydrogenase</fullName>
                <shortName evidence="19">HDH</shortName>
                <ecNumber evidence="19">1.1.1.23</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="22" type="primary">HIS2</name>
        <name evidence="22" type="ORF">SPAPADRAFT_141809</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:20049"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="EC" id="3.5.4.19"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 19">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-5'-AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22828"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="3.6.1.31"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 19">
            <text>L-histidinol + 2 NAD(+) + H2O = L-histidine + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:20641"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 19">In the C-terminal section; belongs to the histidinol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.19" evidence="19"/>
    <dbReference type="EC" id="3.6.1.31" evidence="19"/>
    <dbReference type="EC" id="1.1.1.23" evidence="19"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376691.1">
        <property type="nucleotide sequence ID" value="XM_007376629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASB8"/>
    <dbReference type="FunCoup" id="G3ASB8">
        <property type="interactions" value="372"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASB8"/>
    <dbReference type="GeneID" id="18870399"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141809"/>
    <dbReference type="eggNOG" id="KOG2697">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4311">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006732_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASB8"/>
    <dbReference type="OMA" id="SVFIGAW"/>
    <dbReference type="OrthoDB" id="1703565at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004399">
        <property type="term" value="F:histidinol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004635">
        <property type="term" value="F:phosphoribosyl-AMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004636">
        <property type="term" value="F:phosphoribosyl-ATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06572">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11546">
        <property type="entry name" value="NTP-PPase_His4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1080:FF:000002">
        <property type="entry name" value="Histidine biosynthesis bifunctional protein HisIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.810:FF:000002">
        <property type="entry name" value="Histidine biosynthesis trifunctional protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1980:FF:000001">
        <property type="entry name" value="Histidinol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1300:FF:000002">
        <property type="entry name" value="Histidinol dehydrogenase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1980:FF:000002">
        <property type="entry name" value="Histidinol dehydrogenase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1080">
        <property type="entry name" value="MazG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.810">
        <property type="entry name" value="Phosphoribosyl-AMP cyclohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01024">
        <property type="entry name" value="HisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008179">
        <property type="entry name" value="HisE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016298">
        <property type="entry name" value="Histidine_synth_trifunct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001692">
        <property type="entry name" value="Histidinol_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012131">
        <property type="entry name" value="Hstdl_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021130">
        <property type="entry name" value="PRib-ATP_PPHydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002496">
        <property type="entry name" value="PRib_AMP_CycHydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038019">
        <property type="entry name" value="PRib_AMP_CycHydrolase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00069">
        <property type="entry name" value="hisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03188">
        <property type="entry name" value="histidine_hisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256:SF2">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS TRIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256">
        <property type="entry name" value="HISTIDINOL DEHYDROGENASE HDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00815">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01502">
        <property type="entry name" value="PRA-CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01503">
        <property type="entry name" value="PRA-PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001257">
        <property type="entry name" value="His_trifunctional"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00083">
        <property type="entry name" value="HOLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101386">
        <property type="entry name" value="all-alpha NTP pyrophosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141734">
        <property type="entry name" value="HisI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00611">
        <property type="entry name" value="HISOL_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 19" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="13 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="16 19" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="11 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="15 19" id="KW-0520">NAD</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phosphoribosyl-AMP cyclohydrolase" evidence="21">
        <location>
            <begin position="212"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="326"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00008260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR001257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF01502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="EGW30658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="856" mass="93512" checksum="D4AFEAB36D86AE9E" modified="2011-11-16" version="1">MSFPVLPLISSPTEDATIAEFSLVGQVLFPWTSVTVSKHWLQQFPPDLEVYVDATTDSEITVDQVVALLNTGVKQVFIPAARYHEIVAESSLPTERFAVQFDKDDVSQDLLKSKSSFIFKSKVANFKSYNDDENRQIYIRDDSLSQESANELAQSGYTPVVSATKLTTKKQEAGRISVSSIFISTLTTDRPDGLYTTLITTPAPSYTALGIVYSSKESIAAAIEEKVGVYQSRKRTDELWYKGKTSGATQSLVSISKDCDSDVVKFIVVPKEGYGFCHRQDNFTCFGDGQLAGTETSFGKGLAKLDTTLAARLQNAPEGSYTQRLFKDEKLLIAKLKEELDELIEAKGKEEVAWECADLLYFAMVWCIKNGVRLSDVERNLDIKSLKVTRRKGDAKPAYVNNEQKQAQPEALDFSLETIKVDDINADSEDLNRAMTRPVQKTADIMKLVLPIIENVKKNGDKALIELTAKFDGVELSNPVLNAPFPSELMQISQEMKDAIDLSISNIEKFHAAQLPKEKVMTVETAPGVFCSRFAKPIENVGLYVPGGTAVLPSTAMMLGVPAKVAGCKNIIIASPPARATGKLTPEVVYVAHKLGAKCIVMAGGAQAVTAMAYGTESVLKCDKILGPGNQFVTAAKMYVQNDTQALCSIDMPAGPSEVLVIADEYADADFVASDLLSQAEHGIDSQVILIGIDLSDSKLKEYQDAVARQASVLPRKEIVAKCLSHSYILLVKTYEEAFELSNKYAPEHLILQIENAKSYVPEYVDNAGSVFIGALSPESCGDYSSGTNHTLPTYGYARQYSGVNTATYQKFITAQDVTKEGLESIGKAVMTLAAVEGLEAHRRAVEVRMEKLGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASB9</accession>
    <name>G3ASB9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peroxisomal targeting signal receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Peroxin-5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">SPAPADRAFT_62526</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Receptor that mediates peroxisomal import of proteins containing a C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type). Binds to cargo proteins containing a PTS1 peroxisomal targeting signal in the cytosol, and translocates them into the peroxisome matrix by passing through the PEX13-PEX14 docking complex along with cargo proteins. PEX5 receptor is then retrotranslocated into the cytosol, leading to release of bound cargo in the peroxisome matrix, and reset for a subsequent peroxisome import cycle.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts (via WxxxF/Y and LVxEF motifs) with PEX14; promoting translocation through the PEX13-PEX14 docking complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxisomal targeting signal receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376692.1">
        <property type="nucleotide sequence ID" value="XM_007376630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASB9"/>
    <dbReference type="FunCoup" id="G3ASB9">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASB9"/>
    <dbReference type="GeneID" id="18874360"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62526"/>
    <dbReference type="eggNOG" id="KOG1125">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013516_3_0_1"/>
    <dbReference type="InParanoid" id="G3ASB9"/>
    <dbReference type="OMA" id="WEEQFKQ"/>
    <dbReference type="OrthoDB" id="10006023at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005052">
        <property type="term" value="F:peroxisome matrix targeting signal-1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140597">
        <property type="term" value="F:protein carrier chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000218">
        <property type="entry name" value="Peroxisomal targeting signal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024111">
        <property type="entry name" value="PEX5/PEX5L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130:SF0">
        <property type="entry name" value="GH08708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR PEX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="14" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0882">Thioester bond</keyword>
    <keyword evidence="9 18" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="13" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="repeat" description="TPR" evidence="18">
        <location>
            <begin position="342"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="18">
        <location>
            <begin position="452"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="18">
        <location>
            <begin position="486"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="22"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="86"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00046106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EGW30659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="605" mass="68921" checksum="DD04A57B9EF758DE" modified="2011-11-16" version="1">MSFVGGGSECSVNGNAVAQFNKHTQQDRSLQQQHSQGIPIQQQPGFKHDNVMNLRDRQNLEQFMNNNNSQANAFQFQSMRHELNTMQHHPSPQTSNWSNEFKLSPSPVTQNAKIQTMGKTSSLNGQWAGEFQQHQPPPQQQQQQQPQQYNTGMMMGASRPMMMGMGPMMTGPMHQQNQHQHQHQHQQPVAAQEQNVDWDNQFKEIEELTQQAKEAEQVKEESPDIIIDDKYQATFQEVWDGLNDTSLEEDFINQQYEDFKHTQMDTLPADLTQWERDFARYVSTRAHFGDYQFEDTKNNQFMDLPKEADPYEIGLQLMENGAKLSEAALAFEAAIQRNENHVDAWLKLGEVQTQNEKEIAGISALEKCLELNPENLDALMTLAISYINEGYDNAAFATLERWISTKYPQISARAREENPKISDEDRFSLNKRVTELFLQAAQLSPNSANMDADVQMGLGVLFYANEDFDKTIDCFKAALSIRPDDAVLWNRLGASLANSNRSEEAVDAYFKALQLKPTFVRARYNLGVSCINIGCYKEAAEHLLSGLSMHQVEGVDANVSTLNHNQSTSLTETLKRAFIAMERRDLLELVKPGMDLEQFRGEFTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASF4</accession>
    <name>G3ASF4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pyrimidine 5-nucleotidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_142381</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377105.1">
        <property type="nucleotide sequence ID" value="XM_007377043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASF4"/>
    <dbReference type="FunCoup" id="G3ASF4">
        <property type="interactions" value="566"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASF4"/>
    <dbReference type="GeneID" id="18870444"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142381"/>
    <dbReference type="eggNOG" id="KOG3109">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059493_0_1_1"/>
    <dbReference type="InParanoid" id="G3ASF4"/>
    <dbReference type="OMA" id="WEANEKS"/>
    <dbReference type="OrthoDB" id="1065058at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008252">
        <property type="term" value="F:nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009166">
        <property type="term" value="P:nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006206">
        <property type="term" value="P:pyrimidine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010237">
        <property type="entry name" value="Pyr-5-nucltdase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052791">
        <property type="entry name" value="SSM1_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01993">
        <property type="entry name" value="Pyr-5-nucltdase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47438">
        <property type="entry name" value="PHOSPHATE METABOLISM PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47438:SF1">
        <property type="entry name" value="PHOSPHATE METABOLISM PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01132">
        <property type="entry name" value="C1.5.3:_5'-Nucleotidase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="283" mass="33246" checksum="A95C0BFE3D66C773" modified="2011-11-16" version="1">MTTSKNQAITYKNPEVPDDQRPGRSVHLPFGFGPIPKDLANKKIFYFDIDNCLYRRSTQIFEMMQVKIHEYFKHNLQLNDEDAHNLHMNYYKMYGLALEGLVRNHEIDALDYNSKVDDALDLHAVLHYDQDLRDCLIKLKETHKFDFFWLVTNAYKNHALRVVSFLGVGDLFDGLTYCHYSKFPIVCKPMKEYYYNCFELTQIDYKDPTVMAKQYFVDDSEINVKAAYDLGMGHVFHYVELDSDLVKMKNKPDFAKYYGTGDNSDKSKIKIIRHIIDLPDVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASF7</accession>
    <name>G3ASF7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Translation initiation factor eIF4e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_52253</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377108.1">
        <property type="nucleotide sequence ID" value="XM_007377046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASF7"/>
    <dbReference type="STRING" id="619300.G3ASF7"/>
    <dbReference type="GeneID" id="18871651"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52253"/>
    <dbReference type="eggNOG" id="KOG1669">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_574897_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASF7"/>
    <dbReference type="OMA" id="SNERVFG"/>
    <dbReference type="OrthoDB" id="590761at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019770">
        <property type="entry name" value="TIF_eIF_4E_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF18">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E HOMOLOGOUS PROTEIN, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00813">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="343"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="380"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="43772" checksum="F9760B5B78D9512C" modified="2011-11-16" version="1">MSDNLRRAESLFNRIMNQTQESTPSTQTRSPSGSGPSYAPTTKPNGSTVNGTSHEESSSVVLPKQDPVKLSHETLALIPESHHILPYCWTIWHHSRSRKPKEAEEGASVASVPADANIIANVDTYLQTTNELEFVDLLGNKIKHIGSLEQLWVALSSIKKTYELTTGTEFLVFKAGINPVWEDPINSKGGRWVFRFSRKIFGDDKESIVKIRKRTSLIWERLMLKTMTGSLIPESNSEEFQSLLLNDICGIVLSVRKDEDIISIWNSNLNFHKKSANSTDDSKKKLTSFQARRIICDSILRVIRECDLISQGSDCVGTLDSGSNERVFGVSFEYRLHADNENVPSGNAKYNGHHHHSHHGYNAHHQRRYNRGGSNNSNYKNEQQPGEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASG5</accession>
    <name>G3ASG5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63010</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377116.1">
        <property type="nucleotide sequence ID" value="XM_007377054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASG5"/>
    <dbReference type="GeneID" id="18874552"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63010"/>
    <dbReference type="HOGENOM" id="CLU_1476015_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASG5"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442337" evidence="1">
        <location>
            <begin position="23"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="183" mass="21014" checksum="7BC8C83A80D6BA0C" modified="2011-11-16" version="1">MLLWANTSALCILFCTLQATLSAAMLSQNTNTGATRNQLVKRDTSSKTEYRIISVKIDQPGRCKEGAYKDVNWEDTGFSTVVENDTELAKYRYDNKDDNKYCLYWYILLNTGDANEARKQANEYYEKTKSTISRLKTEFNYKLGEDTSGDYKTPYPVNKNKLNEPYVKITETANDWSIAEQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASL3</accession>
    <name>G3ASL3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_51911</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376732.1">
        <property type="nucleotide sequence ID" value="XM_007376670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASL3"/>
    <dbReference type="GeneID" id="18871609"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51911"/>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029128_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASL3"/>
    <dbReference type="OrthoDB" id="4073735at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF22">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN COMPLEX ACID LABILE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="413" mass="48203" checksum="1C20422C7CF64C4E" modified="2011-11-16" version="1">MVTDQGDWISTFLTTYFVCLPDEIVIEILNNVTFDHLVNFFMHFPDYNIQQLTRENYSNEVHFVNRPTVTKSYGRSGYKHRFLEFHGKEAIFRFMDEFPDLVPKRIILFNEFGNFEPIRDIMETYPDRIGQVKEMKISLEKCEITIADFQCLTSFPNLTKLNFLRMDLNLIRSVLVNNSEFAQHPKLDEITFWQHGIYDWSNVWFPRTLKSLDLSWNNMIDISTLSIPGTVRRVCLNYSNLDSFKVLKTKIPDTLTELGLVGNQFETLALNQLPTELRFLDLSSNKISSVVGSNWPKELTELRISGNKLDNEALEYINDIIGWPQSITNLDISLNQFTLLENLSKLPKSLQVLDISKNSLIWNDPQLINFHFPDHLTKLNLSDCEIDSVRYFRFPSSLEKLILKSNEEDIGSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT93</accession>
    <name>G3AT93_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_68090</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376889.1">
        <property type="nucleotide sequence ID" value="XM_007376827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT93"/>
    <dbReference type="GeneID" id="18875195"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68090"/>
    <dbReference type="HOGENOM" id="CLU_1349642_0_0_1"/>
    <dbReference type="InParanoid" id="G3AT93"/>
    <dbReference type="OrthoDB" id="4025857at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="203" mass="23669" checksum="C9E9452C9E7F6E06" modified="2011-11-16" version="1">MDCLTHNNMIAENRQYSFDSQRVSSPEIDSSSCSLHRSVDSSMFTSHESFSQQQFHGMESWNYENSNLVGSNSNNNLCDQLQIETCDESTLDSEPTPTWDKHTTTYRTQDNTLKLQIWIDDNKLSFKLRKDRLESTTELVNVILFKLKVKLNMYPDRLQITFKDQSLNPISLFEHGETQTPELLLEYIQLKKTIYIRAIKQEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT97</accession>
    <name>G3AT97_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CSC1/OSCA1-like 7TM region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_62757</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376893.1">
        <property type="nucleotide sequence ID" value="XM_007376831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT97"/>
    <dbReference type="GeneID" id="18874453"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62757"/>
    <dbReference type="eggNOG" id="KOG1134">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002458_0_2_1"/>
    <dbReference type="InParanoid" id="G3AT97"/>
    <dbReference type="OMA" id="HENWRAT"/>
    <dbReference type="OrthoDB" id="1689567at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF5">
        <property type="entry name" value="RE44586P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="2">
        <location>
            <begin position="40"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="484" mass="55872" checksum="9CEBA4433AA52277" modified="2011-11-16" version="1">MAAQTVLDPRVYKLIVNLAPAPKDIIWENLKLTYNQKLFKSYLITFLIVLSYGFIIFLVVPLTSLLDLKTISKFFPSLGKFIGQSEWLTTFVTGILPPLLFTLLNVSFPYFYQWLSQYQGHSSNSDVELSTLSKNFFFIFFNLFLIYVAAGTFWDYISYISDTTKIAVQLATSLKRMALFYVDLILLQGLTMFPVKLLQVSDFLVLNILGKCLPRMFLRTPRNYRTYYYTPQIFDFGIHLPQHILIFMIILIYSVVSTKIVTCGLVYFIMGLLVYKYQLVYNFVHPPHSTGKVWPMIFRRVMLGLIIFQLFMCGTLALESAILLSIMCTPLILVTLFICWNFETYYVPLNTFIALRAILNPYDYDKVFDDDQSFNSEEEQSVSLGPSSSDVGPIDESSCLLEEHARHKRRKSTIDEEREEDTNYTYPYLIDPLDGPWVGFTGDSVTMVEYRVDGSVLESGYRTGPLEGEFETIVNKRLVVSEWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATE3</accession>
    <name>G3ATE3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Kynureninase</fullName>
            <ecNumber evidence="4 5">3.7.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Biosynthesis of nicotinic acid protein 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">L-kynurenine hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">BNA5</name>
        <name evidence="6" type="ORF">SPAPADRAFT_56854</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>3-hydroxy-L-kynurenine + H2O = 3-hydroxyanthranilate + L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25143"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36559"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:58125"/>
            <dbReference type="EC" id="3.7.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>L-kynurenine + H2O = anthranilate + L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16813"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="3.7.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Amino-acid degradation; L-kynurenine degradation; L-alanine and anthranilate from L-kynurenine: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the kynureninase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.7.1.3" evidence="4 5"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376939.1">
        <property type="nucleotide sequence ID" value="XM_007376877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATE3"/>
    <dbReference type="FunCoup" id="G3ATE3">
        <property type="interactions" value="209"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATE3"/>
    <dbReference type="GeneID" id="18872072"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56854"/>
    <dbReference type="eggNOG" id="KOG3846">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_4_0_1"/>
    <dbReference type="InParanoid" id="G3ATE3"/>
    <dbReference type="OMA" id="LPGWNSH"/>
    <dbReference type="OrthoDB" id="5978656at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00329"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00334">
        <property type="reaction ID" value="UER00455"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097268">
        <property type="term" value="C:cytoophidium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030429">
        <property type="term" value="F:kynureninase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097053">
        <property type="term" value="P:L-kynurenine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000031">
        <property type="entry name" value="Kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01970">
        <property type="entry name" value="Kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010111">
        <property type="entry name" value="Kynureninase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01814">
        <property type="entry name" value="kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14084">
        <property type="entry name" value="KYNURENINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14084:SF0">
        <property type="entry name" value="KYNURENINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22580">
        <property type="entry name" value="KYNU_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038800">
        <property type="entry name" value="KYNU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4 5" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="3 4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="146"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR038800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="468" mass="53004" checksum="D49031381265B642" modified="2011-11-16" version="1">MSLQQANQLDEEFPTYKSEFAIPTFETLGISPPSVTNGPTLTPSTESIYLCGNSLGLMPKQTKTAILAELTAWEHRAVESHFNHPSSTPWVDIDLPLLPLVAPIVGAKENEVAVMGSLTANLNALLIHFYKPKGKRTKILFEKQAFPSDFYAFLNLVKLFGYDESHLIQVGVEKGKTHLTTEQIKRFVDENIDELALVCFPGIQYYTGQLFDIAEITRYIKEKSSDIKVGWDLAHAVGNVPLHLHDWNVDFAAWCSYKYLNSGPGGIAGIYVHEHYTRDNTTGHFQPRLAGWWGNNSDERFKMLEKFDPINSALSYRMSNPSVIDTVAVRSSLELFAKVGGVETLRKKSIKLTQFLQDLLTSSKYYIKQDDTDGSKLGFKILTPLNPSERGCQLSILFQPHFDDKEKNVMERVNKYLHDRGIICDERRPDVIRLAPLPLYNTFTETYHASQRLFEALDAIEQNHTLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATH5</accession>
    <name>G3ATH5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Endonuclease III homolog</fullName>
            <ecNumber evidence="8">3.2.2.-</ecNumber>
            <ecNumber evidence="8">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <shortName evidence="8">DNA glycosylase/AP lyase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NTG1</name>
        <name evidence="11" type="ORF">SPAPADRAFT_156404</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the Nth/MutY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="8"/>
    <dbReference type="EC" id="4.2.99.18" evidence="8"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376971.1">
        <property type="nucleotide sequence ID" value="XM_007376909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATH5"/>
    <dbReference type="FunCoup" id="G3ATH5">
        <property type="interactions" value="499"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATH5"/>
    <dbReference type="GeneID" id="18871049"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_156404"/>
    <dbReference type="eggNOG" id="KOG1921">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_4_3_1"/>
    <dbReference type="InParanoid" id="G3ATH5"/>
    <dbReference type="OMA" id="RGKRCDL"/>
    <dbReference type="OrthoDB" id="2099276at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000703">
        <property type="term" value="F:oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000001">
        <property type="entry name" value="Endonuclease III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03183">
        <property type="entry name" value="Endonuclease_III_Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004036">
        <property type="entry name" value="Endonuclease-III-like_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030841">
        <property type="entry name" value="NTH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286:SF1">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
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    </dbReference>
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        <property type="entry name" value="ENDONUCLEASE_III_2"/>
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    </dbReference>
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    <keyword evidence="2 8" id="KW-0227">DNA damage</keyword>
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    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="10">
        <location>
            <begin position="106"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="292"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00044632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="329" mass="36686" checksum="9DD2FD9322AA03CA" modified="2011-11-16" version="1">MAKKRVVETVAAVKRIKTEPVDTKPRYVKYLDIEHPNVFAKADPADASVGPPNWVDLYNRVVAMRAKFMAPVDTQGCERMPETISPGVSQSNSKVYRFQLLISLMLSSQTKDEVNFDAMTKLHKAMLEKGYKDGLCIEAVLATSESDIDKLIAKVGFHNRKAGYIKRSCQMLIDSHNGDIPTTIEEITALPGVGPKMGYLLLQAGWGINSGIGVDVHLHRLAQMWGWVSKKATTPEKARLELEDWLPRQYWKDVNPLMVGFGQVVCGSRAKNCDVCSLNTMCAGKDRKLAGKEVSKERLEKLKAQRADLSVLVEEVNVKQEIKQESLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATT5</accession>
    <name>G3ATT5_SPAPN</name>
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        </recommendedName>
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        <name evidence="7" type="ORF">SPAPADRAFT_143440</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996505">
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATT5"/>
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    </dbReference>
    <dbReference type="STRING" id="619300.G3ATT5"/>
    <dbReference type="GeneID" id="18870548"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143440"/>
    <dbReference type="eggNOG" id="ENOG502S1J0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_828994_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATT5"/>
    <dbReference type="OMA" id="DWPGWLM"/>
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    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035838">
        <property type="term" value="C:growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051380">
        <property type="entry name" value="pH-response_reg_palI/RIM9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28013">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
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        </location>
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    </feature>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="30569" checksum="1B0621B00400A2C6" modified="2011-11-16" version="1">MLKPLLILLCLSLICWIIQLLPVISVPITPTTTNIYLSKYQNFTYGVFGICQNNICSNPRIGYPSVNSTFYSLTGDVGESTSAGVVLPSSVTYTISKLLVVHVMAFSFTSMLIIIISGLIMLQVYRKRKNQARRSNAGINLMLISTLFSFLTNLLGFLVDLLLFTPNLSYLGWLQLIPIVSMVLITTMLCFIKRTIVSRRFFENDEQTYHANDDMRMMRKNVIDRFYNDNASDDGFYVVTDGFYTRNNHDIRQSHTSEEYSLDSLHTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATU8</accession>
    <name>G3ATU8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CHCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63182</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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        <lineage>
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            <taxon>Dikarya</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="619300.G3ATU8"/>
    <dbReference type="GeneID" id="18874630"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63182"/>
    <dbReference type="eggNOG" id="KOG4090">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093520_0_1_1"/>
    <dbReference type="InParanoid" id="G3ATU8"/>
    <dbReference type="OMA" id="CDADARN"/>
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    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055304">
        <property type="entry name" value="CHCHD2/10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523:SF2">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2/NUR77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="15571" checksum="F4B29B75BE22697E" modified="2011-11-16" version="1">MARQRRSAPAPRQQTRSAHTAAAPAYHAPPPPAAAAAHPAQAAQPRQPGMFAQMASTAAGVAVGSAVGHTIGAGITGMFGGSSAAPAEAPQQQVAPIQQQQQYQEQAKHCDADARNFTRCLEDNSGNMQICDYYLQQLKACQEASRQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUI1</accession>
    <name>G3AUI1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63347</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377508.1">
        <property type="nucleotide sequence ID" value="XM_007377446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUI1"/>
    <dbReference type="GeneID" id="18874713"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63347"/>
    <dbReference type="HOGENOM" id="CLU_1478529_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUI1"/>
    <dbReference type="OrthoDB" id="10636010at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="183" mass="20301" checksum="BECA5E82F6384D13" modified="2011-11-16" version="1" fragment="single">MDFEFPTTETTSTASTISSTTSTTTKFKSKLRLLKHELKTTSFHSNNNFIGISQSQPTTPTSNHTTRFRTSSASLSTTLPLYDAYEYDQIDPLDQIGSPSTPMNHHQPEEEMMIPPLPPTDFSFHSHQSSVFSIYPGFSDDENDGEGGDEESLQITQRVRSSYASSTSSISTSGDKRQSIKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUR0</accession>
    <name>G3AUR0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_63445</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377587.1">
        <property type="nucleotide sequence ID" value="XM_007377525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUR0"/>
    <dbReference type="GeneID" id="18874755"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63445"/>
    <dbReference type="HOGENOM" id="CLU_1705352_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUR0"/>
    <dbReference type="OrthoDB" id="4014264at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="154" mass="17670" checksum="4EC2C9619C9E5203" modified="2011-11-16" version="1">MRQDETSSVHRSSRIILEAMVDASFGVEFGFGSILDDNIVEYTLRYRNVFKVFKSRYVESSMGCSTNKKGHAARVFGFIPIIHNKMRVRKIEYDAEEGTISKDDEFKSLPPVRKLSNGVLKTVCKVGTRDQLRCHENSGKFIDEYKNQFSFNVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUU9</accession>
    <name>G3AUU9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_158397</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377805.1">
        <property type="nucleotide sequence ID" value="XM_007377743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUU9"/>
    <dbReference type="GeneID" id="18871125"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_158397"/>
    <dbReference type="eggNOG" id="ENOG502RQHT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090369_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUU9"/>
    <dbReference type="OrthoDB" id="3365224at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="160"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="174" mass="19496" checksum="F7DE9018C260DBF0" modified="2011-11-16" version="1">MSQRVPNYYYPSTKCKYLPTRAISRGQFRGFNDLRRFQCFNSGCNSSTSEFALCEYCRHVPYVSSPLRYSCGVQNITPTASSNRGSFIPRRSKSSSLSSSSSSSASSSRVDTPDENDKIVSSSGNEISNKITEFFDHLEVSKCQLKQRIMKGKLLKRRSSSSSSTNENLTWNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUW2</accession>
    <name>G3AUW2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_143909</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377819.1">
        <property type="nucleotide sequence ID" value="XM_007377757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUW2"/>
    <dbReference type="GeneID" id="18870583"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143909"/>
    <dbReference type="eggNOG" id="ENOG502REGU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_937226_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUW2"/>
    <dbReference type="OMA" id="ICILRFQ"/>
    <dbReference type="OrthoDB" id="4015819at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="282" mass="33480" checksum="7E241810DCCF655D" modified="2011-11-16" version="1">MEKPISDLPPTKNRYIQVLDLYLSNIIHNPIFTSVAVLISFCYLQCKQWDWLMISSHSFEFIFVEDYEFDILSNIINLIFIFRFVYYIKLNLIGEIALFLVQNFACYKYVLMKDGISFFKDTDMSMFLKSCIALQVIFPWLILLSCIVQRMPWNKTLVDCNTNYLPLFMSQSFMFVNTTFPLLDNSFKNYELLYKLACFSLKFYLVFQFVLYEFVLINYEHEDVVDYSVYYREVSCFNEKGRQGKSNFNLVKKYAMTVVCLFIIVIDLGFIGAVHKNVYNML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV03</accession>
    <name>G3AV03_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Rhomboid-type serine protease 2</fullName>
            <ecNumber evidence="4">3.4.21.105</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Rhomboid protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_63697</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Probable rhomboid-type serine protease that catalyzes intramembrane proteolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">cis-Golgi network membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="4"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377843.1">
        <property type="nucleotide sequence ID" value="XM_007377781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV03"/>
    <dbReference type="FunCoup" id="G3AV03">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV03"/>
    <dbReference type="GeneID" id="18874873"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63697"/>
    <dbReference type="eggNOG" id="KOG2632">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071084_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV03"/>
    <dbReference type="OMA" id="NTYPIVH"/>
    <dbReference type="OrthoDB" id="10257275at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066:SF1">
        <property type="entry name" value="RHOMBOID PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="14">
        <location>
            <begin position="59"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW30077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="273" mass="30636" checksum="35AC28CF17B414EA" modified="2011-11-16" version="1">MFSLDYKTHWSKFNQTPALTAGLILFTCILFLLNVYDVMDKSPFILYPNSPLELNLNAISLYLLFHTNFGHWFINVLGIATPLAMYEATHGTIYTGITLNLLAVITALQYCLVGQILYPHTGVIGLSGIIFSLLSYTSYKEHQNRPVIHTYRVSSYEIKIYTLAVPFIFLVFMTILMPGSSFFGHLAGVFAGFLLGMGYLKKLFPPSTAIIWIENKVEPVIKLINKLVTFVKEEDAINKRSVTYVSMFSSDLETGSVNTRPPTYSAETRVLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV25</accession>
    <name>G3AV25_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription regulator Rua1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_68925</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377864.1">
        <property type="nucleotide sequence ID" value="XM_007377802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV25"/>
    <dbReference type="GeneID" id="18875247"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68925"/>
    <dbReference type="eggNOG" id="ENOG502SZ9T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034746_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV25"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028012">
        <property type="entry name" value="Rua1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14616">
        <property type="entry name" value="Rua1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator Rua1 C-terminal" evidence="2">
        <location>
            <begin position="369"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="530" mass="60648" checksum="8B9AAD2EEA3441D7" modified="2011-11-16" version="1">MTSTISNETMEFSFSDDLITNTNSTTNTNSASANYHREDFSFDYFDQNPVEPKKTLEDYHHIDPEQPSNISKEEFSSLDDEFLAMFQDPIAHSETVDNTICPSTNMNWYMELALNNSQTENTSTLIPHKEEPHNNVQEEENMMNLFEATCKELDEWKSSINLEEFLPELVSSTKHKEVYSKSQSPLQLITPTSINTSALNTGDPWTGLNFDLNPEPATTQHCFPPVPPESSYSSTIEPQHFQHPVITLPERKSIPPTEHKSISSPVPKPITASTKKRKLDSRTTKLDFSHIDVPSIADLSYSAKLANHSPNVPTPAQCRILNEKLIQPYSCSETCHIQRTLYLREDLEVLLKPEFRGEFELSLNQNFKKTSYEAQYILTKFDRYGKPDNTTRAGLCPYCEKVQFFGLKNSSYGNHLAYKHGILTNGASVPDPKYYGKYKFKKGEYEEPEKKKRRTNAHLLEREGVLCTSCWQILEVNCTSRSSVLGHYLRHYRDSHVGNKKETSTTTTSAFSNKYDPNVVQFTSKWKTKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV28</accession>
    <name>G3AV28_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_52933</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377868.1">
        <property type="nucleotide sequence ID" value="XM_007377806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV28"/>
    <dbReference type="FunCoup" id="G3AV28">
        <property type="interactions" value="158"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV28"/>
    <dbReference type="GeneID" id="18871737"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52933"/>
    <dbReference type="eggNOG" id="ENOG502S0PT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030290_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV28"/>
    <dbReference type="OMA" id="HANNHHF"/>
    <dbReference type="OrthoDB" id="4091299at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13270">
        <property type="entry name" value="PROTEIN C20ORF116-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="357"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="437"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="601"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="621" mass="68646" checksum="9106EE99A65805EC" modified="2011-11-16" version="1">MSSTTDSNPLLVDPSIVSISDQPNSTSVSTSTSTTQLHTSSIHNLEPGNALSKLLDSAMAHAAKESTPDIVTSDAPAVSASSSGSSDKVSSESAIFYSTIPEKKQGMEGVYTVDYLLSLKNSPDIPDFKDKLPEKSFWRSNKPRESYNQASATTGRHHNKKNSYNRRSHQNETWERGKSDNLGFARSNELDSMSMEKISQLLGESNDDLGEPEWEDTKKNGNDDSLGLMGMGQTVEDFERWKSKMKLEQRRRNGEIIDESLEAENTVHAAAAHAGNEVDNFFSFVKTKENDDESHPEEASHTPEHDNHEESKNKSSRFLSFFPQPASEKQQPQQAPAGPPPGMSKFFRGPPGPQPQQSPQQQQPQQQQVPSQQQQQQQPQQQQPQQQQQQNQPQHQQPPQQRPQQQHPQAFPFPPSMPPNTNDNFFMSLLNKKEPGQETPEQKQQQPQPSPQQQQQLKQQPSPQQGQPGQQQIPPWMRQLAYGQNRMGPPPQGQGKLPPGMNVPPGPGTPGTPGTPGTPGGPPPPPGMYPYPPPGHPGHPPPPPGMFPNGFPPPPPGVAPPNFQHGPPQGPPGGQMQPPPQFGNRFMPPQFMGMPLPPHLQQQQPQQQQQQQQQQQQNQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV37</accession>
    <name>G3AV37_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNL-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_63731</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377877.1">
        <property type="nucleotide sequence ID" value="XM_007377815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV37"/>
    <dbReference type="FunCoup" id="G3AV37">
        <property type="interactions" value="104"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV37"/>
    <dbReference type="GeneID" id="18874885"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63731"/>
    <dbReference type="eggNOG" id="KOG3277">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093902_1_1_1"/>
    <dbReference type="InParanoid" id="G3AV37"/>
    <dbReference type="OrthoDB" id="512667at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024158">
        <property type="entry name" value="Mt_import_TIM15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007853">
        <property type="entry name" value="Znf_DNL-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20922">
        <property type="entry name" value="DNL-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20922:SF13">
        <property type="entry name" value="DNL-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05180">
        <property type="entry name" value="zf-DNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51501">
        <property type="entry name" value="ZF_DNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNL-type" evidence="6">
        <location>
            <begin position="63"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="34"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="167" mass="18508" checksum="D0C16D32410AF6E4" modified="2011-11-16" version="1">MLARFATRAVRVAVPQVTRSVRIGSSIALTRRWYTDKPSTPHATPTTTTQQTRGNTETAAEQEPKGEYLIQFTCNPCSTRSTHTFSKLAYHHGTVLIQCPSCKNRHLIADNLGFARDGRINIEQLLAAQGESVGKEKGDLVFEDVPESMRDKLNNVVTGIKVKEPEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV96</accession>
    <name>G3AV96_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">argininosuccinate lyase</fullName>
            <ecNumber evidence="4">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ARG4</name>
        <name evidence="8" type="ORF">SPAPADRAFT_144378</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="4"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377665.1">
        <property type="nucleotide sequence ID" value="XM_007377603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV96"/>
    <dbReference type="FunCoup" id="G3AV96">
        <property type="interactions" value="1115"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV96"/>
    <dbReference type="GeneID" id="18870622"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144378"/>
    <dbReference type="eggNOG" id="KOG1316">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027272_2_1_1"/>
    <dbReference type="InParanoid" id="G3AV96"/>
    <dbReference type="OMA" id="DFAIEFC"/>
    <dbReference type="OrthoDB" id="2561043at2759"/>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.10:FF:000002">
        <property type="entry name" value="Argininosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000001">
        <property type="entry name" value="Argininosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000025">
        <property type="entry name" value="Argininosuccinate lyase chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814:SF1">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="6">
        <location>
            <begin position="13"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Argininosuccinate lyase C-terminal" evidence="7">
        <location>
            <begin position="371"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW29899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="464" mass="51789" checksum="7B8233D9D403FB22" modified="2011-11-16" version="1">MSAAPSENKLWGGRFTGATDPLMDLYNASLPYDKIMYDADLTGTKVYTAGLQKLGLLTQEELTAIHEGLEVIRKEWQEGKFVEKPGDEDIHTANERRLGEIIGKGIAGKVHTGRSRNDQVATDMRIFVRETLLDLSNKLQQFILAIVNRAESEIDVLMPGYTHLQRAQPIRWAHWLSSYGTYFTEDYKRLQEIIARVNQSPLGSGALAGHPYGIDREFLAQGLGFQGVIGNSLTAVSDRDFVVETLFWSTLFMNHISRFAEDLIIYSTAEFGFIKLADAYSTGSSLMPQKKNPDSLELLRGKSGRVFGSLSGFLMSIKSIPSTYNKDMQEDKEPLFDAMTTVEHSILIATGVISTLSINKEKMEQALTMDMLATDLADYLVRKGVPFRETHHISGECVRVAEEQNLSGIDKLTLDQFKQIDARFGDDVVDVFDFEVSVERRDAIGGTAKSAVYKQLESLKKVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVI1</accession>
    <name>G3AVI1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-CAP/cofactor C-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_158132</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TBCC family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377696.1">
        <property type="nucleotide sequence ID" value="XM_007377634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVI1"/>
    <dbReference type="STRING" id="619300.G3AVI1"/>
    <dbReference type="GeneID" id="18871116"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_158132"/>
    <dbReference type="eggNOG" id="ENOG502T1DX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086432_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVI1"/>
    <dbReference type="OMA" id="HQLRLHN"/>
    <dbReference type="OrthoDB" id="194775at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017901">
        <property type="entry name" value="C-CAP_CF_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027684">
        <property type="entry name" value="TBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012945">
        <property type="entry name" value="Tubulin-bd_cofactor_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15139">
        <property type="entry name" value="TUBULIN FOLDING COFACTOR C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15139:SF0">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07986">
        <property type="entry name" value="TBCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51329">
        <property type="entry name" value="C_CAP_COFACTOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-CAP/cofactor C-like" evidence="2">
        <location>
            <begin position="86"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW29930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="263" mass="29333" checksum="A1A3229CC92DF0DB" modified="2011-11-16" version="1">MSVDTGVTSDLRNEIIGCKDKQQLVQLRQQLDSVQLSIDLTASTTFEQKRIKELIEGLYRIIDNKQVQLSMTRFEFVGEPIPPANPRRTAVTLVESNDEQKSSGSAIVKLNSDKYEISDDRYVLIASSVRSIISTASASPPPSIHMKNAQGSICSLASKGPIFIHNVNNSILVLSCHQARLHNIHNSIIIVNVQNNRIIIEDCDGLVVNDIEVDDFNHPTKETANPHYKIMSRDKQENILKTIGDCSVDQLPVVIRECLAKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVW8</accession>
    <name>G3AVW8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">N-glycosylase/DNA lyase</fullName>
            <ecNumber evidence="3">4.2.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_63635</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type-1 OGG1 family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="3"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377779.1">
        <property type="nucleotide sequence ID" value="XM_007377717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVW8"/>
    <dbReference type="FunCoup" id="G3AVW8">
        <property type="interactions" value="603"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVW8"/>
    <dbReference type="GeneID" id="18874842"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63635"/>
    <dbReference type="eggNOG" id="KOG2875">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027543_3_1_1"/>
    <dbReference type="InParanoid" id="G3AVW8"/>
    <dbReference type="OMA" id="GYAQEYL"/>
    <dbReference type="OrthoDB" id="238681at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034039">
        <property type="term" value="F:8-oxo-7,8-dihydroguanine DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000006">
        <property type="entry name" value="N-glycosylase/DNA lyase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1670.10:FF:000005">
        <property type="entry name" value="N-glycosylase/DNA lyase OGG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012904">
        <property type="entry name" value="OGG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052054">
        <property type="entry name" value="Oxidative_DNA_repair_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10242">
        <property type="entry name" value="8-OXOGUANINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10242:SF2">
        <property type="entry name" value="N-GLYCOSYLASE_DNA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07934">
        <property type="entry name" value="OGG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="14">
        <location>
            <begin position="130"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW30013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="344" mass="40143" checksum="D082AE24D35CDA23" modified="2011-11-16" version="1">MTKELLWNKIPIIQTELNLSKVLRCGQTFRWKNINHEWSFTTQDRIILLKQDDQFIHYSHIMKHDTNGTSSQTLDFLHDYFSLDLKLADLYTSWKTSHKKYEKGISPFDAFQGIRIIRQDPWECLISFICSSNNNVKRISKMCDSLCSEFGTYINEYNDYKYYSFPTAETLAQPDVEAKLRDLGFGYRAKYIHQTAMKFTSPDFPEITIENLNSLRSKDYTEAHEFLLQLTGVGPKVADCICLMSLDKHDIVPIDTHVYQIAVRDFKYKGKKELKTMNKEVYHQIRTFFKEIFGDYAGWAQSVLFAADLGDLNNGTNIIDIKKEISKEKAEPVSRSRKRIKVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVX1</accession>
    <name>G3AVX1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_52860</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377782.1">
        <property type="nucleotide sequence ID" value="XM_007377720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVX1"/>
    <dbReference type="STRING" id="619300.G3AVX1"/>
    <dbReference type="GeneID" id="18871728"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52860"/>
    <dbReference type="eggNOG" id="ENOG502S8Y2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1928913_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVX1"/>
    <dbReference type="OMA" id="NHYSESQ"/>
    <dbReference type="OrthoDB" id="4024075at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="131" mass="15320" checksum="BB18E3247DD9E41B" modified="2011-11-16" version="1">MEFRSKLIEIGYIDALGNSFDLKGTELESCFSFKEYKTVADSMEFDAAEELNSNDTNMDVVDSSLNKSVKRKRGCYKYYSQAQKQRFWNMIIEEGNSTYRSGLLNDINLQTAYTWKRNWDQQVVEEINGIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEE8</accession>
    <name>G3AEE8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Trafficking protein particle complex subunit 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_147315</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373148.1">
        <property type="nucleotide sequence ID" value="XM_007373086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEE8"/>
    <dbReference type="FunCoup" id="G3AEE8">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEE8"/>
    <dbReference type="GeneID" id="18870748"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_147315"/>
    <dbReference type="eggNOG" id="KOG1931">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009596_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEE8"/>
    <dbReference type="OMA" id="YEIHANP"/>
    <dbReference type="OrthoDB" id="10256906at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055504">
        <property type="entry name" value="DUF7076"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055505">
        <property type="entry name" value="DUF7077"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055506">
        <property type="entry name" value="DUF7078"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056916">
        <property type="entry name" value="NTS_TR130"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045126">
        <property type="entry name" value="TRAPPC10/Trs130"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022233">
        <property type="entry name" value="TRAPPC10/Trs130_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056913">
        <property type="entry name" value="TRAPPC10/Trs130_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13251">
        <property type="entry name" value="EPILEPSY HOLOPROSENCEPHALY CANDIDATE 1/TMEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13251:SF3">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23273">
        <property type="entry name" value="DUF7076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23274">
        <property type="entry name" value="DUF7077"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23285">
        <property type="entry name" value="DUF7078"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24967">
        <property type="entry name" value="NTS_TR130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12584">
        <property type="entry name" value="TRAPPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23036">
        <property type="entry name" value="TRAPPC10_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="TRAPPC10/Trs130 N-terminal" evidence="5">
        <location>
            <begin position="12"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Trs130 NTS" evidence="9">
        <location>
            <begin position="334"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7076" evidence="6">
        <location>
            <begin position="583"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7077" evidence="7">
        <location>
            <begin position="737"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7078" evidence="8">
        <location>
            <begin position="935"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="domain" description="TRAPPC10/Trs130 C-terminal" evidence="4">
        <location>
            <begin position="1039"/>
            <end position="1169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1179" mass="136033" checksum="A15D018184BCA995" modified="2011-11-16" version="1">MSQPRPPGPKSPIKIGYYDPFNIYPKIKDEFEKKLPLTSLHLKFQDSQSYKSIPSLPVSMFEEVPKKIETGAGELAFDNVYTRLMFIKIDSIENYRSSVRPLIREWLKNIVFKARSSWMIVLYVPSNVKDTKSVMKVSRFDKLKNDFGIDGKELPVILPGNFPDYDGERCLKYRESGINSKGIDSSQELVLEIKTLLLSSFTTRYGLFRDWIKDHKIHSIEGFISSFRIAELLNDMALYQESLDEIEHVREKLTSVVEYNPNMFDENVHLPKHFEEYTQFESSFADTGIRNRLFRKTDINLFRLHCLLFINYSSVLQALSSSESLSISSIHVSRLYQQLIVFLSNIAGKFPHVDLKEFQYSVIEYYLNIPSINQLIESSKPENEDSINTTLRNIYEFRAELKLFQRSIVVKIAAERGYSISGLKVLEDVPLEDTPAVSKPKKFINPKLTQYMADKSAFFAAFTSITEEIIRDFVECERSKSVDILSIDLAVLNYEKGNYEECLEILRGSYDFFIANGWNYLGGILLEIYNGCIEKLNRNYDEEILTTCLKLATCLVDSTNQTGINSYGLIKNKSQVNKLFSKINEYSLKQEAQIDCSLDELFRTRIYLSLHADASTSRDEYYIELEINNPFQREFHFRSVEAVMVSNEGHQMIFKSENVDIPDSSKHIMKLYSNTFIMGSFKPYKLSIALNEKLSLVSEYIKVHKDGTIFKDSEQSRPTLETKQWYHGSLHTFHDIDKFRCEITCPDKLQLGVSKVLLNIHNGDNEVKNVKIKMFSTTEGLKITQPTFNIDKLDARKTEGVVIPYSYLSQNKVINMKSRIIYEVEGSTYIHEVSYNIDTALTISVSVYDIFKQDFIYSKFQIGTSNPEYPIRVLRNQLTTTNENYIIATSKPNSRELVAFGEQPTCIFFRITPKENYMITSEDTVDLSIWYTNLQEECESHLFDGVVNKLREINLVRYWFMLKSLIFSQARFDLNKYALDDTIEVYNQNELNILAEKVLAEYVEEPADRKELLQLVQDRLTSTHSGTTTRKVIELNISVPIPIIKYLQIVEYEFERKSQYVVGEAIAVTLKVTTISKWSAEVPSKSLPSDEFQALVQNDDNWLVSGYKKHSFTNENSLQTELLLIPLNVGKILLPKVFVKFLNPEHNDSAEHASLEVDFKNGLETLLVVPEVNSVTFSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEL1</accession>
    <name>G3AEL1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Choline/carnitine acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_69177</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372185.1">
        <property type="nucleotide sequence ID" value="XM_007372123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEL1"/>
    <dbReference type="FunCoup" id="G3AEL1">
        <property type="interactions" value="483"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEL1"/>
    <dbReference type="GeneID" id="18875260"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_69177"/>
    <dbReference type="eggNOG" id="KOG3719">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013513_4_0_1"/>
    <dbReference type="InParanoid" id="G3AEL1"/>
    <dbReference type="OMA" id="HILVMRR"/>
    <dbReference type="OrthoDB" id="240216at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004092">
        <property type="term" value="F:carnitine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000019">
        <property type="entry name" value="Carnitine acetyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000003">
        <property type="entry name" value="Carnitine acetyl transferase FacC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF29">
        <property type="entry name" value="MITOCHONDRIAL CARNITINE O-ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00440">
        <property type="entry name" value="ACYLTRANSF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="6">
        <location>
            <begin position="12"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="739" mass="83571" checksum="3C72186231D598D9" modified="2011-11-16" version="1">MTYQHQDTLQKLPIPDLNQTCTNYLKVLKPLQTEAEHQQTVEAVESFLKSGVGPYLDSELRAFAKNRASYIEQFWYDAYLNYDSPVVLNLNPFFLLEDDPFTNENSPFDPQVKRATSLTLSSLKFIQALKNETLSVDTVRGGKPMCMYQYTKLFGASRIPTQEGCQMQSDPSSNHIVVMSKSQFYWFDVLDSKNNVILSESELNINFKSIIYDSLHTPLNEIAKSSFGVLTTENRRIWAHVRNDVISHNQINSEILSIIDSALFVLCLDDIAINDLSELSKNMLCGLSILDKGVQIGTCTNRWYDKLQIIITKNGKAGINFEHTGVDGHTVLRFVSDIYTDSILRFANTINNNAPSLWNTTTNFEYEEEDLITVPRKLEWDLTPELSLTLRFGETRLSDLINQNEFRHLEFENYGSTQIKKMKFSPDAFVQMAFQATYYALYGKVECTYEPAMTKQFFHGRTEAIRTVSQESNLFVRKFFDASVSAQEKLDYLDAACKAHSAQTRLCSQGQGVDRHLYALFCLWRRYLAEIEDEENTQNGGAAENDNMSDDTTFVATHHPKDLNDRLRNIPDLFADSGWDKLNNTIISTSNCGNPSLRLFGFGPVSANGFGLGYIIKDDSISICASSKHRQTQRFLTTLKSYLLEIRNMHRELKQRKKVAAAVAAEAAAKAIPVIVEPSPSHLNSLLGGYGYFDMGEDDIKSMGEVPEPPFLNRQASGSSPGVGFSIREIGKKLRLSEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AER9</accession>
    <name>G3AER9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycogen debranching enzyme</fullName>
            <ecNumber evidence="5">2.4.1.25</ecNumber>
            <ecNumber evidence="6">3.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Glycogen debrancher</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">SPAPADRAFT_132625</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Hydrolysis of (1-&gt;6)-alpha-D-glucosidic branch linkages in glycogen phosphorylase limit dextrin.</text>
            <dbReference type="EC" id="3.2.1.33"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1-&gt;4)-alpha-D-glucan.</text>
            <dbReference type="EC" id="2.4.1.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the glycogen debranching enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.25" evidence="5"/>
    <dbReference type="EC" id="3.2.1.33" evidence="6"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373107.1">
        <property type="nucleotide sequence ID" value="XM_007373045.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AER9">
        <property type="interactions" value="429"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AER9"/>
    <dbReference type="GeneID" id="18869674"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132625"/>
    <dbReference type="eggNOG" id="KOG3625">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001517_2_0_1"/>
    <dbReference type="InParanoid" id="G3AER9"/>
    <dbReference type="OMA" id="YEEGHVH"/>
    <dbReference type="OrthoDB" id="10248904at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004134">
        <property type="term" value="F:4-alpha-glucanotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004135">
        <property type="term" value="F:amylo-alpha-1,6-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11327">
        <property type="entry name" value="AmyAc_Glg_debranch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000070">
        <property type="entry name" value="GDB1p Glycogen debranching enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000039">
        <property type="entry name" value="Glycogen debranching enzyme Gdb1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000242">
        <property type="entry name" value="Glycogen debranching enzyme Gdb1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010401">
        <property type="entry name" value="AGL/Gdb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032788">
        <property type="entry name" value="AGL_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029436">
        <property type="entry name" value="AGL_euk_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032792">
        <property type="entry name" value="AGL_glucanoTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032790">
        <property type="entry name" value="GDE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006421">
        <property type="entry name" value="Glycogen_debranch_met"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01531">
        <property type="entry name" value="glyc_debranch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10569">
        <property type="entry name" value="GLYCOGEN DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10569:SF2">
        <property type="entry name" value="GLYCOGEN DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06202">
        <property type="entry name" value="GDE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14701">
        <property type="entry name" value="hDGE_amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14702">
        <property type="entry name" value="hGDE_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14699">
        <property type="entry name" value="hGDE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Eukaryotic glycogen debranching enzyme N-terminal" evidence="18">
        <location>
            <begin position="51"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Glycogen debranching enzyme glucanotransferase" evidence="19">
        <location>
            <begin position="143"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="Glycogen debranching enzyme central" evidence="20">
        <location>
            <begin position="725"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="domain" description="Glycogen debranching enzyme C-terminal" evidence="17">
        <location>
            <begin position="1038"/>
            <end position="1512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF06202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF14699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF14701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF14702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EGW35695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1530" mass="174714" checksum="81A97FFF4570437C" modified="2011-11-16" version="1">MTSSSTERSILFRLSNLGEPVNNENVNNGVLILPSVQLPKDFRKGDVLFELHFQLNAASRVSRDGVFHTNVPQSFDQPFDRYTHFDYPIKSSFHQDISIPIPIYRPGAYNYYITYTDEHNKQKQTEKFYFNVPPNLTIDNAFVAFNSINIETVISKWIGPLENWDKFFGKISDKGYNMIHFTPLQHRGESDSPYSIYDQLQYDPNIFPDSDKAPAQIQSILQKHKLLALTDVVWNHTANNSDWLKEHPESGYNAESSPHLAAAIELDGALLEFSDNLEELGLPVDIKTESDLDEIIHGITSHVLDKLKLWQYYVFNSKQTLIELEQTYNEGLDKVAPIEIPHDVDIHSLKSLSKYTLEVTNIHPTTIIADRFSNKFDIKKFLAILYAIYDTDTEFEVVKSKAGDIIDAINVDLYALYDSDMSSIKSQVADRIRYLRLADNGPKQGPVTKKNPLTEPYFTRFVDNKGRKQALANNGWIWGGNPLVDFASNKSRAYLRREVIVWGDCVKLRYGEKYEDSPALWDRMIKYTQEAAKTFTGFRIDNCHSTPLHVGERLLDEARKVNPDLYVIAELFSGSEEMDKIFVERLGINSLIREAMQPWDVQELSRLVHRHGGRPIGSLTWLPLDDFTYPAEAEPIKDKYLNAYTELEIPKALTSQAPHALFMDCTHDNEMPAQKRTVEDTLPNAALVAFCSSAIGSVYGYDECYPKILNLVHEHRQYSLDDDNGIGKVKAKLHKIRKDLALESEDILRDHEMYIHHEGQYITIQRYNARTGKGWFLIARTKFSGSFQKQILSPVVLAGANVKHEFSYTLKKTGEYEENDKFLAGIPVEVVDLEPPKLTIDEKETIIRVDESFIPGSISVFSTEIPGLNVSLDNYVKEGAIEASRDLDLYDLNALLYKSSPEELDASSGQEDVYRIPNYGTLVYAGLEGWNTALKHVIWSNNLGHAICNHLREGEWALDYIVNRLDKYVAHSAKLGKFQEWLRSRFDAVKKSAPYFLRPHYFALIVGIAYEAARFRAIRSFDKTIQQATNFVQSLALTSVQMVGYMQNTSLYPLVQVPSMAAGLPHFSNSYMRCWGRDVFIAFRGLLLVPGRYKDAKTHILGFAMTLKHGLIPNLLDAGRNPRYNARDAAWFFLQAVQEYVNYAPDGLSILDEKVKRRFPLDDTYIPHTDPRAFSHESTIREIIYEILSRHAAGIKYREANAGPNLDSQMKDEGFNVEVFIDWETGLVHGGSQFNCGTWMDKMGESERAHNKGVPGTPRDGAAIELQGLMKSALRFVNKLHDKGKFEYTHVKKADGSEISLKDWEQLIQDNFEKCFYIPTSSKDDHLYSLDPSLVHRRGIYKDLYKSGKPYEDYQLRPNFSIAMCAAPELFTPLKALTALRNADKIIRGPVGMRTLDPSDWNYHPYYNNSEDSDNFATSKGRNYHQGPEWVWNLGYFLRAFLHFHVEAVADSHTQQGDPSINILTELNERLQGNIKWIKNSPWAGLTELTNKDGVICNDSSPTQAWSGSCLLDLYHDVWTDERYRSKYCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEV7</accession>
    <name>G3AEV7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_53945</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the APC13 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373199.1">
        <property type="nucleotide sequence ID" value="XM_007373137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEV7"/>
    <dbReference type="GeneID" id="18871824"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53945"/>
    <dbReference type="eggNOG" id="ENOG502T0MK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1299536_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEV7"/>
    <dbReference type="OMA" id="ERWKDEP"/>
    <dbReference type="OrthoDB" id="4023481at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008401">
        <property type="entry name" value="Apc13"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05839">
        <property type="entry name" value="Apc13p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="173"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="200"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="227" mass="25903" checksum="F8956B773E78750B" modified="2011-11-16" version="1">MSRDGVYSYIHLDDSKKVLYMEQWKQESLPFDDIETHNLVEATSTLFTKLFLDDEEEEESLPIHPAVFGNKIYKSRSMLKEKVWTDLALTEKFLDESSELNKLHDKTILPSLSSEVEQQFGELSLAEFLSNGYNIPDDHLDSVLSPNLTDSIPQHHFRRHRLSHRDISLNSTSDLSVPNNSFQNIRSSSGTGNPVAPPGSLQTPRTGRVRQVSTDKNFQTPISRIMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEY0</accession>
    <name>G3AEY0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA-directed RNA polymerase, mitochondrial</fullName>
            <ecNumber evidence="3">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_47865</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 16">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phage and mitochondrial RNA polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="3"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372196.1">
        <property type="nucleotide sequence ID" value="XM_007372134.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AEY0">
        <property type="interactions" value="257"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEY0"/>
    <dbReference type="GeneID" id="18871154"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_47865"/>
    <dbReference type="eggNOG" id="KOG1038">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003364_1_0_1"/>
    <dbReference type="InParanoid" id="G3AEY0"/>
    <dbReference type="OMA" id="KWFEVDM"/>
    <dbReference type="OrthoDB" id="276422at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034245">
        <property type="term" value="C:mitochondrial DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001018">
        <property type="term" value="F:mitochondrial promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006391">
        <property type="term" value="P:transcription initiation at mitochondrial promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000041">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.280:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1320.10">
        <property type="entry name" value="DNA-directed RNA polymerase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024075">
        <property type="entry name" value="DNA-dir_RNA_pol_helix_hairp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046950">
        <property type="entry name" value="DNA-dir_Rpol_C_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002092">
        <property type="entry name" value="DNA-dir_Rpol_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037159">
        <property type="entry name" value="RNA_POL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029262">
        <property type="entry name" value="RPOL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00940">
        <property type="entry name" value="RNA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14700">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01311">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00489">
        <property type="entry name" value="RNA_POL_PHAGE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase N-terminal" evidence="13">
        <location>
            <begin position="371"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00073509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1287" mass="147882" checksum="2D18B887B30B2EF7" modified="2011-11-16" version="1">MLRTSLKSSSYRRSRALVQRLLPQIQYAYINTRSESKTLAQSAVAVSPVSRLLEPLKSERTESNAKHSISKLTQKSFSEIEQTEFTAPAAERQPGYYSSKGDNVIRQPFAREINYLKSLMESFVSDQYYEKAYPLLKTLSGLVTPEEFTKFANIYLEALVQDEKVSVRELEQVIDKMQRECHFESNNRTRALRLTKYLETDPKFDLDEFFRIPKIEDIRSTLSNVDIIGVDNLYTIFDHPKAQTWIPSELKESYHEYDLNKNKEIVEDTKKLQEKDGSIRSLEKDDLSELEAVDSFGLQIVRHSLLGLEASPDKIAVFTSDIKEIIKDMTNEESKQKITSGKLNYFEMYQLLETQEQRDQFNHALANFNLDRQKQMEIRGVEGAKEKWKHEYEETKKRDSIMSTKNLNAQLYEWYQKLVPLLQKERQECLDLIDDKATNQKSSENKERAVYAPFFVKIDPEKAAMTTILEVMKLQSTGGISNGMRALTALQSVGKALEYDYITQKLSKKDLKRTQTSKHKGSRLLLNLVRNAAEGDPNEDFGWSQDISIRLGAVFVSFLMEVAKVRVSRHTVETEQSAFFHSIQYIGGQRIGAIKLHSDLVKSINRSSLDSCVQPQHMPMPVKPKDWDGYYGRGQVMSPLVRVRDSPETAAYVKTAARNGNLDQVYAGLNVLGNTAWTVNKDIFKVISHYWNTGKEFLDIPGVLDKPILPPKPERSDDPSIKYKYRKLVTQLKSEFSTMRSQRCDANYKLEIARAFIGEKLYFPHNLDFRGRAYPISPHFNHLGGDMTRSLFLFWDGKELGERGLRWLKIQLANLYGFDKAPFDERVKFVEDNLQNVFESAKNPYSEDAWWKKADKPWQALGVCFDLRRAYKLPDPTKYVSHMAVHQDGTCNGLQHYAALGGDLEGAQQVNLARADRPQDVYAYVAGIVRELLEKDAQKNNKYALFLKDKINRKVVKPTVMTNVYGVTYIGALAQVRKQLEDYFPDNQRDSLQEHARYLTTHVFAAIRQLFENAHLIQDWLGESARRISKSVSIDYKESDNVALHSSAVIWTTPLNLPCVQPYRKGTSESLKTAVQTLTINRIDGAGMVDGRKQVAGFPPNFVHSLDATHMMMTAAACGERGLKFASVHDSFWTHAADVDTMNEQIREQFVKLHSSNLISLLKEEFEIRYKDNYQLIKISSKHPLVKSIKELKQQWTKQLNRPITLGDELYMERTRLEFLNSDDPQKIKAAQDMVTTIKLAHEYEGEIEDVNDRGTYEILIPLKFPEVPPKGEFDVNRVKDSLYFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEY2</accession>
    <name>G3AEY2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_145250</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372198.1">
        <property type="nucleotide sequence ID" value="XM_007372136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEY2"/>
    <dbReference type="FunCoup" id="G3AEY2">
        <property type="interactions" value="773"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEY2"/>
    <dbReference type="GeneID" id="18870679"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_145250"/>
    <dbReference type="eggNOG" id="KOG0812">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044998_0_1_1"/>
    <dbReference type="InParanoid" id="G3AEY2"/>
    <dbReference type="OMA" id="EHNHNVV"/>
    <dbReference type="OrthoDB" id="421009at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090083">
        <property type="term" value="P:regulation of inclusion body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd15844">
        <property type="entry name" value="SNARE_syntaxin5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021538">
        <property type="entry name" value="Syntaxin-5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF3">
        <property type="entry name" value="SYNTAXIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11416">
        <property type="entry name" value="Syntaxin-5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="10">
        <location>
            <begin position="239"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="165"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="202"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="330" mass="37393" checksum="BD305B7EB502C914" modified="2011-11-16" version="1">MSVSIQNRTIEFQQCVSTYDKINKKQRHAQGLGGTPSSNIPPKKSQFSQQASIIAKDISHVTELLSKLALLAKRKPIFDDKPIEIGELTYVIKQEIFKIETNIQNLQKFTKGDHSIQIDSQISQYSKNVLNLLNSKMKNISGEFKNVLEIRQRNEIANKNRTENFLSSSVSSRGASPMLHNENPFASSSSLNNSPFDPDKAITSSTDTDLVSSPYGNSGEYLTLPKQTQQMLLMEEQSTQYLQQRNRAVETIESTINEVGNLFQQLATMVSEQGEQIQRIDENVEDISLNISGAQRELLKYYANITSNRWLFLKIFGVLIIFFFIWVLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF27</accession>
    <name>G3AF27_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Serine/threonine protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SKS1</name>
        <name evidence="8" type="ORF">SPAPADRAFT_57926</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_007372181.1"/>
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        <property type="interactions" value="263"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF27"/>
    <dbReference type="GeneID" id="18872249"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57926"/>
    <dbReference type="eggNOG" id="KOG0583">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_172_4_1"/>
    <dbReference type="InParanoid" id="G3AF27"/>
    <dbReference type="OMA" id="ICCNACR"/>
    <dbReference type="OrthoDB" id="541276at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
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        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF150">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE STK11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="52"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="524" mass="61133" checksum="FA80A3DA2D447FCC" modified="2011-11-16" version="1">MTLDVLSFQDPHPAMHQQQQYITSQQQVKRQQHSQRPNPNMVLDGYMLSPELRFIKKIGAGTYGLIYLVENITTGRRYAAKMLLKQPPFKQGDPKDSSGNKKIIQQHFYQYFLTNPMPEPTPVNLEYIRQDQSHQYQFMTEIQLHLKVHHHPNVITIHQVYELQEFAIVILLDYFEQGDLFGNIIDRQIFQNHTGDKQLLMKNAMLQLIDAVEYCHQQSVYHCDLKPENIMIHYNPNYKRPDNSCIIDYNELHLVLIDFGLAMSSDLICCNACRGSSFYMAPERTTNFNTHRVVRNLVDLSQYHSIEINNTTVNESNSKYLPTLAGDIWSLGVLFINITCSRNPWPIASFNNSHNDVFKSYILNNNKDILASILPISKQFNRLLDRIFQLNPNDRISLPQLYQEVIKCNFFKDTPSHHANTAPTTTTIHARRHTVVPESIPKPTHKVNEQLYTPPETTRYNSYVSDEEENIDDDAYEYQEPHQEPELQNHKYYYQEQQPPASQIYAHVPKFNLETPSNSVVYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF90</accession>
    <name>G3AF90_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LicD/FKTN/FKRP nucleotidyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_58005</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007372229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF90"/>
    <dbReference type="STRING" id="619300.G3AF90"/>
    <dbReference type="GeneID" id="18872278"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58005"/>
    <dbReference type="eggNOG" id="ENOG502QREF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_689089_0_0_1"/>
    <dbReference type="InParanoid" id="G3AF90"/>
    <dbReference type="OrthoDB" id="444255at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009644">
        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF28">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE TRANSFERASE FKTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="5">
        <location>
            <begin position="151"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="5">
        <location>
            <begin position="278"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="469" mass="55792" checksum="BFE47A81654D0C72" modified="2011-11-16" version="1">MVAMIKQTDPTKPRDLYLPESAFNYQQDKIDVQIAQYESRMSQLRDLTTNELSYDQSKINAIRFTRNEKIYFDGLKFANKYQLNNEPTYFRMARLNFGNEENESDAGWHYEWRFFNGALRYLKKGWNQEELLIREKVLLDRILRNWFRFANEKGIVSWISHGPLLSWYWDGLLFPFDEDIDIQMPAEELSRFSKLYNQTLVVEDITEGFGKYFIDCSTFIHHRGKSYKENHIDARFIDIDTGSYIDITGLGISDEKYPDKYKELVESAEREGRAKPVYNCRNLHFYSYDELSPLRFTMMGGVPLYVPNRIEDILNDEYSKGLTSYEYGGFYFVDAINLWIHHSKLEFLFPYETFKTSDGKINELRFTQLVQGIGEKEVVKLLENDEDILLEYYLTKNVTDIHKEELTYLFNTPNGSNTKLKDVGHQKTNGEIADDKEYHKFTSKFKFQKPFRRPLFNYEMIDKPRHHQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFC7</accession>
    <name>G3AFC7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_58038</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372329.1">
        <property type="nucleotide sequence ID" value="XM_007372267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFC7"/>
    <dbReference type="STRING" id="619300.G3AFC7"/>
    <dbReference type="GeneID" id="18872296"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58038"/>
    <dbReference type="eggNOG" id="KOG1657">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084533_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFC7"/>
    <dbReference type="OMA" id="HEGENDQ"/>
    <dbReference type="OrthoDB" id="6781668at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008623">
        <property type="term" value="C:CHRAC"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23645">
        <property type="entry name" value="HFD_Dpb3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF151">
        <property type="entry name" value="DNA POLYMERASE EPSILON NONCATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="4">
        <location>
            <begin position="76"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="179"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="223" mass="24158" checksum="3E564417569BE671" modified="2011-11-16" version="1" fragment="single">MSSINEIDESRQTTVSPGNTPETSLPAEKSTTPPATDAMETDDPEGTETPAVDGENEPTSTAEAEIETEEATEQSMSLPLSKIKRIFKMDPEYLAASQTAVYTAGLATELFVQYFVEQASLQAKMDKRKKIQYKDFSSAVASHDSLNFLSDTIPKTQPIGELIQNKKVNIPGSAPGKVTNSTGNSSDNTSRPAEILSKGQQTLHFPIATTPKEDKPIRKAVIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFG1</accession>
    <name>G3AFG1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DUF159-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_145639</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SOS response-associated peptidase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372362.1">
        <property type="nucleotide sequence ID" value="XM_007372300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFG1"/>
    <dbReference type="FunCoup" id="G3AFG1">
        <property type="interactions" value="616"/>
    </dbReference>
    <dbReference type="GeneID" id="18870693"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_145639"/>
    <dbReference type="eggNOG" id="KOG2618">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035990_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFG1"/>
    <dbReference type="OMA" id="SYNKGPQ"/>
    <dbReference type="OrthoDB" id="2111841at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106300">
        <property type="term" value="P:protein-DNA covalent cross-linking repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1680.10">
        <property type="entry name" value="SOS response associated peptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003738">
        <property type="entry name" value="SRAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036590">
        <property type="entry name" value="SRAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604:SF0">
        <property type="entry name" value="ABASIC SITE PROCESSING PROTEIN HMCES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604">
        <property type="entry name" value="DC12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02586">
        <property type="entry name" value="SRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143081">
        <property type="entry name" value="BB1717-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0190">Covalent protein-DNA linkage</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="252"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="255"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="268"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="308"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="332"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="44138" checksum="291516D7F306C8F5" modified="2011-11-16" version="1" fragment="single">MARVELDATRVQDWYPSYNIAPTNSALIVYKVQSQDSGINQKYVLEPSKFGLLPSWARPKDPTPVNKGTPNEGPQYSREIQRYQGKYFNCRRESLQEHQPQWNASRKHRCVVPIQGYFEWLKTKGDKIPYYVHSKKSALVYLAGFYSHNSNYENYRNNSDFLSSFTIITAPALKTDTHDISWLHDRKPVFLAPGSKEWDDWLDPSKNWEDSMIETCLNSAANKAYADIEGYPVSKKVGVPSNNGEDVIERVESKPQASISSFFQPQKKQPELKVEPTDEKIKQEDDNSKNDDVDVKEEDYLDGLDAPEINEGESEEEEPVKQETEQDEKVEEDAVGKEEREEDAAKGDPEAKDEVEQEVKADEERLEQEAAEKDKEAAKEEEGDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFI7</accession>
    <name>G3AFI7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA-splicing endonuclease subunit Sen15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_58100</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEN15 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372388.1">
        <property type="nucleotide sequence ID" value="XM_007372326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFI7"/>
    <dbReference type="FunCoup" id="G3AFI7">
        <property type="interactions" value="83"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFI7"/>
    <dbReference type="GeneID" id="18872323"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58100"/>
    <dbReference type="eggNOG" id="ENOG502SC4F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083361_2_0_1"/>
    <dbReference type="InParanoid" id="G3AFI7"/>
    <dbReference type="OMA" id="VYYFVYK"/>
    <dbReference type="OrthoDB" id="10002170at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000214">
        <property type="term" value="C:tRNA-intron endonuclease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000379">
        <property type="term" value="P:tRNA-type intron splice site recognition and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042777">
        <property type="entry name" value="Sen15_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018593">
        <property type="entry name" value="tRNA-endonuc_su_Sen15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28518">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28518:SF1">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09631">
        <property type="entry name" value="Sen15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA-splicing endonuclease subunit Sen15" evidence="3">
        <location>
            <begin position="8"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="111" mass="12666" checksum="8A806E8665514450" modified="2011-11-16" version="1">MTSTLAEQVKINLTHYNQWSDVVIHAVSNELFAISGKPPQKLDDKEIDVEWIVPKTNSNSKLSVDEINQWFKRIAEIHGNPPQRIAIAVVNDDGTIVYYFIHEGIVKPRQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFQ4</accession>
    <name>G3AFQ4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycine zipper 2TM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_58175</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372455.1">
        <property type="nucleotide sequence ID" value="XM_007372393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFQ4"/>
    <dbReference type="STRING" id="619300.G3AFQ4"/>
    <dbReference type="GeneID" id="18872358"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58175"/>
    <dbReference type="eggNOG" id="ENOG502S756">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2086292_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFQ4"/>
    <dbReference type="OMA" id="YEDYRDD"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008816">
        <property type="entry name" value="Gly_zipper_2TM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37014">
        <property type="entry name" value="EXPRESSION LETHALITY PROTEIN HEL10, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G06580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37014:SF10">
        <property type="entry name" value="RICH PROTEIN MS8, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G05650)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05433">
        <property type="entry name" value="Rick_17kDa_Anti"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycine zipper 2TM" evidence="2">
        <location>
            <begin position="36"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="117" mass="12576" checksum="060D7511C873545B" modified="2011-11-16" version="1">MSANEFYSSGDQGSYQPNKANQSNDDGQQGDRGLGATLLGGAAGGFGGSKLGGSEHGKLGAVIGAIGGAIAANKAEDAYEDYRDDRKQQQQYGSSGRRDDHYDHHNDNYRRDDDRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG11</accession>
    <name>G3AG11_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Chorismate mutase</fullName>
            <ecNumber evidence="4 15">5.4.99.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_58336</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the Claisen rearrangement of chorismate to prephenate. Acts at the first branch point in the aromatic amino acid pathway where it steers biosynthesis towards phenylalanine and tyrosine, and away from tryptophan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>chorismate = prephenate</text>
            <dbReference type="Rhea" id="RHEA:13897"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="EC" id="5.4.99.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:13898"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="5.4.99.5" evidence="4 15"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372562.1">
        <property type="nucleotide sequence ID" value="XM_007372500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG11"/>
    <dbReference type="FunCoup" id="G3AG11">
        <property type="interactions" value="170"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG11"/>
    <dbReference type="GeneID" id="18872411"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58336"/>
    <dbReference type="eggNOG" id="KOG0795">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057757_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG11"/>
    <dbReference type="OMA" id="FLDWALM"/>
    <dbReference type="OrthoDB" id="191918at2759"/>
    <dbReference type="UniPathway" id="UPA00120">
        <property type="reaction ID" value="UER00203"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072545">
        <property type="term" value="F:L-tyrosine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120284">
        <property type="term" value="F:tryptophan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.590.10:FF:000002">
        <property type="entry name" value="Chorismate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.590.10">
        <property type="entry name" value="Chorismate mutase, AroQ class superfamily, eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008238">
        <property type="entry name" value="Chorismate_mutase_AroQ_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037039">
        <property type="entry name" value="CM_AroQ_sf_eucaryotic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01802">
        <property type="entry name" value="CM_pl-yst"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21145">
        <property type="entry name" value="CHORISMATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21145:SF12">
        <property type="entry name" value="CHORISMATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017318">
        <property type="entry name" value="Chor_mut_AroQ_eu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="Chorismate mutase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51169">
        <property type="entry name" value="CHORISMATE_MUT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="9 15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0827">Tyrosine biosynthesis</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="16">
        <location>
            <begin position="140"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR017318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW35150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="268" mass="30989" checksum="836A01DE703A0775" modified="2011-11-16" version="1">MDFMKPETVLDLANIRQALVRMEDTIVFDLIERSQFYSSPSVYEKNKFNIPNFDGTFLDWALQQMEIAHSQIRRYEAPDEIPFFPDIILPPILPPINYPKILANYSDEINVNAEIMEFYVKEIVPQVSCKVGDQTENLGSVSTCDIETLQAISRRIHFGKFVAEAKYQSDKPGYIKLILNKDVKGIEDSITNSAVEQKILERLVTKGESYGTDPSLKYSQKPQSKVDPKVIAKIYKDWIIPLTKKVEIDYLLRRLEDEEPELVEKYRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGE5</accession>
    <name>G3AGE5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Agglutinin-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_64434</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ALS family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372696.1">
        <property type="nucleotide sequence ID" value="XM_007372634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGE5"/>
    <dbReference type="GeneID" id="18874974"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64434"/>
    <dbReference type="HOGENOM" id="CLU_322927_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGE5"/>
    <dbReference type="OMA" id="CNSENEA"/>
    <dbReference type="OrthoDB" id="3981162at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044403">
        <property type="term" value="P:biological process involved in symbiotic interaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2430">
        <property type="entry name" value="Agglutinin-like protein, N-terminal domain, N2 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008966">
        <property type="entry name" value="Adhesion_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008440">
        <property type="entry name" value="Agglutinin-like_ALS_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024672">
        <property type="entry name" value="Agglutinin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043063">
        <property type="entry name" value="Agglutinin-like_N_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033504">
        <property type="entry name" value="ALS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011252">
        <property type="entry name" value="Fibrogen-bd_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793:SF2">
        <property type="entry name" value="AGGLUTININ-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793">
        <property type="entry name" value="ALPHA-AGGLUTININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05792">
        <property type="entry name" value="Candida_ALS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11766">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01056">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49401">
        <property type="entry name" value="Bacterial adhesins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0134">Cell wall</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 17" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0843">Virulence</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442356" description="Agglutinin-like protein N-terminal domain-containing protein" evidence="17">
        <location>
            <begin position="17"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="domain" description="Agglutinin-like protein N-terminal" evidence="18">
        <location>
            <begin position="52"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="435"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="745"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="449"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="468"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="542"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="577"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="748"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="785"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="839"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW35284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="896" mass="97343" checksum="89E77F2745770E41" modified="2011-11-16" version="1">MYLLLILLQLLTFAATQEISGVFTSFNSLTWLNSNPNTFQTPASPAWIAELSWKIDGSVVSAGDTFSLTMPCVFRFYTDQDSVNLRVGSTNYATCDFITGGISSTDSELNCVLSDEVEESILAVGKLTFPIVFNIGRSTEDVDLYCSTFYQGGLNTISFMDGDNTLSIDVEFQDGTPSGDPNGPMYGARMLPSSNRWQNYVLGGLCPGGLSTATIGITINDDVSTIDCETANIRLTNAVNDWFYPESFKTFTTASKLCRSNSFMITYWGVPDGYRVFIEAVINTDGNPPRVQYTNKYLCPTTIDNGVWVNWLPYDSVEADGPGKEIITVTETWTGSDTETITKSFDEDEDLTITIAVNIPIPTITLTETYVGITTSYTTYTVEPGETASVVVYDPVHTTITEESCWESDESITTTIIDPDWASDTILIVTPCEVTSTEQEESSTKVEDIPSDDTLDDDSYNPPEIADNASSEEITSGEVQSTEILSSEEVQSTEILSSEEVQSTEILSSEESFSQDSTTVPYGSTKETEISTEIVLEETFSTEDTTPENNTWDGLTDDDSQQPSSNTKNDSNEEYTSDQIDISSSGSETSAKQTTTETIPDNHTTDIVQEDPGTEILSVENTTVRVPDDNTTDISPSDVICNSENEASSEPNETRTYVSIVPTTKSKDSTITSAFSGYEQDTITKVITYTTTICESDTVTQVITYTTIITNHDSIAKTATEMTTEATSFAVTTVGETRTIIYLQDSMGTTNDTGNNSSNTKSDSRTASNNTGNNSSNTKSYSGTASNEEIQTTANSENNSEHTKSESGQSDISEYYSGGTESDSGKGNKSVVPEENQGTNSRNNNSSTNSNNRSDDQTTRGSYSTSLQLVTSNGIGSTRGTSTFSLLFTFVIAILY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGN1</accession>
    <name>G3AGN1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58593</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372782.1">
        <property type="nucleotide sequence ID" value="XM_007372720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGN1"/>
    <dbReference type="STRING" id="619300.G3AGN1"/>
    <dbReference type="GeneID" id="18872521"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58593"/>
    <dbReference type="eggNOG" id="ENOG502QRVJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_742207_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGN1"/>
    <dbReference type="OMA" id="SIMNFAN"/>
    <dbReference type="OrthoDB" id="5426978at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="373" mass="42537" checksum="E36CE2D626217EE6" modified="2011-11-16" version="1">MNDNSLVFWFSTLLIANYSILLNGDVYSMGISWTCSILNDFKVLEKFIEFVEKSQSSDYDNITLYFAKLYYSLDLIDDIYCLGMGTQKSINQNTLIQFLHANIRFFVKEDESIKSGMALFKNAPLLMHMINVRDQYIYEGKYSKPIPIQQQTSDDQFAAHFMTLVSEKYDLINYLLEINHFIVHDSNKHHDSDELYETLMDYNLKLIRLVKKLSSSIMNFANFIGTSTTGSAAGVAATTATTSSPTSPSLKPALVNPLLNISIGQLFKLIKLIKLIIDSLTTSKQSSTDLENRLTKINNDLSISFNLLNLNLVNLQLGTKSSLIIKKKVGDYKFNFNKPTNITTTEQSLNSYIQEFTNTILPFIQQENRHGWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGS5</accession>
    <name>G3AGS5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Crh-like protein</fullName>
            <ecNumber evidence="18">3.2.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">SPAPADRAFT_58626</name>
    </gene>
    <organism evidence="25">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <topology evidence="3">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the glycosyl hydrolase 16 family. CRH1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.2.-.-" evidence="18"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372820.1">
        <property type="nucleotide sequence ID" value="XM_007372758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGS5"/>
    <dbReference type="FunCoup" id="G3AGS5">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGS5"/>
    <dbReference type="GeneID" id="18872540"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58626"/>
    <dbReference type="eggNOG" id="ENOG502QQ71">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027506_2_1_1"/>
    <dbReference type="InParanoid" id="G3AGS5"/>
    <dbReference type="OMA" id="QTPMNIH"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006030">
        <property type="term" value="P:chitin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02183">
        <property type="entry name" value="GH16_fungal_CRH1_transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000162">
        <property type="entry name" value="Glycosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017168">
        <property type="entry name" value="CHR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF68">
        <property type="entry name" value="GLYCOSIDASE CRH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037299">
        <property type="entry name" value="Glycosidase_CRH1_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0134">Cell wall</keyword>
    <keyword evidence="16" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="12 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="10 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 22" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442054" description="Crh-like protein" evidence="22">
        <location>
            <begin position="20"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="23">
        <location>
            <begin position="15"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="297"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="363"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="364"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="19">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="19">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="21"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00038074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR037299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR037299-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR037299-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="EGW35408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="465" mass="48614" checksum="2B10D3D2DC1A9299" modified="2011-11-16" version="1">MKYITLATIATTLVAFVNAACDPLQGTCPADAALGSSFREDFQSGPGPHFVQLDKQGSVDYGDNGVSLTINNRFDNPTLESNFYIMFGKVEVVLQAALGKGIVSSFYLQSDDLDEIDIELFGGDAYEFQSNYFIKGNTATWDRGGYHPTSSSPLENFHTYTIDWSEDSLSWILDGNTVRTIDTSNPQGYPQSPMKIYAGVWAGGDPDNQPGTIEWAGGITDYSQAPFSMHIKSIIVTDYSSGDKYTYSDTSGSWQSIKAENGEVNGRYQQAQADFQSLQNGGSVSAKSVAPAASSTSIATSSVPSSSTVAPQHPTSLTSSASSSSEATTSDPTTSSTSAPSPAVSSSVQTPSSLVFIQTTGELTTSSTPTQTPTQAPTQASVQAPVQSSKPVAFTPNISASPTGAASVVTVGGHSSNFITFISTTNGSPTDTPVATQSSNSANSSNSLKVISILGAIGLISSLMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGV8</accession>
    <name>G3AGV8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_57682</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372043.1">
        <property type="nucleotide sequence ID" value="XM_007371981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGV8"/>
    <dbReference type="STRING" id="619300.G3AGV8"/>
    <dbReference type="GeneID" id="18872148"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57682"/>
    <dbReference type="eggNOG" id="ENOG502RQ9E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1299535_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGV8"/>
    <dbReference type="OMA" id="FFTHEEF"/>
    <dbReference type="OrthoDB" id="4022859at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="225" mass="25904" checksum="00621286414E5113" modified="2011-11-16" version="1">MMATLSPCESCLTLATPVQYNSQVFTNYLNTRLTKTRTVQHLESNDEELELIMIYNHHDINVKLTAVHNLITTYCQIDIADLTLSHLFIYFYATQSTLGFYPQEFLINQINLIHLGKSYTFGLNANQPKLKELNVHTYNKFLIVIKPDTQGGCYKEFKIEKYLNQEKFVDQSTGSVTFADSIASKLKQVGSNHKTISKSSRHSKRIILRRILHNLHRGYSDVAHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGW1</accession>
    <name>G3AGW1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_57685</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Lipid-anchor</topology>
            <topology evidence="1 10">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372046.1">
        <property type="nucleotide sequence ID" value="XM_007371984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGW1"/>
    <dbReference type="FunCoup" id="G3AGW1">
        <property type="interactions" value="171"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGW1"/>
    <dbReference type="GeneID" id="18872151"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57685"/>
    <dbReference type="eggNOG" id="ENOG502QPST">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021855_2_1_1"/>
    <dbReference type="InParanoid" id="G3AGW1"/>
    <dbReference type="OMA" id="QDHTECM"/>
    <dbReference type="OrthoDB" id="421038at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903561">
        <property type="term" value="C:extracellular vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030445">
        <property type="term" value="C:yeast-form cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000005">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000038">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF2">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 10" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9 10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005131599" description="1,3-beta-glucanosyltransferase" evidence="10">
        <location>
            <begin position="24"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="529"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="13">
        <location>
            <begin position="378"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="337"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="484"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="340"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="491"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="549" mass="58946" checksum="64302A07AECCBBF1" modified="2011-11-16" version="1">MLFKSLLPSAIVAISTFISSVSADDLPPIEIVGNKFFYSNNGSQFFMKGIAYQQNTADSNSTFVDPLADADACKRDIPYMQAVDTNVIRVYALDTTQDHTECMQLLQEAGIYVVADLSQPDESINRASPSWDLDLLKRYTSVVDLFHNYTNILGFFAGNEVTNEVSNTDASAFVKAAIRDTKAYIKAKGYRTIPVGYSANDDTDIRQSLAQYFACGDDDERADFFGINMYEWCGSSSFTTSGYSTITKDYANLNIPLFFSEYGCNKVTPRKFQEVATIYGDQMTDVWSGAIVYMYFQEENDYGLVSIDGSGKVSTLQDYNNYKSQILQVSPSGVKAADESSSSLSPTSCPTNTDNWEASTELPPTPDKDVCNCMSESLSCVVSPKVKSSKYGDLFDYVCDQIDCSGIGANGTTGEYGAYSPCEAADKLSFVLNLYYEAQGGDDSACDFDGSATLQKAQTASSCSAILKSAGTSGLGTVVGAIHTDESDSQSNGSSTNGSNSSKTTSGSSSSGTSAAKKNSANSNSAMTLWTKIGAVGVSMVVGFGMIMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGY9</accession>
    <name>G3AGY9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Survival factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_53101</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SVF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372074.1">
        <property type="nucleotide sequence ID" value="XM_007372012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGY9"/>
    <dbReference type="FunCoup" id="G3AGY9">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGY9"/>
    <dbReference type="GeneID" id="18871760"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53101"/>
    <dbReference type="eggNOG" id="ENOG502QQY3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030205_2_0_1"/>
    <dbReference type="InParanoid" id="G3AGY9"/>
    <dbReference type="OMA" id="AFWPRCV"/>
    <dbReference type="OrthoDB" id="2590239at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051385">
        <property type="entry name" value="Ceramide-binding_SVF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033394">
        <property type="entry name" value="Svf1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013931">
        <property type="entry name" value="Svf1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47107:SF1">
        <property type="entry name" value="CERAMIDE-BINDING PROTEIN SVF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47107">
        <property type="entry name" value="SVF1-LIKE PROTEIN YDR222W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08622">
        <property type="entry name" value="Svf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17187">
        <property type="entry name" value="Svf1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159245">
        <property type="entry name" value="AttH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Svf1-like N-terminal" evidence="4">
        <location>
            <begin position="56"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Svf1-like C-terminal" evidence="5">
        <location>
            <begin position="226"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="387" mass="43113" checksum="EBEFCC2AA85C4618" modified="2011-11-16" version="1">MLKWVQSGLSVVAGTAEPEYGADAIQPITKRIVNNQVSRPTVAEDFNWLEPGSTNVETKTFYFTDLKSGSTGFAQVIHSNVMGVHTTAQFTFRLFNSKTPELGIWTSTKLENFRLEGANFYADGISLELIDNKTFKLNFSANPESLVELEMTRLTDGVIFGEDGFTFYGEDVENPWGSMRHAFWPRCSVAGKITTTKTATEANAEPVTKEIPIEGYTMFVFALQGMKPHHAAKSWNFLNFQSENYSAVQMEFTTPRSYAETRVNVGMICNNEKVLYATVNNKVVHLDPHDDVEIGWPVPKSIEFHYLKDAANPDDEGVEVGVVKGDLKTLVERVDVMAEIPQLVKTIVSNVAGAKPYIYQYCDELELTVDGITESGVAYSEVTFISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHE7</accession>
    <name>G3AHE7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Kinetochore protein Sos7 coiled-coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_59533</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373695.1">
        <property type="nucleotide sequence ID" value="XM_007373633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHE7"/>
    <dbReference type="STRING" id="619300.G3AHE7"/>
    <dbReference type="GeneID" id="18872961"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59533"/>
    <dbReference type="eggNOG" id="ENOG502SYMX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1492596_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHE7"/>
    <dbReference type="OMA" id="HICENNQ"/>
    <dbReference type="OrthoDB" id="18959at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037475">
        <property type="entry name" value="Sos7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048781">
        <property type="entry name" value="Sos7_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37329">
        <property type="entry name" value="KINETOCHORE PROTEIN SOS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37329:SF1">
        <property type="entry name" value="KINETOCHORE PROTEIN SOS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20882">
        <property type="entry name" value="Sos7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Sos7 coiled-coil" evidence="2">
        <location>
            <begin position="51"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="84"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="181" mass="21570" checksum="BE79B3341A900131" modified="2011-11-16" version="1" fragment="single">METSLSKSYRSQLEYTPEYWILSAEKDFKSANIKLTNPRYIDDEIANEKNILSKLKFLYLEQETRYEFLKHICENNQDITDLQVEEIRESTQESKLRLKHLKQQLNQQIGDLETITKEVSDLYEKYLELTTTTLFDEAEELEEEIAKLKESEDNWETLDFMLQGSNNTKALEEQQKKLQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHM8</accession>
    <name>G3AHM8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Decapping nuclease</fullName>
            <ecNumber evidence="7">3.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_148716</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside-ribonucleotide in mRNA + H2O = a (N(7)-methyl 5'-triphosphoguanosine)-nucleoside + a 5'-end phospho-ribonucleoside in mRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66928"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17313"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:172876"/>
            <dbReference type="ChEBI" id="CHEBI:172877"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:66929"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 5'-end NAD(+)-phospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-ribonucleoside in mRNA + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60880"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:15698"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:144029"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:60881"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-ribonucleoside in mRNA + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78683"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:167618"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:78684"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DXO/Dom3Z family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="7"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373776.1">
        <property type="nucleotide sequence ID" value="XM_007373714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHM8"/>
    <dbReference type="STRING" id="619300.G3AHM8"/>
    <dbReference type="GeneID" id="18870798"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_148716"/>
    <dbReference type="eggNOG" id="KOG1982">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_674429_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHM8"/>
    <dbReference type="OMA" id="FNVIYWK"/>
    <dbReference type="OrthoDB" id="5853397at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034353">
        <property type="term" value="F:mRNA 5'-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110155">
        <property type="term" value="P:NAD-cap decapping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013961">
        <property type="entry name" value="RAI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039039">
        <property type="entry name" value="RAI1-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12395:SF9">
        <property type="entry name" value="DECAPPING AND EXORIBONUCLEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12395">
        <property type="entry name" value="DOM-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08652">
        <property type="entry name" value="RAI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RAI1-like" evidence="8">
        <location>
            <begin position="48"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="413" mass="47986" checksum="125069B727112C86" modified="2011-11-16" version="1">MIDKIKLSIFEKLNTKYLNSLEEPSYDDHEDAFIYNTLLKDASTDITPEEWLHFSKADTEDEPAFDDISGLKKLAIHIPDGTKKKHTRKKLKDPECESHSIIGSCMTLGFDNYQHPKLADIYSLKDLFKCVEHLVQEKNHSLQDFKILTLRRHLHLLLKVPIRKQSIQFHVIYWKGLIIFAYDWEPEAQYKKQNPPNGIERLLQYSGMNFENIVCGDDSADYKSRYYSVVNHTVNGVPLLYCAEIDCAIKSEPGLCNYVELKTHSKSADDKIKTVNKLNKKLLEAYCQNKLVGCQHLVMGYRTLDFKLASIKTFKIGEVANIVNNDPIFLKHGCAINTKGIFRWYQLVIQWLVFQEKNLKNQDEATVYRLSFDREDELMDSYLSLTKVDKEESAMIFNSTVPEWFQTFVISQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI31</accession>
    <name>G3AI31_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">aspartate--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_59754</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tRNA(Asp) + L-aspartate + ATP = L-aspartyl-tRNA(Asp) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9660"/>
            <dbReference type="Rhea" id="RHEA-COMP:9678"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.12" evidence="3"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373929.1">
        <property type="nucleotide sequence ID" value="XM_007373867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI31"/>
    <dbReference type="FunCoup" id="G3AI31">
        <property type="interactions" value="1255"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI31"/>
    <dbReference type="GeneID" id="18873067"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59754"/>
    <dbReference type="eggNOG" id="KOG0556">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004553_2_1_1"/>
    <dbReference type="InParanoid" id="G3AI31"/>
    <dbReference type="OMA" id="WVHEIRD"/>
    <dbReference type="OrthoDB" id="372395at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04320">
        <property type="entry name" value="AspRS_cyto_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000013">
        <property type="entry name" value="Aspartate--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02075">
        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00458">
        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003483">
        <property type="entry name" value="PRK05159.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450:SF1">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450">
        <property type="entry name" value="ASPARTYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="11">
        <location>
            <begin position="127"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW34345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="436" mass="49871" checksum="FEA0B808E27E9EAF" modified="2011-11-16" version="1">MVFLTLRQQTDLIQALVRTNKETDDKAISKRMVKWAGSINLESIVLIEGKVSKVDELIKSATVQDVEIQIEKIYTIQETPEQLPLLLEDASRSDAEAEAKGLPIVNLDTRLDARVIDLRTPTNQAIFKIQSGVCQLFREFLSKKQFTEIHTPKIIAAASEGGSNVFEINYFKGSAFLAQSPQLYKQQLIAGDFERVFEIAPVFRAENSNTHRHMTEFTGLDLEMAFEEHYDEVLAVLEELFVFIFTELKTRFGKEIATVRNQFPVEEFKIPADGKMVKLHFKEGIQMLRDAGKEVDDFEDLSTENEKLLGKLVRDKYDTDFYILDKFPLAVRPFYTMPDAEDPRYSNSYDFFMRGEEILSGAQRIHDPELLKTRIQAHDVDPNVPGLREYVDAFTYGCPPHAGGGIGLERVVMFFLDLKNIRRASLFPRDPKRLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI91</accession>
    <name>G3AI91_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="8">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_147526</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="8"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373242.1">
        <property type="nucleotide sequence ID" value="XM_007373180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI91"/>
    <dbReference type="FunCoup" id="G3AI91">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI91"/>
    <dbReference type="GeneID" id="18870755"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_147526"/>
    <dbReference type="eggNOG" id="KOG2108">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004585_7_0_1"/>
    <dbReference type="InParanoid" id="G3AI91"/>
    <dbReference type="OMA" id="ENGWRGL"/>
    <dbReference type="OrthoDB" id="1470711at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17932">
        <property type="entry name" value="DEXQc_UvrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070:SF46">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE HMI1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="11">
        <location>
            <begin position="14"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="12">
        <location>
            <begin position="309"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW33658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="723" mass="82440" checksum="19C4260D502C580D" modified="2011-11-16" version="1">MTITSVESDDVNLTETQLEAVRFDHDPKTLLSIQAGPGSGKTLTLIHRIKYLIDEKGVLPEGIIVLSMTNRTVNSLRANLSRLIGQTRAEKVKIRTFHSFAANLVDSYIAEYFPGKAIKVLLDDVSWRSFAAFFLNPSHMRGEKVKKYKDKVLNIAELERWINQLKCGESTLEHVAKQLKVPETELKESIQYLEDNGMIRYSDFISDAIELMEKSLSIENPIGELANVEVVIVDEFQDMQPNLLQLIQNITRYPSNSGENKHLTIAGDINQCIYEFLKSSPNITNEFIEKFNSWKIDQIVLKETFRLSPEVLEAAVDVALKPAGLGESMQLFESTKESSFKPVLYSLSNELEEYDFIAEEIWRLICQSGGLLQFSDFIILVRSNREVDLISKKLSQQHGFDVNKFTSVPDWIQGNTHIFLDVLNMLKNGFGSDFALLCIMMKLGANKPFLKKLLLSYNKWKLELPDRKRNLLEEYVYTLPSEKDPIFNTDVKKHLKLKILKFLTVVRQERDSSPLIRPKQVIQSIVNIVHGTELMDYINKVPATEQQRGEIVLKNLNSFSKSVKFAYGRYIMAKPDCTFIEFFLHTFSEDEPVIDKKSINISTVHKAKGLEFPVVFIPGCKSHYAKFPSSPWRNMNGVHAPSAEEARLLFVAMTRAKHLLYIGSINSSKWSKLFHKEPEKINTEYVSAMASSLNRKMPSADAIITGNQVYKSLGIASRFIKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIB1</accession>
    <name>G3AIB1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_59049</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373264.1">
        <property type="nucleotide sequence ID" value="XM_007373202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIB1"/>
    <dbReference type="FunCoup" id="G3AIB1">
        <property type="interactions" value="1093"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIB1"/>
    <dbReference type="GeneID" id="18872742"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59049"/>
    <dbReference type="eggNOG" id="KOG3428">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123956_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIB1"/>
    <dbReference type="OMA" id="VKMTLRH"/>
    <dbReference type="OrthoDB" id="9626941at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036261">
        <property type="term" value="P:7-methylguanosine cap hypermethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="5">
        <location>
            <begin position="5"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="90"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW33680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="123" mass="13467" checksum="B6535052E1B6B4DA" modified="2011-11-16" version="1">MKLVRFLMNIPNSTNQPLTVELKNGTTINGTLMSCSPSMNLSLKNVKLQQPLQDATLLQFINIRGNQIRQILLPDELNIDSILAKSVTKIKKQGAGPSAKPATTNKRAGGPMRGGKTRRPRAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIQ5</accession>
    <name>G3AIQ5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_133963</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374055.1">
        <property type="nucleotide sequence ID" value="XM_007373993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIQ5"/>
    <dbReference type="GeneID" id="18869774"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133963"/>
    <dbReference type="eggNOG" id="KOG1150">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070940_1_0_1"/>
    <dbReference type="InParanoid" id="G3AIQ5"/>
    <dbReference type="OMA" id="NWEDERD"/>
    <dbReference type="OrthoDB" id="342454at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46620">
        <property type="entry name" value="J DOMAIN-CONTAINING PROTEIN SPF31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46620:SF1">
        <property type="entry name" value="J DOMAIN-CONTAINING PROTEIN SPF31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="33"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="144"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="206" mass="24546" checksum="F7BC0EF52AA76190" modified="2011-11-16" version="1">MNNDELERILSLEESALNRTAEIERVLNCNEFDYFDILQLNPLSTADDLGSIIKRVYRKKSLLIHPDKTDHPRASDAFDRLKKAEKVLSCANEQEEEFKEKNRLIAIYHSVTSEGKDYDLDKVKKQVAEILQDEINEQELQVLYQQTAKQRKHEQEQKLRQERELKKQLESKWEDERDVRVKNWRDYSFKIDKKKKKKTGKPKVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIX3</accession>
    <name>G3AIX3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Vacuolar ATPase assembly protein VMA22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_54071</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373368.1">
        <property type="nucleotide sequence ID" value="XM_007373306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIX3"/>
    <dbReference type="STRING" id="619300.G3AIX3"/>
    <dbReference type="GeneID" id="18871830"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54071"/>
    <dbReference type="eggNOG" id="ENOG502SBHH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089394_1_0_1"/>
    <dbReference type="InParanoid" id="G3AIX3"/>
    <dbReference type="OMA" id="RANYHNK"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990871">
        <property type="term" value="C:Vma12-Vma22 assembly complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040357">
        <property type="entry name" value="Vma22/CCDC115"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31996">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31996:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21730">
        <property type="entry name" value="Vma22_CCDC115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="84"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00093634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="172" mass="20246" checksum="501DD223A0B2CC8C" modified="2011-11-16" version="1">MTEIQSQLDKKTIELLILIDKYENVVNNNYRTNFINGFVNLSKANYEHTGRGFERRYGVDSLDLRPRLACLEVNEEFHLVDLQQKTKKEEKREQMESSLKNRKTKQTKSKSSGVEEFEEDKVKDPMLQFGGIVPYQLRQSQAFFKKAIENSLEVVKLQQEIDTLIQEIEKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ64</accession>
    <name>G3AJ64_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">U6 snRNA-associated Sm-like protein LSm1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LSM1</name>
        <name evidence="9" type="ORF">SPAPADRAFT_54105</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the cytoplasmic LSM1-LSM7 complex which is involved in mRNA degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the heptameric LSM1-LSM7 complex that forms a seven-membered ring structure with a donut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373405.1">
        <property type="nucleotide sequence ID" value="XM_007373343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ64"/>
    <dbReference type="FunCoup" id="G3AJ64">
        <property type="interactions" value="444"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ64"/>
    <dbReference type="GeneID" id="18871837"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54105"/>
    <dbReference type="eggNOG" id="KOG1782">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_0_1_1"/>
    <dbReference type="InParanoid" id="G3AJ64"/>
    <dbReference type="OMA" id="FMVRGEN"/>
    <dbReference type="OrthoDB" id="10263346at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01728">
        <property type="entry name" value="LSm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000045">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034104">
        <property type="entry name" value="Lsm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044642">
        <property type="entry name" value="PTHR15588"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588">
        <property type="entry name" value="LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588:SF8">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Sm" evidence="8">
        <location>
            <begin position="37"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="166" mass="19119" checksum="9D2A840263C722D7" modified="2011-11-16" version="1">MSATPEPSPAPVQTQAPARDPLAPNVEDLYLESYAFTTAAALVGSVDRKIFVLLRDGRNLFGILRTFDQFANLVLQDTYERIYLDDKFAEVYRGVFMVRGENVVMMGELDIDREDDHLEVMQQIDFREAEDELKQKHKQIVSEQKIRSKKLLEKGLINDFVRGDMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ75</accession>
    <name>G3AJ75_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_65056</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373414.1">
        <property type="nucleotide sequence ID" value="XM_007373352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ75"/>
    <dbReference type="GeneID" id="18875014"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65056"/>
    <dbReference type="HOGENOM" id="CLU_1262212_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ75"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="219" mass="26619" checksum="9C0548EE9E27AC08" modified="2011-11-16" version="1">MLRIYCKNKERLNLKHKRIIYYDNVNKNIKVKRKEKDFITLENYVYLDKNEKTMYSTRYPTKSIKIENENDIFLALYYPTDVIYRNLAVNYYNEEDRIYAEEHNECVNQRFLALKTTEEIIDPCDSMYLTYIKDKDDRYKLDKVFYVVSNKTIYLGTNVSLTKDNFIKVDKIKDRERNLLELFLIKFKCDIKKVEQQSVYSLYVNDFDILEEVISQQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ90</accession>
    <name>G3AJ90_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTH APSES-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_59209</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EFG1/PHD1/stuA family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373431.1">
        <property type="nucleotide sequence ID" value="XM_007373369.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ90"/>
    <dbReference type="STRING" id="619300.G3AJ90"/>
    <dbReference type="GeneID" id="18872821"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59209"/>
    <dbReference type="eggNOG" id="ENOG502QW2C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032570_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ90"/>
    <dbReference type="OrthoDB" id="5407653at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.260.10:FF:000003">
        <property type="entry name" value="Ascospore maturation 1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.10">
        <property type="entry name" value="Transcription regulator HTH, APSES-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029790">
        <property type="entry name" value="EFG1/Phd1/StuA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018004">
        <property type="entry name" value="KilA/APSES_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47792">
        <property type="entry name" value="PROTEIN SOK2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47792:SF1">
        <property type="entry name" value="PROTEIN SOK2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04383">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01252">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH APSES-type" evidence="6">
        <location>
            <begin position="72"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="183"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="319"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="183"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="208"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="237"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="249"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="319"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="351"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="381" mass="41669" checksum="6EF22D991A6A9E3A" modified="2011-11-16" version="1">MAGAPGTGAATGNNYYQQSNQAYGNRGYPQTGAGAQTAAAAAAAASSNPDLLAGSGANSTVGQFQPPGIRPRVTTTMWEDEKTLCYQVDANNVSVVRRADNNMINGTKLLNVAQMTRGRRDGILKSEKVRHVVKIGSMHLKGVWIPFERALSMAQREGIVDLLYPLFVRDIKRVIQTGVTPAAASAATGGAQQTSPPVNNKMGAGAVQQNYYSQYNQQQQQQQPQQPQQQYGQQYPTGTPNQGQASIVTPGPTNQQSPQQQNPAVNDPQYQQVYGGYQQQYYNGAIATPNNPNNQYYQGYYNGSTYGQQQAYPYGYQQVQQGIPQQQQQQQQPMRQQQPLQQHPHQVGDSQTNAQQHTQPPQQVQKSPGKSPPTSDKKEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJE7</accession>
    <name>G3AJE7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_60040</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>an acyl-CoA + acetyl-CoA = a 3-oxoacyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:21564"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="2.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374190.1">
        <property type="nucleotide sequence ID" value="XM_007374128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJE7"/>
    <dbReference type="STRING" id="619300.G3AJE7"/>
    <dbReference type="GeneID" id="18873191"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60040"/>
    <dbReference type="eggNOG" id="KOG1389">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_1_1_1"/>
    <dbReference type="InParanoid" id="G3AJE7"/>
    <dbReference type="OMA" id="MTAFPEP"/>
    <dbReference type="OrthoDB" id="5404651at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003988">
        <property type="term" value="F:acetyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050215">
        <property type="entry name" value="Thiolase-like_sf_Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853">
        <property type="entry name" value="3-KETOACYL-COA THIOLASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853:SF8">
        <property type="entry name" value="3-KETOACYL-COA THIOLASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="11">
        <location>
            <begin position="27"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="12">
        <location>
            <begin position="287"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="354" mass="37810" checksum="B46A28A7BE2EF31F" modified="2011-11-16" version="1" fragment="single">MDRLNQLSGQLSPSSKQALLQKNPDDVVIVAAYRTAITKGFKGSFKDVGSEYILTHFLKEFLDKTGVDPSLIEDVAMGNVLNQAAGATEHRGACLAAGIPYTAGLIAINRFCSSGLMAISEIANKIRVGEIDCGIAGGVESMSKNYSAANLTPKVDPSLLKNPEMAKCLIPMGYTNENVANKYNISRERQDVFSADSYQKAEKAVKNGAFKDEILPIKALLRNDGQEKEIVVDTDEGPRPNVTAESLSKIKPAFKGTTHAGNASQISDGAAAVLLMRRSVAEAKGYEIQAKYIACSVAGVPPEIMGVGPAYAIPEVLKRTGLTVSDVDVFEINEAFAAQCLYSAEACNVPKEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJK8</accession>
    <name>G3AJK8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial ATPase complex subunit ATP10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_59285</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373495.1">
        <property type="nucleotide sequence ID" value="XM_007373433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJK8"/>
    <dbReference type="FunCoup" id="G3AJK8">
        <property type="interactions" value="152"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJK8"/>
    <dbReference type="GeneID" id="18872852"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59285"/>
    <dbReference type="eggNOG" id="KOG4614">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047290_2_0_1"/>
    <dbReference type="InParanoid" id="G3AJK8"/>
    <dbReference type="OMA" id="YFPNFHG"/>
    <dbReference type="OrthoDB" id="17089at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007849">
        <property type="entry name" value="ATP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28106">
        <property type="entry name" value="MITOCHONDRIAL ATPASE COMPLEX SUBUNIT ATP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28106:SF1">
        <property type="entry name" value="MITOCHONDRIAL ATPASE COMPLEX SUBUNIT ATP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05176">
        <property type="entry name" value="ATP-synt_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="279" mass="31978" checksum="CED23DE5B2180B9E" modified="2011-11-16" version="1">MNRQAIRTIKTKPFSAVTSTVASATQAAQTKSIPITKPFGLDKPVLLNHTIPENQYNLSSIWHNITSVEGRARRQKQLDYDIKHSALFDAKSFNNVQGKIFTPPPAYFKSQYSKYFPDFIAQTLGGKNLSLYDVLEKKVSIVNLFSTVTGQQCVNSYYKVEGKDLYAKDYSSFQETYPNCQIIDINVPQSWLKGFLLNLSINNLKKMIPQSRYDRYFILPDQIFQYEVRDSLLCSNSCSGYIYVLDSKGRVRWATSGYSTEEDLKLMWKVVKGLNNEKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJQ3</accession>
    <name>G3AJQ3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LSM complex subunit LSM3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LSM3</name>
        <name evidence="11" type="ORF">SPAPADRAFT_59353</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">LSm subunits form a heteromer with a doughnut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373538.1">
        <property type="nucleotide sequence ID" value="XM_007373476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJQ3"/>
    <dbReference type="FunCoup" id="G3AJQ3">
        <property type="interactions" value="552"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJQ3"/>
    <dbReference type="GeneID" id="18872878"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59353"/>
    <dbReference type="eggNOG" id="KOG3460">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_5_1_1"/>
    <dbReference type="InParanoid" id="G3AJQ3"/>
    <dbReference type="OMA" id="FDSHCNI"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140445">
        <property type="term" value="C:chromosome, telomeric repeat region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008266">
        <property type="term" value="F:poly(U) RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01730">
        <property type="entry name" value="LSm3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034105">
        <property type="entry name" value="Lsm3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040002">
        <property type="entry name" value="Sm-like_LSM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13110">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="6"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="80" mass="9235" checksum="01F896C25E4A66AC" modified="2011-11-16" version="1">MSTKEEPLDLIRFHLDEFVLIKLRGAREIKGKLQGYDSHCNMVLSEAEEYIYNDSNDDVETKKTEMVFVRGDSVILISPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK16</accession>
    <name>G3AK16_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_59490</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373651.1">
        <property type="nucleotide sequence ID" value="XM_007373589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK16"/>
    <dbReference type="GeneID" id="18872940"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59490"/>
    <dbReference type="HOGENOM" id="CLU_2706395_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK16"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="73" mass="8243" checksum="2475158B6E6DF951" modified="2011-11-16" version="1">MKLYIISSHKSKNLSFVLVLSSNHSLERQECSSGQKLLAIFSYSGYLTFQVLSKLTTREQVILPDSLTHILAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKM0</accession>
    <name>G3AKM0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_60962</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375140.1">
        <property type="nucleotide sequence ID" value="XM_007375078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKM0"/>
    <dbReference type="FunCoup" id="G3AKM0">
        <property type="interactions" value="1403"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKM0"/>
    <dbReference type="GeneID" id="18873656"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60962"/>
    <dbReference type="eggNOG" id="KOG2624">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010974_5_0_1"/>
    <dbReference type="InParanoid" id="G3AKM0"/>
    <dbReference type="OMA" id="YACEEHT"/>
    <dbReference type="OrthoDB" id="9974421at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004771">
        <property type="term" value="F:sterol ester esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000095">
        <property type="entry name" value="Triglyceride lipase-cholesterol esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="8">
        <location>
            <begin position="147"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="531" mass="61455" checksum="93EE2D7D76D6A36F" modified="2011-11-16" version="1">MSIPILGRLHITDWPIIAISFLLAWIEFIISIITNSLPSHVIDVFTVVTKFIYRFTRNPINLIIRHKKDDIHQDEEKFTYSYINSDLPNLTRKQYQTMIELLNADRIEQMVQLFGYDIESRIVKTKDDYLLTIHRIKGRDRPSNGKVIYLHHGLLMNCEIWCTMIEKYNNLPFLLYDLGYDVWMGNNRGSKYSQRHLFYKSNSVRFWDFSLDEFALFDIPNTIDYILKVSGAEKLTYIGFSQGSAQAFASVSVNIDLNDKIDQLIAISPATTPHGLYSKFLDILLKSSPNITYLLFSRKVLMPSCLFWQKIMYPPLFDTMIDISNYLLFNWKAENIDKLQKLSSYAHLYSTTSVKCAVHWFQVMSSKNFQMYHDATTSLSGLNPISYPLKNIQIPIHLIYGTADSLVDIEVMKSQLPDKTTTVHPVPNYEHLDNLWGRDVHELVFKEVLRRLGENTDDLFYRLSLLENDRNPKLIEDIIVDVGEDEATLNQQSYLKMANNSNSNGLSPVSHVENLDYGTDRRSTVGTGIFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALC5</accession>
    <name>G3ALC5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Branched-chain-amino-acid aminotransferase</fullName>
            <ecNumber evidence="16">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">BAT2</name>
        <name evidence="17" type="ORF">SPAPADRAFT_60476</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 2-oxocarboxylate + L-methionine = 4-methylsulfanyl-2-oxobutanoate + an L-alpha-amino acid</text>
            <dbReference type="Rhea" id="RHEA:31763"/>
            <dbReference type="ChEBI" id="CHEBI:16723"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:31765"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 6/6.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 14">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="16"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374682.1">
        <property type="nucleotide sequence ID" value="XM_007374620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALC5"/>
    <dbReference type="FunCoup" id="G3ALC5">
        <property type="interactions" value="682"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALC5"/>
    <dbReference type="GeneID" id="18873433"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60476"/>
    <dbReference type="eggNOG" id="KOG0975">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031922_0_1_1"/>
    <dbReference type="InParanoid" id="G3ALC5"/>
    <dbReference type="OMA" id="LTEVFAC"/>
    <dbReference type="OrthoDB" id="1732691at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052656">
        <property type="term" value="F:L-isoleucine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052654">
        <property type="term" value="F:L-leucine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052655">
        <property type="term" value="F:L-valine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010326">
        <property type="term" value="F:methionine-oxo-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009083">
        <property type="term" value="P:branched-chain amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000004">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.10:FF:000005">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01123">
        <property type="entry name" value="ilvE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009897">
        <property type="entry name" value="PRK13357.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11825:SF44">
        <property type="entry name" value="BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11825">
        <property type="entry name" value="SUBGROUP IIII AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006468">
        <property type="entry name" value="BCAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 16 17" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="10 16" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9 15" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16 17" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="13">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR006468-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW33167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="399" mass="44170" checksum="2D392D2F29A509A4" modified="2011-11-16" version="1">MLLRTLLTNLRARSLPLAGSGMSIRFASTLAPLDSTKLTIEHTTTPKPKLAKEDLVFGKYFTDHMLEIEWTASSGWSTPKITPYHNLSLDPSTIVFHYAFELFEGMKAYRDSNGDIRTFRGDKNMIRMNKSAERIALPTFDGEELLKCIDKFLLVDRDFVPEGNGYSLYLRPTMIGTTASLGVGVPDKALLYVIASPVGPYYSSGFKPVSLEATDYAVRAWPGGVGDKKLGANYAPCVKPQLEAASRGYAQNLWLFGEEGYITEVGTMNVFFVFKNQDGKRELVTAPLDGTILEGVTRDSILELAKDRLDSAEWTISERKYTIGEVEERANKGELVEAFGAGTAAIVSPIKSIGFRDGRNIEVPVALGNSGELTSQVAEWIRKIQYGEESYKNWSRVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALE3</accession>
    <name>G3ALE3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60510</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374700.1">
        <property type="nucleotide sequence ID" value="XM_007374638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALE3"/>
    <dbReference type="GeneID" id="18873442"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60510"/>
    <dbReference type="HOGENOM" id="CLU_1300357_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALE3"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="212" mass="24943" checksum="502CF892C19B9332" modified="2011-11-16" version="1">MSSLHFAKQAEKKKLWLTKLAEYAALTNESPVPDQVVVLKFKKKILSKYNPHNNDKWPDVECHLREIAIECFGEHDFPFGYYWSYLYFSEVIEGLTKEFITHNMFEAFKVRYNSIENVKNDKELCSNYDRAKGRGLSLLRFVHFNKEIAYENTLAFTKLEMRAIENDYNRRVELYHENERKRRGWDVDYTASGVVGILLIGCSVFGLYLVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALJ4</accession>
    <name>G3ALJ4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_60571</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374752.1">
        <property type="nucleotide sequence ID" value="XM_007374690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALJ4"/>
    <dbReference type="STRING" id="619300.G3ALJ4"/>
    <dbReference type="GeneID" id="18873473"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60571"/>
    <dbReference type="eggNOG" id="KOG1729">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_987501_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALJ4"/>
    <dbReference type="OMA" id="HIDSTME"/>
    <dbReference type="OrthoDB" id="10018316at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15760">
        <property type="entry name" value="FYVE_scVPS27p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23164">
        <property type="entry name" value="EARLY ENDOSOME ANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23164:SF30">
        <property type="entry name" value="EARLY ENDOSOME ANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="6">
        <location>
            <begin position="128"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="203"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="242"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="284" mass="31628" checksum="1D4BFAD777EF1E38" modified="2011-11-16" version="1">MTNNGVIHSPTQMRSYSNSPELIVGGGRNNNSSPSPKVPHHIDSTMEPSYITQLQMFGQGDTSSSRASIKSIDSNMSIESGNSSMSGHQHPQYLSSGNKGYDHLLKAPPTRRHWVKDDTVLKCSIPTCPKVFNFFERRHHCRKCGGIFCKEHTSHLLYINHLAQFTTGGRGTLSKVCDNCIEEYNEFIKKEFGVNVSTHPPSVSPMAAETYTETQPSQPPNQFIPLASANKYVPQPYTGPVSPEQQAQQQQQYQQSQLQSQQRQPSLSEQVVGSVPANWSWSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALK0</accession>
    <name>G3ALK0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Kinetochore protein Spc24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_50142</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the NDC80 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Chromosome</location>
            <location evidence="12">Centromere</location>
            <location evidence="12">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the SPC24 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374758.1">
        <property type="nucleotide sequence ID" value="XM_007374696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALK0"/>
    <dbReference type="FunCoup" id="G3ALK0">
        <property type="interactions" value="120"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALK0"/>
    <dbReference type="GeneID" id="18871409"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50142"/>
    <dbReference type="HOGENOM" id="CLU_091441_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALK0"/>
    <dbReference type="OMA" id="LKLMVYR"/>
    <dbReference type="OrthoDB" id="3344830at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11565">
        <property type="entry name" value="RWD_Spc24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013252">
        <property type="entry name" value="Ndc80_Spc24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038066">
        <property type="entry name" value="Spc24_Fungi_globular_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22142">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22142:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN SPC24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08286">
        <property type="entry name" value="Spc24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143026">
        <property type="entry name" value="Kinetochore globular domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 12" id="KW-0132">Cell division</keyword>
    <keyword evidence="10 12" id="KW-0137">Centromere</keyword>
    <keyword evidence="2 12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 12" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5 12" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="Pumilio" evidence="11">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="123"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW33243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="229" mass="26500" checksum="127196813D51B718" modified="2011-11-16" version="1">MSTDKSGNGILDDIINRLNAQNEQQLIDSILVSIDNLKRDREEDIATITNHNTQLKEEIEQLQRKISLLYEFNDSTFEQVQNIATVDGLNDRFGFTKNDEIPRVLKSIFNKLTDLNISISKELTDLEQIIISYASERNRLEKENDDLLIKMNQVYEENRNREVTAQDANVTKLALYRNLGIKLENSNGNKDEEPDTIVIQKGDHVNMLKIDPDYNDFFITNQIWSHLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALN3</accession>
    <name>G3ALN3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mitochondrial outer membrane transport complex Sam37/metaxin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60611</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374791.1">
        <property type="nucleotide sequence ID" value="XM_007374729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALN3"/>
    <dbReference type="STRING" id="619300.G3ALN3"/>
    <dbReference type="GeneID" id="18873488"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60611"/>
    <dbReference type="eggNOG" id="KOG3028">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078884_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALN3"/>
    <dbReference type="OMA" id="PMWYNTP"/>
    <dbReference type="OrthoDB" id="5835136at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001401">
        <property type="term" value="C:SAM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050931">
        <property type="entry name" value="Mito_Protein_Transport_Metaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019564">
        <property type="entry name" value="Sam37/metaxin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289:SF41">
        <property type="entry name" value="FAILED AXON CONNECTIONS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289">
        <property type="entry name" value="METAXIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10568">
        <property type="entry name" value="Tom37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Mitochondrial outer membrane transport complex Sam37/metaxin N-terminal" evidence="7">
        <location>
            <begin position="20"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="341" mass="38997" checksum="B5601D1A8FCDC1A1" modified="2011-11-16" version="1">MLQLHVWGVDGEISLISPECLASAWLLSLVFPKNDFEIVTSSNTNISDINKLPVLITDDKKLNGFSEIVDFIDGNKFANESGYWSSRASSPREKLIHSCLLNLVETRVDVINQYNLYSNTKNYEKYTRKLFQNYFPFPMMYNQPLKFHNNAVAQVKLLGLSKNKTSFFDFTTTFQEEVAETEYFNSELSDDEDEEEKEVALSSLHEKQLIAKSKTKAALLESKNSLRCLVLLNHYLQEFIKTFKLLQNENENAFGYLFGGSPSACELLLYAYLHSLSSEKLPDRFVSSYLDLKHEKLVSFSKEQINNLQKSLSNSSFRPPQGAEIPSLFNEVGYLLGSIKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALS7</accession>
    <name>G3ALS7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_65737</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374201.1">
        <property type="nucleotide sequence ID" value="XM_007374139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALS7"/>
    <dbReference type="GeneID" id="18875060"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65737"/>
    <dbReference type="eggNOG" id="ENOG502RB2N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_552275_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALS7"/>
    <dbReference type="OrthoDB" id="4056983at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="524" mass="61436" checksum="A1E01508EADC9397" modified="2011-11-16" version="1">MISATIPKNFITFLKKKWGYDCGRYINLITSDPLAKIFNTSTSFKERDRIMDYVVVSMKNFLQRDVNDIALVYFENKIIQENYYRRLCSEFGDKLMVMINADTDNKQASLLRLTRGARVLLVTKSVTCCIDLPQVNHILFINCLVPVIDFLQVTGRGRGSCILNILYQDSEAIHDSSDYRPCEERIRVSKCLTKQVAHFYGIKVSKCQNCCDSNDSILEPHIVDLVKNVHYATETQFKELQFGTEQAPARKKFRSCTAEEKLDHFALQFEPEEWLEELIDNGAKYLKYPLTHMDASPESEYICTKCYRKHGKECNRGGFKALLVLKWILDDITYDDIKDMKVNGWGDEIYKWAQNHNLKHLYRTYLDKVEQFRNCLQVPSAYLVEGYGTRLDHLRKFLRHPFFLNEYLLEDPIPTEDAGIYEKAQRYGEEYMHADTRKLIQMSQFHNNYCRQCGNSKHSGECRYSQAIELFYIIFCIPAFRDLRRSRQLGLTINYFPHWLDAISKNGHHICILNDILGRDRVWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM14</accession>
    <name>G3AM14_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH APSES-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_66337</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374882.1">
        <property type="nucleotide sequence ID" value="XM_007374820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM14"/>
    <dbReference type="GeneID" id="18875098"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66337"/>
    <dbReference type="eggNOG" id="ENOG502S1IW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_485847_0_0_1"/>
    <dbReference type="InParanoid" id="G3AM14"/>
    <dbReference type="OrthoDB" id="5562739at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030907">
        <property type="term" value="C:MBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033309">
        <property type="term" value="C:SBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.10">
        <property type="entry name" value="Transcription regulator HTH, APSES-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051642">
        <property type="entry name" value="SWI6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828">
        <property type="entry name" value="ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR XBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH APSES-type" evidence="2">
        <location>
            <begin position="236"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="561" mass="62470" checksum="1AB182424951D3AC" modified="2011-11-16" version="1">MIPSTDYYNYHHQRRPSLPLPLPSATIALGSPFNIHHPAAIPNQQTHLLSPLPPISNSSSAVAAASSFQLPRFKLAYHTQDPITPPTKPITYPITPPSPQRNSSVGVSSFVLAPINSHYLSPSQAAPRSSALVSVHSLFNHQKSTGSSPKSKHSYYQSVFKYSKKDKSGGAMISRFPTTNIVKKQKHKPHKVNKPKPKSKQSSSIPSNTINYPITKVDINDHLNSIYASIEIKKYSTAAIDPFRPYLTVYEYSVNDNWIIWDYETGFVHLTGIWKASLNASTHQKADIVKLLESTPKQYQPYIKRIRGGFLKIQGTWLPYKLCRILARRFCYFIRFELIPLFGKDFPDYCLKPGERGFGELKLDEITDEQVLDLPKQNPLPVNIVNHISTPIKTTEILDHQFLPRPRPLPLPMETPDSSPKQHFSPNSDASNSSSTIASIFSNTHRTSSSSPNMSYTDMLDIVNASKCLQSIAQAQPYRGMCDVKSDDSSSDEETPNYTIKPIATPNLDITNAGIASSLLVAAGVLPNGKRDSMVSSLRTDIRKSPTVFRRGSMKINDLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM57</accession>
    <name>G3AM57_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Luciferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_135684</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the NtaA/SnaA/DszA monooxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374277.1">
        <property type="nucleotide sequence ID" value="XM_007374215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM57"/>
    <dbReference type="GeneID" id="18869908"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135684"/>
    <dbReference type="eggNOG" id="ENOG502QSR6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022256_0_0_1"/>
    <dbReference type="InParanoid" id="G3AM57"/>
    <dbReference type="OMA" id="HWIDLAK"/>
    <dbReference type="OrthoDB" id="5561043at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051260">
        <property type="entry name" value="Diverse_substr_monoxygenases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016215">
        <property type="entry name" value="NTA_MOA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03860">
        <property type="entry name" value="FMN_nitrolo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011">
        <property type="entry name" value="ALKANESULFONATE MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011:SF16">
        <property type="entry name" value="C2H2 FINGER DOMAIN TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000337">
        <property type="entry name" value="NTA_MOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Luciferase-like" evidence="6">
        <location>
            <begin position="42"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="503" mass="56256" checksum="C4C612E961CD61F0" modified="2011-11-16" version="1">MTSATDGQKKQRKPLLINAFDMACPGLQTPGLWKHPDDKSRDYNTIEYWTNLAKLLEKGKFNALFIPDVLGGYDVYEGPGNIAAAAKSGAQWPVNEPSAVISAMAAVTKNLSFAVTGATISEAPYHWVRRLATLDHLTKGRIGWNIVSGYLDSAARNLLNGEPLPDHDARYDRAQEYLEVVYQLFLSSWADDAVVLDKKRGIFSDPERIREINYQGEFFKVPGPNITEPTPQRLPVIVQAGSSSKGLKYAAQNAEAVFIVGMTPEGLGYKIKQLKEEVKKAGRDPNSVKVVQLVTVIVAPTHEEAVAKFKEYKELSDIEGAQALFGGWTGIDLAQYGWNDELDKLEGEGNAIRSMIDTWTKPFPGDPENLKKTRAYIADRVRIGGSGVVFIGTPEEVADEIEEWVDVSGVDGFNFTYTITPGSFEDLVELLIPELRRRGLAWDDYPKEGLTFRENIYGLDREEPTYLPSDHPAYNLRWRAGESRQEFEVKLKKTLEERFKKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM84</accession>
    <name>G3AM84_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L33-A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_60744</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL33 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374897.1">
        <property type="nucleotide sequence ID" value="XM_007374835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM84"/>
    <dbReference type="FunCoup" id="G3AM84">
        <property type="interactions" value="858"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM84"/>
    <dbReference type="GeneID" id="18873544"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60744"/>
    <dbReference type="eggNOG" id="KOG0887">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100745_2_0_1"/>
    <dbReference type="InParanoid" id="G3AM84"/>
    <dbReference type="OMA" id="YRTNKHH"/>
    <dbReference type="OrthoDB" id="1166329at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.190:FF:000001">
        <property type="entry name" value="60S ribosomal protein L35a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.190">
        <property type="entry name" value="translation elongation factor selb, chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00573">
        <property type="entry name" value="Ribosomal_eL33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001780">
        <property type="entry name" value="Ribosomal_eL33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018266">
        <property type="entry name" value="Ribosomal_eL33_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038661">
        <property type="entry name" value="Ribosomal_eL33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10902">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01247">
        <property type="entry name" value="Ribosomal_L35Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01105">
        <property type="entry name" value="RIBOSOMAL_L35AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="12118" checksum="2AD8FDC9335D75D3" modified="2011-11-16" version="1">MAESHRLYVKGKHISYQRSKNVTNPNVSLIQIEGVANPQDAKFYLGKRIAYVYRASKEVRGSKIRVIWGKVTRTHGNNGLVRANFKKNLPAKTFGASVRIMLYPSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMA7</accession>
    <name>G3AMA7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA 3'-phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_71256</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374920.1">
        <property type="nucleotide sequence ID" value="XM_007374858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMA7"/>
    <dbReference type="FunCoup" id="G3AMA7">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMA7"/>
    <dbReference type="GeneID" id="18875371"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_71256"/>
    <dbReference type="eggNOG" id="KOG2134">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014938_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMA7"/>
    <dbReference type="OMA" id="KPEIGMW"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046404">
        <property type="term" value="F:ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046403">
        <property type="term" value="F:polynucleotide 3'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013954">
        <property type="entry name" value="PNK3P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006551">
        <property type="entry name" value="Polynucleotide_phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01664">
        <property type="entry name" value="DNA-3'-Pase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12083">
        <property type="entry name" value="BIFUNCTIONAL POLYNUCLEOTIDE PHOSPHATASE/KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12083:SF9">
        <property type="entry name" value="BIFUNCTIONAL POLYNUCLEOTIDE PHOSPHATASE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08645">
        <property type="entry name" value="PNK3P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="27797" checksum="B4837BB67DC0BC01" modified="2011-11-16" version="1">MPVDITALLTKPKSIAKVSKPKLPSSSASTSRLPQTETKLNQSITLFATRWKTIGTHLIKNVPSSIDFASKQVKVAAFDLDGTLIETKSGYKFSRGPTDWKWWQDKNIMAKLQELISEGYLLVVFTNQGGVIPEKTSKSYTNFTSKLNYINAVVESKTGKHLLVFASPKRPTKGYGRSTEEQHNHTRKPEIGMWEALVEYLNSFGFEVDVENSFFVGDAAGRKKDFSDSDKKFADGAGLQFKTPEDIFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMA9</accession>
    <name>G3AMA9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_55289</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374922.1">
        <property type="nucleotide sequence ID" value="XM_007374860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMA9"/>
    <dbReference type="FunCoup" id="G3AMA9">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMA9"/>
    <dbReference type="GeneID" id="18871925"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55289"/>
    <dbReference type="eggNOG" id="ENOG502RXRN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107213_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMA9"/>
    <dbReference type="OMA" id="KYWQNEP"/>
    <dbReference type="OrthoDB" id="409928at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.30:FF:000006">
        <property type="entry name" value="40S ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="17344" checksum="89A702242C8F93C4" modified="2011-11-16" version="1">MSLVHLANLCAHLKNCNNVRVATTSIPYTRMHLQTVLGLHKQGFISGIKRGSLNGPDVEPTEVTPDNISSRRLWIDLKYRNNRSVIRDIQLISKPGKRVNLSQEDVKALASGLPVRKITPLQPAEAIFIKADNEIYEVHEAAQRNLSGQALFRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMC2</accession>
    <name>G3AMC2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Trafficking protein particle complex subunit BET3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_151493</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Golgi apparatus</location>
            <location evidence="1 8">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374934.1">
        <property type="nucleotide sequence ID" value="XM_007374872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMC2"/>
    <dbReference type="FunCoup" id="G3AMC2">
        <property type="interactions" value="1073"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMC2"/>
    <dbReference type="GeneID" id="18870895"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_151493"/>
    <dbReference type="eggNOG" id="KOG3330">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087110_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMC2"/>
    <dbReference type="OMA" id="MVQMQVQ"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14942">
        <property type="entry name" value="TRAPPC3_bet3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1380.20:FF:000001">
        <property type="entry name" value="Trafficking protein particle complex subunit BET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016721">
        <property type="entry name" value="Bet3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13048">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018293">
        <property type="entry name" value="TRAPP_I_complex_Bet3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR018293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="191" mass="21772" checksum="6AD34423E141093A" modified="2011-11-16" version="1">MSRKAVGDDIWKNNVEKINSELFTLTYGSVVAQLCRDFNNNYHEVNNQLDKMGYNIGLRLIEEFLAKTGVRRCQTFKETSEVISKLGFKIFLNIQPTIENWSADGKSCSLILPEPNPLTEFVELPITADDKIAKELWYSQILCGVLRGALQMVQLDCDVQFVKDVLRGDDRTELRLKLNKILKDEVPAGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AME5</accession>
    <name>G3AME5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mannan polymerase II complex ANP1 subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60143</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ANP1/MMN9/VAN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374312.1">
        <property type="nucleotide sequence ID" value="XM_007374250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AME5"/>
    <dbReference type="STRING" id="619300.G3AME5"/>
    <dbReference type="GeneID" id="18873239"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60143"/>
    <dbReference type="eggNOG" id="ENOG502QVDT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017872_1_0_1"/>
    <dbReference type="InParanoid" id="G3AME5"/>
    <dbReference type="OrthoDB" id="204164at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052086">
        <property type="entry name" value="Mannan_Polymerase_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083">
        <property type="entry name" value="MANNAN POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083:SF2">
        <property type="entry name" value="MANNAN POLYMERASE II COMPLEX ANP1 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03452">
        <property type="entry name" value="Anp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="261" mass="30717" checksum="5AB572EA0AAC72C1" modified="2011-11-16" version="1" fragment="single">RKLMARARNWLWTVAIKPYHSFVYWRDADVETVPPTIVEDLMHHDKDVIVPNVWRPLPDWLGNQQPYDLNSWQESDGGLQLADTLDEDACIVEGYVEYQTWRPHLAYLRDPYGDPEVEMDLDGIGGVSILTKARVFRTGSHFPAFSFEKHAETEAFGKLSKRMGYSVVGLPHYVIWHIYEPSSDDLKHMEWMALEEVRQQEEAKIKKVYDKVWNKAFADVQDEWSKERQQYLKNTDMSKQRKIKVDWPGVDDWKIDQSQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMF7</accession>
    <name>G3AMF7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_60154</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374324.1">
        <property type="nucleotide sequence ID" value="XM_007374262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMF7"/>
    <dbReference type="FunCoup" id="G3AMF7">
        <property type="interactions" value="375"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMF7"/>
    <dbReference type="GeneID" id="18873246"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60154"/>
    <dbReference type="eggNOG" id="KOG4209">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_23_5_1"/>
    <dbReference type="InParanoid" id="G3AMF7"/>
    <dbReference type="OMA" id="EHASHAM"/>
    <dbReference type="OrthoDB" id="4726at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF12">
        <property type="entry name" value="EUKARYOTIC INITIATION FACTOR 4B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="33"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="105"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="116"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="171" mass="18878" checksum="447277C109CF698B" modified="2011-11-16" version="1">MSEEPAQPVQESNSQSDVDNAEKLAKQEEIDSRSIYVGNVDYQSTPEQLEQFFHVVGIIERVTILFDRFSGLPKGYAYIEFEKSVSVQQAIDELNGKEFRGRELRVTSKRTNLPGFRKRGGGRGRGRGGRGRGRGGRGRGRGGRGRGGRNSDETSENASVPTEPSEEVDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMX0</accession>
    <name>G3AMX0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Alternative oxidase</fullName>
            <ecNumber evidence="17">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">AOX2</name>
        <name evidence="19" type="ORF">SPAPADRAFT_61458</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes cyanide-resistant oxygen consumption. May increase respiration when the cytochrome respiratory pathway is restricted, or in response to low temperatures.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 17">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="16 17">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the alternative oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="17"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375660.1">
        <property type="nucleotide sequence ID" value="XM_007375598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMX0"/>
    <dbReference type="STRING" id="619300.G3AMX0"/>
    <dbReference type="GeneID" id="18873905"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61458"/>
    <dbReference type="eggNOG" id="ENOG502QSB5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041974_3_0_1"/>
    <dbReference type="InParanoid" id="G3AMX0"/>
    <dbReference type="OMA" id="RFLRWGM"/>
    <dbReference type="OrthoDB" id="16906at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098803">
        <property type="term" value="C:respiratory chain complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009916">
        <property type="term" value="F:alternative oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010230">
        <property type="term" value="P:alternative respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01053">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.140:FF:000002">
        <property type="entry name" value="Alternative oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.140">
        <property type="entry name" value="Alternative oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002680">
        <property type="entry name" value="AOX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038659">
        <property type="entry name" value="AOX_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31803">
        <property type="entry name" value="ALTERNATIVE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31803:SF3">
        <property type="entry name" value="ALTERNATIVE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01786">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005229">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12 16 17" id="KW-0408">Iron</keyword>
    <keyword evidence="14 17 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 16 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 17 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR005229-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW32384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="342" mass="39737" checksum="50A43100DAB7E361" modified="2011-11-16" version="1">MITTATLNKRLPIFTTSVCRYASTLAQQHPPPANDPKTSIFDISTKVYKETDIEKHNDTKFITHPIFPHPSFPQEECEKVMFEHREPKTLGDKISYHGMRFCRSAFDKVTGYKKLSGTDIREHDGTRYEMTEGKWLTRVIFLESIAGVPGFVASFIRHLHSLRLLKRDKAWIETLLDEAYNERMHLLTFIKLGKPSWFTRSIIYAGQGVFANIFFLCYLANPRFCHRFVGYLEEEAVSTYTHLVHELETPGKLTGFNDMKIPEIAVQYWPELTENSSFKDLILRIRADEAKHREVNHTLANLNQKSDRNPFAMQIEDYDKPQPNYGLKVTKGTGWEREDLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN30</accession>
    <name>G3AN30_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Succinate dehydrogenase assembly factor 2, mitochondrial</fullName>
            <shortName evidence="4">SDH assembly factor 2</shortName>
            <shortName evidence="4">SDHAF2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_61512</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Required for flavinylation (covalent attachment of FAD) of the flavoprotein subunit of the SDH catalytic dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with the flavoprotein subunit within the SDH catalytic dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SDHAF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375720.1">
        <property type="nucleotide sequence ID" value="XM_007375658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN30"/>
    <dbReference type="FunCoup" id="G3AN30">
        <property type="interactions" value="311"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN30"/>
    <dbReference type="GeneID" id="18873927"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61512"/>
    <dbReference type="eggNOG" id="KOG3326">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103054_0_1_1"/>
    <dbReference type="InParanoid" id="G3AN30"/>
    <dbReference type="OMA" id="YGKPQNP"/>
    <dbReference type="OrthoDB" id="284292at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.250:FF:000002">
        <property type="entry name" value="Succinate dehydrogenase assembly factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.250">
        <property type="entry name" value="Flavinator of succinate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03057">
        <property type="entry name" value="SDHAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005631">
        <property type="entry name" value="SDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036714">
        <property type="entry name" value="SDH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028882">
        <property type="entry name" value="SDHAF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12469">
        <property type="entry name" value="PROTEIN EMI5 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12469:SF2">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03937">
        <property type="entry name" value="Sdh5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109910">
        <property type="entry name" value="YgfY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="149" mass="17619" checksum="7C36E1DF65992E87" modified="2011-11-16" version="1">MIRINKRYLSNGRKCLANFGKPQNPPIDPTLHMKIQPIVRTGETIDIKRARLVYQSRKRGILESDLLLSRFASKYLNKFTMEELDEYDKLLDEADWDIYYWATKNFDVTPLPDKWKDSKILKLLQESAENKEKEILTMPELDLSDKQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN74</accession>
    <name>G3AN74_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Fatty acid synthase subunit beta</fullName>
            <ecNumber evidence="21">2.3.1.86</ecNumber>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="21">3-hydroxyacyl-[acyl-carrier-protein] dehydratase</fullName>
                <ecNumber evidence="21">4.2.1.59</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="21">Enoyl-[acyl-carrier-protein] reductase [NADH]</fullName>
                <ecNumber evidence="21">1.3.1.9</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="21">[Acyl-carrier-protein] acetyltransferase</fullName>
                <ecNumber evidence="21">2.3.1.38</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="21">[Acyl-carrier-protein] malonyltransferase</fullName>
                <ecNumber evidence="21">2.3.1.39</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="21">S-acyl fatty acid synthase thioesterase</fullName>
                <ecNumber evidence="21">3.1.2.14</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="24" type="ORF">SPAPADRAFT_138494</name>
    </gene>
    <organism evidence="25">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20 21">Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. The beta subunit contains domains for: [acyl-carrier-protein] acetyltransferase and malonyltransferase, S-acyl fatty acid synthase thioesterase, enoyl-[acyl-carrier-protein] reductase, and 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 21">
            <text>(9Z)-octadecenoyl-[ACP] + H2O = (9Z)-octadecenoate + holo-[ACP] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15057"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9924"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78783"/>
            <dbReference type="EC" id="3.1.2.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 21">
            <text>a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:13097"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="4.2.1.59"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 21">
            <text>a 2,3-saturated acyl-[ACP] + NAD(+) = a (2E)-enoyl-[ACP] + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10240"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9926"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78785"/>
            <dbReference type="EC" id="1.3.1.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 21">
            <text>acetyl-CoA + n malonyl-CoA + 2n NADPH + 4n H(+) = a long-chain-acyl-CoA + n CoA + n CO2 + 2n NADP(+).</text>
            <dbReference type="EC" id="2.3.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 21">
            <text>holo-[ACP] + acetyl-CoA = acetyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41788"/>
            <dbReference type="Rhea" id="RHEA-COMP:9621"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78446"/>
            <dbReference type="EC" id="2.3.1.38"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 21">
            <text>holo-[ACP] + malonyl-CoA = malonyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14 21">[Alpha(6)beta(6)] hexamers of two multifunctional subunits (alpha and beta).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 21">Belongs to the fungal fatty acid synthetase subunit beta family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.86" evidence="21"/>
    <dbReference type="EC" id="4.2.1.59" evidence="21"/>
    <dbReference type="EC" id="1.3.1.9" evidence="21"/>
    <dbReference type="EC" id="2.3.1.38" evidence="21"/>
    <dbReference type="EC" id="2.3.1.39" evidence="21"/>
    <dbReference type="EC" id="3.1.2.14" evidence="21"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375193.1">
        <property type="nucleotide sequence ID" value="XM_007375131.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AN74">
        <property type="interactions" value="670"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN74"/>
    <dbReference type="GeneID" id="18870123"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138494"/>
    <dbReference type="eggNOG" id="ENOG502QQJX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000114_5_0_1"/>
    <dbReference type="InParanoid" id="G3AN74"/>
    <dbReference type="OMA" id="HFMDNYG"/>
    <dbReference type="OrthoDB" id="5417908at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005835">
        <property type="term" value="C:fatty acid synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019171">
        <property type="term" value="F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004313">
        <property type="term" value="F:[acyl-carrier-protein] S-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004318">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141148">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016297">
        <property type="term" value="F:fatty acyl-[ACP] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004321">
        <property type="term" value="F:fatty-acyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016409">
        <property type="term" value="F:palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03447">
        <property type="entry name" value="FAS_MaoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.70:FF:000001">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000017">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000078">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.100:FF:000001">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.366.10:FF:000006">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000015">
        <property type="entry name" value="Fatty acid synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3330:FF:000001">
        <property type="entry name" value="Fatty acid synthase subunit beta dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.366.10:FF:000003">
        <property type="entry name" value="Fatty acid synthase subunit beta dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.60.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039569">
        <property type="entry name" value="FAS1-like_DH_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016452">
        <property type="entry name" value="Fas1/AflB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013565">
        <property type="entry name" value="Fas1/AflB-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041099">
        <property type="entry name" value="FAS1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040883">
        <property type="entry name" value="FAS_meander"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003965">
        <property type="entry name" value="Fatty_acid_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050830">
        <property type="entry name" value="Fungal_FAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032088">
        <property type="entry name" value="SAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982:SF21">
        <property type="entry name" value="FATTY ACID SYNTHASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982">
        <property type="entry name" value="MALONYL COA-ACYL CARRIER PROTEIN TRANSACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08354">
        <property type="entry name" value="Fas1-AflB-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13452">
        <property type="entry name" value="FAS1_DH_region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22235">
        <property type="entry name" value="FAS1_thioest_ins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17951">
        <property type="entry name" value="FAS_meander"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17828">
        <property type="entry name" value="FAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16073">
        <property type="entry name" value="SAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005562">
        <property type="entry name" value="FAS_yeast_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01483">
        <property type="entry name" value="FASYNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 21" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6 21" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 21" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 21" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 21" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 21" id="KW-0456">Lyase</keyword>
    <keyword evidence="13 21" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9 21" id="KW-0520">NAD</keyword>
    <keyword evidence="7 21" id="KW-0521">NADP</keyword>
    <keyword evidence="8 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 21" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Malonyl-CoA:ACP transacylase (MAT)" evidence="23">
        <location>
            <begin position="1684"/>
            <end position="2038"/>
        </location>
    </feature>
    <feature type="active site" description="For acetyltransferase activity" evidence="22">
        <location>
            <position position="280"/>
        </location>
    </feature>
    <feature type="active site" description="For malonyltransferase activity" evidence="22">
        <location>
            <position position="1828"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00058855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRNR" id="PIRNR005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR005562-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="EGW31917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="2071" mass="231625" checksum="03C33917EB8AFE6A" modified="2011-11-16" version="1">MTTSTSSHRPFQLVHGSIEHTLLVPNELFFNYSQLKEEFTKTLPEPTEGFADDNEPSSPAELYAKFLGFIASYADKEFVGQILKLSLQDFNSRFLDSNNENIHSFAATLLSDDVVYPTTPTKIKDNIIKNYYQAIIKSNTKIDKIESNLLYQAHKGDAKLAAIFGGQGNTDDYFEELRELYKVYGDLIYDDLIVPITTQLNKLVKQDSSSLDKIFTQGLNILSWLRHPETTPDQDYLLSVPVSCPVICIIQLCHYAITCKVLGLTPGEFRKYFIGSTGHSQGLVTAVAIASSDSWDSFLENSIKATSLLFYIGARCLITYPRTTLPPTMLQDSLDHGEGRPSPMLSVRDLTIEQVEAFIKQTNSHLPQEKHIAISLVNGARNLVVSGPPESLYGFNLNLRNKKAPNGLDQSRIPFSERKLKCSNRFLPIFAPFHSHLLADATETILEDVSQQNLNFKVSDLKIPVFDTFDGSNFQDFVENGETLVGRIVKCITELAVHWETSTKFQSTHILDFGPGGVSGLGVLTHRNKEGTGARIIVAGALDSNPIDDEYGFKQEIFHHSADNEIKWAPNWLNEYKPTLVKTAHNGKVFVKTKFSQLLGRAPLMVPGMTPTTVNPKTIIASLNAGYHIELAGGGYFAPQGMTAAIDEIVENIKPGYGLGINLIYVNPRMLQWGIPLIKELRDKGYPIQSLTIGAGVPSLEVATEYIETLGLTHLGLKPGSIDAISQVITIAKAHPNFPIVLQWTGGRGGGHHSFEDFHQPILQMYSKIRRCSNIVLVAGSGFGSDEDTYPYLTGSWSNEYNYPAMPFDGILFGSRVMTAKEAETSLEAKKLIATCKGVSDDKWELTYKKPTGGIITVRSEMGEPIHKIATRGVMLWKELDDTIFTQPKNKQLEILNKKKDYIIDKLNKDFQKPWFGKNAQGVCDLQDMTYQEVANRLIELMYVKKSQRWIDVSLRNFFGDFLRRVEERFTTASGAVSLIQNYNQLQTSPQQFTDDFFNKFEQAKQQLISEEDCDFFLMCCSRPTQKPAPFVPVLDDRFEFFFKKDSLWQSEDLETVVDEDVQRTCILHGPVAAQFTNKVNEPIGEILDSIHEGHIAKLIKDEYANDATKIPVVEYFGGSTAPVETVVENVKISTVGDKTIYEIASNVPEHDAWLRLLAGSELDWLKAFISTDRIVQGSKHIGNQVHDILKPVVHSRVEIVGAGSQKKLVGYENVKGDLKPVVEIQLTKGNVIQLNLIEHRTADGTPVALPFLYNYNPADGFAPILEVMTGRNDRIKEFYWKLWFGNDVPYDLNIDVEETIEGEQVTITGQDIAEFTHAIGNNCDAFVTRHGKVTLAPMDFAIVIGWRAIIKAIFPKTVDGDLLKLVHLSNGYKMIPGAAPLKKDDIVSTKAQIKRVVNQASGKQVEVVGTIYREGKPVMEVTSQFLYRGDYTDYENTFQKVTETPVQVSLKNSKDLAVLRSKEWFHLEQDVELLNQTLTFRCESTYKFKTADVYSSIKTTGQIYLELPTKEVVQVGQVDYEAGVSYGNPVTDYLQRNGKTIEEAVKFENVIPISSGEELTSKAPSTNEPYAIVSGDYNPIHVSRVFAAYAKLPGTITHGMYSSANIRALVEQWAANNVAARVRAFKCDFVGMVLPNDTLQTTLEHIGMINGRKIIKVETKNVETDSVILIGEAEIEQPITAYVFTGQGSQEQGMGMDLYNSSEVAREVWDKADVHFVNHYGFSILDIVQNNPNELTIHFGGAKGRKIRENYITMMFETIGEDGELKSEKIFKDIDETTTSYTFVSPTGLLSATQFTQPALTLMEKAAYEDIKSKGLIPSDIMFAGHSLGEYSALSSLANVMPIESLVDVVFYRGMTMQVAVPRDELGRSNYGMCAINPSRVSVTFNDAALRFVVDEVATVTGWLLEIVNYNVENQQYVAAGDLRSLDTLTNVLNVLKLNKIDIVKLQSMMSLEEVKQHLKEIIDECSKKSIAKPQPIDLERGFAVIPLKGISVPFHSSYLRSGVKPFQRFLCKKIPKSSVKPNDLIGKYIPNLTAKPFELTKEYFQDVYELTGSEKIKKILENWESYEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN99</accession>
    <name>G3AN99_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 6">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_61550</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FKBP-type PPIase family. FKBP2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 6"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375758.1">
        <property type="nucleotide sequence ID" value="XM_007375696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN99"/>
    <dbReference type="FunCoup" id="G3AN99">
        <property type="interactions" value="578"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN99"/>
    <dbReference type="GeneID" id="18873947"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61550"/>
    <dbReference type="eggNOG" id="KOG0549">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013615_8_1_1"/>
    <dbReference type="InParanoid" id="G3AN99"/>
    <dbReference type="OMA" id="PKTCDIQ"/>
    <dbReference type="OrthoDB" id="1902587at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000006">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044609">
        <property type="entry name" value="FKBP2/11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45779">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45779:SF7">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0697">Rotamase</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442493" description="peptidylprolyl isomerase" evidence="7">
        <location>
            <begin position="20"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="8">
        <location>
            <begin position="42"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="144" mass="15705" checksum="CDBCEF1F34A91788" modified="2011-11-16" version="1">MKASILSTVLLSALGVVSAASPDELKIKVITPVECHRKTRAGDYISVHYEGKLEDGTVFDSSYNRGVALPFNLGQGQVIKCWDEGLLDMCKGEVRELWCHPNVAYGERGIGPIPPNSALIFKAELFDIAGVDVSEETQPEKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANJ6</accession>
    <name>G3ANJ6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">High affinity glucose transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">HGT1</name>
        <name evidence="10" type="ORF">SPAPADRAFT_55953</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375801.1">
        <property type="nucleotide sequence ID" value="XM_007375739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANJ6"/>
    <dbReference type="STRING" id="619300.G3ANJ6"/>
    <dbReference type="GeneID" id="18871991"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55953"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_12_1"/>
    <dbReference type="InParanoid" id="G3ANJ6"/>
    <dbReference type="OMA" id="VFFMYPE"/>
    <dbReference type="OrthoDB" id="4142200at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000026">
        <property type="entry name" value="MFS quinate transporter QutD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF7">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="280"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="344"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="393"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="429"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="461"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="32"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="542" mass="60055" checksum="7A3FB667315D3BB9" modified="2011-11-16" version="1">MPSKLDVYCAKPALQVRTFLDKFPKIYNVYFIAMISTIAGMMFGFDISSMSAFIGTESYINFFDNPGSTIQGFITSCMALGSFFGSIASSFISEPFGRRLSLMVCSFLWMVGAAIQSSSQNRAQLIIGRIISGVGVGFGSSVAPIYGAELAPRKIRGLIGGLFQFSVTLGILIMFYLSYGLGHIHGVASFRISWGLQIVPGLLMFFGVFIIPESPRWLAKQDLWDQAEDIVARIQAKGDKEHPDVLVEIGEIKEQLLIEESAKSIGYATLFRKKYLRRTLTAIFAQIWQQLTGMNVMMYYIVYIFQMAGYSGNANLVASSIQYVLNTATTVPALFLFDRIGRRPLLVGGAIFMMIFQFGVAGILATYSVPWPDSGNESVTIRIPEDNKTASRGVIACCYLFVCSFASTWGVGIWVYCSEVWGDSRISQRGNALSTAANWIINFAIAMYTPSGFKNISWKTYIIYGVFCIVMAIHVFFGFPETRGKRLEEIGEMWDSNVPAWRSGSWEPHIPATSDAEIARKLSLEHKEGGLMEDSNSSADRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANK5</accession>
    <name>G3ANK5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SANT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_51080</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375810.1">
        <property type="nucleotide sequence ID" value="XM_007375748.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="G3ANK5">
        <property type="interactions" value="147"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANK5"/>
    <dbReference type="GeneID" id="18871512"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51080"/>
    <dbReference type="eggNOG" id="KOG1878">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_299559_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANK5"/>
    <dbReference type="OMA" id="LFCEGFC"/>
    <dbReference type="OrthoDB" id="10258692at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034967">
        <property type="term" value="C:Set3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000431">
        <property type="entry name" value="Set3C deacetylase complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051571">
        <property type="entry name" value="N-CoR_corepressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13992">
        <property type="entry name" value="NUCLEAR RECEPTOR CO-REPRESSOR RELATED NCOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13992:SF39">
        <property type="entry name" value="SMRTER, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="709"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="4">
        <location>
            <begin position="924"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="924"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="604"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="771"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1139"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="115"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="213"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="291"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="604"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="771"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="789"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="892"/>
            <end position="904"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1170" mass="131232" checksum="6DD019E480A9D8A4" modified="2011-11-16" version="1">MSSSNPRGHPPNSRYNDRNEYTDYGKSRRKNFYSLSNSNTTATSASTPVVNNQSAAPNSSLSRRDYKSNGPGSSSLTHSKDYNIPTTSSASASHKSFGRRSLSGRPNIPPNGANAAGTGESGAYSASSSSYYYPGYGNYSNDYYRGDSWRSERKSSGDSRVSTSNKPISAGGQASLSGSGPNSSYYQQYHRVSNGSEFRSSKDRYDSYVQDQSHSPTSSISSSSINTSKWKTNRSSLTNSLRYSSKDRIRGSGSGSGAVNAFGTVSGSNSVPIGSGKSTRASGGPTAADTYYPSRYSSVSSLYSNPKLVDRYSSLKRSYHDYSKPRDDKRRFSSKYESRKDIGPGNRASLSNVSTSYPLKEENITKEEEEQQSPDSVSQAEPEQHEEDMEDKDEEELDEDLDEEDNEENDEEDYSFEPTDLKLNEGKPDVDNESILEIEVSTKVEPTPKVEEIKVETKSEPVIIPPQPEDEDYYPDGCRYPLNKLETEYEALKKKYEQETLAYSKNGMSYLKYSLAKPISNLEDFPFFRDNFKVFIQVKGRLIDSLKDKNAKIHKKRLALWIEYSRGLELWEQERIKMDQQLRVLHPSDDEMRREIDSGDLRKQNQLQLQQQQQQQQQQFHEGTLDLPTVSSRRSRRHGDLVTTEAEFQEILLSLGQEEDEHPLVKAERVAAPIPDLILDPIERREIKFMDSNNIVHDKAQWAQRLHTSFLSNFSEREHELFCEGFCMYPKRFGAISRHMGGIRTSSECVDHYYITKKSVNYKQLLIQFKKRSSKKRRGKSVRSRNVSQTPASTPINEESSTLEQPTDETASEFENTTIPEVTPPVIVPPEIGRNEQYSEEELFTETGRRKRAAAPVFDKSKDKQQPAVDGPSKKKQRSVSRRDEPIPPNGSIDTENTELSPELTNPAIPTLSQGSDYSGADNRRKIISSYWSITEKNAFPKLLEEHGTKWTTIADHLTTKTATMVRNYYQRKAEKNGWNVIAEEANKRLAAKFAAVLNAGGDEAQKPSVQSIPVHSMYHANDIQGPGMIQPQQQPQISTGTFQHIIPSIANVVQQPIQHHHEVPKHVGPSISNLLADDSAPYQSPIQLQTPHQRSSIMNLLNNDSSPVKVEPQQEQMIPTPAPPAPPHRAMTNLRDLLNAPSSPAEPQAESNIKRGGIANLLSADPQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANT3</accession>
    <name>G3ANT3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Palmitoyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_61120</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DHHC palmitoyltransferase family. PFA5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="11"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375294.1">
        <property type="nucleotide sequence ID" value="XM_007375232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANT3"/>
    <dbReference type="FunCoup" id="G3ANT3">
        <property type="interactions" value="924"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANT3"/>
    <dbReference type="GeneID" id="18873724"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61120"/>
    <dbReference type="eggNOG" id="KOG1311">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_715709_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANT3"/>
    <dbReference type="OMA" id="DLFFCDE"/>
    <dbReference type="OrthoDB" id="331948at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF23">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="50"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="12">
        <location>
            <begin position="122"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW32018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="415" mass="48467" checksum="16D38BCC15C0CD30" modified="2011-11-16" version="1">MMDVSKFALCQTHIAKTAVPIAIILGFGYMNYACGYRLGYKEIYIHHSHAVAIVFWIIFGVLQLTVFLYWGLVVFIGPGKSPRFPPLDILGTGKYEDDDVLTPVPDLFFCDESGYPYYDAPTHSIKIDRTFYSKDVGYNVLKFDHYCLWIGTSLGQTNYEFFIKYMIWFMVLFFYDIIFTACYTRTSIARGLDHNFIAIFVLCAFWIIMIGALFIAHVRYINCDITTLDDITKQQRERYLRWAAHNTSEQEKPNKQQSKFVAWCLQSKTAPRFESGRRFVNAKSGDRRYVVEYSIYQHPFDLGVKKNWINLMFNGNRNHGLPESYYTNLRLFLSFYALAVPYLDIPLYFIARRKLIEGIGGEPDEEQGKREYLTRLYHMYSNYVSDDFRKFIDDKIAKGECSIPSYLKEAVDGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANX0</accession>
    <name>G3ANX0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Synaptobrevin/VAMP-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_61662</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375871.1">
        <property type="nucleotide sequence ID" value="XM_007375809.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANX0"/>
    <dbReference type="FunCoup" id="G3ANX0">
        <property type="interactions" value="316"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANX0"/>
    <dbReference type="GeneID" id="18873996"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61662"/>
    <dbReference type="eggNOG" id="KOG0860">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064620_2_1_1"/>
    <dbReference type="InParanoid" id="G3ANX0"/>
    <dbReference type="OMA" id="VRKKMWW"/>
    <dbReference type="OrthoDB" id="190375at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15874">
        <property type="entry name" value="R-SNARE_Snc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000004">
        <property type="entry name" value="Vesicle-associated membrane protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016444">
        <property type="entry name" value="Synaptobrevin/VAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45701">
        <property type="entry name" value="SYNAPTOBREVIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005409">
        <property type="entry name" value="Synaptobrevin_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="23"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="111" mass="12541" checksum="1988EEE1342EE021" modified="2011-11-16" version="1">MSSSVPYDPYIPNQTTGDQSNSRTAAIQAQIDDTVGIMRDNINKVAERGERLDSIENKTDNLAISAQGFRRGANRVRKDMWWKDFKMRMCLIFGIIIVIIVIVVPIAVHFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP47</accession>
    <name>G3AP47_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein uS17 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_61162</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375354.1">
        <property type="nucleotide sequence ID" value="XM_007375292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP47"/>
    <dbReference type="FunCoup" id="G3AP47">
        <property type="interactions" value="1166"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP47"/>
    <dbReference type="GeneID" id="18873751"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61162"/>
    <dbReference type="eggNOG" id="KOG1728">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073626_0_2_1"/>
    <dbReference type="InParanoid" id="G3AP47"/>
    <dbReference type="OMA" id="DYEKCPF"/>
    <dbReference type="OrthoDB" id="10254436at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.1000:FF:000001">
        <property type="entry name" value="40S ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028333">
        <property type="entry name" value="Ribosomal_uS17_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019979">
        <property type="entry name" value="Ribosomal_uS17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032440">
        <property type="entry name" value="Ribosomal_uS17_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006345">
        <property type="entry name" value="PRK08572.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03630">
        <property type="entry name" value="uS17_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744:SF9">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16205">
        <property type="entry name" value="Ribosomal_S17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00056">
        <property type="entry name" value="RIBOSOMAL_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS17 N-terminal" evidence="5">
        <location>
            <begin position="8"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW32078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="155" mass="17753" checksum="2593870F8B6071FC" modified="2011-11-16" version="1">MATELTVQSERAFQKQPHIFTNPKAKANRKTKRWYKEVGLGFKTPKTAIEGHYIDKKCPFVGTISIRGKILTGTVVSTKMHRTIIIRRDYLHYVPKYNRYEKRHKNVAAHLSPAFRVSEGDVVTVGQCRPLSKTVRFNVLKVAATSSKSKKFSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>G3APM9</accession>
    <name>G3APM9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_71679</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375476.1">
        <property type="nucleotide sequence ID" value="XM_007375414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APM9"/>
    <dbReference type="STRING" id="619300.G3APM9"/>
    <dbReference type="GeneID" id="18875393"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_71679"/>
    <dbReference type="eggNOG" id="ENOG502QVYJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007695_1_0_1"/>
    <dbReference type="InParanoid" id="G3APM9"/>
    <dbReference type="OMA" id="HYINTAF"/>
    <dbReference type="OrthoDB" id="3364175at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051127">
        <property type="entry name" value="Fungal_SecMet_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF6">
        <property type="entry name" value="PROLINE UTILIZATION TRANS-ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="25"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="63"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="117"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="158"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="233"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="251"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="279"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="293"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="880" mass="101259" checksum="368060B1849539B5" modified="2011-11-16" version="1">MISKAPVRKRIRRLPPEKRQKVSTACDSCKRRKFKCTGQTPCDVCVRKGTECTYTIIDKRSLKSERMAKKKLEEENQYRRQSIESDEQDDPFRKQSFSDTSSIQISPSNMSNVSPPHVSPPQVSPPPPPHMAQQIMQQQPQYLSQVSPSLPPPPPQQYQPQPYQQPYAPQYAPQYPPQQYAQYPQQYPPHHQQQQYPPQVPVGYQQPPPPPPMQPHLQQAHQSLPPQPLYDSRQPSQQISPTKNPHGILTPETTPNSNYNGNGHYIPKSLQPLLSFPLDNEEDTPPPEEDEDMTKKKSGDKKKDGISNQSGKSAILLVDKSNTFRYMGETSPLSLLYEARNIFVQYVGKTKLTEDLRGCPVIDKPAPIRNRVSIQLPPVEERDLYINNFKWNINDTFFIFDMDRFYKEIVEVVYRDPMNEHNKAVLIILYFVLAIGATYSDFSNGDPEAIKGAAYFDSGLNLLKDVVEDSEIWVVISHYLQFHYYQSILKKSTAWIHLNLAIKYAQSIGLHRSFVNEQFSKKSFETEYRKRLFRSLYSSDRISSVFIGRPLSINDYDWDDPTRFKSSNLPLTALDFNSKCQIELTKICTLIGRIVSNFYRDRIIDINRTRNLAIELKMWSKNLDPDLSIENILKPTEIPDNEAHGNTQILLMTHLLQLYAVMLLSRPFFMYEAVSAINAELKDTIKDKELVKDFRQAATKSSILAIKLMNHYINTAFKDCKRMECYIIITCCFYSSIMISITILSGGFEHEGYSESDLIDLLKSAKYVLNHFSKCNKGALRYAEISTDMIDALVHRHDKGGKAKHDTDSENESVDSEELDYRVLNDYNFIDDPNNNMQSLIEFQQFFVPQEIAPTGGSVDVNTTMPYDYGNYELFFGDKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3APP9</accession>
    <name>G3APP9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein mS38</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_61299</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the mitochondrion-specific ribosomal protein mS38 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375496.1">
        <property type="nucleotide sequence ID" value="XM_007375434.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APP9"/>
    <dbReference type="GeneID" id="18873829"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61299"/>
    <dbReference type="HOGENOM" id="CLU_2612682_0_0_1"/>
    <dbReference type="InParanoid" id="G3APP9"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013177">
        <property type="entry name" value="Ribosomal_mS38_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32035">
        <property type="entry name" value="AURORA KINASE A-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32035:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08213">
        <property type="entry name" value="COX24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01155">
        <property type="entry name" value="DUF1713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal protein mS38 C-terminal" evidence="6">
        <location>
            <begin position="47"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="44"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00035647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="79" mass="9287" checksum="C5A9114127EF048B" modified="2011-11-16" version="1" fragment="single">MMQFGQQPSFIIPAILQQQPCIIAPAQPKIGNLVKDLQENVDEDTMHADSVKRKRKLKMKKHKLRKRRRLQRSLKKRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3APZ4</accession>
    <name>G3APZ4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61391</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375591.1">
        <property type="nucleotide sequence ID" value="XM_007375529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APZ4"/>
    <dbReference type="STRING" id="619300.G3APZ4"/>
    <dbReference type="GeneID" id="18873872"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61391"/>
    <dbReference type="eggNOG" id="KOG0714">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1355412_0_0_1"/>
    <dbReference type="InParanoid" id="G3APZ4"/>
    <dbReference type="OrthoDB" id="442087at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="202" mass="23471" checksum="0377710EF731CFE1" modified="2011-11-16" version="1">MDDLERNLGDNIETLDFSDLLNSLPHQKVQGKSTLKSSKKRPAAEYSDGSSKAETLHTPLNKTTIHAHVPKTTKLTMLDLHASPAVHSFSPPNPPNPVLDPQISAHGWQQYINSIKRYQEEFLEYKRLIVQYQIERTNRDVEHFDLINTNENMDVYTRCLQRDLEVNREFTEALRVFTNTMCVYKQNCDWMRMIKSDTYGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ15</accession>
    <name>G3AQ15_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">6-phosphofructo-2-kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_50901</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375612.1">
        <property type="nucleotide sequence ID" value="XM_007375550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ15"/>
    <dbReference type="FunCoup" id="G3AQ15">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ15"/>
    <dbReference type="GeneID" id="18871497"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50901"/>
    <dbReference type="eggNOG" id="KOG0234">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006383_3_0_1"/>
    <dbReference type="InParanoid" id="G3AQ15"/>
    <dbReference type="OMA" id="KVEPEHQ"/>
    <dbReference type="OrthoDB" id="267323at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF32">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="4">
        <location>
            <begin position="443"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="126"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="269"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="487"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="964"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="19"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="68"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="182"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="522"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="984"/>
            <end position="996"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="996" mass="111443" checksum="78194026EAF3E3B0" modified="2011-11-16" version="1">MSSPITNSPAAPHNNGEIPAVTTEASSSASSADVSSSPSPLSTPSLSHQHHSHHSQPTSESIRKLIASNFNIATSPAQHAGSSSSGNVTPRSRNKSKSISNGVLSHSRTASKKVSFSNFHSINSSQVFTSSSGSSANSSDNDDDDYEDEYTIRNDEGLKKSQTTTSPSQPGFRNVQFTMSVTPSPLTISPTISNSTTNTRGAHKSKSGNALRKFASYGGEKLAMSKSLDSQMLEPCDSTSSVSSLLPEFNKRPLTDTPIVSSMASPVTRYQSSEEDDGAPEHSTIHEEDEEAADNVAPLRKTRNISHLNLSELTEANNIHEEKKLSKTDSDAVEGAAAALASTGAITMPPEFKITNTNFQHTPSASNTPSPSTPTIQMKQEIRDSLLKDVPNEVKEQFEKTPSLEQLKSLLMTKPPPSARKTYTLNIPGQTSSKISPDGKIAQVDVGSKLVIVMVGLPARGKSYITNKLTRYLNWLQHDCRVFNVGNTRRKDKHNAGPEKNPLPDNHTPVNSQSPKEPDHEEAAEEEEEQEHDAAFFSPENKANTALREKWAMDTLDQLLDYVINGTGSVGIFDATNSTKSRRRRVFKRIQERSNGELKVLYLESICNDPNIIESNIRLKLSGPDYKNMDPEVALNDFIGRLHNYEKAYETIDEAEEQIPGFQYVKIIDVGKKIVSYNIQGFLSSQTVYFLLNFNLCERQIWITRHGESRDNLTGRIGGDSHLTKRGDRFARTLAKFMNVKRAEFRKQQLERFSSKLESKYNSMIFNEKDVAMLDSIPTEPNFCVWTSMLIRAIETGQYFNEHLYSVKQMRMLNELGGGKFEGMTYDEIQHKHPREFEARLKDKLSYRYPGVGGESYLDVLTRLRPLITELERTTDHLLIISHRVVLRILLAYFLNLDKSAIGELDVPLHTLYCLESKPYGTDYTMYEYDETLDWFVKSEPDHQKNIKEVGVVFRERKYSVVPTAPPSFRRGGERRPGKHHHNYKEASHSRKPIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ33</accession>
    <name>G3AQ33_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_138901</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375630.1">
        <property type="nucleotide sequence ID" value="XM_007375568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ33"/>
    <dbReference type="STRING" id="619300.G3AQ33"/>
    <dbReference type="GeneID" id="18870160"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138901"/>
    <dbReference type="eggNOG" id="ENOG502RXU0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033727_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQ33"/>
    <dbReference type="OMA" id="IPLFVNH"/>
    <dbReference type="OrthoDB" id="18110at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23524:SF1">
        <property type="entry name" value="MRH DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23524">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04850)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="267"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="315"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="352"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="381"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="442"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="478" mass="52446" checksum="188779E0F61DA8B7" modified="2011-11-16" version="1">MVNHNNYTLLNYLAFNYAGFACISIIVFLSSTQPFYIKEVLELENAKIGGVIGILGFIDELTSMISAPFIGALNDKLNTISGGGSRIVPSIAFLVISIALAGYSVCSKFSELMLFRAIFALGVTGCMSMITVLLNEVGDSDFKLTFKRREILLGHEEEEEEVEAEPERKKNGKFSALIGISTGIGAIFAVSVFVPLPIKLGDKFQLDIKSSLEWAYLVVSGFAIFSFMILIIWLYRTRSNVNSTHENYFELLRHGFEFSRINHQVKLAYAGAFVARSTTVAMAVFIPLMVYDWYYNKGQCKSNDQNTGNLTCHEGYVFSAILTGIAQTIALVSAPFWGFLIDFRKVKSRSTPLKVAALFGLVGNFLFCFSNTTNPKTVTCFLSVCILGVSQIGLIISSMSVLTGISDVHEIMGSLSGFYSFCGGLGILSITLLGGFLSDLWILGPFFILGTFNAALLVICHINRDEKYQGESPLLLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ53</accession>
    <name>G3AQ53_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_61965</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376178.1">
        <property type="nucleotide sequence ID" value="XM_007376116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ53"/>
    <dbReference type="STRING" id="619300.G3AQ53"/>
    <dbReference type="GeneID" id="18874127"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61965"/>
    <dbReference type="eggNOG" id="ENOG502QT9U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_615641_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQ53"/>
    <dbReference type="OMA" id="RPINNGY"/>
    <dbReference type="OrthoDB" id="3598904at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044403">
        <property type="term" value="P:biological process involved in symbiotic interaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070783">
        <property type="term" value="P:growth of unicellular organism as a thread of attached cells"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007618">
        <property type="term" value="P:mating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900428">
        <property type="term" value="P:regulation of filamentous growth of a population of unicellular organisms"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900239">
        <property type="term" value="P:regulation of phenotypic switching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.10:FF:000075">
        <property type="entry name" value="Transcriptional regulatory protein moc3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052360">
        <property type="entry name" value="Transcr_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36206">
        <property type="entry name" value="ASPERCRYPTIN BIOSYNTHESIS CLUSTER-SPECIFIC TRANSCRIPTION REGULATOR ATNN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36206:SF13">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN MOC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0843">Virulence</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="324"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="221"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="364"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="100"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="170"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="266"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="368"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="432"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="445" mass="47828" checksum="484B621FBE9B20B7" modified="2011-11-16" version="1">MTQLPSVSELMTSTKVPSPETTPAQSNHLPAIATTNIDPVNVSPISPVRKITPRSSASSFSNYTSSLPPPVRRLTESSIYSSSGNNSSSSNNNNNNNNNGGNGGAVGGAGSSSIVMGQSSSSESSTQGTRSSFSTLQPPPQIIPGTTASSYPPPHRPINNGYNNTSPLSQQPPPMPMPPLQQGGPSPNGSLPPPPQSHLLPPGVVAQPAYPHYYYYPPPPQMGPPPPQGLEHYYSMAPGQGQLPPQISPPPPGAAYMGQPGGPQGGPLPPQMSPPPPHLTTGYYVSPPGYIPQSLLPPHVAAQYEENNALMNKRRIIKRRTRTGCLTCRKRRIKCDERKPTCFNCERSKKLCLGYENLHNLQPRRRNRDTSLDLSDHDNKPDNEMVDEDEANRRTTKAVSTSSIPTPDGRLSDRGSAESNGVTLPPPIPSYYRRESKVSVHDLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQA1</accession>
    <name>G3AQA1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62014</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376226.1">
        <property type="nucleotide sequence ID" value="XM_007376164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQA1"/>
    <dbReference type="FunCoup" id="G3AQA1">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQA1"/>
    <dbReference type="GeneID" id="18874151"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62014"/>
    <dbReference type="eggNOG" id="ENOG502SGT4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_207409_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQA1"/>
    <dbReference type="OMA" id="DMMAPQL"/>
    <dbReference type="OrthoDB" id="3991365at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020266">
        <property type="entry name" value="Tom6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17112">
        <property type="entry name" value="Tom6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="54" mass="5894" checksum="F5E71C8BA1971DF3" modified="2011-11-16" version="1">MSGRFPTQAPPKEESTFQKIKSTPAFIVGTQAVLFGLGVLFIQSPLMDMLVPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQC6</accession>
    <name>G3AQC6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62043</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376251.1">
        <property type="nucleotide sequence ID" value="XM_007376189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQC6"/>
    <dbReference type="GeneID" id="18874164"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62043"/>
    <dbReference type="HOGENOM" id="CLU_2980578_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQC6"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="58" mass="6852" checksum="9E967AE68F51D667" modified="2011-11-16" version="1">MICRKIKHSQAARGETIESRTLENNRYASGLNPDIRDRNRKCLHYFSISVYAYFMVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQD6</accession>
    <name>G3AQD6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL30m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_62054</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376261.1">
        <property type="nucleotide sequence ID" value="XM_007376199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQD6"/>
    <dbReference type="FunCoup" id="G3AQD6">
        <property type="interactions" value="180"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQD6"/>
    <dbReference type="GeneID" id="18874170"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62054"/>
    <dbReference type="eggNOG" id="ENOG502S7S3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131047_0_2_1"/>
    <dbReference type="InParanoid" id="G3AQD6"/>
    <dbReference type="OMA" id="FHPAEPQ"/>
    <dbReference type="OrthoDB" id="509901at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01658">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005996">
        <property type="entry name" value="Ribosomal_uL30_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01308">
        <property type="entry name" value="rpmD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15892:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15892">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="5">
        <location>
            <begin position="9"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW31483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="95" mass="11182" checksum="54A123C1CF4BB770" modified="2011-11-16" version="1">MSTAKQMYYKITQVRSVIGMPEKKRQILTALGLRKRNYIKYQKVDHRIANQLAMVKELVKVELTDKCQTRQEINQDRKFKPGFELVKGGAFSKYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQE2</accession>
    <name>G3AQE2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Bms1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_140826</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class translation factor GTPase superfamily. Bms1-like GTPase family. BMS1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376267.1">
        <property type="nucleotide sequence ID" value="XM_007376205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQE2"/>
    <dbReference type="FunCoup" id="G3AQE2">
        <property type="interactions" value="1507"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQE2"/>
    <dbReference type="GeneID" id="18870322"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140826"/>
    <dbReference type="eggNOG" id="KOG1951">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002486_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQE2"/>
    <dbReference type="OMA" id="KLHVPMV"/>
    <dbReference type="OrthoDB" id="10260897at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000232">
        <property type="term" value="P:regulation of rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01882">
        <property type="entry name" value="BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000105">
        <property type="entry name" value="BMS1 ribosome biogenesis factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012948">
        <property type="entry name" value="AARP2CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037875">
        <property type="entry name" value="Bms1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007034">
        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030387">
        <property type="entry name" value="G_Bms1/Tsr1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF2">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BMS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08142">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00785">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01362">
        <property type="entry name" value="DUF663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51714">
        <property type="entry name" value="G_BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Bms1-type G" evidence="12">
        <location>
            <begin position="71"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="404"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="554"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1135"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="9"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="470"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="561"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="592"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="636"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="695"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1135"/>
            <end position="1162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1173" mass="134882" checksum="2CD7CC842FD56002" modified="2011-11-16" version="1">MDGQQQANKAHRGGSNKTTAKKKLHQNGQNIKAFAVSAPRKLERMARRTHDVNEKKLHVPMVDRTPDDDPPPVIVAVVGPPGTGKTTLIKSLIRRLTKTSLTEVKGPITVVSGKRRRLTFLECNNDLNSMIDVAKIADLVLLLIDGNYGLEMETMEFLNIAQHHGMPRVLGVATHLDLFKSQSTLRTSKKRLKHRFWTEVYQGAKLFYLSGVINGRYPDREILNLSRFISVMKFRPLKWRNEHPYLLADRITDLTHPQLIADNPKCDRKVAIYGYLHGTPLPSQDAHIHIAGVGDYHVQAVEKLPDPCPTPYFEQKLEELERERAKAAAEAGEPLAKTTRRRKRLEDKQKIIYAPMSDVGGVLVDKDAVYIDIGKESFNREDNEEDMGEGEKLVTDLQEVSKTMQERLEEGPGLQLFSSSKAVHNVNDESEEEEEEGGLLSDLEDDDEVKQNTGRKSLRKARVYGKALNEDDEFDNELSDDEDVVEEQPDMVEVDFGNDRYKENELEFVEDDILSSDDENDYAAAASRLRGSNGRKRWDINKLLYMPNVSPLDAIKRWKGEEDDDEEEEDIEQDDDDFFQKKDVQESSEDLDTLRPKYPSLEELKARFSSEDSDEEYESGYKILKSRLLTAPKMNDDEEQKDEEGDDDEEVYGDFEDLEAEGEDSEQQEDEDEDEEKDEDDFADFEAEEQKEGDSDAEVNDENLSVEEKRKLNAAKKAKLKMQFEEEEYGEDPEGDNEAETWYEFQKNKMAKQLEINKAEFEQLDQATRLKIEGFRAGSYVKLVFNNLPCEFVENFQPEYPIVLGGLLATESRFGIMNVRIRRHRWHKKILKSQDPLILSLGWRRFQTLPIYTTSDSRTRNRMLKYTPEHAYCFASFYGPLVAPNTTFVGFNIVDSRSTTGAFRVAATGIVEDLNSSVEIVKKLKLVGYPYKIFRNTAFVKDMFTNALEVAKFEGAQIRTVSGIRGEIKRALSKPDGHFRATFEDKVLMSDTIFLKTWYPVKVKKFYNPVTSLLLGQHNEWKGMRLTGQVRADANIATPMNNDSAYKKVERSERRFNALKVPKSIRENLPFKSQIHEMKPQKKKTYLSKRAVVLGGDEKKARDLMQKIATVRKEKETKRKTKKDEKFREKLKTIAKKEELRKEKERERKKDYFAKEGKKRGSSEGGQAKRRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQR7</accession>
    <name>G3AQR7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NuA3 HAT complex component NTO1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_141279</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376392.1">
        <property type="nucleotide sequence ID" value="XM_007376330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQR7"/>
    <dbReference type="FunCoup" id="G3AQR7">
        <property type="interactions" value="154"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQR7"/>
    <dbReference type="GeneID" id="18870361"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141279"/>
    <dbReference type="eggNOG" id="KOG0955">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002663_1_1_1"/>
    <dbReference type="InParanoid" id="G3AQR7"/>
    <dbReference type="OMA" id="CMINKNR"/>
    <dbReference type="OrthoDB" id="20839at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15670">
        <property type="entry name" value="ePHD_BRPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15492">
        <property type="entry name" value="PHD_BRPF_JADE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000007">
        <property type="entry name" value="Bromodomain containing 1, isoform CRA_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019542">
        <property type="entry name" value="Enhancer_polycomb-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050701">
        <property type="entry name" value="Histone_Mod_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793:SF107">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10513">
        <property type="entry name" value="EPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="8">
        <location>
            <begin position="233"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="9">
        <location>
            <begin position="287"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="771" mass="88656" checksum="3F9963D0B02F20DA" modified="2011-11-16" version="1">MYSSYTPNNLPNYTSPLDYTLKPREERDFKELYPDLDENEQLPVFISNNEEEIMERQDSNISMITELKRPTFKKSERERPNQNIRFNKQLLDYGFQEPNKPNTKKPLISDTYVRPFQIPTNANAKDSSIDSVEKLLVRKNNLVEYDMDEQDYMFLNDRNSDPKNVVKITPEIFEIMMTTLENEWDKLEHQMNSITSNVGGLNGSDGFEKLLTLGHNNHKYGNDDGIVPGSIYDQKCAVCNDSDCDNSNAIVFCDGCDIAVHQECYGVAFIPEGQWLCRKCMINKNRPTECVFCPSTTGAFKQLDNSLWSHVICGLWINELYFANPIYMEPIEGIDNIPKSRWKLTCYICRQRVGACIQCSNRSCFSAYHVTCAKRAGLYMEMTKGIKGAITNKMTLKTYCERHSPPSYDVDAVLTGIERTRLFFRDTKILNEQNARLSYNQKAENRLNIFKWKTEANTPIAPKLFSDVLMEILLRLKVENQISLPEESTVQVLDLAVLPNRTKQDILEDLRGISDSVCRYWCLKRELKRGAPLVRKNNNLISTSSILYDANSLDVSGFHDESRITEKIKFANILVGDLERLGSMGREMLARQTVLNQLHELEVNNADVIYFPIKSLIELLLNRINAKIDITESLQNYKFRTNEGITLKEIQTRASKYGYESVDEFKQDVEAFIAGVFAENKPASNIYKRMKNWSREFALELPGLEASEKSLLRDGGIFNSSDFCLNGTQSGFKPLDYRKILSENDLSEVEDIESQEDRRLFQEFLGSSENM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQX8</accession>
    <name>G3AQX8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial zinc maintenance protein 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_140167</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Assembly factor required for Rieske Fe-S protein RIP1 incorporation into the cytochrome b-c1 (CIII) complex. Functions as a chaperone, binding to this subunit within the mitochondrial matrix and stabilizing it prior to its translocation and insertion into the late CIII dimeric intermediate within the mitochondrial inner membrane. Modulates the mitochondrial matrix zinc pool.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with RIP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I LYR family. MZM1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375985.1">
        <property type="nucleotide sequence ID" value="XM_007375923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQX8"/>
    <dbReference type="FunCoup" id="G3AQX8">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQX8"/>
    <dbReference type="GeneID" id="18870271"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140167"/>
    <dbReference type="eggNOG" id="ENOG502S6EF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147114_2_2_1"/>
    <dbReference type="InParanoid" id="G3AQX8"/>
    <dbReference type="OMA" id="KYKLRIH"/>
    <dbReference type="OrthoDB" id="529194at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20267">
        <property type="entry name" value="Complex1_LYR_LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045298">
        <property type="entry name" value="Complex1_LYR_LYRM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050435">
        <property type="entry name" value="MZM1/LYRM7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46749">
        <property type="entry name" value="COMPLEX III ASSEMBLY FACTOR LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46749:SF1">
        <property type="entry name" value="COMPLEX III ASSEMBLY FACTOR LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="83"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="83"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="115" mass="13147" checksum="9BC4E167892D4CE3" modified="2011-11-16" version="1">MSSALKAYREALRATRVVFQRDLPRLMAARTEIKTQIKNKANLTDETERLEAINHLEEVTKFLRHNIVQGEVQNNGNVHLNFREETELGDNETIKQGKSEMGSLSGKKGNRIKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR04</accession>
    <name>G3AR04_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62280</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376443.1">
        <property type="nucleotide sequence ID" value="XM_007376381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR04"/>
    <dbReference type="GeneID" id="18874240"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62280"/>
    <dbReference type="HOGENOM" id="CLU_3088794_0_0_1"/>
    <dbReference type="InParanoid" id="G3AR04"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="52" mass="6054" checksum="7C4E565A23841514" modified="2011-11-16" version="1">MDNSVNPEKKRIFSKSLGILTQGVVLKLDSMAFWKFWVCLQIKRTVSQSFTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR09</accession>
    <name>G3AR09_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">High affinity potassium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_67704</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376448.1">
        <property type="nucleotide sequence ID" value="XM_007376386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR09"/>
    <dbReference type="STRING" id="619300.G3AR09"/>
    <dbReference type="GeneID" id="18875176"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67704"/>
    <dbReference type="eggNOG" id="ENOG502QPSA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008142_4_0_1"/>
    <dbReference type="InParanoid" id="G3AR09"/>
    <dbReference type="OMA" id="IWHAVAN"/>
    <dbReference type="OrthoDB" id="504708at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003855">
        <property type="entry name" value="K+_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053952">
        <property type="entry name" value="K_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053951">
        <property type="entry name" value="K_trans_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00794">
        <property type="entry name" value="kup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540:SF83">
        <property type="entry name" value="K+ POTASSIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540">
        <property type="entry name" value="OSMOTIC STRESS POTASSIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02705">
        <property type="entry name" value="K_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22776">
        <property type="entry name" value="K_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="93"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="378"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="431"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="489"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="517"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter integral membrane" evidence="10">
        <location>
            <begin position="59"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter C-terminal" evidence="11">
        <location>
            <begin position="611"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF22776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="717" mass="79890" checksum="62B5C44AA3151338" modified="2011-11-16" version="1">MRNNSQSDLTDRYEIASIEERDDSTISSELSIEDKRSIDTIEHVSPTKKQSWREIFMLSFSSLGVIYGDLGTSPLYTLSSIRYKKLPPTEDEIYGAVSVIFYIFTIIVIIKYVLIVLVIGANNGEGGQVAIYGKIAHQLNIGPKGVTMPGAAREVSDIQLVSRQGTMASSVESYTSRIEHIRKHPRMIVAIKGFILTCCFIGCALVVSDGLLTPTASILSAIGGIQVAVPSFKSVLAVSEVILVVLFSIQQFGSNKISFLFAPIIFIWLIGLMICGIYNIAKYQPGIFAALSPHYAIKILQESGIDVLGGAMLSITGTEAMFADLGHFGRLPIQLTLGCFVYPTLIICYLGQAAYLVHHPDAYINPFFLSLPGGTGGGVYWAVFVFATIATVIASQALILSVFSIFAQLIHLDCFPKLKITHVSSGYAGKVYIGAINWMLMIGVMLTMAGFQNSHRTTAAYGLGISLDLFVTSLLILICMFYVYNWHIIWPILFLLIFGPLEFSLIISNMKKVPRGAWFPIMITVLLSTFLSVWRWCRSRKVEQEFNSKIKIGDLFPYFKKAETIEINLNHKESNQEDSHQFGKTDVKTRFGILHLTRHEGLGIMYVDSILTNSPNTLPDLYTKLVTTFVSIPSHFVFCAIRVMSIPFVPDEQRLFVAPMKLPDHYKCVIRFGYMELTRIDKAMISLIMSKIPALSGLNEDQLEKYPKLHIFENDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR99</accession>
    <name>G3AR99_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleolar GTP-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_62311</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376484.1">
        <property type="nucleotide sequence ID" value="XM_007376422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR99"/>
    <dbReference type="FunCoup" id="G3AR99">
        <property type="interactions" value="1335"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR99"/>
    <dbReference type="GeneID" id="18874266"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62311"/>
    <dbReference type="eggNOG" id="KOG1490">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011784_4_1_1"/>
    <dbReference type="InParanoid" id="G3AR99"/>
    <dbReference type="OMA" id="ILEHDEW"/>
    <dbReference type="OrthoDB" id="415015at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01897">
        <property type="entry name" value="NOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000496">
        <property type="entry name" value="Nucleolar GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010674">
        <property type="entry name" value="NOG1_Rossman_fold_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012973">
        <property type="entry name" value="NOG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45759">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06858">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08155">
        <property type="entry name" value="NOGCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="OBG-type G" evidence="9">
        <location>
            <begin position="33"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="443"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="461"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="507" mass="57960" checksum="232F9420C1E97F2F" modified="2011-11-16" version="1">MATITKKLKDAFIYLEQVRQHLGRLPSIDPNTRTLVICGYPNVGKSSFLRCITKADVEVQPYAFTTKSLYVGHFDYKYLRFQAIDTPGILDRPTEEMNNIEMQSIYAIAHLRSCVLYFMDLSEQCGFSIEAQVKLFHSIKPLFANKSVMVVMNKSDIIKVEDLDAERQELLKSISTVPGVEIMDASCHEEENVMKVRNHACEKLLTARIEQKLKGTARVNNVLNKIHVAKPQARDDVDRAPYIPDAVKELGKYDVNDPNRRKLARDIEAENGGAGVFNINLKDKYLLEDEDWKNDVMPEFLDGKNVYDFLDPDIAAKLQALEDEEERLEQEGFYDSDSDIEDEETEEIREKADWIRNKQKTMIIEARNRKSLKNKAIMPRDQVKKSFGEMEEHMYNIGHDTDKLREKVGKGKKNEGKALSGVDILKKNQGIQASRLAKKKMAAHQSDRLNDGLADGALRSQAERLAKIQRRERNRMARQGEGDRHSTAMLPKHLFSGKRGIGSTDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARB6</accession>
    <name>G3ARB6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_56512</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376501.1">
        <property type="nucleotide sequence ID" value="XM_007376439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARB6"/>
    <dbReference type="FunCoup" id="G3ARB6">
        <property type="interactions" value="1147"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARB6"/>
    <dbReference type="GeneID" id="18872042"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56512"/>
    <dbReference type="eggNOG" id="KOG3117">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065858_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARB6"/>
    <dbReference type="OMA" id="PVHYNET"/>
    <dbReference type="OrthoDB" id="203440at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007146">
        <property type="entry name" value="Sas10/Utp3/C1D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237:SF9">
        <property type="entry name" value="NEUROGUIDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04000">
        <property type="entry name" value="Sas10_Utp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="319" mass="36204" checksum="80B468541612F3AC" modified="2011-11-16" version="1">MSNLDTLLNDIITSVKATKSSIDDIKDHLISSLLEKTSTTNVEGVSLLSLKNQALLSYINNIALVVLGQVARLEEVDDVQLWEDSVKRSIVQRVTLEKGIKPLEKKIGYQLDKMVRAYTRMEEDERKIEDKLNKADNSDENSDEDEEDSEEEDDRLAYRPDASALAKLAPKGKDTKPSGDEKYVAPKISAMAPPSAIPKEKPTKTKKLQSMEEYLQEQSEMPTAEASIGSTIVDHGRGGVKTQHDRRKEQEIQTYEESNFIRLPQAQTKKSFKQKQRDMANQFAGEDWAMFNNDRQVSSGTSRKRKPTSVWDRVKKRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARQ3</accession>
    <name>G3ARQ3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UDP-galactose transporter homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_62399</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleotide-sugar transporter family. SLC35B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376584.1">
        <property type="nucleotide sequence ID" value="XM_007376522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARQ3"/>
    <dbReference type="FunCoup" id="G3ARQ3">
        <property type="interactions" value="470"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARQ3"/>
    <dbReference type="GeneID" id="18874310"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62399"/>
    <dbReference type="eggNOG" id="KOG1581">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036019_0_2_1"/>
    <dbReference type="InParanoid" id="G3ARQ3"/>
    <dbReference type="OMA" id="CGAIGQV"/>
    <dbReference type="OrthoDB" id="1601at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005459">
        <property type="term" value="F:UDP-galactose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005460">
        <property type="term" value="F:UDP-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120112">
        <property type="term" value="P:UDP-glucose transmembrane transport into endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013657">
        <property type="entry name" value="SCL35B1-4/HUT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08449">
        <property type="entry name" value="UAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="193"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW31806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="356" mass="40198" checksum="9302D1EE1181F9FA" modified="2011-11-16" version="1">MTSTIVKPTDTKLQPNKNMKQRGRVITLITCVLGLYGTFLTWSILQERINTKPYGDKQEYFQSPLIINIIQALCASLVGFIYTIFANNSSPFDVFFKNDYESSMSIFKSLVIISITSSLASPLGYKSLNHLDYVAYLLAKSSKLIPVMFIHFVFYRTKFPWYKYLVAVLITLGVIAFTISHGAKKTSINDGNSLYGMSLLFGSMLLDGLTNSTQDQLFKQKFKVRLTGAKLMCLLNLFVFIISLSYTLIFQFDEVKEATLFIHKHHELVVDIISFSLCGAIGQIFVFIILEKFDSIVLITATVTRKMLSMILSVILFGHTLNVTQWIGVLLVFGGIGYESMIKFNGKKVSVQKKME</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARR6</accession>
    <name>G3ARR6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Nuclear protein STH1/NPS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_154321</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376111.1">
        <property type="nucleotide sequence ID" value="XM_007376049.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3ARR6">
        <property type="interactions" value="1067"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARR6"/>
    <dbReference type="GeneID" id="18870976"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_154321"/>
    <dbReference type="eggNOG" id="KOG0386">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1827">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_15_3_1"/>
    <dbReference type="InParanoid" id="G3ARR6"/>
    <dbReference type="OMA" id="VNYISHT"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015616">
        <property type="term" value="F:DNA translocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140054">
        <property type="term" value="F:histone H2A reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140015">
        <property type="term" value="F:histone H3K14ac reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140008">
        <property type="term" value="F:histone H4 reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17996">
        <property type="entry name" value="DEXHc_SMARCA2_SMARCA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000008">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000843">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029295">
        <property type="entry name" value="SnAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14619">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01314">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSA" evidence="14">
        <location>
            <begin position="321"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="504"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="815"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="11">
        <location>
            <begin position="1197"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="433"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1120"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1120"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1152"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1163"/>
            <end position="1172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW31333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1289" mass="149339" checksum="43319457396217DA" modified="2011-11-16" version="1">MNGVMTINGNGPEVTPPAGQVPAPPSDNQELVSFGYTFPQTKEQLNDLLDRYYKLLYASKQLREEFEKRQDSPGLKFEDPNSKQKHQIEAILAKVSKAQDEFLQEEQEFLNELASTEVDQEQIDQFDGKLFEKQIAGLLLLSKDVDLPEPLQREFSNQIKKHAISVDSIIERLITIDNDYETRAESLGIKVPLPLSAIDPHYAPLGNFYAEKIINNKISQRLKDLENLPSNLGTYHTDKEEAELDNLKIKALIEIKALRLLTKQKQLKRNILVHESQQAKFTHPNLKDHPIILSEKRSFNVRPKVEQRHPELLAIQLEELKKQEEKELKRQMHISKVEQILESSLALGNAKYQRDHYRSHNLVRQINNFHQNTEKEESKKLEKTAKQRLQALKANDEEAYIKLLDQTKDHRITHLLKQTNTFLDSLAQQVRAQQQEDEEFPMEKSGSPESAPETKPSDANDELREKIDYYEVAHRVKEVITEQPSILVGGKLKEYQIKGLQWMVSLYNNKLNGILADEMGLGKTIQSISLVTYLIEKKHEEKFLVIVPLSTITNWTLEFEKWAPSVKLVVYKGSQQQRRSMQPEIRVGNFQVLLTTYEYIIRERPLLCKFHYSHMIIDEGHRMKNAHSKLSQTLRTYYKTKNRLILTGTPLQNNLPELWALLNFVLPRIFNSVKSFDEWFNTPFANTGAQEKIELTEEESLLVIRRLHKVLRPFLLRRLKKDVEKDLPDKVEKVLKCNLSGLQYVLYQQMLKHNALFVGADVGGAKSGIKGLNNKIMQLRKICNHPFVFEEVEAVLNSSRLTNDLIWRVSGKFELLDRVLPKFKASGHRVLMFFQMTQVMDIMEDFLRWRDMKYLRLDGSTKAEDRQDMLKVFNAPDSDYFCFLLSTRAGGLGLNLQTADTVVIFDTDWNPHQDLQAQDRAHRIGQKNEVRILRLITNDSVEEVILERAHQKLDIDGKVIQAGKFDNKSTAEEQEAFLKRLLEAEANEDNEENDSLDDEELNEILARSDAEKVLFNQMDEERKKADKSIGSRLIEKDELPSVFTEDISQHFEKDTKELSRMREKKRVKYDDGLTEEQWLMAMDDDEDTVEDAIKRKEARIAKRKRNKAIREGLLEEDIKDDEVGEEDDDDYEAVAAPRKRQRRSRTPLPHISEPEESNRNSDEDGEIDGQIDGEVDEFTGKCLAVIDEIMELTDPADGHNLADIFLKLPSRKLYPDYYQIIKRAVSINQVKKQLEQERFGSFEEFIGELKQMCLNAKTYNEEGSFVYSDAVVIENLLDEKLAQDAQDQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS13</accession>
    <name>G3AS13_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">fumarate hydratase</fullName>
            <ecNumber evidence="2">4.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_62472</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reversible stereospecific interconversion of fumarate to L-malate. In mitochondrion, catalyzes the hydration of fumarate to L-malate in the tricarboxylic acid (TCA) cycle to facilitate a transition step in the production of energy in the form of NADH. In cytoplasm and nucleus, involved in DNA repair in response to DNA damage: following DNA double-strand breaks (DSBs), translocates from the cytosol to the nucleus and promotes DNA repair by catalyzing the dehydration of L-malate to fumarate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.2" evidence="2"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376640.1">
        <property type="nucleotide sequence ID" value="XM_007376578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS13"/>
    <dbReference type="STRING" id="619300.G3AS13"/>
    <dbReference type="GeneID" id="18874335"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62472"/>
    <dbReference type="eggNOG" id="KOG1317">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021594_4_1_1"/>
    <dbReference type="InParanoid" id="G3AS13"/>
    <dbReference type="OMA" id="AKWRAQT"/>
    <dbReference type="OrthoDB" id="1738025at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004333">
        <property type="term" value="F:fumarate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006106">
        <property type="term" value="P:fumarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01362">
        <property type="entry name" value="Fumarase_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000002">
        <property type="entry name" value="Fumarate hydratase class II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.10:FF:000001">
        <property type="entry name" value="Fumarate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000001">
        <property type="entry name" value="Fumarate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00743">
        <property type="entry name" value="FumaraseC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005677">
        <property type="entry name" value="Fum_hydII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018951">
        <property type="entry name" value="Fumarase_C_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00979">
        <property type="entry name" value="fumC_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11444">
        <property type="entry name" value="ASPARTATEAMMONIA/ARGININOSUCCINATE/ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11444:SF1">
        <property type="entry name" value="FUMARATE HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10415">
        <property type="entry name" value="FumaraseC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="5">
        <location>
            <begin position="13"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarase C C-terminal" evidence="6">
        <location>
            <begin position="380"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00056821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="436" mass="46730" checksum="811251C380EBB5EA" modified="2011-11-16" version="1">MNFKIGGTAARMPTPVIRAFGVLKKSTAIVNEQLGALDPKLSAAIQQAATEVAEGKLDDHFPLVVFQTGSGTQSNMNANEVISNRAIEILGGELGSKKPVHPNDHCNMSQSSNDTFPTVMHIAATTEIYNSLLPELTKLRDALQAKSEEFKDIIKIGRTHLQDATPLTLGQEFSGYAQQLTNGIERVEKVLPNLLFLAQGGTAVGTGLNTRKGWDVKVAEQVSKLTGFPFKTAPNKFEALAAHDAIVEASGALNTVAVSLYKIANDIRYLGSGPRCGYGELALPENEPGSSIMPGKVNPTQNEALTMVACQVFGNNAAITFAGASGQFELNVFKPVMIANLLSSIRLIADGSASFRTNCVEGIKANKDKIEKTLHESLMLVTALNPKIGYDAASKTAKNAHKKGLTLKQSALELGVLSEQEFDEWVRPEKMIGPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASH9</accession>
    <name>G3ASH9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">methionine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Methionyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_142477</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="3"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377130.1">
        <property type="nucleotide sequence ID" value="XM_007377068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASH9"/>
    <dbReference type="FunCoup" id="G3ASH9">
        <property type="interactions" value="1082"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASH9"/>
    <dbReference type="GeneID" id="18870451"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142477"/>
    <dbReference type="eggNOG" id="KOG1247">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009710_4_1_1"/>
    <dbReference type="InParanoid" id="G3ASH9"/>
    <dbReference type="OMA" id="HLNTTEY"/>
    <dbReference type="OrthoDB" id="5844513at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017102">
        <property type="term" value="C:methionyl glutamyl tRNA synthetase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.20:FF:000001">
        <property type="entry name" value="Methionine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000037">
        <property type="entry name" value="Methionyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.20">
        <property type="entry name" value="Methionyl-tRNA synthetase, Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00098">
        <property type="entry name" value="Met_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018285">
        <property type="entry name" value="Met-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023458">
        <property type="entry name" value="Met-tRNA_ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029038">
        <property type="entry name" value="MetRS_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001100">
        <property type="entry name" value="PRK00133.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765">
        <property type="entry name" value="METHIONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09635">
        <property type="entry name" value="MetRS-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57770">
        <property type="entry name" value="Methionyl-tRNA synthetase (MetRS), Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methionyl-tRNA synthetase N-terminal heteromerisation" evidence="14">
        <location>
            <begin position="13"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Leucyl tRNA synthetase" evidence="13">
        <location>
            <begin position="189"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl-tRNA synthetase anticodon-binding" evidence="15">
        <location>
            <begin position="612"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF09635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF19303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW31097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="751" mass="84489" checksum="F44944C65F65B809" modified="2011-11-16" version="1">MSSLEIFGAKGSSLLTLSTNLKLCIATSAFQPKLSVSNDTPSDALLKDKKSDFALHEVNAVVKYLANDFSTNEAIQFEETQLYKALKSNKKEKLTSAVSGSITFTKAELTPAQIILFASVYPVLKDAPTAWFTEFQAIPQVVAGIAAALAITTLEREKETNTGTQRVVVGHLVRPQEKAIQPKEGERNILVTSALPYVNNVPHLGNIIGSVLSADIYARYAKNRNYNCLYVCGTDEYGTATETKALEEKVTPQQLCDKYHAIHKSVYDWFDIGFDYFGRTTTQAQTDIAQDIFLKLHKGGFLEEKTTEQLYCQEHKAFLADRFVEGTCPKCAYEDARGDQCDKCGNLLDPLELINPRCKVEGATPVVKESTHIYLKLNELEEPLAKWVAHSEEKGHWSKNSRTITNSWLKQGLEPRCITRDLIWGTPVPLEGYDEKVLYVWFDACIGYVSITSNYFKERSTQQDWEKWWRNPENVDLYQFMGKDNVPFHTVVFPSSQIGTEIAAGDKWTKLHHLNTTEYLQYEGGKFSKSRNIGVFGNNAADTGVPASVWRYYLASVRPESSDSQFSWDEFVTKNNSELLANLGNFVNRIVKFAIAKYNGVIPKFNTSAIPEYEAFESEINALLAQYIETMEAVNLRKGLELAMHISSRGNQFLQDNKLDNSLYANQPDKSDAVVAVALNLVYLVSAIIYPFMPETTVKIDEILNVPALSITDKFELVLEPGHCIGKAQYLFTRIDEKKIEEWRGKYGGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASP8</accession>
    <name>G3ASP8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_142784</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GOT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377145.1">
        <property type="nucleotide sequence ID" value="XM_007377083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASP8"/>
    <dbReference type="FunCoup" id="G3ASP8">
        <property type="interactions" value="840"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASP8"/>
    <dbReference type="GeneID" id="18870485"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142784"/>
    <dbReference type="eggNOG" id="KOG1743">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124519_1_0_1"/>
    <dbReference type="InParanoid" id="G3ASP8"/>
    <dbReference type="OMA" id="MWLTDAQ"/>
    <dbReference type="OrthoDB" id="204784at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000137">
        <property type="term" value="C:Golgi cis cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045176">
        <property type="entry name" value="Got1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493">
        <property type="entry name" value="CGI-141-RELATED/LIPASE CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493:SF9">
        <property type="entry name" value="GOLGI TRANSPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="140" mass="15463" checksum="B4D2C886496769C0" modified="2011-11-16" version="1">MIWLSERQKFGVGFTAGGIFFFFFGIITFFDSGFLALGNILFILGLILIIGPQRTISFFTRPTKIRGTICFVLGILLILVKHPFVGFVLESFGILGLFGDFFGTIVQFLRSIPYIGDVLSHPLIAPTIDRLAGITDVLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASQ3</accession>
    <name>G3ASQ3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Non-structural maintenance of chromosomes element 1 homolog</fullName>
            <ecNumber evidence="1">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_156828</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the Smc5-Smc6 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NSE1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="1"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377150.1">
        <property type="nucleotide sequence ID" value="XM_007377088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASQ3"/>
    <dbReference type="FunCoup" id="G3ASQ3">
        <property type="interactions" value="135"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASQ3"/>
    <dbReference type="GeneID" id="18871059"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_156828"/>
    <dbReference type="eggNOG" id="KOG4718">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045153_4_0_1"/>
    <dbReference type="InParanoid" id="G3ASQ3"/>
    <dbReference type="OMA" id="RCPNYSN"/>
    <dbReference type="OrthoDB" id="185455at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011513">
        <property type="entry name" value="Nse1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20973">
        <property type="entry name" value="NON-SMC ELEMENT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20973:SF0">
        <property type="entry name" value="NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07574">
        <property type="entry name" value="SMC_Nse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU368018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="240" mass="27980" checksum="3E7884A072133194" modified="2011-11-16" version="1">MSEYTDIHRVLLTYIRSCKYIYHHELLETFQTIYTNLSGESPHEEPLQQLNKYLADINSRISPHGFKIERKNNELTGELCYIFINTVGDDIIKQNTLYSPPELDTIKQIIDDIVEAPEYKFSLGKVNVQQKIANSLNKTLKEASNLVDRLIDDGWFEVTLDDRMTLSIKTIAELKDYLIDRHGSSTNEGKILICQQCKEIVTLGCKHEDYFFHFKCYDVYCRNNNIEPDQDFQRIGPENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASU2</accession>
    <name>G3ASU2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP phosphoribosyltransferase</fullName>
            <ecNumber evidence="4">2.4.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_63070</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of the enzymatic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + ATP</text>
            <dbReference type="Rhea" id="RHEA:18473"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="2.4.2.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATP phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.17" evidence="4"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377188.1">
        <property type="nucleotide sequence ID" value="XM_007377126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASU2"/>
    <dbReference type="FunCoup" id="G3ASU2">
        <property type="interactions" value="201"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASU2"/>
    <dbReference type="GeneID" id="18874581"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63070"/>
    <dbReference type="eggNOG" id="KOG2831">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038115_1_2_1"/>
    <dbReference type="InParanoid" id="G3ASU2"/>
    <dbReference type="OMA" id="ITAKKYV"/>
    <dbReference type="OrthoDB" id="2574at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003879">
        <property type="term" value="F:ATP phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000123">
        <property type="entry name" value="HIS1p ATP phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013820">
        <property type="entry name" value="ATP_PRibTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018198">
        <property type="entry name" value="ATP_PRibTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001348">
        <property type="entry name" value="ATP_PRibTrfase_HisG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013115">
        <property type="entry name" value="HisG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00070">
        <property type="entry name" value="hisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03455">
        <property type="entry name" value="HisG_C-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403:SF8">
        <property type="entry name" value="ATP PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403">
        <property type="entry name" value="ATP PHOSPHORIBOSYLTRANSFERASE ATP-PRTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01634">
        <property type="entry name" value="HisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08029">
        <property type="entry name" value="HisG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01316">
        <property type="entry name" value="ATP_P_PHORIBOSYLTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP phosphoribosyltransferase catalytic" evidence="11">
        <location>
            <begin position="59"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine biosynthesis HisG C-terminal" evidence="12">
        <location>
            <begin position="224"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="284" mass="31173" checksum="FF57F4021193B7D8" modified="2011-11-16" version="1" fragment="single">MDLVNHLPDRLLFAVPKKGRLYEKCCNLLDGADVKFRRSNRLDIALSTNLPVALIFLPAADIPIFVGEGNCDLGITGLDQIKEAEMFDNIEDLLDLQFGSCKLQIQVPAAGEYTKPEQLIGKKIVSSFTKLSNNYFRDLEGKTNDDALTTNIRYVGGSVEASCALGVADAIVDLVESGETMKAAGLKPIETILETSAHLISSKTPRFPEIVEKIHQRFEGVLTAQKYILCNYNAPKTIVSKCLQITPGRRAATVSSLDKHLADDEDWVAISAMVERKTIGDVMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT10</accession>
    <name>G3AT10_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PPPDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_63086</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377203.1">
        <property type="nucleotide sequence ID" value="XM_007377141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT10"/>
    <dbReference type="STRING" id="619300.G3AT10"/>
    <dbReference type="GeneID" id="18874588"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63086"/>
    <dbReference type="eggNOG" id="KOG0324">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101028_0_1_1"/>
    <dbReference type="InParanoid" id="G3AT10"/>
    <dbReference type="OMA" id="HLMLGKQ"/>
    <dbReference type="OrthoDB" id="21221at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070646">
        <property type="term" value="P:protein modification by small protein removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF7">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="4">
        <location>
            <begin position="16"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW31170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="178" mass="20204" checksum="504F100B6628878D" modified="2011-11-16" version="1">MTTETPGQEEQAEQEYPVKVYVYDLSHGLASLYSPMILGKSIPAIYHTSVVIRNREYYLDQGIKSHSPPGSTRFGVPIEIIDMGTTSIDEDILQEFIEDLKNHEDMKYHAISYDLFRNNCNHFTDVMVEFLVGKNLEDRILKLPELVLSTGNGQLLQQIIYGGSPAFGNSGQWQNPYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT19</accession>
    <name>G3AT19_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Secretory lipase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_142286</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:12045"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377212.1">
        <property type="nucleotide sequence ID" value="XM_007377150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT19"/>
    <dbReference type="GeneID" id="18870437"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142286"/>
    <dbReference type="eggNOG" id="ENOG502S2P7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029538_5_0_1"/>
    <dbReference type="InParanoid" id="G3AT19"/>
    <dbReference type="OMA" id="SAWAAEM"/>
    <dbReference type="OrthoDB" id="2373480at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005152">
        <property type="entry name" value="Lipase_secreted"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34853">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34853:SF1">
        <property type="entry name" value="LIPASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03583">
        <property type="entry name" value="LIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029171">
        <property type="entry name" value="Esterase_LipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013435477" evidence="4">
        <location>
            <begin position="17"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR029171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW31179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="459" mass="49906" checksum="8D1806D3A05F6155" modified="2011-11-16" version="1">MPFIIFLFLFILQAIAIPLQLKSPLDDDFYTLPQGYEAAAPGDILKFRPAPAQLASLFISIKVKNVWQLVVRSEDSFGQPTAFVTTIIEPFNADPSKVVSYQTMEDSASLNCSPSYGLQSGAPISTIATQYDMTFMMIALENGYFVASPDYEGFISAFTVGRRSGQAVLDSVRAVIKSGNFTGIKKDAKVGLWGYSGGALASGWAALLQPSYAPELKSTLVGASLGGFVTNITAVAEIVDGELFAGLIPVALTGVSNEYPVAKTLIAENIAPQHLAKFNEAGNKCLIPSVLYFFNDHILTGKDPMFPAGFSLLQNGQVKEIVEENSIVYLDSSYVPKIPIFVYHGTLDKVVPIKDVYVTYDNWCQWGINSFEFAEDTLNGHISEQLVGGPASWTWLNGIFQGNNPVSGCSHTKRVSNLLYPGISSATKQYFEGIKDSLLKVKLGPDAKNMTVIDIMNKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATC4</accession>
    <name>G3ATC4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein BIG1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_62789</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BIG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376920.1">
        <property type="nucleotide sequence ID" value="XM_007376858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATC4"/>
    <dbReference type="FunCoup" id="G3ATC4">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATC4"/>
    <dbReference type="GeneID" id="18874466"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62789"/>
    <dbReference type="eggNOG" id="ENOG502RXHV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067894_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATC4"/>
    <dbReference type="OMA" id="PNWNPIR"/>
    <dbReference type="OrthoDB" id="9985059at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037654">
        <property type="entry name" value="Big1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28285">
        <property type="entry name" value="PROTEIN BIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28285:SF1">
        <property type="entry name" value="PROTEIN BIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442696" description="Protein BIG1" evidence="11">
        <location>
            <begin position="19"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW30887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="320" mass="37014" checksum="DE81AAE5272BFC53" modified="2011-11-16" version="1">MKIQQLLITVYIFALASASLAPLLVASHKLVPGLKQLINPSNIEPHNVTSVTNLLKKLVTECSSDAYLIVNQPGLTYQDLITANKDHWPMLRKYLYMSSTTVGIPRIDGVIDLQFLQDYIVNTCEAEVINVWNEDEGEVVDYFDIRKRVIRIDLGELPTGSERNLEILNHDELLRKILRKLPSPHYTIILTSDSPGVIHPVPGVHMAEFPDRFEIFNDIINDPMHSREIEKNNNFHSVEPNWNPIRNSNDRYIRNKHNDEVHLLDYDLWTKNEKLISTLIVMILSIFMMKILQFFNYLKKRIVSYKQNKNTKGIIGKKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATD5</accession>
    <name>G3ATD5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Vacuolar calcium ion transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_62809</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Has a role in promoting intracellular calcium ion sequestration via the exchange of calcium ions for hydrogen ions across the vacuolar membrane. Involved also in manganese ion homeostasis via its uptake into the vacuole.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Vacuole membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376931.1">
        <property type="nucleotide sequence ID" value="XM_007376869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATD5"/>
    <dbReference type="FunCoup" id="G3ATD5">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATD5"/>
    <dbReference type="GeneID" id="18874473"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62809"/>
    <dbReference type="eggNOG" id="KOG1397">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008721_2_1_1"/>
    <dbReference type="InParanoid" id="G3ATD5"/>
    <dbReference type="OMA" id="AVMITCN"/>
    <dbReference type="OrthoDB" id="1699231at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015369">
        <property type="term" value="F:calcium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000011">
        <property type="entry name" value="Vacuolar calcium ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000021">
        <property type="entry name" value="Vacuolar calcium ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004713">
        <property type="entry name" value="CaH_exchang"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004798">
        <property type="entry name" value="CAX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00846">
        <property type="entry name" value="caca2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00378">
        <property type="entry name" value="cax"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503:SF22">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0050">Antiport</keyword>
    <keyword evidence="6 10" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 10" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="130"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="207"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="345"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="378"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="406"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="11">
        <location>
            <begin position="68"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="11">
        <location>
            <begin position="281"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW30898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="431" mass="46540" checksum="4E22FDB01D9A02EF" modified="2011-11-16" version="1">MSDLPLLSTRRSSSPFVNPDDSYLKQFLTTTWWTTKLTFKSSPVNYLLVFLPLGIAAGELNWSSNARFWLNFVAIIPLASILAYATEEISDFVGQTAGGLMNATFGNAVELIVSIIALKENQVRIVQASMLGSILSNLLLVLGCCFIAGGITRVQQTFNQTVAQTMSSLMALATASLLIPAAFHASLPASGGKHVGFKPAPGNGDDLILSLSRGVSVILLIVYVLYLFFSLKTHKALFEGEEEDHNVIRTVSDNDNVLTLTPVTSNKLRHNHETHLTVTGSIIVLLAATVLVSISADYLVGSIDEIVETTGLSKTFIGLIVIPIVGNAAEHVTAIIVAMKDKMDLAIGVAVGSSLQIANFVTPFMVLIGWAIDVPMSLYFSTFETAILFVSVFITNLVILDGESNWLEGAMLLSMYLIAALAFFYHPDEML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATI7</accession>
    <name>G3ATI7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SUN-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_156436</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="9">Interacts with EMP65.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the SLP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376983.1">
        <property type="nucleotide sequence ID" value="XM_007376921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATI7"/>
    <dbReference type="FunCoup" id="G3ATI7">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATI7"/>
    <dbReference type="GeneID" id="18871050"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_156436"/>
    <dbReference type="eggNOG" id="KOG1396">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006633_4_0_1"/>
    <dbReference type="InParanoid" id="G3ATI7"/>
    <dbReference type="OMA" id="YVIIELC"/>
    <dbReference type="OrthoDB" id="266334at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000099">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045120">
        <property type="entry name" value="Suco/Slp1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12953">
        <property type="entry name" value="MEMBRANE PROTEIN CH1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12953:SF0">
        <property type="entry name" value="SUN DOMAIN-CONTAINING OSSIFICATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51469">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="637"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="SUN" evidence="13">
        <location>
            <begin position="272"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="439"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="665"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="107"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="259"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="451"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="684"/>
            <end position="694"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW30950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="694" mass="76100" checksum="B5344AB893B51AA8" modified="2011-11-16" version="1">MESSSETFASETSSDISSSSEVSSSEISSSSEISSSLESSSSMSSSSSSEISSSSVTSSSEISSSSEISSSSEISSSSEISSSSEISSTEHESSSFEPSSTEHESSSFEPSSTEAESSSAEPSSSSEIESSSITDFEASSSLEPSSSEVSSTEEPSSSEASESSETSSESSSSSSSSSSSSSSSSSSSSSNNTIIDNVHFLSFEEWKKQKIIEKNNHTSASSISRILSSSSSSSSSISTASSISSSKCISANNTCSTQSLNNTSTASNSPNGTTIEEDASAKPIKETEGKVYKDKFNYASVDCAATIVKTNANAKSPSAILKENKDSYLLNQCSIPNKFVVIELCQDILVDSVVIGNFEFFSSMFKEIRISVSDRFPTSNWKVLGEFTAKNIRDVQSFKIENPLIWARYLRLEILSHYGSEFYCPISVVRVHGKTMMEEFKEDTEQQQQQEQKEQEQQQEKEKPELFSKEEPQMTNILMLNQTGNECPIIMPHLKLNAFLKDINQTQDYCLPQSEPITSTAIPTTQESIYKNIMKRLSLLESNATLSLLYIEEQSKLLSTAFSNLEKRQTANFNALISSVNVTLINQLTSFKEAFNSLHDQYGNLYETQLQSYHHLLQDSNKKVSTLTSELTFQKRVTVFNSIIIICLLVYVILTRDTYIDVQQDDETEGKVSTPRSTPFKKFTPTKRNKYKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATM4</accession>
    <name>G3ATM4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Golgi apparatus membrane protein TVP18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Golgi apparatus membrane protein tvp18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_62891</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Golgi membrane protein involved in vesicular trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TVP18 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377020.1">
        <property type="nucleotide sequence ID" value="XM_007376958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATM4"/>
    <dbReference type="FunCoup" id="G3ATM4">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATM4"/>
    <dbReference type="GeneID" id="18874513"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62891"/>
    <dbReference type="eggNOG" id="ENOG502S3AC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118698_1_0_1"/>
    <dbReference type="InParanoid" id="G3ATM4"/>
    <dbReference type="OMA" id="IYAQWLG"/>
    <dbReference type="OrthoDB" id="5591789at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019365">
        <property type="entry name" value="TVP18/Ca-channel_flower"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13314">
        <property type="entry name" value="CALCIUM CHANNEL FLOWER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13314:SF2">
        <property type="entry name" value="CALCIUM CHANNEL FLOWER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10233">
        <property type="entry name" value="Cg6151-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01077">
        <property type="entry name" value="Cg6151-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="173" mass="19296" checksum="FB9E87021328F469" modified="2011-11-16" version="1">MALSVGSIFTNIFGGLSQDFKKKNFSLYGQWIALITILLCLALGVANIFHFNLVVIFSVICLVQGLIVVFVEMPFLLKICPLTDTFVNFVRRFDDNLPRCGFYLLNAVIQWLSLTLQATSLIVVAVFFTLASACYALAYFKHQEFMKSTLDVTGNGNSDLEAQVGQHVIRNVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATV7</accession>
    <name>G3ATV7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_52427</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377305.1">
        <property type="nucleotide sequence ID" value="XM_007377243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATV7"/>
    <dbReference type="STRING" id="619300.G3ATV7"/>
    <dbReference type="GeneID" id="18871679"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52427"/>
    <dbReference type="eggNOG" id="ENOG502QVCV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093528_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATV7"/>
    <dbReference type="OMA" id="YLEEVVW"/>
    <dbReference type="OrthoDB" id="272977at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF27">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN CTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0186">Copper</keyword>
    <keyword evidence="5" id="KW-0187">Copper transport</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="222" mass="24993" checksum="FFB066A2A26B2699" modified="2011-11-16" version="1">MSFQNKHMMMSMDSDTATATMDMVMETASSAMDHMSSHDDMAGMHMYFTAQFKGYPVIFKNLVANNKGQAFGIFLLLFFVAFLNRLLEFVRNYLEEVVWQNREYQEFESGIANAPANLQSVTSGANCHDTNSSDDSLHKGDSVQVSKPVKSLPIASTLFRDAIRLFLCIVPDLFGYALMLGAMTFTLTYFFAIVIGSGVGRFTSERLMERFRLKRTPPLNCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATX1</accession>
    <name>G3ATX1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_52440</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377318.1">
        <property type="nucleotide sequence ID" value="XM_007377256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATX1"/>
    <dbReference type="STRING" id="619300.G3ATX1"/>
    <dbReference type="GeneID" id="18871682"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52440"/>
    <dbReference type="eggNOG" id="ENOG502QU6M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021993_1_0_1"/>
    <dbReference type="InParanoid" id="G3ATX1"/>
    <dbReference type="OMA" id="AHGSYGV"/>
    <dbReference type="OrthoDB" id="413079at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000286">
        <property type="entry name" value="MFS efflux transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051788">
        <property type="entry name" value="MFS_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23514">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23514:SF6">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043758202" description="Major facilitator superfamily (MFS) profile domain-containing protein" evidence="6">
        <location>
            <begin position="20"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="336"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="365"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="408" mass="45165" checksum="441A41C0FDDC7C2D" modified="2011-11-16" version="1">MFRLLTALFWAFGSGLSDGSVGVLLNYIEIQYRISYSVASILWLSNASGCILVALLSDVIQKKLGRKSIFWGCVSCIIMYTLVSTGSYYPIIVIGFFFYGMGAGICVAQYNVFIGRLEKSSTALGYFHGCYGLGASASPLIATAFVEAGLKWNYYYLVVLGIFLISTVNTYFAFVHGQEDLNPWDEKEQQQQQPVLQVKSTGLMKEALTNKRTWFSALFIFFYQGGEVSLGGWITTYLRDYRNHKNPSIGYVASGYWFGLTFGRLVLTRLIHKLIGARRGNSIAIALSILFALLTWVIPSLPIEIVTITLVGIMIGPVYPLMITYIVSEDLIPRKIAVISVTVMTAFGSSGGAMLPFLVGLISQFAGAFVVLPSFIAMFFVTLCLWLLLPNASYTKGTGKIDILRRIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU53</accession>
    <name>G3AU53_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Lysophospholipase</fullName>
            <ecNumber evidence="2 10">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_157443</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the release of fatty acids from lysophospholipids. Phospholipase B may well contribute to pathogenicity by abetting the fungus in damaging and traversing host cell membranes, processes which likely increase the rapidity of disseminated infection.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the lysophospholipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="2 10"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377400.1">
        <property type="nucleotide sequence ID" value="XM_007377338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU53"/>
    <dbReference type="STRING" id="619300.G3AU53"/>
    <dbReference type="GeneID" id="18871080"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157443"/>
    <dbReference type="eggNOG" id="KOG1325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014602_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU53"/>
    <dbReference type="OMA" id="QLWSHIP"/>
    <dbReference type="OrthoDB" id="4084751at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000010">
        <property type="entry name" value="Lysophospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF33">
        <property type="entry name" value="LYSOPHOSPHOLIPASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9 10" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6 9 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012519756" description="Lysophospholipase" evidence="11">
        <location>
            <begin position="16"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="PLA2c" evidence="12">
        <location>
            <begin position="28"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW30429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="601" mass="66353" checksum="D67BBC3A84EFFEDB" modified="2011-11-16" version="1">MHVLAILLLIQLAIAISPTKNYTPGKVKCPGGKLTREANGICASEVEYVNNRKDIAKTNLRQFLKSVSMRDFDVDGFLDSGAPLPVIGMAFSGGGYRAMLCGAGQISAYDSRVKSKDKTLAGLLQSTTYLSGLSGGNWLVGSLISNNFISIDEVLAHKKLWDIDNALLDYYGDNFIANKIMWAAISMQVWSKDLAGFETSMTDIWGRALSYPLLAATEDQGDAFLLSDVTSLPAFKSYDIPFPILVADGRAPNSTIINLNSTVFEITPYEVGSFDPSLNTFVQTKYLGTQLDDGVPKKDCINGYDNAGFLMGTSSSLFNQFILQLDKAGLPPFINDLIKKYILDPLSKSNEDVANYNPNPFYKSTNPETTISGSKILHLVDGGEDGQNVPLLPLLNRKVDIIFAHDNSNDHKGFPDGTSLIATYERQFVQQGKSMAFPYVPGQSSFRNLNLTSKPTFFGCDAKNLSTLTDNIYDVPLVVYIGNRPFSYWSNTSTFKMEYKENERRGMIKNGYEVATRKNGTLDSDWAACVGCAIIRRQQERTGQEQSEQCKQCFKRYCWDGTIYKGADIGENYDDEGLTLTGKYYNGNNIPGIKDGGTELY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU70</accession>
    <name>G3AU70_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Aminomethyltransferase</fullName>
            <ecNumber evidence="2 8">2.1.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 8">Glycine cleavage system T protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_63281</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">The glycine cleavage system catalyzes the degradation of glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[protein] + (6S)-5,6,7,8-tetrahydrofolate = N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16945"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10492"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="2.1.2.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the GcvT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.10" evidence="2 8"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377417.1">
        <property type="nucleotide sequence ID" value="XM_007377355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU70"/>
    <dbReference type="FunCoup" id="G3AU70">
        <property type="interactions" value="1252"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU70"/>
    <dbReference type="GeneID" id="18874675"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63281"/>
    <dbReference type="eggNOG" id="KOG2770">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_10_0_1"/>
    <dbReference type="InParanoid" id="G3AU70"/>
    <dbReference type="OMA" id="MPVQYPA"/>
    <dbReference type="OrthoDB" id="10263536at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004047">
        <property type="term" value="F:aminomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006546">
        <property type="term" value="P:glycine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1400:FF:000001">
        <property type="entry name" value="Aminomethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.110">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1400">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1250.10">
        <property type="entry name" value="Aminomethyltransferase fragment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006223">
        <property type="entry name" value="GcvT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028896">
        <property type="entry name" value="GcvT/YgfZ/DmdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029043">
        <property type="entry name" value="GcvT/YgfZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013977">
        <property type="entry name" value="GcvT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006222">
        <property type="entry name" value="GCVT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00528">
        <property type="entry name" value="gcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001567">
        <property type="entry name" value="PRK00389.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757:SF2">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01571">
        <property type="entry name" value="GCV_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08669">
        <property type="entry name" value="GCV_T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006487">
        <property type="entry name" value="GcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101790">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="GCVT N-terminal" evidence="9">
        <location>
            <begin position="21"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Aminomethyltransferase C-terminal" evidence="10">
        <location>
            <begin position="314"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006487-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="395" mass="43338" checksum="C249FF22BEC85B6B" modified="2011-11-16" version="1">MLRTSAKRFYSAPANLLKTPLHEAHVALGGKMVPYAGFEMPVLYNSQSHIDSHNWVRSKVGLFDVSHMLQHKITGKDAKSLLQKITPIDLNSLPMNESSLSVLLNENGGIIDDCIITKHGEDSFYMVTNAGCRAKDVEFIKKEAEAFGSSVNHNTFEGTLLAIQGPKAQELLQKFTNEDLSKIYFGQTRFVKLSPIGATVHLARSGYTGEDGFELSIPSSNAAETKDSLNFFQSLIAEYPDLVKPIGLAARDSLRLEAGMCLYGYELTEQITPVEASLSWLIPKARRDANAGFNGASKILSQINDKSLVTHRRIGVVSKGPSPREHFKIFSNDGSQEIGYITSGSPSPTLGGNIAQAYIDKKFKIGSDIKIEIRGKLRDGKITKLPFVASNLYKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU75</accession>
    <name>G3AU75_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_143158</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377422.1">
        <property type="nucleotide sequence ID" value="XM_007377360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU75"/>
    <dbReference type="GeneID" id="18870523"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143158"/>
    <dbReference type="eggNOG" id="ENOG502RXZ0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063930_2_0_1"/>
    <dbReference type="InParanoid" id="G3AU75"/>
    <dbReference type="OMA" id="VMNVMIR"/>
    <dbReference type="OrthoDB" id="410198at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052523">
        <property type="entry name" value="Trichothecene_AcTrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791">
        <property type="entry name" value="GNAT FAMILY ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="265" mass="29923" checksum="B21B542258DBDD99" modified="2011-11-16" version="1">MLKTTTSDTISATPRATIKISNTSTIRHLTNADVRKAALCLLEAFKEDSLAKLLVCHLPENKRKYAELCLYEAYLRQHIKHGIVLGQGETNEGFETVSIWACPESVHQGLDSFGTLMESGYDKVWQVFGEEGRQKVFYGMLPLLHDSCERIINNDSRFKNKGVYTLVYVGSRDCARGKGNLRKMFEYMFTKYIDADDSLAYLESSSPNNIPIYERFGFKVVEDIVLGEKSDGAVEGEHFAVMNVMIRGVKGHDWTQDENISKGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUE7</accession>
    <name>G3AUE7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Maintenance of ploidy protein MOB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_63359</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377494.1">
        <property type="nucleotide sequence ID" value="XM_007377432.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUE7"/>
    <dbReference type="FunCoup" id="G3AUE7">
        <property type="interactions" value="552"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUE7"/>
    <dbReference type="GeneID" id="18874716"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63359"/>
    <dbReference type="eggNOG" id="KOG0440">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038321_4_2_1"/>
    <dbReference type="InParanoid" id="G3AUE7"/>
    <dbReference type="OMA" id="VDNEQMF"/>
    <dbReference type="OrthoDB" id="8170117at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035974">
        <property type="term" value="C:meiotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071958">
        <property type="term" value="C:new mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071957">
        <property type="term" value="C:old mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902554">
        <property type="term" value="C:serine/threonine protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034973">
        <property type="term" value="C:Sid2-Mob1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903473">
        <property type="term" value="P:positive regulation of mitotic actomyosin contractile ring contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031031">
        <property type="term" value="P:positive regulation of septation initiation signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007096">
        <property type="term" value="P:regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.30">
        <property type="entry name" value="MOB kinase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005301">
        <property type="entry name" value="MOB_kinase_act_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036703">
        <property type="entry name" value="MOB_kinase_act_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03637">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101152">
        <property type="entry name" value="Mob1/phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605301-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="276" mass="31696" checksum="13A9B46EC3CBEAF9" modified="2011-11-16" version="1">MSFLQNFNAHSLKSTRGFKLKQSSPISSPQPIGNSVDSSYPQEYSQYQTINTNLKLNNDDTNTMSGTAGVSTHKDIRNYAEQTLGSDNALIQAVKLPRDEDLNEWLAIHVVDFYNQINMLYGAITEFCSPATCPRMIATEEYEYLWQETPMDQHRGDSNAPPSSPRRPVSLPACEYIENLMNWVQGFFDNDNIFPSKIGAPFPQQFPSLVKTIFKRLFRIYAHIYCHHFHEISELGLQSHLNTSLKHYVLFANEFQLISRKDYGPLEDLVDTMLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUR1</accession>
    <name>G3AUR1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_157880</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. SPB1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377588.1">
        <property type="nucleotide sequence ID" value="XM_007377526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUR1"/>
    <dbReference type="FunCoup" id="G3AUR1">
        <property type="interactions" value="1156"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUR1"/>
    <dbReference type="GeneID" id="18871103"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157880"/>
    <dbReference type="eggNOG" id="KOG1098">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009422_8_1_1"/>
    <dbReference type="InParanoid" id="G3AUR1"/>
    <dbReference type="OMA" id="QRKDKYY"/>
    <dbReference type="OrthoDB" id="1287559at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000004">
        <property type="entry name" value="AdoMet-dependent rRNA methyltransferase SPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03163">
        <property type="entry name" value="RNA_methyltr_E_SPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012920">
        <property type="entry name" value="rRNA_MeTfrase_SPB1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024576">
        <property type="entry name" value="rRNA_MeTfrase_Spb1_DUF3381"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028589">
        <property type="entry name" value="SPB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF13">
        <property type="entry name" value="PRE-RRNA 2'-O-RIBOSE RNA METHYLTRANSFERASE FTSJ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11861">
        <property type="entry name" value="DUF3381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07780">
        <property type="entry name" value="Spb1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3 8" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="10">
        <location>
            <begin position="25"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3381" evidence="12">
        <location>
            <begin position="236"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA methyltransferase SPB1-like C-terminal" evidence="11">
        <location>
            <begin position="589"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="483"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="554"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="348"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="443"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="705"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="488"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="563"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="583"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF11861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW30617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="807" mass="92950" checksum="00988FE563A1058A" modified="2011-11-16" version="1">MGKTQKKNSKGRLDRYYYLAKEKGYRARSSFKIIQINEKFGHFLEKSKVVVDLCCAPGSWCQVASQLCPVNSLIVGVDIVPIKPMPNVITFQSDITTEDCRSKLRGYLKTFKVDTVMHDGAPNVGLGWVQDAFTQSQLTLQALRLAVEHLAPNGNFITKIFRSRDYNNLLWVFQQLFEKVEATKPPASRNVSAEIFVVCKGFKAPKKLDPRLLDPKEVFEEIDRVETNNEAKVFNPEKKTRHRTGYEEGDYTLYHTMPILEFIKDEDPINQLGRLNKLEVPTDDHEWKVVSKLRSCTPELLECLKDLKTLGRKEFKLILKFRKQARELLQLDLPEEKPEIEVEELTEEQKIDKELQELMDKQKQKQKRLKKQANELKQKEIVRNQMNMITDMNIGIEASNIGAESLFNLKTAEKTGQLDKLAKGKKRMVFTEQELAKDNEIHIDEEAEDINSADELENQLEDMYQNYQARKAEKDVNYRAKKLRENDEPWDGIDDEPEEVESENDYEMEEDSESDSDDDEQINKIIEARKANELSKNAKAFFSSDSIFKELGDDALLEEMTKPTETNGSTKPQPQKPVQPADDSSDSSDSENDDFEVVPNEESDYDDSDSDTETKTKTHQKNLDLVTVEAMTLAHQVALGQMNKNDLVNEGFHRYSFRDKDNLPDWFLDDENKHSKLIKPITKEAAMAIKEKQKQLNARPIKKVLEAQGRKKMRALRRLEKLKKKSDLINEDSGKSERDKAEEIQKLMKKLTKKQKQKPKISVVVARGANKGLSGRPKGVKGKYKMVDGVMKNEQRALRRIAKKHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV67</accession>
    <name>G3AV67_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_63490</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377636.1">
        <property type="nucleotide sequence ID" value="XM_007377574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV67"/>
    <dbReference type="GeneID" id="18874777"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63490"/>
    <dbReference type="HOGENOM" id="CLU_3070178_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV67"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW29870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="53" mass="5851" checksum="1CD0FF18853D2101" modified="2011-11-16" version="1">MRSFANICATIAQLYRCGNFRAIVESVSYLLTYTKQLIGQAIYGRESAKSGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV73</accession>
    <name>G3AV73_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_52731</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377642.1">
        <property type="nucleotide sequence ID" value="XM_007377580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV73"/>
    <dbReference type="STRING" id="619300.G3AV73"/>
    <dbReference type="GeneID" id="18871715"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52731"/>
    <dbReference type="HOGENOM" id="CLU_460908_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV73"/>
    <dbReference type="OrthoDB" id="4022214at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442391" description="Hyphally-regulated cell wall protein N-terminal domain-containing protein" evidence="7">
        <location>
            <begin position="17"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="8">
        <location>
            <begin position="8"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="459"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="519"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW29876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="592" mass="66657" checksum="63EE05356D79547D" modified="2011-11-16" version="1">MLYFIFFCFLFSPIFGQQITTDTTVREAITSGEFLDVFSPAHYDLVDVKSVEIDGNIYNNGSITARSPSSYSTKINLSGDYFTNYGTFSADLYDGKFDLSYYSFNNYGNIFLTDGDISIATSQFHNYGHVQFYDSHYPSISTTGGNTENEGSICYRNSIDGIYSYYSGTGCIVLDSSELYINEKFAYTPQTIHLTNNSHVLFDETPLGLSFSGFGDDSTIGSFSKLYSYTYDTYLGALQLYYYNSSILVYIGKGYDESKFEKVTYAGSYTADDYNSIKYNGPCPNAPYDLCYCADKQVYTDKNKDKDKSSNCDAETVTTTVFKNAPTKTVTVTKTETTCPTNGGDVVYKKKPCDCYTYTYTYTEDYVTFTETCTTDVATFIYYHSYICTVTYVDATTTIWYKTYTTDTYTCHHTSTDYETVTTWYDTETTSWDDTTTWSESETTSWTDTDTWSDSTTWDDSTTWSDSTTWDDSTTWSDSTTWDDSTTWSDSTTWDDSTTWSDSTTWDSTSTWVDIPSLETPTTSVLEETPTDSDESIGTPIIDDPPILSFDDNKPLETPVASADPPILSFDDNEPLETPVASADPPQLSFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV98</accession>
    <name>G3AV98_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">proline--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Prolyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_73354</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Pro) + L-proline + ATP = L-prolyl-tRNA(Pro) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="2"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377667.1">
        <property type="nucleotide sequence ID" value="XM_007377605.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV98"/>
    <dbReference type="FunCoup" id="G3AV98">
        <property type="interactions" value="692"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV98"/>
    <dbReference type="GeneID" id="18875479"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_73354"/>
    <dbReference type="eggNOG" id="KOG4163">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001882_4_1_1"/>
    <dbReference type="InParanoid" id="G3AV98"/>
    <dbReference type="OMA" id="EVYWVTH"/>
    <dbReference type="OrthoDB" id="1350766at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00862">
        <property type="entry name" value="ProRS_anticodon_zinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00778">
        <property type="entry name" value="ProRS_core_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.30:FF:000001">
        <property type="entry name" value="Bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000007">
        <property type="entry name" value="Bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000005">
        <property type="entry name" value="bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.30">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.960.10">
        <property type="entry name" value="YbaK/aminoacyl-tRNA synthetase-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01571">
        <property type="entry name" value="Pro_tRNA_synth_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004499">
        <property type="entry name" value="Pro-tRNA-ligase_IIa_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016061">
        <property type="entry name" value="Pro-tRNA_ligase_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017449">
        <property type="entry name" value="Pro-tRNA_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033721">
        <property type="entry name" value="ProRS_core_arch_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036754">
        <property type="entry name" value="YbaK/aa-tRNA-synt-asso_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00408">
        <property type="entry name" value="proS_fam_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382:SF2">
        <property type="entry name" value="BIFUNCTIONAL GLUTAMATE_PROLINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382">
        <property type="entry name" value="PROLYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09180">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SMART" id="SM00946">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64586">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55826">
        <property type="entry name" value="YbaK/ProRS associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="11">
        <location>
            <begin position="212"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="627"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW29901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="684" mass="76038" checksum="4937718527239203" modified="2011-11-16" version="1">MALEQSLAALSLATVSADSSAVDTKTLVFKPKTAKTATPIPLFVFALTATQTPSSYIAKAAGVKEPRLANDELVQEFFATTTKEVSIASLSSALAGKVKLVVDAAVFKAAETDAVLKLTTESSGAALPAKTVVEFLKSTGIELIEVDFSVEPAAAAAPAAAPAKKEKKEKDSGKIEDAKLIGITYDKEKDFSSWYTEIVTKGELLEYYDVSGCYILRPNSYFVWETIQDWFNVKIKSLGVQNTYFPMFVSQRVLEKEKDHIEGFAPEVAWVTRAGSSDLEEHIAIRPTSETVMYPYYAKWIRSHRDLPLKLNQWNSVVRWEFKHPQPFLRTREFLWQEGHTAHLTKEGAQQEVLEILDFYAAVYEDLLAVPVVKGRKTENEKFAGGDYTTTVEGFISATGRGIQGGTSHHLGQNFSKMFNIAVENPEGADKPKVFAYQNSWGLSTRVIGVMIMTHSDNKGLVLPPRVAQNQVVIIPVGLTSKTSDEQRKQINEGAEEIEARLKKSGVRAIGDYRAIYNPGWKFADWELKGVPVRLEFGPKDLAQNQVTAVRRDDGSKYTIKLEELEQRIPEILDEMHNALLSKARNDFDAHRVKVDEWKDFVPTLNAKNVILSPWCGDADCEDDIKDSSAKKDNGEDEEVDEKAPSMGAKSLCIPFEQPELKPGQKCVKCDKLASTYCMFGRSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVL3</accession>
    <name>G3AVL3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_158212</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377728.1">
        <property type="nucleotide sequence ID" value="XM_007377666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVL3"/>
    <dbReference type="GeneID" id="18871118"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_158212"/>
    <dbReference type="eggNOG" id="ENOG502RQG4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016726_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVL3"/>
    <dbReference type="OMA" id="RYNGCPF"/>
    <dbReference type="OrthoDB" id="4034597at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW29962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="692" mass="78825" checksum="884E49F59B6CFA8E" modified="2011-11-16" version="1">MILQETALNSLKGVYIDPKCQSAHDVISQDTVYTFMLEMIHLNYILEKPVIDDLCKLSSVEGGKFLKDFLSIIQKNIGAHVKYEPMYPNFPQQVMNASDVELVLNAILHYWSFGAWRPVYIKEKRVELDEKNPLEKLKLISLDDLREYFITLINSKTGIPVIYDEFIREGLNLGWFEDYKAKFPEIPFRVTSCKLAVECLKQGKSIDYFVKTTTDVLRIMAVYSGQPPELDKVVKFKSMRRKERRVLIQNLERVISVEDVKRHTSIWIRAFHCLHVGEYGGKVNEIASRFRNENNVATKNTALCKAIEASDIDTAVKILKNLPSMFARYLDKLLRDSQHDQETLLEFSNIAAGVESKILLQALGHFKGNNKVEQKTVFAKSKLILIEKKENQKVLANSVAVKVVDTIRKALVEKYKLLNHFNKDSKVYITKEAEGILLPMQLNTGSNSNKRSIARGSRLLPNEADLDKNIIRFFVYWVGRDIDLSGVFLAEDLETTSYINYTNLREGYAVHSGDITSAPNGASEFIDIDITKALADGFRYVEMSVRVFSGPNFVEHEECFAGYMMRQEAQAGEIFEPSTVRTKMDLVCETRDINPFMFDLVTKELIWLDIPSHPKVFGPNNVNVNINQIKETLKASLELPLLKVNMRELLDLHVEAGSATIVDDPKDANFVVGLGDGDLDLYDFATINSKWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVM9</accession>
    <name>G3AVM9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="10">2.7.8.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_63807</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Functions in the biosynthesis of the anionic phospholipids phosphatidylglycerol and cardiolipin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>a CDP-1,2-diacyl-sn-glycerol + sn-glycerol 3-phosphate = a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12593"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60110"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the CDP-alcohol phosphatidyltransferase class-II family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.5" evidence="10"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377960.1">
        <property type="nucleotide sequence ID" value="XM_007377898.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVM9"/>
    <dbReference type="FunCoup" id="G3AVM9">
        <property type="interactions" value="416"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVM9"/>
    <dbReference type="GeneID" id="18874924"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63807"/>
    <dbReference type="eggNOG" id="KOG3964">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030471_1_1_1"/>
    <dbReference type="InParanoid" id="G3AVM9"/>
    <dbReference type="OMA" id="HKCLAQC"/>
    <dbReference type="OrthoDB" id="10250191at2759"/>
    <dbReference type="UniPathway" id="UPA00084">
        <property type="reaction ID" value="UER00503"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09135">
        <property type="entry name" value="PLDc_PGS1_euk_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09137">
        <property type="entry name" value="PLDc_PGS1_euk_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016270">
        <property type="entry name" value="PGS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586:SF1">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--SERINE O-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000850">
        <property type="entry name" value="Phospholipase_D_PSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="11">
        <location>
            <begin position="183"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="11">
        <location>
            <begin position="419"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW30194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="532" mass="60654" checksum="D0A41D7B108B600D" modified="2011-11-16" version="1">MLRSTRTTRVSMLSSIYNYFRGITPLPTTPQRTFTTNPSLPENAFHPRLSPIISQLDSLAPRFALHSDDIDILSSPDEFYNLLTQKISAAKSRIYLSSLYIGKKQTDLIDCIDQAMSKNDELKVHILTDALRGTREAPNPCSASLLVGLVEKYGKHRIDVRMYHTPHLSGITKSITPKRINEGWGLQHMKLYGFDEEIILSGANLSQDYFTNRQDRYYVFKNKSITDYYFRIHTAISSLSYQVTESKLKQGFKLTWPTSNKSCEPHMNLHRFISDSGHLLEPILKQHPITDDGSDFDTIVYPVSQFTPLFPQNQDSSTEKPSILRILTYLDSPSIKWWFTAGYFNMLPEIQHRLLNGKAEGSVITASPKANSFYKSPGVSYYLPQAYLLFTKQFLQQVKQKHSAITVYEWENGEVNTPEGWSYHAKGLWVSIPNETKPSITIIGSSNYTKRAYSSDLESNAVIITKSDKLKSKMKQEIDNLMKHTQELSLDDFQPRAVPGTNEQEPPRYVIDEDRKITYGVHLAVKLLGGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVU9</accession>
    <name>G3AVU9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein RER1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_52840</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the retrieval of endoplasmic reticulum membrane proteins from the early Golgi compartment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the RER1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377760.1">
        <property type="nucleotide sequence ID" value="XM_007377698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVU9"/>
    <dbReference type="FunCoup" id="G3AVU9">
        <property type="interactions" value="849"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVU9"/>
    <dbReference type="GeneID" id="18871724"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52840"/>
    <dbReference type="eggNOG" id="KOG1688">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074889_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVU9"/>
    <dbReference type="OMA" id="GWYVVCY"/>
    <dbReference type="OrthoDB" id="448250at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030137">
        <property type="term" value="C:COPI-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004932">
        <property type="entry name" value="Rer1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10743">
        <property type="entry name" value="PROTEIN RER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10743:SF0">
        <property type="entry name" value="PROTEIN RER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03248">
        <property type="entry name" value="Rer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016013">
        <property type="entry name" value="AtER_Rer1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR016013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW29994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="191" mass="23045" checksum="D7B6FC2DEC4AC24D" modified="2011-11-16" version="1">MAIELPFEIPKDNFIYQNYSKIVVTYHSFIDESVPHTRNRWIGFSVLIALFLLRIFMAQGWYIICYALGIYLLNLFLAFLTPKFDPSLEQELKNESIEEGVQDEEPDKDSDEFRPFIRRLPEFKFWYNATRATIVALFLSLFEIFDIPVFWPILLMYFIILFTLTMRKQIQHMIKYKYLPFDFGKARYRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVX7</accession>
    <name>G3AVX7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_63854</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007378004.1">
        <property type="nucleotide sequence ID" value="XM_007377942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVX7"/>
    <dbReference type="FunCoup" id="G3AVX7">
        <property type="interactions" value="428"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVX7"/>
    <dbReference type="GeneID" id="18874945"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63854"/>
    <dbReference type="eggNOG" id="KOG3771">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072096_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVX7"/>
    <dbReference type="OMA" id="TRFCAYF"/>
    <dbReference type="OrthoDB" id="446293at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990528">
        <property type="term" value="C:Rvs161p-Rvs167p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000747">
        <property type="term" value="P:conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060988">
        <property type="term" value="P:lipid tube assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030100">
        <property type="term" value="P:regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006970">
        <property type="term" value="P:response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000014">
        <property type="entry name" value="Protein hob3, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046982">
        <property type="entry name" value="BIN3/RVS161-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174">
        <property type="entry name" value="BRIDGING INTEGRATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174:SF3">
        <property type="entry name" value="BRIDGING INTEGRATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAR" evidence="4">
        <location>
            <begin position="15"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="265" mass="30216" checksum="A577691E33B5F4DF" modified="2011-11-16" version="1">MSWGGFKKAVTRAGQSITVKDVDKTMDKDFDIEERRYKVLKTAGTNLQKAVKGYLDSLRAVTASQVTIAEIVSNLYEESKQGPGQNNNIGTYYMQCVREFDEETVKQLDGPFRETVLDPITKFANYFLEIDEAIKKRAHKKIDYDQCKAKVRRLIDKPSKDAGKLPRVQKELQVAKEIYEDLNEQLKSELPQLIALRVPYLDPSFESVVKIQLRFCTEGYSRLAQIQQYLDPASRDEYANGLLDGKIDDMIVQMNGLNITSLGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE71</accession>
    <name>G3AE71_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_64707</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373017.1">
        <property type="nucleotide sequence ID" value="XM_007372955.1"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AE71"/>
    <dbReference type="GeneID" id="18874993"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64707"/>
    <dbReference type="eggNOG" id="KOG0585">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_272804_0_0_1"/>
    <dbReference type="InParanoid" id="G3AE71"/>
    <dbReference type="OMA" id="QFNNHYK"/>
    <dbReference type="OrthoDB" id="68483at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001558">
        <property type="term" value="P:regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14008">
        <property type="entry name" value="STKc_LKB1_CaMKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001578">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000206">
        <property type="entry name" value="Serine/threonine-protein kinase Ssp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF152">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE TOS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="212"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="709"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="768"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="815"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1055"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1155"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1240"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="164"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="733"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="770"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="827"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1055"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1164"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1252"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW35605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1299" mass="145441" checksum="0EEACF60645B470A" modified="2011-11-16" version="1">MSSSSPLMQSSTAINCSHNNSNMSTNTTTTTATTSTSTTTSRKQSFILNKNGTTAAVASPTIGISTFDPISGHTDYTHDNNIISSQCSPLGSPRQLPQDDFRSNIFNNVNGIFSSNDSRSNSITSLKEHLHNHHQNSIHKRASSDVEFVPPQHKPELLSPRSKFATTTPNTPQPNIFNSYPYKSYHSSPVKETNRVLLEYDPINRRKVLNTYEILREIGRGEHGKVKLAKDLINNDYVAIKIVNRKSKKERPSLRLRKESRPSHHLSDYELKIKREIAIMKKCRHKHIVSLREVLDDVNSYKIYLVLEYMEKGEIKWKRSPTDKPTEENESDIPCCGSLIQQPYFGKGKKRYSLDENDLLSNEFSPNLTFKQGRRIFRDVLLGLEYLHLQGIVHRDIKPANLLVSAENIVKISDFGVSFASSLSETEEGHLVNELDLAKTAGTPAFFAPELCRFGDITSPASSASSSVSLDQFKTPKIDYKIDIWALGVTLYCILFGRVPYNADSEYELFQVIVNNPLEFPETKESFNSPGPVTDEEFNLAKDLLSKLLDKDNTTRIDIIEIKEHPFTLMDLENDLEGLSELFTLNQTLQEENPLNFNLEEHDIVSQQEIENAVIGIGTRIKRNLVKAIRAGGWKDQEIRNKFAALQLEHSKSGSSEESSGCSNFNSQTKLSNLQNIDNHNHSMILSEGLQVTATPPPIQLQSGWTNSVSRVASHDQHPPHVPSALAHQLPVSSSSSTTSHSFSSPFSFAGIREGERVTGRSLLQEMIESHSNSSSRRGSSSGGVLSEAPQIETKRNVGGDLYLKNQSVVETFKGIQQQDDKRRRSSLFSLHSQNSSNNTKNNSISSTHEEYSRNHPYQQQQHHHHHHPNTNIAAPIPVPVANLKTNMTGSTANTSNYMNNNGGLHSEMSSRPSLKVGPITLPSVFNTKDVATIIDIDDNPESSVMSLPLSESYASLDSINDEYLSMKYEEFTNSRNLNCQSEALPVIVENGKKLRKSSFSETYLKELDQDLDIKEINQKFKTFNLGDSMKSNQDLENIDKDNTIAPRTKIRYSWSSSSTSSSSSQDDGASGSDEESDEENLTLAFSSKVTPLRPGLLSFNNRAKSHESNLPRLVHHPSRDLPAVVFHGALPEFEDVPMDLMDASVTAGSLMKPSGRLIPPSEPLNQNRSLGSSTGSQATLTAEGIQADSSVETTLIPEKSESMTKIKLLQSQLSKKNMQPRRGLRENIFHNQFNNHYKKDPIYSPFPSRSHHLDNDKKSMNKEEIRPNSYRSNSVTLELLQHQRHLFEQQQIEQSQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE78</accession>
    <name>G3AE78_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CP-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_132696</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in the mitochondrial lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373024.1">
        <property type="nucleotide sequence ID" value="XM_007372962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE78"/>
    <dbReference type="FunCoup" id="G3AE78">
        <property type="interactions" value="1071"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AE78"/>
    <dbReference type="GeneID" id="18869684"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132696"/>
    <dbReference type="eggNOG" id="KOG2484">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011106_5_5_1"/>
    <dbReference type="InParanoid" id="G3AE78"/>
    <dbReference type="OMA" id="FKLDGLW"/>
    <dbReference type="OrthoDB" id="10266128at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04178">
        <property type="entry name" value="Nucleostemin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1580.10:FF:000006">
        <property type="entry name" value="Nuclear GTP-binding protein NUG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000844">
        <property type="entry name" value="Nuclear GTP-binding protein NUG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014813">
        <property type="entry name" value="Gnl3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF30">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN-LIKE 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08701">
        <property type="entry name" value="GN3L_Grn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CP-type G" evidence="10">
        <location>
            <begin position="167"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="67"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="466"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="96"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="127"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW35612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="514" mass="57343" checksum="82DDD0A426BC5AC8" modified="2011-11-16" version="1">MRVKKPTSKRVSTRMREGIKKKAAAQQRKNRKMAKKDITWKSRHKKDPGIPASFPYKDKIITELEEGRRLERERKEQLKLQRQQEKEAALARGEIVDDDMEQDDDESEEGGLAALLESAQQAAKEYDGDDFEGEDDNMVDSDEDVEYDIEFDDDEDTGDLDKSRKAYDKIFKTVVEAADVILYVLDARDPESTRSRKVEQAVLQNPGKRLILVLNKVDLIPTNVLNQWLDFLKSSFPTVPIKAAPGATNSTSFNKNLTNSMTSDSLLKALKSYASKSNLKRSIIVGVIGYPNVGKSSIINALTNRHGGSAKACPVGNQAGVTTSMREVKIDNKLKILDSPGIVFPDEIINTKKLSKTQQMAKLALLSAIPPKQIIDPIPSIQMLLKKFSNNPEMADGLKNYYQLPPLPSGDFEEFTKHFLIHIARTKGRLGRGGVPNMESAAMSVLNDWRDGRIIGWTLPKASKTTAEEVDLDAPKSSLRGEKEPPKVEQTTIVQTWAKEFDLDGLLGDNFGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEV5</accession>
    <name>G3AEV5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">26S proteasome regulatory subunit 6A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_58995</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373197.1">
        <property type="nucleotide sequence ID" value="XM_007373135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEV5"/>
    <dbReference type="FunCoup" id="G3AEV5">
        <property type="interactions" value="1000"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEV5"/>
    <dbReference type="GeneID" id="18872712"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58995"/>
    <dbReference type="eggNOG" id="KOG0652">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_2_4_1"/>
    <dbReference type="InParanoid" id="G3AEV5"/>
    <dbReference type="OMA" id="NKISHEH"/>
    <dbReference type="OrthoDB" id="9443236at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045899">
        <property type="term" value="P:positive regulation of RNA polymerase II transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000076">
        <property type="entry name" value="26S protease regulatory subunit 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000037">
        <property type="entry name" value="26S protease regulatory subunit 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 8" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="207"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00069320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="404" mass="44786" checksum="72558BB447C48CC5" modified="2011-11-16" version="1" fragment="single">MVTLEELENNKEDDLFDQEILNSSTSDIINRTKLLDNDIKVMRSESQRLTHEKTMMLERIKDNQEKINNNKQLPYLVGNVVELLDLDAEKEASEQGANIDIDAARSGKSAVIKTSTRQTIFLPMIGLVDPTTLKPNDLIGVNKDSYLILDTLPSEYDSRVKAMEVDEKPTEDYSDIGGLDKQIEELIEAVVLPMKQAEKFQTLGIKPPKGALMYGPPGTGKTLLARACAAQSGATFLKLAAPQLVQMFIGDGAKLVRDAFALAKEKAPTIIFIDELDAIGTKRFDSDKSGDREVQRTMLELLNQLDGFGSDDRVKVLAATNRVDTLDPALLRSGRLDRKIEFPLPSEEARESVLKIHARKLNCDNNSVNWRELARSTDEFNGAQLKAVTVEAGMIALRNGKSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEW1</accession>
    <name>G3AEW1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_131497</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373203.1">
        <property type="nucleotide sequence ID" value="XM_007373141.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AEW1">
        <property type="interactions" value="1324"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEW1"/>
    <dbReference type="GeneID" id="18869581"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131497"/>
    <dbReference type="eggNOG" id="KOG0951">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000335_1_0_1"/>
    <dbReference type="InParanoid" id="G3AEW1"/>
    <dbReference type="OMA" id="MNPKEFN"/>
    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000388">
        <property type="term" value="P:spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000133">
        <property type="entry name" value="Pre-mRNA splicing helicase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000102">
        <property type="entry name" value="RNA helicase, activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000024">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000001">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000062">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000013">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein kDa helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041094">
        <property type="entry name" value="Brr2_helicase_PWI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048863">
        <property type="entry name" value="BRR2_plug"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21188">
        <property type="entry name" value="BRR2_plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18149">
        <property type="entry name" value="Helicase_PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039073">
        <property type="entry name" value="BRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="401"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="592"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="1242"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2024" mass="230441" checksum="FF82200942444A38" modified="2011-11-16" version="1">MANKVIRQPRKGTSEQEVPASSMAGRINAKDMGSNYKPEHKTVTKTTKQEENSSLPNYQLFEVFMTKIRTYLPDSSHEVIRSATELAVEYLSKPDLSVVKKREELEELLSAKVDDVDLNVLINLTDKMNELNSVKQDDNENEYIAVDFDSSDEEQGQADVEVEVEEDEEQPEGEQEVIELPEVSKDPEVVNIAWLEQEVLKASKTHELSTSAELLLSDTIRLLSDVELTVNELDNSLNEVFEYECSDLIVKCIENRWRIVFTKKLSEATSEETRQEIYQEMEALHLEQLASELEDKKRSAFDDEAEVTSVKRVKQGKRIPQRISLDKIQFSTSVEGTKVILPKGSFQQNKKSYDIITVPPVEQAPQTEDLLPINSLPDWARDAFPSNETTTFNRIQSKIFPQAFETDNNLLICAPTGAGKTNVAMLTILRTISNFRTEAGRILLKQFKIIYIAPLKALVQEQMREFQRRLTSYGLVVNELTGDSTLSKRQILETQIIVTTPEKWDIITRKDPSYISLTRLIIIDEIHLLHDERGPALENIVGRTLRKSETTGNQVRLVGLSATLPNYADVAKFIQVPEEGLFYFDASFRPCPLQQEFVGIKERSAIKKLNAMNEACFDRTLNSLERGHQLIIFVHSRKETYTTAKYLMDKMASSEVNMVDTLGVKEILKQEGESMSNKHLKEIIPNGFGIHHAGLSRNDRNVVEDLFASGHLRVLVSTATLAWGVNLPAHTVIIKGTETYSPEIGTWVQLSPQDILQMLGRAGRPRYDKNGEGIIITSQDEIQYYLAILNQQLPIESKLISKLVDSVNSEVVSGSITTLEEGIEWLSYTYFFVRMLHSPALYGIEADYDFKGDPTLHNRRADLIYTALAILHENKLVMFESETGLVKSTELGKISSYYYINYETINLYGKMLKPWYNEIDILRVFSNSGEFKYVPVRQEERLEVSKLMEKCPIPIKEQPHEAVAKINILLQTYISRLALEGYALISDMIYITQSAGRLLRAIYEISLLKKWSGLSKIVLDLCKMVDKRIWLNNSPLRQFGSLVPDQIIRATEMSHLPWNRYFQLTVEELAEAINLKGNAKVASEFIQAFPRISIQYFVQPISSKFLRVQIEAVPEWSWMSVHGSQEMFMVFLEDCNGNQLLHHEEFVVKQQNINKVHVVEFIVPVSEPLIPNFVVSFISNKWVNCNWKVPIMLTNVEVPSLSSYYLDNSDVQLVSTKDLKKQEFIDLFPFTFFNKVQSATFDVVYHTNENVFIGSSKGDGKTVCAELAILNHWRQNKGRVVYINPSTEIIDDNLKKWSNSFDVFEKSVNKLTGTLRQDIAILNESHLVLATPEQFANLSKRWKTKKSFKSIDLLILDDIHFIGSLPTYEILVSRIRMLTSQWDNMLRIVALASPVANCRDLCDWIGVGKSNVFNFPPQSRQNSISEIKLSPEGSVVPIVKIYKELKRLNVGLRKSVIFVPTRVKALELARQLLDYMAGSHDWRRVDLLKLEKYIEKVEDNTLRELLGRGIAVYFENMSRVDKLIVEKLFESNSIGILIATKNTCKYAPHGHNLLVVGTQTYDGYEHRNVDCNSNEIFEMVGCCQDDLANEGKVHIYTENKTIEFYGSLLNDGLIVESLLLSSISEFFMGAISNGVVRSKQDCIDLLTFSYFYRRLVKNPGFYELKNATSLGVSEYLSELIENAIEELEKNEFVEIEQDEEEETILPLNKTIIASHYSISYETMKLFGSLTSKSKLKDILLIITSASEFESIPVREEDGNVLSRLGQKVPIKLNGEHDIESPLYKSFILLQSHISRIEVPAELAQDRNTVVTKVLDILNACIDCLSGEGHLNALLAMDLSQMIIQAVWSTGAGSALRQIPYFDDAILARCEKHNVETVYDIMSLEDEERDEVLQLEGDRLNSVANFVNQYPNIEISYEMDTATPCVANEPKEITIKIERDEEMESLQVVCDKYPSAREEGWWLVIGDAQLRQLYAIKRVSLGLESQNFTLEFTVPTKGKANLTVWSICDSYIDADKEINFDIEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF35</accession>
    <name>G3AF35_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methylated-DNA--protein-cysteine methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.63</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">6-O-methylguanine-DNA methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">DNA repair MTase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">O-6-methylguanine-DNA-alkyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_69943</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MGMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.63" evidence="2"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373222.1">
        <property type="nucleotide sequence ID" value="XM_007373160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF35"/>
    <dbReference type="FunCoup" id="G3AF35">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF35"/>
    <dbReference type="GeneID" id="18875298"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_69943"/>
    <dbReference type="eggNOG" id="KOG4062">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_52_2_1"/>
    <dbReference type="InParanoid" id="G3AF35"/>
    <dbReference type="OMA" id="YTFIETE"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10815">
        <property type="entry name" value="METHYLATED-DNA--PROTEIN-CYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10815:SF13">
        <property type="entry name" value="METHYLATED-DNA--PROTEIN-CYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="Methylated DNA-protein cysteine methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding" evidence="8">
        <location>
            <begin position="99"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="186" mass="21146" checksum="125FF43D64DF6B70" modified="2011-11-16" version="1">MAYLYYTVIQTSTPYKALLVLDDHGLLCYASLGKHPNVLREELVKDFKSLKEYNLKSLSTIPSNEQIQKTIDGFRKGMDDPRTLNNLNIRTRIIFGTALQKKVWDKLLQIPTGTTMQYKEIAESLNIPNSSRVVGNCCGANKISLVIPCHRALAKNGKITGYRWGLDIKQYLLKQELGDNYNKIVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF51</accession>
    <name>G3AF51_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">NAD(P)H-hydrate epimerase</fullName>
            <ecNumber evidence="3 10">5.1.99.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">NAD(P)HX epimerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_133248</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-NADHX = (6S)-NADHX</text>
            <dbReference type="Rhea" id="RHEA:32215"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:64075"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(6R)-NADPHX = (6S)-NADPHX</text>
            <dbReference type="Rhea" id="RHEA:32227"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:64077"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="10">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.1.99.6" evidence="3 10"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372252.1">
        <property type="nucleotide sequence ID" value="XM_007372190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF51"/>
    <dbReference type="FunCoup" id="G3AF51">
        <property type="interactions" value="269"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF51"/>
    <dbReference type="GeneID" id="18869730"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133248"/>
    <dbReference type="eggNOG" id="KOG2585">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024853_3_0_1"/>
    <dbReference type="InParanoid" id="G3AF51"/>
    <dbReference type="OMA" id="RHLFHYG"/>
    <dbReference type="OrthoDB" id="10064708at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052856">
        <property type="term" value="F:NAD(P)HX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="entry name" value="YjeF N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01966">
        <property type="entry name" value="NADHX_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032976">
        <property type="entry name" value="YJEFN_prot_NAXE-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00197">
        <property type="entry name" value="yjeF_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13232">
        <property type="entry name" value="NAD(P)H-HYDRATE EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13232:SF10">
        <property type="entry name" value="NAD(P)H-HYDRATE EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="YjeF N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 10" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF N-terminal" evidence="11">
        <location>
            <begin position="14"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="66"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="139"/>
            <end position="145"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW34840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="247" mass="27025" checksum="0C31376FDB1E7890" modified="2011-11-16" version="1">MIKAGFKTLSAKSAAQLDGELMSTGAFSIDQLMELAGLAVAKAIYKQYPPSTGTPNKVLVLVGPGNNGGDGLVAARHLKTWGSYDPVLYYPKKTSNQLYSNLVKQLQDLQVGELQTLEEVKALLNNKGEVKLIIDSLFGFSFKPPIREPFKDLISFLSVNHDKIAPVVAVDIPSGWDVDQGPTDIDINASMLISLTAPKPCAARFVESGKDKVHYLGGRFINKEIAKKYDIEDIIDKYKNDDLIVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFA9</accession>
    <name>G3AFA9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SPS-sensor component PTR3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_64112</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372310.1">
        <property type="nucleotide sequence ID" value="XM_007372248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFA9"/>
    <dbReference type="FunCoup" id="G3AFA9">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFA9"/>
    <dbReference type="GeneID" id="18874957"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64112"/>
    <dbReference type="eggNOG" id="ENOG502QUFR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028479_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFA9"/>
    <dbReference type="OMA" id="TNFPIRC"/>
    <dbReference type="OrthoDB" id="5324744at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="727" mass="80877" checksum="065F191AF962E304" modified="2011-11-16" version="1">MDKASLTSLENLLKFPGSNHSDPVADASVLTCGCLVSESLFLQIQQGTCPNCKTTNVSILSEIKPLRELHRLITHFSTHTNTYAQSSISRRRRSSSKKSFKEEGTQQYGESMDLLSLFYKFAKEEEYTDGAKPTHATAPQIEASASVQPIEIKQPPRISTDIDIGSLQLQKTPSASPRNNNQLWGFETSTAASITNHSPELKFEKNLLKSIGEQKEYNFSKCFPFHRKLSTFPMLKFNFSSMVKSGSSIKKAISTSINSYHDYASGKEITRFVLINSKRWELYEYAVSISDIEKVQIKPQLILCGKSTGEFAETVNGLNQAIPRPSIKESIVRNEFGAKGDNTNDVEDMKKRLLQWDQIECYLTKNYLVITGTKGVLRVFNVNRTSPYLFGQPIYSYLTNFPIRCVAVSPNDALIACGITAKERLSGKEQPFVILHTLTFSETGQLGSVNPITITIPYRDPIKLISFNASSTHLVCGTSWESRYLVIKLKSDKEDNYRKPRLIWSDFTYKSARRSKAGMEDGKEFDDKLDADNELMMSNEGMTDLQFGNIFSNAVVVTSCSLKSRPPMIIHLEGAMIDSSKSHNVVDTYSIQNSVTSADEEDYSSIKSAEVIMKIPEIGSLIHRFAISPTGDGIVFLAKDGNVYLLSGPNFKNRLSTTSIANSKKIVVLLGEVANAERFSEAASIKFSPDGGKVFAVDRKGVFTVFDFTKGVPGHDPDVIKCKIINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFC3</accession>
    <name>G3AFC3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PDZ GRASP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_132416</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372324.1">
        <property type="nucleotide sequence ID" value="XM_007372262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFC3"/>
    <dbReference type="FunCoup" id="G3AFC3">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFC3"/>
    <dbReference type="GeneID" id="18869657"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132416"/>
    <dbReference type="eggNOG" id="KOG3834">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_657398_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFC3"/>
    <dbReference type="OMA" id="SSTQHIW"/>
    <dbReference type="OrthoDB" id="3318at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007583">
        <property type="entry name" value="GRASP55_65"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024958">
        <property type="entry name" value="GRASP_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12893">
        <property type="entry name" value="GOLGI REASSEMBLY STACKING PROTEIN GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12893:SF0">
        <property type="entry name" value="GRASP65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04495">
        <property type="entry name" value="GRASP55_65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51865">
        <property type="entry name" value="PDZ_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ GRASP-type" evidence="6">
        <location>
            <begin position="185"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="351"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="407"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="440" mass="48662" checksum="7B99732E41573A66" modified="2011-11-16" version="1">MFSFAKKLVDRLEGNVPQEQPIQNDAYFKDALEINNKGYGLRVLKVNPHSIAHSKGFESWFDYIIKINNHELPMTYPSLSTYTYSISDDGTLNYGSGATSEQAGSINFELLLQEISTLVQHDQELIVDVWSAKGGVVRQIVLPLQPPPTATPTTTETGSVQQLFENKFKQFGLTLQSAHLNSATFVWRILTTHQGSPAFMAQLVPRSDYIIGCDSAYPDDEYGKGLIIGGGEALLSKTVSSYYNYHYSISKEDNIPITFYVYNHEYDILRPVTVNLCRSWGSGHGKGLLGCDVGYGYLHRIPEVIGKFENNTITENPIFESNKEFTYDLNQKPDSQPSVFVPPSVPLSEVAANNTVSPKPPNVPPITTTLPPLASTPRSPTRLATARKKKHALSADLGGLSDYMNEELQKSKIKDDEFKTHSSESVTAPPPPPHSSTVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFF1</accession>
    <name>G3AFF1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58067</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372352.1">
        <property type="nucleotide sequence ID" value="XM_007372290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFF1"/>
    <dbReference type="GeneID" id="18872305"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58067"/>
    <dbReference type="HOGENOM" id="CLU_2869062_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFF1"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="64" mass="7495" checksum="9083307BCC790986" modified="2011-11-16" version="1">MRAKGKPRRRLSSKSISETFQFELEQEVVITQHIAMATTFSNMSPNYNHQQGTIQRFVFTYNVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFF9</accession>
    <name>G3AFF9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_48011</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NGG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372360.1">
        <property type="nucleotide sequence ID" value="XM_007372298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFF9"/>
    <dbReference type="FunCoup" id="G3AFF9">
        <property type="interactions" value="401"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFF9"/>
    <dbReference type="GeneID" id="18871166"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48011"/>
    <dbReference type="eggNOG" id="KOG4191">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016102_1_0_1"/>
    <dbReference type="InParanoid" id="G3AFF9"/>
    <dbReference type="OMA" id="WQEYSSI"/>
    <dbReference type="OrthoDB" id="1232at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140671">
        <property type="term" value="C:ADA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019340">
        <property type="entry name" value="Histone_AcTrfase_su3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13556:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13556">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10198">
        <property type="entry name" value="Ada3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="79"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="386"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="632" mass="71452" checksum="48E8B2DBD167BEE7" modified="2011-11-16" version="1">MAESHSASTTLDDIITELRLSYDSTVGLLDGEAVRTIPNVNTLLNLNKLLTKLWNGLNEVEESDDTALAAINALEAEKKKAEEMEEQDSSVNEKKRARESSISEEGEFQKENVEEVVGEEEGEDSINLAKRRKLSSGASAADEDEDDKKQKEEDEEEEHDDKERVNSKDDPVPPIQSGSFTQDNDTRLKNPKSEYVEPQTLSAEAIAELGLFSEAKFGSDEVPDREYLKKKYGVASFPENDLQDLLPGQIPDIDFSKNKPPTNQVQFSTFQSYIEPYFRPFSTEDIEFLRERNIIPPGFNKQDYDADVTPFLIPKLGRFYADVWAEEDATLSSKLNSPAFQQPQSDSYKPKGSIDVISDDVLYTEEISCGPLSSRLLSAILSTHEGTLQDEDPEQPNGERPASESLDDEQIATQLDSTEDYKVTTEVNDFQSIEERLKRELKYIGIFMNLPLTSEDGKIKTKQGGRVPKKLTASIIDNDEWIKNKEDDEVCAEIRSLQKELKETVSRNRANRKKLIPLVEEHIAYQEYCAILEDLDKQIGQAYMKRMKGKSKKKKVEANTPQQQALNNGLRALLEKRRRWIENIGKLFPNPEVMKRVPAESILVKEGEVDPEPAEDENTDNVSTAVEIIQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFI4</accession>
    <name>G3AFI4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Palmitoyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_58097</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="11"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372385.1">
        <property type="nucleotide sequence ID" value="XM_007372323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFI4"/>
    <dbReference type="FunCoup" id="G3AFI4">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="GeneID" id="18872320"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58097"/>
    <dbReference type="eggNOG" id="KOG1311">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047581_1_0_1"/>
    <dbReference type="InParanoid" id="G3AFI4"/>
    <dbReference type="OMA" id="CGTCHIW"/>
    <dbReference type="OrthoDB" id="9909019at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031211">
        <property type="term" value="C:endoplasmic reticulum palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF43">
        <property type="entry name" value="PALMITOYLTRANSFERASE APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="305"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="13">
        <location>
            <begin position="217"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="407" mass="47239" checksum="A75BDE0A8271633C" modified="2011-11-16" version="1">MTVQGVSYSPTQPVSPQRSIGESTGSTAAETIPSSHASKATPTSNDPPKLSLLHKFITNWLIMDPALLDKYKHTYEENVVNSSTRQSRRRRHIRNYQIQKHKDMKYIYFFGGRFRTIKAMPITILTGAAIVIPSVLFWIFEASWIWHNIHPAAVIMLSYICWLCFMFYFKSSTSDPGVVPRNIHIPKSLTSNTVKLPPEEYFNTISLPGHNHCKVQVKYCPTCHIWRPPRTSHCSVCQACIISHDHHCVYLNNCIGERNYRYFLWFLLTAVLSCLYMLIITIVHLCYYRIVSSDITTFGHSVKKYPVALLLFIYSVLALIYPFLLLLFHIFLTAQNLTTREYLNYVYKRRNNTYVNVFDTHNIFKNLYINWLGKSSGVALVRPRDKYEPGDLRFEQVLPLELFTKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG09</accession>
    <name>G3AG09_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1479-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_133205</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372560.1">
        <property type="nucleotide sequence ID" value="XM_007372498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG09"/>
    <dbReference type="STRING" id="619300.G3AG09"/>
    <dbReference type="GeneID" id="18869728"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133205"/>
    <dbReference type="eggNOG" id="ENOG502QRVA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011148_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG09"/>
    <dbReference type="OMA" id="YIMLRPL"/>
    <dbReference type="OrthoDB" id="8249012at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010856">
        <property type="entry name" value="Gig2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30613:SF1">
        <property type="entry name" value="DUF1479 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G09280)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30613">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YBIU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07350">
        <property type="entry name" value="Gig2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="466" mass="53455" checksum="6638FB7A9B756187" modified="2011-11-16" version="1">MSPSKLTDNTEPVDLESRFVQVKQRLIKPENVEKVTESWKKLLIEIESEFARIAKAGPSYVPRCDFASIKDQKLPKDISDLFKQRGVVMIDNVIDEQQIDIWFNELVDFCKEHPETAGYTFPNPTAWYNVFWTKPQSEARFHPNMKKLVATMAQEFYVENKSTLLDLDTQIVYGDRIRIREPGASATLPLHLDSSSIERWEDKEYSKVYEQIFSGNWEDWDPFKLDNRAHANENLYEHIHEGKSTICSAFRTLQGWLALSNNKSGEGTLRMLPNIKLSMAYIMLRPFFWKDPESGDINDYEIDLSTPKFPGAVPSTGQLFLQDFYHHLHQGVISIPDVKKGSFVYWHCDIPHEVDREHNGDGPSSVFYYGQCPLSVINIKTLLDTKQAFLNNVSPEDYRSQLSEDDKKKEFQGGDIAHLQDDPDAKRSMGLLPFEITDSLTEGQVKVRQIANEALANGSVDVSNYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG52</accession>
    <name>G3AG52_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosomal RNA-processing protein 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_58387</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRP17 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372603.1">
        <property type="nucleotide sequence ID" value="XM_007372541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG52"/>
    <dbReference type="FunCoup" id="G3AG52">
        <property type="interactions" value="244"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG52"/>
    <dbReference type="GeneID" id="18872431"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58387"/>
    <dbReference type="eggNOG" id="KOG4709">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067149_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG52"/>
    <dbReference type="OMA" id="EWDGFPD"/>
    <dbReference type="OrthoDB" id="551633at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000477">
        <property type="term" value="P:generation of mature 5'-end of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019186">
        <property type="entry name" value="Nucleolar_protein_12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14577">
        <property type="entry name" value="NUCLEOLAR PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14577:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09805">
        <property type="entry name" value="Nop25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="67"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="67"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="231"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="28779" checksum="D031802545F13ACF" modified="2011-11-16" version="1" fragment="single">MVQRNREILTGGKKYAQKAAKKHLVDEVVFDKESRKEYLTGFHKRKLQRQKKAQDYIKEQERLRRIQERKEMRDERKRDMENQLKAFNKTMKDITVGESDEEDQDWNGFEEGKGDEEEEEDDDEEEEDDEEEQPLKGILHHQEVYKVDNEELLSNSVIDEETTVTVESLDNPVIESAKQANLEAIARANNVHLEKSEAVLEKSIKKAKNYAVVCGVAKPERKPKKKFRYLSKAERRENTRKEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG92</accession>
    <name>G3AG92_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_130666</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372643.1">
        <property type="nucleotide sequence ID" value="XM_007372581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG92"/>
    <dbReference type="STRING" id="619300.G3AG92"/>
    <dbReference type="GeneID" id="18869512"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_130666"/>
    <dbReference type="eggNOG" id="KOG0531">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020128_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG92"/>
    <dbReference type="OMA" id="DMDTVYI"/>
    <dbReference type="OrthoDB" id="266138at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050836">
        <property type="entry name" value="SDS22/Internalin_LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652">
        <property type="entry name" value="LEUCINE-RICH REPEAT AND IQ DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652:SF3">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="687" mass="77384" checksum="AAA127306A06C97B" modified="2011-11-16" version="1">MAEGNGIAAPVFSHEYEDKAIGEPSPKRIKTEDTSEVEITQTISSPDVKTIETVNNHNQTTQDNPTEVKLPTVSNSQSPIVLPIPTPQNNNTSIPITATTTTTTTTAPAPAPAPPPQQKESVINTNSTTTLPPHLPPPIPVPPLEIKKINRLVLLYDKPRYYHTIIQLKELRELPNNYVTVLEIGTIDKVLKETTISSVKFDHFHMSIEYNDQFESNLRKFIEFLKNNQKMIDEVGDIAYHIHFEPGKKWQDYKPSEIKEYYNFLEALKDTAGDKVVHCSVVNKYDMDTVYITEKAELTKLGNEIQEDIQYWKNLKILDYGESSIRFLPGVKLPDTLEVLNIGGGYALETLTGFKMPPNLRALLAGQGAVHSIDNITFPTTLERLEIEDNKIYFLNYVEFPASLKHLDVSQNRIESLRGVNFPRGLQSLNIGFNPIESVRGIKFPETLRKLEVSNIPNESMTGVKFPESLEVLNLQSSMTNTRGLKLPQHLKTLILTGNGVNSINPLKLPNTIEVLYLNQNNIKTLNKVQLPPKLRELYLGDNLITTLKNVTFPSTLEVLDLENDPESIENDKHITTLKDVVLPPNLKIMKLGYHAIKAIESYEFPTSLRFLSLAYNELRQIKNIKFGNHLKTLDLSGNPELLSLDNVIIPESVTELRISPQLIGNLPAYIIERANKNRLIIKKSAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGG7</accession>
    <name>G3AGG7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SPX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_146408</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372718.1">
        <property type="nucleotide sequence ID" value="XM_007372656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGG7"/>
    <dbReference type="FunCoup" id="G3AGG7">
        <property type="interactions" value="125"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGG7"/>
    <dbReference type="GeneID" id="18870715"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_146408"/>
    <dbReference type="eggNOG" id="KOG1281">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005170_8_0_1"/>
    <dbReference type="InParanoid" id="G3AGG7"/>
    <dbReference type="OMA" id="DINNFPW"/>
    <dbReference type="OrthoDB" id="10260443at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006797">
        <property type="term" value="P:polyphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01115">
        <property type="entry name" value="SLC13_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14478">
        <property type="entry name" value="SPX_PHO87_PHO90_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF110">
        <property type="entry name" value="INORGANIC PHOSPHATE TRANSPORTER PHO87-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="498"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="530"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="578"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="628"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="668"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="711"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="756"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="779"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="811"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="847"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="890"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="934"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="8">
        <location>
            <begin position="1"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="115"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="285"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="962" mass="107102" checksum="65C4134953C0BD18" modified="2011-11-16" version="1">MKFEHSLKFNAVPEWQDSYVNYPTLKKTIYKLQQDQIVNNGTGESGFIVGSNQTTVTELVDGFKSKSNSQGVQEKSRAATMQGGLKNRLVKSLFLQRFQKGSSSTEIETNKVEVDSDLEKNSSSEYTEKGSDTKTYTEEGFDSDSIASFNPRKSTDVIGRILASEDDSFADPKSLKFDALKVFAKQLLVELDKINTFYNNKEEEVFTNYDNLLKDLESNHVDIDDVFRFTQAYNMNQAALNTDDHHQYHLKSTLSRVVSNASVFDTINHLGNDFDNKDLEKNEQADEEFDDSDEDDDEEDAHSEDSVLLNHAHFNVKHQLKVTLKRKAILLFISLSELKSYIELNRVGFTKICKKFDKTCNYSIKQDFIENFLPNHSRVFLKETIENLDYKLDHIVKVFAFLSGRLNSKTSVEDLEHVKVDLRSHLRDHIVFERNTVWKDLLSLEKKSYDIDLQANGQKMGDEANENSMLYITMKDFYFPKNIKIFGHDHVRLPASMITWQVFKLLLIIVVFVVLIAVKTFNDPVQGRALALLVACAMLWALEALPLYTTGMFVPLLVVTCRVCKKTGSNEPMAAADAAAYILGTMWNSTIMILIGGFTLAAALSKYNIAKVLSSYILAFAGTKPRNVLLAIMCVSLFLAMWISNVAAPVLCFSLIQPVLRTLPTTSPVSQALVLGIALASNVAGMASPIASPQNVIALQYMIPNPGWGNWFAVALPVSIIAMLLIWVELILTFKIGNVTLKKYKPIKEKFTAKQYFVFAVTLLTILLWCVMTKIEDTFGVAGIITIIPIVLFFGTGLLKVDDLNNYPWSIVLLAMGGIALGNAITSSGLLSTIAMAMERRIREFPLYAIVVIFGALILVVATFVSHTVAALIIVPLVKEVGDSLPHPHPLILVMATALIASGAMGLPTSGFPNVTAISMRDEVGKNYLTVNTFITRGVPASIIVYICVITIGYGVMSSLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGK0</accession>
    <name>G3AGK0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_48344</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase type-B-like family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="3"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372751.1">
        <property type="nucleotide sequence ID" value="XM_007372689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGK0"/>
    <dbReference type="FunCoup" id="G3AGK0">
        <property type="interactions" value="651"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGK0"/>
    <dbReference type="GeneID" id="18871206"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48344"/>
    <dbReference type="eggNOG" id="KOG1906">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013572_5_0_1"/>
    <dbReference type="InParanoid" id="G3AGK0"/>
    <dbReference type="OMA" id="IIKQFLH"/>
    <dbReference type="OrthoDB" id="273917at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:histone mRNA catabolic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071042">
        <property type="term" value="P:nuclear polyadenylation-dependent mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071036">
        <property type="term" value="P:nuclear polyadenylation-dependent snoRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071037">
        <property type="term" value="P:nuclear polyadenylation-dependent snRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043634">
        <property type="term" value="P:polyadenylation-dependent ncRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000006">
        <property type="entry name" value="non-canonical poly(A) RNA polymerase PAPD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000003">
        <property type="entry name" value="non-canonical poly(A) RNA polymerase PAPD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002058">
        <property type="entry name" value="PAP_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF15">
        <property type="entry name" value="INACTIVE NON-CANONICAL POLY(A) RNA POLYMERASE PROTEIN TRF4-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03828">
        <property type="entry name" value="PAP_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="10">
        <location>
            <begin position="184"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="PAP-associated" evidence="9">
        <location>
            <begin position="376"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="567"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW35339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="615" mass="69799" checksum="75ABCC4455D24315" modified="2011-11-16" version="1">MGSKRKREVSGIKPKSKKQKKKQLKTLQNSLTPGHNKFEKLANLNGNHDGDEEDSDSSVIFDFSREGSKESEIVLVDSDEEEEGKSDIVVISDTDNKENTDASTEPPQKKRKVEGNELTNNEDFIGFGFSSSSDEAEDNFDDLSDDGVLSADEQGIIIDGPKKSLYPWIKDHDHSTQKEIADWLTMEIKDFVDYVSPSSDEIVTRNTVVNRLKTQIAKFWPGTEAHVFGSCATDLYLPGSDIDMVVISETGDYENRSRLYQLSSFLRSKKLAKNVEVIANAKVPIIKFVDPESEIHIDVSFERTNGIDAAKRIRKWLITTPGLRELVLIVKQFLRSRRLNNVHVGGLGGYATIIMCYHFLRLHPKVSTGSIDILDNLGVLLIEFFELYGRNFSYDNLIISLDPNTEEPRYLTKGTNAVLNTARSTFSIVIQDPADPRNNITRSSYNLRDLKKAFGGAYQLLVEKCYELNGASYKDRLGQSILGDIIRFKGKERDFNDDRHKVVNTALVNHESEEEDEESDGLEGNDKYYFSDMTVESDTDLDAKYIPKEPTPVTNKAKDTKKLVESFLSLEDTENDDKYEPKEPEEDDAVEIKRSKSNLDKEIRRDYWRQKGLDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGM6</accession>
    <name>G3AGM6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CWH43-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_130726</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372777.1">
        <property type="nucleotide sequence ID" value="XM_007372715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGM6"/>
    <dbReference type="FunCoup" id="G3AGM6">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGM6"/>
    <dbReference type="GeneID" id="18869523"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_130726"/>
    <dbReference type="eggNOG" id="ENOG502RZQS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050573_1_1_1"/>
    <dbReference type="InParanoid" id="G3AGM6"/>
    <dbReference type="OMA" id="IYTFYVW"/>
    <dbReference type="OrthoDB" id="10032492at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019402">
        <property type="entry name" value="CWH43_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050911">
        <property type="entry name" value="DRAM/TMEM150_Autophagy_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21324:SF2">
        <property type="entry name" value="EG:22E5.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21324">
        <property type="entry name" value="FASTING-INDUCIBLE INTEGRAL MEMBRANE PROTEIN TM6P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10277">
        <property type="entry name" value="Frag1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="183"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="CWH43-like N-terminal" evidence="6">
        <location>
            <begin position="12"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="272" mass="31141" checksum="0318F857685C723C" modified="2011-11-16" version="1">MKTNRVRFTIIHWYLLPLVSLVVWWGMLIALLACWGAQGRPIYSFMTKYQNPVYISSIAATNLQPLFIACSGFQAIFFVCTLVVAYYLRTHGKIQSYISKVQQRFAIVSMVFATIGQLGILFTSIFNTSAFPKVHISFVGIFIICVFLACVFDFLISFMFGTHPDKLDPHHDFVIFGHHKWSNLYMVAFGLKVVWVATAVALAVCFGYYMRIGHKSRSAIFEWVIAFWYGLLLIMWSMDLLSSAIRSYERKHNTEFDKTEITCSGSNDSTFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH77</accession>
    <name>G3AH77_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_64619</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372918.1">
        <property type="nucleotide sequence ID" value="XM_007372856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH77"/>
    <dbReference type="GeneID" id="18874986"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64619"/>
    <dbReference type="eggNOG" id="ENOG502RQK7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1384936_0_0_1"/>
    <dbReference type="InParanoid" id="G3AH77"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="197" mass="23749" checksum="FC81DAD892368EF2" modified="2011-11-16" version="1">MLQIWSTHFSENFSFLLLSSFSKPGTMTLSIDNYIFEELEEMYNDAVAELLISYSEFLQLQHKQSFTLCKILFTNIKSRKKYFENKWILTQIHLFKKNAEVWEWRKVVNVILATMKDKGFISSRELRRLHSGLVREFKHVEEKFEAHRAKMLNENKDVAIVLSLLQEYPRVGKHLKQLEKSLLWRLRIMNREERRTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHA5</accession>
    <name>G3AHA5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_48520</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372947.1">
        <property type="nucleotide sequence ID" value="XM_007372885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHA5"/>
    <dbReference type="GeneID" id="18871235"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48520"/>
    <dbReference type="eggNOG" id="ENOG502SGGK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_445613_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHA5"/>
    <dbReference type="OMA" id="TIRCCAM"/>
    <dbReference type="OrthoDB" id="4065753at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="613" mass="70363" checksum="87936B81B15B16C1" modified="2011-11-16" version="1">MIKYPSSIVIGDAIQPVSSNEFDSSFNSQTSFDSKSYTKSRQKSVFAKLEHFYNVDNMIEILYLLPELKHIDSFPPHIHVIVGSTLFKSGMTLEGLREVGVAIDMERDRSVRADHISLMGTMLARLGKMDDAMTCFGDYFDDVRNSNGDVGEIKQKVRGFQDELARIGAGLEYVIFCDLRLKVFTNISRIIPSFPSLEQQILNFKDVHKIFPMGSFTYDYSHIVKIISDVKQRLETWDYTKKKGDQSDNSVILSLSTAILILGRVEIYYSMLAIEPLILRHIEAFVPPTQQHTRTYSLFKKKPELPTIPYEVKIAQRKTVIGFLALLNHDYSRAVQEFTNSIELLDPQDSMCDQVKCWLIQCKCMMETNPKNRLAAIMDIFEDRPHPKIYIALGDCYRDLAGEESTSITILEPNKIVIGKQFLCSHLEEAIFNYINTVVSMQQDDPAVAKIYDYIVLYLLLHGGIQYHAFAFFLQLRDYFHVISQYNHLHLSNDDSTYTNFFEIADQFVQDSKLLTGDFWRPKAINLLPQVFLAEDDKLLVMKGKYGVATTTKFKTVNSFYIVPVDHEPLISSIALLESKILTSRTLVDLFWRGYSGDLPSHIQQYYIQHLAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHP1</accession>
    <name>G3AHP1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mannosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_135420</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373789.1">
        <property type="nucleotide sequence ID" value="XM_007373727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHP1"/>
    <dbReference type="STRING" id="619300.G3AHP1"/>
    <dbReference type="GeneID" id="18869888"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135420"/>
    <dbReference type="eggNOG" id="KOG4472">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024327_4_2_1"/>
    <dbReference type="InParanoid" id="G3AHP1"/>
    <dbReference type="OMA" id="WSYPSHI"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF6">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442333" evidence="7">
        <location>
            <begin position="25"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="390" mass="46727" checksum="BB82F0A515818198" modified="2011-11-16" version="1">MWRSVSLIVTVLAILLSLPRIVKVNDELDNSTKLIDGLYYTYCNDSDFKDFLKNNEIFSAEVNQDIPSQVVIIDREKPRTPRENATFLMLCRNQDMYQVLETLQNVQDRFNHKYNYDYTFLNDEPFSKEFIYLITSFIDHGRISFGSIPRSHWSYPSHINTTKAEEIRSNYADVPYGDSESYRHMCRFYSGFFYQHPLVKQYQYYWRIEPGIKVYCDVNYDVFRYVRKHKKKYAFVLSLFEYADTIPSLWNHVLEFIRTENMTSAPESFELLLNKHNWYNLCHFWSNFEIANLDMFDNNYQRLFEFLDSKGGFYYERWGDAPIHSIAVALYLYKNEIHWFNDLGYYYAPYSQCPQDSKTFIENKCTCDPDDDFGQSDLSCNRYFLDILSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHW3</accession>
    <name>G3AHW3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-rRNA-processing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_54434</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Component of the RIX1 complex required for processing of ITS2 sequences from 35S pre-rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the RIX1 complex, composed of IPI1, RIX1/IPI2 and IPI3 in a 1:2:2 stoichiometry. The complex interacts (via RIX1) with MDN1 (via its hexameric AAA ATPase ring) and the pre-60S ribosome particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the IPI1/TEX10 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373861.1">
        <property type="nucleotide sequence ID" value="XM_007373799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHW3"/>
    <dbReference type="FunCoup" id="G3AHW3">
        <property type="interactions" value="256"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHW3"/>
    <dbReference type="GeneID" id="18871860"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54434"/>
    <dbReference type="eggNOG" id="KOG2149">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050252_2_0_1"/>
    <dbReference type="InParanoid" id="G3AHW3"/>
    <dbReference type="OMA" id="CAGGWVK"/>
    <dbReference type="OrthoDB" id="361362at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120330">
        <property type="term" value="C:rixosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030174">
        <property type="term" value="P:regulation of DNA-templated DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024679">
        <property type="entry name" value="Ipi1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16056">
        <property type="entry name" value="REGULATOR OF MICROTUBULE DYNAMICS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16056:SF2">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12333">
        <property type="entry name" value="Ipi1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 7" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Pre-rRNA-processing protein Ipi1 N-terminal" evidence="8">
        <location>
            <begin position="135"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="351" mass="39078" checksum="48A12B425007964B" modified="2011-11-16" version="1">MGSKKKKADKKKDFVKPKLKVGKTAAKPDNYTDTSFIAKTISLPNQSIAKVTSGTGSTNKSDIDLSHHLSLTKHHSSTTRKEVLIYIQQHLPSNPSTYSEILKTVIPLILDESQQVRTALIDLLKSIGDKQPGLLDLHLRSIILFILSAMTHIQPSIRHSSTKFLNILVDNCINSLVGSYFIKILKSFFSLMGWTLVNDKKQVSIAITTTSIEGSSKKARIGHLQVLRNLLQKSLFQEETLDIDIENIISIHPQSYRYIIPATPNPFASLKLFVNEMPKQKGDVSLGEDSYTVNDLDSISTEDLHTRRKIVVDVFKPPMVKHLNNLIKEGGDVGREANSCLLLLEDLSKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI05</accession>
    <name>G3AI05_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_54472</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373904.1">
        <property type="nucleotide sequence ID" value="XM_007373842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI05"/>
    <dbReference type="FunCoup" id="G3AI05">
        <property type="interactions" value="764"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI05"/>
    <dbReference type="GeneID" id="18871866"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54472"/>
    <dbReference type="eggNOG" id="KOG1716">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023312_0_1_1"/>
    <dbReference type="InParanoid" id="G3AI05"/>
    <dbReference type="OMA" id="FAWQGMQ"/>
    <dbReference type="OrthoDB" id="2017893at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000786">
        <property type="term" value="P:positive regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14518">
        <property type="entry name" value="DSP_fungal_YVH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016278">
        <property type="entry name" value="DUSP12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45848:SF4">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45848">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000941">
        <property type="entry name" value="DUSP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="72"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="5">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000941-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="328" mass="37466" checksum="F6FCD7C6FECEBE91" modified="2011-11-16" version="1">MSVYRILGGIYLSSIEPLNSGVNLKRDYGIHHILSVIPGPIPAAYTSEYHWKQIEITDEETTNLFPYLEPCYKFIDEALFSESTDPKKHSDNILIHCSQGVSRSVAIVMSYLMKKYKLNVQQSLHAVKRKCPDVGPNEGFVSQLKLYKDMGCVVDEDNDEYRQFLVDLNLKLDPSGQSLRELMSKRSESTAPQETEVVYELRCKRCRQVLANNTHIEQHEIPVAESRQSQFVKTAPNSRRVISIEEASDKCSHYFMKDPLKWMKEELDKSEIEGKFQCPKCTSKVGGYSWRGSRCSCGKWMIPAIHLQQAKTDCISKNLHAPIHQSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIA7</accession>
    <name>G3AIA7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59046</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373260.1">
        <property type="nucleotide sequence ID" value="XM_007373198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIA7"/>
    <dbReference type="FunCoup" id="G3AIA7">
        <property type="interactions" value="228"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIA7"/>
    <dbReference type="GeneID" id="18872739"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59046"/>
    <dbReference type="eggNOG" id="KOG3035">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051989_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIA7"/>
    <dbReference type="OMA" id="KMQQFPG"/>
    <dbReference type="OrthoDB" id="671439at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01838">
        <property type="entry name" value="Isoamyl_acetate_hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045136">
        <property type="entry name" value="Iah1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14209">
        <property type="entry name" value="ISOAMYL ACETATE-HYDROLYZING ESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14209:SF19">
        <property type="entry name" value="ISOAMYL ACETATE-HYDROLYZING ESTERASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH hydrolase-type esterase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="270" mass="30888" checksum="051312159381A584" modified="2011-11-16" version="1">MPGLRYDKFILFGDSITQFSNSQQDGFAFQPAIQDLYQRKLDVINRGYSGYNSNHAREILPEILKAELNSAKDNVKLLTIFFGTNDAFQIEDETNKVQSVEVEKYKENLNAMVELALANNIKPVIVGPGIHDCKRVRLFFTDRPTEKAPVTNKRLLDYSNAAKEVAAKHKVAFVDTWNAFREYGGWTEQQLFDATGLGEWEVGTLEHLVPDGVHFSPLAYKILYEKLIEAIDKNYPELNADKLPEKLSYWRDLDPSNYKNLIFNKGDLDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIE7</accession>
    <name>G3AIE7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_59849</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. MetX family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374001.1">
        <property type="nucleotide sequence ID" value="XM_007373939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIE7"/>
    <dbReference type="FunCoup" id="G3AIE7">
        <property type="interactions" value="144"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIE7"/>
    <dbReference type="GeneID" id="18873101"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59849"/>
    <dbReference type="eggNOG" id="ENOG502QRIX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028760_5_0_1"/>
    <dbReference type="InParanoid" id="G3AIE7"/>
    <dbReference type="OMA" id="LGGCQGT"/>
    <dbReference type="OrthoDB" id="191364at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004414">
        <property type="term" value="F:homoserine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009092">
        <property type="term" value="P:homoserine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00296">
        <property type="entry name" value="MetX_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008220">
        <property type="entry name" value="HAT_MetX-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01392">
        <property type="entry name" value="homoserO_Ac_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268">
        <property type="entry name" value="HOMOSERINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268:SF11">
        <property type="entry name" value="HOMOSERINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000443">
        <property type="entry name" value="Homoser_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="5">
        <location>
            <begin position="61"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="250"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="267"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="394"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000443-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="487" mass="54838" checksum="89164B172B93AC26" modified="2011-11-16" version="1">MVPCKYKDVTEEQRQTNSFARLVPGQTIVEIPEYTLECGETLYNFPVAYKTWGRLNKERDNVLLICHALTGSSDVQDWWGPLLGNGKTFDPSRFFIICINFMGSPYGSCSPVSIDKRTGKPYGPSFPLVTVKDDIGIQKLILDSLDVNGISCVIGGSMGGMLALEYSATYNDSNYVKTVIALATSARASAWCISWNETQRQCIFSDPYYDDGYYYENGSGVKPDSGLSAARMAALLTYRSRNSFETRFGRTVGTKKAAKPEPSNLSEEERLKREEAEKGIRYPKTKDEENWLLHNEGSRSSLNIPALANGSSKPNNKKPPTYFTAQSYLRYQGNKFISRFDANCYISISRKLDTHDITRGRIEPADHYARDQDPLPDYLQTLRKPHLVIGIQSDGLFTFGEQQLLGDNIPGAILKKLDSPEGHDAFLLEFELINGYCLDFLQKSLPEFYDEKSGKFELFNNWQDFVEDIDNGGNSVFGEAEKNITNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AII9</accession>
    <name>G3AII9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 30 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_59071</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373288.1">
        <property type="nucleotide sequence ID" value="XM_007373226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AII9"/>
    <dbReference type="FunCoup" id="G3AII9">
        <property type="interactions" value="116"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AII9"/>
    <dbReference type="GeneID" id="18872756"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59071"/>
    <dbReference type="eggNOG" id="KOG4813">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085412_0_0_1"/>
    <dbReference type="InParanoid" id="G3AII9"/>
    <dbReference type="OMA" id="PRERFNK"/>
    <dbReference type="OrthoDB" id="20381at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.60">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023194">
        <property type="entry name" value="eIF3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013906">
        <property type="entry name" value="eIF3j"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08597">
        <property type="entry name" value="eIF3_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="224" mass="24725" checksum="2D2B1FA8159569F2" modified="2011-11-16" version="1" fragment="single">MSWDDEDFDIPAGNTAAAASWEDEGDDEPILESWDIDEDEIAKQKKEAADKAAAEKAAAKKKADEAAAKKAAKIKEMEQFDSLDEETRKDLLKKAELNSDLNNAADLFGGLGVAGDGDDFDINAHPRERFNKALLASQQRKPVLTKDTPLTDHPLFQPTNKAEYEKLRKAVGTSLTELNEDSSLLYASSLAIDLIRDLAQPLSVENLRKVISTLNVVIKEKERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIP1</accession>
    <name>G3AIP1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_65593</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374041.1">
        <property type="nucleotide sequence ID" value="XM_007373979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIP1"/>
    <dbReference type="STRING" id="619300.G3AIP1"/>
    <dbReference type="GeneID" id="18875049"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65593"/>
    <dbReference type="eggNOG" id="KOG0128">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_333463_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIP1"/>
    <dbReference type="OMA" id="LWARYIL"/>
    <dbReference type="OrthoDB" id="360390at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12296">
        <property type="entry name" value="RRM1_Prp24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12298">
        <property type="entry name" value="RRM3_Prp24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12299">
        <property type="entry name" value="RRM4_Prp24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034397">
        <property type="entry name" value="Prp24_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034540">
        <property type="entry name" value="Prp24_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031766">
        <property type="entry name" value="RRM_occluded"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF119">
        <property type="entry name" value="NUCLEAR RNA-BINDING PROTEIN SART-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16842">
        <property type="entry name" value="RRM_occluded"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="540"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="609"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="706"/>
            <end position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="908" mass="105306" checksum="D6EEFF9418FFA2AF" modified="2011-11-16" version="1">MSDSVEVTIESKITECEAFIDENPYVAENYDNLLDLLQVSSNPTKLHDVYKQKLTYFRLKQKELLNWYEILLKIDDDLQRASLQVEYFELILLDYPDVPTWKRYLDQCETLIKMEIIDDDDYRLILQRAMKDCFHDFENCHEIWNIMLSYFYDRYLKTKNQDHWDHLVQLHLSRLGQSHAQLDDSFSEFSSLISKHDAEHYDQQMTNANKIYSDTKRNQHYYEHYEIKIKSDPSNPQVYIDYIENVAKYESRSMIAIFNRAISAEYSWSPEWQPVWMTVIYQLYAHDEMNLLESLLKKFVRTFAESCRPYAELIRNCIEFDDGETLFNETKERIDNLDLMTKSDYSEWKLLASAIIQYRFNAKDNENLYVDALKYVEFAIDYNNDMLHSVEKLACSIFQKLGDLEEASKIVDLMVDRFNEQADVWLYMINYLTVNSESQETLRQTFQNALLFAAAMDAPEKIIEEWLIFEQANGDISSYRDAVLECNAALKFNLMNKVEQFETKKADEVAKVEKAEKKKKSGQKRKLSETQEPVRSREEFTVKVTNIDNVSKEDLIAMFQDCGEIREISVLENSALIEFTTDQSVLSALTKDHKRINDHEITVTKLGNSTLYVNNFPSTYTQSQIQDKFSTVGPIVSIRFPHQNNPNKTKRFCYVQYVNPDDAKRAIVLYNGEMLHDDQFNTDFKLQVMISRPQERKQRSSPISERKIRVSNLSFELGEDQIKDIFVKCGEIDSIVIPRKNKKYSNEGVAIIVFEGIESVDRALELNETEISGRKLHVERPRHHHQKEVTVKDFNKLSTIGLINVDTTLKPYDIKAYFEKFGAVEKSMVLPDSASALVEFASPTTAGTVSMSGDKIILGQTEATITEMSTAIAATAPATAPATAPTPVQSVKSTRIMIPASVKRRKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIR5</accession>
    <name>G3AIR5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_70588</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374065.1">
        <property type="nucleotide sequence ID" value="XM_007374003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIR5"/>
    <dbReference type="FunCoup" id="G3AIR5">
        <property type="interactions" value="723"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIR5"/>
    <dbReference type="GeneID" id="18875335"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70588"/>
    <dbReference type="eggNOG" id="KOG1188">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037323_3_1_1"/>
    <dbReference type="InParanoid" id="G3AIR5"/>
    <dbReference type="OMA" id="SIHSCGW"/>
    <dbReference type="OrthoDB" id="25131at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039328">
        <property type="entry name" value="WDR89"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22889">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22889:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="147"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="345"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="354"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="386"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="395" mass="45011" checksum="E35BFCD801CF224B" modified="2011-11-16" version="1">MRAQCLFSWSSLASNDNWILKLQNLPQTNSFVCSSSNGTIATYNLDQPSQTPTFQVKAHESSINSLIKLNDNDNYSIATCSTDGIKLWDLRSSTPIAQLSQGRNSSFLSLGFNGDNLLAAGTELMGVDAELHIWDLRNTKDVVRSFIDSHHDDITALEFHPTISNYLMSGSTDGYVNIYDLNQPDEDEALHQVINYSSVHSCHFITPSRISVLSHMESLLFHDLNDTNYEELKEPEFVDLGDVRQLWPDNEYVIEVDPSGFVAYGANSQRKLTVFPFNPTKETFDTTKPIWFPDAHGEEVVRDVCTIPNSKNALTCGEDGQIRLWELPYQLETYSINASETVDIDMDKEESEKKKKKDKKDKKEKKDKKEKKDKKDKKDKKKKDKKKGDVRFKPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIU6</accession>
    <name>G3AIU6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin-like 1-activating enzyme E1A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_59120</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373341.1">
        <property type="nucleotide sequence ID" value="XM_007373279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIU6"/>
    <dbReference type="FunCoup" id="G3AIU6">
        <property type="interactions" value="1204"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIU6"/>
    <dbReference type="GeneID" id="18872783"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59120"/>
    <dbReference type="eggNOG" id="KOG2014">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002556_4_1_1"/>
    <dbReference type="InParanoid" id="G3AIU6"/>
    <dbReference type="OMA" id="EFFGQFD"/>
    <dbReference type="OrthoDB" id="1708823at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000011">
        <property type="entry name" value="UBQ/SUMO-activ_enz_E1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF162">
        <property type="entry name" value="SUMO-ACTIVATING ENZYME SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01849">
        <property type="entry name" value="UBIQUITINACT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="9">
        <location>
            <begin position="14"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="346" mass="38615" checksum="D83C10A52E87A057" modified="2011-11-16" version="1">MTKDELSAQEIALYDRQIRLWGMATQLRLRSSKILIINLGAVGTEIVKNLVLGGLNTIEILDDSVVKPEDFAGQFFLPNDDSVVGQTKLPLVVDRIRELNNRVNLSINTESLDSLIANKQYVKAFDLVIATELDKQMILKLNDITRELNIPLYVSGMHGMFGYILTDLIEHTSVSVKEAGNQPRVVGTELSRNKTITNVESKEKSNDEIVTIRDVFSPLKEIFKSQELPKQLNKRQLKRLSGVVPLIFALFDITRPNDPDTVIDVEVLRVKAIEICNLLNIPAETVTPDYLDVFSRQAFAEFAPVAAVIGGALAQDIIQFLSKKESPINNVLILDAVNSEMPIYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIV9</accession>
    <name>G3AIV9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Geranylgeranyl transferase type-2 subunit beta</fullName>
            <ecNumber evidence="8">2.5.1.60</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_59130</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>geranylgeranyl diphosphate + L-cysteinyl-[protein] = S-geranylgeranyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11537"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:86021"/>
            <dbReference type="EC" id="2.5.1.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.60" evidence="8"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373354.1">
        <property type="nucleotide sequence ID" value="XM_007373292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIV9"/>
    <dbReference type="FunCoup" id="G3AIV9">
        <property type="interactions" value="311"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIV9"/>
    <dbReference type="GeneID" id="18872787"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59130"/>
    <dbReference type="eggNOG" id="KOG0366">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028946_3_0_1"/>
    <dbReference type="InParanoid" id="G3AIV9"/>
    <dbReference type="OMA" id="VKRCQCP"/>
    <dbReference type="OrthoDB" id="5428259at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004663">
        <property type="term" value="F:Rab geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02894">
        <property type="entry name" value="GGTase-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000028">
        <property type="entry name" value="Geranylgeranyl transferase type-2 subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026873">
        <property type="entry name" value="Ptb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF11">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="9">
        <location>
            <begin position="7"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="327" mass="37105" checksum="BB4D01C30CE36051" modified="2011-11-16" version="1">MTDVTFVKDKHIQYVIEQDSHRSFEYWLSEHLRMNGLYWGITTLATINSLDALPKAEVIDFILSCWDDKSGGFGSFPKHDSHILSTLSALQVLRIYDNELTMISSEKRTKLVKFIKELQLPDGSFQGDRFGEVDTRFVYTAISALSLLDELTKEIADPAVDFIMKCRNFDGSFGMVPGAESHAAQVFVCVGTLAITDNLHLINQDIKLASWLSERQVLPSGGFNGRPEKLPDVCYSWWVLSSLAILNKKHWVDLEKLEGFILSAQDLKEGGISDRPDNATDIYHTCFGITGLSLIDWKKYNFKEIDPIYCMPVEVTKNFKKWKNLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIW7</accession>
    <name>G3AIW7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">S-formylglutathione hydrolase</fullName>
            <ecNumber evidence="2 7">3.1.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_54067</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Serine hydrolase involved in the detoxification of formaldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>S-formylglutathione + H2O = formate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14961"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="EC" id="3.1.2.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the esterase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.12" evidence="2 7"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373362.1">
        <property type="nucleotide sequence ID" value="XM_007373300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIW7"/>
    <dbReference type="FunCoup" id="G3AIW7">
        <property type="interactions" value="688"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIW7"/>
    <dbReference type="GeneID" id="18871829"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54067"/>
    <dbReference type="eggNOG" id="KOG3101">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056472_0_1_1"/>
    <dbReference type="InParanoid" id="G3AIW7"/>
    <dbReference type="OMA" id="PSDCPWG"/>
    <dbReference type="OrthoDB" id="420518at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018738">
        <property type="term" value="F:S-formylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000002">
        <property type="entry name" value="S-formylglutathione hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000801">
        <property type="entry name" value="Esterase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014186">
        <property type="entry name" value="S-formylglutathione_hydrol"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02821">
        <property type="entry name" value="fghA_ester_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10061">
        <property type="entry name" value="S-FORMYLGLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10061:SF0">
        <property type="entry name" value="S-FORMYLGLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00756">
        <property type="entry name" value="Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0719">Serine esterase</keyword>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR614186-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="285" mass="31670" checksum="192E14BDFEE19804" modified="2011-11-16" version="1">MTFKTSATITQFGGKLLKLSHDSTTTHTPMDVNVFIPSSGDKIPVLVYLSGLTCTPNNATEKSFFQYFASKYGFALVFPDTSPRGANIAGEDDSYDFGSGAGFYVDATQDPWNRNYNMYSYVHKELLTELAKEFKQLDFTNIGITGHSMGGMGALTGFLSQPGKYKSVSAFAPISNPSVVPWGEKNFGNYLGANKDDWYKYDPTHLIKKYTHENQPSILIHQGLSDDFYEGQLKPENFVAASKEVGYKGGVDLNLVEGYDHSYFFISSFVEEHAKHHAKYLGLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ08</accession>
    <name>G3AJ08_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_59953</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374104.1">
        <property type="nucleotide sequence ID" value="XM_007374042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ08"/>
    <dbReference type="GeneID" id="18873149"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59953"/>
    <dbReference type="HOGENOM" id="CLU_2924184_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ08"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442432" evidence="2">
        <location>
            <begin position="22"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="61" mass="5616" checksum="1AE862EDDEFEF957" modified="2011-11-16" version="1">MQFKSIVVALTAACAVSAANASANATGNHSNATTTGKNGAAQLGAAGAVGAIAAGAVALLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJA0</accession>
    <name>G3AJA0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">nitric oxide dioxygenase</fullName>
            <ecNumber evidence="4">1.14.12.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_59223</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>2 nitric oxide + NADH + 2 O2 = 2 nitrate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19469"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.14.12.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2 nitric oxide + NADPH + 2 O2 = 2 nitrate + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19465"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.12.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.12.17" evidence="4"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373441.1">
        <property type="nucleotide sequence ID" value="XM_007373379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJA0"/>
    <dbReference type="FunCoup" id="G3AJA0">
        <property type="interactions" value="358"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJA0"/>
    <dbReference type="GeneID" id="18872826"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59223"/>
    <dbReference type="eggNOG" id="KOG3378">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_12_0_1"/>
    <dbReference type="InParanoid" id="G3AJA0"/>
    <dbReference type="OMA" id="KLERMCN"/>
    <dbReference type="OrthoDB" id="436496at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008941">
        <property type="term" value="F:nitric oxide dioxygenase NAD(P)H activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070458">
        <property type="term" value="P:cellular detoxification of nitrogen compound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071500">
        <property type="term" value="P:cellular response to nitrosative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046210">
        <property type="term" value="P:nitric oxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19754">
        <property type="entry name" value="FHb_fungal-globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06184">
        <property type="entry name" value="flavohem_like_fad_nad_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.490.10:FF:000003">
        <property type="entry name" value="Flavohemoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000034">
        <property type="entry name" value="Flavohemoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396">
        <property type="entry name" value="FLAVOHEMOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396:SF3">
        <property type="entry name" value="FLAVOHEMOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00409">
        <property type="entry name" value="PHDIOXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0216">Detoxification</keyword>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Globin" evidence="17">
        <location>
            <begin position="9"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="18">
        <location>
            <begin position="156"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="324"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW33857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="394" mass="45109" checksum="D8E856F1BF93199E" modified="2011-11-16" version="1">MTQQYETKELTPEQIKLILETVPILEQAGETLTQKFYQRMIGGYDEVKPFFNETDQKLLRQPKILAFALLNYAKNIEDLTPLVGFVKQIVAKHVGLQVKAEHYPIVGTCLIETMVEILPAEIATGEFIEAWSTAYGNLAKLLIDMEAAEYTKQPWTEFREFSVTKIEDECDEVKSVYFQPVDGKISIPKRGQYVCIRWKLPNAEFEKSREYSISQFPEENEYRISVRKLPDGKVSGYIHETLKVGDILRVAAPAGNFVYQEGESDVVLIAGGIGITPLISILDKALADDRNVNLLYANRTVKSRAFGDFLKEKKKQYGDKLNVIEFFDGEKVDQKDAIDQLENRMLDVKDLEFIDGEKHDVYLLGPRPFMRVIRDYLGKKDINVNLEYFGSYDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJD4</accession>
    <name>G3AJD4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoenolpyruvate carboxykinase (ATP)</fullName>
            <ecNumber evidence="3">4.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PCK1</name>
        <name evidence="11" type="ORF">SPAPADRAFT_60027</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>oxaloacetate + ATP = phosphoenolpyruvate + ADP + CO2</text>
            <dbReference type="Rhea" id="RHEA:18617"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphoenolpyruvate carboxykinase (ATP) family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.49" evidence="3"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374177.1">
        <property type="nucleotide sequence ID" value="XM_007374115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJD4"/>
    <dbReference type="FunCoup" id="G3AJD4">
        <property type="interactions" value="276"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJD4"/>
    <dbReference type="GeneID" id="18873185"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60027"/>
    <dbReference type="eggNOG" id="ENOG502QQI5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018247_0_1_1"/>
    <dbReference type="InParanoid" id="G3AJD4"/>
    <dbReference type="OMA" id="MRYAGEM"/>
    <dbReference type="OrthoDB" id="184182at2759"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004612">
        <property type="term" value="F:phosphoenolpyruvate carboxykinase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00484">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphoenolpyruvate carboxykinase (ATP)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphoenolpyruvate carboxykinase [ATP]"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.8.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00453">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001272">
        <property type="entry name" value="PEP_carboxykinase_ATP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013035">
        <property type="entry name" value="PEP_carboxykinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008210">
        <property type="entry name" value="PEP_carboxykinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015994">
        <property type="entry name" value="PEPCK_ATP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00224">
        <property type="entry name" value="pckA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006820">
        <property type="entry name" value="PRK09344.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006821">
        <property type="entry name" value="PRK09344.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30031:SF0">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE (ATP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30031">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01293">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006294">
        <property type="entry name" value="PEP_crbxkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68923">
        <property type="entry name" value="PEP carboxykinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53795">
        <property type="entry name" value="PEP carboxykinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00532">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="553" mass="60913" checksum="770844A6BE5FD2D1" modified="2011-11-16" version="1">MAPPPAAVESALDFGNKANIKSNQDPLVHQLSLNPDVIIRHNAAPPTLYEDGLTEKGTTISSTGALMAFSGKKTGRSPKDKRIVEESTSAHNIWWGPVNKQVNELTWKISRSRALDYLRTREKLFVVDAFAGWDPRYRIKVRIICARAYHALFMTNMLIKPTPEELENFGEPDFTVYNAGQFPANVHTKGMTSSTSVEINFKDMEMVILGTEYAGEMKKGIFTVMFYLMPIKHQVLTLHSSCNQGQDDDISLFFGLSGTGKTTLSADPKRKLIGDDEHCWSDQGVFNIEGGCYAKCIDLSAEKEPEIFNAVKFGAILENVAYDPETKVVDYSDSTITENTRCSYPVEFIPSAKIPCLADVHPSNIILLTCDASGVLPPVSKLTNEQVMYHFISGYTSKIPGTEEGVTEPIAVFSACFGQPFLVLHPMKYAQQLADKISQHKARAWLLNTGWVGASASTGGKRCPLKYTRAILDAIHSGELAKAEYETFPVFNLNIPKAIEGVPSEILNPARSWTKGTDSFNAELSKLAGKFVENFKTYADEATPEVKAAAPTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJH6</accession>
    <name>G3AJH6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">N-glycosylation protein EOS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_148062</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373463.1">
        <property type="nucleotide sequence ID" value="XM_007373401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJH6"/>
    <dbReference type="FunCoup" id="G3AJH6">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJH6"/>
    <dbReference type="GeneID" id="18870774"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_148062"/>
    <dbReference type="eggNOG" id="ENOG502QTWB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043059_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJH6"/>
    <dbReference type="OMA" id="HIWIFIS"/>
    <dbReference type="OrthoDB" id="2139606at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021100">
        <property type="entry name" value="N-glycosylation_EOS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28147">
        <property type="entry name" value="N-GLYCOSYLATION PROTEIN EOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28147:SF1">
        <property type="entry name" value="N-GLYCOSYLATION PROTEIN EOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12326">
        <property type="entry name" value="EOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02070">
        <property type="entry name" value="NGLYCOSEOS1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="181"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="349" mass="38729" checksum="EA6C5BADEA077AB4" modified="2011-11-16" version="1">MPLMDSLRNRFSDNLEANQIDDDYSVPARSSSSTALATGVASSQPEQTTSSDTQFDPDEVVPSYETSQRIYGTTQTINSASGIYTPIPSLPIPLTISDSNSSSSSSINSIPTTTHPHPNPQAPPSLKQLGLKFLNARQHFALALCRDISLMPALISLYQSWKRMFIHDSLLTSARGSEHFLAGLWCIVASYLSYSVLDGLIVRWIVIYSTPAAIVRVLSMSVIIIAVEFYLVSAFSAQGYKYGLHIWILISCILTFAYIIQNFFTSNLELRRIGGAGHTRSSSRTIEASEGRNNERKKERRRFFDFYNIVVFAVVPVGLASFVTMIGLLRSLLILRIDIETVARETKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJP3</accession>
    <name>G3AJP3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_59332</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373528.1">
        <property type="nucleotide sequence ID" value="XM_007373466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJP3"/>
    <dbReference type="STRING" id="619300.G3AJP3"/>
    <dbReference type="GeneID" id="18872871"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59332"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_714036_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJP3"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990526">
        <property type="term" value="C:Ste12p-Dig1p-Dig2p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990527">
        <property type="term" value="C:Tec1p-Ste12p-Dig1p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002817">
        <property type="entry name" value="Transcriptional regulator MNL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052127">
        <property type="entry name" value="STE12_transcription_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47427">
        <property type="entry name" value="PROTEIN STE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47427:SF1">
        <property type="entry name" value="PROTEIN STE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="316"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="345"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="69"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="172"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="213"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="387" mass="42931" checksum="E3A90208BAD53C99" modified="2011-11-16" version="1">MSPTKEEGQFDTLENMPVITKKPKRSKRSLFTRFKNPTSKLDAPIEDNATNIPPVAEESKNDIESASNSIIGNTSTHGSSIGGSTPSISGVNVMDSSASEASHQQSQLQSSEPDYAALFENVGKRKNITYRKPKPKVKDESLSTETSNTAGEQPTSSSLLNFGRSRSKEGSARSSIKQDNVSEQFSGENLSVASSNVSSTNQDLSHESEQQDSSTPSTPTTSTIASASKRILGSKLMPKKKSASKQLSAKDAVLLSLDTPVATMISKGVEVEVDLRSLDLPPDTKIFPTSIINSKNRTRGRKENKEADMVDETKIYLCNYCSRRFKRHEHLKRHFRSLHTFEKPYDCSICHKKFSRSDNLNQHLKIHKQEEEMAHMDQMEEVEVEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJT5</accession>
    <name>G3AJT5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_49074</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HRD1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373570.1">
        <property type="nucleotide sequence ID" value="XM_007373508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJT5"/>
    <dbReference type="FunCoup" id="G3AJT5">
        <property type="interactions" value="230"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJT5"/>
    <dbReference type="GeneID" id="18871300"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49074"/>
    <dbReference type="eggNOG" id="KOG0802">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_497911_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJT5"/>
    <dbReference type="OMA" id="SDNLCII"/>
    <dbReference type="OrthoDB" id="7759664at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16479">
        <property type="entry name" value="RING-H2_synoviolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050731">
        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057992">
        <property type="entry name" value="TPR_SYVN1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024766">
        <property type="entry name" value="Znf_RING_H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058051">
        <property type="entry name" value="Znf_RING_synoviolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763:SF184">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SYNOVIOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25563">
        <property type="entry name" value="TPR_SYVN1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12678">
        <property type="entry name" value="zf-rbx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="388"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="233"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="449"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="237"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="251"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="476"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW33986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="618" mass="69247" checksum="F808B497E5F3DEF3" modified="2011-11-16" version="1">MTMISKGVRYFIGYSAISVAALAWSIYDSLTTSYNFIMFLIEISDGIKLGITINSIILAFILTIKLLQFILFGSLRIIEVEHIFEKLPFFIIGTFLNLATGDNDIILNVLLMGLSIMFKFIHIVMFDRLDFLNLRIVNKLSSGETVTIGTVVRHFAGSSNFWLNIICVVLDFFIAKFLVYDVFQGINSVTCLLFGFQFAVQGVEALTNFAKLMLGIYELIFYRIERGSTVRSETSIETVEPTQETSVETVEPTEESTEESTEEPTEEPTEEPTEEPTEEPIEQPLDEDLFEDDDDFERIWENKTYYSKGIDIGSSILTAVSYLGFIYLLIFESGLNLPLSMIQGTYSAVRQAYVQINQLRVFIESSKRLDTQLANATTEDLNESDNLCIICREDMHSIEDYERLFHKPQPSRRSPKKLLCGHILHMGCLKEWMERSDSCPLCRRKVFGGQPSVPTTTPPQVPVPQPQPGAVPIPPVETGTAAATATATATATATGTGTTTPVENQQAQPQNTTNFFANTQPTPTPTSTATTSHVHTPIINHEEITDRSNEDGIHHQIFLPSNALLPPDWILLPLEKSDGDCVNYKVKLSKFHTADLKVKTKDPNREIIVYDIPQESSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJV8</accession>
    <name>G3AJV8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_135403</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373593.1">
        <property type="nucleotide sequence ID" value="XM_007373531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJV8"/>
    <dbReference type="FunCoup" id="G3AJV8">
        <property type="interactions" value="1296"/>
    </dbReference>
    <dbReference type="GeneID" id="18869887"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135403"/>
    <dbReference type="eggNOG" id="ENOG502QRFX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_42_5_1"/>
    <dbReference type="InParanoid" id="G3AJV8"/>
    <dbReference type="OMA" id="ATIYPIQ"/>
    <dbReference type="OrthoDB" id="10264707at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052742">
        <property type="entry name" value="Mito_N-acetyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015416">
        <property type="entry name" value="Znf_H2C2_histone_UAS-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43138">
        <property type="entry name" value="ACETYLTRANSFERASE, GNAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43138:SF2">
        <property type="entry name" value="PROTEIN SPT10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09337">
        <property type="entry name" value="zf-H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="73"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="513"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="435"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="544" mass="61827" checksum="A6A495F8D308A272" modified="2011-11-16" version="1">MPEFDDVLQTPQYRTTGDSTSSPLAEFNPSVSILDRYTVLLKDGETTATIYPIHSPNELPPGLLAFLCDEFNMEIERGDTLPFFDTLHIEQFQQYWFGSFAAVMVLGDSPSLDGPPRQWEKECLGTFSIKPNYPGRCSHICSAQFLVNAGIRGKGIGRTLTDCFLQWAPRLGYSYCMFNLVFETNVAARKLWESLNFKRIGRIKSAGVLKGHEHPVDAVIYGRDLVSATDPTVGAYRFDKIKFYLETGRYPAMADRSEKSRLRSSASHYNLEDGKLMLKGREVVGDPVRQLQICTDLHMQNHGGINKTTSLVTEKYHWVRIKDTVATAIKNCPECRDPTREIAMVKRGIPKKMSTGQPSATVTRHRINKVYSNQSRNLMRSRAAQDEVEAMVAAAQLHNLPSNRHDDLLSHELAGLDDNLIAVVEEAQRNQQQRMQKERQQQHQHSDQQQNRHVQTYAEVAAAAVANDADYQEYDYHEYSKRNNEDDIPVDPQVSSYDHGNGDEIEIARALIQANEDEHERQSNRKSSRHEEESEANMFIKDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK68</accession>
    <name>G3AK68_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60223</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374393.1">
        <property type="nucleotide sequence ID" value="XM_007374331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK68"/>
    <dbReference type="GeneID" id="18873281"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60223"/>
    <dbReference type="HOGENOM" id="CLU_2962376_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK68"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="59" mass="6097" checksum="63A9A01A48EEA060" modified="2011-11-16" version="1">MGFCKHSSRQPPVPFSSAVLSCPSIHRDSSSSSSSATAASPLRAPRLNGSDTVLTPIIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKA2</accession>
    <name>G3AKA2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_60900</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PGA52 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375076.1">
        <property type="nucleotide sequence ID" value="XM_007375014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKA2"/>
    <dbReference type="FunCoup" id="G3AKA2">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKA2"/>
    <dbReference type="GeneID" id="18873625"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60900"/>
    <dbReference type="eggNOG" id="ENOG502QSTV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030276_4_0_1"/>
    <dbReference type="InParanoid" id="G3AKA2"/>
    <dbReference type="OMA" id="STIHDYQ"/>
    <dbReference type="OrthoDB" id="118256at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018805">
        <property type="entry name" value="YJL171C/Tos1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018807">
        <property type="entry name" value="YJL171C/Tos1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31737:SF3">
        <property type="entry name" value="CELL WALL PROTEIN YJL171C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31737">
        <property type="entry name" value="PROTEIN TOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10287">
        <property type="entry name" value="YJL171C_Tos1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10290">
        <property type="entry name" value="YJL171C_Tos1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442388" description="glucan endo-1,3-beta-D-glucosidase" evidence="8">
        <location>
            <begin position="21"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Cell wall protein YJL171C/Tos1 N-terminal" evidence="10">
        <location>
            <begin position="27"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Cell wall protein YJL171C/Tos1 C-terminal" evidence="9">
        <location>
            <begin position="103"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="391" mass="42485" checksum="C25DBE30968E9A6F" modified="2011-11-16" version="1">MDKMFSLSVITLYLLHTITAASSQVSAIQFNNFGYSGAYTPVVDLANIYDDTCSCAVASKPTTFSGTNTPLNEEVSVHFRGPLQLYNFASYVSQDFTLGDNSGEFTRLSFYDSAEGTADNVTFLTRAGKNSSCLGFGLTYADTDGISKANSPTVLADDTLINSNQEYVIFSNLTCDKSGVNNDCGVYREDIPAYHGFYGTTKMFLFKFSMPNETKSAWDVANYNMPAIWLLNAQIPRTAQYSNDVNCSCWRSGCGEFDVFEVMNVTEYTHLYSTIHDYQGTDDIESGLAGNGYIDRDLNGTMVGGVVFDSTGKVIVWMSDDTTFDETVQAGDLNSWIKGAEKNAVTTTLPTASLAVATETGTGSSTTKKNSGVVVARSSLITVLSSLLWFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKT7</accession>
    <name>G3AKT7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription activator GCR1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_137813</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374506.1">
        <property type="nucleotide sequence ID" value="XM_007374444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKT7"/>
    <dbReference type="STRING" id="619300.G3AKT7"/>
    <dbReference type="GeneID" id="18870078"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137813"/>
    <dbReference type="eggNOG" id="ENOG502QQ7G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069644_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKT7"/>
    <dbReference type="OMA" id="LTWLMNG"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060963">
        <property type="term" value="P:positive regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052146">
        <property type="entry name" value="HOT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022210">
        <property type="entry name" value="TF_GCR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784:SF2">
        <property type="entry name" value="HIGH-OSMOLARITY-INDUCED TRANSCRIPTION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784">
        <property type="entry name" value="PROTEIN MSN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12550">
        <property type="entry name" value="GCR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription activator GCR1-like" evidence="2">
        <location>
            <begin position="203"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="302" mass="34483" checksum="3AEFBFDB26AC3D31" modified="2011-11-16" version="1">MNTSSGNTNSKHNSHGNIVSGTVQAEGVRPVDTIAYLEDKIDRLNEEIGFLKQVVDFQGSKIDKLASIVSQVFEKKSHQHNHQDEVLTTLQGMQDDVVDHDQVNEVQRQVNQLPHNQDIGAGAGPIESNMDPALQQVAVAAAALQQQQQQQQVSQQQQQQQQQQQQQQQQQQQQTQEQPPRKKRKSKKKEARQGAVPKIVVDFLHNPMSVKEIYDEFTKGFKGQIPLNQMDEKYGKQEWRGDSRSKESKRYQRRKKLCDAIDRGTIKYNKTVDEIIAYIEEFRADKSLTWIMNGNLPSDLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL11</accession>
    <name>G3AL11_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Protein URE2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_60372</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Plays an important role in the cellular response to the nitrogen source. URE2 gene plays a major part in the repression of GLN1 and GDH2 genes by glutamine, and is required for the inactivation of glutamine synthetase. URE2 gene product may catalytically inactivate GLN3 in response to an increase in the intracellular concentration of glutamine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374569.1">
        <property type="nucleotide sequence ID" value="XM_007374507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL11"/>
    <dbReference type="FunCoup" id="G3AL11">
        <property type="interactions" value="718"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL11"/>
    <dbReference type="GeneID" id="18873371"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60372"/>
    <dbReference type="eggNOG" id="KOG0867">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_14_1_1"/>
    <dbReference type="InParanoid" id="G3AL11"/>
    <dbReference type="OMA" id="YEPHRID"/>
    <dbReference type="OrthoDB" id="422574at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051219">
        <property type="term" value="F:phosphoprotein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10293">
        <property type="entry name" value="GST_C_Ure2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03048">
        <property type="entry name" value="GST_N_Ure2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000222">
        <property type="entry name" value="Protein URE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000034">
        <property type="entry name" value="Transcriptional regulator URE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017298">
        <property type="entry name" value="Ure2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF3">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR URE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037861">
        <property type="entry name" value="Prion_URE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 6" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="8">
        <location>
            <begin position="70"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="9">
        <location>
            <begin position="163"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="30"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00058785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR037861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="312" mass="35498" checksum="BCACE77F8E4907DA" modified="2011-11-16" version="1">MSDQNLEQPTNGSNTHTISNLSAGLKSVTLNDQQQNEVNLNLLQQQLQNETTNAQQQSRITKFFQNQPTEGYTLFSHRSAPNGFKVAIILSELNLPFNTIFLDFNHGEQRAPEFVTINPNARVPALIDHFNENTSIWESGAIILYLVSKYLKENGECALWSDNLVEQSQISSWLFFQTSGHAPMIGQALHFRYFHSCPVPSAVERYTDEVRRVYGVVEMALAERREALIMDLDVENAAAYSAGTTPLSQSRYFDYPVWLVGDRATVADLSFVPWNNVVDRIGINLKVEFPEVYKWTKYMMRRPAVIRALRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL66</accession>
    <name>G3AL66_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60421</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374624.1">
        <property type="nucleotide sequence ID" value="XM_007374562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL66"/>
    <dbReference type="GeneID" id="18873397"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60421"/>
    <dbReference type="HOGENOM" id="CLU_2361066_0_0_1"/>
    <dbReference type="InParanoid" id="G3AL66"/>
    <dbReference type="OrthoDB" id="10351482at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="96" mass="10841" checksum="64C472BED282C74B" modified="2011-11-16" version="1">MLRIKPVQTFSRSFSTTPTRHIATTINNGIKGFKPFTSASQVITNYKRGVVSFVTWTVAFYAVMFWPVFPRVISDFINDVPPNLTGINSPDRSLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL75</accession>
    <name>G3AL75_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">60S ribosomal protein L11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60431</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374633.1">
        <property type="nucleotide sequence ID" value="XM_007374571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL75"/>
    <dbReference type="FunCoup" id="G3AL75">
        <property type="interactions" value="1040"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL75"/>
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    <dbReference type="eggNOG" id="KOG0397">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061015_3_0_1"/>
    <dbReference type="InParanoid" id="G3AL75"/>
    <dbReference type="OMA" id="NPMKELK"/>
    <dbReference type="OrthoDB" id="1734943at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1440.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L11"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_uL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_uL5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020929">
        <property type="entry name" value="Ribosomal_uL5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057266">
        <property type="entry name" value="Ribosomal_uL5_euk/arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_uL5_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003258">
        <property type="entry name" value="PRK04219.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00358">
        <property type="entry name" value="RIBOSOMAL_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL5 N-terminal" evidence="6">
        <location>
            <begin position="7"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL5 C-terminal" evidence="7">
        <location>
            <begin position="64"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="19819" checksum="E97A77FE429F2F62" modified="2011-11-16" version="1">MSAKSQNVMRELRIEKLVLNICVGESGDRLTRASKVLEQLSGQTPVQSKARYTVRTFGIRRNEKIAVHVTIRGPKAEEILERGLKVKEYQLKTKNFSATGNFGFGIDEHIDLGIKYDPSIGIYGMDFYVVMGRAGARIARRKRTRGVIGNSHKTNKEDTIQWFKQRYDAEVLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL90</accession>
    <name>G3AL90_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="15">P-type ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_135632</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374648.1">
        <property type="nucleotide sequence ID" value="XM_007374586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL90"/>
    <dbReference type="FunCoup" id="G3AL90">
        <property type="interactions" value="1147"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL90"/>
    <dbReference type="GeneID" id="18869907"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135632"/>
    <dbReference type="eggNOG" id="KOG0209">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001828_4_1_1"/>
    <dbReference type="InParanoid" id="G3AL90"/>
    <dbReference type="OMA" id="QKTKYVW"/>
    <dbReference type="OrthoDB" id="48943at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140567">
        <property type="term" value="F:membrane protein dislocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015662">
        <property type="term" value="F:P-type ion transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140569">
        <property type="term" value="P:extraction of mislocalized protein from ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034214">
        <property type="term" value="P:protein hexamerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055092">
        <property type="term" value="P:sterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07543">
        <property type="entry name" value="P-type_ATPase_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000049">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000071">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057255">
        <property type="entry name" value="2TM_P5A-ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047820">
        <property type="entry name" value="P5A-type_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF7">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE HELIX TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23143">
        <property type="entry name" value="2TM_P5A-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01229">
        <property type="entry name" value="COF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="195"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1009"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1034"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1071"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1151"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1190"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="domain" description="P5A-ATPase transmembrane helical hairpin" evidence="14">
        <location>
            <begin position="19"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="13">
        <location>
            <begin position="251"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW33133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1241" mass="138881" checksum="BE2693EA7EC8F67C" modified="2011-11-16" version="1">MSNLVATPNIQDAELLVPKSFFLRPYVWPFTIIYPICFQIYFQHYDTYIVGREWTFVYSIAIISLNLLFWLMPHWNIDIDAKFNYSKVNNVAEASYIKVTPAPNSGTDEICKIHRETFHDGEKQTSFLYQKRRYLFHTELSKFSPPEFVFDELPKLSVFQNTKGLSGDLEKQVRNYGQNKFDIPIPTFMELFKEHAVAPFFVFQIFCVALWCMDEQWYYSLFSLFMLVSFEMTTVFQRRTTMSEFQSMAIKPYKVYVYRDAKWVELETTELLPGDVISVTRTSEESALPCDLLLIDGSAIVNEAMLSGESTPLLKESIKLRPSDDNLQPEGFDKNSILHGGTMALQVTKPENSVIPVAPDNGALAVVTKTGFETSQGSLVRMMIFSSERVSVGNKEAFFFILFLLIFAIAASWYVWVEGTRMGRVQSKLILDCIIVITSVVPPELPMELTMAVNSSLTKLQKCFVYCTEPFRIPLAGRIDVCCFDKTGTLTAEDLVFEGLAGFNVNDINHLYKCDEAPETTSYVLGSAHALVRLDDGEVVGDPMEQATLKAANWKVGGNDTVERDYGKNSAAKSKASKGEKIKILRRFQFSSALKRSSTISQVSAISGKNFVATKGAPETIRTMIVDAPENYEDIYKSFTRAGSRVLALAYKYLDTSVNVNKVKREDIESGLKFAGFIVFHCPLKNDAIETIRMLNASSHRSVMITGDNPLTACHVAKEVRITTKDVLILDAPEEHHEIGEDDNLVWRNVDESVIIPYMSSDPINVELFAKYDICITGHALMHLSEHAQILELLKHTWVYARVSPNQKEFIITSLKDAGYNTLMCGDGTNDVGALKQAHIGVALLNGTEEGMKKIAENRKMEMSARVYDKQVALFTQWNRPPPPVPALIAHLYPPGPLNPKYLEAMEKKGVEITPEMRKAVEIANKQPYKPPKKTDLAVAESKEKGGFADTLLGALNDAELDDETPALKLGDASVAAPFTSKLSNVSAVTHIIRQGRCALVSTIQMYKILALNCLISAYSLSVLYLAGIKFGDGQATISGILLSVCFLSISRGRPLEKLSKERPQDGIFNIYIMGSILGQFAIHIITLVYITREIYILEPREPQVDLEKEFSPSLLNTGMFLLQLAQQVSTFAVNYIGLPFRESIKDNKGMYYGLLGVAGLAIAGSTEFIPELNEAMQFVPMSQEFKVKLTGSILVDLGVTYLIEVTLKHFFMNSAAADIALREDDEDAGYFDDKKVIDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM56</accession>
    <name>G3AM56_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Oligomycin resistance ATP-dependent permease YOR1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_54783</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374276.1">
        <property type="nucleotide sequence ID" value="XM_007374214.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AM56">
        <property type="interactions" value="275"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM56"/>
    <dbReference type="GeneID" id="18871887"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54783"/>
    <dbReference type="eggNOG" id="KOG0054">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_27_1_1"/>
    <dbReference type="InParanoid" id="G3AM56"/>
    <dbReference type="OMA" id="QVTDAWT"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18597">
        <property type="entry name" value="ABC_6TM_YOR1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18606">
        <property type="entry name" value="ABC_6TM_YOR1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001750">
        <property type="entry name" value="ATP-binding cassette transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000010">
        <property type="entry name" value="Multidrug resistance-associated ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="258"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="288"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="366"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="413"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="841"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="881"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="958"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1070"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1094"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="145"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="520"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="846"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="1161"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="481"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="749"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="483"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="493"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="752"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="787"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW32761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1426" mass="161412" checksum="47D0E185C533B544" modified="2011-11-16" version="1">MSEDPHFLQQKRLLTFMLSKKVPPIPTEEERDPYPVKKANIISRILFWWLGPVMHTGYRRTLQPEDLFYLTDDIKVQTMADRFYNYMTNDIERARQQHIAEKCKERGETPETSSVDRGKDLADFELSKFLTVWALAKTYKWQYTWACTLLCLSSVGQTTLPLLTKKLIRYVELKSMGVETGIGRGIGYSFGSAIIIFIIGVLINHFFYRSMLTGAQAKAVLTKALLDKSFKLNAAARHKYPVGKITSMLGTDLSRIDFALGFQPFLICFPVPIGIAIGILIWNIGVAALVGVAILLVFMVCIAVSTGALFKYRKKANKYTDSRVDHIKEALNNLKIIKFYSWEPPYHENISEIRKKEMKIIYRMQVLRNVVTSFAFSLTLFASMTAFLVLYAIAANRKDPASIFSSLSLYNILTQQVFLLPMALATGADAFMGISRVGEFMSQGEIDPALSNIDATPEKKLLMENDETAIEVDHASFEWEVFGNDEDDEEESESKKGEKKSMEKKVHKTEVHYHEKTGSIEKDSLTTSSSGRGEEESQFPGLKDINFKIKKGEFVVITGLIGSGKTSLLNAISGFMKRVHGDVSTNGSLLLCGYPWVQNSTVKENILFGEPYDEKKYKQVIYACSLEADLEILPAGDRTEIGERGITLSGGQKARINLARAVYANRDIILLDDVLSAVDARVGKHIMNNCIMDLLKDKTRILATHQLSLIGSADRVIFLNGDGSVDVGTFEELSSSNPGFSKLMTFNSEAHNDEEEEEDVPESEDELEQEREMIKRQLTRLSTRASTKADPEDEEARHREFNTDESADGKLIDEEERAVNAISMRVYGRYIELGSGAVGPYVYGPLLLIFLMFATFCSIFTNTWLSFWVERRFPLEDKVYIGVYIMFTFLAFIFLTIEFILLVYLTNTASVKLNILAMKKVLHAPMSFMDTTPLGRILNRFTKDTDVLDNEIGDQLRFFLFTLSNIIGVLILCIIYLPWFAISIPFLGFLFVAIANYYQASAREIKRLEAIQRSFVYNNFNETLSGMTTIKAYHAVPRFLEKNNFLIDRMNEAYYLTIANQRWLAIHMDMVASLFALLIALLCVNRVFRISAASVGLIVAYVFQIAGQLSMLIRTFTQVENEMNSVERLDSYASNLPEEAPYVITEKTPPPQWPDKGSIEFRSASLAYRPGLPLVLKNLNFTIKPSEKIGICGRTGAGKSSIMTALYRLSELESGKIFIDDLDIAELGLKDLRSKLSIIPQDPVLFRGTIRKNLDPFNQSSDDKLWDALRRTGLIEEGRLEQVKLTNKPSDGSSETNLHKFHLDQSVEDEGTNFSLGERQLIAFARALVRDSKILILDEATSSVDYETDSKIQHTIIREFSHCTILCIAHRLKTIINYDRILVLDKGEIREFDTPWNLFKSNGSIFQQMCQRSNITDQDFENITSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM77</accession>
    <name>G3AM77_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Replication factor C subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_71228</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the activator 1 large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374890.1">
        <property type="nucleotide sequence ID" value="XM_007374828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM77"/>
    <dbReference type="FunCoup" id="G3AM77">
        <property type="interactions" value="1044"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM77"/>
    <dbReference type="GeneID" id="18875368"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_71228"/>
    <dbReference type="eggNOG" id="KOG1968">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003574_1_0_1"/>
    <dbReference type="InParanoid" id="G3AM77"/>
    <dbReference type="OMA" id="LICNERN"/>
    <dbReference type="OrthoDB" id="446168at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902983">
        <property type="term" value="P:DNA strand elongation involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000005">
        <property type="entry name" value="Replication factor C subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000395">
        <property type="entry name" value="Replication factor C subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013725">
        <property type="entry name" value="DNA_replication_fac_RFC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012178">
        <property type="entry name" value="RFC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF6">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25361">
        <property type="entry name" value="AAA_lid_RFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08519">
        <property type="entry name" value="RFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036578">
        <property type="entry name" value="RFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="10">
        <location>
            <begin position="182"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="256"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="824"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="267"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="824"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="843"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="866"/>
            <end position="878"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR036578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="878" mass="96997" checksum="F1AE2DB4D7019549" modified="2011-11-16" version="1">MVNITDFFSGKAKPSQKRKASASPEPETKKPKASTNKRAPKQEPEVIDLDDDDDFSFSDEGDDSLVEIKPATPKRKPAAPKVATPKKATPKKATPQKATPKKEPASKKPSSPKKAMAKPAASSASTVTLSAEEVLATIPDAVLPEIDDSKKVNFFALNAAKQANANAAGSGTIDIPEAQPNFFTGQLPNLDRDSAESLAKRYGAKVTKSISGKTSLVVLGDDAGPSKVKKIKQLHIKTIDEDGFVELLKRMPLEGGSGEAAQKAKLKREEEERKVIEQAEHDARVEAEQEEKQRKKLAAAQATASKSSQGSSISAPPLQPIIPAKDKLWTDKHAPTSTSQLCGNKGQIAKLRNWLDNWFENQKHGFTKAGKDGSGVYRAVLISGPPGIGKTTAAHLVANELGYDVLEKNASDVRSKSLLNATVKSILDNTSVVGFFKQREDKQLGDNSKKICLIMDEVDGMSSGDHGGAGALSQFCRITHMPMILICNDKSLPKMRTFDRVCFDLPFRRPSEQEVKSRLMTIALREGVKLDPNIIGQLVQATSNDIRQMINLMSQVSKTQKAIGNATLEEINQGWKKQVILKPFDIAGRLLSSGIWTSPRQNLNEKLDLYFNDIDFAPLMIQENYLLTRPRLPGKHLDHIARAADDISQSDLINSLIRSSEQQWSLLPFHGLMSSVRPSYEVAGMVQGRLAFSSWLGQNSKQMKYQRILQELQYHTRIKTSTTKQELRLDYLNPLWNKLVEHIHDEDGITKTMKVMDEYFLTKEDFDNIAEILKKDAGISPKMKTSFTKAYNKAIHPTVIYKTGNSVLNGGKRGAASKVDYEDVVDDDMEDVPDEEPENSDTIDNKRDKLIKVEKPKARGKAKTKAAKDEPAKKKQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM92</accession>
    <name>G3AM92_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_136980</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TACO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374906.1">
        <property type="nucleotide sequence ID" value="XM_007374844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM92"/>
    <dbReference type="FunCoup" id="G3AM92">
        <property type="interactions" value="322"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM92"/>
    <dbReference type="GeneID" id="18870007"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136980"/>
    <dbReference type="eggNOG" id="KOG2972">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062974_1_0_1"/>
    <dbReference type="InParanoid" id="G3AM92"/>
    <dbReference type="OMA" id="FGPGGCM"/>
    <dbReference type="OrthoDB" id="2017544at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099617">
        <property type="term" value="C:matrix side of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.200:FF:000002">
        <property type="entry name" value="Probable transcriptional regulatory protein CLM62_37755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.980">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00693">
        <property type="entry name" value="Transcrip_reg_TACO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017856">
        <property type="entry name" value="Integrase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048300">
        <property type="entry name" value="TACO1_YebC-like_2nd/3rd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049083">
        <property type="entry name" value="TACO1_YebC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002876">
        <property type="entry name" value="Transcrip_reg_TACO1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026564">
        <property type="entry name" value="Transcrip_reg_TACO1-like_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029072">
        <property type="entry name" value="YebC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12532">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR OF CYTOCHROME C OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12532:SF0">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR OF CYTOCHROME C OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20772">
        <property type="entry name" value="TACO1_YebC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01709">
        <property type="entry name" value="Transcrip_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75625">
        <property type="entry name" value="YebC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TACO1/YebC-like N-terminal" evidence="4">
        <location>
            <begin position="27"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="TACO1/YebC-like second and third" evidence="3">
        <location>
            <begin position="105"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW33391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="277" mass="31031" checksum="8B208E909A3B546C" modified="2011-11-16" version="1">MFRALTPIQTCRTLCLPLRQVRWAGHSKWANIKHDKAKNDAKKSKEAYHLSHRIESAVRQGGKENNPTLDMFVEKAKKLNVSKAVIEKAIKRGAGEMVSDGPALSDVVYELMGPGGVAFVVQASTDNKSRTVSQVRNALAEFSATASPCMYMFEKKGEVVFLPKADANESFDDVFEVALDIGAEDVEEVEIDNIKMPLVYRLLCDQTEINNIVKELGSRGYQIQNSSIRYIANSDSRVPYPENNKGFDKAIDALDDVRDVVDYYTNIEDEHLHRLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMM4</accession>
    <name>G3AMM4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 17">Delta-aminolevulinic acid dehydratase</fullName>
            <ecNumber evidence="5 17">4.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_60819</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 17">
            <text>2 5-aminolevulinate = porphobilinogen + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 17">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the ALAD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.24" evidence="5 17"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374983.1">
        <property type="nucleotide sequence ID" value="XM_007374921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMM4"/>
    <dbReference type="FunCoup" id="G3AMM4">
        <property type="interactions" value="639"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMM4"/>
    <dbReference type="GeneID" id="18873587"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60819"/>
    <dbReference type="eggNOG" id="KOG2794">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035731_0_1_1"/>
    <dbReference type="InParanoid" id="G3AMM4"/>
    <dbReference type="OMA" id="YQMDYAN"/>
    <dbReference type="OrthoDB" id="1530at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000048">
        <property type="entry name" value="Delta-aminolevulinic acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030656">
        <property type="entry name" value="ALAD_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006762">
        <property type="entry name" value="PRK09283.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458:SF0">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00169">
        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="10 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 17" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="14">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="14">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW33468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="341" mass="37446" checksum="D42CCD893EC1C38C" modified="2011-11-16" version="1">MVHTAEFLPSSGSSISSILQGGYHHPLAREWQQERQLTKNMFIFPLFVTDRPDEESDIPSLPNIKRRGVNKLIPYVKSLVEKGLRSVILFGVPLKEGVKDQVGTAADDPEGPVIQSIKLLRKNFPDLFIMCDVCLCEYTDHGHCGLLNEDGTLNREPSVQRIAAVAVNYAKAGAHSVAPSDMMDGRIKDIKLGLINAGLGNKCFVMSYAAKFSGNLYGPFRDAAGSTPGQGDRKAYQLPPGGAGLARRALVRDMEEGADAVIIKPSTFYLDIVSDASRLCRDYPICTYQVSGEYAMLHAAAEKGVFDLKAIAFESHQGYLRAGARLIISYFTPEFLEWLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMP5</accession>
    <name>G3AMP5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial inner membrane i-AAA protease complex subunit MGR1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_60835</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MGR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375003.1">
        <property type="nucleotide sequence ID" value="XM_007374941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMP5"/>
    <dbReference type="FunCoup" id="G3AMP5">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="GeneID" id="18873595"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60835"/>
    <dbReference type="eggNOG" id="ENOG502QR67">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_871878_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMP5"/>
    <dbReference type="OMA" id="EFEMVWL"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013911">
        <property type="entry name" value="i-AAA_Mgr1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08602">
        <property type="entry name" value="Mgr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="356"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="365" mass="40418" checksum="7481D76D6A93FA34" modified="2011-11-16" version="1">MGVYVPPPDDNDPKKNQNQKNSNSSPASGSNSSSSPSGSTTIIIPNPVALIPRNPSVGLIWGPLMPASDNRPALYASIGAQFLLGLAMFRHARGLFRLQPIATGLNTPPVMGRRGPLWRVLLSVVTGSGLVFGSGLELSRMTLPYDPWYEEAQHYRKVATKQGDKPSAWFGAYKYYRPMDLNTWINKVGDWIKNVEKEIEDPNHMSNVTLELGKNSNVIVNKPVAGGILSKLNNKGKYQEIYNKLQESNVNRRKQLLESELKDVVELNKADRLDAILEGTSDLNDPDYSKPSIQLGNHSLETDDDLEMVWLNFEPWDELKLETDYDIRLIPRCSVAGKHSELVEATATTRGVPSSGDEENTLTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMP7</accession>
    <name>G3AMP7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60843</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375006.1">
        <property type="nucleotide sequence ID" value="XM_007374944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMP7"/>
    <dbReference type="GeneID" id="18873597"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60843"/>
    <dbReference type="eggNOG" id="ENOG502RQAJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1171244_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMP7"/>
    <dbReference type="OrthoDB" id="4096888at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="237" mass="26490" checksum="1CD7019FE98DE0B3" modified="2011-11-16" version="1">MQQENLQLKSNIYANKVNNPTFIPSSGLLEENSKSAIKISDKAIKKSKAKKDASGVRLNYKIEAKYEEEEQSLSQKNIKIKLPNIFRDTPESDYFLKDMISSQEANRYGEDYCSTFYKRNIHGYMFIKEPSNSLKVNASGGKSSVSLKISLPAGNNRLLAKKLKVDVKELPIWKPISSGNGGTASFTARRRPNVNSNNTSNGTKETKGIQATQDPPKLKNGNLSKRFGRRNTPKQTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMR7</accession>
    <name>G3AMR7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60857</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375026.1">
        <property type="nucleotide sequence ID" value="XM_007374964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMR7"/>
    <dbReference type="STRING" id="619300.G3AMR7"/>
    <dbReference type="GeneID" id="18873603"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60857"/>
    <dbReference type="eggNOG" id="ENOG502QS34">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046574_1_0_1"/>
    <dbReference type="InParanoid" id="G3AMR7"/>
    <dbReference type="OMA" id="VFPMTWK"/>
    <dbReference type="OrthoDB" id="93019at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051178">
        <property type="entry name" value="TfdA_dioxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43779:SF2">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT XANTHINE DIOXYGENASE XAN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43779">
        <property type="entry name" value="DIOXYGENASE RV0097-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="7">
        <location>
            <begin position="136"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="393" mass="44257" checksum="06D744047A4F2F3C" modified="2011-11-16" version="1">MHKDSLEIHPLDFHFGCSITLPPYAKNDPANLNEQDFKKLYEAVHQYLVVVIPGQEKLSPKSQYLLTKRFDPSIPEPKDTAGANYGHGKEFRHTQSVLRKDGTSVKSQPQVQILGQGVFDKDEPGNENGEAIKLTHPSHTTFHHTPLTPDQIKNESKTRFYRWHIDSALYDLSPPMVTTLLGIKVPPTTQYQTISYDDGTGDELKLTQGATCFVSGAQAFKNLSDEDQEFALNTTVEYAPHPYIFISPARATSDGLTMVSEGKEMNFEELPEWEASKVKKLPMVWTNPVTKEHHLQVHGCCLYKLHTKVGETIETLALEDAREKVHKLMRPNISPNQILAHGWKQGDLVIFYNRGVWHSVTGEFKGVGSNGEDERRLMHQCNIASGIDPVTIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMZ7</accession>
    <name>G3AMZ7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein SVP26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_139419</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SVP26 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375687.1">
        <property type="nucleotide sequence ID" value="XM_007375625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMZ7"/>
    <dbReference type="FunCoup" id="G3AMZ7">
        <property type="interactions" value="263"/>
    </dbReference>
    <dbReference type="GeneID" id="18870208"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139419"/>
    <dbReference type="eggNOG" id="KOG4136">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058268_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMZ7"/>
    <dbReference type="OMA" id="TMGTEPV"/>
    <dbReference type="OrthoDB" id="28257at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097020">
        <property type="term" value="F:COPII receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007277">
        <property type="entry name" value="Svp26/Tex261"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13144:SF0">
        <property type="entry name" value="PROTEIN TEX261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13144">
        <property type="entry name" value="TEX261 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04148">
        <property type="entry name" value="Erv26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="25244" checksum="0D7B4F7667B87F9E" modified="2011-11-16" version="1">MILKLLSYLGTVVGFLFLVLSIASGLYYISELVEEHTEPTKRFLTKLIYFIIAVFVLLIIFDGFPIKLTLLSIFSYYVYLQNLKRFPYVELTSPIFLISCVLVVANHFLWFNHFHTPYIPPLEVRLKPNYKPPHIPEFVEVCSFFGLLVWFVPFALFVSLSAHDNLLPHHVESTQDTERKKKNGLAKVVVSRIRDYIYAISRKLGYELDPHHGTIVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AND4</accession>
    <name>G3AND4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GIY-YIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_50535</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalytic subunit of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Forms a heterodimer with SLX4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the SLX1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375199.1">
        <property type="nucleotide sequence ID" value="XM_007375137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AND4"/>
    <dbReference type="FunCoup" id="G3AND4">
        <property type="interactions" value="418"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AND4"/>
    <dbReference type="GeneID" id="18871453"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50535"/>
    <dbReference type="eggNOG" id="KOG3005">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030739_1_1_1"/>
    <dbReference type="InParanoid" id="G3AND4"/>
    <dbReference type="OMA" id="INPREER"/>
    <dbReference type="OrthoDB" id="24645at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033557">
        <property type="term" value="C:Slx1-Slx4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10455">
        <property type="entry name" value="GIY-YIG_SLX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03100">
        <property type="entry name" value="Endonuc_su_Slx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027520">
        <property type="entry name" value="Slx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050381">
        <property type="entry name" value="SLX1_endonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20208">
        <property type="entry name" value="STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20208:SF10">
        <property type="entry name" value="STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2 8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIY-YIG" evidence="9">
        <location>
            <begin position="8"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="292" mass="33783" checksum="DF460295EBB72553" modified="2011-11-16" version="1">MSLHTTPQFYCVYLLQSIPKPRTFYVGSTPDMKRRLRQHNGDLKAGGAFRTKRKGSRPWKVVTIVYNFPSRISALQFEHSLQHPQFTRRISHDDRVSKSQSTSLHQRLANIKLLITSPGFVRMDLKVKIFDDEVYLAWRTDKYDVKFDREVELGKFDELDINECDDDFDAMKQKALDEDISCCICKANIDVFKDVPEITSRHEFAEFLGRFPLVSLCYNTNSTFHLTCMARSQDQLIPTSVNCPCCSKSLTWSRIVKTSTKLRYYLLKDNVKGLTQPTQDACTQELLTQNTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANK2</accession>
    <name>G3ANK2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Alanyl-tRNA synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_138667</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II aminoacyl-tRNA synthetase family. Alax-L subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375808.1">
        <property type="nucleotide sequence ID" value="XM_007375746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANK2"/>
    <dbReference type="FunCoup" id="G3ANK2">
        <property type="interactions" value="254"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANK2"/>
    <dbReference type="GeneID" id="18870139"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138667"/>
    <dbReference type="eggNOG" id="KOG2105">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004485_7_1_1"/>
    <dbReference type="InParanoid" id="G3ANK2"/>
    <dbReference type="OMA" id="KYDTTSW"/>
    <dbReference type="OrthoDB" id="288942at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043905">
        <property type="term" value="F:L-seryl-tRNA(Thr) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002196">
        <property type="term" value="F:Ser-tRNA(Ala) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000012">
        <property type="entry name" value="Threonyl/alanyl tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051335">
        <property type="entry name" value="Alanyl-tRNA_Editing_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43462">
        <property type="entry name" value="ALANYL-TRNA EDITING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43462:SF1">
        <property type="entry name" value="ALANYL-TRNA EDITING PROTEIN AARSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alanyl-transfer RNA synthetases family profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="287"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="443" mass="49161" checksum="C9867235AE8250D3" modified="2011-11-16" version="1">MTTAIVGALACQKNSFLKVFNTTVVSSFEFVPPPTPRDKQNKNKKSSSPEVKQTEYGVELKDTILFPEGGGQPFDKGTITLPDNKEITVKSVIRDKLKAIHITNELIEPGTEVTLKVDWNRRIDIMQQHTGQHLLSAVFDTYNLETLSWSMGDVINYIELPEKVSEEIVAEVNTKVNELILENHEIKVVTPDQHGHEVDVSHLPDDYDLSKGIIRIVKIGELDANPCCGTHLTSTGQIQSIALLHQVSVRGGHSRLYFTCGNRVSNYLRKEHEILKDVSGSQLSCQIEEVVDKVAQLNKNYRKANSTVSGLLKEIAGMEAARLFTKFQKDEQSVGYVYRADNNPEYLTSVQKEISTLINNSKGLNVDLSSKQTLVLINGDYPSGTGGMVKVMGPKAEEIQSELKQRLTQLKGGGKGASFQGKIPKYEKGELESVLLYLNTICD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANM5</accession>
    <name>G3ANM5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61619</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375830.1">
        <property type="nucleotide sequence ID" value="XM_007375768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANM5"/>
    <dbReference type="GeneID" id="18873978"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61619"/>
    <dbReference type="HOGENOM" id="CLU_1682185_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANM5"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="157" mass="17155" checksum="34B8C354E9A48815" modified="2011-11-16" version="1" fragment="single">MEEYNHTRPRQNSVSVSPYSNGSTPQVQTPQVQASQSYPQLTKIHQGYAPQYQQQFQQTNQFSNTTIVASEVPPPRPGAVVGPTQIPAGTPIEAQTATMQQPYHQWPPTLPPTRSNSTPNNIHLPPIPLNPSHPNLPPPPVIIPGASVPGRRTNHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANP8</accession>
    <name>G3ANP8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein transport protein SFT2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_55556</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Nonessential protein required for the fusion of transport vesicles derived from the endocytic pathway with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Golgi apparatus membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the SFT2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375259.1">
        <property type="nucleotide sequence ID" value="XM_007375197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANP8"/>
    <dbReference type="STRING" id="619300.G3ANP8"/>
    <dbReference type="GeneID" id="18871948"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55556"/>
    <dbReference type="eggNOG" id="KOG2887">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099529_3_0_1"/>
    <dbReference type="InParanoid" id="G3ANP8"/>
    <dbReference type="OMA" id="PTHNEGP"/>
    <dbReference type="OrthoDB" id="660759at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011691">
        <property type="entry name" value="Vesicle_transpt_SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137:SF36">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SFT2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="210" mass="23688" checksum="1776B5958846E34D" modified="2011-11-16" version="1">MSFFKSFTRSNEPNYSRLGQNSDTLPTPVTQSDRPSSFKSTLNRLNPFSDNSIQLQDEEIVTSEPGYFELSRWDRMLIFALTFGGSCCCYLICIFLFPILSLKPRKFALLWSLGSIFFLISFGVLQGFKPYMEHLFSSTRLIFTVVFVSSIILTLISSVSLKSTLLSIIFAVIQLGAALWYTVSYFPMGRQTLNLAGTVARGQVDNWLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANV6</accession>
    <name>G3ANV6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 14">6-phosphogluconate dehydrogenase, decarboxylating</fullName>
            <ecNumber evidence="10 14">1.1.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">GND1</name>
        <name evidence="16" type="ORF">SPAPADRAFT_51124</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10 14">
            <text>6-phospho-D-gluconate + NADP(+) = D-ribulose 5-phosphate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:10116"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="1.1.1.44"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10 14">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10 14">Belongs to the 6-phosphogluconate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.44" evidence="10 14"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375857.1">
        <property type="nucleotide sequence ID" value="XM_007375795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANV6"/>
    <dbReference type="FunCoup" id="G3ANV6">
        <property type="interactions" value="970"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANV6"/>
    <dbReference type="GeneID" id="18871521"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51124"/>
    <dbReference type="eggNOG" id="KOG2653">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024540_4_2_1"/>
    <dbReference type="InParanoid" id="G3ANV6"/>
    <dbReference type="OMA" id="CVTHVGP"/>
    <dbReference type="OrthoDB" id="434986at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00410"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004616">
        <property type="term" value="F:phosphogluconate dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019521">
        <property type="term" value="P:D-gluconate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000002">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.320:FF:000002">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000007">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.320">
        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006114">
        <property type="entry name" value="6PGDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006113">
        <property type="entry name" value="6PGDH_Gnd/GntZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006184">
        <property type="entry name" value="6PGdom_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006183">
        <property type="entry name" value="Pgluconate_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00873">
        <property type="entry name" value="gnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006765">
        <property type="entry name" value="PRK09287.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11811">
        <property type="entry name" value="6-PHOSPHOGLUCONATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00393">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000109">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00076">
        <property type="entry name" value="6PGDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01350">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00461">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0311">Gluconate utilization</keyword>
    <keyword evidence="5 10 14" id="KW-0521">NADP</keyword>
    <keyword evidence="6 10 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 10 14" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase C-terminal" evidence="15">
        <location>
            <begin position="198"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="29"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="52"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="94"/>
            <end position="96"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="122"/>
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        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
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    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <begin position="148"/>
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            <position position="210"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00002526"/>
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            <dbReference type="ARBA" id="ARBA00004874"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00008419"/>
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            <dbReference type="ARBA" id="ARBA00048640"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW32581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="512" mass="56075" checksum="FD5E473790A59EB7" modified="2011-11-16" version="1">MSAISRLSVISRHISNTNKLPRGDLGLIGLAVMGQNLILNAADHGYTVVAYNRTTSKVDHFLANEAKGKSIIGAHSIKELVDNLKRPRRVMLLVKAGKPVDDFIDQLLPYLEEGDIIIDGGNSHFPDTNRRYDELKGKGILFVGSGVSGGEEGARTGPSLMPGGHPEAWPHIKDIFQDIAAKSDGEPCCDWVGEAGAGHYVKMVHNGIEYGDMQLICEAYDLMKRVGKFTDKEIGDVFTQWNKGVLDSFLIEITKDILYYNDPTDGKPLVEKILDTAGQKGTGKWTAVNALDLGMPVTLIGEAVFSRCLSALKEERVAASKVLVGPTVEDGASPITDRKKFVDDLEQALYASKIISYAQGFMLMNEAAKEYGWKLNNAGIALMWRGGCIIRSVFLGEITAAYRENPELENLLFHPFFNKAITKAQSGWRASIAKAVEYGVPAPAFSTALAFYDGLRSARLPANLLQAQRDYFGAHTFQVLPGQENDLLKKGEWIHINWTGRGGNVHATTYDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP00</accession>
    <name>G3AP00_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Carbon catabolite-derepressing protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_61130</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Essential for release from glucose repression. It interacts and has functional relationship to the regulatory protein SNF4. Could phosphorylate CAT8.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375307.1">
        <property type="nucleotide sequence ID" value="XM_007375245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP00"/>
    <dbReference type="FunCoup" id="G3AP00">
        <property type="interactions" value="1054"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP00"/>
    <dbReference type="GeneID" id="18873731"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61130"/>
    <dbReference type="eggNOG" id="KOG0583">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_59_3_1"/>
    <dbReference type="InParanoid" id="G3AP00"/>
    <dbReference type="OMA" id="SKTKWHF"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000144">
        <property type="term" value="C:cellular bud neck septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005641">
        <property type="term" value="C:nuclear envelope lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004679">
        <property type="term" value="F:AMP-activated protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061762">
        <property type="term" value="P:CAMKK-AMPK signaling cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071940">
        <property type="term" value="P:fungal-type cell wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010920">
        <property type="term" value="P:negative regulation of inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900436">
        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms in response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045722">
        <property type="term" value="P:positive regulation of gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000222">
        <property type="term" value="P:positive regulation of pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000217">
        <property type="term" value="P:regulation of invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090606">
        <property type="term" value="P:single-species surface biofilm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032933">
        <property type="term" value="P:SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12122">
        <property type="entry name" value="AMPKA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14079">
        <property type="entry name" value="STKc_AMPK_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14334">
        <property type="entry name" value="UBA_SNF1_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000544">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000236">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032270">
        <property type="entry name" value="AMPK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013896">
        <property type="entry name" value="SNF1_UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16579">
        <property type="entry name" value="AdenylateSensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08587">
        <property type="entry name" value="UBA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="51"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="402"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW32031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="601" mass="68536" checksum="1D0127C6938A7D04" modified="2011-11-16" version="1">MSDQVPMDQTGQDDRQQQQHHHHHHHHHQDKQSQQVPVDPSGNPANRIGKYQIIKTLGEGSFGKVKLAHHLVTGQRVALKIINRKTLAKSDMQGRVEREISYLRLLRHPHIIKLYDVIKSKDEIIMVIEYAGKELFDYIVQRGKMPQDEARRFFQQIIAAVEYCHRHKIVHRDLKPENLLLDDQLNVKIADFGLSNIMTDGNFLKTSCGSPNYAAPEVISGKLYAGPEVDVWSAGVILYVMLCGRLPFDDEFIPALFKKISNGVYTLPNYLSPGAKHLLTRMLVVNPLNRITIHEIMEDEWFKQDIADYLLPPDLSKNKHKKIDIDEDVIKALSMTMGYDRDEIINVIERCNKSNAPQQQSNEILDAYLLMKENHSLVKDLKQSKQDKIDSFLSQSPPPTSFTPGSTSSAPGVQQSLTYQTLATVPDLSQLPTSTIAILPSSLPSIHRAFMMELNPNQLSSNINPTPTKKSKTRWHFGIRSRSYPLDVMGEIYRALKNLGAEWAKPTEEELWTIRVRWKYESGDAMQENTPNLMKMQIQLFQLEPNNYLVDFKFDGWETSTGTPQDSFKQDLDEVGSFSAYPFLHLATRLIMELAVNSQSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3APA5</accession>
    <name>G3APA5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphotransferase</fullName>
            <ecNumber evidence="6">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_61748</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the hexokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="6"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375952.1">
        <property type="nucleotide sequence ID" value="XM_007375890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APA5"/>
    <dbReference type="STRING" id="619300.G3APA5"/>
    <dbReference type="GeneID" id="18874031"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61748"/>
    <dbReference type="eggNOG" id="KOG1369">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014393_4_1_1"/>
    <dbReference type="InParanoid" id="G3APA5"/>
    <dbReference type="OMA" id="PDFQPFE"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00180"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019158">
        <property type="term" value="F:mannokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24000">
        <property type="entry name" value="ASKHA_NBD_HK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF24">
        <property type="entry name" value="PHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="4 6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="8">
        <location>
            <begin position="75"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="9">
        <location>
            <begin position="276"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="542" mass="60403" checksum="CFBCA2ACD5A2955D" modified="2011-11-16" version="1">MTTNTTPAAISITPSMKEVEGLGLNIGEFTLEESPSNSSVSACSEESESPLTSQAGSDSKMESLLSSIVKSFTQTLTNENLSSQSDLLHADFDSALSDNSNITMLPNYNIQPTGQEHGQFLVIDLGGSTLRIAVVNIDQPVDAEETDRSSRIQTVIEKSWIIPNDFKVIDWEFFKFIGAKIKSVLELQEVLDINSIVKTGITWSFPLETTDYNHGRIVHVSKGYTIHPDIFGKDLKVILESVLKDEYAIDIDIRIIFNDSLAVYAAGAFIDKNTKLALVLGTGLNVCCSLDSSSKVHPDKVLNGHDRILYNTELSLFGQNLVTPNFVTKYDSHIDSRFGIFNQHFQSFMTVDPNTGTIFQPHELMASGRYLPELTRLVLVDLIEAQEIFVDRSQYELTEILTNEYDGFDGELMCFINESDDFEAINKKVSERYNWKTGISQEEITILKNVTNAIIHRAAFIVAYTIVSFVKLLHEHNANEDLSEVTIGYVGSVLYYFNSYRKLVKQLINDNEDAKKLGLKVDLKLIDNSSIIGAAIGAAYYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3APB0</accession>
    <name>G3APB0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Chromosome transmission fidelity protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_50681</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CTF8 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375363.1">
        <property type="nucleotide sequence ID" value="XM_007375301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APB0"/>
    <dbReference type="FunCoup" id="G3APB0">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APB0"/>
    <dbReference type="GeneID" id="18871473"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50681"/>
    <dbReference type="eggNOG" id="KOG4487">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090690_1_0_1"/>
    <dbReference type="InParanoid" id="G3APB0"/>
    <dbReference type="OMA" id="QRPLPIM"/>
    <dbReference type="OrthoDB" id="121932at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018607">
        <property type="entry name" value="Ctf8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28605:SF1">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28605">
        <property type="entry name" value="CTF8, CHROMOSOME TRANSMISSION FIDELITY FACTOR 8 HOMOLOG (S. CEREVISIAE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09696">
        <property type="entry name" value="Ctf8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="16917" checksum="F37013222F41FBCD" modified="2011-11-16" version="1">MPTASIDCSQAKDILNSDGQLLPHQDNLISTPLGLTILEIQGELNLPTHVPNVAITESNREYIENFIKVDEIYDAVKFGRLEIDADETKVTLFIGKSQRLIGNIVKLDHPLAVLRVPVKTDDMTDDDEKIRLVDIVKKKMIFKQRPLPIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3APB8</accession>
    <name>G3APB8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histone-lysine N-methyltransferase SET5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">SET domain-containing protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_61174</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-lysyl-[histone] + S-adenosyl-L-methionine = N(6)-methyl-L-lysyl-[histone] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10024"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:9846"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:10025"/>
        </physiologicalReaction>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375371.1">
        <property type="nucleotide sequence ID" value="XM_007375309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APB8"/>
    <dbReference type="FunCoup" id="G3APB8">
        <property type="interactions" value="679"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APB8"/>
    <dbReference type="GeneID" id="18873759"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61174"/>
    <dbReference type="eggNOG" id="KOG2084">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031650_0_0_1"/>
    <dbReference type="InParanoid" id="G3APB8"/>
    <dbReference type="OMA" id="LMAMYQQ"/>
    <dbReference type="OrthoDB" id="438641at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042799">
        <property type="term" value="F:histone H4K20 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045814">
        <property type="term" value="P:negative regulation of gene expression, epigenetic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46402:SF2">
        <property type="entry name" value="HISTONE-LYSINE N-TRIMETHYLTRANSFERASE SMYD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46402">
        <property type="entry name" value="SET AND MYND DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="7">
        <location>
            <begin position="111"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00042380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="415" mass="46868" checksum="DE1AF23369F2D7CE" modified="2011-11-16" version="1" fragment="single">MTLDQESLAKNVEVISINDSKPEETGPVVPHERQIVDAVIAIWKEDPATESLGITKLHGLVKAKHPNWSVSEKRVKTLLKKFGLLSSSSNEQFTYASEIKSENTPDIELPSKVQIVMTSKRGKGLYAKHNIAKGELIWEEEPLFFIPPLANINLIKSGTACSHCGRLLQQSEGNTVLRGLDCQVCAEVWCGTKCKKLDGVLHGLLKHNVYNPNAKRSKLFDPEAFLHMQDYCLEDQWNALYAITLIYADILNDKSGTKAKQFKAMARVSQDIRYKALNSSAGAFDTLSGGALFVQEQQENLWKLGYEKFLRIFPSNPIDYQEFMYMMGTYNINNLDSNVFLIQSHLNHNCNPNTSVETELLRTDGLKVYAARDIRAGEELTTTYVNPKNTVQQRQRELRVNWGFLCGCEKCKEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3APQ4</accession>
    <name>G3APQ4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Respiratory supercomplex factor 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_61305</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375500.1">
        <property type="nucleotide sequence ID" value="XM_007375438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APQ4"/>
    <dbReference type="FunCoup" id="G3APQ4">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APQ4"/>
    <dbReference type="GeneID" id="18873833"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61305"/>
    <dbReference type="eggNOG" id="KOG4431">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087356_1_0_1"/>
    <dbReference type="InParanoid" id="G3APQ4"/>
    <dbReference type="OMA" id="QRWIREL"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098803">
        <property type="term" value="C:respiratory chain complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097250">
        <property type="term" value="P:mitochondrial respirasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010155">
        <property type="term" value="P:regulation of proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007667">
        <property type="entry name" value="Hypoxia_induced_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050355">
        <property type="entry name" value="RCF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12297:SF3">
        <property type="entry name" value="HIG1 DOMAIN FAMILY MEMBER 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12297">
        <property type="entry name" value="HYPOXIA-INDUCBILE GENE 1 HIG1 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04588">
        <property type="entry name" value="HIG_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51503">
        <property type="entry name" value="HIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="HIG1" evidence="10">
        <location>
            <begin position="4"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="121"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="156" mass="18330" checksum="0A4EC8F64A9F114E" modified="2011-11-16" version="1">MSTRLPSSVSFDEEEEEDFFKKIWARCKENPFVPAGTLATTGAITLAAFSMHKGNRKKTNIYYRYRVLFQGLTLVALVVGGLFFQKEAQGSQKSKEDLLREKAKKREQLWIEELERRDAIIQARKLRLQQSKDELRELAREEFEKERLLEDTEKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3APR8</accession>
    <name>G3APR8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein HIR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_139052</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Required for replication-independent chromatin assembly and for the periodic repression of histone gene transcription during the cell cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the WD repeat HIR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375515.1">
        <property type="nucleotide sequence ID" value="XM_007375453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APR8"/>
    <dbReference type="FunCoup" id="G3APR8">
        <property type="interactions" value="438"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APR8"/>
    <dbReference type="GeneID" id="18870175"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139052"/>
    <dbReference type="eggNOG" id="KOG0973">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004372_3_0_1"/>
    <dbReference type="InParanoid" id="G3APR8"/>
    <dbReference type="OMA" id="RGSWDGD"/>
    <dbReference type="OrthoDB" id="1741719at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000417">
        <property type="term" value="C:HIR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031120">
        <property type="entry name" value="HIR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011494">
        <property type="entry name" value="HIRA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019015">
        <property type="entry name" value="HIRA_B_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13831">
        <property type="entry name" value="MEMBER OF THE HIR1 FAMILY OF WD-REPEAT PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13831:SF1">
        <property type="entry name" value="PROTEIN HIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07569">
        <property type="entry name" value="Hira"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09453">
        <property type="entry name" value="HIRA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0678">Repressor</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 10" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Protein HIRA-like C-terminal" evidence="12">
        <location>
            <begin position="629"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="392"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="472"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="397"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="416"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="485"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW32239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="913" mass="101579" checksum="F7B71D429CF5DC5E" modified="2011-11-16" version="1">MKLLQFPLGHFNTVSVSPDNTKLIAGGDSVLQVYDMNGLIKYAQLDPAPKLDDLKTIKPVRTITCHKDAVNCVQFFHNSEEFISADTAGYIYHHTSSSAVQIYPFGSKTAVAIVDLSISADDKILAWSTTSGEVNLYSFPSHKYQRLTSSSSIQRSVSFDPTNNYLVTIGDDTLIHVYQYTQSPYKFRHISKISRLLNKNRRNVKYKRISWSPEGELVAVPTACKNQTMVISLISHTNNWNTSISLVGHDSTCQVVKFNPKLYGEDDSVYSVVASGDGDGTVCVWNTTKDSPIIILKNVGDDIKDLCWVGSNAVVWCGGNGIYVGKFEQSELGDEVDVKAFERLMSLQKSLVKDITHKYDENYKRGAKEIDYIEEVNGIDIRHEEKEVKVDVEPTSVVPTDSESSNVKGKITPSVLLPPDPTATPPPTDILDSAMSTRKSPAKTKSTKPAPSAQPIITMKNGKRRIQPMLISSNNSTPVLDHKTTTNTSAQKPVTSMEFDKPSTVISTDHVKKRPNQDQQQPSKKRQQLEPIKFIGTPIVNPSTAISQIRLATPKIRLHFQSKSGELTLDIKNGSGNETQPSRVTCIKKEKPVWTDFLAKHVGLVTSGNDFFAIALADGNIIIYSQISGIRVFPTLVLGSPISFLESCENYLMAVTNIGELFVWDISNKKSVLTSTITPLLDLGKYDDDGLSKSDHITLCAITTTGIPLITLSNGNGYMYNTSLAIWQTVSESWWCFGSHYWNSATTTTPTDSIIDMLEHHTNEEILRKTRTGRGKLFNKISKNMIMKEGFESLENTISINHLENRIKCAEILGEKQEFKKWFMIYVERLCELGLKVRLFEVCSELLGPNESQGNGSKEIAIMNNGNTTSSEWNPNICGWDKHTLLKDIITTCSIHRDAQRVLIHFGELLALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3APY3</accession>
    <name>G3APY3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_139054</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375580.1">
        <property type="nucleotide sequence ID" value="XM_007375518.1"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APY3"/>
    <dbReference type="GeneID" id="18870176"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139054"/>
    <dbReference type="eggNOG" id="KOG1293">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_254915_0_0_1"/>
    <dbReference type="InParanoid" id="G3APY3"/>
    <dbReference type="OMA" id="ITCTAQI"/>
    <dbReference type="OrthoDB" id="5559898at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="661"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1063"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1122"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1205"/>
            <end position="1286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="667"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1073"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1207"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1231"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1274"/>
            <end position="1286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1286" mass="146039" checksum="21182EA4C7667A84" modified="2011-11-16" version="1">MPVEFINSSSQNVGDVDFNELRVSIIGNKDLKYKLVGDVEFISRLVDEFNQSILEIVEKKKIAIGSSGGVVQNFQFLHELEKKAVILNILCTFIIEVNTNKNDNITKCLRLMEQVINPLIHLLNYFVEKFVPQLYMHPQIYADGNDEDDVIRKVENLIGYSLDILLGLSNIRNHNINSSMLWRFITSLLIVTDDSSISSTATTTTTTQNIFQINSCLLIKSLKLVPLLLSQASLSNNSITTLLTALLNRLSKECDIIIATHFLDISKSSTLLHQLAFENSCLPNIELNKPLLRRVVDVNLLLELIVCTAQIFSFSKFNDYDILMAAPDTQSKSSSEVVLSINVYLSLLLLVKYEDKQLNLAALNLICFYLNNLRPAKDIDDEMIFKSYKKLLPRIIAMLELEEDFTGAVSSSTALNTSSSSNILSSAFNKKQQSRSSGLSSELPIFLSSPGKILADLCAQYPLLNDEINDANIDYKLVKKLETNFKSSQLLKALKILKRDSKNGKVLVDFTLLLTVERDDNCLADLLLLLSVYTSGTEEYRARIINRPQENVKNNNNINLTNIIFQIIDNYHFLLTQVQLIYKLLYPKKGKQKPIIPKNDLPWFGKNLGIIRSLMDSAIYTNCFYFIRSLSRSVSSLRTFFVECNALTSFISDETNANTGTKIIPPSTTGSHSMASSGSVSIGGSASSGISSSASSSTSPGGLIINLLEIIRVYENLDQMLDFFNNLNKRSGESNSSSMNKRAQMTNKSICIGLLANFILDFSSFRYAIIGYENFLSSLSIIYQNSSTDFDKSLKFDQLTEEEIFQRNEIQLKILQVIKNFMFNETTENKQELMDYFALIFILKKASYGIGDYNFDANESVDITKLKLEQKLVAFDILRNFTAGSPKFNTVLINTYEQEFVKSEGNGKLPGKWFKFLSSNIRNVKLFIPNASPKESFDNDETLVKLIENDDYVSLVRSINYIEDHKYTIIDKIKRELFPNDELLKIWLRLLSFFIPESLEKKFDLNTKIVLNSNLNLIKSSITWIILNLTWKYSTYTFNVHDYTDYEVYQTVESSRHVTTNQRIYIDESDDNDEKREKEKPEPEESDSNDDELSVRQRAKYLDKLGFTAVIVKLMNYYSKQASSSDEAKSGKPGTSSSSYSALTPKRFDIQNSHDISEKLKTALRQITSLLRTKNSGVGGLIGKKSMGISKNEIRIRLNEDRNGVEVVEEHDEEDPAVYRRHHNRRSHVESEEEDDEAEEEAEEEEEEDDDDDDDDDEDDTEAGVGLYTPSDDFYSDDDDPVENWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ25</accession>
    <name>G3AQ25_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_50910</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375622.1">
        <property type="nucleotide sequence ID" value="XM_007375560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ25"/>
    <dbReference type="STRING" id="619300.G3AQ25"/>
    <dbReference type="GeneID" id="18871498"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50910"/>
    <dbReference type="eggNOG" id="ENOG502T6G2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028609_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQ25"/>
    <dbReference type="OMA" id="RPINHET"/>
    <dbReference type="OrthoDB" id="6375767at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="421"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="490"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="603"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="285"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="326"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="374"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="636"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="678"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="695"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="718"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="750"/>
            <end position="766"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="766" mass="83687" checksum="9BD4C2A8D506B17C" modified="2011-11-16" version="1">MSTSPSQKKVDDFLSSLSQLSQDRIKQDQQRQRDLQRNIDDLRHSRTPSPVKGVASTSKSSYPIDIPELKFNRGVQELNLNLLTPVARKHEKTPPVVKPKPNITPAVLPRAEKGSGMRSFGQMEQLIKQGTTPEGVYASDPAERTEPAVGGTKVSSPPPLQATPRGQWRNTSIPTQPPEKEEERRKEKEEEDDDDEKPPPPPIRTVKPTYLSSATQPPKVPSKSPSLAPKPGKKDWLTSTLDNSKTKATSSASDIPRPPAKSPTISQTPKPPAKKDWLSSTLDNSKTTVTPTDTPKVPTKSPTLSQISKSPTKKNWLTSTLDNSKTTITLSTSESPPSPPKHKSDWLSSLAHSKTTTSTPQSSESKASPPPVAKKPTSWISSAVKSESSKSLAQIANPSPERKISTKKGNWIDSAVRRTNGVQRELPEKPAYLSNIPKSKSTKVLEEPEQEKEPEFIARRETVLRKTPVPPPVPKKIASSAEKVVEFKTAKLRSASPEKSSTPESAELSEFHSKLNKLRSQSPERKPVEKSSEEKEFLVKFKTVKSTAPPPPLKPKPAAVYNAPPAEFQKRFDQVVANGPKVSLKPTAKNEYARKDAEALQAQLKSLSKPAKPAKGQNSYANKDAEVIQNQLSRLAKPEPAEPAKAETFESKLNGLLSRVNSAPATPPAPSPAGIRRVKTEPANDSPKTEAKLSHPGKGRSKGPKRRLPKSLATSGAPPQKQQEQQEQQPIDISIKKPAPPINKHSKPKPVGELKPSRDFSGELFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQG4</accession>
    <name>G3AQG4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MTHFR SAM-binding regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_51518</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methylenetetrahydrofolate reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376289.1">
        <property type="nucleotide sequence ID" value="XM_007376227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQG4"/>
    <dbReference type="FunCoup" id="G3AQG4">
        <property type="interactions" value="440"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQG4"/>
    <dbReference type="GeneID" id="18871562"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51518"/>
    <dbReference type="eggNOG" id="KOG0564">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025841_2_2_1"/>
    <dbReference type="InParanoid" id="G3AQG4"/>
    <dbReference type="OMA" id="AWKEEFY"/>
    <dbReference type="OrthoDB" id="16284at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004489">
        <property type="term" value="F:methylenetetrahydrofolate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00537">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.220:FF:000002">
        <property type="entry name" value="Methylenetetrahydrofolate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004621">
        <property type="entry name" value="Fadh2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003171">
        <property type="entry name" value="Mehydrof_redctse-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053806">
        <property type="entry name" value="MTHFR_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00677">
        <property type="entry name" value="fadh2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754:SF3">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE (NADPH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02219">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21895">
        <property type="entry name" value="MTHFR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MTHFR SAM-binding regulatory" evidence="9">
        <location>
            <begin position="308"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="614" mass="69728" checksum="2941DF4D413D81C2" modified="2011-11-16" version="1">MKISQKLQQAHAGPNPPPATFSFEFFVPKTSQGVQNLYDRMDRMYDLNPIFIDITWNAGGRSSTLTNEMVYTAQSTLGLETCMHLTCTNMKVELIDEALDKAFKSGCQNILALRGDPPLDGSESTGDFKYAKDLIKYIRKKYGDHFNIGVAGYPEGHPEEESEIKTLHYLKEKCDVGADFIVTQMFYDVDNFIDWCKKIRKIGVEIPIIPGIMPISTYAAFVRRAKWSEIAIPQHFLDVLDPIKDDDYLVRERGTELVSDMCIKLLHSGYVNHLHFYTMNLEKATVMILEKLNLIEAVKSHDVVGSTELPWRKSLHPGRSKESIRPIFWQNRKYSYVTRTATWDEFPNGRWGDSRSPAFGDIDLCATELLRQSPKRALELWGTPQSLKDMSDIVVSYLQGDLKALPWSDGPVGDDTKIIVDDLIHLNKKGFFTMNSQPSLNAIKSSEKVFGWGPKNGYVYQKQYLEFFCHKDVIAKLLYKIDSFNTKEGDTSSSVISYYAVDKDGHLTTNCKDDDINAVTWGVFPGEEILQPTIVEKTSFLAWKDEVYRLFSEWGNVLGSNLAGADPEKIKGFLDLMNTLSNDFVLCNLVNNDFIDDNSTIFKLIEELDVKDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQH8</accession>
    <name>G3AQH8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">D-aminoacyl-tRNA deacylase</fullName>
            <ecNumber evidence="2 6">3.1.1.96</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_62108</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a D-aminoacyl-tRNA + H2O = a tRNA + a D-alpha-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>glycyl-tRNA(Ala) + H2O = tRNA(Ala) + glycine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the DTD family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="2 6"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376302.1">
        <property type="nucleotide sequence ID" value="XM_007376240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQH8"/>
    <dbReference type="FunCoup" id="G3AQH8">
        <property type="interactions" value="1604"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQH8"/>
    <dbReference type="GeneID" id="18874187"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62108"/>
    <dbReference type="eggNOG" id="KOG3323">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076901_0_4_1"/>
    <dbReference type="InParanoid" id="G3AQH8"/>
    <dbReference type="OMA" id="VFGADMK"/>
    <dbReference type="OrthoDB" id="275783at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097358">
        <property type="term" value="F:D-leucyl-tRNA(Leu) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051500">
        <property type="term" value="F:D-tyrosyl-tRNA(Tyr) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106026">
        <property type="term" value="F:Gly-tRNA(Ala) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900832">
        <property type="term" value="P:D-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900829">
        <property type="term" value="P:D-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00563">
        <property type="entry name" value="Dtyr_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.80.10:FF:000001">
        <property type="entry name" value="D-aminoacyl-tRNA deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.80.10">
        <property type="entry name" value="D-tyrosyl-tRNA(Tyr) deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00518">
        <property type="entry name" value="Deacylase_Dtd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003732">
        <property type="entry name" value="Daa-tRNA_deacyls_DTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023509">
        <property type="entry name" value="DTD-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00256">
        <property type="entry name" value="D-aminoacyl-tRNA deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10472:SF5">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10472">
        <property type="entry name" value="D-TYROSYL-TRNA TYR DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02580">
        <property type="entry name" value="Tyr_Deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69500">
        <property type="entry name" value="DTD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="163" mass="18006" checksum="D8274D656A782090" modified="2011-11-16" version="1">MRVVIQKVKSASVTVEEKVISSIGKGLMLLVGVSTHDTMDDVAKLSKKLLSLRVFEDLSQPPETATKWYGKPWAKNVVDIEGEILSVSQFTLYGTIKKGTKPDFHKAAKGHHAIELYESFLDQLRQGLGQDKVKDGEFGAMMDVALVNDGPVTIIWDTKDMSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQJ7</accession>
    <name>G3AQJ7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Protein transport protein SEC13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_141001</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPII-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat SEC13 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376323.1">
        <property type="nucleotide sequence ID" value="XM_007376261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQJ7"/>
    <dbReference type="FunCoup" id="G3AQJ7">
        <property type="interactions" value="1112"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQJ7"/>
    <dbReference type="GeneID" id="18870336"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141001"/>
    <dbReference type="eggNOG" id="KOG1332">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032441_0_1_1"/>
    <dbReference type="InParanoid" id="G3AQJ7"/>
    <dbReference type="OMA" id="IWKEEGD"/>
    <dbReference type="OrthoDB" id="10261782at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061700">
        <property type="term" value="C:GATOR2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090114">
        <property type="term" value="P:COPII-coated vesicle budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051664">
        <property type="term" value="P:nuclear pore localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902953">
        <property type="term" value="P:positive regulation of ER to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070863">
        <property type="term" value="P:positive regulation of protein exit from endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032527">
        <property type="term" value="P:protein exit from endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000017">
        <property type="entry name" value="SEC13 homolog (S. cerevisiae)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037363">
        <property type="entry name" value="Sec13/Seh1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN SEC13 / SEH1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024:SF2">
        <property type="entry name" value="PROTEIN SEC13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="13" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="6 16" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="50"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="203"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW31545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="299" mass="33520" checksum="05C63A71D22EA625" modified="2011-11-16" version="1">MVTVANAHDDLIHDAVLDYYGKRLATCSSDKKIKIFEIEGTENYKLSTTLTGHEGPVWQVSWAHPKFGSILASCSYDGKAFIWKETPETQTWSIIAEHTVHQASVNSVSWAPHELGAVLLCTSSDGKVSVVDFNEDGTTSHVIFNAHNIGVNSASWAPISNVSSKDPQTLKQHRRFVTCGSDNLAKIWKYDSVHNTYVEEARLEGHTDWVRDVDWSPSILVRSYIATASQDRTVLIWTQDRDGHWQKQLLTEEKFPDVCWRCSWSLSGNILAVSGGDNKVSLWKENLQGKWESAGEVDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQW7</accession>
    <name>G3AQW7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein GLO3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">GLO3</name>
        <name evidence="4" type="ORF">SPAPADRAFT_61776</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375974.1">
        <property type="nucleotide sequence ID" value="XM_007375912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQW7"/>
    <dbReference type="FunCoup" id="G3AQW7">
        <property type="interactions" value="725"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQW7"/>
    <dbReference type="GeneID" id="18874041"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61776"/>
    <dbReference type="eggNOG" id="KOG0706">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023062_7_0_1"/>
    <dbReference type="InParanoid" id="G3AQW7"/>
    <dbReference type="OMA" id="KAGGIWE"/>
    <dbReference type="OrthoDB" id="983479at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048205">
        <property type="term" value="P:COPI coating of Golgi vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686:SF4">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE ACTIVATING PROTEIN 3, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE ACTIVATING PROTEIN 3, ISOFORM H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="143"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="229"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="19"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="148"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="242"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="257"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="277"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="337" mass="35958" checksum="89092EEA9A8BB9F6" modified="2011-11-16" version="1" fragment="single">MADAIKHPDVVTLEDTTDNLEISDSASESTDDFFSNWTKPVNSTPSPITSKNVSPSVSTDDLTKKKPITTTPRTSTTTTTTTSSRLKTTSSAVGGAKKSILLSKGNGPRSSRLAAKRINKSEPADIDFEELEKQAIKEAEEAAKLGYNPNENLTPTPAPAATLPKKSLSLGKSNDDVVLTPAPIQETTQQFQKLGFGMVQGENNVSSGPSKQYKEVKYTGEVSNKYGTQKGISSDEFFGRGPRFDEQAKNEARSKLQAYSGAQSISSSSYFGEEEGANGGRPGGRGRSGTGGLADIEASAREFASRFSGNANQDLDVLKDALEEGANKLGSYLRDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR96</accession>
    <name>G3AR96_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61840</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376049.1">
        <property type="nucleotide sequence ID" value="XM_007375987.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR96"/>
    <dbReference type="FunCoup" id="G3AR96">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR96"/>
    <dbReference type="GeneID" id="18874077"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61840"/>
    <dbReference type="eggNOG" id="KOG4539">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043838_0_0_1"/>
    <dbReference type="InParanoid" id="G3AR96"/>
    <dbReference type="OMA" id="PGFYLTY"/>
    <dbReference type="OrthoDB" id="5562676at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018786">
        <property type="entry name" value="Mit_KHE1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28062">
        <property type="entry name" value="K+-H+ EXCHANGE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28062:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10173">
        <property type="entry name" value="Mit_KHE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="363" mass="41822" checksum="1130BED8F34AEDF2" modified="2011-11-16" version="1">MLIPTRSSRYANLGINYARRFQSNVAAGAAAAADKKILKKNSIFVISIPITTSKSYIYCNHRPGKMLAESQLKTIPPLIKLEHKLIKLAIKGWTKLSTSEKSINVKITNLVKQLMRTIAYEENCLRSFPNQKAMIREINEELIESKHPNLVQSEIEKLDISHDQLKPIPLYHPDFQNPTTILNQIYDFRKDNQIKHKKYAILCAIGIPLSLPFALVPIIPNVPGFYIAFRLYCHIKALMGIKHLDYLLETTPSQVRTIQREQEKHDDEEMDEVKLADESTVVETKHLRFQSVAEMDQIYAEHNEKRHLNELVQEDECILINESIINSLVDQLDLEHLREDLLKALEQETKRLNKDIRVGDAVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARF7</accession>
    <name>G3ARF7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_61845</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PGA52 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376056.1">
        <property type="nucleotide sequence ID" value="XM_007375994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARF7"/>
    <dbReference type="FunCoup" id="G3ARF7">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARF7"/>
    <dbReference type="GeneID" id="18874080"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61845"/>
    <dbReference type="eggNOG" id="ENOG502QSI7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030276_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARF7"/>
    <dbReference type="OMA" id="NQDMPAI"/>
    <dbReference type="OrthoDB" id="118256at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018805">
        <property type="entry name" value="YJL171C/Tos1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018807">
        <property type="entry name" value="YJL171C/Tos1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31737">
        <property type="entry name" value="PROTEIN TOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31737:SF2">
        <property type="entry name" value="PROTEIN TOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10287">
        <property type="entry name" value="YJL171C_Tos1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10290">
        <property type="entry name" value="YJL171C_Tos1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442528" description="glucan endo-1,3-beta-D-glucosidase" evidence="9">
        <location>
            <begin position="21"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="Cell wall protein YJL171C/Tos1 N-terminal" evidence="11">
        <location>
            <begin position="36"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Cell wall protein YJL171C/Tos1 C-terminal" evidence="10">
        <location>
            <begin position="200"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="144"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="155"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="167"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="440" mass="46128" checksum="27B1B386B4756A72" modified="2011-11-16" version="1">MKFSYTTLLTILSSIVAVSADCENVGGNYYCSATKKVVYKGVGFQGTYQDVTNMDDTTGTCSQQPYSFSGNLSPLDEELSVHCRGPLKLLQFGVYYPSGSNNQKREQEDCTTRHIHHKHKRGVEIVEVTETVLVDAQGNTITQSTTQATTVEGTLPTTYSNNNEGQTESQPPQPSTSAAPSPSSSSSPSAAPSEAPSDSAWVRSSYYTPGSADNCVFLNYHGGAGSGVWSSAFGNSLSYANSDNSGGSSSPVALGDVTIGSNSEYVIMAGDDCSQGSGCGYYRPGSVAHHGFGGASKIFVFEFEMPSDGSSGFNGDMPAIWLLNAKIPRTLQYGDASCSCWKTGCGELDLFEILSSGSNKLISHLHSGQGASQDTNNGGGGSQDYFVRPTSGSLKAAVVFTGSDIHIVQVDDSTDFSAGLSADVVNGWVQESGSVASIGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARJ6</accession>
    <name>G3ARJ6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_139914</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376095.1">
        <property type="nucleotide sequence ID" value="XM_007376033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARJ6"/>
    <dbReference type="FunCoup" id="G3ARJ6">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="GeneID" id="18870251"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139914"/>
    <dbReference type="eggNOG" id="ENOG502QPTQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007127_6_0_1"/>
    <dbReference type="InParanoid" id="G3ARJ6"/>
    <dbReference type="OMA" id="VCFPMVP"/>
    <dbReference type="OrthoDB" id="29879at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010961">
        <property type="term" value="P:intracellular magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12829">
        <property type="entry name" value="Alr1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.340:FF:000008">
        <property type="entry name" value="CorA family metal ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.20">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044089">
        <property type="entry name" value="Alr1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21535">
        <property type="entry name" value="MAGNESIUM AND COBALT TRANSPORT PROTEIN/MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21535:SF55">
        <property type="entry name" value="MAGNESIUM TRANSPORTER ALR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="682"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="713"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="561"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="592"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="127"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="561"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="607"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="783" mass="87790" checksum="A723CAA435442C3C" modified="2011-11-16" version="1">MSDTESLRSNTHHMPKTDEVLDDHRNHLPIIHPQQGSSDIYKGTSVAITDSEDEEPGVTIAPPPKRRPSKKMPRRSSQSHGNPPSSSSKSAYMNTLRKDFYLNEEDSSPPILKSTPRKKTRAYSNSQQQQQQQQQQQQQQLPHHYHVDEPSSDLVSPLTKYKTNDSDASYTRGGRRPSRSSIDSEADSHASRSSQETEEDVCFPMSREHIRINGIDFDEIEEFIKEERELIAHSVMERNESRNHSMATGNAASIAQQQSSAASMKYTPKNILKKTQTTLTKIKSHWDPSSSSSDPDRELYYNIDEKDHGFDDSSSSSEQVRFGGARITDGSTSSLPQRFSFFHSESDETVHAADIPSLVAPGQSVCDLFRNGEETWWLDCSCPTDAEMKMLAKAFGIHPLTAEDIRMQETREKVELFRSYYFVCFHTFEPDKESEDYLEPINVYLVVFREGILTFHFSPISHPASVRRRVRQLRDYVDVSADWLCYALIDDITDGFAPVITGIEYEADAIEDFVFVARDSDFSNILQRIGESRRKVMTLMRLLSGKADVIKMFAKRCQDEVQQAQASSQPQSQVSPNQSFSGYPVGFNQGGTGGVSFGPGPTPLTPSQPANTVTHTSSAGLGSGNTDIALYLGDIQDHVVTMFQNLLSYEKIFSRSHSNYLAQLQVKSFNANNKITEMFSKVTLIGTMLVPLNLITGLFGMNVLVPGQNADNLGWWFGILGVLIVVILSSFVFARWWIGKLNSKMDGGEGVVLSARKSIKSLGLRRNRGKDARSMVSFPNKYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARR7</accession>
    <name>G3ARR7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Presequence translocated-associated motor subunit PAM17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_61905</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the PAM complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the PAM17 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376112.1">
        <property type="nucleotide sequence ID" value="XM_007376050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARR7"/>
    <dbReference type="FunCoup" id="G3ARR7">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARR7"/>
    <dbReference type="GeneID" id="18874104"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61905"/>
    <dbReference type="eggNOG" id="ENOG502S1B1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068297_2_0_1"/>
    <dbReference type="InParanoid" id="G3ARR7"/>
    <dbReference type="OMA" id="MIFGFDP"/>
    <dbReference type="OrthoDB" id="5970083at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013875">
        <property type="entry name" value="Pam17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28021">
        <property type="entry name" value="PRESEQUENCE TRANSLOCATED-ASSOCIATED MOTOR SUBUNIT PAM17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28021:SF1">
        <property type="entry name" value="PRESEQUENCE TRANSLOCATED-ASSOCIATED MOTOR SUBUNIT PAM17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08566">
        <property type="entry name" value="Pam17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="178" mass="20473" checksum="2E6DB6528392A5F5" modified="2011-11-16" version="1">MFRIGVHNRAFTRRAFGQLRFNSTKAPGEFTWVDYFQLKKQNSRLNMFSGAVTGVLGSALTLGYLGNIEIDVEKPIFGFDPLMVMGGTVILGGLVGYLVGPFMGSAVFKMMHRSKMAQFEVRNKEFLQRLRRNRPDPSSQSFSNPIPDYYGEKIYSLKDYQQWLRDCNAFRRKAKEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARS0</accession>
    <name>G3ARS0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">coproporphyrinogen oxidase</fullName>
            <ecNumber evidence="4">1.3.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_61910</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aerobic coproporphyrinogen-III oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.3.3" evidence="4"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376115.1">
        <property type="nucleotide sequence ID" value="XM_007376053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARS0"/>
    <dbReference type="FunCoup" id="G3ARS0">
        <property type="interactions" value="585"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARS0"/>
    <dbReference type="GeneID" id="18874105"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61910"/>
    <dbReference type="eggNOG" id="KOG1518">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026169_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARS0"/>
    <dbReference type="OMA" id="VHANWRY"/>
    <dbReference type="OrthoDB" id="15318at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00322"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004109">
        <property type="term" value="F:coproporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1500.10:FF:000006">
        <property type="entry name" value="Coproporphyrinogen III oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1500.10">
        <property type="entry name" value="Coproporphyrinogen III oxidase, aerobic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001260">
        <property type="entry name" value="Coprogen_oxidase_aer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036406">
        <property type="entry name" value="Coprogen_oxidase_aer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018375">
        <property type="entry name" value="Coprogen_oxidase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003727">
        <property type="entry name" value="PRK05330.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10755">
        <property type="entry name" value="COPROPORPHYRINOGEN III OXIDASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10755:SF0">
        <property type="entry name" value="OXYGEN-DEPENDENT COPROPORPHYRINOGEN-III OXIDASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01218">
        <property type="entry name" value="Coprogen_oxidas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000166">
        <property type="entry name" value="Coproporphyri_ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00073">
        <property type="entry name" value="COPRGNOXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102886">
        <property type="entry name" value="Coproporphyrinogen III oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01021">
        <property type="entry name" value="COPROGEN_OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="323" mass="36936" checksum="75A571EAE352C307" modified="2011-11-16" version="1">MVTKEQIHDSSLPVRERMEALIRWKQQEITSALAALDTVTFHTDKWTRGNTGGGGESMVLQNGTTFEKGGVNISIVHGKLPHEAVTRMKADHSNLKESAKDGSVDFFACGLSMVIHPINPHAPTTHANYRYFETTDPETKEVQAWWFGGGADLTPSYLYEEDAKHFHQQHKDALDKFDKDLYPKWKKWCDEYFFIKHRGETRGVGGIFFDDYDEKSPDEILKIVESAFDAFIPAYCPIIAKRKDTPYTPEQKQWQQIRRGRYVEFNLVLDRGTQFGLQTPGSRVESILMSLPATAAWAYDHHPEKGSDEDKLLEVLKNPVEWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS37</accession>
    <name>G3AS37_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pseudouridine-5'-phosphate glycosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_56625</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376664.1">
        <property type="nucleotide sequence ID" value="XM_007376602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS37"/>
    <dbReference type="STRING" id="619300.G3AS37"/>
    <dbReference type="GeneID" id="18872056"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56625"/>
    <dbReference type="eggNOG" id="KOG3009">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012201_0_1_1"/>
    <dbReference type="InParanoid" id="G3AS37"/>
    <dbReference type="OMA" id="GVHREWT"/>
    <dbReference type="OrthoDB" id="198885at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004730">
        <property type="term" value="F:pseudouridylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1790.10">
        <property type="entry name" value="Indigoidine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01876">
        <property type="entry name" value="PsiMP_glycosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022830">
        <property type="entry name" value="Indigdn_synthA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007342">
        <property type="entry name" value="PsuG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42909:SF1">
        <property type="entry name" value="CARBOHYDRATE KINASE PFKB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42909">
        <property type="entry name" value="ZGC:136858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04227">
        <property type="entry name" value="Indigoidine_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110581">
        <property type="entry name" value="Indigoidine synthase A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW31886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="316" mass="33902" checksum="965B88C38E380D37" modified="2011-11-16" version="1">MPLRRLPIQISHEIKQALTDKLVPVVALESTIITHGLPFPKNVEMALSVEQTLRQNGAIPATCAFINGIPHIGLNHGQLQQLAESKATKVSRRDIGHVMANKLNGGTTIASTMILSHLAGIKVFSTGGLGGVHREGQLTMDVSADLTELGRTPVSVVCAGPKSILDIGLTMEYLETQGVYVGTYNEENEPNIEIPGFYCRKSGVLSPYSFKSFTEAASIIHNQNRLGLTSGNVFCIPPPKESALDSEYIAGIIDQANELAQVQGISGKQLTPFLLSQIAIQTQGKSVDCNLNLVYNNAKAGAEIGKELLALEKVCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASD1</accession>
    <name>G3ASD1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Histone demethylase JHD2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_51884</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376704.1">
        <property type="nucleotide sequence ID" value="XM_007376642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASD1"/>
    <dbReference type="FunCoup" id="G3ASD1">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASD1"/>
    <dbReference type="GeneID" id="18871603"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51884"/>
    <dbReference type="eggNOG" id="KOG1246">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000991_4_0_1"/>
    <dbReference type="InParanoid" id="G3ASD1"/>
    <dbReference type="OMA" id="YSIPEFY"/>
    <dbReference type="OrthoDB" id="1678912at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034647">
        <property type="term" value="F:histone H3K4me/H3K4me2/H3K4me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16100">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15543">
        <property type="entry name" value="PHD_RSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF33">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="11">
        <location>
            <begin position="15"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="ARID" evidence="10">
        <location>
            <begin position="81"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="9">
        <location>
            <begin position="185"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="12">
        <location>
            <begin position="331"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="744"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="745"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW30671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="828" mass="95833" checksum="EEAC7B8D7293AEB3" modified="2011-11-16" version="1">MPATTSFKKELLKPCPVLNPTEHEFNDPIGYLSSEPIAKLGSLYGIVKIVPPPNWKPPFNISPNFKFHVRKQKISDLGLTTRSRMFFKESINRFLKMRRKKQLRSSFKVNGVNIYYYDLFLEVEKLGGPENMNKAKWIEINKIMNVDPVSSAIEQEYIANVRYYAMYLHNKVDFEFPESDDEDEFDNCLICGKHDKPEETLLCDNCDNPYHMKCLPTPLTSIPAANWYCDKCLIGTGEYGFEEDQDIKYSIPEFYEMCHEFDAKFAAKYNNNEALTVDVIEDKFWSFVENEKVDIEVKYGADIHNLKPGEISGFPMKDTPGLDHNDPLTNHYIKHPFNLTKLPFAKGSLLNYVNSSISGMTVPWIYIGSLLSTFCWHVEDHYTLSANYCHFGATKKWYGIPAVLADKFEKVMRNSAPDLFQKQPDLLHQLVTLMSPTKLVEHGIPVTYADQNPGEFIITYPRVYHAGFNCGFNFNEAVNFTMSDWLEFGEKSIGDYKVIKKENVFNHYELLESILKSFNKQRGKISANQIDLVNRCLLSFQRFVVDQEFLLQSIDKSKFDCRFEPKYLKWKKEPNSNRAYDDDDDDDENHDDYACDSCKTHLGYQFCVLKCNLPEVKNIKSLAEGEQKQTSKELSPDVEIIKTNQLLTPNTSPLEEKLSSPKQEMIEVVANSEASHNLSSIGQKREAEMDAYDRLIQEAKRQARGTEEKVSAGRKSKRLETIPRKSLNEDELEKEYTARVAQLQTHKRKRKHHIQHPTHSMRMSMKSSKRTQRENKEIRLCLGCTKKFRGLTSNDQSVHGILIYRSYPSQLKDLIRTTKRNITEIQGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASE6</accession>
    <name>G3ASE6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SCF1</name>
        <name evidence="14" type="ORF">SPAPADRAFT_62966</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377097.1">
        <property type="nucleotide sequence ID" value="XM_007377035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASE6"/>
    <dbReference type="FunCoup" id="G3ASE6">
        <property type="interactions" value="404"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASE6"/>
    <dbReference type="GeneID" id="18874541"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62966"/>
    <dbReference type="eggNOG" id="KOG0756">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_5_0_1"/>
    <dbReference type="InParanoid" id="G3ASE6"/>
    <dbReference type="OMA" id="KDWYKGG"/>
    <dbReference type="OrthoDB" id="204711at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005469">
        <property type="term" value="F:succinate:fumarate antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000021">
        <property type="entry name" value="SFC1p Mitochondrial succinate-fumarate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049563">
        <property type="entry name" value="TXTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788">
        <property type="entry name" value="SUCCINATE/FUMARATE MITOCHONDRIAL TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788:SF2">
        <property type="entry name" value="SUCCINATE_FUMARATE MITOCHONDRIAL TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 13" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="17"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="115"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="217"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW31064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="321" mass="35353" checksum="344D6A12426603E1" modified="2011-11-16" version="1">MSDTAAKVPFKRQPPKKRGAVDFIAGGVAGLFEALVCHPLDTIKVRMQLYRKSGQKPPGFVKTGVNIAQKEGFLSLYKGLGAVIIGIVPKMAIRFSSYEFYRSLFYDENFNITTGQTFIAGVGAGITEAVMVVNPMEVVKIRLQAQHHSMKDPLDIPKYRNAPHAAYLICKEEGFSTLYRGVSLTAARQASNQGVNFTVYSKLKEYLQERSGTEVLPHWQTSGIGLISGALGPLSNAPLDTIKTRLQKTTFASNESGMVRIMKITKQLIREEGIHALYKGITPRIMRVAPGQAVTFTVYEFIKDMLNSDTSILASTNKKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASF8</accession>
    <name>G3ASF8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_156732</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377109.1">
        <property type="nucleotide sequence ID" value="XM_007377047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASF8"/>
    <dbReference type="STRING" id="619300.G3ASF8"/>
    <dbReference type="GeneID" id="18871056"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_156732"/>
    <dbReference type="eggNOG" id="KOG1601">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_470999_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASF8"/>
    <dbReference type="OMA" id="EMMIMDA"/>
    <dbReference type="OrthoDB" id="515401at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000007">
        <property type="entry name" value="Nitrogen regulatory AreA, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF281">
        <property type="entry name" value="BOX A-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="8">
        <location>
            <begin position="228"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="133"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="173"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="404"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="533"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="182"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="285"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="414"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="441"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="667" mass="73023" checksum="9214931272E3F352" modified="2011-11-16" version="1">MTSFTPQNNGVPHQNPDVNNNNTISDFEAGPTISDLFHSAKKLLNLQPRFENRQVRKVNSQAQMNHNLYATNSDNTSTSPNMIMPGHDNILDLLSPLSIEDLKNNSHHHHIHNNASRPIATTSTTMSTANFTSQRNEYKTPNSDYSSPGAPTRQQQQPQITHDAFIKPKSNLTSSLNKLNNRPSVQPTPIKSNTSPTSSTASTPISSSAPIAGQPPAAATATAAAAANQKPTECYNCKTLKTPLWRKDAEGNTLCNACGLFYKLHGTTRPLSLKTDVIKKRNSRKSTSQASSQKIAVAASPSTSNISASFKNEITNIRPKPVSTNSSSSLPNYGTTGMAISANQRYKNVLILPKPPSGNNLKSIPIPQASQAFSPSSPFTINSNQPFKRKKSEVNISSQMPMMTPADEATTPISSSFSSRNRAASSSSSLSNSLKRNSSFQNSTLNRRTSLTNMTRKLTNAVITPTNSLTSSNINILNQRFPQSSYFENPANQQVPNSLSRTSSHATLMNQANTIMENNQLGIEGVETPGSLNSSPSYQYQATDTPNSVPATPNVNDLLPSSKNQYPSDQQLEQVLEEYRASKPPISLHEGIDDEMIMLDTIVDEEEVRMLDNDDDEFFKNYTSLTNEFLIPTPKQQHSLNMVPQVNGNSNGSTNDYKDLDWLKFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASI0</accession>
    <name>G3ASI0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial</fullName>
            <shortName evidence="7">Glu-AdT subunit A</shortName>
            <ecNumber evidence="7">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">HER2</name>
        <name evidence="9" type="ORF">SPAPADRAFT_63024</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Subunit of the heterotrimeric GatFAB amidotransferase (AdT) complex, composed of A, B and F subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="7"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377131.1">
        <property type="nucleotide sequence ID" value="XM_007377069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASI0"/>
    <dbReference type="FunCoup" id="G3ASI0">
        <property type="interactions" value="358"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASI0"/>
    <dbReference type="GeneID" id="18874557"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63024"/>
    <dbReference type="eggNOG" id="KOG1211">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_7_6_1"/>
    <dbReference type="InParanoid" id="G3ASI0"/>
    <dbReference type="OMA" id="QPASYCG"/>
    <dbReference type="OrthoDB" id="421993at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF7">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="8">
        <location>
            <begin position="41"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="7">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="459" mass="49424" checksum="0989D4451B47900A" modified="2011-11-16" version="1">MRQQHRLCQRINPIRLVSTVRSTVPNNTHNVLISSTRVEPTTGQGPLAGSTYICKDNIVTKVGFTTSASKSLENYKSPFNATVVSLLAQAGSKLIGKSNLDEFGMGSSNTNSHFGPTMNPVYPDQRVPGGSSGGSAAAVAAGLADFSLGTDTGGSVRLPASYCSVIGFKPSYGRISRWGVVPYAQTLDTVGIISKSLNVVRKVFSVLDVYDEKDPTSLPQNIRNEFKPSTCKQLTVGIPSEFVLDELSPETRAAWSQALTKLQEAGHNIKPISIKSITKSLPAYYTLATAEAASNLSRYDGVRYGYNNNNQHTKDAMNLIVSNRSESLGPEVQRRIILGNYTLSSDSGDHYLKATKVRQELVEEFSSLFKTKHALVETASGDCDLMISPTSIGEAPTVEEFLNADNANFLNSYVNDILTVPASLAGIPAISIPFGKLGIQLMGQFGDDELVLQVAETLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASJ3</accession>
    <name>G3ASJ3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glutathione-specific gamma-glutamylcyclotransferase</fullName>
            <ecNumber evidence="1">4.3.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_142179</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.3.2.7" evidence="1"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376712.1">
        <property type="nucleotide sequence ID" value="XM_007376650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASJ3"/>
    <dbReference type="FunCoup" id="G3ASJ3">
        <property type="interactions" value="332"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASJ3"/>
    <dbReference type="GeneID" id="18870427"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142179"/>
    <dbReference type="eggNOG" id="KOG3182">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070703_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASJ3"/>
    <dbReference type="OMA" id="DHREKDG"/>
    <dbReference type="OrthoDB" id="1933483at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003839">
        <property type="term" value="F:gamma-glutamylcyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061928">
        <property type="term" value="F:glutathione specific gamma-glutamylcyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006840">
        <property type="entry name" value="ChaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12192">
        <property type="entry name" value="CATION TRANSPORT PROTEIN CHAC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12192:SF2">
        <property type="entry name" value="GLUTATHIONE-SPECIFIC GAMMA-GLUTAMYLCYCLOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04752">
        <property type="entry name" value="ChaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="248" mass="28066" checksum="9A7A04875822A795" modified="2011-11-16" version="1">MTNEGMWVIGYGSLIFKPPPHVQFKVTGYIKGFIRRFWQSSSDHRGTPQSPGRVVTLVSLSDLQTHTRFHNDLHMYELKGHAYEAECVSSGESDLDSIADISKKISDLSQDDLRVWGVAYYIGSEHVAEVKEYLDIREQDGYSTHKVPFHILNVEGGDKDKDIIDTIPKDPLTGDLFIESMIYIGTIENESFIGPESIEKTAKVIKTSHGPSGPNKEYLINLTEAVRHLDPKLRSHDYYLEDLVKLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASK8</accession>
    <name>G3ASK8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">2-(3-amino-3-carboxypropyl)histidine synthase subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_51906</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase, predominantly CBR1. Facilitates the reduction of the catalytic iron-sulfur cluster found in the DPH1 subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase. Facilitates the reduction of the catalytic iron-sulfur cluster found in the DPH1 subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the 2-(3-amino-3-carboxypropyl)histidine synthase complex composed of DPH1, DPH2, DPH3 and a NADH-dependent reductase, predominantly CBR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the DPH1/DPH2 family. DPH2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376727.1">
        <property type="nucleotide sequence ID" value="XM_007376665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASK8"/>
    <dbReference type="FunCoup" id="G3ASK8">
        <property type="interactions" value="980"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASK8"/>
    <dbReference type="GeneID" id="18871607"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51906"/>
    <dbReference type="eggNOG" id="KOG2648">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015210_1_1_1"/>
    <dbReference type="InParanoid" id="G3ASK8"/>
    <dbReference type="OMA" id="QIWNENH"/>
    <dbReference type="OrthoDB" id="449241at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120513">
        <property type="term" value="C:2-(3-amino-3-carboxypropyl)histidine synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11860:FF:000001">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010014">
        <property type="entry name" value="DHP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00272">
        <property type="entry name" value="DPH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF2">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01121">
        <property type="entry name" value="Diphthamide_biosynthesis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00408">
        <property type="entry name" value="Diphthamide_biosynthesis_famil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="435"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="435"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="449"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="563" mass="63165" checksum="B33C88BF76DD601B" modified="2011-11-16" version="1">MTEETVVAPSLSTYQDDSTFTFAKVSAKTDERKHLGEFHNEEELVKKIWQYYTLSDLVDFLKQKTEGETKYKRITLQFPDSLICDSAIIVQELQRELALEPCLSDDKSACDNLSGCCGKKDVDNGDKQRLWILADTSYSTCCIDEVAAEHVHSDLVVHFGDACLNDVEKLSAAYVFGKPEVDITSLVNSFKARYEDLNLPIVLMADAPHTYILHCLYEKLRSEYTKLIVADLHISKTSQATIIGYQPESSSSLHKFNRSFINLDADTSFESCDLFHITAPEAPRLLQLTTCFNSVSTYDPSTNLVSQGPFPNLMRRYKYMHVARSAGTIGLLVNTLSLTNTKKLINVIGARIKAAGKKHYVFVVGKPNVAKLANFESVDLWCVVGCDHQGIVLDQAGEYFKPIVTPYELLLALSDEMTWTGKWITDFASVLENYKETETESEEEKEKGENEDDDEDAPPEFDPVTGRYISTSRPLRQINHLMISSEEQNEEEDDDDTEKQLVQKFGSTIAIRNTVSTSAVHLQNRAWTGLGSDFTQESDNTGALVEEGRGGIARGYDFDRSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASL1</accession>
    <name>G3ASL1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_155826</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376730.1">
        <property type="nucleotide sequence ID" value="XM_007376668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASL1"/>
    <dbReference type="FunCoup" id="G3ASL1">
        <property type="interactions" value="598"/>
    </dbReference>
    <dbReference type="GeneID" id="18871031"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_155826"/>
    <dbReference type="eggNOG" id="KOG2164">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011811_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASL1"/>
    <dbReference type="OMA" id="QFDEQDF"/>
    <dbReference type="OrthoDB" id="302966at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039739">
        <property type="entry name" value="MAG2/RNF10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12983:SF9">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12983">
        <property type="entry name" value="RING FINGER 10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="163"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="18"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="562"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="466"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="577"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="587"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="617" mass="71193" checksum="FA651B20CA88F802" modified="2011-11-16" version="1">MTSSTNIVMAKDDSMVSNNSNIANTTSKSFTKTKNKNKPKNTQARMANPPVDLNFQIDTQIKQNKKKTFNKKNQISANHLLQFQSYRDSDEYHTNRSKPRKHNKLINEYQKPRIQLKGMKFINVNYKFVVDNRKQYRLQQLDPNVPFDIEDIIRIIVPRGNACPICLTDDPIAPRMITSCGHLICLKCVLLLLSSEVPKAKKRESEVIVEKYRECPLCSSVIRKHELKPVLINNVDEQFELPKVHHDTVLTLMKRQQDKIISLPKSMDNFDSSDYDSDFVDVAKLNEFLSYSRIFKGSSHYLLDMYNKEKDQILQAHNEEKLLYGSDDFSLVKATLDHIDDEIDHWTNKLAQDPPPAPTLIPSSTPTPPSDATYHYYQTGFNSSCTYVLSPLDMKVLKTTYGSYDNLPSSIVAKIENIKYEELSQETSLSKYKYLSHLPIGSQIGFLECNWFGNQYISHEAWQTFKEDLLKRTKNSQRKFKKEERDRKRAINEEEIRTRKFYEQENRDVERHESSGYSGVLGGVGALSIVDNRQTAEQPPLLSNSPTDVVIPEESFQTTVWGTKIPKPEPSASPVHEDDEDDWDAEEMIRRAKEEMSKQQGGKKKKKKMILLSSNNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASN5</accession>
    <name>G3ASN5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cargo-transport protein YPP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_142520</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in endocytosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic granule</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the YPP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376754.1">
        <property type="nucleotide sequence ID" value="XM_007376692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASN5"/>
    <dbReference type="FunCoup" id="G3ASN5">
        <property type="interactions" value="238"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASN5"/>
    <dbReference type="GeneID" id="18870455"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142520"/>
    <dbReference type="eggNOG" id="ENOG502QV6B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_312174_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASN5"/>
    <dbReference type="OMA" id="LIFIDYQ"/>
    <dbReference type="OrthoDB" id="29013at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23270">
        <property type="entry name" value="YPP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051722">
        <property type="entry name" value="Endocytosis_PI4K-reg_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23083:SF464">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT DOMAIN 7, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23083">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN, TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="890" mass="103509" checksum="57A52BFCFF744D1F" modified="2011-11-16" version="1">MIIDNSINEKYIRTLAFGCFPKNVLEFQESQDFFQQMIFIDYQKQLIIKEEHHNNDLFIPESKQKINQLIQYTNSITLNYENTEKSFEELLYYVVLLAHLYYLTGQVDFMTDVLNSISVPYSINEANLTSMTKNQIELVEYLHCKYHMLLGLVHENGFAIWLEYLSHFSKPFTKSQVIANHWLELLDLKLAESISQSITFDQVKRLSFFTNEISTIQFCNFLIRPENIDMADSQFKLEYTTFLVSQIEANIKRSVIFPNAMNSNDSTINNFINNLYESLSYVPFNLAIVKPTVSKRYLIDATSKTYQSKVVLSNLIYTLIDLNEYDEAFVAFKTYIEYLATEEQLSGHIDDILSIIDTFSTCIIHFNPIKSFKNEQKFKHTPDSMVEADLKVFTDKLLFYLNKLQEFIDLTYDESDTENDLSFLYRKYNPNILQTDNSQLIELIAKAWYSIGYYYSFVCMYDSVDETTMKQNVTKVLKNYKNSLIINSTGNILYLHSYALALANSQANKSALKLCKFILKKYPESFKTWNLLVLLLTSSEIDNPDTSKPDTTKLRDSEDFINKALNIAGLFIMKHQEKDIKLSFIEKTEILELKLTQLAVLESIYGLNYMMEYLNEVFILYHELFDVEFDTPARQQRDSARQFGSSARWSHRPSFIDPSNLPHKDVTKSRSPILDPLTTASNVVKAQTVNKLKRLSKIGRTQTRSSVVSTSRKSDNIKDRKLLQGLWLWTSRVFIRAGMLDEAEQCIVEAEAIYKPNIKTFTALGYLTSKTRKFLALQEFERSLEQVDKYNKQDLGWTLLGMAKLIFIDDITDNSLFISDKDLNGGIIRMKNLLENFTLSWPCGSNSSEVWYYLSKIYEIIDDKIMLDKSLWKCVELEDIRPVRSFSCDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASQ1</accession>
    <name>G3ASQ1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="14">ATP-PFK</shortName>
            <shortName evidence="14">Phosphofructokinase</shortName>
            <ecNumber evidence="14">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">PFK1</name>
        <name evidence="18" type="ORF">SPAPADRAFT_141651</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="14">Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 14 15">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="14"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377148.1">
        <property type="nucleotide sequence ID" value="XM_007377086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASQ1"/>
    <dbReference type="FunCoup" id="G3ASQ1">
        <property type="interactions" value="907"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASQ1"/>
    <dbReference type="GeneID" id="18870387"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141651"/>
    <dbReference type="eggNOG" id="KOG2440">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011053_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASQ1"/>
    <dbReference type="OMA" id="WHNLGGS"/>
    <dbReference type="OrthoDB" id="537915at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000007">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000008">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03184">
        <property type="entry name" value="Phosphofructokinase_I_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009161">
        <property type="entry name" value="6-Pfructokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040712">
        <property type="entry name" value="Pfk_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015912">
        <property type="entry name" value="Phosphofructokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02478">
        <property type="entry name" value="6PF1K_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF57">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18468">
        <property type="entry name" value="Pfk_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000533">
        <property type="entry name" value="ATP_PFK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 14 15" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="9 14 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase N-terminal" evidence="17">
        <location>
            <begin position="2"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="16">
        <location>
            <begin position="220"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="16">
        <location>
            <begin position="609"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal catalytic PFK domain 1" evidence="14">
        <location>
            <begin position="1"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal regulatory PFK domain 2" evidence="14">
        <location>
            <begin position="609"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="369"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="291"/>
            <end position="292"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="321"/>
            <end position="324"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="367"/>
            <end position="369"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="411"/>
            <end position="413"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="495"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="501"/>
            <end position="504"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <position position="678"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="735"/>
            <end position="739"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="773"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="780"/>
            <end position="782"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <position position="840"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="866"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="872"/>
            <end position="875"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <position position="949"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF18468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW31115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="978" mass="107650" checksum="FE302E4FC8D6AAA0" modified="2011-11-16" version="1">MEGLSFISLVTNSQDKFDNIARFYGKLGFRLTKNFSRVTSNGAEAAATNPKSHLGISKDSTREIWLESFPLQNLDKNGNIRPWQELEVYDGDSSEKLSTETTLKLRLSNVDNVDGNLPKKFIFFTTELSKVKAILESENIKYGQINETMILAEDPLTNTVCFSSTQNPLSRQSFTSAQEFIDTRTQEVLNNRKANTLASKASVISLDDTELVKPKIRKKKIAVMTSGGDAPGMNPAVRAVVRAGIYYGCDVFAVYEGYEGLVKGGDLLKKVEWADVRSYLSLGGTHIGTARCKEFRERSGRLEAAYNMVINGIDALVVCGGDGSLTGADLFRSEWPSLIEELVSTGRLSSKQVAPYKHLTIVGLVGSIDNDMSCTDVTIGAFSALERITEMVDYIGATAASHSRAFVVEVMGRHCGWLALMAGLATGADFIFIPERPPSAQNWKQELKDVCSRHRAYGRRKTTVIVAEGAIDDELNPITSEEVKNVLVELGLDTRITILGHVQRGGTAVAFDRRLATMQGVEAVRAVLEMTPNTPSPMIGVLKNKIVKYPLVDAVKQTKAVATAIDNKEFDKAMGLRDTSFYDSYRHFRSITYDDDGSKQLMDTDKRLNVAVIHVGAASAGLNAATRAVALYSISRGHNLYGVHDGFTGLCQGNLTKLEWLEVEGWHSKGGSELGTNRSLPSQDFGKVAYYLQKFNIQGLMIIGGFEAFTSLHELYSQRSHYPIFEIPMVVVPATVSNNVPGTEYSLGSDTCLNQLVSYCDAVQQSASSSRRRVFVVEVQGGHSGYVASYCGLITGALATYTPESKINLNALQGDLDLLFKVFASDRGEDHNGKLIIRNEQASSVFSTELIADILKENANGRFETRTAIPGHVQQGYTPTANDRVMAVRFALQAMEFIEDWNGCGVVHERSSDKKKSKHKHVVIGIHGDTIEFTSVHKLYETEANVELRKGKTIHWDDMIEVSDMLSGKLLLKKQRDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASW4</accession>
    <name>G3ASW4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NADPH:adrenodoxin oxidoreductase, mitochondrial</fullName>
            <ecNumber evidence="8">1.18.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_142046</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>2 reduced [adrenodoxin] + NADP(+) + H(+) = 2 oxidized [adrenodoxin] + NADPH</text>
            <dbReference type="Rhea" id="RHEA:42312"/>
            <dbReference type="Rhea" id="RHEA-COMP:9998"/>
            <dbReference type="Rhea" id="RHEA-COMP:9999"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.18.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8 9">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ferredoxin--NADP reductase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.18.1.6" evidence="8"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376779.1">
        <property type="nucleotide sequence ID" value="XM_007376717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASW4"/>
    <dbReference type="FunCoup" id="G3ASW4">
        <property type="interactions" value="571"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASW4"/>
    <dbReference type="GeneID" id="18870414"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142046"/>
    <dbReference type="eggNOG" id="KOG1800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024722_3_1_1"/>
    <dbReference type="InParanoid" id="G3ASW4"/>
    <dbReference type="OMA" id="RFNFIGN"/>
    <dbReference type="OrthoDB" id="333024at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004324">
        <property type="term" value="F:ferredoxin-NADP+ reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055275">
        <property type="entry name" value="Ferredox_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021163">
        <property type="entry name" value="Ferredox_Rdtase_adrenod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48467">
        <property type="entry name" value="GLUTAMATE SYNTHASE 1 [NADH], CHLOROPLASTIC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48467:SF1">
        <property type="entry name" value="GLUTAMATE SYNTHASE 1 [NADH], CHLOROPLASTIC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000362">
        <property type="entry name" value="FNR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0274">FAD</keyword>
    <keyword evidence="3 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 8 10" id="KW-0521">NADP</keyword>
    <keyword evidence="6 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="11">
        <location>
            <begin position="26"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="188"/>
            <end position="191"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="233"/>
            <end position="234"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="419"/>
            <end position="421"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000362-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000362-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW30746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="499" mass="55824" checksum="DA8B6012565624E5" modified="2011-11-16" version="1">MRGPTLIAKFGIRKFSYSTALLANSYKVAIIGTGPGGFYTAHHLLHKSSPDVKLHIDFFEKLPTPFGLSRYGVAPDHPEVKNCEEYMTNIMRDYSGGDKHSVRFLGNVEIGKDISLAELEKYYNSIVLAYGCTSADNKLNVPGADLPGVVAARQFVHWYNGHPDYYESGHKFVPPPLDKVKQVSIIGNGNVALDVARILLADPAEHWAKTDISTEAQELLAKSTVENVRIVARRGVLESAFSNKEIRELFELKNTSFIPLDETLLSSIDKSNLGRVDKRKLSIIEKYNKMSETPSQRKWSLEYLKSPVEFTASKTDPNLLESTTFVQNELVHDPLTQVTKCKATSTTITEPNELVIISIGYQGKALAGFDDLGIWFENNKLQNKAGRVLSIESRDNDDVNSVFKKGWYVAGWIKNGPKGVIATTMMDSFDTADKILEDISNKHVLPIEQTGDIIDALPKEIVTWDDWTKLDTYELQKGQELGKSRFKVCNKNDMLSHCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASX1</accession>
    <name>G3ASX1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_62606</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376786.1">
        <property type="nucleotide sequence ID" value="XM_007376724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASX1"/>
    <dbReference type="STRING" id="619300.G3ASX1"/>
    <dbReference type="GeneID" id="18874400"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62606"/>
    <dbReference type="eggNOG" id="KOG1453">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_509168_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASX1"/>
    <dbReference type="OrthoDB" id="19923at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF128">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN RGD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="337"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="194"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="196"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="216"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="535" mass="59429" checksum="3CF3C312471F32F1" modified="2011-11-16" version="1">MALATVTHSVTHLDQPPDQQQQQQLLQQQQLTRKTSLIRSLKHRRTSSNGSTSTPSSLNKFNFFKKDGVDSKTHSRKVSESSIHSLNGTAFSTPPLVNRNSFNGSFARADVIDEDKVPGFEFVNEPSNLGIAGIKAEISSLMSTKSELETGLITLKVERSQLEESLRKLSKQVTFEESKLNELHKQVAELQGTKARLTEENNQLREQNRHYKLTRSSSIETDSSSPLNKPTDLAESVTTLGESTTSNEDEQSHKATRLRFWRRQKNLVTTNAPVATIPESAQSSTSSVPSSFSAQGLRISQPVSNSVSGSKFHRSISTNILDSFLGDHQQSHDLFSSTIQARADYEGTKVPFIITKCISVVELRGLESEGIYRISGGNSTIVAIETAFANLAPGVTEDKKLEELMSGDINAVTSALKRYLRKLNDPIIPFGLYDEFIRVSTSNPRERRAQELKKVIYKLPRANRHTLWLLCNHLKLVSSMQNVNRMGFKNLAVVFAPTLARDSTGEREMTDMGARNDSTECLLTECEEVFAGYSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASZ2</accession>
    <name>G3ASZ2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DWNN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_62630</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376807.1">
        <property type="nucleotide sequence ID" value="XM_007376745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASZ2"/>
    <dbReference type="FunCoup" id="G3ASZ2">
        <property type="interactions" value="158"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASZ2"/>
    <dbReference type="GeneID" id="18874411"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62630"/>
    <dbReference type="eggNOG" id="KOG0314">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019105_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASZ2"/>
    <dbReference type="OMA" id="CGAKDHW"/>
    <dbReference type="OrthoDB" id="106784at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036002">
        <property type="term" value="F:pre-mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014891">
        <property type="entry name" value="DWNN_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033489">
        <property type="entry name" value="RBBP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025829">
        <property type="entry name" value="Zn_knuckle_CX2CX3GHX4C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439:SF0">
        <property type="entry name" value="CELL DIVISION CYCLE AND APOPTOSIS REGULATOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08783">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13696">
        <property type="entry name" value="zf-CCHC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01180">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51282">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DWNN" evidence="8">
        <location>
            <begin position="5"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="7">
        <location>
            <begin position="172"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW30774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="352" mass="40044" checksum="68AB582913A5E75E" modified="2011-11-16" version="1" fragment="single">MSSIIYYKFLHQKNQSVIHFDGTAISVFDLKKEIIQANQLGNGLDFNLTLYHSEQPDLEYEFDQDVIQRSSYVLVKRSPVSSNNKFNNASRYVTGKPRINRKMPTTAAPAVTTTTPIANTDNISEEDRIKLMFENQSNAWAQTQDELAQHKIIHYKPTTTGKEDAPPPGYICYRCGKKDHWIKNCPTNNDPNFEGKKIMRTTGIPKSYLKTISKQDIENKADSFSTNENGDVVDNDGNIILITDEGNYAVALADSKTWTSYQEKLANATKKSQQEFNDKLIKLIEQDNKKEFLDPLAEKKVLEPPIVMTPCCTESSKLQKMKNFNYNQGSLERVLIDNDFHCPNCGTEDTFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATD0</accession>
    <name>G3ATD0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">U3 snoRNP protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_62798</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UTP6 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376926.1">
        <property type="nucleotide sequence ID" value="XM_007376864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATD0"/>
    <dbReference type="FunCoup" id="G3ATD0">
        <property type="interactions" value="327"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATD0"/>
    <dbReference type="GeneID" id="18874469"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62798"/>
    <dbReference type="eggNOG" id="KOG2396">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026025_3_0_1"/>
    <dbReference type="InParanoid" id="G3ATD0"/>
    <dbReference type="OMA" id="YAKLEMI"/>
    <dbReference type="OrthoDB" id="28112at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013949">
        <property type="entry name" value="Utp6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055347">
        <property type="entry name" value="UTP6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23271">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN 66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23271:SF1">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08640">
        <property type="entry name" value="U3_assoc_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 6 N-terminal" evidence="6">
        <location>
            <begin position="9"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="432" mass="50281" checksum="0641011649F7407C" modified="2011-11-16" version="1">MAEKVRYYLEQSIPELEDLKRKGLFDKHEITMIMRRRTDFEHRIQGRGSKPRDFLKYVEFEGNLEKLRKKRYNRLNKVGLVETKPSISDWAGVRRIMFVFDRATRRYPGDTELWSHYLQFAKENGAIKVIYKVYSRLLQLQPRNVDAWLSAAKYEFEENANAKGARVLFQRGLRLNPESLELWLSYAQFELTYISKLLARRKVLGLITEKQQQEALESEQDKLAKSITESALGDDNDDFNADKIELPTAAEMKDELNHLPEADMNMLGNPETNPALKGDVALTIFDLCVPSILKTIPEYSTVINPQDKVFEIVERFLSLIDKFEDLNRDYLMLHVLNYVQKENPQDLRTLFIDITLPIRNVSRTSENLAEALQLSVNKFIAYKLKLKDASERTTLTNMFINKLNNQFLANTDNENEKVDALLKAIITKCRNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATW9</accession>
    <name>G3ATW9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tyrosine-protein phosphatase CDC14</fullName>
            <ecNumber evidence="4">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_157243</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="4"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377315.1">
        <property type="nucleotide sequence ID" value="XM_007377253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATW9"/>
    <dbReference type="FunCoup" id="G3ATW9">
        <property type="interactions" value="831"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATW9"/>
    <dbReference type="GeneID" id="18871070"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157243"/>
    <dbReference type="eggNOG" id="KOG1720">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017787_1_2_1"/>
    <dbReference type="InParanoid" id="G3ATW9"/>
    <dbReference type="OMA" id="ACMLCCY"/>
    <dbReference type="OrthoDB" id="5632at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120105">
        <property type="term" value="C:mitotic actomyosin contractile ring, intermediate layer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071958">
        <property type="term" value="C:new mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140602">
        <property type="term" value="C:nucleolar peripheral inclusion body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030869">
        <property type="term" value="C:RENT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071470">
        <property type="term" value="P:cellular response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140013">
        <property type="term" value="P:meiotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051229">
        <property type="term" value="P:meiotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044878">
        <property type="term" value="P:mitotic cytokinesis checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016479">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000786">
        <property type="term" value="P:positive regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031536">
        <property type="term" value="P:positive regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903501">
        <property type="term" value="P:positive regulation of mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140429">
        <property type="term" value="P:positive regulation of mitotic sister chromatid biorientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902846">
        <property type="term" value="P:positive regulation of mitotic spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031031">
        <property type="term" value="P:positive regulation of septation initiation signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070550">
        <property type="term" value="P:rDNA chromatin condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14499">
        <property type="entry name" value="CDC14_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17657">
        <property type="entry name" value="CDC14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000038">
        <property type="entry name" value="Tyrosine-protein phosphatase CDC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000073">
        <property type="entry name" value="Tyrosine-protein phosphatase CDC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044506">
        <property type="entry name" value="CDC14_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029260">
        <property type="entry name" value="DSPn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14671">
        <property type="entry name" value="DSPn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0469">Meiosis</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="16">
        <location>
            <begin position="177"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="17">
        <location>
            <begin position="255"/>
            <end position="320"/>
        </location>
    </feature>
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        <location>
            <begin position="481"/>
            <end position="552"/>
        </location>
    </feature>
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        <location>
            <begin position="486"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="523"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069949"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EGW30344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="552" mass="62796" checksum="9C47D624F4D4C82C" modified="2011-11-16" version="1">MGKNIVDVPLIEFLKNRIYLGAFDYSPPNTKDLVYFTIEDSLPYNSFHLDFGPLNIAILYRFAVILHNILNEENNQGKAVVFYSSSCPKQRANAACLLCCYMVLIQSWSPHQVLQPIAQCDPPFQGFRDAGYSHADYEITIQDIVYAMWRAKERGMIDLTSFNLEEYEQYERVDQGDFNVISKDFIAFASPQQTKRNVINEPFRKVLSYFMENNVQLVVRLNSHLYDSQEFTKRNIRHIDMIFDDGTCPTLDYVQKFVGAAETIINQGGKIAVHCKAGLGRTGCLIGAHLIYTHGFTANECIAYMRMLRPGMVVGPQQHWLYLHQNDFRDWRHTMVLDNRPCDFIDGLYPLISYEEFKTRMKIEKKKLREQEANALTVNSSFSQMDSSFQTPIRRRKISGALTSKIQLAVPNDSPGQPRKYMEAAEEHETELINNSDDDDDDENVVMMQDIQSTPGKDGVTTSTPNQWKILRSISTNARRTNQPLSVTKTTTTTTTRTVSHTLSSSPAGSPSIRMPKARSSKNRVISANGIQSQTQVRVHSGGIRKVSGKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="140"/>
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        <location>
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    </feature>
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        <location>
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            <end position="251"/>
        </location>
    </feature>
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            <begin position="237"/>
            <end position="249"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EGW30390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="268" mass="29853" checksum="27D511F57F1DF69E" modified="2011-11-16" version="1">MRVIHTFLNQFFLVGATLLLVLTVISGTTNNSSLRNFYWLRADVAGIQNAPADTSVWTFWGVCDKSNFTNCILGPAFPISPTDNFGTTENIPTDFIDNRNTYFYLSRFAFAFAIIALAFTGLAVIVEFLAYCFELIDRVVIFLITFGVFFMAGFASMQTAVVVLARNAFRNGGRTSNVNATLLGVTWAAFACLVICWFLTFAANIAQSYRKHIDRVHEREAAQAPIADDSSFTRAAPPDEPKDTEDHTGGIRFFKIKRNQKVSDEESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU23</accession>
    <name>G3AU23_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
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            <taxon>Dikarya</taxon>
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            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007377308.1"/>
    </dbReference>
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    <dbReference type="STRING" id="619300.G3AU23"/>
    <dbReference type="GeneID" id="18872101"/>
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    <dbReference type="eggNOG" id="ENOG502S52I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="OMA" id="SYCFWPF"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF80">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
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        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="211" mass="23959" checksum="ED48808745CCC59E" modified="2011-11-16" version="1">MSLPLSEKRNDLEKSSLSPISSASSSSHLHPAPKRVYQKLISALIIETIISCAIYINYDTIGAYHPMLAGLLLGCSSGALAQSMNQYAKKKFTLQKILKFMLWGSINGYFTVGWINILITRIDNSAYRIIIDQLVGAPMFQLIFNMLNSIWDHGEMFSISTRLAYVKSLKYSYCFWPFFSILEFMFIPQALMFPCNCLANLLWNLILCRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUD7</accession>
    <name>G3AUD7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_73218</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377483.1">
        <property type="nucleotide sequence ID" value="XM_007377421.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="spaa:SPAPADRAFT_73218"/>
    <dbReference type="HOGENOM" id="CLU_1343994_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUD7"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="204" mass="23303" checksum="76785C7BF6654E2E" modified="2011-11-16" version="1">MISCEYISHMLLNSSIEDMKFEIVYCVLLLHTDTVNRLLILLLVLNLLQSLRFSETQLRHLFSIHLTIVHSTIQDTFVHLNVLDILIPLSGIQSINHSPHIFQVQQKIVGISRTQSKCHIISPGFLVVFPVGNPVGNDNIQILLITKPKFFTFTSNWNIKPKRTRHVRDFELGSLTTEYEKVGTVYFLTASTLLQMGTHKPNPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUL0</accession>
    <name>G3AUL0_SPAPN</name>
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        <recommendedName>
            <fullName evidence="4">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_63402</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377537.1">
        <property type="nucleotide sequence ID" value="XM_007377475.1"/>
    </dbReference>
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        <property type="interactions" value="788"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUL0"/>
    <dbReference type="GeneID" id="18874733"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63402"/>
    <dbReference type="eggNOG" id="KOG2190">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_877624_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUL0"/>
    <dbReference type="OrthoDB" id="1937934at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="4"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="226"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="112"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="317" mass="34790" checksum="DC331D3C76061640" modified="2011-11-16" version="1">MMSQQYNLKLLVPHPMIGFIIGKQGLKFREIEESSAAKLRAAEQPLPYSTDRVLSVTGVADAIHIAVYYIAQVMIEHKDCLKKNKIVYYNPSNYHHGNISSNMMALGGANNMQQPPPQQHRNDPYGMPYGQQSMPKPPQQSASPYNFQTMFQPAIYPQQHQPQLQPQTQYGMSSSYGNMGMNMGGAAGGMGGASIPPQNQYIDEHGNTIVGDVITNPPIQAGPSPDKFNQDVFVANANIGSVIGKGGNNIKHIRENSGCSYVKIEPDRGQSIMLGGGKGLTNIRKLTLTGSLNSIQMAIYLINQRINQDKERNAPHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUN1</accession>
    <name>G3AUN1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MoaB/Mog domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63429</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377558.1">
        <property type="nucleotide sequence ID" value="XM_007377496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUN1"/>
    <dbReference type="FunCoup" id="G3AUN1">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUN1"/>
    <dbReference type="GeneID" id="18874745"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63429"/>
    <dbReference type="eggNOG" id="KOG2644">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030805_0_2_1"/>
    <dbReference type="InParanoid" id="G3AUN1"/>
    <dbReference type="OMA" id="MARMPRG"/>
    <dbReference type="OrthoDB" id="448496at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047884">
        <property type="term" value="F:FAD diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042726">
        <property type="term" value="P:flavin-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47675">
        <property type="entry name" value="MOLYBDOPTERIN BINDING DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G11210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47675:SF1">
        <property type="entry name" value="MOLYBDOPTERIN BINDING DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G11210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="1">
        <location>
            <begin position="15"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="194" mass="22205" checksum="6620F755CED03A6A" modified="2011-11-16" version="1" fragment="single">MAQYSQQTKPIRTAGCLIIGDEILNGKILDTNSYNFARYCFNNLSIPLKRTIVCGDSEKDIIDSLNILLKQDKLDFVVTSGGLGSTHDDITYKVISDYFNLDYGIDQEVVDRMHRVRGDHLDKLKPDQLSAFYKMATLPIEKNQSSTKVEKYFVGEDLWFPLVALNEQVYVLPGIPQLFTRLINEMEPYLKSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUQ8</accession>
    <name>G3AUQ8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_68687</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377585.1">
        <property type="nucleotide sequence ID" value="XM_007377523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUQ8"/>
    <dbReference type="GeneID" id="18875235"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68687"/>
    <dbReference type="HOGENOM" id="CLU_937405_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUQ8"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="52"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="175"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="297" mass="34863" checksum="2C88453478844825" modified="2011-11-16" version="1">MLRASKSIQQASKLRSWSSDITTSVSDKQLPNRDNAMISVANSSDAENLNTSSEVESDLDKLRIENQKLKLQNSKLLQDLKDNNIELTNLKETLKLHVKLGQEKDNKISRLKAEITMQKDCLNWETYQIKKLRAEVTNIRGTFRVKDLLIQSLTNQWESCKKSSEKKLEEFGKKVNTQEKVIKKFKDKIKELYKTVDEQEDKIEEQEETFGRIIDEITSANLTWALMCYSLFQLNLSFFQNFLTYESIHEYNSILDNFHFPSKLDPEEMEWIKNLFLRNEEMFESLTGSIWRDIPNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV14</accession>
    <name>G3AV14_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_63713</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377855.1">
        <property type="nucleotide sequence ID" value="XM_007377793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV14"/>
    <dbReference type="STRING" id="619300.G3AV14"/>
    <dbReference type="GeneID" id="18874879"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63713"/>
    <dbReference type="eggNOG" id="KOG4409">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017361_3_1_1"/>
    <dbReference type="InParanoid" id="G3AV14"/>
    <dbReference type="OMA" id="ADQHLVM"/>
    <dbReference type="OrthoDB" id="4016300at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035965">
        <property type="term" value="P:cardiolipin acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF23">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE ICT1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="123"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="302"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="491" mass="55800" checksum="C394A21221DF3F93" modified="2011-11-16" version="1">MTTQTEAQPNLPDQHPDAMSRMRNKKNEFNDGRKRPIYSWKDSAFDWIKQTVYSDYSDEKVEANLLSHLPFFPESDGKRRASVINTDLGDGKYIHELFIENIEPVTESSAVMNPNGLPATKEVVLVHGYAASLGLFIDNFDSLSSIPGIKIHAIDLLGFGFSARPNFPHFPSKTKQDIYKVEDWFIDSIEEWRKKRNINNFVLIGHSFGGYLSCAYTLKYNQKLIDAATGINHNLVEKLILLSPVGVERHKNSLLKDQESVSISVAQQQKENQLSPVVSLEQEVLTSQEEIVHGKAGVKPLSASPSASVSQSSNQEASEEEPRTRRRKIIDFMWERNFSPFSIVRNLGPIKSKMISGWTTHRFAHVYYQDQQHFQNVHDYMYRIFNGKGSGEYALTRVLGVGALAKLPLLDRCPEKFVAMGIPTLWVYGDKDWMNDEAGLEMVNEINELSIKKSEKKLASFSILPNAGHHLYLDNPPAFAREIFKFLGFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV39</accession>
    <name>G3AV39_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Double-strand break repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">SPAPADRAFT_144348</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. Within the MRN complex, MRE11 possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. MRE11 first endonucleolytically cleaves the 5' strand at DNA DSB ends to prevent non-homologous end joining (NHEJ) and licence HR. It then generates a single-stranded DNA gap via 3' to 5' exonucleolytic degradation, which is required for single-strand invasion and recombination.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16 18">Belongs to the MRE11/RAD32 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377879.1">
        <property type="nucleotide sequence ID" value="XM_007377817.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV39"/>
    <dbReference type="FunCoup" id="G3AV39">
        <property type="interactions" value="1027"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV39"/>
    <dbReference type="GeneID" id="18870618"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144348"/>
    <dbReference type="eggNOG" id="KOG2310">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009535_3_0_1"/>
    <dbReference type="InParanoid" id="G3AV39"/>
    <dbReference type="OMA" id="QNHTGHT"/>
    <dbReference type="OrthoDB" id="30417at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140445">
        <property type="term" value="C:chromosome, telomeric repeat region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030870">
        <property type="term" value="C:Mre11 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051880">
        <property type="term" value="F:G-quadruplex DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030437">
        <property type="term" value="P:ascospore formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010791">
        <property type="term" value="P:DNA double-strand break processing involved in repair via synthesis-dependent strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990918">
        <property type="term" value="P:double-strand break repair involved in meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035753">
        <property type="term" value="P:maintenance of DNA trinucleotide repeats"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010780">
        <property type="term" value="P:meiotic DNA double-strand break formation involved in reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097552">
        <property type="term" value="P:mitochondrial double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062176">
        <property type="term" value="P:R-loop processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000011">
        <property type="entry name" value="Double-strand break repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.110">
        <property type="entry name" value="Mre11, capping domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003701">
        <property type="entry name" value="Mre11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038487">
        <property type="entry name" value="Mre11_capping_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007281">
        <property type="entry name" value="Mre11_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00583">
        <property type="entry name" value="mre11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10139">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR PROTEIN MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10139:SF1">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR PROTEIN MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04152">
        <property type="entry name" value="Mre11_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000882">
        <property type="entry name" value="DSB_repair_MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01347">
        <property type="entry name" value="Mre11_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 16 18" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 16 18" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 16 18" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11 16 18" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="10 16 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 16 18" id="KW-0464">Manganese</keyword>
    <keyword evidence="15 16 18" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 16 18" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14 16 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mre11 DNA-binding" evidence="20">
        <location>
            <begin position="290"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="537"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="538"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="554"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="17">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000882-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM01347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EGW30113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="577" mass="65081" checksum="0E6477E747A8C129" modified="2011-11-16" version="1">MLVDNLPQDEHTIRILLTTDNHVGYAESDPIRTSDSHLTFHEITQIASTNNVDMIVQGGDLFHITKPSKKSMYHVMQSLRLNCMGDKPVEIEMLSDPEVLGSFGVNYEDPNVNVSIPVFAISGNHDDATGEGLLSAMDVLAMSGLINHFGAIPDSENITVSPLLFQKGTTKLALYGMANVRDERLHRAFRDGIVKFLRPDIHTDEWFNFLAFHQNHATHSFTSSIPESFLPRFLDFILWGHEHECVPYPVHNPEMEFEVLQAGSSVATSLSEGEVPDKHIFIMSVRGQDYSIEPIKLTTVRPFILKVLELSKTDLIPGAASKSDVIEYLTSEMELAIEAANTKWKESNPDRTDTPPLPLIRLKVEYSGGYEIENVKRFSNRFVGKIANANDAIQFFKKRQPREQTLNRTKFNAIEENTMNKKSTELQLHDIINDFLKQTQLTLIPEVGLNDAVKRFIENEDKTSLSQYIEKEVKRETKMLLSVDIDEDQFHNGDDVKAKNAFKQLLKEIAPSASIPVEIDIPDIPVPAQEEVHHILDSESDVEILSDDDCAPKAKSRAKPKTRAPAKSRTTRTRNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVB5</accession>
    <name>G3AVB5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_52961</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377900.1">
        <property type="nucleotide sequence ID" value="XM_007377838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVB5"/>
    <dbReference type="GeneID" id="18871743"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52961"/>
    <dbReference type="eggNOG" id="ENOG502QQDI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_279288_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVB5"/>
    <dbReference type="OMA" id="VELMYED"/>
    <dbReference type="OrthoDB" id="4096741at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045112">
        <property type="entry name" value="PPAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12661">
        <property type="entry name" value="PETER PAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12661:SF5">
        <property type="entry name" value="SUPPRESSOR OF SWI4 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="648"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="952"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="436"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="648"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="740"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="790"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="802"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="840"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="875"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="959"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="971"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1041"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1075"/>
            <end position="1096"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1129" mass="127555" checksum="506B084352ABA685" modified="2011-11-16" version="1">MSKYAPETNNRSLPQSPSSSGLAAATAALRMHSSPVSSSPVKQPPVQRHPAAAPRSNSLSNRSNSLRQYTYHPKASYAVGQPSIPNPRRYSSLTNKVNSTYAQFAPPEDDEDMEETVVTTTTTKVVDSHGRTQSITTKTVTTLPDGSNIIETTTKNISRTSSRANSLNSGANLRVNSLTHSNNLEKIDEQLQNFEYDYQVDSSHLDSPDTMLKLNLPDNANQSTPKEILPVHSDEPKPIAKTDSVEKPRSILKNSQRPPMEEPYDQPLNDVSPASPRSLSTPPNSITFNEKVKTIPVYTEHKTLRKASKPRPQSHVPPPSSPKTPVPDADFYAAAMQAAYKKVYGDRVPDAPVATAAPLTPPPPAVPAAAVDEVEPASPKKSKFGFLPLSPRRMSISKEKPNIPVPTTIIPDHKHEGEIPDDYKYQGHHRQFATHSMRDNIAKKDTSRKERLKEEKRQAKEEADRQKREAKERAAQEKKEAKERAILEKKLKKEQEEENKRLAKEKAEEEKQLAKEKAEEEKRLAEEEKILAKERAEEEKRLAKEKAEEEKRLAKEKAEEEKRSAEEERRIAKERAEEEKRLANEKAVEAKRLAKQQEEEERRIVKEKELEQKRIAEEKELEERRLAQEKELEERRLAEKEIEERRLAKEKEEEEIRIAKQREHEEQKMAEEQKMAEEQKQLAIEKEREQIRLAKEKQAEEKRLTREQAEEESRLAREQSEERKQLAEQRSLAQEVKEDEENKIAQAEEPRLSDQDIEEEMPRAQEKTDAEKHLAAENTPGEQPRATEILPDKTVSRENSTKESVGNSRTETHNQTSQPTTPKKRGSKIFNIFGRHKKSASTASSHFSHRTISNPNNMESETIKLTEEASSIQKYESKDATRANHEVQSNRDTVDNPTIKSDNHEYVSGVSSFAEFVDVHITNDHDDASSIAESPAVMDEVKRQSTIDDVIAPEQSELEAPKLDPAKTHDTVSSTQETSRPFLTPAGTLDDVKLQPTNESQVENAIEHEDIGTLTKETTDEDVGGVSSPQLTEVEPPLEKTTPQVTAQPQQSSLTDLQPPAEPKLKNEAPIASADDNSTPVSEQESTQGTHSTNATDHQDLDDTEVTTKKKKPIGKFRRTVYKYFVNKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVK5</accession>
    <name>G3AVK5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Gamma-butyrobetaine dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_57449</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the gamma-BBH/TMLD family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377720.1">
        <property type="nucleotide sequence ID" value="XM_007377658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVK5"/>
    <dbReference type="FunCoup" id="G3AVK5">
        <property type="interactions" value="663"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVK5"/>
    <dbReference type="GeneID" id="18872121"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57449"/>
    <dbReference type="eggNOG" id="KOG3888">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021859_0_1_1"/>
    <dbReference type="InParanoid" id="G3AVK5"/>
    <dbReference type="OMA" id="VHITWPN"/>
    <dbReference type="OrthoDB" id="406634at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045329">
        <property type="term" value="P:carnitine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00250">
        <property type="entry name" value="CAS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2020.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050411">
        <property type="entry name" value="AlphaKG_dependent_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038492">
        <property type="entry name" value="GBBH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696">
        <property type="entry name" value="GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696:SF25">
        <property type="entry name" value="OXIDOREDUCTASE AIM17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="7">
        <location>
            <begin position="151"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW29954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="441" mass="50894" checksum="832457D93969A5B5" modified="2011-11-16" version="1">MLRRFPVNQLKRCSLNHIRLASSLSLVEYSDDQISIMLNHHLLHFKTAFLRDACQDPSSVDPYNKQKLFSTTEAVTGLKVEAPPKVIEDSVTKELKLCVDWNTNGVTKSSEYPISLLEKYSTKASRRAGKFFDKERKLWDRQEIESNLSQLEVDYADFISNQDALSKTLHNLNKYGLTFVNNIPEPQLESMNEDNAYEWPVAKLASRFGYIKKTFYGTLFDVKNENTDAKNIANTNVYLPLHMDLLYYESPPGLQLLHFIKNSTIGGESVFCDSYLAAQRVKELDPEAYKALTEIPITFHYDNNNEYYYYRRPLIIEDPDIAAVTKDFPPIDVVNYAPPFQGPLEVGISTREDSSVEEAKQYHNFDDFLRGLEIFEKTINEPENHFEIKMKEGSCVIFENRRALHSRKSFSDENGGDRWLMGTYVDGDSFRSKLRVSKRND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVM5</accession>
    <name>G3AVM5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_63803</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377956.1">
        <property type="nucleotide sequence ID" value="XM_007377894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVM5"/>
    <dbReference type="FunCoup" id="G3AVM5">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="GeneID" id="18874921"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63803"/>
    <dbReference type="eggNOG" id="KOG0255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008455_11_0_1"/>
    <dbReference type="InParanoid" id="G3AVM5"/>
    <dbReference type="OMA" id="MNTFILE"/>
    <dbReference type="OrthoDB" id="9986881at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033101">
        <property type="term" value="C:cellular bud membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000297">
        <property type="term" value="F:spermine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015847">
        <property type="term" value="P:putrescine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF31">
        <property type="entry name" value="POLYAMINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="303"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="374" mass="41641" checksum="D54B69D93839426B" modified="2011-11-16" version="1">MVISCLGYVCFSFAVATAKDLQTIMICRFFAGFIGAAPLVVAPAVMADLFSAKIRGTAISIFAMILFGGPMLAPIIGGFTVKNPDMGWRWTSYLCGIIGCAALVMNTFILEETHHPIILVRKAEMLRRRTGNWGIYAPHEELTLSLKEIVEKNIARPMKLLFTEPIILLISIYNAFIYGMLYMFLTAIPLIFSVNYRFSPGVAELPYISMLIGVFTGGLIIIYFERRYNIAMDNNGGRPLAEERLPPMMIGSVTFVIGIFWLGWTGSYPEHVHWIVPTIGAAFVGNGLILIFLPCMNYIIDCYLYLAASALAGNTFLRSSFGAAFPLFSMQMFVNMKIQWASTLVGCIGVAMIPVPFLFYKYGKKIREKSKFAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEB4</accession>
    <name>G3AEB4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Saccharopine dehydrogenase [NAD(+), L-lysine-forming]</fullName>
            <shortName evidence="13">SDH</shortName>
            <ecNumber evidence="4 13">1.5.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 13">Lysine--2-oxoglutarate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LYS1</name>
        <name evidence="19" type="ORF">SPAPADRAFT_147229</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>L-saccharopine + NAD(+) + H2O = L-lysine + 2-oxoglutarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:57951"/>
            <dbReference type="EC" id="1.5.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the AlaDH/PNT family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.7" evidence="4 13"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373114.1">
        <property type="nucleotide sequence ID" value="XM_007373052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEB4"/>
    <dbReference type="FunCoup" id="G3AEB4">
        <property type="interactions" value="200"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEB4"/>
    <dbReference type="GeneID" id="18870743"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_147229"/>
    <dbReference type="eggNOG" id="KOG0172">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063085_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEB4"/>
    <dbReference type="OMA" id="YFFFSHT"/>
    <dbReference type="OrthoDB" id="265306at2759"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00034"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004754">
        <property type="term" value="F:saccharopine dehydrogenase (NAD+, L-lysine-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12188">
        <property type="entry name" value="SDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000217">
        <property type="entry name" value="Saccharopine dehydrogenase [NAD(+), L-lysine-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000423">
        <property type="entry name" value="Saccharopine dehydrogenase [NAD(+), L-lysine-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051168">
        <property type="entry name" value="AASS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007886">
        <property type="entry name" value="AlaDH/PNT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007698">
        <property type="entry name" value="AlaDH/PNT_NAD(H)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027281">
        <property type="entry name" value="Lys1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133:SF23">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE [NAD(+), L-LYSINE-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05222">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018250">
        <property type="entry name" value="Saccharopine_DH_Lys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01002">
        <property type="entry name" value="AlaDh_PNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01003">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 13" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="8 13 15" id="KW-0520">NAD</keyword>
    <keyword evidence="7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase N-terminal" evidence="18">
        <location>
            <begin position="8"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase NAD(H)-binding" evidence="17">
        <location>
            <begin position="175"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="14">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="204"/>
            <end position="205"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="319"/>
            <end position="322"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="206"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR018250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR018250-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR018250-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR018250-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW35702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="368" mass="40701" checksum="712FAED2F728B756" modified="2011-11-16" version="1">MSQPVTIHLRAETKPLEHRAALTPTTTKQLLDAGFKVYVEESSQSIFQTKEYEEVGATIVPEGSWKSAPADRIILGLKELPEETFPLIHEHVQFAHCYKDQGGWKDVLSRFPAGKGTLYDLEFLENDQGRRVAAFGYYAGFAGAAIGVLDWAFKQTHSDNENLPGVQPYPNEDALISDVKKQLDLALAKNGGAYPTTLVIGALGRCGSGAIDFFKKVGIPEANIAKWDMAETAKGGPFQEIVQSDIFVNCIYLSQPIPPFIDFSSLNVEDRRLRTIVDVSADTTNPHNPIPVYTIATVFNDPTVPVETTKGPKLSVCSIDHLPSLLPREASEFFSRDLMPSLLELPNRHTAPVWVRAKQLFDKHVARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AED9</accession>
    <name>G3AED9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein FAF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_58924</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373139.1">
        <property type="nucleotide sequence ID" value="XM_007373077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AED9"/>
    <dbReference type="FunCoup" id="G3AED9">
        <property type="interactions" value="227"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AED9"/>
    <dbReference type="GeneID" id="18872682"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58924"/>
    <dbReference type="eggNOG" id="ENOG502QVP1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069402_0_0_1"/>
    <dbReference type="InParanoid" id="G3AED9"/>
    <dbReference type="OMA" id="FEAQFKP"/>
    <dbReference type="OrthoDB" id="5556956at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053030">
        <property type="entry name" value="Ribosomal_biogenesis_FAF1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28096">
        <property type="entry name" value="PROTEIN FAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28096:SF1">
        <property type="entry name" value="PROTEIN FAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="21"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="254"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="123"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="36"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="274"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="284" mass="31988" checksum="D0B318F4316F5093" modified="2011-11-16" version="1" fragment="single">MSDDDYRRALEIQRRNFEAQFGSIEELGFEDKSKSVHASSSDDNSSGIESENESGSDEEQYNFKGFESSGESDSPDFESEEEDDDEEEEEAPKVIKLNATIAKPTIISKQDRKLLKSGRAPTLQELELKSQQASKQTKAQIKEDSENLENDLKLQRLLQESHILTHSLEHSGAELTLETIDYEDPTGKARRRILDSRMRQLAATNSNENRKLESMPMSMRKGMIASRDKKIKKFEADARDAGIVLSKVRKGQVRDLNAGKGSTLTSDRLGSGVNKKKHHRDRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEE5</accession>
    <name>G3AEE5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_147308</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373144.1">
        <property type="nucleotide sequence ID" value="XM_007373082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEE5"/>
    <dbReference type="FunCoup" id="G3AEE5">
        <property type="interactions" value="394"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEE5"/>
    <dbReference type="GeneID" id="18870747"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_147308"/>
    <dbReference type="eggNOG" id="KOG0039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010365_4_0_1"/>
    <dbReference type="InParanoid" id="G3AEE5"/>
    <dbReference type="OMA" id="GWSYRTF"/>
    <dbReference type="OrthoDB" id="4494341at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000293">
        <property type="term" value="F:ferric-chelate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF9">
        <property type="entry name" value="FERRIC REDUCTASE TRANSMEMBRANE COMPONENT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442232" description="FAD-binding FR-type domain-containing protein" evidence="15">
        <location>
            <begin position="20"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="255"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="368"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="16">
        <location>
            <begin position="444"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="119"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW35732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="733" mass="81388" checksum="CCDFC9EF75FEE9A6" modified="2011-11-16" version="1">MKVSTLISLLITSSALVSAVKTYSELDLALMACEGYFTVNMAVCQAGKKTNKDMDCYCNSDPGFGTLADCFVKGYSNDTSLIKSFAKSCNSTVDKFYTKYDSMKLKKFEMPKPMAQGMGGDGGKGGKGGKGGKGGKGGKGGGGKGDQAAVELSKVPLALDYSKFTPYREAYEMTYNNYNLGLTHGAALIGYWGIVIVIATVSNFFTKLFPSALSGTIPTLYRKYITMPATFHKKKTNSYHFGPIAGLIPARLESFYVFAFVFLAALLAGIDIKHVENNPLNPIRQVEMGHLVADRTGIMACFLVPLLFLFGGRNNFLQWITGWKFGTCIMYHRWISRVAAILIMVHGISFSASDLAAGRYSTRMSQAFMQWGVVACICAGFTIVQSLLFFRRKCYEVFLVIHILLAVFFSIGAWYHLTDQGYAEFMYAAVAVWAFDKFIRLCRLASFGVQKAEITLLANETLKIIVPKPSYWKSIPGGHSFVTFLLPSCFWQQHPFTFTTSHESEGKIVFYTKVKNGITKKLYKKLSKCNGLTTTVNVMIEGPYGKPSDGRRAKNVVYVAGGNGIPGIYSECLDVAQNSSSSQSIKLIWVIRHWKSLSWFIDELKKLENTKVQTTIYVTRPDITEGVEHILKGRSEKEKEKSEAEKSSDVSVVSYPASTMSSLRQELSHVTFVEGRPSMDAHVEEEIKQANGSLAFVTCGHPVMVDDLRYAVTHHLQDTTHRVDFYEQLQTWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEH6</accession>
    <name>G3AEH6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosome biogenesis regulatory protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_57806</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in ribosomal large subunit assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the RRS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372150.1">
        <property type="nucleotide sequence ID" value="XM_007372088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEH6"/>
    <dbReference type="FunCoup" id="G3AEH6">
        <property type="interactions" value="602"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEH6"/>
    <dbReference type="GeneID" id="18872202"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57806"/>
    <dbReference type="eggNOG" id="KOG1765">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065163_2_1_1"/>
    <dbReference type="InParanoid" id="G3AEH6"/>
    <dbReference type="OMA" id="KMVYDEA"/>
    <dbReference type="OrthoDB" id="28455at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007023">
        <property type="entry name" value="Ribosom_reg"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04939">
        <property type="entry name" value="RRS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="203" mass="23014" checksum="FC96DB2704F8BE19" modified="2011-11-16" version="1">MSNETEYKPVTVDKPIPNTYDLGNLATFDPNPLDNSKLTSDSDREEYLTSVTRDNVQLLINQILSLPVKTTTDTHGSSTGQNSTMTLIQLPEPSTQLPREKAIPKPKPPTKWEQFAARKGIKAKAKDGKMVYDEATGEWVPKWGYKGKNKELDDQWLVEVDDKVKDTPDELIDPRTLSRAERKKLVKKNELQHKRNLKNQGAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEI6</accession>
    <name>G3AEI6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-dehydropantolactone reductase</fullName>
            <ecNumber evidence="6">1.1.1.358</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2-dehydropantolactone reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Ketopantoyl-lactone reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_132075</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(R)-pantolactone + NADP(+) = 2-dehydropantolactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16719"/>
            <dbReference type="ChEBI" id="CHEBI:18395"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.358"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>isatin + NADPH + H(+) = 3-hydroxyindolin-2-one + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:68608"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27539"/>
            <dbReference type="ChEBI" id="CHEBI:28536"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.358" evidence="6"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372160.1">
        <property type="nucleotide sequence ID" value="XM_007372098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEI6"/>
    <dbReference type="FunCoup" id="G3AEI6">
        <property type="interactions" value="210"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEI6"/>
    <dbReference type="GeneID" id="18869634"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132075"/>
    <dbReference type="eggNOG" id="KOG1577">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_0_3_1"/>
    <dbReference type="InParanoid" id="G3AEI6"/>
    <dbReference type="OMA" id="AWKAMEG"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047011">
        <property type="term" value="F:2-dehydropantolactone reductase (A-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004032">
        <property type="term" value="F:aldose reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051268">
        <property type="term" value="F:alpha-keto amide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051269">
        <property type="term" value="F:alpha-ketoester reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016652">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042180">
        <property type="term" value="P:ketone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19120">
        <property type="entry name" value="AKR_AKR3C2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044494">
        <property type="entry name" value="AKR3C2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF3">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="12">
        <location>
            <begin position="33"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="11">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00079693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00081322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="303" mass="33727" checksum="6CFC8815AFCC62D3" modified="2011-11-16" version="1">MSLVGTDFKLSNGNTIPAIGYGVGTKWFKFGRDEVDTNVINALETAIKLGFAHIDGAEIYNTNEEIGAALVNSGVKRENIFITDKYFAGDASFSNRSPHSNPYEALKADLKQLKLDYVDLYLLHSSYIKKETHGFSLTEAWGYLEKLKDEGLAKNIGVSNFTVEDLKTVLDSKPKYKPEVNQIEFSAYLQDQTPGIVQYSQENGILVEAYGPLGPITKGQGGPLDPVLAKLSKKYGKTEGQILLRWVIQKGVLPLTTSSKEDRIKSFVDIFSFELTPEEVDEITTVGKQKTVRQFAKEFSKYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEM9</accession>
    <name>G3AEM9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BioF2-like acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_69822</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373067.1">
        <property type="nucleotide sequence ID" value="XM_007373005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEM9"/>
    <dbReference type="STRING" id="619300.G3AEM9"/>
    <dbReference type="GeneID" id="18875294"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_69822"/>
    <dbReference type="eggNOG" id="ENOG502SI3G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017660_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEM9"/>
    <dbReference type="OMA" id="PAKKMHN"/>
    <dbReference type="OrthoDB" id="10061051at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038740">
        <property type="entry name" value="BioF2-like_GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13480">
        <property type="entry name" value="Acetyltransf_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="194"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="BioF2-like acetyltransferase" evidence="3">
        <location>
            <begin position="627"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="738" mass="83863" checksum="A5C8BBDAE7822154" modified="2011-11-16" version="1">MKKERQASKCSGETDLSHKDAESHKESESSSLPEDSEKISPPPPIGKKLLWLFLFVITVLQVVVLTQGKFDNPFNIDADFKIFFNPSHSQRATFFAIKLVAIVFSGLLLCVWWSADISTIFLAFISANIIQVLIAIALIQINLDLINLTLSRPAKKMHNLFILTGCFLIGQSMIGVVISVLINDKNRLHELMGLLLFLYDTVVVLLGLLLLSCTILSGVKEGYHPMFIEEVPVSIPILFGCAVVMSLLCFACTRYFRKRTLTTNLLVEEYDLMGLTDYQKQGWAKLINLNKKYNAGISGEHIVSLMENYSHSSLEGMTCKILRVYRDKPHETPTKPGAINKFIKKKIEEKAKLNTAFNELDREAVLFQNDPNSKEEEEADPLEVYKPISKNALKKLQKKSKQKKQAMDLKSLEANTEKFYQELMETEALVLLTVIEEFDLCERIPGKVGTFLGKYFGKNGKYPILVVKFGLLGFHWPFKRSTFYCSATKKPVARGAAVLYTLSEWNKKNEKCTVLMDPLYSDYNFEEGVTYSGWYKIKLPNSHIIDLRPFRNQKPTDYFKAIKYRTQENTFKSANGEVWETDQFNYENCSSIINMNLNIANNRASSGQSSQLMYPDWEFISNLGNYSNENKYRTLLYLKVDGKIIASCVIFRLGETMTSDIQGLDHETSKKYKAYFVMMQEVIRMGLEEKVSFIDFGPTTEEAKVSIGCKVVPLTGCVYPRNAFMGPIIKFAASKVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AET5</accession>
    <name>G3AET5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_130748</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373177.1">
        <property type="nucleotide sequence ID" value="XM_007373115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AET5"/>
    <dbReference type="FunCoup" id="G3AET5">
        <property type="interactions" value="782"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AET5"/>
    <dbReference type="GeneID" id="18869527"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_130748"/>
    <dbReference type="eggNOG" id="KOG2273">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021752_0_0_1"/>
    <dbReference type="InParanoid" id="G3AET5"/>
    <dbReference type="OMA" id="EKMAAVW"/>
    <dbReference type="OrthoDB" id="271164at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030904">
        <property type="term" value="C:retromer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07627">
        <property type="entry name" value="BAR_Vps5p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06861">
        <property type="entry name" value="PX_Vps5p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000013">
        <property type="entry name" value="Putative Sorting nexin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000022">
        <property type="entry name" value="Sorting nexin 3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035803">
        <property type="entry name" value="BAR_Vps5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037868">
        <property type="entry name" value="PX_Vps5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015404">
        <property type="entry name" value="Vps5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF170">
        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09325">
        <property type="entry name" value="Vps5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="12">
        <location>
            <begin position="286"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="42"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="148"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="412"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="90"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="172"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="199"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="216"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="256"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="440"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW35765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="695" mass="79208" checksum="FB38C2175253AB78" modified="2011-11-16" version="1">MDNEDLTASHWDDVVSPNEFGSTSNTTATNSLHQSIITPLSNQFNGINIDDPFASPLGFSNQQQRHNDEEEEENDEEEEEELQQSRHQEFSSPSPFYDSNQAEYEQRNELRKEQRKEHKHQLLSELTHGSEDSELMLEAPIIPDDLTNSESLFQDKGSPIKVSKDAKPIIPSSPTKTINFKQGQFKARPRKFSAKLNVQHLSSSSNNQLGPLGGNEGEKSNDRKSPSVSNADSLVKQADAPLYSISRKTDNREPASASTESATVIESGSTIKKKPTVEDEDNPQNNKLDISVGDPMKVGDITTAHIVYTIKTTNKNLESDNFPKNDTTEVTRRYRDFRWIYHQLQNNHPGKIIPPPPTKQTYIGRFNENFIENRRLSLEKMLNKISHIPVLSNDPDFVMFLTSDDFINESRERERLSGSAASTQNSEFLDGARPSGDNESSTGSLTPISTGSTTTGGFMSSLFSISNKINEPDEYFSDQKQYIDDLEYNLKQFYKAIELIGQQRIDLIGILEEVSITVDELASLEISKITSDLLIGFSEIQLKIRDNLDRINLQDQLTLGFTIEEYLRIIGSIKFVFDTRLKIYQQYYNFNQELSKKQNQLDKLQRKYSRAAPQVDKINSLSFEVDKLKQKTSSYEASFNQISETIKLELENFEFERIDDFRNSVEIFIESSIESQKEAIELYETFYERQKLASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF14</accession>
    <name>G3AF14_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_132785</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome b5 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372230.1">
        <property type="nucleotide sequence ID" value="XM_007372168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF14"/>
    <dbReference type="FunCoup" id="G3AF14">
        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF14"/>
    <dbReference type="GeneID" id="18869692"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132785"/>
    <dbReference type="eggNOG" id="KOG0536">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046313_2_3_1"/>
    <dbReference type="InParanoid" id="G3AF14"/>
    <dbReference type="OMA" id="GHSQLDW"/>
    <dbReference type="OrthoDB" id="432299at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004128">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000001">
        <property type="entry name" value="Cytochrome b5 reductase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051872">
        <property type="entry name" value="Cytochrome_b5/Flavoprotein_Rdt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46237:SF1">
        <property type="entry name" value="CYTOCHROME B5 REDUCTASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46237">
        <property type="entry name" value="CYTOCHROME B5 REDUCTASE 4 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="6">
        <location>
            <begin position="82"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="159" mass="17571" checksum="5C79C2EA5483825F" modified="2011-11-16" version="1">MKSSNLKPPVSGPIRTPPSGGANRGLFPAPNSAQRASSSLSGPRHKVALKPGYSPLDWAQLNRTQPKYKLRGVDPTTPPAQFVKITKEELKKHKSEQDCWTCINGKVFNITPYINFHPGGVDEIMKCAGRDGTALFNKYHSWVNADRMLENCIVGIYVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFE2</accession>
    <name>G3AFE2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_145590</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372343.1">
        <property type="nucleotide sequence ID" value="XM_007372281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFE2"/>
    <dbReference type="FunCoup" id="G3AFE2">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFE2"/>
    <dbReference type="GeneID" id="18870692"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_145590"/>
    <dbReference type="eggNOG" id="ENOG502QQNH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015737_1_2_1"/>
    <dbReference type="InParanoid" id="G3AFE2"/>
    <dbReference type="OMA" id="WGDYKGT"/>
    <dbReference type="OrthoDB" id="5592486at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003386">
        <property type="entry name" value="LACT/PDAT_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11440">
        <property type="entry name" value="LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02450">
        <property type="entry name" value="LCAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="341" mass="38983" checksum="1B85AF34C0525FAE" modified="2011-11-16" version="1">MNRLQLRYMSVRRFAIGSRLKNTVKETQDQFLKQVPLSHYLAPKNPIVLCHGFSGFDKLTLIPGIRNLPTGAKKELENVATKGILEFDYWRGIQYALENIGAEVLIARVPAFSDIRSRAANLDHFLNRQCKIIQRKQSTNSPVTLNLISHSMGGLDSRYLISKLQNDKSHYKVSSLTTISTPHHGSECADFIVDLVGNRPVLSQLVPRSIFDMTTTNMATFNKIVVDDPEVAYFSYGARFNPRWYNFFGLTWLITKFKIQSKHSNESKYQKLVDNDGLVSVDSSKWGEYMGTFDEVDHLDLINWTNNARDTFTKIMFNQDPPFNPILLYLEIANNLAEKGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFE9</accession>
    <name>G3AFE9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Polyadenylate-binding protein, cytoplasmic and nuclear</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Polyadenylate tail-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_58066</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Binds the poly(A) tail of mRNA. Appears to be an important mediator of the multiple roles of the poly(A) tail in mRNA biogenesis, stability and translation. In the nucleus, involved in both mRNA cleavage and polyadenylation. Is also required for efficient mRNA export to the cytoplasm. Acts in concert with a poly(A)-specific nuclease (PAN) to affect poly(A) tail shortening, which may occur concomitantly with either nucleocytoplasmic mRNA transport or translational initiation. In the cytoplasm, stimulates translation initiation and regulates mRNA decay through translation termination-coupled poly(A) shortening, probably mediated by PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372350.1">
        <property type="nucleotide sequence ID" value="XM_007372288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFE9"/>
    <dbReference type="FunCoup" id="G3AFE9">
        <property type="interactions" value="1398"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFE9"/>
    <dbReference type="GeneID" id="18872304"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58066"/>
    <dbReference type="eggNOG" id="KOG0123">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_22_2_1"/>
    <dbReference type="InParanoid" id="G3AFE9"/>
    <dbReference type="OMA" id="MNGRMLN"/>
    <dbReference type="OrthoDB" id="19742at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140693">
        <property type="term" value="F:molecular condensate scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008428">
        <property type="term" value="F:ribonuclease inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060211">
        <property type="term" value="P:regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12379">
        <property type="entry name" value="RRM2_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12380">
        <property type="entry name" value="RRM3_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12381">
        <property type="entry name" value="RRM4_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1900.10:FF:000008">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000211">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000384">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.10">
        <property type="entry name" value="c-terminal domain of poly(a) binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036053">
        <property type="entry name" value="PABP-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045305">
        <property type="entry name" value="RRM2_I_PABPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63570">
        <property type="entry name" value="PABC (PABP) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="11 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM" evidence="17">
        <location>
            <begin position="15"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="17">
        <location>
            <begin position="108"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="17">
        <location>
            <begin position="211"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="18">
        <location>
            <begin position="411"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="356"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="362"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="384"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW34938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="508" mass="57140" checksum="E8769DD5CA3BA46C" modified="2011-11-16" version="1">MWSQRDPSSRRSGDGNIFIKNLHPAIDNKALHDTFSAFGKILSCKVATDDMGQSKCFGFVHYETAEAAEAAIENVNGMLLNDREVYVGKHISKKDRESKFEEMKANYTNIYVKNIDLEFSDEEFEKLFVPFGTITSIYLEKDQDGKSKGFGFVNYETHESAVKAVEELNDKEINGQKIYVGRAQKKRERMEELKKQYESTRVERLSKYQGVNLFVKNLDDSIDSEKLEEEFKPFGSITSAKVMVDEAGKSKGFGFVCFSSPEEATKAITEMNQRMILGKPLYVALAQRKDVRRSQLEQQIQARNQMRMQNAAASGGIPGQFIPPMFYGQQGFFPPNGRGNAPFPGPNPQMMMRRGQQFPGPEQWPRPGPNGQPVPVYGIPPQFQDFNGQPMRQQQRGGYYPNRNQNKGGRQQKDLAAIIANAPPDQQKRILGEELYPKIVATGKAQEPEAAGKITGMMLGLENQEILDLLEDDELFNNHFEDALTAFEEYKKSEAGAPAPAAPEQPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFF8</accession>
    <name>G3AFF8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">mitochondrial processing peptidase</fullName>
            <ecNumber evidence="5">3.4.24.64</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Beta-MPP</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_132720</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalytic subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins. Preferentially, cleaves after an arginine at position P2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.</text>
            <dbReference type="EC" id="3.4.24.64"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.64" evidence="5"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372359.1">
        <property type="nucleotide sequence ID" value="XM_007372297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFF8"/>
    <dbReference type="FunCoup" id="G3AFF8">
        <property type="interactions" value="911"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFF8"/>
    <dbReference type="MEROPS" id="M16.003"/>
    <dbReference type="GeneID" id="18869686"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132720"/>
    <dbReference type="eggNOG" id="KOG0960">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009902_4_2_1"/>
    <dbReference type="InParanoid" id="G3AFF8"/>
    <dbReference type="OMA" id="IDVVCDM"/>
    <dbReference type="OrthoDB" id="10251424at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017087">
        <property type="term" value="C:mitochondrial processing peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000002">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000001">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF149">
        <property type="entry name" value="GH01077P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 18" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="16">
        <location>
            <begin position="38"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="17">
        <location>
            <begin position="190"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW34947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="464" mass="50867" checksum="46FE7144EE9C5C31" modified="2011-11-16" version="1">MLRGSLKALTKQAGRRSFTTAVPGATYKTSVLPNGLTVASEFMPGTRTATVGVYINAGSRADSPTSSGTAHFLEHLAFKGTKKRTQLGLELEIENLGSQINAYTSRENTVYYTKCLARDVDQNLDILSDLLAQSRLENRAIENERHVILQESDEVDKMYDEVVFDHLHAVAYKSQDLGRTILGPREKIKTINRNDLVNYITTNYKGDRMALVGVGCVDHEELVEQAKKYFGKFKQSDVPFKQNGDDLPIFYGEEIRIQDDAMPTTHVALAVEGVSWSAPDFFVASVANGIIGTWDRTIGSGSNHPSPLAVTAATGGPGGTPIANSYMAYTTSYADTGLLGVYFTADKDADLKLFVDAVQKEWGRLSSGQITEEEVERAKAQLKAALVLALDDSTAIAEDIGRQVVNTGYRLSPEEVFERVESITKDDVVNWANYRWKNRPIALAAVGNVKTLPSHKEISKGMSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFN1</accession>
    <name>G3AFN1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_133224</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="8"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372433.1">
        <property type="nucleotide sequence ID" value="XM_007372371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFN1"/>
    <dbReference type="FunCoup" id="G3AFN1">
        <property type="interactions" value="951"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFN1"/>
    <dbReference type="GeneID" id="18869729"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133224"/>
    <dbReference type="eggNOG" id="KOG0373">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004962_0_5_1"/>
    <dbReference type="InParanoid" id="G3AFN1"/>
    <dbReference type="OMA" id="MCLKVKY"/>
    <dbReference type="OrthoDB" id="267048at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903432">
        <property type="term" value="P:regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000005">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="9">
        <location>
            <begin position="114"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="314" mass="35723" checksum="E3FC0B743C49731A" modified="2011-11-16" version="1">MGERGPDQWLEQIKKCIALTESDMKQLCELVKELLMEESNIQPVQSPVTVCGDIHGQFHDLLELFRISGGLPSDDNNTNYVFLGDYVDRGYFSLETFTLLMVLKVKYPHRITLVRGNHESRQITQVYGFYEECLTKYGSTTVWKYCCQVFDFLTLAAIIDGKILCVHGGLSPEIRMLDQIRVLSRAQEVPHEGGFCDLVWSDPDNVDTWAVSPRGAGWLFGSKVSREFNHINNLSLIARAHQLVMEGFRFHFKEKDVVTVWSAPNYCYRCGNVASVMQVDDYDEPNFKIFSAVQDGDLAVKNNGGKSSRSDYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFR3</accession>
    <name>G3AFR3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sphingolipid long chain base sensory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_132023</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372464.1">
        <property type="nucleotide sequence ID" value="XM_007372402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFR3"/>
    <dbReference type="STRING" id="619300.G3AFR3"/>
    <dbReference type="GeneID" id="18869631"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132023"/>
    <dbReference type="eggNOG" id="ENOG502QQ1T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046464_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFR3"/>
    <dbReference type="OMA" id="WGYDNDD"/>
    <dbReference type="OrthoDB" id="5599269at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036286">
        <property type="term" value="C:eisosome filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000005">
        <property type="entry name" value="Sphingolipid long chain base-responsive pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028245">
        <property type="entry name" value="PIL1/LSP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962:SF1">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13805">
        <property type="entry name" value="Pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="309" mass="34325" checksum="2EAF66ADB0730AC5" modified="2011-11-16" version="1">MHRTYSLRSAKAPTAAQLQSPPPPPSTTKNKFFGSGGLSSSIRKATAGATGPELSRKLAQLIKMEKNFMRAIEVTSRERKDVAKQLSAWGEGNDDDVSDVTDKLGVLIYEIGELEDQYIDKYDQYRITLKSIRDIEGSVQPSRERKQKITDDIAHLKYKDPQSPKIAVLEQELVRAEAESLVAEAQLSNITREQLKAAFNYQFDATRELAEKYALIAGYGKALLELLDDSSVTPGETRPAYDGYEASKQIIIDAENALASWTLDSAAVKPTLSFHQVEGEEDEEEIDEIDEAVEEEFAKHDEEVPANGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFS8</accession>
    <name>G3AFS8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_48116</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372479.1">
        <property type="nucleotide sequence ID" value="XM_007372417.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFS8"/>
    <dbReference type="GeneID" id="18871183"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48116"/>
    <dbReference type="HOGENOM" id="CLU_1750823_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFS8"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="149" mass="17575" checksum="4041EA0CF16A61BA" modified="2011-11-16" version="1">MEKPGISAADLSKFADAYCSDLEFLDDDLEHEEMNLDEIRNHILRKAKKRVIKINKTEDNKYKRFFIDGSTDIVSRKDVRKPALRNPEDVYAQIDKLEKHFASLDPITKLRFGVVETCERREIETPAERIFFGVYDKNESEDWWSDEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFU3</accession>
    <name>G3AFU3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein BTN</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_58222</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Vacuole membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the battenin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372494.1">
        <property type="nucleotide sequence ID" value="XM_007372432.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFU3"/>
    <dbReference type="FunCoup" id="G3AFU3">
        <property type="interactions" value="116"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFU3"/>
    <dbReference type="GeneID" id="18872369"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58222"/>
    <dbReference type="eggNOG" id="KOG3880">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029663_3_1_1"/>
    <dbReference type="InParanoid" id="G3AFU3"/>
    <dbReference type="OMA" id="ATILYCE"/>
    <dbReference type="OrthoDB" id="5965864at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903826">
        <property type="term" value="P:L-arginine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015819">
        <property type="term" value="P:lysine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003492">
        <property type="entry name" value="Battenin_disease_Cln3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981:SF0">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02487">
        <property type="entry name" value="CLN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01315">
        <property type="entry name" value="BATTENIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="343" mass="38495" checksum="BF22958F456EDD65" modified="2011-11-16" version="1" fragment="single">MHISEPQTIFISFFIFGLLNNVLYVIILSAAIDLVGSATPKAVVLLADIIPSFTFKLVSPFFIHLLSYPFRLWTILGLSMSGMLIISLTPNGLIGFKIFGIALASLSSGLGEVSFLQLTHYYEAKHSIGGFSSGTGGAGLFGSFLFMLFTNIMGIPVWLTLFMSSVVPAGLLLTYNFLLPPPELEYEPISENIVEEGELAFTENKFDLHYMKNHISETITEIKPLIIPYMLPLTTVYASEYTINQGVSPTLLFPLDDLPHWLFSTYRDLYVVYGFLYQLGVFISRSSISFGIRIKHLYVMSMLQFVNLVITVCQSMYDFPFHRIWLLLVLILYEGLLGGFSYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGM8</accession>
    <name>G3AGM8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58590</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372779.1">
        <property type="nucleotide sequence ID" value="XM_007372717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGM8"/>
    <dbReference type="FunCoup" id="G3AGM8">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGM8"/>
    <dbReference type="GeneID" id="18872519"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58590"/>
    <dbReference type="eggNOG" id="ENOG502QRM9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017395_2_1_1"/>
    <dbReference type="InParanoid" id="G3AGM8"/>
    <dbReference type="OMA" id="TNKACHK"/>
    <dbReference type="OrthoDB" id="2414723at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31758">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING PROTEIN YLR108C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31758:SF2">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN YLR108C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="316" mass="36113" checksum="561CE6E6D8E0BD90" modified="2011-11-16" version="1" fragment="single">ILTEQEIFASVGGVSFKIPKVLFGHSEGNYPNYFTMNYDRMLMDNILIVEQQKMLRPPPQRPANVANRSPKLFSDLLELLRGNEEVIASDNHRKLLIRECRYYRFLELEQRILKHNIITYQGEPAIVINLLDLTRKGILNISPPDKKTETPLLYTRPYIPREPSRCLIVQIDEPENSQIKLILNKSTGLPTVKITNKLCTKITQTFKDYLESAITTGLNSDTPAITFFAGFKDSKCTVNGQPVKDNWIEELLGVSEDNPTKKRKKSDDGEAKGDIIAFRLKRSMWKLMMRGSFSRLHAVSVEAFTDVSNSETIDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGN9</accession>
    <name>G3AGN9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_63860</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372028.1">
        <property type="nucleotide sequence ID" value="XM_007371966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGN9"/>
    <dbReference type="GeneID" id="18874948"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63860"/>
    <dbReference type="eggNOG" id="ENOG502RB2N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_552275_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGN9"/>
    <dbReference type="OrthoDB" id="4056983at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="494" mass="58146" checksum="6DE1EE82572F77BE" modified="2011-11-16" version="1">MISATIPKNFITFLKKKWGYDCGRYINLITSDPLAKIFNTSTSFKERDRIMDYVVVSMKNFLQRDVNDIALLYFENKIIQENYYRRLCSEFGDKLMVMINADTENKQASLLRLTRGARVLLGTKSVRREPAEDSETSKDYECMDNSSDYDPCEERIRVSKCLTKQVAHFYGIKVSKCQNCCDSNDSILEPHIVDLVNNVHCATETQFKELQFGTEQAPAPKKFRSWTAEERLDHFALQFEPEEWLEELIDNGAKYLKYPLTHMDALPESEYICTKCCRKHVKECKRGGFKALLVLKWILDDITYDDIKDMKVNGWGDEIYQWAQNHNLKHLYDTYLDKVEQFTNCLRVPSLFSVDDYGNQLDHLRKFLRHPFFLNEYLLEDPIPTEDAGIYEKAQRYGEEYMHADTRKLIQMSQFHNNYCRQCGNSKHSGECRYSQAIELFYIIFCIPAFRDLRRSRQLGLTINYFPHWLDAISKNGHHICILNDILGRDRVWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGS0</accession>
    <name>G3AGS0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Lipoyl synthase, mitochondrial</fullName>
            <ecNumber evidence="10">2.8.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Lipoate synthase</fullName>
            <shortName evidence="10">LS</shortName>
            <shortName evidence="10">Lip-syn</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Lipoic acid synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_133253</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>[[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine + 4 H(+) = [[Fe-S] cluster scaffold protein] + N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 5'-deoxyadenosine + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:16585"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:14568"/>
            <dbReference type="Rhea" id="RHEA-COMP:14569"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:33722"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="2.8.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10">Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the radical SAM superfamily. Lipoyl synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.8" evidence="10"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372815.1">
        <property type="nucleotide sequence ID" value="XM_007372753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGS0"/>
    <dbReference type="FunCoup" id="G3AGS0">
        <property type="interactions" value="596"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGS0"/>
    <dbReference type="GeneID" id="18869731"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133253"/>
    <dbReference type="eggNOG" id="KOG2672">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033144_2_0_1"/>
    <dbReference type="InParanoid" id="G3AGS0"/>
    <dbReference type="OMA" id="PYCDIDF"/>
    <dbReference type="OrthoDB" id="3231at2759"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00593"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016992">
        <property type="term" value="F:lipoate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000036">
        <property type="entry name" value="Lipoyl synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00206">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031691">
        <property type="entry name" value="LIAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003698">
        <property type="entry name" value="Lipoyl_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00510">
        <property type="entry name" value="lipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004019">
        <property type="entry name" value="PRK05481.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009544">
        <property type="entry name" value="PRK12928.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949">
        <property type="entry name" value="LIPOYL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949:SF0">
        <property type="entry name" value="LIPOYL SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16881">
        <property type="entry name" value="LIAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00271">
        <property type="entry name" value="lipoyl_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01058">
        <property type="entry name" value="lipoyl_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="7 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="11">
        <location>
            <begin position="123"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW35403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="390" mass="43452" checksum="02D926AB7AC940B4" modified="2011-11-16" version="1">MLSIQTNLLKKQSCIFVRSLATATAATPGPTPVSRRRPTKFSDKLNKGPSFDDFVSGKAQDMLEDPLEAARKDPNQRLPSWLKVPIPKGKSFHNVKNDVRELKLATVCEEAKCPNIGECWGGKKSEATATIMLMGDTCTRGCRFCSVKTNRAPAPPDPKEPENTAEAISRWGLGYVVLTTVDRDDLIDGGANHLAETVRKIKQKAPQILVEVLSGDFRGDLDMVNILATSGLDVYAHNLETVEDLTPHVRDRRATYRQSLAVLERAKQAKPTLVTKTSLMLGFGETDDQVMRTLKDLRSVGCDVVTFGQYMRPTKRHMKVVEYVTPEKFDYWRDVALEMGFLYVASGPLVRSSYKAGEAFIENVIKKRRHNVGEAPRLEQEIKPALFSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH90</accession>
    <name>G3AH90_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RRM Nup35-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_58752</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372932.1">
        <property type="nucleotide sequence ID" value="XM_007372870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH90"/>
    <dbReference type="FunCoup" id="G3AH90">
        <property type="interactions" value="181"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AH90"/>
    <dbReference type="GeneID" id="18872590"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58752"/>
    <dbReference type="eggNOG" id="ENOG502QWFW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036683_0_0_1"/>
    <dbReference type="InParanoid" id="G3AH90"/>
    <dbReference type="OMA" id="TIHEHTP"/>
    <dbReference type="OrthoDB" id="1733656at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044615">
        <property type="term" value="C:nuclear pore nuclear basket"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12721">
        <property type="entry name" value="RRM_Nup53p_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007846">
        <property type="entry name" value="RRM_NUP35_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21527">
        <property type="entry name" value="NUCLEOPORIN NUP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21527:SF6">
        <property type="entry name" value="NUCLEOPORIN NUP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05172">
        <property type="entry name" value="RRM_Nup35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51472">
        <property type="entry name" value="RRM_NUP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6 8" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM Nup35-type" evidence="10">
        <location>
            <begin position="188"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="46"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW35520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="373" mass="41807" checksum="29CD6E4E93C063BA" modified="2011-11-16" version="1">MSLFTQSTSLNPTINPSVQQQPVQQPQQQQQVSTPVANNSWYTQNQKKRTIPNHLIPKKKPGFQITASTTSKKKSSKSPTTSSGLLLGDSHQFNMLSFGNQQRKTISSGIFDDDDTTIDNSEEGNSLLVYQDDLPPNKSLFELNEKVNKPIDQIDSFINKDPRKFSNLFNKNEEVEPVPVVKPENQLSHSESAILVFGYPESLSNQVIQLFKQFGDILEDFQINKTKSLYTTQANNKVVPIFSGPNWVKITYDNPTSAIEALQENGTVFNGVLLGVIPYHKSALENLQKRSLIDKENIGEMNLNIQLPAQESESKSSDVAPPTTDPYNARLDIKEGKQFFVAPEGTELKKPNNSERLSWIGTISKYVFGFHDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHC2</accession>
    <name>G3AHC2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59508</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373670.1">
        <property type="nucleotide sequence ID" value="XM_007373608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHC2"/>
    <dbReference type="GeneID" id="18872948"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59508"/>
    <dbReference type="eggNOG" id="ENOG502RQAU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1120504_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHC2"/>
    <dbReference type="OMA" id="WIMNAIS"/>
    <dbReference type="OrthoDB" id="4025436at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="249" mass="29770" checksum="3E8AD9A8A7DD67D2" modified="2011-11-16" version="1">MLHSVINSQPSSPSPQNGAIHHKKVLPRDEDVKLSHIKQERMRSPVPDFAMSHFLNDEISYLYWFQDIEENLQLYPQFNPYFVLDKIPLSGYTLDYQDKVKKATAKCIENPDVNQYLNFQLYNSINQSLHYLVGDKTFIENFEAIKFHFHQTINPFYLMTLEHQLSFDLNNILKFLTDLDNLTKVYTLTFQRPPTNELKLEWIMRCLNHYSDISREIQLNWEQINKDPILIRRILTKYANSRMKLRKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHE6</accession>
    <name>G3AHE6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_59532</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373694.1">
        <property type="nucleotide sequence ID" value="XM_007373632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHE6"/>
    <dbReference type="GeneID" id="18872960"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59532"/>
    <dbReference type="eggNOG" id="ENOG502T2NA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1826505_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHE6"/>
    <dbReference type="OrthoDB" id="3993572at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="141" mass="16608" checksum="152DFA64FF3477FC" modified="2011-11-16" version="1">MFPKFLLKLLKQTNKIWVLILVFLIRKTISQLLNVVRKIQKVEIEYSLLNKQAEKTSSMIHEDICKKYNKVLKDLKFDKVMLIIELIGNFLDLSFNLIELYGIVLPDWVMGGLNFASMAMTIYRMNKDDEYLDDDITEDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI24</accession>
    <name>G3AI24_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59750</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373922.1">
        <property type="nucleotide sequence ID" value="XM_007373860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI24"/>
    <dbReference type="GeneID" id="18873063"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59750"/>
    <dbReference type="eggNOG" id="ENOG502RPYY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1220350_0_0_1"/>
    <dbReference type="InParanoid" id="G3AI24"/>
    <dbReference type="OrthoDB" id="4026747at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="227" mass="25165" checksum="338276620D098EEB" modified="2011-11-16" version="1">MARTNSVPPPPEKPQLLRSRTTGIFHIENQMENNKPSIVFTNKFPSSTDLTTSEEGTPVPMVKQETIVAVSDVNAISTTDHKEQDHARLSTSLNKISRSLSHHSFRNLLSRSQLNLTKMYQKPKPKPESIKQPTPQVQQPVQLSPSYSPPVPIVPRRPVTTTTIELKPNDKLLDESLSASLKKALSIDYKLGKVPLPERVIDDRAIVAGPGGGSLDSDFDDYHSKGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI85</accession>
    <name>G3AI85_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_48783</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373238.1">
        <property type="nucleotide sequence ID" value="XM_007373176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI85"/>
    <dbReference type="GeneID" id="18871268"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48783"/>
    <dbReference type="HOGENOM" id="CLU_1180850_0_0_1"/>
    <dbReference type="InParanoid" id="G3AI85"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442347" evidence="2">
        <location>
            <begin position="21"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="175"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="235" mass="24072" checksum="C44CC4EED10573FA" modified="2011-11-16" version="1">MCFFINTMLLLLVLDSVASSDPQDRPAASSEGSSGSSNTPDKQSSTRVASNIITGVSSNALPTSPPQDTTPSSVTIVVTTVTKTDITLVSTVTYSNGYPQSTSNTQPGNNQQVSNSSADSKGPSDPDYLAVDSNSTPESGNSNGASNSSGYSNNDNSGSQDFKTPSAGPDSVVPNNINSGNEQNQGSSSVGGGATNPDDANSTLPELQEGYKDSATGLRANTFVACLFFICIIFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AID4</accession>
    <name>G3AID4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Carbonic anhydrase</fullName>
            <ecNumber evidence="5">4.2.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Carbonate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_59837</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="5"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373988.1">
        <property type="nucleotide sequence ID" value="XM_007373926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AID4"/>
    <dbReference type="STRING" id="619300.G3AID4"/>
    <dbReference type="GeneID" id="18873097"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59837"/>
    <dbReference type="eggNOG" id="ENOG502RXIV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084253_1_1_1"/>
    <dbReference type="InParanoid" id="G3AID4"/>
    <dbReference type="OMA" id="ECGMSGL"/>
    <dbReference type="OrthoDB" id="10248475at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03379">
        <property type="entry name" value="beta_CA_cladeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43175:SF3">
        <property type="entry name" value="CARBON DISULFIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43175">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="beta-carbonic anhydrase, cab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601765-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="161" mass="17689" checksum="D1AB28DF23886B17" modified="2011-11-16" version="1">MTVASEFEQANKSYVDQFTKGSLPLPPARKVAVVVCMDARIDPAAALGLTEGDAHVIRNAGGRATDALRSVIISQRLLGTREIVVIHHTDCGMLTFTDEGLRKIVEDETGQNVDHFAFLPFNDLEKSVKDDVEFFKKNPLVLDVPVTGYIYDVKTGKITQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ01</accession>
    <name>G3AJ01_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_149506</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374097.1">
        <property type="nucleotide sequence ID" value="XM_007374035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ01"/>
    <dbReference type="FunCoup" id="G3AJ01">
        <property type="interactions" value="1205"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ01"/>
    <dbReference type="GeneID" id="18870828"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_149506"/>
    <dbReference type="eggNOG" id="KOG0152">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005825_1_2_1"/>
    <dbReference type="InParanoid" id="G3AJ01"/>
    <dbReference type="OMA" id="NEPIYKH"/>
    <dbReference type="OrthoDB" id="187617at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.440">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002713">
        <property type="entry name" value="FF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036517">
        <property type="entry name" value="FF_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039726">
        <property type="entry name" value="Prp40-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11864">
        <property type="entry name" value="PRE-MRNA-PROCESSING PROTEIN PRP40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11864:SF0">
        <property type="entry name" value="PRP40 PRE-MRNA PROCESSING FACTOR 40 HOMOLOG A (YEAST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01846">
        <property type="entry name" value="FF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00441">
        <property type="entry name" value="FF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81698">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51676">
        <property type="entry name" value="FF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="FF" evidence="3">
        <location>
            <begin position="330"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="414"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="598" mass="70094" checksum="A3CE6062FBE20627" modified="2011-11-16" version="1">MSDWEKVTSEDGQVYYYNSKTNETSWTLPEETAVVTGSQWEEYTTEDGRTYYYNESTGETTWEKPAKLETKKEETVAKEEESEADLELANKPVVLPAIEKKEEEGEETEDAEKAFVKLLSDNNVDSTWAFQAVMEKFITFPEYWNVPNPLKRKQLYEEYLVSKFQDELSNKTLLIENFKTNFIEELKKLQEKESMDYNTRWITVRKVLIDQDNPIFKHAILSDSELAQLYYEYTDDLKAERNSFIEQQKEQALSELEAYLTQINPSLVTESKNWQELYDKILVDPRFKANKHFDILNKVDILTLYEQKIYPTIIENIRTQIKTIEKQNYRNDRKARDNFKTLLQTLKIEAVTTFKDVLPILENEDSFIEICGRNGSSPLELFWDIVDEKKQLLKVKKDLVEGVLLDLKNQDPEANSMENLLKSQEELISKLSQVKDDRLSSFDFKETHELEMIYISLRNEFEIQKEQEKKRFEKALQLAEHDFGEWLADNYEQLDIIGEEESKLIPIQIETTSIGKIYTLTASIDYQAVSDQLTQLDGYKSISSLATDKSIDLVDIAKRSLQEFVRIMTKRSSKKRTLSSSDREFKKFKADSSVLLNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ11</accession>
    <name>G3AJ11_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_65651</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetate uptake transporter (AceTr) (TC 2.A.96) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374107.1">
        <property type="nucleotide sequence ID" value="XM_007374045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ11"/>
    <dbReference type="GeneID" id="18875055"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65651"/>
    <dbReference type="eggNOG" id="ENOG502QUJS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051062_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ11"/>
    <dbReference type="OrthoDB" id="3648309at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015123">
        <property type="term" value="F:acetate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051633">
        <property type="entry name" value="AceTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000791">
        <property type="entry name" value="Gpr1/Fun34/SatP-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038013">
        <property type="entry name" value="AceTr_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123:SF1">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01184">
        <property type="entry name" value="Gpr1_Fun34_YaaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="263" mass="27813" checksum="21B521DD0B67334B" modified="2011-11-16" version="1">MSKDQEVYSIRDLSSSFEESSSSSISKTVTRVVTDNEFVILGNQKFYTHELMSVFGPTLNLGITPVSANKIGNPAPVGMLTFGLTCFIMSLYGVHAGGITISNAIVGMVIFVGGTGQVICGIGEMIIGNTFGATAMLSFAMFWFSYAAILIPSFGIEAAYAEHMDQFENAIGFLLMGYAILAIGLTTLTLKAAVSIAVLFVCISMTFLLQSIGAMVGNANVTYAGQVFGIFTGIAGMYNGLAEVSLKENSYFSVPVGPLSSQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ15</accession>
    <name>G3AJ15_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_49563</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374111.1">
        <property type="nucleotide sequence ID" value="XM_007374049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ15"/>
    <dbReference type="GeneID" id="18871350"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49563"/>
    <dbReference type="eggNOG" id="ENOG502RQFG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_502467_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ15"/>
    <dbReference type="OMA" id="ATIDISM"/>
    <dbReference type="OrthoDB" id="7451790at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050836">
        <property type="entry name" value="SDS22/Internalin_LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652">
        <property type="entry name" value="LEUCINE-RICH REPEAT AND IQ DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652:SF3">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="539" mass="63772" checksum="E2CCDBEB2B05E219" modified="2011-11-16" version="1">MFNIELLKECPDDIIFKILDHNFLAVHDIYNFLFYKSTHDVAQQVLNKRSLIHLTIGKRKNCESVITSSHDYEITKGPYFWHIYYNYANKDLFLSWYDRHKYIQNYVVQIFLDQFQFESLQFLQILKYKKIKIYLNYESDFNHTVRKFTHIIWPMIGEIFDLSNNFVNLILEYESSIDQNLTIDLSNLNQFEFRHYTPTYRSIEFKVNDKLQELKINNISMLPITIKLSSIPLNITQFLCNGPIANLVYLGHFLTKCPNLQKLSISKAHLSNFPDFIDIISPMGLPRLAWLDLSNNEFGNIEDLDLSTIFPNLSTFIMKFEQLKTHRFRFSDITFPDTLTSLILHDKGISKFTNIEGIKFLKYLDLSYNYPQDFEIPQRVSHITTLNLSYNRTILSSIYRFNRRDISNYIFFHVTELHLQGCNITNEDLEHLEADYQHSKHLPKSCVEYLDLSNNKLSNLRSFSGKLFTNLPLKYLDLSFNAFTYLNKDIFPITRQIYPNLSKVNLTGNARLHNITLSNDYPELELMYTPFERTKPTNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ35</accession>
    <name>G3AJ35_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">SPAPADRAFT_134536</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP = N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13501"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="Rhea" id="RHEA-COMP:10506"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:83145"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hydrogencarbonate + acetyl-CoA + ATP = malonyl-CoA + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11308"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374131.1">
        <property type="nucleotide sequence ID" value="XM_007374069.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AJ35">
        <property type="interactions" value="836"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ35"/>
    <dbReference type="GeneID" id="18869829"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134536"/>
    <dbReference type="eggNOG" id="KOG0368">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000395_5_2_1"/>
    <dbReference type="InParanoid" id="G3AJ35"/>
    <dbReference type="OMA" id="PTPKGHC"/>
    <dbReference type="OrthoDB" id="14612at2759"/>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004075">
        <property type="term" value="F:biotin carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001295">
        <property type="term" value="P:malonyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.460.10:FF:000001">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000005">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000010">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.460.10">
        <property type="entry name" value="Biotin dependent carboxylase carboxyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1770.10">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049076">
        <property type="entry name" value="ACCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049074">
        <property type="entry name" value="ACCA_BT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013537">
        <property type="entry name" value="AcCoA_COase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728:SF3">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08326">
        <property type="entry name" value="ACC_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21385">
        <property type="entry name" value="ACCA_BT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0092">Biotin</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="18">
        <location>
            <begin position="47"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="17">
        <location>
            <begin position="205"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="16">
        <location>
            <begin position="683"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="19">
        <location>
            <begin position="1477"/>
            <end position="1811"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="20">
        <location>
            <begin position="1815"/>
            <end position="2130"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="2160"/>
            <end position="2187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EGW34547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="2221" mass="248468" checksum="B20690DF739A159E" modified="2011-11-16" version="1">MSDTPTTKDYSSLHPSLPDHFLGLNSVATAEPSKVTDFVKAHQGHTVISKILIANNGIAAVKEIRSVRKWAYETFGDEKAIQFTVMATPEDLEANAEYIRMADQMVEVPGGTNNNNYANVDLIVEIAESTNVHAVWAGWGHASENPLLPEKLAASPKKIVFIGPPGSAMRSLGDKISSTIVAQHAQVPCIPWSGTGVNEVEIDPTTNLVSVSDHIYQQGCCTGPEDGLKKAKEIGFPVMIKASEGGGGKGIRKVDSEDNFVTLYNQAANEIPGSPIFIMKLAGDARHLEVQLLADQYGTNISLFGRDCSVQRRHQKIIEEAPVTVAKKDTFHQMENAAVRLGKLVGYVSAGTVEYLYSHAEDKFYFLELNPRLQVEHPTTEMVTGVNLPAAQLQIAMGIPMHRIRDIRTLYGVDPHTTTDIDFEFKTESSLTTQRRPAPKGHCTACRITSEDPGEGFKPSGGSLHELNFRSSSNVWGYFSVGNQSSIHSFSDSQFGHIFAFGENRQASRKHMVVALKELSIRGDFRTTVEYLIKLLETPDFEDNTITTGWLDELITKKLTAERPDPILAVVCGAVTKAHIQAEEEKKEFIQSLERGQVPHKNQFKTIFPVEFIYEGERYKFTATKSSADKYTLFLNGSRCFVGARTLSDGGLLCTIGGKSHSVYWKEEASATRLSVDGKTCLLEVENDPTQLRTPSPGKLVKYLVESGDHVNAGQPYAEVEVMKMCMPLIAQENGTVELIKQPGSTVNAGDILAILALDDPSKVKHAKPFEGTLPEMGEPNVTGTKPAHRFTYYASILQNILGGYDNQVIMKSTLESLTQVLKDKELPYSEWQQIISALHSRLPAKLNDSLTSLVERNQSRNAEFPARQILKQISKSVSESDDSMLKDVVEPLVVIATRYQNGLEEHEYDFFAGLIDQYYQVESLFSGENVREDDVVLKLRDENKSDLQKVIGICLSHSRVSGKNNLIIAILQAYQPLLQGNSSVATAIRESLKKLVQLDSRVCSKVALKAREISIQCSLPSIQERSDQLEHILRSAVLQTSYGEIYAKHREPNLEIIKEVVDSKHTVFDVLAQFLVNPDEWVAIAASEVYVRRSYRAYDLTDINYHFHDRFPIIEWKFRLPDMASSRYNTIQQPSSDDDHATTMKHAASVSDLSFVVDAKTEQLPRTGVLVPARHLDDVDEMLIAALETYQGGDGISFQANGDKADIPLLNVLNIVITNIDGYENEEEILGRVHDILDEYKQDLKNAGIRRITFVFAHKIGTYPKYYTFTGPDYVENKVIRHIEPALAFQLELSRLANFDIKPIFTDNRNIHVYEAVGKNAPADKRFFTRGIVRPGSIRDEITISEYLIAESNRLMADILDALEVIDTSNSDLNHIFINFSNVFNVQPHEVEAAFGSFLERFGRRLWRLRVTGAEIRIVCADQQGNSFPLRAIINNVSGYVVKSELYMEVKNAKGDWVFKSIGNPGSMHLRPISTPYPAKESLQPKRYKAHNMGTTYVYDFPELFRQATISQWKKYGKKAPKDVFTSLELIHDENDHLTAVEREPGSNKIGMVGFKVTAKTPEYPRGRQFIIVANDITHKIGSFGPEEDYFFNKCTELARELGIPRIYLSANSGARLGVASELIPLFDVAWNEEGAPDKGFKYLYLTPETKRAIENDGKGDSIITERIVENGQERYVLKTIVGSEEGLGVECLKGSGLIAGATSRAYKDIFTITLVTCRSVGIGAYLVRLGQRAIQIEGQPIILTGAPAINKLLGREVYTSNLQLGGTQIMYKNGVSHLTANDDLEGVQKIMEWLSYVPAQRGMPVPILESEDPWDREIDYYPPKQEAYDVRWMIEGREVDGEFESGLFDKGSFQETLSGWARGVVVGRARLGGIPIGIIGVETRAVENLTPADPANPDSTESMVQEAGQVWYPNSAFKTAQAINDFNNGEQLPLMILANWRGFSGGQRDMYNEVLKYGSFIVDALVDFKQPIFTYIPPNGELRGGSWVVVDPSINPDMMEMYADVEARAGVLEPEGMVGLKYRRDKLLATMQRLDPTYAQLKARLNESNLSPEEHAQINAKIVAREKALLPIYAQISVQFADLHDRSGRMLAKGVIRQEIKWSDARRYFFWRLRRRLNEEYVLKLIGEQVKNANKLEKVARLKSWMPTVDYEDDQAVSAWIEEHHAKLQKRIEELKQETSRSNIAKLLQEDPSTAVSAMKDFIETLSEEQKESFLKSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJA8</accession>
    <name>G3AJA8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">GTPase-activating protein of the rho/rac family</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_54685</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374151.1">
        <property type="nucleotide sequence ID" value="XM_007374089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJA8"/>
    <dbReference type="FunCoup" id="G3AJA8">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJA8"/>
    <dbReference type="GeneID" id="18871880"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54685"/>
    <dbReference type="eggNOG" id="KOG1703">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2710">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001321_1_0_1"/>
    <dbReference type="InParanoid" id="G3AJA8"/>
    <dbReference type="OMA" id="QCSAKFF"/>
    <dbReference type="OrthoDB" id="20689at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000755">
        <property type="term" value="P:cytogamy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035024">
        <property type="term" value="P:negative regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090334">
        <property type="term" value="P:regulation of cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09391">
        <property type="entry name" value="LIM1_Lrg1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09392">
        <property type="entry name" value="LIM2_Lrg1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN LRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215:SF10">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN LRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="8">
        <location>
            <begin position="112"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="8">
        <location>
            <begin position="179"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="8">
        <location>
            <begin position="534"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="9">
        <location>
            <begin position="923"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1211" mass="137167" checksum="E7A50B390F44853B" modified="2011-11-16" version="1">MSHTPNWTAMPDNTYSPEDTYRQQKQDRNEFNLVSRPSSSAPPNTTYGSSLSTPAPQRPYQERTNKSTSSTQLSPSHRHSIAVSPTPDKQPSLTDTSSLDSDAKKVRKRNKKTCNKCGLEITGQFVRALGCAFHVECFTCNECGKQCSAKFFPYEIKDTNGNVKQVALCEYDYFKKLDLICFNCNCALRGPYITALGNKYHLEHFKCTVCKRVFESDESYYEHESNIYCHYHYSKLYASHCEGCHSSIVKQFVELFRGGRKQQWHPECYMVHKFWNVCITSDSVGLQQLYGLSEESLQNIKALTTTSDKDEEDAVNSILLVHIEQQIEQVVMRCWLTLSGYEETTATCISDMLVNACTGDKFNGLIVTGKLILSVEVLFNALDAIFEMCGSSNDLLQEKFQRLPEQHTYDDSESSLTSPNEEYFQPLKKEPRNITGKIMSYLAILRKSDHIAKSGRLSAELLSVITGCAHYLKLLIGIGLNNALRLNKLYGTTTAIDKFLTLTSEYESFTFDEANEKNLHMLNAKLSIPINSTDACRLCGKSIEKSCFRNGSHRWHIKCFECSVCHNPISIEEADLCKVDLTDRLVCNNCNKDGGEVGGFEVISDLAQLSYLLKIALFRSRTVMKIDFMQIPVLETALNRRLSIREIPEESPVDSYSQTLSDVTRLRTKRQSQKLSRSVKKNARKSVIVEAPEADKASKFEIRNDDHIAEELADLSFDSPQIIQPKRMDSGSSSLSFDTQKSEKVVVKKSLKIRDEPQRQLTNTQLDRTSDMLKNEKSLTLDDIPRIVAAEQARDQRPNAFKHHNSLYQRQQVKMKGSGGSRSPSNMMSPSGALDNILSANHSQAPSSIDHPSVKQNKYYSELTKSEHFMLRHIAVEALSRISSNYNRDDLLPLIQIKKSSTFWDKFKFSSGDKEKTTNVFGTDLLELTKKYGVDSDLGVGPNKLRVPIVIDDVINALRQKDMSVEGIFRLNGNIKKLRELTDEINKNPSKSPDFSIQTAVQLAALMKKWLRELPNPLLTYNLYDVWISSQREPNPVMRKRILQLIYCMMPRSHRNLVEVLLYFFSWVASFSEIDEETGSKMDTHNLATVIAPNILISKSASQNNDNIQTGGDNYFLAIEVVNQLIELHEELSIIPHDLLEFYESCGLGKQEKELSTKEIMNVIDKYIKENPSCFDVDVRDGISNVEVKSNTVSKSQNRVGGELNGETQEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJN1</accession>
    <name>G3AJN1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">tRNA methyltransferase, has a role in tRNA modification</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_59316</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373516.1">
        <property type="nucleotide sequence ID" value="XM_007373454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJN1"/>
    <dbReference type="FunCoup" id="G3AJN1">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJN1"/>
    <dbReference type="GeneID" id="18872863"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59316"/>
    <dbReference type="eggNOG" id="KOG1331">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029501_2_0_1"/>
    <dbReference type="InParanoid" id="G3AJN1"/>
    <dbReference type="OMA" id="VHEVYQQ"/>
    <dbReference type="OrthoDB" id="271595at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106335">
        <property type="term" value="F:tRNA (5-carboxymethyluridine(34)-5-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006448">
        <property type="term" value="P:regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051422">
        <property type="entry name" value="AlkB_tRNA_MeTrf/Diox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069:SF21">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="58"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="184"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW33932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="280" mass="32122" checksum="DDB109A579D1CF41" modified="2011-11-16" version="1">MSVPSVSIERGLDPKAQEQDFVHDVYNEIASHFSQTRYKPWPIVEKFLKTRSDYSIGLDVGCGNGKYLGINDKLFIIGTDHSEGLVRCGQDISENSYNLGIADGLSLPHPESRFDFAISIAVIHHFANEERRVQAISHILSKLKVGGECLIYCWALEQEKSRRGYKEGDDQDILIPWVLKKPQPKKEKKKKSKMMPQDSTVESTPELTEKSESEDPLEQHSANTPKKEEPKEEEQDQTKYRYYHLYRKGELSENALKTGSCTIVDEGYEKDNWWVIIKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJX7</accession>
    <name>G3AJX7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_59440</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373612.1">
        <property type="nucleotide sequence ID" value="XM_007373550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJX7"/>
    <dbReference type="FunCoup" id="G3AJX7">
        <property type="interactions" value="282"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJX7"/>
    <dbReference type="GeneID" id="18872918"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59440"/>
    <dbReference type="eggNOG" id="KOG2722">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026460_2_0_1"/>
    <dbReference type="InParanoid" id="G3AJX7"/>
    <dbReference type="OMA" id="WSWGYHI"/>
    <dbReference type="OrthoDB" id="2499604at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31794">
        <property type="entry name" value="AUXIN EFFLUX TRANSPORTER FAMILY PROTEIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31794:SF2">
        <property type="entry name" value="AUXIN EFFLUX TRANSPORTER FAMILY PROTEIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="352"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="382"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="419"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="445" mass="49127" checksum="49ADD5D101B36A00" modified="2011-11-16" version="1">MLNLILSVQSAVPDLSFIDITLLTFEAVLEVVIICFAGFIAAHSGILTNSGQKVLSALNVDLFTPCLVLSKLASSLSIKKIADIIIIPIFYAVSTGIAYLCSRFTSYLLSLNEPETDFVTAMAVFGNSNSLPVSLTLTLAYTLPDLLWDDIEGDTNDGVASRGILYLLIFQQLGQVLRWSWGFNTLLRRRSRVELNTYYTKNGVIIHHDETTTLLNDEQTLYMDGNTSQDSSIEPQQGQESVVTIDPTKGDEFLPWYKNIKNLPFIKQFLAFMNPPLYAMLVSVVIASVPALQNAFFGNNDSFIHNTVTKSVTALGSVSIPLILIVLGSNLYPSSDIPPASKHYKRILIGSLLSRMILPSLFLLPIIALCVKFINVSILDDPIFLIVAFILTISPPAIQLSQISTLNEVYQKEMAGVLFWGYVVLTLPSTIFIVVCSLEVLKWAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJY2</accession>
    <name>G3AJY2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_59453</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373617.1">
        <property type="nucleotide sequence ID" value="XM_007373555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJY2"/>
    <dbReference type="FunCoup" id="G3AJY2">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJY2"/>
    <dbReference type="GeneID" id="18872921"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59453"/>
    <dbReference type="eggNOG" id="ENOG502RYK1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052853_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJY2"/>
    <dbReference type="OMA" id="TNKSWEQ"/>
    <dbReference type="OrthoDB" id="244495at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF27">
        <property type="entry name" value="PHO85 CYCLIN CLG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="2">
        <location>
            <begin position="133"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="310"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="372" mass="42188" checksum="2C8F314FF88404D3" modified="2011-11-16" version="1">MYQVHYPHHQYPLHGLGTAGHRTSSYLPPQQQPPQPPPQQSAHYNSYYQPQPTHVPSHSLYSYQQVAAPPQQVYNQSVYTPVINHEEEQQPAVNGGINSVLEYNLNQMASFLSWCGFGMLKQSRNPSLEFEQLIVSVLFATRLPKSTIIIALEYMNQRFSSDASITSKVLTEQEIFNHLVVSLVLANKFNDDNTFTNKSWCGATGLDLLKLNKMEKEWLEYVKWSLNVVNFETNILTLEECWKTWLEKYSHAPSASNSPISISSPCSSSPRSQLNSSPITRPHSSYSSIPSSPEYDQKYYALKSSWGQHQQPQQRIPQPSQQRMLPPPPPPPAPVYNYSFQFNDPAVQYVGPPPPSHVGYVGYANPYYMSSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKD0</accession>
    <name>G3AKD0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Required for respiratory growth protein 9, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_49819</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for respiratory activity and maintenance and expression of the mitochondrial genome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRG9 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374402.1">
        <property type="nucleotide sequence ID" value="XM_007374340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKD0"/>
    <dbReference type="FunCoup" id="G3AKD0">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKD0"/>
    <dbReference type="GeneID" id="18871375"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49819"/>
    <dbReference type="eggNOG" id="ENOG502S7IA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1235709_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKD0"/>
    <dbReference type="OrthoDB" id="5578174at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010487">
        <property type="entry name" value="NGRN/Rrg9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13475">
        <property type="entry name" value="NEUGRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13475:SF3">
        <property type="entry name" value="NEUGRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06413">
        <property type="entry name" value="Neugrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="45"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="179"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW32887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="224" mass="26944" checksum="1E7911B01B87FC08" modified="2011-11-16" version="1">MLRRALDQSRLLALMYIRVGRTDISKFEKIQFSFSRCIHNRPTKEDLVKDNNNNIHPESNQPKPNSSEVPKEMHETTKLSFEEYQKKILLNHKKHAEPTEWQKRELAIKKRYGEWKPTKRLSREEMVKVKELSEQFPHYKTADLANIFRVSPEAIRRILKSNWIPNDTDLDKLTARRERQRQEKMEVVQQRYNKKSLKGPKQDNKRKSHKKVISSNYNYIGRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKI7</accession>
    <name>G3AKI7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_60931</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="9">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 24 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375107.1">
        <property type="nucleotide sequence ID" value="XM_007375045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKI7"/>
    <dbReference type="STRING" id="619300.G3AKI7"/>
    <dbReference type="GeneID" id="18873640"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60931"/>
    <dbReference type="eggNOG" id="KOG3196">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054362_1_0_1"/>
    <dbReference type="InParanoid" id="G3AKI7"/>
    <dbReference type="OMA" id="MYDTKPV"/>
    <dbReference type="OrthoDB" id="10254187at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902494">
        <property type="term" value="C:catalytic complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098796">
        <property type="term" value="C:membrane protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03064">
        <property type="entry name" value="TRX_Fd_NuoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000022">
        <property type="entry name" value="NADH dehydrogenase flavoprotein 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1590:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1590">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002023">
        <property type="entry name" value="NuoE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042128">
        <property type="entry name" value="NuoE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041921">
        <property type="entry name" value="NuoE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01958">
        <property type="entry name" value="nuoE_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005725">
        <property type="entry name" value="PRK07539.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10371:SF3">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10371">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE FLAVOPROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01257">
        <property type="entry name" value="2Fe-2S_thioredx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000216">
        <property type="entry name" value="NADH_DH_24kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01099">
        <property type="entry name" value="COMPLEX1_24K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000216-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="238" mass="26804" checksum="39CB6849D54B187B" modified="2011-11-16" version="1">MLSRYISKSVGQVSTVAKRYSSIISVHRDTTEDNQNIPFEFNSENLKRAQEIIAKYPPQYKKGACMPLLDLGQRQLGFTSISVMNYVAKMLDMPPMRVYEVATFYTMYNRKPMGKYNLQVCTTTPCQLCGSDGIMKAVRDHLQIKPGQTTPDKLFTLQEVECLGACVNAPMIAINDDYYEDLTPEATVDLLKQLQAGKELTKIGPVLNKRESCEPFSGPKVLLGKEPTDIRKFTRADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKR4</accession>
    <name>G3AKR4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Succinyl-CoA:3-ketoacid-coenzyme A transferase</fullName>
            <ecNumber evidence="3">2.8.3.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_135604</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 3-oxo acid + succinyl-CoA = a 3-oxoacyl-CoA + succinate</text>
            <dbReference type="Rhea" id="RHEA:24564"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:35973"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="2.8.3.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Ketone metabolism; succinyl-CoA degradation; acetoacetyl-CoA from succinyl-CoA: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the 3-oxoacid CoA-transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.3.5" evidence="3"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374483.1">
        <property type="nucleotide sequence ID" value="XM_007374421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKR4"/>
    <dbReference type="STRING" id="619300.G3AKR4"/>
    <dbReference type="GeneID" id="18869904"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135604"/>
    <dbReference type="eggNOG" id="KOG3822">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019942_1_2_1"/>
    <dbReference type="InParanoid" id="G3AKR4"/>
    <dbReference type="OMA" id="LGNCWFR"/>
    <dbReference type="OrthoDB" id="1933379at2759"/>
    <dbReference type="UniPathway" id="UPA00929">
        <property type="reaction ID" value="UER00894"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008260">
        <property type="term" value="F:succinyl-CoA:3-oxo-acid CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046952">
        <property type="term" value="P:ketone body catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1080.10:FF:000001">
        <property type="entry name" value="Succinyl-coa:3-ketoacid-coenzyme a transferase subunit b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.10">
        <property type="entry name" value="Glutaconate Coenzyme A-transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012792">
        <property type="entry name" value="3-oxoacid_CoA-transf_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012791">
        <property type="entry name" value="3-oxoacid_CoA-transf_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014388">
        <property type="entry name" value="3-oxoacid_CoA-transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004165">
        <property type="entry name" value="CoA_trans_fam_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004164">
        <property type="entry name" value="CoA_transf_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02429">
        <property type="entry name" value="pcaI_scoA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02428">
        <property type="entry name" value="pcaJ_scoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707">
        <property type="entry name" value="KETOACID-COENZYME A TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707:SF23">
        <property type="entry name" value="SUCCINYL-COA:3-KETOACID-COENZYME A TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01144">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000858">
        <property type="entry name" value="SCOT-t"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00882">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01274">
        <property type="entry name" value="COA_TRANSF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="5-glutamyl coenzyme A thioester intermediate" evidence="4">
        <location>
            <position position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000858-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="498" mass="53607" checksum="B7B72563996E374C" modified="2011-11-16" version="1">MLSRFTLSRSFAVRYLSTTPSKVVSTTDEALAGIKSNITLLSGGFGLSGVPDTIINALVKKPEINNITAVSNNAGLDGRGLSQLLVTGQITKMISSYIGGNRTFEKLYLTGKIDLELTPQGNLAERVRAGGAGIPAFYSPAGVGTWLEEGKLPVRYDETGEKVLKTSEAREVREFGGRKFLLEPAIFGDVALVKAYKADKLGNCWFRGAARNFNSAMGRNAKLTIVEAEHIVEPGEIAPEDVHLPAIYVDRVIQSTTPKDIEIFKFSEEKSEEPVEQSEADLKRAKIVKRAAQEFTDGSFANLGIGMPTLAPNYLPSDIHVTLQSENGILGLGPYPQKGQEDPDMINAGKETVTLAPGASLFGSEESFAMIRGGKIDLTILGGLQVSAKGDLANWGLPGRVKGMGGAMDLVSNPKATRVVVVMEHVDKKGRPKILEECKFPLTGKECVSRIITDLAVFDIVDGKLVLIEIDPASSVEEITEKTGAKFEVSPNLKKIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALA8</accession>
    <name>G3ALA8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_60454</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374666.1">
        <property type="nucleotide sequence ID" value="XM_007374604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALA8"/>
    <dbReference type="GeneID" id="18873422"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60454"/>
    <dbReference type="HOGENOM" id="CLU_3070174_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALA8"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="53" mass="6094" checksum="7C1343AD59E59DC2" modified="2011-11-16" version="1">MKNDYIIDHPQLSKKERVNGTETPAEIFLIPLDFITFFSADYGGCFLVRLLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALH6</accession>
    <name>G3ALH6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Diphthine--ammonia ligase</fullName>
            <ecNumber evidence="1">6.3.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Diphthamide synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Diphthamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_60556</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>diphthine-[translation elongation factor 2] + NH4(+) + ATP = diphthamide-[translation elongation factor 2] + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19753"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="Rhea" id="RHEA-COMP:10174"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16692"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.14"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.3.1.14" evidence="1"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374734.1">
        <property type="nucleotide sequence ID" value="XM_007374672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALH6"/>
    <dbReference type="STRING" id="619300.G3ALH6"/>
    <dbReference type="GeneID" id="18873461"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60556"/>
    <dbReference type="eggNOG" id="KOG2316">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2020848_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALH6"/>
    <dbReference type="OMA" id="LMREMCV"/>
    <dbReference type="OrthoDB" id="686384at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017178">
        <property type="term" value="F:diphthine-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01994">
        <property type="entry name" value="AANH_PF0828-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000145">
        <property type="entry name" value="ATP-binding domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002761">
        <property type="entry name" value="Diphthami_syn_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030662">
        <property type="entry name" value="DPH6/MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00290">
        <property type="entry name" value="MJ0570_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196:SF2">
        <property type="entry name" value="DIPHTHINE--AMMONIA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196">
        <property type="entry name" value="DOMAIN OF UNKNOWN FUNCTION 71 DUF71 -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01902">
        <property type="entry name" value="Diphthami_syn_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diphthamide synthase" evidence="6">
        <location>
            <begin position="40"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00029814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="123" mass="14053" checksum="835AF4EE25D3341F" modified="2011-11-16" version="1" fragment="single">MFQTVGHDIIEYYSQCLDVPLYRQAITGNSANQNLEYSPTDNDEIEDLFLLLSNIKQQHPEIEGVSCGAILSHYQRTRVENVCERLGLTSLTYLWQRGQDELMREMCVAQMDARIIKVAAIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALQ7</accession>
    <name>G3ALQ7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_60640</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374815.1">
        <property type="nucleotide sequence ID" value="XM_007374753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALQ7"/>
    <dbReference type="FunCoup" id="G3ALQ7">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALQ7"/>
    <dbReference type="GeneID" id="18873501"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60640"/>
    <dbReference type="eggNOG" id="KOG1693">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066963_4_2_1"/>
    <dbReference type="InParanoid" id="G3ALQ7"/>
    <dbReference type="OMA" id="VGEYTFC"/>
    <dbReference type="OrthoDB" id="1929172at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442598" description="GOLD domain-containing protein" evidence="10">
        <location>
            <begin position="19"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="180"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="11">
        <location>
            <begin position="30"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW33300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="214" mass="24490" checksum="C4DAE851985C38D1" modified="2011-11-16" version="1">MLVGFILTLLGLSQLIAGSAFTFILGAQEKSCYYVFTKRESTSIRYYFAVQSGGSFDVDYVITDPDGNVILSDQKQRQGDYVFTAHKVGEYEFCFSNEMSTFAEKVVDFEITFDNEDDANGFKANMPEQPNEKPLAHVENMQSTVNKIEQQLDGLYKTLQYYKTRNNRNQATVRSTESRIYYFSIFEVALMVGMAFLQITIVQLFFKGSRKQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALY1</accession>
    <name>G3ALY1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GTP-binding protein RHO3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_60670</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374849.1">
        <property type="nucleotide sequence ID" value="XM_007374787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALY1"/>
    <dbReference type="FunCoup" id="G3ALY1">
        <property type="interactions" value="116"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALY1"/>
    <dbReference type="GeneID" id="18873517"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60670"/>
    <dbReference type="eggNOG" id="KOG0393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_21_2_1"/>
    <dbReference type="InParanoid" id="G3ALY1"/>
    <dbReference type="OMA" id="THTIMLC"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030950">
        <property type="term" value="P:establishment or maintenance of actin cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045921">
        <property type="term" value="P:positive regulation of exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090338">
        <property type="term" value="P:positive regulation of formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010590">
        <property type="term" value="P:regulation of septum digestion after cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000780">
        <property type="entry name" value="Rho GTPase Rho3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
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        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="225" mass="24941" checksum="E44367862D2D5F56" modified="2011-11-16" version="1">MGLCGGSQKTIQRKIVILGDGACGKTSLLNVFTRGYFPQVYEPTVFENYVHDIFIDGQSVQLSLWDTAGQEEFDRLRSLSYSDTHCIMLCFSVDSPDSLENVQSKWVGEIADHCEGVKLVLVALKCDLRNNEDSEDFRASTAYNTHSSNPYGNNSNIYQQQKRSISYDEGLAVAKRIGALRYLECSAKKNRGVNEAFSEAARCALNAKPKGANDNEPDKKGCVVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMG0</accession>
    <name>G3AMG0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_54822</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374327.1">
        <property type="nucleotide sequence ID" value="XM_007374265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMG0"/>
    <dbReference type="FunCoup" id="G3AMG0">
        <property type="interactions" value="257"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMG0"/>
    <dbReference type="GeneID" id="18871893"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54822"/>
    <dbReference type="eggNOG" id="KOG3385">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086133_1_1_1"/>
    <dbReference type="InParanoid" id="G3AMG0"/>
    <dbReference type="OMA" id="MNRMLIM"/>
    <dbReference type="OrthoDB" id="261831at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd15853">
        <property type="entry name" value="SNARE_Bet1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039899">
        <property type="entry name" value="BET1_SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="48"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="138" mass="15801" checksum="619D8D9002A6F43B" modified="2011-11-16" version="1">MSRYSADQQDLRTQLFSTTTTRRVPNRTASPYDPIPAPSAKHSESMLSSLESQNNDDVNVMNEKVAMLKNLGVKIGVEINKGIKLNDDITNSFEKGSVNLKNTFNHMVVMSKRAGITWKMWLLVFTIVGIWFFWIWLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMG1</accession>
    <name>G3AMG1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_49756</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374328.1">
        <property type="nucleotide sequence ID" value="XM_007374266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMG1"/>
    <dbReference type="STRING" id="619300.G3AMG1"/>
    <dbReference type="GeneID" id="18871369"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49756"/>
    <dbReference type="eggNOG" id="ENOG502S0U7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_443428_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMG1"/>
    <dbReference type="OMA" id="SSIAKYR"/>
    <dbReference type="OrthoDB" id="4094935at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="369"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="423"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="517" mass="57772" checksum="81C05672B9B26C7A" modified="2011-11-16" version="1">MTNTPQFKSPPLPSIESFKLDNILNTSLANESNQVISDLLNITNNYQQDLAQGIKTNLIIEQKIQSKNVEVVKLAHGISSQLKSRVKKLNKVTGESDEVNKLLRNTVIVSDKVRSLVGQLHEIEMLVNGSVNPSVYPNIYKILQKQQRLQAVDQSQVEPRPSVESPTPARLSPTPPHAPIPLRAAEAPTPPPPPPPPPPKSPEQSLSESESSEIIPYSPELPSRQPPETAKDIPQSPEPEDMDPDQFELFMSESISKYRHRQSLKHKQLPQSPPPINNPLNLLYSQLIPKPSQQFLKSPFVNSLPMKSMPIIKPTLQTSHFKKLRINGDPIGSTKHDGCGCNSPDHSEQELVFQDDEELDWKGLSCDEELTETDSSESDSDDDTTSLPMMTNQYYKNLKRASRKRTRNPPLPPARQPELSPTPKHKPSHHTLKPKRSILKTSDRIVFSKENSPYRQAVKATNIIPDAVAIVNNKYASRIEYPVSEFTAEGVIIEPETVEADDSSSIRSISVLKSYIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMH9</accession>
    <name>G3AMH9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_137304</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374346.1">
        <property type="nucleotide sequence ID" value="XM_007374284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMH9"/>
    <dbReference type="STRING" id="619300.G3AMH9"/>
    <dbReference type="GeneID" id="18870038"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137304"/>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_468501_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMH9"/>
    <dbReference type="OMA" id="LHLCFQY"/>
    <dbReference type="OrthoDB" id="823504at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="117"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="268"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="352"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="269"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="288"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="305"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="362"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="381"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="392"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="432"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="463"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="487"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="514"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="538"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="563" mass="63016" checksum="F192DA11F89F49F9" modified="2011-11-16" version="1">MLDPQQRLRKAVIDGNLPIVQRLLSRFPSLWLNVDSAHDGWSNLHYASYYGHYLVCFHLVSFINNDLESKYTKLDLLSFDNLTVLHVCIEGKHLQTLHYLLQEFPGKLWLNYPGGASKQTPLHWSCKYGFSEGTKLLLEFGASWDERDGNGDTCLHHCFQHGNTQCIQEILRYIFQNTNDKEKAIDKIDYFERMRNKRGCVAFDYASSSSLARKYQLLKQDLMALDFSDSGEASSGSSMVYTLHQNQSMHSLETSRILTSPILPMGIQKQEDNEHTIIHDDIDTTITTEEEEEEEEEKPGRRSHSQSLPTNPETTNSLPGTKVRSRSNTNYIYHPPNLNNIPVLGSPRFASPPVMPPPSTPITNRAPSLKSVTISPSVRSNGHDSTEDSQRPESPQSIWSLQSTNTSPAKSQHGQRRSFSFSKSSMPRPRAESASAVANSASVAAKKAFVSSRPSHENHRSRPNTPSQRSRQSESSHTSPTLQKLNSRTSSTSIGSIGSQTSPTSQHEHILRKSQSSSTLSSDPTTIPIIRQPPQRTDSAGSLTMSDSSRKFSVNSISFSRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AML3</accession>
    <name>G3AML3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DDHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_71305</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374971.1">
        <property type="nucleotide sequence ID" value="XM_007374909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AML3"/>
    <dbReference type="FunCoup" id="G3AML3">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AML3"/>
    <dbReference type="GeneID" id="18875373"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_71305"/>
    <dbReference type="eggNOG" id="KOG2308">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007365_0_0_1"/>
    <dbReference type="InParanoid" id="G3AML3"/>
    <dbReference type="OMA" id="HIQRGYS"/>
    <dbReference type="OrthoDB" id="69269at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008970">
        <property type="term" value="F:phospholipase A1 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032048">
        <property type="term" value="P:cardiolipin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046337">
        <property type="term" value="P:phosphatidylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004177">
        <property type="entry name" value="DDHD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058055">
        <property type="entry name" value="PA-PLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055555">
        <property type="entry name" value="PA-PLA1_DUF7131"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057826">
        <property type="entry name" value="WWE_C20G8.02"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509:SF10">
        <property type="entry name" value="LD21067P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509">
        <property type="entry name" value="PA-PL1 PHOSPHOLIPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02862">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23465">
        <property type="entry name" value="DUF7131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23463">
        <property type="entry name" value="WWE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01127">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51043">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDHD" evidence="2">
        <location>
            <begin position="476"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="592"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="709" mass="80969" checksum="0B0CAD490C97BB14" modified="2011-11-16" version="1">MRWFYAVDVPNSKPGWNKYTKTKEPEKFIPFSEWDSFNLEVNFRKKSTVPVEVNEDRLFEVDLQKLELAPVYWEGPIYEVRRGVWFDSNGIPFSKKQTEELEQGYKKVKPYTFNHEKPDTESKENKEIISKFNKYIKEVEDSTQKVDLSQEKDILKLESGNYVMYLDGKNAVTFPADYDTNFQIDVIRQFSSTSTVPLIGVNSIQRGYSDDMGESIFDKLSTNAIPTIADSFQDEFGSILGFDQTITRENDETETKTETENEIDDHHMQQYLEGDYDHSVSKNESNREIDHLIFCIHGIGQVLGKKYESINFTHNINVLRNTMKKVYTENDQYKKLAYPIADKKDETYNTNNRIQVLPITWRHKIGFNPEETVKEHDPRLPTLSQINVDGIRALRNVVGDVVLDVLLYYEPKYLKEILMATSTELNRVYKLYKERNPNFNGRIHVLGHSLGSAIAFDLLSGQTGTAHGDIELPNKLDFQVDSLFLAGSPVGLFKLLEGKNITAAFDDKVVNNNGKDLDKVAAPNCRNLYNIFHPCDPVAYRIEPLINPQFGNFKAEQIAFASRGISTHIQELYSYGDGITGKISQATKWLRGATKSEPSPQSKSIEESASEENALGDIITSIIVDEKKQESNKVTKRNMTDDELIELLALNNTGRVDYCLPMGVLDFSLVSAVSAHISYFENQDTSGFIMKELLEGRDDPVRKKFVSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMM2</accession>
    <name>G3AMM2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Gdp-GTP exchange protein for the Rho1p small GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_137544</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374981.1">
        <property type="nucleotide sequence ID" value="XM_007374919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMM2"/>
    <dbReference type="FunCoup" id="G3AMM2">
        <property type="interactions" value="343"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMM2"/>
    <dbReference type="GeneID" id="18870052"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137544"/>
    <dbReference type="eggNOG" id="KOG4305">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001251_2_1_1"/>
    <dbReference type="InParanoid" id="G3AMM2"/>
    <dbReference type="OMA" id="CFRQKII"/>
    <dbReference type="OrthoDB" id="2272012at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04435">
        <property type="entry name" value="DEP_fRom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041675">
        <property type="entry name" value="PH_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052233">
        <property type="entry name" value="Rho-type_GEFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572">
        <property type="entry name" value="RHO1 GDP-GTP EXCHANGE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572:SF2">
        <property type="entry name" value="RHO1 GDP-GTP EXCHANGE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15405">
        <property type="entry name" value="PH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="4">
        <location>
            <begin position="226"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="5">
        <location>
            <begin position="609"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW33466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="927" mass="106099" checksum="AE08A37313AB5B78" modified="2011-11-16" version="1">MTKLSSLSTKKGSSTSIANQKLEKHQSISSTKTLVHPSHGPTVYPALLSEVAKVFKEQMPRDTRFTKDGLDYYGSFTGNMAVDVIARIIRTNDRNLALLLGRSLDAQKFFHDVSYDHRLRDSANELYEFSHVYNGELTASQETGVNGVFTILTDCYSPTCTRNHLCYSIACPRRLEQQARLNLKPQGGLKRAVSRLSLHEQEESETLWHKTVPQSVLDKLDKQEKTRQELIYEFVYTERDYVKDLEFMEDFYIRKLSVLPIIAENQREAFLRTVFGGVHELLALGRSFSEALTRRQQQQKPVVETIADVFLDYVGKFDPFVIYSGNKVFATFEHERQQQVNPRYSAFLDKVERQAESRRQDLSSFLIKGVQRPARYQLLLAGILKNTKPDSPDYKNLVKAKEEIEKVLAKINVQTGISTDRHKVMVLHRLLGRQTLENRFNFRLKYSDRIIYQATLSRKRDNEKIDLYIFEHALLLVKHKIQNKREQHKVLERPFLLPLLFVNSGYEIPTMRTIMNTRYHNSLLSDVSLKSQRAETNYIGNTLNSSSNAKFQLNFYALGSNPVHLSLYAEDAAIQNQILQQVSQQQKKLIDNSDLFSLTKYETRKFQGNNRINCAVPCYGGKKLLYGTDSGVWVSTVRSISTTSNEKICSDPTLVISKVYVTQIEVLVEFNKLLVLSDKSLYEFDLNCTDSLDHVKNTKSGKLLMTHISFFKIGICDGRLIVCGARTGGQHSICMFEPASPHDKNQRNKSKKLEVQEISFSSDPISISFLKSKLCIGCAKGFEIVTPDGKTKESILDEADPSLDFATQRESVTPLAIYRVGKDFLLCYAEFAFLINRNGWRTTHDWEIFWQGNPQNIALFFPHILAFEPGFIEIRDLTTQTLLRALAGENIRFLHSNEHEAMFACEENGYDIIVSIDFLNLKPKSPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMS9</accession>
    <name>G3AMS9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60869</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375038.1">
        <property type="nucleotide sequence ID" value="XM_007374976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMS9"/>
    <dbReference type="FunCoup" id="G3AMS9">
        <property type="interactions" value="545"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMS9"/>
    <dbReference type="GeneID" id="18873608"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60869"/>
    <dbReference type="eggNOG" id="KOG0653">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020695_11_0_1"/>
    <dbReference type="InParanoid" id="G3AMS9"/>
    <dbReference type="OMA" id="DYKFIGM"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044772">
        <property type="term" value="P:mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20512">
        <property type="entry name" value="CYCLIN_CLBs_yeast_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000001">
        <property type="entry name" value="G2/mitotic-specific cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046965">
        <property type="entry name" value="Cyclin_A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001771">
        <property type="entry name" value="Cyclin_A_B_D_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 4" id="KW-0195">Cyclin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="241"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="7">
        <location>
            <begin position="334"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="338"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="22"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="90"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="35"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="475" mass="54828" checksum="0F881EC5FD4ADF91" modified="2011-11-16" version="1">MVQTTVNNENEFRLTRSKLLLQQQQQQQQQQQQQENEAQHQSVFASSQPDGASKPSSESSTQELAPTRQYLGDVSNQYITTKQSTTTLITTTTQQQSKSQPLVKKRKPLVPLHEQPKPSKDISIASDSANASTSYLAVPSRLPQKRQATESSTNLVEQLKVPPQSEAVATNTNNVNKKTRLIDYEWQDLDEEDLDDPLMASEYVNDIFSYFYELEQRMLPDPQYLFQQKHLKPKMRSILVDWLVEMHLKFRLLPESLYLAINIMDRFMSIEVVQIDKLQLLATGSLFIAAKYEEVFSPSVKNYAYFTDGSYTEEEILQAEKYILTILNFDLNYPNPMNFLRRISKADDYDVQSRTLGKYLLEITVIDYKFIGMRPSLCCASAMYLARLILGKSPVWNGNLIHYSGGYRLSDMKTCVDLIFQYLISPIEHDEFYKKYSARKFMKASLLCRSWAKKFHQEGKTLLDEKTSTHRLALT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMT8</accession>
    <name>G3AMT8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_60876</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S11 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375047.1">
        <property type="nucleotide sequence ID" value="XM_007374985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMT8"/>
    <dbReference type="FunCoup" id="G3AMT8">
        <property type="interactions" value="1393"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMT8"/>
    <dbReference type="GeneID" id="18873613"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60876"/>
    <dbReference type="eggNOG" id="KOG2908">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042989_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMT8"/>
    <dbReference type="OMA" id="SFEDYWE"/>
    <dbReference type="OrthoDB" id="1093at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054179">
        <property type="entry name" value="PSD13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035298">
        <property type="entry name" value="PSMD13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10539">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10539:SF0">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22037">
        <property type="entry name" value="PSD13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="194"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="405" mass="47521" checksum="32E7D9428E6FFAB2" modified="2011-11-16" version="1">MDLDNDVSTILAILRSETESPELINVLYQLEDFYERKLWHQLTQVLDEFYYQFDKSLVTPTLKFKIYSLFITQFQSKLNPIKVVDFLLESFSKPEETSDKLLELKQEFLTQIKKENNFKDDDEEFKKVVDEDESIIYVDLQIARYYLILSRLNDSEDILSRLSPKFESTTNDLNSKINAAYYLTKCEYCQITENYNDYYKNGLLYLSSVPNLTEEDKVKICYQLCIAALLGDRIYNFGELILHDILSTIESESSQYNWLYHLIQDLNSGNLQEFNKWLSVGFQRSPMLVKFELFLKQKIIIMALLELISQTPTTNKQLTFQEISDFTSTPLNDVEHLIIKCFSLNLIQGYINQIDQVLNITWLQPRILNLTQVKTLYNHLVDWDNKVDNLAKTVYQNGGTVWAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMV1</accession>
    <name>G3AMV1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_61446</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375641.1">
        <property type="nucleotide sequence ID" value="XM_007375579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMV1"/>
    <dbReference type="STRING" id="619300.G3AMV1"/>
    <dbReference type="GeneID" id="18873897"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61446"/>
    <dbReference type="eggNOG" id="KOG0601">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_874947_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMV1"/>
    <dbReference type="OMA" id="WMIDPNP"/>
    <dbReference type="OrthoDB" id="5337378at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110031">
        <property type="term" value="P:negative regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF196">
        <property type="entry name" value="MITOSIS INHIBITOR PROTEIN KINASE SWE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="200"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="306" mass="33551" checksum="38726724C86AE253" modified="2011-11-16" version="1">MTEFCEGGTLFDFLEENKHYRIDEFRIWKILIEILSGLRFIHSKNYLHLDLKPANIFITFEGSLKIGDFGLATKLPILEKDFDLEGDRNYIAPELINDKIYTPFADIFCLGLIILEIAANIILPDNGTPWRKLRSGDLSDAGQLSSDNISMFLHHHASSAGGCGGESNHCLSSSTTNISSTHSLTINSSASNLFTMPPQNPVNNSTTQLNPPATAITSGTPATTGSSSGMSNKASARDLIPPWAPEFLTSGDSMNLDRLVNKMLRPNPFDRPSASVILEMEECVIIENRRKAGATIFEGEFGPPDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN59</accession>
    <name>G3AN59_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Threonine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Threonyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_61003</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="3"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375178.1">
        <property type="nucleotide sequence ID" value="XM_007375116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN59"/>
    <dbReference type="FunCoup" id="G3AN59">
        <property type="interactions" value="1014"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN59"/>
    <dbReference type="GeneID" id="18873676"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61003"/>
    <dbReference type="eggNOG" id="KOG1637">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008554_0_2_1"/>
    <dbReference type="InParanoid" id="G3AN59"/>
    <dbReference type="OMA" id="WYADGMY"/>
    <dbReference type="OrthoDB" id="5423599at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01667">
        <property type="entry name" value="TGS_ThrRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000009">
        <property type="entry name" value="Threonine--tRNA ligase 2, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000003">
        <property type="entry name" value="Threonine--tRNA ligase 2, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000006">
        <property type="entry name" value="Threonine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000005">
        <property type="entry name" value="Threonyl-tRNA synthetase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF46">
        <property type="entry name" value="THREONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGS" evidence="15">
        <location>
            <begin position="72"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="14">
        <location>
            <begin position="304"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00031900"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW31902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="720" mass="83014" checksum="D46B876A422C2BDA" modified="2011-11-16" version="1">MSESLPEQVEKLSIDKKQTDAPVASKKKEKKAAPSKNPLLLDPQPAFIDSRIKLFDELKAKYDEEIASKPKVAIQITLKDGTIKEGVSYETSPLDIANSIGKSFAERQVIAKVNGTLWDLPRPLEGDVKLEFLDFEHPEGKAVFWHSSAHILGEACECHYGSHLSHGPPTEDGFFYDMSINDGATVVSQSDFGNLEQVATKAIKEKQKFERLEMTKEDLLRMFEYNKYKVKFISDKIPDGTSTTVYRCGPLIDLCVGPHIPHSGRIKAFKVLKNSSSYFLGDASNDSLQRVYGISFPDKKLMTEHLKFLAEAAERDHRKIGREQELFLFNEMSPGSAMWLPHGTRIYNTLCDMLRKEYRLRGYEEVITPNMYSTKLWETSGHWQNYKENMFSFEVEKETFGLKPMNCPGHCLLFKSRERSYRELPWRVADFGVIHRNEFSGALSGLTRVRRFQQDDAHIFCTFDQIEQEISGVFDFLQKVYGIFGFEFKMELSTRPEKFVGDIETWNSAEAKLENSLNKFLGQGKWELNPGDGAFYGPKIDIMISDALKRWFQCATIQLDFQLPQRFDLEYKSENTNERPVMIHRAILGSVERMTAILTEHYKGKWPFWLSPRQILIVPVGPKYYEYGQKLQKKLNDEFFFYADVDVSGNTLSKKVRTGQIYKYNFIFIVGEEEETSESVNVRNRDIPEDQGKNAMVKFDEVVQQLVKLREEKRSDNKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANB9</accession>
    <name>G3ANB9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_138015</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375778.1">
        <property type="nucleotide sequence ID" value="XM_007375716.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="18870091"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138015"/>
    <dbReference type="eggNOG" id="ENOG502RQ30">
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    </dbReference>
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    <dbReference type="InParanoid" id="G3ANB9"/>
    <dbReference type="OMA" id="YEAKFRI"/>
    <dbReference type="OrthoDB" id="2253354at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035213">
        <property type="entry name" value="DUF5321"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17254">
        <property type="entry name" value="DUF5321"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="159" mass="18510" checksum="5AEA71B21AFE27C6" modified="2011-11-16" version="1">MFRLVRQRTTGNACRYQSTTASRYIRAKPRAIDMFRTPTFKSIVLTLVFCSIVVDVTKTRKELEGLNNSYNSKFELLNDIIDKLNANQPIDLAKELKMANLLTKHKYKSVVDIELDEQLEDMFKELQEEAEQEPVQQQAPEQEQQQPEGKPKKNTNKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANK0</accession>
    <name>G3ANK0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vacuolar ATPase assembly integral membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_61593</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375805.1">
        <property type="nucleotide sequence ID" value="XM_007375743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANK0"/>
    <dbReference type="FunCoup" id="G3ANK0">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="GeneID" id="18873967"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61593"/>
    <dbReference type="eggNOG" id="ENOG502RZXR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091774_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANK0"/>
    <dbReference type="OMA" id="WYWTGSS"/>
    <dbReference type="OrthoDB" id="19981at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990871">
        <property type="term" value="C:Vma12-Vma22 assembly complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021013">
        <property type="entry name" value="ATPase_Vma12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31394">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31394:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11712">
        <property type="entry name" value="Vma12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="25186" checksum="761C3BA49CA4101E" modified="2011-11-16" version="1">MTRYQTTPAFKELIESASNIPSSLKSRLLHSDSLVTHTDLIEFYKTCKPTDTLLDLIKLTKLDIPNKNISPNKPKTKEYLALMERLRLEEKEREYKRLITPSPEYETLYDNTLMGDEEQMSVAQMHKELKHQLTTIVNILISVGSVAYAIWYWTESSWGLPVSYRLLLSIFFGLLVLIAEVVVYMGYLNKIEDARVRERKKKEVKKVIRTVDLKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANQ6</accession>
    <name>G3ANQ6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_61103</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375267.1">
        <property type="nucleotide sequence ID" value="XM_007375205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANQ6"/>
    <dbReference type="FunCoup" id="G3ANQ6">
        <property type="interactions" value="1314"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANQ6"/>
    <dbReference type="GeneID" id="18873712"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61103"/>
    <dbReference type="eggNOG" id="KOG2210">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012334_1_1_1"/>
    <dbReference type="InParanoid" id="G3ANQ6"/>
    <dbReference type="OMA" id="AMYISEQ"/>
    <dbReference type="OrthoDB" id="14833at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000007">
        <property type="entry name" value="Oxysterol binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2720:FF:000001">
        <property type="entry name" value="Oxysterol-binding OBPalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF102">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW31991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="34452" checksum="0C83D6253688592E" modified="2011-11-16" version="1" fragment="single">MGLTSKLDKLKLLGSAEEHISNDRKNTSTQNSDDIDEVDNEGQNILLGIIAQLRPGMDLTKITLPTFILEKKSMLERITNFFQIPHLLLEANNTADPLQRFIGILRWYLASWHIAPKAVKKPLNPVLGEVFSCYWDNLEDGKSAYYIAEQTSHHPPKSSYFYLIPSEKLRVDGVVIPKSRFLGNSSAAIMEGSGHVTLGKWEDELYVMNQPNVYCRGILFGTMRTELGDRMHVKCAKTGFEAEIEFKTKGFISGTYDAIEGIVKNSETSEHLYQVSGKWNDVMEIRDLKTGKKQILFDTNKSSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANT2</accession>
    <name>G3ANT2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61118</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375293.1">
        <property type="nucleotide sequence ID" value="XM_007375231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANT2"/>
    <dbReference type="GeneID" id="18873723"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61118"/>
    <dbReference type="eggNOG" id="ENOG502T6HQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1171245_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANT2"/>
    <dbReference type="OrthoDB" id="4028164at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="237" mass="25483" checksum="C0DAB139DF2C7354" modified="2011-11-16" version="1">MTTDDINKATRIWSIIDGKLDLTRPTKRALVEFIICNDGSVYIGGSDVQCEIYPVQAENVEESSSSSESGTTVVQSTDEPQPTVAPGTEEPQTTVAPGTEEPQPADSSATHSGTDTEDGEVTEYTTITTDITITITDCDESDSCSTTESVKKTTIETYYPITVADNVHTITLTSCVEEICIEVTKVIGHHHHYHDTVSHLVANPTNAVPVPYVANSGNSAYVKNLFAVVAMIVPMLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANY3</accession>
    <name>G3ANY3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_153747</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375884.1">
        <property type="nucleotide sequence ID" value="XM_007375822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANY3"/>
    <dbReference type="GeneID" id="18870963"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_153747"/>
    <dbReference type="eggNOG" id="ENOG502RQ7Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111193_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANY3"/>
    <dbReference type="OMA" id="YIQCTIR"/>
    <dbReference type="OrthoDB" id="4084909at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="195" mass="22928" checksum="E166B970D6134FBB" modified="2011-11-16" version="1">MDKALTELSNRIGNLELLLNHTNQNHSKLFQVESLKQQLATVYKEHPELVKFNQILTDLKLWDALKKQDSSEEDVSREMKQEFIVFNYSTLKEITSNLAELSSLNSTTVIDKLNTDIQTTDISSSKLLLSTYKDDIFRINELMTMLIMKNMIVYEEYVQLMIKENKKWLQFEEMLSQLESKINHIETIQAQKNKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANZ6</accession>
    <name>G3ANZ6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial group I intron splicing factor CCM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_138987</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375897.1">
        <property type="nucleotide sequence ID" value="XM_007375835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANZ6"/>
    <dbReference type="STRING" id="619300.G3ANZ6"/>
    <dbReference type="GeneID" id="18870166"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138987"/>
    <dbReference type="eggNOG" id="ENOG502QVB0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_434755_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANZ6"/>
    <dbReference type="OMA" id="SFEQYIY"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="651" mass="75671" checksum="314FB13CE0075ACA" modified="2011-11-16" version="1">MLSIKDTISRIGRVIRHSQILKDSENKAIVKNSSNSKVNPQSTTNSYSPKVIQSIYKNLQPDDVKKFKPLYQDFQRKTYREFLEPLDMPTLKTFSEKTEYDIRSAKQAWTKKTSKNPRAILEQYMEDRRMFEIMMSTLIDLTPSHLKDIDHNNPAVLNNVILQQDQIASEYKLQVPAHNFHEIPIMPSPLTRENFYEYIYQLTHTNYHYKNSSSLQSGIIPQILLYTHKLTTHEFKEYRSTHTYNCLIHYFGSLKNQSTFARELLLVMKNDGHAYNVDTINSMIKMLSTHANIRGNTSTFGLVLKYLQLSARLNIEINLGTYSYIYSTIKNIHLKENFLKKMQDHGIPIPKSMMVKVLDDFAKTTTDYHEVINFIEKDLGYETWAIDSKFSNKVIYSQALHANANDAELEKFNIDEYTLLNLFSGIQRNVELQDKAFSMIRIYVEFSKESGRDFTKIPRIFQLISRQLITDLKDVRKIETLSYLLRCIIYEATQKLNLPIEVIDYGNGKRSLPENYKIVSRIVGRALQEVDAKITYVNENLKDVSIKPTSVLLDDQEIEIWNHLKLELEQPNSNKKEDLLLFKVIRSGQLPNGAAVELPHESIQQILSHTAAKISAHRNRERILRIDEGSDEYTKRQMVERGLMKNSGQST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP15</accession>
    <name>G3AP15_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_66712</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375322.1">
        <property type="nucleotide sequence ID" value="XM_007375260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP15"/>
    <dbReference type="GeneID" id="18875112"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66712"/>
    <dbReference type="HOGENOM" id="CLU_888938_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP15"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442537" evidence="1">
        <location>
            <begin position="17"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="313" mass="34328" checksum="8C170818EA5A209D" modified="2011-11-16" version="1">MIGYIALFALTALVLGETTEYPPAYKNTIAKNVGLPGNEYSTVNLSDYLNYGPSSTISIDSNEEYSSYPIDESSSYSSRIYSTVNDWANSSSIETNSYSTDTYVLSIGTTMNYRYSYPSSACNALTTETIYIEASETVVVTETTTTTVECEGTTTTVAVDGSGGWIEGNGSQGAMTTTVNRDTYTLTYTYTDATTTYTTIYTTEVYDTGYTAKYQNSTSGSNSRIGSSSSEYIESFMYDPYTTELSEYTTSIYYPTTSSEPEESSSMWVDSLASDYVYPSSSGYYPSVSDSEPESLSTAPYSAYYYKGSTIYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP34</accession>
    <name>G3AP34_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_61153</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375341.1">
        <property type="nucleotide sequence ID" value="XM_007375279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP34"/>
    <dbReference type="GeneID" id="18873747"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61153"/>
    <dbReference type="HOGENOM" id="CLU_2869066_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP34"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="64" mass="7042" checksum="D92B82DA777F4CDB" modified="2011-11-16" version="1">MLSGSGHVIKSVSGHYLALSFELLRSRRIISYAAWLHEEFFLVVYEHCIVALSCTISGNSGVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP91</accession>
    <name>G3AP91_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_61724</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375938.1">
        <property type="nucleotide sequence ID" value="XM_007375876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP91"/>
    <dbReference type="STRING" id="619300.G3AP91"/>
    <dbReference type="GeneID" id="18874023"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61724"/>
    <dbReference type="eggNOG" id="KOG0519">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008307_4_1_1"/>
    <dbReference type="InParanoid" id="G3AP91"/>
    <dbReference type="OrthoDB" id="21225at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036180">
        <property type="term" value="P:filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900445">
        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000146">
        <property type="entry name" value="Putative two-component response regulator SSK1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="231"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="14"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="55"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="161"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="179"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="3">
        <location>
            <position position="280"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW32662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="363" mass="40859" checksum="DF379133FACBA1BE" modified="2011-11-16" version="1" fragment="single">MTLQDALIIDVATDRLPSNFPPPPQARYSEPHYSMGNNSPRDPQFLKIHNHNVKHPQPIPIKQSPSQIYSNPYQQKYPKNYPYSVREKSVSPSSMLRNLPNHRRSQSNPLQSPLSAPVNSTETENPKAVLLLPKNFSLAGGSTGGGNNKKRNQSLYDNFGSEIKTDDDIKPNQSMLEPPEEKPDDEKEVYEQKEERPKTSRSPSKEQIETKKEKEKAKPDAAKDKVLPSISVLIVEDNAINQAILGAFLRKRKIHYQIAKNGQEAIDKWRSGGFHLVLMDIQLPVKSGIEATKEIRHLEKLNKIGVFDPNELSMIKNNEELKPEDKLDLNVFRSPVIIVALTASSNSSIDRKNALTAGCNDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3APH2</accession>
    <name>G3APH2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_152650</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375419.1">
        <property type="nucleotide sequence ID" value="XM_007375357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APH2"/>
    <dbReference type="FunCoup" id="G3APH2">
        <property type="interactions" value="464"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APH2"/>
    <dbReference type="GeneID" id="18870934"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_152650"/>
    <dbReference type="eggNOG" id="ENOG502QPVP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015609_0_0_1"/>
    <dbReference type="InParanoid" id="G3APH2"/>
    <dbReference type="OMA" id="MLWKDVD"/>
    <dbReference type="OrthoDB" id="2341546at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001278">
        <property type="term" value="P:positive regulation of L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.10:FF:000003">
        <property type="entry name" value="C6 transcription factor (Leu3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051089">
        <property type="entry name" value="prtT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845:SF21">
        <property type="entry name" value="REGULATORY PROTEIN LEU3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="89"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="31"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="205"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="733"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="824"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="45"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="228"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="733"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="767"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="825"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="852"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="887"/>
            <end position="911"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="961" mass="107932" checksum="340D8057BD08B495" modified="2011-11-16" version="1">MSNGNSSGPRPGTNSLGNLVNVATSISQELHLIDSQQIKSERQQHQPSPTPSNHNSSHNGPVPPLTSSLSPPLANSPASQKAGKPKRMACVECRQQKSRCDASERHPEPCTRCFKKGLKCDLKSDYKRTYKRARIAQIEREFSELKKTLNSTQAAELLNKVPSLANITSPQEFVSSPQSRTIIPNPSAIRHSQVWNNNINNNLHNPEQPHSALARPPIATPYDRPHHVSSSETNYSIPNTPSIARPVPEPTGYQGPVNRELQITMSEAAMKCEEKSVDSITLSPETIKALFLEYVERYHPIVPIVDVEMGPERIYRICPALFWVIMFVSLRRFYEDTSLLVQLSPIVKGILAEIMISPITRYNPFEEDEPTLNVSSVYSVQAFLLYSYWPPITSSLSADSSYSTVSTAFFQAIRIGLHSPVSMSDTNINSRTPQQLTMAKEQAKTWIVSNIASQTIATAFGFPACVQFDSSVWSYSRPDRGIKIPQAIQFMMEIAYFEDQMAKSLNSNPLDTYGLIDPTERLPLLKLLTKRLDELEIKMSHELPYEDAFRKFQLLSTRVHLLTYYFMDSSRIADFELQKGLVKLYNAAIALINHTRAYQAKDKKFIKYLPGVYILNVWQAACIIGKLIHSRLKSLVDTGSGKQSYEAAIALMAKASILKHDMAYRASAITRNMWQLFRTLDEKKMSSLSIDIRNRMSASVFFDCLYLLRTQVGMTKLNRDSNNIAEQIDHEQEEAVVSYDEDENSSGESSASEEAVEAEEKNEGNPSAKSTPGSTTSSSRIRKARSLSHTVDAESKARKIIMTIPLDPQPISVSGGKRSSIFKVVNSSNDSSPHVNNSSEKSSPNVTRQAESPNNSRLSSSNNNKPAPPSSWKPVENKEEVPVLPKQTQKQPQQYPQQQQQQQQQQQYVQDYQPQQQFSDMVFNESPIQLGLENLEMDTFDHDMLWKDVDSVMNDFGFPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3APY5</accession>
    <name>G3APY5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_138825</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375582.1">
        <property type="nucleotide sequence ID" value="XM_007375520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APY5"/>
    <dbReference type="GeneID" id="18870154"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138825"/>
    <dbReference type="eggNOG" id="ENOG502RQ1B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1001147_0_0_1"/>
    <dbReference type="InParanoid" id="G3APY5"/>
    <dbReference type="OMA" id="KTMVHPQ"/>
    <dbReference type="OrthoDB" id="4078061at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="274" mass="31069" checksum="3DA4C58D8FA59513" modified="2011-11-16" version="1">MGASASKPGRKLTKVVSETAKAKPLNRSTNVNPLPSQTLKDRFEQQPISQQSIPVEPTREETAPQMTVQTPQQDAQSPAFRSTSSFDPSFLSKKLNKQTVIPEGKDGGDPHVEGTDTYQRGFVDSVTKLGKHVQSVEYNPLMNKNVLALKQLQFRKTISEQGEKELESEKNNENGEEMFKTMVHPHTLTAILSDLKDPRMTPESITKDYQLHPDFLKELGDRFKIPTTRKPIEEEKPKEDEFISGVEVDSAMQFEEPESKVDRERFKKLKSRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3APZ1</accession>
    <name>G3APZ1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_61389</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375588.1">
        <property type="nucleotide sequence ID" value="XM_007375526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APZ1"/>
    <dbReference type="STRING" id="619300.G3APZ1"/>
    <dbReference type="GeneID" id="18873870"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61389"/>
    <dbReference type="eggNOG" id="ENOG502SCR7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1019408_0_0_1"/>
    <dbReference type="InParanoid" id="G3APZ1"/>
    <dbReference type="OMA" id="DTRNINH"/>
    <dbReference type="OrthoDB" id="5382468at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12418">
        <property type="entry name" value="RRM_Aly_REF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051229">
        <property type="entry name" value="ALYREF_mRNA_export"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965">
        <property type="entry name" value="RNA AND EXPORT FACTOR BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965:SF82">
        <property type="entry name" value="THO COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="82"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="170"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="177"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="220" mass="24270" checksum="67184ED898A7D3D6" modified="2011-11-16" version="1">MSDILEKSLDEIIGEKKKDPRRGPRPGPPRRFRGGHASFSRGGGAQHGKIHKQRPAGHHGHPKRLNYSSLPRDIASLAGSRPVLRVKNIHPDLNGEDLSQLFGNISPVDFVKFDAENDSNAYVCFHNDNAKNNAEAIEKYDGKKAMGKILIVENAVSLADRISSLPVRRQPVTPAALKHHKKPVGKGRRRSKPSVEDLDKELNAYMNADADQPSDAMNVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ20</accession>
    <name>G3AQ20_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_61419</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I 23 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375617.1">
        <property type="nucleotide sequence ID" value="XM_007375555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ20"/>
    <dbReference type="STRING" id="619300.G3AQ20"/>
    <dbReference type="GeneID" id="18873884"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61419"/>
    <dbReference type="eggNOG" id="KOG3256">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067218_5_1_1"/>
    <dbReference type="InParanoid" id="G3AQ20"/>
    <dbReference type="OMA" id="WRPVIDY"/>
    <dbReference type="OrthoDB" id="204405at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3270:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit I 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01351">
        <property type="entry name" value="NDH1_NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010226">
        <property type="entry name" value="NADH_quinone_OxRdtase_chainI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01971">
        <property type="entry name" value="NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004538">
        <property type="entry name" value="PRK05888.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004539">
        <property type="entry name" value="PRK05888.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849:SF20">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="29"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="68"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="137" mass="15985" checksum="35D2CF151EB3036C" modified="2011-11-16" version="1">MYFRAPYTIYYPFEKGPISPRFRGEHALRRYPSGEERCIACKLCEAICPAQAITIEAEERADGSRRTYKYDIDMTKCIYCGYCQESCPVDAIVETPNVEYSTATREELLYNKEKLLDNGDKWEQEIQYCIDADAPYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ42</accession>
    <name>G3AQ42_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_140713</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376167.1">
        <property type="nucleotide sequence ID" value="XM_007376105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ42"/>
    <dbReference type="STRING" id="619300.G3AQ42"/>
    <dbReference type="GeneID" id="18870313"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140713"/>
    <dbReference type="eggNOG" id="ENOG502RYAS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_1_1_1"/>
    <dbReference type="InParanoid" id="G3AQ42"/>
    <dbReference type="OMA" id="VILIEFK"/>
    <dbReference type="OrthoDB" id="64915at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051450">
        <property type="entry name" value="Gfo/Idh/MocA_Oxidoreductases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377:SF1">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="37"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase C-terminal" evidence="2">
        <location>
            <begin position="178"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="428" mass="47864" checksum="F0C88F6F0BC6F0C8" modified="2011-11-16" version="1">MVSNDIFSNVTSYFENTKNLCNSQTSHNIQAPGSTISFIVIGAGLIGPRHAEHVQARSDCKLMAIVDHSAKGPSVAEKYNCLLFKSLQELFDYCEKFNQTLPDAAIIATPNHTHLQMSLDLASKGIHILVEKPLASNATDCKTLINYCEYKQVKLLVGHHRRFNPYIITTKENMPRVGKLVAIQGTWTICKPPSYFLEKPWRSTVEKGGGTLLINLIHDLDLLQYLIGPIEKVYAELLCKQRTDRFDDNQTPEQELVDEGAALTLKFANGCCGTFVCSDNVTSPFSFEVGTGENPTIPFNNDVAGFYRIFGSHGTISVPDLKLYHQNDSNFESISGNMLTPYPSPDYKIAPIHHMHMGKKPKPFDLQLEHFVNLITGRETEVKCTGEDALRALLCIEAVMKSIETGMPQYVESMDSIQPDYDMLNRYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ43</accession>
    <name>G3AQ43_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_51408</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376168.1">
        <property type="nucleotide sequence ID" value="XM_007376106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ43"/>
    <dbReference type="GeneID" id="18871552"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51408"/>
    <dbReference type="eggNOG" id="KOG4372">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007367_1_0_1"/>
    <dbReference type="InParanoid" id="G3AQ43"/>
    <dbReference type="OMA" id="FSNIGCD"/>
    <dbReference type="OrthoDB" id="5368485at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016445">
        <property type="entry name" value="Rog1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF62">
        <property type="entry name" value="LIPASE ROG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005412">
        <property type="entry name" value="UCP005412_abhydr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="3">
        <location>
            <begin position="210"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="712" mass="81301" checksum="38095A7A35B2654F" modified="2011-11-16" version="1">MLPWYRDRDNLNIGGVNRYTITYTRRDPSQTQVYLRLKNIERSSIRAINLLQGPFILYCHVIPYNYDPHRQFTPDDADTNTEVLFDNQIKPNQSFNATLLLNDNSRVSSSEEGDVFQWEIDIISQIVVSTKSTILFDLMIGDDLALMKRLVRSKISQTLDNLSSPLSPPLEEEIEEFNKEKINHKIYNPQLKVEKLTADDIWDNEPKDPSKPVHLVIVTHGLFSNLTADMLYLKDTLEAKVQENILVRGYRYNAGRTERGVKRLGSNVATYIISLIETTPYKIDKISFIAHSLGGLVQLYAIKYILIHKGADYFEKIHIQPQNLIALASPLLGILNEVSFLISWVLDIGTLGKTGRDLALSKRIPTFGDLYMNEAKRKTFKPILETLPDDPLQIFLSKFKSLTVYANAINDGIVPLRTSALLYLDYEALGDVSELKKTKHIGEHPELEDPENPIETNLTRQSVSEVPEYDIADEEKSAREQMQSKKEAEIWQLAKQPIVAAQELYQQLLRVSSNESKLTKREQKYLKFSAKGTDYNLFENVLPDTSPVDSSGNSLGDEETTVVSSENGLPEKAIVVPPKASAIASAISALICPVPTTNFIINPQSRHHVIFHDKYYHFNDIPKQESNSSTKQSFNDFFRYHGKWKRRKQVIIANKYHAENLNWRKVLVNLPPDAHTNIIVRRRFANGYGWGVIDHLCETLFDRDHNTMKAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ89</accession>
    <name>G3AQ89_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nitrogen regulatory protein areA GATA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_61999</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376214.1">
        <property type="nucleotide sequence ID" value="XM_007376152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ89"/>
    <dbReference type="FunCoup" id="G3AQ89">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ89"/>
    <dbReference type="GeneID" id="18874144"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61999"/>
    <dbReference type="eggNOG" id="ENOG502T5ZK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058653_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQ89"/>
    <dbReference type="OMA" id="CWRRWYK"/>
    <dbReference type="OrthoDB" id="5563539at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007039">
        <property type="term" value="P:protein catabolic process in the vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052292">
        <property type="entry name" value="Glucose_repression_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28051">
        <property type="entry name" value="PROTEIN MTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28051:SF1">
        <property type="entry name" value="PROTEIN MTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="27001" checksum="BD23818C17C5DD6E" modified="2011-11-16" version="1">MYYYDDIVVGPSTQIPTIEQNIDYLAYVPQENTTHYLHDNTNLIKFIKYMNNNREYKVDNMFLYERLSNICWRRLYKNFQKLPEINPFMINWDKNSDVTWLFGPKIESDDEEKQEHGQEQNITLYSCEDNMSVSSFMSDTSVESCYSDEEILRKNSTVSTSSSYYDEEEDKDYNWNLKPILKKTSSESLLDNNVNQKKKVSFNYIVNTREIINGISVDYDFLDHDCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQA5</accession>
    <name>G3AQA5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Multiple RNA-binding domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_140133</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM MRD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376230.1">
        <property type="nucleotide sequence ID" value="XM_007376168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQA5"/>
    <dbReference type="FunCoup" id="G3AQA5">
        <property type="interactions" value="1187"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQA5"/>
    <dbReference type="GeneID" id="18870270"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140133"/>
    <dbReference type="eggNOG" id="KOG0110">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008479_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQA5"/>
    <dbReference type="OMA" id="FNNTCIQ"/>
    <dbReference type="OrthoDB" id="439639at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034462">
        <property type="term" value="P:small-subunit processome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12565">
        <property type="entry name" value="RRM1_MRD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12568">
        <property type="entry name" value="RRM3_MRD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12320">
        <property type="entry name" value="RRM6_RBM19_RRM5_MRD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000247">
        <property type="entry name" value="Multiple RNA-binding domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000459">
        <property type="entry name" value="Multiple RNA-binding domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034482">
        <property type="entry name" value="Mrd1_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050441">
        <property type="entry name" value="RBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48034">
        <property type="entry name" value="TRANSFORMER-2 SEX-DETERMINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="2"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="311"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="494"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="616"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="716"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="152"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="261"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="788"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="178"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="264"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="841" mass="95541" checksum="6E4556D9B51FF294" modified="2011-11-16" version="1">MSRLIVKGLPKYYTEDKLREYFSKQGDVTDVKLMKKRNGESRRFAFIGYKSTEDAEQAARFFNRSFIDTARIDVELAKTFSDPNVPLSFKEKRRRDEDRLRMEEERLIEQENRAQMKRQKVKGVSEIDEEISKNPKLREYMEVMKPSHQVKSWANDSIADGSGGPSVKDLEDALNGKNETKSTVDEHEVLQVEDAGSDDEYTDFNTNRNNEEEEEEEEQMMSLADLNDEQGKEQTQEEENLAANEDVSDLDWLKSRRIRIKENEQEDSNNEQQEQTEEPSEEPKKQPRMQAPVVPQKSPEEQTVEKLQETGRLFIRNILYDSTENDFRNLFEPYGTLEEVHIAIDTRTGKSKGFVYVQFANPEDAVRAYKSLDKQIFQGRLLHILAGDKKKDHRLSEFDLKNLPLKKQRDLKKKAQAAKTQFSWNSLYMNNDAVLESVASKLGLTKAQLIDPQSSSSAVKQALAEAHVIGDVRKYFEDRGIDLTTFDRKERDDKIILVKNFPFGTTIEELGNLFSEYGQIKRMLMPPAGTIAIVEFRDAPSARAAFAKLAYRRFKTSIIYLEKGPKDLFLREPKPEENISVPEIEQSTAVEAKVTAHEILGEEPANNEDVIEGPTVAIFVKNLNFSTTVEALSALFKPLPGFVIATVKTKPDPKKPGNTLSMGFGFVEFKTKEFANAAISNLDGHILDGHKLQLKLSHKQGGSNAAAAAKKSNKSSKLIIKNLPFEATRKDLLELFGAFGQLKSVRVPKKFDSSARGFAFVEFNLMKEAENAMNQLEGVHLLGRRLVMQYAEQDAEDAEAEIEKMTKKVKRQVATQNLAAARLAGKGKIELEEKPDEFESF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQC0</accession>
    <name>G3AQC0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Vacuolar aminopeptidase I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_62038</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the peptidase M18 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376245.1">
        <property type="nucleotide sequence ID" value="XM_007376183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQC0"/>
    <dbReference type="FunCoup" id="G3AQC0">
        <property type="interactions" value="214"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQC0"/>
    <dbReference type="MEROPS" id="M18.001"/>
    <dbReference type="GeneID" id="18874161"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62038"/>
    <dbReference type="eggNOG" id="KOG2596">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019532_3_1_1"/>
    <dbReference type="InParanoid" id="G3AQC0"/>
    <dbReference type="OMA" id="DWPIAKI"/>
    <dbReference type="OrthoDB" id="9880441at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05658">
        <property type="entry name" value="M18_DAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.250.10:FF:000001">
        <property type="entry name" value="Aspartyl aminopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.250.10">
        <property type="entry name" value="Aminopeptidase i, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001948">
        <property type="entry name" value="Peptidase_M18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023358">
        <property type="entry name" value="Peptidase_M18_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570">
        <property type="entry name" value="ASPARTYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570:SF4">
        <property type="entry name" value="VACUOLAR AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02127">
        <property type="entry name" value="Peptidase_M18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00932">
        <property type="entry name" value="AMINO1PTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101821">
        <property type="entry name" value="Aminopeptidase/glucanase lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="518" mass="57558" checksum="31EFC0E975E53ED7" modified="2011-11-16" version="1">MSNSNTFNPEAFLELEQKIRDMMLVAEEAKRRYQETGETETTSAVAKYSKEYYEKKADDYINFTYKNPTIYHVVSYFHEQLNDAGFKYVSEKDSWEDLTPGRYFTTRNGSSLVAFVVGQHWKPKRGVGIIGSHIDALTTVLKPNSTKDNVDGYELLGVAPYAGTLGEIWWDRDLGVGGRLLVKNSSGKIVQKLVDSTPHPIAHIPTLAPHFGTPANGPFNKETQAVPVIGFAGESDEEAEPTSEEKEAPLYGKHPLKLLRYIAGLADVKVSDILQWDLQLYDVQKGVKGGLRKEFVFAPRVDDRVCSFAALHALIEADASDYLKSDAFSLVALVDNEEIGSATRQGIKGGLIEQAVSRILATKFFNPESFDIQEQLRATYANTIILSSDVNHLLNPNFKEVYLEHHKPVPNKGITVALDPNGHMATDSTGLALIEELARKNDDKLQYFQIRNDSRSGGTIGPSISLQTGARTIDLGIPQLSMHSIRAALGTKDIGLGIKFFTGFFKNWRETYDNFVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQC7</accession>
    <name>G3AQC7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF155 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_72276</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMD1/sif2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376252.1">
        <property type="nucleotide sequence ID" value="XM_007376190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQC7"/>
    <dbReference type="FunCoup" id="G3AQC7">
        <property type="interactions" value="77"/>
    </dbReference>
    <dbReference type="GeneID" id="18875423"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72276"/>
    <dbReference type="eggNOG" id="KOG2861">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011220_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQC7"/>
    <dbReference type="OMA" id="QGLYQTK"/>
    <dbReference type="OrthoDB" id="242766at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070131">
        <property type="term" value="P:positive regulation of mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003734">
        <property type="entry name" value="DUF155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051624">
        <property type="entry name" value="RMD1/Sad1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255:SF1">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02582">
        <property type="entry name" value="DUF155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF155" evidence="3">
        <location>
            <begin position="105"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="344" mass="39287" checksum="B5CF6743B8ADD04F" modified="2011-11-16" version="1">MRNSQSKNLKNVPKKKLGAQQLRRADPKFQKTTADILSLLNENRETASFPAASKTLEKHLSIVTSVTVGESINFSQVLSAIQGYDYEVVIPEEVINIEDGPKKLMVLSNGTLVGWNLSEDEIVSTYVPLLGNSIESKYNLFESEELDWIELKTLPENPSYKGNSYLHGEIMVIQGSTIEKRLLDMAAFAVGISRSTRLSVLEDQLEEHLALTKQNSEVLAMGTRITTSEHEFLQLAGRLFLLRGKLNLYSELIDTPDLYWTEPALEKIYESVSKILDINSRIAILNRKLDYATEEQRAFLSVLNERKSARLEWIIIWLIMVEVCFETFHFYERYLDKKESEADK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQJ5</accession>
    <name>G3AQJ5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">STB6-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_154850</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376320.1">
        <property type="nucleotide sequence ID" value="XM_007376258.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQJ5"/>
    <dbReference type="STRING" id="619300.G3AQJ5"/>
    <dbReference type="GeneID" id="18871002"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_154850"/>
    <dbReference type="eggNOG" id="ENOG502QT8Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_323371_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQJ5"/>
    <dbReference type="OMA" id="PKDPFDF"/>
    <dbReference type="OrthoDB" id="19806at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070822">
        <property type="term" value="C:Sin3-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038919">
        <property type="entry name" value="STB2/STB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059025">
        <property type="entry name" value="STB6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31011">
        <property type="entry name" value="PROTEIN STB2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31011:SF2">
        <property type="entry name" value="PROTEIN STB2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25995">
        <property type="entry name" value="STB6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STB6-like N-terminal" evidence="2">
        <location>
            <begin position="58"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="517"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="521"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="965" mass="111215" checksum="00E5F95C06668B6F" modified="2011-11-16" version="1">MSSFAWQVYSPNSIPSYIPKSNGHGANGIVPSISQPSAASSKRGSVATEFSNSNDFITYIIPDFNAVRYFQKEFIKSGEFHLVEESEVTGFEIYLVDQWVNNRNIGTVVTVYTGNESKILVVRFTIIKKPSKYYPPRFQEYLNELIQNHSRMKKMENERRPRDASNEVCFVTNLTSLPSNLNLIPIPHGDVRKVESAFIINSNLRKLHCTGRSISLISEKISDASEGKFRQMYKIYNVPVKFASRELVNVVQTCLFYFDLLDARYCNGLLCAKTEEAINNWWNLVGLPHFNIKPNRKDGILPAMTVAAIISLILSVRLRIQIVGGSDVPKDPFDFENFMIAIGQFQRQYKLDKTRKLDMETLNRLFNITNARLLPEKHANYFYSSAYGDNSIYDSDYTYEFPSSPPQRKENLPKRYGKELKKLTNVVKTVQGHIASKADLDEIQTTPQNSTSRPTTGRIMNKIAKLADMSSPLDVETLDLEYLVKNYLTGKVLIRLFYGVQNGATADKLIEQTGLKEADHHHHHHHHMRRRGRSITDNTNTNSYVFESLRDKIANTQELALGDYRYSRGFNFKMFGLQSKRNLVPERKRTVVERTLEPSSRVDEPSTSLVDTFLQIGGGDTSSYAESIGSGKNNVVNGGGGGTGSLVYNSTTELNHPIVKFQRNLNRRNSFPFMLENEANLNSLVVSKVDTRINSEMVEIERKRPRSFSYSCVETVAPDPQISTVAKLSQRYLKNINKLMKYENLRKLYFEEKDKKEYVTNKSLEHSYQLMNLELVKLNNLRVQMDSHRAKIMDEGFADHLQYNMNLLTTTIDRLVYETRIVVKRIDELEDNYQFFEMKNDDTQKKMTKVIDNLIRSTKFKLVYPDPQERKRIIVKLVGEDDERYIQQGTVDYSRGGGLKIYFMMIFEFVWTYFQIFRANMNADRIRAAWGKLDPNRTIINKAYSYMHRQPVQASQTTEVREELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQT6</accession>
    <name>G3AQT6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_155078</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376411.1">
        <property type="nucleotide sequence ID" value="XM_007376349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQT6"/>
    <dbReference type="GeneID" id="18871012"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_155078"/>
    <dbReference type="eggNOG" id="ENOG502RPWB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068906_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQT6"/>
    <dbReference type="OMA" id="QWVNITT"/>
    <dbReference type="OrthoDB" id="4018855at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442567" evidence="1">
        <location>
            <begin position="21"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="292" mass="31686" checksum="5C077E026485119D" modified="2011-11-16" version="1">MQLTKFSALLVLAYCTVSSGSPVEINAISATNTAEIKALVSSFEQFNSEFALVKRDGQLDVPLNQLVTRADVPILQSILSGLNNSGLALIVIDYVLLRPELLDITIDATIWVIESGLINLTDLLIALEQSGLIIDVLMLSLSDPDILPGLLRIGRELLKQSGIDIFSKRDAFAEAVSTESPEQAFAELSKRESEVMNSLFTALRESGLALSVVQHLLTTPELAAPNAHFLLSILRSNALSLTSLLNALKQSNLIWNLLRDILGNPDVLSQFGQIIADRIAKGIIPKNLFDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR91</accession>
    <name>G3AR91_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_67342</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376044.1">
        <property type="nucleotide sequence ID" value="XM_007375982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR91"/>
    <dbReference type="STRING" id="619300.G3AR91"/>
    <dbReference type="GeneID" id="18875156"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67342"/>
    <dbReference type="eggNOG" id="KOG1001">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003233_0_0_1"/>
    <dbReference type="InParanoid" id="G3AR91"/>
    <dbReference type="OMA" id="LLHEMNY"/>
    <dbReference type="OrthoDB" id="2801544at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF51">
        <property type="entry name" value="SNF2-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="945"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1079"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1098"/>
            <end position="1108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW31266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1121" mass="129178" checksum="C8BBEB29D09DA01A" modified="2011-11-16" version="1">MAHERSTFQKAIEITNELRKLIPIGTLNFYDLPLNDSDLGGLSVEQDPDSLWKWFDIDFFIYICKGKVCQVLSYLQFLIDFQFMKATFRIVSFKEDSSVKICKVRLYGVPLDIQGGRYIQEWRASTPKSYNIDKNYQRYLDSILPYIDFSNRGWLLDDGKVIPLEHLLPLVLLRCKETIKQEDLSIEEFNFHVERWTNNSTVVPYKGPSSNSSSFSKRIRDIYNEVPSPVVNHNIQVHTSITGVTSTLYPFQARSVAKMLEKETIPGRALVPNLISVKCPDGSQEYYFDLKTKCIFESPELLSLPRGGILAENMGLGKTLISLSLISASKYEVSKIPSDLLIHNSEESEIPEYEFKGNFSKPVKEKMNVKSLVEICSDIIGQDSIPWKHHINNLPQSVIATLTNNPGYFRIPLDNDEFVSPYSQRKRHSLRRVERGVFNFRKLYLCSTTLVIVPDNLFIQWNEETNKHLEPGYLKILFISNYYKMKQPDSCNTYSDFIPLEPTILIQYDLILLSRTIFARSVDNDSNPLASSFLARSLFAERKWAVTGTPTSGLTRLYMDELEQHQARKSKYVVKNSFNEKADLQKLGIIIGNFLKLEPFASQPKLWNSQIIQPLLANYYGSELLFSNLLHSIMVRHNPRDVEQDLELPPLHHDAVFLEPSYHNIMSINLFTAVLAVNAVTSERTDVDYMFHPSNRSQLRKLITNLQRATFYWTGFKQEDVETLIKICLSSLKKKTQSGISKYSGRDTELLEKSIAVAQLALRNLQWRTSSLLHEMNYYVSNLPDIFMRTYGIGVANDKVGVYGAPHLTSIQEFFFKNRFIDYTNVERVTELLEEKSKEFWKKYWQENVKRNAERFNKQDPNQDVESRIKEGAIENSMDIPDIALSTPKKSRPKKEFSSPKEELEREVGHLNYHNHNETVSYDKIRRAKVLGTASAKLSYLVSRLLDHQETKIKSLVFFEFEDSAYYLTEALDILGINYILYATSITPAQRAKNLVEFANHDVEVDGGLSLIMDLRLAAHGLTILSATRVYFISPVWSQSIEAQAIKRAHRIGQTKDVYVETLILKGTLEEEIYKRRTNDVEQEQLGPNGEETNEEEQNGRDKRRFVIDDTGMQDTRSELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARQ0</accession>
    <name>G3ARQ0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62396</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376581.1">
        <property type="nucleotide sequence ID" value="XM_007376519.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARQ0"/>
    <dbReference type="GeneID" id="18874308"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62396"/>
    <dbReference type="HOGENOM" id="CLU_1090578_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARQ0"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="30323" checksum="4496CCA5C7B22F1C" modified="2011-11-16" version="1">MLQQQLARLDVINQLQLYNDLRFSYSMEDNYSIERLYIEGVERLYSDPLAIIPHLKALTTLCIFDVTTINILKVFRRRNITNLKLKRLSLQVCKIPIRELGEIFDLREIVYFELVFFKNVRPYSDLKWLASNMPKLRDIKIVWWNASFEKIMEQLAGHRIVNVRLHSLQPKKEGDIQRCESTMPNLIKGHERSLTHFSLYSGMEEITGIWDRHKWYTRKDPSDNSKVNTHIKIFSRRKFFLKMDAYGQQQIVIAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3ART9</accession>
    <name>G3ART9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF155 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_67423</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMD1/sif2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376134.1">
        <property type="nucleotide sequence ID" value="XM_007376072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ART9"/>
    <dbReference type="FunCoup" id="G3ART9">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ART9"/>
    <dbReference type="GeneID" id="18875161"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67423"/>
    <dbReference type="eggNOG" id="KOG2861">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011220_1_2_1"/>
    <dbReference type="InParanoid" id="G3ART9"/>
    <dbReference type="OMA" id="SHRHGEY"/>
    <dbReference type="OrthoDB" id="18302at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003734">
        <property type="entry name" value="DUF155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051624">
        <property type="entry name" value="RMD1/Sad1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255:SF15">
        <property type="entry name" value="SPORULATION PROTEIN RMD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02582">
        <property type="entry name" value="DUF155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF155" evidence="3">
        <location>
            <begin position="204"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="488"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="488"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="512" mass="58655" checksum="07EA376B9B69EF2D" modified="2011-11-16" version="1">MSQDSDTTPLLTTDSLNLSHENPLMATTATNGIQKPPAKRPTKNTPPTSKSTGKITNSGTIGLQRTSRTSQKLKLLPEDPTLDHDNNNNDDIDDPQQARVYSQVKKITDTSARKDAEILGKSHRDLLPRVTAYCTCGSYKMKDLLRWLKDRKRIHNTSPKLFDECLYTPFTYKDWRGEEEEAGHKLIRLADEGGEIDIGKKNDIFVFEYGVVIMWGYTTKEEAAFLEDLAKFETEKLSQEDIQIEEFNYYITKSYQPRIYNDFITLRDDDNYMLKLSISHALAQSVKISLFEELVDNTIEDTQDIPQQIARTGKVEMTRDEIMKSIGELFILRININLHGSVLDSPELMWAEPHLEPIYQATRGYLEINQRVDLLNQRLEVISDLLQMLKEQLGHSHEENLEYIVVVLNNLDYTFIPDLSNTEVLGQDLIIRGKLFTVHPGPSRVRFIAQTRSRAVWKRSDFQHGFPTKCDGIYHVINTRDPFYINSSDLNTDHSSSQESIPDVPEKGGIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARX4</accession>
    <name>G3ARX4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutamate-5-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="2">1.2.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Glutamate-5-semialdehyde dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Glutamyl-gamma-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_62440</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19541"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the gamma-glutamyl phosphate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.41" evidence="2"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376601.1">
        <property type="nucleotide sequence ID" value="XM_007376539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARX4"/>
    <dbReference type="FunCoup" id="G3ARX4">
        <property type="interactions" value="910"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARX4"/>
    <dbReference type="GeneID" id="18874317"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62440"/>
    <dbReference type="eggNOG" id="KOG4165">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030231_0_1_1"/>
    <dbReference type="InParanoid" id="G3ARX4"/>
    <dbReference type="OMA" id="KTQRYGT"/>
    <dbReference type="OrthoDB" id="1934954at2759"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004350">
        <property type="term" value="F:glutamate-5-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07079">
        <property type="entry name" value="ALDH_F18-19_ProA-GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000006">
        <property type="entry name" value="Gamma-glutamyl phosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00412">
        <property type="entry name" value="ProA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012134">
        <property type="entry name" value="Glu-5-SA_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000965">
        <property type="entry name" value="GPR_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001221">
        <property type="entry name" value="PRK00197.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00407">
        <property type="entry name" value="proA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063:SF8">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063">
        <property type="entry name" value="GLUTAMATE SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000151">
        <property type="entry name" value="GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="12">
        <location>
            <begin position="3"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="437" mass="47759" checksum="41718A80989D57C0" modified="2011-11-16" version="1">MSAESIAKAANLAFSSLKTLSNTQRSTALQKIHDALAAHKDQVLEANKLDMDAAIEHKFSSSLIKRLDLSSPGKYDGMLQGILDVANLEDPVGKITLAKKIDEGLNLYRVTAPIGVLLIIFESRPEVIANITALAIKSGNSAILKGGKESYQTFKIMSDIVNETLAKDTDVPKDAIQLIQSREDVADLLSQDKYIDLVIPRGSNALVRNIKSNTKIPVLGHADGICSIYVDENFDIVKAKRIVVDAKTNYPAGCNAVEQLLFNKNIPVEQINEILQTLIAAKVTIHVTEQVKQILTIGESEYIKDAEEDAFDKEWLSLDISVDLIDNVSAAIAHINLHSSKHTDCIITEDKAKANLFLHGVDSAGIYWNASTRFADGFRYGFGTEVGISTNKIHARGPVGLEGLMSYQYHLKGDGHIASEYVGAGGRKHFIHEDIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARZ7</accession>
    <name>G3ARZ7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.205</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2'-O-ribose RNA methyltransferase TRM7 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_140790</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>cytidine(32)/guanosine(34) in tRNA + 2 S-adenosyl-L-methionine = 2'-O-methylcytidine(32)/2'-O-methylguanosine(34) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42396"/>
            <dbReference type="Rhea" id="RHEA-COMP:10246"/>
            <dbReference type="Rhea" id="RHEA-COMP:10247"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74445"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.205"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. TRM7 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.205" evidence="7"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376624.1">
        <property type="nucleotide sequence ID" value="XM_007376562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARZ7"/>
    <dbReference type="FunCoup" id="G3ARZ7">
        <property type="interactions" value="933"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARZ7"/>
    <dbReference type="GeneID" id="18870318"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140790"/>
    <dbReference type="eggNOG" id="KOG1099">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009422_1_2_1"/>
    <dbReference type="InParanoid" id="G3ARZ7"/>
    <dbReference type="OMA" id="FIVCLNF"/>
    <dbReference type="OrthoDB" id="289250at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106339">
        <property type="term" value="F:tRNA (cytidine(32)-2'-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106340">
        <property type="term" value="F:tRNA (guanosine(34)-2'-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106050">
        <property type="term" value="F:tRNA 2'-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002130">
        <property type="term" value="P:wobble position ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000176">
        <property type="entry name" value="tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03162">
        <property type="entry name" value="RNA_methyltr_E_TRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028590">
        <property type="entry name" value="RNA_methyltr_E_TRM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF12">
        <property type="entry name" value="TRNA (CYTIDINE(32)_GUANOSINE(34)-2'-O)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="8">
        <location>
            <begin position="21"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="295" mass="33003" checksum="C9ABBFD36B1BBB23" modified="2011-11-16" version="1">MGKSSKDKRDLYYRRAKEEGWRARSAFKLLQLNQEFNLFDGITRVVDLCAAPGSWSQVLSRELQGKNAKIVAVDLQPMTPIDGVTTIQADITHPNTLQTILDIFAGEQADFVCSDGAPDVTGLHDLDEYIQAQLILSALQLTTCLLKPGGTFVAKIFRGRDIDLLYSQLSYLFERVICAKPRASRGTSLEAFIVCLGYKPRPGWNPKLDLQTSTEEFFEGADIGKAGNLEHFELPDDEERKIAKFVACGDINDVDSDATYTLELNRLALEPVQMPTAPPYKKALEMKRRGDLIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASH6</accession>
    <name>G3ASH6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Signal recognition particle receptor subunit alpha homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Docking protein alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_56999</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endomembrane system</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP-binding SRP family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377127.1">
        <property type="nucleotide sequence ID" value="XM_007377065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASH6"/>
    <dbReference type="FunCoup" id="G3ASH6">
        <property type="interactions" value="790"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASH6"/>
    <dbReference type="GeneID" id="18872089"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56999"/>
    <dbReference type="eggNOG" id="KOG0781">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_8_1_1"/>
    <dbReference type="InParanoid" id="G3ASH6"/>
    <dbReference type="OMA" id="TDLLYDI"/>
    <dbReference type="OrthoDB" id="1727884at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005785">
        <property type="term" value="C:signal recognition particle receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000566">
        <property type="entry name" value="Signal recognition particle receptor subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007222">
        <property type="entry name" value="Sig_recog_particle_rcpt_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF1">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04086">
        <property type="entry name" value="SRP-alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="13">
        <location>
            <begin position="521"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="130"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00081194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW31094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="548" mass="60554" checksum="2CD924907FFC7E36" modified="2011-11-16" version="1">MSQSLIIFRPSGKVEYQFNTKIHPFNDLISHIVNETCIDLAPNFVTKTISQKRFYSLHRKGGDDVGSSGDLYAALYFDAVTTIDSEKIRSTLNGILKTFVKLSEEGDDEDHSKLKKLVDLQFNQLMKLKQSEESATPVRERTPLSGHASGTSTPSAKKAKGGKKMRKWDGEEMIDFDGKEALDYSNGQVEGEVGEGDKSILNIDQTAFVKDNDLVLVNELNDILNESDDEEQEEGAGTQKSGFFSKISSYFGGAINVDEVSKKFHDQLISKNIAPQTSKSIIEKIKLKLQNKSNITMAVYQQTLTDELTKILTPNVSTDLLYDIKKQKTRGTRPYVISVVGVNGVGKSTNLAKLAYWFLQNNLNVLICACDTFRSGAVEQLKVHVNNLQKLNDMSTTTSSKIELFEKGYGAGDHVVQTAKSAIQFATDEKFDIVLIDTAGRTHSNAKLMAPLKKFGDAADPDKIIMVGEALVGTDSVEQAINFNNAFGNKRNLDFFIISKVDTVGDLVGTMINMVMATKVPILFVGTGQTYTDIKRLSVKQVVEMLMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASN0</accession>
    <name>G3ASN0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_142779</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376750.1">
        <property type="nucleotide sequence ID" value="XM_007376688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASN0"/>
    <dbReference type="FunCoup" id="G3ASN0">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASN0"/>
    <dbReference type="GeneID" id="18870484"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142779"/>
    <dbReference type="eggNOG" id="ENOG502S59H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133944_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASN0"/>
    <dbReference type="OMA" id="HRANIHI"/>
    <dbReference type="OrthoDB" id="4082971at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000171">
        <property type="term" value="F:ribonuclease MRP activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034965">
        <property type="term" value="P:intronic box C/D snoRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000294">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, RNase MRP-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014612">
        <property type="entry name" value="Pop7/Rpp20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020241">
        <property type="entry name" value="RNase_P/MRP_Pop7_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28256">
        <property type="entry name" value="RIBONUCLEASES P/MRP PROTEIN SUBUNIT POP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28256:SF1">
        <property type="entry name" value="RIBONUCLEASES P_MRP PROTEIN SUBUNIT POP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12328">
        <property type="entry name" value="Rpp20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="132" mass="15359" checksum="79451E5F4AE0C3BC" modified="2011-11-16" version="1">MTKRIQGKHIKHTANIKPRTQTEQISTFLIKSQTPYVSAIKKISKQLSKFEHSTGVKKYSQYKQVSYITVKGMGKSIEKTLSVGLYFQERYNVDIHTGTVEVLDEFQQDEDESVYKKRLVSSVELKIWLKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASP0</accession>
    <name>G3ASP0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing]</fullName>
            <ecNumber evidence="3">1.3.1.124</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_141696</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a (2E,4E)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:45912"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85101"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a (2E,4Z)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:61892"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85099"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="1.3.1.124" evidence="3"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376759.1">
        <property type="nucleotide sequence ID" value="XM_007376697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASP0"/>
    <dbReference type="FunCoup" id="G3ASP0">
        <property type="interactions" value="260"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASP0"/>
    <dbReference type="GeneID" id="18870392"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141696"/>
    <dbReference type="eggNOG" id="KOG0725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_1"/>
    <dbReference type="InParanoid" id="G3ASP0"/>
    <dbReference type="OMA" id="MQAHVCA"/>
    <dbReference type="OrthoDB" id="2136131at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008670">
        <property type="term" value="F:2,4-dienoyl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05369">
        <property type="entry name" value="TER_DECR_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045017">
        <property type="entry name" value="DECR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43296">
        <property type="entry name" value="PEROXISOMAL 2,4-DIENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43296:SF2">
        <property type="entry name" value="PEROXISOMAL 2,4-DIENOYL-COA REDUCTASE [(3E)-ENOYL-COA-PRODUCING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00026117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="290" mass="30678" checksum="1A16671214B9E186" modified="2011-11-16" version="1">MGNTLDQSYVTRSAWKPDIFKGKVVFVTGGAGSICRVQTEAMVLLGANATIIGRNVAKTETTAKEIESLRPGAKVIGLGNVDVRDVNSLKKAVDHTVEQLGKIDYVIAGAAGNFLCDFNHLSSNAFKSVISIDLLGSFNTVKACFEQLRKNKGAIIFVSATLHYYGVPFQSHVGAAKAGIDALSNALAVEFGPLGIRSNCIAPGAIDGTEGMARLAPPSATPFVNKIPLQRMGTTEDIADNTVYLFSPAASYITGTISVVDGAWWHIGGVMGDLYPSIVEQANNDPETKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASX5</accession>
    <name>G3ASX5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_72688</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376790.1">
        <property type="nucleotide sequence ID" value="XM_007376728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASX5"/>
    <dbReference type="STRING" id="619300.G3ASX5"/>
    <dbReference type="GeneID" id="18875449"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72688"/>
    <dbReference type="eggNOG" id="KOG0029">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004498_10_1_1"/>
    <dbReference type="InParanoid" id="G3ASX5"/>
    <dbReference type="OMA" id="DELMIRC"/>
    <dbReference type="OrthoDB" id="5046242at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF410">
        <property type="entry name" value="LYSINE-SPECIFIC HISTONE DEMETHYLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="1">
        <location>
            <begin position="13"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="477" mass="54664" checksum="2FAD68F0BFD1B128" modified="2011-11-16" version="1">MIHKKVVIIGGGISGVKAAIDLYQAGITDTIIVESRDRLGGRLWTINSEKTRGLKFDMGASWFHDTLNNPLFERAIKSKIVKYHFDDGKCQYVSQDDGDVPSWKFQTIVDEMMSYFKLAYDKDPSKPDVSLKRLCEEYMHKYKDRLTEEQKQYALGVIRALAELYHGETWDTLSGKYCFANMNQHVGRRAFVENGYYNVFQTVLKELPREYLNHNVKLNAHVDKIDYSNPSKVIVHLFDGRSYSCDYLICTIPQSILQITDPKDSCYLKWEPTLPPPLQKVLPDIHFGSLGKLVFEFNECFWPEDVERFYAITNTTSKPDLLGDLSFNAWDFPSVIVNYQALMRLPSLVILTQNPLSKYIERLAKEDKQQDIWKLFKPVLEQASLVPKGHQIPDPVAIYNSPWNGEYLTRGSYGTTPVGMNDPYKINQILNQGFQNRIRFAGAETMNGSANGCAHGGWFSGEREARFIIESEKKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASZ4</accession>
    <name>G3ASZ4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Carbamoyl phosphate synthase arginine-specific small chain</fullName>
            <ecNumber evidence="3">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Arginine-specific carbamoyl phosphate synthetase, glutamine chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Glutamine-dependent carbamoyl phosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_62632</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer composed of 2 chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CarA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="3"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376809.1">
        <property type="nucleotide sequence ID" value="XM_007376747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASZ4"/>
    <dbReference type="FunCoup" id="G3ASZ4">
        <property type="interactions" value="371"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASZ4"/>
    <dbReference type="GeneID" id="18874412"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62632"/>
    <dbReference type="eggNOG" id="KOG0370">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035901_2_1_1"/>
    <dbReference type="InParanoid" id="G3ASZ4"/>
    <dbReference type="OMA" id="CFSVQYH"/>
    <dbReference type="OrthoDB" id="434at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000016">
        <property type="entry name" value="Carbamoyl-phosphate synthase arginine-specific small chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.20:FF:000003">
        <property type="entry name" value="Carbamoyl-phosphate synthase arginine-specific small chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="entry name" value="Carbamoyl-phosphate synthase small subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009475">
        <property type="entry name" value="PRK12838.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF7">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="Carbamoyl phosphate synthetase, small subunit N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbamoyl-phosphate synthase small subunit N-terminal" evidence="16">
        <location>
            <begin position="34"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW30776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="429" mass="47206" checksum="2E39AA50D5593C8D" modified="2011-11-16" version="1">MITAFRQIASKRCLATAASPAAKLPFTDNKSKLDRATLTIKNGPVFSGYSFGANKNVSGEAVFTTSLVGYPESMTDPSYKGQILCFTQPLIGNYGVPSSSVKDQFDLLKYVESPQVQCIGIVVADVALEYSHWTAVESLQQWCQRSGVAAITGVDTRQLVTYLREQGSSLGKITIGEEYDADEDAAFDDPETVNLVHKVTTKAPFHIACPPEHSKNIHIAVLDCGVKENILRCLVQRGASLTVFPYDYPIHKIANKFDGVFISNGPGDPTHCSSTVQHLRQTMDTHRELPIFGICLGHQLLALASGAKTIKLKYGNRAHNIPALDLITGKCHITSQNHGYAVDATTLSPEWEPYFTNLNDLSNEGMIHKFQPVFSTQFHPEAKGGPLDTSFLFDKFFENIETYRSNNGLNLANVDNSLLVDILPKQRVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT61</accession>
    <name>G3AT61_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">2-deoxyglucose-6-phosphate phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_142765</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376858.1">
        <property type="nucleotide sequence ID" value="XM_007376796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT61"/>
    <dbReference type="FunCoup" id="G3AT61">
        <property type="interactions" value="207"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AT61"/>
    <dbReference type="GeneID" id="18870482"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142765"/>
    <dbReference type="eggNOG" id="KOG2914">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_13_4_1"/>
    <dbReference type="InParanoid" id="G3AT61"/>
    <dbReference type="OMA" id="NYEHRVR"/>
    <dbReference type="OrthoDB" id="18978at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003850">
        <property type="term" value="F:2-deoxyglucose-6-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051806">
        <property type="entry name" value="HAD-like_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43481:SF9">
        <property type="entry name" value="2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43481">
        <property type="entry name" value="FRUCTOSE-1-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01228">
        <property type="entry name" value="COF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="253" mass="27963" checksum="A6297920535C7C2D" modified="2011-11-16" version="1">MSNIITLNTNFILFDLDGTLVNSTAAVEKSWEHEVEVHNKQHPDAKLDLQTLLFECHGSRTVETFTKSFPEKLPVSQKDIDIWESEIVRKYGDLGKPIPGSVAFLESLNEKGAKDSWAIITSGTQELAYGWYNRLFSSIPRPKIFITAHDVSNGKPDPEGYYTAFKKLISLNNIVVGVSRPKAIVFEDAPTGVKSGVNGGFLVIGIASTFSKEVLLKAGATYVIEDFSKVSLIESDDADEDEYSRLTVSLEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT70</accession>
    <name>G3AT70_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62700</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376866.1">
        <property type="nucleotide sequence ID" value="XM_007376804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT70"/>
    <dbReference type="FunCoup" id="G3AT70">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AT70"/>
    <dbReference type="GeneID" id="18874437"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62700"/>
    <dbReference type="eggNOG" id="ENOG502RZPK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_665828_0_0_1"/>
    <dbReference type="InParanoid" id="G3AT70"/>
    <dbReference type="OMA" id="MKVIIVR"/>
    <dbReference type="OrthoDB" id="1932312at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="413" mass="47233" checksum="C64EE742FFFCF7A8" modified="2011-11-16" version="1" fragment="single">MSKVTTPVHNLIAHFLAENGYEKTLREFQSEHGQPIDQVKVGDESLTDIISDRINFNQLAPSLENLDIGNRRLSFSGSGYLKSVSKDQLQNWVTPFPHLAKELVDIDGMVISTSYLDGCVYFSTNNSQIYIVDDKHVVYRSKFPVVIKKIIPIKQDQLLLVGMSGMFYLYFKQNERWQTVVEFPVHKRLIIDAKHVRFNDCDYIVTLGWDFYLKVIKLEAENKFTIVSEFKLNQQGTCFDLINHNNQLLIVVGKLDNTLLDVFELQENKLELLFKISLNDAEFSASEFSPRYITIQHFGSSVPLIAVATSHEPYMRLIVVTLDMIEGQEHEIKRNQIVKNLNTMSPQDKFSQPIIAWRLPKHVNGADVTIEATKSSGIWIMGDDGVIRGIDLVDDKVVVELKDVHQGKIKDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT89</accession>
    <name>G3AT89_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAC3/GANP/THP3 conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_142098</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376885.1">
        <property type="nucleotide sequence ID" value="XM_007376823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT89"/>
    <dbReference type="FunCoup" id="G3AT89">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AT89"/>
    <dbReference type="GeneID" id="18870421"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142098"/>
    <dbReference type="eggNOG" id="KOG1861">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015513_4_1_1"/>
    <dbReference type="InParanoid" id="G3AT89"/>
    <dbReference type="OMA" id="RETMNFK"/>
    <dbReference type="OrthoDB" id="199574at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045107">
        <property type="entry name" value="SAC3/GANP/THP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005062">
        <property type="entry name" value="SAC3/GANP/THP3_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436">
        <property type="entry name" value="80 KDA MCM3-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436:SF4">
        <property type="entry name" value="LEUKOCYTE RECEPTOR CLUSTER MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03399">
        <property type="entry name" value="SAC3_GANP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC3/GANP/THP3 conserved" evidence="2">
        <location>
            <begin position="183"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="461" mass="53719" checksum="34421D844576F255" modified="2011-11-16" version="1">MHTGNSYNSTGNSNSDTPSSWDEAPTNTNDNTNQDWPPALSQYVTVCFNRSSTLSERDKVLFNRQLQSLIETAVDDNLIWTNDWSKQKLPILDGEHITLELEKQKKKSKTSSKPIKKESNDSLSKSPSVNTLGDFDSNERKRQRMERFNDNNSSTPSVSTPISKAGSVIGRCTDLEKSYLRLTSEPDPNRVRNQQVLQKSLKFVQEKYLQTKNYSYALDQFRSIRQDLMVQNIKNDFTIVVYETNAKISLENDDLGQFNQCQSQLKYLYHLTRKNNSAFTKRFFRLEVEFLMYQVVYMMITFNVSEIFRIKMGILQQFSDFRTTDREMTLFKFIQTLFQCQVYITTGNYLRFFDSLNQFEQEQDIKLALAVLKKHLYQKFRVKSMSIITQSFRNYPVKVLTDCLGFSNYTECFEFLESIGLKDYVTASDDNGQLECHKCKHLVVAMMQKSNFKKVDIKGQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATK8</accession>
    <name>G3ATK8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome biogenesis protein YTM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">YTM1</name>
        <name evidence="9" type="ORF">SPAPADRAFT_62873</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat WDR12/YTM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377004.1">
        <property type="nucleotide sequence ID" value="XM_007376942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATK8"/>
    <dbReference type="FunCoup" id="G3ATK8">
        <property type="interactions" value="943"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATK8"/>
    <dbReference type="GeneID" id="18874502"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62873"/>
    <dbReference type="eggNOG" id="KOG0313">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_0_1"/>
    <dbReference type="InParanoid" id="G3ATK8"/>
    <dbReference type="OMA" id="DHKYVEF"/>
    <dbReference type="OrthoDB" id="10251381at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110136">
        <property type="term" value="P:protein-RNA complex remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000706">
        <property type="entry name" value="Ribosome biogenesis protein YTM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03029">
        <property type="entry name" value="WDR12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012972">
        <property type="entry name" value="NLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028599">
        <property type="entry name" value="WDR12/Ytm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855:SF11">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN WDR12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855">
        <property type="entry name" value="WD40 REPEAT PROTEIN 12, 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08154">
        <property type="entry name" value="NLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NLE" evidence="8">
        <location>
            <begin position="8"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="155"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="209"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="286"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="372"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW30971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="455" mass="50788" checksum="6258E5B93D2C01D2" modified="2011-11-16" version="1">MPEQTNQIKIKFFTNDEDESLHVSDSPLFVPVSLKRFGLSEVVNHLLEKDNDTPIPFDFLIDGVLLRGSLEEYLTKHGLSNEAFLSLEYQRAILPPSFLASFNNDDWISSLDAINLQSQAVTSSNLSITQAKILSGSYDGIVRTYNMSGKVEKQYVGHNAPIRAVKWISPTRIVSAGNDRQVRLWKTSHEEIEDEDEDEPEEGKTLAILEGHKAAVVDLSVEYSTNRILTASYDQSIGFWSTNYKEMQSIQPFEYDSNVISSSSKKRRKMALQDASIRRRSPLSLLEGHTQPVEAVIFDANDATVGYSVSQDHTIRTWDLVTSRCVDTRTTGYSLLSLLQLPKQNLLVTGSSARHINLHDPRTTTEQTSTKLVGHTNFVVALSASPNNEHMFASASHDGTVKVWDVRSEKSLYTITRQDGSKGKIFDVCWDGQIGIISGGEDKKIQINKGSDISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATL0</accession>
    <name>G3ATL0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HPt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_56906</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377006.1">
        <property type="nucleotide sequence ID" value="XM_007376944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATL0"/>
    <dbReference type="STRING" id="619300.G3ATL0"/>
    <dbReference type="GeneID" id="18872079"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56906"/>
    <dbReference type="eggNOG" id="KOG4747">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085158_2_0_1"/>
    <dbReference type="InParanoid" id="G3ATL0"/>
    <dbReference type="OMA" id="LVEWSVF"/>
    <dbReference type="OrthoDB" id="1673781at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043424">
        <property type="term" value="F:protein histidine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00088">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="entry name" value="HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045871">
        <property type="entry name" value="AHP1-5/YPD1"/>
    </dbReference>
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        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28242">
        <property type="entry name" value="PHOSPHORELAY INTERMEDIATE PROTEIN YPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28242:SF52">
        <property type="entry name" value="PHOSPHORELAY INTERMEDIATE PROTEIN YPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01627">
        <property type="entry name" value="Hpt"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histidine-containing phosphotransfer domain, HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HPt" evidence="3">
        <location>
            <begin position="30"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="110"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="110"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine" evidence="1">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="173" mass="19387" checksum="1BB2A0EEC4B636E4" modified="2011-11-16" version="1">MSEDKKKKLQATGLVDWPVFSEIIAMDEDEEEFSKSLIQTFYTQVEETFQKFSNLINEKNLEELSKAGHFLKGSAAALGLGTIATQCERIQNYGHKINFDNFEYPATATTTTTTSSPHSIHTSSSENNTNNNNTTTTPDESSDDFWIALIKDALEKAQDGYTKSKKALTDYYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATL9</accession>
    <name>G3ATL9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_52171</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007376953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATL9"/>
    <dbReference type="GeneID" id="18871636"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52171"/>
    <dbReference type="eggNOG" id="ENOG502T63D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1644763_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATL9"/>
    <dbReference type="OMA" id="QESDEIM"/>
    <dbReference type="OrthoDB" id="4018315at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031511">
        <property type="term" value="C:Mis6-Sim4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018565">
        <property type="entry name" value="Nkp2/Cnl2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28064">
        <property type="entry name" value="INNER KINETOCHORE SUBUNIT NKP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28064:SF1">
        <property type="entry name" value="INNER KINETOCHORE SUBUNIT NKP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09447">
        <property type="entry name" value="Cnl2_NKP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="161" mass="18691" checksum="80F4BAE1D4DF1194" modified="2011-11-16" version="1">MESSSILSNYLMSSTEIKSDISFESFSREFPSRTPQDVVRAIYDQLIIQQEQNIRSKVIDTINNTYKFPVNQVSSVIQKNSSVKEKKLNNLINQMEKLASTLSLQEQSLSEESDQYIKEIKLLIGELSSLRYGRKDDFYEPAMDEARECIAKIKKFFDIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATQ7</accession>
    <name>G3ATQ7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lethal giant larvae (Lgl)-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_52383</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat L(2)GL family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377254.1">
        <property type="nucleotide sequence ID" value="XM_007377192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATQ7"/>
    <dbReference type="FunCoup" id="G3ATQ7">
        <property type="interactions" value="151"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATQ7"/>
    <dbReference type="GeneID" id="18871672"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52383"/>
    <dbReference type="eggNOG" id="KOG1983">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006030_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATQ7"/>
    <dbReference type="OMA" id="QIYVFGQ"/>
    <dbReference type="OrthoDB" id="19944at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045159">
        <property type="term" value="F:myosin II binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013905">
        <property type="entry name" value="Lgl_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH_b-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241">
        <property type="entry name" value="LETHAL 2 GIANT LARVAE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241:SF25">
        <property type="entry name" value="TOMOSYN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08596">
        <property type="entry name" value="Lgl_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="Soluble quinoprotein glucose dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="258"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Lethal giant larvae (Lgl)-like C-terminal" evidence="7">
        <location>
            <begin position="560"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="989"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="997"/>
            <end position="1010"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1080" mass="118103" checksum="4E33AA1AB89677C2" modified="2011-11-16" version="1">MFSKLKSRKAPLSLSSVSNVIKTSGAKDLSPKEIKGKNIDLKVINQLGIPRSSVVAVAYDPVQSLLAISTTNNDVRVYGQVNVEVVFEFNLKHPITFLRFIKGVYLVCWSSGSGLSILSLHSKKILGTYSFSGAITCAEADPSLDFLIFGLNNGSVLFYDVDRMNLTPFRIDNLQKKVLPKEKMSHVVAIEWHPRDIGTLLIAYDKCAIIYSLMSGEIKNTFTYQLQKGARAFEYSSYILHGGKKKLFSSSKDITPDLTSAHFHPNGLHIVTTHIDNSIVFWDISSPTPLEARTVFETHIHKPGNPIPIPADGFNLIEDVKWVCGEDPENTKLLICGGDAHSTNVVHVLDFGYTLKYSLTSYDKQSEFYASPQLGQRMIPITFYLNETNEKEKLVSIQPMNDNGLPYFHGGHNPAYLLLVSNLGQIYFVLFSDNAGGQGSTDLGRVVLPTSVSFVHPPLTSYNLEEVFRMDWYSIVSSRQSTGASSKVRSLLIGGASVNIGFLPKPIGANDSIRNILITGHENGLVRILDVTKGEQEQMENIVQVGLRETLYDHGDPRSLRVKFVSCAFQNRELLVGLASGEVVICKFGKNTSRGTGIPQGKDYHDTPIHHQNGNAKLLDISGRISGSITSSASFLPTNLLQVEPSENISVMKMSKVGFAAIGYKSGRLVVCDISRGPAVILNLERVSEHLSTVTGNCYPTAIEFSIMEYGNDGYSSILLLVGTNCGGNLLVFKINPMGNGGFEVVFATRTIQLNYRTSDPANAEASCISEIIPINSEDGTSAVATMDNFTRLSQGVRIPGYVITTSDRDVRVLSLPKTKLSHKVVEDSCLKCSVVNFRDQGVVLAILVKSGFVKFCSLPALQDIVDIKLPKEVYSQVRGSLESGLAQQSNLLSTGELYVRSSKTESVYISVYEKVKFKGGDSTTDLLFNATAVIPPRPTASALSWAKGQASYMTTAELTALIAGPNRGHPKTKESELAFNISPEANTAQGYGGYGMQQQHHQNQQQQTQRPYDPPVRRSTAGSGGVGSQGFMRRIQDGIQQVEETFNDYANSVTENVTEGLADQKKSFYSSALKSKVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATV6</accession>
    <name>G3ATV6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_52426</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377303.1">
        <property type="nucleotide sequence ID" value="XM_007377241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATV6"/>
    <dbReference type="STRING" id="619300.G3ATV6"/>
    <dbReference type="GeneID" id="18871678"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52426"/>
    <dbReference type="eggNOG" id="ENOG502S680">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023689_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATV6"/>
    <dbReference type="OMA" id="CVYPKYM"/>
    <dbReference type="OrthoDB" id="4091477at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="490"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="564"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="496"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="525"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="603"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="770" mass="86731" checksum="808AC0986D231DBF" modified="2011-11-16" version="1">MTSSSRETHDYSNFGNHGPPYQQTEPYYPHFNAELNFKEETPVVFQNQNQSPHHTQFSPQQPQQPHNQRIHSQEYHRSGSGYFEGEVKPQNDDEDGLGYFSNRTSSSVPSSRIDHYYNDTLIQQQLAQLLQQQQNQQHHQHQQLNEQNQQQHHQNQTSQQHLSLPQQQPAQPAQPAQPSQPQQQNDQQYLLSHPHSNQNYQTTPYKRSPLSREQEWTPTNSQTMDNSIGNPIPSRGTSQDVKTSFPPRPQSIRQQSLPVFNKQSTNMTQVQPSLIRELAGESVNSLMGFNPQTIYYNKDNTIRVNPDPSGNETIHEKPSDLDVKPKAKRGRPKKNPGFFIKLDSINKRPVTRQVKSSQSDSGTDYWNRSSSGGLLHRALSFTPSQPIVQQGQEDPDLPSELQFLEQDLQLTDDPYGLNMPSFNLTPSAEPPKSVGAVPSNLPPSGYFELENSTTAVNTGQFFSPGIDGHENNINSFNQTFEDYLKSTQLKTDDDANSGTTKSFSEEQTNPITANFTEFEYGASLPLPPPPPPPPPPPPQQQQQQVPHQMLPQVPFFCEPVTSIDSSQAYGSSTNESFLSPPNDHQTNVSSGENSDHDHEDNYYLQTTNTLPRSGSNQSVSSGGSGNSGGEDIDIAFANTSGAGNTSKPKKRLSKGAKCSICGKYISRDMTRHIRIHNEVGRFQCVYPKYMCNHRTQYFNRPYDYKKHLLHSHFKFDDPKGKTAHTLTDKLPMLGGCAACGARFTASQWLDFHVLVNDRNQRCPYIEEKDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU05</accession>
    <name>G3AU05_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_157326</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377351.1">
        <property type="nucleotide sequence ID" value="XM_007377289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU05"/>
    <dbReference type="STRING" id="619300.G3AU05"/>
    <dbReference type="GeneID" id="18871074"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157326"/>
    <dbReference type="eggNOG" id="ENOG502SZUG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014396_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU05"/>
    <dbReference type="OMA" id="PPNYMAY"/>
    <dbReference type="OrthoDB" id="4082517at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="578"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="578"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="590"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="684" mass="77773" checksum="014DA0DEC8593C47" modified="2011-11-16" version="1">MPLPFTRQQIEAPLLNSVPSNIIPIIHPPATINDYFTDVRTDLTNTKLSNQVTGIIQKFPPPLPPFPPQSPQSDDELDDDERGGIMVPFIYPPPPIINPDTLPYSLASFKSRLEQVKSTMVRIFVQAPPLPFPPPNYMAYPLSANKSTLIPADVFQSFISASEELPRTDMVVASAAGSLFDMREQANDEGFNIEDFERTRRYNNPRSEQSYDDENESEESDEEDADGYDEDGEQLEYLNRIDTTDYLDPFENDNRRDVFQINMRSPAYETVSKKLSKLKDANYHEKTKSNRENEYEFAQGMTRSFLESLSPSDIIDNPGKDITLQSGSNRERRRSELVKSMNTVEEFNEENRDEIYRMKKNQLLNRLHNLRNSKITFTSVETEDEELDQFRQLAEIERDEQLIQLKLSENYELLKTSLVFYEDSNKAYKNLNLVLINKLEKLKNFFVYQRDLFTEYLERKQDIFDIKSKESQKLFNGISNRNYSQEIKNIIKSSIINEQEQHLKPVEIPDHDLTTLATDTSHVLVHDFMPLITPEEFSIITGDIPRKSKLSNNAKDNKPATNMKHHIFQNSLYDRMTSGSDTNASDANISSGAASGSGSTTAGPKRRGRRSAQSLQQAGGASGNTGNGSSKNSDTRDGLGVKYSEAALISKITKHFVGPQSAMPDELTNDLDMMGIQTRWPVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU51</accession>
    <name>G3AU51_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Lysophospholipase</fullName>
            <ecNumber evidence="2 10">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_143669</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the release of fatty acids from lysophospholipids. Phospholipase B may well contribute to pathogenicity by abetting the fungus in damaging and traversing host cell membranes, processes which likely increase the rapidity of disseminated infection.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the lysophospholipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="2 10"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377398.1">
        <property type="nucleotide sequence ID" value="XM_007377336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU51"/>
    <dbReference type="STRING" id="619300.G3AU51"/>
    <dbReference type="GeneID" id="18870562"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143669"/>
    <dbReference type="eggNOG" id="KOG1325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014602_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU51"/>
    <dbReference type="OMA" id="FGHINMS"/>
    <dbReference type="OrthoDB" id="4084751at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000010">
        <property type="entry name" value="Lysophospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF33">
        <property type="entry name" value="LYSOPHOSPHOLIPASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9 10" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6 9 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005131605" description="Lysophospholipase" evidence="10">
        <location>
            <begin position="17"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="PLA2c" evidence="11">
        <location>
            <begin position="29"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW30427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="600" mass="65403" checksum="117F53254AFB462F" modified="2011-11-16" version="1">MYLIPQILLLLQVVLATSPTNDYAPGSVSCPGGSLTRAATGINTQEENYITNRNPITKSALGTFLSNAGLTDFDVSSFLSQAQPSIGLAFSGGGYRAMLTGAGELAALDSRTWSKDKTLAGILQASSYITGLSGGSWLVGSVASNDNLSIDQILADGNLWNIQSILDYYGINVIGDTVMWSHIADQVNDKSNAGFETSITDPWGRALSYQLLTNFDDKGDAVLWSDITSSSAFSTFQMPFPILVADGRAPGTSIINLNSTVFELTPYELGSWDPSLKSFVQTKYLGTTLDNGQATGQCYNGYDNAGFFMGTSSSLFNEALLSLNQSGLPSFITDLINKYVVDPIENSNVDVADYNPNPFYKSTNQDTTISKASVLNLVDGGEDGQNIPIVPLLNRNVDVIFAHDNSDNINSWPDGSSLINTFERQFVQQGENVAFPYVPDQKTFRNLNLTSKPVFFGCDAQNLTSLTDDIYDVPLVIYLPNRPFTYWSNTSTFKLDYSDTERNGMIQNGYEISTRKNGTLDSEWAACVGCAIIRREQERLGQSQTPQCQQCFTNYCWDGTIYEGADLGDNYSDDGLTKSATYYNSKNVAGFDDGATNLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU80</accession>
    <name>G3AU80_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="6">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_63289</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="6">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="6"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377427.1">
        <property type="nucleotide sequence ID" value="XM_007377365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU80"/>
    <dbReference type="FunCoup" id="G3AU80">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU80"/>
    <dbReference type="GeneID" id="18874683"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63289"/>
    <dbReference type="eggNOG" id="KOG0335">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_18_0_1"/>
    <dbReference type="InParanoid" id="G3AU80"/>
    <dbReference type="OMA" id="HSTIDFI"/>
    <dbReference type="OrthoDB" id="10256233at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990400">
        <property type="term" value="F:mitochondrial ribosomal large subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902775">
        <property type="term" value="P:mitochondrial large ribosomal subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016070">
        <property type="term" value="P:RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="164"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="414"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="574" mass="65156" checksum="57EB32E527C41C76" modified="2011-11-16" version="1">MISILKQCQSIRYSQVSPVLSLAKRFASKRPAHKKYSRPKAVSKSAVDKNDDAQKKAESIFSFGKFSQLHTPSLKERESASQAIAKIHEFDQLRIFPSVRKAMLEEIKSQYNMKGKTESDLHLEPTPVQISAIRKVNQSRDIKLPENFEELSEGERIALTISQENQANRLKVFTIAAETGSGKTWAYLASVLSKLKEDDMNVWQESPAKYQQSKDHAIIRSVILVPTHQLVEQVYDTLARATTIKLDHSKNVHPQFKPFLELDENKTLGLSVMKWSQGDPPTTLFDRCEQGRLDVLVTTPAKISSLSNLRNFTRPLKYFNSVRYCVIDEADTLFDQSFIADTSFVLKHMPRLVDLILVSATIPKEFQKILKAKFPDQKSLINVQTPQLHKIPRRIKVFTLDAELPPYNGSKTRCLAQALYAIAKDGTDPGYVKRIVIFVNKRDEVKPLVKSMTEKFHHKPHDFYGITQDLSKEERDKLLKPFLQPAGLIEDDEHKSKYKVLVCTDALARGVNFIGVKNVVMIDLPHNSVDLVHRIGRTGRMKQNGRVFVIVDRKTRKSWIKGLGNAITKGATIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUE3</accession>
    <name>G3AUE3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">VPS9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_157657</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377490.1">
        <property type="nucleotide sequence ID" value="XM_007377428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUE3"/>
    <dbReference type="FunCoup" id="G3AUE3">
        <property type="interactions" value="160"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUE3"/>
    <dbReference type="GeneID" id="18871093"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157657"/>
    <dbReference type="eggNOG" id="KOG2319">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007625_3_3_1"/>
    <dbReference type="InParanoid" id="G3AUE3"/>
    <dbReference type="OMA" id="ARYTKGF"/>
    <dbReference type="OrthoDB" id="300289at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14369">
        <property type="entry name" value="CUE_VPS9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041545">
        <property type="entry name" value="DUF5601"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045046">
        <property type="entry name" value="Vps9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041804">
        <property type="entry name" value="Vps9_CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101:SF25">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN AND VPS9 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101">
        <property type="entry name" value="RAB GDP/GTP EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18151">
        <property type="entry name" value="DUF5601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00167">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="VPS9" evidence="5">
        <location>
            <begin position="200"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="CUE" evidence="4">
        <location>
            <begin position="478"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="370"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="338"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="523" mass="59419" checksum="CC837370EA0524B0" modified="2011-11-16" version="1">MTMQPYTPPLDNGSSITSIHNSGNTTSNNNNDNLIDLSDSENTQQEGQPQPSPQAVPQVPTQAPHPEATRHASIHVDVNPSEQYQQSHRPFDFHVFLSHLRKKSADPLVRYIRSFLSSYIRQGYTFSAEQRIKIIIDFKQFMNDKFAMYEPFASMDDIDLENSREGLEKLVMNRLYDQCFPPEVVKTNPQFMPDSYTRDLILDKEFETTLEKFSWINGTHLDIDLDELGTDSTNFLDHAINELNKINQYRAPRDKIICILNACKIIFSFLKSTNKETNADAFVPLLILVIFKAKTSNLISNIHYIENFRGEEWVNRGETSYYLSSIQGAIGFIKNLGVDELTITNEEYDAHMEAWEAERKQREVEIKINQTDKTDSGGDGLLVQPQPQHIPSTPMRSADYSQQGLSPSSVLFSSAEMLGKSISNFLSPSATPPSQDQQQSQAVVPAVSQNMIDEFQNLPDNQEYQPPPPPENEINHQQLSEAYDTLREVFPNLDKNILKDIIYLNRGDVDVCIDACLQLVADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUI4</accession>
    <name>G3AUI4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alanine--glyoxylate transaminase</fullName>
            <ecNumber evidence="3">2.6.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_143334</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.44" evidence="3"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377511.1">
        <property type="nucleotide sequence ID" value="XM_007377449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUI4"/>
    <dbReference type="FunCoup" id="G3AUI4">
        <property type="interactions" value="1391"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUI4"/>
    <dbReference type="GeneID" id="18870536"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143334"/>
    <dbReference type="eggNOG" id="KOG2862">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027686_5_2_1"/>
    <dbReference type="InParanoid" id="G3AUI4"/>
    <dbReference type="OMA" id="YEWDTPA"/>
    <dbReference type="OrthoDB" id="7403325at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008453">
        <property type="term" value="F:alanine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004760">
        <property type="term" value="F:L-serine-pyruvate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019265">
        <property type="term" value="P:glycine biosynthetic process, by transamination of glyoxylate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000049">
        <property type="entry name" value="Alanine-glyoxylate aminotransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000027">
        <property type="entry name" value="Serine--pyruvate aminotransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024169">
        <property type="entry name" value="SP_NH2Trfase/AEP_transaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152:SF24">
        <property type="entry name" value="ALANINE--GLYOXYLATE AMINOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152">
        <property type="entry name" value="AMINOTRANSFERASE CLASS V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000524">
        <property type="entry name" value="SPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="11">
        <location>
            <begin position="35"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000524-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000524-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW30540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="381" mass="41111" checksum="40FB37510A0C12A8" modified="2011-11-16" version="1">MSSTTSFVQPDHRLTLIPGPIEFSNPVLAAMSTPSQAHTSPEFIATFQSVLKNLRKLFKSTNPKTQAYVLSGSGTLGWDIAASNLLTAGDDVLVLSTGFFSDSFADCLRIYGAEVDVVTAKVGDVVPLDQVKAKLSEKSYKAITITHVDTSTSVVSDVEAISKLVKQVSPETLIIVDGVCSIGVEDLEFDQWGIDFVLTASQKAIGVPAGLSILYASERALDIALNKKETTYFASLKRWTPVMQSYESGKGAYFATPAIQTITALRASLDEILSEPLDARFAKHEQVSTEFKNAVNKLGLQILPVSRDVAAHGLSAVYFPEGIDGNELLKKLAQKGFTVAGGIHKQLVGKYFRVGHMGYSVYAGHIPAFTKALEESLKELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUT2</accession>
    <name>G3AUT2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Guanine nucleotide-binding protein subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_144386</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G protein gamma family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377790.1">
        <property type="nucleotide sequence ID" value="XM_007377728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUT2"/>
    <dbReference type="FunCoup" id="G3AUT2">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUT2"/>
    <dbReference type="GeneID" id="18870623"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144386"/>
    <dbReference type="eggNOG" id="ENOG502S5Z5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163540_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUT2"/>
    <dbReference type="OMA" id="TECCTIM"/>
    <dbReference type="OrthoDB" id="59470at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041848">
        <property type="entry name" value="Ste18_fungal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28189">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28189:SF1">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="G protein gamma" evidence="10">
        <location>
            <begin position="10"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="88" mass="10550" checksum="2A095BF04A5D65BB" modified="2011-11-16" version="1">MNHEVNEQIQILKLKRIKELINRLEESLNRERIPASNACELIINYVEETPDYLIPYNWKLPPERNKFAQYQKYQMMKPKRPSGCCTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV13</accession>
    <name>G3AV13_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63711</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377853.1">
        <property type="nucleotide sequence ID" value="XM_007377791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV13"/>
    <dbReference type="GeneID" id="18874878"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63711"/>
    <dbReference type="HOGENOM" id="CLU_2484748_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV13"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="87" mass="9726" checksum="91D2967F585F162D" modified="2011-11-16" version="1">MSSAAIPKGPPPPYSEETQTDIKTHQPLQTNQDSNKSSNQTENKKSCPWSILKRKRTLMAMGGVYWWPMGGCCCHKKRNSRKNSSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVD1</accession>
    <name>G3AVD1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_63759</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377916.1">
        <property type="nucleotide sequence ID" value="XM_007377854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVD1"/>
    <dbReference type="STRING" id="619300.G3AVD1"/>
    <dbReference type="GeneID" id="18874899"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63759"/>
    <dbReference type="eggNOG" id="KOG0015">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063931_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVD1"/>
    <dbReference type="OMA" id="AGHAHHI"/>
    <dbReference type="OrthoDB" id="2284405at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00266">
        <property type="entry name" value="MADS_SRF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000002">
        <property type="entry name" value="Serum response factor b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033897">
        <property type="entry name" value="SRF-like_MADS-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="51"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="132"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="203"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="143"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="163"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="266" mass="28144" checksum="0852804A278157FB" modified="2011-11-16" version="1">MSSVTDPKIEGESHFDTGSNGDPANAGGIASVGGADDDDDDDGPSGGKTQKERRKIEIKFIQDKSRRHITFSKRKAGIMKKAYELSVLTGTQVLLLVVSETGLVYTFTTPKLQPLVTKSEGKNLIQACLNAPEEGLGDDQGDQSDGNSGDSPDQSPAPNSLPQVQAQQQQQQQQAAAAAAAAAQAHHVQQQQAAAVQQQQAAAAAAQQQLPPGAHIPHGMPYPSAGGHPGQPGGMPMPQNYGDSQVYQQYFSSMQNSNIQNQQQYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVF5</accession>
    <name>G3AVF5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="2 8">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_57614</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the phage and mitochondrial RNA polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2 8"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377940.1">
        <property type="nucleotide sequence ID" value="XM_007377878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVF5"/>
    <dbReference type="STRING" id="619300.G3AVF5"/>
    <dbReference type="GeneID" id="18872137"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57614"/>
    <dbReference type="eggNOG" id="KOG1038">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007518_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVF5"/>
    <dbReference type="OMA" id="LVKRPVM"/>
    <dbReference type="OrthoDB" id="276422at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034245">
        <property type="term" value="C:mitochondrial DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001018">
        <property type="term" value="F:mitochondrial promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006390">
        <property type="term" value="P:mitochondrial transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1320.10">
        <property type="entry name" value="DNA-directed RNA polymerase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046950">
        <property type="entry name" value="DNA-dir_Rpol_C_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002092">
        <property type="entry name" value="DNA-dir_Rpol_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037159">
        <property type="entry name" value="RNA_POL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029262">
        <property type="entry name" value="RPOL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00940">
        <property type="entry name" value="RNA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14700">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01311">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00900">
        <property type="entry name" value="RNA_POL_PHAGE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00489">
        <property type="entry name" value="RNA_POL_PHAGE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase N-terminal" evidence="9">
        <location>
            <begin position="155"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1073" mass="124327" checksum="0362C58D0F611D7A" modified="2011-11-16" version="1">MTKEKVGIAFAKATIHLYYIANRYSVTKRISPFLVDFLFDNLADFKLELQSVASELSEQYLRDCIITICRRKKITIPTFKQSQPVHDNKFQIALSKYKNEYGSISIHELYKFIQETKFDWNGKIKPNIKLYEFYDTLNARDKHEFMQAYLNFHTEKQAIIEEAMDFIEYIPSEFKQKSNNLISLVYNNNELLNQWISKTSTKIMNMLQVKNPETDQEKLLHQYQSYFVFVPVSLHIHGLLSEILGNVGLYNDGVPIYKIKSQLRYSFDKQMVNAIPFKHNGGIMKYMNRNDRDDLISVFLDVLIESATIEISEDNIKLMKTMTKDVTSEEFLNRNEDGSCPALIVCDSEGSYKYPVIKIHPILENDLSNCRGKVSRFPMVVPPKPWTNLFEGGYLVHKECVFMARFDKTYLNYFKQVSYDGHLDSVFMTLNKLGSLAWAINPDMLKILNRAANMPQGFLKIPRPISDKVPKFKDKFAQQKYISSKGARITFNMLLDMANGFSKNGEMLYTPYQLDFRGRVYPSSNLSHILSDEFRCLFMFWHSKALGNDGFNWLKYHLSSMFGNSGFHMEQAIGFCDKHKQDIIDSAKYPFDGKQWWLKSKTPYQTLSACMELTKVWEFEEQGGNIEEFRTRFPVHQDGSCNGLQHYAALTRDERGGKAVNLLDEPKKQDIYMEVANIVESKLQALIDSPDETERLYAKLGLIVLSRKLVKTTVMTTVYGVTTIGAAGQVRNRIKDFVDDCNQNPQMKGTISEEALEKIQSESFKLGMFLANVILGSISSLFAPATAVQQWLRVNIKRVLSSFNSKSVMYIKNMKPELFEKVFTQPQSLRPIIWKVPSGFPVIQYYVKSPNNQNFQSVLSSVVASQGKRTLSPMSKYKSVNGIAPNYIHSLDAVHMMWTCNNMYNEGLSFAAVHDSFWTHPCDVKRMSTILRQEFVKLHTPDLLMSLRNDLMQQVKGSYQLVQFRSCDFPELAKRIKRLRQTYPCRDNLNEMLYYELTQLEQGTSEISLWIEDYDPKVYFNREEYNSSPQVPEKYVNNGREKTWVFVPVKIQDLPAKGKLDLNRVLQSEFFFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVH2</accession>
    <name>G3AVH2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HBS1-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_144545</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377688.1">
        <property type="nucleotide sequence ID" value="XM_007377626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVH2"/>
    <dbReference type="STRING" id="619300.G3AVH2"/>
    <dbReference type="GeneID" id="18870641"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144545"/>
    <dbReference type="HOGENOM" id="CLU_176450_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVH2"/>
    <dbReference type="OrthoDB" id="342024at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015033">
        <property type="entry name" value="HBS1-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08938">
        <property type="entry name" value="HBS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HBS1-like protein N-terminal" evidence="5">
        <location>
            <begin position="9"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW29922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="89" mass="10884" checksum="9D3F955686684FBC" modified="2011-11-16" version="1" fragment="single">TMDYDEDDLDYPSEEDYEFHEDNLSNEDYEKLHKYLPQLKDIMSEYDADEYDLKESLYFNYFDVSASVQELKSKFKKSMYNLFLLSRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVN5</accession>
    <name>G3AVN5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 19">3-isopropylmalate dehydrogenase</fullName>
            <ecNumber evidence="7 19">1.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">SPAPADRAFT_63812</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17 19">Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:32271"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.85"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="19">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="19">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 19">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 19">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.85" evidence="7 19"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377966.1">
        <property type="nucleotide sequence ID" value="XM_007377904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVN5"/>
    <dbReference type="FunCoup" id="G3AVN5">
        <property type="interactions" value="917"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVN5"/>
    <dbReference type="GeneID" id="18874928"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63812"/>
    <dbReference type="eggNOG" id="KOG0786">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_0_3_1"/>
    <dbReference type="InParanoid" id="G3AVN5"/>
    <dbReference type="OMA" id="EYDLGAR"/>
    <dbReference type="OrthoDB" id="419183at2759"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00072"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003862">
        <property type="term" value="F:3-isopropylmalate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000006">
        <property type="entry name" value="3-isopropylmalate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004429">
        <property type="entry name" value="Isopropylmalate_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00169">
        <property type="entry name" value="leuB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42979">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42979:SF1">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="16 19" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9 19" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="11 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 19" id="KW-0520">NAD</keyword>
    <keyword evidence="13 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="20">
        <location>
            <begin position="7"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00019276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU004445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EGW30200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="374" mass="40077" checksum="B8F5EDE67E31350F" modified="2011-11-16" version="1">MSKITKTITVVPGDHVGTEICDEAIKVLKAIESSTPYHNIEFDFKHHLIGGAAIDATGSPLPDETLAAAKQSDAVLLGAVGGPKWGTGAVRPEQGLLKIRKELNLYANIRPCNFASDALLELSPLKAEVVKGTNLIIVRELVGGIYFGERLEQEQSEDKETAWDTEKYTVAEVTRITRMAAFMALQHNPPLPIWSLDKANVLASSRLWRKTVDKVIAEEFPTLSVQHQLIDSAAMILIQNPTKLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDTNTAFGLYEPCHGSAPDLPPNKVNPIATILSAATMLRLSLDCVKEAEALEEAVKQVLDSGIRTGDLKGTNTTKEVGDAVAETVTKILKAAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVN8</accession>
    <name>G3AVN8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sister chromatid cohesion protein DCC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_158781</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DCC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377968.1">
        <property type="nucleotide sequence ID" value="XM_007377906.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="G3AVN8">
        <property type="interactions" value="429"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVN8"/>
    <dbReference type="GeneID" id="18871134"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_158781"/>
    <dbReference type="eggNOG" id="KOG0798">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034504_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVN8"/>
    <dbReference type="OMA" id="DSESWPF"/>
    <dbReference type="OrthoDB" id="276989at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035753">
        <property type="term" value="P:maintenance of DNA trinucleotide repeats"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034088">
        <property type="term" value="P:maintenance of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034398">
        <property type="term" value="P:telomere tethering at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019128">
        <property type="entry name" value="Dcc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395:SF6">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09724">
        <property type="entry name" value="Dcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="349" mass="40155" checksum="6523AD01B278B3EF" modified="2011-11-16" version="1">MYQLYQQINKDDKHTYKLLQLPPDLLTHLSTSSDSELLIKSDADASSVVICNNDTTWKLRQMNHSNTVVLLDNLNIKHRDTSLSETTNVLIGGDMCSYEYELTSTKGVINVDKIPVYDGDELQESITLDELSYDSLCSKNEFVNNWYELGGCEIMGKAYLLSPRLITEILYVIITYLISRKINYNNSFNLQDIKVVDENINESMVFSVLHKFGQVADNEVTLQNDKIAKWFGVIELAKSDDLINANDFLINWKGSLPPFYNVSLDIADLRGYYSSPILNKMRYVNPKLLSTNLSTRFSQLFEIDNNWDYDLFVPLIEEFVPKGKKIDTLIIKYARKKKITKNKFTVCPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVT3</accession>
    <name>G3AVT3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein AF-9 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_158257</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377744.1">
        <property type="nucleotide sequence ID" value="XM_007377682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVT3"/>
    <dbReference type="FunCoup" id="G3AVT3">
        <property type="interactions" value="736"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVT3"/>
    <dbReference type="GeneID" id="18871120"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_158257"/>
    <dbReference type="eggNOG" id="KOG3149">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051385_2_1_1"/>
    <dbReference type="InParanoid" id="G3AVT3"/>
    <dbReference type="OMA" id="VKPYHNE"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16908">
        <property type="entry name" value="YEATS_Yaf9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1970">
        <property type="entry name" value="YEATS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038704">
        <property type="entry name" value="YEAST_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005033">
        <property type="entry name" value="YEATS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055129">
        <property type="entry name" value="YEATS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47573">
        <property type="entry name" value="PROTEIN AF-9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47573:SF1">
        <property type="entry name" value="PROTEIN AF-9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03366">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51037">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="YEATS" evidence="7">
        <location>
            <begin position="6"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="188"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW29978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="221" mass="26166" checksum="E9C16BA19A5DE141" modified="2011-11-16" version="1">MSHARRIKHISISVPIMYGNHAVRLTDDMRKPTTPPDHTHEWTVFFKPVLNDIDLTPLIKKVTFKLHETYENPVRSIEHPPYQVTETGWGEFEIIIKLHFNSNVEFGINEKNFQIFHALKLHPFNPQVPVKPDGEVNSVLYDELVFNEPTDRVFEILTRKPVNLLPYELSNPDKRDQEYIRLDEIDEVNRLEVYINKVKDEIENQRDQYKDLEQQKLALLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE50</accession>
    <name>G3AE50_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58806</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372996.1">
        <property type="nucleotide sequence ID" value="XM_007372934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE50"/>
    <dbReference type="STRING" id="619300.G3AE50"/>
    <dbReference type="GeneID" id="18872616"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58806"/>
    <dbReference type="eggNOG" id="ENOG502RXZN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1639516_0_0_1"/>
    <dbReference type="InParanoid" id="G3AE50"/>
    <dbReference type="OrthoDB" id="2100128at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39398:SF1">
        <property type="entry name" value="CSN8_PSMD8_EIF3K DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39398">
        <property type="entry name" value="YALI0F14311P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="162" mass="18729" checksum="CCF23EE4EA6AB465" modified="2011-11-16" version="1" fragment="single">MTPPEYHKKYVPPNRRNNKKPQQDNKDNKREQRKARFANNSSSSSSQSSYGFVSRGEDNRLQQSAYDRERFFISIIGQFIQFNSQTQIGDIRSLINEGDLQTANAEVMTPPADKITMDSILLSIRKLREALLYTKPTPFHKKVFLFSLRLAAIVGHYQTYIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE54</accession>
    <name>G3AE54_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_131427</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373000.1">
        <property type="nucleotide sequence ID" value="XM_007372938.1"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AE54"/>
    <dbReference type="GeneID" id="18869574"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131427"/>
    <dbReference type="eggNOG" id="ENOG502QRR7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_232139_0_0_1"/>
    <dbReference type="InParanoid" id="G3AE54"/>
    <dbReference type="OMA" id="VWYNSLR"/>
    <dbReference type="OrthoDB" id="2149224at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053005">
        <property type="entry name" value="Nuclear_Pos-Cytoskel_Interact"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28190">
        <property type="entry name" value="NUCLEAR MIGRATION PROTEIN NUM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28190:SF1">
        <property type="entry name" value="NUCLEAR MIGRATION PROTEIN NUM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="78"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="2052"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2052" mass="229830" checksum="F4771935DA1543CC" modified="2011-11-16" version="1" fragment="single">MPPTDKSSSSSAKDDIRGQLSDIQRKLFSRSSSKQSSPVHQNAARTVTGKRTISTSSDQENLAPHFPPPKLGSPVGRSLTPPAASGAGAAAADLGTSSSSRTGSPRSASSSSKKSHHHGSPLTMIQPSSPQTKSLLGPESKGNDVNFVVGLSENLLSECRRLTAENAKLKPKLKSALEEIQTFKDEIFELKHYKEFQATNENQLKDKNWELEATVNNLNDQINQLNQENSKLAGKNDELSTELANVNKQHDKLNVTNESLIKNLNDLQLNYQAQITNLNQRIDALNDENDQLHLKLTGPDQSTSTDSVKATAKNDPWLNLDDSTNGGDDGANDMTFDSILLELEAHDLPPSNPSNPQLEIETLRSTLQHSNRTIAKLRSSILKMKREDLQSTPSPTKSSLRFRKSRNEVQPLGTSSNRNSMISPKKRDSKFLITADEDTSFTEFPTEGGEWDDFIEDIPSTTKKSISPVRTNASPVRSNVLSDVPQLVESEGEEEDTTIQRKPTILSKELSTKSIEEQIQEYVKANNLILIPVDQYTDLSNNSITTLTNDKIVNLAKAKGYHVLSQADYEELVNEEEMKKKLEAKGLITIPQDDLTKLKHDLDSPSVYLKEKANSLGLIMVSSELYDEVVKNANEPTVEHITSKAKDLNLHVVPNEDYEKLTNPTIEDLSHHAEKHQSKVISTNEYEGLINPSAQAVEEHATKHNMKAITIDQLTSLETSIAKPTLEYLKTHAPNYNHQIIDTEELAKLNKLANEPSLQQVKLHADKHDLVTLSKPEYTELFKMANEPTVENLTTKANDKGMTVIKTSEYQTLTNPTVEQLRDKAGGFHHAVIDNDQLQALKEMVDTPDIEHITSKAQAMDYKVVANSEYTKLHQRATNPTLNQVNEMAQALNQSVIPTTQLNDLNSLAHEPTIEHLQSMAEKKNHTLISKESFENLNKLAHEPEIDHLTKLASDKQHVIIPQSTHDELLNPSIYVVSQKAQELGHVVVPESELVELTTKANEPSRDHLAAKAKNIGCSIIDDSELEDLTTRAERPDLELIKNHATTLNHSVISNQELQELKKTVDSPSVDHIIAKSKDLGYIAIPQEQHEELTKLAYEPSVDTVKEHVSKHGLVAIPVQEHEKLKQISETPDIGFIKEKASMLDSVVVPDKEFANLQTLANNPPLDHLKSKVETHDHIIIPASELNELKSLIASPSLEYMHEKSEAQNMKLVPVDEFKSLLDIAHNPSTSHLESKLMNLGMAMISVSALSELKHKLAEPSEEYLREKANSRQLQVVHNDELARLHHELNEPSDEYLKNKSEARGLQLVPSEELARLHHELEEPADEYLKTKSEAREDLKRMEHEINEPNIEYLTNKAHSHQMNLIPVDELTKMKQTIESPAEEHLATKAQSKGLQLVKNDDLETMKNTIENPSVEFLTEQASAKGMSVIDTREYVSLKQDLEQPTHEYLSRKAQQQGKQIVEDNEMESLKKLAHDPEIDHIKNVCSNKGLVVLPTAEHKKLVEQASNPDISHITKVASKRDLVVLPVVEHKDIVERSTNPDVSHIETVAAKRNHKVVPNMEYDKLIHKAEKSLEEHAKQEGKEVIATKDLQDLKDGIENPTLEYMKECCRKHLHKLVPEEEYSALQVKANRPVEEIARESGLAVLPSEKLQELERNVESPTTEYITNKAASLGLVAIAAKEYSNLTNYFKKPIEEIANDSNKVAVETTEYQRLITQSNEPSLDHIRSVAQKHNHVPIPKEEYEGLTKNTQDPDLTFVETKANDKGYKLIEQNAYDDMLEKVNRPIEITAKELGLSVVPIQELEDLKTQLQSPSEDYLQEKAKSLDAVVVSTNELNSLKELVNEPLEQKAGAVGMAVVASGKLAELEEVYNNPTIEFLKKKISELGFEIIEAKDLDNLRQESTDPSIERIKLHAENKENSVKLVPLEDFEQMEKLAKESLEEKAEKEGKKVVDVDKWEKIYQDATNPSLEQISKCAKQKSSVVIPEEQYIAMTKELETPIENKAESVGLKVLPLAIFADLMKHKESPSLEFLKDKSSSSGHEIVEKSHLEHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEL8</accession>
    <name>G3AEL8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation factor Tu</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_57846</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">G-protein that, in its active GTP-bound form, binds to and delivers aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. In the presence of a correct codon-anticodon match between the aminoacyl-tRNA and the A-site codon of the ribosome-bound mRNA, the ribosome acts as a GTPase activator and the GTP is hydrolyzed. The inactive GDP-bound form leaves the ribosome and must be recycled before binding another molecule of aminoacyl-tRNA. Required for mitochondrial protein biosynthesis and maintenance of mitochondrial DNA.</text>
    </comment>
    <comment type="function">
        <text evidence="10">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372192.1">
        <property type="nucleotide sequence ID" value="XM_007372130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEL8"/>
    <dbReference type="FunCoup" id="G3AEL8">
        <property type="interactions" value="890"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEL8"/>
    <dbReference type="GeneID" id="18872221"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57846"/>
    <dbReference type="eggNOG" id="KOG0460">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007265_0_1_1"/>
    <dbReference type="InParanoid" id="G3AEL8"/>
    <dbReference type="OMA" id="EGDKEWG"/>
    <dbReference type="OrthoDB" id="2067at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070125">
        <property type="term" value="P:mitochondrial translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01884">
        <property type="entry name" value="EF_Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03697">
        <property type="entry name" value="EFTU_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03707">
        <property type="entry name" value="EFTU_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000001">
        <property type="entry name" value="Elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000003">
        <property type="entry name" value="Elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_B">
        <property type="entry name" value="EF_Tu_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041709">
        <property type="entry name" value="EF-Tu_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033720">
        <property type="entry name" value="EFTU_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004541">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_bac/org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00485">
        <property type="entry name" value="EF-Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000766">
        <property type="entry name" value="PRK00049.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009372">
        <property type="entry name" value="PRK12735.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009373">
        <property type="entry name" value="PRK12736.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF36">
        <property type="entry name" value="ELONGATION FACTOR TU, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 12" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="46"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW34780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="438" mass="47913" checksum="B7342D62988A5C93" modified="2011-11-16" version="1">MLANFASLYRLSTVGGAAARTTLRTAALRSAYPSLVRSYAAFNRSKPHVNIGTIGHVDHGKTTLTAAITKVLSEKGGASFMDYGSIDRAPEERARGITISTAHVEYETDNRHYAHVDCPGHADYIKNMITGAAQMDGAIIVVAASDGSMPQTREHLLLARQVGVQQLVVFVNKVDMIDDPEMLELVEMEMRELLSQYGFDGDNTPVVMGSALCALEGKKPEIGSEAIMKLLDAVDEWIPTPVRDLEQPFLMPVEDVFSISGRGTVVTGRVERGTLKKGEEVEIVGNFDKQFKATVTGIEMFKKELDQAMAGDNCGILLRGVKRDEVNRGMVLAKPNTAKAHTKFLASLYILSTEEGGRHSPFGENYKPQAFFRTTDVTCAFKFPPGEGVDHSQQVMPGDNIEMVGELFKKTPIEVNQRFNIREGGKTVGTGLITRILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AER3</accession>
    <name>G3AER3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enhancer of mRNA-decapping protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_58894</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EDC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373101.1">
        <property type="nucleotide sequence ID" value="XM_007373039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AER3"/>
    <dbReference type="FunCoup" id="G3AER3">
        <property type="interactions" value="157"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AER3"/>
    <dbReference type="GeneID" id="18872664"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58894"/>
    <dbReference type="eggNOG" id="KOG2585">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037134_0_0_1"/>
    <dbReference type="InParanoid" id="G3AER3"/>
    <dbReference type="OMA" id="AMFDKKS"/>
    <dbReference type="OrthoDB" id="10030313at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="entry name" value="YjeF N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025762">
        <property type="entry name" value="DFDF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019050">
        <property type="entry name" value="FDF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13612">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13612:SF0">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09532">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01199">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="YjeF N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51512">
        <property type="entry name" value="DFDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DFDF" evidence="7">
        <location>
            <begin position="132"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="YjeF N-terminal" evidence="6">
        <location>
            <begin position="254"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="99"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="524" mass="58686" checksum="0F62281D1A8D83E9" modified="2011-11-16" version="1">MKSVVANKKLGSKLVYSVYPSLAKINRVYNMTEFLNYKVNLTLKDGTKSTGIITHVDPQQIILNNASQSISPDQLLSNYSVKNTDILDLKVVQLPPNFKKKNKQQHQPPVSAGGSVSVNSTPRGKSPDWDSNELDDVKATGADFDFEANLAMFDKKSVFADFQKMDHVRPHDRLVGHNKLENVNKQKKKEKYDNNEMVLDRNRSDNWDLIGSHSTTNTNTPVIGKDHTSGVAAQSKFVNSSNGTVLPLATPVQLLEIERLSVEYGISANIMAEICGTNLSQLIINKILGGETRLNKLNHNLPPLVLLLIGSSRCGSRALATGRHLTNHGVRVLAFMINSPSQEQDEQDEDRDHLNLQLRLFESNGGKLVVNNFNELLDIITNQLDTPVELIIDALQGYDDHLEDIYYEQKDQIVLRQLINWVNEPHQHSRLMSMEIPSGIDGGSGIPSDETLVVKSKWCISMGFPISGLILAYKNNQLEVEEVGHYLLDIGIPNKVYSTKTNLRKFDKFWYDNAQSVIKMEVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3AES5</accession>
    <name>G3AES5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58904</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373113.1">
        <property type="nucleotide sequence ID" value="XM_007373051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AES5"/>
    <dbReference type="GeneID" id="18872671"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58904"/>
    <dbReference type="eggNOG" id="ENOG502QVKH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1062324_0_0_1"/>
    <dbReference type="InParanoid" id="G3AES5"/>
    <dbReference type="OrthoDB" id="4046837at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="262" mass="29852" checksum="8A11211927102535" modified="2011-11-16" version="1">MTRFIKSEDIDNHSYLLMFHRLFSIDPALLSGDDYVKALDIINFKQEKELSGKILDYINESKIDQEAENLRLRVKILECLGIADDNIEVALQKYHQYRTHATYGLHIVSTAMVKVFGYQAIKQDKNIYSTIAATLVPQDTITVKILQTLIVTKGYFDKDSALELFNDYINQVSADVNQATRRSAKGLLTEAVCLSSLQNNDRSFAQLVFDKAIDNNVISDEHEIAAVKKVFKAYGDSFIEDDDWSKAKVNMNSYVLNYIKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF78</accession>
    <name>G3AF78_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein FMP25, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_131380</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372279.1">
        <property type="nucleotide sequence ID" value="XM_007372217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF78"/>
    <dbReference type="FunCoup" id="G3AF78">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF78"/>
    <dbReference type="GeneID" id="18869569"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131380"/>
    <dbReference type="eggNOG" id="KOG1426">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028610_0_0_1"/>
    <dbReference type="InParanoid" id="G3AF78"/>
    <dbReference type="OMA" id="YDQPHEI"/>
    <dbReference type="OrthoDB" id="10256179at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053245">
        <property type="entry name" value="MitoProcess-Associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47563">
        <property type="entry name" value="PROTEIN FMP25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47563:SF1">
        <property type="entry name" value="PROTEIN FMP25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="427"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="667" mass="74923" checksum="4D0ED3F5A0C5CC6A" modified="2011-11-16" version="1">MLSGTIRASRRRFLFSRLSKVTVPVRLYSNPQDKKQTIEQFNKSKVDYKFGYNFDTIDDLDKKINPDRQATIDLEDELAANFNTRIENDPSLANLRYGSPEYKEQLHNLYEEFKKKQEKDRKRFEFNERMKGVGMGVLIVVGAISAHQIFMNYQYLKTSIMADYTYKIGDSKAQDLSAPELNTKRVANFAEKLESELNDETIANLQNSTEVPGLYVFGKHNGQKLPIRLPFFNGMLLKDVYISPDYLVVVNEKGQVYQWYKGLETPQLMNVPINVKQVCVSGDYLYMLSEKGEVVYTPRPEVKSAEFTPLKTRNLLGIQKEQTYNKVDVPGAVQQISSGKDHLLCLSRKGKAYVINTSSTPENYGQYGPAFSPFDKSVEIPCNKAIDITLLNNELITKKDGSKSVKERTFTSVAAGSNFNIVADQAGEIWTWGQNSNGECGIELNLKNELQPVPKKILSKKDIIRAFFIHLPKNVTTDNLTIDKVLAGNESSYVLVSYIDPEDSTQNKQVVLSFGNGVNGQLGNNRYLHVCSKPQIMKSLLGLEEYDEAKNALTNIGIKDISVGDKHLFVTLDNSGTNKDVLAVGENESGQHGNGKRVKTNKPVQLPKLIEPEDFEGQTRKKLVQKVGDVVTNRLSLLSGTQLPNGTNIEQVIVAGKESSAIFYRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFI2</accession>
    <name>G3AFI2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Succinate dehydrogenase [ubiquinone] cytochrome b small subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_58095</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the CybS family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372383.1">
        <property type="nucleotide sequence ID" value="XM_007372321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFI2"/>
    <dbReference type="FunCoup" id="G3AFI2">
        <property type="interactions" value="254"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFI2"/>
    <dbReference type="GeneID" id="18872319"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58095"/>
    <dbReference type="eggNOG" id="KOG4097">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096618_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFI2"/>
    <dbReference type="OMA" id="HSCITDY"/>
    <dbReference type="OrthoDB" id="18577at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098796">
        <property type="term" value="C:membrane protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03496">
        <property type="entry name" value="SQR_TypeC_CybS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1300.10:FF:000007">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] cytochrome b small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007992">
        <property type="entry name" value="CybS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13337">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13337:SF2">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] CYTOCHROME B SMALL SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05328">
        <property type="entry name" value="CybS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <note>ligand shared with SDHC</note>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>a ubiquinone</name>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <note>ligand shared with IP/SDHB</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR607992-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR607992-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="155" mass="16853" checksum="D4A8691CEE4BF960" modified="2011-11-16" version="1">MLSVYSRIGLNQSARLLIKPALIRGIKTIPQPPGYIVGTVNDAYVPPPPSKSEGSLHWTAERIVAVGMVPLVLAPLTTGTTTLLDSAMSSLLLFHCFAGFQSCIIDYIPKRVYGAYHTYCMWLLMGGTAFAGYGIYEIEKKEGGISNVVSKLWKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFL4</accession>
    <name>G3AFL4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pru domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_58128</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372415.1">
        <property type="nucleotide sequence ID" value="XM_007372353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFL4"/>
    <dbReference type="STRING" id="619300.G3AFL4"/>
    <dbReference type="GeneID" id="18872334"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58128"/>
    <dbReference type="eggNOG" id="KOG3037">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041798_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFL4"/>
    <dbReference type="OMA" id="CNTGRVI"/>
    <dbReference type="OrthoDB" id="340431at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2020.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.70">
        <property type="entry name" value="Proteasomal ubiquitin receptor Rpn13/ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006773">
        <property type="entry name" value="Rpn13/ADRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044868">
        <property type="entry name" value="Rpn13/ADRM1_Pru"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038633">
        <property type="entry name" value="Rpn13/ADRM1_Pru_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038108">
        <property type="entry name" value="RPN13_DEUBAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225">
        <property type="entry name" value="ADHESION REGULATING MOLECULE 1 110 KDA CELL MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225:SF0">
        <property type="entry name" value="PROTEASOMAL UBIQUITIN RECEPTOR ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04683">
        <property type="entry name" value="Rpn13_ADRM1_Pru"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51917">
        <property type="entry name" value="PRU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pru" evidence="7">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="133"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="298" mass="33137" checksum="519DA7552A81EF81" modified="2011-11-16" version="1">MPSSKSVKFHAGKVQYDDETNRCTPLPHKGVISIKPSVDEPDFLDFTWSAKSDSTQSSVGTIEKDEFLLIPGDVTIKKIQSCNTGRVIALTFLSSGAKYLYWLQDVGDIDQLDKWTDKDDKIIRDISELIYVNDEDEEEEDEEAETPNEEVEVKDEGSEQQPVATAATAATSAKPDLKLPISSISSVLDLETIDKHLAGLNTEQLKELYGQFLPQSVAENPTKEQILDVIRSGFFQQSEQKLSQYLTQGNGAGYLLAQSLKFDYHGEGIDGFLTGIRELAKKEKSDEKAEGEDTEMKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFQ6</accession>
    <name>G3AFQ6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein SYG1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_64237</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYG1 (TC 2.A.94) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372457.1">
        <property type="nucleotide sequence ID" value="XM_007372395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFQ6"/>
    <dbReference type="FunCoup" id="G3AFQ6">
        <property type="interactions" value="539"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFQ6"/>
    <dbReference type="GeneID" id="18874965"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64237"/>
    <dbReference type="eggNOG" id="KOG1162">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006116_1_1_1"/>
    <dbReference type="InParanoid" id="G3AFQ6"/>
    <dbReference type="OMA" id="GDMYCSL"/>
    <dbReference type="OrthoDB" id="9970435at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14475">
        <property type="entry name" value="SPX_SYG1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF103">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 53 MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="423"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="461"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="507"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="541"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="694"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="737"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="9">
        <location>
            <begin position="1"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="8">
        <location>
            <begin position="626"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="40"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="258"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="845"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="906"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="845"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="916"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="931"/>
            <end position="941"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="948" mass="110388" checksum="204A310BAD683DE4" modified="2011-11-16" version="1">MKFGESLSEGLVPEWHDQYVDYKAGKKLIKKLVSLREEQQSGYNTDDSKKAAQDTTPLLEAQEERNRYTGVEDIITDEPLSPNRNFTSSDGEDLTEFPTRPKLKVRARSVFGFDSFNLGRDKSEDYTHISEKFNSWLESELVKVNTFFSEKEQDVYERFLLLQDQLYQLRDHKLSIIKERTQQQRGPSPKISNKFPFQNRLSLQELNRLELPSLPSGTFLKKLGRRRNESTGDDISLHTDDSVDVNYAENRIRNGLTDLGADDQSLGSDVEVDIPDRPRPPETAEQARQSQRRDYVVKKQHFGVPYLFARRQLKDAILEHYRSLALLRSYKTLNRTAFRKITKKYDKLLKKDTMKAFMNRIDNHAYFLTSDLVDKLINQVEESYIAFFDPETKDRKHSLEKLKSIAYALNASEMKPPNFYSEFFTSGFALGFGIPLLGTAIYVAIHNIITGLLPEGKYLLQIWAGFFLLMLMFILFGVNLAVFDKYKINYKFIFEFNMSTVMNYKQFWLLPSLGFALLCILTWFSVHDFWPSAFAGRDWPWLYLGIIVLVFIWPGNQFYSSSRRWLQVALWRLLLSGFYPVEFRDFFLGDIVCSLTYTMGNISFFFCIYAHHWNGALSGNPGEDNVCGSGKSRLMGFCSTLPSIWRFLQCVRRYMDTGDWFPHLANMMKYTMSALYQITLSMYRIERNNANKSTFILFACINSLYTSAWDIFMDWSLMQSGSKNFLLRDHLFYKRPIYYYSAMIVDVILRFQWIFYAFFSHQIQQSAVTSFCIAVAEIIRRFIWIFFRMENEHCSNVILFRASKDTPLPYSISPKVQRAIKKLVMLKYTTEPLLVEQVSTQPQETVGVTQTPYSTGSLARDEEEIDSDEVSQIRLDTVRSALPQLTRRKSTFQTISESLHTAHIKDFQRRKVATQADDESDDEDEEESLHAAHRTSSRHSNRSSGRDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFR4</accession>
    <name>G3AFR4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Histone acetyltransferase type B catalytic subunit</fullName>
            <ecNumber evidence="4 14">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">SPAPADRAFT_58187</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalytic component of the histone acetylase B (HAT-B) complex. Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. Involved in DNA double-strand break repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the HAT-B complex composed of at least HAT1 and HAT2. The HAT-B complex binds to histone H4 tail.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the HAT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="4 14"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372465.1">
        <property type="nucleotide sequence ID" value="XM_007372403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFR4"/>
    <dbReference type="FunCoup" id="G3AFR4">
        <property type="interactions" value="1162"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFR4"/>
    <dbReference type="GeneID" id="18872362"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58187"/>
    <dbReference type="eggNOG" id="KOG2696">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036024_2_1_1"/>
    <dbReference type="InParanoid" id="G3AFR4"/>
    <dbReference type="OMA" id="WTCDAND"/>
    <dbReference type="OrthoDB" id="10253098at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000123">
        <property type="term" value="C:histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043997">
        <property type="term" value="F:histone H4K12 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043995">
        <property type="term" value="F:histone H4K5 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043996">
        <property type="term" value="F:histone H4K8 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000114">
        <property type="entry name" value="Histone acetyltransferase type B catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.360.10">
        <property type="entry name" value="Histone acetyl transferase 1 (HAT1), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019467">
        <property type="entry name" value="Hat1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037113">
        <property type="entry name" value="Hat1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017380">
        <property type="entry name" value="Hist_AcTrfase_B-typ_cat-su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013523">
        <property type="entry name" value="Hist_AcTrfase_HAT1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12046">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE TYPE B CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21184">
        <property type="entry name" value="HAT1_C_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10394">
        <property type="entry name" value="Hat1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038084">
        <property type="entry name" value="HAT-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14 20" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histone acetyl transferase HAT1 N-terminal" evidence="19">
        <location>
            <begin position="15"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="18">
        <location>
            <begin position="180"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with histone H4 N-terminus" evidence="16">
        <location>
            <begin position="47"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with histone H4 N-terminus" evidence="16">
        <location>
            <begin position="197"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="15">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="222"/>
            <end position="224"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with histone H4 N-terminus" evidence="17">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR038084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF10394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EGW35053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="398" mass="45906" checksum="5E6B4F06372ABD2F" modified="2011-11-16" version="1">MSTSISAASLQPEQWTTSANEAVKLFVTDATGAINFSPTFTYPIFGDAETIYGYKDLVIFLCFDHFTFKPFFNVKYSAKLDDPDIVDLEQVLRKFLPESAIYKDESKWADSIQEEKEAGYTIPGELIDSFEKDDSAYEIYKINVKDASGLELHKRLQILVLLFIEAGSYIDTSDELWDLYVLYNKDSSSIVGFATAYNYWKYPGSVKFDDNKREIRKKISQFIILPIYQGKGLGGALYSHLFDVWHKDEEVVEVVVEDPNESFDDMRDRADLSRLNKVLEFSKVTPKVESSWVEETRKSLKLEKRQFSRLLELILLYKLKHSNEVTKKDVRLFIKKRLYNKNKEGLSALDENTKKDKLQTAYEALEDDYYRILGDLRLSIKRAGDNQNNGNSKKAKVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFT7</accession>
    <name>G3AFT7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">HRDC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_53425</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the exosome component 10/RRP6 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372488.1">
        <property type="nucleotide sequence ID" value="XM_007372426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFT7"/>
    <dbReference type="FunCoup" id="G3AFT7">
        <property type="interactions" value="1012"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFT7"/>
    <dbReference type="GeneID" id="18871784"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53425"/>
    <dbReference type="eggNOG" id="KOG2206">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010129_3_2_1"/>
    <dbReference type="InParanoid" id="G3AFT7"/>
    <dbReference type="OMA" id="LEYKFLH"/>
    <dbReference type="OrthoDB" id="2250022at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071044">
        <property type="term" value="P:histone mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071040">
        <property type="term" value="P:nuclear polyadenylation-dependent antisense transcript catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071042">
        <property type="term" value="P:nuclear polyadenylation-dependent mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071036">
        <property type="term" value="P:nuclear polyadenylation-dependent snoRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071037">
        <property type="term" value="P:nuclear polyadenylation-dependent snRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032204">
        <property type="term" value="P:regulation of telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06147">
        <property type="entry name" value="Rrp6p_like_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000059">
        <property type="entry name" value="Exosome complex exonuclease Rrp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012588">
        <property type="entry name" value="Exosome-assoc_fac_Rrp6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002121">
        <property type="entry name" value="HRDC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045092">
        <property type="entry name" value="Rrp6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049559">
        <property type="entry name" value="Rrp6p-like_exo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12124:SF47">
        <property type="entry name" value="EXOSOME COMPONENT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12124">
        <property type="entry name" value="POLYMYOSITIS/SCLERODERMA AUTOANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00570">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08066">
        <property type="entry name" value="PMC2NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00341">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50967">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0271">Exosome</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="HRDC" evidence="10">
        <location>
            <begin position="451"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="681"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="723"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="742"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW35076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="756" mass="86820" checksum="E41865CE57BE1A1B" modified="2011-11-16" version="1">MSSEMEDVFKGVLPQLVSTIRAASSLAAQDINFYKSIDAGLASDIDSAAARLMNISNDLIRATTDDDEVKIGFGKQNVEGELSWNPIARVLDSIFEKIDYSFDHARQKTSSDARSLEYLDSGSAPSNESGKRISKPQLKFRVPVDNSESHPFKPRLDSKPHALKSFEESVKLFNPEPKYEDSIEIIDPPYYPQPYEYEIDNQPYPEAILQSAVEPIPSKDWSTTEAIWVDTVEELTKMVTVLQSSTEIAVDLEHHDYRTYYGIVCLMQISNRDQDWIIDTLALRDDLTVLNTVFTDPSIVKVFHGAFMDIIWLQRDLGLYIVSLFDTYCASKNLGFPKHSLAYLLETFAHFKTSKKYQLADWRIRPLSHPMMAYARSDTHFLLNIYDQLRNKLIHENKLQQVLYDSRQVAKRRFEYTKYRPLAPNGKVSCPVMSSNPREPFASIMYQYNVPSFKKGVVEVLYNWRDLVAKQEDESVRYIMPNQLLVTLALLESPVDANKVLNCQTYVSEHVRLHAKELAGLIEKTLVQTEANDWDLVDKWSNQKTDSFDAHDKNMLENVNGLFNALVESNQDLFNSTGLVHEIETVEEPKIRYTVEYDVNNNTVVKHNFEDVCQERLERAWNGLIAMDAQSNVEVPIEEVPETEEPEAPVEFVKPARPDAALFSKDKEIDPNQLITLRQRKVAQPKRRQLLEEQEQQQVDYSSADKIMIPNTNRRPKDRKKKSFDPYSRESEGPKPAKRAKITTTGKSSTFKNKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFW7</accession>
    <name>G3AFW7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Clathrin light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_58247</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasmic vesicle membrane</location>
            <topology evidence="1 6">Peripheral membrane protein</topology>
            <orientation evidence="1 6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Coated pit</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="6">Cytoplasmic face of coated pits and vesicles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the clathrin light chain family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372518.1">
        <property type="nucleotide sequence ID" value="XM_007372456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFW7"/>
    <dbReference type="FunCoup" id="G3AFW7">
        <property type="interactions" value="352"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFW7"/>
    <dbReference type="GeneID" id="18872385"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58247"/>
    <dbReference type="eggNOG" id="KOG4031">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069856_0_1_1"/>
    <dbReference type="InParanoid" id="G3AFW7"/>
    <dbReference type="OMA" id="FYENYNT"/>
    <dbReference type="OrthoDB" id="5512at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030130">
        <property type="term" value="C:clathrin coat of trans-Golgi network vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000996">
        <property type="entry name" value="Clathrin_L-chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10639">
        <property type="entry name" value="CLATHRIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10639:SF7">
        <property type="entry name" value="CLATHRIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01086">
        <property type="entry name" value="Clathrin_lg_ch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0168">Coated pit</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="106"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="221" mass="25548" checksum="9BC30A43D1CA2376" modified="2011-11-16" version="1">MADKFPEIETIGEDLPQDEQLDDSDFLTRERQLVGDQFTTEQDKDVWADEDDDINEFKEQFPEVDDSAPVQVPEVAQDEEEEDEFEAFGGAAAPASDKYQGESQALKEWKERRDLEISEREKANAKTKEDIISKAKQTIDDFYDNYNLKREEHSKQVLKEQEQYLEKRDGFLKRGTLWDRVNELVEEVGELPENEDRDKSRFKGLLTKLKGKESAPGAGGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFX8</accession>
    <name>G3AFX8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58281</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372529.1">
        <property type="nucleotide sequence ID" value="XM_007372467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFX8"/>
    <dbReference type="GeneID" id="18872392"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58281"/>
    <dbReference type="HOGENOM" id="CLU_1455246_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFX8"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="134"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="186" mass="18897" checksum="E519BFD9F111920C" modified="2011-11-16" version="1">MPGNHMLIAREESDMMSQMTGMVPTMTSGMMPGLETSGGDSMMMSDTMMKSDMTSDMMSDMMSDTMMKSDMMSESMTMSDTVVMSNPMMKSDMSSMGGNGTRIGGGPPGGPGRTATRSLPGAGSGTGDDDMMMTSSPDGDSSASDSSESGGATTSTGNGAGAVAANWKKSAGVASIVGLSFILSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFY2</accession>
    <name>G3AFY2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58285</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372533.1">
        <property type="nucleotide sequence ID" value="XM_007372471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFY2"/>
    <dbReference type="STRING" id="619300.G3AFY2"/>
    <dbReference type="GeneID" id="18872393"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58285"/>
    <dbReference type="eggNOG" id="ENOG502SCRY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175850_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFY2"/>
    <dbReference type="OMA" id="SHIPSAM"/>
    <dbReference type="OrthoDB" id="88410at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34724">
        <property type="entry name" value="OS12G0596101 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34724:SF2">
        <property type="entry name" value="OS12G0596101 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="81" mass="8904" checksum="FDEBA3E9D088743B" modified="2011-11-16" version="1">MCNAAICAICNKKSWTGCGQHIPEIMDRTPKESWCTCEPNDGAEKVTVEGHGSYPPRATTGCRRGSTTYVPILRDYSVESH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG22</accession>
    <name>G3AG22_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RNA exonuclease 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_58364</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">3' to 5' exoribonuclease required for proper 3' end maturation of MRP RNA and of the U5L snRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the REXO1/REXO3 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372572.1">
        <property type="nucleotide sequence ID" value="XM_007372510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG22"/>
    <dbReference type="STRING" id="619300.G3AG22"/>
    <dbReference type="GeneID" id="18872415"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58364"/>
    <dbReference type="eggNOG" id="KOG2248">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008679_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG22"/>
    <dbReference type="OMA" id="HELAMIK"/>
    <dbReference type="OrthoDB" id="206335at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06145">
        <property type="entry name" value="REX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000031">
        <property type="entry name" value="RNA exonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034922">
        <property type="entry name" value="REX1-like_exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF115">
        <property type="entry name" value="FI18136P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="9">
        <location>
            <begin position="31"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="397" mass="46434" checksum="4180238FF76125D6" modified="2011-11-16" version="1">MSERDMISHNYPMFVKDDTWRETIARDDESHIYAIDCEFCKAGTQQVLTRASLIDFEGNVVFDEFVKPAQEITDYVTRYSGITEEILRDVTTTLEQVQQLFIDKISANDILVGHSLESDLEVLKIKHNRVVDTAIVYDHNRGPPAKPSLRWLAQKYLDQKIQSGEDTGEGHSSVEDAKASLDLVKMKIVEGKCFGVNVNEVSIFKRLASNYNLTKKTGNSDEEETDFRSLWINYSHYKDQETYVEPEDYQLDRVYVNNDDEVVDKFKSEVQGKRFVVLQLREMEFNNKWCTAPQHYDGRLDYQQSELYQRTNDRLQSIYENLPENSLFIVYSQSRSPLEMYRLQEIRQKFQKLERDGVDVTKLPQEESWDFDKQQELIEKTAIARESLAFVKLKQTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGD2</accession>
    <name>G3AGD2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">AP endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_132284</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="7">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372683.1">
        <property type="nucleotide sequence ID" value="XM_007372621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGD2"/>
    <dbReference type="FunCoup" id="G3AGD2">
        <property type="interactions" value="491"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGD2"/>
    <dbReference type="GeneID" id="18869649"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132284"/>
    <dbReference type="eggNOG" id="KOG1294">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010374_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGD2"/>
    <dbReference type="OMA" id="YTVWNTL"/>
    <dbReference type="OrthoDB" id="391817at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020848">
        <property type="entry name" value="AP_endonuclease_F1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF4">
        <property type="entry name" value="DNA-(APURINIC OR APYRIMIDINIC SITE) ENDONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00728">
        <property type="entry name" value="AP_NUCLEASE_F1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="10">
        <location>
            <begin position="27"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="428"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="428"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="6">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="371"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="8">
        <location>
            <position position="344"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA substrate" evidence="8">
        <location>
            <position position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW35271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="484" mass="54988" checksum="093BA837DCAB89DA" modified="2011-11-16" version="1">MNIKDLQTQDPLNKIHRKKSSQSVRFVTFNVNGAKTLFNYHPWTKFNNDLNSVFTSLQADVITLQELKVSPSNLSTIKDVGHLQNYKSFISLPRVKKGYSGVGVFVRIPQENESNAVKRSLTVVKAEEGITGWLCPRNSKLSYRTMDKEQQIGGYVDDLDSQQGLNLDSEGRCVVIEFTNNLVVFSLYCPANSSGTEEGEEFRLKFMKVLFERCYNLKFKLGKEVLIMGDINISTDLIDHAQTIEDMVLNRLGKNSPNGNNFETINYEQCCDFKKSTPARHLLNEYVIPGLWHDLSLQSSPSDSHRKQFLYDTTRFLQGRRMKMYTVWNTLTNSRAVNFGSRIDLILTSSYHMVKSVSQADIWEFILGSDHCPVFTDFEAGYDDLKQEEEVVLPCVTLPFEAKLHYKLTKNRDISSLFAPRKSKSTDSVEVTPLSDSANSQSSTQAEDSASEQPAKRPKLNYVSRKVDKSQKKGNQKSISSFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGF0</accession>
    <name>G3AGF0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_146373</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372701.1">
        <property type="nucleotide sequence ID" value="XM_007372639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGF0"/>
    <dbReference type="FunCoup" id="G3AGF0">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGF0"/>
    <dbReference type="GeneID" id="18870713"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_146373"/>
    <dbReference type="eggNOG" id="ENOG502S83P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152072_2_0_1"/>
    <dbReference type="InParanoid" id="G3AGF0"/>
    <dbReference type="OMA" id="VFTEGWP"/>
    <dbReference type="OrthoDB" id="2391627at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019182">
        <property type="entry name" value="Cytochrome_b-c1_su10_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029027">
        <property type="entry name" value="Single_a-helix_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28254">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28254:SF1">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09796">
        <property type="entry name" value="QCR10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81518">
        <property type="entry name" value="Subunit XI (6.4 kDa protein) of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="92" mass="10346" checksum="2829324526347AC7" modified="2011-11-16" version="1" fragment="single">SAFLLLSQHRHIKQNGFNVRGPVYKTVQNFGKFNINTVRSYVPNLILWGGAAGAAVAVFTEGVPLFRNTFYSKIPYFGEHFVYNPDPEDVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGN8</accession>
    <name>G3AGN8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbohydrate kinase FGGY C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_58599</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372789.1">
        <property type="nucleotide sequence ID" value="XM_007372727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGN8"/>
    <dbReference type="FunCoup" id="G3AGN8">
        <property type="interactions" value="291"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGN8"/>
    <dbReference type="GeneID" id="18872525"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58599"/>
    <dbReference type="eggNOG" id="KOG2517">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009281_10_1_1"/>
    <dbReference type="InParanoid" id="G3AGN8"/>
    <dbReference type="OMA" id="ITEMAHA"/>
    <dbReference type="OrthoDB" id="203824at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019150">
        <property type="term" value="F:D-ribulokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019321">
        <property type="term" value="P:pentose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43435:SF4">
        <property type="entry name" value="FGGY CARBOHYDRATE KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43435">
        <property type="entry name" value="RIBULOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="4">
        <location>
            <begin position="88"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW35377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="498" mass="54451" checksum="E957EB700EA3C3A0" modified="2011-11-16" version="1" fragment="single">MDELIEDNFRRLGGIPGKNGRYLSAGDIVGKLNAEAAEELGLTTECIVGSPVIDAYAGWVGTVAAQADITKLQQTEVPGKIITACGRLAAVAGTSTCHIAMTKEPCFVQGVWGPYKDVMAEGYWLAEGGQSCTGQLLAHVLSIHPASQELSRAAEASNLSKFDYLNLTLENLVLERNERSVVSLAKHMFFYGDFHGNRSPIADPRMKANIIGQSMDSSVKDLAIQYFAACEFIAQQTRQIVEEMEKSGHDISCIFMSGGQCRNGLLMRLLADCTGLPIVIPRYIDAAVVFGAALLGAAAAEDSVKEYSLTKGRSRRESTLADKKKSQVSLHTYDDIASPYTQPSATSTTNLATLAYAGGSHSHLAQEVDYFNQPTAGSPLAKVSSSAIHDSENEESDDETLSFGSKHKVQQGLSQELHKMKLTKLTSINEQKAEDSTHADKLWKIMEKMTGPGKVIYPSEDNDPDRKLLATKYKVFLDQCFKQQEYRRMVDEIEKENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGP6</accession>
    <name>G3AGP6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Agglutinin-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_146555</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ALS family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372791.1">
        <property type="nucleotide sequence ID" value="XM_007372729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGP6"/>
    <dbReference type="FunCoup" id="G3AGP6">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="GeneID" id="18870720"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_146555"/>
    <dbReference type="eggNOG" id="ENOG502S1H2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031316_2_0_1"/>
    <dbReference type="InParanoid" id="G3AGP6"/>
    <dbReference type="OMA" id="WHINSSM"/>
    <dbReference type="OrthoDB" id="3981162at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044403">
        <property type="term" value="P:biological process involved in symbiotic interaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2430">
        <property type="entry name" value="Agglutinin-like protein, N-terminal domain, N2 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008966">
        <property type="entry name" value="Adhesion_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008440">
        <property type="entry name" value="Agglutinin-like_ALS_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024672">
        <property type="entry name" value="Agglutinin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043063">
        <property type="entry name" value="Agglutinin-like_N_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033504">
        <property type="entry name" value="ALS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011252">
        <property type="entry name" value="Fibrogen-bd_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793:SF2">
        <property type="entry name" value="AGGLUTININ-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793">
        <property type="entry name" value="ALPHA-AGGLUTININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05792">
        <property type="entry name" value="Candida_ALS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11766">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01056">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49401">
        <property type="entry name" value="Bacterial adhesins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0134">Cell wall</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 17" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0843">Virulence</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442306" description="Agglutinin-like protein N-terminal domain-containing protein" evidence="17">
        <location>
            <begin position="18"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="Agglutinin-like protein N-terminal" evidence="18">
        <location>
            <begin position="53"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="440"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="19">
        <location>
            <position position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW35379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="549" mass="59595" checksum="189237C58A484D2F" modified="2011-11-16" version="1" fragment="single">MFLSLIFLQILLTFAAAKEISGVFTSFNSLKWRKGANYRYASPAFPSWIAELGWKIDGSKMSAGDTFTLTMPCVFKFTTTQVSVNLKVGNTIYATCRFAPGDILLPTSELKCSLSDTVKQSTSAHGTMSFPLTFNVGGSALDTDVKCGETFTSGVNTVSFSDGDNVVSTTVNFEGGVDTNPDRIAYNARVVPSVNKLQHQVLGGKCGAGYSSGVLGITMLYGTGSIDCSSIHAAISNSLNDWYFPKSIETDFSYTTKCSATSFIINYKNIPAGYRVFIDTLVSSAVGVKHKIRYTNRYKCANSPYIIDNSETVQWQPYCNDVAGANGKEVELVTETWTGSTTLVYTKPFKTHDPTITIVVDVPIPTITYTTTYTGISTSYTTYTVTPGETASVVEYEPVHATTSEVSCWETDSTATTTIIVSTRATDTVLVITPCPKTKVETTTKQEQPITTDKPEPTTVEQVTSEEPEPTTVEQVTSEEPEPTTVEQVTSKEPEPTTVEQVTSEEPEPTTVEQVTSKEPEPTTVEQVTPEEPESTEVIVTTDCNTPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGQ5</accession>
    <name>G3AGQ5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CoA-transferase family III</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_146575</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CoA-transferase III family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372800.1">
        <property type="nucleotide sequence ID" value="XM_007372738.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGQ5"/>
    <dbReference type="STRING" id="619300.G3AGQ5"/>
    <dbReference type="GeneID" id="18870721"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_146575"/>
    <dbReference type="eggNOG" id="KOG3957">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021588_1_1_1"/>
    <dbReference type="InParanoid" id="G3AGQ5"/>
    <dbReference type="OMA" id="GEFFHIH"/>
    <dbReference type="OrthoDB" id="5863171at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="entry name" value="Crotonobetainyl-coa:carnitine coa-transferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052985">
        <property type="entry name" value="CoA-trans_III_biosynth/detox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48229:SF1">
        <property type="entry name" value="ALPHA METHYLACYL-COA RACEMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48229">
        <property type="entry name" value="CAIB/BAIF FAMILY ENZYME (AFU_ORTHOLOGUE AFUA_1G05360)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="CoA-transferase family III (CaiB/BaiF)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="548" mass="61137" checksum="7DE7E2656E9C656B" modified="2011-11-16" version="1">MTVESYSPISETQRIFQTLIESPLIPSELKQYAQSIEFHNDRAELSVPCPLKQTEMISALKALEATVALGLAHSRFTEVDNACKIDLQHALLFLMSTYCASVDGYFKGDKKEVLKYLKPTDLNQAQSDPYRRMSANLYRTKDGRYYHIHGSLEANTTLEMIGLPTFIKGMTDYDEIVKIYQDKLDTFNCDELEALNVKNKQAGIEALKPEQFLETPHGKTISNTPEFEVDALETSTPPVPFAPTSSKKLLEGIKVLELCRIIAGPTIGKILAEYGATVIKVTADDTLPDVPFFQVDGNMGKHTTNLNLKNAEDRVKFEALLKDADVVIDGYRAGAIEKLGYGPKKLAELAQSRNKGYIYGSENCFGFTGEWSGRAGWQQIADCASGVAWVQGTSMGNNQPMVPPFPMSDYGTGCMVAIAILVAIYKRNKFGGSYWAKSSLVQYDLLLLKQGTYPDQIWQSQVLENPIFADFLKLHYYDSVDKISNTALKALLKQQPDILNNGHYFTSMESEGFKGTIRVLRPVCEFNSLELGYKCVSRPNGSDAPEWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGZ8</accession>
    <name>G3AGZ8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">40S ribosomal protein S4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_47759</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the eukaryotic ribosomal protein eS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372083.1">
        <property type="nucleotide sequence ID" value="XM_007372021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGZ8"/>
    <dbReference type="FunCoup" id="G3AGZ8">
        <property type="interactions" value="1252"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGZ8"/>
    <dbReference type="GeneID" id="18871142"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_47759"/>
    <dbReference type="eggNOG" id="KOG0378">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060400_1_0_1"/>
    <dbReference type="InParanoid" id="G3AGZ8"/>
    <dbReference type="OMA" id="KANDTHK"/>
    <dbReference type="OrthoDB" id="1109245at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06087">
        <property type="entry name" value="KOW_RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000005">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.740:FF:000001">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00485">
        <property type="entry name" value="Ribosomal_eS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000876">
        <property type="entry name" value="Ribosomal_eS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032277">
        <property type="entry name" value="Ribosomal_eS4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013845">
        <property type="entry name" value="Ribosomal_eS4_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038237">
        <property type="entry name" value="Ribosomal_eS4_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041982">
        <property type="entry name" value="Ribosomal_eS4_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013843">
        <property type="entry name" value="Ribosomal_eS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018199">
        <property type="entry name" value="Ribosomal_eS4_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16121">
        <property type="entry name" value="40S_S4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00900">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08071">
        <property type="entry name" value="RS4NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002116">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00528">
        <property type="entry name" value="RIBOSOMAL_S4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 12" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7 8" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein eS4 N-terminal" evidence="10">
        <location>
            <begin position="3"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Small ribosomal subunit protein eS4 central region" evidence="9">
        <location>
            <begin position="95"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Small ribosomal subunit protein eS4 C-terminal" evidence="11">
        <location>
            <begin position="212"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR002116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW34671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="262" mass="29340" checksum="7D1C9D2DC38C0A2D" modified="2011-11-16" version="1">MARGPKKHLKRLAAPSHWMLDKLSGTYAPRPSAGPHKLRESLPLVVFLRNRLKYALNGREVKAILMQQHVKVDAKVRTDSTFPTGFMDVITLEATNEHFRLIYDVKGRFAVHRISAEEAAYKLGKVKKVQLGKKGIPYVVTHDGRTIRYPDPLIRVNDTVKIDLATGKISDFIPFDTGRLVMVTGGRNLGRVGAIVHKEHHAGGFDLVHIKDSLDNTFVTRLSNVFVIGTEAGKPWVSLPKGKGIKLTISEERDRRRTQQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH05</accession>
    <name>G3AH05_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Holocytochrome c-type synthase</fullName>
            <ecNumber evidence="10">4.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_58655</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Lyase that catalyzes the covalent linking of the heme group to the cytochrome C apoprotein to produce the mature functional cytochrome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>holo-[cytochrome c] = apo-[cytochrome c] + heme b</text>
            <dbReference type="Rhea" id="RHEA:22648"/>
            <dbReference type="Rhea" id="RHEA-COMP:10725"/>
            <dbReference type="Rhea" id="RHEA-COMP:10726"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:83739"/>
            <dbReference type="EC" id="4.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cytochrome c-type heme lyase family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.17" evidence="10"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372847.1">
        <property type="nucleotide sequence ID" value="XM_007372785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH05"/>
    <dbReference type="FunCoup" id="G3AH05">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AH05"/>
    <dbReference type="GeneID" id="18872556"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58655"/>
    <dbReference type="eggNOG" id="KOG3996">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048602_1_2_1"/>
    <dbReference type="InParanoid" id="G3AH05"/>
    <dbReference type="OMA" id="SCPVDHK"/>
    <dbReference type="OrthoDB" id="4243at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004408">
        <property type="term" value="F:holocytochrome-c synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000511">
        <property type="entry name" value="Holocyt_c/c1_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12743">
        <property type="entry name" value="CYTOCHROME C1 HEME LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12743:SF0">
        <property type="entry name" value="HOLOCYTOCHROME C-TYPE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01265">
        <property type="entry name" value="Cyto_heme_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00821">
        <property type="entry name" value="CYTO_HEME_LYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00822">
        <property type="entry name" value="CYTO_HEME_LYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0349">Heme</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW35435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="264" mass="29498" checksum="899115F8DEC3986D" modified="2011-11-16" version="1">MADESEQPKCPVDPSTRSSWLSLWKKPAPQVDIPKQKQEPEAAAAAAAAGDQPKCPVDHNARSAWMNNVSVQINTEAIEEPEGCSSNAVNQDLTTAGAPSDLATEREISSIPRTSADSNWIYPSQKQFFDAMKRKQWNPEKEDMKTVVPIHNAVNEQAWRHIQMWEAPYAEETHQKCGGVSLTSFKGDSKKLTPRAWFKSSVLGYAKPFDRHDWTVDRCGVSVEYVIDFYGSEGTTGPSFFLDVRPKINTLEGFKLRLRRALGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH37</accession>
    <name>G3AH37_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ferric-chelate reductase (NADPH)</fullName>
            <ecNumber evidence="3">1.16.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_146752</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2 a Fe(II)-siderophore + NADP(+) + H(+) = 2 a Fe(III)-siderophore + NADPH</text>
            <dbReference type="Rhea" id="RHEA:28795"/>
            <dbReference type="Rhea" id="RHEA-COMP:11342"/>
            <dbReference type="Rhea" id="RHEA-COMP:11344"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.9" evidence="3"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372879.1">
        <property type="nucleotide sequence ID" value="XM_007372817.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH37"/>
    <dbReference type="FunCoup" id="G3AH37">
        <property type="interactions" value="394"/>
    </dbReference>
    <dbReference type="GeneID" id="18870725"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_146752"/>
    <dbReference type="eggNOG" id="KOG0039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010365_4_0_1"/>
    <dbReference type="InParanoid" id="G3AH37"/>
    <dbReference type="OMA" id="FITYHRH"/>
    <dbReference type="OrthoDB" id="167398at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF9">
        <property type="entry name" value="FERRIC REDUCTASE TRANSMEMBRANE COMPONENT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="7 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442374" description="ferric-chelate reductase (NADPH)" evidence="17">
        <location>
            <begin position="20"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="351"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="18">
        <location>
            <begin position="414"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW35467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="719" mass="83317" checksum="78A2572CBA99C026" modified="2011-11-16" version="1">MNIFSLLYFGFILLAGVQAHGKEHFTWYKKSLAIYACNYEISYLKGISFCPAKGNKRERKKCRCANENWRASLAGCLAYKERNTTSTIEFYKSFCQNNYNVDLDKDWFDKSYTHFLNNAKNKYEIKPSREPVNFPVKFSPKEMDLYGKVAINFLGNYDDSIWYGISMFGFWFLVLTIGAISHWTKVLFPETTKKLVGSISNSYRKYISIPALYGKRRAQEIRCWKVFDSLIPTRFESIVIGLFYLFTVIIHAVNLKAVENDPIFDTKYMAEIRYVADRTGIVGTAMTPLVFLFAGRNNFLQWITGINFSTFLCYHRHIARVMVMLVVIHSVNYTILLVYRETFPFKVKNPWLYWGILATVAGGLALFQSILYLRRSHYELFLFLHLALAVLFVAGTWIHIVDFGYCVLLYPCIAVWCFDRIIRLIRLFSFGFPQANVMLVGDETIKLEIPKPKYWKPVPGGHVFVHFLKPSYFWQSHPFTFVKSSEKEGIIVIYLKLKGGITQNLYKLLKKSPGKMATMRVGIEGPYGESTPAKYSDKAVFIAGGNGIPGIYSEVVDISLRSPSERKSQLKLIWVIRDYNSIIWFYDELLWLKNTDIKTTLFVTRPNNRVTNFGSFNKSESSQEGSIKSGYNEINPQPYKGQNYIEEFKAELSHINFEERRPNFEQLIHDEIEESPGSVAFVACGHPAMVDEVRYQSCRNIDNPYNKRVDFYEQLQVWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHA9</accession>
    <name>G3AHA9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MICOS complex subunit MIC10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_58771</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Mitochondrion inner membrane</location>
            <topology evidence="2 9">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the MICOS complex subunit Mic10 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372951.1">
        <property type="nucleotide sequence ID" value="XM_007372889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHA9"/>
    <dbReference type="FunCoup" id="G3AHA9">
        <property type="interactions" value="92"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHA9"/>
    <dbReference type="GeneID" id="18872598"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58771"/>
    <dbReference type="eggNOG" id="KOG4853">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068905_3_0_1"/>
    <dbReference type="InParanoid" id="G3AHA9"/>
    <dbReference type="OMA" id="SNCRHDL"/>
    <dbReference type="OrthoDB" id="1916310at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007512">
        <property type="entry name" value="Mic10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21304">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21304:SF0">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04418">
        <property type="entry name" value="DUF543"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="84" mass="9133" checksum="3B395D82A1CE4FAC" modified="2011-11-16" version="1">MSEQQQKQIVTPSQNLLNEKWDVVLSNALIKTGLGFGGGVLASILLFKRRSFPVWLGIGFGLGRGYSEGDAIFRSAHGLRTVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHJ0</accession>
    <name>G3AHJ0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dit2 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_54339</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373738.1">
        <property type="nucleotide sequence ID" value="XM_007373676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHJ0"/>
    <dbReference type="FunCoup" id="G3AHJ0">
        <property type="interactions" value="1520"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHJ0"/>
    <dbReference type="GeneID" id="18871855"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54339"/>
    <dbReference type="eggNOG" id="KOG0157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031576_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHJ0"/>
    <dbReference type="OMA" id="FFGSRWN"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11070">
        <property type="entry name" value="CYP56-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000106">
        <property type="entry name" value="Cytochrome P450-DIT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF223">
        <property type="entry name" value="CYTOCHROME P450-DIT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="470" mass="53782" checksum="9D51CB3E33AF2F50" modified="2011-11-16" version="1">MLSEILKYLVIAIGSYSVYLVLEIIFPPFNFPKTIPTIPFYVSFLGSYENLDQEQIYKRYLKDKLEKYGAVKIYFASRWNILVSRPEYLLTIFKNEDVYAKSGNHIKIPGSVMASYTGDNIISAHGELWKLYREVVAPSIQFPDKEPVYKNTKMMLEIIENELKNSDTIAIADILQRYTLNNVGNSMIGIDFRALEGSQSFMHQKIKYVKSQIFRPFYMNFPYFDKFPIPSRLTAKREVNSFRNWYGQKLIDEHKPELSNSAATKLVNALKDGKLTEKQYLDNAVIVMVAGHENPLLLMLSVLFILGKNPDLQESLRTEKIPLESVIYETLRVYPPLAQIINRCTTKATVLGENIFIPKGVYVGYNNLATGRDRGIWGPDADEFNPSRWGNTLDEINQNYSQAKRMATLPAFHGRKRACLGEKFAIFEIKECISAIIGKYRISLGENWKPKLTPSGPVCPLGLQIKFEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHL5</accession>
    <name>G3AHL5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59612</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373763.1">
        <property type="nucleotide sequence ID" value="XM_007373701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHL5"/>
    <dbReference type="GeneID" id="18872986"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59612"/>
    <dbReference type="eggNOG" id="ENOG502QRYW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_776518_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHL5"/>
    <dbReference type="OMA" id="QQNKYAL"/>
    <dbReference type="OrthoDB" id="4026807at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="334"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="166"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="192"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="334"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="357" mass="38848" checksum="DBD8E7354870B0B3" modified="2011-11-16" version="1">MSTTTSYPKRKLELIPFNNQIEYIDKVIVNNSKHQNQFSNYGSRYNHGHGGHGHNHHNQQQNKYALAAVAVAAAVQHQQQQHYLLQQQQQVRQGQQQKQGYYSGGYQQQQQQQQYNPPQYQKQPSQTSSPQMYGLQQQPQPQQYQPPQIFADVGPSVLPEANNSFTQGQQTTGGSSSQAPGYDLKTNFMIYNNSSNSISSPTSQQPQLKQSSVSSASSATATLPGGPLLPKLFDSIGTNSTNNSPILLPPNANTYKNSSPPDLFLSNTTGNSTSTLNNSPPLVNTPTPTTNFFSSPSLLPGSSSNWNTSNPSTSSIWGQSSNSFKSNITLANPTTSTTATGNGKNNLLSSFGSTNLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHN5</accession>
    <name>G3AHN5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Glutamine synthetase</fullName>
            <ecNumber evidence="3 11">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">GLN1</name>
        <name evidence="14" type="ORF">SPAPADRAFT_49262</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 11">
            <text>L-glutamate + NH4(+) + ATP = L-glutamine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9 10">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="3 11"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373783.1">
        <property type="nucleotide sequence ID" value="XM_007373721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHN5"/>
    <dbReference type="FunCoup" id="G3AHN5">
        <property type="interactions" value="789"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHN5"/>
    <dbReference type="GeneID" id="18871322"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49262"/>
    <dbReference type="eggNOG" id="KOG0683">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036762_1_1_1"/>
    <dbReference type="InParanoid" id="G3AHN5"/>
    <dbReference type="OMA" id="DRRPNAN"/>
    <dbReference type="OrthoDB" id="1936100at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.70:FF:000004">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000004">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050292">
        <property type="entry name" value="Glutamine_Synthetase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852:SF57">
        <property type="entry name" value="GLUTAMINE SYNTHETASE 2 CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00180">
        <property type="entry name" value="GLNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="12">
        <location>
            <begin position="24"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="13">
        <location>
            <begin position="110"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="371" mass="41430" checksum="5EB196254F3DC670" modified="2011-11-16" version="1">MSISITENTAILSKYLDLPQKGKVLAEYVWIDAEGNTRSKCRTLAKAPTSVDDLPEWNYDGSSTGQAPGYDSDVYLRPVAFYPDPFRKGDNVIVLNECWNNDGTPNKFNHRHECAKLMKAHASEEVWFGLEQEYTLFDQYDYPYGWPKGGFPAPQGPFYCGVGTGKVVARDVIEAHYRACLYAGINISGINAEVMPSQWEFQVGPCEGIEMGDQLWVARYLLQRVAEEFGVKISLHPKPLKGDWNGAGCHTNVSTKNMRLPGGMKDIETALSKLAKRHKEHMMLYGADNEQRLTGRHETASIESFSSGVANRGASVRIPRQVAKEGYGYFEDRRPASNIDPYLVTGIMVETICGSIPDADMAKEYLRETSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHS2</accession>
    <name>G3AHS2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59665</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373820.1">
        <property type="nucleotide sequence ID" value="XM_007373758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHS2"/>
    <dbReference type="GeneID" id="18873014"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59665"/>
    <dbReference type="eggNOG" id="ENOG502RM5B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_978477_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHS2"/>
    <dbReference type="OrthoDB" id="4089586at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="246"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="275"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="285" mass="32139" checksum="990817B9FA1A1FA0" modified="2011-11-16" version="1" fragment="single">MEEQGTIEGHYQIRNELDQLQYHDQQRKSLIRLAVLRVHALLRHKHDIPVTKRKSGTPRKNDLEQLRAQLNEYELSINKLKQEHSTQLKLQQAKFDVLKEENSKLKSKLKSQPAQPVTRKFSGGRKPPSLFNITSNYTASPTFVNPAHITTPTRKDGRQNRSFTATHTGLSPTPLAQRSLSLNITKTNDSPSKFVDKFEKSVSPVSSPDFPHRTVNEVPKSATTSLPSSADRINKTFTDDEAYVSANSSVSSMSRESTAALEDNAPKKKKKKLQLWKSKTAKILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHU9</accession>
    <name>G3AHU9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_70401</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373847.1">
        <property type="nucleotide sequence ID" value="XM_007373785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHU9"/>
    <dbReference type="FunCoup" id="G3AHU9">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHU9"/>
    <dbReference type="GeneID" id="18875325"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70401"/>
    <dbReference type="eggNOG" id="KOG3138">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_5_3_1"/>
    <dbReference type="InParanoid" id="G3AHU9"/>
    <dbReference type="OMA" id="EYAGAIC"/>
    <dbReference type="OrthoDB" id="47374at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051556">
        <property type="entry name" value="N-term/lysine_N-AcTrnsfr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919:SF8">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="174" mass="19884" checksum="F6AB2EEB1C4DDA4C" modified="2011-11-16" version="1">MGRDIVALDDLTVNNVGTFQKINEVVLPSRYDAAWYQEAVTPGQITKLAFYAEIPVGAIKARAFNNTHKISSLESLLRFQLPSKNIPNAIYIESFAVLEKYRGLGIGSKLLNYVIEETKSRFIHEIYIHVHVDNKEAIEWYLKKGFVKKDEVIKDYYKSLEIPNPDAYIMTLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIF9</accession>
    <name>G3AIF9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Histone deacetylase HDA1</fullName>
            <ecNumber evidence="3">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_70543</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374013.1">
        <property type="nucleotide sequence ID" value="XM_007373951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIF9"/>
    <dbReference type="FunCoup" id="G3AIF9">
        <property type="interactions" value="166"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIF9"/>
    <dbReference type="ESTHER" id="spapn-g3aif9">
        <property type="family name" value="Arb2_domain"/>
    </dbReference>
    <dbReference type="GeneID" id="18875332"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70543"/>
    <dbReference type="eggNOG" id="KOG1343">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007727_4_2_1"/>
    <dbReference type="InParanoid" id="G3AIF9"/>
    <dbReference type="OMA" id="VIIDWDI"/>
    <dbReference type="OrthoDB" id="424012at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070823">
        <property type="term" value="C:HDA1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000005">
        <property type="entry name" value="histone deacetylase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019154">
        <property type="entry name" value="Arb2-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017321">
        <property type="entry name" value="Hist_deAcase_II_yeast"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF5">
        <property type="entry name" value="HISTONE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09757">
        <property type="entry name" value="Arb2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037919">
        <property type="entry name" value="HDAC_II_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="13">
        <location>
            <begin position="226"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="Arb2-like" evidence="14">
        <location>
            <begin position="598"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="44"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="90"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="142"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF09757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW34429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="891" mass="99332" checksum="66719D368B6A088D" modified="2011-11-16" version="1">MSEPDSLKDDVVPQANEKKVDGDLNVGSGETTSAAEVSTESQQEQDKVAGKASDKQLIEPEEKSKEKEKEKQTTESLHDGAIPENEDVEEKQVDDIVEVVKQEDEETNLEKQDKIKQEEPEPMEIDEVEAKDENLESVSSEIAKDETTSGEPKKHEVSNGEEVEPPHKKIKLEEQSNGINENKIVVVPPTKPHLFYTPLKTGLVYDVRMRYHAKIFTSYFEYIDPHPEDPRRIYRIYKKLAEAGLIQDSSLSGVNDIGPLMLKIPIREATAEEILEVHSESHLKFIESTETMTRDQLMEETEKGDSIYVNNDSYFSARLSCGGTIEACKAVIEGRVKNSLAVVRPPGHHAEPDSPGGFCLFSNVAVAAKNLLKTYPESVRKIVIIDWDIHHGNGTQKSFYNDPRVLYISMHRYENGRFYPGTKYGNSDQVGEEEGEGFNINIPWRSSGMHDGDYVYAFNKVILPTIIEFDPDLIIVSSGFDAADGDIIGGCHVTPAGYGYMTHMLKGIAKGKLAVILEGGYNLDSISKSALAVAKVLVGEPPENTISMQPHLDAVEVVDEVIKIQAKYWKCLRHGIPSTSFDDVFDLPDDTNYKLVNISDTIRSHQNNELFNKYSFINIPIIGTSGSPSDDKGAFTVDLPAQLEDIIIASPDIHNCTTIILSIHDPPDIWANIHPVSGTIEAGSSVILEHPLTKIIDRVAKEESASSERIGYIDINIPSFPLPIPSFTKSPSSSYNPTIFAQELLLYIWDNYLAYFSVKKLVFVGFGDAYQAIVHLFGKRPSSDIKELVKGTIAFVNRTTLKPLVPVMDESMVDWYYQNSVVFTSCLNPCWSGVNGNGKDMDETKRPRRKFGRVLKASGDGLYDVIQERFDEGVDFILDSIEDYSSEESNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIH3</accession>
    <name>G3AIH3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Secreted acid phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_134430</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histidine acid phosphatase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374027.1">
        <property type="nucleotide sequence ID" value="XM_007373965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIH3"/>
    <dbReference type="FunCoup" id="G3AIH3">
        <property type="interactions" value="476"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIH3"/>
    <dbReference type="GeneID" id="18869814"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134430"/>
    <dbReference type="eggNOG" id="KOG1382">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020880_3_1_1"/>
    <dbReference type="InParanoid" id="G3AIH3"/>
    <dbReference type="OMA" id="TGEMDAK"/>
    <dbReference type="OrthoDB" id="6509975at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016274">
        <property type="entry name" value="Histidine_acid_Pase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963:SF18">
        <property type="entry name" value="ACID PHOSPHATASE PHO11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963">
        <property type="entry name" value="MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000894">
        <property type="entry name" value="Acid_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00616">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00778">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="62"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="214"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="263"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="406"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000894-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000894-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="464" mass="51983" checksum="3A3DFFC64C98C7C3" modified="2011-11-16" version="1">MVAISKLINNGLLLAGQAVYQDLATPEQSSVQQYNIIRFLGGSAPYLQRDGFGISVDFPEQCTIEQVQLLSRHGERYPAKSDGANFEPIYQKFVNYNGTFSGQLEFLNDYEYFVPNKNNYEKETSPSNSQGTFSGTSNAFRHGAAFRAKYNSLYKENSTLQVFSSNSGRCYQTSNYFARGFLGDAYEEDETVKYHVISEDASSGVNSLTPRNSCPNYNSTANTGLVAKYNTSSYLQPIADRLVKPNPGLNLTATDVSYLFSWCAYEINVRGASPFCDLFTNEEFIKNSYHTDLSDYYSIGPGHNDSRVIGSTLVNATLALLKDNDNENKIWLSFTHDTDLEFYHSALGLVEPKDDLPVDHIPFPNPYVHSSIVPQGARIETEKLKCGDDYYVRFIINDSVIPIPTCADGPGFSCKLEDYEEYIQSRIGNLDFVEQCNMNSSNPASVSFYWDYNTVTYDAPLGDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AII0</accession>
    <name>G3AII0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DUF726-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_134100</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMCO4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374034.1">
        <property type="nucleotide sequence ID" value="XM_007373972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AII0"/>
    <dbReference type="FunCoup" id="G3AII0">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="GeneID" id="18869782"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134100"/>
    <dbReference type="eggNOG" id="KOG2385">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001695_2_2_1"/>
    <dbReference type="InParanoid" id="G3AII0"/>
    <dbReference type="OMA" id="KFAYIGI"/>
    <dbReference type="OrthoDB" id="277931at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035650">
        <property type="term" value="F:AP-1 adaptor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035652">
        <property type="term" value="P:clathrin-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007941">
        <property type="entry name" value="DUF726"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920:SF3">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4 TMCO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05277">
        <property type="entry name" value="DUF726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="411"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="454"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="565"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="838"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="880" mass="97999" checksum="DB7AC9B51DDF5AF1" modified="2011-11-16" version="1">MSKFHNKTIASELPSLPHTPPILASASHLSNGVTQETETNTDDTTYVEFQLSPPISTNSPRISESVRERVFSSSNINLNEIDEITQPQEPEIKIVEEEEDFFNSDSTNWKEMKAISSMDYYNDKGGLEFTSKSGKEFLHGDKDIFGYTKIDTEEQVNKYASLDKKTDFLFRATHSHRTALAKYEEGEDEKTYDNDSEYEDEDNLDSQEALDDTKNMLTDSQKFAYIGITKLLTVDMATDLAKLHITGSGSIAKILSAGQKNFSNWTLYVMSKLYSHLNISDDEQKMIENLSKHGLEVNDLSNSLLEVGMNASVQANKQCLEEDFDLRWVIICDLFLLLLSDGYYDARSRSLLIKFGKTIDIPYLEIYQFERRLIECLEIETKDRTIENKEELLNDQSLIEKQIKKNRNKRLAYIGLATLGGSLAIGLSAGLLAPVIGAGLAAGLTTVGITGTSGFLAGIGGSAIITTGGIAIGAKVGSKAGARRIGDVHTFEFKPLHNNKRTNLIITVSGWMNGKMDDVRLPFSTVDPVMGDMFSLLWEPEMLQSMGQTIGILASEALSTSIQQILGATVLTALMSAIQIPMILSKLSYLLDNPWNVSLDRAWKAGKILAETLIAGNVGVRPITLVGFSLGARVIYSCLIELARRGGYGLVENVIILGSPISIKTDQLIMAKSIVSGRFVNGYSKKDWILGYLFRATGGGVGKVAGLSALTHVYGVENIDCTSLVEGHMSYRKAIPKILKLVNWEVLSEEFAEIEEPDPEQGERQRQLIHEFDEARAKMEKEKQEAVEPKGWKKWFKPKNKDWWEIYGQNSANNSKEDVNNSNPNDEYQVEEDVVFNVDALAQEVNEIENLAQKDKQSIEELRKNVESVPSKIVLNEDME</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AII1</accession>
    <name>G3AII1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="5">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phenylalanyl-tRNA synthetase beta subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_133462</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="5"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374035.1">
        <property type="nucleotide sequence ID" value="XM_007373973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AII1"/>
    <dbReference type="FunCoup" id="G3AII1">
        <property type="interactions" value="1116"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AII1"/>
    <dbReference type="GeneID" id="18869752"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133462"/>
    <dbReference type="eggNOG" id="KOG2472">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020279_2_0_1"/>
    <dbReference type="InParanoid" id="G3AII1"/>
    <dbReference type="OMA" id="FPGRCAN"/>
    <dbReference type="OrthoDB" id="1698572at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.40.10:FF:000002">
        <property type="entry name" value="phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.10:FF:000006">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.10:FF:000004">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000052">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="entry name" value="Phenylalanyl-trna Synthetase, Chain B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
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        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
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        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
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        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
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        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
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        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
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        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
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        <property type="entry name" value="PhetRS_B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947:SF0">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN AND LEUCINE-RICH REPEAT-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18262">
        <property type="entry name" value="PhetRS_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 18" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B5" evidence="17">
        <location>
            <begin position="290"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW34451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="591" mass="66788" checksum="F10C88E3B519C93B" modified="2011-11-16" version="1">MPTIPVDKEDLYKLLGRSYTTEEFDELCFDFGLELDEDTTDDCQGDERPQLKIEVPANRYDMLCIEGIAQALNEFLGNVEPPKYTLNPAKPVTSLTIKESTLPIRQYAASAILRNVTFDERTYDSFIAFQDKLHTNLCRNRTLVAIGTHDLDTLTPPFTYEALAPEDIKFKPLNQDKEMNGHELMEFYEKDKNLGKYLHIIRDSPVYPVMLDANRTVASMPPIINSDHSKITLNTKNVWIDVTGTDKTKTEVVIEQIVAMFSRYCKTPFEIEPLDIISEHNNESRITPNIAPRVAQAEISYINSCLGLDYSGEEISKLLKKMALEASQSKEDKDLIDVKVPITRSDVLHQCDIMEDAAIAYGYNNLTKTKPKGESLVAAPLPINKVADILRLASAQAGYLEVMPLTLCSHDENFKFLRQSDDGTKAVKLANPKTIEYQVVRTTLLPGILKTIKENRKHSLPIKVFESGDIVLKNPKLERKAFNQRNWCAIYAGKTSGFEYVQGLLGKIMQTMRTEWLENPKENKGRGYWIEEDKDNATFFPGRGAKIFFRAHEGGEDQHIGALGVLHPEVMTSFEIPYAASSVEINAEVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIM1</accession>
    <name>G3AIM1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial splicing suppressor 51-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59099</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007373258.1"/>
    </dbReference>
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        <property type="interactions" value="155"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIM1"/>
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    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59099"/>
    <dbReference type="eggNOG" id="ENOG502QQBW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1038874_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIM1"/>
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    <dbReference type="OrthoDB" id="5282002at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046824">
        <property type="entry name" value="Mss51-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28069">
        <property type="entry name" value="GH20023P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28069:SF1">
        <property type="entry name" value="PROTEIN MSS51, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20179">
        <property type="entry name" value="MSS51_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mitochondrial splicing suppressor 51-like C-terminal" evidence="1">
        <location>
            <begin position="17"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="268" mass="31212" checksum="A5B004091CF44BFA" modified="2011-11-16" version="1">MNDEFNLAASTKILTYPMTIASLLHKNSPYQQEPKGPITLEGLKSLSALRYTLYPPHAKSAETDVVYRERPMRIFILGSRMESMLPGYVWKQFGYLFPGTRFQIHLVGPEAYFDRETKAFAPTNVPQGRPLVERVDDQISIFHHSQYFHELYDMGDLFPFDPYLDVFFLFHPGFQTADHIHWDKSLKGLLESKCPVFVSGYHNADMDKEIEWLNKHPLHEEMDIMMNKTDNLFACTKLELDDAHPTETFNLNNKLFGFRGKRYHAIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIZ6</accession>
    <name>G3AIZ6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_59943</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sphingosine N-acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374092.1">
        <property type="nucleotide sequence ID" value="XM_007374030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIZ6"/>
    <dbReference type="FunCoup" id="G3AIZ6">
        <property type="interactions" value="587"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIZ6"/>
    <dbReference type="GeneID" id="18873144"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59943"/>
    <dbReference type="eggNOG" id="KOG1607">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028277_4_0_1"/>
    <dbReference type="InParanoid" id="G3AIZ6"/>
    <dbReference type="OMA" id="YLIGAPY"/>
    <dbReference type="OrthoDB" id="3053196at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050291">
        <property type="term" value="F:sphingosine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016439">
        <property type="entry name" value="Lag1/Lac1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560:SF11">
        <property type="entry name" value="CERAMIDE SYNTHASE LAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560">
        <property type="entry name" value="LONGEVITY ASSURANCE FACTOR 1 LAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="249"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="297"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="353"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="12">
        <location>
            <begin position="165"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="387"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="390"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="408" mass="48172" checksum="1E9F9A866211C561" modified="2011-11-16" version="1">MSALRVPEDETTHRRRGSSVGHINVGDTASIGFSTLPSTKQQRKRSRSRLVELESHGKSDTAIVKKIIIAFQELTYRHTWIYPLIYLSGILSTFYLSTPESKTHQVLAEFIVPSYHIEGTDQYGKGGNDFKFVAFYALFFTFYREFLMCCFLRPIAKWLGISRASKQKRFMEQTYSMVYYGVAGPVGLWIMSRLPLWYFNTTQFYVAYPHKTHDVFFKCYYLGQAAFWVQQSVVLVLQLEKPRKDFKELVFHHIVTIALIWCSYRFHFTWMGLAIYITMDISDFVLATSKTLNYLDSPITGPFFVVFIGVWVYLRHYLNLKILWSVLTEFRTVGEWELNWDTQQYKCYISQPITFFLIFALQLVNIYWLFLIFRILYRYVFVGETKDERSDDEEEDEVSSEEETKKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ36</accession>
    <name>G3AJ36_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_59975</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374132.1">
        <property type="nucleotide sequence ID" value="XM_007374070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ36"/>
    <dbReference type="STRING" id="619300.G3AJ36"/>
    <dbReference type="GeneID" id="18873161"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59975"/>
    <dbReference type="eggNOG" id="ENOG502QVE1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006698_1_0_1"/>
    <dbReference type="InParanoid" id="G3AJ36"/>
    <dbReference type="OMA" id="LRVEFNY"/>
    <dbReference type="OrthoDB" id="4089008at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase III" evidence="2">
        <location>
            <begin position="114"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="487"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="314"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="494"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="511" mass="57217" checksum="AFC90B8BF4137596" modified="2011-11-16" version="1">MDRDHKRIKTGDDHENQSFLSEIAHVDPNEVYDVNYSRTKSITSDDRAKETIDSMVTPRIITGNTYSNNHSMIGGAMLPPIANLTMNEEVVNIPNCQAAKQNLLNKIERSKTCNESAVLKKSDNIQFLGKSVMNCCIAYLFEKSFSKINEGSIEYLRNKLTTESSLAKFFRELDPKEALLNNTISDEVALITLFTLIGGCVNDFGFEQVLLPLCEIMYVSVLQEYPLIVKHSVPFKAEQYLQNTAPGASSSLNSLAEVAAADVHNTHSHLPVNPAITSGRSSAIQNLSPTPSAGIPPIRSISPELEMRSRLQNPQPQGQSQQQQQQPPPPPQHIPHISPQPQAQLRDRKRVFIHFLNQVLEFTYPIRNSAPPRYNELIENARIAFKLPSSSDSILGLRNKVDRMILQTDFDLERIFRGDDPIELEIFTQRTKAIPIYELTSTVISNGSMDAINRTHTESPKIILPPPINTTHAYGNTSEFNFLSNSDDIHRITTPPPPPPQPILPKFQPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ72</accession>
    <name>G3AJ72_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="8">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_59187</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[pyruvate dehydrogenase E1 alpha subunit] + ATP = O-phospho-L-seryl-[pyruvate dehydrogenase E1 alpha subunit] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23052"/>
            <dbReference type="Rhea" id="RHEA-COMP:13689"/>
            <dbReference type="Rhea" id="RHEA-COMP:13690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="8"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373413.1">
        <property type="nucleotide sequence ID" value="XM_007373351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ72"/>
    <dbReference type="STRING" id="619300.G3AJ72"/>
    <dbReference type="GeneID" id="18872814"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59187"/>
    <dbReference type="eggNOG" id="KOG0787">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023861_5_1_1"/>
    <dbReference type="InParanoid" id="G3AJ72"/>
    <dbReference type="OMA" id="NEMPSIC"/>
    <dbReference type="OrthoDB" id="241648at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16929">
        <property type="entry name" value="HATPase_PDK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000065">
        <property type="entry name" value="[Pyruvate dehydrogenase (Acetyl-transferring)] kinase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF3">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE (ACETYL-TRANSFERRING)] KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="10">
        <location>
            <begin position="353"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="153"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="512" mass="57694" checksum="FB52436314113B2E" modified="2011-11-16" version="1">MGTGWELTSALKSKIFQYAAHNQTPVSLRQMVQFGPSPSPGSIFLASKFIVEELPIRLAKKVKDLEYAPQGLNESPSTIKVKNWYAQSFQELTELPKPKIDEKLASMLFKNGVHRYEADESGELHHSTNYEDNPAINNVFSDDGMVLRQTHQHGYGHGHSHSHSHSHIQSSPAIPAKENSPTFGMTYFNPCPMNIIWPREVYAYNKLVCDALTKIKLRHDATVATMAQGVQEWKERNKAGSIINSQIQTFLDRFYMSRIGIRMLIGQHIALNMSQSSPTKQRINSFLNGTQGNNQTSNSRLNYVGVICTDCNVAEIAEDAIQTASYICEEYYGLFEAPEIQLVAPHHAINFMYVPGHLIHMLFETLKNSLRATIEFHTPLLKQKMIEENPGMKYDDIDLNDLKFPPIKVIISEGSQDITIKISDEGGGIARREVPLIWTYLYTTVHETPKLEPEFNQTSFKAPMAGFGYGLPISRLYAQYFGGDLKLISMEGYGTDVYLHLNRLSSSSEPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJB9</accession>
    <name>G3AJB9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nucleosome assembly protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_60008</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the nucleosome assembly protein (NAP) family.</text>
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        <property type="protein sequence ID" value="EGW34578.1"/>
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    </dbReference>
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        <property type="project" value="EnsemblFungi"/>
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    </dbReference>
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        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of microtubule polymerization"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:protein localization to cell division site after cytokinesis"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:ribosomal small subunit biogenesis"/>
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    </dbReference>
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        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
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    </dbReference>
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        <property type="entry name" value="NAP-like"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
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        </location>
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        </location>
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        <location>
            <begin position="371"/>
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
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        <location>
            <begin position="16"/>
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        </location>
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        </location>
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        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
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    <evidence type="ECO:0000256" key="4">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="411" mass="47653" checksum="A8D248ACC228BBEA" modified="2011-11-16" version="1">MSEPIKKKNGDISKAPTPQNTPSSVGTSYLRSQPPTVSTIEEINEDNVGKQIANNPALLSMIQGKLGDLVGKTSGYVEHLPKKVRNRVSGLKGIQSAQMKLEADFQKELLSLEKKYFKLYEPLYEKRKKIINGDLEPTAEEVEEGEALEEKDEEEEEEEEEQEEEEEEEDDEEQKGIPGFWLTALENLTTVSETITDRDSDVLQYLSDIRMEYLETPGFELIFEFQPNEFFSNQILTKTYHYQAELGYSGDFVYDHADGCEINWKSKENNVTITIERRKQRNKTTKQTRTIEKLTPTESFFNFFDPPKPPKVDKEDEEKDEDDEEHEEDFEDVDEDLEARLELDYQLGEEIKDRLIPRAIDWFTGDAVDFAYPEDLEDDEGEFSDEDEDDDEDDEDDNDQPKQQPPECKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
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        <name evidence="3" type="ORF">SPAPADRAFT_60045</name>
    </gene>
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        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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    <dbReference type="HOGENOM" id="CLU_1349641_0_0_1"/>
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    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442361" evidence="2">
        <location>
            <begin position="17"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="184"/>
        </location>
    </feature>
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        <location>
            <begin position="129"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="203" mass="20373" checksum="D6C9DF1863542646" modified="2011-11-16" version="1">MKYLTALALLTALVAAQDAKPSSTSTTQEPCVTEGPCADAIALQMASCKQENLSEDSLKIGECTCKLDANYWETLSNCVQGCPEFSPHAPKNDAASLQKYQCDALLPYASGASSEIDDASTTEGVKDVTSSAKTTGSGSATKATTSAETTTTKASSGTTATTKTTGSGSATGAAETDSTTSTKNGAAGAGLGSLIYLVAAAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJF5</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
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    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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            <taxon>Pichiomycetes</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR018854">
        <property type="entry name" value="Psome_chaperone_3/4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10448">
        <property type="entry name" value="POC3_POC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="107" mass="12089" checksum="17383929E7201D8F" modified="2011-11-16" version="1">MNHIVKEVNLNGIDYTFIISYNHEINPKAPITIYINQTNNQAMGSYVYTINSYSTNLVPGSNTEQIISLNNLLTKKFQVPIYLNINGNIDCSNVELFTTIVDTIESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJL2</accession>
    <name>G3AJL2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_59287</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373498.1">
        <property type="nucleotide sequence ID" value="XM_007373436.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJL2"/>
    <dbReference type="FunCoup" id="G3AJL2">
        <property type="interactions" value="587"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJL2"/>
    <dbReference type="GeneID" id="18872854"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59287"/>
    <dbReference type="eggNOG" id="KOG0700">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021928_3_2_1"/>
    <dbReference type="InParanoid" id="G3AJL2"/>
    <dbReference type="OMA" id="DSLRTYW"/>
    <dbReference type="OrthoDB" id="420076at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004741">
        <property type="term" value="F:[pyruvate dehydrogenase (acetyl-transferring)]-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF792">
        <property type="entry name" value="GM14286P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="6">
        <location>
            <begin position="156"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="447" mass="50184" checksum="5E4E181B91B3CE70" modified="2011-11-16" version="1" fragment="single">MVYVLAVKPRFLYVTAKQLQRMAYNKHHIANFARNFSTHQIPHNVKRITTSSSSSRDYKKKVALVSGVIIIGSFISFSHSDYYNLDTIPTVAKPIEKNSTPDGKRMFSTSTSSLLQNPTSKQGIYLLTEDQVSEKLRQFEESYLVNRGRGVTRYDICQLASNSPIEDDRAEEIVQVQLLQDNNIRASTDWMFFGVFDGHGGWTTSSKLRDKLIGYVIHELGTIFKSVKSGDTEEEVRLIPNSATIDQAIKNGFLKLDHELVNENIAKLLNENNKAKAAELLMPALSGSCALVSFYDTHSKILKVAVTGDSRAILGSFRDNQWTVRQLSIDQTGSNPTEVARIISEHPDEPKVIKNGRVLGSLEPTRAFGDCRYKLPGSIQEKIYKQFFGKKLPNFLTSPPYVTAEPVITSTKVDPDNNDFLVMASDGLYEMLTNEEIVGLVVKWMEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJY8</accession>
    <name>G3AJY8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1892 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59464</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373623.1">
        <property type="nucleotide sequence ID" value="XM_007373561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJY8"/>
    <dbReference type="GeneID" id="18872926"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59464"/>
    <dbReference type="eggNOG" id="ENOG502S3ZA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126651_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJY8"/>
    <dbReference type="OMA" id="GHIKYEV"/>
    <dbReference type="OrthoDB" id="4035606at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.250">
        <property type="entry name" value="YML108W-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015080">
        <property type="entry name" value="DUF1892"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035946">
        <property type="entry name" value="YML108W-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08987">
        <property type="entry name" value="DUF1892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89975">
        <property type="entry name" value="Hypothetical protein Yml108w"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="116" mass="13270" checksum="D3BDCB33B401DA7F" modified="2011-11-16" version="1">MSDTEFEQPENLDNPLRIIIVLNSPSHPDGEMQDRLLDTIQSMEEVNDFFDEFDSKVCIPNEGHIQYEVGSDGMVVVLVDSEELMGDALKFIDGYSTVENEEQDDEERDGKKKKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK69</accession>
    <name>G3AK69_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60224</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374395.1">
        <property type="nucleotide sequence ID" value="XM_007374333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK69"/>
    <dbReference type="GeneID" id="18873282"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60224"/>
    <dbReference type="eggNOG" id="KOG0401">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_822745_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK69"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="117"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="143"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="262"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="338" mass="35918" checksum="E6F06002F9335F7D" modified="2011-11-16" version="1" fragment="single">MTDNNPKPDTSSGSVSASTSADNTNTTTTPNNGGGNSGSTTSNVDSGVAHQQQQPSSIQSGSSGSTPQNQYANLNYQQSYQQHANYNPNYTPNYGGYSGNYSGKNYNKNYSARQSYNPNNNNSINSNTNTGNQRYNNANSQISGSAGSTGSNISNSNKKSYNNQKLNQQQQQQQQQQQQQQAMQQGYPGYAYGSYPYYISGYPQMYVPYGAMPAQTYVPPIPQGLQPQPQHPVSTTPPITKVKLTTKDGKLVDLEEKKKKTTASSTPVSSPHPTAATPNTGVKSSASASPRPQEPATVSTPTTTTTTTTAAASAAASDNTVSAAAAAAEEFKRKIKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK73</accession>
    <name>G3AK73_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60226</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374399.1">
        <property type="nucleotide sequence ID" value="XM_007374337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK73"/>
    <dbReference type="STRING" id="619300.G3AK73"/>
    <dbReference type="GeneID" id="18873284"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60226"/>
    <dbReference type="eggNOG" id="ENOG502QUF6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_821752_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK73"/>
    <dbReference type="OrthoDB" id="774557at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029005">
        <property type="entry name" value="LIM-bd/SEUSS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01803">
        <property type="entry name" value="LIM_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="292"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="338" mass="37094" checksum="A25BA462FDFC2AEC" modified="2011-11-16" version="1">MPTYFSPSSVIKFTTAPTQNKNALLEKSGLKLPFLDLGNGMNSPQNTSHQFELSSIVASRFMLACIAANNVVRFTISLPGLKSQVMNNGSIFLMARMMIQYIYSDGSISNINGNIRVLMGRDLAIEWVDCQCLNYQSSLALSGLERKWANFLDSKAKDPKKGDKDFLNDIYENTEAVKLFASSGIHQDAMRIMQVGDVMSNLKSLMEFDQVNNISSPMKSLELYVSRNSGQIALQSQLAQAQFQAQVQAQVHAKAQAQAQAQAQAHAAQAAQAAQAQFQAQAYQVQMAAQQASSAPVPVPQQSRNSTISSPSPRTIHAEDPKKKRKASTTIADNKRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKC7</accession>
    <name>G3AKC7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_66528</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375101.1">
        <property type="nucleotide sequence ID" value="XM_007375039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKC7"/>
    <dbReference type="GeneID" id="18875104"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66528"/>
    <dbReference type="HOGENOM" id="CLU_1579485_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKC7"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="169" mass="18998" checksum="C8DEA78DF2583CF7" modified="2011-11-16" version="1">MASNNTKDSTAAKNTTNALTFTFRKRSHALALYNHLQVSMDSDEINAPSFPDYIMKELRYISQNRHNNQVNTDTAPIENGDHHAEERNNPSSLHQAPVPLELGTIANNVGRALLEKTTSREEIEHHEQKLVRKSGNRDRKRARLGKAFIGMVGVVDIANPINTFAKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKE0</accession>
    <name>G3AKE0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_60240</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374412.1">
        <property type="nucleotide sequence ID" value="XM_007374350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKE0"/>
    <dbReference type="FunCoup" id="G3AKE0">
        <property type="interactions" value="426"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKE0"/>
    <dbReference type="GeneID" id="18873292"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60240"/>
    <dbReference type="eggNOG" id="KOG2495">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019845_6_2_1"/>
    <dbReference type="InParanoid" id="G3AKE0"/>
    <dbReference type="OMA" id="WKIGAPR"/>
    <dbReference type="OrthoDB" id="202203at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735:SF3">
        <property type="entry name" value="FERROPTOSIS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="5">
        <location>
            <begin position="6"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW32897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="372" mass="40518" checksum="6DEB0ABAE2772289" modified="2011-11-16" version="1">MTTNKKKVIIIGASYGGILALKTLLSTKKCHTKDISLDITIIAPNDHTYFNIASPRLLTELNLASKVVFKIKDVIEKLSRGTEHTVGFVQGSVQKVDLDDKSLTILNSDRTFNYDNLIIASGTRTDFPGFKLDNQKDQTYSIEAIKILSKQIEDAKSIAIIGGGSTGVEVAGELGYKYGKQKEEIVLYTGASRPLPTLPENQSSKATTKLERLGVTIVNDKVNVRENSVELANGSIVKYSLVITAYKHIPNTEFLPANVLNKTGYVITDSHLRLKQHPEVICVGDALEIGQRSVVDLVYGQKSVIESTIQVEVFGNASKPKSYEAPKSTTMVVPIGKEGGVGLAFGWNVPNFLVSILKAKDFMISKAGDNLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKM5</accession>
    <name>G3AKM5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NADPH-dependent diflavin oxidoreductase 1</fullName>
            <ecNumber evidence="9">1.18.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">NADPH-dependent FMN and FAD-containing oxidoreductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TAH18</name>
        <name evidence="12" type="ORF">SPAPADRAFT_135972</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of DRE2, another key component of the CIA machinery. In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins. Positively controls H(2)O(2)-induced cell death.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2 oxidized [2Fe-2S]-[protein] + NADPH = 2 reduced [2Fe-2S]-[protein] + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67716"/>
            <dbReference type="Rhea" id="RHEA-COMP:17327"/>
            <dbReference type="Rhea" id="RHEA-COMP:17328"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with DRE2; as part of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Mitochondrion</location>
        </subcellularLocation>
        <text evidence="9">Relocalizes to mitochondria after H(2)O(2) exposure.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the NADPH-dependent diflavin oxidoreductase NDOR1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the N-terminal section; belongs to the flavodoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.18.1.-" evidence="9"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375145.1">
        <property type="nucleotide sequence ID" value="XM_007375083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKM5"/>
    <dbReference type="FunCoup" id="G3AKM5">
        <property type="interactions" value="744"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKM5"/>
    <dbReference type="GeneID" id="18869934"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135972"/>
    <dbReference type="eggNOG" id="KOG1159">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_17_6_1"/>
    <dbReference type="InParanoid" id="G3AKM5"/>
    <dbReference type="OMA" id="DIMSIPR"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160246">
        <property type="term" value="F:NADPH-iron-sulfur [2Fe-2S] protein oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006809">
        <property type="term" value="P:nitric oxide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:positive regulation of nitric oxide biosynthetic process"/>
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        <property type="project" value="EnsemblFungi"/>
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    </dbReference>
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        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
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    </dbReference>
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        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
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        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
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        <property type="entry name" value="NADPH-DEPENDENT DIFLAVIN OXIDOREDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FLAVODOXIN"/>
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        <property type="entry name" value="FPNCR"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="6 9" id="KW-0274">FAD</keyword>
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    <keyword evidence="5 9" id="KW-0288">FMN</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 9" id="KW-0521">NADP</keyword>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="10">
        <location>
            <begin position="5"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="11">
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            <begin position="193"/>
            <end position="430"/>
        </location>
    </feature>
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        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>FMN</name>
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        </ligand>
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        <location>
            <begin position="59"/>
            <end position="62"/>
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        <ligand>
            <name>FMN</name>
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            <position position="132"/>
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        <location>
            <position position="340"/>
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            <begin position="371"/>
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        </ligand>
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            <position position="445"/>
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            <begin position="499"/>
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            <position position="581"/>
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            <name>FAD</name>
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        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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            <dbReference type="PROSITE" id="PS50902"/>
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            <dbReference type="PROSITE" id="PS51384"/>
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        <source>
            <dbReference type="EMBL" id="EGW33630.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="581" mass="67170" checksum="132DDB144184F3E7" modified="2011-11-16" version="1">MSDQITILYGSETGNAEEYARYLHYRLRRMNLKPTLSQLDEYPLKQLITNTTHLIVICSTTGQGELPRNSKKFMKFILKKKLPYDLFQHLRLTTLGLGDSSYTKFNYAIRKIHARLVQLGCQELSPRCEADEMSAEGTDGYYQEWENQLIEALQKDFPKATGINEDLLLMPQYKVSTKAQSLEQDFAQLKVSPDLQAGTVVSNDRITTPDHFQDVRDFRIKSTDLDYLPGDTVSLYPANMDEDVEALLQSQPTWLKIADKPLTISSPPKIEGGFIPNLTLRKLIKYHLDIISIPRRSFFSLLWHFVDPSTEDGQREQEKLQEFSNFEDPELLYDYANRPRRSILETVVEFENNLTIPVNYVLDLFPLIKPRMFSIASKPSSTELELVVAIVEYKTIIRRIRRGLCTRWIKSLKPGDLVRFSIQKSSFNYRNENSAPPIIMVAPGTGVAPMKSLIGQVLSEQTNQELYLFFGCRFKDKDHLIDTFWPESYPNLHIFNCFSRDKDSKYKYVQDALFDQYKLMGDLILNENAKIFVCGSSGKMPREVKLTFAEIVKKYSNISDEEAQRYIISLEDNGRYKEDAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKZ8</accession>
    <name>G3AKZ8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_60358</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EBP2 family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_007374494.1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
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    </dbReference>
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        <property type="term" value="P:nuclear division"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="Ebp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028">
        <property type="entry name" value="RRNA PROCESSING PROTEIN EBNA1-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028:SF0">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EBP2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ebp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="39"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
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            <begin position="82"/>
            <end position="105"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="114"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="301"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="316"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="316" mass="36649" checksum="FB2F6FC626F0F02F" modified="2011-11-16" version="1" fragment="single">MAKGGLLKQQLKNQQLVDAIDKARSKPVIKQKEEIVVKEKVEAPKPILKQKKEKKVKKDEYQSDVLSKKEQRRLKKLQAEQEKEEEEEEEQEDDENEDEDEEELDLERLAASDSESDINGEDDDEEDDEDDEDQEEEDDNEEVEEEEEDVPLSDVEIDEDADVVPHTKLTINNMAALRESLARIELPWDKHAFTEHQSVVSAEKSEAGIKDIYDDTERELAFYKQGLDAVKQARVTLLKLKVPFSRPMDYFAEMVKSDEHMDKLKSKLLTEAANKKASEEAKKQRHLKKFGKQVQNATLQERAKQKRETLEKIKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALK4</accession>
    <name>G3ALK4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_60592</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374762.1">
        <property type="nucleotide sequence ID" value="XM_007374700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALK4"/>
    <dbReference type="STRING" id="619300.G3ALK4"/>
    <dbReference type="GeneID" id="18873480"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60592"/>
    <dbReference type="eggNOG" id="ENOG502QW7T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050100_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALK4"/>
    <dbReference type="OMA" id="QIFAWMS"/>
    <dbReference type="OrthoDB" id="3366093at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005034">
        <property type="term" value="F:osmosensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000282">
        <property type="term" value="P:cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006972">
        <property type="term" value="P:hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007232">
        <property type="term" value="P:osmosensory signaling pathway via Sho1 osmosensor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001402">
        <property type="term" value="P:signal transduction involved in filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039295">
        <property type="entry name" value="MSB2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35778">
        <property type="entry name" value="SIGNALING MUCIN HKR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35778:SF1">
        <property type="entry name" value="SIGNALING MUCIN HKR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="444" mass="45635" checksum="37A49089410B796F" modified="2011-11-16" version="1">MQTGSITSLTSADSSADATATGSESPSDTIGQSVTVSGSLSSETSAPSTGSSGVESAVRPTESGVNTSEGESSFVSATSSFLNGTEPGVTAPTTLIETSQPAEQPQQQPTTTTTVVPHPPTQTPATSNWLPTVLVTAATATDEGTLTNSDIVQATGTAATSGLPRAITPADPIKPAPDYQVITIGFKAPLNYPFVVKNSISSAQIFQFLPEVLKFPFISTHHDSSIYKDVSVNRLIPFTSSQVSYTITVAEVYFPSSAIAALSDLIISPGSLIYRNPDSTQNTLASLIDARVALVGLATSSGSGSGNGAGDDAESNPLLKYHGSMDSEANAAAESSDNVSSGRVAAIAVGAASGCGIYLSLMLFLFKRYKNNSGNIELESTDSESRLGMSEVDSRGTSSGSPTFSGMFGRFSDRENGSHSGSDSSARGKISVPVKASNSLGWYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALQ3</accession>
    <name>G3ALQ3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I and III subunit RPAC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">DNA-directed RNA polymerases I and III 40 kDa polypeptide</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_60633</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374811.1">
        <property type="nucleotide sequence ID" value="XM_007374749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALQ3"/>
    <dbReference type="FunCoup" id="G3ALQ3">
        <property type="interactions" value="1030"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALQ3"/>
    <dbReference type="GeneID" id="18873498"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60633"/>
    <dbReference type="eggNOG" id="KOG1521">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038421_0_1_1"/>
    <dbReference type="InParanoid" id="G3ALQ3"/>
    <dbReference type="OMA" id="KKKCRAF"/>
    <dbReference type="OrthoDB" id="270173at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07032">
        <property type="entry name" value="RNAP_I_II_AC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.120.12:FF:000003">
        <property type="entry name" value="Dna-directed rna polymerases i and iii subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.10:FF:000005">
        <property type="entry name" value="Dna-directed rna polymerases i and iii subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001514">
        <property type="entry name" value="DNA-dir_RNA_pol_30-40kDasu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033901">
        <property type="entry name" value="RNAPI/III_AC40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001988">
        <property type="entry name" value="PRK00783.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF13">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00446">
        <property type="entry name" value="RNA_POL_D_30KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="8">
        <location>
            <begin position="52"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00081520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="335" mass="37714" checksum="50EC4175D6478521" modified="2011-11-16" version="1">MSDNIVGIEYNRVTNTSSTDFPGHQASGDFSWDIEKFKQNFDIDIKYLTERTANFDLVHIDTSIANAFRRIMIAEVPSVAAEAVYMFMNTSVIQDEVLAHRIGLIPLKVDPDLLSWVDKTQDENNRYTEDNTIVLSLDVVCTKNPNAPKNSTNAKELYRNSHVYASDLKFEPHGSQLEKFKNNPVVPADPNILLAKLRPGQEISLRAHCILGIGSDHAKFSPVATASYRLMPVIDIREPITGALAVNFQKCFPPGVIGINDQGEAYVQDARKDTVSREVLRHEEFDGKVKLGRKRDHFIFNVESTGAMSPEEIFFKSVRVLKNKVEYLRNCPIGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALT5</accession>
    <name>G3ALT5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Fungal transcriptional regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GAL4</name>
        <name evidence="12" type="ORF">SPAPADRAFT_137810</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374210.1">
        <property type="nucleotide sequence ID" value="XM_007374148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALT5"/>
    <dbReference type="STRING" id="619300.G3ALT5"/>
    <dbReference type="GeneID" id="18870076"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137810"/>
    <dbReference type="eggNOG" id="ENOG502SST6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_197198_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALT5"/>
    <dbReference type="OMA" id="DFPRCTK"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000435">
        <property type="term" value="P:positive regulation of transcription from RNA polymerase II promoter by galactose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.10:FF:000009">
        <property type="entry name" value="C6 transcription factor (Gal4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051127">
        <property type="entry name" value="Fungal_SecMet_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF3">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="11">
        <location>
            <begin position="29"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="79" mass="9011" checksum="7EF7C54864C1B324" modified="2011-11-16" version="1" fragment="single">MTEKDIESGEVEIIAPKVTKSSNPAIEQACDSCRKRKLKCSKDFPRCTKCTQHNWNCSYSPRTVRSPLTRAHLTEVENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALZ8</accession>
    <name>G3ALZ8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Diphthamide biosynthesis protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_60689</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2 [3Fe-4S](0)-[protein] + 2 Fe(2+)-[Dph3] + NADH = 2 [4Fe-4S](1+)-[protein] + 2 [Dph3] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:71239"/>
            <dbReference type="Rhea" id="RHEA-COMP:17997"/>
            <dbReference type="Rhea" id="RHEA-COMP:17998"/>
            <dbReference type="Rhea" id="RHEA-COMP:18001"/>
            <dbReference type="Rhea" id="RHEA-COMP:18002"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:33723"/>
            <dbReference type="ChEBI" id="CHEBI:47402"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[3Fe-4S](1+)-[protein] + Fe(2+)-[Dph3] = [3Fe-4S](0)-[protein] + Fe(3+)-[Dph3]</text>
            <dbReference type="Rhea" id="RHEA:71235"/>
            <dbReference type="Rhea" id="RHEA-COMP:17996"/>
            <dbReference type="Rhea" id="RHEA-COMP:17997"/>
            <dbReference type="Rhea" id="RHEA-COMP:18002"/>
            <dbReference type="Rhea" id="RHEA-COMP:18003"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:33751"/>
            <dbReference type="ChEBI" id="CHEBI:47402"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DPH3 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374866.1">
        <property type="nucleotide sequence ID" value="XM_007374804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALZ8"/>
    <dbReference type="FunCoup" id="G3ALZ8">
        <property type="interactions" value="349"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALZ8"/>
    <dbReference type="GeneID" id="18873525"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60689"/>
    <dbReference type="eggNOG" id="KOG2923">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155991_4_1_1"/>
    <dbReference type="InParanoid" id="G3ALZ8"/>
    <dbReference type="OMA" id="LFTYPCP"/>
    <dbReference type="OrthoDB" id="66964at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.660.10:FF:000001">
        <property type="entry name" value="Diphthamide biosynthesis 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.660.10">
        <property type="entry name" value="DPH Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044248">
        <property type="entry name" value="DPH3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007872">
        <property type="entry name" value="DPH_MB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036671">
        <property type="entry name" value="DPH_MB_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454:SF31">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454">
        <property type="entry name" value="DPH3 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05207">
        <property type="entry name" value="Zn_ribbon_CSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144217">
        <property type="entry name" value="CSL zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51074">
        <property type="entry name" value="DPH_MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DPH-type MB" evidence="8">
        <location>
            <begin position="5"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="73" mass="8457" checksum="95A05F8C08F3C8AC" modified="2011-11-16" version="1">MSETIYDEIEIEDFTFDPVTQLFQYPCPCGDRFAVALDDLEDGEDIAVCPSCSLMVRVIFEPEDLEEFREQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM30</accession>
    <name>G3AM30_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_149868</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374250.1">
        <property type="nucleotide sequence ID" value="XM_007374188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM30"/>
    <dbReference type="FunCoup" id="G3AM30">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM30"/>
    <dbReference type="GeneID" id="18870846"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_149868"/>
    <dbReference type="eggNOG" id="ENOG502RYS8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_713701_0_0_1"/>
    <dbReference type="InParanoid" id="G3AM30"/>
    <dbReference type="OMA" id="PEHAKQF"/>
    <dbReference type="OrthoDB" id="4073963at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="38065" checksum="ECD668E88BCAEFBD" modified="2011-11-16" version="1">MFKSMVQTDSSSSIRFPSFSRAGLVSDEQKYSGHTVGSRRSNFLKVCSWPFSRSEQESTSTVNNTGYDQSDEEDRAATITPRRKGRFQNPLVGINPSVENINQNNHSEKLLKTLTRKPHESSHRKRVVVLTNVDSSMGINSILQQVCGGPLEKVVQLNNGHIELYFIFPEHAKQFYTYGRATGLLMVNGQRLRVEWWMNENENVNYHPPISKPLLMEIIQHGCRRCLILAKEVRGKQLRSGDKMFYPEPTIHYSQDLNIEDVKADFGRFGKILDIGSVISRKLCFSIFYSDVRSAIFAMNEIETGGSDLNKKYRDWFIWYGKDVTDRACFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM41</accession>
    <name>G3AM41_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial group I intron splicing factor CCM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_65790</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374261.1">
        <property type="nucleotide sequence ID" value="XM_007374199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM41"/>
    <dbReference type="STRING" id="619300.G3AM41"/>
    <dbReference type="GeneID" id="18875063"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65790"/>
    <dbReference type="eggNOG" id="ENOG502QSY4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015498_0_0_1"/>
    <dbReference type="InParanoid" id="G3AM41"/>
    <dbReference type="OMA" id="THLIMEL"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="914"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="914"/>
            <end position="930"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="966" mass="110704" checksum="8BE1AEC7B832AECE" modified="2011-11-16" version="1">MIRLSLLRQGSIPARYLTRVRGISTSSIATATSLPPGSVNKPNRDSNQGFKDNKFNNKKYNKNHRYKHNSNDTGAFNANPHRQRDGKHAKPIPTSEELHQINKSKIQTKLQYFLETGEIVRRSQRVLNRMAKINTKYRNTKSDGYDLKKGLRTLKERYDTAEFIDKEGNPFPISSLNEYDLKYNSSKARTIFDNLVNIKKITKSRIDQNLMLCLLGTNSEQLKDEFFITKNMLELLTVDNDMERALYLVQIADKSCAAVGMNVLTKWVLDKGKVEQGLRMFSKRSKFGVPTNKHTLVILFDGIAKASEWGQVNNKLADRVIEIFKSSREKSGGTIEEFNACLSVLIKNFDDQQSKAWTFFTNLLPDEENNLREILPNDITFTIMINGLKRFMNHNKELIRAVPEMNRYERALKLMENEANAVQMVDAAFEKIKTLAIQNPGRINLGIQLIVSYLSCYIDKFGSPYRYNEKALYMASQYSTSVNEIFEYVQRAAEIEDVTRVTKSFMKKRDSDIEKLLKQAPQDIKFSHYESPLDFLPKAVVPDLKPENVNPIVNMPIKVPTKKGKFETIYRDRPLVDFAREKDSNKTGTIKLPINKFLLNLVFDGLMNIGRFNEFMFALWYSLIKWNGVKLDLDEIANKHDALKGVIPSDKLPRYSEKELVTEANSSPVVQDVYDDITLKNILLRISTNRGKGETYTHLIVELFNVLSSSKHNPNGIVPSYDHVKLIFFIFREDLKYYKAANINEDKTRGFHFEQLREFNTNLYNFTQGYMLLNQRKQRATHPTIVDELNEIYATIYSNNWNTLTPEQELLIHKLIIKSGILFLPKHSIKISESVGRTMNLLKEKTDISTQDKKLLGKIKDLVKVRKDKNGENVQELEKLTRAVYYAVVIDPVGTVSTVEAMADENAEIIELNSQQQPTNDATEISSSENVVAKPDNTEAEVLSHSNEKSHDSLTTDATDVPKVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM42</accession>
    <name>G3AM42_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_70744</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374262.1">
        <property type="nucleotide sequence ID" value="XM_007374200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM42"/>
    <dbReference type="GeneID" id="18875346"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70744"/>
    <dbReference type="eggNOG" id="ENOG502QT05">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036005_0_0_1"/>
    <dbReference type="InParanoid" id="G3AM42"/>
    <dbReference type="OMA" id="FVDAYPK"/>
    <dbReference type="OrthoDB" id="10257314at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000907">
        <property type="term" value="F:sulfonate dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044273">
        <property type="term" value="P:sulfur compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000003">
        <property type="entry name" value="Alpha-ketoglutarate-dependent taurine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051323">
        <property type="entry name" value="AtsK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468:SF1">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="7">
        <location>
            <begin position="82"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="387" mass="42973" checksum="F2564792DAD1AB58" modified="2011-11-16" version="1">MAPVRYTQGPRGLYAGSKLETTAKGWVVVAPESASKVNKEVHAYLPTWNKDEHYEPYKFHEYHDPALRADPSFPNLLANAETKNISPKLGTEIKGVQLSQLSDAGRDELALLVHQRGVLVFRDQDFVSKGPQHFVDYVKYFGNPHIHPTSGRPQEIANIHVVLSGDTKEDPYNARTNLVGFHSDVSYELQPPGISFLTVTNLPAGGGGDTVFIDSAEAYNRLSPIFREKLEGLKAIHSAVNQANGALSKGSVVRRHPVENAHPIVRTTPLGKKVLFVNRGFTRRIEGLKVEESDAILDFLFDHVAKAHDLQIRANWEKNTVVVFDNRIVNHSAIVDFDTTESRLIARASAQAERPIGDLKDLNKPAESHLFEGPEYLGNKLEDLKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMG8</accession>
    <name>G3AMG8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-isopropylmalate synthase</fullName>
            <ecNumber evidence="5">2.3.3.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LEU6</name>
        <name evidence="13" type="ORF">SPAPADRAFT_60164</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21524"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.13" evidence="5"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374335.1">
        <property type="nucleotide sequence ID" value="XM_007374273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMG8"/>
    <dbReference type="STRING" id="619300.G3AMG8"/>
    <dbReference type="GeneID" id="18873253"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60164"/>
    <dbReference type="eggNOG" id="KOG2367">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004588_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMG8"/>
    <dbReference type="OMA" id="DQIEYMH"/>
    <dbReference type="OrthoDB" id="418791at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07942">
        <property type="entry name" value="DRE_TIM_LeuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000045">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005668">
        <property type="entry name" value="IPM_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054692">
        <property type="entry name" value="LeuA-like_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039371">
        <property type="entry name" value="LeuA_N_DRE-TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00970">
        <property type="entry name" value="leuA_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002991">
        <property type="entry name" value="PRK03739.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46911">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46911:SF1">
        <property type="entry name" value="2-ISOPROPYLMALATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22615">
        <property type="entry name" value="IPMS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="12">
        <location>
            <begin position="33"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW32820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="407" mass="45269" checksum="BB537DF492F31CD2" modified="2011-11-16" version="1" fragment="single">MPMLADPSVKYKKFPDVHLPNRQWPSRSLNKTPRWLSTDLRDGNQSLPDPMSISEKKQYFQKLVEIGFKEIEVAFPSASQIDFDFTRFAVETAPADVSVQVLSPCREELITRTVESLKGAKRATVHIYLATSDCFRNVVFGLSKEESKRKAVECTKLVRKLTKDDPSTAGTDWDFEFSPETFSDTDLDYAVEVCEAVKAAWEPTEEKPIIFNLPATVEMATPNVYADQIEYFATHISNRETVCISLHPHNDRGCSVAAAELGQLAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGISPKLDFSDINSVIDIVEKCNKIPVHARAPYGGSLVVCAFSGSHQDAIKKGFAAHEKEKEKAQGKQVHWKLPYLPLDPQDIGRTYEAIIRVNSQSGKGGSAWVILRNLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMJ1</accession>
    <name>G3AMJ1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_151536</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BUB2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374949.1">
        <property type="nucleotide sequence ID" value="XM_007374887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMJ1"/>
    <dbReference type="FunCoup" id="G3AMJ1">
        <property type="interactions" value="198"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMJ1"/>
    <dbReference type="GeneID" id="18870898"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_151536"/>
    <dbReference type="eggNOG" id="KOG2058">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029367_2_1_1"/>
    <dbReference type="InParanoid" id="G3AMJ1"/>
    <dbReference type="OMA" id="CHKSEPQ"/>
    <dbReference type="OrthoDB" id="10263206at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990334">
        <property type="term" value="C:Bfa1-Bub2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902543">
        <property type="term" value="P:negative regulation of protein localization to mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031030">
        <property type="term" value="P:negative regulation of septation initiation signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000035">
        <property type="entry name" value="Cell cycle arrest protein BUB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000026">
        <property type="entry name" value="Mitotic check point protein (Bub2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF263">
        <property type="entry name" value="MITOTIC CHECK POINT PROTEIN BUB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="6">
        <location>
            <begin position="55"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="322" mass="36714" checksum="031ECEEEE892A2AA" modified="2011-11-16" version="1">MLSQDQQIQQIPAGKARKKDSIEQFIESTQPFLENGLSQLRYMILIEGLSVPPGYDQCPYRAYVWSILSRAQLYPVENYINLLAHAEETLSPELYGKIKNDTFRTLMNDTKFHSKVSEDTLVRVLSCLAITMENKVGYVQGLNVLLAPIAYVCHRSEPQAFSILHQLITFHIPLYITPNLDGVHTGLALIDIVLKIIDPVLSDYLDSKFLKPEIYAFPSVLTLCACTPPLNSVLKLWDFLFAYGTHMNILFIVAQLIINRTALLNSSQPMKLLRNFPNLEPNEIIKLSLSFIPELPKKLYDLISRHGYDPNVPHELKQFKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AML4</accession>
    <name>G3AML4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LCCL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_55337</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374973.1">
        <property type="nucleotide sequence ID" value="XM_007374911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AML4"/>
    <dbReference type="FunCoup" id="G3AML4">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AML4"/>
    <dbReference type="GeneID" id="18871929"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55337"/>
    <dbReference type="eggNOG" id="ENOG502QUEX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011125_2_0_1"/>
    <dbReference type="InParanoid" id="G3AML4"/>
    <dbReference type="OMA" id="HWDKTVL"/>
    <dbReference type="OrthoDB" id="441660at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.20">
        <property type="entry name" value="LCCL-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051957">
        <property type="entry name" value="CRISP-LCCL_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004043">
        <property type="entry name" value="LCCL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036609">
        <property type="entry name" value="LCCL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31331:SF1">
        <property type="entry name" value="CYSTEINE RICH SECRETORY PROTEIN LCCL DOMAIN CONTAINING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31331">
        <property type="entry name" value="LCCL DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G08630)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03815">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69848">
        <property type="entry name" value="LCCL domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="262"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="428"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="472"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="LCCL" evidence="2">
        <location>
            <begin position="120"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="638" mass="71921" checksum="23FEF5CAF82CD55E" modified="2011-11-16" version="1">MVAKRQRGIGAKISRFFRALWQGPWEPRDDPAPQIACLQRLEEIPEVLNKRYPANIRIAGLVFYLMFWFGLFYSIVMPYLSVPPHLSNDPEVHIIPLSCTSSSQFWKGKNGACGTDGEFCPEFKSDEVIFRCPALCDRAWTYSLIPIGDQRIKYRGYFIGGGDQVTEKVEDDDDQLTNPYRADSYPCGSGVHAGVISPFFGGCARISYKSKEREFFKSEKGHYGVDDSIEFLSFFKYSYYFKQLMSGVLPGGSFQQCYDPRLLVLVMNFVLGIPVVYLSSGAVSYWITSIVGFWTHTLATDPPVHVDPYDPDTLSDLISVSLERFLPTCFILYVLWHFSSRRTLFDSTEPDEKSSPLTKVFLWYPLFWLGLLNNMTFDRLPVDRLTLSDLKEQPGSILALAFIISCIGTGVVIQAYKIWLSGRFRKYLLVYALFIFGLVVLAQLPGLTLRVHHYILAMLLIPGCSTRGRTAFMFQGILLGLFLSGAARWGLAAIAETVTSLKRDDPRGILVPPEFLGYNNETGILNWKGVPEELLSPITSRLYEKYSAISLLVNDIEQYVGEKTQSVNITELFTNSTSLSRPIAKSLRDGIKDDNGNIEIYLRIGRKIPNTQVYSDFSNAGILKWPSGDFTLPVKGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMU0</accession>
    <name>G3AMU0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Drug resistance protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_50407</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375049.1">
        <property type="nucleotide sequence ID" value="XM_007374987.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AMU0">
        <property type="interactions" value="399"/>
    </dbReference>
    <dbReference type="GeneID" id="18871439"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50407"/>
    <dbReference type="eggNOG" id="KOG0065">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_35_0_1"/>
    <dbReference type="InParanoid" id="G3AMU0"/>
    <dbReference type="OMA" id="RWANPIY"/>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005285">
        <property type="entry name" value="Drug-R_PDR/CDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00956">
        <property type="entry name" value="3a01205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="499"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="533"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="575"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="611"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="641"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="747"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1253"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1299"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1333"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1449"/>
            <end position="1470"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="137"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="840"/>
            <end position="1084"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1496" mass="169254" checksum="EAB6F063B39B0C5B" modified="2011-11-16" version="1">MSNQIDLPDSGSFDKPYESYVGFDEEASKDVKQLAKTITQESQSSSAALIRYLSNMDEVPGINPYEQEIADPKLDPESPEFNAKYWIKNMKALYQSDPDYFKPNKLGIAFRNLRAYGIANDVDYQTTVATGFLKFVVDGLRHFRKEDESRYFDILKPMDGLLLPGELTVVLGRPGAGCSTLLKTLAVNTYGFNIDKESKISYDGLSPNDIEKHYRGDVIYSAETDEHFPQLTVGDTLEFAARLRTPQNRGLGVDRETYAKHMASVYMATYGILRQRNTPVGNEFVRGCSGGERKRVSIAEVSLSGANIQCWDNATRGLDAATALEFIRALKTSSSILDVTPVIAIYQCSQDAYDLFDNVSVLYDGYQIFFGKASKAKEFFINMGWHCPARQTTADFLTSLTNPAERIAAPGYEGKLPRTAQEFETYWKKSPEYQKLVADIDQYFLDCEKLNTKKAHHESHVARQSNHISPRSPYTVSFPMQVKYIMSRNFLRMKGDPSIPIFMVVGQVIMGLILSSIFFNLDQTTKSINFRGIALFFAVLFNAFASILEIMSLFEARNIVEKHKKYALYRPSADALAGIVTELPTKLFMSMAFNFTFYFMINLRRDPGHFFYYWLMAITCTLVMSHLFRTIGAMATSFAQAMTPAAVLLLAMVIYTGFVITTREMLGWARWINYINPVGYVFESLMVNEFHGRYFECSNFVPSGGSYDTISAANRVCTAIGAQPGQLMVSGTTYLKMAYDYQNAHKWRNFGICIGYIIFFLLTYIIVTEFNKGAMQKGEIALFLRSSLKQLKNRKELAAKNQDIENYTNEKVPLGDLLASEKKDNGSSSDNSLTTEKEIFFWRNLTYGIKIKKEERTILDHVDGWVKPGEITALMGATGAGKTTLLNCLSERLTVGVITDGVRMVNGHSLDSSFQRSIGYVQQQDLHLPTSTVREALKFSAYLRQSNTTPDKEKDAYVDYIISLLEMEEYADAMVGVAGSGLNVEQRKRLTIGVELVAKPKLLLFLDEPTSGLDSQTAWSICKLMRKLADHGQAILCTIHQPSALLLTGFDRLLFLQAGGQTVYFGDLGENCQTLINYFQKYGADPCSKDANPAEWMLQVVGAAPGSHAKEDYFQVWRNSEEYQEIQQELDTMEVKLAKLPRNTDPESHLKYAAPWWKQYWMVTARAIVQDWRTPSYIYSKLFLCLATSLFNGFSFFGGHLSVRGLQNQMFAIFMFYVPFNTMHEQTLPYYLAQRDVYEVRERPSRTFSWFAFIFGQLTSEIPYQIMVGTLAFFCWYYPSGLYKNAQAADEEASRGVLMWLLVVSFYCYTSTMAHFCISFLERQENGASTGHLLFQLCLLFCGVLAGPSQLPGFWIFMYRFNPITYLTQGILAVGLANNKVVCAPQEYVSINPPDGTSCDDFMAEYIQKAGGYVLTDDATGQCQFCEIDSTNEFLASVGAHFSQRWRNYGVFVAFIGFNVILAIFLYWLARVPKGNRERKKNSKDERLKEGKRGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN12</accession>
    <name>G3AN12_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AP complex subunit sigma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_138919</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375702.1">
        <property type="nucleotide sequence ID" value="XM_007375640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN12"/>
    <dbReference type="FunCoup" id="G3AN12">
        <property type="interactions" value="471"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN12"/>
    <dbReference type="GeneID" id="18870161"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138919"/>
    <dbReference type="eggNOG" id="KOG0935">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061221_3_0_1"/>
    <dbReference type="InParanoid" id="G3AN12"/>
    <dbReference type="OMA" id="QSNFVEY"/>
    <dbReference type="OrthoDB" id="371463at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000010">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000804">
        <property type="entry name" value="Clathrin_sm-chain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015588">
        <property type="entry name" value="AP_complex_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00989">
        <property type="entry name" value="CLAT_ADAPTOR_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="7">
        <location>
            <begin position="3"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="145" mass="17450" checksum="5CABAF97CAD0F28E" modified="2011-11-16" version="1">MAIHFLLILNRQGKTRLVKWFNNTYTTQTKQQYIQEIHRLINSRDSKHQSNFVEFYQNKLVYRRYAGLYFVCLVDLNDSELSYLESLHFLVEILDDYFNNVCEVDLVFNFYKLYFIIDELYLGGEIQQISKSKILNRLAVLDKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
    </protein>
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    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375190.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN71"/>
    <dbReference type="GeneID" id="18875106"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66599"/>
    <dbReference type="HOGENOM" id="CLU_746327_0_0_1"/>
    <dbReference type="InParanoid" id="G3AN71"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="371" mass="43639" checksum="7AB947F80A5AF5E3" modified="2011-11-16" version="1">MSLMLTNKRMYHVAVKRLYQLIYVEMGILINTDICTSFNCKYTVTQCGLKLLNQRNELLQHVKLIVVQNYETYNYYITLWNAKKYDWIQLCPEKLYLGYHLHSYDRIEIAFLALIIKYFLPSPPVLENDDYTITSIVIPLCDTFVDELDLSIIPYLKRLQRIKFQMVWGNELTLIKSKFSHCLNITCLSLQFSLLNHPEFECLIDIINLDAVTSLELFIDNVLHVVVQQLYFDWLIAHLPNLNSFLVRSNMAKNIHNVCSLIMENTLSRLVILSEDFTPSEVWEQVKKQEQTIEQLFIGRCNIDFTLNYLDRIFKVTEKDNDRRLTYESMIAGFLGDDTSRYPQLSNIIVYGCDYEISRSNSSFKVNPLNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANT7</accession>
    <name>G3ANT7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_50622</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375298.1">
        <property type="nucleotide sequence ID" value="XM_007375236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANT7"/>
    <dbReference type="GeneID" id="18871463"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50622"/>
    <dbReference type="HOGENOM" id="CLU_1928912_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANT7"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442464" evidence="2">
        <location>
            <begin position="24"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="131" mass="14818" checksum="581D1B09D7CA1E8D" modified="2011-11-16" version="1">MDFRDLLLIILAIFTWIGGVAHAAFVVVSNPHIKPEIIVVSPTNHGSNSDPYYKTESYSSGYPNPGSNPPQNPPGYNAQLDHPKHENGFYDAIEAKEENQESFKFYLDPLDENNIKLERNIITKIIYNNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANV4</accession>
    <name>G3ANV4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_61654</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375855.1">
        <property type="nucleotide sequence ID" value="XM_007375793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANV4"/>
    <dbReference type="STRING" id="619300.G3ANV4"/>
    <dbReference type="GeneID" id="18873989"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61654"/>
    <dbReference type="eggNOG" id="KOG1291">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020088_4_0_1"/>
    <dbReference type="InParanoid" id="G3ANV4"/>
    <dbReference type="OrthoDB" id="409173at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015086">
        <property type="term" value="F:cadmium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF50">
        <property type="entry name" value="MANGANESE TRANSPORTER SMF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00447">
        <property type="entry name" value="NATRESASSCMP"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="432"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="155"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="461" mass="50814" checksum="CC453375B2C2E14A" modified="2011-11-16" version="1">MGATVMPHSLYLGSGLVQARLKDFDVKNGLFKPLKVGFEPSEQTRPDSIVSSSTEVNGEDEYDLTPLATPYIQDQYEKEDESTLNVEEDDDEGYRPSVQAIDHTMSYTIIELVISLFTVALFVNAAILIVAGATLDSDKIPGHSMFRRDEHSDGNDSDSDSDSDGENTDYGNADLFTIYHLLSRHLSPTAGFVFALALLCSGQSAGVVCTLAGQMVSEGFLNWSMSPVVRRLITRALAIAPCLIVVSMSGRDGLARTLNASQVVLSFLLPVVTAPLIYFTCSKEIMRVPVYTKDGDEDVIFEAGTVDEERVENRPVALKQTPQKRFKSSDSAILLQDLRNSHPCAGWEDDELDHEHQQHDEERRLIETAASEAAYSQSHSMMKTGSVSIPVTTTISNDNNNTSLPIEIFENDDHEVYRIQGYKDFSNGPTTAFLAVLIWGFITVLNLYLIGSLFLGYDVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP46</accession>
    <name>G3AP46_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_50672</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375353.1">
        <property type="nucleotide sequence ID" value="XM_007375291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP46"/>
    <dbReference type="GeneID" id="18871472"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50672"/>
    <dbReference type="eggNOG" id="ENOG502T3GY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_585391_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP46"/>
    <dbReference type="OMA" id="YKEPSFY"/>
    <dbReference type="OrthoDB" id="4078100at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024274">
        <property type="entry name" value="APC9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12856">
        <property type="entry name" value="ANAPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="457" mass="53192" checksum="5CF29CA9D1FBCDD0" modified="2011-11-16" version="1">MNTSTTNNNSNNTNNNNSTSRISTNRYLNLPKFTILHPPNPRISSSNSTTSPNDSHNITNRTISSSSSIMSTPSNNIKRNRWKQQQQQQQQQQQQQQKEQKQQNKLDKQKKGISASKTGRLVKSKLLNLHQSSKLVFDDKSLPKISHKSKTIGSKQEPGPYDYSIFTHYRNGNGDVAPATRESQIRAWESSERIAQSVIFPSSDSEVDDLEEEEEEEENQEEGVVECPIVSIPGYTVGETKIIYHAYTHPKVDEERRLMEHRHKVMRKMDKEAASSFMATNSLLTKWKLQVSQKKEIYSRIFGQDNLMNGEYMTNNATYSSVDNPRNLQKTLAEDKLEITNLLAEEKEHNLIQHEIRHQFNMHVLNDFELKKKRRKNSNGSSSSEDHIDYDRFQLLTKKKLDKIYQDNIVEYEDIIQEELDRDDDDEEEEEVMEQELLNFTTSYLRKRVKEVEHSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP70</accession>
    <name>G3AP70_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61701</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375917.1">
        <property type="nucleotide sequence ID" value="XM_007375855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP70"/>
    <dbReference type="GeneID" id="18874012"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61701"/>
    <dbReference type="eggNOG" id="ENOG502RQ27">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_520729_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP70"/>
    <dbReference type="OMA" id="PILGHAY"/>
    <dbReference type="OrthoDB" id="4096810at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="435" mass="49960" checksum="899ED58186673546" modified="2011-11-16" version="1">MSQVRIQRLPEIPRSKSARSDYTLQFQDDRGRAQTRKSHHARLRMNDASAIPRSESTRRPQVRRTHSVNQNHAVRSSRSVSEHTFNKNIPMEEERRSRPQVKKWNSFHGPDREMMIPRDSSVFSVIEDRYDTRQLPHYSWNSNVPNSSTDNLFKALEEPSPTSEIEIPEEAPRGTSLYEKMSKLIIRAQNSVGSLVPLSLKNPQSGSASDTRSESSDSSDSEEEVVRANNTDTQYTIESWKSPPALQPVNTDSLAQILDEINEFHGNFVDKNVNHSDVIGLNRTQQRILDYKQLYEYEEVSKPGLSSYELKIQNETILSQYTSIRLRFSSHEIMSSKKHMVKTDTGVLGFVNRAKNFGLGSENKNNEVTLENKDMFLQKIWDDEYYNCFVDTNHETIEEKTNDDVKSISSEVGIYNQDKLVDMSNLAKSVKFGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3APC1</accession>
    <name>G3APC1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Spliceosomal protein DIB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_139739</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">Essential role in pre-mRNA splicing. Also essential for entry into mitosis (G2/M progression) as well as for chromosome segregation during mitosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DIM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375374.1">
        <property type="nucleotide sequence ID" value="XM_007375312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APC1"/>
    <dbReference type="FunCoup" id="G3APC1">
        <property type="interactions" value="919"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APC1"/>
    <dbReference type="GeneID" id="18870234"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139739"/>
    <dbReference type="eggNOG" id="KOG3414">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117348_0_0_1"/>
    <dbReference type="InParanoid" id="G3APC1"/>
    <dbReference type="OMA" id="GMYELYD"/>
    <dbReference type="OrthoDB" id="147752at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02954">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000004">
        <property type="entry name" value="Spliceosomal protein DIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004123">
        <property type="entry name" value="Dim1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052:SF5">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEN 4A, 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02966">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017199">
        <property type="entry name" value="mRNA_splic_U5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01410">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="148" mass="17230" checksum="7A201BAD3A6AB96C" modified="2011-11-16" version="1">MGSVFLPHLNTGWHVDQAILSEDDRLVVIRFGREEEPDCMIIDEILFSISEKVKNFAAIYLCNLDKVPDFNQMYELDQDRSEPYTIMFFFRNKHMMCDFGTGNNNKLNFMIYDKQELIDIIETIYRGAKKGKGLVVSPKDYSTLRRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3APH7</accession>
    <name>G3APH7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_138659</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375425.1">
        <property type="nucleotide sequence ID" value="XM_007375363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APH7"/>
    <dbReference type="FunCoup" id="G3APH7">
        <property type="interactions" value="163"/>
    </dbReference>
    <dbReference type="GeneID" id="18870138"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138659"/>
    <dbReference type="eggNOG" id="ENOG502QTZH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025894_2_0_1"/>
    <dbReference type="InParanoid" id="G3APH7"/>
    <dbReference type="OMA" id="LGYGFMP"/>
    <dbReference type="OrthoDB" id="10027823at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520:SF2">
        <property type="entry name" value="ABR173CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="327"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="404"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="3">
        <location>
            <begin position="13"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="444" mass="49424" checksum="D16BF28950F4D741" modified="2011-11-16" version="1">MHLLKSLRQSNSDIRLLWSSVFFRMASYGLTNQVLTLYLKAIDISEERIGIFMTLTLIGDTLISYYLTWNADKIGRRVVMIIGTVMMFGSGITFAISSDFYVLLAAAIVGVISPSGDETGPFKSVEEASIAHLTPHNHRPEIFAFHGLFATAGAALGSLVCGVLVDYMNQAWGYEMEQCYRYIFVLYAGFSLIKLTLMLFLSNKCEVYVDNFIEEQDKDSDEESPLLVDATPDEEISSIKSGLSNTTRYYLPRLLAIFMLDSLGYGFMPSAWVVYYLKITLLLSASALGTLFFFTNSINAISSLPSAYLAKLLGPVKAILFTQVPSAIFFGMVPIFNTYLPVAGLLMLYYTTTTMDVVPRQVLLTSIMPKEEITKVMGIVNIGKTFARCIGPIFTGKLAAHNKLYIGFFINSICVLLADLIMATNFLHLDSEILQKQSIEHRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3APN7</accession>
    <name>G3APN7_SPAPN</name>
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        <recommendedName>
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        <name evidence="6" type="ORF">SPAPADRAFT_139594</name>
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        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Saccharomycotina</taxon>
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        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007375422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APN7"/>
    <dbReference type="FunCoup" id="G3APN7">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APN7"/>
    <dbReference type="GeneID" id="18870219"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139594"/>
    <dbReference type="eggNOG" id="ENOG502RQI2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029376_0_0_1"/>
    <dbReference type="InParanoid" id="G3APN7"/>
    <dbReference type="OMA" id="ELWRGWR"/>
    <dbReference type="OrthoDB" id="77989at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="217"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EGW32208.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="528" mass="59957" checksum="03000541F509D2BE" modified="2011-11-16" version="1">MSSQVNASQLRPYYDHDSFDAGYSVIFKTGVGLVDTQTNKPITAKFSASAIDRAINKGGSGSGVLSKSLGRTGGGIGIGFSGDSHGGAGMFKDKNYIYDLEFNEYFDLHNLAELFKNLFWNFIKNYTKVLLSQPLEIVRLVLQVGKFEFVSPEKKLTPDLSESRRLLAEDMGEFSSPAPYTTEEEDDDVIDYFQSNIEQSVWSNEQSRQSLDTSEVRKKKKSKRRSGSRINKIQPKSIHTVDIMSAIVNKDGPFALFRGINASFIYQTLSHTIEAWITGFISPFLGIPDPFFLDLTHSNDPFKSLWLSVSACVLTGLILVPLDLIRVRLMVTQFIGDLKEQEDEITQLVEQTIQSTRSVRESIRNFPVYYLSHPPTPVVFLTILHQFSISIFRKIAPYILFIKFNIDSYSSPNIYTFVNLISLIMEFFIKLPVENLLRKEQVRFLLTSKPEDVMKVITIENPQENLIVEFNGKVNEAEQDEDTPSTFLERVKQMGLFNGWRVGVLNVIGFWGYNILKNDGTELKEERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3APW8</accession>
    <name>G3APW8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_153012</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007375503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APW8"/>
    <dbReference type="FunCoup" id="G3APW8">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APW8"/>
    <dbReference type="GeneID" id="18870939"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_153012"/>
    <dbReference type="eggNOG" id="KOG3780">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005724_0_0_1"/>
    <dbReference type="InParanoid" id="G3APW8"/>
    <dbReference type="OMA" id="NMELPTY"/>
    <dbReference type="OrthoDB" id="2333384at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="465"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="829"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="887"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="920"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="85"/>
        </location>
    </feature>
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        <location>
            <begin position="86"/>
            <end position="101"/>
        </location>
    </feature>
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        <location>
            <begin position="209"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="833"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="857"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="922"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="937"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="968"/>
            <end position="980"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1020" mass="113919" checksum="F491DA1409B7B02C" modified="2011-11-16" version="1">MFKIPLGTSAFSDTNNAVTSNNNTLQPTFVQTMHPRSFSFDRIKSKVTGRHDATSETNTNSNRRNSMATVSHQPQQQQQQQQQNQHLASQPSPTRRAQSISFMPPPTRSRSRSISKTNQPTLHLNPFKSTTKELVKQETAQIISKKLVNMLSDLGLQYPIPLRTTTSGSPSKSIKIYVANTNDCLYLPPASSTSFTYEDVENGGAVPILEDDDDDDDDDNVFGSRASVDSPVLGTSEPEQQPTLHSKMKSFKSPNYLCSKIDSEHPIPHTFAVVIELTKESTTIKDVTFEFQSLTQILWPNGDLYNKAFAKESFKIGSLQWKTSLSEADYYINNLNSNEVKSKNITPEDLAKRTREYHLVDIRELDSAGLKEYNERASSSSHGDSYKAGLYVFLLPILLPEHIPASIISINGTLAHTLSVNFNKISERLNRKIKVNASYNLPMVRTPPNFANSIADKPIYVNRVWNDAVHYIITFPKKYVSLGNEHVINVKLVPLVKDVIIKRIKFNVLERITYLSRDLRREYDYDSENPFLAHAPSDKVRERVISLCELRTKNKSSSNGFTDPFKEEVIKCPDNNLLYTCYEPEDKIGESPPASPGKHRKHSHVTTQRAMIASPLDINIALPFLTSRTDKLMMTASSQDDEPDKPRSRRASLIMDGFESTKESSVIGTLETNLINHDEVGPHELIKPSSSTYVSDDLSVKHRPPENIQKGFTILSRALYPDSNYRHIQINHRLQVCFRISKPDPKDNYKMHHYEVVVDTPLILLSSKCNDGSMQLPKYDEIDIADIVAEPASPKISFRTPNYQHNGVTIKQWDQDVTHEEYLPTFEEAVKQPSGSSTTPSPSPVVRSMSIEEDPLSRIPSISLPPGVNSDPPAYEPPSDKLIDEVVSSSSEGDGMPGPSRMKASLSNSFAKKIELEDDGSIDSNLSSHSSDGSSFIVGTSESSVQDTPDLSASLSAPPAINVSSPEEGNEEEEEEEEESSVVYLNENNASKVMNKAISEFAIDDDDERRDEIITSVDNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3APY8</accession>
    <name>G3APY8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_61387</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375584.1">
        <property type="nucleotide sequence ID" value="XM_007375522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APY8"/>
    <dbReference type="FunCoup" id="G3APY8">
        <property type="interactions" value="751"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APY8"/>
    <dbReference type="GeneID" id="18873868"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61387"/>
    <dbReference type="eggNOG" id="KOG0767">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039456_1_0_1"/>
    <dbReference type="InParanoid" id="G3APY8"/>
    <dbReference type="OMA" id="KMYIEEG"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000024">
        <property type="entry name" value="MIR1p Mitochondrial phosphate carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF12">
        <property type="entry name" value="MITOCHONDRIAL PHOSPHATE CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 13" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="80"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="179"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW32308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="273" mass="29071" checksum="60BC4427483ED62B" modified="2011-11-16" version="1">MCPIDVVKTRIQLEPTVYNKGMLGSFKQVVANEGAGALLTGLGPTILGYSLQGAFKFGGYELFKKNFIELLGYDTAKQYKNSIYIGSAALAEFFADIALCPLEATRIRLVSQPTFADGLIGGFGRILREEGVGSFYNGFTPILFKQIPYNIAKFLVFERAAEAIYAAIPTPKKDLSNSTNTLVNLGSGIIAGCAAAIVSQPADTLLSKVNKTKKAPGQSTVGLLVQLAKQLGIKGSFTGLPTRLIMVGTLTSLQFTIYGTIKKALGCPPAVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ09</accession>
    <name>G3AQ09_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_139497</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="15"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375606.1">
        <property type="nucleotide sequence ID" value="XM_007375544.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AQ09">
        <property type="interactions" value="1121"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ09"/>
    <dbReference type="GeneID" id="18870213"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139497"/>
    <dbReference type="eggNOG" id="KOG0262">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000487_2_4_1"/>
    <dbReference type="InParanoid" id="G3AQ09"/>
    <dbReference type="OMA" id="NREDYQQ"/>
    <dbReference type="OrthoDB" id="270392at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02735">
        <property type="entry name" value="RNAP_I_Rpa1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01435">
        <property type="entry name" value="RNAP_I_RPA1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.30:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.390:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.100:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.120:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.180:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.120:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047107">
        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015699">
        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF11">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15 18" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="17">
        <location>
            <begin position="323"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1307"/>
            <end position="1420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="262"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1377"/>
            <end position="1399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1400"/>
            <end position="1420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00053996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW32330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1639" mass="183800" checksum="76F786AFEC7D067A" modified="2011-11-16" version="1">MDISKPVGSEIKSVDFGVLSDNEIKRLSAKQITNPTVFDNLGHPINSGLYDLALGAFLRNVCTTCGLDEKFCPGHMGHIELPVPVYNPLFFNQLYILLRASCLYCHKFKLNALEVHKYQCKLRLIQYGLLLECVDLENMLAKGAQEIEDDDDDNDTLDQKTKQELMERRELFVNTAIAKALSEGRTSERGVVTATVGEERKDTIHDFYKRLLSRPKCDNCGMYSPTFRKDGFTKIFENALTDKQVSNNRVKGLQRPDMIRNTKGNSNTSSGDNLPNIRHKGGSKYVLSSEIRNIIRAVFTNEQPIIQKVFHSRPYQKEIVKGDIFFRQCILVPPTRFRLPSKLGDEIHENSQNELLSNILKTSLLIRDLNDQISNMYQEKLSGEQKKIIFNRLMNAFVTIQNDVNAFIDSTKNQNAPAGKVPNPGIKQALEKKEGLFRKHMMGKRVNYAARSVISPDPNLETNEIGVPPVFAKKLTYPEPVTSYNASELRQAVINGPDKWPGAVQVQNEDGSLVSLIGMSLEQRKAIANQLLTPSNGASVVNKKVYRHIKNKDVVIMNRQPTLHKASMMGHKVRVLPGEKTLRLHYANTGAYNADFDGDEMNMHFPQNENAKAEALNLANTDNQYLTPTSGSPLRGLIQDHISAGVWLTSKDTFFTREIYQQLVYGCIRPEDGHTTRNRIITVPPAVFKPQQLWTGKQVITTILLNIKPDGVPGVNLISKNKIKSDYWGAGSTENDVIFKNGELLCGILDKSQYGASQYGIVHSLHEVYGPTVAGKALSVLGRLFTNYIMMTAFTCGMDDLRLTNEGNEWRKEILKQSVDIGRVAAAEVTNLDQDTSNHDPELLRRLEEILRDDNKLGILDAITQSKVNTITSQVVSKCVPDGTMKRFPYNSMQAMALSGAKGSNVNVSQIMCLLGQQALEGRRVPVMVSGKTLPSFKPFETDARAGGYIKGRFYSGIRPQEYYFHCMAGREGLIDTAVKTSRSGYLQRCLTKQLEGVHVSYDNSVRDGDGTLIQFLYGGDAIDTTKQSHMNQFKFCADNYDALLAKYNPGDMTDNLDVEEALSYSKKVRKSLKKQKELPHYEQQIKYDPVMNVYNPAKYLGSVSEKFQDKLDAFVSENSQIFAKNKEEAKSTGKLTEKKFRALMQLKYMRSLINPGESVGIIASQSIGEPSTQMTLNTFHFAGHGAANVTLGIPRMREIIMTASAAIKTPQMTLPILDDVTDDQADAFCKSVARVVLSEFIDRVTVTETTSQSEDGTNSRSYVIHLKFYSKQEYEQEYDVEQEQLEDVITTNFLHALEQQIVKEVKKQKKPDHLPTVGKSAGKTDMESVSGRVMDGTTNDDEDAEEEKMRGNVKQAVSYDGPDDEEIETMKKAEETSDEEMEDSDSDDSSSDEEEEDVDMKPETAKKSNELSRSAKDRQSEVIASHNMVTKFNFDDDEGEWCEFQLELNGNETQKLLMVNIVEDLCRRVVVREIPRIGRCIRPQPDAKGNRLLTTEGVNFKAMWDQDDFINVNNITSNDVAAVLHTYGVEAARNTIVNEIYRVFDTYGISVSSRHLDLIADMMTREGTYLAFNRQGIDSGTSAFMKMSYETTCQFLTKAVLDGNREELESPSARIVLGKLSNVGTGSFDVFAQIPKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQB0</accession>
    <name>G3AQB0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">60S ribosomal protein L12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_62028</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376235.1">
        <property type="nucleotide sequence ID" value="XM_007376173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQB0"/>
    <dbReference type="FunCoup" id="G3AQB0">
        <property type="interactions" value="1054"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQB0"/>
    <dbReference type="GeneID" id="18874156"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62028"/>
    <dbReference type="eggNOG" id="KOG0886">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074237_5_0_1"/>
    <dbReference type="InParanoid" id="G3AQB0"/>
    <dbReference type="OMA" id="QPPHDVI"/>
    <dbReference type="OrthoDB" id="1478556at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.250:FF:000002">
        <property type="entry name" value="60S ribosomal protein L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1550.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_uL11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="7">
        <location>
            <begin position="14"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="6">
        <location>
            <begin position="74"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="165" mass="17711" checksum="5137C76AD41ABCAF" modified="2011-11-16" version="1">MPPKFDPSEVKFLYLRAVGGEVGASSALAPKIGPLGLSPKKVGEDIAKATKDFKGIKVTVQLRIQNRQATASVVPSASSLVITALKEPVRDRKKEKNVKHSGNIPLEEIYDIARQMREKSFGKTLASVAKEILGTAQSVGCRVNGQNPHDIIDAINAGEIDVPEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQC4</accession>
    <name>G3AQC4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1748-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62041</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376249.1">
        <property type="nucleotide sequence ID" value="XM_007376187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQC4"/>
    <dbReference type="FunCoup" id="G3AQC4">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="GeneID" id="18874163"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62041"/>
    <dbReference type="eggNOG" id="ENOG502S6Z8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151392_1_1_1"/>
    <dbReference type="InParanoid" id="G3AQC4"/>
    <dbReference type="OMA" id="NKEVGKW"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013726">
        <property type="entry name" value="Mitofissin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28075">
        <property type="entry name" value="CHROMOSOME 16, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28075:SF1">
        <property type="entry name" value="DUF1748-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08520">
        <property type="entry name" value="Mitofissin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="71" mass="7995" checksum="35E937B170EE43FA" modified="2011-11-16" version="1">MLGKVAHIGFDVILLSGFLAGVKRSTGITPNLESIENKDVKYYSQKYLDLGERAFDWSVAYLGSSEYFTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQE9</accession>
    <name>G3AQE9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Serine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Seryl-tRNA synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Seryl-tRNA(Ser) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_62069</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction: serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="3"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376274.1">
        <property type="nucleotide sequence ID" value="XM_007376212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQE9"/>
    <dbReference type="FunCoup" id="G3AQE9">
        <property type="interactions" value="831"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQE9"/>
    <dbReference type="GeneID" id="18874177"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62069"/>
    <dbReference type="eggNOG" id="KOG2509">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023797_0_1_1"/>
    <dbReference type="InParanoid" id="G3AQE9"/>
    <dbReference type="OMA" id="GYTPCFR"/>
    <dbReference type="OrthoDB" id="10264585at2759"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110004">
        <property type="term" value="P:positive regulation of tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.40:FF:000003">
        <property type="entry name" value="Serine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000026">
        <property type="entry name" value="Seryl-tRNA synthetase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="entry name" value="Serine-tRNA synthetase, tRNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="15">
        <location>
            <begin position="152"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="278"/>
            <end position="280"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="294"/>
            <end position="297"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="365"/>
            <end position="368"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="13">
        <location>
            <position position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW31496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="461" mass="52679" checksum="0BFE02A4DE069D30" modified="2011-11-16" version="1">MLDINAFLVEKGGNPDKIKESQKKRGASVELVDEIINGYTEWTKIRFDLDEQNKKLNAIQKEIGKKFKAKEDASELVAEKEKLAAGKKDLIEKEAQADSALRFKVNQVGNIVHDSVVVSMDEDNNELVRTWIPEGYEVAQVGAASGKPAKLSHHEVLLRLDGYDPERGVRIVGHRGYFLRNWGVFLNQALINYGLHFLSSNDYVPLQAPVMMNKEVMAKTAQLSQFDEELYKVLDGEDEKYLIATSEQPISAYHAGEWFESPQEQLPVRYAGYSSCFRREAGSHGKDAWGIFRVHAFEKIEQFVLTEPEKSWQEFDRMIGLSEQFYQSLKLPYRVVGIVSGELNNAAAKKYDLEAWFPFQKEYKELVSCSNCTDYQSRNLEIRCGIKQQNQTEKKYVHCLNSTLTATERTLCCILENYQTEDGLIVPEVLRKYIPGEPEFIEFAKELPKNSTSNKKKDGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQV6</accession>
    <name>G3AQV6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Flo11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_72045</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375963.1">
        <property type="nucleotide sequence ID" value="XM_007375901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQV6"/>
    <dbReference type="STRING" id="619300.G3AQV6"/>
    <dbReference type="GeneID" id="18875410"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72045"/>
    <dbReference type="eggNOG" id="KOG1216">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029456_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQV6"/>
    <dbReference type="OrthoDB" id="4096612at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025928">
        <property type="entry name" value="Flocculin_t3_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13928">
        <property type="entry name" value="Flocculin_t3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442519" description="Flo11 domain-containing protein" evidence="5">
        <location>
            <begin position="19"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW31185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="339" mass="34889" checksum="C618246BB28D3AC1" modified="2011-11-16" version="1">MRFTSIIATAVLLTQAIALDVACLVSGVRVAVVDLDTGICPFTIPSGLPILFDFVSPQDYNIIFYYSIAAGERYFNDIVHAGNIISIPAKILFNTPGAPLYQVRAQKEPASNSTEALRRRLWKDTVIEKRDDASDFAETLKDLDGTLVGPDVFQVVPVDEATCTEEITTEVCTVVNVVTCTKTVCEPTTVPAKPTCITTTVNEVVTVLTTYCPESSIAVPTFIVTITEAETVITCPATPALTTVTSAGVTTVVVTTVPVTQTVTKPAAPGVATATTPAAPAGSAVATASTPGAPGASGAATVAPAGSTTNAVVSTFGNGAASVGSTFLALALIPLGYLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQX2</accession>
    <name>G3AQX2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">26S proteasome regulatory subunit 4 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_61781</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375979.1">
        <property type="nucleotide sequence ID" value="XM_007375917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQX2"/>
    <dbReference type="FunCoup" id="G3AQX2">
        <property type="interactions" value="1017"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQX2"/>
    <dbReference type="GeneID" id="18874045"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61781"/>
    <dbReference type="eggNOG" id="KOG0726">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_2_3_1"/>
    <dbReference type="InParanoid" id="G3AQX2"/>
    <dbReference type="OMA" id="QDDTDPM"/>
    <dbReference type="OrthoDB" id="10255768at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036402">
        <property type="term" value="F:proteasome-activating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031503">
        <property type="term" value="P:protein-containing complex localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000030">
        <property type="entry name" value="26S protease regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000007">
        <property type="entry name" value="26S proteasome regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000039">
        <property type="entry name" value="26S proteasome regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="218"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="13"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="440" mass="49024" checksum="45A0E54E148FD26F" modified="2011-11-16" version="1">MGQGPSGMPGDNLNKRKDDKKKEKPKYEPPVESKFGKKRRKGPDTAVKLPSVYPNTRCKLKLLKLERIKDHLLLEEEFVTNQEAFQPTEARQAEEREKVDELRGYPMAVGTLEEIIDDDHAIVSSTASSEFYVSIMSFVDKGLLEPGCSVLLHHKTVAVVGVLQDDADPMVSVMKLDKSPTESYADIGGLESQIQEIKESVELPLTHPELYEEMGIKPPKGVILYGAPGTGKTLLAKAVANQTSATFLRIVGSELIQKYLGDGPRLCRQIFQIAAEHAPSIVFIDEIDAIGTKRYESTSGGEREIQRTMLELLNQLDGFDDRGDIKVIMATNKIESLDPALIRPGRIDRKILFENPDANTKRKILNIHTSKMSLADDVNLDELVSAKDELSGADIKAICTEAGLLALRERRMQVKADDFRQAKERVLKNKVEENLEGLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQX7</accession>
    <name>G3AQX7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61789</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375984.1">
        <property type="nucleotide sequence ID" value="XM_007375922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQX7"/>
    <dbReference type="STRING" id="619300.G3AQX7"/>
    <dbReference type="GeneID" id="18874047"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61789"/>
    <dbReference type="eggNOG" id="ENOG502QQ4S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024075_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQX7"/>
    <dbReference type="OrthoDB" id="4055624at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018843">
        <property type="entry name" value="Utp8_b-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053881">
        <property type="entry name" value="Utp8_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10395">
        <property type="entry name" value="Utp8_b_propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22542">
        <property type="entry name" value="Utp8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Utp8 beta-propeller" evidence="1">
        <location>
            <begin position="2"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Utp8 C-terminal" evidence="2">
        <location>
            <begin position="93"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="442" mass="50624" checksum="0D1B94BF547C86D0" modified="2011-11-16" version="1">MLHLINFKYSALLASFKSSASSSHPVPDKVFINQVVQVKGNSQNTNFSMAIYLNLRNKNNNVYLNVIDINVGLNKLNECLGKGLTRPKTELHDLVDLINLSEIKSNGITKDEVAEVYQYLKEAKEEQDLNKWESVLIPYLKKKKTLEALRTIIANKEWEHLPSSKVYRFKEFDVDRDHIVDINFVDAVLALIFTTEGAQLSYVNEDFVPEYTLMYLLTNPIFPIKYTTGLLQLFNKSQNFTLLRQAINTCANIPSFDLLTQLVNINTDKLTLNDLVNRLIGEFSRQEITQCFKSILETNKQATQVETEGDEEHQPINLIHIINRMLELKGHNGWFMIEIIIDVAGLFNWTNENINDLNKIITNKIEALTINSYNLTLVNQVVSLTQNKSRASNKKLLQTDQPTNQLDSILTMSNQNNNKKLDETAIEIAQRIPNYSIETLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQY4</accession>
    <name>G3AQY4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribonuclease Z</fullName>
            <ecNumber evidence="4">3.1.26.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_140199</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase Z family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="4"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375991.1">
        <property type="nucleotide sequence ID" value="XM_007375929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQY4"/>
    <dbReference type="FunCoup" id="G3AQY4">
        <property type="interactions" value="1222"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQY4"/>
    <dbReference type="GeneID" id="18870275"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140199"/>
    <dbReference type="eggNOG" id="KOG2121">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006220_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQY4"/>
    <dbReference type="OMA" id="MSHCKHT"/>
    <dbReference type="OrthoDB" id="527344at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990180">
        <property type="term" value="P:mitochondrial tRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07718">
        <property type="entry name" value="RNaseZ_ELAC1_ELAC2-C-term-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047151">
        <property type="entry name" value="RNZ2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027794">
        <property type="entry name" value="tRNase_Z_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553:SF49">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13691">
        <property type="entry name" value="Lactamase_B_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNase Z endonuclease" evidence="11">
        <location>
            <begin position="6"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="856" mass="97685" checksum="1E097721D7A362AD" modified="2011-11-16" version="1">MFSVTTLTHITDDTSRPLVALSTREGNRYFFGKVPEGTQRVLNFIRWRFPKLEGIFMTGTISSWADIGGLPGLFLTISDATKKSITVFGNSKILSYIIATWRWFVFRKGIELRITEAQEKSIYGDKEISVTPIKISNDKPQDNTPDSALFPALRKLASLMFPLDTSKVNSRDPDSYMSDPSETDLHTHVQLPPSTTFFPVQQYSYSYSIRLLPLRGKFDPKKAIALGLKPGPLFRELSDGRSVTNEAGELITPDQVVSPSRPLKKVLIIDIPDQSYYANTVNSNEWFVQDEDRGFEEIGIVYHFLAADIEFDLEKYKSDFLSKFPQETQHVISHPSISNNTLVNKRIGSATLTLKSLMNENFNLPHQDEYVSVLTQDNIVRLHGCQSYNILPSGVEVDNSAIENLSNQQLFEAEVAPLNLGKSYDELANVNFDITKEGDLSLKDRVHICTLGTGSAIPSIQRNVISTLVRIPWQNTDGSITYRGILLDGGENTIGSLWRTFGHSNKAHAIQILRELSVIHLSHLHADHHLGIVSMINEWFKHNDADKKLFLVVPSNYITFLQDWYSLEANYHEHFDINRLECFSCEDFLAERRIPESSKVSLANFEIQFDQGNIHQKIPEEPLSPINFHRINELYDAVGLHSIETVRAIHCYHSYSSIFRFKLDANQTFQLSYSGDTRPNPKFAEVGYDSDLLVHEASLENFLIEEALAKKHSTMIEAVCTSKVMKCPKLILTHFSSRYGNSNNCVPKHELEPLAKSLDEYLITHRAYPNIFSYDSRSYIDYKDLDLCFAFDWMNVRYGELHLQEQHWDKIKELFQSSMLRDPDDEKLEEKRAAKRLERLAAKKASKKQRVNGETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQZ9</accession>
    <name>G3AQZ9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">PQ-loop-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_56465</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the laat-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376438.1">
        <property type="nucleotide sequence ID" value="XM_007376376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQZ9"/>
    <dbReference type="FunCoup" id="G3AQZ9">
        <property type="interactions" value="349"/>
    </dbReference>
    <dbReference type="GeneID" id="18872036"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56465"/>
    <dbReference type="eggNOG" id="KOG2913">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019699_1_0_1"/>
    <dbReference type="InParanoid" id="G3AQZ9"/>
    <dbReference type="OMA" id="DMCIFIQ"/>
    <dbReference type="OrthoDB" id="8048523at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098588">
        <property type="term" value="C:bounding membrane of organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015101">
        <property type="term" value="F:organic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034488">
        <property type="term" value="P:basic amino acid transmembrane export from vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034490">
        <property type="term" value="P:basic amino acid transmembrane import into vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000011">
        <property type="entry name" value="PQ loop repeat protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000012">
        <property type="entry name" value="Vacuolar membrane PQ loop repeat protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF35">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER YPQ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW31660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="336" mass="37842" checksum="9556F0C9C5626681" modified="2011-11-16" version="1">MTPPPPIVIDAQAISGITGSISIACWIIVFAPQIYENFRRKSSEGLSLSFIILWLAGDVFNVLGSVLQGVLPTMIILAVYYTLADIVLLWQCMVYGHGEKTDLIHLSPANPMSEDVDVLETVLSREHHHHHNTEQEEVDYISRDLEASSSTSSTSSSSSKLSQLQTVFLNTLMVALVILAGVIGWYISYVKDSQHKKHHPGHVRRPEDLVYDPLAQVFGWLCAVLYLGSRVPQIVLNYERKSCDGISFMFFLFACLGNLTYVISILSIDTSLHYLWVNSSWLAGSLGTLGLDFTIFIQFFLYNESIDSESENCWCKKRVHMELPRAWSVQTQILKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR13</accession>
    <name>G3AR13_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_62285</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376452.1">
        <property type="nucleotide sequence ID" value="XM_007376390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR13"/>
    <dbReference type="FunCoup" id="G3AR13">
        <property type="interactions" value="838"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR13"/>
    <dbReference type="GeneID" id="18874245"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62285"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025000_4_0_1"/>
    <dbReference type="InParanoid" id="G3AR13"/>
    <dbReference type="OMA" id="RINWASK"/>
    <dbReference type="OrthoDB" id="8093034at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043488">
        <property type="term" value="P:regulation of mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034063">
        <property type="term" value="P:stress granule assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050825">
        <property type="entry name" value="RBM42_RBP45_47-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640:SF5">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="71"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="163"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="363"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="248"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="430"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="269"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="298"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="321"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="457"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW31674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="478" mass="53164" checksum="41AEC8A9F94589C0" modified="2011-11-16" version="1">MTEAEPPHSTTPQVEETANQEQQVQNVDQPQQQQQLDEQQENQTPSKPDTKETTPITPASAVEGGREVSNKILYVGNLSKSISEEFLKDTFASAGAIQSVKILNDKNKPGFNYAFIEYENNQAADMALNTMNGKILQNFEIKINWAFQSATITTANTPEDPSFNIFVGDLSPEINDEKLKQAFSKFKSLKQAHVMWDMQTSRSRGYGFVTFSNQSDAELALQTMNGEWLNGRAIRCNWASHKQLNNQNQGYRNLNSNHNNRHNHHNNHHNNNNNHNTKFRPFNNNNNGNTNGHVFPQQLINPGFNNNNQFAPQVLPGGLQDNGNNNVKNNGQQGATGGNSGNIPAMSPQSYDIVLRQTPSWQTTVYLGNIAHFTQQGDLIPLLTNFGYIVDFKFHPERGCAFVKYDNHERAALAIVQLAGFNINGRPLKCGWGKDRPPPPPPGPMGGHQFQSFPRGVVPPGPPPPGPPHPQMYGQGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR30</accession>
    <name>G3AR30_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62297</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376469.1">
        <property type="nucleotide sequence ID" value="XM_007376407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR30"/>
    <dbReference type="STRING" id="619300.G3AR30"/>
    <dbReference type="GeneID" id="18874256"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62297"/>
    <dbReference type="eggNOG" id="KOG1270">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_849913_0_0_1"/>
    <dbReference type="InParanoid" id="G3AR30"/>
    <dbReference type="OMA" id="LAFHHIH"/>
    <dbReference type="OrthoDB" id="3647at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861:SF1">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="313" mass="35606" checksum="F26E0BC3E4DD8557" modified="2011-11-16" version="1">MTQTEHQKVIAANNANFNEDFVKAYEKRDSQRILAILFVKYLLEYDFEHPTTRKSAAETEQILGDPNLETNGLSVAKDLPDPSTYLTKFPNSIFKPGMKLLDFACGSGIVTELFVPYLKGGERSELYGMDINSIFLEYFNKRASRHASFDLDFKSFQYDVLDQAVQDELDDKFANHFDAIFCTISYHHIHNYELVTKKLATFLRPGGWLFIIDFYNEDVETAKGDTSQAVQHMGGLKIDALNRTLGEIAGLVNVSSAREFRTYNWSPEHFIVNHSRQEILDKLSKGQLPSKVIDGEVNYLIDSSLIYAVGQRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR72</accession>
    <name>G3AR72_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PRE3</name>
        <name evidence="12" type="ORF">SPAPADRAFT_61824</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376025.1">
        <property type="nucleotide sequence ID" value="XM_007375963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR72"/>
    <dbReference type="FunCoup" id="G3AR72">
        <property type="interactions" value="1019"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR72"/>
    <dbReference type="MEROPS" id="T01.010"/>
    <dbReference type="GeneID" id="18874068"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61824"/>
    <dbReference type="eggNOG" id="KOG0174">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_5_2_1"/>
    <dbReference type="InParanoid" id="G3AR72"/>
    <dbReference type="OMA" id="TFIYGYC"/>
    <dbReference type="OrthoDB" id="7854943at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03762">
        <property type="entry name" value="proteasome_beta_type_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000010">
        <property type="entry name" value="Proteasome subunit beta type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6 11" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="217" mass="23753" checksum="A9C63A5CB7EE43BC" modified="2011-11-16" version="1">MNGIKVDINHLKKGEVNLGTSIMAVKFQDGVILGADSRTTTGSYIANRVTDKLTQIHDTIYCCRSGSAADTQAVADMVKYYLQIYAAQLPPNETPTTEIAANVFQEICYNNKDNLTAGIICAGYDKKNGGSVYSIPIGGSIHKQDYAIAGSGSTFIYGWCNENFKQGMQKDECVDFIKTALSEAIKWDGSSGGVIRMVILTEKGVERLIFYPDEYQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARE9</accession>
    <name>G3ARE9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Adherence factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62350</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376534.1">
        <property type="nucleotide sequence ID" value="XM_007376472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARE9"/>
    <dbReference type="STRING" id="619300.G3ARE9"/>
    <dbReference type="GeneID" id="18874285"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62350"/>
    <dbReference type="eggNOG" id="ENOG502S8GZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_546421_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARE9"/>
    <dbReference type="OMA" id="WRFISSE"/>
    <dbReference type="OrthoDB" id="2350934at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="405"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="437"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="470" mass="52832" checksum="5039F70D5391E20E" modified="2011-11-16" version="1">MMSSYDNNNTNSSNFHHPHYPGQQVGVPHTMKDNAYEGQPRYYTSPMVMQQPQQGYLMNNPNAQYGLNQQMMYPPPPPIANYAQQAPQQQPQQAAVAQQQSQQGVNQYFDPSMPNNYLLIQQQNQGNLSMTPNQANATPQQAMAQNFYNPSYSQFHQGAHPHIQPTNISHKSASVGSNQQVPGGANANASSAAAGNAVSATSSGSGNNATANNNAASSVIYPDFPERLQPLLPIPPLSRAPTRPDLNMNLSQKRAKRKSKFTKQQDEMIVSLKKKGKSWVEIAEITNVGSYLAARNRYQVIVGQQGNNNSSSWDLRDKVHLQEILDAGELEKWRFISNELNKSSNKNFTDLECREMIRELFWSNPASFGVNEETINECLKEKKLTDKSIEQRDQQLKKRADIVEGKFVDPKKEPAASANNAGVDKKDHYYPPPLPHPYQRQYQQQQVQQPPPQQQPQQQVNQFSYGKPFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARU5</accession>
    <name>G3ARU5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NADH dehydrogenase (Ubiquinone) complex I, assembly factor 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_61926</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NDUFAF6 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376140.1">
        <property type="nucleotide sequence ID" value="XM_007376078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARU5"/>
    <dbReference type="STRING" id="619300.G3ARU5"/>
    <dbReference type="GeneID" id="18874113"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61926"/>
    <dbReference type="eggNOG" id="KOG4411">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037269_6_3_1"/>
    <dbReference type="InParanoid" id="G3ARU5"/>
    <dbReference type="OMA" id="MINAREQ"/>
    <dbReference type="OrthoDB" id="270318at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21181">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21181:SF13">
        <property type="entry name" value="NADH DEHYDROGENASE (UBIQUINONE) COMPLEX I, ASSEMBLY FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="380" mass="43556" checksum="23CB31055C128F00" modified="2011-11-16" version="1">MLRSQLKRQVRKYSSTYDTLVYNAQQNIIQSLEANDRSSYLLAQYIPDVARNAFLAIRAFNLEINKISDGGQNSSSIASQASREMTGRLGVSTVDLKFKFWSDLVSRVFIEDPYSEKDLGEPIAILLRDALRNDLNLDVDYLLKFLQTRRLFVKGGTFQTVNDICSYGEGTYSQLNYLTQGLLLSPSISPSSIRLLECSTDLQAKISDIAAHIGQATAVGSMILGLQYYATSRNQVTMPIDLMTKFDLSQESLLRLCQGHIKTNMEQEEIRDKLKNVVYETAITANDHMLTARDKLSKTEQEIKEILSSHPHDQLLSRHGKKWRRNIPDVLFTPFMVAIPTTLYLNRLEKYDFDVFSPKLQQKEWRLAWTSFKNYHQRIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARX9</accession>
    <name>G3ARX9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_62444</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376606.1">
        <property type="nucleotide sequence ID" value="XM_007376544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARX9"/>
    <dbReference type="FunCoup" id="G3ARX9">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARX9"/>
    <dbReference type="GeneID" id="18874318"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62444"/>
    <dbReference type="eggNOG" id="ENOG502TDNM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_963100_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARX9"/>
    <dbReference type="OMA" id="LGPYNHE"/>
    <dbReference type="OrthoDB" id="4033880at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="2">
        <location>
            <begin position="14"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="306" mass="35251" checksum="ABDC9D1F09DD3ACF" modified="2011-11-16" version="1">MTHIIQSIKNITSSTTDSKIKDATNIEPLGPYNHELIELAQLTFHKKDLSVIISGVTKRLNPLVKIANEKSGQPQPSTIESQSMRRSTSLTLPSFHLKHDSVPSRSNTFALFSRKDKCHYDLDEKTSLSLLKTCAVVLYLLQNGSHNYVDWMQSNYHTYFIPLRKLSYPPKYYETIRYKLRKIINFMEYPEQLQDNRNHIHKLRTDIMTPGVKRTSLDIDLLHVQQQQSPLRERRYSLDREYTLASPPPGPFGFTSLPGTVNEVSMAPRSSLHEPGKRRSSHSRMQPEHPSSPPRTPNPRRLSSMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS38</accession>
    <name>G3AS38_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carbohydrate kinase PfkB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_72581</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376665.1">
        <property type="nucleotide sequence ID" value="XM_007376603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS38"/>
    <dbReference type="STRING" id="619300.G3AS38"/>
    <dbReference type="GeneID" id="18875444"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72581"/>
    <dbReference type="eggNOG" id="KOG3009">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056966_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS38"/>
    <dbReference type="OMA" id="ACADMSV"/>
    <dbReference type="OrthoDB" id="198885at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004730">
        <property type="term" value="F:pseudouridylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42909:SF1">
        <property type="entry name" value="CARBOHYDRATE KINASE PFKB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42909">
        <property type="entry name" value="ZGC:136858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="2">
        <location>
            <begin position="11"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="371" mass="41135" checksum="D14FD7210C251279" modified="2011-11-16" version="1">MNPGSKLQDSNPGTTTNSVGGVGFNISLASKYGYANSKLISQIGNDFAGTFILDKLKHEGIDTSGITLANGNTAQYTCIHDSAGELVVACADMSLIEQDFSENIIKEIEYNQPNLIAMDCNLSPTTTCKVLSSSLSNIILEPTSHIKAKRIAEFNLQCFPNNKIQLITPTIQELNSIYESFQSKGYFNDLDNWFPVLDSFNINSNFRDKLDRINTPTIKDLLAQGVFQQCFHLLPYFQNILVKLGDKGVILISLSTTIDDYKSIPTTSQYRPEILLTSQGTKLSQGRMGISVEYFPIPQENRNLDIVNVTGAGDSFFGYLAAYLSRFNWLNSEITSVEQVWNKWECIYKCQLASGLSLVSTNAVSEEISKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASG6</accession>
    <name>G3ASG6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_56990</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377117.1">
        <property type="nucleotide sequence ID" value="XM_007377055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASG6"/>
    <dbReference type="STRING" id="619300.G3ASG6"/>
    <dbReference type="GeneID" id="18872088"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56990"/>
    <dbReference type="eggNOG" id="ENOG502S8MB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132731_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASG6"/>
    <dbReference type="OMA" id="MREIPRY"/>
    <dbReference type="OrthoDB" id="5397701at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37331">
        <property type="entry name" value="YALI0F11671P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37331:SF1">
        <property type="entry name" value="YALI0F11671P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="181" mass="20500" checksum="B9E5A01B883DD06D" modified="2011-11-16" version="1">MIRSIQLSTKRQLQLQLRTLATYASNPQIYIHKVNPLNYKFSLSKSPDAIEIGSSPTADPEPSTFTDNPKFKELLNSTIYSKIQDDFTFVMEAGVNANSFMPIYDLRETPNYARVPATENIFGYVRVDSKGKIVPESFQANGMYRLINGRCGLCKFSDFLHEQLQTQSEMQVVKQNVNAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASM6</accession>
    <name>G3ASM6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_155857</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="2"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376744.1">
        <property type="nucleotide sequence ID" value="XM_007376682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASM6"/>
    <dbReference type="FunCoup" id="G3ASM6">
        <property type="interactions" value="416"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASM6"/>
    <dbReference type="GeneID" id="18871033"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_155857"/>
    <dbReference type="eggNOG" id="KOG0942">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002173_2_3_1"/>
    <dbReference type="InParanoid" id="G3ASM6"/>
    <dbReference type="OMA" id="DCFELQM"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000011">
        <property type="entry name" value="Putative Ubiquitin-protein ligase E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044611">
        <property type="entry name" value="E3A/B/C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700:SF2">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="6">
        <location>
            <begin position="588"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="5">
        <location>
            <position position="903"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="935" mass="108606" checksum="69967F02765FDAE8" modified="2011-11-16" version="1">MINFSGSSTKRRVVNLGDRKTPVSSRSFLEQSRITRIQRDQQRTQEKSAVTLQCYIRRYLDLMRYSQSLINESKQEVSNEQEWIRSIKINIILCKWYIPSHKDVGLIQTIYSKLDQSGWALHENLYNSLLQALIGVFILIFQTIKLLMDKFNSDYNMKVANLTSYLYKFLHQSQIQGDQKQEVLDMIYAINIRDSFENFIQVLKLTYPGPNINYLNILRPIILQNQEELGQFEELDLIQMLASYLAILGSVDYTKDDFVIIAALLVNTNCIVGSEVETDDNDSQVDQGRIVHVDKSVVTQLEKLYTSNFIKSTITLLTGENNSLALKILLPLITLIPSLTGKISMFISIIPDCFYWFYQTIQGNEIFDMFLNSDKDYIPSEQLETHSNDKFWKLVLLFEKSYSYWLIVSNDLESFADDKLSLIHVKQYLKFLRTLCLTLVFNNNFPHYSELKQISIGLLNQLYLKNLRLKFLNQNFWIPRSITFDIDVLIRVVAEEEVQDSDEEEPKFSVTRTVPNEVKARVEILKTMPFFISFKDRVRIFQALIELDRMRASNTSFNFFSFDEPARLSADIRRENLLVDAFNAFNKSGASFKNRIQVVFYNEYGQEAGIDGGGITKEFLTSVVKEGFNPENELKLFKETLSHNQLYPNNDIYDKLHLGLEMEAQQVRLQYLRFLGMVVGKCFYENVLIDISFAPFFLNKWCNMKNSINDLNYLDSELFVNLMKLTKMTNEELEALDLNFTIYEKVNQKTYLFDLAPNGQSTQVNSSNILQYIHQLSNFKLNQSLTIQTRSFLQGLFSIISSNWLNMFDCFELQMLISGEPTDVNINDWKLNVEYGGYLENDLTIKYFWQVVEEMTKEQRFKLIKFVTSVGRVPLLGFGSLNPKFGLRNSGRDGDRLPTASTCVNLLKLPDYQNKQILKEKLLYAISIDSGFDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASM8</accession>
    <name>G3ASM8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_142233</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376747.1">
        <property type="nucleotide sequence ID" value="XM_007376685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASM8"/>
    <dbReference type="GeneID" id="18870433"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142233"/>
    <dbReference type="eggNOG" id="ENOG502QTW2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021105_1_2_1"/>
    <dbReference type="InParanoid" id="G3ASM8"/>
    <dbReference type="OMA" id="HYYLLIG"/>
    <dbReference type="OrthoDB" id="511599at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056808">
        <property type="entry name" value="HTH_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36168">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36168:SF1">
        <property type="entry name" value="ORC1-LIKE AAA ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24913">
        <property type="entry name" value="WHD_AAA_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase" evidence="1">
        <location>
            <begin position="115"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="AAA protein C-terminal winged helix" evidence="2">
        <location>
            <begin position="341"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="541" mass="62844" checksum="8DD230FA9B52051A" modified="2011-11-16" version="1">MEPIVENFLQELQEEEPKDESKKDKFKSFIFKCLETMAITISSVGVLALAGFCYHKFYDIHVLSKINTAFEAGDPASQLKMHRSDHKDSTYWVERSQQKLLDDIISGNVIGRYFLIIGEKGTGKTSLILESMKKIDGYNVAMFDAHSDPEIFRIRLGKAINFTFNEDYIGSLFSIKGQRDTTALLDIERAFNKLEQLASARVKKVGKPLIMIINNAHLIKENEEGIKLIELLQQKAEALSGSGLVTMIFNSDDYWVYENLKQLGTRLELINVRDLDRKETVSALKFIRQRYFPEQIPEDVCNTVYDLIGGRPQHINHVARNKDIIQACHEIIDREKTWFLNKCGLLGNDMDDDVMESGKFSSSAMLLMKELVEMDKKQPKSVDHHLPELPLWRCRQIMTRNDYIQHYDNLNIFTIDSHSRVRADSMPMMRAFHEIASQPNFDELLQDTIDRVADIESLERTREIVLKDLSLGGNYEVKKDKDKYAMKLNKSRKQIDVHGDWTEYNNDMNEDDDEDLFLEGISRKEARRLWKQRMEKFKETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASQ4</accession>
    <name>G3ASQ4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_52290</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cclA family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377151.1">
        <property type="nucleotide sequence ID" value="XM_007377089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASQ4"/>
    <dbReference type="FunCoup" id="G3ASQ4">
        <property type="interactions" value="104"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASQ4"/>
    <dbReference type="GeneID" id="18871655"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52290"/>
    <dbReference type="eggNOG" id="KOG2626">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014420_4_0_1"/>
    <dbReference type="InParanoid" id="G3ASQ4"/>
    <dbReference type="OMA" id="GFRYTYA"/>
    <dbReference type="OrthoDB" id="10266026at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12872">
        <property type="entry name" value="SPRY_Ash2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037353">
        <property type="entry name" value="ASH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10598">
        <property type="entry name" value="SET1/ASH2 HISTONE METHYLTRANSFERASE COMPLEX SUBUNIT ASH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10598:SF0">
        <property type="entry name" value="SET1_ASH2 HISTONE METHYLTRANSFERASE COMPLEX SUBUNIT ASH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="5">
        <location>
            <begin position="123"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW31118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="484" mass="55563" checksum="8242028FA7028B5E" modified="2011-11-16" version="1">MSELTKEVSEEPSIEPQVQELTAEIDSSQPIDSSTSANAHSYSTRSKHRAQQDEHELKSKLKQKEHNVVVYPRLKPIPFKHSDLLVNSVQPSFTEATTVRDMKFYQCEDLPINKRGFKYKPCRPNPEFQSNLYSTTDIPPYHVCVDLFDRSPGIIFDNDLTTVSTLQGWRSARANVGIREGSYYFEFEIVNSCEDNSHVRIGLARREASLEAPVGFDAYSYGIRDISGEYITTSRRKTVCIDQGFKSGDVIGFLVELPSLSKHKQAVAKFVEEKLIEAKVNPNKETGKKKRKTTKKPVDLQENVKFNIHDNIIRDQIPIRYKNSLYYEQFEYTTTKTMDHLLNPITVFGEKAVLENDDKTKSIPIIPNSRIRLFKNGIEQQEIKDLYSFLPTNLEHTDDLSLSANLNQQQNPNYRNSDDGTLGYYPMLSTFQHGVVKLNPGPDFKYPPVTSVKPLCSRYDETVVEEWLWDIIDEVEAEYLDSFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASY9</accession>
    <name>G3ASY9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_68010</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376803.1">
        <property type="nucleotide sequence ID" value="XM_007376741.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASY9"/>
    <dbReference type="GeneID" id="18875194"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68010"/>
    <dbReference type="HOGENOM" id="CLU_650809_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASY9"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="422" mass="49680" checksum="8A996A2BEEC96948" modified="2011-11-16" version="1">MKWFWKKKYNGRLPLLPMNFSVEIFYEIFSHLNQFDILQVILINKQFYELGIRELYKCLLVDNGTDRYIISQHMRTSFTTKYTIIDGIQLFKKKVFKRKRMKFVQLVIFRRDTCLDAIYQDMIRKYPWVEIRIMDPDFMSDKGPFYTNQKLKLQAKTLTRLKIEPNLFIKVEDDNYSIIEYVINTTPTPVLLSSIPKLKRLETLCILDPQSNHLITDLQSSQLSDICLKTLILKVSSIQLSSLGEIFNLTTITKLKLEFTKNVAAPYEELKTLTSNLPRLNSISINWRFTNAVIIMDYLVGKNIRHISVKTNEEIDYSYLKYYPTNFMKHQQLISLHLWGNPPTPPPPVKTRSRLISWFSKKETPCDDLHTSKINAIRGFIDGGFPNLNQIILNGWFFMITGKREVKLEHWPNSHMSCICCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATF4</accession>
    <name>G3ATF4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycine cleavage system P protein</fullName>
            <ecNumber evidence="7">1.4.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_142446</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The glycine cleavage system catalyzes the degradation of glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 7">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GcvP family.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="7"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376950.1">
        <property type="nucleotide sequence ID" value="XM_007376888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATF4"/>
    <dbReference type="FunCoup" id="G3ATF4">
        <property type="interactions" value="714"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATF4"/>
    <dbReference type="GeneID" id="18870446"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142446"/>
    <dbReference type="eggNOG" id="KOG2040">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004620_3_2_1"/>
    <dbReference type="InParanoid" id="G3ATF4"/>
    <dbReference type="OMA" id="RNLICTC"/>
    <dbReference type="OrthoDB" id="6537869at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016594">
        <property type="term" value="F:glycine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00613">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000005">
        <property type="entry name" value="Glycine dehydrogenase (decarboxylating), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000007">
        <property type="entry name" value="glycine dehydrogenase (Decarboxylating), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003437">
        <property type="entry name" value="GcvP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049316">
        <property type="entry name" value="GDC-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049315">
        <property type="entry name" value="GDC-P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00461">
        <property type="entry name" value="gcvP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773:SF1">
        <property type="entry name" value="GLYCINE DEHYDROGENASE (DECARBOXYLATING), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773">
        <property type="entry name" value="GLYCINE DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21478">
        <property type="entry name" value="GcvP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 6 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="8">
        <location>
            <begin position="68"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="8">
        <location>
            <begin position="533"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="839"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR603437-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1021" mass="111358" checksum="0875BFAB73ADF920" modified="2011-11-16" version="1">MISATTKSLLRSAARVRVSTVASTALKCSTTSFIRHLATANTSSTNYGHVYNPNAQKVSLANLDTFARRHIGPTPANVQEMLKTLGYSDLDEFLTSAIPNHVLIKRKLSVSPAQGYTESEMIEHLQKLAAKNKIVKSFIGKGYAGTKLPAVIQRNLLESPEWYTSYTPYQPEISQGRLESLLNFQTMVSSLTGLPMANASLLDEGTAAAEAMSLSFHNMKGKKKVYVIDTNLHPQTVNVLKSRAGNIGVEIVELPLSTEEGITELNKIIKTVCGGLVQYPGTDGSIHNFTKVGEILHSHKVLFAMACDILALTVLKPPSEFGADIALGTTQRFGVPFGYGGPHAAYFSTTMKYSRKIPGRIVGVSKDRLGQPALRLALQTREQHIKREKATSNICTAQALLANISANYAVYHGPAGLKNIASRVYGFTTLLAKYIGQTEHQVLNDKWFDTLTVKLSGVSADDILAQALSKYNINLFKVDESTVSLSLDETVETADLVNLIELFTGKQVTLEADTELPTIPEELIRTDEILTHEVFNTHHSETAMLRYLHLLQSKDLSLANSMIPLGSCTMKLNATVEMQTLSMPGFTQIHPFAPIDQAQGYKELISEFEKDLNDITGFDATTNMPNSGAQGEYTGLSLIREYHKSRGEYEARNICLIPVSAHGTNPASAAMCGLKVVPVKCLDDGSIDLVDLQEKAELHAANLCSIMITYPSTYGLFEPGIKKAIDLVHANGGLVYLDGANMNAQVGLTSPGDLGADVCHLNIHKTFALSHGGGGPGQAPVCVKEHLKPFLPGHTFNADVTKPDSIQAVNSAPYGSASVIPVSYSYIKMLGANALPYVSAVAMLNANYMVKKLQPYYSILFVGKSTPHCAHEFILDLREFKAQGIEAIDIAKRLQDYGFHAPTMSFPVAGTLMIEPTESENLAELDRFVESLIKIRSEIDAYINGDKSGMVLKNAPHSMQDVVATPQEEWEARGYTREQAAYPLPWLKNNKCWPTVSRLDDTYGDMNLICTCPTVEEIAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATL2</accession>
    <name>G3ATL2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_62877</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Complex I functions in the transfer of electrons from NADH to the respiratory chain. Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion inner membrane</location>
            <topology evidence="1 11">Single-pass membrane protein</topology>
            <orientation evidence="1 11">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the complex I NDUFA13 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377008.1">
        <property type="nucleotide sequence ID" value="XM_007376946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATL2"/>
    <dbReference type="STRING" id="619300.G3ATL2"/>
    <dbReference type="GeneID" id="18874505"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62877"/>
    <dbReference type="eggNOG" id="KOG3300">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119720_1_0_1"/>
    <dbReference type="InParanoid" id="G3ATL2"/>
    <dbReference type="OMA" id="YGIREQH"/>
    <dbReference type="OrthoDB" id="3308at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009346">
        <property type="entry name" value="GRIM-19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12966:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12966">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE 1 ALPHA SUBCOMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06212">
        <property type="entry name" value="GRIM-19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW30975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="128" mass="15247" checksum="0827B6042052D5DF" modified="2011-11-16" version="1">MQDLPPVGGYEPIQWKRNIPARGFSGNVYFWGITAFMAFGFYRYYKGVDEQRELLREKAYARFNLEPLLLAEEDRNLARRYYAELARQKLVRESMSPETRAKFDEEVYHDKSKFRFPRFTSGIDAKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATS1</accession>
    <name>G3ATS1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sodium/hydrogen exchanger</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_63144</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377268.1">
        <property type="nucleotide sequence ID" value="XM_007377206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATS1"/>
    <dbReference type="FunCoup" id="G3ATS1">
        <property type="interactions" value="362"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATS1"/>
    <dbReference type="GeneID" id="18874615"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63144"/>
    <dbReference type="eggNOG" id="KOG1965">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005912_5_1_1"/>
    <dbReference type="InParanoid" id="G3ATS1"/>
    <dbReference type="OMA" id="FTYVRRF"/>
    <dbReference type="OrthoDB" id="196264at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
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        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
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        <property type="entry name" value="NaH_exchanger"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00840">
        <property type="entry name" value="b_cpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF187">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01084">
        <property type="entry name" value="NAHEXCHNGR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0050">Antiport</keyword>
    <keyword evidence="6 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0915">Sodium</keyword>
    <keyword evidence="8 9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="172"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="242"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="361"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="440"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="12">
        <location>
            <begin position="63"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="497"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW30297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="612" mass="68252" checksum="9E53241F0564A220" modified="2011-11-16" version="1">MTSLTLYAAKYLVKRALDLDDDTPDLTTPDEDLEDSNPVENEIFSSWALFILLFLLSSALWSSYFLQQRKIKAVHETVLSIFCGMFVGLIIRMSPGHYIQDAVKFNPGYFFNILLPPIILNSGYELHQANFFRNIGSILTFAIPGTFISALVVGIILYIWTKLGLDGIKLEFVDALAVGATLSATDPVTILSIFNAYKVDPKLYTIIFGESLLNDAISIVMFETCQKFHGHPVHFSSFFEGIGLFLMTFTISTIIGFLIGIFVALILKHSHIRRYPQIETCLILLFAYESYFFSNGAHMSGIVSLLFCGITLKHYAYFNMSRRTQIATKYVFQLLAQLSENFIFIYLGLSLFTEVELVFKPLLIIITFASICVARYSAVFPLSNFLNFLFRTRFEKFSGMNANSIPDEISHSYQMMIFWAGLRGAVGVALAMGFQGEAKWTLLATVLVVVVLTVILFGGTTASMLEILGIKVGCVDETADSDDEFEIEAPRIALSNSTNTPVMGHRKNQSQSTHYKDVVQHRNHSSSSVSSHPDLLDAFDDGELLSNDIDDLITAPTPSLPNHNRSRSDGGAGVLGAFLSAEEHAKWFTRFDEEVLKPVLLDTLPKNRDGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU78</accession>
    <name>G3AU78_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GP-PDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_63287</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycerophosphoryl diester phosphodiesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377425.1">
        <property type="nucleotide sequence ID" value="XM_007377363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU78"/>
    <dbReference type="FunCoup" id="G3AU78">
        <property type="interactions" value="107"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU78"/>
    <dbReference type="GeneID" id="18874681"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63287"/>
    <dbReference type="eggNOG" id="KOG2258">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_729591_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU78"/>
    <dbReference type="OMA" id="RIQFGIW"/>
    <dbReference type="OrthoDB" id="1058301at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034479">
        <property type="term" value="F:phosphatidylglycerol phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08570">
        <property type="entry name" value="GDPD_YPL206cp_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052271">
        <property type="entry name" value="GDPD-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42758">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL PHOSPHOLIPASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42758:SF2">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL PHOSPHOLIPASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="GP-PDE" evidence="8">
        <location>
            <begin position="26"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW30454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="390" mass="46042" checksum="4B276057E7FA87F9" modified="2011-11-16" version="1" fragment="single">MFSRFRTTFTTPYKYLTLYRRMSVSPVIVGHRGFKGNYPENTMIGFNKCFETGATIIETDLWLAKDNVIIISHDPSTKRVFVDSEGNETDYKIIDTEYDPILKQLKTIEGGYPLMTFREVLQWFKAYVVDHEGEFKLQLDIKRFNPPKILKQIIEDLLSVEPNMGFWYNRIQFGIWDLNFLKYLNQDDYFQDKFAEQQFDIFNITVSWRDAIFYVSYNDYLDALGGDRVKIKLTGVSLIYISTWSQGFLTKFVPLLKVHGLKLFSWTINKKFQYEYLCKVGELGGLVEYGVISDHPDTMVSYADHKEELEEVGETTRLTPTTESLRSSLTFKQWFSYFIFQVFHKMGSDKKLTDDEKQYDSIVDENKVRVIKVNEWVIWMFQTMQKFGIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU86</accession>
    <name>G3AU86_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_157531</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377433.1">
        <property type="nucleotide sequence ID" value="XM_007377371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU86"/>
    <dbReference type="GeneID" id="18871086"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157531"/>
    <dbReference type="eggNOG" id="KOG1339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013253_9_1_1"/>
    <dbReference type="InParanoid" id="G3AU86"/>
    <dbReference type="OMA" id="YWANFTI"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442577" description="Peptidase A1 domain-containing protein" evidence="4">
        <location>
            <begin position="17"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="5">
        <location>
            <begin position="53"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="442" mass="49465" checksum="6402BE4ABAA08AA4" modified="2011-11-16" version="1">MLHLFYIFQYVLAVGAMSTGGVHRLDFEVVTRDSSAQPEPLMKQNMLNDKNMYLARFFIGSNRDEVKLQVDTSCADFWVMGSGVDCLPHIPRRAQYDIWTRCSCEDIAAPTQMVESSCISQGSFRFNDSTTFERHDKEYYQENHLTRGKTAQGFWGLDDVSFANKSIRIPFGVVNETNLDEGILGLGFPEENGNLSFPYELKKNGLIDRAAYSLYFNSTNASFGSLLFGGIDHAKYSGNLKSIAAVHNQTISYIAVDVNRILMTMNNTTTVVIGNKKTTFMLDTSSSLSRFPMAVLNTMADALGGRVYKETNDLIVQRCPDTLEFNIDFDGQLISIPGEAFMYKSIGDHCLIRIQVDDLTPAIGQDLLRSMYVVVDLEQEEVSVAEAKFTQDYEIELLPPVGKNRSYSVEFTGTEREESASHSNSVEILIAGLMWLALLVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUL9</accession>
    <name>G3AUL9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF3074 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_143874</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377547.1">
        <property type="nucleotide sequence ID" value="XM_007377485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUL9"/>
    <dbReference type="STRING" id="619300.G3AUL9"/>
    <dbReference type="GeneID" id="18870579"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143874"/>
    <dbReference type="eggNOG" id="ENOG502QTT5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078586_1_0_1"/>
    <dbReference type="InParanoid" id="G3AUL9"/>
    <dbReference type="OMA" id="KWIMATT"/>
    <dbReference type="OrthoDB" id="240546at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024500">
        <property type="entry name" value="DUF3074"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40370:SF1">
        <property type="entry name" value="DUF3074 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40370">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11274">
        <property type="entry name" value="DUF3074"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3074" evidence="1">
        <location>
            <begin position="52"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="215" mass="24085" checksum="714320CD6E5DACAD" modified="2011-11-16" version="1">MSEQRDTLVKESIAKIDSINKWHPSKTFNYSLESGGTVQVKTFKTTMNNQTWFARTSTVPKAYHDSLAKYIAGDSEYGKTHSDYEKEYIHEITSVQCTSLKQYSDGWNYQVKAEYDFGAFIKKRVFYELVDIYKGDGFIYVISSAAKPGSDETSSVVGTYDSIEKVSWEGDNLMWTMATTSNPGGLIPGWLTNLSMPGAISKDVPSVLNWIGGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUQ9</accession>
    <name>G3AUQ9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63444</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377586.1">
        <property type="nucleotide sequence ID" value="XM_007377524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUQ9"/>
    <dbReference type="GeneID" id="18874754"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63444"/>
    <dbReference type="HOGENOM" id="CLU_2591316_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUQ9"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="80" mass="9522" checksum="1106D609FCD133C5" modified="2011-11-16" version="1">MDEIHSHISEISDQDGSLHHHNSVLHDDKDRLNQEISEMRKEYDELLLYFVSYRAKFQEQERRSFKKLKLNDSGADEDCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVA8</accession>
    <name>G3AVA8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_73362</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UFD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377677.1">
        <property type="nucleotide sequence ID" value="XM_007377615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVA8"/>
    <dbReference type="STRING" id="619300.G3AVA8"/>
    <dbReference type="GeneID" id="18875480"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_73362"/>
    <dbReference type="eggNOG" id="KOG1816">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012913_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVA8"/>
    <dbReference type="OMA" id="LIFPTRH"/>
    <dbReference type="OrthoDB" id="193703at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.50">
        <property type="entry name" value="Ubiquitin fusion degradation protein UFD1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.130.10">
        <property type="entry name" value="Ubiquitin-protein ligase E3A, N-terminal zinc-binding domain (AZUL)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032353">
        <property type="entry name" value="AZUL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042556">
        <property type="entry name" value="AZUL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049439">
        <property type="entry name" value="TRAFD1-XIAF1_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004854">
        <property type="entry name" value="Ufd1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042299">
        <property type="entry name" value="Ufd1-like_Nn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055417">
        <property type="entry name" value="UFD1_N1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055418">
        <property type="entry name" value="UFD1_N2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555:SF15">
        <property type="entry name" value="FUSION DEGRADATION PROTEIN (UFD1), PUTATIVE (AFU_ORTHOLOGUE AFUA_4G04640)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555">
        <property type="entry name" value="UBIQUITIN FUSION DEGRADATON PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16558">
        <property type="entry name" value="AZUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21366">
        <property type="entry name" value="TRAFD1-XIAF1_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03152">
        <property type="entry name" value="UFD1_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24842">
        <property type="entry name" value="UFD1_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23580">
        <property type="entry name" value="Znf_XAF1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin fusion degradation protein UFD1 N-terminal subdomain 1" evidence="3">
        <location>
            <begin position="9"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin fusion degradation protein UFD1 N-terminal subdomain 2" evidence="6">
        <location>
            <begin position="101"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="TRAFD1/XAF1 zinc finger" evidence="5">
        <location>
            <begin position="488"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-protein ligase E3A N-terminal zinc-binding" evidence="4">
        <location>
            <begin position="587"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF21366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW29911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="711" mass="82264" checksum="3FA8A86D9DA878E3" modified="2011-11-16" version="1">MSFQFYLSVDQSNDRSLNPYSDKAILPPSVLSQIVEVIPESNLPHPLIFRITNTNAPSGKPVYIGVKEFTAVEDAILPDVIYRKLGSPEQVEITLVQNIPKATSIKLRPDQFYANITNWKFFLENKLNLYYTTLTSGDIMIIQDENLRYELHIDEINGGANTTACIVDTDITLEMVPLNDQLAKEQMLAFNSNPHNNIVEIEHELDVDNLKSFSDSQFIPTIYKIDLTRSKSGIVLKLINRDYDGKKYDSLFNADLIVGLDKIVTLENFHYSTMDQDFDIEYKLDHGKHSDGIKSVEIDYKDDIIVNKLHRIQEELKLDEDDIDKYLYVIPYTWDTNANITLVVEEKGETYAPEEEDIPDENQKQCSNCLKYISVQSYDLHQSFCLRNNVRCVKCDSVFLKKIPETHWHCDLCTNGFYANTPLLQSKHVKLYHLDPYTCSQCPDHTPFNDFFDLVTKHKSTNCPAKMHQCRFCHLIVPQEEATYQDRFENLTHHENSCGNKTNECYKCGKIVRIKDFKKHVKMHELDKVEFNQMNKFTFNKCCNENCINLLSGVSSNEMGLCDICYGPLYVAQHDPTYLKLQIRIERKYMIQLSKGCGNDYCNNEYCRTGNRSLQSKPFKELMQLLNQELFSNIHSPKLPVNKSRKESDVGANKVWFCVNESVSNKKVLLDILRSEGVYEDEIVYKAVNERSDEQAIRSWLREHAITRSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVE3</accession>
    <name>G3AVE3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_63769</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377928.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVE3"/>
    <dbReference type="GeneID" id="18874907"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63769"/>
    <dbReference type="eggNOG" id="ENOG502SQC8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056390_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVE3"/>
    <dbReference type="OMA" id="DHHIGTK"/>
    <dbReference type="OrthoDB" id="4075408at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="414" mass="48484" checksum="B353A42D1156D507" modified="2011-11-16" version="1">MTQLVSTSDALVTLADHILDSIDDLHQVQYKKKGRTYRFVNHTFQRVRQQDKHLIIDPDNLDEDIGLLSAFSILYNINNGEILTQFPDFCCTILSMARQLERNKWFEDENSCVVNIRYSSYDPRDLKDLAEEYIEMHPITENHIKYGVNLMYAAKLNFLHTDHHIGTKLEGLYMRQFIEEYYGEQALNSSDVLIALKSCVHWGNIKGMLYKLGVADIDMTPELIESFDSFPDADEKLRLNVYQRYPSGTSKYSLIRKSLDILSEWRYSRLVPLPHDLDLSWIYQLCHDIETNPIRYHLRSHTKRLCIDPVNLGDLNTKYSAKIKQLLNIVSIIINVFQETGAEFLLQNSKFNNFGPELINSQKQYYEKLLKLKDHIEVYEDKQWNSDDIVIRLDSGDSNNSLYHRITEARGNYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEA4</accession>
    <name>G3AEA4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58847</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373050.1">
        <property type="nucleotide sequence ID" value="XM_007372988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEA4"/>
    <dbReference type="GeneID" id="18872643"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58847"/>
    <dbReference type="eggNOG" id="ENOG502RQG4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016726_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEA4"/>
    <dbReference type="OMA" id="RHNAYFI"/>
    <dbReference type="OrthoDB" id="4034597at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="694" mass="79929" checksum="EDC227CC5C432A9D" modified="2011-11-16" version="1">MILEETAFESLNGIYINPKDQSKRDKISQDTVYTFMLELVNMNYILDKPVIDELCKLSADKGGKFLKQQLLMVKKHLGAHVKHEPFYPNFPQQVMDASHLELFIYAITHYFSCGNWKPVFIKEKRVEINEKNPVVNLHLVSLDELKEYFVKLVNSKTGIPVKHDAFIVDGVRRNWIKTYQSTYSEIPFRETSCKLAVECLKQGKDIGYFVKTTTDVLRIMAVYSNQKPDLTKVVKFTSMKRKERRVLIQLLEKVINIEDVKRHTTIWIRAFHCLHVGEYGGKVNEIATRFRSEKNVPTKNTPLCKAIENKDTDSAIRILKNLPSMFARCLDKLLRDSPDDKKILSEFSMIAVGVESKVLLQALGHFKGSNKIEKRSVFAKSNLMMFERKENQSELTTETANMVIDTLRQALVEKYKALNHFNENSKVYITKEAEGILLPMQIYTGSDSNKRAIARGSRLMTDECDMDKNVIRFFVHWIGKIVDLGAVFIKEDMETTKFINYTHLKEEFAVHSGDIINAPAPKGASEFIDIYINQALKSGYRYVEMNVRSYSGPKFSQLQQCFAGYMMRKEQQAGEIFEPSTVKVKMDLTCEATDINPFMFDLKTREMIWLDIPSHPKVFWLNDLNRNINQTKEILKACLDLPLIKVNMKELLELHVEAGSATIVENREDSNFVVGLGEGDLDLYDFATINSKWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AED0</accession>
    <name>G3AED0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_48686</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373130.1">
        <property type="nucleotide sequence ID" value="XM_007373068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AED0"/>
    <dbReference type="FunCoup" id="G3AED0">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AED0"/>
    <dbReference type="GeneID" id="18871254"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48686"/>
    <dbReference type="eggNOG" id="ENOG502QQ2V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059017_0_0_1"/>
    <dbReference type="InParanoid" id="G3AED0"/>
    <dbReference type="OMA" id="NIMFATR"/>
    <dbReference type="OrthoDB" id="5549748at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07600">
        <property type="entry name" value="BAR_Gvp36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018859">
        <property type="entry name" value="BAR_dom-cont"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10455">
        <property type="entry name" value="BAR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="324" mass="36520" checksum="536FA5AF62B27CF8" modified="2011-11-16" version="1">MSFNFSNFTKDLKSLGDKISTEFNKEVVPLAQRTTRMVQEKIGNVNADEISQLPSEYIEIARKCNSIEQLYKNVLKVTSTYDNEPYDYPSNLQESFTEFGKDITSRVSSLSKATSPAEAQAALIHPKQDLTRPPKTLYHAISRATDPAVLSGADNQHQDINDPLVKGLTLYSTNLNKIANARLGQDQLIRTKFNKPLTTTLRSLIGQSNQIQKKVEQKRIDYDLARKALTNCTNPNKESQLRVDMENAEDEFANTVEDAINIMQNVLENAKPLHEFLELIKAQLAYHKLANELLGSMVEDFEQIIEDSSKQTGTESRESGDFDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEI1</accession>
    <name>G3AEI1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_57809</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372155.1">
        <property type="nucleotide sequence ID" value="XM_007372093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEI1"/>
    <dbReference type="STRING" id="619300.G3AEI1"/>
    <dbReference type="GeneID" id="18872204"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57809"/>
    <dbReference type="eggNOG" id="ENOG502RQ9B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1415988_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEI1"/>
    <dbReference type="OrthoDB" id="4018477at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="117"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="192" mass="20795" checksum="6C5987E9941ACC35" modified="2011-11-16" version="1">MPSTTSPKRQASITTGASFILPFAAAAAAAESGARQNNASTLPSILLNPPSNISQSFNSNHATNLPSINKILRTPIKRANSTRQLLKQQPNPPPHPARASRLPTVTDDTTIDEDNDMTIINNTTTYNNSTVVEHNDTTIPEKNEDDDDEDDDNDFDSRKKDILSSSPLSNPFGTPNSFSVARSLLQLGGHRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEN8</accession>
    <name>G3AEN8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_131769</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373076.1">
        <property type="nucleotide sequence ID" value="XM_007373014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEN8"/>
    <dbReference type="GeneID" id="18869607"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131769"/>
    <dbReference type="eggNOG" id="ENOG502RPWZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_634579_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEN8"/>
    <dbReference type="OMA" id="DWYHNSP"/>
    <dbReference type="OrthoDB" id="4021409at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="375" mass="43333" checksum="0902DB73AF0B153C" modified="2011-11-16" version="1">MVLLGEIVQSQQYFSRLTTGIPSLDQIFITGTGNNQSLTNKIYDFQTAPSCQAMYLVTSSLIMSHLSNEKSVIIIDTLNRFPMKFIIKHPSFKKEWLNDKLMLYDRDTFSKLYAFFIYKKDIPKNCMIIINDFHDLVELYKLEMSSVYEELLLKHHIDMNDTYLHNKKTADNLLIPELPFNSDLIKVSPIAKFESHVKILFSKLIQICITQMSPIFLLGHLDTKFQPYRMKPTSQANSSQSSLTEKGRVVLTPHKFEKISTRIVFYNDWYHKTPHFDETVKSNFINENKLRMVSAARLELPKQKLMHHPVYFAVDDKFYHDKHFALDNKSYSLIDLSASTSDPDTDVDIPSSPIEYESVLDVSASEGPQDLSATM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AER7</accession>
    <name>G3AER7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 9">Vacuolar protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_58897</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Vacuole membrane</location>
            <topology evidence="1 9">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the beta-catenin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373104.1">
        <property type="nucleotide sequence ID" value="XM_007373042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AER7"/>
    <dbReference type="FunCoup" id="G3AER7">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AER7"/>
    <dbReference type="GeneID" id="18872666"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58897"/>
    <dbReference type="eggNOG" id="KOG4224">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021483_0_0_1"/>
    <dbReference type="InParanoid" id="G3AER7"/>
    <dbReference type="OMA" id="LEIVQCR"/>
    <dbReference type="OrthoDB" id="7537227at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071563">
        <property type="term" value="C:Myo2p-Vac17p-Vac8p transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071561">
        <property type="term" value="C:nucleus-vacuole junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071255">
        <property type="term" value="P:Cvt vesicle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051656">
        <property type="term" value="P:establishment of organelle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061724">
        <property type="term" value="P:lipophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071562">
        <property type="term" value="P:nucleus-vacuole junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000425">
        <property type="term" value="P:pexophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903044">
        <property type="term" value="P:protein localization to membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031503">
        <property type="term" value="P:protein-containing complex localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034517">
        <property type="term" value="P:ribophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045156">
        <property type="entry name" value="Vac8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47249">
        <property type="entry name" value="VACUOLAR PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47249:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0926">Vacuole</keyword>
    <feature type="repeat" description="ARM" evidence="8">
        <location>
            <begin position="2"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="8">
        <location>
            <begin position="43"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="8">
        <location>
            <begin position="84"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="8">
        <location>
            <begin position="127"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="8">
        <location>
            <begin position="168"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="8">
        <location>
            <begin position="209"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="430" mass="46629" checksum="418FE1915F271DB2" modified="2011-11-16" version="1" fragment="single">MGGLEPLIRQMMSTNIEVQCNAVGCITNLATQDDNKSKIAKSGALIPLTKLAKSKDIRVQRNATGALLNMTHSGENRQELVNAGAVPVLVSLLSNDDADVQYYCTTALSNIAVDEVNRKKLASTEPKLVGQLVNLMDSPSPRVQCQATLALRNLASDSGYQVEIVRSGGLPHLVQLLTCNHQPLVLAAVACIRNISIHPLNEALIIEAGFLKPLVGLLDYNESEEIQCHAVSTLRNLAASSEKNRTALLAAGAVDKCKELVLKVPLSVQSEISACFAILALADDLKPKLYESHIIDVLIPLTFSDNGEVCGNSAAALANLCSRVSTEHKSYILNNWRSPDEGIYGFLIRFLSSGSATFEHIALWTILQLLESNNTEINALIKENETILNGIKNLSAAQQQVQSSQINGEDQFDDPKVELFNLTQQILQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3AES6</accession>
    <name>G3AES6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58941</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373168.1">
        <property type="nucleotide sequence ID" value="XM_007373106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AES6"/>
    <dbReference type="FunCoup" id="G3AES6">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="GeneID" id="18872688"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58941"/>
    <dbReference type="eggNOG" id="ENOG502RXHE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103810_0_0_1"/>
    <dbReference type="InParanoid" id="G3AES6"/>
    <dbReference type="OMA" id="HEDFPYY"/>
    <dbReference type="OrthoDB" id="5330253at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018809">
        <property type="entry name" value="DUF2406"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28186">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28186:SF1">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10295">
        <property type="entry name" value="DUF2406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="188" mass="21307" checksum="CDCA671A95D9E279" modified="2011-11-16" version="1">MAIFRGKSAKKAPTKAGQIDVKISSKDAQQFKMHTANVHDPILKAVNEDQPFEQAARHDNRRPSYLSQDSGNLKDIFGNPIRQADMSNPTRERNERPLDTIRGFEYAITGDVGYRDQLESHRLGWGFHEDFPYYNLASGNQQRSNEFSRPVINFDQPVYQTGNYSASSLQGDGGKKAKKKRGLFGRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF09</accession>
    <name>G3AF09_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Eukaryotic translation initiation factor 3 subunit I</fullName>
            <shortName evidence="7">eIF3i</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Eukaryotic translation initiation factor 3 39 kDa subunit homolog</fullName>
            <shortName evidence="7">eIF-3 39 kDa subunit homolog</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TIF34</name>
        <name evidence="9" type="ORF">SPAPADRAFT_57892</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat STRAP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the eIF-3 subunit I family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372225.1">
        <property type="nucleotide sequence ID" value="XM_007372163.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF09"/>
    <dbReference type="FunCoup" id="G3AF09">
        <property type="interactions" value="1127"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF09"/>
    <dbReference type="GeneID" id="18872240"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57892"/>
    <dbReference type="eggNOG" id="KOG0643">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043845_0_1_1"/>
    <dbReference type="InParanoid" id="G3AF09"/>
    <dbReference type="OMA" id="VWFSHNG"/>
    <dbReference type="OrthoDB" id="24966at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071540">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3e"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000127">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03008">
        <property type="entry name" value="eIF3i"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027525">
        <property type="entry name" value="eIF3i"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877:SF1">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24805">
        <property type="entry name" value="EIF3I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="6"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="48"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="294"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="349" mass="38202" checksum="DB35345B1ABF65D3" modified="2011-11-16" version="1">MRPIKLMGHERSLTQVKYNREGDLLFSVAKDNAASIWYSSNGERLGTLEGHQGVIWSIDVDPETHLVATGGGDLAIKLWKVENGQCYYTWESPSPVRRVAFNSDGTKLLAVADQVMGHIGTISVFEINQENATLSNQKADPVLVISTAEGGSKVTVAGWSGDNKYIIAGHDNGSISKYDSQTGELVQSLQAHGIHNEEKVVSITDIQFAPEDKSYFITSSKDKTAVLIDVDTFEPLKLYKADAPMNTAAITPVKDFVILGGGQEARNVTTTAESQGKFEARFYHKIFQDEIGRVKGHFGPLNTVAVHPDGSGYSSGGEDGFIRVHTYDKSYYDFLFDAERTEKVDNAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF10</accession>
    <name>G3AF10_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dienelactone hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_57895</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372226.1">
        <property type="nucleotide sequence ID" value="XM_007372164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF10"/>
    <dbReference type="FunCoup" id="G3AF10">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF10"/>
    <dbReference type="GeneID" id="18872241"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57895"/>
    <dbReference type="eggNOG" id="ENOG502QQFN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054590_1_0_1"/>
    <dbReference type="InParanoid" id="G3AF10"/>
    <dbReference type="OMA" id="CATCFFP"/>
    <dbReference type="OrthoDB" id="58297at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47562">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47562:SF2">
        <property type="entry name" value="CARBOXYMETHYLENEBUTENOLIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dienelactone hydrolase" evidence="1">
        <location>
            <begin position="31"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="269" mass="30372" checksum="50B6A44A8C8C6E81" modified="2011-11-16" version="1">MLIEESYHDVKTSYGTTMRIFTYSPVIPNYPEVKFPGVIVYSEIYQVTGPVQRFAKDIAGQGFIVACPSIYHNFEGPEALAYDVEGTDKGNKYKIEKELESYDEDNKLTIEYLTSLPNCNGKIGATGMCLGGHLAFRAALDPRIKAAVCFFATDIHNHALGKGQNDDSLKRCGEIQGEIVMIFGCKDNHVPLPGRDLIRSELRSKDVEMTFIEINDAQHAFVRDESSKGRYDPAITKNCFEWLFELFNRKLKLEYGDHDGKNVFIQDIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF28</accession>
    <name>G3AF28_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Galactokinase</fullName>
            <ecNumber evidence="3">2.7.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Galactose kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">GAL1</name>
        <name evidence="16" type="ORF">SPAPADRAFT_57927</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Galactokinase is a key enzyme in the galactose metabolism where it catalyzes the conversion of alpha-D-galactose to galactose 1-phosphate. Can also induce the transcription of the gal genes in response to the organism being challenged with galactose as the sole source of carbon.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>alpha-D-galactose + ATP = alpha-D-galactose 1-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13553"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28061"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58336"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:13554"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GHMP kinase family. GalK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.6" evidence="3"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372244.1">
        <property type="nucleotide sequence ID" value="XM_007372182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF28"/>
    <dbReference type="FunCoup" id="G3AF28">
        <property type="interactions" value="1110"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF28"/>
    <dbReference type="GeneID" id="18872250"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57927"/>
    <dbReference type="eggNOG" id="KOG0631">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017814_6_0_1"/>
    <dbReference type="InParanoid" id="G3AF28"/>
    <dbReference type="OMA" id="CKISCEV"/>
    <dbReference type="OrthoDB" id="187738at2759"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004335">
        <property type="term" value="F:galactokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000056">
        <property type="entry name" value="GAL1p Galactokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000705">
        <property type="entry name" value="Galactokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019741">
        <property type="entry name" value="Galactokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019539">
        <property type="entry name" value="GalKase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006206">
        <property type="entry name" value="Mevalonate/galactokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00131">
        <property type="entry name" value="gal_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457:SF7">
        <property type="entry name" value="GALACTOKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457">
        <property type="entry name" value="MEVALONATE KINASE/GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10509">
        <property type="entry name" value="GalKase_gal_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000530">
        <property type="entry name" value="Galactokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00473">
        <property type="entry name" value="GALCTOKINASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00106">
        <property type="entry name" value="GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="7 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Galactokinase N-terminal" evidence="15">
        <location>
            <begin position="28"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="14">
        <location>
            <begin position="123"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00055546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF10509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW34832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="321" mass="35233" checksum="344B1BF72D1F07FB" modified="2011-11-16" version="1" fragment="single">MSSLVPTFTDLSFYTHDNKSRYNDLVTKFKKNFPESSVEFFARSPGRVNLIGDHIDYNFFPVLPMAIEVDVVAAVGVNDSNQIVITNTDEKDFPKQVIEIPSDPKVSVTIDKAQHGWGNYFKCGLIVAHKYLLEKGHNGKVKGLNIIFDGTVPTGGGLSSSAAFCVASTLAILHANGVKELSKSDLTKITVVSEHYVGVNTGGMDQCASVYGETNKALLIQFKPELKGTPFEFPIKNLTFVITNSLQVANKHETAPIHYNLRVVEMGIASDLLAKKLGLKDLPQDSNIDSSSLRGVMDGYYSTLNQEKWDGNDIDIGIERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFF0</accession>
    <name>G3AFF0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_53322</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372351.1">
        <property type="nucleotide sequence ID" value="XM_007372289.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AFF0">
        <property type="interactions" value="896"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFF0"/>
    <dbReference type="GeneID" id="18871775"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53322"/>
    <dbReference type="eggNOG" id="KOG0384">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_29_2_1"/>
    <dbReference type="InParanoid" id="G3AFF0"/>
    <dbReference type="OMA" id="WVQIRDD"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030874">
        <property type="term" value="C:nucleolar chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140002">
        <property type="term" value="F:histone H3K4me3 reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140750">
        <property type="term" value="F:nucleosome array spacer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000182">
        <property type="term" value="F:rDNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000729">
        <property type="term" value="P:DNA double-strand break processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000104">
        <property type="term" value="P:negative regulation of DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902275">
        <property type="term" value="P:regulation of chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001178">
        <property type="term" value="P:regulation of transcriptional start site selection at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17993">
        <property type="entry name" value="DEXHc_CHD1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000007">
        <property type="entry name" value="Chromodomain-helicase-DNA-binding protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000130">
        <property type="entry name" value="Chromodomain-helicase-DNA-binding protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041150">
        <property type="entry name" value="Cdh1_DBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056302">
        <property type="entry name" value="CHD1-2/Hrp3_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025260">
        <property type="entry name" value="CHD1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF14">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18196">
        <property type="entry name" value="Cdh1_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13907">
        <property type="entry name" value="CHD1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23588">
        <property type="entry name" value="HTH_CHD1_Hrp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01176">
        <property type="entry name" value="DUF4208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="12">
        <location>
            <begin position="166"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="12">
        <location>
            <begin position="265"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="369"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="673"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="18"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1174"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1211"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="57"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="87"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1261"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1279"/>
            <end position="1290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW34939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1410" mass="162696" checksum="CB9D8F5FB49781CA" modified="2011-11-16" version="1">MVKLYIPEEAALNPELYGLRRSHRERHGPAIVDSDSDEEEAAAAPSRRKRKKNVNDDYNDDFDDASEDGFDEDEDDEDDDFIDSSTRKSKTTSRKHSHKKKKVMSSASSVPQEIRFSSRNNKQVNYAVDYDDDDDLLESEPEFEDDDENDDYYYYQQANEQPEDERGVDLVMDHKLNEENAELTNDPKLDYLFKIKWTDASHLHNTWEKYQDLKDFKGFRKVENYIKQFIIYDQEIRNDPLTTKEDLEVMDIERERRRDEQDEYTQVERIVDSQRIENDLGENKLQYFVKWKRLYYDECSWEDGEEIAKIAPEQVSKYQQRLKSKILPNLSANYPISQRPRFEKLVKQPVFIKNGELRDFQLTGLNWMAFLWSRNENGILADEMGLGKTIQTISFLSWLIYARRQNGPHLVVVPLSTMPAWQETFELWAPEVNCVYYLGNGEARKTIREYEWYTPNGKPKFNVLLTTYEYILKDRNELGSIKWQFLAVDEAHRLKNSESSLYESLKGFKVANRLLITGTPLQNNLKELASLCNFLMPGKFNIEQEIDFESPDAEQERYIKDLQKKIQPFILRRLKKDVEKSLPSKTERILRVELSDIQTDYYKNIITKNYAALNAGNKGSQISLLNIMSELKKASNHPYLFDGAEERVLAKAGSASRENILRGMIMSSGKMVLLEQLLTRLKKEGHRVLIFSQMVRMLDILGDYLSIKGYQFQRLDGGIPSAQRRISIDHFNAPDSKDFIFLLSTRAGGLGINLMTADTVIIFDSDWNPQADLQAMARAHRIGQKNHVSVYRFVSKDTVEEEILERARKKMILEYAIISLGITDANSKKTKNEPSSSELSQILKFGAGTMFKENDNQKKLEDLNLDDVLSHAEDHITTPDLGESNLGSEEFLKQFEVTDYKADVEWDDIIPEEELIKLKDEEKRKADEEYLQQQIAMYSRRKATVRKFENGSSVPSDEDIDDDEERIGRRRKAGDHQLSEKEIRGIYRSILRLGDLSGKWEQLVEEGSITNKNPVLVKHAYNEIINTSRQLVKEEEARRAQALSELEKKAKEQRDKPLAPGEPNPTALWIAKKKEKKAVLFEYQGVKNINAELVLNRPVDMKLLEKLVPKDHPLSFELPRPPKSVQAWSCDWSAQDDAMLLVGVYKFGYGSWVQIRDDPVLGLQNKIFLENAPNSKEKPAEGEEANSKASKKVPGAVHLGRRVDYLFTLLGDEDTSNGGSSTPVPRKKVVRKPKMETPGATPAPSKTKKAIKSETPEVSFRKIKPKKPSSLHKHTSPPADKHGHGHDHDHDHKHHHHHKEKAESRDLEYDSMDEDGCKSAMHPMAKSLHRLHKGSKGLEKHEWAKILRNELLTIGDFIESEARRGDATKAEKLKKHLWSYTGLYWPSVVPSRKIREMYTRIKAQSKASSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFH4</accession>
    <name>G3AFH4_SPAPN</name>
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        <recommendedName>
            <fullName evidence="6">4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_58087</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="619300.G3AFH4"/>
    <dbReference type="GeneID" id="18872315"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58087"/>
    <dbReference type="eggNOG" id="KOG3244">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061241_0_2_1"/>
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    <dbReference type="OMA" id="YYERHFH"/>
    <dbReference type="OrthoDB" id="4249at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03111">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007715">
        <property type="entry name" value="Coq4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027540">
        <property type="entry name" value="Coq4_euk"/>
    </dbReference>
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        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922:SF7">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ4 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00081568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="263" mass="30085" checksum="A944314EBA6E6EC8" modified="2011-11-16" version="1" fragment="single">MLRNNSVKAIAQSQRIVKRPFVVAAAANALLGSFEFYKNIDLATKMDKGQLHYEDANAQFNKKRTDQPFFTRGEPEYPGHVPLYLHEKLLMFLGSSLGAYLHPERNEFIVALGESAAVTPVLRSIQTTMLNDPVGRQILRERPRMTSTSLDLDHLRALPSNSIGGTYVSWLDRENCSPDTRVPVRYIDNEELAYIYQRYRECHDFYHAITGLPITIEGEIAVKVFEFMNIGMPMSGLGSLFAPLRLKKSQKKRLYDVYYPWAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFJ4</accession>
    <name>G3AFJ4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein BNI4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_64184</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007372333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFJ4"/>
    <dbReference type="FunCoup" id="G3AFJ4">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFJ4"/>
    <dbReference type="GeneID" id="18874963"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64184"/>
    <dbReference type="eggNOG" id="KOG4339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_254113_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFJ4"/>
    <dbReference type="OMA" id="FTHNKIP"/>
    <dbReference type="OrthoDB" id="5563016at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12751:SF18">
        <property type="entry name" value="PHOSPHATASE AND ACTIN REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12751">
        <property type="entry name" value="PHOSPHATASE AND ACTIN REGULATOR PHACTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="880"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1070"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="229"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="317"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="379"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="394"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="474"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="584"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="606"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="901"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="942"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="998"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1038"/>
            <end position="1053"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1202" mass="132301" checksum="CC2DC52FA3DEA529" modified="2011-11-16" version="1">MTTNNSTSFDEFFSDDNFYSASITNSTNNHVNNLRNSIHIKYSASSRSIRDSLLEPNSTTTTTKAQNQANTRTSFMKASPSIKALENILNEKSKGWTNPNAAMTELKEEEEEEEDQEKDKLSSSNLLAPSFVYHNNETHSVQTFETAHESINKPEPKAHEPARRSVSTEDDFGYSTDETPVLQQPPTFQNFTPHSNNSPQLRFIETAGQVAAPKTDSSKENVNTNIAITTASSSNGKNSTNGTNKETGNKSSNWASGLNSKNTNNRDQENYSNTPRFIKMPNYPPSPPPPPPPQSYKSLVSSQLDSPVSFPPPPTMSALVSSPSAPSLTGMNASPALSNHQKYPLTNSPLYTTNNNSQNIPRVSTSTAITAQRPKQQHKQSSSSVSSQSIFSKANSTKGSNITDTPTELKKPKSMLDIPNSPREPPVTKSPHQRSNSSFSLNFKKAATYANPMDKPATPKPLNRKSQTFDDLKSFTQQQQSSSSAAPEKKKFSFKSLFKSKSKNHALNEQTREVQPPAEPKKIRSKSFSTSNIAMFNEVEEKPQKPSFINVFKKNRSSDNLAKIGSNAEKSSEPVPQPPAQTRTSKDVESFDSRPSKAHSANMIREVSDDDVRVVTDEEPTAIHEGSDEEEYDSKNLLHNPPRKLTGGNKFGEEMTFSIPPLLTDSPLGSPFQIDLDTSPRLGVVSKTPIDSLVSSGKNSPGPSGKNLEANLLGEALFPKSLNPQEVESIVTLERSMSMKSVKSKRSSFVNGNNDEHVVQYNGPVHTPGGISRSNSILKNSTSWRNNLSQELASVDGKLASANTSAVKPENAIFAASNNDSMVDDLLEFSDFIDVDNLSFSNSPKYITRPGSATPVQASPLEDRFPVQASSPLAERFPKEEKAKPIIPEIVTTDTSKDDTASPNISESQTPSPSMEHVMPKSSLEEEPEFEEKHSGLTNTITEKRDDKIEEEKKERDEKDEDSNVLEQSPILETAIRSGSAKFNNRPISMSFKGLNAPSFSGKLNQQTLRNSESHQSFTISIGDDESSSVGGGFGSSDGEDDDDDDDEDDNVSFDNEDEVFHLTQAPVPPVVARTTTPPPLPPKPRFRPGSMIPPAVKFSHNKIPSISDQSSINSASSPRSITSMMRWKKTSPVNPKMNGVRFSSRIILYDTYNEEEYDRHPDTATCNQLTPILAQQIKEELNTFKSEMDIHVESRCYTHFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFY0</accession>
    <name>G3AFY0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_69449</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372530.1">
        <property type="nucleotide sequence ID" value="XM_007372468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFY0"/>
    <dbReference type="STRING" id="619300.G3AFY0"/>
    <dbReference type="GeneID" id="18875271"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_69449"/>
    <dbReference type="eggNOG" id="KOG0134">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012153_2_1_1"/>
    <dbReference type="InParanoid" id="G3AFY0"/>
    <dbReference type="OMA" id="DGYANDW"/>
    <dbReference type="OrthoDB" id="72788at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02932">
        <property type="entry name" value="OYE_YqiM_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044152">
        <property type="entry name" value="YqjM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43303">
        <property type="entry name" value="NADPH DEHYDROGENASE C23G7.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43303:SF4">
        <property type="entry name" value="NADPH DEHYDROGENASE C23G7.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="7">
        <location>
            <begin position="47"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="412" mass="45407" checksum="8B4E3BD4B9D6A33D" modified="2011-11-16" version="1">MTIVREVKPSDDAKPSNVVSYYTPEQPIPAGTFIPPEDNPNQQPPGLFKPIQIGNMTIQNRVGVSPMCIFSADSQFEATPFHLVHYGSIAVRGPGIVIVESTAVSPEGGLSPHDLGLWNDNQAEKLRPIVDFIHSQSQKCAIQLCHAGRKASGQPLFLHLEQLADESVGGWPESVVGPSPIEFRPNGNLVRPRELTTSEIKRIVQDFGKAAKRAVDIGFDAVEIHGAHGYLINQFCSELSNQRTDEYGGSFENRIRFALEVIDEVKKSINNESIPIFLSVCASDNSLDKKGWVVEDTMKLVDHVIGHGVQLLDISSGGNCYNSISRSRLNDDHTLPPHVEIARKVKRHVGNKAVVACVGKLERDPVFTNKCIEDGDFDLALIGTPFLKNPGTVWNIADQLGVRWMRYRFSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFZ8</accession>
    <name>G3AFZ8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Inositolphosphotransferase Aur1/Ipt1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_58326</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372549.1">
        <property type="nucleotide sequence ID" value="XM_007372487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFZ8"/>
    <dbReference type="FunCoup" id="G3AFZ8">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFZ8"/>
    <dbReference type="GeneID" id="18872402"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58326"/>
    <dbReference type="eggNOG" id="ENOG502QPKA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047580_1_0_1"/>
    <dbReference type="InParanoid" id="G3AFZ8"/>
    <dbReference type="OMA" id="PMAPPWF"/>
    <dbReference type="OrthoDB" id="5784at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070916">
        <property type="term" value="C:inositol phosphoceramide synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006676">
        <property type="term" value="P:mannosyl diphosphorylinositol ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03386">
        <property type="entry name" value="PAP2_Aur1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026841">
        <property type="entry name" value="Aur1/Ipt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052185">
        <property type="entry name" value="IPC_Synthase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310:SF8">
        <property type="entry name" value="INOSITOLPHOSPHOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14378">
        <property type="entry name" value="PAP2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="370"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="394"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Inositolphosphotransferase Aur1/Ipt1" evidence="6">
        <location>
            <begin position="242"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="459" mass="53291" checksum="4330D21C20BC3AAF" modified="2011-11-16" version="1">MTLSLVARPFIFLYQLLSRIFWSGLNKKPIWRLILNFWIGFSTVFIWLLIFKNAGLIPHSIRPPIHVKLPVIMDHYMFSNMVGSVFMILCLSSFSYLLFVKYYRPNNCQNLTKYLPILNKRESIEIREESISSESSNSSDLEDHFGLDSFALDSTTSTIEPLSFIEPEDHVPPLEFFRTLSEAEITQRESETNAKIIDHLRATHSYQPRNCWYFAPPLLFFLSWGILNIDYWFKDPINTQKDLLAWFSYVICHITVPIVTAVWLYVFHAPGAVKLYGLTLGIQNICGVLTHLVFPNAPPWFVHMYGENKEANYEMPGYAAGLIRVDIALGTHLHSKGFHASPIVFGAIPSLHSAMAVMTFFFVSYYARWTAVKVASFLFVVIQWWATIYLDHHWRVDLLIGLSYALFWFTIVKNVRFGLNRVDEQFIKSRLRFDFAHGSTMGMRVFRNTRLQKFFDPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG19</accession>
    <name>G3AG19_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosome assembly protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Elongation factor-like 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_131598</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372570.1">
        <property type="nucleotide sequence ID" value="XM_007372508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG19"/>
    <dbReference type="FunCoup" id="G3AG19">
        <property type="interactions" value="818"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG19"/>
    <dbReference type="GeneID" id="18869593"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131598"/>
    <dbReference type="eggNOG" id="KOG0467">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_3_1_1"/>
    <dbReference type="InParanoid" id="G3AG19"/>
    <dbReference type="OMA" id="FARCDIQ"/>
    <dbReference type="OrthoDB" id="364892at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01681">
        <property type="entry name" value="aeEF2_snRNP_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04096">
        <property type="entry name" value="eEF2_snRNP_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01885">
        <property type="entry name" value="EF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16268">
        <property type="entry name" value="EF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000006">
        <property type="entry name" value="Elongation factor like GTPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1430.10:FF:000002">
        <property type="entry name" value="Elongation factor like GTPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000746">
        <property type="entry name" value="Ribosome assembly protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056752">
        <property type="entry name" value="EFL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF3">
        <property type="entry name" value="ELONGATION FACTOR-LIKE GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25118">
        <property type="entry name" value="EFL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="16"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW35158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1033" mass="116685" checksum="78AFABBDDC951300" modified="2011-11-16" version="1">MKIPPETVNKLQSDASCIRNICILAHVDHGKTSLSDSLLATNGIISQRMAGKVRYLDSREDEQLRGITMEASAISLYFKVMKMKEGEDNPEIKEHLVNLIDSPGHIDFSSEVSTSSRLCDGAVVLVDVVEGVCSQTVNVLRQCWIDKLKPLLVVNKIDRLITEWKLSPLEAYQHISRVIEQVNSVIGSFYAGDRLEDDMNWREAGAIGEFIEQSDEDLYFMPEKNNVIFASAIDGWAFSINTFTKIYSKKLGFSQNALSKTLWGDFYLDMKNKKIIPGKKLKQGTNLKPLFVSLILEQIWAVYENCIIERNQEKLEKIIEKLGARVTPRDLRSKDHKNLLNLIMSQWLPLSHALLGAVIEYLPSPIKAQSERIDKILQETIYSSVNAEKSKLVDVDFARALKECDSSNPEKHTLAYVSKLLSIPNEELPKDISSKGELTADEIKERTRIARELARKASEAAALAVDQKADTEDYSKPKKDAFEWEFEEEDFEEEEEEETHESNETLVGFTRIYSGSFTIGQKVTVIGPKYDPALPNSHPTNQDQITTVEIKDLFLIMGRELVRMQTVPAGNIVGVVGLDQAVIKNATLCSPHVDDEYPYINFASTSTLIHNKPIMKIAVEPTNPLKLAKLEQGLDLLSRADPVLEWYIDSESGELIVCVAGELHLERCLKDLEERFAPGCEVTVKEPVIPFREGLVSDKISTLYEDEESGEEDEDEDDGEIVDLEFEVSPLPLEMTQFLINHENSISEIVNSKHEIEEVKQEFLEKFTNVVAEHPTYGFDGASDFINHIIAFGPKRIGANIFVEAKGQNQFRHVLSNLDPPQHRFEYENNVLNGVQLALNEGPLANEPLQGVMITLKRAVKNEINEDKLVNPGRIIVQARDLIHKRMLQKSPRLFLAMYTCEIQAAAEVLGKVYAVVQKRGGAIISEEMKEGTPFFTIVARIPVIEAFGFSEDIRKKTSGAASPQLVFDGYDVLDIDPFWVPHTDEELEELGEFAERENVARRYMNNIRRRKGLFVDEKVVKNAEKQRTLKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGV3</accession>
    <name>G3AGV3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_131353</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372038.1">
        <property type="nucleotide sequence ID" value="XM_007371976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGV3"/>
    <dbReference type="FunCoup" id="G3AGV3">
        <property type="interactions" value="548"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGV3"/>
    <dbReference type="GeneID" id="18869566"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131353"/>
    <dbReference type="eggNOG" id="KOG0379">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005472_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGV3"/>
    <dbReference type="OMA" id="CSDESFQ"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244:SF471">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT BETA 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244">
        <property type="entry name" value="KELCH REPEAT DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="415"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="659"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="802"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="489"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="594"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="722"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="419"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="659"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="810"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="871" mass="100037" checksum="5E839104CA991DF7" modified="2011-11-16" version="1">MFQRSPQAQRSPQPIQAHRAQFGHVANSVTVPPSATSPWNRFKLFDSPFPRYRHAAASISSEKNEIFLMGGLKEGSVFGDTWKLVPMENHQGEVVNFTAKNLEILNHINPPARVGHSAVLCGNAFVIYGGDTVDTDANGFPDNNFYLFNTNNCKYTIPTHILNKPNGRYGHTIGVVSLTNSSSRLYLFGGQLENDVFNDLYYFELNSFKSPQASWELVEPANNFKPPPLTNHSMSIYQNQIYIFGGIYNNELVSNDLWIFNVEHNKWSKIDASGYIPKPVNEHSSCIVNDKLYIYGGNDFKGIIYSSLYVLDLNTFVWSKLIDMGEVNGPGPRCGHSMTFLPRYNKIIVMGGDKNDYVDNSDNFEMYEEYRENVVGTMIFQLDLNEIDHFLTSDPNKATVDKRIATRSMSALEEDYDASLQSPPNITRESQEPPTIKELPEPVSKESSVGVINEPPFDHLKDREPSFVDVPAAEVHHESIKDVSVKKIIAELNSEIQQLRKSTKEQMEIATEKITFLENENKSLKDSNRDFDYYQKQINDKDQLIEELKGNIKPEDESVTKFKLDSLESNNKLIYVTQENNKLKEKLEQFEPFMNNHIMELDKFQKLIKNQEDKISQLTNQIKSEESLHKEINDWKVKFDNLSLEFNNYKLAHDDSEEEIMENGDDDVTTETTRSVSSKKDISNQLENLIHLWNVKKTPEPTPEENPTVAQLQRQVDELLRISKENDQSTEVETLHKQLEEKTAALEKFEENYKQALQSVNNTSKALKLNEEEFEHQKKLLEKLTKENNELKLFKKASKRISSRSGTPDELTEESNEADDDDDEGISNAHLNIKIKDLEADLYIIRQERDQLKDHVISLQKELYLVKNEHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH46</accession>
    <name>G3AH46_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SRPBCC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_48467</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372889.1">
        <property type="nucleotide sequence ID" value="XM_007372827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH46"/>
    <dbReference type="STRING" id="619300.G3AH46"/>
    <dbReference type="GeneID" id="18871226"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48467"/>
    <dbReference type="eggNOG" id="ENOG502S64G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069867_4_1_1"/>
    <dbReference type="InParanoid" id="G3AH46"/>
    <dbReference type="OMA" id="NWNPFFV"/>
    <dbReference type="OrthoDB" id="509124at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07822">
        <property type="entry name" value="SRPBCC_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36166">
        <property type="entry name" value="CHROMOSOME 9, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36166:SF1">
        <property type="entry name" value="SRPBCC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="18088" checksum="887F47A9A68B84C7" modified="2011-11-16" version="1">MTTKLETSIEINAPVFKVRNFILNYSNHCNWNPFFVSFEKLTNKNRKELAIGDQLKIEVVYHGTSKVMTIYPTVIVNNFEQLKWKGILGFEWLFYGVHTLKYESLEHGTRTRLIHTEEFGGCLATLFTWLGITEKTELSFNDMNEALKDEIESLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH52</accession>
    <name>G3AH52_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein L22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_69713</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372892.1">
        <property type="nucleotide sequence ID" value="XM_007372830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH52"/>
    <dbReference type="FunCoup" id="G3AH52">
        <property type="interactions" value="293"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AH52"/>
    <dbReference type="GeneID" id="18875283"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_69713"/>
    <dbReference type="eggNOG" id="KOG1711">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081667_0_0_1"/>
    <dbReference type="InParanoid" id="G3AH52"/>
    <dbReference type="OMA" id="WVQLADK"/>
    <dbReference type="OrthoDB" id="416470at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="entry name" value="Ribosomal protein L22/L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047867">
        <property type="entry name" value="Ribosomal_uL22_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_uL22_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501">
        <property type="entry name" value="CHLOROPLAST 50S RIBOSOMAL PROTEIN L22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL22M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="Ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW35480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="287" mass="33369" checksum="D34C12E51F4A912B" modified="2011-11-16" version="1">MNRLAFYIRPLVRPQPVAWTAARSLSQTTPLFSLFGNVTSPKTTDKAEQEQKGQEILNSEGEIDIDKITVENDTDLQKFHAERNKKDRISIDKYITPLKLELFNKVVEEHGFYKNGQVVQHDGKTFKLNLTPKEIELLEPSVYLESTRIKSSMKKATQVNRFVRGLDVKTAINQLHFNPKKMSTELEKLLKEGLEQSTKLGLNPDGMYIHSLWTGSDGQWPWRMDFKGRGRGGIILHRYVHLKCILKSEQTKKRLAWEKQQKQLASKPKMFLVNEPLNFSVRPWYKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHD7</accession>
    <name>G3AHD7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_148516</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ORC5 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373685.1">
        <property type="nucleotide sequence ID" value="XM_007373623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHD7"/>
    <dbReference type="FunCoup" id="G3AHD7">
        <property type="interactions" value="872"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHD7"/>
    <dbReference type="GeneID" id="18870788"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_148516"/>
    <dbReference type="eggNOG" id="KOG2543">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028223_2_1_1"/>
    <dbReference type="InParanoid" id="G3AHD7"/>
    <dbReference type="OMA" id="QLRRWHG"/>
    <dbReference type="OrthoDB" id="365981at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020796">
        <property type="entry name" value="ORC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047088">
        <property type="entry name" value="ORC5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048866">
        <property type="entry name" value="ORC5_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12705">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12705:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14630">
        <property type="entry name" value="ORC5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21639">
        <property type="entry name" value="ORC5_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orc1-like AAA ATPase" evidence="7">
        <location>
            <begin position="17"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="ORC5 lid" evidence="9">
        <location>
            <begin position="226"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 5 C-terminal" evidence="8">
        <location>
            <begin position="315"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="490" mass="56344" checksum="FE9392E1FEA40877" modified="2011-11-16" version="1">MSLKAEESVDLKAQVKERGYEIDLLNAFINRDPTLSSDAVIVHGYKSVGKTLTVTSFLDALNVKYSRINCDECVSKKILLQRCFNRIRKDSGVQFAEGFSKADYLRYGRLGENFSTFISALEQFIRETEYSEHHVLILDRFDQCMEATDELFASFLKLREQSMIPNITIVVIISGDDPREVATLATPHIYFRAYTEDQVIRILQETPLCKFNMEDLDGTTAGYEFWCQYVKVIVDLFFQYCGSDMTILKDISMRLWDRFIEPVKSGELKVTEFVKVYKQNVDLFTSDNIISNSAVRDFKTLEEEKQIENGNVEDLPLHSKFFLLASYLASYIEQRNDLQKFSKVKGVKYKKRASTTVSKRGHLLKEDIDTRLLSPNFVDLERILAILSVIYRNYAPSLNRSDKDELLYIDDEIIVNEEKKEIEKAKFTLTSNIDLNNQIATLFSLGLLSKSASSDILGARLRWKCNINWPTAEGLAKLLNFPIAEFLNDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHG6</accession>
    <name>G3AHG6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_59548</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373714.1">
        <property type="nucleotide sequence ID" value="XM_007373652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHG6"/>
    <dbReference type="GeneID" id="18872968"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59548"/>
    <dbReference type="HOGENOM" id="CLU_870345_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHG6"/>
    <dbReference type="OrthoDB" id="10530893at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="371" mass="44617" checksum="C50C090AE3BA60EB" modified="2011-11-16" version="1">MEPRVYLMYLMNCEPPSEITNHHFHFVKVENDEQLLYDLIHRAEIEYAPEYIIKYQDHYTWNSKYDINKTSTGDLSDITMDLYELESNINWNCSSYGYAFKLTLIPFFVPHDSNFIFPPGVDPSWFEIVPVHNDTHLIKHLLTTVRGDQQNYLQLTRTGKLIYRDLKKSFADCQVFPCDSRSINRAIDTMVENMISSFRWPGPDYSNYERCRIEPLAKRIFEGMDQEQLRGYMEFPVWRDQKLIIVLFVSDYWLFRKLKKERNITIDEMFQICFSWHDYYYRCHHNHIEIMWHYKNNYTLVFENGKLELQTGFYEERDHNFELKCDFTSENMKLIKNKIESRHREALASLPSNVFVDSENELIKSCMNCGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHM7</accession>
    <name>G3AHM7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial</fullName>
            <ecNumber evidence="7">1.3.5.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Iron-sulfur subunit of complex II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">SPAPADRAFT_59621</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="7"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373775.1">
        <property type="nucleotide sequence ID" value="XM_007373713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHM7"/>
    <dbReference type="FunCoup" id="G3AHM7">
        <property type="interactions" value="717"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHM7"/>
    <dbReference type="GeneID" id="18872994"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59621"/>
    <dbReference type="eggNOG" id="KOG3049">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044838_0_2_1"/>
    <dbReference type="InParanoid" id="G3AHM7"/>
    <dbReference type="OMA" id="DGQYFGP"/>
    <dbReference type="OrthoDB" id="1696654at2759"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045273">
        <property type="term" value="C:respiratory chain complex II (succinate dehydrogenase)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000007">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1060.10:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase iron-sulfur subunit SdhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050573">
        <property type="entry name" value="SDH/FRD_Iron-Sulfur"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004616">
        <property type="entry name" value="PRK05950.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921:SF29">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] IRON-SULFUR SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE IRON-SULFUR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13534">
        <property type="entry name" value="Fer4_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="18" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="14" id="KW-0249">Electron transport</keyword>
    <keyword evidence="16" id="KW-0408">Iron</keyword>
    <keyword evidence="17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="22">
        <location>
            <begin position="46"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00016766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="EGW34191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="266" mass="30519" checksum="8E611F06DCD3C204" modified="2011-11-16" version="1">MIRSIIPIIRQPQQLQFGLRALATAAAQQSPRLKKFQIYRWNPDTPEVQPKLQTYEVDLNNCGPMVLDALLKIKNEQDPTLTLRRSCREGICGSCAMNIGGRNTLACLCRIEPDTSKDLKVYPLPHMFVVKDLVPDLTHFYKQYKSIQPYLQRDTNPADGRENLQSIEDRAKLDGLYECILCACCSTSCASYWWNQQQYLGPAVLMQAYRWLIDSRDEYTSNRKVMLQNSMSLYRCHTIMNCTRTCPKGLNPGKAIAEIKRLLAFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHR7</accession>
    <name>G3AHR7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 12">Coatomer subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Beta-coat protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_135572</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Golgi apparatus membrane</location>
            <topology evidence="1 12">Peripheral membrane protein</topology>
            <orientation evidence="1 12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle</location>
            <location evidence="12">COPI-coated vesicle membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373815.1">
        <property type="nucleotide sequence ID" value="XM_007373753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHR7"/>
    <dbReference type="FunCoup" id="G3AHR7">
        <property type="interactions" value="1295"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHR7"/>
    <dbReference type="GeneID" id="18869902"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135572"/>
    <dbReference type="eggNOG" id="KOG1058">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006949_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHR7"/>
    <dbReference type="OMA" id="IYKNFDW"/>
    <dbReference type="OrthoDB" id="10261439at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000430">
        <property type="entry name" value="Coatomer subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011710">
        <property type="entry name" value="Coatomer_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016460">
        <property type="entry name" value="COPB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029446">
        <property type="entry name" value="COPB1_appendage_platform_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10635">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10635:SF0">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07718">
        <property type="entry name" value="Coatamer_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14806">
        <property type="entry name" value="Coatomer_b_Cpla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005727">
        <property type="entry name" value="Coatomer_beta_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="13">
        <location>
            <begin position="17"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer beta subunit C-terminal" evidence="14">
        <location>
            <begin position="662"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer beta subunit appendage platform" evidence="15">
        <location>
            <begin position="801"/>
            <end position="928"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR005727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF14806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW34231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="943" mass="105545" checksum="696D34A982E50A0D" modified="2011-11-16" version="1">MGDVGYTLIYEPNSAIKTSVNEFKTLLEKGKDEVKIDTMKKILITILNGDPMPDLLMHIIRFIMPSKNKELKKLLYFYWEVCPKLDESGKMRHEMILVCNAIQRDLQHPNEYIRGNTLRYLNKLKEPELLETLVPNVRQCLEHRHAYVRKNAVFALHSIHKVSDHLAPDADELIYRFLYEEADAVCKRNAFVCLGDLNRDAALQYIQDNISVIETLDPLLQLAFIEFIKKDSLDNPNLNQQYAQLLTEIVESSSNVVVYEAANTLSLLTSAPSSILLVGTKFVELATKESDNNVKIITLERINELHKKHPGVLQELSLEILRVLSSQDLDVRKKALDVTLKFITSRNVEDVVKLLKKELQAASSSNDEKAAEYRQLLINAIHQMAIKFVEVAADVIDLLLDSIANLNTTAAYEVITFVKEVVEKFPNLRESILARLIDALPFVKSGKVLRGALWIIGEYALEQSLVQLSWKYIRSSVGEVPIIAAEKKSLNAENEEDEHSDNKVKRGPVILPDGTYATESALTAEVHDSDDASKTPIRKQIMAGDFYLGAVLSSTLVKLILRLQKLGTSEKIMNALKAEALLIMVSILRVGESSLVSKKIDEDSADRILSYIKILNDEEDVDTINTGFLEDTKDAFRAQIESAEVKKQQALAKDFHDNAEQVDDAIVFRQLDKNTRKSKANVDDVVAASGGNVKKENLSSRLNKILPLTGFSDPIYAEAFVKVHQYDVVLDVLLVNQTTTTLRNLSVEFATLGDLKVVDKPATANIGPHGFHKVQTTIKVTSADTGVIFGNIVYDGQHSNESTIVILNDVHVDIMDYIKPATCSETQFRKMWNEFEWENKITIKSQMDSLKDYMDELMKGTNMNCLTPGAVIGEECQFLSANLYSRSSFGEDALANLCIEKQSDGPIIGHVRIRSKGQGLALSLGDRVASISKKNKKVALARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHY2</accession>
    <name>G3AHY2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Transaldolase</fullName>
            <ecNumber evidence="4 10">2.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TAL1</name>
        <name evidence="11" type="ORF">SPAPADRAFT_59721</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the rate-limiting step of the non-oxidative phase in the pentose phosphate pathway. Catalyzes the reversible conversion of sedheptulose-7-phosphate and D-glyceraldehyde 3-phosphate into erythrose-4-phosphate and beta-D-fructose 6-phosphate.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:17053"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Carbohydrate degradation; pentose phosphate pathway; D-glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D-ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transaldolase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.2" evidence="4 10"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373880.1">
        <property type="nucleotide sequence ID" value="XM_007373818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHY2"/>
    <dbReference type="FunCoup" id="G3AHY2">
        <property type="interactions" value="704"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHY2"/>
    <dbReference type="GeneID" id="18873044"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59721"/>
    <dbReference type="eggNOG" id="KOG2772">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047470_0_1_1"/>
    <dbReference type="InParanoid" id="G3AHY2"/>
    <dbReference type="OMA" id="FATIKKY"/>
    <dbReference type="OrthoDB" id="2015515at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00414"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004801">
        <property type="term" value="F:transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00957">
        <property type="entry name" value="Transaldolase_TalAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000088">
        <property type="entry name" value="Transaldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00492">
        <property type="entry name" value="Transaldolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001585">
        <property type="entry name" value="TAL/FSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004730">
        <property type="entry name" value="Transaldolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018225">
        <property type="entry name" value="Transaldolase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00874">
        <property type="entry name" value="talAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683">
        <property type="entry name" value="TRANSALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683:SF18">
        <property type="entry name" value="TRANSALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00923">
        <property type="entry name" value="TAL_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01054">
        <property type="entry name" value="TRANSALDOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00958">
        <property type="entry name" value="TRANSALDOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0704">Schiff base</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="323" mass="35399" checksum="FF514E3078725A4F" modified="2011-11-16" version="1">MSSNSLEQLKATGTVIVTDTGEFEQIAKYTPQDATTNPSLILAAAKKPEYAKVIDVAIDYAKDKGSSPKEKAQIALDRLLIEFGKNILEIVPGRVSTEVDARLSFDKEATIKKALELIELYKTQGIDKDRILIKIASTWEGIQAARELEAKHGIHCNLTLLFSFAQAVACAEAGVTLISPFVGRILDWYKASTGETYTAETDPGVVSVKGIYNYYKKFGYKTIVMGASFRNTGEIKALAGCDFLTVAPKLLEELYNSTEAVPKQLDAAAAKSQDIEKVTFVDDEAAFRFALNEDAMATEKLSQGIRAFGKDAVTLLEQLEKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI84</accession>
    <name>G3AI84_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_48782</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373237.1">
        <property type="nucleotide sequence ID" value="XM_007373175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI84"/>
    <dbReference type="GeneID" id="18871267"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48782"/>
    <dbReference type="HOGENOM" id="CLU_687296_0_0_1"/>
    <dbReference type="InParanoid" id="G3AI84"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="248"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="401" mass="47742" checksum="D235078938A3AD76" modified="2011-11-16" version="1">MLTIGRKNGITNREEPLATLLTNDISAKSLKEFFIAVKEKIVFRDKQLLVVTNGMAAISTNIKYLSADAQEVYSTTHMKQLLEHTANKSSKSNQRALDQIVKQIIEVAEADTKHDADTRWAQLLEQQHEKAYLKNLWNLRSRWCRSYIGNHSKQLDLSNAIVTCIHRKIEARRWFEWEHVYYLLKHMSQTYEEYAKTKSDIINKIVPRFVTITEYQQIQDINQNIIGLMQDPDINGDTYTVEDKENLYFVKFDSDDITCTCQKFHDTQSWCNHIVRVIYPENDNAKLSIRALDSDLAEPEYYSCVEYDSIFHYLNPYIDRLNKYKNEIKLLKEYYREKSEYLDRIQETRHLKRIFSEETQFLMKEIAQKKKEQNNISDPRSLIKRSRYYEDPHFSEEYPFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIJ2</accession>
    <name>G3AIJ2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_59072</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373291.1">
        <property type="nucleotide sequence ID" value="XM_007373229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIJ2"/>
    <dbReference type="STRING" id="619300.G3AIJ2"/>
    <dbReference type="GeneID" id="18872757"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59072"/>
    <dbReference type="eggNOG" id="KOG0791">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001645_9_1_1"/>
    <dbReference type="InParanoid" id="G3AIJ2"/>
    <dbReference type="OMA" id="GHKWWIA"/>
    <dbReference type="OrthoDB" id="6058203at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18533">
        <property type="entry name" value="PTP_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF561">
        <property type="entry name" value="PROTEIN TYROSINE PHOSPHATASE 36E, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="2">
        <location>
            <begin position="91"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="3">
        <location>
            <begin position="245"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="358" mass="41172" checksum="34469F80F7A95AAB" modified="2011-11-16" version="1" fragment="single">MNPFEENSANLFRLRNIPADTSVLPEWIKQTIFDKNGEASTNKINEDFYKLEKLEQKRLVSALSLLPSGLLSSPLGEIPPKVSCGIEYGHKNRYKDIFLYDHSRVKLNDHEDDNIENYINASYVKPNLDFLGQLNNSNELNYVATQGPLDGTMGDFWKCIYNLGTVLILSLTDEVENGIVKCSPFWKSGIYKSCQDSMTVNLIDEERDENIIIRSFSIRKSNGLDHKVLQVHLVNWPDMGISDPREIIKVVKLKQDLLNLNLTDSKFPTLVHCSAGCGRTGTCCTIDVMINVLQQCRDLPYDPIFDIVNDFRKQRISMVQALRQYYLIYEVLLIWLSQEQQPLDRLIDRDIIRKFISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIK8</accession>
    <name>G3AIK8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">TRS120 targeting complex component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_133774</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373307.1">
        <property type="nucleotide sequence ID" value="XM_007373245.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AIK8">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIK8"/>
    <dbReference type="GeneID" id="18869766"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133774"/>
    <dbReference type="eggNOG" id="KOG1953">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002231_1_0_1"/>
    <dbReference type="InParanoid" id="G3AIK8"/>
    <dbReference type="OMA" id="EHSRDRM"/>
    <dbReference type="OrthoDB" id="27962at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058565">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058567">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058568">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058564">
        <property type="entry name" value="TPR_TRAPPC9_Trs120"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013935">
        <property type="entry name" value="Trs120_TRAPPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058563">
        <property type="entry name" value="Trs120_TRAPPC9_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512:SF5">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26254">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_1st"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26280">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26282">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26283">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26251">
        <property type="entry name" value="TPR_TRAPPC9-Trs120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08626">
        <property type="entry name" value="TRAPPC9-Trs120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trs120/TRAPPC9 N-terminal" evidence="4">
        <location>
            <begin position="5"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 TPR region" evidence="5">
        <location>
            <begin position="374"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 first Ig-like" evidence="6">
        <location>
            <begin position="664"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 first Ig-like" evidence="6">
        <location>
            <begin position="776"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 third Ig-like" evidence="7">
        <location>
            <begin position="1026"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 fourth Ig-like" evidence="8">
        <location>
            <begin position="1206"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="321"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="754"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="758"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF26251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF26254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF26282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF26283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1344" mass="151394" checksum="732386E8BAC32B58" modified="2011-11-16" version="1">MSDKYNFIAPAIVKVLVVPINNCTKANFTRYLQLLHSNVSEVRLLDITPNSDLSHFNPASFPNGRIFIEFTTTTIDQESIFLHDFEPFRKTFIVIAVGTYSEELNAQQHLDAIKTRFPTTIMANSIMFDTPSVQVESLNKLNPDVFYHDGTTNKLTALETIFCDICGNFLNELDSYASSFANITLRSPVSITDSHVLTKAINKAQKRISLGGSTSFKVSFNGNSAPTSMETKSKTHLRHLGRQRKLMGNFYLLSGKYHDALQSFIEGLQALRKCDDYLWLASALEGMAVSIVLLQFIGSQYTLPSQILCPILQVTKSKMNSLSDPTLSPRKSSIDSQSSKITTNLSPRTSFSGATNGFTLNSLTSTPDLNTVALPELLNVVMSRALSFYDLSTNDFEDMVPDIVYVESILRNIRLMIGFHLSNGEMASTDLENIIRGKKTESATYQGYFYRSDILAEIDKIFQLQLVDLPIIDQCRIYSVLASMYSDLGMMRKRAFILRTLLVGLLPDLEKQSSTTTPGQIQSIREIVDFLFVLYGINSEPEVSAEAASFQSQSNWPALQIQFLKLCMKIGENINDKEFLLKLCTLLLTRYTHNLPEDDQIRLKEKVESLIFQSTIDKLSLSVPYWDPFMVRRAKFVNSKQRDELIPFNEYLENGVSQESTDSFFDPYQKTVEVSVFEKDKLLIKDDVNHLKITLQNPFAFEIEINDLEIATEGDVQVETIKSLIRAINPSPSQQQSLNNTIRASSMTKVRGLTKKASPVNSTSPNNFSSSSSQTSSVMVVPPNSTEQFLVAFRALHVGQLKIIGFRASIGPCKPQFFPIVDKELYAKSVKITDHSISEVPNTLDKVIANLKNASIESRATTRKLVLNVISRQPTLTLTDTSVTNGWIMLLDGEKFDFSIRLTNHSPVTINYLSFSFWDSTIEPLNNKLNSAGVQNTLDAAEMYEIEWALVKFKPFRIVNKDEIAEKYKTIGPGDDIEIDYEVTGRKGMDLSKIILDYAHKNSDDIPNSFVKHIHIPLNISVMPAIEVVGCDILPLFASSLQGLDVTYQHNPGNLNQVLQFISGLIESKKDVSDYCLFIIDLRNSWTDKLRINLKYSDSVEFEINDSIVPDKTSRFLIPIKRIKPNEIDFTKPIPSLRNRQFVKNYNLTEEAEKLMQKLFWLRDHILLKLSGSWSGSDDARHGTIDLRPLRLTAKNSSILVYDNILINHSICDESDTPLEKQGTQYCLQADEFYILKTTIVNNSESPITGIIRHLPIPRSITSSIHPTSVSFKAQLSIDRKILFNGVLQNKISDVPLQPGAKLELELSFVIIERGEFEWATVLEVLAPESAHYVGHEPTYIMAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIZ7</accession>
    <name>G3AIZ7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Class E vacuolar protein-sorting machinery protein HSE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Class E vacuolar protein-sorting machinery protein hse1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_59944</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the STAM family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374093.1">
        <property type="nucleotide sequence ID" value="XM_007374031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIZ7"/>
    <dbReference type="FunCoup" id="G3AIZ7">
        <property type="interactions" value="361"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIZ7"/>
    <dbReference type="GeneID" id="18873145"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59944"/>
    <dbReference type="eggNOG" id="KOG2199">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010104_2_0_1"/>
    <dbReference type="InParanoid" id="G3AIZ7"/>
    <dbReference type="OMA" id="QVYRDWW"/>
    <dbReference type="OrthoDB" id="10255964at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033565">
        <property type="term" value="C:ESCRT-0 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904669">
        <property type="term" value="P:ATP export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016237">
        <property type="term" value="P:microautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903319">
        <property type="term" value="P:positive regulation of protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21386">
        <property type="entry name" value="GAT_Hse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11805">
        <property type="entry name" value="SH3_GRB2_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16978">
        <property type="entry name" value="VHS_HSE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050670">
        <property type="entry name" value="STAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45929">
        <property type="entry name" value="JAK PATHWAY SIGNAL TRANSDUCTION ADAPTOR MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45929:SF3">
        <property type="entry name" value="JAK PATHWAY SIGNAL TRANSDUCTION ADAPTOR MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VHS" evidence="15">
        <location>
            <begin position="12"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="14">
        <location>
            <begin position="215"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="184"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="370"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="336"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="151"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="372"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW34509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="437" mass="49993" checksum="0BE4BECF8DAB5556" modified="2011-11-16" version="1">MSPTLSTLINKATDPTLTADNWQYILDVCDKISSDPETTTKEAIRELTFKLNSKDANTILRSLSLLVSIAENCGSRIKQEIATKTFLQDNLIRKLSDRKLHVTVKYKIVEVIEQLYNTFKTDPSLKPMTDAYKKVQSDYKQYFKSAPSKPAKQERTQQDKQREDDELQRILKLSLQEYEREQTMKKVSLQEKPLPDDPTSNQPNQSPPNEPQTVATVSKVRALYDLISYEEDELSFRKGDVITVIESVYRDWWRGSLPNGKIGIFPLNYVTPIVNKSNKEIAQEVSIENNLLSEQKKIDKLLSILSSQNVQNINEDEITQLYNEIIPLRPQLGKFIDKYNVRKEELMVLNNQLNNEVKSYNELLDQSIARRAQQQHSPQQQFTAPQMAPYPTGAGTGTGVVPPPPQANFQPSYEYQNLQQQPTSAGFGNGRPQYPYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ40</accession>
    <name>G3AJ40_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_149605</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; biotin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374136.1">
        <property type="nucleotide sequence ID" value="XM_007374074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ40"/>
    <dbReference type="FunCoup" id="G3AJ40">
        <property type="interactions" value="212"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ40"/>
    <dbReference type="GeneID" id="18870831"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_149605"/>
    <dbReference type="eggNOG" id="KOG1401">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_4_3_1"/>
    <dbReference type="InParanoid" id="G3AJ40"/>
    <dbReference type="OMA" id="GGCFMHG"/>
    <dbReference type="OrthoDB" id="425114at2759"/>
    <dbReference type="UniPathway" id="UPA00078"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004015">
        <property type="term" value="F:adenosylmethionine-8-amino-7-oxononanoate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000041">
        <property type="entry name" value="Adenosylmethionine-8-amino-7-oxononanoate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00834">
        <property type="entry name" value="BioA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005815">
        <property type="entry name" value="BioA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00508">
        <property type="entry name" value="bioA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004624">
        <property type="entry name" value="PRK05964.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005940">
        <property type="entry name" value="PRK07986.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42684">
        <property type="entry name" value="ADENOSYLMETHIONINE-8-AMINO-7-OXONONANOATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42684:SF17">
        <property type="entry name" value="ADENOSYLMETHIONINE-8-AMINO-7-OXONONANOATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="438" mass="49165" checksum="9AF09F8055E00C72" modified="2011-11-16" version="1">MNPKISDSIPQEDKDFDKQHIWHPYSSMSKPLPCYHVKSASGVRLYLDSGQSLIDGMSSWWCAIHGYNHQELNDALVKQVNLMSHVMFGGITHDPAIQLCRHLVELTNPKLECVFLCDSGSVSVEVALKQAIQYWDSKRQPEKKKFLSIKRGYHGDTFGAMFVSDPENSMHSIYTSYGPDNIFAEAPQIGFDDEWNEADIEDFKRKIEKHHKIIAAVILEPILQGAGGMRMYNPQYLKRVRELCDKYDVLLVLDEIATGFGRTGKLFAQEHANICPDIMCVGKAITGGYLTLAAVISTRSVANVISGGRTGCFMHGPTFMGNPAACAVSVKNLEILRTGAWKSQVDTIEKILLKKFKPLMESGHRKIRQIRVLGAVGVIEMKNPIDVALYQRRFVELGVWVRPFGKLLYIMPPYIISEDDLNHLVYSLISVATDESLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ51</accession>
    <name>G3AJ51_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_65035</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 14">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373392.1">
        <property type="nucleotide sequence ID" value="XM_007373330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ51"/>
    <dbReference type="FunCoup" id="G3AJ51">
        <property type="interactions" value="1327"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ51"/>
    <dbReference type="GeneID" id="18875011"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65035"/>
    <dbReference type="eggNOG" id="KOG1376">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015718_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ51"/>
    <dbReference type="OMA" id="YMASCIL"/>
    <dbReference type="OrthoDB" id="1662883at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045298">
        <property type="term" value="C:tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000008">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 14" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="15">
        <location>
            <begin position="50"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="16">
        <location>
            <begin position="249"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW33808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="448" mass="49840" checksum="8FB4B406FFB8D74C" modified="2011-11-16" version="1">MREVISINVGQAGCQIGNACWELYSQEHGIRPDGYLQEGLTRPKGGEEGFSTFFSETGSGKYVPRALYVDLEPNVIDEVRTGLYKDLFHPEQLIAGKEDAANNYARGHYTVGREILDDVLDRVRRMTDQCDGLQGFLFTHSLGGGTGSGLGSLLLEQLSIDYGKKSKLEFAVYPAPQVSTSVVEPYNTVLTTHTTLEHADCTFMVDNEAIYDMCRRNLDISRPNFSSLNNLIAQVVSSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYAPVFSKTRATHEANSVSEITQSCFEPGNQLVKCDPKAGKYMATCLLYRGDVVTRDVQNAVAQVKAKKTVQLVDWCPTGFKIGICYQPPTATSGSELAAASRAVCMLSNTTAIAEAWRRIDKKFDLMYSKRAFVHWYVGEGMEEGEFTEAREDLAALERDYIEVGTDSFQEEEEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ84</accession>
    <name>G3AJ84_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cell division control protein 45</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_134703</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC45 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373425.1">
        <property type="nucleotide sequence ID" value="XM_007373363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ84"/>
    <dbReference type="FunCoup" id="G3AJ84">
        <property type="interactions" value="659"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ84"/>
    <dbReference type="GeneID" id="18869845"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134703"/>
    <dbReference type="eggNOG" id="KOG2475">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005871_3_0_1"/>
    <dbReference type="InParanoid" id="G3AJ84"/>
    <dbReference type="OMA" id="EDCFMEA"/>
    <dbReference type="OrthoDB" id="10258882at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902977">
        <property type="term" value="P:mitotic DNA replication preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003874">
        <property type="entry name" value="CDC45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10507">
        <property type="entry name" value="CDC45-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10507:SF0">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 45 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02724">
        <property type="entry name" value="CDC45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="177"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="466"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="228"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="178"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="472"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="718" mass="82147" checksum="87BFB549504541F5" modified="2011-11-16" version="1">MYINPPQYSRAFQEIKRTSLSHSTCKLVIFVSCLDIDSLCAAKILSLVLRKELIQYQLIPVVGYSDLKSHYAKLDSDVSNIFLIGCGAMLDLEGFFELNPEEFVEERQEQVTNDLDLDLKKDTAIPMKRKIYVMDGHRPWNLDNLFGSAMIVCFDDGFIDANLKTEKLAYTNLMDQLQGEEEDEEESESDMTDEDDDEFEGDTDVDEQSDEELIINSQDSDDTVSRKRKLQEIKMKKIKKQRKREKSENQDKIQQYYNQGTAIITANSITIYALLSAIGETSLENLWLSIIGTSSLDNQFPELYDKLHPLLRDEVLRINPSNEPASVSGSTKTADSTSLSIEKDYHLFLLRHWTLYDSFFYSSQVNSKLNLWTEDGKKKLHKMFAKMGVSLAVAQQKWMYMDSRVKRQLPGIFHHYLPLYGLEGIVRQGFIRTFGFTGQLSAMECVEALTALLELDKRFLTSNGMDTQANENEQDPDAEEEEEAHDEEERIEKQLQKKEKVWVNNFWSAWDALNMGNTLQIGATTQKTSFKKPKGFELLFQGLEHAKQIQQVIFRTGMSLLERKLIKNLKLYRFCVLNDGSIPDLHIFNNPLMLSKLGSWVAENLTELDFTNESHTLKPLILASLDVDSDSYLVMGIAPKYPRGMNNATRAKLAQEQARDENGETQMTRLNTFSVAFQQLSNTSGAKIRIDSFDSSVIEIRKDDLSPFLERLTLSGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJM8</accession>
    <name>G3AJM8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_49025</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373513.1">
        <property type="nucleotide sequence ID" value="XM_007373451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJM8"/>
    <dbReference type="GeneID" id="18871296"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49025"/>
    <dbReference type="eggNOG" id="ENOG502TFR8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023521_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJM8"/>
    <dbReference type="OMA" id="CKVPNYY"/>
    <dbReference type="OrthoDB" id="4091342at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="611"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="611"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="775" mass="89907" checksum="D5AB3CA741AED27B" modified="2011-11-16" version="1">MSKVIFNDIIPIIPTHSYSTKRTQLIFSEEAAATTTPVNKTNFAVPIFQIQQQDGKLQDYVLIKHLSQLWNYPSSYQLITKLINNTVLTIHQFKKTDEAINHQLNQYSLIEVKDIKFELFYIALEDIYSVINNKHVFYQSLTAKPRVKSQAVAISASGDTSINSSNAQSNKPDEKAQEEQEEEDEDESDESDEEDEEDEVDDEDVKTPKPSHHIHKYKKNESTVLTSSASNDDIITVSQAFPQFGIVDSVVQFNHAQFNSLNPLTKLNYYKTLPSSSLHKFLPNNKLTFSERELLLTSHNYYSNDSEDSKKQQQQIERKLFRKPIGKSKKHNIHVDPNTIDLNESVIPGQGYIQEFNINHICKVPNYYITSNHQPAASASTPDGLASPGQPAASIGKINMKKLKLNSTSNSSFLFNDNIKMSKNIQQLIFSNDSDNYHHAKYYYTKSYRGPGSGNYKDAALMNKINKIKLTNDPIKKIHKKKISTIKSRYNHSLKGLLHEKFNNQLVETVFAKQRKFTEDYSNLEMLHNNLQFNVLLNTYRHISNQTWKNYFKFKTTDFEQLKASQVEQREIELRKQAIENHNQWIEQEKNRQEQIKLILDQERAEFESLQREHIQKQKSIEEKKRQRQLEDTFNDPFISDSNKESAGEEEQYEALNQEFQQKQLELSKKFEAKKREFLAPIPPPVPVETPQFDIINRFSLPTIYPEIVKNLPLELRQESIEKDDVPSIKKPIRYISTYAQEGIKNPEFVTKVEMIKLPNANSVGWDNLRKFKHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJN2</accession>
    <name>G3AJN2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59317</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373517.1">
        <property type="nucleotide sequence ID" value="XM_007373455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJN2"/>
    <dbReference type="STRING" id="619300.G3AJN2"/>
    <dbReference type="GeneID" id="18872864"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59317"/>
    <dbReference type="eggNOG" id="ENOG502T02G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_863728_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJN2"/>
    <dbReference type="OMA" id="HQTNEIE"/>
    <dbReference type="OrthoDB" id="4092725at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="322" mass="36778" checksum="BFC9CBEFB90B2B95" modified="2011-11-16" version="1">MIPQNFNIVTNAIVFVLAVTGPWIWVRMHQTNEIEVLQQLLRDGLFPVTIPIAIQFAGDVSFTFPDLIEATQFQIDREVSHLTNSSLYIQLIDNFDDSSPSLYSIDLILNNENTLGISATSLKGYVFYTLDSVHSNDLPFVITQTILYHLIRPEIDLMNRPLTENFPDSLDIKLKFNVHNETVQHNVTEFMDKMKQKLVGISVMNWEVDNSLTTRDNATLSVKFSGQNSSIIISPESDPVAELSMILQEQLNFPKYPKHNIYLKIEAAYRIKTLQILQELTDKIRNKNPSQLLQNLVHQIISETSPSWIGYLTTARQLLDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJQ2</accession>
    <name>G3AJQ2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL29m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">54S ribosomal protein L4, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_59346</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373537.1">
        <property type="nucleotide sequence ID" value="XM_007373475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJQ2"/>
    <dbReference type="FunCoup" id="G3AJQ2">
        <property type="interactions" value="218"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJQ2"/>
    <dbReference type="GeneID" id="18872877"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59346"/>
    <dbReference type="eggNOG" id="KOG3331">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_872105_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJQ2"/>
    <dbReference type="OMA" id="IRTTMWR"/>
    <dbReference type="OrthoDB" id="270763at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038340">
        <property type="entry name" value="MRP-L47_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010729">
        <property type="entry name" value="Ribosomal_uL29_mit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21183:SF18">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL29M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21183">
        <property type="entry name" value="RIBOSOMAL PROTEIN L47, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06984">
        <property type="entry name" value="MRP-L47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="291" mass="34130" checksum="2A2973E41DF934EE" modified="2011-11-16" version="1">MNIIRRSLSSAAPAQAVARKLKFTGEIKLRAPIVPTHQNFDVNPNHPLWQFFPDKTTALRESEQLDLESREWTMNELRRKSFDDLHKIWYLVLKERNILAREVRLGEAVYADTSKFIELDEKLVKVQKRVKNTLLERQVAYERAQLLQEQQDSYLSEFKQRFINCEESESNDYFDKLIRLQYAFFGIKPDIQDFDIDHDINVKFAQGLSYIADIKLQRYLKLNPDLQFAPLNGVMEELPFLLNDTADAIEEVKALRESGQSVKLQKIQVLPFLRNAIENVLKVVENEQEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJU7</accession>
    <name>G3AJU7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Pyruvate decarboxylase</fullName>
            <ecNumber evidence="11">4.1.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PDC6</name>
        <name evidence="11" type="ORF">SPAPADRAFT_49085</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.1" evidence="11"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373582.1">
        <property type="nucleotide sequence ID" value="XM_007373520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJU7"/>
    <dbReference type="FunCoup" id="G3AJU7">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJU7"/>
    <dbReference type="GeneID" id="18871302"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49085"/>
    <dbReference type="eggNOG" id="KOG1184">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_0_2_1"/>
    <dbReference type="InParanoid" id="G3AJU7"/>
    <dbReference type="OMA" id="AQEISVM"/>
    <dbReference type="OrthoDB" id="308383at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004737">
        <property type="term" value="F:pyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000949">
        <property type="term" value="P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02005">
        <property type="entry name" value="TPP_PDC_IPDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07038">
        <property type="entry name" value="TPP_PYR_PDC_IPDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000024">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012110">
        <property type="entry name" value="PDC/IPDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047214">
        <property type="entry name" value="TPP_PDC_IPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047213">
        <property type="entry name" value="TPP_PYR_PDC_IPDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452:SF3">
        <property type="entry name" value="TRANSAMINATED AMINO ACID DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036565">
        <property type="entry name" value="Pyruvt_ip_decrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="10">
        <location>
            <begin position="35"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="9">
        <location>
            <begin position="469"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="514"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="545"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="547"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="634" mass="71194" checksum="0CEC2945497E0829" modified="2011-11-16" version="1">MTPVIETIQDGESSISPNNSGVCSPVSIPNEIHLGEYLFLRIAQANPKLRSIFGIPGDFNVDLLEHMYSPLIAAKDMKFVNCCNELNGAYTADGYARTIGGLSCLITTFGVGELSAINGIAGAFAEYVPVLHIVGVTTTKQAQQAKDGTEVLNYHHLVPSVNPFDSPNHDVYKNMVNGVSVIQETLGTDQELNVEKIDRVLKKIIQESRPGYLFVPADVSDILVPSVFLFMNPFNAEPKHKNSAKSIEVLDDVCDTILEKMYKSTKPSVLSDCLTSRFGFKEQLDQFVDLLPTSIAKLFTTNMARNVDETRENFIGVYHGKGSSDDGVRQQLENETDLLVTLGYFHNEVNTGAYSRNLTDIKDIIEVHPDYIKINHQVYHIKQKDGERLFTLGDLLSELTSRFDPTKFANHTPVPMYSFTPGAIYKQSSNDNYFIPQTKIIDHLNTWLKPNDLLIMETMSFSFALPDLKFPRNLQLLSANFYGSIGYALPATFGATMAINDMGSNRRIILIEGDGAAQMTIQELSSFIRYQGVLPNMPKIYLINNDGYTVERAIRGPYRSYNDINGHWKWTQLMETFGDSDRMRHDSVTLKNVDEFEGYFNGHKSSNNSRLQFYEIIAGKYDVPERFSKSLCKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJY1</accession>
    <name>G3AJY1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histone H2A.Z-specific chaperone CHZ1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Histone H2A.Z-specific chaperone chz1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_59452</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms a chaperone-bound H2A.Z-H2B complex that acts as a source for SWR1 complex-dependent H2A to H2A.Z histone replacement in chromatin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CHZ1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373616.1">
        <property type="nucleotide sequence ID" value="XM_007373554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJY1"/>
    <dbReference type="STRING" id="619300.G3AJY1"/>
    <dbReference type="GeneID" id="18872920"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59452"/>
    <dbReference type="eggNOG" id="ENOG502SCUM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126134_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJY1"/>
    <dbReference type="OMA" id="RTHYDDE"/>
    <dbReference type="OrthoDB" id="4026566at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019098">
        <property type="entry name" value="Histone_chaperone_domain_CHZ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09649">
        <property type="entry name" value="CHZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01082">
        <property type="entry name" value="CHZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone chaperone" evidence="9">
        <location>
            <begin position="90"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="22"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="71"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="114"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="148" mass="16861" checksum="D28FB30A8E90381F" modified="2011-11-16" version="1">MSKEEALKKVEEGEASEATEVEESKVEESAEAKEDDDSKKRSGDSDNKKKKRRRRQYDDDVPPESETKPEQEEEEGEEGSGDDDEPEEEADEEDDLLEIDEANIITTGRRTRGKVIDFAKAAKELDKEQGTIQEDGEEEEEEEDSEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK00</accession>
    <name>G3AK00_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59470</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373635.1">
        <property type="nucleotide sequence ID" value="XM_007373573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK00"/>
    <dbReference type="GeneID" id="18872930"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59470"/>
    <dbReference type="HOGENOM" id="CLU_2256569_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK00"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="104" mass="12300" checksum="E6E9E84337BBE004" modified="2011-11-16" version="1" fragment="single">MPDQIHNEYHHQLEFMFFFAFYAFIAKFGGVFGPSGNQLYESLSNEDQLNARYEIFDQFVEVARKHTSKRYKELCKTAEGRKKFDEIAKMFGFTKATKDVQIHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKF8</accession>
    <name>G3AKF8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Polyol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_60259</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374430.1">
        <property type="nucleotide sequence ID" value="XM_007374368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKF8"/>
    <dbReference type="FunCoup" id="G3AKF8">
        <property type="interactions" value="150"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKF8"/>
    <dbReference type="GeneID" id="18873300"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60259"/>
    <dbReference type="eggNOG" id="KOG0024">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_0_1"/>
    <dbReference type="InParanoid" id="G3AKF8"/>
    <dbReference type="OMA" id="LFCGHCA"/>
    <dbReference type="OrthoDB" id="5363962at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000721">
        <property type="term" value="F:(R,R)-butanediol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034079">
        <property type="term" value="P:butanediol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08233">
        <property type="entry name" value="butanediol_DH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161:SF23">
        <property type="entry name" value="(R,R)-BUTANEDIOL DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="8">
        <location>
            <begin position="32"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="7">
        <location>
            <begin position="206"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="361" mass="39074" checksum="882EF99893F42DB5" modified="2011-11-16" version="1" fragment="single">MTASTMKAIVYHDRGDVRYDPAFPEPNITRGDDVKIKVHYCGICGSDLKEYTDGPIFFSKKGTNNEISNLPYPQCMGHEISGEIIEVGKDVTNVVPGDKVVVEVTGTCFDRYRFPDSPGFDKPKCPSCHVGHYNACDYLGLTGLGFTDGGCSEYLVTAASKVIKFDENIIPMDVAALVQPIAVSWHAVRVSRFVEGQSALILGGGPIGLTTIFALKGHKVGHIVLSEPALARRQLAEKLGVEVFDPTGKSVEQCVTELRAMSPNGHGFNYSYDCSGVPATFETGLKTLNIRGTATNVAVWAHKSVSYFPMETTFSEKVVTGSICFVKEDFEDSIAAIQSGLISVDELKLLITSVIKLEDGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKQ0</accession>
    <name>G3AKQ0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acyl-CoA desaturase</fullName>
            <ecNumber evidence="11">1.14.19.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_65969</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Stearoyl-CoA desaturase that utilizes O(2) and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>octadecanoyl-CoA + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = (9Z)-octadecenoyl-CoA + 2 Fe(III)-[cytochrome b5] + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:19721"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="EC" id="1.14.19.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="11">Expected to bind 2 Fe(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.1" evidence="11"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374467.1">
        <property type="nucleotide sequence ID" value="XM_007374405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKQ0"/>
    <dbReference type="STRING" id="619300.G3AKQ0"/>
    <dbReference type="GeneID" id="18875073"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65969"/>
    <dbReference type="eggNOG" id="KOG0537">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1600">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027359_3_2_1"/>
    <dbReference type="InParanoid" id="G3AKQ0"/>
    <dbReference type="OMA" id="KSQLNWG"/>
    <dbReference type="OrthoDB" id="10260134at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004768">
        <property type="term" value="F:stearoyl-CoA 9-desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03505">
        <property type="entry name" value="Delta9-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009160">
        <property type="entry name" value="Acyl-CoA_deSatase_haem/ster-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015876">
        <property type="entry name" value="Acyl-CoA_DS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351">
        <property type="entry name" value="ACYL-COA DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351:SF31">
        <property type="entry name" value="DESATURASE 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000345">
        <property type="entry name" value="OLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00075">
        <property type="entry name" value="FACDDSATRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 11" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="11" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="3 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="14">
        <location>
            <begin position="408"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="189"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="503"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="19"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="189"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="202"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="503"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW32952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="528" mass="60124" checksum="A4206191F2A1BB14" modified="2011-11-16" version="1">MSDKPVRITTKVQKRKTHSNSPSAQSTSATTHAHQTTELIRKRHLIKRVNYANTVTTVVLPLIAIAYIFHNHQGIIPKNVTTLYFTCIYYNITMLCFTSGYHKYFAHNSFKINYSWLAKLFLIFGSSVGLGCAKWWAGLHRAHHHFVDDVEKDPYSIKRGFWFAHWGWVLWKPKNVAWFTEFIESEFSSDVDHEEEEDENGNGNGNGNGSGNGNGSGNDNENDKGNTFASHDDEHRDLLIWQEKTYLVWFAITTFIIPVFVTMFVCNDTAINGVIYPGILRMFLCQQSMLTPESICHTKRIQVTIPSQPFNDTNSSIDSQNPLISFLTYGQSQHNYHHEFPHDYRTHNSHFTYDPTKWFLVALDKLNLITNLSCTPTNLIIQLQIQQQQRYLNRIKSQLNWGTPISKLPKITPRDFKRIVNSPQNADRIYIVIQNIIHDITPFMDQHPGGVALLKASHGKDATKAFYGGVYGHSTAAQNLLATMRIGVLDDGNEEEVWRRVAREEGESGERRAAAAAVSGQYRTAEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKR0</accession>
    <name>G3AKR0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60296</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374478.1">
        <property type="nucleotide sequence ID" value="XM_007374416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKR0"/>
    <dbReference type="GeneID" id="18873326"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60296"/>
    <dbReference type="HOGENOM" id="CLU_1310782_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKR0"/>
    <dbReference type="OrthoDB" id="10508111at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="210" mass="24185" checksum="B479621A09597B95" modified="2011-11-16" version="1">MADNSLADQVEDLIKIVKSTLHLTNYQKHELTKLLTMTMEMIQVSRNQDYAKHSRLVDSYRQLVIQTISPQKHPSRSKRSSMFELHDEHYISNLSPPPSRTTTNTSMYRFPHEQQGLNTPTDESTIHNETGSDSEFDDERSNTVSLAHPRTTRPTIINRKLKHIEPDAHANPFTTSNLARTFNMPVTPHKQQSSATTTPLHSMNSYIRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKT9</accession>
    <name>G3AKT9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA replication licensing factor MCM3</fullName>
            <ecNumber evidence="12">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_54957</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="12"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374508.1">
        <property type="nucleotide sequence ID" value="XM_007374446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKT9"/>
    <dbReference type="FunCoup" id="G3AKT9">
        <property type="interactions" value="1110"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKT9"/>
    <dbReference type="GeneID" id="18871903"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54957"/>
    <dbReference type="eggNOG" id="KOG0479">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000995_6_0_1"/>
    <dbReference type="InParanoid" id="G3AKT9"/>
    <dbReference type="OMA" id="NVYPQED"/>
    <dbReference type="OrthoDB" id="1882346at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904931">
        <property type="term" value="F:MCM complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17754">
        <property type="entry name" value="MCM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000008">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008046">
        <property type="entry name" value="Mcm3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056575">
        <property type="entry name" value="WH_MCM3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF46">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23191">
        <property type="entry name" value="WHD_MCM3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01659">
        <property type="entry name" value="MCMPROTEIN3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="14">
        <location>
            <begin position="311"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="683"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="697"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="737"/>
            <end position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW32993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="851" mass="95141" checksum="9795CBF0AF7A6B73" modified="2011-11-16" version="1">MDDLTQTGDQPVDAIFGDRVRRFQEFLDRIDSTHGIDYRALIRDMLAKSKYRLNVSIDEIRDFDREFWNGLLNTPADFLPACERALRDTVLTIYDPNDPAFPDDFDFQNQQYYLSFKGAFGAHSVNPRSIDSSYLSKMVSIEGIVTRASLVRPKIIRSVHFADKTGRFYAREYRDQTTSFDPISTPAIYPTEDMDGNKLSTEYGYSTYRDHQKISVQEMPESAPAGQLPRSVDVILDDDLVDLAKPGDRVQIVGVYRALGGASNNSSSFRTVILCNSIYPLHARSTGVAQQEKLTDQDIRNINKLSKDKKIFDILSSSLAPSIYGFEYIKKAVLLMLMGGVEKNLDNGTHLRGDINILMVGDPSTAKSQILRFVLNTASLAIATTGRGSSGVGLTAAVTTDKETGERRLEAGAMVLADRGIVCIDEFDKMSDIDRVAIHEVMEQQTVTIAKAGIHTSLNARCSVIAAANPVFGQYDVHKDPHKNIALPDSLLSRFDLLFVVTDDVQPTKDRIISEHVLRMHRFIPPGLMEGEPIREKSSVTLAVGDDETNEQELLEQPIFEKFNALLHTGAQYSGDRKSKKTPTILSIPFLKKYVQYAKQRIKPVLTKGASDHIVSTYSSLRNDLIGNNQRNTAPITARTLETLIRLATAHAKVRLSKTVDVKDAKVAEELLRFALFKEVSKKSSKKRKTNTTTATEEQEEESESESDETDSEPEIIPTPTRRSLRTQIRQQRDSNTIEEESDDGGVGDELDHFHISRNQPLQDKHVPANTGAGTISSERFHTFQRIMARISRSGLFENEGGAAAYDSVVSAVNEGLAQEEVFDELEIQAAFKKMEDDNKFYISEGKIWKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKV4</accession>
    <name>G3AKV4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inosine/uridine-preferring nucleoside hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_150506</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IUNH family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374512.1">
        <property type="nucleotide sequence ID" value="XM_007374450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKV4"/>
    <dbReference type="FunCoup" id="G3AKV4">
        <property type="interactions" value="199"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKV4"/>
    <dbReference type="GeneID" id="18870868"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_150506"/>
    <dbReference type="eggNOG" id="KOG2938">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036838_2_0_1"/>
    <dbReference type="InParanoid" id="G3AKV4"/>
    <dbReference type="OMA" id="NVEHIHA"/>
    <dbReference type="OrthoDB" id="432381at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070635">
        <property type="term" value="F:nicotinamide riboside hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070636">
        <property type="term" value="F:nicotinic acid riboside hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008477">
        <property type="term" value="F:purine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045437">
        <property type="term" value="F:uridine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006216">
        <property type="term" value="P:cytidine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034355">
        <property type="term" value="P:NAD+ biosynthetic process via the salvage pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019358">
        <property type="term" value="P:nicotinate nucleotide salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006152">
        <property type="term" value="P:purine nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008655">
        <property type="term" value="P:pyrimidine-containing compound salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006218">
        <property type="term" value="P:uridine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02651">
        <property type="entry name" value="nuc_hydro_IU_UC_XIUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="entry name" value="Ribonucleoside hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023186">
        <property type="entry name" value="IUNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304">
        <property type="entry name" value="INOSINE-URIDINE PREFERRING NUCLEOSIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304:SF4">
        <property type="entry name" value="URIDINE NUCLEOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53590">
        <property type="entry name" value="Nucleoside hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="4">
        <location>
            <begin position="11"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="334" mass="37424" checksum="E3A5D8BF5631EB1F" modified="2011-11-16" version="1">MIQITNRPIPVWLDCDPGNDDVFAILLVLFHPRFNLLGISTVHGNAPLEMTTHNTLALLDLLQVYNVKVYEGSKRPLKIPPHYAFGVHGTTGLGGVTLPKKTINKPAKDMYYIEAMRSAICEHQGEICLVSTGTLTNISKLIAKYPEVKDKIRYIAIMGGAFNFGNVTPFAEFNIHTDPHAASSVIAEFGDKVILTPLNLTHTAVATEWVRKQIYNDVEGDNRRCSEIRKFFHDILMFYGQAYRENFGMTEGPPLHDPLALYSLLPFVDDAFEEYGYKYVQQPIKVVLDGEHQGETVVSKENTPGVYVGSEVDTKKFWQSVLVALKNAEVHIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL03</accession>
    <name>G3AL03_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SWIRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60364</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374561.1">
        <property type="nucleotide sequence ID" value="XM_007374499.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="G3AL03">
        <property type="interactions" value="1056"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL03"/>
    <dbReference type="GeneID" id="18873367"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60364"/>
    <dbReference type="eggNOG" id="KOG1279">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004447_2_2_1"/>
    <dbReference type="InParanoid" id="G3AL03"/>
    <dbReference type="OMA" id="MFISTTY"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000020">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032451">
        <property type="entry name" value="SMARCC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802:SF41">
        <property type="entry name" value="BRAHMA ASSOCIATED PROTEIN 155 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802">
        <property type="entry name" value="SWI/SNF COMPLEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16495">
        <property type="entry name" value="SWIRM-assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SWIRM" evidence="6">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="7">
        <location>
            <begin position="181"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="146"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="296"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="456"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="456"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="499" mass="56354" checksum="E0D5B84BA7B0BF99" modified="2011-11-16" version="1">MKKIHKIEKDSLPEFFDTNHPSKSPKIYVNYRNFMINSYRLNPNEFLTLTSCRRNLVGDVGTLMRIHRFLNKWGLINYQVSPQFKPGYAIEKLPNGQSVDLPYTGDYHVKFDSPRGLFPFDTSRIPPDRIDVNNLKKLLGTPTDNSNGIASAGSVADQNGNKHGLESDESSLSDHQRKKQKVDDGWTHDEYDALINAVKTFKNDWYKIADAVGTNKTFLKLPIEDRFNPIKDEASIKLLKYAPNYPVSSIDNPVLANLAFMTKLVDSDVAKAASEAARKAIEADIEKKVDEVYNKGQEVKSESQEEEQQISTEQNGSSEREPTAKDAISTTFGIIGARSHLFSSYEEREMHKIGATIVNHTLSKVDVKLNKIEELEKIYERERKHLAKQQEEVLLDRLALTKSTINVIKKLDSAIQLIEQNANINGESTFDKSNIGNILAEAKSLLYKPTRQGLASSTLTTTEDSNQPVKEEISSDPFQEDEYKPLSLKSPQNFKVWAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL52</accession>
    <name>G3AL52_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_137673</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374610.1">
        <property type="nucleotide sequence ID" value="XM_007374548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL52"/>
    <dbReference type="STRING" id="619300.G3AL52"/>
    <dbReference type="GeneID" id="18870065"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137673"/>
    <dbReference type="eggNOG" id="KOG1303">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009646_8_2_1"/>
    <dbReference type="InParanoid" id="G3AL52"/>
    <dbReference type="OMA" id="AICYTVC"/>
    <dbReference type="OrthoDB" id="655540at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF349">
        <property type="entry name" value="AMINO ACID TRANSPORTER TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="435"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="493"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="121"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="520" mass="57498" checksum="0BB475F15E10B882" modified="2011-11-16" version="1">MSTPTEVRPIPRRERRTSILDMGGPNSINNFASSYTRAQQYIGSTLIEQGGNLIEQLSRQPSVSNEEAVEFVTPDSYGPFRFQATNEVDEECAVDTEETSLLPELSRISTKRSSFTIITGNSTVPQTVFNSVNTLVGIAMLTLPFGFRLSGWLFGMLFMLFTAFCSNITAKYLGRILRQYHHLSTYGDIAHEFGGPYFSYFVTFFFIFDLTGASLTLIILFADSLSIVWPHIHALKIIIVGLIFLLSLLPLSILSLFSLLGILGTLCIIIIIVLCGFLSDQQPGSLIFPEATSMLPPAWKNLLFSLGIFMAPWGGHPVFPELYRDMRHPGKYSKSCNISFSTTFFLDLAIGALGYLMFGNTVDDSIIKTIMNNPHYPKYINKILCLLMGLLPLSKLPLVTKPIITSYENVFGLTPKYVVVDKEGQLADTYGFTRIVARAIFFSLLLALGLLFTSFGKLVSFLGSAICFTVCLALPLLFYMKLNGDNIGTIHKVFLKIGVVLSLSAAVIGTYSSLAMNVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL60</accession>
    <name>G3AL60_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_150740</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphatase 2A regulatory subunit.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374618.1">
        <property type="nucleotide sequence ID" value="XM_007374556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL60"/>
    <dbReference type="FunCoup" id="G3AL60">
        <property type="interactions" value="678"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL60"/>
    <dbReference type="GeneID" id="18870879"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_150740"/>
    <dbReference type="eggNOG" id="KOG2085">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012437_1_0_1"/>
    <dbReference type="InParanoid" id="G3AL60"/>
    <dbReference type="OMA" id="QYIDHEF"/>
    <dbReference type="OrthoDB" id="10264446at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032186">
        <property type="term" value="P:cellular bud neck septin ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070199">
        <property type="term" value="P:establishment of protein localization to chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051754">
        <property type="term" value="P:meiotic sister chromatid cohesion, centromeric"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000786">
        <property type="term" value="P:positive regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031107">
        <property type="term" value="P:septin ring disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031134">
        <property type="term" value="P:sister chromatid biorientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000372">
        <property type="entry name" value="Serine/threonine protein phosphatase 2A regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002554">
        <property type="entry name" value="PP2A_B56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE 2A PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A 56 KDA REGULATORY SUBUNIT GAMMA ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01603">
        <property type="entry name" value="B56"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028043">
        <property type="entry name" value="PP2A_B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="779"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="39"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="73"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="122"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR028043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="801" mass="90640" checksum="BA7B773B63EB0B35" modified="2011-11-16" version="1">MMKGFKNRLTRNKSQSSNKKSDKKSPEKEKVEKSEKRSSSPSSIGSGGSSSQQSSKNLLSGSSSTLNRTSSPNQSRNNSSAGSIKSATSNASMTSSPQGSPLRAGVVPPAITPPRITTTAAQTSSKPAIISGNSPFNGQSQVQQQQQQAHTQQNLPFTPTPTSPLTSSSSVVAAGASTGIPSTLHGTGSPFEIGKASHSQHAAQTHFQLTPNIASPNREPIDIDLIVTPKRHSSSRFEPPNADRYQEITKLPNFDEVLPEDQIQLFIQKIDQCNVMFDFSDPTFDIRGKEIKRITLQELIAFISNNRFTYTDEMYKHVVTMFKKNLFRPIPPPVNPVGELYDPDEDEPVSELAWPHMQAIYEFFLRFIESPDFQHQIAKQYIDHEFILRILELFDSEDPRERDCLKTTLHRIYGKFLSLRSFIRKSINNVFLQFVYETERFNGIGELLEILGSIINGFALPLKEEHKIFLVRILMPLHKVKSLSLYHPQLAYCIVQFLEKDPTLTEDVIMGLLRYWPKINSPKEVMFLNEIEDIFEVMEPNEFVKIQIPLFAQLSKCISSPHFQVSEKVLCYWSNEYFLTLITENAEVVLPIIFASLYELTNATQPGIENGIMQQKLQHFPNATTDANGNLIDSGLVLQDLQSGIIPSSNDPNHPHYNIFQVHASVNNRSTDINGNDMTEDEYYDSFSSMHNASANSMDEYGGFDDSNMLQHNSATSNWNRSIHSLAFGALKVFMDHNPVLYDHCTVLYHQSLEEQKQREVMRKEGWKRIEEYVKKIKEKQDHGAEDHTSENIEIVKKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM40</accession>
    <name>G3AM40_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pre-mRNA-splicing factor CWC22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60100</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374260.1">
        <property type="nucleotide sequence ID" value="XM_007374198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM40"/>
    <dbReference type="STRING" id="619300.G3AM40"/>
    <dbReference type="GeneID" id="18873219"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60100"/>
    <dbReference type="eggNOG" id="KOG2140">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1149587_0_0_1"/>
    <dbReference type="InParanoid" id="G3AM40"/>
    <dbReference type="OMA" id="TEGMRET"/>
    <dbReference type="OrthoDB" id="3938623at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050781">
        <property type="entry name" value="CWC22_splicing_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034:SF3">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC22 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MI" evidence="7">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="154"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="167"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="242" mass="28354" checksum="1986C38C88922C82" modified="2011-11-16" version="1" fragment="single">KTYSKFYGIIGEKMLDRPKYQAIFTTLFQDYYNNISNYEVNAIRNIGKFFGYLFSTDLLELNKVFNHISLNERDTNSQGRILLKFIFQGMIEELGMAELKKRLSEDYIKRDINGMFPVVDITWDDAEDLRFSINFFTAIGLGELTDEMRSVLSQLHPPEERGRSRSRSTSRSSSYSRSYSRSYSRSVSYSRSRSRSQSRSQSRSPRRSSRDPSPTRSFSRTPSREPLKRRQPSLSPPPKRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM49</accession>
    <name>G3AM49_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2</fullName>
            <shortName evidence="8">Oligosaccharyl transferase subunit OST2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_60106</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Endoplasmic reticulum membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the DAD/OST2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374269.1">
        <property type="nucleotide sequence ID" value="XM_007374207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM49"/>
    <dbReference type="FunCoup" id="G3AM49">
        <property type="interactions" value="749"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM49"/>
    <dbReference type="GeneID" id="18873222"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60106"/>
    <dbReference type="eggNOG" id="KOG1746">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111220_1_0_1"/>
    <dbReference type="InParanoid" id="G3AM49"/>
    <dbReference type="OMA" id="LVENDHA"/>
    <dbReference type="OrthoDB" id="445566at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003038">
        <property type="entry name" value="DAD/Ost2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10705">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10705:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02109">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005588">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="167" mass="18539" checksum="299446E8F4D3E654" modified="2011-11-16" version="1">MSKQEIKKRVAAAAAASSESGAHPVVPSSSVTTTLNELYSAINTTIADYTDSLTPRLKLIDSFLVFLVALGVLQFVYVLIVGNFPFNAFLAGFISCVGQFVLTVSLRLQINEKNKHKQSEKQTVDEDEEEETGKEETVFKFISPERAFGDYIFASLILHYIVIHFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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    </comment>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="254" mass="28255" checksum="CE62D0AB54A9B40D" modified="2011-11-16" version="1">MSEVKRTIRITTEQHILKDVPPVENYPMRQWNIQISMLDASGQEIPANILDKVTYTLHPTFANPIRTIKQSPFRVEEQGWGEFDIPISVHILGLAGKAGERKFQHDLNFYQEKYVNDHVISIPVSSTKNPALNKLLLESGSLPFDDGSAAGAKRKLEGNGSSSDLSKVKKSKSGTTAGAAVKGSVDLEKLANGLTKLNEDDLIVIVQMVTDNRTNEMNIKNDVESGEFTMDLYTLPESLLKSLWEYVKKHTTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
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                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="86" mass="9891" checksum="02CF14D6B3AA7C23" modified="2011-11-16" version="1">MTQESQASIADTYKQLNEAEKQAANLEKMLDNFESKLESILKEAQAISQEELKQVEEPKQEEPKQEEPKQEEQKQEEPTGDTQSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMH2</accession>
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    <protein>
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    <organism evidence="2">
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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    <dbReference type="Proteomes" id="UP000000709">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="227" mass="26766" checksum="A13C4F202A2804B2" modified="2011-11-16" version="1" fragment="single">MFLFRARRMPCGRITRRYVSRTSEQTQDPAHKEHINRVVSGAIRKREGKAVKHYQENYTLVDQYLKHHKKVLDIADNPKIVSSMFSRDSKLQTATYKNLEHILGSLSYTNVDMKSTILLISHSLLESISIEERLKSKGVPELHNPTNLKYAIDHIKESSWFNSLEQYRYKFHHYKSGQYLNYWKSDTTPAILGEDYIRFHSTVKDSSEYINRFDSAFDKYQKDSNHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMQ1</accession>
    <name>G3AMQ1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MIF4G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="6">
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007374948.1"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <begin position="36"/>
            <end position="246"/>
        </location>
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            <begin position="378"/>
            <end position="565"/>
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            <begin position="581"/>
            <end position="814"/>
        </location>
    </feature>
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        <location>
            <begin position="836"/>
            <end position="922"/>
        </location>
    </feature>
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        <location>
            <begin position="844"/>
            <end position="911"/>
        </location>
    </feature>
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        <location>
            <begin position="912"/>
            <end position="922"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW33495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1031" mass="119392" checksum="655C6BF324DAAA96" modified="2011-11-16" version="1">MSSELDNRRKELLDLNTKAWNGEHVYKVAAKLDSSLKKNTAFIKKIKTGVNSDQYKSVLKDIETVSIEKYLSEVIVNLVEALYKVSKSDDILAAIEIVSAFHQRFTTLFTPQLLIHVLVGISNPVRSHQSDIDEKEEQSRIVRQRNLLRLVGEFYIIGVFRVLKDCNKDSIPHELLLKFGKQASEPILIVVIKDLMNFELKSGNSLSVIQSFLKRFQNIICNDDNVLLSFEVRNVLVQIFSIYSKAVFDITVDLKKRVKQLTERNNKASIRTGRILEEIQVELDDVKSRYEKFKSTAEFLSDTLDIPPLNWNLDEEDTKQEPDSVVELVKSKSLNEDDLNGVWEDIKEKTFYTVIPSLGEIMDSTSMENSKSVSSKDGERIQDFLNRLEDVGANDLEQLVVEFNNLNLNNKATKNRIMRFFIETSNVSNLKYYARFLKINEINLSDLIEELITYLDKGFRHQLYQNKLNFKNILFFVELIKFKMVPIHLIFHKIRTLTLNISATNNIDILAVFYENVGRFLMNEPEYKDLMVEMIELLKEKSKKSNLKINDKLAINNLLLIVNPPANTKVQREKSTLSLKQQFIQRLIRVELDAKSVPVIIRLLNKLHIQTDEESCDTVLDCFAHPELVNYDNLPALAEVLQMYSKNHKKIVVYTIDTLIENIIRGLELNDYRMNRVRMSQIKLVAELYNKKIVQFKLLNDLLYRILCSGHPNNQPLPNNWEVEIDLPDNYFRIQLCCLLLLSIKSIIVDSDISPKKKSPAKQASDLKKKNEINKELLGVFTTFLQYYIFCKQTPLPVELQFKLTDLFAKYEDVSTVKRYETIREVVQKLQESIQTKKEVESQLMDDEDDDDNIADEELDYDEYIDPEDEDDSDGDDDDEEEEDDDDSSDDEGDDEDEDEDEDSDVENEVPEPERVRIEEAKKFSDDIDKEFQKIMIESYNISARPQQGGARGKLNMPVPRQVLVANEIKNNGNDSSKVSFGLLTRSGKKTNVKQLNLPSDNKFAESILKEKEHQRQDRQKIMNLVLQMDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMX7</accession>
    <name>G3AMX7_SPAPN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">SPAPADRAFT_61463</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Debaryomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61463"/>
    <dbReference type="HOGENOM" id="CLU_2980577_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMX7"/>
    <dbReference type="OrthoDB" id="10432045at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
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    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="58" mass="6153" checksum="F65524BC8E31964C" modified="2011-11-16" version="1">MAQGAPGSIAGSDFGIGDRRDVIRGKSISSNTDSSLGIEFQQRYTYSPCGDKDESIPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANF2</accession>
    <name>G3ANF2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_138616</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138616"/>
    <dbReference type="eggNOG" id="KOG3230">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904669">
        <property type="term" value="P:ATP export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:intralumenal vesicle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:protein retention in Golgi apparatus"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="190"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="16"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="233" mass="25913" checksum="8358A05BF6A77128" modified="2011-11-16" version="1">MSALFEWAFGKKLTPQERLRKNQRALEKTQRELSREVTKLQQQEKKLISDIKKSAKQGQISSAKIQAKDLIRTKSYISKFNSMKAQLQAISLRIQSVRSNQQMAMSMRDATRLLGGMNRSMNLPSLSRIAQEFAKENDMMDQKQEFMDDAIDDAMALDEDELGEEEQIDEILGKVLDEIGVDLNANLKDTPSAIHAGEQPESNARVAEAVGAGGGGGGADEDDLQARLDSLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANN7</accession>
    <name>G3ANN7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61636</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375842.1">
        <property type="nucleotide sequence ID" value="XM_007375780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANN7"/>
    <dbReference type="GeneID" id="18873982"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61636"/>
    <dbReference type="eggNOG" id="ENOG502RQCC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_474056_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANN7"/>
    <dbReference type="OMA" id="KIRFFEN"/>
    <dbReference type="OrthoDB" id="4026422at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="548" mass="62273" checksum="08282A76535AB75F" modified="2011-11-16" version="1">MSGQGESTLSPHEEDWSIISSCSDNEDDPSTAGFPDQDLDYSLSLSDRIKTNHVSDSVSNSILQVPELITSTESSKATVKINESEIHDYVESREDDDAAVATDAEIVEDDESQFTPSSSSDIKSQISIGSDQYINVAPKINFYENLSKFNDSIKQKSNEFYSNYAKVKMDQLNNYLADQHPEEIHEGEIDPTLNQSELKIEAEDSPGFESSAVSTPSSTPVAPPTIVLPYWKRCLLRGIDYVKDVLDCNSDYLYYYFVGITIGIFSLGFGVRYLWPAPVIDHPMTTMEKVNQFWDSIIYEEEVIPGNIFSFSKKKQKINKWVKYSSEFEISVFDATDKIFKNVWDKKSFREFSIRMNEFYISTNHTLYPLVAKAWENAKVMRQEARDNLEEWGKVGFENLDKYSRIGIGNLDKYGRLGWIKFRENGNNYMELVKEYGIKGWVRADEYSAIGYKYVVRGTERLLTHSARASNKLVVFMESFVSSGAEIGGNYVEKTRKAVKALLDSDANTRLKNSVGNELNLVMAEGKQAARLVRKQSELLFNELFGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANN8</accession>
    <name>G3ANN8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_51111</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375843.1">
        <property type="nucleotide sequence ID" value="XM_007375781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANN8"/>
    <dbReference type="STRING" id="619300.G3ANN8"/>
    <dbReference type="GeneID" id="18871517"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51111"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_506382_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANN8"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050329">
        <property type="entry name" value="GLI_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818:SF144">
        <property type="entry name" value="METALLOTHIONEIN EXPRESSION ACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818">
        <property type="entry name" value="ZINC FINGER PROTEIN ZIC AND GLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="155"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="54"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="192"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="244"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="461"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="16"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="244"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="279"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="465"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="538" mass="59022" checksum="E7DDF11AAF93927C" modified="2011-11-16" version="1">MGFELPGDRSRIFEGSSQSQPRPELEPSSSPRPTLPPIQQLTSGYPIAPIYAVLDGTPSNETSQPSGNVHSCESTPVPTGHTPEASISDATKPSLPKKYFCKICKQGFTRKHNMVSHELIHSSLKPHICAVCNLKFRRIHDLKRHEKLHMGEKPYACDRCSRRFARADALTRHQNSVNACSGSLSKGKRHLVDGQASRYVDGKDIDGSARSGGDYIQEPNNKSTSPVITPIHGASSVSLMNGESSSIHLSNQGTTFHHSREEEGKPQLPHYHQPPNQLPVPNNISYPSGSGHSLPVQHVAGPIPMHPSQYYNPPIYNSYTIPQMNNSGQINSAPFMNSGPHMNNVPHINNVSHMNNISHMNNLPHLNNVPHMNNVVQGDGSPMNNVILSGRPISPTMQSDHPGSPGVTFPHKHQLPIQSHVQPGTVTRVLSLPPPSQLAVIPSPTHPSQFFNASQQHQHMNGFIPSQPVHSGSQSQSQSHPGDSANLAVGESEVRDGVYIPYSRYQDLVSHAQTLQDELFRMNNRISHLEKDTKDNPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3API3</accession>
    <name>G3API3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Monocarboxylate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_139022</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375430.1">
        <property type="nucleotide sequence ID" value="XM_007375368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3API3"/>
    <dbReference type="FunCoup" id="G3API3">
        <property type="interactions" value="455"/>
    </dbReference>
    <dbReference type="GeneID" id="18870173"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139022"/>
    <dbReference type="eggNOG" id="KOG2504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_1_2_1"/>
    <dbReference type="InParanoid" id="G3API3"/>
    <dbReference type="OMA" id="LYAFWIN"/>
    <dbReference type="OrthoDB" id="2213137at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF315">
        <property type="entry name" value="TRANSPORTER MCH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="116"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="335"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="399"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="422"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="464"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="496"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="596" mass="65893" checksum="77917C9A6BCFFCDB" modified="2011-11-16" version="1">MSEDLHRITPLHLTTSRSSRASLSRMRSNQSELTRIITGIRDDLQLDEAEREQYRERGDPEYILASQLDLVRTHTRPRSTVDLESGKNNKEQGTEVIESISEDEEIDDGPPLDKGIAWLMAFCGMLASFSTWGASAGFGVFLNFYLSDNTFPGTSEYDYALVGGTVMCLANMLSPFGALLYKMLGFKLVCLIGVFLQTAAYILASFATKFWQLYLTQGVVVGVSFVLIFIPMTLVLPTWFLERKATSMGIVVSGAGLGGLVFCLSINQVIEQTGDQRWALRMVGFVTLFASLIVVIILKPRNQKRTPLKEGLSPEFIKRNFKLVFDVSVFKNKGICLLAFWFAIALTGYTLMLFSLSSFATSIGLTHQQGSVLTSVMNAAQTVGRPCMGLIADKVGRSNFTGILCLVNAILLYAFWINATSYGALIAFSVLIGLVIGVGSSMAQPLLADVLEDNLEKLPAGWSGVNFVVAPFCLVAEVIALALVTGGYRPYLHTQIFAGTCFIACFILLLFIREFLVQRLLSRRLEASESRLNEIKGVAGYLKSEQTSESLEGEDRGDDDDNEEEEVLVDRMDRYNRLLQGTATGFFLRVVYPIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3APT4</accession>
    <name>G3APT4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Nucleoside diphosphate kinase</fullName>
            <ecNumber evidence="3 11">2.7.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_61337</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="3 11"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375531.1">
        <property type="nucleotide sequence ID" value="XM_007375469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APT4"/>
    <dbReference type="FunCoup" id="G3APT4">
        <property type="interactions" value="883"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APT4"/>
    <dbReference type="GeneID" id="18873847"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61337"/>
    <dbReference type="eggNOG" id="KOG0888">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060216_6_3_1"/>
    <dbReference type="InParanoid" id="G3APT4"/>
    <dbReference type="OMA" id="NIWFKAD"/>
    <dbReference type="OrthoDB" id="2162449at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04413">
        <property type="entry name" value="NDPk_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000002">
        <property type="entry name" value="Nucleoside diphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001908">
        <property type="entry name" value="PRK00668.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11349">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="12">
        <location>
            <begin position="3"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="9">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW32255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="131" mass="14231" checksum="D07E8522CD4EA331" modified="2011-11-16" version="1" fragment="single">MSDERTFIAIKPDGVQRGLISAILGRFENRGYKLVGIKLVQPTESLLRTHYSDLQEKPFFPSLLSYMLSGPVLATVWEGKDVVKQGRAILGATNPLASAPGTIRGDFAIDMGRNVCHGSDSVESAQKEIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ03</accession>
    <name>G3AQ03_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Isocitrate dehydrogenase [NAD] subunit 1, mitochondrial</fullName>
            <ecNumber evidence="5">1.1.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Isocitric dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">NAD(+)-specific ICDH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_61400</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-threo-isocitrate + NAD(+) = 2-oxoglutarate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:23632"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.41"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Octamer of two non-identical subunits IDH1 and IDH2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.41" evidence="5"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375600.1">
        <property type="nucleotide sequence ID" value="XM_007375538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ03"/>
    <dbReference type="FunCoup" id="G3AQ03">
        <property type="interactions" value="759"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ03"/>
    <dbReference type="GeneID" id="18873875"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61400"/>
    <dbReference type="eggNOG" id="KOG0784">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_0_1_1"/>
    <dbReference type="InParanoid" id="G3AQ03"/>
    <dbReference type="OMA" id="TCAHKAN"/>
    <dbReference type="OrthoDB" id="10261637at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045242">
        <property type="term" value="C:isocitrate dehydrogenase complex (NAD+)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000001">
        <property type="entry name" value="Isocitrate dehydrogenase [NAD] subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004434">
        <property type="entry name" value="Isocitrate_DH_NAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00175">
        <property type="entry name" value="mito_nad_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF42">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="14">
        <location>
            <begin position="34"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW32324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="365" mass="39222" checksum="6FBC05ECCF92753A" modified="2011-11-16" version="1">MLRSVIAQKTSVRCLASFASPESVLPKKYGGRYTVTLIPGDGAGKEITDSVQTIFKAENVPIDWEIVEVSGVESESGHHRVDEAVESLKRNKVGLKGILYTPPGGSGKSLNVALRKELDIFASLVLIKNIPGVKSRLDGIDFALVRENTEGEYSGLEHQSYPGVVESLKIMTRFKSERIAKFAFDFAKKNNRKLVTAIHKANIMKLGDGLFRQTVKDVGQDYSGIEVNDLIVDNASMQAVAKPQQFDVLVTPNLYGSILSNIGAALIGGPGLVPGANFGREYAVFEPGCRHVGLSIKGKNSANPTAMILSAAMMLRHLGLNEQANRISKATYDVIAEGEIRTADIGGTATTTEFTDAIVAKLASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQN5</accession>
    <name>G3AQN5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_62197</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376359.1">
        <property type="nucleotide sequence ID" value="XM_007376297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQN5"/>
    <dbReference type="FunCoup" id="G3AQN5">
        <property type="interactions" value="2205"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQN5"/>
    <dbReference type="GeneID" id="18874214"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62197"/>
    <dbReference type="eggNOG" id="KOG4124">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013026_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQN5"/>
    <dbReference type="OMA" id="QPIDITY"/>
    <dbReference type="OrthoDB" id="3269380at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060963">
        <property type="term" value="P:positive regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008361">
        <property type="term" value="P:regulation of cell size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001987">
        <property type="entry name" value="Transcription factor SFP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051580">
        <property type="entry name" value="ZnF-Chromatin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23057">
        <property type="entry name" value="JUXTAPOSED WITH ANOTHER ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23057:SF0">
        <property type="entry name" value="JUXTAPOSED WITH ANOTHER ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="312"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="373"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="208"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="253"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="208"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="220"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="253"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="268"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="397" mass="44954" checksum="F0B9E7924D546714" modified="2011-11-16" version="1">MFNTKIFENSNPSGSPAINIPAASASDQMTTNNPNNIPQPIDITFNHRPSIINNNARFRRESMANSQGAGAVSWGSVTIGSWLKDEVMMSSGSNFMPYNAHHHNHMQPQQQHLINPRRGSFRAGHPSMSPHLQTSYLPDLEADYCKDYSCCGELLPTLHDLLRHYEEAHISASPPPDTNHLLNTNRNRNNFNIHNVMETVSTNDVFLNNNHHNNNNSNNTIHPTATTNNLEGSNYHFNNHQSQQAIHLQQQNPQFGQQNTQKQQRQQSPLMNSPTPNNGGTPMQTDDDEDSMYIDDPARHLYVVDNDENKPYRCPVIGCDKNYKNQNGLKYHRLHGHQGQNLRENPDGTISVIDPESNTPYLDGAALEKDKPYRCEVCGKRYKNLNGLKYHRGHTTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQN6</accession>
    <name>G3AQN6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_51580</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376361.1">
        <property type="nucleotide sequence ID" value="XM_007376299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQN6"/>
    <dbReference type="GeneID" id="18871571"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51580"/>
    <dbReference type="eggNOG" id="ENOG502SDX4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043163_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQN6"/>
    <dbReference type="OMA" id="ATERRNC"/>
    <dbReference type="OrthoDB" id="3162794at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="475" mass="54145" checksum="712DBB52C9D29A46" modified="2011-11-16" version="1">MLQDLPVELLHNIAEKLHQQEIVSLSRTNSFFNKLLLPRLYSAVTVESTPKVFENSGADSSISEIQQDRFQVGNVYLSSIKITSVYALKLFFKCLIRHPEYCSYVKVLVFKSQIPDIEDSNLFIYLKHTLYKMNNLKVFRLDKKSINVNLEWFNQGICEIQGRLVGSSPHQFNLKSLGLINTCDSSSAKTFCMKNLQELAILKSADLNVFDNLFKNSTSKIRLRKLIIENSYLDITIADLLNKHLELDQLQSLSVPLCDGSNPMKYNEFLIKLIAYMNNLSELTFTATDHRLLPSLKYLGLSKLKVLCDSNKFDLGLILAKVNSESLLRLDICPTSDISSRGKCPTSPNSSLKLLGAFSKLKMLSLPVTDEEMLVLPSYLPSSLEMLIVQCLSPDHIENSLIFCEHYGQTFQMVRENKYHARFVSIVEKFGQKLPKLKWGGFEKANKTYLYQNHEGKVMKHDGSRSDIEPIIMKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQQ2</accession>
    <name>G3AQQ2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_51593</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376377.1">
        <property type="nucleotide sequence ID" value="XM_007376315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQQ2"/>
    <dbReference type="FunCoup" id="G3AQQ2">
        <property type="interactions" value="981"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQQ2"/>
    <dbReference type="GeneID" id="18871574"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51593"/>
    <dbReference type="eggNOG" id="KOG3449">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114656_0_1_1"/>
    <dbReference type="InParanoid" id="G3AQQ2"/>
    <dbReference type="OMA" id="DIMAQGI"/>
    <dbReference type="OrthoDB" id="1227494at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002182">
        <property type="term" value="P:cytoplasmic translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05833">
        <property type="entry name" value="Ribosomal_P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001859">
        <property type="entry name" value="Ribosomal_P1/P2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044076">
        <property type="entry name" value="Ribosomal_P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00456">
        <property type="entry name" value="RIBOSOMALP2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442317" evidence="6">
        <location>
            <begin position="17"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="82"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="110" mass="10814" checksum="F923813115585AB5" modified="2011-11-16" version="1">MKYLAAYLLLVQGGNAAPAAADVAALLEAAGIESEESRISALLSELEGKNINDLIAEGNTKLASVPSGGAAVASSGAAAAGGAAAEEAAEEAAEEAKEESDDDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQR1</accession>
    <name>G3AQR1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_62218</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376386.1">
        <property type="nucleotide sequence ID" value="XM_007376324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQR1"/>
    <dbReference type="STRING" id="619300.G3AQR1"/>
    <dbReference type="GeneID" id="18874221"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62218"/>
    <dbReference type="eggNOG" id="ENOG502S4NK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036916_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQR1"/>
    <dbReference type="OMA" id="IFPVNCA"/>
    <dbReference type="OrthoDB" id="9439903at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027756">
        <property type="entry name" value="Ovo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10032:SF271">
        <property type="entry name" value="RH12261P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10032">
        <property type="entry name" value="ZINC FINGER PROTEIN WITH KRAB AND SCAN DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="351"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="226"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="228"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="276"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="290"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="509" mass="57679" checksum="564EDCBD070BB3DB" modified="2011-11-16" version="1">MIFPVGLLNNSGDVPLRASQLKTRYQGFTTTNLLTMKVGVFKFLNRLVYGTKRYLELFPPMSASTIKNINNSKFQNFFPVLCAADDLDLGFGNSDQIEFFDNMVNDHNNGKPNANTFHYDFTNANHMHGNSMNQLPMFDDFIEAHNYNHYAPKPTIPDFESGRTNPLSQTLAHPQLSYAQYGDFGIMPTRSLLPTTNDNHRRAEIMDTPYFEDFAPARRTTTQQSYLASTASISSTESMSPLSDDLFPKYDDVQESGQTSSDDESSLFNRKRAVESSPSSSEFSDISVSQKKKHTKKANKIQKPQTKKSKIVIYNEDDEPTIVTTTTTTTNNTVVRKKTYATKDGSIDNTFECPHCDATFKVKGYLTRHLKKHNSTKAFVCPFYQEPTKGNSGTKCHPTGGFSRRDTFKTHLKALHFIYPPGTKSNERNNFSGRCAGCFQHFENNHLWLETHIEANKCSGTVQCKESDPETTHGHDNEMSYYSIKSEYGQEMNSHASHYIKNEIHDILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQT5</accession>
    <name>G3AQT5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_51624</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376410.1">
        <property type="nucleotide sequence ID" value="XM_007376348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQT5"/>
    <dbReference type="FunCoup" id="G3AQT5">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQT5"/>
    <dbReference type="GeneID" id="18871577"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51624"/>
    <dbReference type="eggNOG" id="KOG0811">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059257_2_0_1"/>
    <dbReference type="InParanoid" id="G3AQT5"/>
    <dbReference type="OMA" id="LMTYTKQ"/>
    <dbReference type="OrthoDB" id="364348at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15840">
        <property type="entry name" value="SNARE_Qa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF418">
        <property type="entry name" value="SNAP RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14523">
        <property type="entry name" value="Syntaxin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="4">
        <location>
            <begin position="200"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW31632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="293" mass="33850" checksum="72EFE5DA373BBFC8" modified="2011-11-16" version="1">MSFANFDFESQRPLSKRSSTEESIDPAANELDVIIEKTSEQLQSFGHLVSQFDNQRKQIGSKRDCTQLRANIDSSVETIGEMNKAISSLISDLSQLINKSSTDKHKHHDDDEEVGSSIYVTNRQIVIKERLVTEFNELESQFRKSVRLYNEKRRVTPVREEVLQDKTDERTPLIQADRQEQQQQQLEQELIEETELQYHLLLTEERNREIEQVSEGIQEVNAIFKDLHQLVSQQGEQLSTIEDNVLQLHGNTQQAERELHKAHEYQKQKGKWSCILLVALCIVVLVIVLAVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQX6</accession>
    <name>G3AQX6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">D-lactate dehydratase</fullName>
            <ecNumber evidence="1">4.2.1.130</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_51238</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>methylglyoxal + H2O = (R)-lactate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27754"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:17158"/>
            <dbReference type="EC" id="4.2.1.130"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C56 family. HSP31-like subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.130" evidence="1"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375983.1">
        <property type="nucleotide sequence ID" value="XM_007375921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQX6"/>
    <dbReference type="FunCoup" id="G3AQX6">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQX6"/>
    <dbReference type="GeneID" id="18871537"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51238"/>
    <dbReference type="eggNOG" id="ENOG502RZ3Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070319_1_0_1"/>
    <dbReference type="InParanoid" id="G3AQX6"/>
    <dbReference type="OMA" id="LHPFEVF"/>
    <dbReference type="OrthoDB" id="543156at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019172">
        <property type="term" value="F:glyoxalase III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000051">
        <property type="entry name" value="Glutathione-independent glyoxalase HSP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050325">
        <property type="entry name" value="Prot/Nucl_acid_deglycase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48094:SF11">
        <property type="entry name" value="GLUTATHIONE-INDEPENDENT GLYOXALASE HSP31-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48094">
        <property type="entry name" value="PROTEIN/NUCLEIC ACID DEGLYCASE DJ-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="DJ-1/PfpI" evidence="6">
        <location>
            <begin position="27"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="236" mass="25462" checksum="BF1507B7E9614C9B" modified="2011-11-16" version="1">MVKALIAVTSYHGEFYPNGGKTGLFVCEAIEPFLEFRKKGYEVDFASETGTYGLDVHCTHPDYLNGEAKAAFDDPKSEYSVAIANIKKASDVNVDDYDIVFASAGHGTCFDYPTADGLHAIALSTYKRGGVVAAVCHGPLFFDNFNDPATGEPIVKGKKITGFTDEGEEYLGVFNIMKEKNLETVNQMAQKLGATYVAPNGPWDAFAVVDGKIVTGVNPQSAVVTAEKTIETYEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQZ4</accession>
    <name>G3AQZ4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prohibitin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_62270</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Prohibitin probably acts as a holdase/unfoldase for the stabilization of newly synthesized mitochondrial proteins. Involved in mitophagy; may act as an adapter for ATG8 that supports mitophagosome assembly. Negatively regulates the proteolytic processing of ATG32 via the i-AAA protease. Acts as a negative regulator of the m-AAA protease.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The mitochondrial prohibitin complex consists of two subunits (PHB1 and PHB2). The subunits assemble into a membrane-associated ring-shaped supercomplex of approximately 1 mDa. The mitochondrial prohibitin complex interacts with the m-AAA protease, a heterohexamer composed of YTA12/RCA1 and YTA10/AFG3. The mitochondrial prohibitin complex interacts with ATG8 and the interaction may support mitophagosome assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the prohibitin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376432.1">
        <property type="nucleotide sequence ID" value="XM_007376370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQZ4"/>
    <dbReference type="FunCoup" id="G3AQZ4">
        <property type="interactions" value="870"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQZ4"/>
    <dbReference type="GeneID" id="18874237"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62270"/>
    <dbReference type="eggNOG" id="KOG3083">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047969_0_2_1"/>
    <dbReference type="InParanoid" id="G3AQZ4"/>
    <dbReference type="OMA" id="YEFRLVT"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045861">
        <property type="term" value="P:negative regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03401">
        <property type="entry name" value="SPFH_prohibitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000001">
        <property type="entry name" value="Prohibitin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000163">
        <property type="entry name" value="Prohibitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222">
        <property type="entry name" value="PROHIBITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222:SF0">
        <property type="entry name" value="PROHIBITIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00679">
        <property type="entry name" value="PROHIBITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="6">
        <location>
            <begin position="26"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="273"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00057247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00063239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="282" mass="31105" checksum="A3A842945AAF7512" modified="2011-11-16" version="1">MSQRIADLISKIAVPVGLTFFLGQSAIYDVPGGKRAVIFDRLSGVKTDVIGEGTHFLIPWLQKAVIFDVRTKPKTISTTTGSKDLQNVSLTLRVLTRPEVRQLPKIYQSLGLDYDERVLPAIGNEILKSIVAQFDAAELITQREVVSARIRQELSRRANEFNIQLEDVSITHMTFGREFTKAVEQKQIAQQDAERSKYLVEKAEQEKKAAIIRAEGEAESADAVSKALAKSGDGLLMIRRLEASKQIAETLARSPNITYLPNGKGGDGEEGSKNSLLLNIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR08</accession>
    <name>G3AR08_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Glucose transporter/sensor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_155169</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376446.1">
        <property type="nucleotide sequence ID" value="XM_007376384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR08"/>
    <dbReference type="STRING" id="619300.G3AR08"/>
    <dbReference type="GeneID" id="18871014"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_155169"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_1_1"/>
    <dbReference type="InParanoid" id="G3AR08"/>
    <dbReference type="OMA" id="PRWLYKM"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010255">
        <property type="term" value="P:glucose mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000115">
        <property type="entry name" value="High-affinity glucose transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF17">
        <property type="entry name" value="HEXOSE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="27"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="200"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="286"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="349"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="382"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="416"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="453"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="29"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW31668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="521" mass="57035" checksum="09FBBFA13012DBD0" modified="2011-11-16" version="1">MGIEESLFLQKFINFGEKKAGSTTRSILVGLFSAFGGFLFGYDTGTISGILAMDFVKHQFPANRLSAEPFFTASESSLIVSILSAGTFIGALSAPLFSDRIGRKWTLILSSLIVFNLGILLQTIAEAIPLLVAGRFFAGLGVGLISAVVPLYIAETTPKWIRGAIISCYQLALTSGLLIAACVNKGTSERQDSGSYRIPIALQFAWALILGGGMICLPETPRFYISQSNEDKAKNSLRRLRKLPVDHPDLLEEYDEIKANFEFEMQFGKATWLLVLKNVNRQHHRLFVGVAIQALQQLTGINFIFYFGTQFFQSSGIKDPFLVQLATNIVNVGMTFPSIALIDILGRRSMLLAGSVVMAVSQLIVAIVGVVSDSQAANQCLVAFSCIFIAGFASTWGPLCWAVVAENFAINVRQKSVALSVASNWIWNFAIGYATPYMVDSGPGNANLGSKVFFIWGGCNVIGFIFVYFFVYETKGLTLEEVEEMYMKVDKAWNSTKFVPSGIHEASSSIEKTETEQIEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR41</accession>
    <name>G3AR41_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_67731</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376480.1">
        <property type="nucleotide sequence ID" value="XM_007376418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR41"/>
    <dbReference type="STRING" id="619300.G3AR41"/>
    <dbReference type="GeneID" id="18875179"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67731"/>
    <dbReference type="eggNOG" id="ENOG502QT0Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_410587_0_0_1"/>
    <dbReference type="InParanoid" id="G3AR41"/>
    <dbReference type="OMA" id="CYRGIRL"/>
    <dbReference type="OrthoDB" id="416217at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF2">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="18"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="669" mass="77680" checksum="5E4ACE67D072641A" modified="2011-11-16" version="1">MAKKRVGSSIKRSRTRSGCVTCRDRHIKCDEQQPVCRNCIKSKRKCYRGIRLNFTQYTLYNPNERSRRTRPRQPPNLQFRILDQSITIASLYDKGRRSYKPYLHLHTEEDLRESDLQFQQDMYSFYPSSSSVPLQDIPGPSNYPNLSLSARSGANRNTISENYQISNQLLQESYFKSGQSSGFQLPNQEFQPNQLTQQSLMYSQPVNTYGYPQSSVYFNHQLVSQPFPIQQGSQYQQYRNQFDPTRFHYGNLLYTGESSNKMDSSGYIHLLEIERYYWLLDLTNDLDLWKSIIPSLCVKAAEQDQDLFLLDCLINCSNQRDLLLEKLTQVQLEKWHNFRSIVPLCETHLPLLETLLASVCLILLSIYLRVPQEQLTGYHRSVLNNQVRLFNKVVTKAGELLRKKSGKTVVVIGCIHSVSILKYLLTRKYTLDNRLSFDSILNHDRDSFKIPRDSLEDIDEEIQYPENNPLSYPVDMSSFVNLTQFDKHNLNNSFSDFDFLQLKFASDSKKEYKSDAFKLREYFWYLIKLTYIMNNPEAAQGIEIDYNFFSKHENPASSQDGTPQPHINSFNILPNYNLQDTSPFIKPLSGSHSSTNDFPFLSDRHTVCAILTEVIAKLLNQDNQTAIQNSNCKIRNIFDQIDSSLIDSQTKLQWKCCFGWAMLGDNSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARA8</accession>
    <name>G3ARA8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Mannosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_62316</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376493.1">
        <property type="nucleotide sequence ID" value="XM_007376431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARA8"/>
    <dbReference type="FunCoup" id="G3ARA8">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARA8"/>
    <dbReference type="GeneID" id="18874268"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62316"/>
    <dbReference type="eggNOG" id="ENOG502QW2I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022381_5_0_1"/>
    <dbReference type="InParanoid" id="G3ARA8"/>
    <dbReference type="OMA" id="WNDYYAR"/>
    <dbReference type="OrthoDB" id="409543at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.20:FF:000002">
        <property type="entry name" value="Initiation-specific alpha-1,6-mannosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039367">
        <property type="entry name" value="Och1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834:SF1">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW31715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="377" mass="43043" checksum="BB703C92CF0F3DC4" modified="2011-11-16" version="1">MVQPGLKRFIFALLTLWIIYFTISSLFTSHTESVKEFSSAKLELVNALETHFDWKKTGLNFQPTSRIAITKDTIVQQQLSSAFPYDPNDSFPKKIWQTWKVGLDEHDFPHKYRRYQGTWEQKNAGYKHYVLSDAECDVLVEKLFVSVPDVAHTYKIMPKSILKADFFRYLILFAKGGVYTDIDTTALKPIDDWVSNSQVYLGVVNNAGLVVGIEADPDRPDWAEWYARRIQFCQWTIQSKKGHPMLGQLIAKITELTLQREEQGTLEKVLGKDEGGDIMNWTGPGIFTDYVFAYINSILESRASFKSRKYDGAVDWKLFTGMEQPIVLDDVMVLPITSFSPDVNTMGAGDSKHPIAYAKHMFSGSWKKDDPMPGMED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARB8</accession>
    <name>G3ARB8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Bis(5'-adenosyl)-triphosphatase</fullName>
            <ecNumber evidence="2 12">3.6.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_62330</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Cleaves A-5'-PPP-5'A to yield AMP and ADP. Can cleave all dinucleoside polyphosphates, provided the phosphate chain contains at least 3 phosphates and that 1 of the 2 bases composing the nucleotide is a purine. Is most effective on dinucleoside triphosphates. Negatively regulates intracellular dinucleoside polyphosphate levels, which elevate following heat shock.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>P(1),P(3)-bis(5'-adenosyl) triphosphate + H2O = AMP + ADP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13893"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.1.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="3.6.1.29" evidence="2 12"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376503.1">
        <property type="nucleotide sequence ID" value="XM_007376441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARB8"/>
    <dbReference type="FunCoup" id="G3ARB8">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARB8"/>
    <dbReference type="GeneID" id="18874272"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62330"/>
    <dbReference type="eggNOG" id="KOG3379">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056776_7_2_1"/>
    <dbReference type="InParanoid" id="G3ARB8"/>
    <dbReference type="OMA" id="RTIKFGP"/>
    <dbReference type="OrthoDB" id="680339at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047710">
        <property type="term" value="F:bis(5'-adenosyl)-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004081">
        <property type="term" value="F:bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015964">
        <property type="term" value="P:diadenosine triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01275">
        <property type="entry name" value="FHIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000011">
        <property type="entry name" value="Fragile histidine triad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051884">
        <property type="entry name" value="Bis(5'-adenosyl)-TPase_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039383">
        <property type="entry name" value="FHIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46243">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46243:SF1">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="13">
        <location>
            <begin position="15"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="11">
        <location>
            <begin position="108"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="8">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="103"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for induction of apoptosis" evidence="10">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW31725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="192" mass="22614" checksum="1AD87B139457EE0C" modified="2011-11-16" version="1">MTNPMEMSLRTLKSTEVYFYKYLVTSQVFYQSQHTYALVNLKPLVPGHILVVPLRTSILRFSDLTPDESIDYMNTLQLIHKFIKYIYKADALNIAIQDGPESGQSVPHLHTHVIPRYATDGYGDRIYTLLEEENLEKRYEEFKRRKEAFINSSHENKLAKKDEERMERSEEIMKNEAEWLSQELAKFIETTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARE2</accession>
    <name>G3ARE2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Uracil-DNA glycosylase</fullName>
            <shortName evidence="7">UDG</shortName>
            <ecNumber evidence="7">3.2.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">UNG1</name>
        <name evidence="10" type="ORF">SPAPADRAFT_62343</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.</text>
            <dbReference type="EC" id="3.2.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the uracil-DNA glycosylase (UDG) superfamily. UNG family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.27" evidence="7"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376527.1">
        <property type="nucleotide sequence ID" value="XM_007376465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARE2"/>
    <dbReference type="FunCoup" id="G3ARE2">
        <property type="interactions" value="305"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARE2"/>
    <dbReference type="GeneID" id="18874281"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62343"/>
    <dbReference type="eggNOG" id="KOG2994">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032162_2_2_1"/>
    <dbReference type="InParanoid" id="G3ARE2"/>
    <dbReference type="OMA" id="PDNGYLM"/>
    <dbReference type="OrthoDB" id="10031947at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097510">
        <property type="term" value="P:base-excision repair, AP site formation via deaminated base removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10027">
        <property type="entry name" value="UDG-F1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.470.10:FF:000007">
        <property type="entry name" value="Uracil-DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00148">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002043">
        <property type="entry name" value="UDG_fam1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003588">
        <property type="entry name" value="PRK05254.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003589">
        <property type="entry name" value="PRK05254.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003592">
        <property type="entry name" value="PRK05254.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00628">
        <property type="entry name" value="ung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264">
        <property type="entry name" value="URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264:SF0">
        <property type="entry name" value="URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00987">
        <property type="entry name" value="UreE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="9">
        <location>
            <begin position="163"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="358" mass="40846" checksum="B337A617089625A2" modified="2011-11-16" version="1">MSKRVLITDFFNNQGAAKNLKKKKVTAKSEPVVVKKSPSSKENTDVKKETTKEKAKVEIETEKVEPVKEEVGVEEVVEQAKPTSNYSDLCKQYNFNKLDWLKSLTPEQRELLQLEINTLHISWLAPLHKELTKPYFLKLKQFLKSQSGKTIFPPANQIYSWSHYTPLPDIKCLILGQDPYHNFNQAHGLAFSVLEPTRPPPSLVNIYKTLAIDFPQFKTPDYKAGGGGNLSQWAKRGVLLLNACLSVEAHKANSHANQGWEMFTEQVIKVAIDHHIKQNQGFVIMAWGSPAQKRVIKYNQQLSRHPDEFLVLKTVHPSPLSAHRGFFDSKVFKNCNEWLDKHNLQRIDWGLVEGNIVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARG0</accession>
    <name>G3ARG0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ADP-ribosylation factor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_141115</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376059.1">
        <property type="nucleotide sequence ID" value="XM_007375997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARG0"/>
    <dbReference type="STRING" id="619300.G3ARG0"/>
    <dbReference type="GeneID" id="18870346"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141115"/>
    <dbReference type="eggNOG" id="KOG0071">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040729_9_3_1"/>
    <dbReference type="InParanoid" id="G3ARG0"/>
    <dbReference type="OMA" id="GGQISKM"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000282">
        <property type="term" value="P:cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010513">
        <property type="term" value="P:positive regulation of phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000412">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="109"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="162" mass="18352" checksum="4E5887A44FD67F7F" modified="2011-11-16" version="1">MRILMLGLDNAGKTTVLYKLKLGKTSQTVPTVGFNVETVKHKNVSFAVWDCGGQERIRPLWRHYFTGTNALIYVVDSNDRDRLEESKKELYRVITDKELANCLLVVLANKQDYDGAIKPKDLIELFELKELSGKHTWSVIPTIATDGTGLVEMLNWISSHSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARP8</accession>
    <name>G3ARP8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatase PP2A regulatory subunit A/Splicing factor 3B subunit 1-like HEAT repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_62392</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphatase 2A regulatory subunit A family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376579.1">
        <property type="nucleotide sequence ID" value="XM_007376517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARP8"/>
    <dbReference type="FunCoup" id="G3ARP8">
        <property type="interactions" value="513"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARP8"/>
    <dbReference type="GeneID" id="18874306"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62392"/>
    <dbReference type="eggNOG" id="KOG0211">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015533_2_1_1"/>
    <dbReference type="InParanoid" id="G3ARP8"/>
    <dbReference type="OMA" id="NRVEAMQ"/>
    <dbReference type="OrthoDB" id="340346at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="C:nucleus"/>
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        <property type="term" value="C:protein phosphatase type 2A complex"/>
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        <property type="term" value="C:spindle pole body"/>
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        <property type="term" value="F:protein phosphatase regulator activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030952">
        <property type="term" value="P:establishment or maintenance of cytoskeleton polarity"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990813">
        <property type="term" value="P:meiotic centromeric cohesion protection in anaphase I"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031030">
        <property type="term" value="P:negative regulation of septation initiation signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000062">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit A alpha isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054573">
        <property type="entry name" value="PP2A/SF3B1-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051023">
        <property type="entry name" value="PP2A_Regulatory_Subunit_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648:SF4">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2 (FORMERLY 2A), REGULATORY SUBUNIT A, BETA ISOFORM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A 65 KDA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22646">
        <property type="entry name" value="PPP2R1A-like_HEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="49"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase PP2A regulatory subunit A/Splicing factor 3B subunit 1-like HEAT repeat" evidence="4">
        <location>
            <begin position="298"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="299"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="343"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase PP2A regulatory subunit A/Splicing factor 3B subunit 1-like HEAT repeat" evidence="4">
        <location>
            <begin position="376"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="382"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="421"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="546"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="598"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22646"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EGW31801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="628" mass="71674" checksum="D24850718497D8E0" modified="2011-11-16" version="1">MDNYNDDLYPLALLMDELKHDDVSNRVEAMQKLDTIAIALGPERTVKELLPFLNDVAQDDEEEVFAVLATKLGDFIPLIGGHENCEPLISILTILASMEEPLVRDKAIASLNKISLDLTNEELNGIFLNLIQNLSQGNWFSKKIASCGLFGSIILKVDSNTRKDLLKLYLKLICDDYPMVRRSAATNLPHLINLLTEFTETSPNDVNKINNQDWEIISKMFQHLINDDQDSVKFLSIDVLISILEFFNKINETSFNSDFLSSALKLIKDESWRVRYTAADRFSKIAVNFVNNESDLYQLIDPFIALMKDHEGEVRKAIAKQLPDFTRLLTKYPSTKVTIINKILPIVNELSQDQQEAVRASLASTITELSPILQKEATIEKLLPTFLIMLKDEFPDVRLNIISNLSVVNETIGINLLSTNLLPAITELAQDHKWRVRLAIIKYIPKLASQLGEPFFNDELLSLCMSWLWDPVFAIRDAAINNLKELTVIFGSDWATREILSRLLDQSDKIDEDKIDYSNFIIRLTCLFTITKLIPVLDFKVIVEKILPFINELITDNVPNIRFNVAKSYLVLVESFIEKKLELPINEQELKKLINLEILSNLEKLQNDEDVDVRFYSTKSIQGINKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3ART6</accession>
    <name>G3ART6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_61920</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007376069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ART6"/>
    <dbReference type="GeneID" id="18874111"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61920"/>
    <dbReference type="eggNOG" id="KOG1339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1180852_0_0_1"/>
    <dbReference type="InParanoid" id="G3ART6"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="3">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="235" mass="26504" checksum="0CB12BC43415F6F4" modified="2011-11-16" version="1">MDNLEATNGTVLFGAVDYSKYDYLETQPIDAHLGSQLEFGLTCNRVQFENYQTNVNKTVTKNAIPVSMSYNYPQSEFPVSILKQLVGYLGGTYNSSSSSQEVSCDFLNDTDHFISFKFGLERDKIELPVADVVEQQDDKCMLKINKSEEKYPLITFGMDILRYLYMVYDMERFEISIGIRKFNDSPANISVITKKTAKGPLDDQVNRAALSMASSGYSGATFAFILPILISLLFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARY0</accession>
    <name>G3ARY0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_72541</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376607.1">
        <property type="nucleotide sequence ID" value="XM_007376545.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="G3ARY0">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARY0"/>
    <dbReference type="GeneID" id="18875440"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72541"/>
    <dbReference type="eggNOG" id="ENOG502QVXN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028817_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARY0"/>
    <dbReference type="OMA" id="LRFKVWP"/>
    <dbReference type="OrthoDB" id="27140at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="79"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="467"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="541"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="478"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="548"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="617" mass="71684" checksum="2AD04A9F4D9C010B" modified="2011-11-16" version="1">MTESCKSSNRSSSTSSPTESPVLSAITTTEEEYDDEKKLKSEEDRKPSFNYNLEIFNLCKEYLNTKNHHGLALIARQKGIPPFLRFKVWPILLKYHPFVLNPFIQPDTDIIKEPTESGSHSASAPALSSDTNDAIKHQVRRDLAKYIQRLKYSNTKYTVSELEHEILSTLEHSIIKFSRKWGKIIKYDEALTWIALNLAEWFPPIPKTPWVLMGRDHSSPNHLLTVNLLDDYSNYIDNIPELREYLEELIYKDERISHMSFHEVYERLVLVLLHCPEPASKKKRGQELAMLKKSQSNKERKHDHSDEDNSDQPKLNKTTLPITGGTIEERVSYFIYCLRKLLPELSQFFQEEQVLTKFGTSDDEWIIWWLKFCGSKVWSRYDRGRIWDLLLGWRLQNPKKDFNYYYEKLNYPNRNTLEKLGPDIFWSLTNEEETDFKEQEDEEKTVLRQNSFRNLIEELTLSQTVVTKAAGSPDSTHKTTSSSASSVAPSLSIPFSKLDPHISLIFISLALLKSRENSLVELDQHEIRQFLSRLPSKAYKYKSKKKNGQQQGNSTSSSSSNSPVAYPIHDDRNDISSSIMISNDSAENHKVNYIDNIIQEAGELWRKWLWVEMIDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARY4</accession>
    <name>G3ARY4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_62446</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376611.1">
        <property type="nucleotide sequence ID" value="XM_007376549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARY4"/>
    <dbReference type="STRING" id="619300.G3ARY4"/>
    <dbReference type="GeneID" id="18874320"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62446"/>
    <dbReference type="eggNOG" id="KOG1577">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARY4"/>
    <dbReference type="OMA" id="METWRGM"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004032">
        <property type="term" value="F:aldose reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006066">
        <property type="term" value="P:alcohol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019568">
        <property type="term" value="P:arabinose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042843">
        <property type="term" value="P:D-xylose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042180">
        <property type="term" value="P:ketone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000018">
        <property type="entry name" value="Glycerol dehydrogenase Gcy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="8">
        <location>
            <begin position="27"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="7">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="298" mass="33344" checksum="DE51F29A4A30F333" modified="2011-11-16" version="1">MTNTPVQLKANTATITLNDGTKIPFVGLGTWQAADRDEAYNATLAALKNGYRHIDTAAIYKNEEYVGEAIKDSGIPREEIYVTTKLWNTDHKRAKEALETSLKKLGLDYVDLYLIHWPVSTDPATGENYKDWDYVDTYKALQKLYKESNGKIKSIGVSNFNKERLERLLSDPEVTIVPAVNQIEAHPFLPQPELYDYLKEKNIIIEAYSPLGSSNSPIIKNPAIVSIAEKNGVEPAQVLISWAVQRNTVVLPKSVTEARIISNLKTFTLSDEDFATLNKLAEVEGVNRICNPGWNVFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS42</accession>
    <name>G3AS42_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_51854</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376669.1">
        <property type="nucleotide sequence ID" value="XM_007376607.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AS42">
        <property type="interactions" value="121"/>
    </dbReference>
    <dbReference type="GeneID" id="18871602"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51854"/>
    <dbReference type="eggNOG" id="ENOG502SBIP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_234911_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS42"/>
    <dbReference type="OMA" id="HVIEQES"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012113165" description="Hyphally-regulated cell wall protein N-terminal domain-containing protein" evidence="7">
        <location>
            <begin position="16"/>
            <end position="1945"/>
        </location>
    </feature>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="8">
        <location>
            <begin position="7"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="460"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="647"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1033"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1194"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1790"/>
            <end position="1823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="460"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="652"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1042"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1063"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1199"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1226"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1803"/>
            <end position="1823"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1945"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1945" mass="215804" checksum="94551E4C89079EBA" modified="2011-11-16" version="1" fragment="single">MWIICLIIFIRAIFALTVTNSLLVVPPPLIYTNSEAIVVDPSGTFEFSGQESISFQLTFTNKGNFITSGNKLKKFTISDVGKIGAVIFENTGKMRIQMQNDSSANTFEMLCVTFLNTGEIIVDSTAKSGQAKSTIKSTLSWQNRGTIIIKNTSPTKATYEQACKPLFLADFIIKNSGVIKLYNQYYKQSTGNIVGNGCIYLEDGTVGELTSAYSTGNQKFVLGEDAILRVTGSKTQTYIVWNFHLGNQIGVSTAISKTNGIVYDAEIGLLTLNAGSTIMHFLIGKGYDPDLFRIEKDGKHISYQGPAPEDPYQQYYCRTYPFDDDYEGEETPKSTTYVTTYKHPNTTFTALVSVFTKTKSVWITDSKLFEHYTTTFIEDTATITGEVIVSTDDQHEWYTSTYTLSNFETSSSEFSDGDTIESEDSMEVEFSSSDLNEAAISGDISTELLIMESTENSSLDTSSYEFSYDQSSEYQNKQTLESSYSEGDQEENVETISQTSYEKVFIESTDGNNEASISDYNEFDKSLSKSSDLWSESSEGTKAWSFDESNHVRSTEVDQENFKSSLEESLQPVDYQFTSNTILLVSEEMVYSYISNDDLFAETASLSHESSKQTDYQGLEYSHNSITSEGSENEYISKETSDIFVSDQSTTDNEDEMLQEDTTEDVRSSSKSYFDISETVSIGDSSDIYAAPTEVDINGYTSEEFIGQSSAEWFPRITLETSYENEITGSTDSQEVAIGSLESNVLSSISEDTLAESFTFDQSLSSKEREDGIPNQTSAETLEITLQASSDFGVSFELTEDSTTDQSESELETESLEVIVNDSVTEKPTEYDFLTTTNSEVDTLSSLEILESSMPLSSDLMFSLRSEDSLKLSILDEFITVETTDKDSKSEDTERLVYLVSSESSLEISDEGTLDEYPTTEGILFVTDATREDSYSPKSHSISYIELSEVYDTENWETSSFVIVSSQSEQDFSQGYSDAKTQDSMKFSSMSELVESDTTSDIDYESALVEVLSSELIHDTNAETYEYGVKTISSEESTDYGESERSESSLPKDSKTIDIPGSKQDSSEIISETITKDEHTESGSNDYETLVLSNGFSRKPEETDFGEATISFTSNVKWYTEESSVTADYESKEPSGLTEGDYSSTMDNINESIPKLNTPSEVRTPFTSEILDFLSSSDFESFIIIESIESYGSDRLISGDSNDTTGGSEPSTDDVEMFESLSDNSDSSEESEEPIQDSTSVQVSHEESTNTEDYIEQSTESNSNSITFDQIVLDTTGFSESSFSLLDESTATESPSVEDSFVYTNTKESQGSHSFEITEESINSEYIHIIDSQSLESLDTTSMEEIIIEDTNLIGSTSQSDDPFAETSISEVVIYSSDRSYEEIQLSSDETIVIELTESESEENIHDSISYESIALETSIDTSNHEYFETTKQSSETIDHSPETTNSETFTLVENTKLFESTDMVSSDEFTTQNESISQLFSSETHEITATSDDVSEDVLTEQTITPALPSTSSNQEYQTENMETPQEGMSYLSTINIDYQESQSTEKEILPGESITRTEHIFFSIDSTEDTEFGLSSGQVVPIELTLSVETKGILEYTTGKYSTEETEQEYYKSEEILSIDEISTEDFKSTDDSEGLDLETSSNVVPAESINIETESLGKSIVISSEISLELIESEILTFESTSDIYKEVTSISDSLDIRISSDFNFETIPHNEPTSMSTEEDYQPISSDIISEPYAFTSLSDATILITEELPHSSSIGTGEYISHYSEEIESLAISEEVNTNETIDREHLSLPSSDEGWVDSTQESYPDNYTTESESSNIDPKIQSISSKDEYQVVTSVVDAASTELDPEQSEDKFQLDSTAEGGIEFISEESFDKEFTDDSTIPIVTPLSESIVLLESSIVTVATVESIIPESFPSELESIEYIPKSTISEEPFPELSSSDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS77</accession>
    <name>G3AS77_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_52219</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377069.1">
        <property type="nucleotide sequence ID" value="XM_007377007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS77"/>
    <dbReference type="FunCoup" id="G3AS77">
        <property type="interactions" value="153"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AS77"/>
    <dbReference type="GeneID" id="18871644"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52219"/>
    <dbReference type="eggNOG" id="KOG3548">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010407_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS77"/>
    <dbReference type="OMA" id="WPILSDV"/>
    <dbReference type="OrthoDB" id="129353at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17745">
        <property type="entry name" value="BRCT_p53bp1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047249">
        <property type="entry name" value="BRCT_p53bp1-like_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013914">
        <property type="entry name" value="Rad9_Rad53-bd_dom_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047252">
        <property type="entry name" value="TP53BP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15321:SF3">
        <property type="entry name" value="TP53-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15321">
        <property type="entry name" value="TUMOR SUPPRESSOR P53-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08605">
        <property type="entry name" value="Rad9_Rad53_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="5">
        <location>
            <begin position="650"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="5">
        <location>
            <begin position="842"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="231"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="285"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="362"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="231"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="285"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="362"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="394"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="427"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW31036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="911" mass="103042" checksum="48183F9A0655AC65" modified="2011-11-16" version="1">MKVNRMNTASNPTQQDEETHQVNISSLKIREDYNSSLVFGNDQSQIHPDITELDQTQCEIFNEDIFSTPSKKENGSIPLSYLQDTQVIEQEHIESPKSQFIGDTQVIDSPSSSDQKTRVRHVPNLPEISESPVIASYETETPSSGASHDQVRRDYQLQDTQIINNDQTQRIDDYTQRIDNDFTQQIDNYTQRIDNNFIQQIDGDTQAFTQAQTQLIDQTQKIKLESFQFSSGNQETQPINQNGNISTSSPEKQTMTSSPQLLVPPPEFISHTQVLNTQEQADITTTSQGNNDTLEVQSDDEQESENKQFEDSLLATRLKRKMSQPGSPIKRLKRNQTTMEIPEMKTPVDLRMKSEPIVLASPSTLTSQSSPPVQLVKRTSSPTKETDRENTTIPDDEEVEETTIPDEEPTRIHPPSSPQFNIPFEDGSSESEDIDDVDEPIPGKRRIRNIIDSQPTMDETTFDESNDTKVLRVETSNVLTQDDIVNHNSVWATYNNLKIYSGTVVNKDIEYSNVDFRDGIYRIKNTDLKLLDIRIGDTLNIRNSAHKYVVTGLGCSESSSFIQCIRGYNVVYLCKFPSKPKDHEFRVSLSECFMEVDDWALHESNYQLIIESHDLLSDTVPLTPTKTNKFIPFISQVSSKASPLRRSTHERTSLFSGRLFCITGIVGDRKNEIKQMIENNDGTLIDRELNEIFQYSTVGDSLVLQSDYLAGLSFGALISNSYCRSAKYLQALALGWPILSDVYIDDCINDSSTLEAWPGYLLPSGQSKRLNAVKSYDVFKFRKNYECGAMLNEQLSLNNNLLHGIDVIILTINVNANSLETSKLIFHSFGARSLQYCSKSGQIQKLFKSLGGDIIVYDDGNTINKVLSNMASRNIKKQKESKKVKVVDWEWLVQCVIGGIIWKSPTYTIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS99</accession>
    <name>G3AS99_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_62499</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376672.1">
        <property type="nucleotide sequence ID" value="XM_007376610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS99"/>
    <dbReference type="STRING" id="619300.G3AS99"/>
    <dbReference type="GeneID" id="18874350"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62499"/>
    <dbReference type="eggNOG" id="KOG1216">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029456_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS99"/>
    <dbReference type="OMA" id="CHEDKCH"/>
    <dbReference type="OrthoDB" id="4096612at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025928">
        <property type="entry name" value="Flocculin_t3_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13928">
        <property type="entry name" value="Flocculin_t3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442336" evidence="6">
        <location>
            <begin position="19"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="327" mass="33689" checksum="F3F653F8D243C4A8" modified="2011-11-16" version="1">MRFTSIIATTAFLAQAIAVNVACLVRGVQVAIVDLDTGNCPFTIPSGLPILFDFVSPQDFDIVFYYSLAAGERFFNDIVNAGDVITIPANLLFNLPGAPLYQVSVQQEPSVNSTEAVRKRLWKETVIAKRDAASDFADSLKGLDGTLVDPGAFSVVLADQPTSTPTTSPTGATTTAISTVVTVVTCTETVCKPTTVPAKPTVTTATISEVVTVFTTYCPESSITVPTFTVTLTKSDTVTTCPATPALTTITSAGKTTVVVTTVPITETVSKPTGAPTATTPGAPTVTPAPSPATATTKPAVPTFEAGAAAAGSSFLALAMIPLAYLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASC5</accession>
    <name>G3ASC5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Dihydroxyacetone kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_72612</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-glyceraldehyde + ATP = D-glyceraldehyde 3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13941"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.28"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>dihydroxyacetone + ATP = dihydroxyacetone phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15773"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16016"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.29"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Polyol metabolism; glycerol fermentation; glycerone phosphate from glycerol (oxidative route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dihydroxyacetone kinase (DAK) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376698.1">
        <property type="nucleotide sequence ID" value="XM_007376636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASC5"/>
    <dbReference type="STRING" id="619300.G3ASC5"/>
    <dbReference type="GeneID" id="18875446"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72612"/>
    <dbReference type="eggNOG" id="KOG2426">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017054_6_0_1"/>
    <dbReference type="InParanoid" id="G3ASC5"/>
    <dbReference type="OrthoDB" id="1724672at2759"/>
    <dbReference type="UniPathway" id="UPA00617">
        <property type="reaction ID" value="UER00669"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004371">
        <property type="term" value="F:glycerone kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050354">
        <property type="term" value="F:triokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019588">
        <property type="term" value="P:anaerobic glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1180.20:FF:000001">
        <property type="entry name" value="Dihydroxyacetone kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10440:FF:000001">
        <property type="entry name" value="Dihydroxyacetone kinase, DhaK subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.340:FF:000002">
        <property type="entry name" value="Dihydroxyacetone kinase, L subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10440">
        <property type="entry name" value="Dihydroxyacetone kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1180.20">
        <property type="entry name" value="Dihydroxyacetone kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004006">
        <property type="entry name" value="DhaK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004007">
        <property type="entry name" value="DhaL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036117">
        <property type="entry name" value="DhaL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050861">
        <property type="entry name" value="Dihydroxyacetone_Kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28629">
        <property type="entry name" value="TRIOKINASE/FMN CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28629:SF4">
        <property type="entry name" value="TRIOKINASE_FMN CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02733">
        <property type="entry name" value="Dak1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02734">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01120">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82549">
        <property type="entry name" value="DAK1/DegV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101473">
        <property type="entry name" value="DhaL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51481">
        <property type="entry name" value="DHAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51480">
        <property type="entry name" value="DHAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DhaK" evidence="12">
        <location>
            <begin position="10"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="DhaL" evidence="11">
        <location>
            <begin position="399"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW30665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="602" mass="66226" checksum="41BB7F34401C64A6" modified="2011-11-16" version="1">MSNKHFLPKDIGKLVPNCLKAITYEHEFLSLLEKEQVIYNNNFDRSKVALVSGGGSGHEPGFYGLVGDGMLSAVAQGNVFASPNYKNVKAAEKVCHELGAPEQEWAGCIFILTNYTGDTLYFGMAAQDLIARYGPEKIRVLRVTDDVSIKKDSNPLVGRRTLAGISIVSKVMGAASESGYSIDEVYELGKAVNDATASINAGLDHVHIPGYDMDFDFGKLGQTQLEIGLGIHNEPGVQKLNYIPTNEELVSTLLDMILNDESRGYFRHSEGDQIVLLINNLGGVSIIEEKNVLFETLEALKELYIVPTRVYTGNFVGSFNAQIFTITLFNATTAATKAFSTEEIFKLLDANTNAICWPNSKFATNKPIQNNRIIQNFIHYDDEMSTHTGTPDVFHIDPKKLEAIVRTASERIIAKEPELTEWDTKMGDGDCGMGLRKGAELILKKLNEENIAESGSILKVLHIVLNIIKDYMGGTLGAIISIFMRSVVNKVEEELNQDPSREISVIFAAALRFGLENLYSYTKAREGHRTVMDVLIPFVESFSKTMNIEKAVEVAFEAAEGTRKLRPKLGRATYIGGLDSNNDLPPDPGAYGVYEAISALAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASW6</accession>
    <name>G3ASW6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HIT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_142917</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376781.1">
        <property type="nucleotide sequence ID" value="XM_007376719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASW6"/>
    <dbReference type="STRING" id="619300.G3ASW6"/>
    <dbReference type="GeneID" id="18870501"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142917"/>
    <dbReference type="eggNOG" id="ENOG502T0EF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1224618_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASW6"/>
    <dbReference type="OMA" id="QSHYSRC"/>
    <dbReference type="OrthoDB" id="406844at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039723">
        <property type="entry name" value="Vps71/ZNHIT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007529">
        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13093">
        <property type="entry name" value="ZINC FINGER HIT DOMAIN CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51083">
        <property type="entry name" value="ZF_HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HIT-type" evidence="6">
        <location>
            <begin position="212"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="246" mass="27204" checksum="EE7955501528396A" modified="2011-11-16" version="1">MLVEEIPKSTNAHSSTIYFSSSINLTARSMNKSHVIADGGQQSGSSGTDKKGRAKVNYNVNQLMNAQIGDNRGSGTSSVGSGSGSGSGGPLKSVQQIQLERLVSKRIVELNQESSSSSKTFELPKNFTYNTIHSAGKKISQKSRLGNTPSTKRILAARRNLNSYFEEERNLISINTILQLNYQFVDFHDYENKPKSKKQKTHHIYRPKLRLCCICGSSSSYSRCSNCGLFYCSVKCNNLHQQSRCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT59</accession>
    <name>G3AT59_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62685</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376855.1">
        <property type="nucleotide sequence ID" value="XM_007376793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT59"/>
    <dbReference type="STRING" id="619300.G3AT59"/>
    <dbReference type="GeneID" id="18874434"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62685"/>
    <dbReference type="eggNOG" id="ENOG502QW5G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1180853_0_0_1"/>
    <dbReference type="InParanoid" id="G3AT59"/>
    <dbReference type="OMA" id="SHERKIC"/>
    <dbReference type="OrthoDB" id="3546279at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053157">
        <property type="entry name" value="Sterol_Uptake_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784:SF5">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="235" mass="27946" checksum="5249EF054A79FCC7" modified="2011-11-16" version="1">MNENEDKAKEMLISSFLMFAFLGVHPYGLLPLISLDKSKPDLISIARGIQTVIKQTLPVILNSELRGLMIFKDLIADSTPILEETTYPIIIQLLEDLDNTQLPSHERKICRETISTFTEALFATMYFKFPIPLFRWIIVIPDAYRDLLYEHHEFSMRLLYVFSCLCLIFQFHMFKEKNMWIDHMEEYKKYCDSRYGGFLYDLDHWLFELGVTRELRIRKYNDAGYFDPKAEYYKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATB5</accession>
    <name>G3ATB5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein kinase RIO1</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Serine/threonine-protein kinase rio1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">SPAPADRAFT_141788</name>
    </gene>
    <organism evidence="26">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 22">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376911.1">
        <property type="nucleotide sequence ID" value="XM_007376849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATB5"/>
    <dbReference type="FunCoup" id="G3ATB5">
        <property type="interactions" value="1010"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATB5"/>
    <dbReference type="GeneID" id="18870398"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141788"/>
    <dbReference type="eggNOG" id="KOG2270">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018693_4_1_1"/>
    <dbReference type="InParanoid" id="G3ATB5"/>
    <dbReference type="OMA" id="HPMSLDF"/>
    <dbReference type="OrthoDB" id="205248at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030874">
        <property type="term" value="C:nucleolar chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016479">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000234">
        <property type="term" value="P:positive regulation of rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007096">
        <property type="term" value="P:regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090234">
        <property type="term" value="P:regulation of kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05147">
        <property type="entry name" value="RIO1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000755">
        <property type="entry name" value="Homoserine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000148">
        <property type="entry name" value="Serine/threonine-protein kinase RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051272">
        <property type="entry name" value="RIO-type_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018935">
        <property type="entry name" value="RIO_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017407">
        <property type="entry name" value="Ser/Thr_kinase_Rio1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45723">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038147">
        <property type="entry name" value="Ser/Thr_PK_RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01245">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO kinase" evidence="24">
        <location>
            <begin position="63"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="373"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="380"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="419"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="23">
        <location>
            <begin position="459"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="20">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="20">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00068838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR038147-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR038147-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR038147-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="EGW30878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="481" mass="56365" checksum="FFF8240AEB0B6465" modified="2011-11-16" version="1">MTEELADKLNQVEIYSDSDSDSDLEPEVSTPSTGGTTHKEDIVTKYAHKINTDPVKSGKTHKDRANRATVEQVLDPRTMRFLAKIINKGILSRINGCISTGKEANVYHGDSDNDTREYAVKIYKTSILVFKDRERYVDGEYRFRNTKNQSNPRKMVKVWAEKEFRNLKRLYLNGIPCPEPVELRSHVLVMEYLTKGQGQPSPKLRDHPFKDIDEIVHYYHQMLYYMRRMYQECRLVHADLSEYNSIVHQDKLYIIDVSQSVEPEHPMALDFLRMDIKNVNDFFSRKKINVYPERLLFKYITEDNHSLGITDNGDEELQKFLDTLPLKTEDDNDEEVQDEIFRSLHLVRSLNNLEERDFEKFSEGKVDIMKELVANKKEESDEEETDDSEDDSEDDDDDDDDDDYDDNSDESDGEYASDDEGKPKKKWVEREAEAPKGKKYEDKDDKKARKEAAKLAKQEKRKTKMKKHIKKKIINKRKTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATJ6</accession>
    <name>G3ATJ6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62866</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376992.1">
        <property type="nucleotide sequence ID" value="XM_007376930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATJ6"/>
    <dbReference type="GeneID" id="18874498"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62866"/>
    <dbReference type="HOGENOM" id="CLU_1556232_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATJ6"/>
    <dbReference type="OrthoDB" id="10429546at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="172" mass="19526" checksum="948F8B3EAF8AC814" modified="2011-11-16" version="1">MDLIPDINDFYKSAPKLYSLAHDSSQSSVIRHAANEVLSEWGSYVVSSREYNQGLQVHHPDIHVVSKDSMRNYVFSSLLHIGVPLEKYIADAGIANLPEKLGDCFDLMSLHYADGIHYMLAHLQKQDFITMFSDATILDKYLKLRLDNASELETSSFKVAQDDPYLQHYTAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATK7</accession>
    <name>G3ATK7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_142865</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377003.1">
        <property type="nucleotide sequence ID" value="XM_007376941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATK7"/>
    <dbReference type="FunCoup" id="G3ATK7">
        <property type="interactions" value="63"/>
    </dbReference>
    <dbReference type="GeneID" id="18870495"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142865"/>
    <dbReference type="eggNOG" id="KOG2798">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030612_3_1_1"/>
    <dbReference type="InParanoid" id="G3ATK7"/>
    <dbReference type="OMA" id="SMWQGYY"/>
    <dbReference type="OrthoDB" id="978at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012901">
        <property type="entry name" value="CARME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12303:SF11">
        <property type="entry name" value="AER338CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12303">
        <property type="entry name" value="CARNOSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07942">
        <property type="entry name" value="CARME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01296">
        <property type="entry name" value="N2227"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="445" mass="50562" checksum="E2C4CD5FFF605F56" modified="2011-11-16" version="1">MPSDDLKLVFITTITCLILYYLLLTIYKNSQIPLLVSVFIFSNMVSRTEIFKNLVNTVKTATTAQPYIPDTAANGVFQTLSEFQKIELLTAMQSLKNYESASKVQVDRRKRLFKLMTWRQQKVCEQVGYLDKLSKVNSAIVANSKFINDVADHCIQKYGITYKDFDMVKEGTQGASATNYRVIEALSHYVRDWSSLDRLSDELQPIFNYINKQLSGIIPEEEKSSTCIIVPGSGLGRIAHEIGKLGYGSVHSVEYSGLMTSFVDFNYNSSTEHIYNVHPYIHQNSNFYSTESQLRSIKLSSPLCPKPYSLHLHNSDFLEFSIPEVEKYTNVVVVSVYFLDTAENLMDYLDTIQRLTTNKPIKTGYWINVGPLKYGTAAQVELNAHELKAIRQKTGWVDIDYVDSLTSPSSIGNDTGLLGYITDTESMWQGYYALNMFTSKRQENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATP7</accession>
    <name>G3ATP7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ERAD-associated E3 ubiquitin-protein ligase component HRD3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_68394</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sel-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377244.1">
        <property type="nucleotide sequence ID" value="XM_007377182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATP7"/>
    <dbReference type="FunCoup" id="G3ATP7">
        <property type="interactions" value="317"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATP7"/>
    <dbReference type="GeneID" id="18875216"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68394"/>
    <dbReference type="eggNOG" id="KOG1550">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_349489_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATP7"/>
    <dbReference type="OMA" id="DRSFSEW"/>
    <dbReference type="OrthoDB" id="27934at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050767">
        <property type="entry name" value="Sel1_AlgK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102:SF160">
        <property type="entry name" value="ERAD-ASSOCIATED E3 UBIQUITIN-PROTEIN LIGASE COMPONENT HRD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102">
        <property type="entry name" value="SEL-1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442623" description="ERAD-associated E3 ubiquitin-protein ligase component HRD3" evidence="4">
        <location>
            <begin position="23"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="740"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="769"/>
            <end position="788"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="788" mass="89884" checksum="5DD2CE3AA0B5CD48" modified="2011-11-16" version="1">MKLYNLLWWCLVYASLIHATHSGGHSYYKQAKAKLQSISKSYTRNTIYTLDNIDGNLYIPQFNNETKNYDQNISSELNPTPIELTSEVVPLLEQAISLNHTRAMVMLADLYLFGNYSMPTNYTRAKELYHQAVSLSANGHAYFMLGFIYSTGLFGEFEVNQKKANLYYQFGVENGDLNSLSVMAWRHNRGVDVPPNSTVGLEWLNEQQGQRGVNYDIRISDFNGGLYGDKMSETPSSIEVQSRVYSDLKSQLEEYSVNTNDQEFMAYYFQGLEGLKGDYFTPRNYTWAFEQFSECVQLGVDLYEHKNTHMSSVVRMFLSSCQARLAKMYLFGTGVPQNVTKAKELYDESLKVQSNAEALNDLGVIAERGLLGPANETEAVNYYVKAVKSKSHEAYRNLSLLLLKMSGGTPHSSEFRKDIFTNMKEAAYSGDIEALFHYGNYLQSGLSKYAEPDSEVTCPGTIQYYKIFIERLTQFFAPHLKYAFEELISGNFKNALVGYSIAAEQGFEPAQISAAYLLYQLQPLYSPSEAKTFNRDRVNLAIRYLERASKQGNDDATVLLGDLFSGSYTHPPNIPSPIDLDHERAFKFYRIASDRHSSHGAYKLGEMYEYGVGPSINNSIDYFMAKRYYDSSLQYKERFDLEKRATVSKSDFAGSGGYSKAHISLALLRLRIKYVFNKSAFKGNHESEGGWLNAFRKVKKNSNVASKEPVSKVDAHAEGKTYVNEQGVENYVEDYDIGDYLVISLTCMFFIIFFVQNIIRSIRRMRNNRQGNEDEDDDKRMEWQPVEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATR2</accession>
    <name>G3ATR2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Iron transport multicopper oxidase FET3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_63134</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377259.1">
        <property type="nucleotide sequence ID" value="XM_007377197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATR2"/>
    <dbReference type="STRING" id="619300.G3ATR2"/>
    <dbReference type="GeneID" id="18874611"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63134"/>
    <dbReference type="eggNOG" id="KOG1263">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006504_7_3_1"/>
    <dbReference type="InParanoid" id="G3ATR2"/>
    <dbReference type="OMA" id="CDIAPGS"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033573">
        <property type="term" value="C:high-affinity iron permease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010106">
        <property type="term" value="P:cellular response to iron ion starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033215">
        <property type="term" value="P:reductive iron assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13877">
        <property type="entry name" value="CuRO_2_Fet3p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13899">
        <property type="entry name" value="CuRO_3_Fet3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000024">
        <property type="entry name" value="FET5p Multicopper oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044130">
        <property type="entry name" value="CuRO_2_Fet3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF361">
        <property type="entry name" value="IRON TRANSPORT MULTICOPPER OXIDASE FET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0410">Iron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="9">
        <location>
            <begin position="2"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="10">
        <location>
            <begin position="164"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="400"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="415"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="47766" checksum="80E245ACA9B1D625" modified="2011-11-16" version="1">MNATWKVEPNKTYFVRLLNIGGFVSQYIWMEDHNFTVVEADGIYVEKNETSMIYITVAQRYGLLITTKNETDRNYAFMTAVDTTMLDLIPDALQLNTTNYIVYNEDADLPKPFPPPDTYLDDFYLKPLDNTTILDDADYTITLDVVMDNLGNGINYAFFNNITYVKPKVPTLMTALSAGELATNAMVYGSNTNSFVLQPNDVIDVVVNNKDTGRHPFHMHGHVFQLIERHPEVPDDQEHVAFNASDHAEWPEKPMHRDTVWLEPLSYMVLRFKATNPGVWMFHCHLEWHLDQGLAILLVEDPIGIQNDPRQQLNDNSKQICEKAGVPWEGNAAGNNTDFLVLTGENVQHAPLPAGFTARGIVALVFSCIAGILGVTCIAIYGMSDVNEEKVAQDLDVDVDMEESEDSETKYAEESCSSYAATSTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATS9</accession>
    <name>G3ATS9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CDC20/Fizzy WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_63156</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377276.1">
        <property type="nucleotide sequence ID" value="XM_007377214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATS9"/>
    <dbReference type="STRING" id="619300.G3ATS9"/>
    <dbReference type="GeneID" id="18874620"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63156"/>
    <dbReference type="eggNOG" id="KOG0305">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014831_1_1_1"/>
    <dbReference type="InParanoid" id="G3ATS9"/>
    <dbReference type="OMA" id="GKHDNRV"/>
    <dbReference type="OrthoDB" id="10263272at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF8">
        <property type="entry name" value="FI02843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="8">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="80"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW30305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="279" mass="30850" checksum="94A0233CDA5C9531" modified="2011-11-16" version="1" fragment="single">MVTSVRWSNDGSYISIGKDDGIIEIWDIETNTKLRNLNCNNHATRIAAQCWNQHILTSGDRLGNLYHSDVRISQQYVNMMSSHSAEICGIEYRNDGGQFASGGNDNLVCIWDVRNTTPLFNKSNHKAAVKALSWCPYQPSLLATGGGSSDKTINFWNTTTGSRVNTIETGSQISSLNWGYSNGTGLEIVATHGFPTNSISLFNYPTLQKTGEINSAHDSRILNGCISPDHCTLATVAGDENLKFWSLFDLVKNKREFGDVTDVDGEDKSEVETKRIKKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUC2</accession>
    <name>G3AUC2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Putative lipoate-protein ligase A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_63317</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LplA family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377469.1">
        <property type="nucleotide sequence ID" value="XM_007377407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUC2"/>
    <dbReference type="FunCoup" id="G3AUC2">
        <property type="interactions" value="268"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUC2"/>
    <dbReference type="GeneID" id="18874706"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63317"/>
    <dbReference type="eggNOG" id="KOG3159">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022986_2_1_1"/>
    <dbReference type="InParanoid" id="G3AUC2"/>
    <dbReference type="OMA" id="KCAVIGK"/>
    <dbReference type="OrthoDB" id="201621at2759"/>
    <dbReference type="UniPathway" id="UPA00537">
        <property type="reaction ID" value="UER00595"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017118">
        <property type="term" value="F:lipoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16443">
        <property type="entry name" value="LplA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004562">
        <property type="entry name" value="LipoylTrfase_LipoateP_Ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00545">
        <property type="entry name" value="lipoyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12561">
        <property type="entry name" value="LIPOATE-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12561:SF3">
        <property type="entry name" value="LIPOYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="5">
        <location>
            <begin position="95"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="394" mass="44686" checksum="CCA931E97B8EE0D3" modified="2011-11-16" version="1">MLRILRPAIARRYIHNINVPFQDDLTPAELDDFNLGDFQHYQESPSVLAHKSKLLTFEDYTVLDEPVIFNSKLTDPYMNLALEQHIYNQMPKPAANNTNRLLFYVNSPCVVVGKNQNPWKEVNLPLLNMLGIPLLRRYSGGGTVVHDLGNVNFSFMTTKEKFDRFTFAHAVSKGVNGCNGKQVQVNERGDIVTTDGFKVSGSAYKLSKGKSYHHGTMLLNSNLKVLSKLLSRSDKLGVVDAMNSVASVKSKVTNLEIESSAFIQSVSQQFEHEYGLDESVKVVEVNNDTDLPQQIQDTADELRSWQWKFGKTPKFTHELTNKSHNFTIKFHVHKSAIIEKYELTTDSDEIREKFKYLEKYISENELEYTGSNVAGYVLDDDISDWIGECIDGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUC9</accession>
    <name>G3AUC9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADPH-dependent methylglyoxal reductase GRE2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_143075</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377476.1">
        <property type="nucleotide sequence ID" value="XM_007377414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUC9"/>
    <dbReference type="FunCoup" id="G3AUC9">
        <property type="interactions" value="229"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUC9"/>
    <dbReference type="GeneID" id="18870517"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143075"/>
    <dbReference type="eggNOG" id="KOG1502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_9_2_1"/>
    <dbReference type="InParanoid" id="G3AUC9"/>
    <dbReference type="OMA" id="QGQMKEK"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05227">
        <property type="entry name" value="AR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="5"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="337" mass="36688" checksum="69314FD7C43C707D" modified="2011-11-16" version="1">MSATVFVSGATGFIAQHVVKTLLSSNYKVIGSVRSTTKGDHLKSLLNSPNFSYEIVEDVEKEGAFDQALQKHPEVTVFLHTASPFHFKATDIEKELLLPAVNGTKNALNAIHKYGSNVSRVVITSSYAAISTASKEVDPNVVVTEQSWNEISWEDAKKDPVSGYRGSKTFAEKAAWEFVKEHNPKFVVSTINPSFVFGPQAYDSEVKDSLNTSSEFINAILKNGPDATIAPTRGGFVDVRDVAKAHLVAFEKEEAKNQRLILNAGRFTLQSIIDILNENIPELKGKIPVGEPGTDKAAIAGLSTIDNSKSLAILGFELIDLKKSVVDSVQQILQARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUG2</accession>
    <name>G3AUG2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_68373</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377219.1">
        <property type="nucleotide sequence ID" value="XM_007377157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUG2"/>
    <dbReference type="GeneID" id="18875214"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68373"/>
    <dbReference type="HOGENOM" id="CLU_518918_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUG2"/>
    <dbReference type="OrthoDB" id="4022214at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012587599" description="Hyphally-regulated cell wall protein N-terminal domain-containing protein" evidence="7">
        <location>
            <begin position="16"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="8">
        <location>
            <begin position="19"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="309"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="317"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="349"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="382"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW30248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="525" mass="58585" checksum="E6EB2380DF58D328" modified="2011-11-16" version="1">MRIVGLIVFAQVIIAELLIDDTRIYTNPPNAGFEVYETCHITDTGSLQLQDMEFIKIIGDFTNYGEFLVNAYKLREHLYIEFGDTSHSFLNMGDFNIWIRGYPSSYLNVSLNSFINYGTLSLDFGSFGGVTEYYFTSNIFENEGTIDIASPGLQGKSAFGTSSITNNGIIRLSNQRYRQSPGPIIGDGCVIIGSNSLVELDSRYSCNQTFVMNNGYILFFMESHSNVYKVMNFGKWNAFIVEDGISNFVYDVNQGILTLEGFGGDYHIIIGTGYDPTLFEVMLDGHAIMYNREAPEYSVENCKDFPFDDIETESTTADDHSETSRVDESTTTEEITTSASKEVDTTSEIQDEETSEDEVSSHENVDTTTDIDDAETSEPYNDTTTHTTRTSDTVTHTEDELSVTDNQFVSTTTVVSTSYTTQICDKYGKTVDCLLMVVTNSEGVWCTSTIIIGNTDIAKTTSTSKRTNIGLYTTPDSNLKISSYTRTQTQEEIPKIYVEYSNESNLITFKITFILLATLAIVLMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUH7</accession>
    <name>G3AUH7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar import and degradation protein 27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_157038</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377234.1">
        <property type="nucleotide sequence ID" value="XM_007377172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUH7"/>
    <dbReference type="FunCoup" id="G3AUH7">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUH7"/>
    <dbReference type="GeneID" id="18871065"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157038"/>
    <dbReference type="eggNOG" id="KOG2395">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007002_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUH7"/>
    <dbReference type="OMA" id="RLNETKW"/>
    <dbReference type="OrthoDB" id="10251113at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040458">
        <property type="entry name" value="Vid27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013863">
        <property type="entry name" value="VID27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040979">
        <property type="entry name" value="Vid27_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040768">
        <property type="entry name" value="Vid27_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913:SF0">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08553">
        <property type="entry name" value="VID27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17748">
        <property type="entry name" value="VID27_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17747">
        <property type="entry name" value="VID27_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vid27 N-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Vid27 PH-like" evidence="3">
        <location>
            <begin position="232"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar import/degradation Vid27 C-terminal" evidence="2">
        <location>
            <begin position="416"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="380"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="769" mass="88162" checksum="6F658C8CB948EB8E" modified="2011-11-16" version="1">MNFLRRLIGTTTTDEIASIPSGKFYLTRSKNSPKGALECLYNDAFASIKQTTTPFYYQLFINRVCQEGEIDDVQNFADSEDDEESTSSRKDDPKDEWVFPIEENLRIYTYHKANGTRIMVWKDLNGDIGDNFEFIIDEDVRLNDIETFMFTIYKCMYENKYRTSALRIRNMDELKEFIYNPKQESLGLEGLQETYDSEEEDEEELDDEQKGEDFHYDTTPEPMGEIKAQTRNISLHLYNATTGSFVTKLEAPNVELKIIGLGAFENTLFIHSLDPSTKLRINAPLNRSMSPTFNYEHLSFIFNHYIVEGSELSGYSWLVKFPTFNDLSDFQRKFLGLMYETLNKKIWGEKSEQDYFVNAFSELTVDDAEDLTSEDRAELFVDESESEEDDEEEERKVTTKYQEDEDYFKTWQSSKDKNSDLTVGLSKGRSYVVKGDNLGVFNEGDKQLYFQTTISNLKDLHGNKFNPDKVLLHQQDQHMIMSNPKFNDKTLYKMDLTRGKIVEEWKVSNDIPVVSYAPKTKFSQLTDEQTITGISSNGLFTIDPRLPGNKLVNDQTYKSYKTKNNGFSTLATTENGYIALGSSKGDIKLFDRLGVNAKTALPSFGDAILGLDVSKDGRWLLVTCATYLLLIDAKITPGQKNEGKLGFTHYFDKEKKPIPRRLTIKPQHEAYITRENGGKALKFTRAYFNTGVNSKEMSIVTGVGSYLITWSMNKVLKNDADPYLIRKFKQNIVADNFKFGSNNEVIMATSNDVSMISKRKLDKANNIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUM5</accession>
    <name>G3AUM5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome b-c1 complex subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_68660</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the UQCRB/QCR7 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377552.1">
        <property type="nucleotide sequence ID" value="XM_007377490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUM5"/>
    <dbReference type="FunCoup" id="G3AUM5">
        <property type="interactions" value="212"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUM5"/>
    <dbReference type="GeneID" id="18875230"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68660"/>
    <dbReference type="eggNOG" id="KOG3440">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115154_1_1_1"/>
    <dbReference type="InParanoid" id="G3AUM5"/>
    <dbReference type="OMA" id="PLAQWYT"/>
    <dbReference type="OrthoDB" id="425749at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099617">
        <property type="term" value="C:matrix side of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1090.10:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1090.10">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003197">
        <property type="entry name" value="QCR7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036544">
        <property type="entry name" value="QCR7_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12022:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12022">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 14 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02271">
        <property type="entry name" value="UCR_14kD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000022">
        <property type="entry name" value="Bc1_14K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81524">
        <property type="entry name" value="14 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="124" mass="14241" checksum="B69A6AEEA3D9A359" modified="2011-11-16" version="1">MASITSIVKTCDFIRSRPVLSRVLIPLSQLFTSFSGYRQMGLKYNDLLMEETPVMQTAIRRLPEDLSYARNYRILTAHQLALTHQLLPADKAVKPEEDDHYLIPYILEAEAEAFEKAELDNIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUP6</accession>
    <name>G3AUP6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_68677</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377573.1">
        <property type="nucleotide sequence ID" value="XM_007377511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUP6"/>
    <dbReference type="GeneID" id="18875232"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68677"/>
    <dbReference type="HOGENOM" id="CLU_2098358_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUP6"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="116" mass="13019" checksum="2B4AF5262C7B2521" modified="2011-11-16" version="1">MARSNPTTPEEPFQKPSCTMSANLRRKLKHYYDNYNPDVDFFGLNDILVPIETYVVTNHPNENGGALTNQSNETIDKQEAVSSSIRFDNILSGLISFPAKSLPEDATTMENMFSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUT4</accession>
    <name>G3AUT4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Vacuolar sorting protein Vps3844 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_63643</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377791.1">
        <property type="nucleotide sequence ID" value="XM_007377729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUT4"/>
    <dbReference type="STRING" id="619300.G3AUT4"/>
    <dbReference type="GeneID" id="18874848"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63643"/>
    <dbReference type="eggNOG" id="ENOG502S64Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050724_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUT4"/>
    <dbReference type="OMA" id="NKCNSHG"/>
    <dbReference type="OrthoDB" id="5583277at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053065">
        <property type="entry name" value="Archenteron_Induction-Rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024382">
        <property type="entry name" value="Vps3844_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36853:SF1">
        <property type="entry name" value="DUF3844 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36853">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12955">
        <property type="entry name" value="Vps3844_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442653" description="Vacuolar sorting protein Vps3844 C-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar sorting protein Vps3844 C-terminal" evidence="2">
        <location>
            <begin position="288"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="390" mass="43818" checksum="484705885502CEF3" modified="2011-11-16" version="1">MKLINLVSLSLLTGAFAKIHDSVSSVYELGVSPAEDTYTIGGHDALVYFSDRFGIAEHMSLRKGNPQLVDFLNQQYELHGEVSAKPNLFISVRGVSEFDQTPSFKVSSGHINKLLFRKFPKILSKHTEGKLYQLTPEIKVVTQDGTSDLFTHFQYFNEQLMQIWNRYTQGQQQVLTYKLTNDRLFINELSQLIHLGKSEEVEVKDTFFIELSSLLSIKRKIGEDSQTFQYSQKVLFNELQALTNKFNIVIFTEPETSKYVQNIHKRDYELSSVFSKRELSGVSGNGQFSSQESCEKATDKCNSHGKCIKVSGSKWGCACESTFNKTSSKTTNWAGYDCSKRDVSVQANLFLWTSIGLLVLLVGGIQLLSSIGNDPLPGVLEAATIPRKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV29</accession>
    <name>G3AV29_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein-tyrosine-phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_63721</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377869.1">
        <property type="nucleotide sequence ID" value="XM_007377807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV29"/>
    <dbReference type="FunCoup" id="G3AV29">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV29"/>
    <dbReference type="GeneID" id="18874883"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63721"/>
    <dbReference type="eggNOG" id="KOG1716">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003560_1_0_1"/>
    <dbReference type="InParanoid" id="G3AV29"/>
    <dbReference type="OMA" id="WNTSINT"/>
    <dbReference type="OrthoDB" id="273181at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033260">
        <property type="term" value="P:nuclear DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14516">
        <property type="entry name" value="DSP_fungal_PPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053239">
        <property type="entry name" value="Dual_spec_PTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047949">
        <property type="entry name" value="PPS1_DSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47550">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE PPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47550:SF1">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE PPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="3">
        <location>
            <begin position="244"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="4">
        <location>
            <begin position="343"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="52284" checksum="7951275530BF5C06" modified="2011-11-16" version="1" fragment="single">KPVTPTPHPESLANLYNDPTNSIITSHTALSIVPRANWRLFVQCHIDAQFPSLSTLSHLLFIYSISSHDTSSEYHSLEFPPSGSIGIGDCKGENLQSIINTCKLIYLYSSSASSSALSSLIYCSDGYTECSLLVLSYLMYVEDVPLEDAILTLHLKYGRPFYIFNSDVIILRKLQPLLRKFSPKRRTTIDWGKLDTLTNQDLNEILLGPVRTVPKLGHIHNSDDESDEEDEIEIDWVKEVEGSLPSKILPYLYLGSLKHASCLPLLSKLEITHIISVGETLPWLNGYKFLHNNEITHTKLAHNMEVSEILPKHGHGRHTTVKSVMKVNNLEDDGIDELARQLPHILHFIENHYQHGKILVHCRVGVSRSATVVIAEIMKRLNVSLPYAYLFTRVRRLNIIIQPNLRFMYELFKWEEKVKLKQKENGNFLDGTVMLREIDWFIMCREIMKLNIPYLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV31</accession>
    <name>G3AV31_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peroxisomal ATPase PEX1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Peroxin-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Peroxisome biosynthesis protein PAS1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_144695</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the PEX1-PEX6 AAA ATPase complex involved in peroxisome biosynthesis. The complex acts as a protein dislocase complex that mediates the ATP-dependent extraction of the PEX5 receptor from peroxisomal membranes, an essential step for PEX5 recycling. Specifically recognizes PEX5 monoubiquitinated at 'Cys-6', and pulls it out of the peroxisome lumen through the PEX2-PEX10-PEX12 retrotranslocation channel. Extraction by the PEX1-PEX6 AAA ATPase complex is accompanied by unfolding of the TPR repeats and release of bound cargo from PEX5.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377871.1">
        <property type="nucleotide sequence ID" value="XM_007377809.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV31"/>
    <dbReference type="FunCoup" id="G3AV31">
        <property type="interactions" value="211"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV31"/>
    <dbReference type="GeneID" id="18870657"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144695"/>
    <dbReference type="eggNOG" id="KOG0735">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_1_1_1"/>
    <dbReference type="InParanoid" id="G3AV31"/>
    <dbReference type="OMA" id="LSASWCA"/>
    <dbReference type="OrthoDB" id="2187at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904949">
        <property type="term" value="C:ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140318">
        <property type="term" value="F:protein transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016562">
        <property type="term" value="P:protein import into peroxisome matrix, receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19526">
        <property type="entry name" value="RecA-like_PEX1_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000149">
        <property type="entry name" value="Nuclear valosin-containing protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015342">
        <property type="entry name" value="PEX1-N_C-lobe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF12">
        <property type="entry name" value="PEROXISOMAL ATPASE PEX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09262">
        <property type="entry name" value="PEX-1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="441"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="702"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="983"/>
            <end position="1004"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW30105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1004" mass="111010" checksum="D3F79C72AC842260" modified="2011-11-16" version="1">MDNHKVNIKYKPIKSNLVNLPSHLSNLLFTANINIQDVIVEIITKSKQAKYVGWSGMSSTSPQTLEIDPVFAQAINLSDNDSVVLNLKLDNYETANINLEPVTSSDWELVELHAQNIEDKLLSQTRCVSIGQILVVYPSSTTVAKLNVVDIGDGARTYGKISPYCEIAIAPKVRDTSVKSTKSGKSSRSTAARVEEDYSEFTSVLRRGISLPHKLYNVKNQGYQVYANFQELPSELTAEPYIAVSVVSGPTTTPTKQAPNDFDIKENKRIVARLIDCQDSPKGNIALSSKLAHALNVSGQIGNIIVLKPAVKSLPKRPTVFTIHPYTLSTPAKQKDTKTQALMDAMYPELSSSAMTNFARIPSVPGVLPYGGILKFRRNEDVNAWIRPYSLDNKKPIRLELGEELLRPASFVELEEKKEVGEAIGLDAIVEDIVDSFTISPNTGTLVHGSNGSGKTLLLNLVAEKLTNQGHFVKYVSCNGLINENFQSISKSLFKYIQLCSWHKPSLLILDDLDKIIPVEMEQIDSSKSNQTTEFLISTLAKIHNQPNSNVSILVSSNSQESINKLLGGSHLIENYHHLNPPNKQLRFDLLHHYLSSKVQVGIDLMDVVGETEGYLPNDLKILADRIYHECLFSSAQTITQDHVTKAITGFTPSNLRGVNLEKSPINWNDIGGLTEAKKILTETLEWPTKYAPIFANCPLRLRSGILLYGYPGCGKTLLASAIANECGLNFISIKGPEILNKYIGASEQSVRELFERAQSAKPCILFFDEFDSIAPKRGHDSTGVTDRVVNQMLTQMDGAEGLDGVYVLAATSRPDLIDSALLRPGRLDKSVICDLPDYECRLDILQRVCNNMELADDVNLKEIAESTDGFSGADLQGLVYNGYLKAVHVVLEQEQEEVQPVDNQTIEFFKVDAKVKPHERVSLLHQIQQFTTTNEKTVKATGKNKVVITHENFVDSLKETKPSISISEKNKLSNIYNQFVSGRDGNMPDGQPSNEIGGRVTLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVC3</accession>
    <name>G3AVC3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Endopolyphosphatase</fullName>
            <ecNumber evidence="3 12">3.6.1.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_57589</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the hydrolysis of inorganic polyphosphate (polyP) chains of many hundreds of phosphate residues into shorter lengths.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[phosphate](n+1) + n H2O = (n+1) phosphate + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22452"/>
            <dbReference type="Rhea" id="RHEA-COMP:14280"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16838"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the endopolyphosphatase PPN1 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.10" evidence="3 12"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377908.1">
        <property type="nucleotide sequence ID" value="XM_007377846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVC3"/>
    <dbReference type="FunCoup" id="G3AVC3">
        <property type="interactions" value="232"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVC3"/>
    <dbReference type="GeneID" id="18872133"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57589"/>
    <dbReference type="eggNOG" id="KOG3770">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013424_1_0_1"/>
    <dbReference type="InParanoid" id="G3AVC3"/>
    <dbReference type="OMA" id="WAERYSV"/>
    <dbReference type="OrthoDB" id="348678at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000298">
        <property type="term" value="F:endopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004309">
        <property type="term" value="F:exopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006798">
        <property type="term" value="P:polyphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00842">
        <property type="entry name" value="MPP_ASMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041805">
        <property type="entry name" value="ASMase/PPN1_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012358">
        <property type="entry name" value="EndopolyPtase_N1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340:SF55">
        <property type="entry name" value="ENDOPOLYPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027093">
        <property type="entry name" value="EndopolyPtase_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 12" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="14">
        <location>
            <begin position="58"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="340"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="611"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="341"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="620"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR027093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW30142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="662" mass="76692" checksum="BB7F5D629432F64C" modified="2011-11-16" version="1">MDEKTESIQSKKTSPYISIETIPLTDKQTKDIKKLGLTPKPQVKIVTNEKGERIIHGRFLHITDIHPDPYYKAGSSLDEACHAGKGTAGKYGDAILGCDAPMILMDDTIAWIKENLKDKIDFVVWTGDNVRHDNDRQYPRTESQIFDMNQHVSDLMYEVFKKNDSTPGLDVDLVPSLGNNDVYPHNLFSPGPTLQTRELYRIWQKFIPPEQLHTFNRGAYFFQEVIPNKLAVLSINTLYLFQSNPLVDNCDKKKEPGYKLFEWLGYTLKEMRARNMKVWLSGHVPPNEKNFDISCLRKYIVWMHEYRDIIIGGLYGHMNLDHFIPLDSVAAYKSIDNANKDKKKKKKKGKKSKSKGKGKKKSKKNSIDEFEAEFFDDSVYDSDYDSDFDDDYTAPLEETNFYRVFDENFDKDFFRVQGGVPNGKVKYMETLRETLYADIKGKRKSGYLSERYSIAHVTASVIPTFNPGLRVWEYNITNLAEDLAGVKFAPWNEFFTGLDQMLESFDKYDTEPEAEEDDSFFSIFGKKNRDKTFPLPMPEDKELGPGYVKQTFTPERYVQYYADLENINSGKKDFGYEIEYSTTDDLYKMDSLVVDEWIKYGRKLGKPIKDQNNEAEAAGKKRKSKKKKNGKEKVDYEKLGKIWNEYLKNTFVSSDYENRGYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVC6</accession>
    <name>G3AVC6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL4m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_63755</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377911.1">
        <property type="nucleotide sequence ID" value="XM_007377849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVC6"/>
    <dbReference type="FunCoup" id="G3AVC6">
        <property type="interactions" value="328"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVC6"/>
    <dbReference type="GeneID" id="18874897"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63755"/>
    <dbReference type="eggNOG" id="KOG1624">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041575_4_2_1"/>
    <dbReference type="InParanoid" id="G3AVC6"/>
    <dbReference type="OMA" id="KTFGPHP"/>
    <dbReference type="OrthoDB" id="275876at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013005">
        <property type="entry name" value="Ribosomal_uL4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746:SF6">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL4M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="75"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="252" mass="28072" checksum="AF979491F63618B2" modified="2011-11-16" version="1">MFKQIRTLATQASNAKPAFHIGQPPQYTLASLRTFPSLEPHTFIPLPAKFFAAPNVRRDQLWSAVVFEADKARMGSGNVPTKGDKPFSNRKLRPQKGTGNARLGDANSPHMDNGLKPFGIKAPHDWSTDLPTKVYAQAMQHALGSKYKEGKLYIIGEGEGVKKHESDDSSIDFKYDHESQGPEFVKRHNLQNLNLLFITEDVKSNLVAGTASLAKKADVQVKEDVEVRDILKANRIYIELSALQWLIGKYST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVK2</accession>
    <name>G3AVK2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_52802</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377717.1">
        <property type="nucleotide sequence ID" value="XM_007377655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVK2"/>
    <dbReference type="GeneID" id="18871721"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52802"/>
    <dbReference type="HOGENOM" id="CLU_630321_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVK2"/>
    <dbReference type="OrthoDB" id="10513184at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="16"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW29951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="436" mass="49661" checksum="52155EB01BF07267" modified="2011-11-16" version="1">MNAITTGQIFRGSGAVSPILQLPNEILTFIFGHLNQLDTVQVLCVSRQFYKLGVERLYNSIYINDGESKLLVSSRLHTGLYLKYAIINGFDLLKQLKSSDMKHLIKSITCEEYESNQYRTLVSWFPGTKIINEGCHLVASQNIVNPSHTPVLYLNRYNYIFFNTKFQECNFSIKQLTIDFKLPVSDILDIMPMFKGVEVLDLRNTTSANLSDFEDRGFRNIKLKGLYLHLAGGGFSNEASASRLSDLFNLSTITSFGLTNCYRGNQSSEMVWLLGKLSSLTRLYLDINVELLYDAVCQVRPHTLTSLFIQDRSDISSLEYVSMLFQIPMPIWEMIIQHGESLINLGFSSRFIYPNSNSIVDVFERVYLSDDECTSVINSELEAAEVLFNDGYFPRLNMVVINEHYFEISHGNESVTIALFDRSEGNHKLFAKTKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVU0</accession>
    <name>G3AVU0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="7">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_73417</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="7"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377750.1">
        <property type="nucleotide sequence ID" value="XM_007377688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVU0"/>
    <dbReference type="FunCoup" id="G3AVU0">
        <property type="interactions" value="334"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVU0"/>
    <dbReference type="GeneID" id="18875483"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_73417"/>
    <dbReference type="eggNOG" id="KOG0169">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002738_1_2_1"/>
    <dbReference type="InParanoid" id="G3AVU0"/>
    <dbReference type="OMA" id="HWQREMS"/>
    <dbReference type="OrthoDB" id="269822at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13360">
        <property type="entry name" value="PH_PLC_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08598">
        <property type="entry name" value="PI-PLC1c_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000039">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037755">
        <property type="entry name" value="Plc1_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF36">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE BETA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4 7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PI-PLC Y-box" evidence="9">
        <location>
            <begin position="619"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="740"/>
            <end position="870"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW29984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="893" mass="102155" checksum="0C300146C0BA522E" modified="2011-11-16" version="1">MNGPVDIIKSPSMTYLADKSDMEEESCFIKKLLHLNKSADDDISSLESNSSTARSVASSITRKLQRSPFIIHPSIATDVLDSLEDEIELHPDLSKSIGHIKIPPSFVKEGLPLLKVSHKSKKRVLFYIDPANFKFNWRVVSNSSAPSQTHRLSITGSSSRIYEFTLDDIKSVYMGVEASSYREEFNISKEFEEKWITIIYFNQKKKNLKTLHLIADTNHDFKKLSVILQSLRNLRDQLAKQFLIDFDDLDSTQKKLILDKALVQPDETGEVKQVREFLSFNDILKYTKRLNINVNRKHLESIFNEVSKQENDGGCNYEQGLNFEQFKMFVSKLKKRDDIIDIWNNICGDGEVMDFQCFAKFVTKIQGESYSEEIMRKIFNLFAIKNEYWSQESLNNFLLSKFSKPIHNIETRDYFDHPLTDYYISSSHNTYLIGRQVAGDSSVEGYIRALQRGCRSIEIDVWDGPNSDNNDLSSSLESEPIVNHGRTFTTSIKFDNVIRTIKKFAFITTPLPLILSLEINCSASNQLKVVNILKEILGNSMVLAPIDDTSTIPSPAQLKHKFLIKSKKTSAFHNLVAMEDGSYTTSTTTSFSEDGSTASTKSNSFIIRRKQSTKIIEELSALGVYIQGIKYKNFSLPESKTFNHCFSLNEKSINVMIKDEDKKIALDKHNRKYFMRVYPAKMRLKSTNFIPLSYWEHGVQMVATNWQTYDLGQQLNEALFEGVDKKGYVLKPPALRAPQLRSSKRLRLFKQPPPKRIKFSVDIISAHQLPKPKEDDIINPFITFEIIGANSIQWDSPEMESGKTRIIVENGFNPIWNAKFSGELTCSYNEFVFLKFTINSSSWGAEESEVSPIGILVARMSYMKPGYRYLPINDVFGEQLVYSSLFVKIGYSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVU1</accession>
    <name>G3AVU1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Essential nuclear protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_63610</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bystin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377752.1">
        <property type="nucleotide sequence ID" value="XM_007377690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVU1"/>
    <dbReference type="FunCoup" id="G3AVU1">
        <property type="interactions" value="880"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVU1"/>
    <dbReference type="GeneID" id="18874831"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63610"/>
    <dbReference type="eggNOG" id="KOG3871">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029727_0_1_1"/>
    <dbReference type="InParanoid" id="G3AVU1"/>
    <dbReference type="OMA" id="TKLPVIW"/>
    <dbReference type="OrthoDB" id="2192561at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007955">
        <property type="entry name" value="Bystin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12821">
        <property type="entry name" value="BYSTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12821:SF0">
        <property type="entry name" value="BYSTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05291">
        <property type="entry name" value="Bystin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW29986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="460" mass="52416" checksum="C485B4936105CF54" modified="2011-11-16" version="1">MGKISVNDTKGKQRHNPLLKDISSQGGNLRTTPRSSASQAKSNKKPQQEEEGYLDAVTSRKILQLAKEQQDELREEEEGSGRPATFGESFRKQLAESDEEEESEDEQEYSDFEEVEEIYEEEEIEVDEKDAELFNKYFQSNGAAMGQGINLADKIMAKIQEKEAQDQQQSQGGKPVDAVMLPPKVIMAYEKIGQILASYTHGKLPKLFKILPSLKNWQDVLYVTNPEKWTPHATYEATKLFVSNLSANEAQKFVETVLLEKFRSSIEDSEDHSLNYHIYRALKKSLYKPAAFFKGFLLPLVDGYCTVREATIAASVLTKVSVPVLHSSVALTQLLQRDFSPATTVFIRVLIEKKYALPYQTLDELVFYFMRFRNATQEQMQIDSAGDAPQLPVVWHKAFLAFAQRYKNDITDDQRDFLLETVRQRFHHAIGPEIRRELLAGKPRLTADQEPKDGLMVDAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE82</accession>
    <name>G3AE82_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_133116</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373028.1">
        <property type="nucleotide sequence ID" value="XM_007372966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE82"/>
    <dbReference type="GeneID" id="18869723"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133116"/>
    <dbReference type="eggNOG" id="ENOG502SW24">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_631880_0_0_1"/>
    <dbReference type="InParanoid" id="G3AE82"/>
    <dbReference type="OMA" id="SWINRNS"/>
    <dbReference type="OrthoDB" id="3980807at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035278">
        <property type="entry name" value="DUF5355"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17306">
        <property type="entry name" value="DUF5355"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="434" mass="49340" checksum="451C0BD7AB6A5248" modified="2011-11-16" version="1">MIILPNTPPLPSPLPPQSNLNPSLLTSRTALQVANLNSYNDSIRYAVDLQHYLQSLIHWVKETTNSQELNKLVDYASSREICLGIPWTNTLQSDNSKREKLNQLLKSSSKIPWSLLNEIEMVLASISLIYIRIGSDLTSETIEVEADPKSLSEYNEKWKQVVNFYKTSISYTMFANKIKQLEKEGPINGVMFTFIEKISDICIQMSILSKSCWINRCSGNPATSNNGTLSRVAIYVLNELKNAKNIVSSLGTNITLDYRLWNDYLSIIEKYVSAYAGMFLSIENYQQDKLGDAIGLIHFSLLCLQSKKNIDNSDSKKFIAKLKSKVVIKKNEHILNNLNSISTLNINRSAFNDKSGVILNDLSYLFEQLIGLNLKFTKENNNLKFDRIVSWKDISQDSKWPLGTKIPVSNIKPYEPFTVEQEDNNRYIGKGAYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEE1</accession>
    <name>G3AEE1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_58926</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373141.1">
        <property type="nucleotide sequence ID" value="XM_007373079.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEE1"/>
    <dbReference type="FunCoup" id="G3AEE1">
        <property type="interactions" value="1107"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEE1"/>
    <dbReference type="MEROPS" id="M41.004"/>
    <dbReference type="GeneID" id="18872683"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58926"/>
    <dbReference type="eggNOG" id="KOG0734">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_9_3_1"/>
    <dbReference type="InParanoid" id="G3AEE1"/>
    <dbReference type="OMA" id="MSHFEWA"/>
    <dbReference type="OrthoDB" id="1413014at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141164">
        <property type="term" value="P:mitochondrial protein quality control"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000002">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000175">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FTSH 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048438">
        <property type="entry name" value="Yme1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF97">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE YME1L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21232">
        <property type="entry name" value="Yme1-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="246"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW35729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="674" mass="73920" checksum="A443A0D9AEB58FE1" modified="2011-11-16" version="1">MLTRSLIGISRVGVYRRPITPVMARVWPMMRTIQAKRLASNSIVTLLQSQEQIANTDLTNTMAQAEFYRSLLSNNYPHIVVQRYETPGIAASAECTRLYIDALNKIGKKGKAEQVSRALNDQLGSSGVVANNATAGGTPLPHGFGSRYEPVHVIVSESLLTILSRWLKWLIPVALITYGATNAFNYLVENNTMFRNTEVPDKSVDVSQSTVRFKDVQGCDEARAELEEIVDFLKDPSKFTGLGGKLPKGVLLTGPPGTGKTLLARATAGEAGVPFFFMSGSEFDELYVGVGAKRIRELFTQAREKSPAIIFIDELDAIGGKRNPKDQAYAKQTLNQLLVELDGFSQTEGIIIIGATNFPESLDKALTRPGRFDKEVVVELPDVRGRIAILKHHMENVETADDVDPSIIARGTPGLSGAELMNLVNQAAVHASQLSAPAVDMNHFEWAKDKILMGAAKKKMVITEDSRINTAYHEAGHAIMAMYSPGATPLYKATILPRGRALGITFQLPEMDKVDMSKRECLARLDVCMGGKIAEEMINGKENVTSGCASDLANATNVARAMVTSYGMSEKIGPVQLSENWESWSPDIRNMADTEVRDFLISSENRARDVLTKKKLELKRLAEGLLEYETLTREEMEKIVKGEPINKEKTMSNTVIKSKADIKGLSIPNAARDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEG6</accession>
    <name>G3AEG6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA primase large subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_131134</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the eukaryotic-type primase large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373166.1">
        <property type="nucleotide sequence ID" value="XM_007373104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEG6"/>
    <dbReference type="FunCoup" id="G3AEG6">
        <property type="interactions" value="934"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEG6"/>
    <dbReference type="GeneID" id="18869554"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131134"/>
    <dbReference type="eggNOG" id="KOG2267">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026253_1_0_1"/>
    <dbReference type="InParanoid" id="G3AEG6"/>
    <dbReference type="OMA" id="RINYKPW"/>
    <dbReference type="OrthoDB" id="421393at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07322">
        <property type="entry name" value="PriL_PriS_Eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058560">
        <property type="entry name" value="DNA_primase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016558">
        <property type="entry name" value="DNA_primase_lsu_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007238">
        <property type="entry name" value="DNA_primase_lsu_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10537">
        <property type="entry name" value="DNA PRIMASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10537:SF3">
        <property type="entry name" value="DNA PRIMASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04104">
        <property type="entry name" value="DNA_primase_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26466">
        <property type="entry name" value="DNA_primase_lrg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009449">
        <property type="entry name" value="DNA_primase_large_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0408">Iron</keyword>
    <keyword evidence="7 9 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9" id="KW-0639">Primosome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA primase large subunit C-terminal" evidence="11">
        <location>
            <begin position="313"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR009449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR009449-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW35754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="499" mass="58184" checksum="E57CA994C377DB14" modified="2011-11-16" version="1">MFRQVKRRTAGRRNFEDDKFSSSVSPSSRLYKSRLSFYDIPPMSEITLEQFEMWAIDRLKILIEIESCQARSKSLKEIETSIKPLLLKYLPLSPSGENIEQERIKDHYSHYILRLVFCRSEDLRKKFIKNETTLFKVRYNLLQPKEQQEFIELYNDRLSWNYISANEKQEVFDQLYAACGLTIKNHLLVEGENLNNEQLRQHLLVKENFIKLPFEKCANLVGSRSIFLRNGYGYLPSSLQLNLLAIEFQENLNQILLQTFHTIPRLEEDDRILPLLNNMSRNFANIQYDSGYNQDPALASDINAQSVTTDTITQHYPLCAKHLQNTLVANSHLKYNARQQLGLFLKGIGLSVDEAVKFWSYQFTKSGVMSQEKFNKEYKYNIRHQYGLEGARINYKPWDCAIIYSKPNPGRGEAHGCPYRDFSLDSLVNNLQEMGITDQQELNNVIDDVNKRDYTIACTRVFELTHKKQLATAGAKMEGLHINHPNLYFDRSRQLAKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEQ7</accession>
    <name>G3AEQ7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_132062</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373095.1">
        <property type="nucleotide sequence ID" value="XM_007373033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEQ7"/>
    <dbReference type="FunCoup" id="G3AEQ7">
        <property type="interactions" value="417"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEQ7"/>
    <dbReference type="GeneID" id="18869632"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132062"/>
    <dbReference type="eggNOG" id="KOG2189">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005230_0_2_1"/>
    <dbReference type="InParanoid" id="G3AEQ7"/>
    <dbReference type="OMA" id="FYLWFFL"/>
    <dbReference type="OrthoDB" id="10264220at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071469">
        <property type="term" value="P:cellular response to alkaline pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006797">
        <property type="term" value="P:polyphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903778">
        <property type="term" value="P:protein localization to vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="407"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="451"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="530"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="568"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="624"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="719"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="748"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="667"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW35683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="813" mass="92666" checksum="D8C6F5F4F20985B3" modified="2011-11-16" version="1">MPEKEEAMFRSADMSLVQLYVPTDVARDIIYKIGQLNIIQFRDLNSKVNEFQRSFVKELRRLDNVERQYNLFKRELVLRNIDIASFPYDMNIPQQTDIDELLENSQLLEDRVVQLKDSVETLYDNQKYLKQFRYTIQSVDNFFASQSDDVPAAGAETALLSQYDIGRAEDHVRSASSFISGVINRDKVAALQQILWRVLRGNLYYHSEELPESIYDAKHNTYVDKNSFIIFSHGSIIHDRIKKICESLDADLYDVDTLSSRRVEQLLEVNSKLTDLSTVLVESENALSSELIAISRDLGKWWEILAREKAVYQAMNRCDYDGSRKTLIAEGWVPTDSISDLTSSIQAYDQSQSIPTIINILDTNKTPPTFARTNKFTYAFQSICDAYGVGKYQEINPGLPTIITFPFMFAIMFGDLGHGFIVALAAGTLVWNEKKLATIKRDEIFDMAYTGRYILLLMGLFSMYTGFIYNDIFSKSMNLFTSGWEWPEKFAIGETLHAKYSGSPYIFGLDPAWHGTENALLFSNSYKMKLSILMGYIHMTYSYFFSLFNYRYFNSMIDIVGNFIPGLLFMQGIFGYLSLCIVYKWTVNWFAIQKQPPGLLNMLISMFLSPGTVAEPLYSGQATVQLFLLTIALICVPWLLLVKPLYLKRQLDREAAANAQYSQLPNDDLEDVHHDADAGDDDDEDHEEHGFGDIVIHQVIHTIEFCLNCVSHTASYLRLWALSLAHAQLSTVLWSMTIGNAFGSTGIVGVFAIVFLFAMWFTLTVCILVVMEGTSAMLHSLRLHWVESMSKYFEGGGVLYEPFGFKGLLDNVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEY8</accession>
    <name>G3AEY8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_57866</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372204.1">
        <property type="nucleotide sequence ID" value="XM_007372142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEY8"/>
    <dbReference type="FunCoup" id="G3AEY8">
        <property type="interactions" value="1080"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEY8"/>
    <dbReference type="GeneID" id="18872226"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57866"/>
    <dbReference type="eggNOG" id="KOG3295">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075696_1_0_1"/>
    <dbReference type="InParanoid" id="G3AEY8"/>
    <dbReference type="OMA" id="IQKNHFR"/>
    <dbReference type="OrthoDB" id="10264538at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000003">
        <property type="entry name" value="60S ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00499">
        <property type="entry name" value="Ribosomal_eL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001380">
        <property type="entry name" value="Ribosomal_eL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018256">
        <property type="entry name" value="Ribosomal_eL13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01294">
        <property type="entry name" value="Ribosomal_L13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01104">
        <property type="entry name" value="RIBOSOMAL_L13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="202" mass="22845" checksum="9494BAF45D31F972" modified="2011-11-16" version="1">MAISKNLPLLKNHFRKHWQERVRVHFDQAGKKASRRQARLSKAAKVAPKPIDSLRPVVRAPTVKYNRKVRAGRGFTLAEIKAVGLTAKYARTIGISVDHRRQNKSQETFDANVARLQEYKSKLVIFDKKTKAAEVAGFEQVSTAATFPIAQPAIESGVRAVEVPEETAYRTLRLARNDKKYKGIREKRAREKAEAEAEKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF20</accession>
    <name>G3AF20_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DUF788-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_53226</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM208 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372236.1">
        <property type="nucleotide sequence ID" value="XM_007372174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF20"/>
    <dbReference type="FunCoup" id="G3AF20">
        <property type="interactions" value="144"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF20"/>
    <dbReference type="GeneID" id="18871770"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53226"/>
    <dbReference type="eggNOG" id="KOG3269">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094308_1_1_1"/>
    <dbReference type="InParanoid" id="G3AF20"/>
    <dbReference type="OMA" id="GRPKYDA"/>
    <dbReference type="OrthoDB" id="10012212at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006624">
        <property type="term" value="P:vacuolar protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008506">
        <property type="entry name" value="SND2/TMEM208"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13505">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13505:SF7">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05620">
        <property type="entry name" value="TMEM208_SND2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="147"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="167"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="20778" checksum="9A71D5C30043FC36" modified="2011-11-16" version="1">MAAQSSKKLAAANVKTLNQLHLISAGINLLVIIAIFFLHRPASFLPWLLLSIPSFLLQYNLEASGRPKYVKEKGYDKLVKSGQDIHQQGGLFEYFFDVIYLTWLFDILMIIFGTNKVWWGYSVIPGFACYKLYGIVSPFFKNKGQQQQETTSTAEEKSASGKSKRQQKLEARREKGPTMKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF34</accession>
    <name>G3AF34_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein induced by osmotic stress</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_59018</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373223.1">
        <property type="nucleotide sequence ID" value="XM_007373161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF34"/>
    <dbReference type="STRING" id="619300.G3AF34"/>
    <dbReference type="GeneID" id="18872721"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59018"/>
    <dbReference type="eggNOG" id="KOG1502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_9_2_1"/>
    <dbReference type="InParanoid" id="G3AF34"/>
    <dbReference type="OMA" id="AWYAMSK"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05227">
        <property type="entry name" value="AR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="4"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="334" mass="36976" checksum="A1B6B554F0467CE6" modified="2011-11-16" version="1">MSTVFVSGASGFIASHTVQQLLDQGYTVIGSVRSESKGEALVKALKGNKKFSYVIVSNIADAGAFDEVLKSHPEISAFLHIASPFRMNVTDPEKETLIPAIQGTKNVLESIQKYAPQITRVVITSSDCAARENDDRNPSITLDESVWSKATYESSKDHPVLAYLGSKPLAEKLAWDFVKTEKPKFELVTVLPSYTFGPQIDDSLISPTLNQSSQVLETMLYSNPDSKLQHHTGSFIDVRDAARAHLVALTSKEAPNHRLILSSSRFTSQSVYDILSKKYPQLNTYKGEPESDVAEIATFQKLDYSKTKQILGFEYIPLQKTVVDTVDQILRVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF44</accession>
    <name>G3AF44_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PEBP family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_59025</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373232.1">
        <property type="nucleotide sequence ID" value="XM_007373170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF44"/>
    <dbReference type="GeneID" id="18872726"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59025"/>
    <dbReference type="eggNOG" id="ENOG502SQR1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083918_3_2_1"/>
    <dbReference type="InParanoid" id="G3AF44"/>
    <dbReference type="OMA" id="CEDLDPP"/>
    <dbReference type="OrthoDB" id="4025491at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00865">
        <property type="entry name" value="PEBP_bact_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005247">
        <property type="entry name" value="YbhB_YbcL/LppC-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="22146" checksum="7B8E296D8F9A4CFB" modified="2011-11-16" version="1">MSSFLARWIGYLLYNFRGRDHGLAVRLLNLEDQNPEIELTSAVFNDNDYMPLSSAGTGVGDNLSPELKIVLPSGDSATGSLVLVCQDLDAPLPNPITHLFAYDIPTNKTEFKQGDLNETDTPEGFKFGSGAFGRVGYIGPRPIPCHGDHRYIFQVFAINEKATKEFRELDTRPDLSRAIEIMKDNVISYGKLTGLYKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF82</accession>
    <name>G3AF82_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_130664</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the RSC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372283.1">
        <property type="nucleotide sequence ID" value="XM_007372221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF82"/>
    <dbReference type="FunCoup" id="G3AF82">
        <property type="interactions" value="336"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF82"/>
    <dbReference type="GeneID" id="18869511"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_130664"/>
    <dbReference type="eggNOG" id="KOG1827">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007728_2_1_1"/>
    <dbReference type="InParanoid" id="G3AF82"/>
    <dbReference type="OMA" id="PPYWYLG"/>
    <dbReference type="OrthoDB" id="1742084at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04717">
        <property type="entry name" value="BAH_polybromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05522">
        <property type="entry name" value="Bromo_Rsc1_2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.490:FF:000016">
        <property type="entry name" value="RSC complex member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037382">
        <property type="entry name" value="Rsc/polybromo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048047">
        <property type="entry name" value="RSC1/2_bromodom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062:SF21">
        <property type="entry name" value="CHROMATIN STRUCTURE-REMODELING COMPLEX SUBUNIT RSC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062">
        <property type="entry name" value="SWI/SNF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="13">
        <location>
            <begin position="44"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="13">
        <location>
            <begin position="250"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="BAH" evidence="14">
        <location>
            <begin position="371"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="184"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="619"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="730"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="624"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW34871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="797" mass="90397" checksum="97E66DB7511776B9" modified="2011-11-16" version="1">MSDRERKKLALRIRTLFDRLYKVEDNGFLIYQTFQTLPLRTGTDYYKLVKNPLSLHAVGKKIKTQKYASAQEFIDDLALISWNARYFNEPGSAVYRHAQILKEYISNVVLPKLRNDKAIPNHSSLFYPNLGDLPDDNDDPTLAGVNFGDAGESTPRREEFVSEMSVTPQADPFISMSKPLVSTSHPKQQPFINSNIHTPKHQTPSYTRERATKQAESGIRRGRPPIIDKPFETRIKLILKGFKKLRDATNDHALTKHFERLPDIKQEIDYYERIPNPISLQEIKVKVRSRKYATVDQFINDLDLMFANAQTYYGDDPYSEEFMDYQQFKKEAQIIIQMELSKSDKEVLSLSTASSDGVLRFPMDSLEVNGYTYKIGDWVLMSNPVDAEKPTVGQIFRLWSTEEGNRYCNICWYYRPEQTCHAIDRLFFKNEVCKTGQYRDHLVDEIVGPCYVIFLTRYQKGDLPEGVIPDGAPWFICEFRYNESTHVFNRIRTWKACLPDEVRDDPEQPLIPLHENRKLIKYESPLKNLLRPDAYVGMPIPDPIGGQPNTPPVSGSVYKLAPIPDDDLGQYISSPNVTPMPEHDDVATGRRAFLFTPISQLKGGGGATTAVYATSPSVLARGSPTASQDVVPTHTTTSISAPTNFVEDKHALPGSYKSLQAQIQESQAKKLQEQQLQQMQLQQHQSLFRRANTPTPTSIAPTGGNYHTSASTYSTLLAGGTLAYGLNTGVFDLGEEVEELVNKRQKLNNEGEVESEEFVFYRAPPVLLSGRKIITNSQAELGHSAAYLAWKLRREQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFE8</accession>
    <name>G3AFE8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Trehalose-6-phosphate phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TPS2</name>
        <name evidence="4" type="ORF">SPAPADRAFT_132747</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.12" evidence="4"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372349.1">
        <property type="nucleotide sequence ID" value="XM_007372287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFE8"/>
    <dbReference type="FunCoup" id="G3AFE8">
        <property type="interactions" value="237"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFE8"/>
    <dbReference type="GeneID" id="18869689"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132747"/>
    <dbReference type="eggNOG" id="KOG1050">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002351_3_0_1"/>
    <dbReference type="InParanoid" id="G3AFE8"/>
    <dbReference type="OMA" id="VHPMPIE"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005946">
        <property type="term" value="C:alpha,alpha-trehalose-phosphate synthase complex (UDP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000036">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase subunit Tps2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000131">
        <property type="entry name" value="Trehalose-6-phosphate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1020">
        <property type="entry name" value="Trehalose-6-phosphate phosphatase related protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF123">
        <property type="entry name" value="TREHALOSE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="867"/>
            <end position="887"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="887" mass="100440" checksum="2A8E1221C39F3F78" modified="2011-11-16" version="1">MTEIEELKAYPADCSTVSPKDYANHTHAKLSGRILNVMTTLPTQIISNYDPSSGDYLWDVEAVRGNSALYSSQHFLDQHSDWESHLIAWTGEINASKVTRWQNGDNVDDALYLVDEDKQELEAKLREANGSENIHPVWLLRRDQSRWRKYAEKVLWPVFHYIQGQPSDGKEEAEAWHDYVKFNEVYLNKIKSVYKPGDVILIHDYYLLLLPQLLRMEFPDAYIGLFLHIPFPSSEYFRCLSKRTQLLDGMLGADKIGFQSGSFQRHFLSCCARLLGFEVTSDKVFAFGTTISVETLPIGVDTKKIEHDAFLPDSGIEEKVTALKELYKGKKIIVGRDRLDKVRGVVQKLEAFGMFLEMYPEWREKVVLIQVSSPGFTHSANVESKVTELINQINSQYGNLNYTPILHYQMMIPKDEYLALLRVADLALITSVRDGMNTTAFEFVICQKYNSSPLVLSEFTGTASVLNEAILINPWDTVGIAKTINEAFLLSDKEKQQLEARLYEKVTSNTVQNWISTFIRDVIDHSLVTHVTNYTPTLNRPLLLKNYLNSSRRLFLFDYDGTLTPIVKEPAAAIPSDKLNRILDVLAADPKNQIWIISGRDQAFLDKWMGDKNVGLSAEHGCFMKDLGSKEWVNLAASFDMSWQAKVEEVYKDYTEKTPGSHIERKKVALTWHYRRADPELGNYQAAKCLHQLNETIAKEYDCEVMAGKANIEVRPKFLNKGEIVKRLVLHPHGSKQDLHNEHCHSDATAADQLPDFVLCLGDDLTDEDMFRSLKTIEAEWTEQGLPKNEFGSYGVYPVAVGPASKQTVATSHLNEPSQVLETLGLLAGQVSLFESAGTVELDDRGHLANSESSERSKTAIERAASLRRKASLDKEMNKKTSTNGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFG6</accession>
    <name>G3AFG6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Probable metalloprotease ARX1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Associated with ribosomal export complex protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_133186</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Probable metalloprotease involved in proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Accompanies the pre-60S particles to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M24 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372367.1">
        <property type="nucleotide sequence ID" value="XM_007372305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFG6"/>
    <dbReference type="FunCoup" id="G3AFG6">
        <property type="interactions" value="392"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFG6"/>
    <dbReference type="GeneID" id="18869727"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133186"/>
    <dbReference type="eggNOG" id="KOG2776">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_477525_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFG6"/>
    <dbReference type="OMA" id="KPSWVHS"/>
    <dbReference type="OrthoDB" id="5876363at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047113">
        <property type="entry name" value="PA2G4/ARX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804:SF102">
        <property type="entry name" value="METALLOPROTEASE ARX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804">
        <property type="entry name" value="PROTEASE FAMILY M24 METHIONYL AMINOPEPTIDASE, AMINOPEPTIDASE P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 14" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="13">
        <location>
            <begin position="26"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="563" mass="62176" checksum="F01F6161CC1C530A" modified="2011-11-16" version="1">MQLAVRQEDADVLLKQKNILDEAILEKYRVAGQITQTGLTYIKSLINNSYHLTGSKVTIQQLCLLADSFLTKLLSRQYVNTVNEKGIAHPTTINVNELINGFAPEIDDERSFTFKEGDVVTISLGVHIDGYASQVSHTMVIYPPSPDFKPAGPLLGSNADAICAAHVAVETMVALLGSSLSPEKLPNNLKNPSNTITGQQIRGLVDAIADSFNCIVVPGSKIRRVRRFLAGQAEGVVAERDFKGVVWDESHQESRLLKQATTSTELILQDSANKPTQTSNSSAVPTDEFVVLPGEVYQIDIRMAGLQNINEVGIITTEEIDHFTGKNNKQDFNCKSSVFIRDFAVTHQLKLKTSRKLLSEIDNEFSVFPFKLDYVGKNFPINLESSDEEIKKQIEAIGQDMKSHRLGASELTNRHLIKAKPIQITKFIPLEQILLTSNPTGKHGIDAAKPVLPGMEIPLPKLGISSLKLKALLKHGKNISNVRESTTVIINNNGDEVIRLTGGEKTAKPSWVHSQYKLAGEINEIVGHLIQLTKDKRFGIKVKEINPYKMKQSNMAVESMQLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG39</accession>
    <name>G3AG39_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_58373</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372590.1">
        <property type="nucleotide sequence ID" value="XM_007372528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG39"/>
    <dbReference type="STRING" id="619300.G3AG39"/>
    <dbReference type="GeneID" id="18872421"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58373"/>
    <dbReference type="eggNOG" id="KOG0756">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_5_1_1"/>
    <dbReference type="InParanoid" id="G3AG39"/>
    <dbReference type="OMA" id="TRMQSKY"/>
    <dbReference type="OrthoDB" id="44467at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071913">
        <property type="term" value="F:citrate secondary active transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006843">
        <property type="term" value="P:mitochondrial citrate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049563">
        <property type="entry name" value="TXTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788:SF5">
        <property type="entry name" value="AFR253WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788">
        <property type="entry name" value="SUCCINATE/FUMARATE MITOCHONDRIAL TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 12" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="9"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="273"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW35178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="375" mass="42157" checksum="A7974749FAA6C733" modified="2011-11-16" version="1">MNIEQPKSDNLLTALVSGCTASAFAATLTYPFDFLKTQEQLNNPELMKKFNITNGPHTLGQMSKGCSALVLGSILKNGTRLISYNWTSKFMAIDGREGKTTSAPRTVIAGAISAFFETLWIIPFENIKITMVQNQTWMNEMTRIKEKIVDKTEKNLFKREYVSPHKYYTTEIFDQYFGTAQQSKFAAAAQKQIKNPFDTLIEHYNKHPSLTLSSTIKEIYSIKGISGFTAGTFITFTRQIAITWVWLSTYNATRQLIDPHKKSDDKGWFGHDYNALEKTGLHLVSSLAVIAVTQPLDVLKSHIQSKNGKGMYRDALSTAYKLFMQQGAKSLFRGALPRWCKLIVSGGLTASIYGYVEDIVNVAGGQKMFEEKRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGH1</accession>
    <name>G3AGH1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein SIP5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_58532</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIP5 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372722.1">
        <property type="nucleotide sequence ID" value="XM_007372660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGH1"/>
    <dbReference type="FunCoup" id="G3AGH1">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGH1"/>
    <dbReference type="GeneID" id="18872491"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58532"/>
    <dbReference type="eggNOG" id="KOG2789">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009068_2_0_1"/>
    <dbReference type="InParanoid" id="G3AGH1"/>
    <dbReference type="OMA" id="ISEPANC"/>
    <dbReference type="OrthoDB" id="21471at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd24139">
        <property type="entry name" value="SIP5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039301">
        <property type="entry name" value="Sip5/DA2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31315">
        <property type="entry name" value="PROTEIN SIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31315:SF1">
        <property type="entry name" value="PROTEIN SIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="325"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="402"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="410"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="429"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="470" mass="53125" checksum="9E284B821F32EE7A" modified="2011-11-16" version="1">MGNVPTKETRSRSNTDSSSVSDFITSGGAVSIQNTIAASSRSSQRRNTTSFNGHQRSASSELRRMKRQQEKDNALEKHYHQLIVKFNEHVDGGYLAPFGTYKSNLDYNCDVVRRLIIEGKLSPFFTPLQDFDESWTDEELYIILKQLPLHALEPGFNDEEEEDDVDDHKIHKSSNFYKRQEEKSKLKALIARVKEAQKVEEQRYLESKATSTDMSSRDLLLRLYRQPRECPICFLYYPSHLNVSKCCLQPICTECFVQIKRLDPHPPHEEQANANPNEPPLPHTLISEPASCPYCASPNFGVTYVPPKDLCTGIGGIKPSQYKQYTGNGDITDEEDEKVSRTNKKTPPAMVRRKSATPELITIDMIRPDWETKLLSARNKLARKAATASAIHASNLIISDSTESASGVTDDTRRRRGSGETGRRRGNTAGSASNSNLRSIEDRMIEEALRLSIIDEEIRKKKAEEEEAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGH4</accession>
    <name>G3AGH4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA polymerase delta subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_58535</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372725.1">
        <property type="nucleotide sequence ID" value="XM_007372663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGH4"/>
    <dbReference type="STRING" id="619300.G3AGH4"/>
    <dbReference type="GeneID" id="18872494"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58535"/>
    <dbReference type="eggNOG" id="ENOG502SEDK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1582451_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGH4"/>
    <dbReference type="OrthoDB" id="514823at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904161">
        <property type="term" value="P:DNA synthesis involved in UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.20">
        <property type="entry name" value="DNA polymerase delta, p66 (Cdc27) subunit, wHTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019038">
        <property type="entry name" value="POLD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041913">
        <property type="entry name" value="POLD3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17598">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17598:SF13">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09507">
        <property type="entry name" value="CDC27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="169" mass="18872" checksum="2F01C7984F7CD2F0" modified="2011-11-16" version="1" fragment="single">MSLPPKEVEYLANELFENSKPVTYHKFSRQVNIPMEKAKTWLFEYYEKNKDKLAATFLISGATTTGRSIRLSKDESNLDDDVKKFTSISTIHVYSVSQRKLNFSNSDLALEESRFPSHISNLEQYEKNGIIKGPKIVVSANLPAVAGKPPVPAPAAKKTTSEDKPKPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGS7</accession>
    <name>G3AGS7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58628</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372822.1">
        <property type="nucleotide sequence ID" value="XM_007372760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGS7"/>
    <dbReference type="STRING" id="619300.G3AGS7"/>
    <dbReference type="GeneID" id="18872542"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58628"/>
    <dbReference type="eggNOG" id="ENOG502RQ01">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1372025_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGS7"/>
    <dbReference type="OMA" id="MIQKFTC"/>
    <dbReference type="OrthoDB" id="4014612at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="215" mass="25081" checksum="8C8117B346998F8B" modified="2011-11-16" version="1">MIQKFTCVPRSDYDVVIVSNGTVAQISAKVSQINGQLSQNEIPTNSHITYLDSFKSLIAHLHTHTADQRFKGKVIFMFFNGLEFIQEVIGDLIDHYSRFMLMSYSSKNQKIEKLDNVYFYFDSSISEAPTEPSDPYDMFMYNNHSYEYHFNTVAIIANDIIKKMNFMIGRIRGVYIDDVNLHGERMIPLQHIHRNFPKVERIDLSSYHMMNDMIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGU4</accession>
    <name>G3AGU4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SEO1</name>
        <name evidence="8" type="ORF">SPAPADRAFT_58647</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372838.1">
        <property type="nucleotide sequence ID" value="XM_007372776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGU4"/>
    <dbReference type="FunCoup" id="G3AGU4">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGU4"/>
    <dbReference type="GeneID" id="18872551"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58647"/>
    <dbReference type="eggNOG" id="KOG2533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_4_2_1"/>
    <dbReference type="InParanoid" id="G3AGU4"/>
    <dbReference type="OMA" id="WNIFCWN"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071916">
        <property type="term" value="F:dipeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000065">
        <property type="entry name" value="Putative MFS pantothenate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF15">
        <property type="entry name" value="TRANSPORTER SEO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="404"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="433"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="458"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="491"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="524"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="573" mass="66051" checksum="A47F1423C9E8B67B" modified="2011-11-16" version="1">MTQSYAATVFSRLKWGIFPTRRIVDEDENGNFIYHKHEVDDSSESFEKSSDKNVVEIEVKDSTVLEYRDEKDRPWWKFFDEYEYRVNKETKSNRKWYHWFHEDDTPEERRVILKIDLLLTFYSLMAYWVKYLDQTNLTNAYVGGLKEAINMQGNDFVNTGVMFSVGNIIFQIPFMYILYAVPLNYVLPALDLCWSILTVCLYRVENVATLKALRFLIGAFEAPSYLAYHALFASWFKGSTAEISRRAGFYYLGQYLGVLTSGLLSGAIARTMGGVHGLQSWQWIFIIDGIISVVVGFIGFYMIPGTPEDCYSIFLSDDDIRIARRRMIKDQKDHKPRENAVKHFFNAEIWKSIFTSWHFYVLCLWNIFCWNNSNAGSGSYALWLKSLTNADGSPRFGPGQLQDYTALTPGLGLIWLIITCSFADLFKSRSGAILFSQVFNVIGNVLLAVWHIPEGAKWFAFCLQYFGWAMAPVLYSWQGDICRKDIRERQVVLVSMNILAQQSTAWISVLVWPTVEAPRFLKGFSFTASSAVALSVWTIVVLWFYKREERANARENGIVLFDSSKGDIEDVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGY6</accession>
    <name>G3AGY6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flavoprotein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_47748</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HFCD (homooligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372071.1">
        <property type="nucleotide sequence ID" value="XM_007372009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGY6"/>
    <dbReference type="STRING" id="619300.G3AGY6"/>
    <dbReference type="GeneID" id="18871141"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_47748"/>
    <dbReference type="eggNOG" id="KOG0672">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019898_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGY6"/>
    <dbReference type="OMA" id="ETYPNMS"/>
    <dbReference type="OrthoDB" id="70387at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359">
        <property type="entry name" value="HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359:SF6">
        <property type="entry name" value="PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="4">
        <location>
            <begin position="284"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="527"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="88"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="121"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="533"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="585"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="605"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="689" mass="75730" checksum="050BA8F315D28FC4" modified="2011-11-16" version="1">MSNAHSTQHPKLAVDTTTAIDQSQLSPSSTKTKGKNVLHSPIPKKPAMTDQLKSNLESTPKPLTSNHSNTKRDKLPSLVVKQDPNLKSCLSQPSESTTSVHLQQKPFPPSSSQPQEPAQPRTGSSDMQPQRRQSNPPILHHTLDADHHLENQHPQFVTTDSVSPTMHGLLNSQSRLVTSPINLEQVGGGVTPVTVVQAPSQPGSVSSSAISKSASPIQQQNNFPSRVNSLRYNDNDKKYIDFVRHSANNNSISSTSVNNVATTEEKGVSPIQHPITGPEDDKLHLLIGITGCISIHQNIVLIIEKLFELYTHDKLEIQIILTKSAEWFLSDKLTKLEQLGVSKVWFSDDGEKYFLTSPFQQAYSNAAAAGSLGKHKLTAPQLLQQYSLSYKLQRWTDVLLLAPLSANTVAKLTTGIADNLLTELLHVWPIPQVPQQQSSIPIAQPKQDTTVMSYNILSPKPIIAALAFTSAMYSHPITKKQLALLQETYPNMSILKPVEKCVDIDGNISMGGMRTWREVVDFVCKKLGPPSEPEVDEDDNVDEEEENDEKDLKENDDDDEEDEDEDEEDEDNEDSSIPEYRPEAKPSVSPVIPKSSSKSPQPTKAKLEIHDDDEKLEPIIDSRKSSATGEAISANVKEPQTRRRSNTISNKELQEHEKLASQNAILNAGIGIGIATLPAKCASANSIND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHF2</accession>
    <name>G3AHF2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_134155</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373700.1">
        <property type="nucleotide sequence ID" value="XM_007373638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHF2"/>
    <dbReference type="FunCoup" id="G3AHF2">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHF2"/>
    <dbReference type="GeneID" id="18869784"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134155"/>
    <dbReference type="eggNOG" id="KOG1802">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010015_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHF2"/>
    <dbReference type="OMA" id="HPLGKIC"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006449">
        <property type="term" value="P:regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002019">
        <property type="entry name" value="DNA helicase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001247">
        <property type="entry name" value="Erythrocyte membrane-associated antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF317">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE ECM32-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="628"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="167"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="239"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="191"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="274"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="289"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="321"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1098" mass="123881" checksum="D6A2C099DF547F08" modified="2011-11-16" version="1">MSKDTTFTCLTCSETHGKDSISKHLSTTRHKNVRINELDETIECEQCEDSNIHQLSILRYGFTDMSLLCQQCSEKEKTENNETPSATYTLSNGAFFLKLDQYLKFRDLECRNCHSDENLFVGNDKNGTQYVACKACMINYDVKFVGEDDDTFLSVLLGIKEVPKNLKRSNRGRKGKFGKGKKRGKRGRRERKPDPEAEERRAHYMATKELASSIKSGTTVKAIGSALIGKQAQKDHVSKFGNFSKGKGSPQGRKTPSRGSPAHSGRHTPVGKSPVNSGKSTPARSHTPVSEERAREEHKSRPNGKSEQVKRANDIPKPGKKTNDKPKEKQANKAKDKQADKPEQSKKSNEKVVKDKKSGDKSTTQGKEHSQTHVVMDGRGDEENKSLKSKEPPKKSKSKKGKESAKSPEPSSSRKNNIPNGNELVLPPSITKYNPASEPKLTYDSLHEYYREMSFNMFLEDQLTNISNIIESEYIMIEWYEDPDKRNTQYKLSVLSDSDIVNNFRSEVFKKLKKNPFSIGQSIFLILNDEIPWYGCISAVDEVSSKNVKRGRRTKKDVIEVVVTLYDWNNQPLPKTVHAQHLKVMPASVPVSRVLNAMDSLSNNNFIKMLLGKEPIRQIVFKNFLRYTRDTLNDSQKQAIQSIFNNAITVLQGPPGTGKTSTIFEIILQLLDSLNTYPILVVAPSNIAIDNIAEKLMPTHEKDLLRVVASEKEREYNKSHHLSSICLHHKIYEGLSTRSQTIVDEIKRGKPSVGTTGYKKFVQERFQVTKKMVAQAKVIFTTTVVAGSGQLKSISKCPVVIMDEATQSSEPTTLIPLSVPGVDKFVFVGDQKQLNCFSLVPGLSLSLFERVLLNGTYKTPHMLDTQYRMHPTISEFARNRFYGGLLKDGITAEDRKMEGIPENPVYFWNTNGKAREKSVHNWLREDRGFTYTNPGEINYVIQVVKNLIIEKGIKREDIGIITPYSGQRDLISSILVKDDIINPQSEELRIEVDIDDIKNDSKPVTIHIVSGILIASIDAFQGREKNFMVMSCVRSNVEGKIGFLRDERRLNVALTRAKYGMVIIGDENCLRSGDKLWNSYMDHLKDKNSIHSSDTFVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHL6</accession>
    <name>G3AHL6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">Peroxisomal membrane protein PEX14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 10">Peroxin-14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_59613</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the PEX13-PEX14 docking complex, a translocon channel that specifically mediates the import of peroxisomal cargo proteins bound to PEX5 receptor. The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm. Mechanistically, PEX5 receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9 10">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the peroxin-14 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373764.1">
        <property type="nucleotide sequence ID" value="XM_007373702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHL6"/>
    <dbReference type="FunCoup" id="G3AHL6">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHL6"/>
    <dbReference type="GeneID" id="18872987"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59613"/>
    <dbReference type="eggNOG" id="KOG2629">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045718_0_1_1"/>
    <dbReference type="InParanoid" id="G3AHL6"/>
    <dbReference type="OMA" id="YGAYEVT"/>
    <dbReference type="OrthoDB" id="5549158at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025655">
        <property type="entry name" value="PEX14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006785">
        <property type="entry name" value="Pex14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058:SF0">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04695">
        <property type="entry name" value="Pex14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="3 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peroxisome membrane anchor protein Pex14p N-terminal" evidence="12">
        <location>
            <begin position="2"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="41"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="262"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="282"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="353"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="410" mass="45096" checksum="58D3684719B1ED13" modified="2011-11-16" version="1">MNDELINSAVAFLKDPQVVNSPLTKKVEFLETKGLNQQEIEEALRRVSEPASETATTPTTSSSTSTAVQSVRPSPPPIDYYAAPPVPERSWKDYFIMATATVGVTYGLYQVFSRYLVPSILPPTQTAIDKDKEKIEEEFLKVDKILEQLSQDQETMKTNNEAKLKDIDTVIENVNDFLSKYNKDKLKFDDDLRLMKLEIDNLSNSIEKNMSLTKENINDELSEITQELTSLKNLIKARNGQSNGKERKIAPVSSIPSASEILKRAKAKTETPSPEVPKAKPVDTPSPVVSTPSQERVGSVTIDGVTAGGIPAWQMKDKEKEEQQEREKEQQPSKEAETVVEAPSPSSSSNGIPAWQAAAATAASAPPTAKTEEEIQEKIASIGVPSWQLNANKNSEPAKDSGIPAWQRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHQ8</accession>
    <name>G3AHQ8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phosphotransferase</fullName>
            <ecNumber evidence="14">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_59652</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:16126"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17825"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22740"/>
            <dbReference type="ChEBI" id="CHEBI:4194"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:229467"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:22741"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the hexokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="14"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373806.1">
        <property type="nucleotide sequence ID" value="XM_007373744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHQ8"/>
    <dbReference type="FunCoup" id="G3AHQ8">
        <property type="interactions" value="927"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHQ8"/>
    <dbReference type="GeneID" id="18873006"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59652"/>
    <dbReference type="eggNOG" id="KOG1369">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014393_5_2_1"/>
    <dbReference type="InParanoid" id="G3AHQ8"/>
    <dbReference type="OMA" id="ADCVQQF"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00180"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019158">
        <property type="term" value="F:mannokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000092">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.367.20:FF:000004">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF16">
        <property type="entry name" value="HEXOKINASE TYPE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="15">
        <location>
            <begin position="27"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="16">
        <location>
            <begin position="227"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052721"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00057794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW34222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="483" mass="53414" checksum="46D94B1908F6CB71" modified="2011-11-16" version="1">MVHLGPKAPAHRKGTFTDVPDVLLAELKSLETQFTVSPDKLREIVAHFINELEKGLSVAGGNIPMIPGWVMDCPTGKEKGDYLAIDLGGTNLRVVLVRLGGNRDFDTTQSKYPLPGYMRTATSEQLWDFIADCLKKFVEEVYPEGCDSELPLGFTFSYPASQNKINEGVLQRWTKGWDINDVEGHDVVPMLQKAIKKVGVPIDVVALINDTTGTLVASMYTDPETIMGLIFGTGVNGAYYEIVKDIPKLQGKLSDDIAPDSPMAINCEYGAFDNELVVLPRTKYDVQIDLESPKPGQQSFEKMNSGYYLGEILRLVLLELAEEKQLIFKGQDLTKLHQAYIMDTSFPARIEEDPFENLSEVYDVFMEVLSIETTVPERKVIRRVCELIGERSARLSVCGIAAICKKRGYTKGHCAADGSVYNKYPDFKVRAAKALRDIFQWKDDEEDPITIVAAEDGSGVGAAVISALTEKRLKQGKSVGLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHV1</accession>
    <name>G3AHV1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59686</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373849.1">
        <property type="nucleotide sequence ID" value="XM_007373787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHV1"/>
    <dbReference type="STRING" id="619300.G3AHV1"/>
    <dbReference type="GeneID" id="18873027"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59686"/>
    <dbReference type="HOGENOM" id="CLU_2984776_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHV1"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="58" mass="6410" checksum="AF5A1CF47030AB13" modified="2011-11-16" version="1" fragment="single">MSYRGRGRGGYHGGNYNNNQQQHHNNQYNNNNQQNVDAFVAANSIPVEIMGWNGASSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI88</accession>
    <name>G3AI88_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Monopolin complex subunit Csm1/Pcs1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_133618</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373241.1">
        <property type="nucleotide sequence ID" value="XM_007373179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI88"/>
    <dbReference type="FunCoup" id="G3AI88">
        <property type="interactions" value="169"/>
    </dbReference>
    <dbReference type="GeneID" id="18869760"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133618"/>
    <dbReference type="eggNOG" id="ENOG502RZY3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060768_0_0_1"/>
    <dbReference type="InParanoid" id="G3AI88"/>
    <dbReference type="OMA" id="GLWFDTS"/>
    <dbReference type="OrthoDB" id="2431049at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034506">
        <property type="term" value="C:chromosome, centromeric core domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033551">
        <property type="term" value="C:monopolin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990644">
        <property type="term" value="F:microtubule site clamp"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045144">
        <property type="term" value="P:meiotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23787">
        <property type="entry name" value="RWD_CSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040349">
        <property type="entry name" value="Csm1/Pcs1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020981">
        <property type="entry name" value="Csm1/Pcs1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038608">
        <property type="entry name" value="Csm1/Pcs1_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28006">
        <property type="entry name" value="MONOPOLIN COMPLEX SUBUNIT CSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28006:SF1">
        <property type="entry name" value="MONOPOLIN COMPLEX SUBUNIT CSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12539">
        <property type="entry name" value="Csm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Monopolin complex subunit Csm1/Pcs1 C-terminal" evidence="3">
        <location>
            <begin position="179"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="119"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="295" mass="33778" checksum="7839258FD2F58502" modified="2011-11-16" version="1">MPPKPRTKSVTKSNQKSPENSRRVSSRISERVSAVEPDDDTPSATPNKKKPASNTPSRPSSKRAKGPISIITEQDILTTTDSEDLIELINDLTNTKQDEIFTKYQTKMQSQLENDHMLIQELQLELRRKNDTIDKLLDEISQLKQSQSGEAELTFESPIRKRQPMNDITLTKELENISITLDMIELLAGVRVVNFQEDDSMLYFDVKQSSPPLFITYQLIISKEFEATAQVNYVPTFLDALENEFDHGDDEVDIVENARFLKRILPDYLCEKLLFPIDTLAQFYSKVGRALHKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIC9</accession>
    <name>G3AIC9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribosomal RNA-processing protein 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_49443</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373983.1">
        <property type="nucleotide sequence ID" value="XM_007373921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIC9"/>
    <dbReference type="FunCoup" id="G3AIC9">
        <property type="interactions" value="1077"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIC9"/>
    <dbReference type="GeneID" id="18871337"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49443"/>
    <dbReference type="eggNOG" id="KOG0299">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014017_1_0_1"/>
    <dbReference type="InParanoid" id="G3AIC9"/>
    <dbReference type="OMA" id="CSLRIWK"/>
    <dbReference type="OrthoDB" id="189968at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000644">
        <property type="entry name" value="Rrp9p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039241">
        <property type="entry name" value="Rrp9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19865">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19865:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="214"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="297"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="367"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="444"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="341"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="42"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="538" mass="60659" checksum="6EB91442019D664B" modified="2011-11-16" version="1">MAGDSFLSDPSKKRKRSSKVTSTSKRSKGGRSTPVPSSTTPEYDEEISDVSDSDEEETRGQDEEGYNEDELSSDEEFADENAADKRRRLAKQYLENLKATELNEVEGHDFDAQDLDDDILSRRLQRDVAETKGYVYKFLGEKISHQFDDHIESISTRIGSKNLTSMTSHYPYLYTVSKDAELIKWDISGRKPQRIKHVKGGSKFFKLNTANVNANHHWEQINCVAASPDGKYVVTGGSDSRLIIWSSENLSCLKVLETRSAVNSIAFRRNSDQLFAACADLRIRTYSINQFTQLEILYGHQDDITDISALARETCVSVGSRDKTVMFWKIAEESRLTFRGGDSVEKKRKKKKTKPEEETVEEQEELPFHHEGSIEVVSMNDESHFVTGSDNGNIALWSLAKKKALFTERLAHGLQPRLLPQEASAETSLEIAGQQVPEPQPYSITAIHALPYSDIFVSGSFDGSVKIWRLDQEGLRSFKLIGEVSNLKGCVVKLDVVEVPDKKQIKVFVLLSKEHKFGRWLDKIPGGRNALVNFTFDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIU1</accession>
    <name>G3AIU1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vacuolar membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_135217</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373336.1">
        <property type="nucleotide sequence ID" value="XM_007373274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIU1"/>
    <dbReference type="FunCoup" id="G3AIU1">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIU1"/>
    <dbReference type="GeneID" id="18869875"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135217"/>
    <dbReference type="eggNOG" id="KOG1944">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049109_8_0_1"/>
    <dbReference type="InParanoid" id="G3AIU1"/>
    <dbReference type="OMA" id="FMCWGFI"/>
    <dbReference type="OrthoDB" id="10267969at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF50">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YOR292C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="36871" checksum="1D4746536FA9018A" modified="2011-11-16" version="1">MPLSLEDREGATDDYANPQPPRHPFIRLINSVRCHNVPVLNFIIIGILGSFLFKLTTKYTGLYMKSSLITIIVTNLVLFGISETMAQSILCFRRDQPRMDFRFNEPGHSGMSFNSNDIALTTEDRSEYRDEEEEQGDDVASISSIDEEGLDRFINYLRQDNESIVENSTIDEGFTSALSPPTQLTYFQFNRLAGFMCWGFMMAFVQCWWYKFLQIYSKDPKFIEVLRKVLTDQLCFSPISLFCFFTYGTIVLESGNWEQTKQKLSRIYLKTLIINYSVWFPVQFFNFLIVPRNFQVPFSSSVSVLWNCFLSMRNSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIV3</accession>
    <name>G3AIV3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59125</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373348.1">
        <property type="nucleotide sequence ID" value="XM_007373286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIV3"/>
    <dbReference type="GeneID" id="18872785"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59125"/>
    <dbReference type="eggNOG" id="ENOG502S8I0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1669203_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIV3"/>
    <dbReference type="OMA" id="HTPRIKK"/>
    <dbReference type="OrthoDB" id="5408025at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="188" mass="20078" checksum="4984B682B59BA337" modified="2011-11-16" version="1">MANQYIYTNYQQYSPNLVHATAINNTSPSANITYISPPGSSNNSLSSSLTSSGTQPSSASSMLSNTLGGSSIGSSGLAASIGSGVGSSYSFHTPRLRYYLSKSFDIEDDLEFCPDIPETFHTSPTMKKFNPYTATTFSPSQDLPSSSTTPGVPSNTSPRIHTPRIKKPLEIINPQTKMRIGSPAIQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIX8</accession>
    <name>G3AIX8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="4 15">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_54076</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Endoplasmic reticulum membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4 15"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007373311.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="18871833"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54076"/>
    <dbReference type="eggNOG" id="KOG3359">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008438_2_1_1"/>
    <dbReference type="InParanoid" id="G3AIX8"/>
    <dbReference type="OMA" id="IPNTLWY"/>
    <dbReference type="OrthoDB" id="292747at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23286">
        <property type="entry name" value="beta-trefoil_MIR_PMT7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ArnT-like_N"/>
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        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
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        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF50">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PMT"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="547"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="590"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="613"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="643"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="270"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="334"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW33789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="691" mass="80217" checksum="608E85055614D194" modified="2011-11-16" version="1">MSLITLAIVIRLYKIYLPPRVVFDEVHFGTFARDYYRGEFFMDVHPPLGKLTYYWVSVLFGWNGEFTFEEIGKQFDDNVPYVAMRMVSGVAGVLTVLFTYLTVRLNCRSSVAWFTAVLLMIENSQVTQSRLILLDTPLICAQSLAIYQFKKFSQTPVAFSGTWFRSLLITGISLGIAMSIKLTGLYTMGWVGILTIFQLWTILGDLNVSTRQWIMHFFSRVVCLIIVPTTIYLAAFYIHFVALPFNGSGSGSVSSHFRSTLNDTDFTDMPVEVHYGSTVTIKHLNVERYLHSHDHNYPTGSNEQQVTLYGYDYDDNNDWMIESQNKFNERLFQKGPVKDGDTIRLYHRSTGKYLHVNDVRPPLSEHEYANEVSCDGDRNLLGDINYEFTVRIVSKKPHSKNDLPLIKLRATESIFQLIHRGTKCVVMSHEAKLPKWGMGYNEVLCVDQPTIPNTLWYIESNSNPKFNETENDFAKIEFTPFTFWNKVWEYHKIMFKLNKSMTDDHVYASEPSTWPFVLRGVAYYGNESLEHLTDENGSVIYMLGNVAIYYAGSIFILISIIRYVFYLFWYLNPFIIPKESKEHSIFYTNIRDSILGWILNYFPAFAMSRKLFLHHYFPALYFAILIIGQYVDYQITRRKYFGFALLLAVLAGGLYCYVHFVPIIYGLEWTLQECNHSKWLPTWDINCMTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIZ8</accession>
    <name>G3AIZ8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_149498</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34510.1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="619300.G3AIZ8"/>
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    <dbReference type="eggNOG" id="ENOG502S4UD">
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    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="2">
        <location>
            <begin position="31"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="98" mass="11444" checksum="6D517EBB27C01A5A" modified="2011-11-16" version="1">MSDSLYSTREDQNNQRFDQLAHTLHQFRTTVNNDIHGSIQQENSILDSLSDNFSSLMVSVKRTSGELRTVMNRNASLTRIVGMILLVFFIIWMLYKFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ73</accession>
    <name>G3AJ73_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_59190</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373415.1">
        <property type="nucleotide sequence ID" value="XM_007373353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ73"/>
    <dbReference type="GeneID" id="18872815"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59190"/>
    <dbReference type="HOGENOM" id="CLU_2222935_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ73"/>
    <dbReference type="OrthoDB" id="4085221at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="101" mass="11408" checksum="374A68EDAFC7C0C3" modified="2011-11-16" version="1">MVAVQRIRSLANFKTATRHWVPITRTAYSRAHFSSRRISSNNDAQLVSNDDAFPVIKSGVSTKKESQINNNNNSNNETEHYFEPVIYSSEEVLTASAAKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJD1</accession>
    <name>G3AJD1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="15">U6 snRNA-associated Sm-like protein LSm6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_60025</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the snRNP Sm proteins family. SmF/LSm6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374174.1">
        <property type="nucleotide sequence ID" value="XM_007374112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJD1"/>
    <dbReference type="FunCoup" id="G3AJD1">
        <property type="interactions" value="828"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJD1"/>
    <dbReference type="GeneID" id="18873184"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60025"/>
    <dbReference type="eggNOG" id="KOG1783">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_7_4_1"/>
    <dbReference type="InParanoid" id="G3AJD1"/>
    <dbReference type="OMA" id="EQTVEYV"/>
    <dbReference type="OrthoDB" id="268799at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005732">
        <property type="term" value="C:sno(s)RNA-containing ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008266">
        <property type="term" value="F:poly(U) RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01726">
        <property type="entry name" value="LSm6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000037">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016487">
        <property type="entry name" value="Lsm6/sSmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F SNRNP-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021:SF1">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006609">
        <property type="entry name" value="snRNP_SmF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10 13" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8 13" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Sm" evidence="14">
        <location>
            <begin position="8"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR006609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW34590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="77" mass="8510" checksum="D33EADCBB8EFBC52" modified="2011-11-16" version="1">MSEFEKTDPSKFLSDIIGSSVIVKLQNGINYTGNLQTIDGYMNVVLDNAKECSNGKVVRDYGDVFIRGNNVLYISEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJF1</accession>
    <name>G3AJF1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flo11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_49639</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374194.1">
        <property type="nucleotide sequence ID" value="XM_007374132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJF1"/>
    <dbReference type="STRING" id="619300.G3AJF1"/>
    <dbReference type="GeneID" id="18871358"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49639"/>
    <dbReference type="eggNOG" id="ENOG502T33R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_502641_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJF1"/>
    <dbReference type="OMA" id="LPHEYEQ"/>
    <dbReference type="OrthoDB" id="4026621at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018789">
        <property type="entry name" value="Flo11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10182">
        <property type="entry name" value="Flo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01213">
        <property type="entry name" value="Flo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51824">
        <property type="entry name" value="FLO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442550" description="Flo11 domain-containing protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="Flo11" evidence="3">
        <location>
            <begin position="28"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="486"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="248"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="388"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="542" mass="59517" checksum="C16127C12FA6CB36" modified="2011-11-16" version="1">MKIIILLTYFILAVYSHTIVTVVPTPKPTPVATCKSNSASCPVVIGARAKSLGYPPLSMAQFNSVKWIYDNVYQVEVEFMMGNSITKAALSSLYLKGLQSPGDYLGGKIELFNKTKKVDKVGSAEQHFSVTWLMLAEETGRFDSSTPFTLEYVWCQKDDNSWIDQYCKTTTKSFYAQYLIDCPVDGLTDAPNVYWDPQCNAPRPEPETTTEVPEPDPTTEDPVPTTEEPETTTEDPKFTTEDPETSTEDPVPTTEDPVPTTEDPEPTTEEPEPTTEDPEPTTEEPEPTTEDPESTTEDPESTTEDPVPTTEDPVPTTEDPVPTTEDPEPTTEDPVPTTEDPVPTTEDPVPTTEDPEPTTEDPVPTTQDPVPTTEDPVPTTEDPEPTTEDPVPTTQDPVPTTQDPVPTTEDPDPKPTSQDPESPVTKQSTENTIFTETDKGSTSEITSTVVVTTLVESYIPETSYISSSANDPTLESELPHEYEQIEEDPYNSQVPPIESESIAAQPTSPLPSEEDYLQGSAHFVSANNFALLTGLLINYFMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJG9</accession>
    <name>G3AJG9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_59247</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the REI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373456.1">
        <property type="nucleotide sequence ID" value="XM_007373394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJG9"/>
    <dbReference type="FunCoup" id="G3AJG9">
        <property type="interactions" value="590"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJG9"/>
    <dbReference type="GeneID" id="18872834"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59247"/>
    <dbReference type="eggNOG" id="KOG2785">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018787_1_1_1"/>
    <dbReference type="InParanoid" id="G3AJG9"/>
    <dbReference type="OMA" id="QRYHMKT"/>
    <dbReference type="OrthoDB" id="19329at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041661">
        <property type="entry name" value="ZN622/Rei1/Reh1_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040025">
        <property type="entry name" value="Znf622/Rei1/Reh1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13182:SF8">
        <property type="entry name" value="CYTOPLASMIC 60S SUBUNIT BIOGENESIS FACTOR ZNF622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13182">
        <property type="entry name" value="ZINC FINGER PROTEIN 622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12756">
        <property type="entry name" value="zf-C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="93"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="437"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="124"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="437"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="472" mass="54316" checksum="7A836569758C8740" modified="2011-11-16" version="1">MSFMPQGSNPETASFTCNTCGIKFVSAELQRQHMKTEWHRYNLKRRVAQLPSISSEVFAEKILNSGQYKYENGRENEDEYGFYVANRKRRSTNGRQITKKSLKSQSRRIGRGASTPVSHHEIEADNEEDEEEEEEEEIDGVDHDEVSSQFSQFSLDDHDEYHEVESVDTGSELNYTESDFTDLEGEILSSEEDSDLEIYEEEAEDIDESMEEAKAIPITHCFYCGQNNHEMENNIKHMFSKHGLYIPERSFLIDVEGLLSYLSEVVSLDHECLVCGFEGKNLESIRQHISSKGHCKIPYETKEEKLAVAEFYDFMDHDEEQRQHAKAPSKKHVGFSDDPSSSAVIVEYLPAEEDNDEELEQGGNGINDNYALVQVDRSGVELTTPTGSRIGHRTMARYYRQNIPLPREPTQGQQTQALVDRRFASGLDVYQVTKQEKEIRRAEQRAKNEKERRTKSSKANYQKHFRDEILGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJI6</accession>
    <name>G3AJI6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Antiviral helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_133625</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373473.1">
        <property type="nucleotide sequence ID" value="XM_007373411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJI6"/>
    <dbReference type="FunCoup" id="G3AJI6">
        <property type="interactions" value="937"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJI6"/>
    <dbReference type="GeneID" id="18869761"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133625"/>
    <dbReference type="eggNOG" id="KOG0947">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002902_1_4_1"/>
    <dbReference type="InParanoid" id="G3AJI6"/>
    <dbReference type="OMA" id="DHVNIIM"/>
    <dbReference type="OrthoDB" id="64767at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055087">
        <property type="term" value="C:Ski complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070478">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000354">
        <property type="entry name" value="ATP-dependent RNA helicase SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000987">
        <property type="entry name" value="DEAD/DEAH box RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.30:FF:000001">
        <property type="entry name" value="Ski2 ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048392">
        <property type="entry name" value="MTR4-like_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016438">
        <property type="entry name" value="SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012961">
        <property type="entry name" value="Ski2/MTR4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048727">
        <property type="entry name" value="Ski2_beta-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040801">
        <property type="entry name" value="Ski2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE SUPV3L1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08148">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21408">
        <property type="entry name" value="MTR4-like_stalk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21409">
        <property type="entry name" value="Ski2_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17911">
        <property type="entry name" value="Ski2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005198">
        <property type="entry name" value="Antiviral_helicase_SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01142">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="259"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="555"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="75"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="454"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="454"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="475"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW33889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1211" mass="136458" checksum="EA191E57CEDC9679" modified="2011-11-16" version="1">MATSELIHSLIEPSQELTWDLLDLTTPKPTASSVAQLSNFLINPPPINRTRFRFKRTGIEGNITRYAEEICDKTESETQSLSMGRTHQGKSQTMSSRKVMPFQPGGLIKSVAKDHDDDESRLHRNQVGLFDMPPGFSRGLSVDASDSGLELEQVDDEEEESITTISMNKIDTKASTDTVTEATHDIPFDTTEIEGLVPFNYTNFTASTAANAASAVPQHTWAHVVDLDHKISDSEFSTFVPNMARKFPFELDTFQKEAVFHLEQGDSVFVAAHTSAGKTVVAEYAIAMAARNMTKTIYTSPIKALSNQKFRDFKQTFPDVDVGLITGDVQINPEATCLIMTTEILRSMVYRGADLIRDVEFVIFDEVHYVNDIDRGVVWEEVIIMLPDHVKYILLSATVPNTFEFANWVGRTKQKDIYVISTPKRPVPLEIFISAKGKMFKVIDGENRRFHEDEFRKHKDILEPKPKDDAPPTRGGRGGAARGGRGGAARGGARGSNRGGGGRGAPRGAARGGANKGRTPYNQDGPGKNTWLDLVHQLKPANLLPVVIFVFSKKKCEMYADSLQSVDFCNGREKSEIHMFIERSLGRLKKEDRNLPQIIKIREMLSRGIAVHHGGLLPIVKEFIEILFSKGLVKVLFATETFAMGLNLPTRTVVFSSLRKHDGRGFRNLLAGEFTQMSGRAGRRGLDKTGTVIIMTYNNPLSVTDFKEIALGTPTKLSSQFRLTYNMILNLLRIEALRVEEMIKYSFSENSTQVLLPENQKRYDELVDDLKNITITPCKHCHLQDVEQFCELLNQYEELYGQCVADIHDSPVLKTQILRIGRLVCFRDNTTLATRVGFVVKSVAANKAMLILTFDHGREYEEAAEKYKLPYIPIPTYINANFPKIVFSQGFRIASVPYEQITFVGKYSLRTPMNDIIANIPEAIQEASVAIKLICKYQNRFEEQDFKQVAQLSLHGLTNEKTNLLTKIEQMKSISTCRDFSLHYSQYHRQFMLTQQINQLQRLISDENLDLLPDYQQRLEVLETLGFIDEGQHTVVLKGRVACEINCGWELIITELILDNFLGDFEPAEIVALLSCFVYEGRTNEEEPPLITPRLEEGKSRILDIAKKLMQVYTEKQVSLTAEETDFLEAKRFALVNVVYEWASGLSFNEIMQQSVEAEGTIVRVITRLDEVCREVRNAALIVGDSGLYLKMGEAQEKIKRDIVFCASLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJR3</accession>
    <name>G3AJR3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aspartate aminotransferase</fullName>
            <ecNumber evidence="7">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">AAT2</name>
        <name evidence="9" type="ORF">SPAPADRAFT_59375</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="7"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373548.1">
        <property type="nucleotide sequence ID" value="XM_007373486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJR3"/>
    <dbReference type="FunCoup" id="G3AJR3">
        <property type="interactions" value="861"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJR3"/>
    <dbReference type="GeneID" id="18872885"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59375"/>
    <dbReference type="eggNOG" id="KOG1412">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032440_1_2_1"/>
    <dbReference type="InParanoid" id="G3AJR3"/>
    <dbReference type="OMA" id="GTWTHIT"/>
    <dbReference type="OrthoDB" id="6752799at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006532">
        <property type="term" value="P:aspartate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032938">
        <property type="term" value="P:negative regulation of translation in response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000064">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000001">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF55">
        <property type="entry name" value="GLUTAMATE OXALOACETATE TRANSAMINASE 1, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="8">
        <location>
            <begin position="29"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="415" mass="45870" checksum="97D2EC38F7B26C91" modified="2011-11-16" version="1">MSKFVGVTELPPDPLFGLKARYNADSRSDKVDLGIGAYRDNNGKPWILPAVKQAEQKLITSHDYNHEYLSISGFEPFFNAAAKVILGGDSLAIAEKRVVSQQSLSGTGALHLAGVFIKQFYNGNHTIYLSQPTWANHKQIFEYIGFKVASYPYWDNETKSLDLKGYLAAIEDAAPGSIFVLHACAHNPTGLDPNEAQWDQILKALEKKQHLVLFDSAYQGFASGDLNKDAHAIRHAINNRIITSPIVICQSFAKNCGMYGERVGAIHIIATSQPNDALNRAIKSQLNRIIRSELSNPPAYGSKIVAKILSDPQLYAQWEQDLITMSSRIHQMRAALRGKLEELGTPGTWDHITSQTGMFSFTGLNKEMVDRLEQVHGVYMVSSGRASVAGLNEGNVDRVAKAIDEVVRYHSKSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJU6</accession>
    <name>G3AJU6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_148328</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373581.1">
        <property type="nucleotide sequence ID" value="XM_007373519.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJU6"/>
    <dbReference type="FunCoup" id="G3AJU6">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="GeneID" id="18870780"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_148328"/>
    <dbReference type="eggNOG" id="ENOG502QPK1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_1_1"/>
    <dbReference type="InParanoid" id="G3AJU6"/>
    <dbReference type="OMA" id="RTTCMGF"/>
    <dbReference type="OrthoDB" id="5296287at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015355">
        <property type="term" value="F:secondary active monocarboxylate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035879">
        <property type="term" value="P:plasma membrane lactate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="357"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="452"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="74"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="512" mass="56770" checksum="1B7C7964EE2A4B49" modified="2011-11-16" version="1">MTSDTSSINPVEAKEDDKHVIHPPSFTWPAIRGYFSTRLSSLIVSRDEYSHYSLHEILNPFKPLVEMNLHQWNFFLLGFLAWTWDALDFFATSLNLTNIAEDLDVSVKDVSWGITLVLMLRTVGAIIFGALGDTRGRKWPYMINLFLLVVIQIGTGFVTTYQQFLGLRALFGVAMGGIFGICAAEALSDAPTKARGVLSGIFQEGYAFGYLLAVIFQRALVDTTPKGWRSLFWFSAGPPVLLIIWRFFTPETDAYLSQRAKFQEGAMNKSSKVKEFKSQAKAALKSYWLILIYLILMMTGFNFSSHGSQDLYPTMLTKQYGYGADASTVVNVCANLGALAGGIVIGHLSTFIGRRTAILCGNVITCAFIYPWAFKPMWVTAFFMQFGVQGSWSVVPIHLSELSPPQFRAFVSGVSYQLGNLVSSASSTIEATLGERFPIDPAKEIYDYGKTMAIFMAAVMVYLIIVILLGPENRGANLGGERKAVYVEESDDSEHEKADIKEIGIKTKVSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKC1</accession>
    <name>G3AKC1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Pre-mRNA-splicing factor CWC24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_55435</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the CWC24 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375095.1">
        <property type="nucleotide sequence ID" value="XM_007375033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKC1"/>
    <dbReference type="FunCoup" id="G3AKC1">
        <property type="interactions" value="304"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKC1"/>
    <dbReference type="GeneID" id="18871936"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55435"/>
    <dbReference type="eggNOG" id="KOG1813">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050460_3_0_1"/>
    <dbReference type="InParanoid" id="G3AKC1"/>
    <dbReference type="OMA" id="FICKTET"/>
    <dbReference type="OrthoDB" id="25761at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000349">
        <property type="term" value="P:generation of catalytic spliceosome for first transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034247">
        <property type="term" value="P:snoRNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039971">
        <property type="entry name" value="CWC24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12930:SF0">
        <property type="entry name" value="RING FINGER PROTEIN 113B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12930">
        <property type="entry name" value="ZINC FINGER PROTEIN 183"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="6 7 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 7 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="91"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="151"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="7">
        <location>
            <begin position="91"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="37"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW33580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="212" mass="24329" checksum="98C0572C3EAF0414" modified="2011-11-16" version="1">MFKKRTVKGKDDQSKKRRLEEDLNDEITTSIRQKTFKKVESASKHKPVSSTAEVLASNLQSNNEPATEVTPRTKGTIKAPPKNINVTTITDFQPDVCKDFLQTGYCGYGDTCKFLHIRDESRQKKPIEKEWENVTRKEIKDKPVEEIPFKCVLCKNEYKSPIKTQCGHLFCKPCFLDEYKQKKTKCFICKTETDGVMVPVSKSELENLIGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKD6</accession>
    <name>G3AKD6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">candidapepsin</fullName>
            <ecNumber evidence="4">3.4.23.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_150243</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential cleavage at the carboxyl of hydrophobic amino acids, but fails to cleave 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17 and 24-Phe-|-Phe-25 of insulin B chain. Activates trypsinogen, and degrades keratin.</text>
            <dbReference type="EC" id="3.4.23.24"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.24" evidence="4"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374408.1">
        <property type="nucleotide sequence ID" value="XM_007374346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKD6"/>
    <dbReference type="GeneID" id="18870859"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_150243"/>
    <dbReference type="eggNOG" id="KOG1339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013253_9_1_1"/>
    <dbReference type="InParanoid" id="G3AKD6"/>
    <dbReference type="OMA" id="NAVCIPK"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05474">
        <property type="entry name" value="SAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033876">
        <property type="entry name" value="SAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 13" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442418" description="candidapepsin" evidence="13">
        <location>
            <begin position="18"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="14">
        <location>
            <begin position="56"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW32893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="435" mass="47586" checksum="192788F773F2161F" modified="2011-11-16" version="1">MLKLLLLQWVLCGTAFAAVSPGYFSLEIRTSHVSKNGFGKRDAVDISLKDDKQSSMLTELQIGSNRDKVEVVVDTGSSVLWVMDPDVECKTSPLTGKVTDCKAHGTFSFQGSETFNKTEEPFHMEYEDKESADGFYVKDDISLGNDVDIKGFGFGLANGTSSDFGILGIRISFPEANSTGQSLPVELTNQGITNRSAYSIFLNKPNKKGSVLFGAVDHSKYLGDLQTVPIVYDETEPHIKVNVSGIDINMNGTKVSGGISESAFLLDTGTAISRFPKSFSDTLLERIEGSEYEDSVSFFGSLDCSQIENLKFEIKFDGKTITVPVKDLVYKKVHNSTDEGCGFMIKFEDDNDDLILGMDVLRSIYFVVDLDDKEVSLAQTDFSNGKQDIEAFPPPGVNRTLASRGRSEEKVSSANSIVSSIDVLLCLAISLTWLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKG3</accession>
    <name>G3AKG3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_60264</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374435.1">
        <property type="nucleotide sequence ID" value="XM_007374373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKG3"/>
    <dbReference type="STRING" id="619300.G3AKG3"/>
    <dbReference type="GeneID" id="18873303"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60264"/>
    <dbReference type="eggNOG" id="ENOG502S551">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016851_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKG3"/>
    <dbReference type="OMA" id="FNHKKHF"/>
    <dbReference type="OrthoDB" id="361494at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="330"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="564" mass="62145" checksum="933B67BA4561C1FC" modified="2011-11-16" version="1">MTDYYTPSLLFRQNAIRRYSPSLSPISSQTSLASTIESLHPNKNSWLLNLDNPKDSQVLTQSCSLNRVNIKSNYWKIPDTNMNLTGLAIAPNSPSVTPTLAISSGNRDANLFIYQLNLDENNLTHHCTITLPNIHAMEWVNNQYMITGNNKGYAHLVSIPSVTDAEENDEDAEICKRFNHRKHLKNHPDKYKSTAISKLAIHNNELISLYNNHLFQWDIKGVDTQTKPSPISITNISGISDMDTLAGSSTLAIAGKFGVSLFDSRDAKFKVPQYILPDSYEQFRELNANIIRWNSMDSNVLASGHGDGVVRLWDVRKINSCVASLCSSTSAEHTPTITTLEWNNGDIFTGAQDGNIIHWDLTSTPLSTSHTTCTLSEGLNSVKFDGRSNKIEIAQQRQCGTILPASNTNIVSMTSLQLDDDTVVVSIDGSSFLGVHSKVTEIKQYHTNLFDNTLVSSDTLVSVNEATQPLQIKEKRVTSSEPIIPVGINNVSVDTLQDVIVVEDGEDFHFGSNTDVDSCASLQVDSPNSSTHSVESLSTLATEVDQYNNKGINLMFNGIFDALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKJ2</accession>
    <name>G3AKJ2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">V-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_55450</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375112.1">
        <property type="nucleotide sequence ID" value="XM_007375050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKJ2"/>
    <dbReference type="STRING" id="619300.G3AKJ2"/>
    <dbReference type="GeneID" id="18871937"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55450"/>
    <dbReference type="eggNOG" id="KOG0859">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087338_1_0_1"/>
    <dbReference type="InParanoid" id="G3AKJ2"/>
    <dbReference type="OMA" id="IAHVHAN"/>
    <dbReference type="OrthoDB" id="190375at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15843">
        <property type="entry name" value="R-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000004">
        <property type="entry name" value="Vesicle-associated membrane protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051097">
        <property type="entry name" value="Synaptobrevin-like_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136">
        <property type="entry name" value="SNARE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136:SF168">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="237"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="10">
        <location>
            <begin position="173"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="269" mass="30956" checksum="539C8D6E1052B3CB" modified="2011-11-16" version="1">MSHSDLYKHVIHVTLTVNSTTLYTYENHQLIPLLNNLNCSELISQELGLINSSKTLIGSVPLSASSNYKLVLYFIKKVIANSDGSRDLITVVVICHYSMNKTLVLNILKTIMDKYIEFRKRIEEESSGKNDKLKLGEFKLYMNQIIKYEEMNYESNQNLYNYGSTEETINPNQLLLVNEEVDEVRQLMLDNINKLLSRGDKINLLVDQTDRLNTSSSMFQRKAQQIKRKMWINKAKFILMLIGGIILLVYLVIGSQCGFPFFGSCRHNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKP7</accession>
    <name>G3AKP7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_150389</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3B4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374466.1">
        <property type="nucleotide sequence ID" value="XM_007374404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKP7"/>
    <dbReference type="FunCoup" id="G3AKP7">
        <property type="interactions" value="339"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKP7"/>
    <dbReference type="GeneID" id="18870866"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_150389"/>
    <dbReference type="eggNOG" id="KOG0131">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_21_2_1"/>
    <dbReference type="InParanoid" id="G3AKP7"/>
    <dbReference type="OMA" id="IVWELMI"/>
    <dbReference type="OrthoDB" id="10259687at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048026">
        <property type="term" value="P:positive regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12334">
        <property type="entry name" value="RRM1_SF3B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000895">
        <property type="entry name" value="Hsh49p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000505">
        <property type="entry name" value="Splicing factor 3B subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034158">
        <property type="entry name" value="SF3B4_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052084">
        <property type="entry name" value="SF3B4_spliceosome_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030:SF3">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="29"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="138"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="256" mass="28978" checksum="9FE3837C1ADABC20" modified="2011-11-16" version="1">MHILITQTATPTMTTIFRKLQDSDRNPNATLYFGNIDPRATELLMYELFIQFGPVRSINMPKDRILRTHQGYGFVEFRSSKDAEYVLDILRGVRLYGKLVKLKKVDGKESSKRTQIGREGRGNVTESVVLPGYVDVGAKLFINNLNELVDEKFLSDTFGKFGTLIQTPIVKRDDEGKSLGFAFLNYDSFNSSDLAIEKMNGVILMNSKISVGYAFKSESGGKRIRHGDKVERLLAESAKQNNVVKKTKKRKVDKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKR2</accession>
    <name>G3AKR2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">Proteasome assembly chaperone 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_60298</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in 20S proteasome assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the 20S proteasome chaperone.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the PSMG2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374481.1">
        <property type="nucleotide sequence ID" value="XM_007374419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKR2"/>
    <dbReference type="FunCoup" id="G3AKR2">
        <property type="interactions" value="123"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKR2"/>
    <dbReference type="GeneID" id="18873328"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60298"/>
    <dbReference type="eggNOG" id="KOG3112">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062640_2_1_1"/>
    <dbReference type="InParanoid" id="G3AKR2"/>
    <dbReference type="OMA" id="PISWKGV"/>
    <dbReference type="OrthoDB" id="10260712at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016562">
        <property type="entry name" value="Proteasome_assmbl_chp_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970:SF1">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010044">
        <property type="entry name" value="UCP010044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="261" mass="29153" checksum="B6FC3A84BE3AFF53" modified="2011-11-16" version="1">MSSFISIAEGTQPDLSSTTLIIPSISIGNIPQLAIDLLIHTFSFAKVGSLDDLYVYPFASPVDYSKTPVPGISRAIEVYHCPELQLTLVQQRSPILPSYTDVFVKETILPFIKAFKFARVVVLDSSDAGLVEHVHAGGIEVYTTEVLLSKSLESLKLSHETQELQELEAQEQQKHSNYVRSLLKAFNLPRIDKNVEVNVDFMVLVSFVYEGDNFYDAEKLADKLGEVLRLKKVDRWTRPVSWFGAYGDKPVPDAVEEGLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL23</accession>
    <name>G3AL23_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_60382</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374581.1">
        <property type="nucleotide sequence ID" value="XM_007374519.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL23"/>
    <dbReference type="GeneID" id="18873379"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60382"/>
    <dbReference type="HOGENOM" id="CLU_2127075_0_0_1"/>
    <dbReference type="InParanoid" id="G3AL23"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="114" mass="13318" checksum="2C29BB3340C1B1E1" modified="2011-11-16" version="1" fragment="single">MATSRPIQETITELEGMLDNINHLIEDKLPKSLHRSHIESSNAEQFKKQLDDYDTFLTEIKQLLSTSEVIDHQLLWLDSLGYLAGILLKLKLLFGRFYLHIPFYEIEPIFEMAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL36</accession>
    <name>G3AL36_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sulfite reductase [NADPH] subunit beta</fullName>
            <ecNumber evidence="6">1.8.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">SPAPADRAFT_66085</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the reduction of sulfite to sulfide, one of several activities required for the biosynthesis of L-cysteine from sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>hydrogen sulfide + 3 NADP(+) + 3 H2O = sulfite + 3 NADPH + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>siroheme</name>
            <dbReference type="ChEBI" id="CHEBI:60052"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Alpha(2)-beta(2). The alpha component is a flavoprotein, the beta component is a hemoprotein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.2" evidence="6"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374593.1">
        <property type="nucleotide sequence ID" value="XM_007374531.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AL36">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL36"/>
    <dbReference type="GeneID" id="18875080"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66085"/>
    <dbReference type="eggNOG" id="KOG0560">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001975_2_1_1"/>
    <dbReference type="InParanoid" id="G3AL36"/>
    <dbReference type="OMA" id="YGSYTQV"/>
    <dbReference type="OrthoDB" id="1688044at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009337">
        <property type="term" value="C:sulfite reductase complex (NADPH)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050311">
        <property type="term" value="F:sulfite reductase (ferredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004783">
        <property type="term" value="F:sulfite reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000097">
        <property type="term" value="P:sulfur amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.413.10:FF:000003">
        <property type="entry name" value="Sulfite reductase [NADPH] hemoprotein beta-component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.413.10:FF:000004">
        <property type="entry name" value="Sulfite reductase [NADPH] hemoprotein beta-component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000051">
        <property type="entry name" value="Sulfite reductase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000016">
        <property type="entry name" value="Sulfite reductase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="entry name" value="Sulfite Reductase Hemoprotein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
        <property type="entry name" value="Nit/Sulf_reduc_fer-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006067">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045169">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006066">
        <property type="entry name" value="NO2/SO3_Rdtase_FeS/sirohaem_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010029">
        <property type="entry name" value="PRK13504.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11493:SF47">
        <property type="entry name" value="SULFITE REDUCTASE [NADPH] SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11493">
        <property type="entry name" value="SULFITE REDUCTASE [NADPH] SUBUNIT BETA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03460">
        <property type="entry name" value="NIR_SIR_ferr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00397">
        <property type="entry name" value="SIROHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="Nitrite and sulphite reductase 4Fe-4S domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55124">
        <property type="entry name" value="Nitrite/Sulfite reductase N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00365">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0349">Heme</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="20">
        <location>
            <begin position="676"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1019"/>
            <end position="1040"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00057613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00067595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EGW33078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1432" mass="159392" checksum="7CC74772D74F7A89" modified="2011-11-16" version="1">MSINQQQAIAHTVNAIGGTVFVTQPAKSVESSFTSVLPQATTLVNSNDPFTIIQEYLDQAQDNNEFVTVATDGVTLFNSLPHLSSSAFKSNKVVAHVELNNFDFAIVTSLRDLHFPVLVSQTANEASKFATVAYNLSENLNTFVFHFFAPTTYLVNESVKLPSEILLESVQDYSVEHIISHYEVKPFEIVNKVAKQKDTILYLGQLSDALVEAATKADALLISIKLYQPFQLAQLLKIIPSSVETLTIVQQTTTPFASFSPVLLDFFQQYPEYQTLKTFTKIVSATFGYIKSEGYQQAINNLLANVRSESTVQNLSFGKVNTELATKSSKKQQDDLAGAVAEAHSLEQAYLKVLQQVNPDNLNVLNEFQAENIGLETNPEFGFGSFLYDQEQRENLISLVQNAVKKNQFKSGNDTKLIELLSTWLVQVREVGVVEAKLSQEIVALLETDKTSTIARELLALSQYFSQEKNWLIGSDAWAYDLGSSGVHNVITSGKNINMLIIDSEPYAEKNQEKTINGFRKKDVGLYAMNYGGCYVASVAIYSSYTQVLQAFLEAEKFQGPSIVLAYLPYKKESDDALAVLQETKKAVDSGYWPLYRYDPTIENEADIFKLDSSNLRKQLQDFLERENRLTLLATKDPLLSRNLTATANTEAKAQQKKIADESFAKLLQGLSGPPLTVAFASDGGNAEAVAKKINRQALGRGLKSTVLQMDDIAVEDLPTETNIVFVTSTSGQGEFPTNGKQFWDGIKNSVDLDLASVKFSVFGLGDSQYWPRKEDKHYYNKPAKDLWAKLKLYGGVELADIGLGDDQDADGFSTGFNEWIEKIWAALGVDNVEGVEEPKPITNEDMKLGSNYLRGTIVEGLNDQSTGAISAVDQQLTKFHGIYMQDDRDIRDDRKAQGLEPAYAFMVRARLPGGIATPKQYLRLDELSDVRGNGTLKLTTRATFQLHGVVKHNLKPSIRGMNSVLMDTLAACGDVNRNVMVSALPHNAKIHTQVSQVGALISEHLLPRTTAYHEIWLQGEDDGDKPGDSANWENRKEGPAKKKTLVAGNALEDFEPMYGKVYLPRKFKVVITVPPYNDVDVYAHDIGLIAIVEDNEVVGFNVLVGGGMGSTHNNKKTYPRTGSMFGYVAKDQIHLVCEKIMLVQRDFGDRTNRKHARLKYTIDDLGVDVYKSKVEELLGWKFAEPREFKIESNVDYFGWCKDELGFNHFTAFIENGRIEDTAELPQKTGLKKIAQFLETKKSGEFRLTGNQHLVISNIDDADLNVVKSLLAKYKLDNTDFSALRLSSAACVAFPTCGLAMAESERYLPVLISKLELALEEYGLRHDSVVMRMTGCPNGCARPWLAEVALVGKAYGAYNLLLGGGHHGQRLNKIYRYSIKEDEILDILKPLFKRWSLEREDGEPFGDWCIRAGIIKETTEGKYFHDDIPEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALH5</accession>
    <name>G3ALH5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_50124</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374733.1">
        <property type="nucleotide sequence ID" value="XM_007374671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALH5"/>
    <dbReference type="FunCoup" id="G3ALH5">
        <property type="interactions" value="1130"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALH5"/>
    <dbReference type="GeneID" id="18871406"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50124"/>
    <dbReference type="eggNOG" id="KOG3301">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089738_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALH5"/>
    <dbReference type="OMA" id="RQFITHG"/>
    <dbReference type="OrthoDB" id="1697570at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000021">
        <property type="entry name" value="40S ribosomal protein S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_uS4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_uS4_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003139">
        <property type="entry name" value="PRK04051.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF5">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="10">
        <location>
            <begin position="6"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="RNA-binding S4" evidence="9">
        <location>
            <begin position="107"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="188" mass="21739" checksum="8C1A4EDE66AE1C74" modified="2011-11-16" version="1">MPRAPRTYSKTYTVPKRPYESARLDAELKLAGEYGLKNKREIYRIGYQLSKIRRAARDLLTRDEKDPKRLFEGNALIRRLVRVGVLSEDKMKLDYVLALRIEDFLERRLQTQVFKLGLARSIHHARVLISQRHIAVGKQIVNIPSFMVRLDSQKHIDFAHNSPYGGGRAGRVKRKNQGKGEEAAEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALM1</accession>
    <name>G3ALM1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar protein sorting-associated protein 74</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_50163</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Mediates the cis and medial Golgi localization of mannosyltransferases through direct binding of their cytosolic domains. Involved in vacuolar protein sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">Golgi stack membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GOLPH3/VPS74 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374780.1">
        <property type="nucleotide sequence ID" value="XM_007374718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALM1"/>
    <dbReference type="FunCoup" id="G3ALM1">
        <property type="interactions" value="563"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALM1"/>
    <dbReference type="GeneID" id="18871412"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50163"/>
    <dbReference type="eggNOG" id="KOG3983">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036311_1_1_1"/>
    <dbReference type="InParanoid" id="G3ALM1"/>
    <dbReference type="OMA" id="GETWNLL"/>
    <dbReference type="OrthoDB" id="76388at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048194">
        <property type="term" value="P:Golgi vesicle budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060304">
        <property type="term" value="P:regulation of phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3630.10:FF:000002">
        <property type="entry name" value="Vacuolar sorting-associated 74 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3630.10">
        <property type="entry name" value="yeast vps74-n-term truncation variant domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008628">
        <property type="entry name" value="GPP34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038261">
        <property type="entry name" value="GPP34-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12704:SF2">
        <property type="entry name" value="GOLGI PHOSPHOPROTEIN 3 HOMOLOG SAURON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12704">
        <property type="entry name" value="TRANS-GOLGI PROTEIN GMX33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05719">
        <property type="entry name" value="GPP34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="348" mass="39370" checksum="D5555E000B139894" modified="2011-11-16" version="1">MSSSGLQRRRGAGKSSTPKPRTSDSFDEDRTANSGVGPASSENHHKIGYDSRDIEDRDTNLKIPLLTLMEEVLLIGLKDKEGYLSFWNDNISYTLRGCILLELAFRNKITLVNDPARRRFELSDRLVEVIDSEPTGEVLLDEALKIMKGDESNLSVTNWIDLLSGETWNLMKINYQLKQVRERLAKGLVDKGILRTERKNFFLFDMATHPVADKLSKEFIKNRILNLLVQRSVDLDAVANDQFPKDTSYRYLRSISLVCGAYAANVLENVLLSLNYDKRDQAFARADELLNDFSEYPFNLRQNQSPLGVGLNLGQEVNDEIEKSSASELQLELIAAVLSVFARMDSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALM2</accession>
    <name>G3ALM2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">chitinase</fullName>
            <ecNumber evidence="3">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_60603</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="3"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374779.1">
        <property type="nucleotide sequence ID" value="XM_007374717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALM2"/>
    <dbReference type="FunCoup" id="G3ALM2">
        <property type="interactions" value="589"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALM2"/>
    <dbReference type="GeneID" id="18873486"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60603"/>
    <dbReference type="eggNOG" id="KOG2806">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002833_1_0_1"/>
    <dbReference type="InParanoid" id="G3ALM2"/>
    <dbReference type="OMA" id="WMGNFTA"/>
    <dbReference type="OrthoDB" id="76388at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035885">
        <property type="term" value="F:exochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06548">
        <property type="entry name" value="GH18_chitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000075">
        <property type="entry name" value="Sporulation-specific chitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF317">
        <property type="entry name" value="CHITINASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="8 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="GH18" evidence="12">
        <location>
            <begin position="16"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW33264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="392" mass="44439" checksum="2C001843D68C987A" modified="2011-11-16" version="1">MLFHNHQQPLKAPNGYKTEVYFSNWSVYQRSHFAIDIPIKYYTHIFYAFILVDQQTGRLKFSDEWCDLQMPLKSATGAPVVGNLQQLFSMKLANRHLKVIMSIGGWGTCHLFEAVMSDKQKLANFIDSAVEFVHEYGFDGVDIDWEYPKNDKDARNYVELLRGLRSKLPNQFSLSIAAPAGAENVMVLRIKEMDQYLSFWNLMCYDFAGEGWSKRTGFHSNLFGNNGDNELNADNVVEHYKRMGVDSSKLILGMPMYGRVFHGVAKPEIGHSFSSSVRKGPDVIDTEIVDYKRIPLDTEVFDPRKVSACNYTGSSQQFIVYDNAQSARIKAGYTRSKQLGGGMWWDSCGDSPDPAKSLVINYVDQLGGIDVLDKSDNVVSYPTSKYLKDLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALR8</accession>
    <name>G3ALR8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60651</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374826.1">
        <property type="nucleotide sequence ID" value="XM_007374764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALR8"/>
    <dbReference type="FunCoup" id="G3ALR8">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALR8"/>
    <dbReference type="GeneID" id="18873505"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60651"/>
    <dbReference type="eggNOG" id="KOG4781">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052827_2_2_1"/>
    <dbReference type="InParanoid" id="G3ALR8"/>
    <dbReference type="OMA" id="GRKCIIT"/>
    <dbReference type="OrthoDB" id="506431at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052061">
        <property type="entry name" value="PTE-AB_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260:SF4">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260">
        <property type="entry name" value="UPF0644 PROTEIN PB2B4.06"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="1">
        <location>
            <begin position="122"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="224" mass="25107" checksum="59416706641634FA" modified="2011-11-16" version="1">MSFRGFVCKTIPIVAFGVGIASFPRSWRKEYKGDPKLLRINESVLNQIQDTELYKTLQTNNNFKQYNSSHAFPGQHHSNYIGTGLLFGPDLFEIDPIVFLNEEKGELVSFYHLGESLISQDGQIHNGVTSTILDEGLCSAGFPLLPAKKGVTASLSIDFKNQAPPRSTVILRAKVKEAKGRKVVIEGYLETLSFDLSVKPTRVADAKCVLVQPKWFKYLQWLQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALS1</accession>
    <name>G3ALS1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_136404</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374829.1">
        <property type="nucleotide sequence ID" value="XM_007374767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALS1"/>
    <dbReference type="STRING" id="619300.G3ALS1"/>
    <dbReference type="GeneID" id="18869966"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136404"/>
    <dbReference type="eggNOG" id="KOG0198">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001872_2_1_1"/>
    <dbReference type="InParanoid" id="G3ALS1"/>
    <dbReference type="OMA" id="HDLNPMA"/>
    <dbReference type="OrthoDB" id="8693905at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06627">
        <property type="entry name" value="STKc_Cdc7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF26">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DDB_G0283821-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="196"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1113" mass="126466" checksum="F87E84D25B5FC6C1" modified="2011-11-16" version="1">MGEITTHVSPNVTTNDKKESTLAKYRETDDDDLDAGDFDLNLSFSKFSQVSQGSVIVKSSSKTEESESGSSDSPFVQRISNKVEVQFDNGFVSTYDKIQNGRVVYPVQEVSGSNDENQESPFLDSEANKLDAFDNNIKQIRPQKLRNKLFSNVVAPKTNIGTPKHQRTSSVKVSLTPAQKFEKKTTTHSDNALDNFEFGTLVGNGAFASVYKGTNLKTNQVVAIKQIRLEKDQDVAVLMGEIDLLKILKHPNIVKYHGFVKTSTSLNVLLEYCAGGSLRQLYKKMKRGLPESQIINYVRQILHGLTYLHDQGVVHRDVKAANVLLTDTGDVKLADFGVATRVNASHYTVVGTPNWMAPETVIGGDGLCTASDIWSLGATIIELFTMNPPYHDLNPMATLHAIGTDEHPPLPKGLSSLAKNFLLECFQKQPNLRISAKLLLKHKWLNEGITTKSSLMNLQAKRQPSMELKSILSYQEQNDDENWDNDFSEMKVLKVKGMSPDVIHLEDIESEFETEFEEPVKYTKAELLTKFAEDNSDFSDLDHIDSKKLVNHEADEADIGDSDPFSNMEIDSFDTNDLEVQSKMEYLVSRYSRKLDQARMENDEAIASLVKITGRMLHLIKKYPVSHVTFIREHGVLSILDLLDSYQEIPRQQQLWYHALSILNYMFESNVSSFENFCFLGGIPAIAHFRNVSYDIQVRLQVVRFVRLLNTSDKALSMFVSCGGFRLVAKFVEEDFDTTPTFPLVAIECIHNVLSKDLIRSKSDMCRILSKHGLVFWFMVLLNRLLKLDRDQTHKSVSSEEIRITIDRIVDIIKHFGQSEIRVRITIANTDVFKLMIKVYDQLNHDHKIAILKFIKSMSCVSEVLKYLYRADILEFMLRLLQTNTPSNRNYKEYINVVAPILYNCLSLNYIRESEFVELGALPYLKNLSIINLPFKQFILPIVCELAYCEEKVRNEMKKYDILSVYYNLLFDPYWQPNALESIYNWYRSDPAYVNLEAPRAMDCLVAGFLLPKVSNLESALDIYLQLISINAEITKLMSNSNVINNILMKLNVHKKNPVIQLLLLKILKWLLQSCDTLSVNVSTSVANSLHAITRSSSSVLIEELVIEILELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALV9</accession>
    <name>G3ALV9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60075</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="eggNOG" id="ENOG502RQK3">
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="79" mass="9072" checksum="0C6933D568F7EAD2" modified="2011-11-16" version="1">MKQPPAVKRLFGLYFHHFPFVMLTTTEAVFLHTSILIFAMFIAYGIYSYLPATIMFSISRASYYLFGESNTLGANTLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
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    <name>G3AME0_SPAPN</name>
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        </submittedName>
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        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007374245.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="G3AME0">
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    </dbReference>
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    <dbReference type="eggNOG" id="KOG0310">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="C:small-subunit processome"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034455">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:U3 snoRNA binding"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="UTP15p Nucleolar protein"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW32792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="354" mass="39261" checksum="61AB6D68200269F2" modified="2011-11-16" version="1" fragment="single">MSATRQAIPQVRNPTLPSQTTPEQRYWRSYVSPQLIKENHPVNCIDFNPITPNDFAITSSTRIQIFSSKTRQVIKTFSRFKDIVYSGKFRYDGKLLVAADATGLVSIYDSYQPRNLLVSLHPSSHPTHVAKFHPTIGNQLITGSDDRILRLYDISQTTKGPIVEFDGSHHTDYIRSANFIPGNPNLIATGCYDGIVRIFDTRNFSTSQHQVVAQFNQHNPIEDVLAISSTTLVSAGGPQVRIWDLTRGAQIHELNNFTKTATSLHDTGDRGLLVGSLDGHVKIFDYTSSNWDVQFGWKFGSGGVLSCGVSPASNDHKHFVAGLTSGLISIRTKKTEPKVKQGVKQEKSAAYARM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMF4</accession>
    <name>G3AMF4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">SPAPADRAFT_60151</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007374260.1"/>
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    </dbReference>
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    <dbReference type="eggNOG" id="KOG0191">
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    <dbReference type="InParanoid" id="G3AMF4"/>
    <dbReference type="OrthoDB" id="72053at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="388"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="250"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="433" mass="50695" checksum="6D9D34A81D14A041" modified="2011-11-16" version="1">MFLPKAARIHQVNYDGPIEINAMKKWAVKLGANSKYEVVTKKTLGSHMKLVDKLEPGKINHDGKNAVSVVFYFDEVSVTPEDESVLTHLLLELQKSPFNTRLLISKHKQLEEHVNSMSKELIKYINYDAKAPEYKYNKPMEIATTLTAKPTILVFKDNTLMVDIYQTYAPEDIRIFGRVEDFIRRSQFPTYHELTPDLLPSYFTKFSDREYKVVIAFVDSKKDITTDLDNLVLAAHEYHHLKKEYYYHKLESDRQRKQKWIDELKKQNTESVKVIEAMRKEIPHLWNNDDVLFTYIDKSKASEFNYIPGWKLDIDQYDSGDAIVLSKDYRYYWDHDVKGQKLKNDPATMKHVLLSLLDPRLENSNVVRRVTGSPYSTQFQFMDHIHDYGIFGYLALFSVVYLLGYFFVRRRRRRHRTGNGNTGIIGAAVPKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMJ2</accession>
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            <fullName evidence="12">Yos1-like protein</fullName>
        </recommendedName>
    </protein>
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    <organism evidence="11">
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pichiomycetes</taxon>
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        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the YOS1 family.</text>
    </comment>
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        <property type="protein sequence ID" value="EGW33438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374953.1">
        <property type="nucleotide sequence ID" value="XM_007374891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMJ2"/>
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        <property type="interactions" value="265"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMJ2"/>
    <dbReference type="GeneID" id="18869938"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136027"/>
    <dbReference type="eggNOG" id="KOG4779">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152125_0_1_1"/>
    <dbReference type="InParanoid" id="G3AMJ2"/>
    <dbReference type="OMA" id="VQTVMRM"/>
    <dbReference type="OrthoDB" id="8119704at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013880">
        <property type="entry name" value="Yos1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15858">
        <property type="entry name" value="IMMEDIATE EARLY RESPONSE 3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15858:SF0">
        <property type="entry name" value="IMMEDIATE EARLY RESPONSE 3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08571">
        <property type="entry name" value="Yos1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442607" description="Yos1-like protein" evidence="9">
        <location>
            <begin position="23"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="10681" checksum="E5F094FBC3B77A63" modified="2011-11-16" version="1">MFGLGKLLYVVVLTINGIAVLSEERFLNRIGWGSRRYDQQANIQFNQFNTGIAVNDEGSIKNKIINLINATRTLMRIPLIVLNIFIILYELILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMS5</accession>
    <name>G3AMS5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_151749</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Acts as one of several non-catalytic accessory components of the cytoplasmic dynein complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Cytoplasmic dynein consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits which present intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cytoplasm</location>
            <location evidence="1 15">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the dynein light chain family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375034.1">
        <property type="nucleotide sequence ID" value="XM_007374972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMS5"/>
    <dbReference type="FunCoup" id="G3AMS5">
        <property type="interactions" value="683"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMS5"/>
    <dbReference type="GeneID" id="18870904"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_151749"/>
    <dbReference type="eggNOG" id="KOG3430">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070944_4_0_1"/>
    <dbReference type="InParanoid" id="G3AMS5"/>
    <dbReference type="OMA" id="THEKGHF"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040001">
        <property type="term" value="P:establishment of mitotic spindle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051292">
        <property type="term" value="P:nuclear pore complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21452">
        <property type="entry name" value="DLC-like_DYNLL1_DYNLL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.740.10:FF:000005">
        <property type="entry name" value="Dynein light chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.740.10">
        <property type="entry name" value="Protein Inhibitor Of Neuronal Nitric Oxide Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037177">
        <property type="entry name" value="DLC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019763">
        <property type="entry name" value="Dynein_light_1/2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886:SF35">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01375">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54648">
        <property type="entry name" value="DLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01239">
        <property type="entry name" value="DYNEIN_LIGHT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 15" id="KW-0243">Dynein</keyword>
    <keyword evidence="6 15" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12 15" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="11" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU365010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW33519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="93" mass="10712" checksum="6192CE460C88FC81" modified="2011-11-16" version="1">MTMQEPEHTPTLKASDLSEEIQTKIFELAQQSIVTCKIEKDIATFLKKELDQIYGPTWHVIVGRSFGSYVTHEQGYFIYFYIGDLAFLIFKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMV2</accession>
    <name>G3AMV2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_61447</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375642.1">
        <property type="nucleotide sequence ID" value="XM_007375580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMV2"/>
    <dbReference type="FunCoup" id="G3AMV2">
        <property type="interactions" value="477"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMV2"/>
    <dbReference type="GeneID" id="18873898"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61447"/>
    <dbReference type="eggNOG" id="KOG1716">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_694774_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMV2"/>
    <dbReference type="OrthoDB" id="426001at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14521">
        <property type="entry name" value="DSP_fungal_SDP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="213"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="280"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="24"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="397" mass="44064" checksum="A71DBDCCF4BD5692" modified="2011-11-16" version="1">MSSPSITKHGPIITAGPLTKLSLSIPDYPRPDGTSAVTLRPFHDDMTTPLVTQTPTMPPALNPQVHTNTPAEYRFPPSSPPQLDQRRHSGPNKRHSNRSSSQSLDMNVFMANRNDSFDHSTVQPPPAPPFAPGMKGSPLSTPPRLTSPLKLHGISPETPRSLTDALARVSMTTNEDYNDSMLSNTPQSLFTTYKEMIPEELQESNSLDAYPNGPRSVLNNRIFLYSDPLTSKKQIDINEYNLVINVAKECKDMSANYLNPIPGKREYLYLPWLHTSQISKDLAPIMTKIDAFYNMGYKILIHCQCGVSRSACVVVAYYMMKFTLGVNDAYELLKNGTNGNREVCMSVRNGGNIIDGSDRICPNMSLIFELMEFGDSLCHKEVTTSALLFDSPSTMQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMW4</accession>
    <name>G3AMW4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_55840</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375654.1">
        <property type="nucleotide sequence ID" value="XM_007375592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMW4"/>
    <dbReference type="FunCoup" id="G3AMW4">
        <property type="interactions" value="274"/>
    </dbReference>
    <dbReference type="GeneID" id="18871977"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55840"/>
    <dbReference type="eggNOG" id="ENOG502QQBG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009030_1_0_1"/>
    <dbReference type="InParanoid" id="G3AMW4"/>
    <dbReference type="OMA" id="ARQIMFE"/>
    <dbReference type="OrthoDB" id="3477330at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000821">
        <property type="term" value="P:regulation of arginine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF32">
        <property type="entry name" value="ARGININE METABOLISM REGULATION PROTEIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="41"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="184"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="312"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="223"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="312"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="325"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="944" mass="106076" checksum="F7291C822C7D696E" modified="2011-11-16" version="1">MESVDIERSPTVEPDGNERNSNKTNGTNNGSKAKRSKTFTGCFTCRSRKIRCDLEKPACMKCTKAGLICSGYDIKLRWSLPIEFNSKNGSAYSKSVVFKSKSKKSAKGSLDEDDEEEGNGMDFFQRRQVGFVVWDEKRNCKPYETYEDMDRDLGILHNSGNDQLVNSEGKTKLLGPFGVFKGKGALKRSQSKDRSEKSLKRQRGIEKSARTPLNKSLLHSSASTPTTTTGTPGMSMYMDSTAPHDQLWLSNELRDDALLTAAALDTQFLDFMNAGNNILPPNTNQRSYNSGYNFNNPNDLLNLVFHKNQSMSGPALASHTQPQPSNSHMSMSNLLNDTNSSNTMIDPTNQHLYYSNYNDYYYNNPYSTNETLQIHLHASPKVNADDDAETTDLVDGENAHSTHMPTSIMSIVQSPLKLGFDLSFPATSSTSPQVGIPTTALQVQPLTRYLLNYYVNQVADLMTVIPLTENPWKTIYFPRALMAIGELSALGRTSTAKNALLNALLAVSAFNLQSKFPKNSQPMKYYLNLGIALRNQASLFVKQLLNTKSDTQSGIEYCLSTEKYKDVLCAVMTMISVDLVWGTMQDTGVYITWCGKVILAKMANKKKLSSKARILHRIFSSLKLIQDSTCLALDSIKEDFESNVGSGYDVNGDKFANTARNTASGKARIDFIVNNSFSSATVPSPSKKSASPSFVNKKLINTKKNDENFATDALYGLPNSLIILFSDTVELLRTKIYYKETKRKLPDSFPTRVEVLNKKLINWKLDWELYQNQNDKTTTTKASIVVDDDLEDDNMNNVEVELLSDSKKKFYSPMHQATYHHIMSFYHALMIYFNRLIQEIPPVKLQTKVAKTLNHLNAIQKLIAKGEAAIIPLFWQGFIAGCEAISLNLQLGFKKWGADIAQYLGSYWGARQIMLEVWRRKRMHEARDDWVSVVQDWEMNLMLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN93</accession>
    <name>G3AN93_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mannose-1-phosphate guanylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_61543</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>alpha-D-mannose 1-phosphate + GTP + H(+) = GDP-alpha-D-mannose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="EC" id="2.7.7.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.13" evidence="3"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375752.1">
        <property type="nucleotide sequence ID" value="XM_007375690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN93"/>
    <dbReference type="STRING" id="619300.G3AN93"/>
    <dbReference type="GeneID" id="18873944"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61543"/>
    <dbReference type="eggNOG" id="KOG1460">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_3_0_1"/>
    <dbReference type="InParanoid" id="G3AN93"/>
    <dbReference type="OMA" id="MPVPNWW"/>
    <dbReference type="OrthoDB" id="285674at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004475">
        <property type="term" value="F:mannose-1-phosphate guanylyltransferase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="5">
        <location>
            <begin position="4"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="6">
        <location>
            <begin position="327"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="447" mass="49466" checksum="F01A417BF4B9BB97" modified="2011-11-16" version="1">MALKVLILVGGETTGTRFRPLSMEGPKLLFPICGKPLITHIIDSLVEQLSDEISEILLIGFFKDSSVFQNYIKQVNSVYPKLKIKYLTEPKPLGTAGGLYHFREEIFTSQDCKLLMIHGDVICDYPFKKMLESYDKEKSNITLFGLDPVTFTKKSSALSNASGEVPDDEFILQNYGAIVAKRSTSDIIHYVEKPSSTISMFRQDTKYRILLNGGIYIFDRSIVDLLQEAKIKKETSNQLVDDVDLEDSKSNVLSLELDVFKTLPQVPETKFTVYQSDDFWYQLKSPVSALLANNFFLAKGACNTSVSTTVEILKPVQILDSDLSQAGSSKIGPNVSIGKNVTIGSGVRLANCIIADDVSIGDNTIIKNAIVSSGAKIGKWCRIEGTITSQTLAENANPNASEGYLKLLNNIVILCQGTVVKSQVFVYNSVVLPHKELKNDIKYEIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AND1</accession>
    <name>G3AND1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_61576</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL27 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375790.1">
        <property type="nucleotide sequence ID" value="XM_007375728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AND1"/>
    <dbReference type="FunCoup" id="G3AND1">
        <property type="interactions" value="1137"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AND1"/>
    <dbReference type="GeneID" id="18873962"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61576"/>
    <dbReference type="eggNOG" id="KOG3418">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067359_0_1_1"/>
    <dbReference type="InParanoid" id="G3AND1"/>
    <dbReference type="OMA" id="NQWFFTK"/>
    <dbReference type="OrthoDB" id="2365484at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06090">
        <property type="entry name" value="KOW_RPL27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.770:FF:000001">
        <property type="entry name" value="60S ribosomal protein L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001141">
        <property type="entry name" value="Ribosomal_eL27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018262">
        <property type="entry name" value="Ribosomal_eL27_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041991">
        <property type="entry name" value="Ribosomal_eL27_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038655">
        <property type="entry name" value="Ribosomal_eL27_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10497">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01777">
        <property type="entry name" value="Ribosomal_L27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01107">
        <property type="entry name" value="RIBOSOMAL_L27E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="136" mass="15380" checksum="A505806DD6184A78" modified="2011-11-16" version="1">MAKFIKSGKVAIVVRGRFAGKKVVIVKPHDEGTKSHPFPHAIVVGVERNPLKVTKNLGAKKTAKRTKVKPFVKLVNYNHLMPTRYSLDVESFKSAVTSEALEEPSQREEAKKVVKKALEEKNQAGKNKWFFQKLHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANW2</accession>
    <name>G3ANW2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bola-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_139706</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BolA/IbaG family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375863.1">
        <property type="nucleotide sequence ID" value="XM_007375801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANW2"/>
    <dbReference type="FunCoup" id="G3ANW2">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANW2"/>
    <dbReference type="GeneID" id="18870227"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139706"/>
    <dbReference type="eggNOG" id="KOG2313">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109462_2_0_1"/>
    <dbReference type="InParanoid" id="G3ANW2"/>
    <dbReference type="OMA" id="FNDSYKH"/>
    <dbReference type="OrthoDB" id="411584at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044572">
        <property type="term" value="P:[4Fe-4S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46230:SF7">
        <property type="entry name" value="BOLA-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003113">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="13408" checksum="2E060B9085A9C188" modified="2011-11-16" version="1">MFKQLLRTMTTTAKLIHSETPGPIEISILTKIQDSFSPTYLKIVNDSYKHAHHAGIRGATNTTESHFNIEIVSSIFEGKTLPTRHRLVYNLLDDEIKHKGVHALQLRTKTESEINKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANZ7</accession>
    <name>G3ANZ7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_138656</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375898.1">
        <property type="nucleotide sequence ID" value="XM_007375836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANZ7"/>
    <dbReference type="STRING" id="619300.G3ANZ7"/>
    <dbReference type="GeneID" id="18870137"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138656"/>
    <dbReference type="eggNOG" id="ENOG502S63H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024893_2_1_1"/>
    <dbReference type="InParanoid" id="G3ANZ7"/>
    <dbReference type="OMA" id="CPGYKDQ"/>
    <dbReference type="OrthoDB" id="2537459at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="3">
        <location>
            <begin position="31"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="448" mass="47670" checksum="F4725D675932DDC4" modified="2011-11-16" version="1">MGQQQSGSLLQLRNYAWVVAILLALLPVTSGLTLNLCSTTNLAGDSSPSNSIYMSNGLCQNTCLNQGKGVAILSGTNCYCTDNVPADTVDLSKCAIGCPGYKDQENCAGNGYFGYLIINGPSSTVTKQSPTPSTAPSTNKKTQPTDNNQPSNEPSSDQPSADQPGTTMITQTTEITQTQQDSDIVKVTTTVSKVNSDSSQASMQPSTVFSYVTINGTLSQEIKTLYITQVTAETSSTDPSDSLAVSSNVNDREGAPTTTSDTDAKEKADQNKSTFFDDTAKVAGTFTAVGVVVLGLVSGILYCCCAGSRNHHNGDYTDEESQSSGDELSIDHEKQAGTQLSTPSHSVKRNSSHKSVFKLFTGEKDSVNRSSSRKKLMSKNSPNPNIPEDANASAGGSIIMFPINEVDARLDPYTMFLNTNLSKQSLGDEKDYSRKLKIINPEISAKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP29</accession>
    <name>G3AP29_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Elongation factor 1-gamma 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_61149</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375336.1">
        <property type="nucleotide sequence ID" value="XM_007375274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP29"/>
    <dbReference type="FunCoup" id="G3AP29">
        <property type="interactions" value="1289"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP29"/>
    <dbReference type="GeneID" id="18873744"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61149"/>
    <dbReference type="eggNOG" id="KOG0867">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_3_0_1"/>
    <dbReference type="InParanoid" id="G3AP29"/>
    <dbReference type="OMA" id="VQWATEN"/>
    <dbReference type="OrthoDB" id="249703at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03181">
        <property type="entry name" value="GST_C_EF1Bgamma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03044">
        <property type="entry name" value="GST_N_EF1Bgamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000006">
        <property type="entry name" value="Elongation factor 1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000142">
        <property type="entry name" value="Elongation factor 1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1010:FF:000001">
        <property type="entry name" value="Elongation factor 1-gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1010">
        <property type="entry name" value="Translation elongation factor EF1B, gamma chain, conserved domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050802">
        <property type="entry name" value="EF-GSTs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001662">
        <property type="entry name" value="EF1B_G_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036433">
        <property type="entry name" value="EF1B_G_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00647">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01183">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89942">
        <property type="entry name" value="eEF1-gamma domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50040">
        <property type="entry name" value="EF1G_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="8">
        <location>
            <begin position="85"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="EF-1-gamma C-terminal" evidence="6">
        <location>
            <begin position="251"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="216"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="216"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="413" mass="47005" checksum="5A0C0D12305B63E6" modified="2011-11-16" version="1">MSQGTLYVMEMSPRSYVNANIVKHFGLDVAIKTEKDEAFNRAFPLGKLPAFIGPKGYKLHECLAISNYLVSLVPKDEVKGFLGKNNQEFASIIKYLSLVNQEFVPAQYQALFMINGRFPYNKKQVDEHLANAEKVAAILEARLSEFTYLVGERATLADFFVAASISDGLKTIFAKPFAKKFPSIARWFQTVSKHAFFEGKFADFVIPEQALVYTPPKKEKKEKKPEAAAAPKKEAKKEVAEEAAPAEAPKPKHPLELLGKPKAVLDEWKRVYSNEETRETAIPWFWKNQYDPEEWSLWKVDYRYNDELTLTFMSNNLVGGFFNRLSASTKYMFGCMVVYGENNNNGITGAFLVRGQDYKPAFDVAPDWESYEFTKLDASNEESKNFINNMFAWDEPVIVNGEKREIADGKVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP62</accession>
    <name>G3AP62_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WD40 repeat-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_51169</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375909.1">
        <property type="nucleotide sequence ID" value="XM_007375847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP62"/>
    <dbReference type="FunCoup" id="G3AP62">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP62"/>
    <dbReference type="GeneID" id="18871528"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51169"/>
    <dbReference type="eggNOG" id="KOG0283">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011299_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP62"/>
    <dbReference type="OMA" id="FHPNDDR"/>
    <dbReference type="OrthoDB" id="1932312at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040324">
        <property type="entry name" value="WDR44/Dgr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14221">
        <property type="entry name" value="WD REPEAT DOMAIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14221:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="232"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="309"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="349"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="200"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="855"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="200"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="881" mass="99088" checksum="5F942795489D1571" modified="2011-11-16" version="1">MATGQVTAPPRSPSPTPPKRGFFNKLIKRKNVKKDGSKGLSRQSSDSNRPNKDRKLVVARSKSTNRGRNRSNSTLNRNSYVDNDEFSLYNEDELESSTIVSTDESSVESFDQSFATGVPTFQKPPQSLTFDNHLASEQVKFPNIETYFPEGWKEKDEEPPWSGITFESFINPKYVKVSRRHKQSPRVLNNLFLAQELNMEHHDPGSRSREGSVSDEELGSIDGKLSEEDDGLKCSNNEIFVMEFSKDGKYLAAAGRDSAIKIWKVISSPLGRLEYSHKMKQEGVPPQSNKRDPVFHSAPVFHQSPVRIFKGHTQNVLSLAWSKNNFLISGSMDKTVRLWHVDRPKCLQVFQHEDFVTSVKFHPLDDRFFLSGSLDNHVRLWSILEKSISYTKNLGEDVLITALEFTPDGNLCVVGGFNGSVFVMETKGLFVLKRFEVKERSLVNAFHSKTGNKITGIKVFVNPFYIEGQLPKVSEIDKWAVLITTNDSKVRMISTTSKKLITRFKGLTNNSSSIQASSSEDGKYIIAGSEDHYCYIWENNSSIINNKLKQSLADLIDRTQHATDLHTKINKIIPAHIRKLLDEDKNEFIANENNSYSCFHAHHSKCNVAIFAPENTRKILQSSDDIIYDLEKRGDRCKFESAIHEGESKTIHKDKTNDPDLRMGHIVVTTDQFGLIRVFRQDSSREYRKKFVDCYKRCQSSTKTSYPNTPIDGFNQIGAMAVRSLSPSQVLDPSKQIRDILSTGMKVTTSTLNPQNTINGIAAIPKVISSSSIMNLKQAARNRACPKPIKSHSDDVLMASKDGFYSNKNGSEVTLNAIPNQKAKVISPTEQFPTMGAEFIPSSIPVLITNTDIKPSNESLSSHQKLENLKISRSNSRGRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3APA8</accession>
    <name>G3APA8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vacuolar-sorting protein SNF8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_138693</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the endosomal sorting complex required for transport II (ESCRT-II).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the SNF8 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375361.1">
        <property type="nucleotide sequence ID" value="XM_007375299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APA8"/>
    <dbReference type="FunCoup" id="G3APA8">
        <property type="interactions" value="664"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APA8"/>
    <dbReference type="GeneID" id="18870141"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138693"/>
    <dbReference type="eggNOG" id="KOG3341">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070147_0_1_1"/>
    <dbReference type="InParanoid" id="G3APA8"/>
    <dbReference type="OMA" id="QIVEVCM"/>
    <dbReference type="OrthoDB" id="283883at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904669">
        <property type="term" value="P:ATP export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000742">
        <property type="term" value="P:karyogamy involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000397">
        <property type="entry name" value="Vacuolar-sorting protein SNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016689">
        <property type="entry name" value="ESCRT-2_cplx_Snf8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040608">
        <property type="entry name" value="Snf8/Vps36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12806">
        <property type="entry name" value="EAP30 SUBUNIT OF ELL COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12806:SF0">
        <property type="entry name" value="VACUOLAR-SORTING PROTEIN SNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04157">
        <property type="entry name" value="EAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017215">
        <property type="entry name" value="ESCRT2_Vps22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="234" mass="26390" checksum="22263F1A81053FB6" modified="2011-11-16" version="1">MNHNDNRDAYSQLGKSLIQRHSDQLSTQLAVFQSALVNFANSHGDEIKSNAEFRNKFTQISQSIGLDPLELLLSTTKNTKSSNFHVALAVRIVEVCQSTRDLNGGLISLKELKSILQDTHNISIEVSEGDIEQALGMLDTLGKGYQVLTINDKKWLKFTSANGDGISQDEKKVYELCGFMGGYVTYRLLRDNYGWDKVRCKTVIEEMIMNGFLWVDNQGSKGELQFWEPSWISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3APM5</accession>
    <name>G3APM5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Derlin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_139495</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in the degradation of misfolded endoplasmic reticulum (ER) luminal proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the derlin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375472.1">
        <property type="nucleotide sequence ID" value="XM_007375410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APM5"/>
    <dbReference type="STRING" id="619300.G3APM5"/>
    <dbReference type="GeneID" id="18870212"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139495"/>
    <dbReference type="eggNOG" id="KOG0858">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_891365_0_0_1"/>
    <dbReference type="InParanoid" id="G3APM5"/>
    <dbReference type="OMA" id="LPPWYWV"/>
    <dbReference type="OrthoDB" id="1716531at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007599">
        <property type="entry name" value="DER1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11009">
        <property type="entry name" value="DER1-LIKE PROTEIN, DERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04511">
        <property type="entry name" value="DER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="288" mass="34086" checksum="AC77ED431C877ADA" modified="2011-11-16" version="1">MDALWTSLPPVTRGWNIAIVATSTLVTLKKVKLSSLLFQPERAFTNQIWRLVSSFFAFGDLSMNLFMEIWFVSSSTRRVEDAFITNSSMLPQVVDSFDAEQRKLLHDFIERNKTIDFLYYLLQICIAIVLSATFGYYKLGITLPQLGKLLCHSLIYIDSRITPEEVLNLFGIFQFKKVYYPWVCASLDLLVVAVNLLDINWKWYVEVILKPFIWFYTVSYGLGHLWWVLRDFLLQEVHNDQNDKRRLLRQNTLREFKVTRFDLVKEVLSMVFLPPWYWVVISKIQRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3APT2</accession>
    <name>G3APT2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_138497</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375529.1">
        <property type="nucleotide sequence ID" value="XM_007375467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APT2"/>
    <dbReference type="STRING" id="619300.G3APT2"/>
    <dbReference type="GeneID" id="18870124"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138497"/>
    <dbReference type="eggNOG" id="KOG1502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_9_2_1"/>
    <dbReference type="InParanoid" id="G3APT2"/>
    <dbReference type="OMA" id="KNEECWA"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05227">
        <property type="entry name" value="AR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="334" mass="37326" checksum="439EB3D011F72FCD" modified="2011-11-16" version="1">MTSTSVFVSGANGFIAQHVVKELLSKGYTVIGSVRSEAKGEELKSLVKNDNFSYEIVASLTEEGAFDEVLKKHPEVTVFLHTASPFSFSVEDVEKDLMKPAINGTLNALNAIKKYGPQIKKVVITASAVANFGFGPHFDAEKVYNEDDWNPITYKESLENSFFGYFGSKKYAELAVHDFMAKETVNFDISFINPPYVFGPQAYVVKDKANLNLSNEVINRVVKLSKDDTIPAEGSVFVDVRDVARAHLVAFENDETVNKRLLTIAGRYTLDTIANIINRYFPSTTVPKGDESRNDEIYKNVHKYDNSRTNKLLGFEYTSLADTIFDTVEQIYNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3APZ5</accession>
    <name>G3APZ5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">GPI ethanolamine phosphate transferase 1</fullName>
            <ecNumber evidence="14">2.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_55800</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 14">Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the first alpha-1,4-linked mannose of the glycosylphosphatidylinositol precursor of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the PIGG/PIGN/PIGO family. PIGN subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.-.-.-" evidence="14"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375592.1">
        <property type="nucleotide sequence ID" value="XM_007375530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APZ5"/>
    <dbReference type="FunCoup" id="G3APZ5">
        <property type="interactions" value="497"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APZ5"/>
    <dbReference type="GeneID" id="18871973"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55800"/>
    <dbReference type="eggNOG" id="KOG2124">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007676_0_0_1"/>
    <dbReference type="InParanoid" id="G3APZ5"/>
    <dbReference type="OMA" id="QSYFHRE"/>
    <dbReference type="OrthoDB" id="2748310at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015867">
        <property type="term" value="P:ATP transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16020">
        <property type="entry name" value="GPI_EPT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000015">
        <property type="entry name" value="GPI ethanolamine phosphate transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007070">
        <property type="entry name" value="GPI_EtnP_transferase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017852">
        <property type="entry name" value="GPI_EtnP_transferase_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037671">
        <property type="entry name" value="PIGN_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250:SF0">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN, CLASS N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04987">
        <property type="entry name" value="PigN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 14" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="477"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="511"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="537"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="566"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="593"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="650"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="683"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="713"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="744"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="807"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="853"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="888"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="920"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="domain" description="GPI ethanolamine phosphate transferase 1 C-terminal" evidence="15">
        <location>
            <begin position="461"/>
            <end position="912"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW32316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="959" mass="109368" checksum="5C11A1FB8A7BCBA0" modified="2011-11-16" version="1">MNKNRQLLVFIGVLFHFLYLWSIFDIYFVSPLVHGMNHHLSTETPPAKRLFLIVGDGLRADKTFQKLTHPKTGEDKYLAPYLRGIVQNNGTWGISKTRMPTESRPGHVAMIAGFYEDVSAVTKGWKENPVNFDSFFNQSKHTYSFGSPDILPMFAYGDGVVPGRIDVCMYGHEFEDFTQSSIELDSFVFKHFDDLLANSTVNQTLHDELHQDGNVFFLHLLGCDTAGHAYRPYSAEYYDNIEYIDTKLAELVPKINEFFGDDQTAFIFTADHGMSDFGSHGDGHPDNTRTPLIAWGAGINKPKKLTDLPESQAVIQDPINSGFEKDYFNTWDFDHLGRFDVNQADIASLMAYLIGAQYPANSVGELPLGYLDATQSTKIKALYANALAIVEQYIVKQDEVQDHQFKFKPFNPFEEKPITNYTSEIEKLIETLNQDLAQDLKIEIEHQAISLAEELMKKALEGLTYLQTYNWALLRSIVTLGFFGWILYSFNIFLKLFILNESDLEKYKSKSSYILIGIFVAISASINYLLYYQNSPINYYMYTWFPLYFWYTILDEQEYFKQGVDIFLHGISSFTRIVLVVIFIGMYEAIAYGFFQRFIFSVIFVLVGLYPLAVNGSNLINNKLKISWFASCFFMCFFTNSNPVKVESLTQINIGGILSFVVAIMGAKKVFVNKEVDYIQRKLIWYQILAIPIMLYATNVSVISLQARDGLPLLSQILGWITFIVSILVLPILHSTAPSTDYELRLLIIFLTFTPTFIILAISFEVLFYVGFSLVLLQWLTIEESLKIPHESIVKSQKETGRLPEGYWLQVIRIAIIGFFFLQIAFFGIGNVASISSFSLDSVYRLIPIFDPFSMGALLMLKLIIPYVLLSTCLGIMNHQLEIEKFTISSLIISTSDFLSLNFFFLVRTEGSWLDIGVSISNYCLAILSSLFMLILELFSSLVLKGVKYERVDVSKKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ69</accession>
    <name>G3AQ69_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 16">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_61980</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.</text>
    </comment>
    <comment type="function">
        <text evidence="16">GTP-binding protein involved in protein trafficking; modulates vesicle budding and uncoating within the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31416.1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="10 16" id="KW-0449">Lipoprotein</keyword>
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    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 16" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 13 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 16" id="KW-0653">Protein transport</keyword>
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        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
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    <feature type="binding site" evidence="14">
        <location>
            <position position="31"/>
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        <ligand>
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        <location>
            <position position="48"/>
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        <ligand>
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        </ligand>
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        <location>
            <position position="70"/>
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        <ligand>
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        <location>
            <begin position="126"/>
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    </evidence>
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        </source>
    </evidence>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="181" mass="20602" checksum="FFC016DF13098652" modified="2011-11-16" version="1">MGLSFSKLFANLFGNREMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRYYFQNTQGIIFVVDSNDRDRIAEAREELQQMLNEDELRDALLLVFANKQDLPNAMNAAEITEKLGLHSIRQRPWYIQATCATTGDGLYEGLEWLSTNLKNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ83</accession>
    <name>G3AQ83_SPAPN</name>
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            <fullName evidence="2">Glycosylphosphatidylinositol synthesis N-acetylglucosaminyltransferase complex, subunit PIG-Q/GPI1</fullName>
        </submittedName>
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    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_154575</name>
    </gene>
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        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT Q"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="493"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="530"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="646" mass="76537" checksum="07FDC111A49E9646" modified="2011-11-16" version="1">MVSTDSPVQIYFSNDVRKYLNHFKDSEKDELLLLGYKFNHIYIVFLPVKQIDLEKLPQELRDLQIIGTFSNTNKQANHAYDLNIKFDPRTDKIPKIISPSSSYVVFFDPPNFKNLEYFSITPILLESMKSDEEATTSGKIDYIALLEKWEPELHIDDNQFFISDDQILDKINHLYRNRLVFQKWWLAESGTHFLPSLFNSEQLNVIRPIKSAFTSLLIPITQFIQKLLITIIKIINFKFGKFSLVTLSKIFRQLDLRLQQFNYFPIQFLCYYNKNLLYNEQNGKSSSVIDRLKLPIFNSNLNINNSNYINLYNSIWLIFNDVLIGQAVYGLIEENFDDIVELIRRSIQRYLFQDMMELITWVSSKHPAGFKLNNELGKFMGDLYLWTLWFWKLVEENFQLSEPTRIKLFIKIICCFGGVSFLVSFLIDVMNVATYHIYTFYYCSTKIYKRQLEVIKSLFQLFRGKKYNVLRNRIDNLSTTQVNSSGFDFIDQLLLGTLIFMILILLLPTVFAFYLMFFLMHLVSIMTVNLLENIQILINFTPLFVILLKLKNSKRLQGGIKFEFLKYIPNTTYIKMKNKALTNGEIFTNFFKLFKRTKNFRNSIIYNFLSGELIVIKYDYLLKFHYLMLPDVYSETINVWKYLPRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQA8</accession>
    <name>G3AQA8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1749-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62026</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376233.1">
        <property type="nucleotide sequence ID" value="XM_007376171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQA8"/>
    <dbReference type="GeneID" id="18874155"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62026"/>
    <dbReference type="eggNOG" id="KOG4840">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049633_3_0_1"/>
    <dbReference type="InParanoid" id="G3AQA8"/>
    <dbReference type="OMA" id="PPWVNKE"/>
    <dbReference type="OrthoDB" id="10034502at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013744">
        <property type="entry name" value="SidJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31591">
        <property type="entry name" value="UPF0613 PROTEIN PB24D3.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31591:SF1">
        <property type="entry name" value="UPF0613 PROTEIN PB24D3.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08538">
        <property type="entry name" value="DUF1749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="308" mass="34390" checksum="7F898556C3C6C2B4" modified="2011-11-16" version="1">MSLSSVTPQYGIVHTYGFNLTAFEFTSEGKLASNILLFVGGLGNGLLNVPYLPELSHAASTQFKSLDGESWSLVQVLLSSAYSGWGTSSLERDVRQLEQAIKYFRSDKGGNRKKVVLMGHSTGCQDTIKYLTQSKHSKHGEIQGGILQAPVSDREAFRHGRDVTEFEKLVQNVYDNYISQGREKELLPEKYRKISFNTPITAYRFYSLASERGDDDYFSSYLTAEDYKESFGKVDKPILVLYSGNDQFVPAYVDKQKLIDTWKSATADQYWSPYSKVLEGGLHDLGDGSTNNAIPDLVESVTSFISTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQB4</accession>
    <name>G3AQB4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Sterol 24-C-methyltransferase</fullName>
            <ecNumber evidence="2 8">2.1.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Delta(24)-sterol C-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_62031</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of zymosterol to form fecosterol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>zymosterol + S-adenosyl-L-methionine = fecosterol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17038"/>
            <dbReference type="ChEBI" id="CHEBI:18252"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Steroid metabolism; ergosterol biosynthesis; ergosterol from zymosterol: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7 8">Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6/SMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.41" evidence="2 8"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376238.1">
        <property type="nucleotide sequence ID" value="XM_007376176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQB4"/>
    <dbReference type="FunCoup" id="G3AQB4">
        <property type="interactions" value="327"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQB4"/>
    <dbReference type="GeneID" id="18874157"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62031"/>
    <dbReference type="eggNOG" id="KOG1269">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039068_5_0_1"/>
    <dbReference type="InParanoid" id="G3AQB4"/>
    <dbReference type="OMA" id="NYHEIYG"/>
    <dbReference type="UniPathway" id="UPA00768">
        <property type="reaction ID" value="UER00760"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003838">
        <property type="term" value="F:sterol 24-C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050447">
        <property type="entry name" value="Erg6_SMT_methyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030384">
        <property type="entry name" value="MeTrfase_SMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013705">
        <property type="entry name" value="Sterol_MeTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44068:SF1">
        <property type="entry name" value="HYPOTHETICAL LOC100005854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44068">
        <property type="entry name" value="ZGC:194242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08498">
        <property type="entry name" value="Sterol_MT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51685">
        <property type="entry name" value="SAM_MT_ERG6_SMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3 7 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="8" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="8" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="8" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase Erg6/SMT-type" evidence="9">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="259" mass="29383" checksum="0F8AED7ADC0469DB" modified="2011-11-16" version="1">MNLQENMKVLDVGCGVGGPGREITRFTDCEIVGLNNNDYQIERANHYAKKYKLDHKLSYVKGDFMQMDFEPETFDAVYAIEATVHAPVLEGVYSEIYRVLKPGGVFGVYEWVMTDEYDENNEEHRKIAYGIEVGDGIPKMYKREVAEQALKNVGFEVEYERDLADVNDEIPWYYPLAGELQNCQTFGDYWTVLRTSKVGRLVLTELVGLMEKVGLAPQGSKQVTHALEDAAVNLVEGGRKKLFTPMMLFIARKPETASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQK8</accession>
    <name>G3AQK8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cleavage and polyadenylation specificity factor subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Cleavage and polyadenylation specificity factor 100 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_67601</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF2/YSH1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376333.1">
        <property type="nucleotide sequence ID" value="XM_007376271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQK8"/>
    <dbReference type="FunCoup" id="G3AQK8">
        <property type="interactions" value="836"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQK8"/>
    <dbReference type="GeneID" id="18875173"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67601"/>
    <dbReference type="eggNOG" id="KOG1135">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002227_3_0_1"/>
    <dbReference type="InParanoid" id="G3AQK8"/>
    <dbReference type="OMA" id="FWLIVKR"/>
    <dbReference type="OrthoDB" id="64353at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16293">
        <property type="entry name" value="CPSF2-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd24141">
        <property type="entry name" value="NDUFS5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027075">
        <property type="entry name" value="CPSF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025069">
        <property type="entry name" value="Cpsf2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035639">
        <property type="entry name" value="CPSF2_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45922">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45922:SF1">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13299">
        <property type="entry name" value="CPSF100_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Beta-Casp" evidence="6">
        <location>
            <begin position="250"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="455"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="586"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="461"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="586"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU365006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1032" mass="116183" checksum="52B41E419026F617" modified="2011-11-16" version="1">MFSFSLISPSEGDLSFKASLLSFDNEFKLLADPSWDGKDANAVLFMEQHLSEVNAVLLSHSTPDFISGYVLLCLKFPNLMSTMPVYSTLPVNQLGRISTVEYYRANGVLGPLDSAILEIDEVDNWFDRVTLLKYQQSTNLMDNKVTITPYNAGHTLGGAFWLIVKRIDKVIYAPAWNHSKDSFLNSASFISTSTGNPLLSLLRPTAFITAPDLGSTMPHKRRTEKFLQLVDATLANGGAALLPTSLSGRFLELFHLIDEHLQGAPIPVYFLSYSGTRILSYASNLLDWMSGSFIKSWDETSGDGGRGGGKALSSMPFDPSKVDLLLDPSELIQLSGPKIVFCSGIDIKSGDISSETFQYLCNNEKTTVILTEKSQLENGGLNSMLYKEWYELTKKKLGGKIEDGTAVPLDKTVSIEDWTRETNLEGRELSDFQERITQQRKEKLLAKVRDKKNQNILNAENVDDEDSSEDEDEEEQVPEEETKGEAAKSVTTTDAIVTTATSVVDELAAHEAFVMDHIKQSLKDNIPIDLKITHRLRPRSAMFPYFMTTRKQKFDDYGQVIDVADFEKTDETSNAKIIMEGKKKFDEKRRWNEEKSNDDNKQKKNQNKQQANKLTPQEQLNQQLIQKNLDTLYNPRKRVPLNAASSFASQSQMLKIRCGLSFVDLSGLVDLRSLGIIVQALKPYNLLLLPDFNSIPELEVNGLKQVQEMFDHQQNEQLQEESKKKILTSSRYLSLSSIRSGLSSAISTYGGNKMNVLVVESDKPVKIGTESESGGIGLNNFEINLDDSIIKDLRWQKIGSNYRVAKVHGELELQNPIAKEKTERTLSDYINPTTYFTLKKSDDESLRKRRKVVNQRLQDPKLKPLISNGPKLAIGNIRLPDLRKNLQEKNMNAEFKQEGTLVVNDVLTIRKVAYGTSESDDSGDIVIEGNYSRLHQYTKMRPYSKSGGTAKCFYEFQTFVACYTSAETVNRKECTPAFEDYAECLHGTKERERARLMMQQLKENEQNKSGVTASDLYKSSGRVYENLDLIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQT4</accession>
    <name>G3AQT4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_62242</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376409.1">
        <property type="nucleotide sequence ID" value="XM_007376347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQT4"/>
    <dbReference type="GeneID" id="18874229"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62242"/>
    <dbReference type="eggNOG" id="ENOG502RMT1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135158_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQT4"/>
    <dbReference type="OMA" id="NFADHYQ"/>
    <dbReference type="OrthoDB" id="4019199at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442515" evidence="2">
        <location>
            <begin position="21"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="132" mass="14955" checksum="76396289543D3FEC" modified="2011-11-16" version="1">MVFMIPLSVKIALTVGAVVGASVAVIHNKEAILETAEHLFEQGAEFCRNKLNEAKQMNEMHFADAYEDFSFSRDEEDEKHETKGRSTGSRLTNESDYDEISTPETSDFSEFSEMSEFSEIEDDDRLENDSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQW1</accession>
    <name>G3AQW1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4484 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_61761</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375968.1">
        <property type="nucleotide sequence ID" value="XM_007375906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQW1"/>
    <dbReference type="FunCoup" id="G3AQW1">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQW1"/>
    <dbReference type="GeneID" id="18874037"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61761"/>
    <dbReference type="eggNOG" id="KOG4704">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_910773_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQW1"/>
    <dbReference type="OMA" id="ELAQCHA"/>
    <dbReference type="OrthoDB" id="2152680at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018626">
        <property type="entry name" value="LCHN/Anr2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053056">
        <property type="entry name" value="Lipid_Metab_Assoc_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28153:SF1">
        <property type="entry name" value="DUF4484 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28153">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09804">
        <property type="entry name" value="DENND11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="306" mass="35212" checksum="6EC7E61654753EE3" modified="2011-11-16" version="1" fragment="single">MSYTPSNVVSIFLTEFHPRNGYKLVWSRSTIPDFDFTGLDYKSMPSGVHEYGKSTVLISHTSQDKLYYGICKFRQHLIGEDTHDRNNIKIYSLGIICQPSESLFKPNEFINNGWEFIGDLDHELLKYLQEQKYEDFAVFEKLFESLCKPSSNLLPLPNAKPVDVTNHPLTKLPQLFKTFGPLVFVLYKQALLRKRILIFNQHHIFHDDEDLLPNEDSDLLLNLSTFCYLVSLVSIIPQDIEIASQTYSQPIYSIGLNDLTGDLVKIDNYIGTTNDDILIENKVYDIGVMIDDKVTIFPMDDKNNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQW4</accession>
    <name>G3AQW4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">enoyl-[acyl-carrier-protein] reductase</fullName>
            <ecNumber evidence="11">1.3.1.104</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_61769</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 2,3-saturated acyl-[ACP] + NADP(+) = a (2E)-enoyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22564"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9926"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78785"/>
            <dbReference type="EC" id="1.3.1.104"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.104" evidence="11"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375971.1">
        <property type="nucleotide sequence ID" value="XM_007375909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQW4"/>
    <dbReference type="STRING" id="619300.G3AQW4"/>
    <dbReference type="GeneID" id="18874039"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61769"/>
    <dbReference type="eggNOG" id="KOG0025">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_17_1_1"/>
    <dbReference type="InParanoid" id="G3AQW4"/>
    <dbReference type="OMA" id="GQYATQI"/>
    <dbReference type="OrthoDB" id="7482721at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141148">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08290">
        <property type="entry name" value="ETR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051034">
        <property type="entry name" value="Mito_Enoyl-ACP_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43981">
        <property type="entry name" value="ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43981:SF2">
        <property type="entry name" value="ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="13">
        <location>
            <begin position="32"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW31193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="265" mass="28737" checksum="A05A02EE312CD2B3" modified="2011-11-16" version="1" fragment="single">MFSSLVRTTRTPVLTQLRTMITAQAVLYKQHGEPKDVLFTQSFQIDDENLSGNQVVVETLASPVNPSDINQIQGVYPSKPEKTTQFGTDEPAAPCGNEGLFQVIAVGDNVEDLKVGDWVIPANVNFGTWRTHALGNDSDFIAIPNPAQSKENGKPLGLSINQGATISVNPLTALLMLTHYVKLTPGKDWFIQNGGTSAVGQYATQIGQLLDFNSISVIRDRPNLEETIADLKSKGATQVITEDQNGSKEFGPVIKNWVKETGGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR38</accession>
    <name>G3AR38_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_56494</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376477.1">
        <property type="nucleotide sequence ID" value="XM_007376415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR38"/>
    <dbReference type="GeneID" id="18872039"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56494"/>
    <dbReference type="eggNOG" id="ENOG502RPSC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_370523_0_0_1"/>
    <dbReference type="InParanoid" id="G3AR38"/>
    <dbReference type="OMA" id="PFIMFHY"/>
    <dbReference type="OrthoDB" id="4075894at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="751" mass="86518" checksum="A9475F5E2D1F284F" modified="2011-11-16" version="1">MNKSIIRRLRSNVTIKRLSVSYRELATAARPTQARPIIPTAFKPSQPWETELLGLPNDHPHNPLIDFSKDEIVNLTQKEYRHLNGLSYGTLEEMSGFCLNIIEIVQEKITEDPRLVSWLIREINDTTLKETITSALAKSFQDDEVKSCIIQFIIDPTSTAFRVKFFQVFMSILELTKGDKAHKGHILLNFLEKLSRTNVINSMPLLLSGQQYHQILEIVPREKRAALFSRMVCVNLQTIATNKMERFKKILYHGTSIEKLISRTGLLFPKWHDLHKTDFGEHNERIIEFYTVKDLSTYCNMFIDSEDPANAILYLKFLLEKYERKYSRGAFLKGDTSLHEDTQSLLLVILNFVTKFKGVSHSAQVLKYLTEEGLTVDYDTYLLLMRNMRLQGYYNEAIVVMNNIDLNGISNSFKKKWAKEIMLLMRAKFPSSPKVIIGYAGALYGDEAHRVLALFNDLKILPIVYSNGSLEPLHSFEEVKLASVDKSLTGNKFTTEVLASIYNVLLSSIPLGTTLPRLINKLFEEYMRVIQTPETTLDANDDVIRVFLKHLLKDTSSTELQVSTEYENFAVAKRIFTYYERVPLPYGEINPVNIDLLIQAALLVHKDYEFATRVMKLYRKKQSNLTFPQIYSFVKYHHEKSEYDLAKHWYDELVRQGAQYVGPLYRSLFDIARKMDWPVTGHAYKWGITKKHRRDRQAMATIHSGSLSFLGKDFPQGDLKSGQSEPNAVNFGSELAATLHEIKKLNKRTCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR59</accession>
    <name>G3AR59_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein PTR2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PTR2</name>
        <name evidence="10" type="ORF">SPAPADRAFT_56124</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376012.1">
        <property type="nucleotide sequence ID" value="XM_007375950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR59"/>
    <dbReference type="FunCoup" id="G3AR59">
        <property type="interactions" value="1115"/>
    </dbReference>
    <dbReference type="GeneID" id="18872000"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56124"/>
    <dbReference type="eggNOG" id="KOG1237">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004790_4_2_1"/>
    <dbReference type="InParanoid" id="G3AR59"/>
    <dbReference type="OMA" id="QMMGVWF"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071916">
        <property type="term" value="F:dipeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000085">
        <property type="entry name" value="MFS peptide transporter Ptr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01023">
        <property type="entry name" value="PTR2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="361"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="450"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="480"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="509"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="570" mass="64400" checksum="155B757DF092F7C7" modified="2011-11-16" version="1">MSVEKVPEESLDKNLSNVEQNVSLGGDFENDDGREPTDLELKTLRHISESIPFRCWLVAIVELAERFAYYGLSAPFQNYMQNGPNDTPPGLLALNQSGASALSYFFQFWCYVTPILGGWIADTYWGKYKTIFYFCILYIIGIFILFITSLPSITSRTTGLAGYIVSIIIIGLATGGVKSNVSPLIADQIPKTKPVIKVLKSGERVIQDPNITIQNVFMFFYLMINIGSMSVIATTEMEAHIGFWAAYLLPFCFFFIALAALILGRNQYVKIPVSEKIINKTFKCAWIAVTNKFDLEECKPSVHPERNYPWTDHFVEEIRRALYACKVFIFYPIYWVVYGQMLNNFVSVAGTMELHGLPNDILQAIDSLTIIIFIPIFERLVYPFIRRFTPFKAVTKIFWGFMFGTAAMVYAAVLQHYIYKAGPCYDMPMACAPEYANTPNHIHVALQTPAYFFIAISEILASITGLEYAYTKAPISMKSFIMSIFLVTNAFGSILGIAISSTFEDPKMVWTFTGLAISCFIAGVAFWLCFKHYNLKEEELNNLEYDGDDEHIPHDLQPITSLAHSHKSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARU8</accession>
    <name>G3ARU8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_56223</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376143.1">
        <property type="nucleotide sequence ID" value="XM_007376081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARU8"/>
    <dbReference type="GeneID" id="18872018"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56223"/>
    <dbReference type="eggNOG" id="ENOG502QWM5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034486_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARU8"/>
    <dbReference type="OMA" id="SWRMTTP"/>
    <dbReference type="OrthoDB" id="3364892at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF26">
        <property type="entry name" value="CARRIER PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G07710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 12" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="162"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="420" mass="48146" checksum="C21A5B409E17A1BE" modified="2011-11-16" version="1">MKEAPNIKDGKPRSITAPNLISIFSGAPIYNDSVIFDDQSHQTVSLSAAGIRAIIYQLQGLYLRTPVKLFRPSRFDYLAQVRVLANKHHNLSEKPYRFRTHSSLAMLFQVIRKEGWKFIPDHVLPPLIANSATGLILYATYLTTLDHYNKKHQTPPAKFNWYSPFDTWRAGFIAGAVQSLAAAPVDAIYTRSTVSEMLEGTHRNLWVFGLHKLKEIGLVGVFAGYGFSLIKESFGFAFYFSTFETVKTEGYDLTHRTIMWYHRSEEIIKRKLQKIFKWNEEQVDEKMEKLERYRSERLLKSTFVLLAGASAACSLLAVQYPLSKIQKLHLSRLEALDVYSASKHSTKPFITLYYNSYIDTFNQVLRMKRKSGVTWIEAGYKGFVRNALTTIPATSVGLLVFEMMRTKLADNFEEYELLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARW6</accession>
    <name>G3ARW6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Histone H3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_141430</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376593.1">
        <property type="nucleotide sequence ID" value="XM_007376531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARW6"/>
    <dbReference type="SMR" id="G3ARW6"/>
    <dbReference type="STRING" id="619300.G3ARW6"/>
    <dbReference type="GeneID" id="18870374"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141430"/>
    <dbReference type="eggNOG" id="KOG1745">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078295_4_0_1"/>
    <dbReference type="InParanoid" id="G3ARW6"/>
    <dbReference type="OMA" id="THRFKPG"/>
    <dbReference type="OrthoDB" id="5326060at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000010">
        <property type="entry name" value="Histone H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00322">
        <property type="entry name" value="HISTONE_H3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="10 11" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="13">
        <location>
            <begin position="44"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW31815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="136" mass="15356" checksum="B2D84C8974FFBD3A" modified="2011-11-16" version="1">MARTKQTARKSTGGKAPRKQLASKAARKSAPVSGGVKKPHRYKPGTVALREIRRFQKSTELLIRKLPFQRLVREIAQDFKSDLRFQSSAIGALQEAVEAYLVSLFEDTNLCAIHAKRVTIQKKDIQLARRLRGERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARW8</accession>
    <name>G3ARW8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Septation initiation network scaffold protein cdc11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_67833</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376595.1">
        <property type="nucleotide sequence ID" value="XM_007376533.1"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARW8"/>
    <dbReference type="GeneID" id="18875187"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67833"/>
    <dbReference type="eggNOG" id="KOG0531">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_270310_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARW8"/>
    <dbReference type="OMA" id="NTILHNS"/>
    <dbReference type="OrthoDB" id="7451790at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061499">
        <property type="term" value="C:outer plaque of mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902412">
        <property type="term" value="P:regulation of mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031028">
        <property type="term" value="P:septation initiation signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052574">
        <property type="entry name" value="CDIRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47566">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47566:SF1">
        <property type="entry name" value="PROTEIN NUD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="200"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="353"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="426"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="541"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="760"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="27"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="85"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="360"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="426"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="549"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="760"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="772"/>
            <end position="789"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1319" mass="148539" checksum="0890B28DE70F5F6A" modified="2011-11-16" version="1">MTSTLRNNTPLSTVVNGDPLKLDSDQENQPPSSDDNVNTIMNEKKFIQQSNVPEPINTNSMLPKPKKAASISNLRPIDEDHELSRQQLSPPQPLLQVPSSSHYSTIPTLSEKPPSPPPVVTADRAAVTSSSQSTFTLKDINLQPEEEQSLPESSQDLSILEQSSPRHSPIKKSAYQAKLRYHLNQDFSANDKSSLFLPPGEYGPTTTKSPPPSIPNTFKYKSHPNAKTKTKIGAEILEEEEEDQHKSTGTADWMPSVLGDRWISETSHMQPTPLSTSNSDPIPSVNSNEFNSSVRITKSQPILLKQPSFATTMIHNSKIDTHETAEWKRAAEEYINKQHQNNFANIFQSIDSTGQKQPVTTTTTTAKSTHSSTLSTPIATLSRQMIPEQQQQQQQEHVEQSPLKLFRKNYDTFTKKALHGLLENLSNKSNVNTPTSGDSKNVQENRPHTVEAVPPPALNIESVRPNSEQHNIPPNIKDFTKDGHYNEAQYMQNANNVFSNLKKGYRASSYNIGQGKSHTTATSTPLTNNVRNIEEEINEYSSFSSGFNSKEDDEHERVPDELGETVHEDVDLYTQESTRSNSTQFENDSQYTFDEDYDDNGTITERTQRLSSPHRIQVNNHGFNNAPSVHYMNLEAENAALKQRIENMTKLQPSQLQPTQQSQHQPQHHEVSIVSDDNDDDNGLVIPKWKNISQLHLEPSPKHSKSQVIKGHVVPDRNLPKEYGNMIFDEKTMKWQEKKNSVHYTLDSIDDLTATQNLTSQPEISSQLHSSPIKKHMSKKTPTKKKRSTNKLEVSFHLPNADLSNAKSSQNASYDVTRMSQLEDMTFTQSHKQLISIINEVLEGEEHWERIKQITLSHFDLENTKDLDQFLPGLQRLDISNNQIQYLSGIPSRILDLDLSNNNISDITSFRDLSDLSMVDLSQNSLVRLNNLSRNIHLSELHLANNSIVSLDGLQELVKLTILDVSGNKLSGKLDFSQFYWPNLQVLNISDNQITSLVGWEEVTDLNVLLANDNQLTNFVAARPNYSLKKLGLKFNNLKAIDIDKLFDLRTLRIDGNQLEHISSLKNLHNIEDISAKSQSPNVLSTIVASTPRVSNLDLSGNGYYFNSPIFQPTPLGAVTKLTLSALGLEVIPSTFATLFPSVIDLNLNFNKLNDISHLASLKLRKLYLVGNELPRKPSISDSLVCTLYNSLRKSRSSLISLDLRMNPLTHDIYPYVISPDELDAPIQIENVEDIDGFLVHYETIKKTSDFVRRDEEFLIKNQTNIVVRRRAEYEMFCVGMFPGMSKLDGGPLTEPKRGELLKSAMVEFARRKQLKGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS88</accession>
    <name>G3AS88_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">W2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_62943</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377079.1">
        <property type="nucleotide sequence ID" value="XM_007377017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS88"/>
    <dbReference type="FunCoup" id="G3AS88">
        <property type="interactions" value="1243"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AS88"/>
    <dbReference type="GeneID" id="18874531"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62943"/>
    <dbReference type="eggNOG" id="KOG2767">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026663_1_0_1"/>
    <dbReference type="InParanoid" id="G3AS88"/>
    <dbReference type="OMA" id="MLNLKCA"/>
    <dbReference type="OrthoDB" id="10250831at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043614">
        <property type="term" value="C:multi-eIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071074">
        <property type="term" value="F:eukaryotic initiation factor eIF2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045947">
        <property type="term" value="P:negative regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.350:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003307">
        <property type="entry name" value="W2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF7">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51363">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="W2" evidence="6">
        <location>
            <begin position="234"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="333" mass="36673" checksum="95D8004BFAA98946" modified="2011-11-16" version="1" fragment="single">MSFINICRDNTDPFYRYKMPVLEAKTEGRGNGIKTAILNLAEVARALNRPPTYILKFFGYELGAQTNKVSAGDSRFLVNGAHDVNELQDTLDGFINKFVLCGSCKNPETEIVLKGKDTLERDCKACGKITMIDPKHKLYSYIVKNPPEKKGKKSATATANVVGGGKSISDIAAGQTTQSQEDVSGTQDDDDDLLAKKINAEAAQLQAVEVKDDDWAVDMSEEAIAARARELEGLTLAENKFNEFGEWLLKEAPTSKDDLPSDVEIYKKIVELDIGSSHETAQVLAQVLFDEDIIEQIEPHLGLLSKLIGNEQDFEKAFLGGLERFIGLEKPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASP1</accession>
    <name>G3ASP1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Metacaspase-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_62586</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in cell death (apoptosis).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C14B family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376760.1">
        <property type="nucleotide sequence ID" value="XM_007376698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASP1"/>
    <dbReference type="FunCoup" id="G3ASP1">
        <property type="interactions" value="371"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASP1"/>
    <dbReference type="GeneID" id="18874389"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62586"/>
    <dbReference type="eggNOG" id="KOG1546">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029389_0_2_1"/>
    <dbReference type="InParanoid" id="G3ASP1"/>
    <dbReference type="OMA" id="EYGHHTP"/>
    <dbReference type="OrthoDB" id="3223806at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050452">
        <property type="entry name" value="Metacaspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48104:SF30">
        <property type="entry name" value="METACASPASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48104">
        <property type="entry name" value="METACASPASE-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase C14 caspase" evidence="9">
        <location>
            <begin position="145"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="62"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="440" mass="48336" checksum="F820551082623BE5" modified="2011-11-16" version="1">MFPGSGHHSYNNYGPPPNQYSYPPPQGAPPQGYYAPPQGPPPQGYYAPPQGAPPQQSNYQRPPGPPPSDSYQRPPGPPPSNSYQRPPGPPPSNSYSRPEGPPPGDYGRQPYQPPQDSSFQRPPTAPQSFGQNTDFTFQYSNCSGRKKALLVGVNYFGSRQELKGCINDVKNMSKFLVDRWGYKWEDIVILTDDQREMARVPTKANIIRAMQWLVKDARPNDSLVFHYSGHGGVTEDQDGDEESGMDDVIYPVDFEQAGHIVDDDMHAIMVRPLPAGCRLTALYDSCHSGTALDLPYVYSTKGVVKEPNLLKDAGSGALNAILQYESGNLFGAISSISNVVKKVSRSGNTDSEQIKKMKASPADVISISGCKDDQTSADARENGTATGAMSWSFITVLTKYPNQSYLSLLNNMRTLLSEKYSQKPQLSSSHPQDMNIQFIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASR2</accession>
    <name>G3ASR2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Biogenesis of lysosome-related organelles complex 1 subunit SNN1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_156849</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377159.1">
        <property type="nucleotide sequence ID" value="XM_007377097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASR2"/>
    <dbReference type="FunCoup" id="G3ASR2">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="GeneID" id="18871060"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_156849"/>
    <dbReference type="eggNOG" id="ENOG502RJGT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160155_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASR2"/>
    <dbReference type="OMA" id="ESIEMRV"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="12152" checksum="4711AD10C24EFD97" modified="2011-11-16" version="1">MSVEGLHAIELAAHTLLSGSLDNLNENFDSLNQSQVIILTRLKIIEKRLSDYKQILEDNNINERELSDRFTKIKELRKRLDVCIKTLERVEQRVDRMNTKLDSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATA0</accession>
    <name>G3ATA0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Ubiquitin-activating enzyme E1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_142051</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2 11">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376896.1">
        <property type="nucleotide sequence ID" value="XM_007376834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATA0"/>
    <dbReference type="FunCoup" id="G3ATA0">
        <property type="interactions" value="1949"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATA0"/>
    <dbReference type="GeneID" id="18870415"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142051"/>
    <dbReference type="eggNOG" id="KOG2013">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013325_7_3_1"/>
    <dbReference type="InParanoid" id="G3ATA0"/>
    <dbReference type="OMA" id="PGKTECF"/>
    <dbReference type="OrthoDB" id="10255449at2759"/>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.80:FF:000004">
        <property type="entry name" value="Ubiquitin-activating enzyme E1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.520">
        <property type="entry name" value="Ubiquitin activating enzymes (Uba3). Chain: B, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.80">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.20">
        <property type="entry name" value="Ubiquitin-like 2 activating enzyme e1b. Chain: B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042449">
        <property type="entry name" value="Ub-E1_IAD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023318">
        <property type="entry name" value="Ub_act_enz_dom_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030661">
        <property type="entry name" value="Uba2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019572">
        <property type="entry name" value="UBA_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF5">
        <property type="entry name" value="SUMO-ACTIVATING ENZYME SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10585">
        <property type="entry name" value="UBA_E1_SCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039133">
        <property type="entry name" value="SUMO_E1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8 11 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="16">
        <location>
            <begin position="10"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-activating enzyme SCCH" evidence="17">
        <location>
            <begin position="340"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="588"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="588"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="12">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="28"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="60"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="123"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="450"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR039133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR039133-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR039133-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR039133-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF10585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW30863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="613" mass="68787" checksum="BCA701796909A256" modified="2011-11-16" version="1">MAKDSYLKKILGAENFSRIQSSKVLMVGAGGIGCELLKDLVLTGYGEIHIVDLDTITLSNLNRQFLFRQNDINKSKSLTVSKAVQHFNYLNAHLVSHHGNIMDTNKFPITWWEQFEYVFNALDNLEARRYVNKMCLFLKKPLMESGTTGFKGQIQPIYPYYSECFDCSTKETAKTYPVCTIRSSPTQPVHCITWAKEFLFHSLFDEVESDQNLTDPNQIRSETDNEAEIAFFQKESTELAELRHLITTADPPTFINELLVKIFKADIERLLLIDSIETRRGSRKPTPLDVVRYSSQLAGLLADVSNENILNLDTKMWSVLENIYVLYKSSEVLQERIVSGRESSISFDKDDEDTLNFVAAASNLRSSIFGIEIKSKFDIKEIAGNIIPAIATTNAIISGFACLAGTKYFTTPSHGTDYSAITHKSSTIFVGITPNKYITSGTLGPPNEKCPSDALVSRGVFKISPTDFTTLTLGWLIGQLENYGYSRDDISIQKSNSKLLYDIDYDDNVDLTLASMSGLKDGELILISDDSDQLESLELYINIAEKTSLPELVLRPKVTHDEPEQENEDGLLENGMDVENDGVILLDSEAENEENDDLLIIDDEPSPKRRKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATX6</accession>
    <name>G3ATX6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_142974</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377323.1">
        <property type="nucleotide sequence ID" value="XM_007377261.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3ATX6">
        <property type="interactions" value="852"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATX6"/>
    <dbReference type="GeneID" id="18870505"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142974"/>
    <dbReference type="eggNOG" id="KOG1240">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001696_0_1_1"/>
    <dbReference type="InParanoid" id="G3ATX6"/>
    <dbReference type="OMA" id="YNLLCSW"/>
    <dbReference type="OrthoDB" id="242910at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071561">
        <property type="term" value="C:nucleus-vacuole junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045162">
        <property type="entry name" value="Vps15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583:SF0">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="29"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW30352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1356" mass="156455" checksum="86F8554F8CC59180" modified="2011-11-16" version="1">MGQRLSLLAPSAPTVAISSYIDDALNNNYQYLELLSNSRFLKTIKAIDKTTGGLVIIKILIKPSTSSFNIQLQGLTEFLVKQSKAISPMININCYSKIIETDRAGYLIRQMIKTNLYDRLSIRPFLEPIEKSFIIFQLLKLVSQLHALNIHHGDLRLENCLITSWNWILLSDFSNMIKPTFVPMDNPNQYAFYFDGSSNRRICYLAPERFYKGQHLQKHSNFNDDGSFNNKETLTDAMDLFSLGCIIAELYSDGQPTFTLSQLFKYMEDPNKLDLAYINEENVIKLVMKLLKVNPNDRSTARELLDEFYDICFPEYFYTFLYDFMEGLNEGDSSDEKIEYIYDNFELISKALHFNYTNADESNGEFLSPIPTKLNLPTMPYNYSIKPRKPDLQPSLIILNAICSFCKTLTHVDSKLKAIELILAFSERISDESKLNRSLPYLFQFIDEYVEYYQQPQTEESHISCKVVSHTIHAITTLLSTITYITPINTLLFPEYLLPKLYNLLILKLDSNSRKMINCSIAICLPYLSTISQKFWIMSKAFATPPSKDQLYDKFKTLTLLLLTDSESTVKCNVLTNILPLCQFFGADKTNDIILPHLISYLNDHTNHELRLTFLAAILEIGPFIGIISFEQYLLPLLIQTLNDNQEMVILKVLQVFHTFTKNKFITNKVVYNEILNNSICLIIHPNQWIRHSIINLIISINENLTNVADSYCWLYPILQPYLTYDITIIDWNSLYTSLTRPLTIKIFEMSRDWSRSQRSLFWRNSSKTLSHEDRQWVIKLKSIGLEEKELWKIRKLREYIRHTHPAEQLESRFNNPGLINPRNIFFDISYKSEPMINSKTRQLSIDIEVDKPSSFLTPKVTASIQTMETNVFGELDGLPHSHKSSSVVNGHVPTRVFSINNSKIITTHIRHTYSGSNPYILNYLNGITLTPTLSDFPEFGSVHRQSKQQQQIEFKPRGSLVAHLKDEIDAIKCIEVVNTSEFFITGYESGIIKLWDSFKIEKIINSKNYQLSIDLKSEITCICFIPDRFVFVACTKDGQVRVFSVEVSRNKNRRIVRYVKFRLLRQVKIDGYILHAKFSNNFLVCVDSNSKILMFDIITMNKVKEVQNPLVHGIINTFFVEETWIITGTSSGIINLWDLRFNLLIKSYQLKYVMNETVYSVSSIKSFQVVKKEEGELTIAVSIGGDTSHTIVLLEIPNFEVKTILRVDQGDSSVERYFVEGTPNKTNIDDLLKDLDVNKPIKQNEVMAVRSILHYLICATYDKRIILWDLSNISSSTSINSQFQSRFVTRTVSNTVIIDEKLELKDMIESEHEQTTWSGASSKAHLDIINDLNYLYEPFEMIISGDRSGVLNLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATZ0</accession>
    <name>G3ATZ0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Alpha-1,6-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_63218</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377337.1">
        <property type="nucleotide sequence ID" value="XM_007377275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATZ0"/>
    <dbReference type="FunCoup" id="G3ATZ0">
        <property type="interactions" value="113"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATZ0"/>
    <dbReference type="GeneID" id="18874647"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63218"/>
    <dbReference type="eggNOG" id="ENOG502QW2I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022381_5_0_1"/>
    <dbReference type="InParanoid" id="G3ATZ0"/>
    <dbReference type="OMA" id="WIPENVS"/>
    <dbReference type="OrthoDB" id="411251at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.20:FF:000002">
        <property type="entry name" value="Initiation-specific alpha-1,6-mannosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039367">
        <property type="entry name" value="Och1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834:SF11">
        <property type="entry name" value="GLYCOSYLTRANSFERASE HOC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="399" mass="46342" checksum="EC1CF096AC231A6C" modified="2011-11-16" version="1">MAKYGSGRGIKRSWLIELVVLVVTGGFIVFTVSQSSKEISEVLPYIPNEKIFNFLSNENEKNQDLLLAKIEKLNRKLFQEQQARLDELEKQQRLLLKEIKSLKSPPAKASLRDKLVYLYPYDPQAKFPAYIWQTWKHGLNDERFDEKYRQGESQWAWKNPGFVHELFNDDTAHTMIRHLYKQIPEVIDAFESLPEVILRMDFFRYLILYAKGGVYADIDTFPLQPIPNWTPENVSPTDIGMIVAIDIDSSSPNWRQESARRLQFGQFVIQAKPGHPVLREVISEIVEQTQYKKTQLMENDKLKLTGSSNQKSLQISKWTGGALWTDVILKYLNDYIQSSIYQTITWQDFHDLEIPKLVSDILVLPIKSFASELEIPKDGKIQDPLAFVKHYSAKIWKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU11</accession>
    <name>G3AU11_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_143218</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377358.1">
        <property type="nucleotide sequence ID" value="XM_007377296.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AU11">
        <property type="interactions" value="477"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU11"/>
    <dbReference type="GeneID" id="18870526"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143218"/>
    <dbReference type="eggNOG" id="KOG1910">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000740_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU11"/>
    <dbReference type="OMA" id="PNYFAKP"/>
    <dbReference type="OrthoDB" id="1562405at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019441">
        <property type="entry name" value="FMP27/BLTP2/Hobbit_GFWDK_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019415">
        <property type="entry name" value="FMP27_SW_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019449">
        <property type="entry name" value="FMP27_WPPW_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045167">
        <property type="entry name" value="Hobbit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678">
        <property type="entry name" value="ANTIGEN MLAA-22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678:SF15">
        <property type="entry name" value="PROTEIN FMP27, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10344">
        <property type="entry name" value="Hobbit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01214">
        <property type="entry name" value="Fmp27_GFWDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01215">
        <property type="entry name" value="Fmp27_SW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01216">
        <property type="entry name" value="Fmp27_WPPW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27 SW motif-containing RBG unit" evidence="5">
        <location>
            <begin position="1108"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27/BLTP2/Hobbit GFWDK motif-containing RBG unit" evidence="4">
        <location>
            <begin position="1227"/>
            <end position="1380"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27 WPPW motif-containing RBG unit" evidence="6">
        <location>
            <begin position="1629"/>
            <end position="2054"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2379"/>
            <end position="2441"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1783"/>
            <end position="1834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2419"/>
            <end position="2431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="2570" mass="296415" checksum="E92E75FBA94468C1" modified="2011-11-16" version="1">MTIDYPHSDQIVYYFFVIFLGWYLVWKLIYTITGFHIGSISINNGFSFNNVSIRLKKANITLRSFRFRLWGNTRMIIIDDLAVVLHSSKGNNLNKGNCSDTAKPELDIPNSLNIFPSNRLLRYITRLVICHLPTIDIELRHSTFNSSSNDKYTIDYLRFTLLTRYSKRHSDKVKINNEVTINDVVLHTTTSIGEVINPISISGVKFQIKFSVNYNDGKLGELNTRINVNGSKLSVFNFIKYYEILSFIDDDESVSARTPEVVDHEKRRAIRRVEVFYRTVHAIVTDFNVNLQNCRVTDIPFATLENNINMKEYFQADTPKTSLEVHTKSISFNFARLFTESAGFEVLFNPKVDRPYHLTCSVHMLRAFFSQQTKLADGSLGRDDDEILNIPNYALTYKTNILDQIVKAKGFKNCVVEVYFSASTPIIDLSTRQLSSVIYNLVLLRKFMRLKQIKQLIADVGANDYISDLEDAYDITNVDLEGDTASTFSTESSKHEPLRAIIWKYLNDYYPHLDIKLVIEQPRLVLRHCEGPKQNIQILTFSYALLNFSMSTTPSRDYEASCQVLSPNVEYQEKSGLLAEEFNNEIMRKQVGTLSYFNLKFDVVKNLKVKMFMHLNELSVDLTNIDIFKGIHYLSVDLKKMAETDIEIGVINIALNRQLNELRQRILSQKVGKANQLRHLSIEDELFKPLPSWLTEIDLSLSRMNISLGSRSVLIGKDELLETEGDGLMYDFSDNHELRKINLKMDHGNINILNNSSQKADFSTPSSSVSGSTESLNTLTSAGRDIVYWSVGTKLENISVSTHSDIDSRRAKYSNILVIDCAKQVVKAVCDSNHHNTLVVEAEVDSVSINYDRYKLFTLIGAVYLVNEYVIRPLKLIHLKLNMDLTRFNNPLTPEISIPERSLSSLLSYKLKLGSVDVVLGLTDNLKIKLQAVQTMVNVIDKDVTVSIDLVRLLADSPFIKGKWCRLCCLDTLGLDFGIPQSLTDLKIELNTYAITFLQPHNFIMYEVFDNVAILWKLSKNLVRILNQRGSKEGSNVIHPKLEKALRLPWLNVKSKHVKFMMEDDPFESQLNMIYQLGKVEQAKRQELYSLFEARAEEEQLDSEEYFIKLDYLNKTVSSSWIRKVRVFRTQLKQEVLNNRKYLFGMDADFESSLNDDVIPYSYEPPLLSFYLDNFDLRLSMPRFDIKKLPKFIYEMGQGVPKDTKYSLLIPMFVDLRLGELRMHLRDYPLPLWHAPRNKDKSNPSLTLDGHIVFTKTYSTAPEQLKITNYQMTPFSNKGNKSPFDTISIEKVIAKVKTYMDLNWHFNSDYPTRFIWGTSYNFGIQQFMLNFDQFSSPILDPSKKLGFWDKIKLIFHGRFKIKTRKSLEIGFKGSRDPYQLFSTASGFVLSFRKNVVWTINEKDDPTSFFDIHAENIAWYIPNYLGAPLLSWTRKSKDSIYLPDSPSLISSFFGYYLEPSTGKRVDFDFAKKVIGKTVINLSGGVSYRVGFSFQRRDESGMRTDTFIPHYKLELYNPDYCEPEHDSYKGFRSEFIHMSYSVNASSDHSYNTVHLSPGVFHQFFSWWKLFSGNMMLPIRKGIMFEEASRSVKFSQHLVTNKFSFYLKSLFISHIHRDELMDMKEDKVECVGLRAKVDLFSMDLHQRKEPRVEYNEELSKNSRIMKMNFNIGEIHLFGIDLRVVQASFIQNMYSQQQQNMYDDSASKYDIFDNDKKWFDIRDYQEGFLPSLANCERTVKIAPLVFCHRFTYDRDTHSDNNFKTDPFGDEEIHDCRLGKLNPFEPQIEVLNSRINALEKQIKKNTKKGSSTKQLKQRIEFLEKEIQKLKNEWKGSGRRQSITSKLDDIEYYHNMFTIFSMLLKWNFTSRNLVLRYIHFIQFRTSMYKFLSHESIATLERIIDRSQEFLQGDDVSVSSDVISRIAKSRRSAEFSKHKASTSQFRLDNFDKILKKKRITEKFTEDYLIQFISPQIQLQSGDCPDSVVLISAPSIDGKILSIIDNSLTSELLETRYGVVVRNASVFVLNKKDIIGSDDLVISEMHYGAKSAWPPWLGTEITLNGKWAGQNELLIEDLSVMLLYHEAEILGGKLAQINDATSHFSELQSADFADAHEDVDAPKKLRIDAPSVVITSTSSQYFTLYVIVLSLLFYSEPMSKVIHHKLEKLKFSIDFQDLASLSDRLIQMQTYYKLLNMLSHNYNFRQHELSNEDLNNYLSLNLEKGEISSDIYLLFKTLLTGELLSDDSSKAMQRFWLVRADEIILHILEDDRTPIMDLALARGTYKRKELEGGSNINRVEIGMMQGFNLIPHARYPDFIQPFEFDEKTQGNMIDAVWTMNRSVGGIKIVENIEINSMPLNIKLDEKTGRKLMDFIFQSKESPILEITHDSRRTNIENESDCEVESGNGNGNGKGVKFELGSEDSSRGKGSSGNSKNKTNMPEEDEAEDTQIQTMIERSKTYFSVVSLKVNSISLQVSLKLNKGYKRLFNVQDLHLDMPEIHIQHQIMSLLEITKVFEKQITKTLLNHVGKLISNKFSSMKTPTKSLSHLKQLKQYREFTNVRDLADTDATDDSRVHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU34</accession>
    <name>G3AU34_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_63260</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377381.1">
        <property type="nucleotide sequence ID" value="XM_007377319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU34"/>
    <dbReference type="FunCoup" id="G3AU34">
        <property type="interactions" value="99"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU34"/>
    <dbReference type="GeneID" id="18874663"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63260"/>
    <dbReference type="eggNOG" id="KOG0750">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053371_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU34"/>
    <dbReference type="OMA" id="GQPWFSD"/>
    <dbReference type="OrthoDB" id="409947at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001409">
        <property type="term" value="F:guanine nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053042">
        <property type="entry name" value="Mito_GTP/GDP_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46974">
        <property type="entry name" value="MITOCHONDRIAL GTP/GDP CARRIER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46974:SF1">
        <property type="entry name" value="MITOCHONDRIAL GTP_GDP CARRIER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="7">
        <location>
            <begin position="122"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW30410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="257" mass="27725" checksum="42638B931A4B9ED9" modified="2011-11-16" version="1" fragment="single">MSPSAHSSSDKKQSGSARVLGSAAAGICEIAVFHPVDTVSKRLMSNHGSIKSAAHLNQVIFRDAAEQALGKRLFTLFPGLGYAACYKILQRVYKYGGQPFANEFLSKNFKDSFDDAFGPKNGKALMSATAGSLIGIGEIVLLPLDVLKIKRQTNPEAFKGRGFTKIIADEGFGLYRGWGWTAARNAPGSFALFGGNSFAKEYIFGLKDYSQATWTQNFITSIFGASASLIVSAPLDVIKTRIQNKNFNDAQSGFTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUE1</accession>
    <name>G3AUE1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA(Ile)-lysidine synthetase</fullName>
            <ecNumber evidence="1">6.3.4.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_157650</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cytidine(34) in tRNA(Ile2) + L-lysine + ATP = lysidine(34) in tRNA(Ile2) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43744"/>
            <dbReference type="Rhea" id="RHEA-COMP:10625"/>
            <dbReference type="Rhea" id="RHEA-COMP:10670"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:83665"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.19"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.3.4.19" evidence="1"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377488.1">
        <property type="nucleotide sequence ID" value="XM_007377426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUE1"/>
    <dbReference type="STRING" id="619300.G3AUE1"/>
    <dbReference type="GeneID" id="18871091"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157650"/>
    <dbReference type="eggNOG" id="ENOG502QQNE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015599_1_0_1"/>
    <dbReference type="InParanoid" id="G3AUE1"/>
    <dbReference type="OMA" id="MCLTHLL"/>
    <dbReference type="OrthoDB" id="434144at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032267">
        <property type="term" value="F:tRNA(Ile)-lysidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01992">
        <property type="entry name" value="TilS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01161">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012094">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012795">
        <property type="entry name" value="tRNA_Ile_lys_synt_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02432">
        <property type="entry name" value="lysidine_TilS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43033">
        <property type="entry name" value="TRNA(ILE)-LYSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43033:SF1">
        <property type="entry name" value="TRNA(ILE)-LYSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal" evidence="7">
        <location>
            <begin position="27"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="486" mass="57146" checksum="DBB0802DCC103066" modified="2011-11-16" version="1">MNRISLDAFSNVLRKYFNTADVLPRRVAIALSGGADSMLLTYLLTQYKHLHSPHTEIYAITIDHKYRPESTDETKHIRDIVKQWDVHHVVKTLSYDQEVSSIGNFEEVARHKRYLKFENVCKSKQISALFMGHHLDDQLETFIQRLQGNSSLYGLAGTRKITRLPRNDLSPIDNHHSVFVYRPFWQFEKRDIVDTCQFNGVRYFNDPTNQDIDLTRRNYLRHLIGDVIPAKITKHSQYSCISKQALRDSHEQVYNLAESFEEKANNIKNMIEKNGWIRTNDAMSSLNVKIPRRLFAKPNEIVLGKYFYQILYPYSTLKHYHWAYAKLERQLVPKIQRFLDTDDTTFKTTMMNLTFDINIIEEDILDIAIKRQPIVTGDDVSLSVDVTDKWSEYVLFDRRFWVRFCSAENMQVKVIPYMHKKCKPRLAQDLLKSKQVNSKLNTLPAVMYHHEIIAFPSIGLVSEKYDSFRMECSLKENKYGRLEQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUK9</accession>
    <name>G3AUK9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Oligopeptide transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_63401</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377536.1">
        <property type="nucleotide sequence ID" value="XM_007377474.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUK9"/>
    <dbReference type="FunCoup" id="G3AUK9">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUK9"/>
    <dbReference type="GeneID" id="18874732"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63401"/>
    <dbReference type="eggNOG" id="ENOG502QQ2H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010539_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUK9"/>
    <dbReference type="OMA" id="CVVSPWL"/>
    <dbReference type="OrthoDB" id="627262at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645:SF0">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="317"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="417" mass="44484" checksum="AD6063CB2E2174B4" modified="2011-11-16" version="1">MGLPTVSYMLFGAILGWGILAPLSKYMGWAPGEINDWINGGQGWILWISLSVMISDSLVSFIIVSGRSLIGAYRLINNNNGVIGTTTATTTTTTTTTAATYYPLLPATSGSIGSNDDPTSSAYEYKENVNLITPVADKYLISNTTAVIGIIASTLLCVLCLHLVFGTIVPLYASFVAIILAMLFSVLGVRALGETDLNPVSGIGKLSQLIFAVVIPPSHPARVLINLVAGGIAEAGAQQAGDLMQDLKTGHLIGASPRAQFTAQVIGTIYSVFLSSIVYKLYNSVYTIPNETFRIPTAIIWIDCSRLVTGKGLPPHAYEFSIVFGVIFAIISLLKNTVPKESKYHKYLVYLPSGVAVGIGIYNTPNFTLARFIGGVVNHWWTNKGMDRISMIIFSSGLVLGEGLLSVVTMGLASMGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUN9</accession>
    <name>G3AUN9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein ORM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_143516</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377566.1">
        <property type="nucleotide sequence ID" value="XM_007377504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUN9"/>
    <dbReference type="FunCoup" id="G3AUN9">
        <property type="interactions" value="541"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUN9"/>
    <dbReference type="GeneID" id="18870551"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143516"/>
    <dbReference type="eggNOG" id="KOG3319">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072117_2_1_1"/>
    <dbReference type="InParanoid" id="G3AUN9"/>
    <dbReference type="OMA" id="STHYTHF"/>
    <dbReference type="OrthoDB" id="1932233at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007203">
        <property type="entry name" value="ORMDL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12665">
        <property type="entry name" value="ORMDL PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04061">
        <property type="entry name" value="ORMDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018147">
        <property type="entry name" value="ORMDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="27520" checksum="E1CB17AC3B8B7A71" modified="2011-11-16" version="1">MPILNNRSPAEVNHLGPNDNLSSPSTPSSASSLSPHQVNNFSGHKRQLSFDVSPSRHRSNSNTLSPSTSHQSAHITRQRRSSSIIQHLEPDTLDTKVDQALNPNVNANWVHQKGAWIIHIVLIIFLKIFFNFISVLDNDWKWTLTNLTYNIGSYIMFHQVKGTPFEFNSGAYDNLTMWEQIDNGDQYTPSKKFLMLVPICLFLISTHYSRYNLNLFILNGISCLCVVVPKMAIAHRLRVTLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUP0</accession>
    <name>G3AUP0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="9">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_63436</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 9">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 9">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 9">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="9"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377567.1">
        <property type="nucleotide sequence ID" value="XM_007377505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUP0"/>
    <dbReference type="FunCoup" id="G3AUP0">
        <property type="interactions" value="888"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUP0"/>
    <dbReference type="GeneID" id="18874748"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63436"/>
    <dbReference type="eggNOG" id="KOG2467">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022477_1_0_1"/>
    <dbReference type="InParanoid" id="G3AUP0"/>
    <dbReference type="OMA" id="WFRGVQY"/>
    <dbReference type="OrthoDB" id="10265628at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000097">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF28">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="10">
        <location>
            <begin position="17"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="307" mass="34009" checksum="4C816C10D5EC27DC" modified="2011-11-16" version="1" fragment="single">MTYALSATHRQLTEGSLKETDPEVASIIKDEIDRQQHSIVLIASENFTTRAVFDALGTPMCNKYSEGYPGARYYGGNEHIDRIETLCQERALKAFGLTADKWGVNVQTLSGSPANLQVYQAIMKPHERLMGLDLPHGGHLSHGYQTDSRKISAVSTYFETMPYRVDLETGLIDYDMLEKTAVLFRPKVLVAGTSAYCRLIDYKRMREIADKVGAYLVVDMAHISGLIAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRRGVRSVNPKTGQEILYDLENPINFSVFPGHQGGPHNHTITALATAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUY6</accession>
    <name>G3AUY6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thiol-specific monooxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_63462</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FMO family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377610.1">
        <property type="nucleotide sequence ID" value="XM_007377548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUY6"/>
    <dbReference type="STRING" id="619300.G3AUY6"/>
    <dbReference type="GeneID" id="18874765"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63462"/>
    <dbReference type="eggNOG" id="KOG1399">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006909_5_0_1"/>
    <dbReference type="InParanoid" id="G3AUY6"/>
    <dbReference type="OMA" id="GRYNAYF"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW29844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="340" mass="38795" checksum="41C3A4720E29F17F" modified="2011-11-16" version="1" fragment="single">SITMAIKSVAIIGAGPSGLISLDSLIKEQKFDLIRVFERRKEAGGCWIYDPQPPEPIKSFDKLSKGEIEIDEIPQVLPTYVPKSHTQRFMDTATYSYLESNVEATSMEFSQETFPNQASKLSKSKYGENTPFRHNSILKKFVTDLYKTRGYDDHIEFNCSVELVEKVNDKWRIVLRKFGKNQDYIWEEFFDAVIVASGHYDVPYVPSIPGLQEFSDLPSTTVIHSKAFRSREQFRNKKTIVVGASVSAMDSVQDIVDVVKKPAISSQKKTSGPHVYFGTEAFDHPHIQKRTQIVKIETETRTVYFDDDTTESDIDAIIFATGFIYKYPFLPNFNVSNHRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV24</accession>
    <name>G3AV24_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutamate--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Glutamyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_63719</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Glu) + L-glutamate + ATP = L-glutamyl-tRNA(Glu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="3"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377865.1">
        <property type="nucleotide sequence ID" value="XM_007377803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV24"/>
    <dbReference type="FunCoup" id="G3AV24">
        <property type="interactions" value="842"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV24"/>
    <dbReference type="GeneID" id="18874881"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63719"/>
    <dbReference type="eggNOG" id="KOG1147">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001882_1_2_1"/>
    <dbReference type="InParanoid" id="G3AV24"/>
    <dbReference type="OMA" id="ANRYFFV"/>
    <dbReference type="OrthoDB" id="10250478at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017102">
        <property type="term" value="C:methionyl glutamyl tRNA synthetase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000070">
        <property type="entry name" value="Bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1160.10:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.800.10:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000004">
        <property type="entry name" value="Glutamyl-tRNA synthetase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1160.10">
        <property type="entry name" value="Glutamyl-trna Synthetase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.800.10">
        <property type="entry name" value="Glutamyl-tRNA Synthetase, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02076">
        <property type="entry name" value="Glu_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050132">
        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004526">
        <property type="entry name" value="Glu-tRNA-synth_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020061">
        <property type="entry name" value="Glu_tRNA_lig_a-bdl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00463">
        <property type="entry name" value="gltX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF5">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9 13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="14">
        <location>
            <begin position="93"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib anti-codon binding" evidence="15">
        <location>
            <begin position="401"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA synthetases class I (E and Q) anti-codon binding" evidence="16">
        <location>
            <begin position="505"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW30099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="604" mass="69113" checksum="0E4D6DD62F1F74B7" modified="2011-11-16" version="1">MVGFNYSIADITVWSVLRANALMGSVLKHQVYANVSRWYALLASDSRFESQVEFMTKSVNELRKAAKSAQNGGKKETHKANFDIELPDAQMGKVVTRFPPEPSGYLHIGHAKAAILNEYFAHNYKGKLIIRFDDTNPTKEKEEFQDSIVEDLALLGVKGDVVSYSSDYFDKMYELAVKMIKDGNAYCDDTQLEKMREQRMVGEPSERRNRTVEENLQIFTEEMKNGTEVGLKNCVRAKIDYTALNKTLRDPVIYRCNLTPHHRTGDKWKMYPTYDFCAPVVDSIEGVTHALRTNEYRDRNAQYEWIINALGLRPVHIWDFARVNFVRTLLSKRKLQWFVDKQYVSNWDDPRFPTVRGVRRRGMTVEGLRNFIIAQGPSRNIINLDWSVIWALNKKVIDPVAPRFTAVEAENAVVVNLDGPAEPYTETKPKHKKNPDVGTKPVVYANKVLIDQTDAKELADNEEVTFMDWGNVIVTKVHKNTEGVVTSVDAKLHLEGDFRKTSKKLTWLADTDDKVEVDLVDFDHLITKDKLEEGDDFENFITPETEFHTKAVADLNVGKLKVGDVIQFERKGYYRVDRAYEQGKPVVLFTIPDGKTVSKYGAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV35</accession>
    <name>G3AV35_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GPN-loop GTPase</fullName>
            <ecNumber evidence="7">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_52939</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Binds to RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="7"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377875.1">
        <property type="nucleotide sequence ID" value="XM_007377813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV35"/>
    <dbReference type="FunCoup" id="G3AV35">
        <property type="interactions" value="902"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV35"/>
    <dbReference type="GeneID" id="18871739"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52939"/>
    <dbReference type="eggNOG" id="KOG1532">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037460_1_2_1"/>
    <dbReference type="InParanoid" id="G3AV35"/>
    <dbReference type="OMA" id="MIIVFNK"/>
    <dbReference type="OrthoDB" id="243313at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17870">
        <property type="entry name" value="GPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000579">
        <property type="entry name" value="GPN-loop GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030230">
        <property type="entry name" value="Gpn1/Npa3/XAB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF8">
        <property type="entry name" value="GPN-LOOP GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="293"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="294"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="321"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW30109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="372" mass="41753" checksum="047128618C6783C9" modified="2011-11-16" version="1">MAPSTVICIGMAGSGKTTFMQRLNSHLHSKKTPPYVINLDPAVLKIPFGANIDIRDSVKYKQVIEQYNLGPNGAIVTSLNLFSTKIDQVIKLIEKKGDSVNNVIIDTPGQIECFIWSASGAIITEAFASTFPTVVAYIVDTPRNTSPATFMSNMLYACSILYKTKLPMIVVFNKTDVTDCQFAKDWMSDFEVFQQALKDHENEDEGSGYMNSLINSMSLMLEEFYSQLDVVGVSSYTGDGFDEFMQAVDNKVDEYNEFYKAERERILAKKAEDEQKRKASSLNKLMKDMKIDGDTDVLSDYEDEPDENQGEVLRDEDDVEREYTFPEDRNSEVNEQTDADLQARYQAAFESTAKTASSKTAESIAAYINQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV51</accession>
    <name>G3AV51_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_57576</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377891.1">
        <property type="nucleotide sequence ID" value="XM_007377829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV51"/>
    <dbReference type="FunCoup" id="G3AV51">
        <property type="interactions" value="1200"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV51"/>
    <dbReference type="GeneID" id="18872130"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57576"/>
    <dbReference type="eggNOG" id="KOG1466">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016218_0_2_1"/>
    <dbReference type="InParanoid" id="G3AV51"/>
    <dbReference type="OMA" id="GDWESCK"/>
    <dbReference type="OrthoDB" id="10249309at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002183">
        <property type="term" value="P:cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903833">
        <property type="term" value="P:positive regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900036">
        <property type="term" value="P:positive regulation of cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045948">
        <property type="term" value="P:positive regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1070:FF:000002">
        <property type="entry name" value="Translation initiation factor eIF-2B subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1070">
        <property type="entry name" value="Translation initiation factor eIF-2B, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051501">
        <property type="entry name" value="eIF2B_alpha/beta/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042528">
        <property type="entry name" value="elF-2B_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="307" mass="34445" checksum="C618020726D04618" modified="2011-11-16" version="1">MTEFDITQTYLKFLEEDKDMTMPIAAIESLVSMLSIKQPSTSSELISLIQSNIDKLKSSIPNNISLSAGCDLFMRFVLRNTNIYSDWESFSQNLVENGQLFVLRAKESREKSAEFGVSFIKDDDTVLVHSYSRVVYNLLVKAKKEKLIRFKVLVTESRPTGSGYYMAEKLRQADIPVDVIVDNAVGYVIHKVDKILVGAEGVAESGGIINHIGSYQIGCLAKVNNKPFYVVTESHKFVRLFPLAPDDLPNKMNHLIAKANEQEETTNDLKSHFVDFTPSSYITALITDLGVLTPSAVSEELIKIWYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV53</accession>
    <name>G3AV53_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="10">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_52956</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="10"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377893.1">
        <property type="nucleotide sequence ID" value="XM_007377831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV53"/>
    <dbReference type="FunCoup" id="G3AV53">
        <property type="interactions" value="791"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV53"/>
    <dbReference type="GeneID" id="18871741"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52956"/>
    <dbReference type="eggNOG" id="KOG0348">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_26_2_1"/>
    <dbReference type="InParanoid" id="G3AV53"/>
    <dbReference type="OMA" id="AVHIKAD"/>
    <dbReference type="OrthoDB" id="422663at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000464">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17949">
        <property type="entry name" value="DEADc_DDX31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="174"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="390"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="692"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW30127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="734" mass="81521" checksum="21EB3F8D1401B9E6" modified="2011-11-16" version="1">MSEDDGLLLNFASADTPSVSSKKTAKVSGGNWKERRKLQLRLTGRGANKKNDASGVNKITVDSSRMERVKQANKEAGKDRGSDSGSSRKRVKFAESKGESGGKDNSYVSSLFTSNAETTTLKPTTSSKTYDASNAPLSDSTTFPGLGINEKLSTHLTDHLRFKHPTKIQRMVIPTLLAQDNDVFVKAQTGSGKTLAFLLPLFHRLMSEDITRESGLFAVILTPTRELATQIYGVLETLTRCHHRIVPGIVIGGEKKKSEKARLRKGVNILVATPGRLADHLENTKTLDVSQLRYLILDEGDKLVELGFEETITSITNKISQNSRIHETLTKWKGLPSKRINVLCSATMQNNVEKLGSIILQNPTMIQVEKQHIGTVEFEESEVGNTAPDQLIQNIVVVPPKLRLVTLNAMLSKYIRSSEASRTIVFFSCSDSVNFHFEVFTRNGKNPNKKQAAEDIEEEEEEEANILTAPGLSSSTTVYKLHGSLSQKIRTSTLNHFIKDTTPHQILFCTDVASRGLDLPNIANVIEYDPPFTIDDHLHRIGRSARLGNKGNATLFLMPGIEEAYVDGKLRVVHPNEGSLRILNYEQILEDGFAEVQEEGTAAAAAPAKKNDPKRKTGKWDIHATTWHLDIERWLLEDTGAHDRAGHAFTSHIRAYATHLSSERDYFNVKLLHLGHLAKSFGLRETPKKLGKSINHESVSTGTTKRRKKDEDPRKKMLRMARLAVNSASSEFNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVF9</accession>
    <name>G3AVF9_SPAPN</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_53004</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007377883.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="18871746"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53004"/>
    <dbReference type="HOGENOM" id="CLU_1750824_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVF9"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="149" mass="16326" checksum="50E265F743E347D9" modified="2011-11-16" version="1">MTKKARGPLLSSSIKDSRSNNQETSKQPMTSSMSTTPSAASLLANTANSRNSLSNYPTFNSNYKRHTTTNLSISSNSKQLKLNTEAVNCKSSLKFKSVGTQTTESALFQDKRVKYVDDDAYNSQLSSLFATFNFGSKQDLVNDLTTSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVJ4</accession>
    <name>G3AVJ4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_144303</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007377647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVJ4"/>
    <dbReference type="GeneID" id="18870614"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144303"/>
    <dbReference type="eggNOG" id="ENOG502RQ32">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120068_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVJ4"/>
    <dbReference type="OMA" id="HENYFGM"/>
    <dbReference type="OrthoDB" id="3997547at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW29943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="188" mass="21924" checksum="0D1B1288DDA082DD" modified="2011-11-16" version="1">MSYTTKRGREDFEPDTKKIKNYINQQSHQEITESNEWPQFAHPEGGIVKITPWGSLRQFVDPISGESITKFEDMSFEDYTFKASNVEDIYTVKHQEPPSMPSTPQSLYQTHSYQPNTRFASHNMPGGNSPLRLSPECEAEGYLRDGYRGQQAQQQFEQEHENYFGMHDQQEEFDCSEINYSIEDDEMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVK0</accession>
    <name>G3AVK0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Programmed cell death protein 2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_63575</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377715.1">
        <property type="nucleotide sequence ID" value="XM_007377653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVK0"/>
    <dbReference type="FunCoup" id="G3AVK0">
        <property type="interactions" value="345"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVK0"/>
    <dbReference type="GeneID" id="18874813"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63575"/>
    <dbReference type="eggNOG" id="KOG2061">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031771_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVK0"/>
    <dbReference type="OMA" id="MPGPWAD"/>
    <dbReference type="OrthoDB" id="443682at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140597">
        <property type="term" value="F:protein carrier chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007320">
        <property type="entry name" value="PDCD2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47524">
        <property type="entry name" value="20S RRNA ACCUMULATION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47524:SF1">
        <property type="entry name" value="20S RRNA ACCUMULATION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04194">
        <property type="entry name" value="PDCD2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Programmed cell death protein 2 C-terminal" evidence="2">
        <location>
            <begin position="300"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="275"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW29949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="429" mass="48239" checksum="3E41735D4CAD60D8" modified="2011-11-16" version="1">MSSHDEYSSDEEDLFDESQKSDVFLGFVDAPITEEEVPTIEDTFIGGQPIWLHPQSQPNEKSLTCDNCNKKMALLLQAFAPMDGKLYDRVIYIFGCKDTRQCSKKKGSIKAIRGIVKDQETIDKIKKETEALQAKQLDEKLKADNQKKLNIELTKDLFGKKEESSNPFGGGSNPFDNKNDNPFAAGSTSPFGANPFGAKAEEEPKKKPESYAKIASKNEPKKAPKKKTVSDLPSYKGSFVYVDTEKFKKATNDPELEKYKHLIDMDVNKDDDSESVGASRRGSSSVLDPQTSKISNMLDDKYFEAFSNTVKHNPGQVLRYNLGGKPLLYNGKDDVAKKFLSQQPANIPQPAYNPSSERQYELQLMPKAIMDLEEINTNTVQISDILNGMSWGTIIVATDKEDYMPEQEFDENHVAYIEEWCGVQWEESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVL1</accession>
    <name>G3AVL1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor TFIIIC triple barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_52811</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377726.1">
        <property type="nucleotide sequence ID" value="XM_007377664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVL1"/>
    <dbReference type="GeneID" id="18871722"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52811"/>
    <dbReference type="HOGENOM" id="CLU_1679043_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVL1"/>
    <dbReference type="OrthoDB" id="4095947at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW29960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="17777" checksum="20809B3BE029C632" modified="2011-11-16" version="1">MIKPHANFQYTDLSQPNPFVKLSNNSTGNALDSVGGEELLNSMNLIDGKIFESSWKSLVGTELIFDDYGELIGRVKEHLVCNENVKLVPKREEIKKEPRKKKPDEDAKMDVDVDADADVTTETSQAEEKPADNSIPTEFQTQFFKRAMKEAQKKEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVN1</accession>
    <name>G3AVN1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SGF29 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_63808</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377962.1">
        <property type="nucleotide sequence ID" value="XM_007377900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVN1"/>
    <dbReference type="FunCoup" id="G3AVN1">
        <property type="interactions" value="390"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVN1"/>
    <dbReference type="GeneID" id="18874925"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63808"/>
    <dbReference type="eggNOG" id="KOG3038">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023535_2_0_1"/>
    <dbReference type="InParanoid" id="G3AVN1"/>
    <dbReference type="OMA" id="VGRSYWT"/>
    <dbReference type="OrthoDB" id="10265994at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140671">
        <property type="term" value="C:ADA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140002">
        <property type="term" value="F:histone H3K4me3 reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072742">
        <property type="term" value="P:SAGA complex localization to transcription regulatory region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd20393">
        <property type="entry name" value="Tudor_SGF29_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20394">
        <property type="entry name" value="Tudor_SGF29_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000055">
        <property type="entry name" value="SAGA complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037802">
        <property type="entry name" value="SGF29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010750">
        <property type="entry name" value="SGF29_tudor-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047288">
        <property type="entry name" value="Tudor_SGF29_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047287">
        <property type="entry name" value="Tudor_SGF29_rpt2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21539">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21539:SF0">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07039">
        <property type="entry name" value="SGF29_Tudor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51518">
        <property type="entry name" value="SGF29_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SGF29 C-terminal" evidence="6">
        <location>
            <begin position="118"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="79"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="249" mass="28358" checksum="99F20240895BE78E" modified="2011-11-16" version="1">MSVDLQGNWDIVVTSLQDICNANETITFNNIYKGVSNSTLQQDIIDHKSNIGTGKRLIKTSQQSLKLIIDKLSEEQAKPATKSIAKKNLHRSSKTSTSKKNVSTRKSGRSFWTSKFNPNEPIVVSSEVAYKLKNRHFEEWIQCEVMKVLGDGNKFEIRDPEPDENNNPGQTFKATYKEILLIPDEDSELLVYPYGTKVLARYPETTTFYPAIVVGTRKDGRVRLKFDGEDEVNKETEVERRLVLPFPDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVV0</accession>
    <name>G3AVV0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein bL27m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">54S ribosomal protein L2, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_63621</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL27 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377761.1">
        <property type="nucleotide sequence ID" value="XM_007377699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVV0"/>
    <dbReference type="FunCoup" id="G3AVV0">
        <property type="interactions" value="281"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVV0"/>
    <dbReference type="GeneID" id="18874835"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63621"/>
    <dbReference type="eggNOG" id="KOG4600">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063752_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVV0"/>
    <dbReference type="OMA" id="YLDPFHP"/>
    <dbReference type="OrthoDB" id="1867012at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000042">
        <property type="entry name" value="50S ribosomal protein L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001684">
        <property type="entry name" value="Ribosomal_bL27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018261">
        <property type="entry name" value="Ribosomal_bL27_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041244">
        <property type="entry name" value="Ribosomal_bL27m_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00062">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15893:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL27M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15893">
        <property type="entry name" value="RIBOSOMAL PROTEIN L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01016">
        <property type="entry name" value="Ribosomal_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18471">
        <property type="entry name" value="Ribosomal_L27_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00063">
        <property type="entry name" value="RIBOSOMALL27"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110324">
        <property type="entry name" value="Ribosomal L27 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00831">
        <property type="entry name" value="RIBOSOMAL_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Large ribosomal subunit protein bL27m C-terminal" evidence="10">
        <location>
            <begin position="138"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="41"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW29995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="295" mass="33731" checksum="AAD32827618A921D" modified="2011-11-16" version="1" fragment="single">MSSLAKSILGSSRVIIGLTANPVNFIQIRTATKRVASSRTNNKDSPGKRLGPKESDGSFVKPGHIIMRQRGTKIHPGENVRIGKDHTIYAVEPGFVRYYRDPFHPLRKYVGVALRRDLTLPKDHFEARVRRFGYIEIKDPEAAEREENVKSRKELLYQPELERKLKEKEEVSKANLSAFSKGLVNDFKLELSDEELSLASSRLLAVFELSQTGQTWEAAQTQETFSQIYLLKLRAKRGEISQEEFETLKKQYIEITSKIDNEVSVSWDGKLCKYLNPEELSKKKQELKASMESLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADW2</accession>
    <name>G3ADW2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_57735</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372098.1">
        <property type="nucleotide sequence ID" value="XM_007372036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ADW2"/>
    <dbReference type="GeneID" id="18872175"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57735"/>
    <dbReference type="HOGENOM" id="CLU_2782992_0_0_1"/>
    <dbReference type="InParanoid" id="G3ADW2"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="69" mass="8151" checksum="3B829B57BF1C40B2" modified="2011-11-16" version="1" fragment="single">MSIWCRINSQKKKITTSIKVYNRDMSNCVYDSSRPPTDLHQSAKSVEICWVSDMPLWFSNFPKIWIPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADY0</accession>
    <name>G3ADY0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Maintenance of telomere capping protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_69123</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372115.1">
        <property type="nucleotide sequence ID" value="XM_007372053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ADY0"/>
    <dbReference type="FunCoup" id="G3ADY0">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ADY0"/>
    <dbReference type="GeneID" id="18875256"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_69123"/>
    <dbReference type="eggNOG" id="ENOG502QXZI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024520_0_0_1"/>
    <dbReference type="InParanoid" id="G3ADY0"/>
    <dbReference type="OMA" id="RTSHWDE"/>
    <dbReference type="OrthoDB" id="4064064at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038769">
        <property type="entry name" value="MTC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38426">
        <property type="entry name" value="MAINTENANCE OF TELOMERE CAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38426:SF1">
        <property type="entry name" value="MAINTENANCE OF TELOMERE CAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="588"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="621" mass="71397" checksum="895A7DCAC9FC3219" modified="2011-11-16" version="1">MSQPGATRLASGNSVASQLRLSRVTTPDSTLHRQPSKNSPKSKHDPEQEQQYNDVKPRKKHGAGFDLDITVEEASELTHLFLDTRNTLRADCNIIDPVTSDLPSSPQEMSPSKFSRSTIVRAEKVKTMIGMKYLYIQRIYEWNDAHGMNTEHPGIEGVYNPLQILRNRKIRAKYKEFPKPLSSKTIPLACIVFSKHNQGHHRKRTWRMVWAIELNEFVSDSRWRARHWHELEKPNGELWFPPEDHVPQPKSKRKKLRRLHDKLFDSVSDYEDKKSTHRRNESVVTAGSSDSELNMLKIHRSKSPKKKLRNKMKKFYERDSSSSAADIEEVSDHHEILNTQMFRRVVHSESSSDVAAPDDPKLASISPPIIKIEALPDESIQDVEFVPSKRSSSSPALIPSDLEEEEEEDVSTNSVPSEESPELMCTVDQREHAFSKIVSSVDYLYEVCVLRTNHLLVTYPNNLQVIQNKVDSITGKQTFDILRQMSSINDEHLPVYEELYHGFLDEIKSIIHMVNDNYSVKIDSLLSSSDRAISEINASLSLELRKVNERLDHLDESFSKKTIRPSDLKMNMKEGTNYKIMYFCLENLIVILLRLIWVLVNIFKGGVMVVGVVYKIIRLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE41</accession>
    <name>G3AE41_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">GDH3</name>
        <name evidence="11" type="ORF">SPAPADRAFT_146969</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 9">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372987.1">
        <property type="nucleotide sequence ID" value="XM_007372925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE41"/>
    <dbReference type="FunCoup" id="G3AE41">
        <property type="interactions" value="796"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AE41"/>
    <dbReference type="GeneID" id="18870737"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_146969"/>
    <dbReference type="eggNOG" id="KOG2250">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025763_2_1_1"/>
    <dbReference type="InParanoid" id="G3AE41"/>
    <dbReference type="OMA" id="MIMGWMM"/>
    <dbReference type="OrthoDB" id="6718861at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004354">
        <property type="term" value="F:glutamate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05313">
        <property type="entry name" value="NAD_bind_2_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.285.10:FF:000001">
        <property type="entry name" value="Glutamate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.285.10:FF:000003">
        <property type="entry name" value="Glutamate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000002">
        <property type="entry name" value="Glutamate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000030">
        <property type="entry name" value="Glutamate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.285.10">
        <property type="entry name" value="Glutamate Dehydrogenase, chain A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050724">
        <property type="entry name" value="Glu_Leu_Phe_Val_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006929">
        <property type="entry name" value="PRK09414.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43571">
        <property type="entry name" value="NADP-SPECIFIC GLUTAMATE DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43571:SF1">
        <property type="entry name" value="NADP-SPECIFIC GLUTAMATE DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="10">
        <location>
            <begin position="189"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="380"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="8">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW35575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="455" mass="48932" checksum="164A53321956D55B" modified="2011-11-16" version="1">MVLPYEPEFQQAYDELVSAVEDSTLFTEQPKYKKVIPVASVPERVIQFRVAWENDKGEIEVNNGYRVQFNSALGPYKGGLRFHPSVNLSILKFLGFEQIFKNALTGLSMGGGKGGSDFNPKGRTDSEIRRFCVAFMRQLARHIGPDTDVPAGDIGVGGREIGFLFGAYKQMRNHWAGVLTGKGLSWGGSLIRPEATGYGCVYYVEKMIEKATNGKETFAGKRVAISGSGNVAQYAALKVIELGGTVVSLSDSKGAIISKNGITKEQVAAIAAAKIQFKSLEEIIAESASVFSGENSVEYLAGVRPWTKVGQVDVALPSATQNEVSGDEAKALVEAGCKFIAEGSNMGSTKEAIEVFEANRASGVWYAPGKAANCGGVAVSGLEMAQNSARVQWTNEEVDAKLKDIMYTCFDNCYQTAIKYSTEKTEGTLPSLLKGANIAGFIKVADAMFDQGDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEK9</accession>
    <name>G3AEK9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_57838</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372183.1">
        <property type="nucleotide sequence ID" value="XM_007372121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEK9"/>
    <dbReference type="FunCoup" id="G3AEK9">
        <property type="interactions" value="745"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEK9"/>
    <dbReference type="GeneID" id="18872216"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57838"/>
    <dbReference type="eggNOG" id="KOG1414">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021530_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEK9"/>
    <dbReference type="OMA" id="HNAQTES"/>
    <dbReference type="OrthoDB" id="295274at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14687">
        <property type="entry name" value="bZIP_ATF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000053">
        <property type="entry name" value="BZIP transcription factor AtfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051027">
        <property type="entry name" value="bZIP_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021755">
        <property type="entry name" value="TF_Aft1_HRA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19304">
        <property type="entry name" value="CYCLIC-AMP RESPONSE ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11786">
        <property type="entry name" value="Aft1_HRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="352"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="115"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="214"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="292"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="312"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="328"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="346"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="556" mass="59419" checksum="9C1C85A0DDD1AE8A" modified="2011-11-16" version="1">MTESKSTFELEPNPFERSFATKESSASLDKMSDGAEASKVTSSSSTNKHNLHIPNISNLNSNGKLPALTPPIFTPGGRRLPPLLSPGAVNATSYSNPGTPGSALWNNFLANNNISQQQQQQQQQQQQHQQGQPQIGGTQNGYNSFLSSARKTGLTPNESNIRTGLTPGGLSHFPFGSQLAVPGLSTPGALLNGPITPGLSSLLGLTPGINGSHLSSMLPPVNGNSGASSQPPPQILQPQPVIHEEPYSVAPPPVVPQIPTSSSAPPLAPVNVPQQKEQPPADADDTPPRQTVEIKQENRKRSGDDLLDSPPSKKKSTRGKRPGTGKKQAKEEPDAKHSDSSSPNDSRNKSDDEKRKNFLERNRVAASKCRQRKKQLVQKMEEELAFYSNGYRELSAQVTQLHDQLLNLRGIIAGHKDCTLLAQSVGGFEAINQIVQQADLAIQMSAGGQTNMTSMPSTIPTNLHAQPQVVVQVAKDPIVRGSTTATPELYTNTTTPMVNDGNNNTTIASHHSLTDLRSTAAAQTMLLTSNGGMNNGDLRAINSMSNLAAMNQRQFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AET6</accession>
    <name>G3AET6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">21S rRNA pseudouridine(2819) synthase</fullName>
            <ecNumber evidence="7">5.4.99.43</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Pseudouridine synthase 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Pseudouridylate synthase PUS5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Uracil hydrolyase PUS5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_131996</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Pseudouridylate synthase responsible for the pseudouridine-2819 formation in mitochondrial 21S rRNA. May modulate the efficiency or the fidelity of the mitochondrial translation machinery.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>uridine(2819) in 21S rRNA = pseudouridine(2819) in 21S rRNA</text>
            <dbReference type="Rhea" id="RHEA:42556"/>
            <dbReference type="Rhea" id="RHEA-COMP:10113"/>
            <dbReference type="Rhea" id="RHEA-COMP:10114"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.43"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.43" evidence="7"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373178.1">
        <property type="nucleotide sequence ID" value="XM_007373116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AET6"/>
    <dbReference type="FunCoup" id="G3AET6">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AET6"/>
    <dbReference type="GeneID" id="18869628"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131996"/>
    <dbReference type="eggNOG" id="KOG1919">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_998077_0_0_1"/>
    <dbReference type="InParanoid" id="G3AET6"/>
    <dbReference type="OMA" id="CIITKIG"/>
    <dbReference type="OrthoDB" id="428658at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160143">
        <property type="term" value="F:21S rRNA pseudouridine(2819) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluA-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF81">
        <property type="entry name" value="21S RRNA PSEUDOURIDINE(2819) SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="12">
        <location>
            <begin position="12"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW35766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="279" mass="32204" checksum="AE0380AFEDA55396" modified="2011-11-16" version="1">MGKLDIVKHTFNYAIVNKSSNMICSGYQQSSLIPSLTQEFQQRNPEMTFDPAQFRICQRLDRFVTGGLIVARNRKWADKIRRVLGGKSQSMKLTRRYVGLIFGHDSLTSGVINYDIQALEKDYRGKPVSPRQLNTYPALTRFKLLTNLTRDPTPENILQYPDLFDNIQLMPIIFELHTGRKNQIRDHVIQAFKTPLLNDDQFIQFKMQSETGIFANSTKFIHNQIGLHSAILSMETNKGKEDFVFPIVAQKDRILWQGFVDDNGILLPEIIKELKLFTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEW3</accession>
    <name>G3AEW3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_48754</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373205.1">
        <property type="nucleotide sequence ID" value="XM_007373143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEW3"/>
    <dbReference type="STRING" id="619300.G3AEW3"/>
    <dbReference type="GeneID" id="18871260"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48754"/>
    <dbReference type="eggNOG" id="ENOG502QQID">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_714035_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEW3"/>
    <dbReference type="OrthoDB" id="299997at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="387" mass="43405" checksum="1013DFAB9CF3BC4A" modified="2011-11-16" version="1">MSKEPHPTAFNASFMPTTNQSRHLETTPPSLTKVNWKSFTSTPIKDPFTGEIYLTDIQSGTGSKEIPESAKTITAPLPDISFASEPQATPSQTFETILGKRSAAKNGAGKFQVPITPKSSTKKSKETSATFKALRPTINDVKPMQQRQEPPRVKPFSLVPESKNRRIDPRTSGLHTNFDLASLFSSGTSSNTVSVVYDYNEIESIRNQLIHTLVDNCKHANFANIYQLGQLRLADELSVCTGETEIFFEKLVYLFEDHILVWNHHDYLEEPRLIPVNNVKISSKESVVHILQTTDDYPYVTMLQSPSSAIIEKWVVALSDRNLVIPAEVITNTIKSVTNNLNKRDQNPSISSSIHDVSTMDSDIDSDQERIDKIIQGDMLMYDREFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF81</accession>
    <name>G3AF81_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_57962</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372282.1">
        <property type="nucleotide sequence ID" value="XM_007372220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF81"/>
    <dbReference type="STRING" id="619300.G3AF81"/>
    <dbReference type="GeneID" id="18872271"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57962"/>
    <dbReference type="eggNOG" id="ENOG502QUSB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027285_4_0_1"/>
    <dbReference type="InParanoid" id="G3AF81"/>
    <dbReference type="OMA" id="WADWEST"/>
    <dbReference type="OrthoDB" id="941679at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000111">
        <property type="entry name" value="Soluble cell wall protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050732">
        <property type="entry name" value="Beta-glucan_modifiers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631:SF14">
        <property type="entry name" value="FAMILY 17 GLUCOSIDASE SCW10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00587">
        <property type="entry name" value="GLYCOSYL_HYDROL_F17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442266" evidence="13">
        <location>
            <begin position="19"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="105"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="156"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="200"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="117"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="156"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="209"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="551" mass="56800" checksum="E9AF00763033AFC0" modified="2011-11-16" version="1">MNLSLLFIYLLVTNIAQGLPIQHQKRWSFFDLFDSQSSASAVATTLAPTPAVIPATTPAPVQAPVAVTTPVAVATPVQVVQLAATPSVAPQTTAAAPAAAAATASGTSDGSSWLANPTTTAATTQPQPEAAAAEDDADYPSETTITRILTVTRTGYSSDFPASNPTQASQPTTTTSSSSSSTGGGFLNWLAGLFGSSSSSSSSTQTTTVQPDASQVVSSDPSSFIEEPVSIPDPQPVSYPASSQVSIPSASASSINLSFQGGSPGKNALASSMATASAINGGSNAGAAAAVGSKGITYSPYTKTDQCKSASDVASDIAKLSNFDVIRLYSTDCSGIENVLSAMSSHQQLFLGVWNVDSVQGELQDIVTAISGSSRGWSAVHTIAIGNERVNAGAATVADIQNAVSQSRVFLKQNAPQYTGPIVSVDTLVAVVGNPALCEVSDYLAVNSHPYWDGGVQPENSGPWLQQQIANLQNVCGKDKQVLITETGWPTQGDTFGSCVPSVPNQVAAIKSIVNSLGSQVIMFTMYNDYWKDPGPYNVEQHWGIFGDPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFJ6</accession>
    <name>G3AFJ6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SEC14 homolog 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_58107</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(out)</text>
            <dbReference type="Rhea" id="RHEA:38691"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:38692"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Microsome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372396.1">
        <property type="nucleotide sequence ID" value="XM_007372334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFJ6"/>
    <dbReference type="FunCoup" id="G3AFJ6">
        <property type="interactions" value="137"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFJ6"/>
    <dbReference type="GeneID" id="18872325"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58107"/>
    <dbReference type="eggNOG" id="KOG1470">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014001_1_1_1"/>
    <dbReference type="InParanoid" id="G3AFJ6"/>
    <dbReference type="OMA" id="DHYTETI"/>
    <dbReference type="OrthoDB" id="75724at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071561">
        <property type="term" value="C:nucleus-vacuole junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902556">
        <property type="term" value="C:phosphatidylinositol transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043942">
        <property type="term" value="P:negative regulation of sexual sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol transfer protein PDR16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052578">
        <property type="entry name" value="PI_Transfer_CRAL-TRIO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824">
        <property type="entry name" value="GH16843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824:SF29">
        <property type="entry name" value="GH16843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2" id="KW-0492">Microsome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="6">
        <location>
            <begin position="165"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00083195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="374" mass="43231" checksum="826D3660B0407D83" modified="2011-11-16" version="1">MLSKLKLKSLTPTSSSSLKASDVRSTEQEVSGSVDSSINEKIDFESEKVFYPFEQPSVKSHIPQEPQLSAEQKEKYLAVLHHFQQEDLKVAISEDTHNHKKTELYQPLTHDEKSWLTRECFLRYLRATKWNEKDAINRVELTLAWRREFGIDKAMENQNKVNGETTSIENETGKEVILGYDNDSRPCLYLKPGRQNTKTSQRQVEHLVYSLERVIDYMPSGQDSLALLIDFKAHPVGTQGGKIPPVGVGRQVLHILQTHYPERLGKALLTNIPWLGWTFLKIIHPFIDPLTREKLVFDEPFEKYVPVEQLDVDFNGKVNFEYNHDKYWRRMIEISQEKKAKYMERFELFGGVVGLSEVDLRGDDSEIKHPVGTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFX6</accession>
    <name>G3AFX6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Malate dehydrogenase</fullName>
            <ecNumber evidence="3 12">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_58279</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="3 12"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372527.1">
        <property type="nucleotide sequence ID" value="XM_007372465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFX6"/>
    <dbReference type="FunCoup" id="G3AFX6">
        <property type="interactions" value="884"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFX6"/>
    <dbReference type="GeneID" id="18872390"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58279"/>
    <dbReference type="eggNOG" id="KOG1494">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047181_1_0_1"/>
    <dbReference type="InParanoid" id="G3AFX6"/>
    <dbReference type="OMA" id="ASCAEYI"/>
    <dbReference type="OrthoDB" id="4069699at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01337">
        <property type="entry name" value="MDH_glyoxysomal_mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000013">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000001">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001252">
        <property type="entry name" value="Malate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010097">
        <property type="entry name" value="Malate_DH_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01772">
        <property type="entry name" value="MDH_euk_gproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540">
        <property type="entry name" value="MALATE AND LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540:SF73">
        <property type="entry name" value="MALATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00068">
        <property type="entry name" value="MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="13">
        <location>
            <begin position="19"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="14">
        <location>
            <begin position="162"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="24"/>
            <end position="30"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="132"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW35115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="332" mass="34732" checksum="B847C9AED547B4FA" modified="2011-11-16" version="1">MLSKVAARSFSSSASAAYKVAVLGAGGGIGQPLSLLMKLNHKVTDLALYDIRGAPGVAADVSHIPTNSTVKGYNPDQLQEALTGADVIIVPAGVPRKPGMTRDDLFNTNASIVRDLAKAAADYAPKAALLIISNPVNSTVPIVAEVYKSKGVYDPKKLFGVTTLDVLRASRFVSEVAGTNPVNEKVTVVGGHSGITIIPLLSQTNHKDLDAETRDALVHRIQFGGDEVVKAKDGAGSATLSMAQAGARFAGAVLDGLAGEKDVIEPTFVDSPLFKSEGVEFFSSKVTLGVEGVKTVHPLGQLSEYEEELVKTAKETLIGNIKKGVDFVAQNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG21</accession>
    <name>G3AG21_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_48195</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372573.1">
        <property type="nucleotide sequence ID" value="XM_007372511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG21"/>
    <dbReference type="GeneID" id="18871192"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48195"/>
    <dbReference type="eggNOG" id="ENOG502RQCM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123970_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG21"/>
    <dbReference type="OMA" id="TWVKDWY"/>
    <dbReference type="OrthoDB" id="206335at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="134" mass="14997" checksum="421F747E56304460" modified="2011-11-16" version="1">MFSKSGTNTPIPGGVAHINKIQYHEQLDETITWIKDWYSPNLSNDLSDDAINDSVRLKGWYKTNINHKNGNSLAYNPDDVLNLNEWKYYTESTSMTDSNQSSSSDSSNGSAMDVDEVKQLGAAIRYEDDGLGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG27</accession>
    <name>G3AG27_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GDP-mannose transporter</fullName>
            <shortName evidence="5">GMT</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_58368</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the import of GDP-mannose from the cytoplasm into the Golgi lumen.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homooligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Golgi apparatus membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasmic vesicle membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TPT transporter family. SLC35D subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372578.1">
        <property type="nucleotide sequence ID" value="XM_007372516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG27"/>
    <dbReference type="FunCoup" id="G3AG27">
        <property type="interactions" value="546"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG27"/>
    <dbReference type="GeneID" id="18872417"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58368"/>
    <dbReference type="eggNOG" id="KOG1444">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025360_1_2_1"/>
    <dbReference type="InParanoid" id="G3AG27"/>
    <dbReference type="OMA" id="VWMLINC"/>
    <dbReference type="OrthoDB" id="417037at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00803">
        <property type="entry name" value="nst"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="334" mass="36911" checksum="FDFE13EF81C4CFE0" modified="2011-11-16" version="1">MEKSSSPQPGQSLASSISNSGPISILAYCLSSILMTVTNKYVLSGFSFNLNFFLLAVQSIVCIVTIGSLKSVGIITYRQFNKDEAKKWSPIAFLLVAMIYTGSKAIQYLSIPVYTIFKNLTIILIAYGEVLWFGGKVTSMALSSFLLMVFSSMIAYYGDNAAVRSQDDEFTLYLGYFWMLVNCFASASFVLIMRKRIKLTNFKDFDTMYYNNLLAIPILLISSLVFEDWSSENIAVNFPSDNRVTTISAMILSGVSSVGISYCSAWCVRVTSSTTYSMVGALNKLPIALTGLIFFDAVVNFWSVSSIFVGFIAGLVYAIAKQKQQREQQSLPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG36</accession>
    <name>G3AG36_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">tRNA uridine 5-carboxymethylaminomethyl modification enzyme C-terminal subdomain domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_131159</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the MSS1-MTO1 complex that catalyzes the 5-carboxymethylaminomethyluridine (cmnm(5)U) modification at the 34th wobble position (U34) of mitochondrial tRNAs.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MnmG family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372587.1">
        <property type="nucleotide sequence ID" value="XM_007372525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG36"/>
    <dbReference type="FunCoup" id="G3AG36">
        <property type="interactions" value="776"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG36"/>
    <dbReference type="GeneID" id="18869556"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131159"/>
    <dbReference type="eggNOG" id="KOG2311">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007831_2_2_1"/>
    <dbReference type="InParanoid" id="G3AG36"/>
    <dbReference type="OMA" id="CNPAMGG"/>
    <dbReference type="OrthoDB" id="3329at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070899">
        <property type="term" value="P:mitochondrial tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000145">
        <property type="entry name" value="tRNA uridine 5-carboxymethylaminomethyl modification enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.570:FF:000001">
        <property type="entry name" value="tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000002">
        <property type="entry name" value="tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.570">
        <property type="entry name" value="GidA associated domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049312">
        <property type="entry name" value="GIDA_C_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004416">
        <property type="entry name" value="MnmG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002218">
        <property type="entry name" value="MnmG-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020595">
        <property type="entry name" value="MnmG-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026904">
        <property type="entry name" value="MnmG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047001">
        <property type="entry name" value="MnmG_C_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044920">
        <property type="entry name" value="MnmG_C_subdom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040131">
        <property type="entry name" value="MnmG_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00136">
        <property type="entry name" value="mnmG_gidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806">
        <property type="entry name" value="GLUCOSE INHIBITED DIVISION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806:SF0">
        <property type="entry name" value="PROTEIN MTO1 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01134">
        <property type="entry name" value="GIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21680">
        <property type="entry name" value="GIDA_C_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13932">
        <property type="entry name" value="SAM_GIDA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01228">
        <property type="entry name" value="GIDA_assoc_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01280">
        <property type="entry name" value="GIDA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA uridine 5-carboxymethylaminomethyl modification enzyme C-terminal subdomain" evidence="7">
        <location>
            <begin position="590"/>
            <end position="663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="671" mass="74001" checksum="3B6974F777AB5A6E" modified="2011-11-16" version="1">MFRPTLKPRHISVIQKRSIILNADLQAKLAESAHHPVIVIGGGHAGCEAATGSARSGTSTTLITPHVDKIGTASCNPSMGGVGKGTLLREVDALDGVAARITDLAGIHFRILNSSRGAAVHGPRAQIDRKIYLNEMQKEILNYPNLNVLQASVEDIIIEPSVDQDSTGRKFGTVRGVILGDNTILACDKVVVTTGTFLGGEIHIGLKAYPSGRIGEAATFGLSKTFANAGFRLGRLKTGTPCRLSSKTIDYTHLLPQPSDFPPQPMSYMNDKVALQDQLVTCHQTNTTPEFHDLIRANLDKSIHIRETVKGPRYCPSIESKVIKFSDKSQHTVWLEPEGLDTDLVYPNGISCTMPAEIQEKLVKMMPGCENVTMVQPGYGVEYDYIDPRELKNTLETKLVDGLYLAGQINGTTGYEEAAAQGCIAGINAGLTQSGKQALDIKRSDGYIGVLIDDLITKGVEEPYRMFTSRSEFRISIRSDNADRRLTEMGYQAGVVGEKRYQHYTDEMKQFEQIKEHLQSVRISGAKLSPYVKNISTRPGFVVDAWKLLSYKDISFADFLPYLEQFNNGKLPSIYENLSDRLINRITVEGNYDPFKNAELKHLHAYNSDENLKLPENYTYTNVGNLKLSHEVCGILNTIQPKTIGQARRIQGVTPAAIFELYKLIKRTAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGB1</accession>
    <name>G3AGB1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_133020</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372662.1">
        <property type="nucleotide sequence ID" value="XM_007372600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGB1"/>
    <dbReference type="STRING" id="619300.G3AGB1"/>
    <dbReference type="GeneID" id="18869708"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133020"/>
    <dbReference type="eggNOG" id="ENOG502QUE5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014054_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGB1"/>
    <dbReference type="OMA" id="MEIDFTA"/>
    <dbReference type="OrthoDB" id="5374328at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018287">
        <property type="entry name" value="Hap4_TF_heteromerisation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF7">
        <property type="entry name" value="BZIP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10297">
        <property type="entry name" value="Hap4_Hap_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="4">
        <location>
            <begin position="96"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="162"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="330"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="442"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="492"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="538"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="16"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="164"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="232"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="330"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="492"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="544"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW35250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="613" mass="66333" checksum="E25DE559BEE75D84" modified="2011-11-16" version="1">MKTKGPSIAPRQQQIPTPMSMRPAMSPSSTASTPRQIVSTPPNTAASPPNSASPSANGSASLAPKGITQIITSKEWVLPPRPKPGRKPSVDTPASKRKAQNRAAQRAFRERRATRVQELEQKLMEVEKEKDLKEMTMINTINKLKVENQFLMKSLEQLKKEVGQIRGRQQHHQQQQQQQQPIQTRPPTINIKSHESSNHASPTNMGTSPASSNYSLQQISPAPSADSPPSSYNPRVNSISTLLPKNNTPQRPTDGNTVGTADYDCGICLKEECLCESVGLKIPGTNGSAVLEEQMSGFQPMPAVSLNRKRKATTEAREVDFTSLFSKKSEAKKMPEFKRRKDNADQAPSASLLHSTSESKPSFDENSPVENCGFCSDDTPCVCREAAEEAARLSNSLNQPQQEISETLPPIQISNSNNIRKSALPVMHPGPSVELREMTNLKSTGNAAPPVTVTSSTSSKYELESDSGCTGNPGTCRQCQTDPMSTLFCTTIASRGSNSSPSGQTPGRSMSVNSLTNPSTPIEPLLAAANAAHAEVANNPEPITPSSSKNSNGEQPSGIFIPVGDAYKTLSRHKQFSSVDFGTLVGKLTTRGMQVEVQSVVNVLRELDKRVYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGF1</accession>
    <name>G3AGF1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Acyl carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_58508</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372702.1">
        <property type="nucleotide sequence ID" value="XM_007372640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGF1"/>
    <dbReference type="STRING" id="619300.G3AGF1"/>
    <dbReference type="GeneID" id="18872476"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58508"/>
    <dbReference type="eggNOG" id="KOG1748">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108696_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGF1"/>
    <dbReference type="OMA" id="RFKTPRD"/>
    <dbReference type="OrthoDB" id="448946at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099128">
        <property type="term" value="C:mitochondrial [2Fe-2S] assembly complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000035">
        <property type="term" value="F:acyl binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.10:FF:000003">
        <property type="entry name" value="Acyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01217">
        <property type="entry name" value="Acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00517">
        <property type="entry name" value="acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002148">
        <property type="entry name" value="PRK00982.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="ACYL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863:SF28">
        <property type="entry name" value="ACYL CARRIER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13 14" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 14" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Carrier" evidence="15">
        <location>
            <begin position="48"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW35290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="129" mass="14491" checksum="C3FFD581FC49A84C" modified="2011-11-16" version="1">MFRNTVLRSLRAPVLARVQPIVRPSLIRPMPTITMIRSYAAFPALTRELAKERILELLEGYDKTILPKEGDISEDASFVNDLGLDSLDVVEIVIELEHEFNIQIPDNEADSLKTVGQTIDFIMAQPDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGL0</accession>
    <name>G3AGL0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_58576</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372761.1">
        <property type="nucleotide sequence ID" value="XM_007372699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGL0"/>
    <dbReference type="FunCoup" id="G3AGL0">
        <property type="interactions" value="851"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGL0"/>
    <dbReference type="GeneID" id="18872511"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58576"/>
    <dbReference type="eggNOG" id="KOG0761">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGL0"/>
    <dbReference type="OMA" id="YWWGYES"/>
    <dbReference type="OrthoDB" id="1747031at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990542">
        <property type="term" value="P:mitochondrial transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031921">
        <property type="term" value="P:pyridoxal phosphate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045315">
        <property type="entry name" value="Mtm1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45760">
        <property type="entry name" value="FI19922P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45760:SF2">
        <property type="entry name" value="FI19922P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 13" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="36"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="183"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="293"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW35349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="392" mass="43434" checksum="3289C82892B3C04B" modified="2011-11-16" version="1">MSLSSELAAGSMEEHDLKFGEKFVAKPKVAIPENDISITQRMISACSGSLITSLVVTPFDVIRIRIQQQEILPQDTCCHNSLKVESVPATGRNTLVKHMASPSAMAKAAEATPELFWMHNRYCKAENCSRITSTFQGFACVAKNEGVGTLWRGLSLTLFMAIPSNIIYFTGYEYIRDHSPISNHPLNPLLCGAFARIMSATFIAPAELIKTRLQSIPSDSKTSSKVLNNLLKDSFSLVRQNGAGTLFKGLQITLWRDVPFSGIYWSCYELFKSRIGHALNADFDNSRGTNDWKVFATSFFSGSISGTVAAFFTNPFDVGKTRLQITFEEGQKPGGYNRNMFKFLINIYRREGIGALYAGFGPRVMKIAPACAIMISSYEIGKKFFKDGNNHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGS8</accession>
    <name>G3AGS8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HRQ family protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_146625</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372823.1">
        <property type="nucleotide sequence ID" value="XM_007372761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGS8"/>
    <dbReference type="GeneID" id="18870723"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_146625"/>
    <dbReference type="eggNOG" id="ENOG502RZ1N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025462_2_0_1"/>
    <dbReference type="InParanoid" id="G3AGS8"/>
    <dbReference type="OMA" id="TYRYPIQ"/>
    <dbReference type="OrthoDB" id="5043642at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021848">
        <property type="entry name" value="HODM_asu-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11927">
        <property type="entry name" value="HODM_asu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="346" mass="40116" checksum="16943F371E4EE157" modified="2011-11-16" version="1">MLLLFVLLLGLGLGFYWYKHPKNKIQAVPHNFQWHTEKPLPIRPFVNKRNFNPSIGIKNISDQPENWLLIENTYLSNITIRRKHLEANPHQTMQIPDNERTVAALCEFYNLVTGFLTARYPKLFLHGWTGKLHNEITNDDIPFKADPSNPREMCNALGRTIEEDFLILMKDNPDNKEEEYILRAAVNAFPAGFDPSKSYNKPISAIHGPVPQYASRLAFSMHKFFNNLKQGDIWVRHNWSIQTHNQHFNLDSNHGRPGQEVKKIGFDEIDFEQGCFLRVERQAFLRLPNSGAIVMTVRTYLTPMPQIKKEGLGPELCRAIDSLPEDLAYYKKRDAWGEAVKQYLMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGY8</accession>
    <name>G3AGY8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aldehyde dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ALD7</name>
        <name evidence="10" type="ORF">SPAPADRAFT_57711</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 7">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007372011.1"/>
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        <property type="interactions" value="456"/>
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    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57711"/>
    <dbReference type="eggNOG" id="KOG2456">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_005391_3_1_1"/>
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    <dbReference type="OMA" id="MKDQKVP"/>
    <dbReference type="OrthoDB" id="440325at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018484">
        <property type="term" value="F:4-hydroxybenzaldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047770">
        <property type="term" value="F:carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046185">
        <property type="term" value="P:aldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000025">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF16">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE TYPE III, ISOFORM Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036492">
        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="17"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR036492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="445" mass="49251" checksum="4DB2E8E55C248E7C" modified="2011-11-16" version="1">MESLHTWAKPEKVTDLPINLKTQPVYIERIPYGVVLIISPFNYPFFLSISAVVGAIAAGNAVVLKQSEMTPRFSKLFSEILTKSLDPDIFLAVNGGIPETTELLNQKFDKIMYTGNNMVGTIVAKKAAETLTPTILELGGKSPAFVLDDVQEKDLDTVARRIAWGRFTNAGQTCVAVDYVLVPKKLHDKFITSVKKVVSEQLYPKINANDDEFTHIIHDRAFKNLTGLLKTTKGEIVVGGDSDAKSRYIAPTVVDNVDWDDSVMKNEIFGPILPIITYEKLSDALIEVQRRHDTPLAQYIFTSGSTSRKYNRQVDQILTAIRSGGTIINDVLLHVALLNAPFGGVGNSGMGAYHGKFSFRHFTHERTTIESKLWNEAMIKVRYPPFNDTKNTLLQVTQTSYGGNVWFNRTGDVRVAGPSTLFSVWNGFIGTLGILGTFVSKASKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH43</accession>
    <name>G3AH43_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58702</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372885.1">
        <property type="nucleotide sequence ID" value="XM_007372823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH43"/>
    <dbReference type="FunCoup" id="G3AH43">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AH43"/>
    <dbReference type="GeneID" id="18872568"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58702"/>
    <dbReference type="eggNOG" id="ENOG502S5QU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028690_2_0_1"/>
    <dbReference type="InParanoid" id="G3AH43"/>
    <dbReference type="OMA" id="NRNRMWA"/>
    <dbReference type="OrthoDB" id="3257538at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019171">
        <property type="term" value="F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052741">
        <property type="entry name" value="Mitochondrial_HTD2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28152">
        <property type="entry name" value="HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28152:SF1">
        <property type="entry name" value="HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="295" mass="33953" checksum="240517202264127A" modified="2011-11-16" version="1">MSLITKECIYKWANFVKANPTILKDTIAIGPTTHLVNLLNCILGKPTPATPVLAPYKYFNQGFHFLFNNQLNLKLDSDGYDTYQAPTNEKGEQLYLRRLWARGEIQFPNQYPKINTPITCKESIKSVRIIEESTLVTIVREFQDGNKLNSLLTENRTLSYTNELYTPGEPVKLISQVNETDKHIQISPIDLLKYSMLTYNLHKIHINPNYCRSIEDLPTTIVHGPFQVTLLLYWFALNHPGITPVNFKYRTYEPCFVNDEISLQIVKNSDDHFVLSLYDKNSSKVYIDGTLLTKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH72</accession>
    <name>G3AH72_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58741</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372914.1">
        <property type="nucleotide sequence ID" value="XM_007372852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH72"/>
    <dbReference type="GeneID" id="18872581"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58741"/>
    <dbReference type="HOGENOM" id="CLU_2887259_0_0_1"/>
    <dbReference type="InParanoid" id="G3AH72"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="63" mass="7313" checksum="2385C576AF07EAEB" modified="2011-11-16" version="1">MVPEDESNSQVHSIQEPSSSSIRTRVRRRVSKSLKKLNLVVHRDKEFVVTKELLELKAKVLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHA6</accession>
    <name>G3AHA6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58761</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372948.1">
        <property type="nucleotide sequence ID" value="XM_007372886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHA6"/>
    <dbReference type="GeneID" id="18872595"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58761"/>
    <dbReference type="eggNOG" id="ENOG502RQDY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169796_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHA6"/>
    <dbReference type="OMA" id="KWPIANT"/>
    <dbReference type="OrthoDB" id="4010196at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="110" mass="12935" checksum="76C8944CBEC18DF7" modified="2011-11-16" version="1">MNRILSTRTTTLPRTIRNYSVRIPQQESIPQNKPRLSQFTTAMTHTFLFAGIAYMGLQAIWLHLEYEQAEGDLTAKSKSLEEKIQSIVDSKKEELIQVQQTNSKRWYKFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHF3</accession>
    <name>G3AHF3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2',3'-cyclic-nucleotide 3'-phosphodiesterase</fullName>
            <ecNumber evidence="3">3.1.4.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_134037</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the metabolism of ADP-ribose 1',2'-cyclic phosphate which is produced as a consequence of tRNA splicing.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 2H phosphoesterase superfamily. CPD1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.37" evidence="3"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373701.1">
        <property type="nucleotide sequence ID" value="XM_007373639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHF3"/>
    <dbReference type="FunCoup" id="G3AHF3">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHF3"/>
    <dbReference type="GeneID" id="18869777"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134037"/>
    <dbReference type="eggNOG" id="ENOG502RY6J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088289_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHF3"/>
    <dbReference type="OMA" id="FEPHITI"/>
    <dbReference type="OrthoDB" id="514292at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009187">
        <property type="term" value="P:cyclic nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012386">
        <property type="entry name" value="Cyclic-nucl_3Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28141">
        <property type="entry name" value="2',3'-CYCLIC-NUCLEOTIDE 3'-PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28141:SF1">
        <property type="entry name" value="2',3'-CYCLIC-NUCLEOTIDE 3'-PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07823">
        <property type="entry name" value="CPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="111"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="111"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="125"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="243" mass="27389" checksum="8C308FB8ED3B43F5" modified="2011-11-16" version="1">MGSLNTLFPGQPPRFEPHITITTNIMIDLDDPNKTRDDVDRILSASAVALSSLPKNHDKLVRLGRVNSQRKFFKKLYFQVDSDPNLVSFARIIRELFVILPHDIEEENIKQNPQLYTKDSNGNTVKRKLSKKRTKSQSPPAQEAKQFDTSGLQYLAAQKAADWSLNEFDPHLSLVYSNIHPIDNALWRTIKTRISDYLDIDNVDSDSFNSGVSGLGWDGGVLKLVLCEGDVNDWIVLGSVDVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHI4</accession>
    <name>G3AHI4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor IIB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">General transcription factor TFIIB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_59562</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373732.1">
        <property type="nucleotide sequence ID" value="XM_007373670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHI4"/>
    <dbReference type="FunCoup" id="G3AHI4">
        <property type="interactions" value="828"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHI4"/>
    <dbReference type="GeneID" id="18872973"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59562"/>
    <dbReference type="eggNOG" id="KOG1597">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043736_1_0_1"/>
    <dbReference type="InParanoid" id="G3AHI4"/>
    <dbReference type="OMA" id="LYMACRM"/>
    <dbReference type="OrthoDB" id="25790at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000008">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000036">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="16">
        <location>
            <begin position="17"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW34148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="342" mass="37823" checksum="FB046D50FAD6AC4C" modified="2011-11-16" version="1">MSTNTINSTYTGPNLNVTLVCPDCRQFPPDLVERFSEGDIICGNCGLVLSDRIVDTRSEWRTFNNDDQNGDDPSRVGDSGNPLLDTEDLSTMISYVPQGGNRVGRDLTRIQQKSLVDKKNNALNAAYIRIQELCNGYQLPKTVTDAAKHLYKAVREDKLLRGKSQDSIMAAVILLGCRRSDVPRTLEEIRALTNVPSKQIAKVARLIKKIIHSHLVANPQSSILEETTSLTTHSAEDLIRRFCSHLGLTPQVTNAAEHIARQCKDIGVLAGRSPTTIAAAAIYLAGKAFDVELTQQQIRDKTGVSIGTIKTSYKVMYENKDKLLDPYWIESGKVKLDNIPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHI5</accession>
    <name>G3AHI5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_54334</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Transports C5-C7 oxodicarboxylates across the inner membranes of mitochondria. Can transport 2-oxoadipate, 2-oxoglutarate, adipate, glutarate, 2-oxopimelate, oxaloacetate, citrate and malate. The main physiological role is probably to supply 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol where they are used in the biosynthesis of lysine and glutamate, respectively, and in lysine catabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373733.1">
        <property type="nucleotide sequence ID" value="XM_007373671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHI5"/>
    <dbReference type="FunCoup" id="G3AHI5">
        <property type="interactions" value="237"/>
    </dbReference>
    <dbReference type="GeneID" id="18871854"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54334"/>
    <dbReference type="eggNOG" id="KOG0754">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_5_2_1"/>
    <dbReference type="InParanoid" id="G3AHI5"/>
    <dbReference type="OMA" id="LPFQYQF"/>
    <dbReference type="OrthoDB" id="434783at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005310">
        <property type="term" value="F:dicarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006839">
        <property type="term" value="P:mitochondrial transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000034">
        <property type="entry name" value="Mitochondrial 2-oxodicarboxylate carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051752">
        <property type="entry name" value="Mito_2-oxodicarb_carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356:SF1">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="262"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="10"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="105"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="198"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW34149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="290" mass="32012" checksum="E75C0D1BDD5C448A" modified="2011-11-16" version="1">MSAPAEKQPLPFFYNFLAGAIAGVSEIIVMYPLDVIKTRQQLDSTGQYKGTLDCLKKIVKEEGFSRLYKGIAAPILMEAPKRATKFAANDEWGKFYRKQFEVKTMTQPLAVLTGATAGATESLVVVPFELVKIRLQDKTTKFNGMGEVVKHIVKENGLLGLYKGTESTAWRHIWWNAGYFGCIHQLRSLFPKPKDATEKTLIDLTCGAIGGTVGTILNTPFDVVKSRIQAGSTKYKWTYPSLAIVAKEEGFGALYKGFIPKVLRLGPGGGILLVVFTACMDFFRTVHEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHJ9</accession>
    <name>G3AHJ9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_54347</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373747.1">
        <property type="nucleotide sequence ID" value="XM_007373685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHJ9"/>
    <dbReference type="STRING" id="619300.G3AHJ9"/>
    <dbReference type="GeneID" id="18871856"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54347"/>
    <dbReference type="eggNOG" id="ENOG502S53E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1971865_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHJ9"/>
    <dbReference type="OMA" id="KKSLQHM"/>
    <dbReference type="OrthoDB" id="5239630at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019034">
        <property type="entry name" value="UPF0390"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09495">
        <property type="entry name" value="DUF2462"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="127" mass="14431" checksum="533BFFDEE575E2F7" modified="2011-11-16" version="1">MAQGKIKLKAKAPARVTKKQQNPKRNAPKIIKPKKTVAKEAKKLTKVHQSQLMTSTEKLIASRVGHLELIKGSRREIEKEQKAADKNNAANKEKLEPTLAIPELTALDPDLVLEKEKKERKSRRRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHX3</accession>
    <name>G3AHX3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59703</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373871.1">
        <property type="nucleotide sequence ID" value="XM_007373809.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHX3"/>
    <dbReference type="GeneID" id="18873038"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59703"/>
    <dbReference type="eggNOG" id="ENOG502RQF4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1511509_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHX3"/>
    <dbReference type="OrthoDB" id="4026234at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="178" mass="20242" checksum="5A0D5D123E40D214" modified="2011-11-16" version="1">MPSLTLGSSFEDSLQQALKKQKTSQFSSIPTSTQDYTAHKHHHKHHHHHHGHHNHHNHYIDDNMTQEKQAQYHHHHHHHQLHKTTDAVVDATHGTITPISHAQSLDSVMSDIAMSDEELTRQVSPGDLELITEKHFILKPDIYECPCGHFHGPGQDAHHDHDIHSVGLSDHPLLQGPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHX9</accession>
    <name>G3AHX9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein N-terminal and lysine N-methyltransferase EFM7</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Elongation factor methyltransferase 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">EFM7</name>
        <name evidence="7" type="ORF">SPAPADRAFT_59716</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">S-adenosyl-L-methionine-dependent protein methyltransferase that trimethylates the N-terminal glycine 'Gly-2' of elongation factor 1-alpha, before also catalyzing the mono- and dimethylation of 'Lys-3'.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. EFM7 family.</text>
    </comment>
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    <dbReference type="EMBL" id="GL996500">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007373815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHX9"/>
    <dbReference type="FunCoup" id="G3AHX9">
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    </dbReference>
    <dbReference type="STRING" id="619300.G3AHX9"/>
    <dbReference type="GeneID" id="18873042"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59716"/>
    <dbReference type="eggNOG" id="KOG2920">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="InParanoid" id="G3AHX9"/>
    <dbReference type="OMA" id="VGHNPLW"/>
    <dbReference type="OrthoDB" id="46564at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:N-terminal protein N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03223">
        <property type="entry name" value="Methyltr_EFM7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EFM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF10">
        <property type="entry name" value="PROTEIN N-TERMINAL AND LYSINE N-METHYLTRANSFERASE EFM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51560">
        <property type="entry name" value="SAM_MT_NNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="87"/>
            <end position="89"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
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        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="260" mass="30063" checksum="869FD8D7FD38B6B0" modified="2011-11-16" version="1">MSDDEVSFDGNLFEEPEDFLPPPPESHFAKYTRKVVHDNQPQEITLKLVGKSPLWGHLLWNAGIYTADYLDKHAEDLVKGKKILELGAAAGLPSLICSLNQASEVVCTDYPDADLISHIDYNFKDLESKTKLCDYRVQGYIWGQDVSPLVYNKEEVVNIKEEDKFDLIILSDLVFNHTEHHKLLATCRKSLKSNGKCLVVFSPHRAWLLEEDLKFFVTCEEYDLKAEKIELVNWKPMFEEDEESAEIRSRVYSYFLNPQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI26</accession>
    <name>G3AI26_SPAPN</name>
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        <recommendedName>
            <fullName evidence="2">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_54491</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007373862.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="619300.G3AI26"/>
    <dbReference type="GeneID" id="18871869"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54491"/>
    <dbReference type="eggNOG" id="KOG0154">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021974_1_0_1"/>
    <dbReference type="InParanoid" id="G3AI26"/>
    <dbReference type="OMA" id="PVFMRID"/>
    <dbReference type="OrthoDB" id="21470at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051189">
        <property type="entry name" value="Splicing_assoc_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14195">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="567"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
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        <location>
            <begin position="528"/>
            <end position="550"/>
        </location>
    </feature>
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            <end position="96"/>
        </location>
    </feature>
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        <location>
            <begin position="155"/>
            <end position="188"/>
        </location>
    </feature>
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        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <begin position="209"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="613" mass="70608" checksum="0E38EB965BAB81BC" modified="2011-11-16" version="1">MMQVVEYTNSHHDERMSKQLRKSPVLFVKAKEVYDPNSILHELARKNKTLLEGVESINLEHDEESSQENDDYFDEDAVEFEDDEDIVEDDHNDENYLEASMQKWQNNMRHESPEPIRSSGHQETPEELGEQLQPHSDNIAGRSFQSLPSGHAPEEVDSPTEENDSSLEEFNEVESDSPMEQELEEPAQEPEKANKAEPEEPPVQTQSLDIIIDTPTSTTIIKPEPVQNTEESTPEPEQEPEYGFLPEDYEFDVSRIEVQNVRFGIHNQYYVKCLELTGSDFAWIDEEEVIEFALAHGVKEHRLDKFLSFVTNGMIDQEEEESEPEVYISDDSESEDEDEEEEQEVDQEDDLESLIQYSKSSSQGFLAFGDRDFSNNTPAKYRSTFENLDIDDDLQESLHRQLANYKQNKKHKLNQKQKQLQEEAIMNNDLLIKYPDALRIKDIRYEFESLLKDENRTSVSFPKLDSHGHQTIKKLAKYYNMGVTNCGQSTNKYLKISKNKTTYKYYPNYDQIDRILRGRPIFHRQDVKRTPKTDKAEKAKKVTRGADSKARVREGDIVGAEAPEIDQSNVGRQMLEKLGWVKGQGLGAHGNQGINEPIVAKVKMSKTGIKETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3AID2</accession>
    <name>G3AID2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_49446</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373986.1">
        <property type="nucleotide sequence ID" value="XM_007373924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AID2"/>
    <dbReference type="GeneID" id="18871338"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49446"/>
    <dbReference type="HOGENOM" id="CLU_1403238_0_0_1"/>
    <dbReference type="InParanoid" id="G3AID2"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442427" evidence="1">
        <location>
            <begin position="20"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="194" mass="18306" checksum="BE410FC83B67FA56" modified="2011-11-16" version="1">MLFKNIALSTALLASAVMADDGDTTIITTVTVTQTVYTPEESSSMAAASAASEASVASAASAASVSSVASVASVASASSASAASAAAAASSSLASVASAASAASVASAKAAKQTALARYNGIVAVGSNVSNKTNTTITTSASTVPIHTTAYTNHTTSTPPPAATTNKVTSTGGAFSNSVGVGAAGLLAIVAALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIF2</accession>
    <name>G3AIF2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_70535</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374006.1">
        <property type="nucleotide sequence ID" value="XM_007373944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIF2"/>
    <dbReference type="STRING" id="619300.G3AIF2"/>
    <dbReference type="GeneID" id="18875331"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70535"/>
    <dbReference type="eggNOG" id="KOG2241">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009710_6_4_1"/>
    <dbReference type="InParanoid" id="G3AIF2"/>
    <dbReference type="OMA" id="KKCKLRG"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF33">
        <property type="entry name" value="AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="4">
        <location>
            <begin position="19"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="193" mass="21850" checksum="5E3816209D2D0B89" modified="2011-11-16" version="1">MIRSILTKRAYSVAPNTFHPQYLKLQVGQIQSCKRHEASDKLYISQIAIGENNGEKSTLQVCSGLVPFLPIEQMYNKKVVVVTNMKPRKLRGEASMGMILAAEAEVNEKLQVEVVEPPTGSAIGERLYFGSENHEFQPPKLKDKVWEYIQPRLRTNDQKQIVFIDEDNVHHILRGQDPNESATVPTLTNSLVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIH7</accession>
    <name>G3AIH7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_59875</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374031.1">
        <property type="nucleotide sequence ID" value="XM_007373969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIH7"/>
    <dbReference type="STRING" id="619300.G3AIH7"/>
    <dbReference type="GeneID" id="18873114"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59875"/>
    <dbReference type="eggNOG" id="KOG1502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_9_2_1"/>
    <dbReference type="InParanoid" id="G3AIH7"/>
    <dbReference type="OMA" id="IASCMNE"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="20"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="347" mass="38168" checksum="1E93D1866A99D05D" modified="2011-11-16" version="1">MSATASTTANTAATGTNATVFVAGATGFIGKHVVKQLLEQNYYVVGSVRSKEKGDALTVLLANPNFNYEIVEEIEEKGAFDLALKKFPNVSVFIDAATPLPIGVNYDKQLIKRSSKGIEHILHSIKEYGVNVRRVVMTSSIAACMNFEQANNPAYVVDPTKWNPDSHHAGKDTPVTAYFYAKAAAEKAAWDFVQKKKCNFTLTTILPGYTFGPHAFDTNVSDVLNTSTEIINKIAGLSPNDYIPNFGGLFVDVRDVARAHILAFEKPPAAEKRLILVSEEFSAQTIVNILHKFYPALQLPVGEPNKPLLGLKFNNEETRKILAFELVPLETSIKDTFDQIFRARGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIQ6</accession>
    <name>G3AIQ6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_59894</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. TatD-type hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374057.1">
        <property type="nucleotide sequence ID" value="XM_007373995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIQ6"/>
    <dbReference type="FunCoup" id="G3AIQ6">
        <property type="interactions" value="603"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIQ6"/>
    <dbReference type="GeneID" id="18873125"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59894"/>
    <dbReference type="eggNOG" id="KOG3020">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031506_1_0_1"/>
    <dbReference type="InParanoid" id="G3AIQ6"/>
    <dbReference type="OMA" id="CSDIFFE"/>
    <dbReference type="OrthoDB" id="787137at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050891">
        <property type="entry name" value="TatD-type_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10060:SF15">
        <property type="entry name" value="DEOXYRIBONUCLEASE TATDN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10060">
        <property type="entry name" value="TATD FAMILY DEOXYRIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01090">
        <property type="entry name" value="TATD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01091">
        <property type="entry name" value="TATD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="391" mass="44638" checksum="25E3BFDD7DA5602B" modified="2011-11-16" version="1">MSTWRPRYYDIGVNFSDGMFQGTYNGHSKHPCDINQVIDRAHLFNVDKMLITASNIQESQNHFTLCEKYANNFDSTAGVHPCTVAEEFYIKDNDEYTNTLRDDVDEKLTRLKNIAEEGHKLGHVKAFGEIGLDYDRLHYSNKVQQCEMFKKQLEVHAQLKHLDLPLFLHMRDACDDFVSIIKPFIEAGSIRKGNGVVHSFTGTEEELNKLLGLGFYIGINGCSLKTEENVQVASKIPIDRLLIETDAPWCEIRKSHASYKYISSYPNKFYPDIKTTVPEEPQEVQDSKKSKKNGGPMFKLAENLPFPAIKKEHYAKHRQFVQSSLENLPNADIDTKVGMYAHPMIVSRNEPVNVGHVAEILCTIHGIKQEKEIESFIDLIFENSCKLFKTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ58</accession>
    <name>G3AJ58_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Band 7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_59177</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373399.1">
        <property type="nucleotide sequence ID" value="XM_007373337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ58"/>
    <dbReference type="STRING" id="619300.G3AJ58"/>
    <dbReference type="GeneID" id="18872808"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59177"/>
    <dbReference type="eggNOG" id="KOG2621">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024949_4_1_1"/>
    <dbReference type="InParanoid" id="G3AJ58"/>
    <dbReference type="OMA" id="IQQMVRV"/>
    <dbReference type="OrthoDB" id="2105077at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13437">
        <property type="entry name" value="SPFH_alloslipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000004">
        <property type="entry name" value="Putative membrane protease family, stomatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043202">
        <property type="entry name" value="Band-7_stomatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264:SF19">
        <property type="entry name" value="AT06885P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264">
        <property type="entry name" value="BAND 7 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="2">
        <location>
            <begin position="8"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="285" mass="31631" checksum="3292A54627559EAC" modified="2011-11-16" version="1">MFCFLCENPYREVDQGEVGLVQTFGRLTRTVEPGLSYVNTWSEKLTRVSIKINIREIPAQKCFTKDNVSVIITSVVYYNIIDPQKAIYSIADIHSAIVERTQTTLRDVIGGRTLQDVVEKREEIAESIEQIIAKTAFDWGVNIESILIKDLTLPDKVQSSLSMAAEAKRIGEGKIINAKAEVESAKLMRKAADILASKPAMQIRYLDALQNMAKSPGTRVIFMPSAQEVERMATANIAPSVPSGKDKPFDLDEDTDWAGQEAVSANVTGNRRIIDTVAIQEAMQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ93</accession>
    <name>G3AJ93_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="7">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_48957</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="7">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="7"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373434.1">
        <property type="nucleotide sequence ID" value="XM_007373372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ93"/>
    <dbReference type="FunCoup" id="G3AJ93">
        <property type="interactions" value="899"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ93"/>
    <dbReference type="GeneID" id="18871289"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48957"/>
    <dbReference type="eggNOG" id="KOG0350">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_15_2_1"/>
    <dbReference type="InParanoid" id="G3AJ93"/>
    <dbReference type="OMA" id="HLEWLVI"/>
    <dbReference type="OrthoDB" id="3370at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17956">
        <property type="entry name" value="DEADc_DDX51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="227"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="446"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="36"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="621" mass="69689" checksum="D0AA84777782CBB2" modified="2011-11-16" version="1">MFGARFDPSALESDVVDVTPRRNLLIRKRKISEVVDKEEDSEEEQDSEESSSEEEQAEQELSDADAGSDSEPEPSEQDSESEQEEDDNMSIDEEEAGSDKDEEEVQQDKDYVSKHQSIFSKFKKSIQVESTETEEITDDPIEVQDLAPLPQPALPKDRKLVSTTTHLKSLNWLATPIYASPSDTKPFTEFPLSQFMQRNLTSNNFTNAFSVQIAVLNLMLQDIHDHKLQPDVKGDILVNASTGSGKTLAYSIPIIESLHTRVVPRIRAIILVPTKPLINQVRSTLLVLSKGTNLSVVSLKNDISIKDEASKLIENVPDIIVSTPGRLVEHILQSSIDLSALQFLVIDEADRLLNQSFQNWCQILLSKIDNNINIAEEWKLPVQKLIFSATLTTDAGKLSSLQFQKPRLVIVNDEKELVNEIFTVPPTLSEFKLQFGSAKSSIKPLILTKFLISQGKLSNVLIFTKSNESSLRLSKLLQLLFSQLNKNVNIAYMNSTNNRSSIRTKILKEFSNQTINILVATDLIARGIDLVTITDIVNYDLPNSSREYVHRVGRTARANNIGQAYSLCFGKGEIKWFDKISKDVGRKRDVEAIDASVKELINKEDEDAYANVLQELKSQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJD3</accession>
    <name>G3AJD3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_134963</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374176.1">
        <property type="nucleotide sequence ID" value="XM_007374114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJD3"/>
    <dbReference type="STRING" id="619300.G3AJD3"/>
    <dbReference type="GeneID" id="18869856"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134963"/>
    <dbReference type="eggNOG" id="KOG0157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_27_0_1"/>
    <dbReference type="InParanoid" id="G3AJD3"/>
    <dbReference type="OMA" id="ISPMYMG"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11063">
        <property type="entry name" value="CYP52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002974">
        <property type="entry name" value="Cyt_P450_E_CYP52_ascomycetes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047146">
        <property type="entry name" value="Cyt_P450_E_CYP52_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002402">
        <property type="entry name" value="Cyt_P450_E_grp-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287:SF1">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00464">
        <property type="entry name" value="EP450II"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01239">
        <property type="entry name" value="EP450IICYP52"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602402-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="519" mass="60106" checksum="56DDEA9473A8F96F" modified="2011-11-16" version="1">MDPLDYLTKWYVVTTVGVILYSVYQYIHTKQLMKRLGAKPFTNDEGDGWFGFYVWKQLLDKKAEGTLVDFGHERYEKITNPNCETFQFRIFGKPLISTKDPENIKAILATQFNDFSLGRRHEIFQPLLGDGIFTLDGEGWKHSRAMLRPQFVREQVGHVKLLEPHLQVFFKHIRKNKGKVFDLQELFFRLTIDSATEFLFGESVQSLRDESIGMQLEATQIDGKQEFATAFNFSQNFLASRSVLQELYWLLNTKKFRDCNAKVHKFASYYVEKALNLTEKELQEEKGYVFLYELAKQTKDPVILRDQLLNILVAGRDTTAGLLSFMFFELARNPDVFEKLKEEIYTKFGSGDDVQFNEITFETLKQCEYMKAVINECLRLYPSVPQNFRIATRDTTLPHGGGPDGMSPVLVRKGQNIVYIISSTHKTEKYYGKDAADFNPQRWFEPETKKLGWAFLPFNGGPRICLGQQFALTEASYVTTRLIQEFPNLTLGDPDTRYPPLKMSHLTMSLFEGCNVKMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJD8</accession>
    <name>G3AJD8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16">E3 ubiquitin-protein ligase listerin</fullName>
            <ecNumber evidence="5 16">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">RING-type E3 ubiquitin transferase listerin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_60032</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">E3 ubiquitin-protein ligase component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. Mediates ubiquitination of proteins derived from mRNAs lacking stop codons (non-stop proteins) and other translation arrest products induced by poly-lysine sequences and tandem rare codons. Ubiquitination leads to CDC48 recruitment for extraction and degradation of the incomplete translation product. May indirectly play a role in chromatin function and transcription.</text>
    </comment>
    <comment type="function">
        <text evidence="16">E3 ubiquitin-protein ligase. Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the ribosome quality control complex (RQC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the LTN1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5 16"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374181.1">
        <property type="nucleotide sequence ID" value="XM_007374119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJD8"/>
    <dbReference type="STRING" id="619300.G3AJD8"/>
    <dbReference type="GeneID" id="18873187"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60032"/>
    <dbReference type="eggNOG" id="KOG0803">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015885_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJD8"/>
    <dbReference type="OMA" id="DISKEGM"/>
    <dbReference type="OrthoDB" id="6108at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16491">
        <property type="entry name" value="RING-CH-C4HC3_LTN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000038">
        <property type="entry name" value="E3 ubiquitin-protein ligase listerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039795">
        <property type="entry name" value="LTN1/Rkr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054477">
        <property type="entry name" value="LTN1_E3_ligase_6th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054478">
        <property type="entry name" value="LTN1_UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039804">
        <property type="entry name" value="RING-CH-C4HC3_LTN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12389:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE LISTERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12389">
        <property type="entry name" value="ZINC FINGER PROTEIN 294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22999">
        <property type="entry name" value="LTN1_E3_ligase_6th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23009">
        <property type="entry name" value="UBC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01197">
        <property type="entry name" value="FANCL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 16" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 15 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="551"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW34597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="607" mass="70695" checksum="8639702F6DE405F1" modified="2011-11-16" version="1" fragment="single">LSNFESQEINYTKLVKSSLKFVSVISGMSSFLTSKKLDRIRQYVFSEILGVRKESDIVEQGLKWLTYSILFYNIEDKADFLPIMKFTMVLNQISSWFDSSIAYDPEFIDIRVQVARFLGFVLQKQITIPDNYWDLTNQVLKDNLGVIQADPERADLKYYTFKLYNLINKISKDSVEDDYNDLLEIFLAEDSIQIDNQATVLNQQISQRALEESDIPTKVLINEKMKLVSTFSSSRSISTQRVTASYLRQVLLKEQEDFVVEYQLSKSKLGEDEEGGIEAKILDEFISIIRNMKYVVLELDDYDFTKYLWAWYLIFTYFEDSTFKIRSDYINQLSKHNELQVLFEFIAEHMDFSDKFFSSLVFKQDDGVIENRIPNYDLIETARTEDLKNEIKYLIVHIYYKCLNYCGSQVQYWFKQLRDKQLKGKIEKSSAKYVSSILITNIMEQVLQEKDKIQGKEENLSIKVNQVTNEIRTTYVIDEQKMEMVIKIPHNYPLANVTVEGPLRLGVKENQWKAWLLASQRIISLTNGSIIDSIELFCKNVNLHFSGFEDCAICYSILHQDLSLPSKTCPTCSNKFHAACLYKWFKSSGSSTCPLCRSAFNFRVGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJZ9</accession>
    <name>G3AJZ9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_148416</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373634.1">
        <property type="nucleotide sequence ID" value="XM_007373572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJZ9"/>
    <dbReference type="GeneID" id="18870783"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_148416"/>
    <dbReference type="eggNOG" id="KOG3017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035730_10_0_1"/>
    <dbReference type="InParanoid" id="G3AJZ9"/>
    <dbReference type="OrthoDB" id="337038at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442401" description="SCP domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="2">
        <location>
            <begin position="80"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="223" mass="24317" checksum="F5DC515406C4D55F" modified="2011-11-16" version="1">MKFSNVLSLALLSIAAQGAVLPGTDEADAPEVDVIAVAQRDISESDVPKIDVVAVAQRDVSQEQEVDIEKRGFGIVADILFVTEMFTLHNLKRLRHGVPLLNWDSGCYKHAQNLADSYDCSGNFPDPGNEYGNNQGTGNSNPVTVVADSAVVVDSWYAPSTSYNYGNPTAMNSFTNLVWKSTTKVGCAYKDCRSKKRGYYYTCSYDPAGNVPNFGKQNIFPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK19</accession>
    <name>G3AK19_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_59497</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373654.1">
        <property type="nucleotide sequence ID" value="XM_007373592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK19"/>
    <dbReference type="GeneID" id="18872943"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59497"/>
    <dbReference type="HOGENOM" id="CLU_3002270_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK19"/>
    <dbReference type="OrthoDB" id="4026095at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="57" mass="6681" checksum="BAFF6807F9C698CC" modified="2011-11-16" version="1" fragment="single">MNQYDEEDQRLPANLYVSNEELIDYLRDKIILNSKTISAVESKINMLLKLCSIKCFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK23</accession>
    <name>G3AK23_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_150117</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374349.1">
        <property type="nucleotide sequence ID" value="XM_007374287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK23"/>
    <dbReference type="FunCoup" id="G3AK23">
        <property type="interactions" value="242"/>
    </dbReference>
    <dbReference type="GeneID" id="18870855"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_150117"/>
    <dbReference type="eggNOG" id="KOG0610">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_84_1_1"/>
    <dbReference type="InParanoid" id="G3AK23"/>
    <dbReference type="OMA" id="HQKPPMI"/>
    <dbReference type="OrthoDB" id="432483at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05574">
        <property type="entry name" value="STKc_phototropin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000078">
        <property type="entry name" value="Serine/threonine-protein kinase nrc-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45637">
        <property type="entry name" value="FLIPPASE KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="470"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="121"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="277"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="787"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="870" mass="97358" checksum="0FEA56D3A931D1A3" modified="2011-11-16" version="1">MVSLSLFGNNSSPSSSNDVTPPSEQDLSSMEANVFHNGHAGITTPPHPGYSRPQSRNRKRSISFTKFLFNKDEPNSTNNHHVTPANDLGSSQSTLSVNHPEELKSQASFVRLKNMFKLSTASSLAHSESEQSEEQQEQQQQQHHQEPTSPDTSVHIGRNRSISTPLSGPIKNHVRNFASREAGYTPSQDSMSSSTSKIPTITTTPTSPPRSGKDESQVIHSNLYFAYQGLPPHMAHENPPHPAQYLHDSTNRSDISVALRHNDSVFSLSESGILKPPKVHTSQISTSDNDEEEQAIEEFPSTIKSSPAVPILEKPPKITELEQQKKHKTHPDQPQPQHVLRTPKKFVTATADDGIFDDDTDNLNLKPIDVERDLSVPVSPLQRALRRVASAPLVHRLLQEKSSDTTTIEPPFSTAATPKPVVKTEEEVYDINKHIGEVKITGRPRTYTADRTYSNAATRVTDVKVNADCFEKIRLLGKGDVGKVYLVREKSSNRLYAMKILSKKEMIERNKIKRALAEQEILATSNHPFIVTLYHSFQSQDYLYLCMEYCMGGEFFRALQTRDTKSISEQDAKFYAAEVTAALEYLHLMGFIYRDLKPENILLHQSGHIMLSDFDLSKQSERAKNPEVVFNRTGLHLSSTSSVSSRDGPALDTKACIDGFRTNSFVGTEEYIAPEVIRGKGHTSAVDWWTLGIFIYEMLYGTTPFKGSDRKKTFANVLKKEVKFLDSQPVSSQCRSLIKKLLIKDEEKRLGSKTGASEIKNHAFFRNTQWALLRNQKPPMIPVLTKSTKKVEKPIDEQTQPQDSAAVSLEENGKSDDPFAQFSSVTLQYEENECSSPMYNPESSAYTSVAYTMTKSQNGSPTKQKGFLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK51</accession>
    <name>G3AK51_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_60202</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374377.1">
        <property type="nucleotide sequence ID" value="XM_007374315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK51"/>
    <dbReference type="STRING" id="619300.G3AK51"/>
    <dbReference type="GeneID" id="18873272"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60202"/>
    <dbReference type="eggNOG" id="KOG1502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_9_2_1"/>
    <dbReference type="InParanoid" id="G3AK51"/>
    <dbReference type="OMA" id="FFLWIDV"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05227">
        <property type="entry name" value="AR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="334" mass="36934" checksum="2AAD1D336A2B2217" modified="2011-11-16" version="1">MSTTSVFVSGANGFIAQHIVKQLLSKGYNVVGSVRSEAKGEELKSLVNNDKFSYEIVPSLTEDGAFDDALKKHPEVTVFLHTASPFSFSVKDVENDLMKPAVSGTLNALKAIKEHGPQIKRVVVTSSAVAVFGFGPQFDANKVYNEDDWNPVTYEQALSNPMFGYFGSKKYAEKAAHDFVAKETVNFGISFINPPYVFGPQAYAIKDKTTLNTSNEVINKVVKLTKNDVLPAEGHVFVDVRDVARAHLVAFEKDEAVSKRLLLVSGSYTLDTIANIINKKFPQTTVPKGDESRNPETSKRIHKFDNSRTQKILGFEFKPLEESIIDTVDQIYNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK90</accession>
    <name>G3AK90_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peroxisome assembly protein 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Peroxin-12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_151827</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the PEX2-PEX10-PEX12 retrotranslocation channel, composed of PEX2, PEX10 and PEX12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375064.1">
        <property type="nucleotide sequence ID" value="XM_007375002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK90"/>
    <dbReference type="FunCoup" id="G3AK90">
        <property type="interactions" value="557"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK90"/>
    <dbReference type="GeneID" id="18870908"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_151827"/>
    <dbReference type="eggNOG" id="KOG0826">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031067_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK90"/>
    <dbReference type="OMA" id="QHYLARC"/>
    <dbReference type="OrthoDB" id="107372at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016562">
        <property type="term" value="P:protein import into peroxisome matrix, receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017375">
        <property type="entry name" value="PEX12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888:SF0">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12 PEROXIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Pex N-terminal" evidence="17">
        <location>
            <begin position="26"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="392"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="398"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW33549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="454" mass="52948" checksum="12680253895F66E1" modified="2011-11-16" version="1">MEYYSSLDASQLDSERPTLFEIISSNQLEALLSPSLRYILVHYASKYPRYLLKINNRFDEINLFFRGFIEWYFLKYWQGSFTENFYGLKRVNQTPLSQGDYNSNKLTSVVPSMVEERRQLTTLQILVSIFEVTGVAYLSEKMNYSYEVWYTKYVTNQLNTHESNTREENFKIQLKRKFVQIYPYLQSAYRLGNFVTILLYLSGSTKSPTLLTYLFRMNYSRLNQYDYSKNDSSVAELNPKTPLNEIAPPTGFEWLLRFANTNIWGPALNLIKFILGTFFPGAIFTLKFLEWWNNSDFSSKLAKNQGNVLDFTLPPPSTLTAALSASRKVVQEKKKKGRKPYVSGKECPLCKKELSNPAIIETGYVFDYSCIYNYLEKSHIIVSKKAESKKKELNEKDYSDDDEEEQQDEEAEEVTKIDINKGGRCPITGKKLLGCRWNAIREEWDIEGIRRLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKD3</accession>
    <name>G3AKD3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CCT-beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_60233</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin. In yeast may play a role in mitotic spindle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374405.1">
        <property type="nucleotide sequence ID" value="XM_007374343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKD3"/>
    <dbReference type="FunCoup" id="G3AKD3">
        <property type="interactions" value="1453"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKD3"/>
    <dbReference type="GeneID" id="18873289"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60233"/>
    <dbReference type="eggNOG" id="KOG0363">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_6_2_1"/>
    <dbReference type="InParanoid" id="G3AKD3"/>
    <dbReference type="OMA" id="CAEMVMS"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03336">
        <property type="entry name" value="TCP1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000002">
        <property type="entry name" value="T-complex protein 1 subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012716">
        <property type="entry name" value="Chap_CCT_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02341">
        <property type="entry name" value="chap_CCT_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="526" mass="57192" checksum="AB9C8A66D848FB6A" modified="2011-11-16" version="1">MSVQIFNEQATEERAENARMSAFVGAIAVGDLVKSTLGPKGMDKLLQSSTNPDAALVTNDGATILKSIPFDNPAAKVLVNISKVQDDEVGDGTTSVTVLSAELLREAEKLIEKKIHPQTIIEGYRIARTVSIEALEKHAVNNGDNPALFRNDLLNIARTTLSSKILSQDKELFSNLAVDAILRLKGSTNLNNIQIIKKVGGKLSDSYLDEGFILEKKFGISQPKRIENAKILIANTSMDTDKVKIFGAKFKVDSTSKLAELEKAEKVKMKNKVEKIKKFGINVFINRQLIYDYPEQLFTDAKINSIEHADFDGIERLALVTGGEVVSTFDKPENAKLGYCDVIEEILIGEDSFLKFSGVAAGEACTVVLRGATDQVLDEAERSLHDALSVLSQTTRETKTVLGGGCAEMLMAKAVDQAAANETGKRALAVEAFSRALRQLPTILADNAGYDSSELVTKLRSSIYNGIYTSGLDLNNGTIGDMRELGIVESFKLKRAVVSSAAEAAEVLLRVDNIIRAKPRTADRNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKL2</accession>
    <name>G3AKL2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Subunit I/j of mitochondrial ATP synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60954</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375132.1">
        <property type="nucleotide sequence ID" value="XM_007375070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKL2"/>
    <dbReference type="FunCoup" id="G3AKL2">
        <property type="interactions" value="83"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKL2"/>
    <dbReference type="TCDB" id="8.A.73.2.3">
        <property type="family name" value="the mitochondrial atp synthase stress-responsive protein (masp) family"/>
    </dbReference>
    <dbReference type="GeneID" id="18873654"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60954"/>
    <dbReference type="eggNOG" id="ENOG502SC94">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174950_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKL2"/>
    <dbReference type="OMA" id="KPMWPFY"/>
    <dbReference type="OrthoDB" id="5520611at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006995">
        <property type="entry name" value="ATP_synth_F0_jsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28060">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT J, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28060:SF1">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT J, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04911">
        <property type="entry name" value="ATP-synt_J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="63" mass="7147" checksum="D600E56A08A3E528" modified="2011-11-16" version="1">MALPRYGFPLVKTYWPYFAGAGITYFLIYKASQASMNSAEFINDPRHPRFASGAKVIDLENKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKM6</accession>
    <name>G3AKM6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_136071</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375146.1">
        <property type="nucleotide sequence ID" value="XM_007375084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKM6"/>
    <dbReference type="STRING" id="619300.G3AKM6"/>
    <dbReference type="GeneID" id="18869939"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136071"/>
    <dbReference type="eggNOG" id="KOG1827">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035525_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKM6"/>
    <dbReference type="OMA" id="FQCKLFV"/>
    <dbReference type="OrthoDB" id="1742084at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04369">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037382">
        <property type="entry name" value="Rsc/polybromo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062:SF19">
        <property type="entry name" value="PROTEIN POLYBROMO-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062">
        <property type="entry name" value="SWI/SNF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="48"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="192"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="308"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="330"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="594" mass="68011" checksum="B6E37AA839F7C5AC" modified="2011-11-16" version="1">MAPKRKAASASAGSAKKSKQPSSHSTEDLKQFFQSTLTLVMNLREEENDEQMAFPFVKLPSKKLYPDYYQIIDQPISLSDIQKRIKTYTTCDEFLADFQLLLDNATKYNNADSWIVINANKIVEFVKDQVKEFENSSVKTEEEHHPKIKLKLKKKEVKEEPQQQEEVGITNADLPDLCLGILNDVIDHEFDDEGVISAPFIEDVDTDYYSDYLDFVTKPMSFNKVIQLIEKKKLFNSKASVSDNLQKFHETTALIFNNARLYNDESAQIHQDANMLEEYFDEKFNELKTKAEGSSKLKLKISKPKLKLSVHKEETPKKPRKKKEVKPKEEDEDEVVEEEDVADEVKTEPVEKKEAKQIIVEPSIHNTLGKTLPLLPRESSIIQETSIFSSVPVSTQIAQYVQQKTIPNNLLPLPRNQDIKKSLFPTQPLHSMATIFEYKVPANGYTSQSYTVALPNDISPFVSFKASLHNLLYEVKREDLVNGHGYLNSTSDEEFQCKLFVNDEEVTNANDCFEEKRTDDDVLGVQYDVKLSYGLNVLSFECKVAPHLSKQIKPTAVSEPNEEIAGRHTRHQLQQMKMSWDVEKITFYVVCNNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKU5</accession>
    <name>G3AKU5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_60982</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375161.1">
        <property type="nucleotide sequence ID" value="XM_007375099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKU5"/>
    <dbReference type="FunCoup" id="G3AKU5">
        <property type="interactions" value="292"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKU5"/>
    <dbReference type="GeneID" id="18873667"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60982"/>
    <dbReference type="eggNOG" id="KOG3422">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078858_0_1_1"/>
    <dbReference type="InParanoid" id="G3AKU5"/>
    <dbReference type="OMA" id="MPGMYEF"/>
    <dbReference type="OrthoDB" id="268521at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.10:FF:000010">
        <property type="entry name" value="60S ribosomal protein L16, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="entry name" value="Ribosomal protein L10e/L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047873">
        <property type="entry name" value="Ribosomal_uL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000114">
        <property type="entry name" value="Ribosomal_uL16_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020798">
        <property type="entry name" value="Ribosomal_uL16_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_uL16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_uL16_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01164">
        <property type="entry name" value="rplP_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220:SF13">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL16M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00060">
        <property type="entry name" value="RIBOSOMALL16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="Ribosomal protein L16p/L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00701">
        <property type="entry name" value="RIBOSOMAL_L16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW33646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="227" mass="25273" checksum="2A1D7BB01EAD5548" modified="2011-11-16" version="1">MLGLARNLFSSFRTIHAPTPSSLLNTQNFGVFKRYTHEYAPRLNRIGKAMKGRVPVRTGGSTKGNSVESGKLGLRLKSQGLRISANQLKAADKVLRRELRPTKSLLITRFACNIAVCVKGNQTRMGKGKGGFHHWAARFPTGKVLFEIKGDIHDRTAREALRKAADKLPGLYEIITPESKVRVGLKSLVDKPEPVNYVAQMNATPSKKWANIQAHKTNPVYKLFRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL00</accession>
    <name>G3AL00_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome b-c1 complex subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Complex III subunit 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_60362</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The complex plays an important role in the uptake of multiple carbon sources present in different host niches.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein, 2 core protein subunits, and additional low-molecular weight protein subunits. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with cytochrome c oxidase (complex IV, CIV).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion inner membrane</location>
            <topology evidence="1 11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UQCRQ/QCR8 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374558.1">
        <property type="nucleotide sequence ID" value="XM_007374496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL00"/>
    <dbReference type="FunCoup" id="G3AL00">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL00"/>
    <dbReference type="GeneID" id="18873365"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60362"/>
    <dbReference type="eggNOG" id="KOG4116">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156007_0_1_1"/>
    <dbReference type="InParanoid" id="G3AL00"/>
    <dbReference type="OMA" id="QWAIERN"/>
    <dbReference type="OrthoDB" id="6683853at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.210:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.210">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004205">
        <property type="entry name" value="Cyt_bc1_su8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036642">
        <property type="entry name" value="Cyt_bc1_su8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12119:SF2">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12119">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX UBIQUINONE-BINDING PROTEIN QP-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02939">
        <property type="entry name" value="UcrQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81508">
        <property type="entry name" value="Ubiquinone-binding protein QP-C of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW33043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="95" mass="11082" checksum="D1E962EA6BEA8CF1" modified="2011-11-16" version="1">MGGLPEPRTYLGWWGHLGSPKQKYITQYTISPYAAKPFKGAAYNAVFNTFRRTKNQFLYVAIPAVIVWNIWTNARDYNEYLYTKAGREELERVNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALA4</accession>
    <name>G3ALA4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60452</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374662.1">
        <property type="nucleotide sequence ID" value="XM_007374600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALA4"/>
    <dbReference type="GeneID" id="18873420"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60452"/>
    <dbReference type="HOGENOM" id="CLU_2098357_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALA4"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="116" mass="12786" checksum="64C2461603368939" modified="2011-11-16" version="1">MPSVEVEVLSVQEHFQEEQFSQLSVWELVVEVASEQVVEVEQVVEVEQVVEVEQEQVAEQASVLEEDPTPVLIQPNNDFGDPVPVAVADSGNVLLPSMSNLVLLLVTVLHLIALLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALB9</accession>
    <name>G3ALB9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_60472</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374677.1">
        <property type="nucleotide sequence ID" value="XM_007374615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALB9"/>
    <dbReference type="FunCoup" id="G3ALB9">
        <property type="interactions" value="1032"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALB9"/>
    <dbReference type="GeneID" id="18873429"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60472"/>
    <dbReference type="eggNOG" id="KOG0658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_20_1"/>
    <dbReference type="InParanoid" id="G3ALB9"/>
    <dbReference type="OMA" id="MKTTMPM"/>
    <dbReference type="OrthoDB" id="272141at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000009">
        <property type="entry name" value="Glycogen synthase kinase-3 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF0">
        <property type="entry name" value="PROTEIN KINASE SHAGGY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="24"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="283" mass="32657" checksum="42FF8472689EDC09" modified="2011-11-16" version="1" fragment="single">MSKIEIVTENVTNGHSGAIERIQYTQSQMVGHGSFGVVFQTQILPTNEIAAMKRVLQDKRFKNRELQIMKLVHHRNIADLKYYFYTNNDKNELYLNLILEFVPETLYKASHYYVSKRLSMPPLEVKLYTYQMFRALNYIHSQGICHRDIKPQNLLINPDTGELKLCDFGSAKILNPDEPNVSYICSRYYRAPELIFGATNYTTKIDVWSAGCVMAELILGQPLFPGESGIDQLVEIIKILGTPTKEQIRNMNPNYMEHKFPQIKPIPLTKIFKKIGNDCILFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALJ0</accession>
    <name>G3ALJ0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Actin cytoskeleton-regulatory complex protein SLA1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Actin cytoskeleton-regulatory complex protein sla1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_50135</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Actin patch</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SLA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374748.1">
        <property type="nucleotide sequence ID" value="XM_007374686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALJ0"/>
    <dbReference type="FunCoup" id="G3ALJ0">
        <property type="interactions" value="159"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALJ0"/>
    <dbReference type="GeneID" id="18871408"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50135"/>
    <dbReference type="eggNOG" id="ENOG502QQC3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003674_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALJ0"/>
    <dbReference type="OMA" id="FMAQGED"/>
    <dbReference type="OrthoDB" id="26539at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990964">
        <property type="term" value="C:actin cytoskeleton-regulatory complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140224">
        <property type="term" value="C:SLAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140312">
        <property type="term" value="F:cargo adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000147">
        <property type="term" value="P:actin cortical patch assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034316">
        <property type="term" value="P:negative regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09532">
        <property type="entry name" value="SAM_SLA1_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11773">
        <property type="entry name" value="SH3_Sla1p_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11774">
        <property type="entry name" value="SH3_Sla1p_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11775">
        <property type="entry name" value="SH3_Sla1p_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000094">
        <property type="entry name" value="Actin cytoskeleton-regulatory complex protein SLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000300">
        <property type="entry name" value="Actin cytoskeleton-regulatory complex protein SLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.700">
        <property type="entry name" value="SLA1 homology domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056996">
        <property type="entry name" value="PH_SLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007131">
        <property type="entry name" value="SHD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035800">
        <property type="entry name" value="Sla1_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035821">
        <property type="entry name" value="Sla1_SH3_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF19">
        <property type="entry name" value="ACTIN CYTOSKELETON-REGULATORY COMPLEX PROTEIN SLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24081">
        <property type="entry name" value="PH_SLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03983">
        <property type="entry name" value="SHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 16" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="18">
        <location>
            <begin position="4"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="18">
        <location>
            <begin position="75"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="18">
        <location>
            <begin position="372"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="135"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="440"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="710"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="774"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1036"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1174"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="161"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="226"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="440"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="787"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1179"/>
            <end position="1195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007948"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020357"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW33233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1202" mass="131942" checksum="9E8F686B397F865E" modified="2011-11-16" version="1">MSNIYIGVYKALYDYAAQAEEELTIQTDDILYLLEKSDVDDWWKVKKRVLAQGDEEVEEPFGIVPSNYIEEAPVIQQASALYDYDKQTEEELSFKEGEKFNVYDLNDPDWILVSNSTKTEFGFVPSNYIELGTATASQPQQPQTVQPEPAQQAPVQQQIPQPAPMIPVSNFPPPPQRSVEATPETAPTPPVKDFPRETEPLRSSYYDREDEEDDAPPPMPTRPRESSSAAATSAPSNNYEQVSDVGKKDHEFDGEYFTWYIDEIDGRKKLAVVFSIGHGFIIFKPNKQGSKLLRQASSLENQWRIRDLISFSNEKKHVFLEFKHPTASIELHAGSKDVAEAIMSILGDLKGAESAKGLKEVARASKAKTGAGNRKIARLLYDFDSQGDDELSGREGDEVYIINETKSKDWWMVENIETGRQGVVPSSYLEIISTSNLDKLTDRPARTRSTKKEKSSQHHHRSREERDKIREKDKQQREKQVTKHEEQQDKSMPNFHRVRTWIDSTGTFKVEAEFLGCYEGKIHLHKTNGVKIAVSCVKLSIEDLEYVEKITGTSLEHYKEEILKHTQAKAKPKAPEEMKKTPSATAVINDVSKPQPSRAKATTKLTVGEPDYDWFEFFLQCGVDIGNCQRYSLSFSREQMDENILEDISPSLLRTLGLREGDIIRVMKFLDNKFDRKKTTSEEPAPLFVNTNGELKNNTSVQADSKVNAAALPSPVKATPPTVTEPPKQPQKIEDDAWAVKPAARSTEDLLKPVQPEAAQYTGSLSDLVNIKPVETKNEPPAPQPQQPAQAPSAPALQPVKTSNTLIQPGQQFAVQQTGVSNMIPVPAQRTGTLVPVQRTGGILAAQPTGFVPITAQPTGFIPIQATGIIQPQLTFGIIPLQTGSTTFNTGITRSGSAPPATSFGQPVIMPGNVTMPQTTFGTQITGGLPQTTFGTQVTGGLPQTTFGTQVTGGLPPTTFNQQPIVPAQRTGGQISGGFVPQSAFGKQITGGFMGTNVVPQTSFGQQPAPAGFPQTSFGTQITGGLPPTSFGNQITGGLPATSFGQAPVNPFPQPPQQQQQQQQPTFGGVSQGFNQFAQQATANPFPQQQQPMGQFGQPQQTFNQFPSQQPTQQFNMFGQPQQQDMSQMTNMFQNTSISAPASINAGQMPTTSFGQAPQFEGFGTLQSQPTGMGFGNAPLQSQQTGRRANIQAATPDNPFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALQ0</accession>
    <name>G3ALQ0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U3 small nucleolar RNA-associated protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_137181</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
            <location evidence="1 5">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the NRAP family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374808.1">
        <property type="nucleotide sequence ID" value="XM_007374746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALQ0"/>
    <dbReference type="FunCoup" id="G3ALQ0">
        <property type="interactions" value="1047"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALQ0"/>
    <dbReference type="GeneID" id="18870024"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137181"/>
    <dbReference type="eggNOG" id="KOG2054">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003502_1_0_1"/>
    <dbReference type="InParanoid" id="G3ALQ0"/>
    <dbReference type="OMA" id="DERAHIP"/>
    <dbReference type="OrthoDB" id="10251401at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005554">
        <property type="entry name" value="NOL6/Upt22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035082">
        <property type="entry name" value="Nrap_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035367">
        <property type="entry name" value="Nrap_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035368">
        <property type="entry name" value="Nrap_D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035369">
        <property type="entry name" value="Nrap_D4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035370">
        <property type="entry name" value="Nrap_D5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035371">
        <property type="entry name" value="Nrap_D6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17972:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17972">
        <property type="entry name" value="NUCLEOLAR RNA-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03813">
        <property type="entry name" value="Nrap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17403">
        <property type="entry name" value="Nrap_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17404">
        <property type="entry name" value="Nrap_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17405">
        <property type="entry name" value="Nrap_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17406">
        <property type="entry name" value="Nrap_D5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17407">
        <property type="entry name" value="Nrap_D6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Nrap protein" evidence="7">
        <location>
            <begin position="188"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="8">
        <location>
            <begin position="340"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="9">
        <location>
            <begin position="492"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="10">
        <location>
            <begin position="664"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="11">
        <location>
            <begin position="853"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="12">
        <location>
            <begin position="1020"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW33293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1152" mass="131162" checksum="F52FEDECE1A371BE" modified="2011-11-16" version="1">MAKRKLEDTNEASSDLAKDETEFTSFSDREEEKLNGEGHEDEQENSDEEESDEEEEEEEESRPTKKPKKQLSAQDVQVARETAELFKSNIFKLQIDELLKEIKIKDTHVVKLEKVLHRLYDLISQVPEVNDLSLQQVEAMFNSKKIVIPFPDPKPTKLNYKFSYLRPEDVALVGSFGLKTCTTSSRSIDVALTMPKSLFQPKDYLNYRALYKRAFYLTYLSQQLIPLSKKNNLPIKISYHYLNGDVLNPVLSLQSIETDNPEDLHFNKTKFTINLIAALPFGNFECKKLLPDKNCIRVQTENEDTALPPTPIYNSSILSQTSYDYYLKYLYTIKKTTDGFKDACILGRLWLQQRGFTSDINKGGFGHFEFAMLMSILLNGGGVEGNKILLHGFSSYQLFKGVVKYLATMDLTNGYLSFSSLIGEKTTSVYKSEGFQVPTVFDKNIKLNLLWKMTRSSYQELQTKAKETLLLLNDVVTDRFDSILLQNSNFNQLKYDLVLNLTIPDKLIESFNPLEKISFITFENYIKNKLSIILKTALGDRISLVHIESEETTTTFPINKRKPNTNNSSYIIGLTLNAEESDKLVTKGPNNEDKEAGLKFRSFWGSKAQLRRFKDGTIQHCVVWTATNEPIVLTIMKYVLDVHLYQDISMNLTSEITHFNNKLPVPLSGTSTSLANFTALRNSFEQLNKVMVGLDLPLNIKSVLPASAGVRYTSLLQPVPFAVSNPDFWNECILQFESSTRWPDELSTLEKTKTAFLLKINEVLKKTNYTSFITKDDSIPYNENISLLNVLTPEGYGFKFRVLTERDEVLYLRAVDNSGKNKALLQDVYLKFNAKYLGVVKHTRTISVLAQHFHGYSATVRLFKQWLDSQLLLSHFSNELIELIAIKPFVDPAPYSIPHSVESGFLQILNFLATWNWKQDPLILDLVKSTADDKLSDKLTIQAYRVIQSNFDKIRKSDPLGLKTQLFIGSKDDPSGILWSNEITLPIATRLTALSRAAINLIKTQGITESNLDLIFTPALHDYDFVIHLKSSDLTVSSGILPPNTFKNLIQPLTSFPEDITTKYDLTQAYFSELNDKFGNSIIFSIRKFPCNDNVICGLFMPDHLSKKKFRVNLGYNVKPTEDESVIINKDAIYDQIKIMGGDLIKSIDIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMB0</accession>
    <name>G3AMB0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Monocarboxylate permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_60767</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374923.1">
        <property type="nucleotide sequence ID" value="XM_007374861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMB0"/>
    <dbReference type="STRING" id="619300.G3AMB0"/>
    <dbReference type="GeneID" id="18873556"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60767"/>
    <dbReference type="eggNOG" id="KOG2504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_1_0_1"/>
    <dbReference type="InParanoid" id="G3AMB0"/>
    <dbReference type="OMA" id="QISWIFG"/>
    <dbReference type="OrthoDB" id="6509908at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032218">
        <property type="term" value="P:riboflavin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF177">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER MCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="301"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="391"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="426"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="461"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="81"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW33408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="490" mass="53813" checksum="1A611E8FE1DB5F39" modified="2011-11-16" version="1">MSGSSITTISIPERVHVIELTNNLKDNDISVCNYEANPPNQDTSMRRRRGNTVSSDESRALMYGSEVDPNAFPDGGLEANLVLVGSFIGLIADFGVANALGAIESYVSYHQLSNISKTDVGWVFSLHLGVMYFGGVFFGELFDRFGAKRPLIAGTFFMCLGLFCTAESHTLYQFMLSFSIVTAIGTSIAMSPLIGALSHWFLKKRAMACSIATIGGLVGASCFAIMLQKLYESVGFRWAIRIQAFICLACMSVAIVLVRERRQNVPDQEIPSDVEKQNLFETGKNFFKQALDFSMVKDSRFILLTFGVALAEIVSMTTLTYLSSYALAYHISDTRSYLLLTIVNVCGIPSRLLSGILADKYGRFNVMVFTSMLTTITIFAIWFPAKGNISILYAFGILFGISTSAVISLIPACTGQICSSDKFGKVYGTLYFFLGFLTILGMFFASLVIGKGDTSSYRNFVLFEGGLSAASIFFWIAARYSNVGWQLCKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMG5</accession>
    <name>G3AMG5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinase</fullName>
            <ecNumber evidence="4">2.7.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_137738</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <dbReference type="EC" id="2.7.-.-" evidence="4"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374332.1">
        <property type="nucleotide sequence ID" value="XM_007374270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMG5"/>
    <dbReference type="FunCoup" id="G3AMG5">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMG5"/>
    <dbReference type="GeneID" id="18870070"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137738"/>
    <dbReference type="eggNOG" id="KOG1620">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042569_3_0_1"/>
    <dbReference type="InParanoid" id="G3AMG5"/>
    <dbReference type="OMA" id="FRICGMK"/>
    <dbReference type="OrthoDB" id="338650at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000827">
        <property type="term" value="F:inositol-1,3,4,5,6-pentakisphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000825">
        <property type="term" value="F:inositol-1,3,4,5-tetrakisphosphate 6-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000824">
        <property type="term" value="F:inositol-1,4,5,6-tetrakisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008440">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000823">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 6-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000821">
        <property type="term" value="P:regulation of arginine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF103">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE MULTIKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="347" mass="39290" checksum="4A0466FC82574C64" modified="2011-11-16" version="1">MATLSPYVPSKHQAAGHDGCLTLEDGSLFIKLTNSQEIDFYAKTQADDSDGNERIPGTKLVDWMPLFMGTLSETDVNSVAAPGHIHGPSQPTDKQYVVLGNCYSGYAHPSILDIKLGAKLTDDEVTAPEKIERLQKVSESTTSGSHNFRICGMKVYNGESVDKPNELYEKMNDSSVSINSSGEKDNYLEFNKFYGRSLDSKNIKDGIRLFFTHHLKKVPNGQEIVIRLLDRFHKRLQLLYNCLLDYEVRIFSGSLLFIYENDLQKWQTALADEEEYYQTYDPLIRECNLADDDEEEEEEEAPVSSLNIIDFAHAKYVPGQGHDENILQGIENLIDIFDQLLKEEESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMS7</accession>
    <name>G3AMS7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_50395</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375036.1">
        <property type="nucleotide sequence ID" value="XM_007374974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMS7"/>
    <dbReference type="GeneID" id="18871436"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50395"/>
    <dbReference type="HOGENOM" id="CLU_551157_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMS7"/>
    <dbReference type="OrthoDB" id="10538103at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="47"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="231"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="309"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="366"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="176"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="373"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="495" mass="55001" checksum="FA2C176996523F51" modified="2011-11-16" version="1">MANLDESTEEINRRFGIPNLNNRKSTFPDLTLASLSDEDIDFDKDLGLNSSGILDSNTRNNSPKKSNMRNYGSPPKRVSNDYQDHMYRMRSNPSSPIKHKKTPNRRNLFYEDLNEPTENFEFAGTHAEVGDIGAKKIKAAIDGLSSRKPDQDVNYRDLYNDAHKLSTQLVVKSAGLEKENARLRDIERKNNQTIAELQNWVTSLQEKTKKYKQLYLKAKKKLDEVDQERTEEVVEKVNIPSQPQQERPSSRTKESVGVPLDEVKSLLENLNGSIEKLINKDNTEPKPTVDEKIIHDICEELVRKVKHGLDATPTDPEPPVQYFSTQPRPAFTSTQVPNPPPTNPPSTTYVPYAPIPEPFVAPAATTPSTAAAAPPPPTTNVPVPAPAPSSSPSPAPVSTHAPVQSTQPDAEAPKSTPEEPPITHAQLKELINALTNQKLADPVDEKDPHIYVDCRLCFNAKDKSNIKDGICKRCTKEVMSCDTTLNKLGGGWLPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMV4</accession>
    <name>G3AMV4_SPAPN</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">SPAPADRAFT_61448</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Saccharomycotina</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375644.1">
        <property type="nucleotide sequence ID" value="XM_007375582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMV4"/>
    <dbReference type="FunCoup" id="G3AMV4">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMV4"/>
    <dbReference type="GeneID" id="18873899"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61448"/>
    <dbReference type="eggNOG" id="ENOG502S1HU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077719_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMV4"/>
    <dbReference type="OMA" id="PWKCIDL"/>
    <dbReference type="OrthoDB" id="3994490at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018555">
        <property type="entry name" value="C630.06c-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09428">
        <property type="entry name" value="DUF2011"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="205" mass="23663" checksum="F8156780F26B448F" modified="2011-11-16" version="1" fragment="single">MKEQKISRSHLYDDSQSEYESETESLGYSAPNLDFEFIEVDTSEPQEVEENESKVEKQEEEEFEFPLFSMGGGGGGGSSTDGKAADEPKQPMKVSLKEAEEEVINQERPESYYIAKYTPDQSRQFEVCALDAKSIIEPVYPSDDPRPWKCIDLNKYNAQIELVKNKEKRKSRPGKKKRETKIACRERRIERVQLAKKLQVEQKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMX5</accession>
    <name>G3AMX5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Alpha-1,2-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_71814</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375664.1">
        <property type="nucleotide sequence ID" value="XM_007375602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMX5"/>
    <dbReference type="STRING" id="619300.G3AMX5"/>
    <dbReference type="GeneID" id="18875396"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_71814"/>
    <dbReference type="eggNOG" id="ENOG502RGFY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013298_2_0_1"/>
    <dbReference type="InParanoid" id="G3AMX5"/>
    <dbReference type="OMA" id="YQYKMLA"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646:SF1">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442442" description="Alpha-1,2-mannosyltransferase" evidence="11">
        <location>
            <begin position="25"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="43"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="614" mass="71622" checksum="B1BA00841A978E6C" modified="2011-11-16" version="1">MMRPKYRWLLLILLLLIGLHLRSSQSGQSGNNLASILENSLNRGHTQRNKRPQPGEHLDKPQDEAIPKKSGGGWFSKDRFAQPRPLSRFKIKKIRDYTIFFDKLEKFAIKQPSIKDRYKTEKAHEMFSTHVTHNLNKEYLENVLDISDQTYKELKDSHHRYVTEQIPKLINDYGISTFGNILPSDKDWKKYHGSSGYVLVGGGQYTWLSYLVIKQLRATGAKFPIELFIPSFEDVDQEFCDKVLPKFNARCNVFDSILSRKLKERFDIGGYQYKMLAILSSRFENVLYLDSDNFPARDPDYLFESDLYRKNQLLLWPDAWARTTNPKFYEIAQVDVKPNKLRYTDYDVFMAGGVENLKPLTAYTFADSWFHDFENTLPDPTSETGMLLINKTSHLKTLLLCLYYNVFGPHYYYPLLTQGSAGEGDKETFIAAAHVMKEPWHQTLKQFKWTGYYSKNDGKFASKALAHYDPIDAIEDPTAGDVDVIFMHLSYPKFYPNWLVDNHDLIYEDGEHIRMYSQINENVGYDFDLRVLQFFTEGLCHNYYDASGKPIDGTAGVSLQENYMGRYLSYVAQDFEENTKRCEEVFIPHLMWLKKTEGQEYLHDFYKAADEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMY7</accession>
    <name>G3AMY7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_153273</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Required for vacuolar assembly and vacuolar traffic.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the VPS41 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375677.1">
        <property type="nucleotide sequence ID" value="XM_007375615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMY7"/>
    <dbReference type="FunCoup" id="G3AMY7">
        <property type="interactions" value="709"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMY7"/>
    <dbReference type="GeneID" id="18870947"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_153273"/>
    <dbReference type="eggNOG" id="KOG2066">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001285_2_1_1"/>
    <dbReference type="InParanoid" id="G3AMY7"/>
    <dbReference type="OMA" id="PQLVWQD"/>
    <dbReference type="OrthoDB" id="244107at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035542">
        <property type="term" value="P:regulation of SNARE complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006624">
        <property type="term" value="P:vacuolar protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099022">
        <property type="term" value="P:vesicle tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000933">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057780">
        <property type="entry name" value="Beta-prop_Vps41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016902">
        <property type="entry name" value="Vps41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 41 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23411">
        <property type="entry name" value="Beta-prop_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23556">
        <property type="entry name" value="TPR_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028921">
        <property type="entry name" value="VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00299">
        <property type="entry name" value="CLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="Vps41 beta-propeller" evidence="7">
        <location>
            <begin position="50"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="5">
        <location>
            <begin position="646"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="10"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR028921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="925" mass="106787" checksum="D045F2977A9AB223" modified="2011-11-16" version="1">MPQHNPDSIEETHTVPNEYEKNTAPELIHHDEYNSSEEDSDEEDDSPPKLKYTRLNKLPPNFFNKDPVSTCCIHDSYFIFATHSGVIHISTPDFQPIRTFKAHRASVLSIYTDGTYFATASMDGTVVIGSIVDEKDIVAYNFQRPVHAVVLDKHYSTNRSFISGGMSGKVILSNRSWMGKRTDVVLDEGSGPIVSLERVDDLLFWMNDKGINIYNFSLKQMLLVVDKPEDSPRSDLYWPRVTFPDPNRIIIAWSNYIWSLKISIKQNSDEKVTSSGMSKILPSTASISFRNIQEKEVEIEHIFKVDSLICGITSFTDDLWMILSYDPPTLDDTTGKLQFNNPDLKLINSVTGEVEFEEELGLKNVENLGLNDFTLGSHIETSTKYYIISAKDAVVAQELTLNDRLSWYLETEEFLHAWEISEHLVSPIKRLNYGIQYVDSLIKEDDWVSAGKYLQKFLDIKDIKDNETRSGTITSSSTQDDSYAKEIVNQWEIWSNIYINSGHIIDLTDIIPITSDLPKDIYNQILKYWINTNQEKLHDLITQWDIEVYDVKQIQSQLEDIVRTEDSSQLEKSLVLLYDKSLEPAKAIPHMVHLKDHNIVEYLSKNHILSQFINELPDIISLRFDKDELETLPISKIQSKISDIVSTLVDKRLEVSPKVIVELFSSMPFISFFYLEKLNEIDSYLVAPFGNERVKLYSQFKRESLLPFLHKNTGYDIDEAINICEQNEYIEELVYLLGRIGQNKKALELITTRLNDPIMAIKFAKRQNDKEAWNILLDYSMEKPDFIKALIENSDESSNAYYDPITILKRMPKDIKVPGLNDSVIEFSKNNDLNMILNQIILRIIYQQSKETSQAYKLRRLKGEEIEVDHMTNIIQTFETIVLLRNNENTDLQLEANLTSDPHNMSYETHMKKLLHLKELQRKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMZ2</accession>
    <name>G3AMZ2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61472</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375682.1">
        <property type="nucleotide sequence ID" value="XM_007375620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMZ2"/>
    <dbReference type="FunCoup" id="G3AMZ2">
        <property type="interactions" value="401"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMZ2"/>
    <dbReference type="GeneID" id="18873911"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61472"/>
    <dbReference type="eggNOG" id="KOG1455">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026209_5_2_1"/>
    <dbReference type="InParanoid" id="G3AMZ2"/>
    <dbReference type="OMA" id="RQFNDMF"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="38"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="300" mass="33952" checksum="876CED5130BF0925" modified="2011-11-16" version="1">MPAEIPYKPKGKPTIEFIDYNGAKFKTVFWSVPEGVANKGRLIYVHGFAEHADLYTQFFDDLSQQGYEVFYFDQRGAGETSPGSLVGKTDEFHTFDDLDYFIKYNLDGVTDKTKKFFLVGHSMGGGIVLNYGIRGKYVDNIRGIVASGPLVKLHPESEPNFVLRALAPVINKLLPSMKFDSALNFDNITTNESWKNYIMSHDKKLIGTIRQFNDMFARGDELLVPEKVKKFNPKVPVLVIHGTEDHINDIEGTAKFYGLLPDTVDKKFVRNEGAKHSNFIESEKLYKVALDETVAFFDSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN54</accession>
    <name>G3AN54_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_60990</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375173.1">
        <property type="nucleotide sequence ID" value="XM_007375111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN54"/>
    <dbReference type="STRING" id="619300.G3AN54"/>
    <dbReference type="GeneID" id="18873672"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60990"/>
    <dbReference type="eggNOG" id="ENOG502RYEB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033255_0_0_1"/>
    <dbReference type="InParanoid" id="G3AN54"/>
    <dbReference type="OMA" id="IGTHHYD"/>
    <dbReference type="OrthoDB" id="843225at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47815">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN A FAMILY PROTEIN C25B2.10"/>
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        </location>
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            <begin position="97"/>
            <end position="107"/>
        </location>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="382" mass="43625" checksum="255656717E03E459" modified="2011-11-16" version="1">MVTVKIEPPIEEEASEDGAPLEKKKSMVEVEDETDINTRLNAMLYQTSTDSVELYKLQNNSSDLLGRLYYDDYDSEITSPVSSNLGTPLLAPQPGTSISSDSSGNSSQIRPGISRGRSFERGISFDNTNLETKKQSLILKVKHPDFKFRRNNKTFLTGYNNDYESFKAIEWLFEEMVINGDTIVIFQVLNEKHHDRIDKQIANVNLQKFEKLNMDHHMKKVSIIYEIVIGKAEKNLKKAIDEYSPSMMIVGTHEGKEHKSFMKSSTSKHFLECALVPVIVVKPSYHYVEQLKSPIDSEQYFENWMKSIDAPVTREKPKIRGILSPTHSRNSSYTNLVAEERGRKINTLLVPTRSRSSSKTRDKEPRSRSSSRTRFAKFLLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="396" mass="46301" checksum="8B5A9AB54F296DB6" modified="2011-11-16" version="1">MALCANKADFAVNSYCLVQVMNQVKVENEEIAMILKFLTSDVHGYYFGNYMYNKITNEELQKISQTQLNEIATQIIDVNLNNGDCESAFAGWSKVASLVQPERCMHSLLNLLHSTETTELILEVLTNLPQEVLDTDPMVDFQLEFYGTRDEYISQFDSLIPKLTHPLRRSTLTSFLKVFLHRNDEPKTDKVIDNIFNHQTGIQPKELNWIIKKLLCHDKHTEALAMVRKINNVNVTALSYVSIFKYIANKYDSDHESKFQPAFEEICMKMLRSNDRSVHEKFTVEVFNHLAELDIRYAIQSYMKVRKSQKPIRFNHFGMPLQFNQILKFSQKNTAQILQTLSIEAVKHEDSESFQWAISEYRRNGWTIERIVKMLKQHDKHSFLERQFKPEVLNCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANV2</accession>
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        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EGW32577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="eggNOG" id="ENOG502SFHW">
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    </dbReference>
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    <dbReference type="InParanoid" id="G3ANV2"/>
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    </dbReference>
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    </feature>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
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    </evidence>
    <sequence length="480" mass="54759" checksum="5253586F3B6BFCA8" modified="2011-11-16" version="1">MYNYSSAYQGYNLNPPTSNAASNNGNLQNNNNNNGNSNNNSNAGTNPQQGQGVGVGVVPSATNPMLSDRGKNIRHNMLQNTAPYYSRTSPVPPLSHQMHHQPHTSHRSALGTGVPNMGLPSVQQIRHIQPQQTQHAQQQGQNQSQNQQYTQYQQQQQQQQTSPQPTTQQIFASQLTNDHQPQQLDPSQLSAFTPQQQQQYQQSIMQQQYLQQHQQQQHQQPQVVQPQQVQPRQYSTVQMQQIHQQGHMSLQQPQQQQQQQQAPTGSISPEQQQQQPPQKKPRKQRVTKKQLEQQKELEQEQRRQQQEQQELLKVQQQYQLTADGKKKRGRPKKLIHDPTTNTFIDSSHPHFKALNKTMKEAAAATAHVDGSNGNLVQTQNLQQQQQPQQQSPPQQTQPKKKQRRTNDAARSAKNENLSPSLSRLFNQPPSMRTLDDEAVQQLLQKKDKRGRPRKFPVEETGITIKGIRVNGNVKHRVQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANY8</accession>
    <name>G3ANY8_SPAPN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
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        <name evidence="1" type="ORF">SPAPADRAFT_139735</name>
    </gene>
    <organism evidence="2">
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        <lineage>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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    <dbReference type="Proteomes" id="UP000000709">
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    </dbReference>
    <dbReference type="GO" id="GO:0042720">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024645">
        <property type="entry name" value="Mitochondr_Som1"/>
    </dbReference>
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        <property type="entry name" value="Mitochondr_Som1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
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            <dbReference type="EMBL" id="EGW32613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="131" mass="15100" checksum="7B0ABF0182E150D6" modified="2011-11-16" version="1">MAPPIPVYSANEIKGQYSEQLNNPEKYECQLKSLTQHECTFRPASLDGSRPLEIICLPFKRIFQRCLIPKTIKKDGQKVVVKNWVNIEITNSETNQDLFDPNSKYAGDVKDFMSTEHELKKFLEQEAEGHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP10</accession>
    <name>G3AP10_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Aureobasidin A resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_61139</name>
    </gene>
    <organism evidence="9">
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        <lineage>
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    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375317.1">
        <property type="nucleotide sequence ID" value="XM_007375255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP10"/>
    <dbReference type="FunCoup" id="G3AP10">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP10"/>
    <dbReference type="GeneID" id="18873735"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61139"/>
    <dbReference type="eggNOG" id="ENOG502QPQM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030747_2_0_1"/>
    <dbReference type="InParanoid" id="G3AP10"/>
    <dbReference type="OMA" id="WSIYDAQ"/>
    <dbReference type="OrthoDB" id="5784at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070916">
        <property type="term" value="C:inositol phosphoceramide synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045140">
        <property type="term" value="F:inositol phosphoceramide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006676">
        <property type="term" value="P:mannosyl diphosphorylinositol ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03386">
        <property type="entry name" value="PAP2_Aur1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026841">
        <property type="entry name" value="Aur1/Ipt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052185">
        <property type="entry name" value="IPC_Synthase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310:SF11">
        <property type="entry name" value="INOSITOL PHOSPHORYLCERAMIDE SYNTHASE CATALYTIC SUBUNIT AUR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14378">
        <property type="entry name" value="PAP2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="7">
        <location>
            <begin position="190"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="422"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="428"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="445"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="474" mass="53532" checksum="B21445D488C3E806" modified="2011-11-16" version="1">MARILRLTMLPLRPIKLFQSYFLSEKAPTTTLPDLNTDTSINTSIRKLQHHQWSKGEILHYGFLTSVFSFVFIILPVSFLVKFPIAITFILCLLVPATSQFFLHALPVFAWLALYFTCSKIPNTWKPAISVQVLPAMETILYGDDLSNVLATINTRVLDIIAWLPYGILHFSLPFVVAGIIFLFGPPTSLRSFAFAFGYMNLFGVIIQILFPAAAPWYKNIYGLEPANYSMHGSPGGLGRIDALLGIDMYTTGFSNSPVIFGAFPSLHSGCSIMDVLFLCWLFPRFKPLWICYGCWLWWSTMYLTHHYFVDLIGGAMLSFVVFEFTKYKYLPVCDPTKFCRWSYTELKHININEIDPLSQNYIYQNDDESRLYTRIYPEQEPAVSGSYPLQPIIPTGPIVVPPIQEQVQESFEMATLRGVNEDDDEVSSNASNSPSVFENGPLYVSSNTSLDEMESSTSNGTSLPTVKKSVKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP28</accession>
    <name>G3AP28_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_50656</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375335.1">
        <property type="nucleotide sequence ID" value="XM_007375273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP28"/>
    <dbReference type="GeneID" id="18871468"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50656"/>
    <dbReference type="HOGENOM" id="CLU_1129674_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP28"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="246" mass="28388" checksum="833412D9D49728BE" modified="2011-11-16" version="1">MSLDATNRFGEFQNKIQQEIIQKWKTFNKSVKLEVMKQLQTQENQLDEMIQSIKDTGNEELGNLFEKLTTVKNNMQEAGKLQKSSLADIITILDKIQKEALDFDKFRKDHTVFVTDLNSLNIKLQNTIDKLRESQDKNRSKLKTPEKLEQSSVVSQNSKIMELENEKCIFQLKNSNQVLDIDKLLIPTSLDVVSSKTAMHISADDGRLEQKEKTSFCQTTSGKEVDDVYSFDNIIDFPPSKKYKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP31</accession>
    <name>G3AP31_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_61150</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375338.1">
        <property type="nucleotide sequence ID" value="XM_007375276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP31"/>
    <dbReference type="GeneID" id="18873745"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61150"/>
    <dbReference type="eggNOG" id="ENOG502SNTU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1017643_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP31"/>
    <dbReference type="OrthoDB" id="4076777at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="274" mass="32613" checksum="60F4E0C877130CDE" modified="2011-11-16" version="1" fragment="single">MEPDDRVTRVDPRSVRSINDMIRKTTTTIASPPPASASTNTDLEDHMQEENYIYIPSIKNYRTLDYAFDETKRVKREISSMQTSLINEFQEMLRRTFPTGFYMGLDLQCKSNQFSLIDSKTDARMELPMIPLYAKFVTTKRSKRRLWNHLFKLFLINVDEHIQNVIAVSEFQERQLDKFISRVKKNIKLAIQFRCDLKSSKIHVKRNVDAVLFKPYSDRNYTRIYWIKPPHANIGIRRGAMRVKWMNIEDVLNKKKPVTKHHQHQDQPHHYHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP38</accession>
    <name>G3AP38_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_50664</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375345.1">
        <property type="nucleotide sequence ID" value="XM_007375283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP38"/>
    <dbReference type="FunCoup" id="G3AP38">
        <property type="interactions" value="124"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP38"/>
    <dbReference type="GeneID" id="18871470"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50664"/>
    <dbReference type="eggNOG" id="KOG1305">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009020_4_1_1"/>
    <dbReference type="InParanoid" id="G3AP38"/>
    <dbReference type="OMA" id="FLFFGSQ"/>
    <dbReference type="OrthoDB" id="28208at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF458">
        <property type="entry name" value="SODIUM-COUPLED NEUTRAL AMINO ACID TRANSPORTER 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="142"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="264"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="392"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="415"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="458"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="10">
        <location>
            <begin position="68"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="500" mass="55053" checksum="A741BA4AD03CE58A" modified="2011-11-16" version="1">MSATVAHSIPPTQSDSKSGYLSVPQNAALHNGDTGDLTHEEFELQTVDSLDFEVEGEGSLEQPEETGRSNMKMAFMNMANSILGAGIIGQPFAFKNSGLVGGIVVMILLTLLIDWTLRLIVKNSILSQTKSYQDSVNYCFGLAGKILLLVSICSFAYGGCMAFCVIIGDTIPHVLKAFIPKSITGPGSPIAWLFYRNTIIILFTACISYPLSLNRDISKLAKASGFALVGMLIIVILTIARAPFVSSELRAKLTLPEWTVNYNIFQGVSVISFALVCHHNTMFIYNSMKNATLPKFAKLTHISCIVSMIFCMIMGINGLLNFGDKTKGNLLNNFRSDDNWINIARFCFGLNMLTTFPLEIFVVRDVLKEIILAKHASQGSIAELELSSRQHFFITTFLVFSSMSVSLFTCNLGMILELIGATSASLMAFIIPPLCYFKLSWQQQDFVNCSPKDKRRFIIYKALPSVGCVVFGVLVMFISSFMTISQAVRHKGEEGHCVID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP88</accession>
    <name>G3AP88_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">serine C-palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.50</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_72021</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.50" evidence="7"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375935.1">
        <property type="nucleotide sequence ID" value="XM_007375873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP88"/>
    <dbReference type="FunCoup" id="G3AP88">
        <property type="interactions" value="400"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP88"/>
    <dbReference type="GeneID" id="18875408"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72021"/>
    <dbReference type="eggNOG" id="KOG1357">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015846_7_2_1"/>
    <dbReference type="InParanoid" id="G3AP88"/>
    <dbReference type="OMA" id="QPRANGC"/>
    <dbReference type="OrthoDB" id="65434at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017059">
        <property type="term" value="C:serine palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004758">
        <property type="term" value="F:serine C-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06454">
        <property type="entry name" value="KBL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000047">
        <property type="entry name" value="serine palmitoyltransferase 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF3">
        <property type="entry name" value="LD36009P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="8 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="18">
        <location>
            <begin position="155"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW32659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="561" mass="62809" checksum="45753DD476D756B6" modified="2011-11-16" version="1">MQDKHYTKDIPNAPPDDRPKEVLADLEYGKLTSHDYLYVSKHPVGEPLPMPIDDEPPYIIYIATYISYLILIIIGHIRDFFGKIFKPKEYEDLIEKNGYAPWYDGFESFYVRRLKTRIDDCFARPIHGAPGRYIKCFNRIREGKTGYLYDGTSKECLNLSSYNYLGFAQSSGVCTDYALKITEEYGTSGCSPRTYCGTTDLHKRCEKIIADFAGKEDAIIVSQGYGTNANLFASLADSKTLVISDELNHASIRFGIRLSGALVKVFKHNDMNDLEKLLRNQISQGQPKTHRPWKKIIIAVEGLYSMEGNMCNLPKLVELKEKYKCYLFVDEAHSIGALGPEGRGVCDYFSIDPARIDLLMGTFTKSFGATGGYIAADKAIIEKLRINYITQSYSEVVPPPVLAQIISSLKIIKGELNPGEGQERLQRIAFNSRYLRLGLKKLGFIVYGADDSPVIPVLLFLPAKMPAVSRMLYEMGIAVVIVSYPATPLISARARLCVSSALTKEDLDYVLTKFNEIGDLIFLKFSSGIAGGSKEKGVPPRWSIEDVLATNVEDCKKPKLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP90</accession>
    <name>G3AP90_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61723</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375937.1">
        <property type="nucleotide sequence ID" value="XM_007375875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP90"/>
    <dbReference type="GeneID" id="18874022"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61723"/>
    <dbReference type="eggNOG" id="KOG2690">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_774258_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP90"/>
    <dbReference type="OMA" id="LFWYRYF"/>
    <dbReference type="OrthoDB" id="73788at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.20">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3970.10">
        <property type="entry name" value="BSD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051494">
        <property type="entry name" value="BSD_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019:SF5">
        <property type="entry name" value="BSD DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019">
        <property type="entry name" value="SYNAPSE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BSD" evidence="2">
        <location>
            <begin position="214"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="358" mass="41197" checksum="3AC68F18518A499F" modified="2011-11-16" version="1">MYTFDALVPEEPSQEESNEHVESKTEEAIEKLQEDIDKVYNSIQDKFQGLWSTTAKSAGEIQEKYKLDEYKQNLINQLNTTRESIKGSTPSAAISENLKSIEEQVKNIKLDGVKQELDVLSTQANHALDALDSQLEKVENVAGKYLSSFASYFTKNVISITPETTPQPEPAKEEVLFSSPLTNNYGTSRYDNDLFKLHTSKQYFLSNELDKAAEIEKFKADEKTKVISDLLEKYPNTLTKTMNELVPVEISYNLFWYRYFKNEDKLKDLEQKRKALLKEEEVELSEDKDEEEDEEFTWDDDEDFEEADDKLKESPVKVEPEDTKITTPTKPAQKTESNPAAKSEPEDADSEEEDDDWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3APE4</accession>
    <name>G3APE4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RanBD1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_71614</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375396.1">
        <property type="nucleotide sequence ID" value="XM_007375334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APE4"/>
    <dbReference type="FunCoup" id="G3APE4">
        <property type="interactions" value="229"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APE4"/>
    <dbReference type="GeneID" id="18875387"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_71614"/>
    <dbReference type="eggNOG" id="KOG0864">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4719">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018357_0_0_1"/>
    <dbReference type="InParanoid" id="G3APE4"/>
    <dbReference type="OMA" id="SDGMGHI"/>
    <dbReference type="OrthoDB" id="185618at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13181">
        <property type="entry name" value="RanBD_NUP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053074">
        <property type="entry name" value="NPC_Nucleoporin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015007">
        <property type="entry name" value="NUP2/50/61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38697">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN SIMILAR TO S. CEREVISIAE NUP2 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38697:SF1">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN SIMILAR TO S. CEREVISIAE NUP2 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08911">
        <property type="entry name" value="NUP50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RanBD1" evidence="9">
        <location>
            <begin position="653"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="166"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="363"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="580"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="629"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="193"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="255"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="274"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="303"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="473"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="597"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="639"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="784" mass="84199" checksum="CD8177A60000C876" modified="2011-11-16" version="1">MKIFFLPRLFICRLHPFFNYTNKSHDTPFSDVWEKEQITRDDFEKDHEAGDHDDIPTLAMKASDEVLATRKILKPKRRIDSSISNNIGFKGFGASAGNIPGNTFGFTKTDTTSSNDKNAKIRALNEQFVNAINQKNLPNSIVDYTPIAEKYIKYYKSITEGVSSVPAPKPQATADAPANPFAFKKTHTPVAGESSSLFSSQPKPTPSTAGQFTAPTTTSFQFEPKTETSKPFTFSAGASKPADATSVPKFVFPSKEPETKPQEPAPKKTSNEVIDIDSEPESDSDSEDDNMETSKPEIKVQGPQFTLSAKPSTKNSPFTFDPKKLAKINAPDSDDSDDEVVIKGPTFQFNKPIVDSVFKLGKSDNADKEKPAFSFGATANKEVAPSKPEPANDKPAFSFGSSAQKSDDKPAFTFGQTAEKKDDKPAFSFGQTAEKKDDKPAFAFGQTSEKKDDKPAFSFTPSEKKEDKPGLSFGSSQTTDKPSFSFKPNSEAKPFSFGSSAPAANDKPADKPAFLFGSTSAAPSETKDNKAAAFSFGSSSEAKPLFGSTTENKEEKPAAFSFGAKPAVSFGGEAPKFNFGGASSAFAKPTEDKQESSETTQKPLFNFAFNPSKNGSPFGGAAAAAAATTSSSAGTATEGGDDDKVPEEETGGDFTPVAQLSSEKMEHVSTGEEDEIALYTKRCKLMELDTSNTENPYINKGVGDLKVLKHKETQKSRILIRADGGLRVLLNTAIAKGIKYESMGNGSLVRIPTVDAAGKFVTFVVKVKTPADGSDLLKAITDVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3APG1</accession>
    <name>G3APG1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_71630</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375414.1">
        <property type="nucleotide sequence ID" value="XM_007375352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APG1"/>
    <dbReference type="FunCoup" id="G3APG1">
        <property type="interactions" value="1011"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APG1"/>
    <dbReference type="GeneID" id="18875390"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_71630"/>
    <dbReference type="eggNOG" id="KOG0733">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_8_4_1"/>
    <dbReference type="InParanoid" id="G3APG1"/>
    <dbReference type="OMA" id="GLWSTHR"/>
    <dbReference type="OrthoDB" id="27435at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19518">
        <property type="entry name" value="RecA-like_NVL_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19530">
        <property type="entry name" value="RecA-like_NVL_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000110">
        <property type="entry name" value="Ribosome biogenesis ATPase RIX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000365">
        <property type="entry name" value="Ribosome biogenesis ATPase RIX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="218"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="550"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="139"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="139"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="820" mass="91056" checksum="2CAD7A73DA4CB73A" modified="2011-11-16" version="1">MAKSSVVSGPIDQKIYNLIHDLLDEKTAENKEKLSGDAVQDPFTYMESANNLTITQVLSYVQSKDFQLKRMKKNQLEKTISTILKIVRDDEQEELGLGPPGEEVSGQESEADAETAHELMEVKDSNSLNKSVVAMWNAESKDGETDKEISSDSKDNKKRQKESSKSSTKRQKKKADYSPPNTNLASLGGLENITTQLLELIGLPILHPEIYTATGVEPPRGVLLYGPPGCGKTTIANALAGELQVPFINISAPSIVSGMSGESEKKLRELFEEAKTVAPCLIFMDEIDAITPKRDGGAQREMERRIVAQLLTLMDELTLDKTDGKPVIVIGATNRPDSLDSALRRAGRFDREICLNVPNEEQRASILRAMTKNIKLHDETQFKYKELSKLTPGYVGADLKSLVTAAGIAAIKRIFESLSEKHEETNLQENNVDSMEVDGTGADDLIDNQLLDNFSSKSDYEKLSTIKKFLDKHPNPLSKEQLEPLSITYEDFLQALPTIQPTAKREGFATVPDVTWQSVGALSNIRMELHMCIVQPIKKPELYLKVGITAPSGVLMWGPPGCGKTLLAKAVANESRANFISVKGPELLNKYVGESERAVRQVFQRARASIPCIIFFDELDALVPRRDSSLSESSSRVVNTLLTELDGLNDRKGVFVIGATNRPDMIDPAMLRPGRLDKTLYIELPSAEERLEILKTLVRANRTPLSKDVNLQEVANDMRCRNFSGADLSSLVKEAGIIALKKKFFKNQQIQELDASGFLEEDSPDSKVEIIMDDFNRALSSIKPSVSDRDRTKYEKLNQRMGWNIIKEETKEPSDPVNDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3APL5</accession>
    <name>G3APL5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Stretch-activated cation channel Mid1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_137955</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375462.1">
        <property type="nucleotide sequence ID" value="XM_007375400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APL5"/>
    <dbReference type="FunCoup" id="G3APL5">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APL5"/>
    <dbReference type="GeneID" id="18870086"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137955"/>
    <dbReference type="eggNOG" id="ENOG502QTEW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018731_1_0_1"/>
    <dbReference type="InParanoid" id="G3APL5"/>
    <dbReference type="OMA" id="YEILPCI"/>
    <dbReference type="OrthoDB" id="5405745at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024338">
        <property type="entry name" value="MID1/Yam8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39142:SF1">
        <property type="entry name" value="AEL197CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39142">
        <property type="entry name" value="MID1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12929">
        <property type="entry name" value="Mid1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442530" description="Stretch-activated cation channel Mid1" evidence="1">
        <location>
            <begin position="21"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="513" mass="57172" checksum="AA0131F53D28D368" modified="2011-11-16" version="1">MINLSLLLLLLLGCFQPVFSIIQIDNDTLSDFDLAQRITSNNKSTGLQEFTPIIDTIVQSDVKYYSFDVNTTSGLGEFYELLVFITGNICTQPAGIPENDTSLAVYYSFNSSMFEDNEYGQMSLFSDGYMQALADLPIGQVQTTEGYQPPTVLYIAVRAPENTNSSASWTYQIGVSQNDLVFQWDDREWVKVLDTDDTSAIVVTGNLSISEQYRRDKTMPYSLYVYSYGMKDYFTGLNNSWCAIRNGPALIGPDSIATSFTNSLDGTRQQFVVTGLNASTRYIGYVVAGSIGATTGGVVFHPFTFETMENDACQLIYGLEFCTQVAYSVPRPQGDPSVNITKQLYDNYTENLYTNFSKALQQIPCDAKPDSVFSNMKTCNDCANAYKDWLCAVMIPRCSTKNITGYQLREPNDGRNAFINDVVVPNKTYFEVLPCVNTCYAIVRDCPSDFGFLCPTKLPMSYFWDVDTGEGNEQWPSCNYVGKVVAIQSSRAFKLVLNYGVLLVLLLGSYSVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3APL9</accession>
    <name>G3APL9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_61274</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375466.1">
        <property type="nucleotide sequence ID" value="XM_007375404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APL9"/>
    <dbReference type="STRING" id="619300.G3APL9"/>
    <dbReference type="GeneID" id="18873811"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61274"/>
    <dbReference type="eggNOG" id="KOG0578">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_23_1"/>
    <dbReference type="InParanoid" id="G3APL9"/>
    <dbReference type="OMA" id="DTMKPLY"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06614">
        <property type="entry name" value="STKc_PAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000011">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF22">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SAMKA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="248" mass="28027" checksum="BF9D57FC64C9D850" modified="2011-11-16" version="1" fragment="single">LEQQPKKELIINEILVMKGSKHPNIVNFIDSYLVKGDLWVVMEYMEGGSLTEIVTHSVMTEGQIGAVCRETLKGLKFLHSKGVIHRDIKSDNILLNIDGNIKMTDFGFCAQINEINLKRTTMVGTPYWMAPEVVSRKEYGPKVDIWSLGIMMIEMIEGEPPYLNETPLRALYLIATNGTPTLKEPEALSAEIKQFLSWCLQVDFNKRATADELLQDKFILESDDVSSLSPLVKIARMKQAYGDNVEDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3APN0</accession>
    <name>G3APN0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Class II aldolase/adducin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61283</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375477.1">
        <property type="nucleotide sequence ID" value="XM_007375415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APN0"/>
    <dbReference type="STRING" id="619300.G3APN0"/>
    <dbReference type="GeneID" id="18873817"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61283"/>
    <dbReference type="eggNOG" id="KOG3699">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006033_1_2_1"/>
    <dbReference type="InParanoid" id="G3APN0"/>
    <dbReference type="OMA" id="NPWGLWW"/>
    <dbReference type="OrthoDB" id="3238794at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000009">
        <property type="entry name" value="Class II aldolase/adducin N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051017">
        <property type="entry name" value="Aldolase-II_Adducin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004855">
        <property type="entry name" value="PRK06208.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672">
        <property type="entry name" value="ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672:SF25">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 14 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="2">
        <location>
            <begin position="47"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="281" mass="30506" checksum="DF76E613F1324354" modified="2011-11-16" version="1">MSPTTADTTSTYDLGTRGTHNIAKGGKHPYALPKFTDKSEERKWALQHMAGAFRVFARKGYTEGTAGHISIRDPIDPDTFWINPLGKHFGLLKASDMVHVDEDGNVLPDGNQAPINAAGFAIHSALHKERPDVNAACHTHSTYGKAWSAMGKELDMINQDVCAFYKSHSVFSDFGGVALEAAEGIKIAKAIGDGKAAILQNHGLLTVGGTVDAAAYLFTLLEKSCQVQLSAQAALLPGESLKIIEDKVAEYCEYKDADPETLYTEFQPDLEMEIYLNGDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ97</accession>
    <name>G3AQ97_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Plasma membrane proteolipid 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_62008</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376222.1">
        <property type="nucleotide sequence ID" value="XM_007376160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ97"/>
    <dbReference type="FunCoup" id="G3AQ97">
        <property type="interactions" value="1254"/>
    </dbReference>
    <dbReference type="GeneID" id="18874149"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62008"/>
    <dbReference type="eggNOG" id="KOG1773">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107649_7_2_1"/>
    <dbReference type="InParanoid" id="G3AQ97"/>
    <dbReference type="OMA" id="GWGRECI"/>
    <dbReference type="OrthoDB" id="2802411at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659">
        <property type="entry name" value="HYDROPHOBIC PROTEIN RCI2 LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN LTI6 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659:SF42">
        <property type="entry name" value="UPF0057 MEMBRANE PROTEIN ZK632.10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01309">
        <property type="entry name" value="UPF0057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="57" mass="6425" checksum="5A51441B938BB0D0" modified="2011-11-16" version="1">MDSEKIIGVILAILLPPLAVFMKVGTTTPLWINIICCIFFWFPGILHALYVVLSDNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQD9</accession>
    <name>G3AQD9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62056</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376263.1">
        <property type="nucleotide sequence ID" value="XM_007376201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQD9"/>
    <dbReference type="GeneID" id="18874172"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62056"/>
    <dbReference type="eggNOG" id="ENOG502R1E6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_924913_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQD9"/>
    <dbReference type="OrthoDB" id="4083723at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000372">
        <property type="term" value="P:Group I intron splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025694">
        <property type="entry name" value="MNE1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13762">
        <property type="entry name" value="MNE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="301" mass="35627" checksum="F7B319EC95978388" modified="2011-11-16" version="1">MTLIDDAVSRSTKGEVIRYNDITQAINVKLMNYNVHDRNIERYNRGIQHRFEYYRSKVQESEETDPNNLLMVKKSNKNIYTAEQGVVLLNMSTLDEFVNLHMKYALDSKFHKYTRTFFINCLFAHLMTFGNFTALVNAMNGLSRVSRDWKGDLDYDSFKIIFTAMGNSESSKRCAYEFFTYFKRHSKKISRGYYIGMIKAASYGYVDYGVQFYIYHYLKDFGNLPESIIRLLPKSETMEQLVEFCSSRNKLTPEEIENYWATNNLNNSPDKLFREEENTNPQRYIPRYDERDVELLQKLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQK6</accession>
    <name>G3AQK6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_56379</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376331.1">
        <property type="nucleotide sequence ID" value="XM_007376269.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQK6"/>
    <dbReference type="FunCoup" id="G3AQK6">
        <property type="interactions" value="204"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQK6"/>
    <dbReference type="GeneID" id="18872029"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56379"/>
    <dbReference type="eggNOG" id="KOG0005">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010412_6_4_1"/>
    <dbReference type="InParanoid" id="G3AQK6"/>
    <dbReference type="OMA" id="YAGKQMA"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000211">
        <property type="entry name" value="Polyubiquitin 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="77" mass="8487" checksum="2A90D3C8D8969D3D" modified="2011-11-16" version="1">MQVKVKTLTGRDILVDVEPTDKIIRVKEMMEEKEGISPAQQRLIFNGSQLDDDKTIQESGISAGASLHLVLTLRGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQU7</accession>
    <name>G3AQU7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_51635</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376422.1">
        <property type="nucleotide sequence ID" value="XM_007376360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQU7"/>
    <dbReference type="GeneID" id="18871579"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51635"/>
    <dbReference type="HOGENOM" id="CLU_1310783_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQU7"/>
    <dbReference type="OMA" id="NINDDMW"/>
    <dbReference type="OrthoDB" id="4026651at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="210" mass="22831" checksum="B324AF43432EE696" modified="2011-11-16" version="1">MFNKLIGVTYIRNKLAHSNSTPSIPQEPSPSRTSTSSQRPRINSKAVSSSTDQVPKHKSKKHKKPTTINRAQVQQSIPSSMALAGRVRHSSSPVVPTIHTEFITALSPAQSRSSSASNSSSISYSAVEGSPTNANYITTNSHMFDGTDNFLFSHDLTIDMNLKKVNTNLSASGGTSLNATSNINDDMWSYNPEEGHTVYVSDKKKSVRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQV0</accession>
    <name>G3AQV0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_62260</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SKN1/KRE6 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376425.1">
        <property type="nucleotide sequence ID" value="XM_007376363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQV0"/>
    <dbReference type="FunCoup" id="G3AQV0">
        <property type="interactions" value="183"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQV0"/>
    <dbReference type="GeneID" id="18874235"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62260"/>
    <dbReference type="eggNOG" id="ENOG502QR13">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010811_4_3_1"/>
    <dbReference type="InParanoid" id="G3AQV0"/>
    <dbReference type="OMA" id="PYNYQYE"/>
    <dbReference type="OrthoDB" id="412647at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015926">
        <property type="term" value="F:glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02180">
        <property type="entry name" value="GH16_fungal_KRE6_glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000140">
        <property type="entry name" value="Beta-glucan synthesis-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005629">
        <property type="entry name" value="Skn1/Kre6/Sbg1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31361">
        <property type="entry name" value="BETA-GLUCAN SYNTHESIS-ASSOCIATED PROTEIN KRE6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31361:SF1">
        <property type="entry name" value="BETA-GLUCAN SYNTHESIS-ASSOCIATED PROTEIN KRE6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03935">
        <property type="entry name" value="SKN1_KRE6_Sbg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="11">
        <location>
            <begin position="269"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="24"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="78"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="695" mass="77250" checksum="BB9ADBA7F277275C" modified="2011-11-16" version="1">MARDLTSNTPVIPQEPVLDEDYVAQSNLNNPFEFTDSNEATSSYSSGSGGDSSSETPPDDNEPPRAPGYGERPFRAYSLSSSDPVSNNDSSSQEERKPLNPPRTMATPVTLPHRSYISDSSMNISGAPAAYDRYPTMGNGNVSRLSSSGSLQHFLPSKENSDTASSFTSNNYSRVPFDFSPFGGYPASSFPLHIDEKEPDDYLHNPDPIHDAIYDKNRLWNDLKNADKRSAWGLFGFAVLLAAAVAVFIILPVLTFANVEVHHSPQEYELLSNYQYPMLSAIRQTLVDPDTPDDALTITAKDGSQWVLVFSDEFNAVGRTFYEGDDQFFTAPDLNYAATRDLEWYDPDAVTTADGTLNLRMDAFNNHDLFYRSGMVQSWNKFCFTQGKIIMSARLPNYGTVSGLWPGMWTMGNLGRPGYLASTEGVWPYSYDSCDAGITPNQSSSDGISYLPGQKLNSCTCKGESHPNPGIGRGAPEIDVIEAAIGTDSTGKTPNIGVASQSLQLAPFDIWYMPDYSFIEIHNASVTTMNTYAGGPFQQALSGTTTLNSSWYEFGPYDHNFQRYAFEYLNDDNKGYLTWFVGEDPTFTVYPQALHPNGNIGWRHLSKEPMSIVMNLGISNSWAYIDWPSLNFPVTMRIDYVRVYQPKDQINVGCDPDDFPTYDYIQEHLNVYENVNLTLFEHGGYSFPKNKLTGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQY7</accession>
    <name>G3AQY7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP synthase subunit 5, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_61795</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase delta chain family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375994.1">
        <property type="nucleotide sequence ID" value="XM_007375932.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQY7"/>
    <dbReference type="FunCoup" id="G3AQY7">
        <property type="interactions" value="713"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQY7"/>
    <dbReference type="GeneID" id="18874051"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61795"/>
    <dbReference type="eggNOG" id="KOG1662">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085114_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQY7"/>
    <dbReference type="OMA" id="MVDNIQD"/>
    <dbReference type="OrthoDB" id="1262810at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.20">
        <property type="entry name" value="N-terminal domain of the delta subunit of the F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01416">
        <property type="entry name" value="ATP_synth_delta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026015">
        <property type="entry name" value="ATP_synth_OSCP/delta_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020781">
        <property type="entry name" value="ATPase_OSCP/d_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000711">
        <property type="entry name" value="ATPase_OSCP/dsu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01145">
        <property type="entry name" value="ATP_synt_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11910">
        <property type="entry name" value="ATP SYNTHASE DELTA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00213">
        <property type="entry name" value="OSCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00125">
        <property type="entry name" value="ATPASEDELTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47928">
        <property type="entry name" value="N-terminal domain of the delta subunit of the F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00389">
        <property type="entry name" value="ATPASE_DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="205" mass="21734" checksum="6619E6A4A87BC005" modified="2011-11-16" version="1">MASRVFARNLATATKSVKPPIQLFGLDGTYANALYSATVQESSVDQTYKSLGKVNDVIAKDAQLANALVNPSLSKENRVAVAGAISSKLGLDKTTGNFLKVLAENNRLGNFGSIYEKFGLLNDAHNGVVEAKITSAKPLDSKVLKRLTASIEKSSFVGQGKSLKITNLINSEILGGLVVEVGDKTVDLSISNKVARLNQALKEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARW9</accession>
    <name>G3ARW9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ER-derived vesicles protein ERV14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_62420</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cornichon family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376596.1">
        <property type="nucleotide sequence ID" value="XM_007376534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARW9"/>
    <dbReference type="FunCoup" id="G3ARW9">
        <property type="interactions" value="855"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARW9"/>
    <dbReference type="GeneID" id="18874315"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62420"/>
    <dbReference type="eggNOG" id="KOG2729">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112942_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARW9"/>
    <dbReference type="OMA" id="HKKECFI"/>
    <dbReference type="OrthoDB" id="434393at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003377">
        <property type="entry name" value="Cornichon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033466">
        <property type="entry name" value="Cornichon_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12290">
        <property type="entry name" value="CORNICHON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03311">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01398">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01340">
        <property type="entry name" value="CORNICHON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="15999" checksum="FF3CBBA7305D89B8" modified="2011-11-16" version="1">MNGESWLFILAVVVNAVNLFSQVFFTIMYSDLECDYINPIELCNKLNPWFIPEAGLHAFISILFLVNGYWFPFVLNLPLLAYNVNKFIGKNHLLDATEIFRTLSKHKKESFLKLGFHLLMFFFYLYRMIMALVNEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS09</accession>
    <name>G3AS09_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WKF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_62470</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376636.1">
        <property type="nucleotide sequence ID" value="XM_007376574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS09"/>
    <dbReference type="FunCoup" id="G3AS09">
        <property type="interactions" value="218"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AS09"/>
    <dbReference type="GeneID" id="18874333"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62470"/>
    <dbReference type="eggNOG" id="KOG4829">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067161_1_0_1"/>
    <dbReference type="InParanoid" id="G3AS09"/>
    <dbReference type="OMA" id="ISKWNTQ"/>
    <dbReference type="OrthoDB" id="10261563at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019327">
        <property type="entry name" value="WKF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22306">
        <property type="entry name" value="CHROMOSOME 7 OPEN READING FRAME 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22306:SF2">
        <property type="entry name" value="CHROMOSOME 7 OPEN READING FRAME 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10180">
        <property type="entry name" value="WKF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WKF" evidence="2">
        <location>
            <begin position="82"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="202" mass="23483" checksum="9A237E40F3881415" modified="2011-11-16" version="1" fragment="single">MSSTVPAWKRIGLSVKKDSGDDSLNIATTRVENADLTTKQIKKLSNKTKAQSQHKIEKKKPPKRIKLPKSERPPPPVKDQLTYLKQFETDRDNWKFSKQKQNWILKNIKNIPQEYETSLAVYIESLQGGSRDRLIPELKQVIEKWNVQYEEAERKIEEELAKKAEGSETSNEEKEKKESEPEKASEVNLEYAVRCFNLLKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS21</accession>
    <name>G3AS21_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">mRNA cap guanine-N(7) methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">mRNA (guanine-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">mRNA cap methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_155616</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Responsible for methylating the 5'-cap structure of mRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:67008"/>
            <dbReference type="Rhea" id="RHEA-COMP:17166"/>
            <dbReference type="Rhea" id="RHEA-COMP:17167"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:167617"/>
            <dbReference type="EC" id="2.1.1.56"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.56" evidence="3"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376648.1">
        <property type="nucleotide sequence ID" value="XM_007376586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS21"/>
    <dbReference type="FunCoup" id="G3AS21">
        <property type="interactions" value="1049"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AS21"/>
    <dbReference type="GeneID" id="18871023"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_155616"/>
    <dbReference type="eggNOG" id="KOG1975">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020346_2_0_1"/>
    <dbReference type="InParanoid" id="G3AS21"/>
    <dbReference type="OMA" id="KPFLEVW"/>
    <dbReference type="OrthoDB" id="10248867at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004482">
        <property type="term" value="F:mRNA 5'-cap (guanine-N7-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000280">
        <property type="entry name" value="mRNA cap guanine-N7 methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004971">
        <property type="entry name" value="mRNA_G-N7_MeTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039753">
        <property type="entry name" value="RG7MT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12189:SF2">
        <property type="entry name" value="MRNA CAP GUANINE-N7 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12189">
        <property type="entry name" value="MRNA GUANINE-7- METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03291">
        <property type="entry name" value="mRNA_G-N7_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51562">
        <property type="entry name" value="RNA_CAP0_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MRNA cap 0 methyltransferase" evidence="16">
        <location>
            <begin position="204"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="90"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="94"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW31870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="514" mass="59103" checksum="3FB4FB62D192A2CA" modified="2011-11-16" version="1">MDKTNMSNESTQESSKLIEPEISSASPEEPLAKRIKAKDDLPSEFSIRRRNSADTRQTTIASAYQYESEVPAWRKSDRGVDHKYDKYGSRQVKAAAAPSTPSTGATSDPRLQRIKRSRDELERSIDKIHQRNNRHQEEPEEEEEVNPYSNLDVANPSVNHQISNYHTFQSRITNRENKDINSIVRAHYNQRTQQSKFQGSRVNSPIYKLRNFNNAIKYMLLGNFVKHNREESEIFSFLDLCCGKGGDLNKCEFVGIEQYIGVDISDLSVREAYERYSKQKARLRNTRDGRHGSSRYNFEACFATGDCFTEFVPNILEPNFPGIINRAFPVDAVSIQFALHYAFESEEKVRTLLTNVTRSLRSGGAFIGTIPSSDVIKSKIVQKQYYKTEAGKCKFGNELYSVTFDKEPPADGVFRPPFGNKYRYWLKDAVDDVPEYVVPFETLRTLCEEYGLTLKYKKNFIDIFNQEIPRYFSKLNKHLIEGIKRSDGKYGAEGDEKEAVGFYIGFVFEKLQTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS65</accession>
    <name>G3AS65_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sorbitol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_62918</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377057.1">
        <property type="nucleotide sequence ID" value="XM_007376995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS65"/>
    <dbReference type="STRING" id="619300.G3AS65"/>
    <dbReference type="GeneID" id="18874525"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62918"/>
    <dbReference type="eggNOG" id="KOG0024">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_3_1"/>
    <dbReference type="InParanoid" id="G3AS65"/>
    <dbReference type="OMA" id="MRATTIH"/>
    <dbReference type="OrthoDB" id="3941538at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08284">
        <property type="entry name" value="FDH_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42813:SF2">
        <property type="entry name" value="DEHYDROGENASE, ZINC-CONTAINING, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G02810)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42813">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="5">
        <location>
            <begin position="25"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="4">
        <location>
            <begin position="192"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW31024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="362" mass="40016" checksum="6FCF64D880DA30BE" modified="2011-11-16" version="1">MKAVVYYGPYDIRTETRPIPQIQSPSDVIIKVKYSGLCGTDLHSYRGHIKGPVDTIIGHEFLGTISEVGKDVTKFKVGDDVLSTFTIQCGQCWYCKHGYSGQCDVTNTFGKVGLDGGQAEYVRVPYAENTLIKKPSSNDGVDDSIYVLMADIFITGYFGVKKIRDFFAQKSAEGVAIQQARDTTILQIGAGPVGLCGLRILKHFGFEKVVVVDGVDSRLQEATKLGAYKTVNYITNPDDLAKIITNDTNNIGFDAVLECVGAKSAQRTAYEAVRRNGFISSLGMGHDELPFNGLECYLKNINISFGRCHAWSLFAEALEIFEQLKSDFTDFIDYKASIDDSKSAFELFDKQKVKKVVFDLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASA9</accession>
    <name>G3ASA9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_141736</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376682.1">
        <property type="nucleotide sequence ID" value="XM_007376620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASA9"/>
    <dbReference type="STRING" id="619300.G3ASA9"/>
    <dbReference type="GeneID" id="18870396"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141736"/>
    <dbReference type="eggNOG" id="KOG2398">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_380874_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASA9"/>
    <dbReference type="OMA" id="RFAKSWN"/>
    <dbReference type="OrthoDB" id="27823at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120104">
        <property type="term" value="C:mitotic actomyosin contractile ring, proximal layer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF7">
        <property type="entry name" value="NOSTRIN, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="10">
        <location>
            <begin position="17"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="9">
        <location>
            <begin position="588"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="337"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="506"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="337"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="381"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="512"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EGW30649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="656" mass="75821" checksum="20BBE9D9B4CE627D" modified="2011-11-16" version="1">MTRTTLAPPRNGGGRESQFVNNFWGSGDYGYTTIQTRIKDSLRTMQELIDFYQEKIHVEKEYCKKLEKLSNKYPIGTHETGTLRKSLDKLNVENEHMIQNTYKFVKSIDEINLVKLKNFQGVYSKKVNKLQHHMNKVLMKKQDAWKHLEIVKNNYRQDCIKIKQFKLLQQTTWGKELEKHQRDYQKVNSSISTTRKNYQIALASYNEINDIYIKDWKISLNDYYKLELERIQTCKVNCFNYCNNIATLCVDNDQSVDLARTVFAQIQPPWDLQEFSNNYGTGDKIYQGCAFVEYMNGEEEKPIEYLLSNVNNPDHVPILSRTYSTYSSASHGVVHTNANNNHVTNTNNNPNTHVTKQLPPIAPLAPLDIAKPKTPSPTRKQPPEIETKSNVHQDVFSLKDRETHDSDEEDFARSNGSSNYSNPTTYSNYSTNSNNSEQRHWASPRRKEKQLQQVQEQITRRATNEFSNLMKKPIPNLSKPDTSPTKVPVIKDFSIDFIAKALEDLNSGGNGDVNQFRRSVRSQAESKRESYQTEPTTPVRPQSDFVDDRHETATRYESINFNSPVKKSTKSFLNLHAMINRTPANQKPFIGKARAKYSFTPQSDGELLFKKGWNMYIIYKQEDNWYVCELGNNCEDHEGLIGLVPGNYLIEGDDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASH8</accession>
    <name>G3ASH8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_142796</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377129.1">
        <property type="nucleotide sequence ID" value="XM_007377067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASH8"/>
    <dbReference type="GeneID" id="18870486"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142796"/>
    <dbReference type="eggNOG" id="ENOG502RA5R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2605803_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASH8"/>
    <dbReference type="OMA" id="IHKEIHY"/>
    <dbReference type="OrthoDB" id="4095573at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="79" mass="9121" checksum="C139D5F6B42F99B3" modified="2011-11-16" version="1">MSSVLSTGLKGLVAGLVIPPVVTKFVIIPYLFRNNFKEIHYIQHELDHVGWLVRGLEENKGYHEDDLYRPASYYGPYQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASM4</accession>
    <name>G3ASM4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial import inner membrane translocase subunit TIM14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Presequence translocated-associated motor subunit PAM18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_62573</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, it is required to stimulate activity of mtHSP70 (SSC1).</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterodimer with PAM16. Component of the PAM complex, at least composed of mtHsp70, MGE1, TIM44, PAM16, PAM17 and PAM18.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TIM14 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376743.1">
        <property type="nucleotide sequence ID" value="XM_007376681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASM4"/>
    <dbReference type="FunCoup" id="G3ASM4">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASM4"/>
    <dbReference type="GeneID" id="18874384"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62573"/>
    <dbReference type="eggNOG" id="KOG0723">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_13_3_1"/>
    <dbReference type="InParanoid" id="G3ASM4"/>
    <dbReference type="OMA" id="HPWITGM"/>
    <dbReference type="OrthoDB" id="240298at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000001">
        <property type="entry name" value="Import inner membrane translocase subunit tim14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763:SF28">
        <property type="entry name" value="GEO10507P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="15">
        <location>
            <begin position="48"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW30710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="105" mass="11428" checksum="82710E1E0A42680F" modified="2011-11-16" version="1" fragment="single">MGDHPWITGMGVLTLAYFGAGFIKSNQPGINGKAFIKGGFGQKMTAKEALQILNLKESNLSKAKLKEQHRKLMMANHPDKGGSPLLATKVNEAKDFLEKRGGMKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT04</accession>
    <name>G3AT04_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">AP-1 complex subunit MU-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_142843</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377198.1">
        <property type="nucleotide sequence ID" value="XM_007377136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT04"/>
    <dbReference type="FunCoup" id="G3AT04">
        <property type="interactions" value="601"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AT04"/>
    <dbReference type="GeneID" id="18870492"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142843"/>
    <dbReference type="eggNOG" id="KOG0937">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026996_0_0_1"/>
    <dbReference type="InParanoid" id="G3AT04"/>
    <dbReference type="OMA" id="KPLIWCD"/>
    <dbReference type="OrthoDB" id="3851628at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099638">
        <property type="term" value="P:endosome to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09250">
        <property type="entry name" value="AP-1_Mu1_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14835">
        <property type="entry name" value="AP1_Mu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000002">
        <property type="entry name" value="AP-2 complex subunit mu, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018240">
        <property type="entry name" value="Clathrin_mu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00314">
        <property type="entry name" value="CLATHRINADPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00990">
        <property type="entry name" value="CLAT_ADAPTOR_M_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00991">
        <property type="entry name" value="CLAT_ADAPTOR_M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="6">
        <location>
            <begin position="174"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="436" mass="50005" checksum="8BC361AED0C7B364" modified="2011-11-16" version="1">MASQIHFLDIKGKSLLSRDYKGDIPSNTIEKFPLLLLELENSIEEGEYKPFINNQGINYVFINHNNLYICALTRKNENIMAIIMFLSKLVEVMTQYFKSLEEESIRDNFVIIYELLDEMMDFGIPQITDTKILKEYITQDYYSLIKSSPQHLLTPPNAVTNAVSWRKDGIFYKKNEAFLDVIESINMLINANGQVLNSEILGEVKIKSHLSGMPDLRLGLNDKGIFTSEGDTSGKGIEMEDIKFHQCVRLSKFENEKIITFIPPDGEFTLMSYRLSSAQFLMKPLLLVNCRMKVHKHSRIEIVCSIKAQIKKKSTANNVEVIIPIPEDADTPKFNPEYGSVKWIPEKSCLVWKLKTFPGGKQFTMSAELGLPAVVDSEKAIANNKPIKVNFSIPYFTTSGIQVRYLRINEPKLQYQSYPWVRYITQSGEDYTVRMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT53</accession>
    <name>G3AT53_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_62680</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376848.1">
        <property type="nucleotide sequence ID" value="XM_007376786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT53"/>
    <dbReference type="STRING" id="619300.G3AT53"/>
    <dbReference type="GeneID" id="18874431"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62680"/>
    <dbReference type="eggNOG" id="KOG0393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_21_1_1"/>
    <dbReference type="InParanoid" id="G3AT53"/>
    <dbReference type="OMA" id="DNVASKW"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00157">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000118">
        <property type="entry name" value="Rho-related GTP-binding protein RhoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0488">Methylation</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="182"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="202"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="230" mass="25602" checksum="D2C7F234CCB636CD" modified="2011-11-16" version="1">MRSIKAVVVGDGGVGKTCLLISYTTNTFPNDYIPTVFDNYSASVMIDGEPIKLGLWDTAGQSEYDRLRPLSYPQTEIFLVCFSVVNPDSYEDVRTKWIPEIRHHCPRDVLILLIGTKIDLREDFHVLDELQARGINVITEAQGKKLARAIGAIEYLECSAATQVGVQEAFDYAIRAVLEPPSTDPVDSKVPNSGKSDHSVPAGQQRSGIFRRSMSNSESRRIKRKKCVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATB2</accession>
    <name>G3ATB2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Multidrug resistance protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_62781</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376908.1">
        <property type="nucleotide sequence ID" value="XM_007376846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATB2"/>
    <dbReference type="FunCoup" id="G3ATB2">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATB2"/>
    <dbReference type="GeneID" id="18874462"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62781"/>
    <dbReference type="eggNOG" id="KOG0255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008455_11_1_1"/>
    <dbReference type="InParanoid" id="G3ATB2"/>
    <dbReference type="OMA" id="AYLATCY"/>
    <dbReference type="OrthoDB" id="3357846at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015244">
        <property type="term" value="F:fluconazole transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="MFS_dom"/>
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        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
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        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
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        <property type="entry name" value="2_A_01_02"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FLUCONAZOLE RESISTANCE PROTEIN 1"/>
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    </dbReference>
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        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS_1"/>
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    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
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            <end position="184"/>
        </location>
    </feature>
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        </location>
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            <end position="247"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="506"/>
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        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="96"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EGW30875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="544" mass="60281" checksum="423B53FAF3FB6254" modified="2011-11-16" version="1">MVATLIRDSFLGRLTYQLSGHKFFTYPEEQPDYIIPEKYLSEGKAENFDKVEIENESSDVSVDTDDTKTANGDIIVTWDSDDDPENPQNWSIYKKTLVIIQVSFLTTSVYMGSAVYTPGVEALMEEFNISSVIATLPLTLFVLGYAVGPLVFSPLSENAAFGRNSIYIYTLALFVILQVPTALVHNIAGLCVLRFLAGVFASPCLATGGATVADVLTPPYIPVGLASWALGAVCGPSFGPFFGSILVVKGGWRWTFWFMCVLSGFCLFFLSWSFPETYGKTLLYRKAIRLRGITGNQRITSEGELENKSKTTNEIIVETLWRPIEVFIKEPVVLMINIYIALVYSIFYLFFEVYPIFFQEIRGFTLIELGCTYFATVIGIVIAAAIYIPVVRQIYTKRIVNGGSVQPEVFMPIAIFGAVCMPIGLFIFGWSATRHTHWIGPLIGGAIFSAGGFLIFQTLFCFIAGSFTYVGSIFASNNLFRSTTAGCFPLFGRPLYDNLATKEFPVAWGTSILAFLALAMIAIPVLFYLNGPKLRARSKYALQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATG7</accession>
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        <name evidence="6" type="ORF">SPAPADRAFT_142491</name>
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            <taxon>Saccharomycotina</taxon>
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        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="OMA" id="AWFYGSE"/>
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    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
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    </dbReference>
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    </dbReference>
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            <begin position="20"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="325" mass="36640" checksum="9FA494814098E805" modified="2011-11-16" version="1">MKFATTVELKLSNGKYIPALGLGTIPSGDASDVKDQVITAIKAGYRHIDTAWFYGSEKYIGEALKEVFQQGIIKREDLFITTKVWPSFWHSPEKSLDISLKDLGLDYVDLFLQHWPVALHGDQNGQPAVPKNEDGSLKYDDDPVHGTKFIDTYHKLEDILENTTKTKSIGISNYSIPKLKQLLPKIKKHRPVVNQIELHPQLPQRDLVDYCESRGIVIVAYSPVGGPGAPVLEVPLIKELATKYDVSPNEIAEAYHILNGRVALSRSSNLSRIKTITRLPRITEDELEQLYQVGVKDPKRYTCDPWGYGLGFKYWKGDTLSTEFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATS2</accession>
    <name>G3ATS2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_143220</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377270.1">
        <property type="nucleotide sequence ID" value="XM_007377208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATS2"/>
    <dbReference type="FunCoup" id="G3ATS2">
        <property type="interactions" value="557"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATS2"/>
    <dbReference type="GeneID" id="18870527"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143220"/>
    <dbReference type="eggNOG" id="KOG1441">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019048_4_1_1"/>
    <dbReference type="InParanoid" id="G3ATS2"/>
    <dbReference type="OMA" id="FWYTVSS"/>
    <dbReference type="OrthoDB" id="1588579at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="262"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="376"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="399"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="7">
        <location>
            <begin position="98"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="60"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="423" mass="47312" checksum="5B05C26FC10FA27F" modified="2011-11-16" version="1">MKQSFYASSQINPNFSSTNLSKINSPAPNRTSSYGPTPPLSRNSSIASFQQQSSPKRGFSLLSPPVSKPSSPEPVRGTANTTTTTPSKWSWLPPIDYKVTALCVAWYFTSIISSNSTKLILTNYPYPVTLTQFQFLLNSCLCIVMLAILGVKRNWVENLPSGVLPESLDIKSLITPTSLIINTTLPMGCFQFIGHLTSHKATSLIPVSLVHTIKSLSPIMTVFIYRALYNKKFPQRTYITLLPLIMGIMMTCYKPSSTSHISGYSTGLLFALMSMIIFVSQNMFAKKRLTIESDLPMAKQTQKKVDKLTILFYCSMIGFVLTSPIYLMSEVFNQSVSLFQLDSYVITMVLLNGISHFIQSLLAFQILGMISPINYSIANILKRIFIILVSFIWESKQFTSLQSIGLLITLFGLYAYDRWGTQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATW2</accession>
    <name>G3ATW2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Very long-chain fatty acid transport protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Very-long-chain acyl-CoA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">SPAPADRAFT_143042</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Acyl-CoA synthetase required for both the import of long chain fatty acids (LCFAs) (C14-C18) and the activation very long chain fatty acids (VLCFAs) (C20-C26) by esterification of the fatty acids into metabolically active CoA-thioesters for subsequent degradation or incorporation into phospholipids. The transport and fatty acyl-CoA synthetase activities are genetically separable and are thus independent activities. Esterifies VLCFAs in the peroxisome matrix. The VLCFAs are actively transported into peroxisomes by a PXA1-PXA2 heterodimeric transporter in the peroxisomal membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a very long-chain fatty acid + ATP + CoA = a very long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54536"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58950"/>
            <dbReference type="ChEBI" id="CHEBI:138261"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377309.1">
        <property type="nucleotide sequence ID" value="XM_007377247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATW2"/>
    <dbReference type="FunCoup" id="G3ATW2">
        <property type="interactions" value="132"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATW2"/>
    <dbReference type="GeneID" id="18870510"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143042"/>
    <dbReference type="eggNOG" id="KOG1179">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_46_3_1"/>
    <dbReference type="InParanoid" id="G3ATW2"/>
    <dbReference type="OMA" id="VWRQFLD"/>
    <dbReference type="OrthoDB" id="10253869at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031957">
        <property type="term" value="F:very long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044539">
        <property type="term" value="P:long-chain fatty acid import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000038">
        <property type="term" value="P:very long-chain fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000020">
        <property type="entry name" value="Long-chain fatty acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000019">
        <property type="entry name" value="Long-chain fatty acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107:SF15">
        <property type="entry name" value="FATTY ACID TRANSPORT PROTEIN 3, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="13" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="21">
        <location>
            <begin position="95"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="22">
        <location>
            <begin position="526"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="215"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="223"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00068795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="EGW30338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="653" mass="74471" checksum="1CD9036EE25EF7F0" modified="2011-11-16" version="1">MSGLEIALGAFLGTQLLESKYLIKDDIMLIQKVVRSALPYLYRSNHGRASYWYYFEQAALKYGNRKAVCFPRPVANSEEPPLDADGFPIYDTQFQIEEYTYKQLYDMVLRMSYIMKYEYGITSEHTIGIDCMNKPLFLVLWLALWNIGALPAFLNFNTKDKPLIHCISIANVSQVFIDPDCADPIRETEDKIKQELPNVKLNYLDEEKLFDRLSRKSTPKHRAKDETRRPQDKDSSPAALIYTSGTTGLPKAGIMSWRKAFMASIFFGYIMKVNKDSNVLTAMPLYHSTAAMLGVCPSLITGACVTLSQKFSASTFWTQARLSKATHVQYVGEVCRFLLHAQYHPDQVRHSVKIAYGNGLRRDIWGQFKKRFHIEAIGEFYAATESPIATTNIQYGEFGIGACRKYGSLINLFLRYQQTIVKMDPEDENEIWRDPKTGFCVRAGPDEPGELLMRILNPKKIEETFQGYYGNKKATSSKLITNVYSKGDAWFRSGDLLKMDSDGLLFFVDRLGDTFRWKSENVSATEVENELMGSNAIKQSVVVGVQVPQHEGRACFAVVEPVDEIDDQTVLTKIRDHVVHSLPNYAQPQFIKIARNNEIESSHNHKVSKNQFKKQKLPRGESGEEVIYWLNKTRYEQLDDQSWASICSGKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU00</accession>
    <name>G3AU00_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_68480</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377347.1">
        <property type="nucleotide sequence ID" value="XM_007377285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU00"/>
    <dbReference type="STRING" id="619300.G3AU00"/>
    <dbReference type="GeneID" id="18875221"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68480"/>
    <dbReference type="HOGENOM" id="CLU_474994_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU00"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002395">
        <property type="entry name" value="Kininogen"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00334">
        <property type="entry name" value="KININOGEN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442372" description="Hyphally-regulated cell wall protein N-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="288"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="344"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="375"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="455"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="478"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="529"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="574" mass="60835" checksum="D2D090C4790EA829" modified="2011-11-16" version="1">MRLIIAITTWFLSGAIALDVSSTVTYTSTSVFFEPLHVSGILTLLDTVLFAAIPFTIDALAYFFLRNTSPSTIRTSDMVNNGYALFDVSGTNSVYIPFQNSNQNNGEFIVYGQHLDVAMYGVENDGSMTFWGDTGSVSIDGITNAGSICFHNQLGMLYRPGGKGSYTLSQSTVFANSVQSDFDPRITFEGSGNGFVNQNGDPALHYTLVNFGGSNYVSVPNQGYTRYTYSESDGIFTMFMSSNQIVFDIGLGYINGFNITITNAAPGTANQGGSVCELNLNPFGGGSTSSVEGSSSSEAVEPTSEESTEIETTSDEVETSTEVVTSTSDIEESTQGDTTIDEATSVVTTTDIDTTTPVVEESTTPEPASSEEETTSAVSSVTDSSSVGSSSSSTPRPPSCKKVNGKWKGNCPGHGHDNGNGHDHRPGKGHGNDHNNGHGNDHNNGHGNGHNNGHGNDHNNDHNNGHGNDHNNGHDNGHGNNNNGHNNGHGNDHGNNNNKGNNKNNNYDNGNGHGNDHDNKNGNSNGKGKGNDNKDNNGHGKGHKREAIQENSTSSANKRISMLLVIALFLTVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU12</accession>
    <name>G3AU12_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein RPL32</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RPL32</name>
        <name evidence="4" type="ORF">SPAPADRAFT_57180</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377360.1">
        <property type="nucleotide sequence ID" value="XM_007377298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU12"/>
    <dbReference type="FunCoup" id="G3AU12">
        <property type="interactions" value="1012"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU12"/>
    <dbReference type="GeneID" id="18872098"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57180"/>
    <dbReference type="eggNOG" id="KOG0878">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071479_4_0_1"/>
    <dbReference type="InParanoid" id="G3AU12"/>
    <dbReference type="OMA" id="GPHNTAK"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00513">
        <property type="entry name" value="Ribosomal_L32_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001515">
        <property type="entry name" value="Ribosomal_eL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018263">
        <property type="entry name" value="Ribosomal_eL32_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036351">
        <property type="entry name" value="Ribosomal_eL32_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L32 AND DNA-DIRECTED RNA POLYMERASE II, SUBUNIT N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01655">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01393">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52042">
        <property type="entry name" value="Ribosomal protein L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00580">
        <property type="entry name" value="RIBOSOMAL_L32E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="140" mass="15979" checksum="56E5A66CA35772F6" modified="2011-11-16" version="1">MLKNHPTLQMAASLPHPKIVKKYTKKFKRHHSDRYHRVAENWRKQKGIDSCVRRRFRGTIPQPNIGYGSNTKTKHMTPSGHKVFLVKNVKDLDVLLLHTKTYAAEIAHNISAKNRVEIVAKAKKIGVKVTNPKGRVSLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUE9</accession>
    <name>G3AUE9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sec20 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_73229</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SEC20 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377496.1">
        <property type="nucleotide sequence ID" value="XM_007377434.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUE9"/>
    <dbReference type="FunCoup" id="G3AUE9">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUE9"/>
    <dbReference type="GeneID" id="18875473"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_73229"/>
    <dbReference type="eggNOG" id="ENOG502S7WD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051527_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUE9"/>
    <dbReference type="OMA" id="WSFFKIF"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005606">
        <property type="entry name" value="Sec20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056173">
        <property type="entry name" value="Sec20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12825">
        <property type="entry name" value="BNIP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12825:SF0">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SEC20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03908">
        <property type="entry name" value="Sec20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Sec20 C-terminal" evidence="11">
        <location>
            <begin position="151"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW30525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="335" mass="38501" checksum="A377CB875801448B" modified="2011-11-16" version="1">MSSHVYYKKLDNLQHQIYTSLDSLTTTTQDFDSTVNKHLHQQLLKYKDYLRICKHKLATSEDFHTFELYQLRYSSFKKKLREILMDNHSREAQLSHEFRIKQFGLDKPVQSEKSNTSSGLSDRDQLFGDAKSRETNKAISELSMNHQIAHHNKQITQSLQTSRQLLSATILQSELNLNSLDQQTKDLHKLNEGFVQFSSLLNRSQQIVKFIEKQDKADKQRIYMSGAFFLSCCCWVIYRRVLRTPIRLMLWSFFKIIGIYGWLTGGKDTSVVLSSGIMETVATALESSLEETVSEQAITSLASSAIIATTILSSLLDSEVYTDSSTYIEPEHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUM7</accession>
    <name>G3AUM7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_63421</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377554.1">
        <property type="nucleotide sequence ID" value="XM_007377492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUM7"/>
    <dbReference type="GeneID" id="18874742"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63421"/>
    <dbReference type="eggNOG" id="ENOG502S16T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034208_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUM7"/>
    <dbReference type="OMA" id="FYPPTNI"/>
    <dbReference type="OrthoDB" id="4095076at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="428" mass="49918" checksum="351FDC0F1FF5C7C9" modified="2011-11-16" version="1">MEPTSSLKYVNSHFYLLYNDLFYNKIISVFGEDIIGILVKILPWLKPYIKSLDTFRYDARMVVASRLGLVDNGVSEVGNPLNCMFVKDSWKYVYSVLKNKRLYAEYSDYRIDEPSNYVYNHFVEINRTYLLSYSKAIWLPPGNYNLNIGLAVKHGSGLGTTKFEIKYANEDGKVVVQTFYPPTNINEILPKNQFCFLKIGEFKVPPIQPKRADLEGRVQLLPKLRKVQLTMEEIGLYLKSGFSIYFIDISQPTLLYNDYDLMYYTVKETNYKYFVNILLKNLYKALNFVQNGGCPDDPNIQCVPNYYGGGDPFVISNRYHKLQDCTGDSELSQDSVSLPGTFSGYDEKLLMKYADFYYNSKFKKRYFKFTTIYQRRQFINRFGDFEMDWNDSNEEEGDSKSPEALTHVRRCSYDKEGLKWKIPIVGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUM9</accession>
    <name>G3AUM9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribosomal protein S2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_63428</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377556.1">
        <property type="nucleotide sequence ID" value="XM_007377494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUM9"/>
    <dbReference type="FunCoup" id="G3AUM9">
        <property type="interactions" value="779"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUM9"/>
    <dbReference type="GeneID" id="18874744"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63428"/>
    <dbReference type="eggNOG" id="KOG0832">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040318_4_3_1"/>
    <dbReference type="InParanoid" id="G3AUM9"/>
    <dbReference type="OMA" id="RNCINEC"/>
    <dbReference type="OrthoDB" id="2320368at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_B">
        <property type="entry name" value="Ribosomal_uS2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005706">
        <property type="entry name" value="Ribosomal_uS2_bac/mit/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01011">
        <property type="entry name" value="rpsB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S2 PROKARYOTIC AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="408" mass="45627" checksum="4BB2FA88A280D92F" modified="2011-11-16" version="1">MSQKELRNKEIQEALALEEEAFQRSKEIRELKFQTVEMISSLNKTPSTLINSRLQKLQQDLDNLPADKVKQLDEELQEFMNSQVALPTIDSINRPWVSKNSSDDSSSRLVPTQSKFGSYPNLRTTPDYKGYSDQELYLRQLNHARLCGKAGSKLSNVYRPHHDIRYPPSVNDTTIANLMAAGCHFGTSKQSWRESTQPFIYGEYNGIHLIDLNVTIERLKYACNVIKGVAEKGGIIVYVGTHKNTSVQNALIAAAKKSKGYYVSKRWIPGIISNFIEVTGQIKKEIMVEVDMEDNLTGNNDLRFHGKVLKPDLVVLLNPLENRNCVNECAASNIPTVGLCDTDMEPSLLTYPIPCNDDSIRAVTLMLGILSRAAEDGMNARLNAINEHKEAQAEIAKAINSSEKAAIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUP9</accession>
    <name>G3AUP9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_68679</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377576.1">
        <property type="nucleotide sequence ID" value="XM_007377514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUP9"/>
    <dbReference type="GeneID" id="18875234"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68679"/>
    <dbReference type="eggNOG" id="ENOG502TDFT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084555_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUP9"/>
    <dbReference type="OMA" id="YEKYIIR"/>
    <dbReference type="OrthoDB" id="4023279at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="287" mass="33278" checksum="97168BFBC68EA879" modified="2011-11-16" version="1">MTEQTPNQSILSPANYYFLLKTENIQPLLETVDQLQHEINENHATIKRLSQIIDYKLNGFPELKLDEDVIRLADSYSGNSNDNGHVSVDEATAGRQRELYSMNEDQENEEEEEEEEEEDNEKDTEPESIDPLRYLLEQKYQLPPPIPESSATDPTKLRMQQLLADISHLSKLQRGKVYRNSQLLEIIQEYEEFIMTNLLPQLREQLHGLVYDQEISNIKHMVDKKHECVEKIYKKYLNNVQGLCKIIDLNKAVIKFMDVEDGFNDKLSHQLASLEALKSNLIGKSDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUX4</accession>
    <name>G3AUX4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_52901</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom22 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377832.1">
        <property type="nucleotide sequence ID" value="XM_007377770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUX4"/>
    <dbReference type="FunCoup" id="G3AUX4">
        <property type="interactions" value="280"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUX4"/>
    <dbReference type="GeneID" id="18871733"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52901"/>
    <dbReference type="eggNOG" id="KOG4111">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094333_2_0_1"/>
    <dbReference type="InParanoid" id="G3AUX4"/>
    <dbReference type="OMA" id="LVWIVTT"/>
    <dbReference type="OrthoDB" id="196717at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22884">
        <property type="entry name" value="TOM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005683">
        <property type="entry name" value="Tom22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12504">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12504:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM22 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04281">
        <property type="entry name" value="Tom22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW30066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="144" mass="15951" checksum="1857131F57D02208" modified="2011-11-16" version="1">MVKLTQIDDETQAQFEEKPASTSIVDSEEESESESDDEFDDDYDFENETIYDRIVALKDIIAPEQREQLVSVTETIKSTVNATLNNGGKFLWTLTSSALLLGVPLALAILSETQLQEMEKDLSLQQSAQDVLAPGSEAAFSEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVE4</accession>
    <name>G3AVE4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_63770</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377929.1">
        <property type="nucleotide sequence ID" value="XM_007377867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVE4"/>
    <dbReference type="STRING" id="619300.G3AVE4"/>
    <dbReference type="GeneID" id="18874908"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63770"/>
    <dbReference type="eggNOG" id="KOG0773">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066267_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVE4"/>
    <dbReference type="OMA" id="NQLMMAT"/>
    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="6">
        <location>
            <begin position="193"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="195"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="56"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="168"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="266" mass="29901" checksum="A7DB7EBD3FF14BC8" modified="2011-11-16" version="1">MHLNEIINEGPSPPRPVVLPTVSPSSNISLPPISDILSISQLPPVSALQQTATAVQYPKIQQQQQQQQPPPPPPPMPMSAPPAPPGAVPSQYALPSITQQQMVMTPIPPQSLGTPTNTNYKYYYDYYPSESTLDRSPISHHASPVFYTTSQAGAIGLPSLQVQPFLRHNSYPCKDNHRRRNDDSDDGSISPPPIKRKTRNNLPKEITFILLKWLNDHLNHPYPNSFEKNQLMMATGLNQQQLSNWFINARRRKIKSLKEQKRLNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVQ5</accession>
    <name>G3AVQ5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Telomere length regulation protein conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_57650</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TEL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377986.1">
        <property type="nucleotide sequence ID" value="XM_007377924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVQ5"/>
    <dbReference type="FunCoup" id="G3AVQ5">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVQ5"/>
    <dbReference type="GeneID" id="18872141"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57650"/>
    <dbReference type="eggNOG" id="KOG4346">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017275_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVQ5"/>
    <dbReference type="OMA" id="FPLLNVW"/>
    <dbReference type="OrthoDB" id="10258062at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051083">
        <property type="term" value="P:'de novo' cotranslational protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.720">
        <property type="entry name" value="Telomere length regulation protein 2, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038528">
        <property type="entry name" value="TEL2_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051970">
        <property type="entry name" value="TEL2_Regulation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019337">
        <property type="entry name" value="Telomere_length_regulation_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15830">
        <property type="entry name" value="TELOMERE LENGTH REGULATION PROTEIN TEL2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15830:SF10">
        <property type="entry name" value="TELOMERE LENGTH REGULATION PROTEIN TEL2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10193">
        <property type="entry name" value="Telomere_reg-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Telomere length regulation protein conserved" evidence="3">
        <location>
            <begin position="465"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="440"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="445"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="830" mass="95072" checksum="5989EA779107C904" modified="2011-11-16" version="1">METNIEVLKNYPNIDKVNGILIDVLSTNKNPPLQLISTLLNYTIPQIFSSLPNEVHINLIKVFQSLIGISNLVNVIAVFKDGSHENKDVLQIYWQFLQELIDDKLVVELIRTSSSPLEIKEIDKFFFKGKMFSIVNEALISNNLEVDNKALESTANYVYYLTSSLLKLYQNKCDLIITTNYMNSILKFSGGESFNQFFDVFFTRENWGYFLDSFALMKKFEKKNLIKKFYTLYISKIHLQPNVSPEKLVGLYNILNFSHEYLDSSTVESIILCGNRDLNKLVAMVLNDCPETQLNSLIFQLLSSWSSEESVNNEPIAIQESRTHILLQILSRRKGSEFLKQLVKSKVFLDAITKRLSSFSDNVKALGVAVADHVCILNGEEKIFKFNDSVDNYSNLVESVLEIRTMGQSEAWNNIKAPYVEEEEKEEITTISSEISNLSVQQVNSDDESEDDDPSIPPQVDVQSPVYIKDLLQYLNVDTKDNNAYEMRRKALTEGPTLLRRKSRSGNEVQFFSEDLFSSLLALNNHFNDKDFEVLKLNNLIAVIVANPNITFYVFKLLLTGDYSLQQRMQILSATSLAARELRGFKDDVVTKSFKQINFPSQMLPESLHNKYMQLEADNTKYIDNIHTELQNSLMEEASSKAQDEILGKGKLVRISAKLKKQPKQQADPPVISNYFKIIATNFYFPLLNVWYEAGSIDIGHYSPIFIAHYIKTLVLIIHCSYPSSTQLRDMVKEFLILSCSIIRRLSSDQLQLIESIVTGILLIFDIIDGQYLISYHHDELILIQSWLMITWETLIDEKVKSLCAGLLLQISELLQTFERTLIDQTNSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVX0</accession>
    <name>G3AVX0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_68858</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377781.1">
        <property type="nucleotide sequence ID" value="XM_007377719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVX0"/>
    <dbReference type="GeneID" id="18875244"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68858"/>
    <dbReference type="eggNOG" id="KOG3017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035730_10_0_1"/>
    <dbReference type="InParanoid" id="G3AVX0"/>
    <dbReference type="OrthoDB" id="337038at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442656" description="SCP domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="2">
        <location>
            <begin position="91"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="244" mass="27014" checksum="05955F0A7764FE8A" modified="2011-11-16" version="1">MKLSAAITLAYLALFAQSVVLPDENAEAPIVAVAERSIEEEAGIVDIAERSVQEEADILAAAQNSVLQAMEGEVGSGELSKRLFGLSFDLGFSINILTFHNFKRILHGDPLLCWDHGLYRHAKHYIDRYDCSGDFHHPDGPYGSNWGIGSSKAFSVSADAGFVVDKWYGLGKSIGFGKSISLGAKVSLNSFTNLIWKSTRRVGCAYKDCRHRGRGYFYYCNYDPCANNPSYRVVQSNIPPRRNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADW0</accession>
    <name>G3ADW0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arginine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Arginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_57732</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Arg) + L-arginine + ATP = L-arginyl-tRNA(Arg) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="2"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372096.1">
        <property type="nucleotide sequence ID" value="XM_007372034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ADW0"/>
    <dbReference type="FunCoup" id="G3ADW0">
        <property type="interactions" value="1028"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ADW0"/>
    <dbReference type="GeneID" id="18872173"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57732"/>
    <dbReference type="eggNOG" id="KOG1195">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006406_6_2_1"/>
    <dbReference type="InParanoid" id="G3ADW0"/>
    <dbReference type="OMA" id="YEFKWER"/>
    <dbReference type="OrthoDB" id="68056at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07956">
        <property type="entry name" value="Anticodon_Ia_Arg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000006">
        <property type="entry name" value="Arginyl-tRNA synthetase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000058">
        <property type="entry name" value="Mitochondrial arginyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956:SF11">
        <property type="entry name" value="ARGININE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="ARGINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DALR anticodon binding" evidence="11">
        <location>
            <begin position="372"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW34684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="490" mass="56093" checksum="A80039A6F8783E08" modified="2011-11-16" version="1">MNPSLLFNLVINDVLKRKTDYGYLPLGVGKKVIVEFSSPNIAKPFHAGHLRSTIIGGFLSNLYEHAGWNVTRINYLGDWGKQFGLLAVGFDRYGSEEKLAQDPINHLFEVYVKINKDVTDEGENSKTNDEARAFFRKMEDGDEASLKIWKRFRDLSIDKYIDTYARLNIKYDVYSGESQVPKELMKKATDMFEEKGLIHVDRGAKLIDLTRFNKKLGKTLVEKSDGTSLYLTRDVGEAIKRYETYKFDKMIYVIAAQQDLHCAQFFEILKQMGFEWAKNMEHVNFGMVQGMSTRKGTVVFLDNILQETKEKMHEVMKKNEEKYAQIENPDKIADLVGISAVMIQDMQSKRINNYEFKWDRMTSFEGDTGPYLQYAHSRVCSVQRKADHISADDLVNANYELLTEPSAQALIRTLAQYPDTIKKALKTLEPSTIVTYLFNVSHIISQCYDDLWVAGQPDDLAAARLALYVSAKQVLYNGMTLLGLTPVERM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADZ8</accession>
    <name>G3ADZ8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Kinetochore protein mis13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_47807</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372135.1">
        <property type="nucleotide sequence ID" value="XM_007372073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ADZ8"/>
    <dbReference type="FunCoup" id="G3ADZ8">
        <property type="interactions" value="438"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ADZ8"/>
    <dbReference type="GeneID" id="18871146"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_47807"/>
    <dbReference type="eggNOG" id="ENOG502S2VJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043824_0_0_1"/>
    <dbReference type="InParanoid" id="G3ADZ8"/>
    <dbReference type="OrthoDB" id="3364649at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000444">
        <property type="term" value="C:MIS12/MIND type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013218">
        <property type="entry name" value="Dsn1/Mis13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14778">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN DSN1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14778:SF2">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN DSN1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08202">
        <property type="entry name" value="MIS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="277"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="59"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="455" mass="52744" checksum="966494FEF970B32A" modified="2011-11-16" version="1">MPAKKKKPTRKQQQLPVSSKRVISGEPIPNLSPPSSLPQQQSTTKEFPLYTRPSKYSALFNSQDSTTASYSEITTKRTSQSQPIEHNGRKKRKKQWSTPIQQLQQEINLLGNDEDDEDDDETVEFKFTVDKPTTKTNNFLDGVSQHMVGLPDEAPRKSINKRRQSYNNRGKRASSIGNGFITDLHEDVPLKNYYKHINASLPDPDRMRQLLVWCSKRRLAQDNNKKESNTNNQTNLNIAKVIEEEITKSLMDKRIPTSWYNRENDPSQVYGGPKVMMENLQNQKNFANNKIFSEKVNKLKKGIRQWEQEYNSDVDAIRKTPTDVPRDIKKKQIDDYSSNTNLEIDTSIVDKLTESYSQDAQLVNKDLQYYLEILNHTVRLLHIDAKKAQLIQEEHAINPVQSYKDDFYRRNSIENYRALRAREDKSSVHWPYPIASLKTKDLLTCLGIIQTRNKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE37</accession>
    <name>G3AE37_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_146959</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372983.1">
        <property type="nucleotide sequence ID" value="XM_007372921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE37"/>
    <dbReference type="STRING" id="619300.G3AE37"/>
    <dbReference type="GeneID" id="18870736"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_146959"/>
    <dbReference type="eggNOG" id="KOG1716">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011664_0_0_1"/>
    <dbReference type="InParanoid" id="G3AE37"/>
    <dbReference type="OMA" id="KLMAPLS"/>
    <dbReference type="OrthoDB" id="273181at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033260">
        <property type="term" value="P:nuclear DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053239">
        <property type="entry name" value="Dual_spec_PTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47550">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE PPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47550:SF1">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE PPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="3">
        <location>
            <begin position="492"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="4">
        <location>
            <begin position="632"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW35571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="720" mass="84864" checksum="8F3A09806A3E54F6" modified="2011-11-16" version="1">MTLSEDLEHLKLNENDTFEFNKHRSASLSSNVSIPSREQSWFTRFNFKLRQKGKNVYVIERISDLKLLFEFYYKHRNRFNFNYSESLFPYLHGLNNSNQCSYFLDEVQLKDIHHQLSNLQLNNVMFLRSDNKKTNIPNLLNTVDIKELLTKEGSYCSLDYDHTSPENEGELNCRNFNKQIKLMAPLSHFVLYNYNNTCNDETMRILRQIIPSDRFIYSIEIDYWWMEIDSSYFDNEYDELKMYNNSMTNEISFSEEKYVSHDEIYDCKFHKYEQNLIWRLNSRKWILNDRVCVGNILDFNNLRLETREFKLIINCHQDAKLPTLELLESIWDNFSLENSQGSVFYLDFPASGSFDPLRITNDELNIILNVLKLIEKISRQNKIFVFSYDGFTGSSLLTICITMLLARKTVEEAIIYLVQSPAKIYFFKGDMSYLSRFERFVDYLNKHLISEFPNIIPPDSLNFSAINRYYHMHPIIKPQPYDWFKLNQDNNFPSRVSPGVYLGSLNHANSRTILRSLKISHMVSIGECPAWWADLDQYITFDFENVSTSKHVLTPIFTFNENCQVYDVDLSQYSIPPRIVPGSVKSLVYIHNFNDDGRDSILPLLLDAPTYIQEKILLGSNKNTFQANEITLVHCKIGASRSASLVLASLMKQHRINLVQAYLALRVLRFNIIIQPNLKLFYDLYLYQSYLGVDEQDNDCKIWNWECLCKEVQLLNDNYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE95</accession>
    <name>G3AE95_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58843</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373041.1">
        <property type="nucleotide sequence ID" value="XM_007372979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE95"/>
    <dbReference type="GeneID" id="18872640"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58843"/>
    <dbReference type="HOGENOM" id="CLU_2980575_0_0_1"/>
    <dbReference type="InParanoid" id="G3AE95"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="58" mass="7026" checksum="F0E3A3DF7F891536" modified="2011-11-16" version="1">MPPSNSLDYLNSFRMSEKIVDYTTLHQKMICRMLLANLMSQWKTYHIVYCSVKYYLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEF1</accession>
    <name>G3AEF1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Flavin-containing monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_131420</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FMO family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373151.1">
        <property type="nucleotide sequence ID" value="XM_007373089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEF1"/>
    <dbReference type="FunCoup" id="G3AEF1">
        <property type="interactions" value="644"/>
    </dbReference>
    <dbReference type="GeneID" id="18869572"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131420"/>
    <dbReference type="eggNOG" id="KOG1399">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006909_5_0_1"/>
    <dbReference type="InParanoid" id="G3AEF1"/>
    <dbReference type="OMA" id="WFDLQYD"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00370">
        <property type="entry name" value="FMOXYGENASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="492" mass="54584" checksum="1B1C2D1676178966" modified="2011-11-16" version="1">MTVYKDIHAAISHINSVAVIGAGPGGASTIKALLAERKFSHVQGFEKRSDFGGIWNHTDITDSHEVPIPCEKPIELNPVVVDGKYVYPSPVYDSLDTNVPKDLMSYSGLEFDSHLPIFPDRRDVLKYVCKYSEKVRPYVRFDTKVVSVELLDDICKWQVVSRPVSTETKGGTIGVDDVVELYDAVVIATGNYDLPYIPDRPGMTAWNEQFPGTITHAKWYRNPRQFKDVTGKIIIVGNSASAGDLAYQIATTLKQKVYKSKRSGSLVPAGKSDLIEEVADIEKFDIDTKSVVLVDGTVLADVGAVVFATGYIKSFPFFKANPTHPLVTDGHKVHGTFNHVILYNYPNLAIIGLPRFVLPTRTSETQSCWLASIWAQKIALPSVQHMQQWEQDRVDLKGNGSKFHDMINLEDVRYCISLTEQVIKAGGNLIPHAWDKQSISVRGMIKSIKEAYIEYKGKTGKCAASYSELIDAGLLDSVTLPDEKLQELGFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEU2</accession>
    <name>G3AEU2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">rRNA 2'-O-methyltransferase fibrillarin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Histone-glutamine methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_131912</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-glutaminyl-[histone H2A] + S-adenosyl-L-methionine = N(5)-methyl-L-glutaminyl-[histone H2A] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50904"/>
            <dbReference type="Rhea" id="RHEA-COMP:12837"/>
            <dbReference type="Rhea" id="RHEA-COMP:12839"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. Fibrillarin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373184.1">
        <property type="nucleotide sequence ID" value="XM_007373122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEU2"/>
    <dbReference type="FunCoup" id="G3AEU2">
        <property type="interactions" value="1104"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEU2"/>
    <dbReference type="GeneID" id="18869619"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131912"/>
    <dbReference type="eggNOG" id="KOG1596">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059055_1_0_1"/>
    <dbReference type="InParanoid" id="G3AEU2"/>
    <dbReference type="OMA" id="WNPNKSK"/>
    <dbReference type="OrthoDB" id="1859733at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990259">
        <property type="term" value="F:histone H2AQ104 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000452">
        <property type="term" value="P:snoRNA guided rRNA 2'-O-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000056">
        <property type="entry name" value="Fibrillarin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000001">
        <property type="entry name" value="Fibrillarin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00351">
        <property type="entry name" value="RNA_methyltransf_FlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000692">
        <property type="entry name" value="Fibrillarin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020813">
        <property type="entry name" value="Fibrillarin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003276">
        <property type="entry name" value="PRK04266.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335:SF17">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335">
        <property type="entry name" value="RRNA 2-O-METHYLTRANSFERASE FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01269">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006540">
        <property type="entry name" value="Nop17p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00052">
        <property type="entry name" value="FIBRILLARIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01206">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00566">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="7"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW35772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="322" mass="33700" checksum="86285962BAA4F10A" modified="2011-11-16" version="1">MSFAPRGRGGARGGSRGGFGGGARGGRGGGRGGFGGGRGGFGGGRGGARGGARGGARGGARGGARGGRGGARGGARGGAKVVIEPHRHAGVFIARGKEDLLVTRNMAPGESVYGEKRITVEEPAKEEGAAPTKIEYRVWNPFRSKLAAGIMGGIDELGIAPGKKVLYLGAASGTSVSHVSDVVGPEGLVYAVEFSHRPGRELIGMAKKRPNVIPIIDDARHPQKYRMLLGMVDCVFADVAQPDQARIIALNSHLFLKDGGVVVISIKANCIDSTVDAETVFAREVQKLREERIKPLEQLTLEPYERDHCIVVGRYMRSGLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEZ5</accession>
    <name>G3AEZ5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">threonine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Threonyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_57871</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="3"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372211.1">
        <property type="nucleotide sequence ID" value="XM_007372149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEZ5"/>
    <dbReference type="FunCoup" id="G3AEZ5">
        <property type="interactions" value="77"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEZ5"/>
    <dbReference type="GeneID" id="18872231"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57871"/>
    <dbReference type="eggNOG" id="KOG1637">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008554_2_1_1"/>
    <dbReference type="InParanoid" id="G3AEZ5"/>
    <dbReference type="OMA" id="SCKGHPL"/>
    <dbReference type="OrthoDB" id="5423599at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070159">
        <property type="term" value="P:mitochondrial threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000039">
        <property type="entry name" value="Threonyl-tRNA synthetase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF50">
        <property type="entry name" value="THREONINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="14">
        <location>
            <begin position="60"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW34799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="473" mass="54567" checksum="065706B7DDE9766F" modified="2011-11-16" version="1">MYVTRAIASELRIARTIPIRTYSSKPKDNNKPKEAISSTQSNHALSQQQLLYTTDPTSPGSIFFLPHGTRIFNKLIQFMKNQQTKYGFQEVVTPLIYKTKLWKQSGHWDNYKEDMFKVLGNDQSRDDELAVEPCGSHHVHEEEFHEYGLKPMNCPGHCMIFAKFERSYNELPIRYSDFSSLHRNEASGALSGLTRVRRFHQDDGHIFCELSQIDQEIKNTINLIKDTYGVFGIGVNDIEFYLSTRPEDKFMGDIETWNVAEQQLKNVLNSTTDKWEIREGDGAFYGPKIDVLLTDAFKKKHQVGTIQLDFQLPSRFELKYISQDGTRTNRPILVHRAVFGSLERFFAILLDHYQGNWPFWINPRQAVIIPVSSSHHEYAEALQKQLSGDILNSEDIAPITGYHFYVDVDKRDATVGLRIKEAIQKGYSYIIMVGDKDIANGTYAIRTRTDRKITSMTGDAIYQMFISLERQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF12</accession>
    <name>G3AF12_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Pump-driving ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_57901</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. Component of the Golgi to ER traffic (GET) complex, which is composed of GET1, GET2 and GET3. Within the complex, GET1 and GET2 form a heterotetramer which is stabilized by phosphatidylinositol binding and which binds to the GET3 homodimer. Interacts with the chloride channel protein GEF1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Golgi apparatus</location>
        </subcellularLocation>
        <text evidence="11">GET1 and GET2 are required for targeting GET3 to the endoplasmic reticulum.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the arsA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372228.1">
        <property type="nucleotide sequence ID" value="XM_007372166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF12"/>
    <dbReference type="FunCoup" id="G3AF12">
        <property type="interactions" value="1007"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF12"/>
    <dbReference type="GeneID" id="18872243"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57901"/>
    <dbReference type="eggNOG" id="KOG2825">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040761_0_0_1"/>
    <dbReference type="InParanoid" id="G3AF12"/>
    <dbReference type="OMA" id="MDAPYEF"/>
    <dbReference type="OrthoDB" id="1770at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006620">
        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02035">
        <property type="entry name" value="ArsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001359">
        <property type="entry name" value="ATPase GET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03112">
        <property type="entry name" value="Asna1_Get3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="ArsA/GET3_ATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027542">
        <property type="entry name" value="ATPase_ArsA/GET3_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00345">
        <property type="entry name" value="GET3_arsA_TRC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="ARSENICAL PUMP-DRIVING ATPASE ARSENITE-TRANSLOCATING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803:SF3">
        <property type="entry name" value="ATPASE GET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <keyword evidence="8 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ArsA/GET3 Anion-transporting ATPase-like" evidence="12">
        <location>
            <begin position="19"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="26"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="348" mass="39371" checksum="58766C0AB00CD187" modified="2011-11-16" version="1">MDFELEPTLETLITQDTLKWIFVGGKGGVGKTTTSSSIAVQLALNHPNDQFLLISTDPAHNLSDAFCQKFGKDARKVEGLPNLSCMEIDPEAAMSDLQQQAQQYNNDPNDPLKSMMNDMTGSIPGIDEALSFMEVLKHIKNQKVNEDDSKDKISYRTIIFDTAPTGHTLRFLQLPSTLQKLLGKFQQLSGKLGPMMSMLGGGANQQDMFAKLNEVQKNVTEVNEQFTNPDLTTFVCVCISEFLSLYETERMIQELMSYQMDVNSIVVNQLLFADDDESPCSRCVSRWKMQKKYLDQMAELYEDYHLVKMPLLGTEIRGVENLTKFSKFLLKPYDPKVDRGIITEMKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF18</accession>
    <name>G3AF18_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_53224</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal/bacterial/fungal opsin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372234.1">
        <property type="nucleotide sequence ID" value="XM_007372172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF18"/>
    <dbReference type="FunCoup" id="G3AF18">
        <property type="interactions" value="60"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF18"/>
    <dbReference type="GeneID" id="18871769"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53224"/>
    <dbReference type="eggNOG" id="ENOG502QZWT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054785_1_1_1"/>
    <dbReference type="InParanoid" id="G3AF18"/>
    <dbReference type="OMA" id="FEYWIIS"/>
    <dbReference type="OrthoDB" id="536545at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15239">
        <property type="entry name" value="7tm_YRO2_fungal-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000160">
        <property type="entry name" value="Related to Opsin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001425">
        <property type="entry name" value="Arc/bac/fun_rhodopsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043476">
        <property type="entry name" value="Yro2-like_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28286">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28286:SF1">
        <property type="entry name" value="30 KDA HEAT SHOCK PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01036">
        <property type="entry name" value="Bac_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01021">
        <property type="entry name" value="Bac_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="295"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="295"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="311"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="326" mass="36146" checksum="BA2952F8DCDFEDDF" modified="2011-11-16" version="1">MLELVKRNTALEVNPTSSFIDIHLTDHGSSWLWAAFAVFGVLTVVHGFLYTLTSVRRSGLKKALLTIPLFTNAIFTYTYFTYASNLGYTGIPTQFNHVTTDEGLDVRQIFYVKFIGWFLAWPFVLSIFEVITHTHLTEEGTDLISKFISLFSGLFTKVVAVEVFVLGLLIGALIESSYKWGYFTFAVFAQLFAMFLIGRDLFHSFGAASRSGLGNMFVLFAFVVWILYPICWGLSEGGNVIQPDSEAAFYGILDVITFGFLPTILTWLAVKNVDEGFFGKIWPYHLKDSVGDNTVDPEKSIGDTTPRHSGDTAVAPAGVPATATDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFL3</accession>
    <name>G3AFL3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GPI inositol-deacylase</fullName>
            <ecNumber evidence="10">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_48058</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in inositol deacylation of GPI-anchored proteins which plays important roles in the quality control and ER-associated degradation of GPI-anchored proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the GPI inositol-deacylase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="10"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372414.1">
        <property type="nucleotide sequence ID" value="XM_007372352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFL3"/>
    <dbReference type="FunCoup" id="G3AFL3">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFL3"/>
    <dbReference type="GeneID" id="18871173"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48058"/>
    <dbReference type="eggNOG" id="KOG3724">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006103_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFL3"/>
    <dbReference type="OMA" id="WVRNLAV"/>
    <dbReference type="OrthoDB" id="348976at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050185">
        <property type="term" value="F:phosphatidylinositol deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006505">
        <property type="term" value="P:GPI anchor metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034368">
        <property type="term" value="P:protein-lipid complex remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016050">
        <property type="term" value="P:vesicle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012908">
        <property type="entry name" value="PGAP1-ab_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039529">
        <property type="entry name" value="PGAP1/BST1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056824">
        <property type="entry name" value="PGAP1_TMD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15495:SF7">
        <property type="entry name" value="GPI INOSITOL-DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15495">
        <property type="entry name" value="NEGATIVE REGULATOR OF VESICLE FORMATION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07819">
        <property type="entry name" value="PGAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25141">
        <property type="entry name" value="PGAP1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25140">
        <property type="entry name" value="PGAP1_TMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="599"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="643"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="701"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="798"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="830"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="862"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="884"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="domain" description="GPI inositol-deacylase PGAP1-like alpha/beta" evidence="11">
        <location>
            <begin position="42"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="GPI inositol-deacylase transmembrane" evidence="12">
        <location>
            <begin position="604"/>
            <end position="899"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW35002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="915" mass="103847" checksum="E22DDE56463903BF" modified="2011-11-16" version="1">MGPSYARIKGFDESYTKFASKYSLYLYREQHKDKIPNEGDFLDGVPVLFIPGNAGSYRQVRAIAARVSELYYDEHDKYKKINPNFKNLDVFAGDFNEDFTAFHGRTILDQAEYLNEAIKFILDLYSSNPSPPKSVIIVGHSMGGIVARVMITLPNYVDNSINTILTLSSPHAAAPLTFDGDILKIYSAIDRFWYEGYNPKSTSEYGEIAHERVKDISLISITGGGLDSTLPADYTSLGFLIPPNNGFTVFTTGTPNVWTPIDHLAIVWCSQLRQFIAESLLEVIDSNAPGKTYPLEKRMRIFRKHFLSGFENYQSQDYESYTRSNLIEVDLKGKTTNKVKQLNMGADYNQYNMFELEKGQHFSLLASVPLGKYQDKANTVVLLCTKGNTLSCVDVSPDMVTIPRSTKDTNSLEDSTFGGSANPFYGLEFNSTILDNYDTILVIQNGELGDDFIVAELNQESSINYKLNASGLSMLGGASLTIPKRALSINIQIPGAWNSILVYKLRLSRLVDGKFTSFMRQWKDEPFESKWYINLTKEKSIGLSMHGIAPFVPFKVKSESYSHAINIQLWSDVTGRDAPLTVNISIDLVSSLRLLVLRYRISIVSMIVAIISMVILFQFRSYGSGKTFPSFINVIAMIHRRGWWILLTVLIMIQPIVTIPFVQHVLNFIDPVVIQDFNEINLSLHPDFKLNSFYLGLDEGFTLVGIILYLIANFLIIITYLVLRILGALIHYVVDIIPVRASRGSSKYRRVIATLLLIGSISTYLPYQIGYAMCCVIQAMNVVKNWKQRQDLNYQLSILLLMLWILPINIPIVIVYIHNLAVNWKTPFSSHHNLLSIVPIVLFVQTNSLLKLPRVKPRYVKVLSWYLAYVIMYAVLYGGRHTYWLHHLMNLLCCVLLLIFYDHDEDDEQQELQCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFN5</accession>
    <name>G3AFN5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Elongin-C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_58156</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SKP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372436.1">
        <property type="nucleotide sequence ID" value="XM_007372374.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFN5"/>
    <dbReference type="FunCoup" id="G3AFN5">
        <property type="interactions" value="672"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFN5"/>
    <dbReference type="GeneID" id="18872346"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58156"/>
    <dbReference type="eggNOG" id="KOG3473">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130038_1_1_1"/>
    <dbReference type="InParanoid" id="G3AFN5"/>
    <dbReference type="OMA" id="ELMMAPN"/>
    <dbReference type="OrthoDB" id="249087at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070449">
        <property type="term" value="C:elongin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000113">
        <property type="term" value="C:nucleotide-excision repair factor 4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070911">
        <property type="term" value="P:global genome nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009411">
        <property type="term" value="P:response to UV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000035">
        <property type="entry name" value="Elongin C transcription elongation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039948">
        <property type="entry name" value="ELC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20648">
        <property type="entry name" value="ELONGIN-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW35024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="98" mass="11163" checksum="C7FBDAA83E4CB720" modified="2011-11-16" version="1">MSSNKEYVTLVSSNGFKFVVLKEVAQISSVLQNSQGFEEGKTGRIELDMEGDILECIVDYLHYNYKYKNLEDIGDIPQFNIPTHLALELLVKADYLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFR1</accession>
    <name>G3AFR1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptide hydrolase</fullName>
            <ecNumber evidence="1">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_48101</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M28 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="1"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372462.1">
        <property type="nucleotide sequence ID" value="XM_007372400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFR1"/>
    <dbReference type="STRING" id="619300.G3AFR1"/>
    <dbReference type="GeneID" id="18871177"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48101"/>
    <dbReference type="eggNOG" id="KOG2195">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005688_2_0_1"/>
    <dbReference type="InParanoid" id="G3AFR1"/>
    <dbReference type="OMA" id="YYSYDWL"/>
    <dbReference type="OrthoDB" id="5841748at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF46">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="4">
        <location>
            <begin position="281"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="5">
        <location>
            <begin position="443"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="80"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="858" mass="97927" checksum="72CD5F8629157B36" modified="2011-11-16" version="1">MTYATEDTGSSHNQDSEFTPLLTSSKGSNSSSHGGLDEGLDLSSSQETSGEVNMKPHEYLVSPPPTGSEIYSRQQQQQQEQDEEDDDDDDDDNVSRILKIAKRPSFIWICTLGVVAFIIFQLTFLPRTSLGRDYRRWHGIHLTKSDVKRLFLQMAGIGNSHNSLTTEEYIDSWLTNFTSINSKSTVNMIADDNLELVSFVETNFKQFGFKTESFSYDAPHSLTRPVSSTLRLLDKNGQVAYSPVVGEPNFKTPAFYAFGTNASIVANFVFINEGTIHDYNLLEKHSIDVHNKIVIIKSNLCNKNITIAEKVAIAKQRGASGVINYYDFSASNNVENESSLAHAISRASIFQQFRLPSIPVSKKIIKPILDTLSADPQPDFSHWDYPPVGSTPMKVELNTTFAAAPLHPAKLTTVVASLKGIMNDADVIIGARRDSYTCNNPLSGHAILLEIMRNYQRLVAKGWKPLRNIKFVSWDGSNTDLIGSHKFTDDVNVYNPKRSIVAYINIDGDAVMGSKLAVDANPMLHSLLRNTAKFIPIPKEATSYKTLKNDDDDEDDEGYTTLHRYWMKQDNATINSVMGLPISQTESIIFQQHLGTPSINIKFENDAKRDSSVYVPNSNFYSKDWLIKREIDDDLLLHGSMIRFIGLLAISLSEHEVVSYKTHSYFKSIEGYFNELLEDKRNTLGFWHSEPVSNYVIAKSSIYQDLGIDNVKFGDLIRQFKHLMNETVYQSGIFDEWNNHVEDMLMQDYPWYLYYKKLQHFAQFKVTNYKLSHLERDLKLQDKDYQYLNKDKSYYDSVIYGTKKFDPAEVTQEYFNDKLKTSTFTHLYSAIEVRDFEMTVKWLVLIYEKFKNLRYKMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFZ0</accession>
    <name>G3AFZ0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_53469</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372541.1">
        <property type="nucleotide sequence ID" value="XM_007372479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFZ0"/>
    <dbReference type="FunCoup" id="G3AFZ0">
        <property type="interactions" value="286"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFZ0"/>
    <dbReference type="GeneID" id="18871786"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53469"/>
    <dbReference type="eggNOG" id="KOG0588">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005306_1_0_1"/>
    <dbReference type="InParanoid" id="G3AFZ0"/>
    <dbReference type="OMA" id="YNKRRDR"/>
    <dbReference type="OrthoDB" id="504170at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005940">
        <property type="term" value="C:septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060258">
        <property type="term" value="P:negative regulation of filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001558">
        <property type="term" value="P:regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12194">
        <property type="entry name" value="Kcc4p_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14081">
        <property type="entry name" value="STKc_BRSK1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000394">
        <property type="entry name" value="Serine/threonine-protein kinase HSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.220">
        <property type="entry name" value="Fungal kinase associated-1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031850">
        <property type="entry name" value="Fungal_KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043024">
        <property type="entry name" value="KA1_sf_fungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16797">
        <property type="entry name" value="Fungal_KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="26"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="370"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="523"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="793"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="938"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1052"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="640"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="379"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="410"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="463"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="523"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="542"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="552"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="793"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="803"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="939"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="959"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="977"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1061"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1098"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW35129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1261" mass="142840" checksum="87CD78388D7B9E14" modified="2011-11-16" version="1">MPHSRQASISSSMDAGHSHPQKIGPWKLGKTLGRGATGRVLLATHQVTGQKAAVKVVSKSELQDAEDEPQDLDQKKEGLPYGIEREIIIMKLLNHPNVLRLYDVWETSKALYLVMEYVEGGELFDLLVERGPLQEREAIKYFRQIILGTAYCHALGICHRDLKPENLLLDDQLNVKLADFGMAALESNGKLLETSCGSPHYAAPEIVSGLKYHGAASDIWSCGVILFALLTGRLPFDDENIRNLLLKVQAGSFEMPDDLSREAQDLISKMLNADPAKRIKIDVILKHPLLLKYPIPNEDLISERSLPHPQTAYKSLGSVKNIDKQILSNLSILWHNRSQQDIIESLLKNGPNPEKTFYALLMRYRHNTNQEDFGVGNGTVPNRSPPKKSTSYNKLPATSTTPKKNKRSSKINVSRPTSFQYNRNSYVKTTNPTTSSPHGSPYKTSPAKRYSPRKSPRYSPLKSPRHSPLKSPRHSPRKTGDKRLSGLGSIPRNLYNEIMAAQSNQSIPPSLPSKGSYYEKHELTNVPENKRLDESAKISPNKRASIFSSSKSSKRLSKRKSIRASMTTGLKRNSITMKLLSTYAKLSTDNEWEYVDKQAKRTSATFATLCDKIFNHEDYDPEEEKLADPEEKQAMEYERLMEIERKKHEAEIKARKELEKRKRRQNRKSLLTGNQKLTISIKDDNEANNSEQDVFARELITQPKRQSKPPAGVRASSEGILESEELTREELECLKKRSVTQPVPARRRDPVLTRRPVSRLDPLWQAYENQQLDEAKDALEQEWRDGLLNKHPKRESTVKRESVTTTKRMSVATNRHSTVSKRINRESMVSILDNDDEEDDGFDFTINKPQQSIPPIEGKRLSKDVGAKTLIGNVKIPNVTRKSRNFTNSNKRLSVLSLYSTKESYHDLNSFINDEDDVQQQLPKLRTSLADRLNQAGLVEEDEEEEAEQYEDNGSVLDLDDHANKRYSYHDKRLSTTRRYNPPTGTRPTSKIPELPKTEYDDTFATQSDDIYKKRKSMVSVESEEPTDDVFDKIKLPEGKSTKSSIDALANEVPSGKKSMLSPTLNDEPIQSQPYPKVRDKANTSAPLAPLGPPGRSDGLKKPLEDKTNIYEKRKPSIFRKLSWGTKKALDEPEEKPKSSFLRWFSSSSTSSQIQIKKIQTLLPQHEMSIALFSLLNSWTNFGLKKLKNDQIGYNITGSISKKSASSCKFRIKINPRDFNQKSELVCVRVKGSQETADLLFREIEQVLRSEDALDVEASTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG65</accession>
    <name>G3AG65_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GPI-anchor transamidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_58405</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C13 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372616.1">
        <property type="nucleotide sequence ID" value="XM_007372554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG65"/>
    <dbReference type="FunCoup" id="G3AG65">
        <property type="interactions" value="674"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG65"/>
    <dbReference type="MEROPS" id="C13.005"/>
    <dbReference type="GeneID" id="18872437"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58405"/>
    <dbReference type="eggNOG" id="KOG1349">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044656_2_1_1"/>
    <dbReference type="InParanoid" id="G3AG65"/>
    <dbReference type="OMA" id="VMESQFP"/>
    <dbReference type="OrthoDB" id="192611at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003923">
        <property type="term" value="F:GPI-anchor transamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000003">
        <property type="entry name" value="GPI-anchor transamidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028361">
        <property type="entry name" value="GPI_transamidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001096">
        <property type="entry name" value="Peptidase_C13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48067">
        <property type="entry name" value="GPI-ANCHOR TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48067:SF1">
        <property type="entry name" value="GPI-ANCHOR TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01650">
        <property type="entry name" value="Peptidase_C13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500138">
        <property type="entry name" value="GPI8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019663">
        <property type="entry name" value="Legumain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00776">
        <property type="entry name" value="HEMOGLOBNASE"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5028258319" description="GPI-anchor transamidase" evidence="6">
        <location>
            <begin position="24"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR019663-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="381" mass="43270" checksum="83AC5B10DC8D34B3" modified="2011-11-16" version="1">MKLLFQLLLTFFIVCGIAESTESADPISEGNYEVGKHTNNWAVLVSTSRFWFNYRHMANVLSLYRTVKRMGIPDSQIILMLSDDVACNSRNAFPGTVFNNMDQAIDLYGDSIEVDYRGYEVTVENFIRLLTDRWDDEQPRSKRLLTDENSNIFIYLTGHGGNEFLKFQDAEEIGAYDIADAFAQMHEKKRYNEIFFMIDTCQANTMYEKIYSPNILAVGSSAFDESSYSHHSDMDIGVAVIDRFTYYTLDFLEKIPKDSKVTMDKLFEEYTFENVHSHPGIRTDLFQRNISEVLLSDFFGNVQKVIVDNVESDLLSIANNKSDSQSSTIKRNIKRGPIEFGHDSEEGATFLPGISPSLQAKLLGGATVVVMLGLWLVSPQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGK4</accession>
    <name>G3AGK4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Prokaryotic-type class I peptide chain release factors domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58571</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372755.1">
        <property type="nucleotide sequence ID" value="XM_007372693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGK4"/>
    <dbReference type="FunCoup" id="G3AGK4">
        <property type="interactions" value="250"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGK4"/>
    <dbReference type="GeneID" id="18872507"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58571"/>
    <dbReference type="eggNOG" id="KOG3429">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089470_0_2_1"/>
    <dbReference type="InParanoid" id="G3AGK4"/>
    <dbReference type="OMA" id="WYNSFDA"/>
    <dbReference type="OrthoDB" id="270639at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016150">
        <property type="term" value="F:translation release factor activity, codon nonspecific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070126">
        <property type="term" value="P:mitochondrial translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052104">
        <property type="entry name" value="Mito_Release_Factor_mL62"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11075:SF54">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11075">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110916">
        <property type="entry name" value="Peptidyl-tRNA hydrolase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prokaryotic-type class I peptide chain release factors" evidence="1">
        <location>
            <begin position="51"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="196" mass="22542" checksum="342C950F3F7B5CEC" modified="2011-11-16" version="1">MMFYKLPLYSHCTSILRLSVRVSPIVRFNSSITEFTTKEIDASKKWVEKFTPIQIPTDVFTISYSRSSGPGGQKVNKTSSKATVSLEPGQWLTPATSFWIPQPIQHQLKENKIRYETKGGGLLIQSDVFRSRDANTSECFKKLLEEIKSKVYFPGEVSEQAKQKWEKLEQAAKERRKVEKKILSEKKKSRSKSFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGT1</accession>
    <name>G3AGT1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="10">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_69665</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="10"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372826.1">
        <property type="nucleotide sequence ID" value="XM_007372764.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AGT1">
        <property type="interactions" value="73"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGT1"/>
    <dbReference type="GeneID" id="18875282"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_69665"/>
    <dbReference type="eggNOG" id="KOG1140">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000684_1_0_1"/>
    <dbReference type="InParanoid" id="G3AGT1"/>
    <dbReference type="OMA" id="GEASYMC"/>
    <dbReference type="OrthoDB" id="26387at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19672">
        <property type="entry name" value="UBR-box_UBR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.30:FF:000002">
        <property type="entry name" value="Putative e3 ubiquitin-protein ligase ubr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044046">
        <property type="entry name" value="E3_ligase_UBR-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055194">
        <property type="entry name" value="UBR1-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF24">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18995">
        <property type="entry name" value="PRT6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22960">
        <property type="entry name" value="WHD_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="11">
        <location>
            <begin position="91"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="9">
        <location>
            <begin position="91"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW35414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1805" mass="207558" checksum="0EEC238464734399" modified="2011-11-16" version="1">MDDNISTLKRFLLHLPSESSFKFTNDVRTDIRRALYRAISNQGQFLDWFFPGSSQDNIEECQWLYTEYLKSRQLKDDKNTNANALASHPNSPCARIFRKGEPIYRCLTCGFDETCAFCHNCFQPDFHANHKVHITICLRENGGVCDCGDPEAWIEEFTCPYASSSDNSNCLANEPPSELAGAVLGTFETLLDFIIDVMSQCYLQFEDIESITKDKIELNTINSTFDPRKYASADDNFQDVNNDQYYIQVYNDQIRHYRDAVQRIHLASQKVKPFAEMITDQIHKQGRAKVISSKNLQLLLERQKILSATGLSSCIRSHRDIFREQVCDEIVKWLGDLSESQVFKINADIKDLFCRAFCDKWESGLLIPSFDPAYSYKVGTLDGSLRIPKIDSKAPTSSVYPQWAFTSSKWDLPDEINNQCSYNKDLGDFTAAAHHGSRLQYLFYLDIRFSKSTRVLLHDIYSTSLITNLHYKNIISAQYVDIYPMVADQFLTLDREPELSVMCTLSTQLFTCPTNSTSIIHHGDIARVLASIYGFLTTEKVQSPETLDLTTEISIKSLKNRRWGQIFFDISYILSRSRDPKSILNSNIIPMACDLLALLQGKPGLKRETENHVEYESPDYTAFFHGIPVIYQFAEFIARSLNGVENERKEWGRRAIKYVMNYLQKMHQERHKGEVSFLHPLHSFLSWLIELSQIEPAALAKILQEYQIDNIFTYPITTLVLMSQIKTGYWVRNGFTVRAQLQLYRTTGLRESGYLRDLYLTQIYISSTDPEPTTMNILSQWQLNDWKTYDNNTLPYMIEECLTFFIHVLTEDLNLSQLSEEALLQKRIEREIIHTLCFGGMNYAKLCSHIPDHIVAEKRFDIILTQLTVFKPPKGENDVGVYYLKDEYLDCVNPYYFNYTTNIKDDAIKFIKERVHQKTGKSIGEVVVPAYASKVKDYKIGNFSVSLCFARFLKKVLEFVDENRESLLETCLHLIHILALENNVDEGKFYERFVEVEDHGSIASLIYELLSKEEFSCQHAKIRAILKVFADRHDLPHELNGQIPQFDTSAIDSDYVDNSEDEFEKKKRIAKDRQARLLAKFKKQQSSFLKNNQMDTSDAEMEDEELQHGWKFPEPHCLLCQNAAENAGPFGLITYVAKSSEFRNVPFDDEYWFLRSFSDGVDLDADEENPTTSSHNQAKTARWEEYMRAVKEKFVIGPGFTSHDDVDSKLVSSSCGHGMHFQCYINFLNTNRSRMSQITRNTPENIDHKEFVCPLCKAINNMFIPILWTTNQRSLSKFLTPLPDSSRNPFHNLSIERVHDKEWYDKFTKLSDADIDAISILNPISREIICSGPTAQFTPQQHQFRQLLSNMFQILSFLTFPQVFKADSIFVLVNTIKSIEICHRGISSDGRLIISQLSNNCLINLRTFNEFRNTSVLMKIKNWIHTPNPRGDAYAKMLANIFALSKGNGAINSSIVEADMFECLVNIIPLPSFGLSFHAILEVTFVAHLIQTLNILAKELSSKKNWKYDMWDIPIVADITTTIAESAHLCFVRLATNENIKTLSEAEKVGRVIYSMTVKAATPFLRRAAIYAYVQCADIDDIDFSLYPETEIEANRLCSFMNIKPISEYLEIFASNTASFESRMFDDFVNYVHLSSKSLTISPLARKLEYPGLVKLVELPDRLDRFFTDYYYSDAYAQPHLRIEDPAICLFCASVVDAQKQARGAREGQCTTHYLKECPHDVGVFLLPKDRTLLLLHKNGGSFYKAPYLDEHGEIADDSRRGKTMYLMKTRYDAFIRSVWLSHNVPNYIVRNLESVLDPGGWETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHB5</accession>
    <name>G3AHB5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GABA-specific permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_148468</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373663.1">
        <property type="nucleotide sequence ID" value="XM_007373601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHB5"/>
    <dbReference type="FunCoup" id="G3AHB5">
        <property type="interactions" value="201"/>
    </dbReference>
    <dbReference type="GeneID" id="18870785"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_148468"/>
    <dbReference type="eggNOG" id="KOG1289">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004495_0_3_1"/>
    <dbReference type="InParanoid" id="G3AHB5"/>
    <dbReference type="OMA" id="RMIWGVV"/>
    <dbReference type="OrthoDB" id="4476201at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF6">
        <property type="entry name" value="GABA-SPECIFIC PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="284"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="296"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="352"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="404"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="431"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="507"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34079.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="558" mass="61331" checksum="C581F75C6A15DCEA" modified="2011-11-16" version="1">MDAIRSRNSEHLNVITSIRSKTEDHLHHIASIRSEIREIDPSQAHNDVELLAQIGYKQELRRHYSTLQVFGIAYSIMGLLPSISSTIATGLESGPAGLVWGWFVASIFILSTGTSMSFLGSAIPTSGGLYYYTNYYAPHWIRVPLSFLIGCSNTLGLIGGLCSISYGFAVEVLSAVFIQTDGDFEITNAKCYGLFAACIISNVIISCLTTKYAAHWQTISIIVNTFLIILFMIAVPAGKKHDFNSASYIFTNFENARTTYSTAWSFFMGFMPAIWTIGAFDSAIHCSEEAKNAQRAIPVGIVGSISACWILGWLICIVCAACIKDGDTARVLSSETGNPMAQIIYDALGKKWAVAFMAMIAVGQYMMSVSILIALSRQIWSFARDNGLPIVYNFIKVINPKIQVPVRATIFAGCLSLVLGLLVLINGTAGANALFSLAVACNLLAWGTPVFLVLLPLGRKRFVSGPFYFGKIISRIINCVTSCWVVFVIVLAMFPDSRDVDKDSMNYTVVINCGMWILSLIYFYVWGYRTYTGPVSNLDDEYSEGSIQHLDEVLKEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHK0</accession>
    <name>G3AHK0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phosphoribosylformylglycinamidine synthase</fullName>
            <ecNumber evidence="4">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Formylglycinamide ribonucleotide amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Formylglycinamide ribotide amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">SPAPADRAFT_65329</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the FGAMS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="4"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373748.1">
        <property type="nucleotide sequence ID" value="XM_007373686.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AHK0">
        <property type="interactions" value="977"/>
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    <dbReference type="STRING" id="619300.G3AHK0"/>
    <dbReference type="GeneID" id="18875035"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65329"/>
    <dbReference type="eggNOG" id="KOG1907">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001031_0_2_1"/>
    <dbReference type="InParanoid" id="G3AHK0"/>
    <dbReference type="OMA" id="LSANWMW"/>
    <dbReference type="OrthoDB" id="6666987at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01740">
        <property type="entry name" value="GATase1_FGAR_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02203">
        <property type="entry name" value="PurL_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02204">
        <property type="entry name" value="PurL_repeat2"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.750">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase, linker domain"/>
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    </dbReference>
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        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00419">
        <property type="entry name" value="PurL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040707">
        <property type="entry name" value="FGAR-AT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055181">
        <property type="entry name" value="FGAR-AT_PurM_N-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010073">
        <property type="entry name" value="PurL_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036604">
        <property type="entry name" value="PurS-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01735">
        <property type="entry name" value="FGAM_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003672">
        <property type="entry name" value="PRK05297.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10099">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10099:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18076">
        <property type="entry name" value="FGAR-AT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22689">
        <property type="entry name" value="FGAR-AT_PurM_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13507">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01211">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109736">
        <property type="entry name" value="FGAM synthase PurL, linker domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82697">
        <property type="entry name" value="PurS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribosylformylglycinamidine synthase N-terminal" evidence="21">
        <location>
            <begin position="42"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosylformylglycinamidine synthase linker" evidence="20">
        <location>
            <begin position="207"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="19">
        <location>
            <begin position="471"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="domain" description="FGAR-AT PurM N-terminal-like" evidence="22">
        <location>
            <begin position="688"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="19">
        <location>
            <begin position="864"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004920"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00057317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00071729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF18072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF18076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF22689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="EGW34164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1339" mass="146499" checksum="14EE178971D396ED" modified="2011-11-16" version="1">MSNFLILPGPKALSSFRVNNFIQEIEKKVNQTGAIEAVATSYTHYVSLKQQLSDKQLQLLKILLTYDTPTDAENKLSQQLVKYSTISGDEDTSAIQLDDTTYLVQVLPRPGTISPWSSKATDIAQICGLSDQIERIERGLSLIIKTKPGFNLGQFLTAGGEILTSVFDRMTQTLYLGANAPKYNDLFAHHSPKPLVTVDIISNKENLNKANKELGLALDSGEIQYLINAFTEVIGRNPTDVELFMFAQVNSEHCRHKIFNADWTIDNVKKDLSLFKMIRNTHQKNPLYTISAYSDNAAVFEGPEGYVFAPDFNTKQWKSIREKVHTLVKVETHNHPTAVSPFAGAATGSGGEIRDEGAVGRGSKSKAGLAGFSVADLNIPTLPQPWERNVGKPNHIASSLDIMIEAPIGSAAFNNEFGRPAINGYFRTLTTEVENSKGETEIRGFHKPIMLAGGMGAIRPDLALKNVKITPGAKLIVLGGQSMLIGLGGGAASSISSGEGSADLDFASVQRGNPEIQRRAQQVIDACVSLGKDGNPIQSIHDVGAGGLSNALPELVHDNDLGAKFELRSILSLEPGMSPMEIWCNESQERYVLGVAPENLELFAGICERERAPFAVVGEATEEQRLILTDSLLKTTPIDLEMSVLFGKPPKMSRTAITQNLNLKPFSTNDLDVSESISRVLQLPSVGSKNFLITIGDRFITGLVDRDQMVGPWQVPVADVGVTATSLGETVLATGEAMAMGEKPTLALISASSSAKMCVAESLLNIFAADIPSLGHVKLSANWMSAASHDGEGAKLYEAVQAIGLDLCPDLGISIPVGKDSMSMKMKWDDKEVTAPLALTITSFAPVDNTSNTWTPYLQPVEDSVLVLVDLAAKVKKALGGSALAQVYKQVGDAAPTVHSHQILKSFLQTLVVLHKKFDVLAYHDRSEGGLITTAVEMAFASRSGLDLTIQGEDLFTELFNEELGAVFQIRSSEYEQFKEIFATNGISSEYISTIGKPNFQAQTVNVAFNGQQVYESTRGHLQQLWSNTSYHIQKLRDNPETSTQEYQAISDDKDPGLSYQLTFNPSEAKEFSRKPKVAILREQGVNSQQEMAWSFQQAGFDVYDVTMTDILENRVSLDDFVGLAACGGFSYGDVLGAGAGWAKSVLFHEKTRNEFKKFFQDRTDTFAFGACNGCQFFSRIAELIPGTENWPTFERNLSEQYEARFVMVEVEASEKNNSIFLQKMKGSKLPIAVAHGEGRAQFKSSETQARFHSDVINYVDNYGKVTEQYPFNPNGSPNGIAGISNANGRVLAMMPHPERVTRKESNSYYPVEQGKQWGVHGPWIQLFRSAREWVGDNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI06</accession>
    <name>G3AI06_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_149025</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373902.1">
        <property type="nucleotide sequence ID" value="XM_007373840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI06"/>
    <dbReference type="GeneID" id="18870808"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_149025"/>
    <dbReference type="eggNOG" id="ENOG502RQ1I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1026700_0_0_1"/>
    <dbReference type="InParanoid" id="G3AI06"/>
    <dbReference type="OMA" id="FMAADVE"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008402">
        <property type="entry name" value="APC_su15/mnd2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05841">
        <property type="entry name" value="Apc15p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="319" mass="35074" checksum="96CBD6B4CAD90B08" modified="2011-11-16" version="1">MYAPSSFHIDLYSLWNGVSSHPTLIPNESNESKSNAEPQTRSTNNGVTTSDQRKHINNLFAPPLSAYSANYPGACSFQLEYTMRRGTQHNSKMFSITPSTQANIEQLDVIRNYGYNTIRPIGINKTMEEIEFENSGQFTVTDEQSYGAGVNNTGAENSGDQQDQQDNSNNTLLLGSNLNASGLTFQLGDESREVDLDAQILNADDLYSGAEDEDDDVDSEADTGFRTNLVDEGFMASEVEYQDDHSLNSEINSTYVVNSGGSSSLFNSNNASGRTIPTNPTTASAPGINNSYYMQRIHEEGHNENDPDYSELDMVVDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIB7</accession>
    <name>G3AIB7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_147604</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373270.1">
        <property type="nucleotide sequence ID" value="XM_007373208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIB7"/>
    <dbReference type="GeneID" id="18870758"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_147604"/>
    <dbReference type="eggNOG" id="ENOG502QPJP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067134_1_0_1"/>
    <dbReference type="InParanoid" id="G3AIB7"/>
    <dbReference type="OMA" id="DARWCID"/>
    <dbReference type="OrthoDB" id="4092858at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021017">
        <property type="entry name" value="Mediator_Med2_fun"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11214">
        <property type="entry name" value="Med2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="128" mass="15089" checksum="17B802293A323862" modified="2011-11-16" version="1" fragment="single">MSSANLQTKLDASLNDILKTSGYIFEVLNNNKKQSNLLTGPNNQLITPQIIAQLSHQMLKFDDILDETITKFNDARWCIDQMVENKQRQEEMKIREEQERARRLKEEEEQKQRRIKEEQEEQARAKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIG0</accession>
    <name>G3AIG0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_134044</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374014.1">
        <property type="nucleotide sequence ID" value="XM_007373952.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIG0"/>
    <dbReference type="FunCoup" id="G3AIG0">
        <property type="interactions" value="249"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIG0"/>
    <dbReference type="GeneID" id="18869778"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134044"/>
    <dbReference type="eggNOG" id="KOG1989">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011638_4_0_1"/>
    <dbReference type="InParanoid" id="G3AIG0"/>
    <dbReference type="OMA" id="TIGVAMC"/>
    <dbReference type="OrthoDB" id="2018507at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000147">
        <property type="term" value="P:actin cortical patch assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000441">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967:SF57">
        <property type="entry name" value="AUXILIN, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="22"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="494"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="569"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="499"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="591"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="638" mass="71504" checksum="AB0A18C322B18FA1" modified="2011-11-16" version="1">MPTPPPNALTPGTNLEVGIHQISIIKYISEGGFAHVYTCTVDPPFHGSSIACLKRVVVPSKWQLSLLRQEVDAMRRLRGNKHIVSYIDSHAARLPKSDNDSTQKYEVFLLMEYCERKGLIDFMNTRLVNKLTEPEIVDIMYQVTIGVAMCHNLRPPLIHRDIKIENVLIDGKGTFKLCDFGSSVPYQPPPKSPQELQLLRDELQQHTTPQYRAPEMIDLTKGFPIDDKSDIWALGIFLYKLCYYTTPFEHPSQTSLSELEDTILNCGETLRFRDAPGSMFSHRMKNVIKVCLRGDPRRRPNAIQLLGEICAMKGVPIPDVIPYSVREQIKAESAANVKQPPRGPIKPVLAPTNSSKSEIAAKKIEAPNGNQPTDPFMLDKSVMLDKLTRQKITSNPPRPPARPLSAFYSEESTSRVYTHGNKSSPSVHDHVKAELPKQHTFEFENDTNGTLEFLKSRERKADTPFDNVRRAGENVLGTFGTLGSEAIEIVKSTLTGEHTGGKQKESRKSLESVQTQEPLQKISIQKRIQMLFSDSQKTTKSAGGYDRFTGGNVGEDIDAINVSSDSVKIAQSSSKKSLTPPEIPLALSSHRLNKTKKKKPPKPRKPAYLQGSKEDSDEYDEDIEVLEQQFAKRFPSYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3AII5</accession>
    <name>G3AII5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59067</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373284.1">
        <property type="nucleotide sequence ID" value="XM_007373222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AII5"/>
    <dbReference type="GeneID" id="18872752"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59067"/>
    <dbReference type="HOGENOM" id="CLU_2446812_0_0_1"/>
    <dbReference type="InParanoid" id="G3AII5"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="90" mass="9834" checksum="04A9F9FB95678CF7" modified="2011-11-16" version="1" fragment="single">MNQLIRSSTASRLLGKQLAPASILSRYSLARIIIPYATRNFTTYSHLRQTQSTFKPALAPPSQASQLPSPPQLQSLPPITKATLLSQTNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIL1</accession>
    <name>G3AIL1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_59091</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373310.1">
        <property type="nucleotide sequence ID" value="XM_007373248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIL1"/>
    <dbReference type="FunCoup" id="G3AIL1">
        <property type="interactions" value="852"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIL1"/>
    <dbReference type="GeneID" id="18872767"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59091"/>
    <dbReference type="eggNOG" id="KOG1180">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_45_2_1"/>
    <dbReference type="InParanoid" id="G3AIL1"/>
    <dbReference type="OMA" id="WEWLASI"/>
    <dbReference type="OrthoDB" id="1700726at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF83">
        <property type="entry name" value="ACYL-COA SYNTHETASE LONG-CHAIN, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="556" mass="60914" checksum="FB40BBC276AA360D" modified="2011-11-16" version="1">MACAYQNIPIVTAYDTLGERGLTHSLVQTESDAIFTEPQLLSALIEPLKKATHVKTIIHSEPIDEKDTRHGGKLYKNAQKAKAKILEIRPDIKFISYDEILELGKTSKNETHYPKPEDLLCIMYTSGSTGDPKGVVISNTNLLAAIGGLSEIADHKLVQPHDRVIAFLPLAHIFEMMFECISMWWGVPLGYANVKTLTDASCRNCESDLAAFKPTVMVGVAAVWESVRKGILAKVGQASPLQQKIFWSAYKAKSVLSHYHIPGANIFDVVFKKVKQATGGHLRLILNGGSPISVDAQVFLSNLISPMLLGYGLTETCANATVTEPERLEPGVLGTLTGAITAKLIDVPDAGYFAKDNQGEILLQGACITKEYFKNEAETAEAFTEDGWFKTGDIGEWTSNGGLKIIDRRKNLVKTLNGEYIALEKLESVYRSSPLVLNVCVYADQTQVKPVAIVLPAEATLRKHLVSEHVYSQADIDKTEFAALCHDPKVASSFTKSLISTGTHQGLKGIELIQNVVLLDDEWTPQNGFVTSAQKLQRKKILASCKDRVDAAYKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIZ0</accession>
    <name>G3AIZ0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein PBDC1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_59156</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PBDC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373385.1">
        <property type="nucleotide sequence ID" value="XM_007373323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIZ0"/>
    <dbReference type="FunCoup" id="G3AIZ0">
        <property type="interactions" value="640"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIZ0"/>
    <dbReference type="GeneID" id="18872798"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59156"/>
    <dbReference type="eggNOG" id="KOG4093">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103791_1_0_1"/>
    <dbReference type="InParanoid" id="G3AIZ0"/>
    <dbReference type="OMA" id="IQFYAFE"/>
    <dbReference type="OrthoDB" id="10248897at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990593">
        <property type="term" value="F:nascent polypeptide-associated complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061770">
        <property type="term" value="F:translation elongation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051083">
        <property type="term" value="P:'de novo' cotranslational protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3560.10:FF:000001">
        <property type="entry name" value="Protein PBDC1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3560.10">
        <property type="entry name" value="yst0336 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023139">
        <property type="entry name" value="PBDC1-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008476">
        <property type="entry name" value="PBDC1_metazoa/fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021148">
        <property type="entry name" value="Polysacc_synth_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13410">
        <property type="entry name" value="PROTEIN PBDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13410:SF9">
        <property type="entry name" value="PROTEIN PBDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04669">
        <property type="entry name" value="PBDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polysaccharide biosynthesis" evidence="5">
        <location>
            <begin position="16"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00061201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00069779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW33801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="145" mass="16763" checksum="182E18BCE73EED1E" modified="2011-11-16" version="1">MATQFDADNADNLGEIEMQFAVKAVQQAETYWSLLEKIPGSKLKLTKFDDDIFTCLLEAFPEFKEPANVAQISEEDMKSAAGKTKWREFCEQFKDIEDYNFGTLLRLKASEEYTQEGTIFAVRIQFYAIEIARNRYGLNDWACKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ05</accession>
    <name>G3AJ05_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Palmitoyltransferase</fullName>
            <ecNumber evidence="12">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_135163</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family. SWF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="12"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374101.1">
        <property type="nucleotide sequence ID" value="XM_007374039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ05"/>
    <dbReference type="FunCoup" id="G3AJ05">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ05"/>
    <dbReference type="GeneID" id="18869872"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135163"/>
    <dbReference type="eggNOG" id="KOG1312">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042181_2_0_1"/>
    <dbReference type="InParanoid" id="G3AJ05"/>
    <dbReference type="OMA" id="HIYLIWA"/>
    <dbReference type="OrthoDB" id="9909019at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF489">
        <property type="entry name" value="PALMITOYLTRANSFERASE SWF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="257"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="13">
        <location>
            <begin position="148"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="376" mass="43230" checksum="004C7D387C34194E" modified="2011-11-16" version="1">MLFILILGITIISSFLSAIVIFGDSPSFRNTPIHKLRKAIFTTTEQITHVYNYLDRKTNGKLLKWLSWLVPSGYLAVVTFCIYQFLVKTIPMVTTSMIGPYYKTDITFIIILVYSSTILAIFSDPGVVSSKKPVPHNYKFQNNQLIFFNDKTCNTCHIVKPPRSKHCSICGHCYLLYDHHCVWVNNCIGYYNYKWFLLFLVANIIMLGYGGYVCYQALIFQFRHNLGGADNGGANNAISKFWKLITKTTDANKITGIFLILCIIFIIITTLFTGLHLQYVYLGVTTNELDKWSEIEHLIRLRALYRVEPPVNNEEPFVEQVLLSSGEPAYLSLKSEEILINRTNINNFTLTCINSMEHELTNVYDKGFWNNLKERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ50</accession>
    <name>G3AJ50_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Hydroxymethylglutaryl-CoA synthase</fullName>
            <shortName evidence="6">HMG-CoA synthase</shortName>
            <ecNumber evidence="6">2.3.3.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">3-hydroxy-3-methylglutaryl coenzyme A synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_59164</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form HMG-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>acetoacetyl-CoA + acetyl-CoA + H2O = (3S)-3-hydroxy-3-methylglutaryl-CoA + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10188"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.10"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the thiolase-like superfamily. HMG-CoA synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.10" evidence="6"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373391.1">
        <property type="nucleotide sequence ID" value="XM_007373329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ50"/>
    <dbReference type="FunCoup" id="G3AJ50">
        <property type="interactions" value="680"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ50"/>
    <dbReference type="GeneID" id="18872802"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59164"/>
    <dbReference type="eggNOG" id="KOG1393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008065_0_1_1"/>
    <dbReference type="InParanoid" id="G3AJ50"/>
    <dbReference type="OMA" id="DDAYNWI"/>
    <dbReference type="OrthoDB" id="1269963at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004421">
        <property type="term" value="F:hydroxymethylglutaryl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00827">
        <property type="entry name" value="init_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000008">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000590">
        <property type="entry name" value="HMG_CoA_synt_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013746">
        <property type="entry name" value="HMG_CoA_synt_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013528">
        <property type="entry name" value="HMG_CoA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010122">
        <property type="entry name" value="HMG_CoA_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01833">
        <property type="entry name" value="HMG-CoA-S_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323:SF2">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08540">
        <property type="entry name" value="HMG_CoA_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01154">
        <property type="entry name" value="HMG_CoA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01226">
        <property type="entry name" value="HMG_COA_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase N-terminal" evidence="7">
        <location>
            <begin position="4"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase C-terminal" evidence="8">
        <location>
            <begin position="174"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="4">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00073381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="447" mass="49478" checksum="10C2CCD9CAE5CC11" modified="2011-11-16" version="1">MTQQKIGIKAIEVYIPGQAVNQTELEKFDNIPAGKYTIGLGQTNMAFVNDREDIYSISLTVLSKLIKNYNIDTDKIGRLEVGTETLLDKSKSVKSVLMQLFPGNNDIEGIDTINACYGGTSAIINAINWLESSSWDGRDAIVVAGDIAIYDKGAARPTGGCGAIALLIGPDAPIVFDPIRGSFMEHAYDFYKPDFTSEYPVVDGHFSLACYVKAVDQCYKNYSKKYTKDSGKTVGVYDHFDYNAFHVPTCKLVTKSYARLLYNDYLANPEKFASLIPEETRAAIDALTYEQSLVDKTLEKTFVTLAKEETKSRVQPALQVPTNTGNMYTASAWVSLASLLYYVGSENVQDKRIGIFSYGSGLASTLLSVRVVGDISKITKVLDFDYKLGEGRKIESPEQFIAAIALREAAHLQKSFVPTGSIENLSQGTYYLEEIDDKFRRKYAVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJA6</accession>
    <name>G3AJA6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">U3 small nucleolar ribonucleoprotein protein IMP3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">U3 small nucleolar ribonucleoprotein protein imp3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_59993</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374149.1">
        <property type="nucleotide sequence ID" value="XM_007374087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJA6"/>
    <dbReference type="FunCoup" id="G3AJA6">
        <property type="interactions" value="508"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJA6"/>
    <dbReference type="GeneID" id="18873169"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59993"/>
    <dbReference type="eggNOG" id="KOG4655">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097281_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJA6"/>
    <dbReference type="OMA" id="FRIKHEQ"/>
    <dbReference type="OrthoDB" id="10248812at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034457">
        <property type="term" value="C:Mpp10 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140691">
        <property type="term" value="F:RNA folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000006">
        <property type="entry name" value="U3 small nucleolar ribonucleoprotein IMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF1">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="11">
        <location>
            <begin position="4"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW34565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="183" mass="21649" checksum="5254CDC93ABED10D" modified="2011-11-16" version="1">MVRALKHHEQKLLKKVDFLDWKQDQGHRDTQVMRTYHIQNREDYHKYNRICGEIRKLAHKLSLLQPTDPFRIKHEQLLLEKLYNMGILATKAKISDLENKVTVSAICRRRIGVVMCRLKMAETMSDAVMFVEQGHVRVGPNIITDPAYLVTRNLEDYLTWVDTSKIKRNVLKYRNKIDDFDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJH1</accession>
    <name>G3AJH1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar protein 14 C-terminal Fig4-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_148054</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VAC14 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373458.1">
        <property type="nucleotide sequence ID" value="XM_007373396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJH1"/>
    <dbReference type="FunCoup" id="G3AJH1">
        <property type="interactions" value="904"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJH1"/>
    <dbReference type="GeneID" id="18870773"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_148054"/>
    <dbReference type="eggNOG" id="KOG0212">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007740_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJH1"/>
    <dbReference type="OMA" id="QCYQHVS"/>
    <dbReference type="OrthoDB" id="5574975at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070772">
        <property type="term" value="C:PAS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006661">
        <property type="term" value="P:phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026825">
        <property type="entry name" value="Vac14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021841">
        <property type="entry name" value="VAC14_Fig4p-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16023:SF0">
        <property type="entry name" value="PROTEIN VAC14 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16023">
        <property type="entry name" value="TAX1 BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12755">
        <property type="entry name" value="Vac14_Fab1_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11916">
        <property type="entry name" value="Vac14_Fig4_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar protein 14 C-terminal Fig4-binding" evidence="7">
        <location>
            <begin position="619"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="299"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="299"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="341"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="354"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="888" mass="101176" checksum="2D0E3FB7188C385C" modified="2011-11-16" version="1">MSEGKILDASLIKDLSNHIYEKRKATAFQIETLTKAALSRNDSQTIYRIINELTELTNSGTNSAKMGAITALGSVSVALGSFAIAYFLEEIIKPIFATFRDTDARVRYYACESLYNIAKIARGEILLYFNEVFDILCILVTDSESSVKNAADILDRLIKDIVSAKSTNYVSILQQDQEDQQNEIKSHLVDAQGVAIQVNNPQDPQKAFSLPKFIPSLLERMYTIDPFAKKFLLSWIELFDDIPSLELISFLPNILEPLIKFLFNNSPSDVRLETENLLNVLLKEIKSIAKVKYQVKKKQLEQRKKDGDEGDSKDVSEDTKDEKTQEEAKPKEDQQEKEANQDELEEEEDEDASIKSSDTTVIRTTPQTKSEPKNPEGEVFLIGQDIFIDHTKIIHILLSFLKSFENETKNEFANEPHEIYCQVQFIALKWLREILDISPTGILRLFPDTLSIVMKNIAITDSERDFELRDSFLNFSLALQEFLNKFNHQNGNREEDLILGITQEVSDDFNEVQLPKTINAIIKQFLGSTNELSRITSLQWLIFIYSRTPQEFLEVFVSPSTTFDLTDLLKYSNDSSNEVIFKVLQLLAKISESNQEFFKSFIIKLIKLFEQESHEKSHRVKVDFIIRKLCVSLDSEIIFTTLSEVLTTMVDDDLEFLNMMIVTLNNILLTAQELLNFRKKLKNLDVYKLEDWQLFASLFQSWCHNAPSALSLCLLTSNYELAFLIIKNLSESEVTFQLLTQLDVLIQLLESPIFLKLRLQLLEPEKHPYLYKTLYGLLMIIPQSSTYTTLRNRLTTVSSLTHTGMIATAATAGSSTSVLTTPVSTPSAGVVSTTGAITSQLSIKRKRIYEMLDKFTKVQDKHEEYTTNKVGYQESNYEGSIAAVNSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJQ6</accession>
    <name>G3AJQ6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_65155</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373541.1">
        <property type="nucleotide sequence ID" value="XM_007373479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJQ6"/>
    <dbReference type="GeneID" id="18875024"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65155"/>
    <dbReference type="eggNOG" id="ENOG502RPZX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_435446_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJQ6"/>
    <dbReference type="OMA" id="CIVANPS"/>
    <dbReference type="OrthoDB" id="4017834at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="523" mass="60207" checksum="70634BB7B20C8783" modified="2011-11-16" version="1">MSKHAVDSIIIVPTDEIVEKYHSYTEILVKGMPEKCVPDTITFTEEPNGEDFPPFEVERVPLSVQFMYHNSAPATVSSATGNPQVLVTTLDKLLQIYDNEESRSDLASVKFLAVDNSSLFFKCTDINGISLIGQRENNGHFYNHLTLLINNLQQLKARTLYKETVTRLELVEHEYHRNHDVTNTKNHTKLVMAKDPTFADPGLYKRLSWELDFLNNQEIQFCFTMTPEHVHKSIITMNQNEKLKQEIESLKLNGEFEQVETLEKSLAKRQSRNDTYVGIFSDILNYEYQYQDVYKPRRFKTSGTFPLSSKKLTGYFTMLKGEELIEYEFVHNLPQSHEVRELLELASRRSLKSTTKQYLHKRKPKSISHFNNLIFKIMARLSEINSEPAIIVVPTYIDTQQLVNQLGESYTEYNTTSVTKNIVMTAKQFIGVDVQTHNVIIADIDSLIHQTALTSGSSENHEKIPGIEDPYGDLFYSFVWKLLATQEQPNIIFGLSSKDEESIRQLFSFNNVSSVIEVNRFIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJR7</accession>
    <name>G3AJR7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_59379</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373552.1">
        <property type="nucleotide sequence ID" value="XM_007373490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJR7"/>
    <dbReference type="STRING" id="619300.G3AJR7"/>
    <dbReference type="GeneID" id="18872888"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59379"/>
    <dbReference type="eggNOG" id="ENOG502RJBG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_710016_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJR7"/>
    <dbReference type="OMA" id="VCCSVVE"/>
    <dbReference type="OrthoDB" id="4019110at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="404" mass="46228" checksum="FA11EAD0586FC1E6" modified="2011-11-16" version="1">MAQFRPYGGHEQRVVSDLSRFDYEGAGIPLRKPSVTPSEISSSKLTLDTIIPLYSSRIDERDNRGYSPLPTVETPAEHEHDLSSLPEVIDPPTPKSLVRIRKKHISVGVQVPQDNPNLLVGPTPPPKESVRPPRIPENVPENDLPPMPQPQAEKSRAQFADAEVGSLSSKRKQLEQDLIKSVMNRPLVKFHADRFGPDFAGKEITISVNFFIYLFEIMFCIIEIVISSVLLDNDSMIPVDVYRYFIADGVMGLIISLLLIFQAITYERRNGSFYCLAVTIMKLVAFILIVSVVFPSPNYAYFQIWELRRAVGAFIIISMFLWLTNLIMFVTTLYISRLNLLQELNFDYSDQGLDDKFNKRGIVTEKEEDLKEFYLNENGEMFALTEDWEKEQYKGRNKILVYTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJU5</accession>
    <name>G3AJU5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mug135-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_59405</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0612 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373580.1">
        <property type="nucleotide sequence ID" value="XM_007373518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJU5"/>
    <dbReference type="GeneID" id="18872903"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59405"/>
    <dbReference type="eggNOG" id="ENOG502REFB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2628745_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJU5"/>
    <dbReference type="OrthoDB" id="5297016at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013902">
        <property type="entry name" value="Mug135-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08593">
        <property type="entry name" value="Mug135_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mug135-like C-terminal" evidence="2">
        <location>
            <begin position="14"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="78" mass="8663" checksum="6008B10001D96F41" modified="2011-11-16" version="1" fragment="single">MPGTSTASAIPRSRMITFPDGCTAEDYGLTPIRRLADIDLLCRFEVIQYLEALTIAYNIMSSEMVLKKILKNAIAPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK35</accession>
    <name>G3AK35_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Stb3p</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_60188</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374361.1">
        <property type="nucleotide sequence ID" value="XM_007374299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK35"/>
    <dbReference type="STRING" id="619300.G3AK35"/>
    <dbReference type="GeneID" id="18873264"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60188"/>
    <dbReference type="eggNOG" id="ENOG502QW7S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020170_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK35"/>
    <dbReference type="OrthoDB" id="5391991at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000432">
        <property type="term" value="P:positive regulation of transcription from RNA polymerase II promoter by glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018818">
        <property type="entry name" value="Stb3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28164">
        <property type="entry name" value="PROTEIN STB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28164:SF1">
        <property type="entry name" value="PROTEIN STB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10330">
        <property type="entry name" value="Stb3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="521"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="425"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="450"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="353"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="549" mass="61220" checksum="83C01BE373E128C2" modified="2011-11-16" version="1">MTSNIRQHPISFASILNSNPPTPPPQSPLNYIKPEESPQKLSPPSVNLPPHFMSHSASVPPFTKQQQQQHQTMDHQFTPPSSSLSSPPLPPLKHSPPSKSKKILLSTSSPEGIQVASKITPTRLANLLIRKGPLPIRHITSQLALEVPSFDLLSLSKQRRLIMAAMEQTDLVNNVVFEKIGWGQWAVRKVDSDYIVTEGTEAGNYTHPTEDTDRKMINVNDLRNQTNLKLGWSKKKKKKQSTKPDPRRESITNHTRNLHDLKIPTEPLDSNAIESDSEDEYALSDHIDEDLSSEEEEEEEEEEDEDVFTFEDESTTTTNKFKPSPPIKFAKRVPIKFSPPPDGTSSSSRRKSSSSQPSTSAAISKPTYRHQIFPRSRLSSLENLDNYIVSSAKNSTVSINSPPTAAAPIPAISFSYSGSNSVLSASPANSWTSGGPSPSGNYIHHQSMDTISPERGNGSTATNRRKSSFNESHVRSTLINQKPAQHSAVPSSKLAHDEANANMRRMLKGKMKLMKKTRASLLLWFILCRYKNQVFYFFFFLILLLILVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK37</accession>
    <name>G3AK37_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">assimilatory sulfite reductase (NADPH)</fullName>
            <ecNumber evidence="4">1.8.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_136605</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hydrogen sulfide + 3 NADP(+) + 3 H2O = sulfite + 3 NADPH + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.2" evidence="4"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374363.1">
        <property type="nucleotide sequence ID" value="XM_007374301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK37"/>
    <dbReference type="FunCoup" id="G3AK37">
        <property type="interactions" value="434"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK37"/>
    <dbReference type="GeneID" id="18869986"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136605"/>
    <dbReference type="eggNOG" id="KOG1158">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003662_1_0_1"/>
    <dbReference type="InParanoid" id="G3AK37"/>
    <dbReference type="OMA" id="MIVAVNW"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009337">
        <property type="term" value="C:sulfite reductase complex (NADPH)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004783">
        <property type="term" value="F:sulfite reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06207">
        <property type="entry name" value="CyPoR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.990.10:FF:000010">
        <property type="entry name" value="Sulfite reductase [NADPH] flavoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000011">
        <property type="entry name" value="Sulfite reductase flavoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF109">
        <property type="entry name" value="SULFITE REDUCTASE [NADPH] FLAVOPROTEIN COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0288">FMN</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="15">
        <location>
            <begin position="712"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="635"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="635"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW32848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1095" mass="121007" checksum="BD44D3A03A85DF42" modified="2011-11-16" version="1">MAPHALAETPAVSSLDSSVNELDISSTAIIKANPFGTAVDPNLIKGAVYTSASTIINQAVYSIASKIFSYETVGADNLIDSHIQLWSQTYQRGNSFGIVPFFHKLQVRSGASNAILGYFKKNGTNGQPLSVFVGGNGLNYMRSSLAGQLNDSLPLSLNVSAIDFVEDSLVSNYGSSLSVARDLGFPVFTPVDSKDGLELQHLAVLNHTFASFVGKPSLFLFDGPDFVKSFTRYSNLLSVEKLGHLYQGLLNDLSSVVPTSVDNAIDLAFNALNNLTGKSYAPFEYVGSANPETVFIIYGSHESTQVSDLIKRSGDKIGLVKVRVPLPFNHGKFLATIPSSTKKLVVLSPDNVSLKADITAALFLNGRYFGITIDEFVYSLDFTWTPITIAKILSEFVPNLNVDKLLLSTAKNSVEALTANSSPEGQFVFWGKDNGIITTTADKLALSLSLDNSKAVDIRNRYDNSQAGGVFQSQIIVTSEGSTVGDGADVVIIEDLALLDNYDILATVKPGAKVLYINNKKIASLEEDVVAKLPLEFKKTLASNQNELILVDFSIIDTLDELNNSIKGLSGEFLLQLAFWRASLPELKGFIVNKLLQANGNSFELLATVLDNFINIVDDNQGIKEIVVDSKWSELVEEDPEEKEEQQEEEEEEEEVKQLPFFVKETSLFPNPRTSTETAEEINTNAHTSLAQKIAFPEAYNVSQELRPDLPMKNFVVKVQENKRLTPTEYSRNIFHIEFDITGTGLTYDIGEALGIHGRNNSEAVEEFLQFYGVDGDLLVEISNGAELQIRSARQVLSEMVDFLGKPPKRFYESLAEYATDETEKTAIAKLATGGEELKQRQEVSFDTYFDILQEFKSAKPAFQDLIKIISPLKRREYSIASSQKIHENAIHLLIVVVDWVDSKGRLRYGHCSKYLSDLKIGDELVVSVKPSVMKLPPLSTQPIVMSGLGTGLAPFKAFIEEKIWQKNQGMEIGEIYLYLGSRHKKEEYLYGELWEAYKDAGVLTHIGAAFSRDQPQKIYIQDKIRDTIEELTDAIVTKNGSFYLCGPTWPVPDITQCLEDIVANGAKKQGVEIKDVAKVVEDMKEEGRYILEVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK65</accession>
    <name>G3AK65_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_150215</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374391.1">
        <property type="nucleotide sequence ID" value="XM_007374329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK65"/>
    <dbReference type="FunCoup" id="G3AK65">
        <property type="interactions" value="92"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK65"/>
    <dbReference type="GeneID" id="18870858"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_150215"/>
    <dbReference type="eggNOG" id="KOG0624">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027661_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK65"/>
    <dbReference type="OMA" id="KNCLRID"/>
    <dbReference type="OrthoDB" id="1726119at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051727">
        <property type="entry name" value="DnaJ_C3_Co-chaperones"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44140">
        <property type="entry name" value="LD25575P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44140:SF2">
        <property type="entry name" value="LD25575P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442190" description="J domain-containing protein" evidence="5">
        <location>
            <begin position="19"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="6">
        <location>
            <begin position="477"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="438"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="542"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="438"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="553"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="606" mass="70506" checksum="4ECB0E392CA53D8C" modified="2011-11-16" version="1">MKILPLIVLLQLLVAIRASSDVTLEKIQEVDRLFSTNGPSSEVLHQFERVLKEVSDVPEAKPYLTQLYFKKALIELNLGKENSAVDDLKMVVTLDPLQRTAKDKLVDILVSRGDFNGLQPYLDAREDQDIYVTMNEWNQSMTLARAHYSNNDYQSCIDELNREVLGVTPNNYESGELHYLSALELFKLDPLKEFYYMEDALPLHKIIILDLQKLLNIRPWSNLNLYARISQFILFTENRFENARNVITNCVRFDNEFKPCTELSKFYTKYKDFLKLLEQYSIITGHYYANEQTSMDEEITNPNIDYRFIYDFLFVQDLKVTRMEKNKLNSSIKTNYDYLVYRASQFLQDLVGDDNYLQQLIFVQDLQKLACESYVQIEGKSGNYCKQLDDSFFPKSLPEIDKLLKQKKFNQAQRILNKFNSNVKKTKMFTDRMQPIEQYHQQQRQQQRQQQQRQYQQHFNNQRQRQFRQPQSKPLNDYYKVLGVSRDADDREIKKAYRQLTLKYHPDKYKGDDLTPEEIENKMQDINQAYEVLSTKELRERYDRGDDPNDSSPGGGGGGGSPFGGFNFNGGGAGGAEQFFQFFGGQGGGSRGFKFGNGGGFKFHAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKC9</accession>
    <name>G3AKC9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60928</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375103.1">
        <property type="nucleotide sequence ID" value="XM_007375041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKC9"/>
    <dbReference type="FunCoup" id="G3AKC9">
        <property type="interactions" value="387"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKC9"/>
    <dbReference type="MEROPS" id="M24.A10"/>
    <dbReference type="GeneID" id="18873638"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60928"/>
    <dbReference type="eggNOG" id="KOG2413">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011781_2_5_1"/>
    <dbReference type="InParanoid" id="G3AKC9"/>
    <dbReference type="OMA" id="WTDSRYH"/>
    <dbReference type="OrthoDB" id="9995434at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071281">
        <property type="term" value="P:cellular response to iron ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01085">
        <property type="entry name" value="APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000007">
        <property type="entry name" value="Xaa-Pro aminopeptidase P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033740">
        <property type="entry name" value="Pept_M24B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032416">
        <property type="entry name" value="Peptidase_M24_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050422">
        <property type="entry name" value="X-Pro_aminopeptidase_P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763:SF6">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16189">
        <property type="entry name" value="Creatinase_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16188">
        <property type="entry name" value="Peptidase_M24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="6">
        <location>
            <begin position="192"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24 C-terminal" evidence="7">
        <location>
            <begin position="417"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="483" mass="54648" checksum="E92B4F7139C6073F" modified="2011-11-16" version="1" fragment="single">NDKSIEIELTAVEDNLINKIWEEFEELPASPASNEIKSLDISFTGQETDDKIAAIKSACFKNNVEGIVVTALDEVAWILNLRGQDIEYNPVFYSFMIITKDVTKVFVDEWRLSAEIKTKLKANNIILESYGSFYSELNTISKQFTVNNKSFFIPDNANWEVVRNLKCAFVQKLSPVEELKGIKNEVELQGAKIAHLKDGRALVKFFAWLEDQLINKQELIDEIDADEKLTEFRKQEDNFVGLSFATISATGPNGAIIHYKPTKGQCATINPSKIYLNDSGSQFLEGTTDTTRTIHFETPTSEEKRNYTLVLKGNIALSTLRFPENTTGNLIDSIARQYLWKYGIDYGHGTSHGIGAYLNVHEGPIGIGPRPNAAANALKPGHLISNEPGFYETGEYGIRLENVMFVKDSGLHYNGRNFYEFETITRVPFCKKLIEVSMLSEEELAWLNNYHATIWDELSGTFEKDSIVYKWLRRETEPLALST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKJ9</accession>
    <name>G3AKJ9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60939</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375118.1">
        <property type="nucleotide sequence ID" value="XM_007375056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKJ9"/>
    <dbReference type="GeneID" id="18873646"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60939"/>
    <dbReference type="HOGENOM" id="CLU_1939448_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKJ9"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="130" mass="13951" checksum="3EF934D999F49D2C" modified="2011-11-16" version="1">MSGQNIELKSVFNKDGNYLGYYHLIPSDHLILAGSSPCCIDPLVYDGSGNLIGILNQNYVQGPNNLQPGMKDVGVVVNNEEESDNSNTKHPKLCKGLENMVRLGNTIKPGVVALGLCTTVFYAIVTAIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKR7</accession>
    <name>G3AKR7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Flavin reductase like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_60303</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the flavoredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374486.1">
        <property type="nucleotide sequence ID" value="XM_007374424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKR7"/>
    <dbReference type="GeneID" id="18873330"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60303"/>
    <dbReference type="eggNOG" id="ENOG502QT1K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059021_3_0_1"/>
    <dbReference type="InParanoid" id="G3AKR7"/>
    <dbReference type="OMA" id="GNLIICE"/>
    <dbReference type="OrthoDB" id="10250990at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33798:SF5">
        <property type="entry name" value="FLAVIN REDUCTASE LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33798">
        <property type="entry name" value="FLAVOPROTEIN OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00903">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin reductase like" evidence="6">
        <location>
            <begin position="95"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="297" mass="33230" checksum="497A9930A106ED31" modified="2011-11-16" version="1">MTNAPVATTTTPAEPKRFGLEDQVKRNPHPDFNKVENSRPGFETETAWKYTQIPNKDWKVGSGANDDSWKAHKKLEIDPYGEGRLPVDNYKTLISAVVPRPIGFVSTISKDGVRNLAPFSYFTIVNHDPPIFTLGFSGGRGNPKDTCQNILDTEELTINIISEWFVEAANFCSINAPIDVDEWKLSGLTPLPSSKVKPAHVAESAFSIEAKLIHSHEWKSKADENRATGVLCIVEGINFHVREDVINKDLNNLDISKLKPVSRLGGITYGRTIQGYEKLRPDFSKEEEKEEVKQLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALC0</accession>
    <name>G3ALC0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_50076</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374678.1">
        <property type="nucleotide sequence ID" value="XM_007374616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALC0"/>
    <dbReference type="GeneID" id="18871400"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50076"/>
    <dbReference type="eggNOG" id="ENOG502RQ9U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_924912_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALC0"/>
    <dbReference type="OrthoDB" id="4096449at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="301" mass="34936" checksum="6A67E1A82386AB8B" modified="2011-11-16" version="1">MKLARGVAKLSLSSIQASLQSSDNDEREIIKHIDYDMLERAYNIHKYYPQYIHNYDISTPVLRMLIVDKPIYNDANQHLKNGESYEKRKLLNCQLHEKRDARKASRKERKERKREKQLMKQEQKLKNKGYHDQFKNEDSDYYSDSTDVDIDEQIITGKAELVSIPLVKDSTTWSESSNTRAESTRTMSKNKLITKSHTHPTSKHNSFGFKILNKMFPKSPPGTKNSADAPMSKMISLQPIDKSSKSEIIIPLQKANTFEQTKSNLTLSRRLAKRLTRSDQENPNVIGMYTFFYFILCSSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALE6</accession>
    <name>G3ALE6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mediator of RNA polymerase II transcription subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mediator complex subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_71074</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the Mediator complex subunit 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374703.1">
        <property type="nucleotide sequence ID" value="XM_007374641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALE6"/>
    <dbReference type="STRING" id="619300.G3ALE6"/>
    <dbReference type="GeneID" id="18875358"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_71074"/>
    <dbReference type="eggNOG" id="ENOG502RYK5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_479935_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALE6"/>
    <dbReference type="OMA" id="WQDNRYI"/>
    <dbReference type="OrthoDB" id="5310959at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019680">
        <property type="entry name" value="Mediator_Med1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35041">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35041:SF4">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10744">
        <property type="entry name" value="Med1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med1" evidence="8">
        <location>
            <begin position="9"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="568" mass="65481" checksum="605074199E412434" modified="2011-11-16" version="1">MVSFNQSLDTSLQLLYDYSSNFQISVELVQKLAQHLKLETFIDTSFQFGNVEGNNQQRLTIAASSILLDIDFENDRKVSNISLSIGNESTHTTENSKEYIESVQNNHGITVIKLNPNKNPLTFLKKSSDEKSIAEKILLANLSQPKLNKFPENLQYLAIIDKYANSNQDLFTYIERTALLLHTISQLEIERNPNNWQIEQGLNNAIGNVKIHDITQGKIGLFLEFWKDFRYINHESNTAIGNTYNVQFNIVSSSYNHEYLLENNKKEWEIANQGNKERVKFEFEDAVIPTVNNDGTNTTMLPWMFDLVLNHPIYVPKYILEYLGLDYTVEENTTDPLFKIFDKINNSQEVVDSINILGRNVSCLINHDLISNFIPLKSVKINHLYDLSSLIPILRNFIVLSNLYRTLSQNNCIIPRRKSRRRSSRVSFKNDKELTEEIRKKLKDSLKLPDDVTDEELLGLNAISETATYSTIQPINDMFDLESFIKNDQTTTSKENYFQVSLDDIDVTSNDLYLTVEAYSDIKEIYIRFKISNGQIQLLQDDIDENNAKFIKTLDLTEDFVVSITSVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALP8</accession>
    <name>G3ALP8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60627</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetate uptake transporter (AceTr) (TC 2.A.96) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374806.1">
        <property type="nucleotide sequence ID" value="XM_007374744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALP8"/>
    <dbReference type="GeneID" id="18873496"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60627"/>
    <dbReference type="eggNOG" id="ENOG502QUJS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051062_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALP8"/>
    <dbReference type="OMA" id="ELQEWEY"/>
    <dbReference type="OrthoDB" id="3648309at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015123">
        <property type="term" value="F:acetate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051633">
        <property type="entry name" value="AceTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000791">
        <property type="entry name" value="Gpr1/Fun34/SatP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047622">
        <property type="entry name" value="GPR1_FUN34_YAAH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038013">
        <property type="entry name" value="AceTr_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123:SF1">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01184">
        <property type="entry name" value="Gpr1_Fun34_YaaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01114">
        <property type="entry name" value="GPR1_FUN34_YAAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="256" mass="27350" checksum="5C7C9B37572E876A" modified="2011-11-16" version="1">MSNSSMQSKDHSLDTDNDPVHQIQTAGAGNEFVIIGNKKYYRHELMEAFGGSLNPGLAPPPVHKFANPAPLGLCAFGLTTFVLSMVNAQAMGIKIPHAVLGLAAFYGGAVQFLAGIWEMVVGNTFGATALTSYGAFWLSYAAILIPSFGVGAAYEEEPEMKADAVSFFLLGWTIFTFMMTLLTLKSTVFFCALFSCLFITFLLLCIGDFTRTVGITRAGGCVGVVTAILGFYNAFAGTATPQNSYFGAKVIHLTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALQ9</accession>
    <name>G3ALQ9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_55194</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374817.1">
        <property type="nucleotide sequence ID" value="XM_007374755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALQ9"/>
    <dbReference type="FunCoup" id="G3ALQ9">
        <property type="interactions" value="1228"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALQ9"/>
    <dbReference type="GeneID" id="18871916"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55194"/>
    <dbReference type="eggNOG" id="KOG2023">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008136_1_1_1"/>
    <dbReference type="InParanoid" id="G3ALQ9"/>
    <dbReference type="OMA" id="AQEGAMS"/>
    <dbReference type="OrthoDB" id="951172at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010458">
        <property type="term" value="P:exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="8">
        <location>
            <begin position="32"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="6">
        <location>
            <begin position="454"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="331"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="331"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="351"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="370"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="932" mass="104269" checksum="9AB2B6958684A111" modified="2011-11-16" version="1">MSSWTADPQALEQLKHIFRGSLSINNQERKLANDALDQAKQEPEFENYLFELLVKDTSAKSDVRAAAGINLKNSILKRRQSNRQYLLDNILTGLTSDDSMVRNITGNVITSLFSIYGLEGWPTILPNLLALAKTSTGTGFVPQEAAMSALSKICEDSYSQLDVEFQGERPLNFLLASFLELLSHPSDKIKASAIHCVNQFVPLNTQSFLVIIDAYLTKLFSLAQDGGSNEVRKNICSSFLLILESRPDKLMPHLDGVINYCLHLMQENTSEEVSLEACEFLLALSTASEQDKELFKPKLSLILPILLDKMVYSEEEIFLMEIADTRDNADVADKDEDIKPQNAKGKDARTSNNNSVNGSANGTKNPSPDYDSDVEDDEDDEESEIDQWSLRKCSAATLDVLSENFPGEVLMVTLPILQEKIVSPEWPVREAAILAFGAISASCIELARDKLPSLVPFLVDRLQDEQPRVRQITCWTLSRYVSWVSEEAHDGGEYANYFQPTFQSIVTCALDKKKVVQEAACSALSAFIEESDSSLIEFYLEPLLAHFAKCFETYQRKNLIILYDCIQTFVEKMGYENLSSNPEYMNTLLPPLLRKWEILDDNDTGLWPLLECMASIAATLRELFAPFAVPVYERAIKILSNCIQLDQQCQTDPSIEAPEKDFMVTSLDLIDGLIQGFEQHSVDLIQQQPAHNLMELLMLCFEDYNADVRQSAYALLGDLAIFVLDPIVKPYLHSILLSIGNEINNRSYSSYPVYNNAIWALGEIVIRTTFEEVKPYLSNLVDLLIPVLNSTDTQSTVLENAAICLGRMGLTPGGAATVAPRLAEFIVSWCSHFLYLVDNNEKETGFHGMINIIGVNPDQGFGGLSNQQGKKNLAILITCIGNYQSPTQELQHLFTDLISNYRVLLGEEVWQSQVLTHIDPEVRNHLQVVYGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALZ6</accession>
    <name>G3ALZ6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60686</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374863.1">
        <property type="nucleotide sequence ID" value="XM_007374801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALZ6"/>
    <dbReference type="STRING" id="619300.G3ALZ6"/>
    <dbReference type="GeneID" id="18873523"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60686"/>
    <dbReference type="eggNOG" id="ENOG502SEBV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037642_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALZ6"/>
    <dbReference type="OMA" id="GIKDCLP"/>
    <dbReference type="OrthoDB" id="429932at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005640">
        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070762">
        <property type="term" value="C:nuclear pore transmembrane ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030474">
        <property type="term" value="P:spindle pole body duplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012578">
        <property type="entry name" value="Nucl_pore_cmplx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28003">
        <property type="entry name" value="NUCLEOPORIN POM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28003:SF1">
        <property type="entry name" value="NUCLEOPORIN POM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08058">
        <property type="entry name" value="NPCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="459" mass="52286" checksum="5BCB15888EA6F457" modified="2011-11-16" version="1">MATRNIDDANNANTNTYGSRYVSKYSDYLGASSKQALVSSSLKKLPDNSMSSIDFHTSIPTGSFVEEAHERDIYRPTISTTNDSSTDVNVTPLKEAQFKILQTKLSMKAKLAENEALDKEIRNLDQPIYYKPPPANYEDSYYGIKSLLPPNIKLGNKQNKENKIPIIVSKPDEAYDTSDLLKSIRDQQQENDDIDDDFHADNPAGDWASPVVKEALARQVDLEGCVVRIFKNVIFLVAFLLIKSLAKKLMYLYDLRVKSQPYYVYWNNKYESLSDNIYLIVASKILVGIFVLNIIIPLIRIIKGQDQCYDLPLSEKQRQLIGLKVRDRPSDQRGKWEGIDEVDESAELTLKQRRYNLIHKVPYKKVPKYRKLNDYSIYKAKPVEVSKSDEASIQGPSIYPSRTLSNATQLTNTKPTGLHFLADIPKPIAKSKYSQEKIVKEKAKFAKNFNIDFNIADDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM85</accession>
    <name>G3AM85_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fumarate reductase</fullName>
            <ecNumber evidence="4">1.3.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">OSM1</name>
        <name evidence="6" type="ORF">SPAPADRAFT_60747</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Irreversibly catalyzes the reduction of fumarate to succinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>succinate + NAD(+) = fumarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18281"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.3.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="4">Binds 1 FAD per monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.6" evidence="4"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374898.1">
        <property type="nucleotide sequence ID" value="XM_007374836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM85"/>
    <dbReference type="FunCoup" id="G3AM85">
        <property type="interactions" value="73"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM85"/>
    <dbReference type="GeneID" id="18873545"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60747"/>
    <dbReference type="eggNOG" id="KOG2404">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011398_4_5_1"/>
    <dbReference type="InParanoid" id="G3AM85"/>
    <dbReference type="OMA" id="MAWAHGA"/>
    <dbReference type="OrthoDB" id="10254877at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016156">
        <property type="term" value="F:fumarate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050315">
        <property type="entry name" value="FAD-oxidoreductase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010960">
        <property type="entry name" value="Flavocytochrome_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01813">
        <property type="entry name" value="flavo_cyto_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43400">
        <property type="entry name" value="FUMARATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43400:SF1">
        <property type="entry name" value="FUMARATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0274">FAD</keyword>
    <keyword evidence="1 4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="5">
        <location>
            <begin position="10"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW33383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="493" mass="53450" checksum="9CCB983E52214750" modified="2011-11-16" version="1">MPNRTGPSTIIVGSGLAGLTTAVELLAKSSGKVTILEKTDKLGGNSIKASSGMNGVPTRFHVKDSLDSPDAFYSDTIKSGKGLSDEKLVRLLVDNSGDAIHWLADEHAVILNTLSQLGGHSFPRTHRGGGKLPPGFEIITALTKKLEEIMVAKPDRLSILTNSRVTKLIADAKNPNQVKGIQYLSPDGELKTLLGDNVVLATGGYSADTDEEEGSLLKRFRPDLLEYPSSNGEQTTGDGAKLAERDVNAELIHMDKVQVHPTGFIKLSNPNSNWKFLCGEVIRGIGGILLSPNTGFRFVNELTTRDGVTDAIIKNCKVKGDNDMNLKRETAIAAIAINLVDYHKAKTHIDFYGFQKLLHKGNIDDLILFMQRLNPDLNISHDELVESFEDYNTFIRNGKDPQYGRTSFGAEFNVEHGLFYFGLVTPVVHFSMGGIKIDENGRVLTKDKTTMTNLYAVGEASGGVHGANRLGGSSLLECVVFGKQVSKDIIANA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMM3</accession>
    <name>G3AMM3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_60818</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374982.1">
        <property type="nucleotide sequence ID" value="XM_007374920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMM3"/>
    <dbReference type="FunCoup" id="G3AMM3">
        <property type="interactions" value="529"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMM3"/>
    <dbReference type="GeneID" id="18873586"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60818"/>
    <dbReference type="eggNOG" id="KOG2910">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086201_0_1_1"/>
    <dbReference type="InParanoid" id="G3AMM3"/>
    <dbReference type="OMA" id="RAKQPAM"/>
    <dbReference type="OrthoDB" id="441172at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF5">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="170"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="20"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="190"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="215" mass="24388" checksum="BC2B42C851B8D568" modified="2011-11-16" version="1">MGQSTSAPKITAQDRAIFKLKEQRDSIKQYQRKLSRVVDRQSELARQAVKNKQPDRAKFYLRSKKQQESVIAKTFDQLDNLENLIGTIEFKLIEKDVIYGLQEGNKVLTKLNNEMSVERIDKVLDQLEDERIKVDEISDALGMGSGLNKLEETEVDEELAKLNEEINGPEVVKLPDTPNTQILPKAPTNKIEDKVEEVKEEADKVGQQANEPLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMZ0</accession>
    <name>G3AMZ0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial cytochrome c oxidase assembly factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_67023</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PET100 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375680.1">
        <property type="nucleotide sequence ID" value="XM_007375618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMZ0"/>
    <dbReference type="FunCoup" id="G3AMZ0">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="GeneID" id="18875131"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67023"/>
    <dbReference type="eggNOG" id="ENOG502S1QV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156745_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMZ0"/>
    <dbReference type="OMA" id="VIFPIGW"/>
    <dbReference type="OrthoDB" id="18175at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018625">
        <property type="entry name" value="Pet100"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33968">
        <property type="entry name" value="PROTEIN PET100 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33968:SF1">
        <property type="entry name" value="PROTEIN PET100 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09803">
        <property type="entry name" value="Pet100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="12238" checksum="CBA69EEEF6ED7C1B" modified="2011-11-16" version="1">MASRYQRFIHRITRTQLETAKFGWYLLTPICIMYYVGLDTDTKFNLPGFWPDPSTLNQVPKEPHEIQAELARIKRARAEKRRKLEERAKELGITEEEVQSSEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN57</accession>
    <name>G3AN57_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL14m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_60997</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375176.1">
        <property type="nucleotide sequence ID" value="XM_007375114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN57"/>
    <dbReference type="FunCoup" id="G3AN57">
        <property type="interactions" value="418"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN57"/>
    <dbReference type="GeneID" id="18873674"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60997"/>
    <dbReference type="eggNOG" id="KOG0901">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095071_2_0_1"/>
    <dbReference type="InParanoid" id="G3AN57"/>
    <dbReference type="OMA" id="IVCVVQK"/>
    <dbReference type="OrthoDB" id="274765at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000005">
        <property type="entry name" value="50S ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005745">
        <property type="entry name" value="Ribosomal_uL14_bac-type"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_uL14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01067">
        <property type="entry name" value="rplN_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L14/L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="129" mass="14253" checksum="8A0F014391BD2305" modified="2011-11-16" version="1">MIYLKSLLNVIDNSGALVVECIKVLRHKPKSCAQIGDQITVVVKEAKPIADGTTSTNKVKRKDICRAVVVRTRSPFRRPDGSMVKFDDNACVLINKNGEPLGTRISSVVAKELRDLQYNKIVSLAPKTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN80</accession>
    <name>G3AN80_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cysteine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cysteinyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_55908</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="2"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375739.1">
        <property type="nucleotide sequence ID" value="XM_007375677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN80"/>
    <dbReference type="FunCoup" id="G3AN80">
        <property type="interactions" value="946"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN80"/>
    <dbReference type="GeneID" id="18871984"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55908"/>
    <dbReference type="eggNOG" id="KOG2007">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013528_3_1_1"/>
    <dbReference type="InParanoid" id="G3AN80"/>
    <dbReference type="OMA" id="FHNDMKS"/>
    <dbReference type="OrthoDB" id="438179at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1910">
        <property type="entry name" value="Cysteine-tRNA ligase, C-terminal anti-codon recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890:SF3">
        <property type="entry name" value="CYSTEINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA synthetases class I catalytic" evidence="12">
        <location>
            <begin position="42"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="689"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="689"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW32463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="763" mass="87174" checksum="55F67B3D71D9EDCA" modified="2011-11-16" version="1">MATPIKKTPVKQPEWFAPVNKNDQKPTLKIYNSLTRSKEDFHTIRPGHISWYSCGPTVYDHSHMGHARNYVSTDICRRILQDYFGYNIKFVQNVTDIDDKIIIAARQQYLFEQKVSNVYTQLNDELLQNSKRFLSSYIEKNLPEFTGSIESEFDQWSKTINTQSNYETLVKEKPKLPMYVKAATVAHQAVYSKAPGSIEEFLAAIKDTAMPYLDSQYGSTVTDPEIFRKLPAYWEKRYNEDMARLNVLPPTVTTRVSEYIPDIVAYVDRIIENGYGYRTDDGSVYFDTAKYEHSHHDYAKLQPWNKGDMSLINDGEGSLTAGNAKKNPSDFALWKASKPGEPSWESKWGKGRPGWHIECSVMASDITGEQMDIHSGGIDLCFPHHDNELAQSEAYYDNRQWVNYFMHNGHLHIQGQKMSKSLKNFITIDEALRDFSSRQLRLVFALSPWDKPLDFKDSLINEVKSIESSFSKFFTNVRALDSDYKHTVEEGGFVSRKITEVEKQLYDDLAIAQDEVDTAFNDNLSSGQALRAIQELISKANNYIQLATLNKVELRIEVLLAITNWIVKILNILGFETRPDRLGWNDSTGGNNGDAASTEDIALPYVKALSQFRDSVRGLAIAKADMKEILNACDSIRSQLVNLGVSLDDRPNGSALVKFLNEQEKQQLIEQQEAKAKAIAEKERKKQEQALAQAKKEQERLEKMKTPPSELFKDSSLYSEWDQDGIPVTDAKGEKISNSMRKKLTKQFEKQKALYDEYLKSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANC3</accession>
    <name>G3ANC3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_51056</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375782.1">
        <property type="nucleotide sequence ID" value="XM_007375720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANC3"/>
    <dbReference type="GeneID" id="18871509"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51056"/>
    <dbReference type="HOGENOM" id="CLU_947205_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANC3"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="294" mass="34085" checksum="C428AE63BAC468B4" modified="2011-11-16" version="1">MTLVPNSKGSEGSSDIVQGDLDKEDVESMFQKLDYLEPSHDANSITAEEETIVENMGSNVIAKLDILEKGTVEYSKLKKDIFVIIYECILGTYLWNDVWKKSFEQVLECCCAFYGWRLESINQEHNTSLPKLHVSQLSDIVLESYFPADVFSCSVTLLTGTIDVHWWKWIYMKLNFRVNASQWLKMELKEEGTNTYDNFGFFSDHEFNCFIWSLFVWFLVVDLNISLLDKQNNKDLDMNFVANLHDDSFQVYENLEEYEAESLFRKLGLEPATDDHIEAVLLASTDYEAFKCHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANF3</accession>
    <name>G3ANF3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">D-aspartate oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_55523</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DAMOX/DASOX family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375218.1">
        <property type="nucleotide sequence ID" value="XM_007375156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANF3"/>
    <dbReference type="STRING" id="619300.G3ANF3"/>
    <dbReference type="GeneID" id="18871944"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55523"/>
    <dbReference type="eggNOG" id="KOG3923">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034311_1_0_1"/>
    <dbReference type="InParanoid" id="G3ANF3"/>
    <dbReference type="OMA" id="PGMREPK"/>
    <dbReference type="OrthoDB" id="2015447at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003884">
        <property type="term" value="F:D-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006181">
        <property type="entry name" value="D-amino_acid_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023209">
        <property type="entry name" value="DAO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11530">
        <property type="entry name" value="D-AMINO ACID OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11530:SF11">
        <property type="entry name" value="D-ASPARTATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000189">
        <property type="entry name" value="D-aa_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00677">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="7">
        <location>
            <begin position="4"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="46"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>D-dopa</name>
            <dbReference type="ChEBI" id="CHEBI:149689"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>D-dopa</name>
            <dbReference type="ChEBI" id="CHEBI:149689"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>D-dopa</name>
            <dbReference type="ChEBI" id="CHEBI:149689"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000189-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="345" mass="38799" checksum="47E7415ADB98F6F4" modified="2011-11-16" version="1">MTKYVIVGSGIIGLYTTFQLLEHHIAPHEITVIAEYMPGDESINYTSPYAGGNFSCITGDDPDTLEFDKYTYLNLHRIQKVLGKEAGLDRYPSVELWDRRPAPEKIESLSSYLDQYDVITEKDELDGAEFGIRFLSWNFNCPKFLANFQKYLEKQGVRFVKKRLSHIVQAYGPSTKTVINCSGIGARTLGGVEDKQVYPTRGQVVVIKAPHIMENKMRWGDDYATYIIKRPYSHDQLILGGFIQKDNWTGDTFKSETEDILNRTTTLLPKILLKNPGGDKVEDLEILRVAAGLRPSRHGGARIERESFDEGKVLIHNYGASGYGYQAGLGMAYKAVQLAVAKSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANG7</accession>
    <name>G3ANG7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribose 1''-phosphate phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_55534</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ADP-alpha-D-ribose 1''-phosphate + H2O = ADP-D-ribose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25029"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="ChEBI" id="CHEBI:58753"/>
            <dbReference type="EC" id="3.1.3.84"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the POA1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.84" evidence="2"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375232.1">
        <property type="nucleotide sequence ID" value="XM_007375170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANG7"/>
    <dbReference type="FunCoup" id="G3ANG7">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="GeneID" id="18871946"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55534"/>
    <dbReference type="eggNOG" id="ENOG502S60W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054419_1_2_1"/>
    <dbReference type="InParanoid" id="G3ANG7"/>
    <dbReference type="OMA" id="CQGSWGK"/>
    <dbReference type="OrthoDB" id="2155246at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047407">
        <property type="term" value="F:ADP-ribosyl-[dinitrogen reductase] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140291">
        <property type="term" value="P:peptidyl-glutamate ADP-deribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02901">
        <property type="entry name" value="Macro_Poa1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050892">
        <property type="entry name" value="ADP-ribose_metab_enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12521:SF0">
        <property type="entry name" value="ADP-RIBOSE GLYCOHYDROLASE OARD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12521">
        <property type="entry name" value="PROTEIN C6ORF130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="5">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW31956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="160" mass="17682" checksum="B6E95F03C2215FF6" modified="2011-11-16" version="1">MIKYIKGDLFSHKSIDISKSVILAHACNTHGAWGGGIAAVFGRQFPTANTKYSNYCHTHSNLLGTTLLIPANDNNKIFIACLFTSDFSQTPTEIVEYTDKALEDLSKQLASSDLDFETDQDHPIINMPKINAGIFGVPWELTEGALMKHTSLNFNVYVID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANI9</accession>
    <name>G3ANI9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flo11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_67124</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375794.1">
        <property type="nucleotide sequence ID" value="XM_007375732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANI9"/>
    <dbReference type="GeneID" id="18875140"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67124"/>
    <dbReference type="HOGENOM" id="CLU_566402_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANI9"/>
    <dbReference type="OrthoDB" id="4028176at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018789">
        <property type="entry name" value="Flo11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10182">
        <property type="entry name" value="Flo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51824">
        <property type="entry name" value="FLO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442622" description="Flo11 domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Flo11" evidence="4">
        <location>
            <begin position="42"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="206"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="482" mass="53064" checksum="29F0EDF1DB08FD25" modified="2011-11-16" version="1">MLQTLFLWYLLLQMLVPANAATTTLSGPVTVSRPYTNLPKPTANYPTSWNGNSICSSSLGALSKQNNEPKLKIFEFKSIKWIEDNFYEVMIEFEIDSKAYTESELRAIYIFSLQTPNDYLGSVELFATSWQQNLLGNSPFHFYFSWVMEAENINQLTCTTPFQVYYDWDTYYATYQHGCFSDQLTDLPAQCWNAKNFVQGVSQSDTKTSSESKTTPTKETSKVGTNTNVGASSESKYKSTTEQESKSTTKPGNQSIIETKVQINTQQPVGTSSEHSHVSTSMQNPSTSDSKAPSSTKNEIQTTSKPELGISHDLEEETISLSAISIETEIIEQEISTPDSTISDTEEKDTPTVDGTATPVNDESTIEPNFEIITTVTRTTTTYVTATVCSSDCQPSSNDSVASDVNIITSDEGAYPTLVESVYTSITSNNASKTMADYIPIPGNSTNTLNANSVEMFEGSASAISTNCLLVVLYLFSNFIFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANM6</accession>
    <name>G3ANM6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Association with the SNF1 complex (ASC) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_67155</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375831.1">
        <property type="nucleotide sequence ID" value="XM_007375769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANM6"/>
    <dbReference type="STRING" id="619300.G3ANM6"/>
    <dbReference type="GeneID" id="18875144"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67155"/>
    <dbReference type="eggNOG" id="KOG1616">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_445500_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANM6"/>
    <dbReference type="OMA" id="IMHSPAD"/>
    <dbReference type="OrthoDB" id="531008at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006828">
        <property type="entry name" value="ASC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037256">
        <property type="entry name" value="ASC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050827">
        <property type="entry name" value="CRP1_MDG1_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343:SF84">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04739">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01010">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160219">
        <property type="entry name" value="AMPKBI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Association with the SNF1 complex (ASC)" evidence="3">
        <location>
            <begin position="475"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="221"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="425"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="523"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="96"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="242"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="261"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="541"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="627" mass="70196" checksum="629E4FA4BC3CE6D6" modified="2011-11-16" version="1">MGNTTSSSSNNHASNGVGLSSGSRKSSARKESTNSDVRKSTASHKVTSLDEEFSDLILHQVKPTSSQSTSISQHGYVHSSQSTLMQNQGGLGHSSVAHSQSSIAHPSSKQQPYHVPQQIRLFNPNSVSSGPMESGHSNESEQSAEYNNFTNDLIEDEFNELDDVLMETDDTSVSRNILPDEEQSEVMDVVHEEHPIEHNIYSPPPDLSKVKFTKVSNGTHGDISLPLSAHPPSFPPSAPQSTISAAQQARNAHSPSPNPEQQQQQQQQHINQQFHQKRFKSNTNSLLIPVEIKWVNTSREPISKISIIGSFSAWRDVIKLSPSPKHDNEYVTNINLPLGVHKLLYIINNEYRVSDQLPTATDQEGIFFNWFEVIDDTHLFNHSSNQPNHVGASTDYDANIIAGKSEVERIKEKSNSFLQRLSKENSNVEHVEHAEQEEQDHDQDHEQHYVYPNSYDMPYDMSTSSTSLFKQDSRISQPQLEYSSEIPEMFVNYDYFKQKAPDYELAEPPHLPPHLNNVLLNKMQPQGHPPPLTPTHSSSLIPQPTYMQPSNSTMSTTSAGSFHNRPPLRRADSSYYASSKESSIPNHVILNHLMTTSIRNDVLTVACITRYSGKFVTQIMYSPADNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANQ7</accession>
    <name>G3ANQ7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="3">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_139225</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="3"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375268.1">
        <property type="nucleotide sequence ID" value="XM_007375206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANQ7"/>
    <dbReference type="FunCoup" id="G3ANQ7">
        <property type="interactions" value="854"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANQ7"/>
    <dbReference type="GeneID" id="18870191"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139225"/>
    <dbReference type="eggNOG" id="KOG0903">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002446_2_0_1"/>
    <dbReference type="InParanoid" id="G3ANQ7"/>
    <dbReference type="OMA" id="ANYFRCE"/>
    <dbReference type="OrthoDB" id="10264149at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140504">
        <property type="term" value="P:microlipophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000786">
        <property type="term" value="P:positive regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042998">
        <property type="term" value="P:positive regulation of Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050714">
        <property type="term" value="P:positive regulation of protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05168">
        <property type="entry name" value="PI4Kc_III_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000016">
        <property type="entry name" value="PIK1p Phosphatidylinositol 4-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021601">
        <property type="entry name" value="Phosphatidylino_kinase_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057754">
        <property type="entry name" value="PI4-kinase_beta/PIK1_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF22">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11522">
        <property type="entry name" value="Pik1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIK helical" evidence="8">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="7">
        <location>
            <begin position="675"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="202"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="552"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="562"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="970" mass="109174" checksum="02D567AEAC016E85" modified="2011-11-16" version="1">MESPNEHAMLLDTIRSTSFNAFQCIHNLKNYRDHIGIQYELVRKLRTYSYDELEFFIPQLVQLLVAFETNSMALEEFLLDYSSRFPHFSLIVFWHLQAYVFELRNDPESYSFQTVRNIISKLQNIMFNAESPHMKQSEFRENIQPALVLCGAIAASVAAPRFNDYASALVKSQGKQQRSFVFKLANFQKSLTRNLTMKNTRISSDLNPVSHSDDERPVSANKSPSHHRSASLPRIVTKKNSISLSSDESEMLTDDDGDRKISIELEKELLKLEMTNTKTSPMKYSSVEENLRINTIIKSKKRNIASPITEGRSKFTSHVLNSGEVYNMNSHSLPNLSTMSESGSGPYLSLAESESTLLISPNSSEQRLTESIHIPKSSSVPTYNQLLKVLQVNYTKKETQFIMALQNISIRLSQVPKEARLSALRAELSIINDTLLPSEIDIPQLLPFTSNKNKKYHKILKLSINEACVLNSAERVPFLLLIEFLSDEIDFNPFTEYNEKIIAKRAGGIQVESKVGDFSRSSANSETEVHGSALKDIDIPVRVLTPDHEEADLGDLPMSMKRSGSSRAGVTSDGVKNNNPKILSPNPINTSEMSTKVLADQMRIASVMLQQLESAGKANSDQSLAIKARIVESMIALQDQFEYINYEQFNQLQGDVPSAGQRKLENDFKLAEDWNVKKERIRSSSAYGHLENWDICSVIAKNGDDLPQEAFACQLITMISNIWKADGISAWTKRMKILITSANTGLVETITNAMSIHSIKKSLTEISVKNGENSKGRIITLLDYYERLFGKHDSPKFKQAQDNFASSLAAYSIICYILQIKDRHNGNIMVDNEGHIIHIDFGFLLSNSPGSVGFEAAPFKLTSEYVDLLGGLDSPAYAKFVMLCKECFNSLRENSDQIIDIVELMQKDSSLPCFNNGDNTSVLLKQRLQLQLSRDEADEYVDAMLIGKSLGSMYTRLYDQFQMITQGIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANU1</accession>
    <name>G3ANU1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_50624</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375302.1">
        <property type="nucleotide sequence ID" value="XM_007375240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANU1"/>
    <dbReference type="FunCoup" id="G3ANU1">
        <property type="interactions" value="799"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANU1"/>
    <dbReference type="GeneID" id="18871465"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50624"/>
    <dbReference type="eggNOG" id="KOG2103">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_320547_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANU1"/>
    <dbReference type="OMA" id="FQVLNHK"/>
    <dbReference type="OrthoDB" id="28092at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026895">
        <property type="entry name" value="EMC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011678">
        <property type="entry name" value="EMC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573:SF0">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07774">
        <property type="entry name" value="EMC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442626" description="ER membrane protein complex subunit 1" evidence="12">
        <location>
            <begin position="19"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="823"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="domain" description="ER membrane protein complex subunit 1 C-terminal" evidence="13">
        <location>
            <begin position="642"/>
            <end position="850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW32026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="853" mass="96027" checksum="43E69AFA050D6BF0" modified="2011-11-16" version="1">MKALSSILILSLISFVHAVLLSDAFTSKEIINYLYGQLTQFELSNNTLVGVSTSDQLVGIDVKGDNAVHWQIHIHNLGSSKLSTTKEKVYIYNEESHDIYQVDSKNGALNIVTLSASPVHIADAGQGVFVVDSSGRLAYIDHATGSVSKLELEVVETIRVDSNGRFAYVIVNDSRLLKFGKGRVLFDIEFSVGSIKYFKSGIIITENDQVFKLNERKKKFTRIENDSFKNLQVINENFLYSSLDDSIKIISITKKVELVDTLHIKSASRVELLNSPLNDLIVASSSIGVKDVFDLTDFLITKNPNSIKKFTIKLSHSTSHDFLTVHEDKLALLSIDNELNGGLFSLFDGQLIKPITSHYQYYSNSNSYIIINPPESQQTIHQVHEILEVDNKYILTNWLTRVKRHLAELGKFIVSLAKNGITSNESSEDFGFTKYIVFFDDSHQKLVAIKSTGGIAWESVPIHDEFISLDQLDQDRLIAIFHGFVLVFDSSDGSIIERIRNENYTEIVKVRGTAALRGESTILLNSVSEDSFLVEKGENYISGQFIPHGEVTAKQTWKFRFGETIVNMASIDPDSTTSSVGIPLADKSVLYKYLNPNLVSVLTFDKQLKLYLIDGITGNLLHVSAHPEEEIIDLSSVKIVMDDNWIVYSYFINSPRFEQRINVVDLFDKPPMQTSNSVFDRDLSIDSVKTKSFINPERILDIASSKTIYGITTKSIIAITESGQLIEIPKFVLNSRRIDDRVLTRQDYSNDFSVIPYDPILAQPPAINHKYQLDPTGKILIKPTRFESTSVICYFNKCNQFCSVIQPSKSFDILSGSFAKGKLTITVILLFIVFVITKPFVFNKKLNRQWLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANW8</accession>
    <name>G3ANW8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein OSM2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">OSM2</name>
        <name evidence="10" type="ORF">SPAPADRAFT_61660</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375869.1">
        <property type="nucleotide sequence ID" value="XM_007375807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANW8"/>
    <dbReference type="STRING" id="619300.G3ANW8"/>
    <dbReference type="GeneID" id="18873994"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61660"/>
    <dbReference type="eggNOG" id="KOG0537">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2404">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011398_4_5_1"/>
    <dbReference type="InParanoid" id="G3ANW8"/>
    <dbReference type="OMA" id="EDLWVVV"/>
    <dbReference type="OrthoDB" id="10254877at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050315">
        <property type="entry name" value="FAD-oxidoreductase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010960">
        <property type="entry name" value="Flavocytochrome_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01813">
        <property type="entry name" value="flavo_cyto_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43400">
        <property type="entry name" value="FUMARATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43400:SF1">
        <property type="entry name" value="FUMARATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="9">
        <location>
            <begin position="570"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="535"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="544"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="558"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="654" mass="71047" checksum="9CBBBAC550225586" modified="2011-11-16" version="1">MSEPSNPIIIVGGGLAGLSAAHQAYLRGANVVLLDKQGFLSGNSGKATSGINGALTRTQVQLGIKDSVEQFYNDTLKAAKDRANPDLIRVLTYDSADAVHWLQDVFELDLSVVSRLGGASQPRTHRGHDSKFPGMAITYRLLERLESLSETEPDRVQILKHCQVIDLLIDDNDKGKVVGVKYKNLQDKSKNEVHGPVIMSTGGYAADFTKNSLLRKYRPDIIDLPSTNGNHATGDGQKIIMKNHGVGIDMDKVQVHPTGLIDYRDTDVVMGKKTPRFLFLGAEALRGEGGVLFNSKGERFVDELGTRDWVSGEMEKQFKQGNGPIRLVLNEASGKNLEFHVKHYSSRNLMRTITGKELVAELGCTEESFKQQCDTYNKALRGESPDPFGKKYFPETPFEYDPNAKYHVAFVTRVLHFTMGGVKINDKAQVLTESDKPFEGLYAAGEVAGGVHGHNRLGGSSLLACVVYGRLAADQACSYSLRKLSTGVVSGSSTNSIGATTANSRLNMIGLHIDPNGKIVIELSANKTGVTSQVNEVSPGQVQPAELTPAPAATKPASKPEPKKPFSVPEREFTAEEVAKHNTADDCWCIVKNVVLDCTPFLKYHPGGAESILNFAGKDASESFAMLHEDNVIPKYVASHVLGRLKGETPCLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP16</accession>
    <name>G3AP16_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">COPII coat assembly protein SEC16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61142</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375323.1">
        <property type="nucleotide sequence ID" value="XM_007375261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP16"/>
    <dbReference type="STRING" id="619300.G3AP16"/>
    <dbReference type="GeneID" id="18873738"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61142"/>
    <dbReference type="eggNOG" id="KOG1913">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_893001_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP16"/>
    <dbReference type="OMA" id="EDGRWFN"/>
    <dbReference type="OrthoDB" id="8918678at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01765">
        <property type="entry name" value="FERM_F0_F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13402">
        <property type="entry name" value="RGPR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13402:SF6">
        <property type="entry name" value="SECRETORY 16, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="312" mass="34065" checksum="A9915139E9994234" modified="2011-11-16" version="1" fragment="single">FDEIPGSQLHTPKPHPLTLNQFGGPSPAEFAASYQEPSNPKFGFDGFPIPGSPEYTTRANSVIGANPPPSGLFSSRLSQSQQSQLYQQYEVEDDTVKDYVPIAEDEDEDDEDVKLAKKKLQEKQAKLKKQQEQQQREHQRQQQQRSQRGKGEDGRWFNFLGKNNDGKPKPTRANLGEKENLFYYDEEHKRWLDKTKPVEEQLKAAETPPPPMMKRKAPNGPSPLGAPPATGPGPARPGNALASPHLVATSAANEDGAAPPKPQPKSQANTPSLATASLDNLLAMSAGGSSTGKKAKRGGARRARVNVMDLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP81</accession>
    <name>G3AP81_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">E set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_61717</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Rho GDI family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375928.1">
        <property type="nucleotide sequence ID" value="XM_007375866.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP81"/>
    <dbReference type="STRING" id="619300.G3AP81"/>
    <dbReference type="GeneID" id="18874019"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61717"/>
    <dbReference type="eggNOG" id="KOG3205">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1885699_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP81"/>
    <dbReference type="OMA" id="IANDWTT"/>
    <dbReference type="OrthoDB" id="1683373at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005094">
        <property type="term" value="F:Rho GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.30">
        <property type="entry name" value="Coagulation Factor XIII, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000406">
        <property type="entry name" value="Rho_GDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024792">
        <property type="entry name" value="RhoGDI_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10980:SF3">
        <property type="entry name" value="LD16419P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10980">
        <property type="entry name" value="RHO GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02115">
        <property type="entry name" value="Rho_GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="15313" checksum="2E8C88A3B21D93AC" modified="2011-11-16" version="1">MVLKRHPDFEIIKFVLKVEGQDPVDVPVKDVDSIHIKIPGGKKYIMEMHFVIMNRKLENFRYIQVVKKAGITVRTREVEIGSYEPGDEVYVKKFPEDDSPGGFFSRGHYGANSTYYAGDEMLVSNDWTLEIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3APA7</accession>
    <name>G3APA7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">N-acetylglucosamine-6-phosphate deacetylase</fullName>
            <ecNumber evidence="2 8">3.5.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_61750</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>N-acetyl-D-glucosamine 6-phosphate + H2O = D-glucosamine 6-phosphate + acetate</text>
            <dbReference type="Rhea" id="RHEA:22936"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="3.5.1.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="11">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the metallo-dependent hydrolases superfamily. NagA family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.25" evidence="2 8"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375954.1">
        <property type="nucleotide sequence ID" value="XM_007375892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APA7"/>
    <dbReference type="STRING" id="619300.G3APA7"/>
    <dbReference type="GeneID" id="18874033"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61750"/>
    <dbReference type="eggNOG" id="KOG3892">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032482_0_0_1"/>
    <dbReference type="InParanoid" id="G3APA7"/>
    <dbReference type="OMA" id="PCRKGAH"/>
    <dbReference type="OrthoDB" id="10264777at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008448">
        <property type="term" value="F:N-acetylglucosamine-6-phosphate deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006046">
        <property type="term" value="P:N-acetylglucosamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00854">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000113">
        <property type="entry name" value="N-acetylglucosamine-6-phosphate deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003764">
        <property type="entry name" value="GlcNAc_6-P_deAcase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-PHOSPHATE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113:SF14">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-PHOSPHATE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038994">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="12">
        <location>
            <begin position="54"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="237"/>
            <end position="238"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="330"/>
            <end position="332"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR038994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW32678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="424" mass="46740" checksum="DAD1F8603760F569" modified="2011-11-16" version="1">MTATYTRFTNCHLIDNGQLYEFTDLYYNNQTKKISHPPSNPQLVTQTIDLHEQILAPGYIDIQNNGIYGLNYSNLNGGYTEKDIQEFKNFYQDAMAKYLTTGVTATCPTVTSNFPQVYEKVLPFYKKSRLDNQTDSLGAHLEGPFINIKKKGCHPVETFVDAKDGESKLIEIYGGDASLLENVCIITGAPEIPGILDVIPSIVERNIIFSIGHTMSDYKTGCKAIDNGATMITHLYNAMPQPHHRDAGVVGLINSPVVGDDKTPYFGIICDGVHVDPAMANLAYRSNPNKCVLVTDAMHLMGLPDGTYKWDAQVIVKTGDRLYLENTDTLAGAATTLPQCVRNLIQWSKISLPQAVKTVTNNAAVSLGLQKERGFLNIGCDADFVVLDKAGYVQKIYKLGNEIKSSDVKLETKIARDEKISAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3APG2</accession>
    <name>G3APG2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cytochrome P450 52A3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_67265</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375955.1">
        <property type="nucleotide sequence ID" value="XM_007375893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APG2"/>
    <dbReference type="STRING" id="619300.G3APG2"/>
    <dbReference type="GeneID" id="18875150"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67265"/>
    <dbReference type="eggNOG" id="KOG0157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_27_0_1"/>
    <dbReference type="InParanoid" id="G3APG2"/>
    <dbReference type="OMA" id="FHQLARH"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11063">
        <property type="entry name" value="CYP52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002974">
        <property type="entry name" value="Cyt_P450_E_CYP52_ascomycetes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047146">
        <property type="entry name" value="Cyt_P450_E_CYP52_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002402">
        <property type="entry name" value="Cyt_P450_E_grp-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287:SF1">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00464">
        <property type="entry name" value="EP450II"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01239">
        <property type="entry name" value="EP450IICYP52"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="461"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602402-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="515" mass="59198" checksum="C2A65F85B8F88A50" modified="2011-11-16" version="1">MQPIDYYTVFIKFLTSWYGILASSILIYSLAIYIKRERFRRQHGTKEPPSFREGGLFGLYLAYTLLKYKSTGELLDYGVRIIEKYGWTMKAQLFGLTVLVTGEPENIKAMLATQFNDFGLGRNPHLKPLLGDGIFTLDSEGWKHSRQLLRPQFAREQVAHVQKLEPHIQILAKHIRKNKGTGFDIQELFFRLTMDTATEFLFGESVHSLYDESIGLTTSEEVAGFAHNFDTAQKYLGSRVYTQAAYFFVDGFEFRRSIKGVHKLAQIFVRKALTMTPEVLEEKSKDGYTFLYELVKYTKDPKVLQDQLLNILLAGRDTTAGTLSFCFFELARNPEIWQKLRSEIYQHFGEEYDASQITFESLKSCEYLKWVINETLRLYPSVSANFRVAAKDTTLPVGGGPNGSSPVFVPKGTTVAYCYYITHRDPKYYGKDAKEFRPERWESLDKLGWAYLPFNGGPRICLGQQFALTETAYVIVRLAQLFPTLNTKDFRPYPPRINCQLTARHQDGVFIELSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3APK1</accession>
    <name>G3APK1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Urea transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_61262</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375448.1">
        <property type="nucleotide sequence ID" value="XM_007375386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APK1"/>
    <dbReference type="GeneID" id="18873803"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61262"/>
    <dbReference type="eggNOG" id="ENOG502QU2F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023225_0_0_1"/>
    <dbReference type="InParanoid" id="G3APK1"/>
    <dbReference type="OMA" id="WWTAFEV"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050277">
        <property type="entry name" value="Sodium:Solute_Symporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48086:SF10">
        <property type="entry name" value="AGR155CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48086">
        <property type="entry name" value="SODIUM/PROLINE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="127"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="318"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="392" mass="42486" checksum="B54DD36C93311EAF" modified="2011-11-16" version="1">MFLFMVGELSAISGALETLTGVNALAAVIVECVVTTIYTFFGGFRISFITDNFQGACVLILLIICAAGMGSYIEIDPSKVGPSGLLKSNKLGWQLVYILFVAIVTNDCFLSGFWLRTFASKTDKDLWIGTSLAAFVTFVITTLVGVPGFLAVWSGDLTVGDDEGYNAFFILLAKMPRWLVAFVIIFCVVLSTCTFDSLQSAMVSTISNDVFRNKLQLHWTRCIVILVMVPIVVLAVKVADNILQIYLIADLVSAAIIPALFLGLSDSWFWFLRGFDIMCGGLGALLGVFIFGTVYYGSAREGGKLLLIWNGIYSSEDWGAFGAFVIAPFGGILITLVAAGVRIAGAYCYSKATGKPFTALERVVMEEDEVEEVSTTYGSLEDHDKKIATESP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3APT3</accession>
    <name>G3APT3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_61334</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the YIM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375530.1">
        <property type="nucleotide sequence ID" value="XM_007375468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APT3"/>
    <dbReference type="FunCoup" id="G3APT3">
        <property type="interactions" value="148"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APT3"/>
    <dbReference type="GeneID" id="18873846"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61334"/>
    <dbReference type="eggNOG" id="KOG1198">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_3_1"/>
    <dbReference type="InParanoid" id="G3APT3"/>
    <dbReference type="OMA" id="NETPLAM"/>
    <dbReference type="OrthoDB" id="3509362at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050700">
        <property type="entry name" value="YIM1/Zinc_Alcohol_DH_Fams"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11695">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11695:SF294">
        <property type="entry name" value="RETICULON-4-INTERACTING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="4">
        <location>
            <begin position="24"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="360" mass="39217" checksum="2CAD017A9553DE3B" modified="2011-11-16" version="1">MVFDSEILNYKAYAYSDANHPVELVDDSIKLVKSASGEGYTVPANNILVKIHYAALNPVDIKLHHLAFGITSLFNPKKGIGKDYCGEVVAVGSEANTTVKVGDFISGLYFPVHAKGTVSQYLMLDTTKKDERVFTTVASNLSLAESASWPSVLGTAMCMLKGLEFKDKKVLVLGGATSVGRSVIQLLRSGGAKEIVATCSPRSEKIVKELGAASTIDYHSNLLNPVLESVKMTGEFNYVIDCCGGDDLFSNISTILQKGGTYNTIVGDRSGSSVYIMLISIFMGMSRVIASKLGFLSYTYNYLFLDPGFNFPEEAKKLLENGDVKVDIDHIYKFEEFNEGLKKLESGNYSGKIVIQITDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3APX2</accession>
    <name>G3APX2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_50859</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375569.1">
        <property type="nucleotide sequence ID" value="XM_007375507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APX2"/>
    <dbReference type="STRING" id="619300.G3APX2"/>
    <dbReference type="GeneID" id="18871492"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50859"/>
    <dbReference type="eggNOG" id="ENOG502S25J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_392400_0_0_1"/>
    <dbReference type="InParanoid" id="G3APX2"/>
    <dbReference type="OMA" id="FQQMFNQ"/>
    <dbReference type="OrthoDB" id="4096963at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="457"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="470"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="494"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="599"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="643"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="694"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="703" mass="78136" checksum="EE1ADD8E53C84346" modified="2011-11-16" version="1">MSELKSDSPVPTEKDTIETDNDSITKVDTHEPLVPPRPPKTRPPKKEASADIPSPIEPDQSDKPIPSIPERPKSTSVRVATPEPGTIPIIPKRPESKTGASSSPIPVIPARPEGKSSVSSSPVPTIPSSRPVKQVVMAPEKKEEVNENDGSTTPNKIPEHHDVTDHLHSTLNTSDAIDFDGDTTPGNSNTKITFKDEVDDEAQLRSDTSSFDDDVETVKDKDVDNTEVVGETDEEDTREQEVFQQDEEPVEKPEVDSEDETEPIQEEQKEEPAVEEPKEAIEVDKKEEVTQVEKAEGQDKSKEVVEEQVKEESKTVEETEKHVEEVKEDIDPKQSEITATVKEPIAEVKETQEQKPVTPTIPSRPPKKSPELAKVALNEPKPSIPPRPSKNIESPKPQKAPPPKPKKLSSRIAAFQQQLFEQSSNDASNAPPPIARKLTSEKTGFMSGVGIPLPGMFNPLQLKKQQEAANEQEEEEEEDSSAVERKVEPVASARRAKGPRGKRLPKALSEPVNIETESRFKLETGELFSLSFTKKEEPKEEVNEEDKDRLEEEKDVVADEDIAVEESSKGNDNDINDVAGKPDLVDEEIDKEDSTSFSEIEKENEIEPVIEKEEKESDSTEYSKENNQVVDHDNVEIDEEPELHDTAEDSKTSDKEQEQESSVEEEEVISKRVYSEGEVVNAESGKVGESSKEIDEEDELKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ59</accession>
    <name>G3AQ59_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">3-oxoacyl-[acyl-carrier-protein] synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_61968</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.41"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9 11">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376184.1">
        <property type="nucleotide sequence ID" value="XM_007376122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ59"/>
    <dbReference type="FunCoup" id="G3AQ59">
        <property type="interactions" value="465"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ59"/>
    <dbReference type="GeneID" id="18874130"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61968"/>
    <dbReference type="eggNOG" id="KOG1394">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_69_2_1"/>
    <dbReference type="InParanoid" id="G3AQ59"/>
    <dbReference type="OMA" id="QIGHCLG"/>
    <dbReference type="OrthoDB" id="5334845at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000009">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier-protein] synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017568">
        <property type="entry name" value="3-oxoacyl-ACP_synth-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005589">
        <property type="entry name" value="PRK07314.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712:SF336">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="POLYKETIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000447">
        <property type="entry name" value="KAS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 9" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="12">
        <location>
            <begin position="2"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="active site" description="For beta-ketoacyl synthase activity" evidence="10">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000447-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="443" mass="46709" checksum="5102938715D35128" modified="2011-11-16" version="1">MSTRVVVTGMGIISPLGVGLSHNWSRLIAGQSGLISTTTLPDYNEGWDKVPAKVIGKVPSGSLAEGNWDAEEHFDRGEARRLALFSQYAIASTQQALVDAKLVKNGSTAVAVEDDKRIGVAVGSGIGSFHDAYDNSVAFAASGYKKVQPLFIPKLLTNMAAGNISIKYSLKGPLHSVATACATGLQAVGDAYNFIKMDYADVMVCGGAEASIHPLALAGFARARSVTTDFNDDPTSASRPFDKSRSGFVLGEGCGILILEKLDHALARGLTEKDIYAEVSGYGISGDAHHITAPREDGEGAYNAMSQALARSNGITPQEIDYVNAHATSTVIGDRAENNAMARLFPNSLAVSSTKSSIGHLLGAAGAVEAIYTINAIKTSEIPPTLNLDSAGGHKDDDLDQFKFDYVANKSKTMEVRNAMCNSFGFGGVNSSVIFSKYPQQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQH1</accession>
    <name>G3AQH1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein NSG2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_62099</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the INSIG family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376296.1">
        <property type="nucleotide sequence ID" value="XM_007376234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQH1"/>
    <dbReference type="FunCoup" id="G3AQH1">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQH1"/>
    <dbReference type="GeneID" id="18874184"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62099"/>
    <dbReference type="eggNOG" id="KOG4363">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088332_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQH1"/>
    <dbReference type="OMA" id="IEWSSFL"/>
    <dbReference type="OrthoDB" id="205546at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025929">
        <property type="entry name" value="INSIG_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15301">
        <property type="entry name" value="INSULIN-INDUCED GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15301:SF3">
        <property type="entry name" value="PROTEIN NSG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07281">
        <property type="entry name" value="INSIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="318" mass="35198" checksum="81CDBFFEAF1F6944" modified="2011-11-16" version="1">MSNLRKSAILNATPSMSATTTSESGGDSSARLQHSDSVLNLTKPSLFGLYDNDDFSVSKETEEKDMEIVTRAQENQKKKPTVPTIAPQNLIKLPLVVKLLILSGSAFLYNEITKHINYKHFSDNKLANIPVTISNIFVSGFLSKFRISTYIPIDSDVGKVLDQLLALTIQGAGMGLLHPIMDRILPTYFTRRVLSSNPYPTYSTTYSNLFNDLIRSCITFLGISYAIRKIEWSSFLQVSILWSLLNPGLWLLLDGTLSGFLASLLGSFVACAGIFVENFAFVVLYGANSEDSIALWLWIASFFFCGIIIFGKIGRGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQQ8</accession>
    <name>G3AQQ8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_140229</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376384.1">
        <property type="nucleotide sequence ID" value="XM_007376322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQQ8"/>
    <dbReference type="GeneID" id="18870281"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140229"/>
    <dbReference type="eggNOG" id="ENOG502SEBG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1982386_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQQ8"/>
    <dbReference type="OMA" id="NSEYQRE"/>
    <dbReference type="OrthoDB" id="66409at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="126" mass="14611" checksum="BE479C04FEA2DC1A" modified="2011-11-16" version="1">METRRGFFTKLGFFCTTAVTLFIIGKRHIARHKDERHRVETDARIDSEDNEFGILAKRPGFPTNNPTLDYTAGDRKSKFEGGGNAYASRRPGDRLSVYTWVKMKYFSDEEEESKYYTPSRSDKVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQW8</accession>
    <name>G3AQW8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">Mitochondrial import inner membrane translocase subunit TIM44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TIM44</name>
        <name evidence="15" type="ORF">SPAPADRAFT_61777</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the Tim44 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375975.1">
        <property type="nucleotide sequence ID" value="XM_007375913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQW8"/>
    <dbReference type="FunCoup" id="G3AQW8">
        <property type="interactions" value="1043"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQW8"/>
    <dbReference type="GeneID" id="18874042"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61777"/>
    <dbReference type="eggNOG" id="KOG2580">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020932_2_0_1"/>
    <dbReference type="InParanoid" id="G3AQW8"/>
    <dbReference type="OMA" id="NFQMEPF"/>
    <dbReference type="OrthoDB" id="10265990at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.240:FF:000002">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017303">
        <property type="entry name" value="Tim44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039544">
        <property type="entry name" value="Tim44-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007379">
        <property type="entry name" value="Tim44-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10721">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10721:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04280">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037871">
        <property type="entry name" value="TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00978">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9 13" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Tim44-like" evidence="14">
        <location>
            <begin position="270"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR037871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW31197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="431" mass="48288" checksum="329D94DA80C3D7A8" modified="2011-11-16" version="1">MLRSTIVKPNQYVAAASVAKFAVPTLAARSFHYSRINFNDRPSGESPMKVFFNTFKQEVQKSSELKENIKALQDESGRMAESEAFKRAKEAYAKAQLGTSAAGKVVLKAADVAGDVAVKAWDSPVGKGVRTTVRVTADVADKAFEPVRKTQVYKDVSEVIDDGSSTAYGGFLTKEQREALRQKELQDRIKKGWKGPVRENEEAGGELVATEHKASGPGLNEKWEKFKLKSPVGRGISYLQEKWEESENGLIALVRTIFEKVTGFFAETEQAKVIKKLRMMDPNFRLTDFQKTLTNYIVPEILDAYIKNEDAVLKQWLSEAPYNVWQANNKQFIQQGLFSDSKILDIRGIEIVTCKTIQPNETPVIVVSCRAQEIHLYRKAKTGEIAAGTEDSVQLSTYAMVLTRVPEEFENKTTEGWKIVEFARGASRPFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQW9</accession>
    <name>G3AQW9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kynurenine 3-monooxygenase</fullName>
            <ecNumber evidence="11">1.14.13.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Biosynthesis of nicotinic acid protein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Kynurenine 3-hydroxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">BNA4</name>
        <name evidence="13" type="ORF">SPAPADRAFT_61779</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-kynurenine + NADPH + O2 + H(+) = 3-hydroxy-L-kynurenine + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:20545"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58125"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the aromatic-ring hydroxylase family. KMO subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.9" evidence="11"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375976.1">
        <property type="nucleotide sequence ID" value="XM_007375914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQW9"/>
    <dbReference type="FunCoup" id="G3AQW9">
        <property type="interactions" value="783"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQW9"/>
    <dbReference type="GeneID" id="18874043"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61779"/>
    <dbReference type="eggNOG" id="KOG2614">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023210_0_1_1"/>
    <dbReference type="InParanoid" id="G3AQW9"/>
    <dbReference type="OMA" id="REFMFIA"/>
    <dbReference type="OrthoDB" id="10053569at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00328"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004502">
        <property type="term" value="F:kynurenine 3-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070189">
        <property type="term" value="P:kynurenine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006569">
        <property type="term" value="P:L-tryptophan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000129">
        <property type="entry name" value="Kynurenine 3-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01971">
        <property type="entry name" value="Kynurenine_monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027545">
        <property type="entry name" value="Kynurenine_monooxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46028">
        <property type="entry name" value="KYNURENINE 3-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46028:SF2">
        <property type="entry name" value="KYNURENINE 3-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0274">FAD</keyword>
    <keyword evidence="2 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 11" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8 11 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0521">NADP</keyword>
    <keyword evidence="7 11 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 11" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="12">
        <location>
            <begin position="31"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="493" mass="56272" checksum="EB1E0ECB45B4E058" modified="2011-11-16" version="1">MDSVNTSAILENNHHIPTMVNSHDSSSQGETVGIVGAGLVGCLAALAFAKKGYSVTLFELRSDPRTVKDRSQKNLRSINLAISNRGIRAMKYVDEEITDRVLEYIVPMKGRMIHDLTGTKQESQLYGLYGECINSIDRGFLNECLLNELDMQENVRVLFDHKLIRLNDITTEVKPSMVFLDSKQHNEFKTFKFDYIVGADGAHSQFRYQMSRFSRLNYSQNYVDMQYLELYIPPNEDPEAVSRFHINPNHLHIWPRHNYMLIALANKDGSFTSTFFSPWSVIESLKTSEEFLTFFQENFPDAYDLIGEEALATAYEKNPRGSLMQVSAYPYHSPNGRAILIGDAAHSMVPFYGQGMNCGFEDVRVLMELIEETEDIQAAFIKYSDVRKKDLDAICKLALENFYEMSTKVTSIGYLIKKNLDYTLAKYCNGKYFQWLPLYTMISFRDDIPYHEAVEIEARQQKTLTGINYAVAGAAIVGVVTKLLQLYSKLNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR12</accession>
    <name>G3AR12_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_62284</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376451.1">
        <property type="nucleotide sequence ID" value="XM_007376389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR12"/>
    <dbReference type="FunCoup" id="G3AR12">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR12"/>
    <dbReference type="MEROPS" id="C19.002"/>
    <dbReference type="GeneID" id="18874244"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62284"/>
    <dbReference type="eggNOG" id="KOG1867">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008279_6_1_1"/>
    <dbReference type="InParanoid" id="G3AR12"/>
    <dbReference type="OMA" id="HAREPIE"/>
    <dbReference type="OrthoDB" id="2020758at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02662">
        <property type="entry name" value="Peptidase_C19F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF888">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="1"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="297"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="410"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="412"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="486" mass="55062" checksum="69C97E4765C0E4B9" modified="2011-11-16" version="1">MPNGPKQNFFSGYNQEDAQEFYQLVMNLLEREYKNVSTSRMPTPEPESGPTEKFIDMNHVKEIVSGVDQLGKLGVVYVPASQVDPNLVDCDHKLFPLELITPVDGITAERIGCLTCGEVGGIRYSVNSGLSLNLPHDASYYSSFNLNDLLNQWITPEIIEDVNCNRCGLIQTRDFLKNRLEDLSQGDTSSKLIEQFQTRLSLIEQELDQHHITDEIFEKLTIKKQIKKSKKSKQIYLSRPPPLLCIHINRSVFDPRTYSIVKNPSNVDFPIKLNMTPYVVTPNDINMDARLPFRKQDQHAREPIESVPDVSSTPSSSSLSSDEVPSTSTAATSANIEDTPVDPKLLYNLKSVISHYGTHNYGHYICYRKLRGSWWRISDESVYVVSEEEVLNGQGTFLLFYEFDDGYKEQLEDVSSEEEEEEEKGIKLMEQHASSESSDLEDEDYSDSVKESSSSSMHESKNFDDDDESMNSSHIDLAVAEAQVHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARD1</accession>
    <name>G3ARD1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_62336</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376517.1">
        <property type="nucleotide sequence ID" value="XM_007376455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARD1"/>
    <dbReference type="FunCoup" id="G3ARD1">
        <property type="interactions" value="1156"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARD1"/>
    <dbReference type="GeneID" id="18874277"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62336"/>
    <dbReference type="eggNOG" id="KOG1691">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066963_3_0_1"/>
    <dbReference type="InParanoid" id="G3ARD1"/>
    <dbReference type="OMA" id="DVFEACF"/>
    <dbReference type="OrthoDB" id="4084947at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442677" description="GOLD domain-containing protein" evidence="9">
        <location>
            <begin position="19"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="10">
        <location>
            <begin position="31"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW31739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="214" mass="24629" checksum="1334FCD423A861DD" modified="2011-11-16" version="1">MKSLSILIISFLCMLINALHMEIPSSPNPSPVCIRDFVQKDQMVVINLKTDGNKGDGQRLDLKVTDSVGNEYRNKRDILGEVKIAFTSHDNAAFDICFTNHQEKKWGRNSKISFIRNVELDIEIGAAARDWNALQASEKLKPIELELRKIEGLTSEIVEELQYLKAREERMRDTNESTNSRVKWFSITVILALIGLGVWQIQYLRHYFKVKHII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARD9</accession>
    <name>G3ARD9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_62340</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376524.1">
        <property type="nucleotide sequence ID" value="XM_007376462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARD9"/>
    <dbReference type="GeneID" id="18874280"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62340"/>
    <dbReference type="HOGENOM" id="CLU_783396_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARD9"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442563" description="Hyphally-regulated cell wall protein N-terminal domain-containing protein" evidence="7">
        <location>
            <begin position="19"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="8">
        <location>
            <begin position="27"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="274"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="306"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="354" mass="38624" checksum="CBAD2F93FD280410" modified="2011-11-16" version="1">MQLLSTIIICLLAQTVATEETLEVSATSTIETSTISEPVVVESDGVLNLLNMEELNASNFHVDNSGQFLFKSNVRSEVDIGYLNNSGYILLDTIEADRAYLHLGNNTENNGELVVYGQHLNVHMDSVENSGSITVWGYAGEVTMEQFTNKKDVCFHEQLVELKQSKGAGCFAVSNSSVYISNPDEFHPTIVLEDSQNHVYLEEISEDLVINLVNFQNVVRVTAPNDGYENFSYTNGSLILAYEDFRITLEIGSGYDSERISVENDDSGVLVSYDGSAPSKTSHETECGCALETDPPVKEAESSTVEAEDESSSTNDDDNGKGNGNQNSRNSSASVLTPIYLVGLSFVVSLMIML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARI2</accession>
    <name>G3ARI2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_56179</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376081.1">
        <property type="nucleotide sequence ID" value="XM_007376019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARI2"/>
    <dbReference type="FunCoup" id="G3ARI2">
        <property type="interactions" value="92"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARI2"/>
    <dbReference type="GeneID" id="18872011"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56179"/>
    <dbReference type="eggNOG" id="KOG2079">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000917_0_1_1"/>
    <dbReference type="InParanoid" id="G3ARI2"/>
    <dbReference type="OMA" id="NQLFFHQ"/>
    <dbReference type="OrthoDB" id="289913at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059070">
        <property type="entry name" value="TPR_VPS8_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025941">
        <property type="entry name" value="Vps8_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 8 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23410">
        <property type="entry name" value="Beta-prop_VPS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12816">
        <property type="entry name" value="TPR_Vps8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25066">
        <property type="entry name" value="TPR_VPS8_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23413">
        <property type="entry name" value="zf_RING_Vps8_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 8 central" evidence="3">
        <location>
            <begin position="558"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="VPS8-like TPR-like repeats" evidence="4">
        <location>
            <begin position="1093"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW31303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1250" mass="143839" checksum="0F510089689856A1" modified="2011-11-16" version="1">MTSQSSANSSSNSLLSDTTKSITSPLRKQRFDDRSLTKTSLALMSYQSMTSLQSPSPIADNNQSDQITNIFKWSEMNHLSQLLPSDSPSHLLEATSIYIALSTPQGIAIFTYDQKLECMLTTHESIPASNITCMTFSSDSTFLAAGFTSGHIILWDMNGDRIPSNSHQTISPYYTINPITLSSRFTKNIHGHIINTKVTSLSFISNSISRLISVDDSGLVFYHNGVKKFMNVHFTCQKILGRNDANISDEKFKILQCEMLPWGNSQQITDQMGVVALMTHTLVLIISTSSLNSKIPKVKQHFKIGKSNLVGDSPELHTTLSWFPCMEVDGKVNNARLAYSYNNVLTILELDNSSFPDNFLKVVNDLKDKDKAIQQLPFRKQCRWINQGVVSFMKWVSKDILCVVTANTLVSLYYNGKSLKPVSVDNLDFTIRNMIQFKNRLLVSTSLIKLFMGKPLTWADILLEKLAKGDYHQALTLANNYYNSTNNGKLVLVGLPKDQTQRALLIQPYLERIMKESLPYLFKEEDHSYLTVYFNIIAYIGGYDILENLYEVVPDAEFFNTLEPFIQSGSIISLPPLVLKRLVEHYVAIEKGDILTELICTLDIRTLDIDLTISLCKQFELRDCLVYVWNFILHDYETPLIEFILDFSSLSRDDQLSAYTYMSYILTGRQYPTDRFIPIESATRAKQSICDILFSSTLVTRQDNLIETDNDSSFPYLYTFLKANSFEMLSTMNEFFEDPYLNESTLTRQYLTEALLDVYEMNDELTTWDNIQLAIFIGRNYPKYSQFLRLSESTITDMFDVLCSADDVSIRQDCELALASLLPFVPDDGLIIDKIRVAKYFTVLINVYKSKGEYSKALEVWLDKEHDDDNESVAQLIENGFMLSKNAHDRVNLIRLLRQEFVRFVAMDLIMFCNVVEIYCPSVHNEVLNIKDDALAYNYLKVLFEKRSDLEYEYVVRYLELICLFNRDHVFEYVNEYKKTMNDHREQCMEILKDHVDGQALLMVSRGEYTQAEEILLQQMAKITPSLESDESVRSEFNHLLQYIIKICDDAHDNEELWLELLHQLVDMSNSESTYIHDFINTCIHDCFRYISDNSKSKQQQLVIFNRFLEQYSSTDKIATLSNIRGVLQQVFISYSYESEMLNISLKMINGDIYKNSQIIKANKLKGWNIICKNCSSCGKVMWGSGGYNLDDHILAWEARQWSKAHDIELEDKEKFHHCELIFFKCEHGYHMKCLENLGANDEKYCVICE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARP2</accession>
    <name>G3ARP2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-dehydropantolactone reductase</fullName>
            <ecNumber evidence="4">1.1.1.358</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">2-dehydropantolactone reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Ketopantoyl-lactone reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_62388</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(R)-pantolactone + NADP(+) = 2-dehydropantolactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16719"/>
            <dbReference type="ChEBI" id="CHEBI:18395"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.358"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>isatin + NADPH + H(+) = 3-hydroxyindolin-2-one + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:68608"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27539"/>
            <dbReference type="ChEBI" id="CHEBI:28536"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="1.1.1.358" evidence="4"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376573.1">
        <property type="nucleotide sequence ID" value="XM_007376511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARP2"/>
    <dbReference type="FunCoup" id="G3ARP2">
        <property type="interactions" value="131"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARP2"/>
    <dbReference type="GeneID" id="18874302"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62388"/>
    <dbReference type="eggNOG" id="KOG1577">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_0_1_1"/>
    <dbReference type="InParanoid" id="G3ARP2"/>
    <dbReference type="OMA" id="PWCMRQE"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047011">
        <property type="term" value="F:2-dehydropantolactone reductase (A-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004032">
        <property type="term" value="F:aldose reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019568">
        <property type="term" value="P:arabinose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042843">
        <property type="term" value="P:D-xylose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042180">
        <property type="term" value="P:ketone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19071">
        <property type="entry name" value="AKR_AKR1-5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF13">
        <property type="entry name" value="ALDO_KETO REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="10">
        <location>
            <begin position="27"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="9">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00050878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00051098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00066965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00079693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00081322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW31795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="245" mass="28185" checksum="6BAD365DD4929A3C" modified="2011-11-16" version="1" fragment="single">MSYRLIELNSGKKIPSIGLGCYNIPISQTSNIVYEACKVGYRHFDTAVLYGNEEQVIEGISRYLRDHPDAKRSDFFYTTKLWNSQLGHQPTIDAINIMKSQIGDLQYIDLLLIHSPLPGKTKRLESYKVMQEYVDKGLLKNIGVSNYGKHHIEELLNWSGLKYPPVVNQIEISPWVMRTDLAQWCLKQGIQVEAFSPLTHGQRLRSDVFSDIRKKYDKSLAQILIKWSLQKGYIPLPKTKTVSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARQ6</accession>
    <name>G3ARQ6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NADH kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_62402</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376587.1">
        <property type="nucleotide sequence ID" value="XM_007376525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARQ6"/>
    <dbReference type="FunCoup" id="G3ARQ6">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARQ6"/>
    <dbReference type="GeneID" id="18874312"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62402"/>
    <dbReference type="eggNOG" id="KOG2178">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008831_10_2_1"/>
    <dbReference type="InParanoid" id="G3ARQ6"/>
    <dbReference type="OMA" id="IPKYQES"/>
    <dbReference type="OrthoDB" id="24581at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042736">
        <property type="term" value="F:NADH kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.30:FF:000014">
        <property type="entry name" value="Mitochondrial NADH kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF26">
        <property type="entry name" value="NADH KINASE POS5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW31809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="419" mass="46778" checksum="63D7C785858B750F" modified="2011-11-16" version="1">MLRAFLPRQPLLLGPTQNLVRCLSMSHSAVSSTLSNSSSNNNSNRPLVLVKSCGDLPQAKLPEYIKSTQGRLYHIIWRSAPPQNVFLVKKPWDTTVREAMIELINHLHQQYPAVNVIVSEDVADELVHETSTITKLFDPSIRHIIYTGTKSEIVDKTDLMVTLGGDGTILRGVSIFSNSIVPPVLSFAMGTLGFLLPFDFKHCQDTFKMVYENRSKALHRNRLECHVHREHCVTPDCENEEPIEMIHAMNDISLHRGNLPNLTAVDIFIDDEFFTTTVADGLVFATPTGSTAYSLSAGGSITHPLVPCILLTPICPRSLSFRPLILPNSSDIKIRLSKNNNRNQSIELTIDGIPQPDLRPGDELHVRSEEQIKCDHDKQQPVNGIMCIAKGKNDWTKDINELLGFNSGFKSQTNRKSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARW4</accession>
    <name>G3ARW4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription and mRNA export factor SUS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SUS1</name>
        <name evidence="3" type="ORF">SPAPADRAFT_56573</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in mRNA export coupled transcription activation by association with both the TREX-2 and the SAGA complexes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction and promoter selectivity, interaction with transcription activators, and chromatin modification through histone acetylation and deubiquitination. Within the SAGA complex, participates to a subcomplex required for deubiquitination of H2B and for the maintenance of steady-state H3 methylation levels. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery. May also be involved in cytoplasmic mRNA decay by interaction with components of P-bodies.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the nuclear pore complex (NPC)-associated TREX-2 complex (transcription and export complex 2), composed of at least SUS1, SAC3, THP1, SEM1, and CDC31. TREX-2 contains 2 SUS1 chains. The TREX-2 complex interacts with the nucleoporin NUP1. Component of the 1.8 MDa SAGA transcription coactivator-HAT complex. SAGA is built of 5 distinct domains with specialized functions. Within the SAGA complex, SUS1, SGF11, SGF73 and UBP8 form an additional subcomplex of SAGA called the DUB module (deubiquitination module). Interacts directly with THP1, SAC3, SGF11, and with the RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ENY2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376591.1">
        <property type="nucleotide sequence ID" value="XM_007376529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARW4"/>
    <dbReference type="FunCoup" id="G3ARW4">
        <property type="interactions" value="337"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARW4"/>
    <dbReference type="GeneID" id="18872048"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56573"/>
    <dbReference type="eggNOG" id="ENOG502S9WJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134052_2_1_1"/>
    <dbReference type="InParanoid" id="G3ARW4"/>
    <dbReference type="OMA" id="YESGWFD"/>
    <dbReference type="OrthoDB" id="6221744at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071819">
        <property type="term" value="C:DUBm complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03046">
        <property type="entry name" value="ENY2_Sus1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018783">
        <property type="entry name" value="TF_ENY2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038212">
        <property type="entry name" value="TF_EnY2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12514">
        <property type="entry name" value="ENHANCER OF YELLOW 2 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10163">
        <property type="entry name" value="EnY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 2" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="95" mass="11021" checksum="C1307775387DB6FC" modified="2011-11-16" version="1">MNQSELDTIKSKIQDHLITSGHYDTINKQLKLQLYESGWFDKVSQIANTELKDNPQINFDSLYSFVKPKATELVPGEVKHEVLERIKAYLDEIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARX8</accession>
    <name>G3ARX8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Polyubiquitin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">UBI4</name>
        <name evidence="9" type="ORF">SPAPADRAFT_51800</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376605.1">
        <property type="nucleotide sequence ID" value="XM_007376543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARX8"/>
    <dbReference type="SMR" id="G3ARX8"/>
    <dbReference type="FunCoup" id="G3ARX8">
        <property type="interactions" value="664"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARX8"/>
    <dbReference type="GeneID" id="18871596"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51800"/>
    <dbReference type="eggNOG" id="KOG0001">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010412_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARX8"/>
    <dbReference type="OMA" id="MSDEHTL"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000004">
        <property type="entry name" value="Polyubiquitin Ubiquitin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="77"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="153"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="229" mass="25762" checksum="E428C09C461F7F05" modified="2011-11-16" version="1">MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARY1</accession>
    <name>G3ARY1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_62445</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376608.1">
        <property type="nucleotide sequence ID" value="XM_007376546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARY1"/>
    <dbReference type="FunCoup" id="G3ARY1">
        <property type="interactions" value="947"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARY1"/>
    <dbReference type="GeneID" id="18874319"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62445"/>
    <dbReference type="eggNOG" id="KOG4210">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027451_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARY1"/>
    <dbReference type="OMA" id="RVWVNQL"/>
    <dbReference type="OrthoDB" id="1875751at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12397">
        <property type="entry name" value="RRM2_Nop13p_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034226">
        <property type="entry name" value="Nop13/Rnp24_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF95">
        <property type="entry name" value="NUCLEOLAR PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="117"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="227"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="322"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="327"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW31830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="393" mass="44461" checksum="6D82E1CFD3A81137" modified="2011-11-16" version="1">MSNSSSEDIPVEVQPVENKKESKKEKKLRKQEKKNKKSKKTDEVEQSEEEQEDEQEDDEQEANDDEIEIDLTASVPLSKKQQRLLKKGKLDLDKLAKKHPIPKAADAEPEKPSKSEFGVWIGNLSFDTSREDIIRFIVGKTSQLEDESLPKVVEEDITRVNIPKKDGNKIKGFAYVDVPSLAHVNSIVALSESVLNGRKLLIKNANSFEGRPAASPTSALSKNPPSRILFVGNLSFDTTEDNLQEHFRHCGDIVRIRMATFEDTGKCKGFAFIDFKDEEGPTTALKSKLAKMLINRKLRLEYGEDRSKRNPHMRARANDEVIEGEVPEEKHVPVERVERRPRTEEPAPRKRVFKERRQPDSNNKRQKSSVALATAQRASAAIVPSTGKKITFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS23</accession>
    <name>G3AS23_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TATA element modulatory factor 1 TATA binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_155618</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376650.1">
        <property type="nucleotide sequence ID" value="XM_007376588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS23"/>
    <dbReference type="FunCoup" id="G3AS23">
        <property type="interactions" value="515"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AS23"/>
    <dbReference type="GeneID" id="18871024"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_155618"/>
    <dbReference type="eggNOG" id="KOG4673">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019193_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS23"/>
    <dbReference type="OMA" id="NWESIEF"/>
    <dbReference type="OrthoDB" id="74178at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052602">
        <property type="entry name" value="Growth_transcription_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022092">
        <property type="entry name" value="TMF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022091">
        <property type="entry name" value="TMF_TATA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46515:SF1">
        <property type="entry name" value="TATA ELEMENT MODULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46515">
        <property type="entry name" value="TATA ELEMENT MODULATORY FACTOR TMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12329">
        <property type="entry name" value="TMF_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12325">
        <property type="entry name" value="TMF_TATA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TATA element modulatory factor 1 TATA binding" evidence="6">
        <location>
            <begin position="680"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="33"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="253"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="376"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="486"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="691"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="133"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="202"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="799" mass="91056" checksum="5A3351B20D80A476" modified="2011-11-16" version="1">MEIKPSSPTHLASPDEVEDLISLNEPIAEVIAQQNETKDESQQLDSDEVNNDPAVTAPSDEITQEEKITQQDQSSSEVGAVLQPVKQEPIPDQSQKTETEQDTEQNLQSENAPDVKVQVDSIDQTSSALGPEATEKEPEQNKESSDPAQESEKKEPEPQTETQTETKAESEPVPKKPVRKRLTLQERLAQAAKGKSKKSTKSKSPSAEPPASTTATPAPQPVVDTVIPEEAPVEIASPSAVSLTTVNPFEKEREELLRKISAQESMIKELREEGESLSHKELKLNDTIKKLKNTNLQLEENLQDYLIKSDESSLKLQEFQEFLRLHKFKSFDQFKEKFGELNYKLANTTQELEQANGLQQKYSDLVLTHEQEVSLNKATVKELNDLKIQIDIMKSQHSLELDSKENILQDLKNELMSAKQSYGREISRLEDKIENLRLEQESNVTISETSDESDKGKIEEFIKLSESHHNLQKQYLSSQQNWKVIESNLLLKIDNLTNTIEQLKKQKNKLTQDLTKVNNLLISSDNEVRKLTQEVTTLEQQIQNHELSIKIKENDFVELQDKFDKLQHIYNQDRAKLNTKIETLNDTIEKLKEQQKSASELQLNLDNSTRQISLGNLSAASWNDIRFGESSTTPAINRDFSGIYIDNSRNQSSTSFTEIGVEDGYDLRGESVYNHLGNIPTSSHSNNIQLVNKMSSTIRRLEIELNTLKDEYSKLSMDKEKLEQELFESLKLNDEMSQMNVQIDELKKQISEKDRKEQTMLELIGEKSEQVEELKADVFDLKELCKLQVQQMIEIQQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS98</accession>
    <name>G3AS98_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CIP1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_62497</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376671.1">
        <property type="nucleotide sequence ID" value="XM_007376609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS98"/>
    <dbReference type="GeneID" id="18874349"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62497"/>
    <dbReference type="eggNOG" id="ENOG502S12R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058266_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS98"/>
    <dbReference type="OMA" id="QPEWEGK"/>
    <dbReference type="OrthoDB" id="9974981at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051609">
        <property type="entry name" value="NmrA/Isoflavone_reductase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47706:SF9">
        <property type="entry name" value="NMRA-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47706">
        <property type="entry name" value="NMRA-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="292" mass="32456" checksum="912D7735669F3302" modified="2011-11-16" version="1">MSPVSIAIIGLNGSLGKPTLEAINSGKFDSKIKFPIKALTRKAQTSTDKIQYIQTEITPDTIDSIVEKLRGTDVIIELVSHNPDLLNTIEPIIVKSQPKLFIPSQFGSDLATMNKYIPGFLGFKNAHSENVRKAGIKSVDIVTNFFAEPSKFLYEVVGHVGIDPANKSILQLGDLNTRVAFTRVEDIGNVIVAVATTAPESLPDKIRVKSGSISYQDAIDRYEQTHNVKLEVKEKYTVEEAKQQLSEKLAKGFNPADFLWYLHVGGATGDLNYDDDNELINPNQFLWKWTEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASH3</accession>
    <name>G3ASH3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methionine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="2">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Methionyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_156764</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="2"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377124.1">
        <property type="nucleotide sequence ID" value="XM_007377062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASH3"/>
    <dbReference type="FunCoup" id="G3ASH3">
        <property type="interactions" value="566"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASH3"/>
    <dbReference type="GeneID" id="18871057"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_156764"/>
    <dbReference type="eggNOG" id="KOG0436">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009710_9_0_1"/>
    <dbReference type="InParanoid" id="G3ASH3"/>
    <dbReference type="OMA" id="MDTQAFC"/>
    <dbReference type="OrthoDB" id="24670at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.220.10:FF:000002">
        <property type="entry name" value="Methionine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023457">
        <property type="entry name" value="Met-tRNA_synth_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43326:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43326">
        <property type="entry name" value="METHIONYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methionyl/Leucyl tRNA synthetase" evidence="11">
        <location>
            <begin position="28"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="587" mass="67898" checksum="3869226F83D386FE" modified="2011-11-16" version="1">MATRYNNMIIRSRPWYIFKRLNSTKPFFTSTPIFYVNAAPHIGHLYSMLLADVRTRWEKLDPNKKAYFLTGTDEHGLKIQAAAEKQGIDPKLFVDQVSTNFKDLANLVNIDYDRFIRTTDADHITLVRHFWEMMMEKGYIYQGSHKGWYCVSDETFYPDNQIEEFVNKEGETKKRSIETKNEVVYQEENNYFFKLSEFQSQLLTFLKSNPDFIKPKSKYELIVNELTKNKLPDLSISRPASRLKWSIEVPNDPTQKMYVWFDALLNYLTAAGFPQGFTLGEQGQYITSPTNIWPATHVIGKDIVRFHCIYWPIFLMAAKIDLPRQVIVHSHWLVDGVKMSKSLGNVIDPVDISKYYGIDPVRFFLIENSNIADDCKFSEVALSSTRDMLLGKYGNLFMRIGAKNFNVRESVQDYHSGKFTNIEDTIKAHTINRDSVDILIQQRQELIEKLDSLYERMNENFVNFDSMKAMQSWWSVLDATNGIFQTAEPWLYAKKIKQDDPSKQEYANLISSYVFMCAETCRITSILLQPIAPELAERMLDRLGVAKDNRNSTFAKFGADSSYGAGANDKGHKVPLEKVELRIEAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASI3</accession>
    <name>G3ASI3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA polymerase delta small subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_63028</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase delta/II small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377134.1">
        <property type="nucleotide sequence ID" value="XM_007377072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASI3"/>
    <dbReference type="FunCoup" id="G3ASI3">
        <property type="interactions" value="887"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASI3"/>
    <dbReference type="GeneID" id="18874560"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63028"/>
    <dbReference type="eggNOG" id="KOG2732">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021763_1_0_1"/>
    <dbReference type="InParanoid" id="G3ASI3"/>
    <dbReference type="OMA" id="HCILIGT"/>
    <dbReference type="OrthoDB" id="3763at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016035">
        <property type="term" value="C:zeta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006278">
        <property type="term" value="P:RNA-templated DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040663">
        <property type="entry name" value="DNA_pol_D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024826">
        <property type="entry name" value="DNA_pol_delta/II_ssu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416:SF0">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18018">
        <property type="entry name" value="DNA_pol_D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase delta subunit OB-fold" evidence="4">
        <location>
            <begin position="39"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="3">
        <location>
            <begin position="199"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW31101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="427" mass="47220" checksum="F0EC53F4B7609EFF" modified="2011-11-16" version="1" fragment="single">MSQYINQNLTNPLLIRPTSKINPGEEFLLPPQGRNYNHQYYTMYQHRFSALKSRTEAKALAKWGNGQHDIDGQTIFKQDKILDIVSGKLCWVIGTVYIDAPGKLNILKDVENGVDDVLPGVPEAYVSEENTGVVMLEDESGRAILHGSEFLDKNLLVTGCVVGVLGIEVQAGVFEIMDVVYPASAPQSKRAVDNEPSKIVLVSGLNLSPIGDLKTELLKQYLTGELGSDEDKHVNSNVSQVIIAGDSILPLKQEQNQDFMSTNNYGTKNISKYNTDSLKKLGEFINDVIVSLPVAVMPGSNDPGEICLPQQPLHRSLLGNFSYGDRLTRLTNPQWISIDGVKVLGTSGQNVDDIKKYAKRSEVTDIMKASIKWQNLAPTAPDTLYCYPYDNADPFILTDEVPHVYFVGNQEKYASEVFQYNGSVTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASI4</accession>
    <name>G3ASI4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_141608</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377135.1">
        <property type="nucleotide sequence ID" value="XM_007377073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASI4"/>
    <dbReference type="GeneID" id="18870385"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141608"/>
    <dbReference type="eggNOG" id="KOG0134">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012153_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASI4"/>
    <dbReference type="OMA" id="QIWGQGR"/>
    <dbReference type="OrthoDB" id="276546at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02933">
        <property type="entry name" value="OYE_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000337">
        <property type="entry name" value="NADPH dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF91">
        <property type="entry name" value="NADPH DEHYDROGENASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="4">
        <location>
            <begin position="10"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW31102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="397" mass="44594" checksum="997ABE7079B20371" modified="2011-11-16" version="1">MTLSKFQSSNLFKPIKIGQTTVRHRIAQLPASRNRASGHVPTDLMKQYYTDRARHAGLISTEATLISLEQGIYPMIPGIFNERQMNAWKEIVDGVHEQGAVFALELQALGRVGDAKLLKEAGLPLTGPSAIYENEEKGKVAIEAGNPIQELTEADIKDIIYRQYTNAVKLAMEAGFDFVELHGGYGYLLEQFIHPGVNKRTDKYGGSIENRARFLLELVDHLSTIIDPAKLAVRFTPVNFFQVPGANPTSQEDYSYIVSELQKRADEGKKLAYISIVDGTFADATMDKTVALDFGYIHKIWKGIIIRGGNYHNDQENWAVLERDASADDRTLVGLCRHYVANPDLPERIKKGLPLNECDKPNFYTPSNWGYNTYPFYGQDVTFDEEVEKKKFGIPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASQ6</accession>
    <name>G3ASQ6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_52292</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377153.1">
        <property type="nucleotide sequence ID" value="XM_007377091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASQ6"/>
    <dbReference type="STRING" id="619300.G3ASQ6"/>
    <dbReference type="GeneID" id="18871656"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52292"/>
    <dbReference type="eggNOG" id="ENOG502RQGH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_334381_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASQ6"/>
    <dbReference type="OMA" id="ITHIQIN"/>
    <dbReference type="OrthoDB" id="4024489at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF210">
        <property type="entry name" value="CHAOPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="854" mass="97946" checksum="866A3EFD987C0D86" modified="2011-11-16" version="1">MSDPILANLDTLPPEIIQLIVDYIPLTSQRSLVDFQLFRKFVLPKLLSDIHVLQGGLSVIRDPFKEYFNPDDPYKLGLKEYTPICGYQEYETIKSEPLYLANSISLIKIVEKYGVFPQSISFEDHLSFFEILQSFPEIIANAKQIYIRMMNLNPISRTSNSWRSILNSVSALPLNITTLELISVPIDNIQFSSTLNELIYQGKIRDCKRVFEWLTNLTRLTLRGVQIQQIDLSSLPDNIEYLNIADLKITQPNLQLPKSLKELIVSTATAELSDLYTSFSLKFPPLEEFRRFEFSCDELTNLTTISLPMCVEELVLRKCRNLFLVESLEKYVNLRTFEWLGALLSPSFYLLSPIPPNLERLIIDGDNAKTSMVINILARAMPSYFYSIYGMYTFRVGTEFKLPESLKELSIRNCQFLEINCESLALPTNLKSITLENLAGFVGYLQELALPSSITYLNFSSMKIDFIDRVVFPASLRKLYLDDNKLRRVSETANLRQLQHLQKVSLTGNSLESNYTVSINLPKTITHIQINDTPVLMEMPFDNMKQLTVPVSVTKSSYLSLNANLEVLVIKNNFEFNEDFRLPANLRILILDEVPNLKISKSEWLENLPENLQEIRISVSKRGSCEISPLLQTVKFPSSLRLLTLQNLNISSETYSKFDFSECTKLSSMQIDNGNVSEIDLNCLPEFLTDLKLQNLKIHNFIGNFARFINLKSLFLSGNKLRKYVTCNNLILPPSIEDLSLMQCNIKTIEISNIQECKVMRQVNLSKNSWMDPVKVANVARELNKVSHEFSCVLVDDVIMDDDLILMQRNSKLTFNFQAEGESLKDYSLIYSCPTGIRCREEEYEETDNSEDEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASQ9</accession>
    <name>G3ASQ9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_142548</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377156.1">
        <property type="nucleotide sequence ID" value="XM_007377094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASQ9"/>
    <dbReference type="GeneID" id="18870460"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142548"/>
    <dbReference type="eggNOG" id="ENOG502RA02">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156003_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASQ9"/>
    <dbReference type="OMA" id="CQVIKYI"/>
    <dbReference type="OrthoDB" id="4022411at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990879">
        <property type="term" value="C:CST complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016233">
        <property type="term" value="P:telomere capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024222">
        <property type="entry name" value="Ten1_fungal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12658">
        <property type="entry name" value="Ten1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="132" mass="14532" checksum="0B90B57E7A3C1119" modified="2011-11-16" version="1">MTRLILEPGSLSSLYPDASVSQNYRVRIIAQVLEYHPDNATLTLIQVPTLNSSDSSTITINIFEQLGDISGDVIRKGTVVNLEGFYDGNTVIVIDIYPINGAHLNVENLETIQSLNNLPSFYHDSGSRPNAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT52</accession>
    <name>G3AT52_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_52013</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376849.1">
        <property type="nucleotide sequence ID" value="XM_007376787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT52"/>
    <dbReference type="FunCoup" id="G3AT52">
        <property type="interactions" value="367"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AT52"/>
    <dbReference type="GeneID" id="18871618"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52013"/>
    <dbReference type="eggNOG" id="ENOG502QVC2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037639_2_0_1"/>
    <dbReference type="InParanoid" id="G3AT52"/>
    <dbReference type="OMA" id="NIQRRMD"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12400">
        <property type="entry name" value="RRM_Nop6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000573">
        <property type="entry name" value="Nop6p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034228">
        <property type="entry name" value="Nop6_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF51">
        <property type="entry name" value="NUCLEOLAR PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="79"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="154"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="57"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="208" mass="24071" checksum="7D323709109C1F74" modified="2011-11-16" version="1">MSEEKLSKKQKKALGFRKTKEEREEIKEKKRKAETEKEEEEVKEPTTDANTEDQPPVKKRKTRRGKKGKGVNGGAGPRFIIFVGNLPYDATPTELTAHFKNSSPDRIRIRPDKGIAFLEFDNDTKEIQSKMELALRMHHTLLRNRKINVELTVGGGGNSEFRRNKLKEKNEKMLEDRRKNDDLKKKKKETTKEPPQGIHPSRLAQLQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT72</accession>
    <name>G3AT72_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62703</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376868.1">
        <property type="nucleotide sequence ID" value="XM_007376806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT72"/>
    <dbReference type="STRING" id="619300.G3AT72"/>
    <dbReference type="GeneID" id="18874438"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62703"/>
    <dbReference type="eggNOG" id="ENOG502RQ0U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1016227_0_0_1"/>
    <dbReference type="InParanoid" id="G3AT72"/>
    <dbReference type="OMA" id="VQENYIR"/>
    <dbReference type="OrthoDB" id="4026410at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="31868" checksum="ACEDF677454DF7BD" modified="2011-11-16" version="1">MVTPTLLARSSAIIAPIVRNGIPLAYFARSTSLLPRQPLTRSFASTTVSFQPVTAPKLRAEIKAEKVLKKKLREKEAKKKQKEKELKAKELEKQKKQRELVKKKKEQEKEKKELAKYKKLEKKACSGNSSRFSLINFLLKYIPGSTLKDKHDAIKQLSEAEIEVYQKGCDKLVKEAFSHFKPKPDYPIIGYTKYVKKNYGTLPGPAHERMKHLAYSWRNLTKEEKSLYEADPEILKKHREDLKQWKTQRVSDYLVAKQIIKDFKFDPKDPSTYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT78</accession>
    <name>G3AT78_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_62710</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376875.1">
        <property type="nucleotide sequence ID" value="XM_007376813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT78"/>
    <dbReference type="FunCoup" id="G3AT78">
        <property type="interactions" value="162"/>
    </dbReference>
    <dbReference type="GeneID" id="18874442"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62710"/>
    <dbReference type="eggNOG" id="KOG2504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_1_0_1"/>
    <dbReference type="InParanoid" id="G3AT78"/>
    <dbReference type="OMA" id="DLVWQIS"/>
    <dbReference type="OrthoDB" id="6509908at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032218">
        <property type="term" value="P:riboflavin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF177">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER MCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="326"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="356"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="394"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="49"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="456" mass="49898" checksum="165713B28957D470" modified="2011-11-16" version="1" fragment="single">MSSISTCSTAEVIRLNTLEATVSTESQISEASIHEEQDSFPDGGFRAYSVVFGSFLGLVANFGTLNSMGVIQTYVATHQLTDVPTSTVSWIFSIYLALSFANCVIVGPFFDVKGATWPLIVGTIMMFGGFMAVANATTVWQFILSLSICVGIGNSLNITPLIGVLSHWFNLKRGSAIGMATMGGSIGGIIVPIMLKSLYPKFGFVWSTRILGFFCLGCMIFSIFLCRERVTRTLETREDQRHGKFKTMLGEVKEFFDFKALLDPTYAFCIAGTFAVEISLLSMLTYLASYAIAQGMDESKSYVLITIFNATGVLGRLVPGYLSDKLGYYNIMIIMLFGFTLSMFVIWLPFGHIVSALYVFASVSGFFSSSILSLTPVCLSTITPVRKFGSRYGLLYLFVSTGNLFGIPISGAIIGEGSKHQYNMFALFCCLFGILGTIGWITSRYYIVGMKLNVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT79</accession>
    <name>G3AT79_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 7 beta-sandwich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_52041</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
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        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376876.1">
        <property type="nucleotide sequence ID" value="XM_007376814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT79"/>
    <dbReference type="STRING" id="619300.G3AT79"/>
    <dbReference type="GeneID" id="18871622"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52041"/>
    <dbReference type="eggNOG" id="ENOG502RQ1V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082179_0_0_1"/>
    <dbReference type="InParanoid" id="G3AT79"/>
    <dbReference type="OMA" id="IFCICIT"/>
    <dbReference type="OrthoDB" id="4085072at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019008">
        <property type="entry name" value="Beta_sandwich_EMC7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09430">
        <property type="entry name" value="EMC7_beta-sandw"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442613" description="ER membrane protein complex subunit 7 beta-sandwich domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="ER membrane protein complex subunit 7 beta-sandwich" evidence="4">
        <location>
            <begin position="51"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="232" mass="26818" checksum="9B27DDAC29762196" modified="2011-11-16" version="1">MTRLINIITVLFSSVLVSAIGFQGQIINIPKQVNELYHEKNVVVVNGDNYPARIKVDLYNLESDEKTTSLVQQDYSFKFQDLKAGIYELIVNSYDFEFYTNRYRILVNDEIILAFEDRLGENSYNKTSEVNITKTPLTINFKYTKQFYENPQGSIGDWFLNGPFGFIFRNRVYTIIFTVMMGLSVAPYVLKWINPELAKELQEIQVQASKQTLEKAPAPSKSQPQGNIRKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATC0</accession>
    <name>G3ATC0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_156273</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007376854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATC0"/>
    <dbReference type="STRING" id="619300.G3ATC0"/>
    <dbReference type="GeneID" id="18871047"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_156273"/>
    <dbReference type="eggNOG" id="KOG0134">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012153_2_1_1"/>
    <dbReference type="InParanoid" id="G3ATC0"/>
    <dbReference type="OMA" id="GHITPWH"/>
    <dbReference type="OrthoDB" id="72788at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02932">
        <property type="entry name" value="OYE_YqiM_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044152">
        <property type="entry name" value="YqjM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43303">
        <property type="entry name" value="NADPH DEHYDROGENASE C23G7.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43303:SF4">
        <property type="entry name" value="NADPH DEHYDROGENASE C23G7.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="7">
        <location>
            <begin position="45"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="433" mass="47763" checksum="55FDE732774671F8" modified="2011-11-16" version="1">MAGTKEIYSSDEAERSTAVPYYTPRQPVPAGTFLPKSANDYIPRIFQPIAIGSLEIQNRIGVSPMCTYSADDNLEATPFHLIHYGAIVSRGPGITIVESTAVSHDGALSPHDLGIWTDEQAQKLKSIVDYAHSQDQKIALQINHGGRKASGQPLFLHLEQIADESVGGWPSKVVGPSSVQYRKYGNYLTPRELTKSEIKSIVQDFGDAAKRAVEISGFDAIEIHGAHGYLINQFYSPISNKRTDEYGGSFENRIRFLTEIIDNVKSKIPESIPVFLRISAVENSPNPEAWTIEQSQQLADVATQHGISMIDISSGGNDSQQVSRSELNSKDTKLPVHVPLARAIKQHVGNKIVVATVGGLHEDPVLVKDLLEQGVFDLALIGRGFMRNPGLVWEFADKLGVRTNQARQFEWGFYPMKEQIVNLMARAANFEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATF0</accession>
    <name>G3ATF0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62823</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376946.1">
        <property type="nucleotide sequence ID" value="XM_007376884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATF0"/>
    <dbReference type="GeneID" id="18874482"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62823"/>
    <dbReference type="HOGENOM" id="CLU_2533799_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATF0"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="84" mass="9865" checksum="7C57B996A6017913" modified="2011-11-16" version="1" fragment="single">MTGLRSGLISQRHSLIEFHWDSGIQISINPKRIYKLPVNDRIYFQIQAFLEDEYLLPKLHRVSIEGYHYLINRIGDGIPVFLPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATS5</accession>
    <name>G3ATS5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_63148</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNAP-25 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377272.1">
        <property type="nucleotide sequence ID" value="XM_007377210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATS5"/>
    <dbReference type="FunCoup" id="G3ATS5">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATS5"/>
    <dbReference type="GeneID" id="18874617"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63148"/>
    <dbReference type="eggNOG" id="KOG3065">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020823_1_0_1"/>
    <dbReference type="InParanoid" id="G3ATS5"/>
    <dbReference type="OMA" id="LDINVHM"/>
    <dbReference type="OrthoDB" id="18679at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035493">
        <property type="term" value="P:SNARE complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd15857">
        <property type="entry name" value="SNARE_SEC9C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15886">
        <property type="entry name" value="SNARE_SEC9N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000048">
        <property type="entry name" value="Protein transport protein SEC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305">
        <property type="entry name" value="SYNAPTOSOMAL ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305:SF9">
        <property type="entry name" value="SYNAPTOSOMAL-ASSOCIATED PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="3">
        <location>
            <begin position="368"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="52"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="75"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="133"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="431" mass="48887" checksum="79D1E6A590BA4926" modified="2011-11-16" version="1">MGLKKMFQKKEPTEAELREDLNRAGISTKSTNGRQEKFGAFKNYAQERASMKPGFAPVNNPYANICQPGGPGPANPYANNVGTDGASSSPYSQQQQQQSPYSQQSAYSQPQQSNPYGAQRLANQQQGAYGGSSREDPYSRRSSRTRPDDTESLDLNAIPTHPMHASRKPVRKYLPEDDDLNLEEEEDLNLDIEPEQEQVNSEDEEIEAIRQDIRFTKQESVQSTRNTLRMAQEADASGTNTLGMLGSQSERLYNAEQNLLLADTQTKIADEKVKQLHRLNRSIFIPASGNPFNKKARLRQQEEKIKAQKAQEKYLRESHRSEMYASEQRIKQGITTNATSSDVYAKYQGEKDLQAAQRYQFENDSEDDEMEKEIAGNLDQIGQYTKKLNSMAKTMGKEVESQNVRLRKIEEDADRLDINVHMNNTRLSNIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATT6</accession>
    <name>G3ATT6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">3'(2'),5'-bisphosphate nucleotidase</fullName>
            <ecNumber evidence="3 14">3.1.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_57124</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'-phosphate (PAP) to AMP.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Phosphatase that converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'-phosphate (PAP) to AMP. Regulates the flux of sulfur in the sulfur-activation pathway by converting PAPS to APS. Involved in salt tolerance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>3'-phosphoadenylyl sulfate + H2O = adenosine 5'-phosphosulfate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77639"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:77640"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>adenosine 2',5'-bisphosphate + H2O = AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77643"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:194156"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:77644"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>adenosine 3',5'-bisphosphate + H2O = AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:10041"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.7" evidence="3 14"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377284.1">
        <property type="nucleotide sequence ID" value="XM_007377222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATT6"/>
    <dbReference type="FunCoup" id="G3ATT6">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATT6"/>
    <dbReference type="GeneID" id="18872096"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57124"/>
    <dbReference type="eggNOG" id="KOG1528">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033446_2_1_1"/>
    <dbReference type="InParanoid" id="G3ATT6"/>
    <dbReference type="OMA" id="MSYQQER"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008441">
        <property type="term" value="F:3'(2'),5'-bisphosphate nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052829">
        <property type="term" value="F:inositol-1,3,4-trisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004441">
        <property type="term" value="F:inositol-1,4-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042538">
        <property type="term" value="P:hyperosmotic salinity response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043647">
        <property type="term" value="P:inositol phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016078">
        <property type="term" value="P:tRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01517">
        <property type="entry name" value="PAP_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000015">
        <property type="entry name" value="3',5'-bisphosphate nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000003">
        <property type="entry name" value="PAP-specific phosphatase HAL2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006239">
        <property type="entry name" value="DPNP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051090">
        <property type="entry name" value="Inositol_monoP_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01330">
        <property type="entry name" value="bisphos_HAL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43200:SF6">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43200">
        <property type="entry name" value="PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 13 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="13">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU368076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW30313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="351" mass="37782" checksum="1466FB2882DB887F" modified="2011-11-16" version="1">MATSTHPYAKELEIATLAVKRASILTKKLSDSIVTLQQSGTLTKDDKSPVTVGDFASQAIVNHAIKLNFPNDEIVGEEDSQDLQDSPLTSQILDLINQVQQETSEYDDKIGKLSNLKEITTSIDFGDSQGGSTGRFWALDPIDGTKGFLRGDQFAVCLALIENGKVVLGVIGCPNLPQIIHSNDKHEGIVGGLYSAVKGVGSYYSALFTKGFTPLDQQQKISMKSTSNPSELKVVEGVEKGHSSHSTQAQIKQILGITTPAINLDSQVKYCVLASGQSDIYLRLPVSDTYREKIWDHAAGNILIYESGGQVGDIEGNPLNFGTGRTLQSKGVIAGNKQVFDSVIKAVQQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATV8</accession>
    <name>G3ATV8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex component Sec8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_157214</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SEC8 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377304.1">
        <property type="nucleotide sequence ID" value="XM_007377242.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATV8"/>
    <dbReference type="FunCoup" id="G3ATV8">
        <property type="interactions" value="284"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATV8"/>
    <dbReference type="GeneID" id="18871069"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157214"/>
    <dbReference type="eggNOG" id="KOG3691">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004025_2_0_1"/>
    <dbReference type="InParanoid" id="G3ATV8"/>
    <dbReference type="OMA" id="HMEVRCR"/>
    <dbReference type="OrthoDB" id="272977at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090522">
        <property type="term" value="P:vesicle tethering involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039682">
        <property type="entry name" value="Sec8/EXOC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007191">
        <property type="entry name" value="Sec8_exocyst_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048630">
        <property type="entry name" value="Sec8_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20652">
        <property type="entry name" value="Sec8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04048">
        <property type="entry name" value="Sec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="3 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec8 N-terminal" evidence="6">
        <location>
            <begin position="27"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex component Sec8 middle helical bundle" evidence="7">
        <location>
            <begin position="325"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1102" mass="126124" checksum="0D27762F753EBB96" modified="2011-11-16" version="1">MTSRRLSFSMPHRGSRKSQAMDDAISELKQIYDSIRYDWPQILDHRANAIETAISLLDDSSVGLAHRLPEFENLKQQTEVGLRNVVNEHHEIFNNSIGSYHMLLSTLRESQEDSTEIKAYLESSNKEIHDRSDLLSELSQTSARYSELIDVLDAMAELTSIPDKIEQLVIDKKIHEVYDVISNGYKTAEKYNLWSLPAMAGIQSYLESQSNKLFDMIIDELQNEIYLKQTRITTDTTANISWQSIFNSSAPQLASLVSLINSKNLEQYVSNAANLDITEIVDFLTQPVQKFILHQLPELHTHSIMSDKGTEVKDDYQFLLDTKMNSNSSSFYYIYMLLRTASKLSRLHQVTEILIDSNQSELHGLINRTTEEIKSKNSTALSKLTNIKHHSEKSTLLEIMGNSNFNDSAVVVLEELFGSIFVKCLAVFHRHKVVNEIVRIIEEGQNLTSNTSMLSIWNVIKKELRTMMLNYIYDDSLVHANIINEPTTRAKDQSKIYKVITSRKNLFRFEDVTYDTSSRTATEILGIVTDVFPGFNMSGDNKELEKTKDELLGSSASPYIQTEQFNAMIDVLVPKNLFNMRVILEFFLIFIEGSQRVFANFEHSSDVTPHSKSSLQFFNDFMKISFMGYVKDGIEISFGEQVGGTFTREKPLHSTGLKLDLISLNQASDINVIANVKSDSTHNVIIYENAFNFKKLYLELCLMFNTSLTYRQDYSDVVLKVLDNFAREYHNLYHELLSAGDSTANNNRPVSQTSKWMNIPALREISMKILQAHVQNVPRSRMEELFHAESQVLLFEGNDDLNAVEKDDLLDPDSYSQVLYLLLTTSWVLSWLPLIKKETNTKNKDESDSDTDLHIAMIDKLRSNWSFLENGRMPINFTANSSDIIHHNVFLCLGPDKVSKFDQIVKTFETIRDGTLLALRYDLRCKAIYYTTNSYKHVDWTPTNEPGDADHYIGLFNQEVFAVDSKLSSMLSNVERETVFIGLTKFLNDLFIQGSINLKKVNNNGIKRILLNISTVQQMLRSVATNPEVIDFTKSSVYFEMFTLNEFTLINRFRSNEQRYTKSACNNLARLIYSEKLADGQGSSFNKGKYNDLLKKIDELVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU29</accession>
    <name>G3AU29_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Formamidopyrimidine-DNA glycosylase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_57196</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.</text>
            <dbReference type="EC" id="3.2.2.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPG family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377376.1">
        <property type="nucleotide sequence ID" value="XM_007377314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU29"/>
    <dbReference type="STRING" id="619300.G3AU29"/>
    <dbReference type="GeneID" id="18872104"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57196"/>
    <dbReference type="eggNOG" id="ENOG502QVDB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038423_0_3_1"/>
    <dbReference type="InParanoid" id="G3AU29"/>
    <dbReference type="OMA" id="EKFPEHW"/>
    <dbReference type="OrthoDB" id="444592at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008534">
        <property type="term" value="F:oxidized purine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08972">
        <property type="entry name" value="PF_Nei_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.50:FF:000009">
        <property type="entry name" value="Formamidopyrimidine-DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.190.10">
        <property type="entry name" value="MutM-like, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012319">
        <property type="entry name" value="FPG_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035937">
        <property type="entry name" value="FPG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015886">
        <property type="entry name" value="H2TH_FPG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22993">
        <property type="entry name" value="FORMAMIDOPYRIMIDINE-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22993:SF9">
        <property type="entry name" value="FORMAMIDOPYRIMIDINE-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01149">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06831">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00898">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01232">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81624">
        <property type="entry name" value="N-terminal domain of MutM-like DNA repair proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51068">
        <property type="entry name" value="FPG_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Formamidopyrimidine-DNA glycosylase catalytic" evidence="10">
        <location>
            <begin position="2"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="384" mass="43927" checksum="BD3A98C0FAC1A6A7" modified="2011-11-16" version="1">MPEVAEVAHVCAQLRRNILGHRIKSVLASNDPLIFPFLKLSTDPDKELSTFQNALTGCSVSSIGRHGKYFWMRLQPRDKSSLVLLMHFGMTGMVKLRNINSHLIFMENGGDKKRLDKLKQTEKEPSVADEEVTWPPKFTKFEMTFEDGDNQVEFAFVDPRRLARIRLLTGDSIQNDLDLLNHPPLDVLGPDYSKPRELLKQEQFTMGDPDPDNHGRPRLTLAEFNKLILTKKKPIKSLLLDQNCFAGVGNWVADEILFNARIHPAEVISNKIDNVTTIHPVIERLYENLVYVCETAVKVEGNVDHFPKHWLMLHRWGKSRKKEKPKTNDGYLLDYVTVGGRTSCYSPELQKMLKQDSKIKATTDKQKFPQGSSKECYIWSLGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU66</accession>
    <name>G3AU66_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_157476</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377413.1">
        <property type="nucleotide sequence ID" value="XM_007377351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU66"/>
    <dbReference type="GeneID" id="18871083"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157476"/>
    <dbReference type="eggNOG" id="ENOG502RQ34">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1042055_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU66"/>
    <dbReference type="OMA" id="MELYNSQ"/>
    <dbReference type="OrthoDB" id="4094308at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="253" mass="28818" checksum="50295E6A5AF1CB5A" modified="2011-11-16" version="1" fragment="single">MDEDPLLGTIVRIPLSVVDKETSGTIKSCPLWLHVSPATYKASNDPKFVNVSITAVYQEGYFHTTITKDVIGNIFRKSSGIESEDYYSVITSLFPLDLETLIEVNSEESYKELSLSAKLVASSSDYDENQDILELGDSDPNKQGITITIKREGKLTVTVGSIDLPGIEFDKYDYLQEEGDLFNWLSLYNHQNKLLLDNLRDSKVEVETLKQENYLLEKNSSIAKKDFDYIIKDVESRFYQVLDSKKDKIWELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU71</accession>
    <name>G3AU71_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63282</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377418.1">
        <property type="nucleotide sequence ID" value="XM_007377356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU71"/>
    <dbReference type="FunCoup" id="G3AU71">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="GeneID" id="18874676"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63282"/>
    <dbReference type="eggNOG" id="ENOG502RQ2E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097218_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU71"/>
    <dbReference type="OMA" id="LNFTIWE"/>
    <dbReference type="OrthoDB" id="4022168at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="226" mass="25625" checksum="030A27FA6C388DA1" modified="2011-11-16" version="1">MSTTPSPTKRTPLSSKTFNISPTPLKRPNPVHLPSRLSPTKRLAPARNTPSPPKKSTGLNFTIWEDKTEKPEFPEQEYPCSNKQNNHDQENILQPKKNDIIRSSVRSPLADLSINEYPGFVSYGGPEFKLGELYQPPNFNNEFKSLHKHSSLPNYLTPSRKHSGRDKYLVVSGTTPDIDETELLLIEKQQRLSNKVIRKHTRSLSVGKNEAKLSLIRKNNFSILSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU76</accession>
    <name>G3AU76_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Pre-mRNA-processing protein 45</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_63286</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SNW family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377423.1">
        <property type="nucleotide sequence ID" value="XM_007377361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU76"/>
    <dbReference type="STRING" id="619300.G3AU76"/>
    <dbReference type="GeneID" id="18874680"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63286"/>
    <dbReference type="eggNOG" id="KOG2441">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085411_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU76"/>
    <dbReference type="OrthoDB" id="666364at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017862">
        <property type="entry name" value="SKI-int_prot_SKIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004015">
        <property type="entry name" value="SKI-int_prot_SKIP_SNW-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12096">
        <property type="entry name" value="NUCLEAR PROTEIN SKIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02731">
        <property type="entry name" value="SKIP_SNW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="SKI-interacting protein SKIP SNW" evidence="4">
        <location>
            <begin position="111"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="191" mass="22442" checksum="6E854E771A6C0A74" modified="2011-11-16" version="1" fragment="single">MFSTLLSRPKNSSYDPNYHFRIDTTTNDNKQVLVLPRQTDLQALDFSKPIDDEEVTYESTIPLKKRYPNLTHSFPRPSLDDLEVRSVIEETRSIIAKLITPDQPEENVQHIQYSSKNIIDTDERVIQIRKFQVDPMLPPQFKLRKNRHKTQTEDVPIVKPTNQEKLTKEDRQKWNIPVAVSNWKNNQGFTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUX3</accession>
    <name>G3AUX3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Factor arrest protein 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_144103</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377830.1">
        <property type="nucleotide sequence ID" value="XM_007377768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUX3"/>
    <dbReference type="FunCoup" id="G3AUX3">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUX3"/>
    <dbReference type="GeneID" id="18870599"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144103"/>
    <dbReference type="eggNOG" id="KOG3680">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003184_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUX3"/>
    <dbReference type="OMA" id="KMTRAMR"/>
    <dbReference type="OrthoDB" id="18234at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040185">
        <property type="entry name" value="Far11/STRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021819">
        <property type="entry name" value="Far11/STRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012486">
        <property type="entry name" value="Far11/STRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13239:SF4">
        <property type="entry name" value="AT25231P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13239">
        <property type="entry name" value="PROTEIN REQUIRED FOR HYPHAL ANASTOMOSIS HAM-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11882">
        <property type="entry name" value="DUF3402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07923">
        <property type="entry name" value="N1221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01293">
        <property type="entry name" value="DUF3402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01292">
        <property type="entry name" value="N1221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Far11/STRP N-terminal" evidence="2">
        <location>
            <begin position="102"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Far11/STRP C-terminal" evidence="3">
        <location>
            <begin position="516"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="962" mass="112401" checksum="F7C9634FFBB70E72" modified="2011-11-16" version="1">MMQDELISIDQLEDQIIDHPDASTEKELHESLDETFQKKLNARAERLPNDGANSLHAHHHHHHHHESNSSSSGSKNDDNSTGGRNDADIAGPQIGLSFLITKSHLQYEYQEIGSLETEINEWFTLNDLKILGGLSNMYQLYERSHEGNDITLFLKRNIEVIDEANLLDTNAAVSISLKILLYFAFGEYATKKTRQQQLDQIKKNSFVLLQLQVYKPLIRLIKDLVDKRIEFDKDIDTDEEEEQSTLSQKVEADYFKVLTLLFFLTQVNLQDRDNEHSIEFRKYLGEVDFLTTIFRFIEHWKWYPNNSYRIRYLITLVNNLILFELGGATNLKACDNFLVDLHQIKNKKGRDLGETVLTCSPLDYFAFREDLLDKFPLYENTELKAFDFTEFTRTLEREDDIDDTSSIHSHESTIEGKYKYFMAVNSFSNSLSNLMETPKTTKSHTVLSQLPTQTVHIATPVPSPTFNASDYMSGGEKVRRSYQVNQAMPFIYPNLPAASTQQEVDDESPSSTVTVPLAMKEADEILKNSIYESYSIKRLWNEREKFMIQERGYVNEYQEEQPKDEFDYDYDDLKQRFPEKMDVIELLQRVDKMYAANVSRLYTVVEVLMETIKVNRMDYNLNFAELELNPETSFLSRNGVPTPGDAKLKSIEQTKEKIRYVLTSQLEVLNVKEITLKASTNIVLLLLKWFKISHVLKYYYLSSILFDQQFFTVALDYLSRSFNNANLQSLSKDEAKKDDLTEYEILINQNKLMNPKVELPTYDFFNNCLQVFPDGNYRYKFINKEFISTLPEQVDANNINHVYIKHYNSNFAFILSNMLKITNKILIKNQTQRIFTLNDLKPSELYKMILMNYDNDAFTKPILKTLKKLIPYQGRKWKSINMDLISQIYLNLKLSMKDHWLSGKDLESDFNNSYDQEIALRGLLQFYNMRNFPKQMEKIGYQVNHDLDIPVMNLNDEDDGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV16</accession>
    <name>G3AV16_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutamine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_63714</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tRNA(Gln) + L-glutamine + ATP = L-glutaminyl-tRNA(Gln) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20121"/>
            <dbReference type="Rhea" id="RHEA-COMP:9662"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.18" evidence="2"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377856.1">
        <property type="nucleotide sequence ID" value="XM_007377794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV16"/>
    <dbReference type="FunCoup" id="G3AV16">
        <property type="interactions" value="1312"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV16"/>
    <dbReference type="GeneID" id="18874880"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63714"/>
    <dbReference type="eggNOG" id="KOG1148">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001882_2_3_1"/>
    <dbReference type="InParanoid" id="G3AV16"/>
    <dbReference type="OMA" id="TWCIYPM"/>
    <dbReference type="OrthoDB" id="10250478at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004819">
        <property type="term" value="F:glutamine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006425">
        <property type="term" value="P:glutaminyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00807">
        <property type="entry name" value="GlnRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1160.10:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000007">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.800.10:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000015">
        <property type="entry name" value="Glutaminyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000183">
        <property type="entry name" value="Glutaminyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004514">
        <property type="entry name" value="Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050132">
        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00440">
        <property type="entry name" value="glnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF4">
        <property type="entry name" value="GLUTAMINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="10">
        <location>
            <begin position="34"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib anti-codon binding" evidence="11">
        <location>
            <begin position="351"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA synthetases class I (E and Q) anti-codon binding" evidence="12">
        <location>
            <begin position="463"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW30090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="582" mass="66861" checksum="642707516F11215B" modified="2011-11-16" version="1">MFSEGFLGDLHKPGEEPQMYPELLGPHRDFIKGKVHTRFPPEPNGFLHIGHSKAIMVNFGFAQFHKGNCYLRFDDTNPEAEEEVYFNSIKDMVAWLGYEPWKITYSSDYFDQLYDLAVKLIESDKAYVCHCTADEVKASRGMKEDGTLGGERKACAHRSQSIEHNLKEFTNMRDGKYNVGEATLRMKQDLESDSPQMWDLVAYRVLNTPHHRTGDKWKIYPTYDFTHCLVDSFENITHSLCTTEFILSRESYEWLCDTLHVYRPPQREYGRLNITGTIMSKRKIAKLVNEKYVRGWDDPRLYTLEGIKRRGVPPGAILSFINTLGVTTSTTNIQVVRFESAVRNYLDQTTPRLMMVLNPIEVVIDNLPEDFSLDIEIPYKPGKGEEKFGYRKVKFSNKIYIDANDVRFEAADSNYFRLAPGQPVGLIKVPYNISFKSVEKVGDITRVHVNYESENHKKPKTYIQWVPQDSAIHINQVRIYNQLFNSENPSAHPDGYLKDINPDSEHVITNAVVEPSFKEVEANSPLNIEIPGSDFNIKEAKGANTIRFQALREGYFCVDKDSSSDKLILNRIVTLKEDAAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVP9</accession>
    <name>G3AVP9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 14">Ribulose-phosphate 3-epimerase</fullName>
            <ecNumber evidence="8 14">5.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_63833</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>D-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13677"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="16">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate degradation; pentose phosphate pathway; D-xylulose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 14">Belongs to the ribulose-phosphate 3-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.1" evidence="8 14"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377980.1">
        <property type="nucleotide sequence ID" value="XM_007377918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVP9"/>
    <dbReference type="FunCoup" id="G3AVP9">
        <property type="interactions" value="478"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVP9"/>
    <dbReference type="GeneID" id="18874935"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63833"/>
    <dbReference type="eggNOG" id="KOG3111">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054856_0_1_1"/>
    <dbReference type="InParanoid" id="G3AVP9"/>
    <dbReference type="OMA" id="CHLMIED"/>
    <dbReference type="OrthoDB" id="1927044at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00411"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004750">
        <property type="term" value="F:D-ribulose-phosphate 3-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000074">
        <property type="entry name" value="Ribulose-phosphate 3-epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02227">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026019">
        <property type="entry name" value="Ribul_P_3_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004076">
        <property type="entry name" value="PRK05581.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01163">
        <property type="entry name" value="rpe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="RIBULOSE-5-PHOSPHATE-3-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001461">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01085">
        <property type="entry name" value="RIBUL_P_3_EPIMER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="13 16" id="KW-0170">Cobalt</keyword>
    <keyword evidence="12 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="16" id="KW-0464">Manganese</keyword>
    <keyword evidence="10 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 16" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="150"/>
            <end position="153"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="201"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00009541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00013920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR001461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW30214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="233" mass="25131" checksum="02B6B5149702A3F3" modified="2011-11-16" version="1">MVKPIIAPSILAADFANLGCNCKKVVDTGDVDWLHIDVMDGHFVPNISLGSPVITSLRKAFPRDDAKPIVFDCHMMVSEPEQWIADIAKAGGDSYTFHFEATKDPAKVIKKIKEHGLKAAMAVKPGTPVEEVYPYAADLDMVLVMTVEPGFGGQKFMPDMMEKVEKLRAKYPNLDVQVDGGLAKDTIEPAAKAGANVIVAGTSVFKPEDPKEVTSFLKDAVTKEIAARASATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVR4</accession>
    <name>G3AVR4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Superoxide dismutase</fullName>
            <ecNumber evidence="2 8">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_144825</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="2 8"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377995.1">
        <property type="nucleotide sequence ID" value="XM_007377933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVR4"/>
    <dbReference type="STRING" id="619300.G3AVR4"/>
    <dbReference type="GeneID" id="18870666"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144825"/>
    <dbReference type="eggNOG" id="KOG0876">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031625_2_1_1"/>
    <dbReference type="InParanoid" id="G3AVR4"/>
    <dbReference type="OMA" id="LNNWWNV"/>
    <dbReference type="OrthoDB" id="239262at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.990:FF:000001">
        <property type="entry name" value="Superoxide dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050265">
        <property type="entry name" value="Fe/Mn_Superoxide_Dismutase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001189">
        <property type="entry name" value="Mn/Fe_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019833">
        <property type="entry name" value="Mn/Fe_SOD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11404">
        <property type="entry name" value="SUPEROXIDE DISMUTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11404:SF6">
        <property type="entry name" value="SUPEROXIDE DISMUTASE [MN], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00081">
        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000349">
        <property type="entry name" value="SODismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01703">
        <property type="entry name" value="MNSODISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00088">
        <property type="entry name" value="SOD_MN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase N-terminal" evidence="9">
        <location>
            <begin position="5"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="10">
        <location>
            <begin position="98"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000349-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="202" mass="23179" checksum="DDF4ABEBD8E85EBB" modified="2011-11-16" version="1">MNQLQLPKLNYAFDSLEPYISAEIMQIHYLKHHQAYIDNYNAAMEDLSKARVKGDTKQIVTLQQNIRFNGGGYINHVLFWESLSPVATGGGEKPTDKSSLGRQIIVQYESVDKLIEITTEKLVSIQGSGWAWIVKDTQNGGKLDVVTTVNQDIVLPPLVPLIGIDAWEHSYYLQYQNVKREYFDAMWNVVNWKEAERKFNEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE14</accession>
    <name>G3AE14_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Bicarbonate transporter-like transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_53767</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the anion exchanger (TC 2.A.31) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372960.1">
        <property type="nucleotide sequence ID" value="XM_007372898.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE14"/>
    <dbReference type="FunCoup" id="G3AE14">
        <property type="interactions" value="406"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AE14"/>
    <dbReference type="GeneID" id="18871810"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53767"/>
    <dbReference type="eggNOG" id="KOG1172">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002289_7_2_1"/>
    <dbReference type="InParanoid" id="G3AE14"/>
    <dbReference type="OMA" id="ADAFNYR"/>
    <dbReference type="OrthoDB" id="1735926at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080139">
        <property type="term" value="F:borate efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050801">
        <property type="term" value="P:monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000003">
        <property type="entry name" value="Anion exchange family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF82">
        <property type="entry name" value="BORON TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="391"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="455"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="479"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="8">
        <location>
            <begin position="14"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="8">
        <location>
            <begin position="208"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="557" mass="62744" checksum="E756072220D2408E" modified="2011-11-16" version="1">MRITFKSFNYIGYGIYNDIKTRLPYYKSDFADAFNYRVIPSTIFIFFTNLLPAIAFAQDMFDKTDHAYGLNEVLMSSALAGVVFGLFSGQPLCIVGVTGPISIFSYTVYELITPRGTPYFPFMCWIYLWSMVFHIIIAVGNYISFLKIISLFSCDVFGFFINIVYIQKGIQILNNQFKVDLASGYCSVMISLLMIICGVGSILFGNYLHYFKPWVRRVFVDYGVVLSVIFFTGFIHFGGHLDETVLATLPTTSSFVPTHTGSDRAHGWFIHFWPGDNISVGDVFLAIPFAILLTFLFYFDHNVSSLMCQSKEYPLTKPASFHWDFLLLGLTTGLAGILGLPAPNGLIPQAPLHTDSLVVHDNLGKKLSVVEQRVTNTVQGALTFLMMSPPFLIVLGLIPQAVLSGLFFIMAITGLHGNIVTNRIRYCFLENSYIENDPSCPQVWKDIHELPNKKWFYVYTALEVVAGVAEFAITLTKGAVGFPGVLLFFAFCAKWVWPLIIPREDLDKLDGEVADEFIIKNLDITNHIKREQRDLEAKGDDVNLQMYEMSDGNSRYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE55</accession>
    <name>G3AE55_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_132742</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373001.1">
        <property type="nucleotide sequence ID" value="XM_007372939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE55"/>
    <dbReference type="GeneID" id="18869688"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132742"/>
    <dbReference type="eggNOG" id="ENOG502RWDV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012596_0_1_1"/>
    <dbReference type="InParanoid" id="G3AE55"/>
    <dbReference type="OMA" id="FYGFTFC"/>
    <dbReference type="OrthoDB" id="410267at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576:SF158">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 12-LIKE CONSERVED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576">
        <property type="entry name" value="UNCHARACTERIZED NODULIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="246"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="366"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="396"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="443"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="491" mass="53592" checksum="F375E4C967D3B06A" modified="2011-11-16" version="1">MIALPRTVQKAILLLSCTFLGLTCGTLYMYSSYSPQFARQVNYTASDSSYIALSGTIGIAIGGPIAGILVDKRGYTAALALGGTLIISGYMIMRRQFDYTYHNLPLSCSLTLMIGIGSTAINSVTLKCCAVSFPSIRGVATSLPLSLYGLSALFYSVAASIFYPGDTSSFLGFVAGSIFCILLVCSPSIILADREHKLKRSAAKYHKSSHPDIKSDALPLSPSVTTDYQFGEAALSNESLLKNPKFWLLFIILGSLASLGQMYIYSVGYIVKALVSYSLRNEVDITPILLQETETIIQHDQQFQVGLLSIANCLGRIISGIMGDIITQSFNKSRTWLLFFPSIGMMITQLLSLTTRTYDNLPLNSLLTGLFYGFTFCIMPLIVGDTFGLDNFSYNWGVVNMAPILPSYYFTMLFGSIYDSKSQFSEQHGGLVCLLGNQCYNSIFKITLLVSIFAVIIVSILTIGPKLSLKTKKTLPLTSSPLKQEVNEKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEA9</accession>
    <name>G3AEA9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58851</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373055.1">
        <property type="nucleotide sequence ID" value="XM_007372993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEA9"/>
    <dbReference type="FunCoup" id="G3AEA9">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEA9"/>
    <dbReference type="GeneID" id="18872645"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58851"/>
    <dbReference type="eggNOG" id="ENOG502RZQV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092488_2_0_1"/>
    <dbReference type="InParanoid" id="G3AEA9"/>
    <dbReference type="OMA" id="EFLIHEW"/>
    <dbReference type="OrthoDB" id="2100652at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014807">
        <property type="entry name" value="Coa1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042432">
        <property type="entry name" value="Coa1_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28523">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28523:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08695">
        <property type="entry name" value="Coa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="186" mass="21326" checksum="83E055D05F091A25" modified="2011-11-16" version="1">MLQSFKRVHTPLRIATIGLRCVHTTPLLFNQQPIATAPQGVRRPPVSIDRPLPDPFEKKRQNRRYFWVYFVGVSLMCGLIFNYEKTGSPIITSTFYFLRRSSIARTHLGDEISYTSRWPWIVGPLNTVKGNIDIKFGVKGEKGTGEIRLRATRPNKAVPFEIGEFSLVVDDQVYDLTKDPEIEFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEG9</accession>
    <name>G3AEG9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_57797</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the complex I NDUFS4 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372143.1">
        <property type="nucleotide sequence ID" value="XM_007372081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEG9"/>
    <dbReference type="STRING" id="619300.G3AEG9"/>
    <dbReference type="GeneID" id="18872201"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57797"/>
    <dbReference type="eggNOG" id="KOG3389">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077196_2_0_1"/>
    <dbReference type="InParanoid" id="G3AEG9"/>
    <dbReference type="OMA" id="GTIMKFD"/>
    <dbReference type="OrthoDB" id="3089at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.190:FF:000001">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 21 kDa subunit mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.190">
        <property type="entry name" value="atu1810 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006885">
        <property type="entry name" value="NADH_UbQ_FeS_4_mit-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038532">
        <property type="entry name" value="NDUFS4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12219:SF8">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12219">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04800">
        <property type="entry name" value="NDUS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5 9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="158" mass="18140" checksum="AA0122B1666B4569" modified="2011-11-16" version="1">MLSRVSIKSIPATGVRCLSSTAFLRQKAVAEAEEFVTGKEIVSGAPKELVTNRVVRIYQEAKYATQSGVHNTRNWKLDWDVLGKGNRWENDLMGYQGSADYMQGTIMKFDTKEAAVRFAEGQNWDYYIQEPKERHFRKKEYAANFYHSAGPLKHIRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEM4</accession>
    <name>G3AEM4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_58856</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373062.1">
        <property type="nucleotide sequence ID" value="XM_007373000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEM4"/>
    <dbReference type="STRING" id="619300.G3AEM4"/>
    <dbReference type="GeneID" id="18872648"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58856"/>
    <dbReference type="eggNOG" id="ENOG502S7ZI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060554_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEM4"/>
    <dbReference type="OrthoDB" id="1939598at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089713">
        <property type="term" value="C:Cbf1-Met4-Met28 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14705">
        <property type="entry name" value="bZIP_Zip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13044">
        <property type="entry name" value="ACTIVATING TRANSCRIPTION FACTOR ATF 4/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13044:SF14">
        <property type="entry name" value="CRYPTOCEPHAL, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="140"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="121"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="165"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="128"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="222" mass="24640" checksum="9A54D0D69AD5B586" modified="2011-11-16" version="1">MVPSHSFDTHHRNTSRHDLIQQAPRNNRPEILSQDSGFLNLTDLPKFPVPPGAKSSLVNAGLSSNQIDLLSALVAQHQASLGNSIPEMTASSSIASTISGSPPMKGDSPVRQHSQLFLESNGPIGYVPSPTTSTYSNTPDPAELDKRRRNTAASARFRIKKKLKEKEMEGKIHNLTELISELENKLQQLEMENKLLRNLIIEKGSQKSDDEVKMLRERAKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AET4</accession>
    <name>G3AET4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial</fullName>
            <ecNumber evidence="19">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">SPAPADRAFT_58964</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 19">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="17">Flavinylated by SdhE, about 5% flavinylation occurs in the absence of SdhE.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 19">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 19">Mitochondrion inner membrane</location>
            <topology evidence="1 19">Peripheral membrane protein</topology>
            <orientation evidence="1 19">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="19"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373176.1">
        <property type="nucleotide sequence ID" value="XM_007373114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AET4"/>
    <dbReference type="FunCoup" id="G3AET4">
        <property type="interactions" value="766"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AET4"/>
    <dbReference type="GeneID" id="18872700"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58964"/>
    <dbReference type="eggNOG" id="KOG2403">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_6_1_1"/>
    <dbReference type="InParanoid" id="G3AET4"/>
    <dbReference type="OMA" id="NRCAETI"/>
    <dbReference type="OrthoDB" id="71672at2759"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000001">
        <property type="entry name" value="Mitochondrial succinate dehydrogenase flavoprotein subunit"/>
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    </dbReference>
    <dbReference type="FunFam" id="4.10.80.40:FF:000002">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:001062">
        <property type="entry name" value="Succinate dehydrogenase complex, subunit A, flavoprotein (Fp)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000026">
        <property type="entry name" value="Succinate dehydrogenase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.100:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase flavoprotein subunit (SdhA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.100">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.80.40">
        <property type="entry name" value="succinate dehydrogenase protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003952">
        <property type="entry name" value="FRD_SDH_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011281">
        <property type="entry name" value="Succ_DH_flav_su_fwd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014006">
        <property type="entry name" value="Succ_Dhase_FrdA_Gneg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01816">
        <property type="entry name" value="sdhA_forward"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01812">
        <property type="entry name" value="sdhA_frdA_Gneg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE 2 FLAVOPROTEIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632:SF51">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000171">
        <property type="entry name" value="SDHA_APRA_LASPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00504">
        <property type="entry name" value="FRD_SDH_FAD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 19" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 17 19" id="KW-0274">FAD</keyword>
    <keyword evidence="6 17 19" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 19" id="KW-0813">Transport</keyword>
    <keyword evidence="5 19" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="20">
        <location>
            <begin position="64"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal" evidence="21">
        <location>
            <begin position="514"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="69"/>
            <end position="74"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="92"/>
            <end position="107"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="442"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="453"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="458"/>
            <end position="459"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Tele-8alpha-FAD histidine" evidence="18">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000171-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="EGW35764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="649" mass="71026" checksum="9EFF4692086A0CCD" modified="2011-11-16" version="1">MLTSVRNQLGRSAAIKRSVLSTSVRALSCSTVRSAVVGSNVKGQDFNASKYMGQKYHIVDHEYDCLVVGAGGAGLRAAFGLASEGYKTACISKLFPTRSHTVAAQGGINAALGNMHPDDWHWHFYDTVKGSDWLGDQDAIHYMTKEAPKSIYELEHYGVPFSRNEEGRIYQRAFGGQSKEYGKGGQAYRTCAVADRTGHALLHSLYGQSLRHDTHFFIEFFAMDLMMQDGACIGAIAYNQEDGTLHRFFANRTVMATGGYGRAYFSCTSAHTCTGDGYAMVARAGLPLEDMEFVQFHPSGIYGSGCLITEGARGEGGFLLNSEGERFMERYAPSAKDLASRDVVSRAITMEINEGRGVGPDKDHMYLQLSHIPAAILKERLPGISETAHIFAGVDVTKEPIPILPTVHYNMGGIPTNWKGEVLKQGKDGKDEIVPGLLACGEVACASVHGANRLGANSLLDLVVFGRAVSHTIRDTLKPGAPIKTHEKDIGHDSIANLDKLRNANGTKNTADIRLDMQKTMQKGCAVFRTQETLDKCAKLIGEIDQSFADVKTTDRSMIWNSDLVETLELQNLLTCAAQTVNSAAARKESRGAHARDDFPDRDDVNWRKHSLSYQDKSGDKVRLAYRDVIAHTLDESDCKPVPPAKRVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF17</accession>
    <name>G3AF17_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zinc-ribbon 15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_57907</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372233.1">
        <property type="nucleotide sequence ID" value="XM_007372171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF17"/>
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        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF17"/>
    <dbReference type="GeneID" id="18872245"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57907"/>
    <dbReference type="eggNOG" id="ENOG502SDGY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115926_1_1_1"/>
    <dbReference type="InParanoid" id="G3AF17"/>
    <dbReference type="OMA" id="AVRTINC"/>
    <dbReference type="OrthoDB" id="5545479at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28139">
        <property type="entry name" value="UPF0768 PROTEIN YBL029C-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28139:SF1">
        <property type="entry name" value="UPF0768 PROTEIN YBL029C-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="9990" checksum="9B6AE9C9C1255D96" modified="2011-11-16" version="1">MDFCFFLVGFKEFNKPLPDVGNVACYCGHCHNQSAFPMRTINCITLFFIPVLPFYFHKRLRCNICGTTGTLNQEALERLKQGQPVAIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF41</accession>
    <name>G3AF41_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CipC-like antibiotic response protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59022</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373229.1">
        <property type="nucleotide sequence ID" value="XM_007373167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF41"/>
    <dbReference type="GeneID" id="18872723"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59022"/>
    <dbReference type="eggNOG" id="ENOG502S76S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143683_0_0_1"/>
    <dbReference type="InParanoid" id="G3AF41"/>
    <dbReference type="OMA" id="MAWGWEQ"/>
    <dbReference type="OrthoDB" id="9895617at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022234">
        <property type="entry name" value="DUF3759"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37450">
        <property type="entry name" value="CIPC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37450:SF1">
        <property type="entry name" value="CIPC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12585">
        <property type="entry name" value="DUF3759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="14016" checksum="F705C1AC9D841301" modified="2011-11-16" version="1">MFDFLKDNHEQVYGEKHEGKFSHELVAGAASFEAVKLFEDKQRKEGKPVSHAFAKELLAGIAGGEVDKLFETKGLDYLDRDQAKSQAVDNAQRAYDSHYGNQEQWHPDSQPPFDYQSDQYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFB5</accession>
    <name>G3AFB5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Succinate-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="8">1.2.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_58029</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 8">
            <text>succinate semialdehyde + NAD(+) + H2O = succinate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13217"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 8">
            <text>succinate semialdehyde + NADP(+) + H2O = succinate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13213"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid degradation; 4-aminobutanoate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.16" evidence="8"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372316.1">
        <property type="nucleotide sequence ID" value="XM_007372254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFB5"/>
    <dbReference type="STRING" id="619300.G3AFB5"/>
    <dbReference type="GeneID" id="18872291"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58029"/>
    <dbReference type="eggNOG" id="KOG2451">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_5_1_1"/>
    <dbReference type="InParanoid" id="G3AFB5"/>
    <dbReference type="OMA" id="IGELFCK"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="UniPathway" id="UPA00733"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036243">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07103">
        <property type="entry name" value="ALDH_F5_SSADH_GabD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000004">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000005">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050740">
        <property type="entry name" value="Aldehyde_DH_Superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010102">
        <property type="entry name" value="Succ_semiAld_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01780">
        <property type="entry name" value="SSADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353:SF5">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="27"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00052698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="499" mass="53469" checksum="40D929049874AEC2" modified="2011-11-16" version="1">MESSSKIISSLKRPELVQTQAYVNGNWVSSTSGDSFAVNNPALYPKSEAKIAEVTSMTLHDFNNAIEAAHRAFSTFQKTSGRYRAGLLHDLYQLMMENKDDLAKLVVLENGKPYADALGEVTYAASFFQWYSEEAPRVYGDIIPSSNSTNRILAIKQPIGVCGIMTPWNFPLAMITRKLGAAIAAGCTSVIKPASETPLSATALGYLSQLAKIPPGVVNILPAAESSSVGKLITEHPLVSKVSFTGSTAVGKILMKQSASTLKKLSMELGGNAPLIVFNDADIDKAVTGAIASKFRSSGQTCVCANRLFVHEDVYEEFSNKLVAKLEETTTLGNGFSPDVTHGPVIHDRSMKKVRQHIEDAVSKGAKVLYGGSTRPDLGENFHELTVLGNVTTDMLIAQEETFGPVAPLIKFSTDEEVIQLANDTRVGLAGYFYANDVTRIFKVAESLKVGMIGVNTGAISEASLPFGGVSESGFGREGSKYGIDDYLITKSVVLGGID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFD4</accession>
    <name>G3AFD4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_58051</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S3 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372335.1">
        <property type="nucleotide sequence ID" value="XM_007372273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFD4"/>
    <dbReference type="FunCoup" id="G3AFD4">
        <property type="interactions" value="1214"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFD4"/>
    <dbReference type="GeneID" id="18872299"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58051"/>
    <dbReference type="eggNOG" id="KOG2581">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019858_1_2_1"/>
    <dbReference type="InParanoid" id="G3AFD4"/>
    <dbReference type="OMA" id="AKVYFYF"/>
    <dbReference type="OrthoDB" id="1713558at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050756">
        <property type="entry name" value="CSN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013586">
        <property type="entry name" value="PSMD3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057985">
        <property type="entry name" value="TPR_PSMD3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758:SF2">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3/COP9 SIGNALOSOME COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08375">
        <property type="entry name" value="Rpn3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25573">
        <property type="entry name" value="TPR_PSMD3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="237"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="480" mass="54985" checksum="54D1BDA5A0C4D8BF" modified="2011-11-16" version="1">MADTKADVLMKDTEEEVNASVFQDLIHEIESSFSLLGKAASSFDNRYISKVFRDLGPLRRKLANNDKVLPTIITNLYPANHPSREYLLRFVGNVEKSESMDVESENGQAIVPEVELYVHLLAQVYLLDKEKLSELNELSDIVIGLMKSFNRRSLDFIQAKVWFYVCRVKELLGDLYTIRPELLYGLRTATLRHDIETTASIITLLLRNYLLCHDINQASNLVEKVEFPENAGNALVARYYYYLARINAIQLDYSTAHECVIAAIRKAPQTHLAKGFIQSATKLSIVIELLMGDIPALKVFKEHQNFEPYFNVTRAVRLGDLKLFGEVLKKYETNFKNDDNFTLVSRLRQNVIKTGIRIISLSYSKISLKDICIKLHLDSEESTEYIVSKAIRDGVIEATINHQKGYMQSNELLDVYSTKLPQTEFDQRIKFCLSLHNESVKAMRYPNDNDKQNVTKEVEASEEIELLKAIEEGDLDDFMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFH0</accession>
    <name>G3AFH0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zinc-finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_145660</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372371.1">
        <property type="nucleotide sequence ID" value="XM_007372309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFH0"/>
    <dbReference type="STRING" id="619300.G3AFH0"/>
    <dbReference type="GeneID" id="18870695"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_145660"/>
    <dbReference type="eggNOG" id="ENOG502RZ2S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006926_1_1_1"/>
    <dbReference type="InParanoid" id="G3AFH0"/>
    <dbReference type="OMA" id="NDTESMY"/>
    <dbReference type="OrthoDB" id="3266505at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF3">
        <property type="entry name" value="HALOTOLERANCE PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="20"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="83"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="96"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="681" mass="77584" checksum="B90547744F239DF8" modified="2011-11-16" version="1">MSGNIQKPKPLKVFGRSHYACTRCKVSKIKCSGERPACANCKSINKGDACVYPTKDRKIVIMESDLNKLHDRVEYLESIIASSNPSHTTEPERAPLNTTTVGTSASHSYPSMSTIGSTKKIPVSGNVINGIELVNFYAPEFDDYDLQYNLLLACQNKLPERTYALNLVNKVYFTYNQEFYLLAIQEFLDLTNDIYALFDGLQQEQQQQTSGQPQTHQNSEPPLRSITQISLCYFFILIAFGEQLLNVKTDENKYPGMEYYLLAEHLFRLTRSDLDITFMQSSLLLGLYSVNLARYNIAYNYLGIAARSAVAQGYHRQRDIPGNKSPEEYRELRIFAERSKRLWWTIFVIDSLWAAKTENFQYTDTDVDLPTENTFDLGDSFDGTILETNVHLTKYVTKFVRLIYGPNIRTFSVNYINTNQFNQKLLLKNILTSSSDLIKNFEFPLLSKFRGIDYVNDGSRMLANLLLRYHQLNILLTKPLLSLATDPSSSELLENYHDIEATIAKGMFVSCSTIDIVARLYTCQKIFVLGYWDSHHLFSSLMMLIIAGLRGKVYPQVNKAIALLKYLAGMGNINANTYVKKLNDISYELQCSGIAFKLDLSVTVDTIDFDKNDYYKNPPSTKPQSNYLFEARTAVPYLPEAYADTIEGINTFLSSSHGISINDKSRDFYMSIINHIQGWEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFK9</accession>
    <name>G3AFK9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_58120</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372410.1">
        <property type="nucleotide sequence ID" value="XM_007372348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFK9"/>
    <dbReference type="FunCoup" id="G3AFK9">
        <property type="interactions" value="729"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFK9"/>
    <dbReference type="GeneID" id="18872331"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58120"/>
    <dbReference type="eggNOG" id="KOG0126">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045495_2_2_1"/>
    <dbReference type="InParanoid" id="G3AFK9"/>
    <dbReference type="OMA" id="PMEQFIK"/>
    <dbReference type="OrthoDB" id="2573941at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051847">
        <property type="entry name" value="RNA_proc/Spliceosome_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45880">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN, X-LINKED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45880:SF1">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN, X-LINKED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="33"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="157"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="182"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="197"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="218" mass="25620" checksum="4B5C4705FE5956F8" modified="2011-11-16" version="1">MNSIHKINQINQKELATNTSYKSSWHYDYRDTNYIYIGNIPSSLTAQDIVIIFSQYGIPTHLNLVKDKTTGEHRGFAFLKYAHFKSCILAIDNFNGIKVGDRNLRVDHNYYKLKGGELESDYLVDYSEIQGVKQQLENKDTKLIENKEADELKDPMEKYIDDDLRDPIENYKEAGNDDDELKDPMEAYLKRSDKHDKHGRHRNRHRSGHKDRERSPKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG32</accession>
    <name>G3AG32_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_58370</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372583.1">
        <property type="nucleotide sequence ID" value="XM_007372521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG32"/>
    <dbReference type="GeneID" id="18872419"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58370"/>
    <dbReference type="eggNOG" id="ENOG502QWIS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1435247_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG32"/>
    <dbReference type="OrthoDB" id="415590at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF46">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="12"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="189" mass="22106" checksum="2BFAA87CE37EB763" modified="2011-11-16" version="1">MDTPKKTRSRTGCFTCRKRKKKCDETSYPVCHNCQAKGLECNWPPLTHEIHKKIEQVKYVDQDRRGYKTIDREEEYKSLDEESDIIIEKSIRHDKEVVPRIHSGENIKISKPWKNNNKYYLQRIAMQQDIVDEQQPSAYDSKVPYNSETLTYSPTVDPGDSTLSPFIEDPFLDLNFNRMNDVTGTTPDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG51</accession>
    <name>G3AG51_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EXS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_58386</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372602.1">
        <property type="nucleotide sequence ID" value="XM_007372540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG51"/>
    <dbReference type="FunCoup" id="G3AG51">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG51"/>
    <dbReference type="GeneID" id="18872430"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58386"/>
    <dbReference type="eggNOG" id="KOG1162">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048591_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG51"/>
    <dbReference type="OMA" id="RRWLWCF"/>
    <dbReference type="OrthoDB" id="2159384at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF46">
        <property type="entry name" value="PROTEIN ERD1 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="6">
        <location>
            <begin position="198"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="424" mass="50307" checksum="AAED15B40EA34F7C" modified="2011-11-16" version="1">MPEPGNTFLFNDLIPLPFRILFLIQLGATFWYLLVVILYYLSPLNVLQLINLSYSNHNYAELDNIVPSTGEFATTVPADREENTRLIRGIYNDLKGISIVNLICWAAFKVLQRYEWLKYFSYPIPAIAFAFLFYRVFYKSTNTMGQVRIFTTIKRILIGNINSISMRTNDILISDSLVSYAKVLNDFSLFMWTYYYAPNQDYDYKLEMFVLAIPTFIRIKQCYNEIQITGQRSHLFNLIKYSTNLGPLIVNALIKRTLINSTDAERKSGELLNQLNTLNNYWYFFSALNSTYSFIWDVKMDWSLGLFDFIFNINHPYKFQPLRRNLAYKKPVYYLAIVVDFMLRFIWLLKIFIIKEELEHKVTYLHLFSTFLFGYDAYSFGYTIVELLEIYRRWLWCFLKLESDWCKLSVEKVEDIELSQIKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG73</accession>
    <name>G3AG73_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Member of Set1p complex, histone methyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_58412</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat SWD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372624.1">
        <property type="nucleotide sequence ID" value="XM_007372562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG73"/>
    <dbReference type="FunCoup" id="G3AG73">
        <property type="interactions" value="1071"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG73"/>
    <dbReference type="GeneID" id="18872442"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58412"/>
    <dbReference type="eggNOG" id="KOG1446">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044117_3_0_1"/>
    <dbReference type="InParanoid" id="G3AG73"/>
    <dbReference type="OMA" id="KICVLNG"/>
    <dbReference type="OrthoDB" id="27537at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042800">
        <property type="term" value="F:histone H3K4 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031126">
        <property type="term" value="P:sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037867">
        <property type="entry name" value="Swd2/WDR82"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861">
        <property type="entry name" value="WD40 REPEAT PROTEIN SWD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="126"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="361" mass="39342" checksum="784CB857CFFAC5F2" modified="2011-11-16" version="1">MSVNAVNSKFIASSSSTVQVTEKVLASCRPAKLFDYHQGATISSLDFDDSGQYLISSGIDKSIQLYDCHKGVHKGDIQSQKYGAHSARFTHSDLNCLYASTPTEPQENEHAIRYLSLSNNQYLRYFKGHKRQVSSIEVNPIHDTFISSSFDGTVKLWDLKSSSPTGNIEVGQNSVVGFDPQGVVFAIGKYPGAESSTGILSIYDLKSFDKGPFLQVEIPTLQGQLWNSIEFSNNGKHILISTDSCQHYIVDSFSGHVQAIIQLASPTPNKWMSFKYPYTGCCTFSPCGKFVLAGSPKSVVHIFDISNLKSAKGAPTDPVILTNSITKLNSTTGIPKILAFNPKLLTFATADTTVTLWSPSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGA2</accession>
    <name>G3AGA2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sphingoid long-chain base transporter RSB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_69538</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the ATP-dependent translocation of sphingoid long-chain bases (LCBs) from the cytoplasmic site toward the extracytoplasmic side of the membrane (flip-flop). Involved in the establishment of the functional lipid asymmetry of the plasma membrane. Regulates intracellular levels of LCBs, sphingolipid precursors that are growth inhibitory at increased levels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lipid-translocating exporter (LTE) (TC 9.A.26.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372651.1">
        <property type="nucleotide sequence ID" value="XM_007372589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGA2"/>
    <dbReference type="FunCoup" id="G3AGA2">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="GeneID" id="18875277"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_69538"/>
    <dbReference type="eggNOG" id="ENOG502QU4U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033465_6_3_1"/>
    <dbReference type="InParanoid" id="G3AGA2"/>
    <dbReference type="OMA" id="YLCQIIC"/>
    <dbReference type="OrthoDB" id="3358017at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007568">
        <property type="entry name" value="RTA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465:SF9">
        <property type="entry name" value="SPHINGOID LONG-CHAIN BASE TRANSPORTER RSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04479">
        <property type="entry name" value="RTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="108"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="214"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="328"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="429" mass="49111" checksum="538C94666ECC41A1" modified="2011-11-16" version="1">MGFGAENITSWYPKTLPTSTTISTIDPTNSAGLYETLLEISIRAQTETNVVKLYYLSRQFRGAAASLTIISQQQYLATATATQYFPQITEEMFNATLNLKSLHWESNIYATVLSLPGNGIFAILFLALMIFFSIIFCKSRLRYFGMCMSCASALEFIGYLARFIAHFNWSNKDLFLCQIITLTVAPTFTMAGIYFLLAQFIVLHGRKYSVLRPLWFSFVFLIGDLVSLLVQGGGGIYAAVAISQLQNTRPGTWIMVGGLGFQVGCMTLFLVLYIDFINRLYFRANPTIKFSIRRTFQLLFNTLEGRLLRQELEPYYEPSLAFIRKRSFFYYLPLVILISSIFIYIRCIYRMIELSQGWRGYLITHEGFVLTLDAMMVFLACSLYVPFHPRHIFGKDISISNSLEIPMKKKRLKKMKTEVQLWSSMQAGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGA6</accession>
    <name>G3AGA6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_58458</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372657.1">
        <property type="nucleotide sequence ID" value="XM_007372595.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGA6"/>
    <dbReference type="STRING" id="619300.G3AGA6"/>
    <dbReference type="GeneID" id="18872457"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58458"/>
    <dbReference type="eggNOG" id="ENOG502S997">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057545_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGA6"/>
    <dbReference type="OMA" id="QYNYNRR"/>
    <dbReference type="OrthoDB" id="2152896at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="167"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="229"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="267"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="286"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="309"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="327" mass="37108" checksum="9A626EC7889C5C5A" modified="2011-11-16" version="1" fragment="single">MTASTQYNYNRRNSTTSSSPYTIPAKHQQVHVQHMPSSYQNHHHHHSHHYQQQPSQYAPQQQQIHHQQQFAQNTAPLSSSVPTLTREFVVRRISEGETGRLKEELRCEACGKGYKHISSLAKHLWEHTPEWNVTKKLLISKHQQVQLLEAASILVGMNEPAGTTINTRQHSVHQRHPSSDDPKYSSPFSPPFPSNDNSSNTPTPPLNEPSPKFKNGYQTDEIFRGASDSNLKFDRSHSVSQYPPSINGHRREPSSPALNGGYLTKPTLKEEDNEHDHDQEHGLKSPSLSSSFANGSDFQAPPVRTKFAQIEHDEHDENDDNKKDESF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGD7</accession>
    <name>G3AGD7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_58495</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the V-ATPase e1/e2 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372688.1">
        <property type="nucleotide sequence ID" value="XM_007372626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGD7"/>
    <dbReference type="FunCoup" id="G3AGD7">
        <property type="interactions" value="87"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGD7"/>
    <dbReference type="GeneID" id="18872471"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58495"/>
    <dbReference type="eggNOG" id="ENOG502S76V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170555_1_0_1"/>
    <dbReference type="InParanoid" id="G3AGD7"/>
    <dbReference type="OMA" id="WAITYLC"/>
    <dbReference type="OrthoDB" id="1508846at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008389">
        <property type="entry name" value="ATPase_V0-cplx_e1/e2_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12263:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12263">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05493">
        <property type="entry name" value="ATP_synt_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="71" mass="8041" checksum="EC5B65A49B27546C" modified="2011-11-16" version="1">MSGYTVLIVFIITVGLSCCAWVFAPKENTTVFRSSVILGMAMCYLMWAITYLAQLHPLEAPRRSDLRPEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGI5</accession>
    <name>G3AGI5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Monopolar spindle protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_132013</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372736.1">
        <property type="nucleotide sequence ID" value="XM_007372674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGI5"/>
    <dbReference type="STRING" id="619300.G3AGI5"/>
    <dbReference type="GeneID" id="18869629"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132013"/>
    <dbReference type="eggNOG" id="ENOG502RQ02">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_771585_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGI5"/>
    <dbReference type="OMA" id="MFALNIM"/>
    <dbReference type="OrthoDB" id="4007944at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="320"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="116"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="174"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="232"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="397" mass="46410" checksum="42C54BFFAF7F6747" modified="2011-11-16" version="1">MAIGISRPVDDLINLSWIRTARGVNREYIYASQLQSVIAEIEKALAIGSILTEDEMRLFIKLAEKKQDMKIKKTQLKELISAAAEMHSFEELLQTRFNMGESDIRRCLNVPSSGYDEHTKSPIVPVSRPLSSRTPSDERSGAKSDKGLLWEELHVKDKYIHDKDHEIELKNTENLNLRRINREQEKSIRKLDNELSVLKNHVRSLESQLSKIPGERATRNLLTKLKDRDLVIKSLEQVCEEYRENISDLEKKEAENFKELNEIKKTLNHQDQIFENLQKNLPVGSTGDTKLKGFLMNLPFVKQYLYYLQYKHENKDVKMFILNIIALIFAACLVGNILQLIFYVIMAFIRSKGECETAQYVYDDYNFNGGWLTRSSFFDNWRGIMWLEELIYRFTDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGR6</accession>
    <name>G3AGR6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription initiation factor IIA large subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_58617</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIA subunit 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372811.1">
        <property type="nucleotide sequence ID" value="XM_007372749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGR6"/>
    <dbReference type="FunCoup" id="G3AGR6">
        <property type="interactions" value="475"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGR6"/>
    <dbReference type="GeneID" id="18872535"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58617"/>
    <dbReference type="eggNOG" id="KOG2652">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030027_2_1_1"/>
    <dbReference type="InParanoid" id="G3AGR6"/>
    <dbReference type="OMA" id="QKCTGEA"/>
    <dbReference type="OrthoDB" id="6275927at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07976">
        <property type="entry name" value="TFIIA_alpha_beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.18.10:FF:000006">
        <property type="entry name" value="Transcription factor TFIIA complex subunit Toa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.100:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004855">
        <property type="entry name" value="TFIIA_asu/bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03153">
        <property type="entry name" value="TFIIA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01371">
        <property type="entry name" value="TFIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47396">
        <property type="entry name" value="Transcription factor IIA (TFIIA), alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50784">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="58"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="177"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="72"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="185"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="276" mass="31062" checksum="13EB47A74869DFE5" modified="2011-11-16" version="1">MSNAEASKLYETIIEDVINDSRQDFENNAIDESTLQELRKIWCEKLSQTGVAKFSWDEEYEEHHDEDHQDDVQSNTNGTASNNVLDGLGNVSDLGDVPTTTDIANNGSELELPDVQLSYNTTTSMGIELPVMSKKEEDDSGLILPRINQADGTFEFTIYTDSADKFINTLKKKNKKTKIPQVDGDIGEDVDLGNDDDDDDGDMFNDSDDINSDLDDEFDSERSDEEDGDQEGQIMLCLYDKVQRIKNKWKSNLKEGIANIDGKDYVFHKATGESEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGS2</accession>
    <name>G3AGS2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Multicopy suppressor of BFA-induced lethality ER-golgi vesicle-tethering protein p115</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_132430</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372817.1">
        <property type="nucleotide sequence ID" value="XM_007372755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGS2"/>
    <dbReference type="FunCoup" id="G3AGS2">
        <property type="interactions" value="217"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGS2"/>
    <dbReference type="GeneID" id="18869658"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132430"/>
    <dbReference type="eggNOG" id="ENOG502QRKP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023943_2_0_1"/>
    <dbReference type="InParanoid" id="G3AGS2"/>
    <dbReference type="OMA" id="AHWKEDQ"/>
    <dbReference type="OrthoDB" id="2195113at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042175">
        <property type="term" value="C:nuclear outer membrane-endoplasmic reticulum membrane network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039604">
        <property type="entry name" value="Bfr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31027:SF2">
        <property type="entry name" value="LEBERCILIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31027">
        <property type="entry name" value="NUCLEAR SEGREGATION PROTEIN BFR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="431"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="21"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="160"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="251"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="432"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="459" mass="52255" checksum="0624B098AA770A22" modified="2011-11-16" version="1">MSSSPVPFKSAPKKFIKRPDDKALKAEIDELKNEIKKLDLSNNDLNGQISKLQIDQSVMDKRNKLQAELKGLIAKQANVKNERNAINDQIKAVDAQLKKRIAEIQAQTSKNSFKNVEEIDARIKYLDGLIDAGDLKLADERRFVKEMSGLRKLRKDFGGIEKIQALIDQDKEKIAELKKKLSGVQNKEIQAQFEAIQKELDEINESNKTIISQRNELYDKRAAVKKQKDEKYDQIRKLRVEFDEKFANFKKQLAEEKKKRDEEYKAKLLEEKQQKRKEFAQQQLAEASIPAFTAEINSIHNLLAHFDPTYVKPAPKKASNGESSTLPTNDNIRKVEFPEDLVVIKKEQEDFFTGSKSKKSAKHKKKSKNFNVAPDVVVALSDLSIPFPTKEEEVEPTVKILKETLVALEEKQEEQTKTNIEKAKARIAKLEAEEDAAEAAEEAETATPEVEVEAEEVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGV0</accession>
    <name>G3AGV0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Component of chaperonin-containing T-complex</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_57670</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372035.1">
        <property type="nucleotide sequence ID" value="XM_007371973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGV0"/>
    <dbReference type="FunCoup" id="G3AGV0">
        <property type="interactions" value="1580"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGV0"/>
    <dbReference type="GeneID" id="18872143"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57670"/>
    <dbReference type="eggNOG" id="KOG0362">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_4_2_1"/>
    <dbReference type="InParanoid" id="G3AGV0"/>
    <dbReference type="OMA" id="WGLKYAV"/>
    <dbReference type="OrthoDB" id="1748577at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03341">
        <property type="entry name" value="TCP1_theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000008">
        <property type="entry name" value="T-complex protein 1 subunit theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012721">
        <property type="entry name" value="Chap_CCT_theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02346">
        <property type="entry name" value="chap_CCT_theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="550" mass="59992" checksum="1AE845F14C298C5E" modified="2011-11-16" version="1">MSLRLPQAPNSGLFKQGYQSFSNSDGAIIRNIEAVREISSMLVTSMGPSGRNKIIVNKLGKKFITNDAATICNELEIVHPVVKILIMASKQQEFEMGDFTNLVIILAGEFLNVAEKLLLLGLNVTEIIQGFSLANKYVMKILDELVVDKVESIMQKEHLLKVVKPVIAAKQYGYEDLISNLVVEAVQSVINPKNPASFNIDSIRVVKIMGSSLPQSQVVKGMVFPREPEGSVKHIKSKSKVVVFTCPIDISTTETKGTVLLHNAQEMLDFTKGEEQQLDQVVKEISASGVKVVVAGSNVGELALHYFNKYDILLLKVGSKFDLRRLCQVCGATPLPRLGAPMPDEMGEIDIIETKEIGGDRVTIFRQAESSVSRTATIVIRGATQNNLDDIERAIDDGVNSIKGLLKDDRLLPGAGAIEIELIKRITQYGETTPGLLQLAIKNFAKAFEVIPRVLIETSGFDSTEVLAKLHAAHSEEPGLNVGINIDDGELVDISTIGIYDLLSSKKSAIDLAVEATNTILSIDQIIMAKRAGGPVMPQQPRPGNWDQDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH56</accession>
    <name>G3AH56_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">COP9 signalosome complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_132677</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Component of the COP9 signalosome (CSN) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M67A family. CSN5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372898.1">
        <property type="nucleotide sequence ID" value="XM_007372836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH56"/>
    <dbReference type="STRING" id="619300.G3AH56"/>
    <dbReference type="MEROPS" id="M67.A13"/>
    <dbReference type="GeneID" id="18869680"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132677"/>
    <dbReference type="eggNOG" id="KOG1554">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053034_1_0_1"/>
    <dbReference type="InParanoid" id="G3AH56"/>
    <dbReference type="OMA" id="VKMKLFQ"/>
    <dbReference type="OrthoDB" id="605656at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019784">
        <property type="term" value="F:deNEDDylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08069">
        <property type="entry name" value="MPN_RPN11_CSN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000203">
        <property type="entry name" value="COP9 signalosome complex subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0736">Signalosome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="14">
        <location>
            <begin position="70"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW35486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="261" mass="28946" checksum="DD33D888511AEF23" modified="2011-11-16" version="1" fragment="single">MPCFHDLATALQSNNNAKIKGTKIVDLYDLGITDKPRTSNATKFYNIPPVAPALAKSRPWKADPHYFSTCYISSLALVKMTIHAQSGGNIEVMGMLIGKVISGAIIIMDVYALPVEGTETRVNAQAEAYEYMVQYLEMNKKITGRNENIVGWYHSHPGYGCWLSGIDVSTQSLNQGFQDPYLAIVVDPIKTVKQGKVEIGAFRTFPENYVPTSDGSHLSSKPAVNIPKAKRKDFGSHFDKYYPLDIEIFSSDVDESIIQML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHA2</accession>
    <name>G3AHA2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ureidoglycolate lyase</fullName>
            <ecNumber evidence="3">4.3.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Ureidoglycolatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_131197</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the catabolism of the allantoin degradation intermediate (S)-ureidoglycolate, generating urea and glyoxylate. Involved in the utilization of allantoin as secondary nitrogen source when primary sources are limiting.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(S)-ureidoglycolate = urea + glyoxylate</text>
            <dbReference type="Rhea" id="RHEA:11304"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57296"/>
            <dbReference type="EC" id="4.3.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nitrogen metabolism; (S)-allantoin degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ureidoglycolate lyase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.3" evidence="3"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372945.1">
        <property type="nucleotide sequence ID" value="XM_007372883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHA2"/>
    <dbReference type="FunCoup" id="G3AHA2">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHA2"/>
    <dbReference type="GeneID" id="18869558"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131197"/>
    <dbReference type="eggNOG" id="ENOG502S1JQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070848_0_1_1"/>
    <dbReference type="InParanoid" id="G3AHA2"/>
    <dbReference type="OMA" id="ECYFEPG"/>
    <dbReference type="OrthoDB" id="10261384at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004848">
        <property type="term" value="F:ureidoglycolate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050385">
        <property type="term" value="F:ureidoglycolate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000256">
        <property type="term" value="P:allantoin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20298">
        <property type="entry name" value="cupin_UAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.480:FF:000003">
        <property type="entry name" value="Ureidoglycolate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.480">
        <property type="entry name" value="Ureidoglycolate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047233">
        <property type="entry name" value="UAH_cupin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007247">
        <property type="entry name" value="Ureidogly_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024060">
        <property type="entry name" value="Ureidoglycolate_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21221">
        <property type="entry name" value="UREIDOGLYCOLATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21221:SF1">
        <property type="entry name" value="UREIDOGLYCOLATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04115">
        <property type="entry name" value="Ureidogly_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW35533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="201" mass="22458" checksum="FAA2BFA3E6345C9A" modified="2011-11-16" version="1">MVLETFDGSKIPPIIAKLLTPENFEPFGGVISPEHQVIANDSNANYGTAIKIPKVAPVVNNYKMSQSGSSTTNWNIFRCSRPDHLLTEGRGNWQYTSTVLERHPFTTQTFLPLGKTDEICYLVIVAKSDTKTTLPDPDTIAAFLCRGNQSVTYGVGVWHAPMITIQDRTVDFAVLVHESGIAEEDTQECYYDPGYQVYYRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHG8</accession>
    <name>G3AHG8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">[acyl-carrier-protein] S-malonyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_59550</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>holo-[ACP] + malonyl-CoA = malonyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.1.39" evidence="1"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373716.1">
        <property type="nucleotide sequence ID" value="XM_007373654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHG8"/>
    <dbReference type="FunCoup" id="G3AHG8">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHG8"/>
    <dbReference type="GeneID" id="18872969"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59550"/>
    <dbReference type="eggNOG" id="KOG1202">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030558_0_1_1"/>
    <dbReference type="InParanoid" id="G3AHG8"/>
    <dbReference type="OMA" id="AFHTPFF"/>
    <dbReference type="OrthoDB" id="541883at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.250">
        <property type="entry name" value="Malonyl-CoA ACP transacylase, ACP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050858">
        <property type="entry name" value="Mal-CoA-ACP_Trans/PKS_FabD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42681">
        <property type="entry name" value="MALONYL-COA-ACYL CARRIER PROTEIN TRANSACYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42681:SF1">
        <property type="entry name" value="MALONYL-COA-ACYL CARRIER PROTEIN TRANSACYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Malonyl-CoA:ACP transacylase (MAT)" evidence="5">
        <location>
            <begin position="14"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="321" mass="35260" checksum="68AB99B51F8D6C2B" modified="2011-11-16" version="1">MNHTIARTVKYALTCPGQGISANGLLQAYKPFQPEFQKYLEEIDATLNCNFSKHLFADEDTARAQQWFGKTSNAQPAILSATYILTQLLKSRGVDVVGSAQYLMGHSLGEYTALCLSGIVDFGTAVKIVRRRGELMEEIAENSGVKYGMSALLISPANYEQVSELARESGVLANINSPHQLVIAGEEKHIGEFIEKCGRKLVRRSVKLPVSIPFHTSILKPMSQELADMFGNINPQEKPIVSNFTGEVSTDAATTVDNTVKANYQPVQWLKSMSLLQKENVTHVVNLGPGTALHGINQKYDNIVSVSVDSFDNLDELNELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHJ8</accession>
    <name>G3AHJ8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein FMP42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_59592</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC43A transporter (TC 2.A.1.44) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373746.1">
        <property type="nucleotide sequence ID" value="XM_007373684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHJ8"/>
    <dbReference type="GeneID" id="18872979"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59592"/>
    <dbReference type="eggNOG" id="ENOG502QRYG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014401_1_1_1"/>
    <dbReference type="InParanoid" id="G3AHJ8"/>
    <dbReference type="OMA" id="CNILQQV"/>
    <dbReference type="OrthoDB" id="330047at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052599">
        <property type="entry name" value="SLC43A_AATransporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20772">
        <property type="entry name" value="PROTEIN FMP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20772:SF2">
        <property type="entry name" value="PROTEIN FMP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="366"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="420"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="450"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="489"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="532" mass="58411" checksum="162CDDC117D0CB40" modified="2011-11-16" version="1">MRGLGYRPSTSRRLLQISCAIGWCLFAAGPIFGFAAIKPIFIKEGVYHDKCPANSNSECTQQELALDFLFTAGCMVTNISALPVGWILDSYGPKTTGIIGSFILGIGAICLSLASNILFMDGYLAGFILLALGGPFVFISCFQLANSFPKRSGLVLALITGSFDSSSALFLFYRMYYDNIHKLSVSGFFACYLIVPIFIYLCQILLMPEELYKTVATLAKIAETGIDETGKPLDTDLLYPEDIAEDSDDNTVEYYQPTIQETTSLLLRRASISAQVPSIRRASFSSVASNKSVYEQEADLKLTRSSGGVFGILHGYSIRDQLSSSWFLLMCLFTMTQMLRINYFVATIKSQELYLYNGNEELATSINHFFDIALPVGGIIAVPFIGMILDNCTTLSVLALLTGLTVFVGIAGLLSWLPATYAGILVLVVFRPFFYTSVSDFCAKVFGYETFGTVYGSIIAFSGLCNIFQQVMDKVTLEIFKKNPGPINIILTVLTGVSGFALIGFVKSQEMEIKRLQLEMEAQEAIDRPIPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHS8</accession>
    <name>G3AHS8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59671</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373826.1">
        <property type="nucleotide sequence ID" value="XM_007373764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHS8"/>
    <dbReference type="FunCoup" id="G3AHS8">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="GeneID" id="18873016"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59671"/>
    <dbReference type="eggNOG" id="ENOG502S6BB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125085_1_0_1"/>
    <dbReference type="InParanoid" id="G3AHS8"/>
    <dbReference type="OMA" id="ISKPIEC"/>
    <dbReference type="OrthoDB" id="4069967at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16588">
        <property type="entry name" value="zf-C2H2_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="167" mass="18986" checksum="1700F9EA64E69671" modified="2011-11-16" version="1">MSIVELSATVDKLAKIINQKNKQLEDLKQGYDQKLHIINEYIRTLEGSIEGDSSKESIPNEEFIKKISSKVECPNCKHVIWDNMQSGEYVLVPKHQLKYLIRDTPQAQTQAHEPITPTTSTSNDTSYRPPKPKNRSKINCSFCGEPGHTRAKCYKRLTTPKEETGNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI10</accession>
    <name>G3AI10_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_149038</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373907.1">
        <property type="nucleotide sequence ID" value="XM_007373845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI10"/>
    <dbReference type="STRING" id="619300.G3AI10"/>
    <dbReference type="GeneID" id="18870809"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_149038"/>
    <dbReference type="eggNOG" id="ENOG502RQR0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032656_0_0_1"/>
    <dbReference type="InParanoid" id="G3AI10"/>
    <dbReference type="OMA" id="HYYETTI"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF2">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="507" mass="58119" checksum="AF236EBFD97B52B1" modified="2011-11-16" version="1">MNEQIIQPIISQYYHSNDGHGHSFIYGPINDLDSIAGSLHPIVENPRTFKPPKPKENTRPRINKLTTPQENSTRLFKHPITDIGEYSIDELKQLFLNHLHMIEPTNKLSSFSANLKNEIEYQMFNYFVNNLSSQLDVFFEVKVYSEIVSQMALYDDSGILLQSVFCLSSLMLHRMMPDKIDASLPIKYYHHSIKTIKSYLDMFNHDMGVCSKCLLATTLLSVYEFYFTSIEDIYTGNAASILASIIQSDNSRNKSSPLLDSVFLATCFWSVFTLGFIQSLKNVRVNIYSVDLFWKALDSEYFTVFDKYMVLTEASEPKTTLSKTETVWWFRKTILICSQISDFKGEVNVPTQQDVEQNQSFKKWIELKDILVDFENKAPDGLFPVIYHSNSDSGAFPTIFFKDELSTLIGINRALAKLLLFESLLVKTNIQAREVRDELSKFPPGYEKKLAKDLIGIIKCYDSNSTIWPLNLHVLGYVQAYLADEEQAINEMSPLITKLSKVCNLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIC6</accession>
    <name>G3AIC6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein BFR2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_147617</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AATF family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373279.1">
        <property type="nucleotide sequence ID" value="XM_007373217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIC6"/>
    <dbReference type="FunCoup" id="G3AIC6">
        <property type="interactions" value="959"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIC6"/>
    <dbReference type="GeneID" id="18870760"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_147617"/>
    <dbReference type="eggNOG" id="KOG2773">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018299_2_2_1"/>
    <dbReference type="InParanoid" id="G3AIC6"/>
    <dbReference type="OMA" id="INFMAPN"/>
    <dbReference type="OrthoDB" id="5783963at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025160">
        <property type="entry name" value="AATF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039223">
        <property type="entry name" value="AATF/Bfr2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012617">
        <property type="entry name" value="AATF_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15565">
        <property type="entry name" value="AATF PROTEIN APOPTOSIS ANTAGONIZING TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15565:SF0">
        <property type="entry name" value="PROTEIN AATF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13339">
        <property type="entry name" value="AATF-Che1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08164">
        <property type="entry name" value="TRAUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AATF leucine zipper-containing" evidence="5">
        <location>
            <begin position="182"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Apoptosis-antagonizing transcription factor C-terminal" evidence="4">
        <location>
            <begin position="367"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="452"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="16"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="32"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="81"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="453"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW33695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="481" mass="55132" checksum="E0481D03C889218C" modified="2011-11-16" version="1">MAKKSLAEQIAGLFKPKTDFDIEDHEQKEVFEQQEENSDVDEEDEEEANDHYIRNEKSKLRNDAPGLGEKYVGNVVSRDQLEEEEEEEEDDDIEEEQSEDEDDDIEEEQSEDEDDNQEDEQDEEEVNEEEYSLDGSSEDSEEEDSEGSDEEASSNKRQLLKKLMSQERTHLLSRLSQSATTDAIKGYAINTQHSTFDKIIDARLKFQKSITTANLFPTSDISSYTTEHTPKLIHQTKEELYTLLDSIIALRSELEGKEYTAPKKRTYSAYCQTASEADKSLASTRASVLTKWSAKVQNSSGASAINATKFKTINQSFETQVENNLSDMGRLVKRTKLNRRQVRPMGHTEEEEVKQEVETMFDDEDFYRVLLNDLVDKKVQSNGSIAIGLRSAQRANKLKNNVDTKASKGRKLRYHVQEQIANFETSVGGWRWNDDQIDEFFASLLGQKVNMNEQDESEEEHEEEEEEEEVIPQDSGITLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIF7</accession>
    <name>G3AIF7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">U three protein 23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_135247</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in rRNA-processing and ribosome biogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the UTP23/FCF1 family. UTP23 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374011.1">
        <property type="nucleotide sequence ID" value="XM_007373949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIF7"/>
    <dbReference type="FunCoup" id="G3AIF7">
        <property type="interactions" value="1095"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIF7"/>
    <dbReference type="GeneID" id="18869878"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135247"/>
    <dbReference type="eggNOG" id="KOG3164">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053567_1_1_1"/>
    <dbReference type="InParanoid" id="G3AIF7"/>
    <dbReference type="OMA" id="CCMQALY"/>
    <dbReference type="OrthoDB" id="25675at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09865">
        <property type="entry name" value="PIN_ScUtp23p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000006">
        <property type="entry name" value="rRNA-processing protein UTP23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006984">
        <property type="entry name" value="Fcf1/UTP23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057776">
        <property type="entry name" value="UTP23_sensor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04900">
        <property type="entry name" value="Fcf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24779">
        <property type="entry name" value="UTP23_sensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="UTP23 sensor motif region" evidence="9">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="171"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00076388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="271" mass="31000" checksum="043E713152C11889" modified="2011-11-16" version="1">MRQKRAKSYKKQMTIYHHTFKFRSPYQVLLDNELVLNITQASYNLMNGLTKTIQGEIKPMITQCCMQALYDTKNQTAIDLAKTFERRRCNHRDAIDPAECIESVVDIDGVNKHRYVVACQNLALRKKLRKIPGVPLIFMNRSVMVMEPLSDVSAKYSREFEEKKLTAGLNSIESRRKAEEEKGASEEESAKKKRKGPKEPNPLSIKKKKKTEPQPTTESAKKKPNRRRKHKSTTESEANSAKEEDSEAKATNEEAEKPNEPVELSAENESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIW8</accession>
    <name>G3AIW8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_133757</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373363.1">
        <property type="nucleotide sequence ID" value="XM_007373301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIW8"/>
    <dbReference type="GeneID" id="18869764"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133757"/>
    <dbReference type="eggNOG" id="KOG0253">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_52_4_1"/>
    <dbReference type="InParanoid" id="G3AIW8"/>
    <dbReference type="OMA" id="LLWFIWM"/>
    <dbReference type="OrthoDB" id="4139357at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000171">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF12">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G01740)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="332"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="432"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="465"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="493"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="59"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="522" mass="57333" checksum="61CEECC1947C825C" modified="2011-11-16" version="1">MTTPDKQKQAEIAESISEVATFEGELFDEHDADKAYLAKSKLVALAIQEIGFGKYQIGLFFVAGFGWLSDNAWPIATGLIIPRLNEVDGVHAPIGKAPYLTLAQNLGLLMGALIWSLSSDIIGRRWAFNLTFLITGIWAVVAGSSPNFAALGVFCSFWSFGVGGNLPVDSAIFLEALPKSHQWLLTVMSVWWAIGQIIANLISWGLIANFSCASDATICHKADNKGWRYFLFTMGGLTICMFIARFAFNVLESPRYYVARGNDEKAIHTLEKIAKINGKTCPITLADLQEIDIRFSNEDTTKSNKPKNKLVEEKLKKYNLSHIRQCFASKKLAISSALVIFTWGIIGLAFPLYNAFLPYYLETRGNANQPLSVRETYRNTLIVSVLGVPGALIAGFLVELRIGRKGTLFLSLILTGIFLFGSTTAKTSNANLGWNCMFSFVSNIMYGVLYAYTPEVFFSKIRGTAVGLAASFNRVLGVFAPIIAIYADLTTSAPIFVSGALFIFAGLLSIFFPYEPRGKASY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIX0</accession>
    <name>G3AIX0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Lysophospholipase</fullName>
            <ecNumber evidence="2 10">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_70053</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the release of fatty acids from lysophospholipids. Phospholipase B may well contribute to pathogenicity by abetting the fungus in damaging and traversing host cell membranes, processes which likely increase the rapidity of disseminated infection.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the lysophospholipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="2 10"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373365.1">
        <property type="nucleotide sequence ID" value="XM_007373303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIX0"/>
    <dbReference type="FunCoup" id="G3AIX0">
        <property type="interactions" value="106"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIX0"/>
    <dbReference type="GeneID" id="18875303"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70053"/>
    <dbReference type="eggNOG" id="KOG1325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014602_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIX0"/>
    <dbReference type="OMA" id="FARYCWN"/>
    <dbReference type="OrthoDB" id="4084751at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07203">
        <property type="entry name" value="cPLA2_Fungal_PLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000010">
        <property type="entry name" value="Lysophospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF33">
        <property type="entry name" value="LYSOPHOSPHOLIPASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9 10" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6 9 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005131606" description="Lysophospholipase" evidence="10">
        <location>
            <begin position="18"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="PLA2c" evidence="11">
        <location>
            <begin position="30"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW33781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="627" mass="69768" checksum="26EF2EF0D4DD496A" modified="2011-11-16" version="1">MFHLVTLLLFNFCLISAWSPTDSYAPGEIACPKNYNGVRLADQLSNEESDWIQSRNEITDKNLIEFLNHANMDDFDAENFITGLNRSIKIGLAFSGGGYRAMLCGAGQFSALDNRTRGAWEHGLGGLVQASTYMVGLSGGNWMVGTIAMNNFTHVEKIIEQGTIWDLSSSIINPGGWKIWKTASTFKSIDDDLDDKKDAGFELSLTDFWGRSLSSQFFAGLDDFGAALTWSNLQNADVFTNHEMPFPIVVSLGREPGTLIVSINSTLFEFNPYEMGSWDPTLYQFTPVKYLGTEYNNGNPVNDTCIGGFDNAGFIMGTSSTLFNQFLLQINTTSIASTVKSIITTILKRVSHKEDDIAIYRPNPFYNTSAAIDKISKNDTLFLVDGGEDLQNIPLAPLLQPERDVDVIFAFDNSADTEYSWPNGTSMIATFERQYNRQGVNASMFPYVPDSQSFINLNLTSRPAFFGCDSKNLSTIQAQTHNPTSAFSSPLIVYTANRPFSYSSNTSTFKMSYENGERDAIIQNGFEVASRNNMTLDDEWRACVGCAIIRREQERQGQEQSEQCKQCFAKYCWDGSLDRTSQINANFTDNGSTEGHTKIAIRSLCKNYVIKRACSITMFGNKDTGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ62</accession>
    <name>G3AJ62_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Actin-related protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_59180</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the actin family. ARP2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373403.1">
        <property type="nucleotide sequence ID" value="XM_007373341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ62"/>
    <dbReference type="FunCoup" id="G3AJ62">
        <property type="interactions" value="1085"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ62"/>
    <dbReference type="GeneID" id="18872810"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59180"/>
    <dbReference type="eggNOG" id="KOG0677">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027965_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ62"/>
    <dbReference type="OMA" id="WEDMQHL"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051654">
        <property type="term" value="P:establishment of mitochondrion localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10220">
        <property type="entry name" value="ASKHA_NBD_Arp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000050">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000005">
        <property type="entry name" value="Actin-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="391" mass="44294" checksum="A17056D1D2A65359" modified="2011-11-16" version="1">MSAPIVLDQGTGFVKIGRAGTNFPDHTFPSMVGRPILRAEERNVLIPADVEIKDIMCGSEASEVRSLLEINYPMENGIIKNWEDMEHLWDYAFYERMKINPEGQKILLTEPPMNPLKNRETMCEVMFEKYHFGGVYVAIQAVLALYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLTRRLDVAGRDVTRHLINLLFRRGYAFNRTADFETVRQIKEKLCYVSYDLQLDAKLATETTALVESYELPDGRVIKVGSERFEAPECLFQPHLVDVEQPGVGETLFNMIQSADVDIRSTLFKAIVLSGGSSMYPGLPSRLEKELKQLWLTRVLHGDATRLDKFKVRIEDPPRRKHMVFIGGAVLANIMADKDHMWISKQEWEEQGSRVLEKLGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ76</accession>
    <name>G3AJ76_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Agglutinin-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_134426</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ALS family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373417.1">
        <property type="nucleotide sequence ID" value="XM_007373355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ76"/>
    <dbReference type="FunCoup" id="G3AJ76">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ76"/>
    <dbReference type="GeneID" id="18869813"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134426"/>
    <dbReference type="eggNOG" id="ENOG502S1H2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031316_2_0_1"/>
    <dbReference type="InParanoid" id="G3AJ76"/>
    <dbReference type="OMA" id="CANIHAA"/>
    <dbReference type="OrthoDB" id="3981162at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903561">
        <property type="term" value="C:extracellular vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030446">
        <property type="term" value="C:hyphal cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030445">
        <property type="term" value="C:yeast-form cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044403">
        <property type="term" value="P:biological process involved in symbiotic interaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030448">
        <property type="term" value="P:hyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2430">
        <property type="entry name" value="Agglutinin-like protein, N-terminal domain, N2 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008966">
        <property type="entry name" value="Adhesion_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008440">
        <property type="entry name" value="Agglutinin-like_ALS_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024672">
        <property type="entry name" value="Agglutinin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043063">
        <property type="entry name" value="Agglutinin-like_N_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033504">
        <property type="entry name" value="ALS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011252">
        <property type="entry name" value="Fibrogen-bd_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793:SF2">
        <property type="entry name" value="AGGLUTININ-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793">
        <property type="entry name" value="ALPHA-AGGLUTININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05792">
        <property type="entry name" value="Candida_ALS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11766">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01056">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49401">
        <property type="entry name" value="Bacterial adhesins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0134">Cell wall</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 17" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0843">Virulence</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442154" description="Agglutinin-like protein N-terminal domain-containing protein" evidence="17">
        <location>
            <begin position="20"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Agglutinin-like protein N-terminal" evidence="18">
        <location>
            <begin position="53"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="442"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW33833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="482" mass="51443" checksum="7ECE22266CA3D735" modified="2011-11-16" version="1">MILSLVLLQLLATFVSTQAISGVFTEFNSLTWENGGGYPYASPSSPSWMAKLGWKIDGSTMNSGDTFTLDMPCVFKFPTTQTSVDLKVGSTSYATCTFSIADILLSSSQLNCVLLSAVQDSTSATGTLTFPVAFNVGGSASSNDVSCASRFQDGVNTVTFKDGSNTLSTQATFQKGVDSDPNKIIYHARTLPMLNKMQHSLAAGNCASGYSTGTLGIKIINNSGQVDCANIHAAISNSLNDWYLPKSVSSSFSFTTTCSSSGYSISYQNVPAGYRVFIDALVSIVVGGDNTVQYLNKYVCSGSTVNNDNSLNVEWKNYQNNVAGSNGQEVVVITETWTGSTTHLTTKTHNSDDPTITVIVDVPIPTITITTTYIGVSTSYTTHTVTPGETASVIEYEPVHTTTTETSCWESDTNGVTTIIKSSLATDTVLYLTPCTTELPPTTQVTSATTTEFTSDDSLSTSSTEQSSTTKSIKYCKRKRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ89</accession>
    <name>G3AJ89_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CREG-like beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_59207</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373430.1">
        <property type="nucleotide sequence ID" value="XM_007373368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ89"/>
    <dbReference type="STRING" id="619300.G3AJ89"/>
    <dbReference type="GeneID" id="18872820"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59207"/>
    <dbReference type="eggNOG" id="ENOG502RDU8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056802_3_0_1"/>
    <dbReference type="InParanoid" id="G3AJ89"/>
    <dbReference type="OMA" id="ITLSMRW"/>
    <dbReference type="OrthoDB" id="2138282at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055343">
        <property type="entry name" value="CREG_beta-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37273:SF1">
        <property type="entry name" value="ADL397C-AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37273">
        <property type="entry name" value="CHROMOSOME 8, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13883">
        <property type="entry name" value="CREG_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442156" description="CREG-like beta-barrel domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="CREG-like beta-barrel" evidence="3">
        <location>
            <begin position="32"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="226" mass="24791" checksum="F16FBEF29D27ECC5" modified="2011-11-16" version="1">MRLSSLLSLSVLSIGTLGAPLADEQVILKDIPTVDEGARVARTLVNRESLTNINTIKTIKQLDGSVRHLPVSSMEYYADCDSDGDPYWLAIDVGGTNQNIIRGSDYSFTIRVGDHPNTEKVDESYPGSIPASPAGSPRVQLTGRLQKITFTNPFDPKKVALERCFLAKHPDAAVWLPNNIVTPHKSHWTKLVVDGVYMVGGFGDRAYIGEINAELYHSAEIIEENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ98</accession>
    <name>G3AJ98_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_70110</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373438.1">
        <property type="nucleotide sequence ID" value="XM_007373376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ98"/>
    <dbReference type="STRING" id="619300.G3AJ98"/>
    <dbReference type="GeneID" id="18875308"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70110"/>
    <dbReference type="eggNOG" id="KOG1502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_9_2_1"/>
    <dbReference type="InParanoid" id="G3AJ98"/>
    <dbReference type="OMA" id="DPCDEKK"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="13"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="342" mass="38052" checksum="442F87782548D74C" modified="2011-11-16" version="1">MIDMGINTASVMVFVSGANGYIAQHVVKQLLAKGYTVVGSVRSESKGDELKLLIQNEKFSYEVIPTLSDKNAFDAALKKHPEVTVFLHTASPVSFAIEDVENDLVKPAIEGTLNALRSIKEHAPQITRVVITSSAVAMWGFGSHFDGNKVHDELDWSPITYEEGLAGASFGYKSSKKYAELAAHDFMTKEKPNFAITYVHPTYTFGPQAYVVKDKKNLNLSNEIVNQVIKLGKDDEIPEHGNVFADVRDVAQAHIVAFESDEAIGQRLLLASGDFTLDSVAEIINRRFPHSNVPRGDVSRNEEIKKKMIHKYDNSKTRKILGFEFKTLEESIYDMVEQLDNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJE0</accession>
    <name>G3AJE0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_149718</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell septum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the formin homology family. BNI1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374183.1">
        <property type="nucleotide sequence ID" value="XM_007374121.1"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJE0"/>
    <dbReference type="GeneID" id="18870838"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_149718"/>
    <dbReference type="eggNOG" id="KOG1922">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002339_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJE0"/>
    <dbReference type="OMA" id="PWLNAFE"/>
    <dbReference type="OrthoDB" id="1104827at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000142">
        <property type="term" value="C:cellular bud neck contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001411">
        <property type="term" value="C:hyphal tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000920">
        <property type="term" value="P:septum digestion after cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2220:FF:000006">
        <property type="entry name" value="Cytokinesis protein sepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051661">
        <property type="entry name" value="Actin_filament_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010472">
        <property type="entry name" value="FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010473">
        <property type="entry name" value="GTPase-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47102">
        <property type="entry name" value="PROTEIN BNI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47102:SF2">
        <property type="entry name" value="PROTEIN BNI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06367">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06371">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01139">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01140">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="9">
        <location>
            <begin position="24"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="10">
        <location>
            <begin position="873"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="680"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="735"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1298"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1411"/>
            <end position="1438"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="551"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="693"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="735"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="759"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="815"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="838"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="853"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1411"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1423"/>
            <end position="1437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1490" mass="168778" checksum="7E62CE8787E75A9F" modified="2011-11-16" version="1">MHVTSKFNPTATVDSFTTVSNFKHQLPKPEIVDTLFENLLAIRVFPTEAIQALRNQSTQRKWELLLRENETNEQFDLNSLSRQASNRFGMHDPLVSSPHIPQSQFRIPSTEELAPSELLPPPEVLPKTRNVGVTRIVTEISPKRNPSIESSGSASLLSGESEGILGMMNKKFKIKDGTPEWYVMRMMSNKLSIKDFKKLEKKLADNIFSTRQGISWIQGFCNAQGEPALAVILGKINKKSFKSNEEFDKEYIIVKCLKHILDSERKEDLLLDEESSTYPLSIKNKTHVIKSLVFSLISPRLTTRILVTEVMVMLMLYQKKVLLTSACQGLTSLQNSVGDHSKFQPWLNAFEELINKRPTNTTTGSGHSNMKNYCVITLMLINSMVELSSSLKERIQIRRELGDAHIVGILEKLRLMEDKRIDNEIEKYEYYAEEDYNTYMTRHVPSPDIDYVEPEEVIIDEREKVYQKPPSDDKDEEENVSSDIIGEVLSKLTSLKKGNRSSENFHHTLVLIDSLLENVSNRSHNLGTESESSLNITVHKLMDRLSTDDMARRAIAESKSLARELEILKQQKEELELQLGIDKLKTIQLLQDENEIQKSQLSMQNKQISLLQRQIKQLEDDKARGGTRNLTPNHAFSVDSFNDDLGKVSGDAKMSEELRSILKNKTDNVVGYSELKDEGVSHYHVPRPPTPPMMFSSSSSHSMDSDDTRKGHITSSKTIKRGMSLHLLNEQLTGKESVGLDQSSSITTDASDDVTQMSAPPPPPPPPPPPPPPPPPPPPPPPPPPPPPLPPFIQSAPPPPPPPLPDFMKQQSAAAPPPPPPPPPLPSFMDKQSAGASLPPPPPPPMPTFMQGSSDSLSSLNSSTEPQFEPQFIIPTLRPKTKMKQMHWDKLDNIQSTFWDNLEYAPLADRLTEHGVFNEVEKVFAAKTTAMKKKKDMDVGADADKPPQKVTFLSRDMAQQFGINLHMFANISEERLVLKVLRCNSDILENVSILEFFNNESLLEISDSLFRNLAPYSVDYLRHPATKPKKDPNELERADRIFLELCYNLRHYWKARSKALLFSQTYRKDYMDMVRKLNAIDEANNCLRNSDSLQQVLGIIRSVGNFMNDTSKQAMGFKIGTLQRLKFMKDDSNSMNFLHYIEKIIRHSFSEYGCFIDDLNALSFVQNISIEQLESDCEEMSKSIQTITYAIESGKLSNPEHLHPQDKILTAISASIQNSKKKNSLLQAHLKRTMSELKSLMAYYGENFEDSNSKGTFFQKFRVFVDEFKRAHVENIQREEEQKAYEARKQMLENRLAEKERKKKELEEEGASEGESVKDGSYSSNTEEKAVIDSVEESSAVIDLLLMKLKSNAHAHPIKSEREKKQKARRSKALSFYSSVSLEEQIETSSPAVSTSDLTQYESVNSLKRRLTNRRKQHHHQSQTDSEAQSAVSSDITSKADEVMSRAQTMLLQLRNEKQSTDVVEVIEVEDEVERKDTLADSELSKEIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJE2</accession>
    <name>G3AJE2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Enhancer of polycomb-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60034</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374185.1">
        <property type="nucleotide sequence ID" value="XM_007374123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJE2"/>
    <dbReference type="STRING" id="619300.G3AJE2"/>
    <dbReference type="GeneID" id="18873188"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60034"/>
    <dbReference type="eggNOG" id="KOG2261">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_803074_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJE2"/>
    <dbReference type="OrthoDB" id="435275at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024943">
        <property type="entry name" value="Enhancer_polycomb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14898">
        <property type="entry name" value="ENHANCER OF POLYCOMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="346" mass="40661" checksum="F1C0A262B9587C4B" modified="2011-11-16" version="1" fragment="single">MDEIDEQFFNDTLTKHYPKVPNAKKNKSEENTRKCTELEFETICDRLEKTIESRQPFLSMDPSNILSYEELSTYIIDQFKSTVKSNNPYVESSGGTLEYISTTTLKERLSKELNYEPFTTLFDKNPMEQTSTRLVRPIPKLFELFGKPIYEHWKERKIERKGKSIKPMLKFEDPNANEKDNDNDPYICFRRREFRQARKTRRADTLGAERIRLLQKSLHRARDLVLSVSERELMKLESLQSELDIFKLRCEAKNLKRTVGVKGDDYLFFPHKRKKVIKPREEEEEREVKRKERRKGESLHAPVPTVHKDKHVPQPQPVAEGAATSQPYVKLPPSKIPDMELVTVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJT4</accession>
    <name>G3AJT4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_70205</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-5/BimC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373569.1">
        <property type="nucleotide sequence ID" value="XM_007373507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJT4"/>
    <dbReference type="FunCoup" id="G3AJT4">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJT4"/>
    <dbReference type="GeneID" id="18875310"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70205"/>
    <dbReference type="eggNOG" id="KOG0243">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001485_33_3_1"/>
    <dbReference type="InParanoid" id="G3AJT4"/>
    <dbReference type="OMA" id="NNDICER"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000073">
        <property type="term" value="P:initial mitotic spindle pole body separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000019">
        <property type="entry name" value="Kinesin-like protein KIN-5D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047149">
        <property type="entry name" value="KIF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970:SF12">
        <property type="entry name" value="KINESIN FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 9 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="3"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="794"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="411"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="794"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="110"/>
            <end position="117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW33985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="865" mass="97746" checksum="4D135AC1D9EB1C24" modified="2011-11-16" version="1">MSNIQVVVRCRSRNQREVDARSPIVVEIPNDRCSIDDPYITVNYNHITTTNTNTSSSTGSSSPLLSNQRTYKVDQVYGSQADQSLVFKNVALPLFNEFFNGFNVTILAYGQTGTGKTFTMCGNEPDIDKLEECDENIGIIPRVLHELFSKLKDSDCDYVVKCSYLELYNEDLKDLLQEDARPLKIYEQNGKSGKSIKIQNLCERQITDYKQGFKYLKMGLDKRKTAATKLNDVSSRSHTIFTINLYKNHGEQDLSGDSRYHIAKMNLVDLAGSENVNRSGSIVKETGSINQSLLTLGRVINTLSEQKPNQHIPYRESKLTRLLQDSIGGNTKTTLIATISPAKVNIDETCSTLDYASKAKNIKNIPQIGHESDSIMKKTLVKNLSVEIVRLNSDLVATRSKNGIYLDPENYQDLVQENESLKTSLKESQLANESIRFKLQEVKESSKVEIDKIKSRMNKLDGKNKQLSTDNHALLSETQKMSIENEQLRQKLNRVSVQMTHSSDKLSKVLFENLNSSITTIKDTITKDSQKHNIDDLIQAQLQLTSQLEKYNHDIDLKVKASNDKVRSLVNDGLSNILDNYRLLSDKISTNQREYIISLQQQISQLKQSNEQLATYLSTDRLALLSNNLSEQITGITESVFTEFKNTIIKSIDDNKNKLCNSQLQAVRGNVDKESQILQQHMMSWRQELSNIFPSIEKEVGAHDQNVQSSMKSAANFGKQGQERINELLHESNTFSLSDIPQPDITKLETISKNLSTDHDKLVEDLTHVKNNLGEIELFDAKNAFKISPIKIAQSTASPDKKQSTPSRRSPPAQVSRIARASTIVGSVRNRDDTKIPKLNRSKSFDDKENRNVKRRKILQLIDNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK36</accession>
    <name>G3AK36_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_60189</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374362.1">
        <property type="nucleotide sequence ID" value="XM_007374300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK36"/>
    <dbReference type="GeneID" id="18873265"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60189"/>
    <dbReference type="HOGENOM" id="CLU_2265401_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK36"/>
    <dbReference type="OrthoDB" id="10400490at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="103" mass="11431" checksum="C8A451D92859FBA2" modified="2011-11-16" version="1">MEILSPSLPESTLTLLEKTLSYYATISINLVYVFTSDLELVGSLDISKSTPLPLNQCVRMGDALSMVTRSKANNVTELDDIDGTRLICHTIDGFIFLYAIRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK42</accession>
    <name>G3AK42_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_136976</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374368.1">
        <property type="nucleotide sequence ID" value="XM_007374306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK42"/>
    <dbReference type="FunCoup" id="G3AK42">
        <property type="interactions" value="956"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK42"/>
    <dbReference type="GeneID" id="18870006"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136976"/>
    <dbReference type="eggNOG" id="KOG1614">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038194_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK42"/>
    <dbReference type="OMA" id="GPQFENG"/>
    <dbReference type="OrthoDB" id="10264038at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11368">
        <property type="entry name" value="RNase_PH_RRP45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033100">
        <property type="entry name" value="Rrp45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF14">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="7">
        <location>
            <begin position="34"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="8">
        <location>
            <begin position="190"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="304" mass="34815" checksum="B933CBC5C72E3D49" modified="2011-11-16" version="1">MSRSIEISENQRKFLLESLKHGFRLTDRPFDHVRDISLYLSPTEYGYVEVSWGKTKLACRVTATVTTPYEDRPFEGIFTINSEISTMASMRFDTSRSNEEILIARIIEKAVRRSNSLDLESLCIIAGEKVWEITVDLNYLNYDGNFIDVGCFAVMLALHHFKKPDISINGNSIVVHDINERQPVPLSILHIPICLTYSFYNLTDKESNIKGEDADEICLLDADLLEELNRDGSLVITLNKNRELIQLSKNGGLPISAQQFLELTFKSMKTIDQLTDLIKTKIKEHEELRYKTENFKLLEASADR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKC0</accession>
    <name>G3AKC0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60922</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375094.1">
        <property type="nucleotide sequence ID" value="XM_007375032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKC0"/>
    <dbReference type="FunCoup" id="G3AKC0">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="GeneID" id="18873635"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60922"/>
    <dbReference type="eggNOG" id="ENOG502RXX6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084558_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKC0"/>
    <dbReference type="OMA" id="TIGWAPL"/>
    <dbReference type="OrthoDB" id="2103474at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025187">
        <property type="entry name" value="DUF4112"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35519">
        <property type="entry name" value="MEMBRANE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35519:SF1">
        <property type="entry name" value="YALI0C06193P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13430">
        <property type="entry name" value="DUF4112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="232" mass="26304" checksum="10473DBF8A55A6FA" modified="2011-11-16" version="1">MSQIQQQLENIPGYDIVMDYFNDYAEEHFNSVDPYEDEDGNKRKLPADITNKREQKAWKKIQSQAWTHDKCFLGSCGVGMDCGLGLAPIVVFIFPVLGPIMLYLVHARLIRLAQENFNLPNKLVARLQSNIVIDLVITFPPLIGSFFGWLHGCSTRNAGLIYGFVSKMAEVRASAQGVQYVGTQRTIQQQPEFGSRGMTYQRQEPQNERRTKFTRNNPNAIVVGQQQQSGFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKD1</accession>
    <name>G3AKD1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Association with the SNF1 complex (ASC) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_60230</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 5'-AMP-activated protein kinase beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374403.1">
        <property type="nucleotide sequence ID" value="XM_007374341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKD1"/>
    <dbReference type="FunCoup" id="G3AKD1">
        <property type="interactions" value="447"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKD1"/>
    <dbReference type="GeneID" id="18873287"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60230"/>
    <dbReference type="eggNOG" id="KOG1616">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033562_1_0_1"/>
    <dbReference type="InParanoid" id="G3AKD1"/>
    <dbReference type="OMA" id="MEQYYVA"/>
    <dbReference type="OrthoDB" id="531008at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140767">
        <property type="term" value="F:enzyme-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000562">
        <property type="entry name" value="Snf1 kinase complex beta-subunit Gal83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006828">
        <property type="entry name" value="ASC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037256">
        <property type="entry name" value="ASC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050827">
        <property type="entry name" value="CRP1_MDG1_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343:SF84">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04739">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01010">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160219">
        <property type="entry name" value="AMPKBI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Association with the SNF1 complex (ASC)" evidence="5">
        <location>
            <begin position="281"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="211"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW32888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="407" mass="46581" checksum="9F4AD24BDCE87AED" modified="2011-11-16" version="1">MYSTASSETKDVSMVDVSKADEEGNGAPARRKSTLILTDDIDLDQPNADKQNNSNEDDRMNIDSSSANLTQHNQQQQQQQQQQQSQEQQNQQQQQQQTRQEDQAPPQPAQQPDPNVTVPVDIQWVQGGEKVYVTGSFTGWRKMIGLVRQPDNSFLITLGLPLGTHRFRFVVDNELRFSDFLPTATDQMGNFVNYIEITQENIEQHLAQLEENEAKQQQQQQQQQQQQQQQQQQLQQPQPEESRSRKSSLSRNRTDSMWGLINDDDDMGNGYSRYHDEDGTPLNKEYQYINDIPPIFTDPKVMEQYYVAIDKQSRSSNGQQQAWLHPPQLPPHLENVILNNFNNSSGENNSGALPIPNHVVLNHLATTSIKHNTLAVASIVRYKRKYVTQVLFSPLQQSLQPPEPRPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKJ7</accession>
    <name>G3AKJ7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60938</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375117.1">
        <property type="nucleotide sequence ID" value="XM_007375055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKJ7"/>
    <dbReference type="GeneID" id="18873645"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60938"/>
    <dbReference type="HOGENOM" id="CLU_2905610_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKJ7"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="28"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="62" mass="7321" checksum="C681D91D13243D05" modified="2011-11-16" version="1">MSFQRTEHKEHNHVDVNGTIFPIKNDENRSILSMPYECIFEVLQYLNQIDLVNVCLVNRVLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKK9</accession>
    <name>G3AKK9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_60947</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375129.1">
        <property type="nucleotide sequence ID" value="XM_007375067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKK9"/>
    <dbReference type="FunCoup" id="G3AKK9">
        <property type="interactions" value="229"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKK9"/>
    <dbReference type="GeneID" id="18873652"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60947"/>
    <dbReference type="eggNOG" id="KOG1291">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020088_4_1_1"/>
    <dbReference type="InParanoid" id="G3AKK9"/>
    <dbReference type="OMA" id="PWMQFYQ"/>
    <dbReference type="OrthoDB" id="409173at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015086">
        <property type="term" value="F:cadmium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01197">
        <property type="entry name" value="nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037982">
        <property type="entry name" value="Nramp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF29">
        <property type="entry name" value="IRON TRANSPORTER SMF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00447">
        <property type="entry name" value="NATRESASSCMP"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="295"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="339"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="386"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="416"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="483"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW33614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="506" mass="56059" checksum="A6D303008FED71A8" modified="2011-11-16" version="1">MLESIQLKRSKVLQVLRKYMSFIGPGLVVSVSYMDPGNYQTSVSSGSMFEYKLLIVIFMSNLFAIILQILCVKLGTVTGLDLAEMCRLHLNPKVNLFMYICAEIAIIATDLAEVVGTAIALEILFGIPLLYGVLITVLDVLIILMAYRKDGTMKQTRFFEIFVSILVAATCVCFVTELFKCDFPPGTGWQIFKGFLPVNDVVFTDSTALFLSCGIVGSTVMPHSLYLGSSLVQPRLKEYDENHDKEVVEVDDDERLFPPNTPRLRRLSDSSFLARKYKPSYAAIKYCLHYSYFELVLSLFTVAVFVNSAILIVAGSSLFGQPDAADADLLSIYKMLSYYISPAAGLVFALSMLFAGQSAGIVCTMAGQIISEGFINWNMKPWKRRIITRVLAIVPVVIVIGILGREGVSKVMNSSQVVLSFILPIVSGPLIYFTCSKEYMTVYETSSQATESTSLLENDVHSNSSSPCRYTFENGKFLRTASILTWVIISFLNIYLVIAFANGAQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKL9</accession>
    <name>G3AKL9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_66558</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375139.1">
        <property type="nucleotide sequence ID" value="XM_007375077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKL9"/>
    <dbReference type="GeneID" id="18875105"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66558"/>
    <dbReference type="eggNOG" id="ENOG502SCJR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_315504_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKL9"/>
    <dbReference type="OMA" id="IHQCHLT"/>
    <dbReference type="OrthoDB" id="4016549at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="652"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="305"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="588"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="774"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="862"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="311"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="327"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="355"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="395"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="410"/>
            <end position="431"/>
        </location>
    </feature>
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        <location>
            <begin position="446"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="478"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="588"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="788"/>
            <end position="824"/>
        </location>
    </feature>
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        <location>
            <begin position="831"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="862"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="915"/>
            <end position="926"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="926" mass="106748" checksum="F5267DEB4A981AD6" modified="2011-11-16" version="1">MTTQTKDLSDFCLSFPKPIDELHPDELVKKISQRIPRSLPACDNYYHAPAPTNSNTTPEKEDPIVKELSKLYPHKSGVVTYRVDHHEENEIVVYSGKLQELLYLLLEEKHLDDNIRISIESSLFDLTCYLPNPQVEEQPEEPEEIPKLTRKQKLRKQYLLKPNDAVSHLSYPNNYRSKIVIDNYEYLLKFSSTYFNLSLASHITKFMVELIYSLNYWEVVHLVYMNPDITYFLKLIDFEIIETSFGPLIKPPENYLCDDMLQGLQYPFPYPFYNYSYHSFDSRIEQNKFDSVKINPYLGISLKQQDMEPPKKKKKRGRKPKLRLETEDIEEPVDEEAVSPKHQDSPDYEEPQTFEVERDDDKDLSTTEEIQSVKEETTSIKQEPQQEYYDTPTLPGQVQQMPHPQQEQNVPPPPPPPPPPPPPQVYGPPPSLAMYAMSRPMQPHEQYSYYQYQQQDPQQGTSPRELPIPRVSPDNQGQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQHSLPSIQVQPEYQPVLINQRYEYRPGEVLPPINKLTEPRQIQDQQSQQSQQMPMFPFQNQLKFIPQSPNRAQSSGGPLAINTSSISPTIDSPQSGLLGSPRVDGDSSMHQMMDQSYMMDDKGKKKPGVIHQCHLTDPTTMSKCLKIFYGKNELLRHQEFVHATKKKIYKCIYCSRNGAKVQSYPRHDSLARHIRRKHGVTGKENKMAVNYAKENVEIIDESNQLVTQPHNFVEPLPHPGFLNQDYSVKDTGGFWSFRSKGQGEGGYEQQQQSSSPNQQQQQAISPEIQQGPQQGPIISLPPLGARDDGQYQGQPQQQSSMPMYMPVQATLIGVPEYRPNAQAQAQAQVSPQVEQQPTQQQQSQPNPTSGRTTTVLPSMHELDSSHSFGIHSRPFADSDQHKSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKR5</accession>
    <name>G3AKR5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">6-chlorohydroxyquinol-1,2-dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_136524</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the intradiol ring-cleavage dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374484.1">
        <property type="nucleotide sequence ID" value="XM_007374422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKR5"/>
    <dbReference type="GeneID" id="18869978"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136524"/>
    <dbReference type="eggNOG" id="ENOG502QWDJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046727_2_0_1"/>
    <dbReference type="InParanoid" id="G3AKR5"/>
    <dbReference type="OMA" id="RCVMRSD"/>
    <dbReference type="OrthoDB" id="5238185at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018576">
        <property type="term" value="F:catechol 1,2-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009712">
        <property type="term" value="P:catechol-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.130.10">
        <property type="entry name" value="Aromatic compound dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007535">
        <property type="entry name" value="Catechol_dOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000627">
        <property type="entry name" value="Intradiol_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015889">
        <property type="entry name" value="Intradiol_dOase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050770">
        <property type="entry name" value="Intradiol_RC_Dioxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33711">
        <property type="entry name" value="DIOXYGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G02910)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33711:SF7">
        <property type="entry name" value="INTRADIOL RING-CLEAVAGE DIOXYGENASES DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00775">
        <property type="entry name" value="Dioxygenase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04444">
        <property type="entry name" value="Dioxygenase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49482">
        <property type="entry name" value="Aromatic compound dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00083">
        <property type="entry name" value="INTRADIOL_DIOXYGENAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Intradiol ring-cleavage dioxygenases" evidence="7">
        <location>
            <begin position="137"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="326" mass="36108" checksum="CB34F43B00668703" modified="2011-11-16" version="1">MTPAATRLVDMSDETITDNAIAVNTNTVNNPRLQFVLSKLIQHLHDFARDTRLTTEEWMTGIQFLTECGDITTDIRSEFILLSDVLGLSVLVDSISHPKPPSATAGTLLGPFHTHDAEVKQQGESISSSGKGEPVVITGFLTDTDGNPIPDATIDLWHCDAHGKYDTQYSDRTHPDMRGIVRTRSDGSFLIRATKPVPYSVPDDGPVGKLLHNVGRHAMRPAHIHFIIKKPGYETLITALYGKGDPYQFSDAVFGVKSSLLFELERLGSRAKEFDMNEEDWILRWDFKIISEQEGQSLRVERNRTAIKGVEGVTLNEDGLPVAALD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALL2</accession>
    <name>G3ALL2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_137494</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374770.1">
        <property type="nucleotide sequence ID" value="XM_007374708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALL2"/>
    <dbReference type="FunCoup" id="G3ALL2">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALL2"/>
    <dbReference type="GeneID" id="18870047"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137494"/>
    <dbReference type="eggNOG" id="ENOG502RZVD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041801_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALL2"/>
    <dbReference type="OMA" id="LAQYWTD"/>
    <dbReference type="OrthoDB" id="5378975at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031355">
        <property type="entry name" value="YBL010C/LAA2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38698">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38698:SF1">
        <property type="entry name" value="FUNGAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17104">
        <property type="entry name" value="YBL010C_LAA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="313" mass="35679" checksum="E27DE1BEF37128D7" modified="2011-11-16" version="1">MSEEEQPQHAEIHEPATEVVVDVQEDNSGIDNDDDDDFDDFNEAGNDNDDDDFGDFDNTSYVQSTHPSFPEDVLNDSQQFIENLNKLMDSIFPSSDSEPRPTPNADENNNTSSPLLNERAATIFQQVSTLPYLHPTSWTKSNIRHSLLVQLGIPINLDELAQPPHASSTLSNLTRRKSITSASDIDWTVFDIPEFDKLDISSEQESRLLESTFDTLAMIEYDNLANCSSGYLEAASIEAVDEKLHQLESHYDELIKLSSCWQHRMAELHSDFEIYEGVVQSFIGYSQKLRRQEILEENLKKVKHTSTKKKFWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALT4</accession>
    <name>G3ALT4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_60051</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374208.1">
        <property type="nucleotide sequence ID" value="XM_007374146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALT4"/>
    <dbReference type="STRING" id="619300.G3ALT4"/>
    <dbReference type="GeneID" id="18873198"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60051"/>
    <dbReference type="eggNOG" id="KOG0427">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_15_1_1"/>
    <dbReference type="InParanoid" id="G3ALT4"/>
    <dbReference type="OMA" id="WQMDIKV"/>
    <dbReference type="OrthoDB" id="406833at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23808">
        <property type="entry name" value="UBCc_UBE2W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000072">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="4">
        <location>
            <begin position="5"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="171" mass="19989" checksum="4AF8A40E0342F079" modified="2011-11-16" version="1">MSRHPFHRRLLKEYQSLSQATLPGIKLVSNDENLTNFIFQIRIANHNLYPDYERYYLSINITQDYPVDCPQVKFIIYESVDDDKEDSDSIEIIEKSKIPIHPHIYSNGHICLNLLGEDWTPACSIESILLSIQSMLNTNDKLERPPDNDSYIKHAPLDPKRSRFVYHDDNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALX5</accession>
    <name>G3ALX5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_66302</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374843.1">
        <property type="nucleotide sequence ID" value="XM_007374781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALX5"/>
    <dbReference type="GeneID" id="18875097"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66302"/>
    <dbReference type="HOGENOM" id="CLU_2279244_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALX5"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="102" mass="10427" checksum="56F95BD16A6EBEA4" modified="2011-11-16" version="1">MAAMSGEHAGHDMGNMATSSTTDAVSSATSSTHDMAAMSHDHAGHNMESIATTSDHHHHTIESTVSSSAGNETATITSQFENGAGLISIGSTALFLLAWSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM88</accession>
    <name>G3AM88_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nucleolar protein 12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_50271</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in pre-25S rRNA processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRM RBM34 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374901.1">
        <property type="nucleotide sequence ID" value="XM_007374839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM88"/>
    <dbReference type="FunCoup" id="G3AM88">
        <property type="interactions" value="862"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM88"/>
    <dbReference type="GeneID" id="18871423"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50271"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006468_0_0_1"/>
    <dbReference type="InParanoid" id="G3AM88"/>
    <dbReference type="OMA" id="NAYAVYT"/>
    <dbReference type="OrthoDB" id="442677at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF25">
        <property type="entry name" value="RNA-BINDING PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="167"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="273"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="375"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="429"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="60"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="94"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="432"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="445"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="463" mass="52256" checksum="AE69A24CD381674A" modified="2011-11-16" version="1">MSSFASLFENSNVDKSVDDLFSSKADGPVSREQLAKGRTVVVVPKRKKEEKPDDDKKQEDQEDNDESEDDEEAESDDDEEAESEDEELEDSEEEQPKKKSKKSDANDDLEARYFDKLLQDESKPDQKEKEEESSSSEEADKAVSESKSSKPAKTVDLKETELEKAERTVFVGNVPASVITSKLIAKQFKNLFKAYGKVDSMRFRSISFEENLPRKISFAKKNLHKSRDSVNAYVVFKEKGASMDSRKLNGTVFEDHHLRVDHVAHPAAKDTKRSIFVGNLDFEEKEDNLWKYFNKKLDNDVESVRIIRDSKTNLGKGFALVQFKDSLSVNKALLLNDKPINVDGKNGRKLRISRAKSHAKPSLMSPNHIDNLKKKHASNKSKQLNDTQKTKLGRAKTILGKADRSTVGKSKKIIMEGERATKGTRVAGIKGLKGKAGKVKKPRIRDRSTKFKQEREALSKSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM96</accession>
    <name>G3AM96_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_55275</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374909.1">
        <property type="nucleotide sequence ID" value="XM_007374847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM96"/>
    <dbReference type="FunCoup" id="G3AM96">
        <property type="interactions" value="515"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM96"/>
    <dbReference type="GeneID" id="18871924"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55275"/>
    <dbReference type="eggNOG" id="ENOG502QRT9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008791_2_0_1"/>
    <dbReference type="InParanoid" id="G3AM96"/>
    <dbReference type="OMA" id="IQEQQIH"/>
    <dbReference type="OrthoDB" id="3366990at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010833">
        <property type="term" value="P:telomere maintenance via telomere lengthening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11660">
        <property type="entry name" value="SANT_TRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000137">
        <property type="entry name" value="MYB DNA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013867">
        <property type="entry name" value="Telomere_rpt-bd_fac_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052833">
        <property type="entry name" value="Telomeric_DNA-bd_trans-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47807">
        <property type="entry name" value="PROTEIN TBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47807:SF1">
        <property type="entry name" value="PROTEIN TBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08558">
        <property type="entry name" value="TRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="584"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="584"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="498"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="655"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="76"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="499"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="527"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="691" mass="77568" checksum="7695AE09587A36B0" modified="2011-11-16" version="1">MSDSHEISGNISEEINKEQEELVNQPSSTVQTGEEQNKTIQPSTEDGETQNEEVNAQSEQQHEDELNDIDTLFHTSVEDAKQEREEAGQEHNQHNKRNLEEESTKSEQQIQTDSSHPNEEQQQQQTQSEDIAEENEPVNEKNDLEMIANQLETATEANKEKEPETTTIDGITIPANSELLNTNTALAAYNALASELPPVAALASANLHGLPLPIVAPDYLPPRIQLLINTLPTLDNLATQLLRIVASGPYQKIIDLASNPDTPAGATYRDLTSLFEFTKRLYSEEDPFLTVEHIAPGMWKEGDMTPTMFRNREQSIESTLRKVNLATFLAATLGTIEVGFFYLNESFLDVFCPANNLELENALSNMGINNQSLQSGNFSIGEKVGKLLKPQATLYLDLKTQAYISAIEAGERSREEILEDILPNNLDEILLSRRGTKALSPMELDFVDRCNTRKEILLNYEGNLSEEYEWFTFLKELFDYVGKNVGFLIWGKRGRRERVHKEDTSATPHSDEILQRETPEAQPRVPVQNSITNAPSTEPTNDSTAIESSEDYQNLTSTLLPSEIVERQIHLRINPGTTSRTGHRRPWTRDEEKALRHALELKGPQWATILELFGNGGKISEALKNRTQVQLKDKARNWKMFFLKSGLPVPGYLSRVTGDLDRESKPRTKGPRSKKTAAAPVPSVQTSQKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMD8</accession>
    <name>G3AMD8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60138</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374305.1">
        <property type="nucleotide sequence ID" value="XM_007374243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMD8"/>
    <dbReference type="STRING" id="619300.G3AMD8"/>
    <dbReference type="GeneID" id="18873235"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60138"/>
    <dbReference type="eggNOG" id="ENOG502RQ0A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1075888_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMD8"/>
    <dbReference type="OrthoDB" id="4026708at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="259" mass="28200" checksum="7DF528C2CDBC29E7" modified="2011-11-16" version="1" fragment="single">MIDDSGKAVLSNDFFASFGKCDDSTIKKEEEPVKHEQAENVMTSTRLVHSNSVIGIESVYQQEEKSNHQQQQEEEEQEQGTPKMNTTSIPVPSIPLRRYNSDLTGITTTTILQNQVLSSISESSIIPQTPKDSYFNAANLGTGLTPIFNLTPQFNSLMYSMMNINSPKRGGSAGNGVNLITPDFFSSQQQEQRSQLQGLEEEARLETSTVTMNELMGQTVMKEKAVNDSVPSSSSSNGGGAQTSGWSSSEDSGDARLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMW6</accession>
    <name>G3AMW6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dihydroxyacetone kinase</fullName>
            <ecNumber evidence="5">2.7.1.28</ecNumber>
            <ecNumber evidence="4">2.7.1.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Glycerone kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Triokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Triose kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">SPAPADRAFT_139806</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-glyceraldehyde + ATP = D-glyceraldehyde 3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13941"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.28"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>dihydroxyacetone + ATP = dihydroxyacetone phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15773"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16016"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.29"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Polyol metabolism; glycerol fermentation; glycerone phosphate from glycerol (oxidative route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dihydroxyacetone kinase (DAK) family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.28" evidence="5"/>
    <dbReference type="EC" id="2.7.1.29" evidence="4"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375656.1">
        <property type="nucleotide sequence ID" value="XM_007375594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMW6"/>
    <dbReference type="FunCoup" id="G3AMW6">
        <property type="interactions" value="405"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMW6"/>
    <dbReference type="GeneID" id="18870239"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139806"/>
    <dbReference type="eggNOG" id="KOG2426">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017054_6_0_1"/>
    <dbReference type="InParanoid" id="G3AMW6"/>
    <dbReference type="OMA" id="FINDGTT"/>
    <dbReference type="OrthoDB" id="1724672at2759"/>
    <dbReference type="UniPathway" id="UPA00617">
        <property type="reaction ID" value="UER00669"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004371">
        <property type="term" value="F:glycerone kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050354">
        <property type="term" value="F:triokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019588">
        <property type="term" value="P:anaerobic glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061610">
        <property type="term" value="P:glycerol to glycerone phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.340:FF:000001">
        <property type="entry name" value="Dihydroxyacetone kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1180.20:FF:000001">
        <property type="entry name" value="Dihydroxyacetone kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10440:FF:000001">
        <property type="entry name" value="Dihydroxyacetone kinase, DhaK subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10440">
        <property type="entry name" value="Dihydroxyacetone kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1180.20">
        <property type="entry name" value="Dihydroxyacetone kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012734">
        <property type="entry name" value="DhaK_ATP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004006">
        <property type="entry name" value="DhaK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004007">
        <property type="entry name" value="DhaL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036117">
        <property type="entry name" value="DhaL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050861">
        <property type="entry name" value="Dihydroxyacetone_Kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02361">
        <property type="entry name" value="dak_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28629">
        <property type="entry name" value="TRIOKINASE/FMN CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28629:SF4">
        <property type="entry name" value="TRIOKINASE_FMN CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02733">
        <property type="entry name" value="Dak1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02734">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01120">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82549">
        <property type="entry name" value="DAK1/DegV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101473">
        <property type="entry name" value="DhaL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51481">
        <property type="entry name" value="DHAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51480">
        <property type="entry name" value="DHAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DhaK" evidence="20">
        <location>
            <begin position="11"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="DhaL" evidence="19">
        <location>
            <begin position="390"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-hemiaminal-histidine intermediate" evidence="17">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="57"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00075491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00079901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00083754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR612734-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR612734-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EGW32380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="594" mass="64444" checksum="AD33828C18584D8C" modified="2011-11-16" version="1">MTLSKHWKYNKDEDIVVTQLKGLVASNPAVQLIPSEKVVFNPHSVTSQKITLISGGGAGHEPLHGGYVGHNLLDAAVSGSIFASPSTKQIMAAVKTKSDKDKGTIIIVKNYTGDVLHFGLVAERAKSEGYKVEVVNVSEDVAVGREQNKMVGRRGLAGTSLVHKILGAAVNSEKGGDLTTVAQLGRVVNSNLVTLAASLDRTSVPGKTEEIEFNEADEAELGLGIHNEPGTKIKPIPQINELIKDMFHKLVSPEDKDRHYVDFDLKNDNYVLLINNIGGSSSFEMFAITQHVIENLPFSKKPKRVYVSDFVTSFNSPGFSITLLNLTNVSKEDIIFNSDDILKYLDAPTNAPGWKPKIFNDKLWEQPIEYVESPMKHQVAVTSKLRIDENKFETQLRNAMKVLLEQEPDITKYDTLVGDGDCGETLAKGANAILNALDNDKDFKSGLSDPVATVSRITELVEDSMGGTSGGLYSIYLTALAQNLQKSQEISVSALGDAFHNALYDGLFKYTRARVGGRTLVDTLQPFVDGLKKTGDLKEAVESAKKGCESTKHLHASFGRASYVNEQEFKAEGGVPDPGAVGLLAIIQGFLGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMY6</accession>
    <name>G3AMY6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">General transcription and DNA repair factor IIH subunit TFB5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_55858</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the 7-subunit TFIIH core complex composed of XPB/SSL2, XPD/RAD3, SSL1, TFB1, TFB2, TFB4 and TFB5, which is active in NER. The core complex associates with the 3-subunit CTD-kinase module TFIIK composed of CCL1, KIN28 and TFB3 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TFB5 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375676.1">
        <property type="nucleotide sequence ID" value="XM_007375614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMY6"/>
    <dbReference type="FunCoup" id="G3AMY6">
        <property type="interactions" value="157"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMY6"/>
    <dbReference type="GeneID" id="18871981"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55858"/>
    <dbReference type="eggNOG" id="KOG3451">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166246_3_2_1"/>
    <dbReference type="InParanoid" id="G3AMY6"/>
    <dbReference type="OMA" id="IYNPMDE"/>
    <dbReference type="OrthoDB" id="354at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006294">
        <property type="term" value="P:nucleotide-excision repair, preincision complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1220:FF:000002">
        <property type="entry name" value="RNA polymerase II transcription factor B subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1220">
        <property type="entry name" value="TFB5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035935">
        <property type="entry name" value="TFB5-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009400">
        <property type="entry name" value="TFIIH_TTDA/Tfb5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28580">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28580:SF1">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06331">
        <property type="entry name" value="Tfb5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01395">
        <property type="entry name" value="Tbf5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142897">
        <property type="entry name" value="TFB5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 10" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="69" mass="7768" checksum="47DD915351620BFF" modified="2011-11-16" version="1">MLVASKGVLVQCDPSIRALILQIDAESPGIVLEELDDTHLLIQHDKVQIVKNELNKLLSKNIYNPFDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN70</accession>
    <name>G3AN70_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_152113</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375188.1">
        <property type="nucleotide sequence ID" value="XM_007375126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN70"/>
    <dbReference type="STRING" id="619300.G3AN70"/>
    <dbReference type="GeneID" id="18870917"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_152113"/>
    <dbReference type="eggNOG" id="KOG1502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_9_2_1"/>
    <dbReference type="InParanoid" id="G3AN70"/>
    <dbReference type="OMA" id="RYICNAT"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05227">
        <property type="entry name" value="AR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="335" mass="37620" checksum="7CAAAB379570F432" modified="2011-11-16" version="1">MTTTTVFISGANGYLAQHIIKQLLVKGYKVIGSVRSHAKGEQLTNSVKDENFSYIVIPIITHKHAFYEVLLKHPEITVFLHTASPVTFEVEDNENDLLRPAIDGTISALKAIKRYAPQMERVVITSSAGAIFGFNEYFDSDTVYNEDSWSPLSYEQSKNTSAINGYFGSKKYAELAAWEFMKEQTPSFDITVVAPVYCFGPQAYGIKDKSKLNLSMQMVNTVLTLTEDDDIPEFGNIFADIRDVARAHIVAFESDEARGQRLLVATENFTFDKIANIISNHFVNVEIPPGNLSKNERIYKLMVPRYDNSKTNRILGFDFIGIERSILDTADQLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN89</accession>
    <name>G3AN89_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61541</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375748.1">
        <property type="nucleotide sequence ID" value="XM_007375686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN89"/>
    <dbReference type="GeneID" id="18873943"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61541"/>
    <dbReference type="eggNOG" id="ENOG502T62J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1994030_0_0_1"/>
    <dbReference type="InParanoid" id="G3AN89"/>
    <dbReference type="OMA" id="TIHIFHY"/>
    <dbReference type="OrthoDB" id="4093584at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="125" mass="14894" checksum="2022A759EEE37792" modified="2011-11-16" version="1">MSEQTTPVPRTRRVKQQSPYEVYIKPYVTPKLKKDLSFGLVGFLGMCVGIFHYAYIMKEWLMNPYMENTKLAIHFAGFFLHVFVSIYFYLFKYYPVVYAEEIAEEQAELEELRKKDAEIKSRKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANB4</accession>
    <name>G3ANB4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_51048</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with the U3 snoRNA and is associated with the 90S and 40S pre-ribosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DDX52/ROK1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375773.1">
        <property type="nucleotide sequence ID" value="XM_007375711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANB4"/>
    <dbReference type="FunCoup" id="G3ANB4">
        <property type="interactions" value="1059"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANB4"/>
    <dbReference type="GeneID" id="18871508"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51048"/>
    <dbReference type="eggNOG" id="KOG0344">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_4_1"/>
    <dbReference type="InParanoid" id="G3ANB4"/>
    <dbReference type="OMA" id="EMAHSIM"/>
    <dbReference type="OrthoDB" id="360161at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17957">
        <property type="entry name" value="DEADc_DDX52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000759">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044764">
        <property type="entry name" value="DDX52/Rok1_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF15">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13 17" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="145"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="334"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="522"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="31"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW32497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="546" mass="61983" checksum="7795035E5011B44A" modified="2011-11-16" version="1">MDIFRILTRGASLNKKKNVTTDHTLPKNTKNSHEKHKEETLHNQVERETDFFHTRKHSKPEEENKQETTEEKEDEDETPLLEINSEEDAQKFRKSHKSKVTGDDIPLPIGSFQDMIGRFNIDKKLLTNLLAAEFVEPTAIQCESIAISLSNRDLIACAPTGSGKTLAFLIPLIQKIITEPVGKNHGIRGLIISPTNELAHQIFNQLEAITKGYSKRLNCAILSKQLANKLNNKIINSSKYDIIVTTPLRLIDIVHNQAIDLSKVSNLIIDEADKLFDHGFAEQTDEILTHCTFPHIQKSMFSATIPSGVEEMAHSIMKDPIRVIIGHKEAANVTIDQKLVFTGNEQGKLLAIRQMIQQGQFKPPIIIFLQSITRAKALFHELLYDRLNVDVIHAERTPKQREEVINRFKSGDIWVLITTDVLARGVDFKGVNMVINYDVPQTAQAYVHRIGRTGRGGKTGTAVTFFTKEDDQLVKPIINVMKQSGCESGYSEWMEDMGKLSKKDKQKLKTHEVTRKQISTVPKVMKQKRKQRQQMIEASKKEKARY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANC4</accession>
    <name>G3ANC4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">VPS10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_138012</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375783.1">
        <property type="nucleotide sequence ID" value="XM_007375721.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3ANC4">
        <property type="interactions" value="208"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANC4"/>
    <dbReference type="GeneID" id="18870090"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138012"/>
    <dbReference type="eggNOG" id="KOG3511">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000700_0_1_1"/>
    <dbReference type="InParanoid" id="G3ANC4"/>
    <dbReference type="OMA" id="ECDYNYY"/>
    <dbReference type="OrthoDB" id="443634at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15482">
        <property type="entry name" value="Sialidase_non-viral"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.270:FF:000005">
        <property type="entry name" value="Sortilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.80">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031777">
        <property type="entry name" value="Sortilin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031778">
        <property type="entry name" value="Sortilin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006581">
        <property type="entry name" value="VPS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050310">
        <property type="entry name" value="VPS10-sortilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106">
        <property type="entry name" value="SORTILIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106:SF27">
        <property type="entry name" value="SORTILIN-RELATED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15902">
        <property type="entry name" value="Sortilin-Vps10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15901">
        <property type="entry name" value="Sortilin_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00602">
        <property type="entry name" value="VPS10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110296">
        <property type="entry name" value="Oligoxyloglucan reducing end-specific cellobiohydrolase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442494" description="VPS10 domain-containing protein" evidence="9">
        <location>
            <begin position="20"/>
            <end position="1551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1374"/>
            <end position="1393"/>
        </location>
    </feature>
    <feature type="domain" description="VPS10" evidence="10">
        <location>
            <begin position="45"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="VPS10" evidence="10">
        <location>
            <begin position="727"/>
            <end position="1359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1520"/>
            <end position="1551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1520"/>
            <end position="1537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1538"/>
            <end position="1551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1551" mass="174840" checksum="20695D742D8B1A27" modified="2011-11-16" version="1">MLLSNLVIFLSLLIASVRGDTSYTPDITTVTSDHVAREIRYFDDSSNVLLLRQTTLSISFDDGKSFEKVKTISEPVVYFQMDPFNRNRAFAFTLSENQYVTEDKGRNWRKFKADLHIDENSNSFPQISFNVANPDYLMISSYKCPPQEPFTFRCKQTYYYTKDGFKSVKKLPLDGAHICKFVKSTKTFKADKDETILCITNDINSFGHVVASHLYKSDNFFSDKQEITLGKQSDSGIIIDIKVEETFIIAIVNLDKFNEKSRVHLYISKDGQTFAKADLEIDVKYGVMSFLPSSASSLFFQILQYSNDKFQASKLYSSDSFGLHYTTDLEQIAGGNIQKVENIDGAWIANVGVDKSDNNDDESSGKSVLDYLVGGIFSKNIITKYSFNDGREWNLLKSTNEDCKISSGCSVHLWEYSELDGEGKFVTGPTPGILMGVGNTGSHLNKDFSKLRTFVSRDGGASWTQVLDFPTIFSFGDQGNVIVAIPFGGKGRMKPSDKVYYSLDQGKSWNNVDLKNPIYPLAVLTTIDGTSRNFLLAGATSERTEVIYTLDFTKAYDGVTCKDDAYEEYYARSIADNPPLCIYGHREKFRRRKQDQACFVNKLFEDVKVIDEPCPCIDLDFECALGFKSSPKDQHICIPDPAQLANMCKGQKELKLEDKVLIDGDKCDLGKKKLSDFIVKETIDCTKYNNDKEKPGNTHDIVVNVNEIEGELVQYAYIDESKQFGADNIVLKSSDNHVYVSNSGGVSFVRVPIPDKIVAFYTGPIPGQVILITESKLIYVSRDGGATFIKQQAPTPPHPLVPRAVAFHTSNPDKFIWFGGDCESQSDCPVTAYITDNSGSDFFKVPLKNVQTCDFVGAVFENPVEDLLFCASRDGDNKLKLVSLTAGSNEEPKVLYPHIVGYAITGNYVVVATVNQKDDKRTTLEAKVTVDGKVFADADFPHDFQLDSQQAYTILDSKTKAIFLHMTTSLEPNFEYGAILKSNSNGTSYALVLDAVNRNREGYVDYDRIDDGLEGVIISNIVANPGAKNSKKQIQTMITHNDGGEWNFLLPPAVDSKNQKYKCQGSKCHLHLHGFTERADYRDTYSSGSATGFLIGAGNVGEYLEDYDKSSTFMSKDGGVTWKEIKQGNYMWEYGDHGTILVLVSNYEETNTLLYSLDEGESWIEYKFADSKVRVLDLATVPTDTSRKFVIFAKDGDQRKTLAYSIDFTNIHPRQCQLDLDHPDKDDYEYWSPMNPSSLDVEEPKCLFGHEAKYLRRAKGHYDCFIGSAPVKDGFKVIRNCSCTRRDYECDYNYYRDTDNTCKLVKGLTPEDKKKEMCEKPGAFEYFVSTGYRKIPLTTCEGGKQFDSWNPLPCPGKQKEFNHHYGTEVTGHKLFLLLFVPLVIFLGAFWFVYDRGIRRNGGFKRLGQIRLDDDENGFDPIENNQVDVIVNRVVKGGIFAVAITVATFKTIIKIDKMIFERMTSVFLGRAPGRRNYVNVPDEFEDEEAALFGDYDDDEEELQDDTRLSEQTFRDDDIVDIEQHDQQEQVPADERLFDIESEDDLPIVTPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANM4</accession>
    <name>G3ANM4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_138844</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375829.1">
        <property type="nucleotide sequence ID" value="XM_007375767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANM4"/>
    <dbReference type="STRING" id="619300.G3ANM4"/>
    <dbReference type="GeneID" id="18870155"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138844"/>
    <dbReference type="eggNOG" id="ENOG502QPW5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_469435_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANM4"/>
    <dbReference type="OrthoDB" id="4025267at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051615">
        <property type="entry name" value="Transcr_Regulatory_Elem"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31313">
        <property type="entry name" value="TY1 ENHANCER ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31313:SF81">
        <property type="entry name" value="TY1 ENHANCER ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="8">
        <location>
            <begin position="174"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="581" mass="66133" checksum="AFCCCD46544E50BB" modified="2011-11-16" version="1">MLVNSSSRPSIPKLDSVKQEGDNNDGYTNGNENSETLIGEVKVDLQVLTSQKVSDLINIALTYFDHQILCYILNTRKTQELFEDLRAHVDEIGYAEQIFCTYPNNTYISPELIYSLSVIGSIYRDDLPGALFYYELSQRIIFHNYTLSKSSYPKLVALVLNAFFELNRGELSSAWQLSGMSFRMGFDLGLHDSTKEEPDEAMNNLKNLTYWGTFIIDNYAGYIFGRPKMLMYDCDYPIFNNGLNDIKLPHIVGLLAMGDPMITSIYQIMPFKDAEEFKRTFIVKYNQLREYNNRLIQWRNALPKELVWNMSSLKRSARITDHSFKFAFYLMFIILNKPFLLLPIGSDISMFTVICQEMEIIFRNVEDPCYLKNIVVLYVMILVCKLMLIQLLNRNSKKIDEQFERLEFFISYIKVVFKADVWILAKYPLAMLESRVMVLKKELKQHEQVNSQSTPAITSTPSDANAVQVKITGTSISSSSSSGRSNSIVDPMIPYTDENMSQNVGFLKMVDALFSQGTITPTQGMFPVSTAGTPTAAQEQIDNSLHISNNGATGATVQGSSETFNNIFSFDLLDLDAYMQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP58</accession>
    <name>G3AP58_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP-activated protein kinase glycogen-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_61690</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375905.1">
        <property type="nucleotide sequence ID" value="XM_007375843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP58"/>
    <dbReference type="STRING" id="619300.G3AP58"/>
    <dbReference type="GeneID" id="18874006"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61690"/>
    <dbReference type="eggNOG" id="KOG1616">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032652_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP58"/>
    <dbReference type="OMA" id="TFDNWTK"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050827">
        <property type="entry name" value="CRP1_MDG1_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343:SF84">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="3">
        <location>
            <begin position="2"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="150"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="351"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="438"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="362"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="489" mass="51431" checksum="2B054543F7CE4F83" modified="2011-11-16" version="1">MSYTFKWPSGPQDVVVTGSFVNWTENIPLVKQADGSFSLEVPFASSTEPILYKYVVDGVWQASQDEKITKDDSGIENNILDVDDLKALSTKAKSIIPESGLSVTQPELSTTVLPKEELKQVSVAGEPGIQIPTQPEALAAFNEVRDVDPATLNKEESPATSELTAEERKKLKKKVKRTQYKAKKKKKAAEGGAEASTEEDTEKEQTPEPLAAPVEAAVPAGINQVTQEAKAVVSEPASKFEEAKNAVVSESSTTVKAVEPEGVKEAVVSETSAAVQAAEPEGVKDAVISETSAVKAAEPEGIKEAVVSETSVAVKAAEPEGVKEAVVSETSAAVKAAEPEGVKEAVVSETSGAVASAGDQIKTLDPKATEPAEGKEVDASPVVKDEAPKTVEDEEEIIIAEGDKDAIAAAIAAGHTLEEVEPTEEQKAKLAGVVNGAKDAEGAKETAETAAKKAESTAKKAVNEASKKEVAKEEKKKGGFRRFLKKVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP59</accession>
    <name>G3AP59_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_61691</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375906.1">
        <property type="nucleotide sequence ID" value="XM_007375844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP59"/>
    <dbReference type="STRING" id="619300.G3AP59"/>
    <dbReference type="GeneID" id="18874007"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61691"/>
    <dbReference type="eggNOG" id="KOG0106">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054994_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP59"/>
    <dbReference type="OMA" id="EGVAWQE"/>
    <dbReference type="OrthoDB" id="1099063at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031370">
        <property type="term" value="F:eukaryotic initiation factor 4G binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061752">
        <property type="term" value="F:telomeric repeat-containing RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000805">
        <property type="term" value="P:negative regulation of termination of RNA polymerase II transcription, poly(A)-coupled"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006415">
        <property type="term" value="P:translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF62">
        <property type="entry name" value="SERINE_ARGININE (SR)-TYPE SHUTTLING MRNA BINDING PROTEIN NPL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="16"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="170"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="242"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="185"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="264"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="291" mass="32504" checksum="31EF2D5A6A8C2B44" modified="2011-11-16" version="1">MDEQAPAPAPAVEVSTQLFVRPIGFDVPREQLEEHFAPAGHVVDVQLMRGFAFVRFDNEEDATRAVETLNDSEFDGQRLLVEYAKERKEDTRGKYRVKLTNLEDGTAWQDIKDFVRDKTDSEPSYVKVFRNYDTNEIVGALEFSSAEELEKAIPLLDKAEFRGVVIGAEEDTSPFIPPPPRRGGFRGGRGGFRGGRGGYDRGFDRGGFRGGRGGYDRGFDRGGFRGGRGGYDRGFDRGGFRGGRGGYDGGFRGGRGGFRGGRGGYDRGDDRGDYRREERDSYVRERSPTRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3API2</accession>
    <name>G3API2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein uS7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_61235</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375429.1">
        <property type="nucleotide sequence ID" value="XM_007375367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3API2"/>
    <dbReference type="FunCoup" id="G3API2">
        <property type="interactions" value="1057"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3API2"/>
    <dbReference type="GeneID" id="18873791"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61235"/>
    <dbReference type="eggNOG" id="KOG3291">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063975_0_0_1"/>
    <dbReference type="InParanoid" id="G3API2"/>
    <dbReference type="OMA" id="KMNIVER"/>
    <dbReference type="OrthoDB" id="10264639at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14867">
        <property type="entry name" value="uS7_Eukaryote"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.455.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_uS7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005716">
        <property type="entry name" value="Ribosomal_uS7_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003106">
        <property type="entry name" value="PRK04027.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01028">
        <property type="entry name" value="uS7_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS7" evidence="5">
        <location>
            <begin position="68"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW32153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="225" mass="25233" checksum="B816057D43897B36" modified="2011-11-16" version="1">MSDVEEQYEEQPQEVVQEELQLVELATPIPTEVQEAQREVKLFNKWSFDDVEVNDVSLVDYIQIRSPVFVSHTAGKYASKRFRKAQCPIVERLTNSLMMNGRNNGKKLKAVRIVKHALEIINVLTEQNPIQVLVDAIVNSGAREDSTRIGSSGTVRRQAVDVSPLRRVNQAIALLTIGAREASFRNIKTIAECLAEELINAAKGSSTSYAIKKKDELERVAKSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3APM8</accession>
    <name>G3APM8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Post-GPI attachment to proteins factor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_61282</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the lipid remodeling steps of GPI-anchor maturation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PGAP3 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375475.1">
        <property type="nucleotide sequence ID" value="XM_007375413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APM8"/>
    <dbReference type="FunCoup" id="G3APM8">
        <property type="interactions" value="186"/>
    </dbReference>
    <dbReference type="GeneID" id="18873816"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61282"/>
    <dbReference type="eggNOG" id="KOG2970">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032917_1_1_1"/>
    <dbReference type="InParanoid" id="G3APM8"/>
    <dbReference type="OMA" id="DFMIEDC"/>
    <dbReference type="OrthoDB" id="419770at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007217">
        <property type="entry name" value="Per1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13148">
        <property type="entry name" value="PER1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13148:SF0">
        <property type="entry name" value="POST-GPI ATTACHMENT TO PROTEINS FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04080">
        <property type="entry name" value="Per1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2 7" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5016480365" description="Post-GPI attachment to proteins factor 3" evidence="7">
        <location>
            <begin position="19"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="371" mass="43944" checksum="F8A9F6B400FB843E" modified="2011-11-16" version="1">MRLLRLSFLAVLIRLAVGSPGDDLDDFQHCIEQCRQSTCQEGHVHYYNRAWSFVEMPLPLHLQLLGWTCDSNCDYQCQRIITQERKDRNEEVYQFHGKWPFLRVFGIQELFSVLMSLGNLFVAYLGFRKLWSCVTNTKLGSLRFQFVNALVLNVVTMFAWIFSTVFHIRDFLVTEHLDYYFAGLTVLTGFHAVGARVFSLYRPDRVLLRWSFTIGCISAYMYHIHRLITDWSYTYNMRANIFIGVWQNVFFALLCYTLYSKYYWLEQSEEKNLCHLNYIHFKQVILPSFYSSSPKLYSLYPLLLCTIVALGMSLEIFDFPPFFYDLIDAHSLWHLVTIIPAYMGWYDWIIWDISENVIGELVELETKKKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3APN8</accession>
    <name>G3APN8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_50783</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375485.1">
        <property type="nucleotide sequence ID" value="XM_007375423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APN8"/>
    <dbReference type="GeneID" id="18871483"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50783"/>
    <dbReference type="eggNOG" id="ENOG502SF6T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029128_0_0_1"/>
    <dbReference type="InParanoid" id="G3APN8"/>
    <dbReference type="OrthoDB" id="4073735at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF22">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN COMPLEX ACID LABILE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="492" mass="57176" checksum="F797CE6E1F3BAE3D" modified="2011-11-16" version="1">MIISYGDWSSSFLEHYFPRLPEDIQNCIYELSTGNDLLNLYTRFRNAAVHSSLSSYYSQEILFIPWGSSSSFSRRMDNNKRMLPIIRNNDDVIQFINEFPKVIPNRLHIHCNNYGFSSMQDILDMYGDRIYQIKELEITLAGYILLTPNKFNYLLSFPNLTTLQLYSTRWEKRLVKNSIPINHPTLRKLEFSQFVYGVGNDNVDYYIDWSGFEFPQNLQELSLTGIANISTITIPETTTHLHFNCAKIGDIVSCKSMLENVTELSLRWCRLQVIDLDVLPAGLRILDLSYNRIGRIVGNYKSALPEDLHTLKLNMCTLDDVVLDHINENIGWPSQLTRLEISSNNFSKIDILEKLPETLKTLWLSRNHLKWDEGEVLFTFPHLTELCLHRCGICDLQNFQFPSSLKNLTLCFNEIENISSYQGWNNLVNLHTLSLCRFSRQNLNGWIVLPNLRELHLAYTENDQLNNKFGVSSENYNLQIITSGCRYCNRNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3APQ3</accession>
    <name>G3APQ3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_50795</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375501.1">
        <property type="nucleotide sequence ID" value="XM_007375439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APQ3"/>
    <dbReference type="GeneID" id="18871485"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50795"/>
    <dbReference type="HOGENOM" id="CLU_1116339_0_0_1"/>
    <dbReference type="InParanoid" id="G3APQ3"/>
    <dbReference type="OrthoDB" id="10391567at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="249" mass="28303" checksum="210DB1B52DF6E95E" modified="2011-11-16" version="1">MSAQARLSMVLSHLQHRTKHELRPEFPLLPTEERAKYNGEEYYKIVPNISKVVSSVTPGTFRLNSPSGGFDELFNFISNRISEECPQGNIAFCDVIGVLEGFECDSNDRILYVSEDNSKLVEEYNVTYSNLKELDNETITGSYEFVILNHVKSSIMSDLTKFIRSTNPNAIIITVDNMKNLSKFEEWNSDVFIADSNVKLSVLRKDIVDKVKNEAPLNMSQAYKTLNLPTFTELSLLKLELCTRELIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3APU3</accession>
    <name>G3APU3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Autophagy-related protein 101</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_138327</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATG101 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375540.1">
        <property type="nucleotide sequence ID" value="XM_007375478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APU3"/>
    <dbReference type="STRING" id="619300.G3APU3"/>
    <dbReference type="GeneID" id="18870113"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138327"/>
    <dbReference type="eggNOG" id="KOG4493">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069661_1_1_1"/>
    <dbReference type="InParanoid" id="G3APU3"/>
    <dbReference type="OMA" id="VCWEIWT"/>
    <dbReference type="OrthoDB" id="10259639at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012445">
        <property type="entry name" value="ATG101"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13292">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13292:SF0">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07855">
        <property type="entry name" value="ATG101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0072">Autophagy</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="130"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="234" mass="26624" checksum="C4EFF939323FC435" modified="2011-11-16" version="1">MEFVLDLIAERSVIRESLKGVWIMWTVFFNRLFGPITPATNEFLNITYPIAVNLPDLDSLIDERINQLVKSNFDRRLPSKGVVNIQFLDKDGHIKKKTGWFGGKVDTQTSDNLKAWESWIINVECLPIDERPTETSSPQTDQPPATHPRDNNYSTNMVKSIKSFELNLIKIIDSVDRHKQHIPPITTLESSPFPYKISVLEAETVTATGSEASSSSVRTEQIDESWGDYIKKML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQA6</accession>
    <name>G3AQA6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase S54 rhomboid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_62024</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376231.1">
        <property type="nucleotide sequence ID" value="XM_007376169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQA6"/>
    <dbReference type="FunCoup" id="G3AQA6">
        <property type="interactions" value="631"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQA6"/>
    <dbReference type="GeneID" id="18874153"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62024"/>
    <dbReference type="eggNOG" id="KOG2980">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034022_2_1_1"/>
    <dbReference type="InParanoid" id="G3AQA6"/>
    <dbReference type="OMA" id="LWYPRIA"/>
    <dbReference type="OrthoDB" id="10260614at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010821">
        <property type="term" value="P:regulation of mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000012">
        <property type="entry name" value="Rhomboid family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050925">
        <property type="entry name" value="Rhomboid_protease_S54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731:SF14">
        <property type="entry name" value="PRESENILIN-ASSOCIATED RHOMBOID-LIKE PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731">
        <property type="entry name" value="RHOMBOID PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="262"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="8">
        <location>
            <begin position="167"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="328" mass="37262" checksum="39140D91EE7FAD20" modified="2011-11-16" version="1">MLSPFTRQFSTGLRTNLFFSNANLSSARYAKGLQKTITPRVPILKSAFGPNFNRQFTYFKKTIISQVNGNGIRRSTTWNNYNKYHNRSNWDRLKKPFIFTVAFSVATTVVTPYLFDYTPMKYFKRNPRALVWSIVALNAVVFLMWKVPSLQRYTMQYGIMFKDNLQSVWTMLGSAFSHQSFGHILVNMFALQSFGTTLCAVLGVANFTILYLNSAVISSFAALAIPTLMRSSLSFASLGASGAVFSVFAAFSYLIPKAPIALFFFPIPGGAWMLFLGTFAWNVAGVVLRWGVYDYAAHIGGSLVGLAYAYWYNIKRKEELRRRQIRWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQF8</accession>
    <name>G3AQF8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">AP-3 complex subunit delta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_67558</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Adaptor protein complex 3 (AP-3) is a heterotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376282.1">
        <property type="nucleotide sequence ID" value="XM_007376220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQF8"/>
    <dbReference type="FunCoup" id="G3AQF8">
        <property type="interactions" value="621"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQF8"/>
    <dbReference type="GeneID" id="18875170"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67558"/>
    <dbReference type="eggNOG" id="KOG1059">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001908_1_1_1"/>
    <dbReference type="InParanoid" id="G3AQF8"/>
    <dbReference type="OMA" id="SGNNWMA"/>
    <dbReference type="OrthoDB" id="10264595at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030123">
        <property type="term" value="C:AP-3 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030665">
        <property type="term" value="C:clathrin-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000251">
        <property type="entry name" value="AP-3 complex subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017105">
        <property type="entry name" value="AP3_complex_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22781:SF12">
        <property type="entry name" value="AP-3 COMPLEX SUBUNIT DELTA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22781">
        <property type="entry name" value="DELTA ADAPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037092">
        <property type="entry name" value="AP3_complex_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="10">
        <location>
            <begin position="51"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="682"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="852"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1004"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="682"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="852"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="890"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="900"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="945"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1025"/>
            <end position="1039"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR037092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW31504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1039" mass="118188" checksum="3488C88F4EC4EDC7" modified="2011-11-16" version="1">MSFQLQNSQVLARLKPFGISFEKSLSDLIKGIRSHSKESPEALAQFLDVAIQECKTELSTTDLETKAMAILKLAYLEMYGFDMSWCNFHILEIMSSGKFQQKRIGYLAAIQSFKNEQDLLILATNQFKKDLNSHNHIEIGLALSGIATIVTPNLAKDINDDVLMKLNHSKPYIRKKAILAMYKIFLQYPDSLRLNFQRVIDKLDDPDISVISATVNVICEISKKNPNIFINYLPKLFTILEDTKNNWLIIRILKLFQSLSKVEPRMKRKILPTIMDLMLRTQASSLIYECINCIVNGQMLSTESFKDSETAKTCIDQLMTFFKTNDSNLKFVGLLALINILKIFPIFIDRVEGVSQVIMDCLVDPDIIIKQKALQVCHYLVNEDNITEVVKLLLTQLIPTENSTVPQQLKLEVTLKLLEIATLDNYANIPNFKWYVAVLKDVINLTLLPLPSASTTISPQVSNSIAIELGKEFKNLATKVPSIRPTLLNNVITFIQDVRIIENCPSLLTDFYWIMGEYIDELREDSEDSDDEDDSQVILNKKIKLFNLLVNNNIDKSLGLTVHLNFPISAKLVRLGYPSVLAVVIQALVKLFNSIVSDYAKFYSSELPDDKYNEICYYLYKLVGFLSNWETHQNYEVQERALSWLEFLKLSLEAMTGDDLSAIKLSEEKDITFYKLLREAQVEESEEDSEEESEEEDSDDNSDDDSDEYGDLESDDEMVSTPIAPLPEFEPNPFAEIEQTEQLEQPVKTQEEHSQLPAEISLSEARKLPILLTTILPSFFKSYQLNPVSRNAQRSIAVPEDLDLDSEISSVPFTLELDPVQDNDSYNLYISEVEEEPLIQLTSEETFEKKKERMNRLKDDPYYINPEPIKTKKKSKSKTESESVTPESYTEMSTAPSVTKKSKKSKLKKEKVVILSEETVEGASPLTDLESSKKPKKKKNALKIDSSNLDNFNLSSSDVLDVSNKNEYEIDLESYRNKLAEAAQEEKPKKKKKSKTKKIIETEAEVESVHSTRSGTGSPDVITVKPKKTKKKTKAVILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQL8</accession>
    <name>G3AQL8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">VPS37 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_62174</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS37 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376343.1">
        <property type="nucleotide sequence ID" value="XM_007376281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQL8"/>
    <dbReference type="STRING" id="619300.G3AQL8"/>
    <dbReference type="GeneID" id="18874205"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62174"/>
    <dbReference type="eggNOG" id="ENOG502SBKC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1518435_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQL8"/>
    <dbReference type="OMA" id="LYRWEEE"/>
    <dbReference type="OrthoDB" id="10260857at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072666">
        <property type="term" value="P:establishment of protein localization to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043162">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037202">
        <property type="entry name" value="ESCRT_assembly_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009851">
        <property type="entry name" value="Mod_r"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07200">
        <property type="entry name" value="Mod_r"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140111">
        <property type="entry name" value="Endosomal sorting complex assembly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51314">
        <property type="entry name" value="VPS37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="5 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VPS37 C-terminal" evidence="7">
        <location>
            <begin position="103"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="183" mass="21787" checksum="55DB6F6F0478C0F1" modified="2011-11-16" version="1">MEKLHHQPPLNPLQDSELNAFPLPKNLELLPNSNIKEFVENKELIQGYIKQLEAYKHHQSKLLKKLSTVDELLSKDITQLIEQYQQLIVKITQQIQSISTIYQEFVNLETIQYQLLSSNFNSENLKHKFAKLTEDESKFDFGDSKENGEFSDFIERFRANRRVYHYRKEKLNRWNEERVGGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQS7</accession>
    <name>G3AQS7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cleavage/polyadenylation specificity factor A subunit C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62234</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376402.1">
        <property type="nucleotide sequence ID" value="XM_007376340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQS7"/>
    <dbReference type="GeneID" id="18874226"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62234"/>
    <dbReference type="HOGENOM" id="CLU_1644762_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQS7"/>
    <dbReference type="OrthoDB" id="433457at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="18384" checksum="04F84A1F43E6386F" modified="2011-11-16" version="1">MDIVAFNKLRYLVSGDSLGNIAVVNLLNEVIVCQFNLNDQINMIKSVKEEHPKLAIIATVSGGLYLLSESDSKIFESSGKKIMKNLEQFGTSVTHWKVLDKDIEELDDEFAPFDERVSTEFLGKLSCMENNQKVETFTSTYKQSYRHKQLLQDIHHQCHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQW3</accession>
    <name>G3AQW3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">allantoinase</fullName>
            <ecNumber evidence="6">3.5.2.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_72051</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-allantoin + H2O = allantoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17029"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15678"/>
            <dbReference type="ChEBI" id="CHEBI:17536"/>
            <dbReference type="EC" id="3.5.2.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nitrogen metabolism; (S)-allantoin degradation; allantoate from (S)-allantoin: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-dependent hydrolases superfamily. Allantoinase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.5" evidence="6"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375970.1">
        <property type="nucleotide sequence ID" value="XM_007375908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQW3"/>
    <dbReference type="FunCoup" id="G3AQW3">
        <property type="interactions" value="1267"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQW3"/>
    <dbReference type="GeneID" id="18875411"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72051"/>
    <dbReference type="eggNOG" id="KOG2584">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015572_4_0_1"/>
    <dbReference type="InParanoid" id="G3AQW3"/>
    <dbReference type="OMA" id="SRLHVCH"/>
    <dbReference type="OrthoDB" id="10258955at2759"/>
    <dbReference type="UniPathway" id="UPA00395">
        <property type="reaction ID" value="UER00653"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004038">
        <property type="term" value="F:allantoinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009442">
        <property type="term" value="P:allantoin assimilation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006145">
        <property type="term" value="P:purine nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000032">
        <property type="entry name" value="Allantoinase Dal1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017593">
        <property type="entry name" value="Allantoinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050138">
        <property type="entry name" value="DHOase/Allantoinase_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03178">
        <property type="entry name" value="allantoinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43668">
        <property type="entry name" value="ALLANTOINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43668:SF2">
        <property type="entry name" value="ALLANTOINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01137">
        <property type="entry name" value="TATD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="10">
        <location>
            <begin position="60"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW31192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="464" mass="50818" checksum="2242E41305EA7DBC" modified="2011-11-16" version="1">MSTALSSTRVLIGTKLVESTVIFSPDTGKIVAIISGIRGSSDPTLALYNVTKYKNVTPHVIMPGLVDTHVHLNDPGRTHWEGFETGTKSAASGGVTTIIDMPLNSIPPVTTVSNFQTKIDAAKDSAWVDLGFWGGLVPDNVNDLKPLINMGVRGFKGFLIDSGVEEFPAISKQHILQAMKEVEQEKTMLMFHAEMDSGATVDVDYSLDPTLYSTFLESRPDEFETQAIGEIITASIEFPTVPVHIVHVSSHQAIPLLAVAQKKQLPITAETCFHYLSLAAETVPSKSTHFKCCPPIRTENNRKLLWDGLRTGVITTVVSDHSPCTPQLKKLETGDFFEAWGGISSVGLGLPILFTEGQKMTPPVTLSEINQWCSINTAKQVGLSYTKGKLKVGYDADLLVFDTDAEYTIDNKDVHFKNKLTAYDGMKLQGRVVETFVRGKSVYSFEQGHSKVPLGRLILEPRTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQX0</accession>
    <name>G3AQX0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_61780</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375977.1">
        <property type="nucleotide sequence ID" value="XM_007375915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQX0"/>
    <dbReference type="GeneID" id="18874044"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61780"/>
    <dbReference type="eggNOG" id="KOG1102">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_547452_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQX0"/>
    <dbReference type="OMA" id="KYENEFH"/>
    <dbReference type="OrthoDB" id="4085843at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23436">
        <property type="entry name" value="RabGap-TBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="262"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="398"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="516" mass="59728" checksum="AB389B5993E9F82D" modified="2011-11-16" version="1">MSDQPLPQEQVAQDTQDSLTSVPIDDTASEQPNNVSSSDLPPPLPPRKDSPHIIQPLSFTLSDGISKISAVRQVEIYSSPQFHETSDLFVNNTLGNDTIRAKIDVLKGESDNEDIQYWVGIVEDYSNQFLKGNEGIDQVEQKVISGIPNEVRYLVYLKTLQVRYKLSSKETFSSLLNKARHANGSYEYIDKSTQDSNAKDLLIILDYLVNEVGSKAEHTRVNLTKFVINSSAVINQIQEFSPEERLYVLLKLNKLYQNLIKEEFFYKVNRTLEDELTDVFKHITLQGINLNSTYKEIIGNFFNKYFEDFSINLKILDFVIFEGFDFILRLIVWAVSCNKEKILQIDGDELLEFINSKELFSGPVNFQDILNIQPEIIKYENEFHLIHANSFNSNRNELTNLREVNDDLMIKVNELKQKIDTLQTTHQEIMNQSKEYKEQLSTAQQVNEKLTQEKQELNQKYEELTMKENLQNTIKANKEFYQRNNELQQQVDEVREKIEEMKVKLSKVSPVPVEGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQZ1</accession>
    <name>G3AQZ1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_56457</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376431.1">
        <property type="nucleotide sequence ID" value="XM_007376369.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQZ1"/>
    <dbReference type="STRING" id="619300.G3AQZ1"/>
    <dbReference type="GeneID" id="18872035"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56457"/>
    <dbReference type="eggNOG" id="ENOG502RPXV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_526878_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQZ1"/>
    <dbReference type="OMA" id="STQWIES"/>
    <dbReference type="OrthoDB" id="4093016at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="463"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="503" mass="58231" checksum="F398352F572F0583" modified="2011-11-16" version="1">MPLTSDKFDIGFLDVNQLASTFTKFNSFQNKNAHQQERTVIALRALELMDTYQAIHPCGHDMISHLKYFGVKSKYSAVWAREVERQSEEVLTVGKDKSSNIHKESVDSMNPEECHELFDFIKKCVIILLIERKYSEAHYFLNSYATVVQFSTQPKSADELKNNRYVEYLFLQYSANVFQTIWFPVDEQTGNSSLDKVISSSSFKPLETIYKKISTYYSKNEAVFSTRQNEGNYDELHYYWMIKMLYMAMLFKQFKFIEFYDEFYSLFLNYESKIQVSGLNPLQFLIKGDSILKSNLLLMFGIVSIFLKPACSLSFMNDDIIIDLFNENESSLEFLFYNQVLIPLSRRDLFTVQTTLHDQLFLDRLYAYLENSLPTGAASKYSPTHTFIEYMWKIIDFKNFLVIMSVTKQITRTRVIELLGYDLADSEVVNQVSNNLIVLISALGLGNGGIQYLASSELFIHTSPNKTAESVELQEDIEELNNEVKGMTLSSMMTSILIEKFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR76</accession>
    <name>G3AR76_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dynamin-related GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_139974</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376029.1">
        <property type="nucleotide sequence ID" value="XM_007375967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR76"/>
    <dbReference type="FunCoup" id="G3AR76">
        <property type="interactions" value="1350"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR76"/>
    <dbReference type="GeneID" id="18870257"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139974"/>
    <dbReference type="eggNOG" id="KOG0446">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008964_5_0_1"/>
    <dbReference type="InParanoid" id="G3AR76"/>
    <dbReference type="OMA" id="KICHNCG"/>
    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034643">
        <property type="term" value="P:establishment of mitochondrion localization, microtubule-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015886">
        <property type="term" value="P:heme transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097753">
        <property type="term" value="P:membrane bending"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090149">
        <property type="term" value="P:mitochondrial membrane fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140572">
        <property type="term" value="P:vacuole fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016050">
        <property type="term" value="P:vesicle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF235">
        <property type="entry name" value="DYNAMIN-RELATED PROTEIN DNM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00302">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="6">
        <location>
            <begin position="25"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="5">
        <location>
            <begin position="763"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="79"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="609"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="80"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="615"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="850" mass="95935" checksum="C4C75CE4539919B4" modified="2011-11-16" version="1">MSSLQDLIPVVNKLQDIITTTQLADIDLPILAVVGSQSCGKSSVLENIVGKDFLPRGTGIVTRRPLVLQLINISDEENDSNTTDFYSTSAPVSDEDGSHHHRGYDRQHPQIPNGHFSYSTDDVGSVNLEDHIRNMSNGKTTRSSREWGEFLHVPNKRFYDFHDIRREIEHETMRIAGQNKGISRLPINLKIYSPNVLNLTLVDLPGLTKIPIGDQPTDIEKQTRNLILEYISNPNCIILAVSPANVDLVNSESLKLARQVDPMGKRTVGILTKLDLMDQGTNALDILKGNVYPLKLGFIGIVNRSQQDISENKPLDESLNAEQRFFLNHPAYKAMASKCGTKYLSVTLNKILMAHIRERLPDIKAKLNTLRGQTEQELASYGDEFSEGEGEARGAKVLTLMTKFAQAFISSIEGTASSAAFNDVSTKELCGGARIYYIYNEIFGSQLASINPTHNLSILDIRTAIRNSTGPRPSLFVPELAFDLLVKPQIKLLQDPSRHCVELVYEELMKIVHNVCSSAIIGPELTRYPKLQSKLIEVVSDLLRERLGPTIKYVESLIEIHRAYINTNHPSFLGAAKAMSIVVSERQKQKQLESDSKLRLASERILSKNIKQSGSDDEEEEESDEPTDINQEIKSVDDVIPSKHKHKKSVKEFTPPVNSNNPDSYLNYFLGKDPIVHQQHLQQQAQLHPVPFKYPVHQEQVLQFNTNYIPSHNSEFNSNQLSPDSHLSDQFNSKMVMSDSIDDGSFTSDETINELTEREQLECELIRRLIISYFGIVREMIQDQVPKSIMCLLVNYIKQHIQNRLVVKLYNDNLFDELLKEDEAIQAEREKCLELLKTYKEASRIISDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARB0</accession>
    <name>G3ARB0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">NAD(+) kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_62318</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376495.1">
        <property type="nucleotide sequence ID" value="XM_007376433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARB0"/>
    <dbReference type="STRING" id="619300.G3ARB0"/>
    <dbReference type="GeneID" id="18874269"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62318"/>
    <dbReference type="eggNOG" id="KOG2178">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082194_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARB0"/>
    <dbReference type="OMA" id="HPHARIS"/>
    <dbReference type="OrthoDB" id="24581at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.30:FF:000009">
        <property type="entry name" value="Poly(P)/ATP NAD kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF0">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="218"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="32845" checksum="60DAAA960BCA819B" modified="2011-11-16" version="1">MNHCIDSGVKANLRMRFTCRVHGNDGKLICEQQVLNELVVDRGPSPYVTQLELYGDGSLLTVAQADGLIIATPTGSTAYSLSAGGSLVHPGVSAISVTPICPHTLSFRPILLPDGMFLKVKVPDTSRATAWASFDGKVRTELKKGFYVTIQASPFPFPTVTSSKTEYIDSVSRNLHWNVREQQKPFSSYLTPENQKRLERHDKSDMENFSSIQTTNEVHADSDNEEAFDINYSDDDGENEKEEEIEHERESSDEDFAFIPADSGTSTPMSSSYNNIDDRCCFAHPHARISLNQRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARK5</accession>
    <name>G3ARK5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Wings apart-like protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_51346</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376104.1">
        <property type="nucleotide sequence ID" value="XM_007376042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARK5"/>
    <dbReference type="GeneID" id="18871547"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51346"/>
    <dbReference type="HOGENOM" id="CLU_484985_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARK5"/>
    <dbReference type="OMA" id="ANEMSIM"/>
    <dbReference type="OrthoDB" id="10518225at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="562" mass="64186" checksum="ABE7E67F235AAEDF" modified="2011-11-16" version="1">MATYGNRKRNKRVRLSTSSTSFSDDLFSNDEIPSPSESFNKSPKKFISVRTTLEKDTSVKNLTQTLLNIADPPKEEKKVPKEKSKVKRKPKSVPRSTTKKSKPNKIIRSNAWDDLFNSIEEQPVIILANEQSSASEESETETNEVSFEIDFDSICKETTPTQPEPKRIVPKPQPKQKTYASDRTYLLDDNVELHVKQELTNEISSRHDFDNCEAILNVNDLRSSSNGMNTLYYILDGLSGTRSVLVSSLLELLVLVQGKISQSDLNKVVLAVKEIRWSDDIVVKLVSSVCYRVCTNHKEVLAQLESVLDKVILGLSTVIEITKFSRVNQNLAKPYLEPCFDIGFLREIQKQNLVNWPVILAILELLESSQRNNTDLLEILEVYFQDSKLIGLDVEKLTPLFEESFLRLDQDKNTEFDSRIIRVFIVLTTNYEKKVVQEIYDSKWTHIIFGFLDRTYRDANPESLSLCLLLLGLLMNLVEWDLIEVDVPIIANNMAQLEALDNQHNNEIKSYIIGYNAIVLSHLQIKYSSQLTINRSKLKTWLTQFQPTAAISGKVYILLQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARX3</accession>
    <name>G3ARX3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">precorrin-2 dehydrogenase</fullName>
            <ecNumber evidence="2">1.3.1.76</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_139882</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.76" evidence="2"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376600.1">
        <property type="nucleotide sequence ID" value="XM_007376538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARX3"/>
    <dbReference type="FunCoup" id="G3ARX3">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARX3"/>
    <dbReference type="GeneID" id="18870248"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139882"/>
    <dbReference type="eggNOG" id="ENOG502RYIW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_8_5_1"/>
    <dbReference type="InParanoid" id="G3ARX3"/>
    <dbReference type="OMA" id="PTGCKLT"/>
    <dbReference type="OrthoDB" id="1721126at2759"/>
    <dbReference type="UniPathway" id="UPA00262">
        <property type="reaction ID" value="UER00222"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043115">
        <property type="term" value="F:precorrin-2 dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.110">
        <property type="entry name" value="Siroheme synthase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3280.10">
        <property type="entry name" value="Siroheme synthase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028161">
        <property type="entry name" value="Met8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028162">
        <property type="entry name" value="Met8_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028281">
        <property type="entry name" value="Sirohaem_synthase_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330">
        <property type="entry name" value="SIROHEME BIOSYNTHESIS PROTEIN MET8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330:SF1">
        <property type="entry name" value="SIROHEME BIOSYNTHESIS PROTEIN MET8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13241">
        <property type="entry name" value="NAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14823">
        <property type="entry name" value="Sirohm_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14824">
        <property type="entry name" value="Sirohm_synth_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75615">
        <property type="entry name" value="Siroheme synthase middle domains-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Siroheme synthase central" evidence="8">
        <location>
            <begin position="179"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Siroheme biosynthesis protein Met8 C-terminal" evidence="7">
        <location>
            <begin position="210"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="302" mass="34916" checksum="D870271DF2F94674" modified="2011-11-16" version="1">MSHEIARHSRIPQLRHPKESQSQQIPQTRSLLLAWQVRNKHCLVIGAGDVALSRIEHLIRAQAKVTVVTGDSKIHPEITKLNHQGKLFSLIARNYHSNDLTLYENISISKDIDLITKEDYEKISHSVYNETFAIVCCCIDDYELSTKIYYQSKLLRLPVNIADKPNLCDFYFGSMINQDNLQIMISTNGKSPRLSRLIKDNIAKELQDVDFNQAIENLGLIRSRLREKVLENDDLVSIDARMTWIKTLTDFFSLKQWAELKLIPESSAPPYDNQFQYVDNIIAYFPSYPPEDYDEFVKAVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS53</accession>
    <name>G3AS53_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_68229</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377045.1">
        <property type="nucleotide sequence ID" value="XM_007376983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS53"/>
    <dbReference type="SMR" id="G3AS53"/>
    <dbReference type="STRING" id="619300.G3AS53"/>
    <dbReference type="GeneID" id="18875205"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68229"/>
    <dbReference type="eggNOG" id="KOG1502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_9_2_1"/>
    <dbReference type="InParanoid" id="G3AS53"/>
    <dbReference type="OMA" id="NYVKCSA"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="332" mass="36653" checksum="F4F7560D6C78392B" modified="2011-11-16" version="1">MSPTSVFVSGANGFVAQHVIKLLLAKGYAVVGSVRSATKGDELKELVKSDKFSYEIISELGDKPGFDAALKKHPEVTGFMHIASPVGFTATDIEKELIRPAVDGTVNALKAITEYAPQINKVVITGSVVSVFGFGPHFDGNKVYTEDDWNPITYEESLSNPMFGYFVSKKFAELAALEFIEKEKPNFDTSFVIPCYVFGPQAYGVKNKKKLNLSNEIINTVVKLGKDDKIESFGSVFVDVRDVARAHLVAFENKRAVPKRLLLISEKFSLDSIAHIINEKFPDSSVPKGDISKDVELEKSIQKFDNSKTKEILGFDFIPLEKTVEDTVTQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASD5</accession>
    <name>G3ASD5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62547</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376709.1">
        <property type="nucleotide sequence ID" value="XM_007376647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASD5"/>
    <dbReference type="STRING" id="619300.G3ASD5"/>
    <dbReference type="GeneID" id="18874370"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62547"/>
    <dbReference type="eggNOG" id="ENOG502R6C6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_946636_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASD5"/>
    <dbReference type="OrthoDB" id="421038at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="297" mass="34119" checksum="31AB8FD1E12376A6" modified="2011-11-16" version="1">MEQPASSTSSIGGLRLLSKKKTIPRAKRCLEKFVLRTQSGWSIPFKQGHLSLVCEYFDVIKELHLVNFIIDDMKPINIDGVQIQKLIIESCIYTNSFKKSRKPQPNSIFNNVTTLELKSIMHDNGLSLIDLVRLNNTSLNHLIMDTSSSMFYTNTPSGNTEFNFTRFNPFFNLLCSGYGKLTTLTLTNFGLFDYFIHDVVHEDSDSWVEPPTNNFETFMKYVSQINRLVIVLRKTPSMVKTCIKCGFTEQQSDKNIDSLTPGEWKVFLKPLELNSDNSLKIFNYRNKLLYSSSKYNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASK5</accession>
    <name>G3ASK5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nucleoside transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_62558</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376724.1">
        <property type="nucleotide sequence ID" value="XM_007376662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASK5"/>
    <dbReference type="FunCoup" id="G3ASK5">
        <property type="interactions" value="273"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASK5"/>
    <dbReference type="GeneID" id="18874377"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62558"/>
    <dbReference type="eggNOG" id="KOG1479">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021611_3_0_1"/>
    <dbReference type="InParanoid" id="G3ASK5"/>
    <dbReference type="OMA" id="GSPWTTK"/>
    <dbReference type="OrthoDB" id="46396at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034257">
        <property type="term" value="F:nicotinamide riboside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002259">
        <property type="entry name" value="Eqnu_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332:SF88">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01733">
        <property type="entry name" value="Nucleoside_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016379">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="285"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="357"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="43782" checksum="5450F81E857D61DF" modified="2011-11-16" version="1" fragment="single">MTDHRSTESQLETSTTHQHDPIIFRLGKLHLRLSSLKYFTFTIIGIALLWPWNAFLSASAYYAERFGHSPSLIKIYSSTMMSVSTITSMLYNFYLSQVQTGVNYNFRVNTGLWITISVFLIMGISCISDLFIRMHDIAFFVLLMFMVLLSAMATCLSQNGTMAIVNVMGSIYANAVMVGQAVAGVLPACALIISILLVGDRIDAKQEYVDKNYGVFLYYITASLISIVSILLLYWVTYHKNEIAYQRLNSVFSGGEEVEEEDLVEPVHAQKAYVSFWTLWSKLKLIVMTIFLTFGITLIFPVFASTVESTHTDSPNKFFHKSIFIPFIYFVWNLGDLLGRILCGYPRLRMLIKDPKVLILYSISRLVFIPLFLTCNIHTYTASGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AST0</accession>
    <name>G3AST0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_142523</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377178.1">
        <property type="nucleotide sequence ID" value="XM_007377116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AST0"/>
    <dbReference type="STRING" id="619300.G3AST0"/>
    <dbReference type="GeneID" id="18870456"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142523"/>
    <dbReference type="eggNOG" id="KOG1674">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063721_0_0_1"/>
    <dbReference type="InParanoid" id="G3AST0"/>
    <dbReference type="OMA" id="ENWCNIL"/>
    <dbReference type="OrthoDB" id="4138492at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF36">
        <property type="entry name" value="PHO85 CYCLIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="1">
        <location>
            <begin position="92"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="296" mass="34163" checksum="46BC9E68F5691825" modified="2011-11-16" version="1">MDTPISPKTKTVEPVATIARNNSTASLLTPSSTPTTKQDPTTPTVSTIPVSPINSYYYTSHLTKSQFNSILVNCTINLIKILYSESKINETNLSIFIVEILKRSKTSIQTLQLACFYLTKLINKKEKITITDPKKLFLGLVILASKFNQDYNYSFKSWLKICGLESSSTKVIKELEFKCLQMLQFELSLNGDKYENWCNVLMIFGYDFIKCHLIDSNNSNIMWETKQETIEVKLTKWTKFFADFNEDNLNILQIKFSQYFINQLNKKIFIVKDEEPKLLKRARVDDVPCPKKQRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASZ8</accession>
    <name>G3ASZ8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_51980</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376813.1">
        <property type="nucleotide sequence ID" value="XM_007376751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASZ8"/>
    <dbReference type="STRING" id="619300.G3ASZ8"/>
    <dbReference type="GeneID" id="18871615"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51980"/>
    <dbReference type="eggNOG" id="KOG1205">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_10_1"/>
    <dbReference type="InParanoid" id="G3ASZ8"/>
    <dbReference type="OMA" id="IVLCTQR"/>
    <dbReference type="OrthoDB" id="6251714at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000047">
        <property type="entry name" value="NADP-dependent L-serine/L-allo-threonine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42901">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42901:SF1">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="265" mass="28835" checksum="03ACF2589D00DBE0" modified="2011-11-16" version="1">MSYGIKAAERLANKSILITGASSGIGAATAREFAHASRGQISLILGARRQERLQQLSQSLTTEYPNIKVHTAFLDVTIKDSIREFVNGIPGTPDVLVNNSGKALGRANVGEITDEDIRGMMETNVVGLINMTQEILPKMKQRNTGTIFNVGSIAGWDGYVGGSVYCASKAAVRYFTDSLRKELIDTKVRVLEVNPGAVLTEFSLVRCKGDEQAADKVYEGTEPLTAEDIAEIIVFGSSRRDNTVIAETIVYPQHQASPVHIYRKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATD6</accession>
    <name>G3ATD6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62810</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376932.1">
        <property type="nucleotide sequence ID" value="XM_007376870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATD6"/>
    <dbReference type="FunCoup" id="G3ATD6">
        <property type="interactions" value="196"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATD6"/>
    <dbReference type="GeneID" id="18874474"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62810"/>
    <dbReference type="eggNOG" id="KOG1674">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018149_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATD6"/>
    <dbReference type="OMA" id="YINRAKP"/>
    <dbReference type="OrthoDB" id="10250320at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012104">
        <property type="entry name" value="PHO85_cyclin_1/2/9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF10">
        <property type="entry name" value="PHO85 CYCLIN-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016511">
        <property type="entry name" value="Cyclin_Pcl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="1">
        <location>
            <begin position="46"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="291" mass="33115" checksum="A93A646FC85B6DFA" modified="2011-11-16" version="1">MSDREALAIFSRQRVSSEMVNFLVATTNSIIQIKPAKPVSTKHHNQLIITPHNNTISLTDFIHNLINYSNVQVPTLMATLVYLNKLRNLLPANAIGMETTRHRIFLSTLILAAKSLNDSSPLNKHWTKYTDGLLSQQEVNMAERELLSLLKWNINIKHQELIIALQPFLEPIKQSLLRERQRESEQRANYYKLLQAQSKSNWSIRTSKSSVSSNYSLDSSYSVSSNLSLARGMPHSASSSSIISENEEYDLVPKRVPLSNKSVNVLHQQQHPPVKMKRSTHSFNLLSSRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATF5</accession>
    <name>G3ATF5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WD40 repeat-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_142494</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376951.1">
        <property type="nucleotide sequence ID" value="XM_007376889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATF5"/>
    <dbReference type="FunCoup" id="G3ATF5">
        <property type="interactions" value="871"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATF5"/>
    <dbReference type="GeneID" id="18870453"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142494"/>
    <dbReference type="eggNOG" id="KOG0290">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013694_4_0_1"/>
    <dbReference type="InParanoid" id="G3ATF5"/>
    <dbReference type="OMA" id="TIAMDAC"/>
    <dbReference type="OrthoDB" id="1284551at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045159">
        <property type="entry name" value="DCAF7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19919">
        <property type="entry name" value="WD REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="412"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="218"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="54044" checksum="C8C22DCA19454D3A" modified="2011-11-16" version="1">MDWYNNRSKRSSISTGAPSYSPASYGILSTSLHSPFSHNQSRFTSGTTPVPKTPPLSSTSSPSLMTTTTPLERNSYYFSQLPLYTSDWQYVANSESDCIALGSYKEGFTNKLEIIHGVSYEQEFKNNDDVMSPTAGYDDDVVQGFYFSRVAETSVDYPITHLQWDPMMASNGGSERLAASSEVLRLYKVNKEEGYDSNNNHHNNYSLTQTHILANNTASSCTSSSNSLGNNGSSSKNDDINTYPPITSFDWNKTDPSILITSSVDTTCTVWDLHRSHPRDETTDTATVKTQLIAHDSEVFDVKCIHKSTNVFASVGNDGSMRVFDLRSLEHSTIIYEPPTMTATSSSSSSNPASSTFNSKALICLSTSNIDQHHLATVGINSNLVIIIDMRMPGIPVATLDGSFGGINHASINSIQWHPTSNYLLTGGDDCQALVWDCNNLTPKSGSSSSSTGVVLDTPVLAYEEDLEVNNVCWRKDKGDWMGVISGKGFQAVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATS3</accession>
    <name>G3ATS3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_63145</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377269.1">
        <property type="nucleotide sequence ID" value="XM_007377207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATS3"/>
    <dbReference type="FunCoup" id="G3ATS3">
        <property type="interactions" value="195"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATS3"/>
    <dbReference type="GeneID" id="18874616"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63145"/>
    <dbReference type="eggNOG" id="KOG0756">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052717_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATS3"/>
    <dbReference type="OMA" id="VEMTKHK"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005371">
        <property type="term" value="F:tricarboxylate secondary active transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015742">
        <property type="term" value="P:alpha-ketoglutarate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006843">
        <property type="term" value="P:mitochondrial citrate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000053">
        <property type="entry name" value="Mitochondrial DNA replication protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000085">
        <property type="entry name" value="Tricarboxylate carrier, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053017">
        <property type="entry name" value="Mito_Cit/Oxoglu_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46982">
        <property type="entry name" value="CITRATE/OXOGLUTARATE CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46982:SF1">
        <property type="entry name" value="CITRATE_OXOGLUTARATE CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="7">
        <location>
            <begin position="99"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="7">
        <location>
            <begin position="204"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW30298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="298" mass="32412" checksum="53E95A680F53719E" modified="2011-11-16" version="1">MTQEIKKQPVNFSNILLGAGLNFAEVTTLGQPLEVTKTTMAANRSLTMPQAVKFVWSRGGVLGFYQGLIPWAWIEASTKGAVLLFVSAEAEYRFKTMGVSPFVAGMGGGITGGLAQAYLTMGFCTCMKTVEITRAKQAATGAATQTSFQVFKEIYKNEGIRGINKGVNAVAIRQMTNWGSRFGFSRLAEEAIRKFSGKSENQKLSAWEKIAGSVIGGGLSAWNQPIEVIRVEMQSKTNDPNRPKNLSVVGAMKYIYQGHGLKGLYRGVTPRIGLGVWQTVFMVAFGDIFKKMLNADGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATW5</accession>
    <name>G3ATW5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DUF747-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_57145</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAPT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377312.1">
        <property type="nucleotide sequence ID" value="XM_007377250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATW5"/>
    <dbReference type="FunCoup" id="G3ATW5">
        <property type="interactions" value="278"/>
    </dbReference>
    <dbReference type="GeneID" id="18872097"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003655_1_1_1"/>
    <dbReference type="InParanoid" id="G3ATW5"/>
    <dbReference type="OMA" id="YHDVRGQ"/>
    <dbReference type="OrthoDB" id="5376140at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008010">
        <property type="entry name" value="Tatp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317:SF4">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF05346">
        <property type="entry name" value="DUF747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="428"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="583" mass="66413" checksum="9AB157F49F01A815" modified="2011-11-16" version="1">MDKNNKQDSPSQASPLPTPDSSKQSTPTTSTPPLPQDHSRSRKNSLLDFLVPITLLLLELNLSNEPKDEDDKEQLQLATNSFDEVLNMCKIPFYLERYMLFGLLVCLNTFLTLFTLAPLKIVIVTIQALFGFVFKRKSSSWSSFQLVKRDLITFSLIPISIYILTTLNLDISRMYHDIRGQTDIKLYVMFGVLEVAEKLCSSLGQDILNILYQSNQSIPRFIIFYIIGMFYLSFHAYILIYQTVSLNVAVNSYSNALMTLLLSNQFSELKGSVFKKFEREGLFQITMADLSERFQLSLMLSIIGLRNLSQLSINGTLIPNSFSKNWNIWFGALFGPGFIVIGSEVFVDWLKHCYISKFNRFRPRIYRNFLYVSCLDFLEMFHHSNHSREFSDYLALTRRIGLPLLATVVCFLKMTLVDFKTIFIGPSILSSAGLILATYLTLLLVRVILGLVIFKTASILVIKHKHHQAELKSKTVPDIVVPTPIKQTTILERTPSPSDDSYSHLSISATFLRGSPNTEPSTINSKTRAHLYDADEKIPPTLEETRNKKLLKGPLHESKLEKDAGDEGLSQVMRYKMSSKRIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU32</accession>
    <name>G3AU32_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_63257</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377378.1">
        <property type="nucleotide sequence ID" value="XM_007377316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU32"/>
    <dbReference type="FunCoup" id="G3AU32">
        <property type="interactions" value="519"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU32"/>
    <dbReference type="GeneID" id="18874661"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63257"/>
    <dbReference type="eggNOG" id="KOG2071">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015606_1_0_1"/>
    <dbReference type="InParanoid" id="G3AU32"/>
    <dbReference type="OMA" id="ARSDFAN"/>
    <dbReference type="OrthoDB" id="2129491at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005849">
        <property type="term" value="C:mRNA cleavage factor complex"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:mRNA 3'-end processing"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="mRNA cleavage factor complex component Pcf11"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045154">
        <property type="entry name" value="PCF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054127">
        <property type="entry name" value="Pcf11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047415">
        <property type="entry name" value="Pcf11_CID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021605">
        <property type="entry name" value="Pcf11_Clp1-ID"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921:SF3">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX 2 PROTEIN PCF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21936">
        <property type="entry name" value="Pcf11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11526">
        <property type="entry name" value="Pfc11_Clp1_ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="2">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="239"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="542" mass="60712" checksum="ECCAD2002A149839" modified="2011-11-16" version="1">MSDILESYQQTLSELTFNSRPIIETLTTIAKENPTVADGIIDLITQRIRRAIPQHKLFALYLLDSICKTVGNPYNILVGYDVYNLFIDVWKVVDEATRTRLVALFETWKISKVRGGGESLFPRDQLDKVEGFLKKVGKLTSAPTQSQSQKSQSGPVLSNSKLISDINQLIPIFEAKLKRTNDSKLNDKFRALNQLKIILTNQQMKPQELQAVQNQLNAIKEQELSPAPPSLPPIPPKPVIHQQHQQQQQQPPQPLQPIVNASQIFNDLILSGLVKKEQEPIPGSKPNYTLVFPTIKYVPKTEIPGVDVLADILNPNSNIQRSEYQKLKFGELVQISKAISGDDNSLQKFINTNKPSHSSVQLLYNSKASKCSICGKRFSTDQEGSTKKRLHLDWHFRINKKLTGTGSGATGTGGGVVSNVQSRSWYLDDYDWANFKDEQLLEFATPEVAQTATINSNIPSGVVPYVVVPSADTNMNNKCLICLEQVKASYNDEIGEWCWMGCIKSPNDSKNLRKIVHITCFNDRKRATDTEDGNTRKRGKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU46</accession>
    <name>G3AU46_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Choline transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_57208</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377393.1">
        <property type="nucleotide sequence ID" value="XM_007377331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU46"/>
    <dbReference type="FunCoup" id="G3AU46">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="GeneID" id="18872108"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57208"/>
    <dbReference type="eggNOG" id="KOG1289">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004495_2_4_1"/>
    <dbReference type="InParanoid" id="G3AU46"/>
    <dbReference type="OMA" id="GIPWIAF"/>
    <dbReference type="OrthoDB" id="2417308at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015101">
        <property type="term" value="F:organic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000046">
        <property type="entry name" value="Amino-acid permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF7">
        <property type="entry name" value="CHOLINE TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="382"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="443"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="477"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="539" mass="59513" checksum="E5A2A477FB932422" modified="2011-11-16" version="1">MSDSTQVSNTHADEAKRVRLLEDARDNLEFAKESGYKPELKRGFSLISLLGVGFGLTNSWFGVSASLITAIKSGGPMMIIYGVVIMTFIGMCTACTLGELASALPNSGGQYYWTQKLAPKKYARFMSYLCGSFAWAGAVFTSASVTVAIATGAVGMYLISYPEKEFQKWQVFIAYELANLFLVGFNCFEKPLPHISKASLYISLSSFVIITITVLAMNNEGFRSAKYVFVDFTNDTGWHVGAIAFFVGMINPNWSFSCLDAATHIAEESSRPRVDVPKAVIGTVIIGFLTSFPYSIAVFFCLKDLPAIQNSNTGVPIMDLYYQATKSKAAAVGLEFLVFFTAIGCNINCQTWASRLAWSFSRDNGLPGSKWWSKVNKKTGSVVNAQIMSCCWSGIIGFIYLGSDTAFNSILVCCLLFLQISYVLCVSCLIFKRNQIKPGPFWLGKLGLFCNVVVICWFLFTFTFYNFPAVVPVNAGNMNYSCVVIGLYFTYCVLDWNLRAKKSFIGTEEQEGNVELTNELSREMSQIEAVLSNTGEKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUC3</accession>
    <name>G3AUC3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribonuclease P protein subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_157605</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377470.1">
        <property type="nucleotide sequence ID" value="XM_007377408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUC3"/>
    <dbReference type="FunCoup" id="G3AUC3">
        <property type="interactions" value="225"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUC3"/>
    <dbReference type="GeneID" id="18871089"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157605"/>
    <dbReference type="eggNOG" id="KOG4046">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080488_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUC3"/>
    <dbReference type="OMA" id="NMIGIEG"/>
    <dbReference type="OrthoDB" id="124041at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="entry name" value="Ribonuclease P/MRP, subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016848">
        <property type="entry name" value="RNase_P/MRP_Rpp29-subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023534">
        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027081">
        <property type="entry name" value="RNase_P/MRP_p29_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00538">
        <property type="entry name" value="POP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="Rof/RNase P subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR027081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="258" mass="29701" checksum="02D17FE5BDB7F5B1" modified="2011-11-16" version="1">MNSNELEKHLLTRCYDSQNRIVELLEQRYATNGDQKSSIVFTKSESTTTAKLFSNDTPTISRARLQTTRKSLKAYISSTLANQRKLNKRIKQYKRKSHTTSPFPRDKLIEEHRIPQITQFIHMNKLWQKYMQDLLFPNGQIQALPNILPKLSSADFNGCLVKVVQSRNRNCVGMQGIVVWDAQHSFVVCVPGTDEDIGGFKVIPKQHTLFAFDLILPSKEEQEEEECIPFTIIGSRFEVRSVDRSGKKFKNHSIDDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUF2</accession>
    <name>G3AUF2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_57283</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377500.1">
        <property type="nucleotide sequence ID" value="XM_007377438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUF2"/>
    <dbReference type="GeneID" id="18872110"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57283"/>
    <dbReference type="eggNOG" id="ENOG502RPID">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109474_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUF2"/>
    <dbReference type="OMA" id="DNEMPND"/>
    <dbReference type="OrthoDB" id="3991133at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="216" mass="26387" checksum="C59F45A9A762B044" modified="2011-11-16" version="1">MYKPKFQFRPNTTGLIINKPTIDLTKVRNQIVQIPTFKRKLLYLYKQFYKLCDFQYHEQHDNINKYQTLLRRNFSHISFNNRRNVILSTTEDLNEKELVERMLNTLAFVFNATVARDDPHIFDEIHNHDDLKKTNIENMEANILNTILRMDSTKPDVIKYDFQYNWWGRFRNDVKKVQDWEKVTRKQLRSMNLEWMTFYQHETTVMRLNENLKLCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUN0</accession>
    <name>G3AUN0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_52658</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377557.1">
        <property type="nucleotide sequence ID" value="XM_007377495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUN0"/>
    <dbReference type="GeneID" id="18871703"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52658"/>
    <dbReference type="HOGENOM" id="CLU_1826506_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUN0"/>
    <dbReference type="OrthoDB" id="10491354at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="141" mass="15968" checksum="4266F3A513349B09" modified="2011-11-16" version="1">MIYINSNELLDYYLNSFSHSSSTKLPEDLINNELLISSKIEQILSRIETNSGGISEESEEFETVDINDIIVDEDAFLSHVANTLSSGVDEAVAKRFTEEQKLHRLTFLKEIKEKGLSLRHAIDRSESSKESKDSSGTFMNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUP1</accession>
    <name>G3AUP1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_57336</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377568.1">
        <property type="nucleotide sequence ID" value="XM_007377506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUP1"/>
    <dbReference type="FunCoup" id="G3AUP1">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUP1"/>
    <dbReference type="GeneID" id="18872112"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57336"/>
    <dbReference type="eggNOG" id="KOG1752">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026126_7_2_1"/>
    <dbReference type="InParanoid" id="G3AUP1"/>
    <dbReference type="OMA" id="RKWVPTI"/>
    <dbReference type="OrthoDB" id="418495at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015038">
        <property type="term" value="F:glutathione disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694:SF18">
        <property type="entry name" value="GLUTAREDOXIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="1">
        <location>
            <begin position="21"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="111" mass="13284" checksum="4C604A9E5658313C" modified="2011-11-16" version="1">MASKKYLERAQELINTHPYLMLSKSWCPDCHYTYRIWDRYHVADKVHIIELDKFADQNEATELEKAFTEISGRKWVPTIFFNGKRLGTEADLKKWDSEDKLDEIFKNEKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV43</accession>
    <name>G3AV43_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63734</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377883.1">
        <property type="nucleotide sequence ID" value="XM_007377821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV43"/>
    <dbReference type="GeneID" id="18874887"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63734"/>
    <dbReference type="HOGENOM" id="CLU_954950_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV43"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="292" mass="33716" checksum="89E6B4C827D0085C" modified="2011-11-16" version="1" fragment="single">MELQGIVSDMNRNQITSAQLEQQYGANNVAIEITDSDEDYQYNSEDDALDSEEDGLEFEEYEYDSEDDAIDLQEGFLDSEDDGFDLEQDYLDQNVDQQPDPFAAHDLDSYQEQQRDIVASHDPVIEINQQEGIIRLMNSVIRIFCPQIWTLFNDNLGLQPVAHETCLIIIIMIVVGFILRIVLFYDFKTSIETIYTKLYPMCEVMAYKINYPDSVVRSCYHKYLSDGAPLAKINIYWSSMNKALLNCLEEGNREFDVSPPLPRIEIMFPKKILEHALRMCFLENSKCVGSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV66</accession>
    <name>G3AV66_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peroxisomal membrane protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_157987</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377635.1">
        <property type="nucleotide sequence ID" value="XM_007377573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV66"/>
    <dbReference type="STRING" id="619300.G3AV66"/>
    <dbReference type="GeneID" id="18871110"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157987"/>
    <dbReference type="eggNOG" id="ENOG502RXMH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054132_1_0_1"/>
    <dbReference type="InParanoid" id="G3AV66"/>
    <dbReference type="OMA" id="VMVFLFR"/>
    <dbReference type="OrthoDB" id="39659at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019531">
        <property type="entry name" value="Pmp4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15460">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15460:SF3">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF013674">
        <property type="entry name" value="PXMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW29869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="26581" checksum="53418175108625EA" modified="2011-11-16" version="1">MSSILTNPLYRPLLDVLRSSRNGIVYGGKIRFSHALVINLLYRSGPLTPRLKSVLEATKNHAEVLASFAIIYKAAVIILKSEAFLGPKNMRLIKFLAGGFGSWIVYSQHFKFFHGGITHQITLYCFSRVLIAVGKILLDQYLACFQPSFRNYQGDLIKYRDLNNNQQKKLKGLIYDKSWKYFAVLTWALVMFIYDYQPQYLQSSLRHSMAYIYDVEMDSWSTWREFLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV82</accession>
    <name>G3AV82_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 16</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 16</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MED16</name>
        <name evidence="12" type="ORF">SPAPADRAFT_57394</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 16 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377651.1">
        <property type="nucleotide sequence ID" value="XM_007377589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV82"/>
    <dbReference type="FunCoup" id="G3AV82">
        <property type="interactions" value="268"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV82"/>
    <dbReference type="GeneID" id="18872115"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57394"/>
    <dbReference type="eggNOG" id="ENOG502QWAC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013428_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV82"/>
    <dbReference type="OMA" id="FDTTWLG"/>
    <dbReference type="OrthoDB" id="4139168at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048338">
        <property type="entry name" value="Mediator_Med16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048339">
        <property type="entry name" value="Mediator_Med16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021665">
        <property type="entry name" value="Mediator_Med16_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13224:SF6">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13224">
        <property type="entry name" value="THYROID HORMONE RECEPTOR-ASSOCIATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20719">
        <property type="entry name" value="Med16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11635">
        <property type="entry name" value="Med16_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med16 N-terminal" evidence="10">
        <location>
            <begin position="147"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator complex subunit 16 C-terminal" evidence="11">
        <location>
            <begin position="861"/>
            <end position="974"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF11635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW29885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="981" mass="109752" checksum="CAE3618D8CDD23F9" modified="2011-11-16" version="1">MDKQSLTNKHLASLTNTSKLISWSKNGFIAYSSPLASSTNNLYLTYLENINGKTWQLAPPQPINIKLENNFLPEISLVSWSSLNTDLAIADVYGNFYILLAGVGLLDDTTTTTTTTTGRTSPTTFSPSYELTSYNHMEMIYRDIINQDSKSTVNPGASIIAFKWMNIEKAQLMNKPATLVPDSNSPVPNSNNGQFAYTYGASSFPPHGATHPLATKQACVALRQNGQFMLYYQGEHKVEYHKLCVGLTDGISVITKASIGFANDKQIIITAYDSITDTISTYSVVIDWGFLVEAAKRQKVDQHYHTPKEAQKPVKMTVNRIHDMKPAVKFLDDEDDSDEDKMDVDNVKYTIGELVSVDVISANAEKDSKPSVLIGYAYTNQSDHITSIIYRYNITDKLDLVSDAFMELGARKGVVSPSTPTKKSVTLTLMDKITRSGQIRHINTVLNDFYMLIAYMDGKIDVIDLSTWKLVTHSTEQFPPTSISTIFDVGFSFPKIPHTNPLILAVSPNMTSLVYTEVYSDKPNLSLVMVEKTRHTGYSSRDLFTTSVGFAFRHAYTCYTNTCSDDLLVLIQSEIVRLRAKLSETKANSNVDVILDKFVESIITESHKAINFQLDGFSRESVDKLLSNPPLQKLLSLQLVLGEFLGTNSISSDIAWMILNLRSTSFGIMFSLSSIYRQISKKKPSEDSLQDSIARAECIVSLIGNMKWLIDLMVYLNQELLQLSLCKRDEDSKNSRVTIANSIVLPVILSKVPRLFIMYAISSIGKTHEILKKLHKDLNEANKLFTPMKESLNRYFTICDHAPLTLSLFENFLRECDALITKELSTRVTTPGVVLRLEQKLVCRGEITGEMSQIAPYIIERYAANINRELKASDLYFFDVDWLDIGIIRDQSRESSPVLVDSSTSKIAPRLRMQDGYIDALRKITIGGGEIRKCTRCRAVSLVSDPLVFDGSVGPGLWTMVFQRTCLCGSVWVNVNTSNRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV84</accession>
    <name>G3AV84_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_144711</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377653.1">
        <property type="nucleotide sequence ID" value="XM_007377591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV84"/>
    <dbReference type="STRING" id="619300.G3AV84"/>
    <dbReference type="GeneID" id="18870659"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144711"/>
    <dbReference type="eggNOG" id="KOG0590">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="InParanoid" id="G3AV84"/>
    <dbReference type="OMA" id="PFEDHIT"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF541">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SKS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="26"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW29887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="323" mass="37166" checksum="1AA3749181586882" modified="2011-11-16" version="1">MQSTVSTASSTYSVSTKPHPKFSRQDARLMALGDGVSGFVELFQHKSGFLYAVKTYHCKETYESRSEYKTRVLYEYHLLAKLDHRNIIKVFKYDVSLSGNTVKFYMDAGSANLAQLIRVIPKHKYNQNEMLCIWRQIVAGVNYLHDRGICHRDLKLENVVFDIDHFQIKIIDFATADEKEVCIGIVGSEIYLAPETYTSIKYHGTKVDVWSVGIILFYLWNRKFPWKSARWNDPEYKEFHAESARPESDQASTTIHLKQSVLQHLPVASHALTSQIFECNPETRSSIADFYKDTWFTSIGFCDGVNLCGIDHHINFNPDKVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVD8</accession>
    <name>G3AVD8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_73543</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377922.1">
        <property type="nucleotide sequence ID" value="XM_007377860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVD8"/>
    <dbReference type="FunCoup" id="G3AVD8">
        <property type="interactions" value="991"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVD8"/>
    <dbReference type="GeneID" id="18875489"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_73543"/>
    <dbReference type="eggNOG" id="KOG2002">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003008_0_1_1"/>
    <dbReference type="InParanoid" id="G3AVD8"/>
    <dbReference type="OMA" id="EHWLTIA"/>
    <dbReference type="OrthoDB" id="343875at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000791">
        <property type="term" value="C:euchromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990269">
        <property type="term" value="F:RNA polymerase II C-terminal domain phosphoserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045142">
        <property type="term" value="F:triplex DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901525">
        <property type="term" value="P:negative regulation of mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001209">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060260">
        <property type="term" value="P:regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090262">
        <property type="term" value="P:regulation of transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031126">
        <property type="term" value="P:sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001015">
        <property type="term" value="P:snoRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031101">
        <property type="entry name" value="Ctr9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14027">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN CTR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14027:SF2">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN CTR9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="374"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="779"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1010"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="933"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1010"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1028"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1064"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1100"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1124"/>
            <end position="1137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1137" mass="128184" checksum="6474FF17EB3F03DB" modified="2011-11-16" version="1">MAHPSTAPAVATTASSAVDSPAATATAAGAATSTVTTTDAVKLHNLDVPLSNGEIVQINLVNDLPEDHNDLIGFLEGEHCGKQYWITVASAYAKTNKLTEAMGIIDAALKLDYFGEEDKKSFQSFLVWLYFKFVSLGIEKDQYLNQASVEINKLAQRIKTDASTSTSNSTSNILSQAVLLLFNGQDDDALDIFDKILRIDQNNCFATLGKAQVILNKTKNYSLALKLYQQVLILNPLMKPDPRLGVGLCFWFLKDDKMALAAWERCLELDPENIKAKIFINLAKFHLAFNNSLSDEEFLENYKTCLVEVSKINAKAPQDATVLLPLASYYFSKGDYETVEKIIKKIVYNITGDDNLTKFSNFTKISKYNSNILSQCCTWLGRIQFTNSDFLQASKYFQEAIKLNDTNIVAKLGLGQAQYNRGSIEEAIMTFESILRSNMKCLEVNYSLGILYSKQSSRRKQEMAIQILERYVRLSNNRGLSSSKEDSSEFLLNKEPICLNAYLTLSKLYESSDVNQSLNYLNKAIESRKHISKPVPLEVYNNIGVFNFMKQNYDEASNNFQVALDQLDGAEFKSPDGDLLIDLPQDLKVSLTFNLARSKEISNKDDALKIYETLLQECPHYFSAKLRILFLNCILEEGGYTKQEIKTELEALMSSNSSNLEIRSFYGWFIKNFGKKLGLPADADTKHQKDTLVDYDSHDCYALISLANIYCIMARDAKGDNEKRKKYYIRAIELFTKVISVDPKNVYAAQGLAIANIENKESNKGLDILRKIRDSLNDISVYLNLGHVLTELKQYGKAIENYELALGRFTDGKDSKILSFLGRAWYLRAQSENNLSFYRRSLEYSKLAFECIKGTGSKSSIRFNIAYVHFQIAEFVTKQPVFQRKLEDIEAAITGLKEGIELLNELASDDEKHPPYPKEELRARANLGTTTLLNRLNVVFEETKASIAEVEQKLEVAKKLRQEEEEAKLEEEKQRVAALKEKEEKLAQERALLQEQAQQWAEEARMNVEVDEENDDDLFNEESAAADKKRKSKPAKGGDKKKQKKTKKRAKKNVVDDSDEEEAASSSGGESDAEENTNNNGTQKGGKKSKKQFKSNEFINDSDDLDDDLFGGDDAEEEAKLSQEEEEEEEANNGDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVL8</accession>
    <name>G3AVL8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_63589</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377733.1">
        <property type="nucleotide sequence ID" value="XM_007377671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVL8"/>
    <dbReference type="STRING" id="619300.G3AVL8"/>
    <dbReference type="GeneID" id="18874820"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63589"/>
    <dbReference type="eggNOG" id="ENOG502QQIE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012066_3_2_1"/>
    <dbReference type="InParanoid" id="G3AVL8"/>
    <dbReference type="OMA" id="WHIEGAM"/>
    <dbReference type="OrthoDB" id="415532at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025340">
        <property type="entry name" value="DUF4246"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049192">
        <property type="entry name" value="DUF4246_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049207">
        <property type="entry name" value="DUF4246_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33119:SF1">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33119">
        <property type="entry name" value="IFI3P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14033">
        <property type="entry name" value="DUF4246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21666">
        <property type="entry name" value="DUF4246_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4246" evidence="3">
        <location>
            <begin position="16"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4246" evidence="2">
        <location>
            <begin position="82"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="277"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW29967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="562" mass="65996" checksum="E56CDA4B4E7F8B10" modified="2011-11-16" version="1">MSGQKRSHGATMLVGSPFPHPWCTDFGLFSARSLDDWLIMEISQSLRSKNEWQRKYKDETIASKWKQEIRDQCKNKTNYMEEVIDYVFKELEWYEELESTLMESSGFEIGCNEYMAISNSVITWEVKEDLKARVLPLVESFGEALDYHPGSDNKVIDLVHPSLFPLQYDITPVFGKDNKLEIVKYTEEIQHAKINVFNHAVSKKFQWIPALLKLQDNGKFTFSSYINNLHPVKHKELYSSIEDVFNAIIPGLNYTLSRYASKEHLRVRVPPGEDAYTEELDEEKDAIYDRIDEMDQDKEEEIEALYDQISDLEKDKSRFVLKTIRPNWEENHPVLDQIIDVRTFGSLKVIVKLANIELTPENPSYNGGSWHVEGTINEDIVATVLYYYDMDNISESKLSFRTDYSQPNYAQHDAFYLEHFYGLKDSDLMMKNIGSIEAKENKVVIFPNMYQHHVDSFELKDKTKSGHRKILCFFITDPYNSNVVSSDRIPPQQKEWWDDEQLSYLYPDNTKQEILQLKSESNQEWPLTLNYARPVREELMKERSIPQDVENDFDRHFCFCEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVS2</accession>
    <name>G3AVS2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_144601</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007378003.1">
        <property type="nucleotide sequence ID" value="XM_007377941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVS2"/>
    <dbReference type="GeneID" id="18870647"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144601"/>
    <dbReference type="eggNOG" id="ENOG502RQDB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_205481_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVS2"/>
    <dbReference type="OrthoDB" id="2163284at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="67" mass="7634" checksum="F950299B22684A2C" modified="2011-11-16" version="1">MTRSKSFIAQASAHANLSKQEKIDLILQLRAKIDGLISNIRDSKTLCDKYDSEIQYFQEYIGGLVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AED3</accession>
    <name>G3AED3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_147282</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373133.1">
        <property type="nucleotide sequence ID" value="XM_007373071.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AED3">
        <property type="interactions" value="237"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AED3"/>
    <dbReference type="GeneID" id="18870746"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_147282"/>
    <dbReference type="eggNOG" id="KOG1910">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000741_0_0_1"/>
    <dbReference type="InParanoid" id="G3AED3"/>
    <dbReference type="OMA" id="IQLKWNN"/>
    <dbReference type="OrthoDB" id="1562405at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019441">
        <property type="entry name" value="FMP27/BLTP2/Hobbit_GFWDK_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019415">
        <property type="entry name" value="FMP27_SW_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019449">
        <property type="entry name" value="FMP27_WPPW_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045167">
        <property type="entry name" value="Hobbit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678">
        <property type="entry name" value="ANTIGEN MLAA-22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678:SF6">
        <property type="entry name" value="BRIDGE-LIKE LIPID TRANSFER PROTEIN FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10344">
        <property type="entry name" value="Hobbit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01214">
        <property type="entry name" value="Fmp27_GFWDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01215">
        <property type="entry name" value="Fmp27_SW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01216">
        <property type="entry name" value="Fmp27_WPPW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="5"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27 SW motif-containing RBG unit" evidence="5">
        <location>
            <begin position="1056"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27/BLTP2/Hobbit GFWDK motif-containing RBG unit" evidence="4">
        <location>
            <begin position="1173"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27 WPPW motif-containing RBG unit" evidence="6">
        <location>
            <begin position="1569"/>
            <end position="2053"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="672"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1019"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2389"/>
            <end position="2446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2573"/>
            <end position="2599"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1745"/>
            <end position="1801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="687"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2391"/>
            <end position="2408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2409"/>
            <end position="2428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="2436"/>
            <end position="2446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2573"/>
            <end position="2588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="2629" mass="304273" checksum="F8B1421CAF933326" modified="2011-11-16" version="1">MTYIFWLLIFIQILPYVIVLILLNCSKYVTVKYIAWLSLRQVTIRLKTTKIHIRKISLRFNFFRSNETSGLKLINLEIVDVEVTTLESGQQAGHDTPAKPAKTSKGGSLSRAVTFHMSKKIYSFIESRICNRINIHVFRLAVKGKTSIFCDYTRLQVTINEQHDFRFTITMLSGLIAKDKKQNTHLLRNLEFYIDCNSVTTCNDQNRVSLQLINFKFALSIGRLNLPLEYLELSKPRKEVSKGPKELPDLLKYVGLLQEFLDFFSSAEIRLEDFTLSHKQLSINLTSIISLTKIDKDTYSNFKLSWYLTSFKLYHKETQCVEIPSGTLNHEFDPLEAVRVMNALLHRQESQDTINFNISLILTSPTIDVYYDQQEILFAIQRNKMMKRMQQSTVKNDMANEILMTIVQMIRSISGTIDIVDTHLNIHLPARDSKHEDFNRNSKSNLIFKCSLSDLMGKIFTRRRRNKTTIDSLIKLKEFRSEVEGNRISISKITSLITYNVLSNTVAIRVASKIVRMKSVNDMFFHIVRQYRNQQRIFNNEKYKEWIKSADYEAFIENNHTFQPVKSDYLQLFEIIPSIVTSINVKLGTILLDIICKEGLPSHMIYDENLQKEVDLADFRRGISLRIKDTRFIYKSKKEHVELTSNCVELYTLSEYDSEYIEDFDQVTEYSETDSEYGDLSSIETSNDIEDEEDEREDECTKRIKRVLSISGIDLSNKHKSDDINKLILTVPSVDARIDMFFVWCIFYAKTMVERFAPTVESNCSKDMLRQIAPRKKIKLDVNLDSVALVTRLPRKVDILIEVDTAQFRNVSSLKSVDIGYARLYVIQPATKLWARLVVITQTKLNIDLRGKTDITPASIRFNVPNRFLMYTVIDNLITFFKAIKQLKHNFWGVSLEFDKFERCYPSQKLHPMKFPCIRIRTNILGLALENDAFENELAMIYELGIVEHKERLRKLKIFEKKAEEILSQAQEEDIEHKIELSEAVPFGPRLNEHRPHSPNPFKAAECKISQSTARFKERVSKLSRKPSKPAEQTSSDPEEPPLSYTVADALDQIEAARSRLYENMSTAWVNRYRFFRKEKTESWRTRTEAVWGKDEIRTIIKQKFSILAYAQGAPLMGAVFRDVDLTLDKFNDGDLDEFIYNNARKQPKLKYSILAPFHIDLVAKKLYMFLRDYPLPLISFPPNSDASKPTIHLQGNIVINEKLYEMQEELRYIFVPFSPACIDNGKADNFYSVYVPRTLTPVKMIANIQCDLNTDRACMISFCKSYTPSLSLMFRAFDNFTKPAIDDSPIGWWDKVPLILHGRIQFNVHNELCLHMKSSTDPYALVGDAAGFVFCWKNNVELAIDGNIDSRKLITLSSDDFLFVIPNYGASAKEAWSLFYDDKDDLTNLVDGESKKYLKRVIKLSSKSRVKWTFGMMFERNKHESNNFSDNELRTSEFKPHYEVIVTNPANSYHPDSYKDYRSHYIHMALAVISNSDTGHSYNAAHLTPLTFQYFFRWWDLLLHHTVMPVRNGNLYKGDSLKKKSVSMGKHLFTIKYQLVFNPVIISHMYLHASNDVPDKNNRVQFTGLKSRFTKFEVDMHQRRELITYVNKKLNKRTQVRHLKMNQVEVSTENMDVRLIRATFPDRCVTSKVAQYVKGELMSSASSSSSSDGSLNGRTFSDWMADIDSYNQDYSWLDQEDFIELEVKEPLSPYPKIRIVPFLFTPKFSYFREFTKESEGPFPFGNEPIHDCLMDLEKPAAVQRRILQQRIDRLEDELESQQKLLDRYKLNASPEFEDDIKNLETNILSLNEKIEVVSTARDEFIEEQVDDEDGTSLSKQTSRLSAYSGHLSNDILDGSVSQFHNRFIIHNLQMKWDNQLRDYFLCYLGKVGDRKNNVFFMSKAAVDLVESIINEQQQEEDGLKPESIKQHRSFDPSDFKTSEQVINSFHDEFDQVNEDEEVDNKYLIKLIHPQIQLVSKKNPDACVLITVRDLEIRIIGVNMRDMVNTMDENTENCSMVERRYGVLLKDEQIFVVCKNSIVEKTDPMISNFVSGYSTGADNWPPWFECEVCYQGNNSINSRYMVSDRSTKAVMYKQKNPLFVEASSRSVEKELVVYLGKFVINATSDQYSCIYYVYSDLLFHTRTSKDDVTGRLKKVMALSDSTDFHGLDIRVKTLQDQIREYSELLLSFDVKRAQHHDFEKESLAVLQVELVKLKFELMYVMKGISLRFKSKQISSSYWNVLADQIIWHFLDDNREPFVDFALGNTRFTRTESADGTNVNNLQVSFLQGFNLQKDALYPQLLTPYESAPDGKRLLNMSWTLMEPIGGIRIVKDAKVEIQPLQLELEFSTAKKLVSYLFPKDDSVDMDDSQEHDDFDDIFEQEEEGYASETSSNKSNSSKNPIKRLIQKSKPSSSNWSANASTSVSDNMSPRNSSEMSSITSFSNDEFSEGTKTKTKKSTRLKKKRIEDDEDNLALVTARSAKYKSIIHVEIVKFILVVSFRGPKNNHLLDVNQLQLNVPNLTYNHKIWSGEDLMNRIKKDIIKMILQHTGKILGNKFKIPKKRSKLSEPLKQISNYGKLLTLDDLQQQHQGRDSMDSLDSPRSESPRVTPKPHVIKKMDASDATFSTYFRDDSSDISGDDEMVVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEG1</accession>
    <name>G3AEG1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_58953</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373161.1">
        <property type="nucleotide sequence ID" value="XM_007373099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEG1"/>
    <dbReference type="STRING" id="619300.G3AEG1"/>
    <dbReference type="GeneID" id="18872693"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58953"/>
    <dbReference type="eggNOG" id="KOG1318">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046871_2_0_1"/>
    <dbReference type="InParanoid" id="G3AEG1"/>
    <dbReference type="OMA" id="HRVRREN"/>
    <dbReference type="OrthoDB" id="71302at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11398">
        <property type="entry name" value="bHLHzip_scCBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047206">
        <property type="entry name" value="bHLHzip_scCBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47787">
        <property type="entry name" value="CENTROMERE-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47787:SF1">
        <property type="entry name" value="CENTROMERE-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="4">
        <location>
            <begin position="129"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="49"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="49"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="104"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW35749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="209" mass="24049" checksum="31A1D73D42EDDE06" modified="2011-11-16" version="1" fragment="single">MSKREGDERVRSEKKVKLSKKEEIAIDSELLGQQSSYVNNNKEGEDASIAEAAVAAVNDSDQKQAQQQHQQQQQQQQQQAQQSQQQSLSYHQAYQQQQEAQGQNPFRPTTLPPTSANKPTHGSDEWHRQRRENHKEVERRRRESINVGIKELAALIPTNDTNKAQILQRAVEYIKRLKENENNNIEKWTLEKLLTEQAVSELSASNEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEH2</accession>
    <name>G3AEH2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RNA polymerase II core subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_69148</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372146.1">
        <property type="nucleotide sequence ID" value="XM_007372084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEH2"/>
    <dbReference type="FunCoup" id="G3AEH2">
        <property type="interactions" value="559"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEH2"/>
    <dbReference type="GeneID" id="18875258"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_69148"/>
    <dbReference type="eggNOG" id="KOG4392">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090381_2_1_1"/>
    <dbReference type="InParanoid" id="G3AEH2"/>
    <dbReference type="OMA" id="MNAPSRY"/>
    <dbReference type="OrthoDB" id="10248581at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06926">
        <property type="entry name" value="RNAP_II_RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.10:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037685">
        <property type="entry name" value="RBP11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008193">
        <property type="entry name" value="RNA_pol_Rpb11_13-16kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I,II,III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946:SF16">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01154">
        <property type="entry name" value="RNA_POL_L_13KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RBP11-like dimerisation" evidence="6">
        <location>
            <begin position="31"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="122" mass="13955" checksum="7580DCB43085E848" modified="2011-11-16" version="1">MNAPDRFELFILPDGVDKIKITPDTKVPNAAIIKIEREDHTLANLLRNQLLKDEKVIFAAYKVEHPLFANFVLRIQTEDDYSPREALKNACSGLISELDAIKSRFNAEWALKALLNNNEDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEI5</accession>
    <name>G3AEI5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_130993</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372159.1">
        <property type="nucleotide sequence ID" value="XM_007372097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEI5"/>
    <dbReference type="GeneID" id="18869545"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_130993"/>
    <dbReference type="eggNOG" id="ENOG502RQ2J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075099_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEI5"/>
    <dbReference type="OMA" id="ESIMTNY"/>
    <dbReference type="OrthoDB" id="4082216at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040134">
        <property type="entry name" value="PSMD12/CSN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12/COP9 SIGNALOSOME COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="1">
        <location>
            <begin position="225"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="325" mass="37620" checksum="29C8FB9B9E35CB99" modified="2011-11-16" version="1">MTTTNLLIDIINDSSIIDNIKVKQLSVQISQFNDVDIVSLNPSELPVDTSYKYIILLLKTEKILAQDPYNPILKQLVVDVNSIPPVAPNINENDFNSWFIKVKHNDLVTDIAYLITDLKYDNFIDLINKKLLNVKSVPTSNPYYSQLTVLIKLKILHLYLLSNYNFRNLNIAHYLQENLIAEEVSGDIWQLFENFKTNALISHDLFNLIVSANFNDNYQKIIEKMDKTKLYMNILENNIIRLSKYYTSIKISRIGEMFQFQEKGINVDLENLLFDMIIRKKLNAGSKIDQLENILQFEESAENSVQLNDHIKQVGTLISDICIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEK4</accession>
    <name>G3AEK4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">cystathionine gamma-synthase</fullName>
            <ecNumber evidence="10">2.5.1.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">O-succinylhomoserine (thiol)-lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_57835</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of L-cystathionine from O-succinyl-L-homoserine (OSHS) and L-cysteine, via a gamma-replacement reaction. In the absence of thiol, catalyzes gamma-elimination to form 2-oxobutanoate, succinate and ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-succinyl-L-homoserine + L-cysteine = L,L-cystathionine + succinate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20397"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57661"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="EC" id="2.5.1.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-cystathionine from O-succinyl-L-homoserine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the trans-sulfuration enzymes family. MET7 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.48" evidence="10"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372179.1">
        <property type="nucleotide sequence ID" value="XM_007372117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEK4"/>
    <dbReference type="FunCoup" id="G3AEK4">
        <property type="interactions" value="191"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEK4"/>
    <dbReference type="GeneID" id="18872213"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57835"/>
    <dbReference type="eggNOG" id="KOG0053">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011302_1_0_1"/>
    <dbReference type="InParanoid" id="G3AEK4"/>
    <dbReference type="OMA" id="KVAKRCR"/>
    <dbReference type="OrthoDB" id="10047078at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003962">
        <property type="term" value="F:cystathionine gamma-synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000111">
        <property type="entry name" value="Cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000063">
        <property type="entry name" value="Probable cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051750">
        <property type="entry name" value="Trans-sulfuration_enzymes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42699">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42699:SF1">
        <property type="entry name" value="CYSTATHIONINE GAMMA-SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="4 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="169"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="194"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00066530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="591" mass="66295" checksum="D4319533DA44E1F4" modified="2011-11-16" version="1">MQLQTSPSQEIGMPIPNIPHAVSVTLPTWEATVGYEEGEDWVVSKMRSGYPRFFIHPIIQDLSKRVEHKYGRDGERCMIFPSYKVAKRCREFIQSKAKTNASRRVLQLSTPAPASEDDKSTVIAATIGVVFFPASEYPLAKNYWQHSGEGISSRMGEYVLKELFEKEPVSNGRTGNMKSELQSQKIQSRSPSISAARGGSQSNSSNNGSAEVDKEFSTFIEQKYGRVLDLKFTQGAKMALRRRICGKVDKSKNQIEEMEKARRGKHLGETDVYLFPSGMASIFFAHFSLLQTSKGEPKKSVCFGFPYVDTLNILKKFGPGVYFYGLGDDDSLDELEQGLESGAIDIMALFCECPSNPLLKTPNLARIRALADKYNFAVVIDETVGNFLNISVLPYADIVCSSLTKVFSGDSNVLAGSLILNPLSSYYPTLKKFFNEEYEDLFWAEDALWLERNSRDFAERVHKIDNTAEQVVDLLNNNDLISKVYYPSLSASRKYYDVIKNVNGGYGGLISFLFKDPEHAVKFFNVVNLHKGPSLGTNFTLACPYAILAHYQELEEIAQWEVDRNLIRISIGLEDPVELLKVLQSSLQECV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEN2</accession>
    <name>G3AEN2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HotDog ACOT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_133086</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl coenzyme A hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373070.1">
        <property type="nucleotide sequence ID" value="XM_007373008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEN2"/>
    <dbReference type="STRING" id="619300.G3AEN2"/>
    <dbReference type="GeneID" id="18869718"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133086"/>
    <dbReference type="eggNOG" id="KOG2763">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032862_1_0_1"/>
    <dbReference type="InParanoid" id="G3AEN2"/>
    <dbReference type="OMA" id="RAESHNA"/>
    <dbReference type="OrthoDB" id="331699at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03442">
        <property type="entry name" value="BFIT_BACH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000032">
        <property type="entry name" value="Acyl-CoA thioester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000038">
        <property type="entry name" value="Acyl-CoA thioester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655:SF0">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="5">
        <location>
            <begin position="104"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="HotDog ACOT-type" evidence="5">
        <location>
            <begin position="310"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="501" mass="56269" checksum="284EDE691B7A49F9" modified="2011-11-16" version="1">MLKTLTSRSITYKGVPAIAKRILPFRSLTHNFHQPDVAGSAEPAQAADAAATILADLRDKQTNPNKATWIAALKEREKQLAEGKVIDSFAYVDPKTTVIGEKTRADSFSYLTLPFKQDEWLCDAYINAFGRLRAGQLFQDLDALAGRIAYRHCAPAEPVNVTASVDRIYMVKKVDEIVNYNFVLAGFVSWTGRSSMEITVRGYAFEDELPETINHDTLPMDNVFLTANFTFVARNPITHKSFAINRLLPVTEQDWIDYRRAESHNAKKKLSAKNPTMIAPTPEESNVIYHMWKSSANLPEPLQTGISYMKDTRMSSTLFMQPQYRNRHSYMIFGGYLLRQTFELAYCTAASFSSAGPRFVSLDSTTFKAPVPVGSVCSMVASVSYTEHLHDDTTSHEQDSPFDFKLPSTNKISSNPDAFLSEPGTLIQVKVDTFIQHLESQNKKEAGTFIYSFFVPHEDKQGFCSVIPQTYSEMMAYIGGRRRAQDTAGYVETLPKPNKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEQ0</accession>
    <name>G3AEQ0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">mRNA decay factor PAT1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_58881</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PAT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373087.1">
        <property type="nucleotide sequence ID" value="XM_007373025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEQ0"/>
    <dbReference type="FunCoup" id="G3AEQ0">
        <property type="interactions" value="233"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEQ0"/>
    <dbReference type="GeneID" id="18872661"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58881"/>
    <dbReference type="eggNOG" id="KOG4592">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012622_1_0_1"/>
    <dbReference type="InParanoid" id="G3AEQ0"/>
    <dbReference type="OMA" id="YLEHSGH"/>
    <dbReference type="OrthoDB" id="74835at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045947">
        <property type="term" value="P:negative regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039900">
        <property type="entry name" value="Pat1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019167">
        <property type="entry name" value="PAT1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21551:SF0">
        <property type="entry name" value="PROTEIN ASSOCIATED WITH TOPO II RELATED-1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21551">
        <property type="entry name" value="TOPOISOMERASE II-ASSOCIATED PROTEIN PAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09770">
        <property type="entry name" value="PAT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="mRNA decay factor PAT1" evidence="8">
        <location>
            <begin position="99"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="mRNA decay factor PAT1" evidence="8">
        <location>
            <begin position="514"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="188"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="143"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="193"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="223"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="282"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="305"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="320"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="788" mass="87607" checksum="997CDB04BF016431" modified="2011-11-16" version="1">MSFFGFDPTAPPAGRNNNKKEVYEFQETYEGLDEADDAFNDETFGTSTGEIRKDFDFGAGAGAGAGASAPPAAAPATASTGSVSQGVSYAQAAAPTDDEFMQDLWGTNTPIKHEQPIASGERKILSLEEIEAQLTAIDPSAQPPQQQQQQPQGQFMQPYMMPPPQFGYMMPPPPGAYMPPPPPPQAYGQMPGWMPPPPMGVPPQPPVGQPVPQQSQPQPIPNTAGSVQPAEESQVQEGAPETAVDNSVVSSSPPKKVDLSHFPVLGSKEAHDAHHQPHRQHQQQQQQQQQQQHQQQHQHNHIAPLQSQVHPPQHTLTPEEQERAARRQEKVSRIMKYSGIMNPKDKDFVTRFQLSQIVTEDPYNEDFYAQVYKVIHPKNDNVQPMGSTNNSIAQAYLEQSGHRLGGRYKRADVALQRMQQQVQKAVTVAKERPKLTQYAKEGALGKISFGTGKKPRQQLEIISKAAERERAGEVKQDKEITSYSKKDILNILEGIITELMNVESETRALHDIDTSKLWDSLKVLEHASCSGDPEPEVNPFIQCLNYNKMLKILPRLFKFLNREQILTITTLIMSNLENLAVIKHGSYTTYANKRVPENIAHLVDSYSMTFSKVLMNAVLDFKFNEIIGLLVILIEHNNVSFVSTTKIGLSILTTLLSRAELIIGEGTISATDLSEWSSCYDELFTSLESRIAAIFPPHPEDVDDGSSRENYVWQFLATLSLGGKLSHQRIIVDEVRDEIFGVMARAKDIDPADVANLYKKQNLLNNLNMYLVVMGLVADENEIKELQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF49</accession>
    <name>G3AF49_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Autophagy protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_132827</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in cytoplasm to vacuole transport (Cvt) and autophagic vesicle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Conjugated with ATG12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Preautophagosomal structure membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ATG5 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372250.1">
        <property type="nucleotide sequence ID" value="XM_007372188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF49"/>
    <dbReference type="FunCoup" id="G3AF49">
        <property type="interactions" value="345"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF49"/>
    <dbReference type="GeneID" id="18869696"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132827"/>
    <dbReference type="eggNOG" id="KOG2976">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051894_2_0_1"/>
    <dbReference type="InParanoid" id="G3AF49"/>
    <dbReference type="OMA" id="SIQKAVW"/>
    <dbReference type="OrthoDB" id="272162at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034274">
        <property type="term" value="C:Atg12-Atg5-Atg16 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044233">
        <property type="term" value="C:mitochondria-associated endoplasmic reticulum membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061908">
        <property type="term" value="C:phagophore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019776">
        <property type="term" value="F:Atg8-family ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.190">
        <property type="entry name" value="Autophagy protein Apg5, helix rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007239">
        <property type="entry name" value="Atg5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048940">
        <property type="entry name" value="ATG5_HBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042526">
        <property type="entry name" value="Atg5_HR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048939">
        <property type="entry name" value="ATG5_UblA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042527">
        <property type="entry name" value="Atg5_UblA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048318">
        <property type="entry name" value="ATG5_UblB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13040">
        <property type="entry name" value="AUTOPHAGY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13040:SF2">
        <property type="entry name" value="AUTOPHAGY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20637">
        <property type="entry name" value="ATG5_HBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20638">
        <property type="entry name" value="ATG5_UblA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04106">
        <property type="entry name" value="ATG5_UblB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <keyword evidence="5 7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Autophagy protein ATG5 UblA" evidence="10">
        <location>
            <begin position="18"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy protein ATG5 alpha-helical bundle region" evidence="9">
        <location>
            <begin position="143"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy protein ATG5 UblB" evidence="8">
        <location>
            <begin position="205"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="292" mass="33731" checksum="39AFCBB7B03104C8" modified="2011-11-16" version="1">MNFTGASSSATTEIKRKLWNGCINIKVVLPHDSEDIEYLINIPRNSYLPIHFQQLISYFQNYVSYEIYKQALWFEYEGVPIAWNLPVGVLYDYLYLPSVIQERGGGGLDQNCWTIQLNLRGKYPAEYIIPFVYTNPDNTINYEQSLNEIIVNQLKQSCFVINGSAKPIMNLSESNSKLLWNSIVTRNLGQFTSLNKKIIPKQFQRIPVKIYIPGSTTVIQAPIHAYEDDKPVTLSRVLQRELPNLFTNDVKLARTYIHGIDISSIMDMPLHDIWTIFRHLDNFLYIIIIAMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFE7</accession>
    <name>G3AFE7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_58065</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372348.1">
        <property type="nucleotide sequence ID" value="XM_007372286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFE7"/>
    <dbReference type="FunCoup" id="G3AFE7">
        <property type="interactions" value="357"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFE7"/>
    <dbReference type="GeneID" id="18872303"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58065"/>
    <dbReference type="eggNOG" id="KOG1838">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032487_1_0_1"/>
    <dbReference type="InParanoid" id="G3AFE7"/>
    <dbReference type="OMA" id="GCCRTKI"/>
    <dbReference type="OrthoDB" id="5954035at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008126">
        <property type="term" value="F:acetylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051792">
        <property type="term" value="P:medium-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051793">
        <property type="term" value="P:medium-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00761">
        <property type="entry name" value="Glyco_tranf_GTA_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012020">
        <property type="entry name" value="ABHD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF44">
        <property type="entry name" value="MEDIUM-CHAIN FATTY ACID ETHYL ESTER SYNTHASE_ESTERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005211">
        <property type="entry name" value="Ab_hydro_YheT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="150"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="373"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR005211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="429" mass="48520" checksum="993A9D3964939643" modified="2011-11-16" version="1">MGILNWGFRANIKVHHVDDDNSIAFPTRNGSGTIKFTEFIRENLSLIDPSKRLWLNPLLFTGSLQTLYYASADSSSKFQIYYGRELFTYSDGGICSLDWVIPKPEDKDAFKEQAKESVPEGFPRLHPRTRYLTDDEVDARRAGDVASDVPLVVIFHGLGGGSHESLIRNFAESIQKNTNDHWDIVVVNNRGCCRTKITSAKLFNAGSTDDLREVLVDFKKRYPKRPIYAAGFSFGAAMLANFLGEEQEHLEEKTEDLVKAACVVGCPWDFVDSAYHINESWTGKYLLNPSLAQFLNKIVKNNAEDFNKFDPTFLTEEAIARAAKAKTTWEFDDAITSKTTPYKNAFEYYRELSPQRRIQKINTPLLIINSTDDPAVGTRLPILDAKTNPYLCLVETDLGGHLGYVKTSGEFWCVEVIEQFFQKFAEVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFK8</accession>
    <name>G3AFK8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome biogenesis protein ERB1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Eukaryotic ribosome biogenesis protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ERB1</name>
        <name evidence="10" type="ORF">SPAPADRAFT_132853</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat BOP1/ERB1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372409.1">
        <property type="nucleotide sequence ID" value="XM_007372347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFK8"/>
    <dbReference type="FunCoup" id="G3AFK8">
        <property type="interactions" value="1070"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFK8"/>
    <dbReference type="GeneID" id="18869699"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132853"/>
    <dbReference type="eggNOG" id="KOG0650">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011390_0_1_1"/>
    <dbReference type="InParanoid" id="G3AFK8"/>
    <dbReference type="OMA" id="MRPAKGE"/>
    <dbReference type="OrthoDB" id="5571054at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000061">
        <property type="entry name" value="Ribosome biogenesis protein BOP1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03027">
        <property type="entry name" value="BOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028598">
        <property type="entry name" value="BOP1/Erb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012953">
        <property type="entry name" value="BOP1_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605:SF0">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1 BLOCK OF PROLIFERATION 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08145">
        <property type="entry name" value="BOP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01035">
        <property type="entry name" value="BOP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BOP1 N-terminal" evidence="9">
        <location>
            <begin position="166"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="431"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="779"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="545"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="27"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="91"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="549"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="805" mass="92035" checksum="6A9333AAA52001B1" modified="2011-11-16" version="1">MAKSKAAPAKETVTKKESVTKKRKPEEVEEEPESDEEFQVDGLIDGDDDSDESEEDEEDEEDEEDEEADSEEEEDNGDFDSEEELNKLLGEEEDPSDYSSDEFSDEPKEEISGLPLKTLSINENVKSKYSDGTTRIIKPEINPVYDSDDSDAENFNTIGNIPLSAYEEMPHIGYDINGKRIMRPAKGSALDQLLESIDLPEGWTGLLDQNTGTSLKLTDEELELIRKIQQQESTDENINPYEPLIDWFTKDQEIMPLTAVPEPKRRFVPSKHEAKRVMKIVRAIREGRIIPPDKVKKELEEEELNFDLWKDEQEIPDHIMNLRAPKLPPPTNEESYNPPAEYLMTEEEKEKWLAEDPQDRERNFIPQKYSSLRQVPGYQDSVRERFERSLDLYLAPRVRHNKLNIDPDSLIPELPSPKDLRPFPIKCSTVYQGHTEKIRTISIDPQGLWLATGSDDGSVRIWEILTGRQVYKVQLIDKEINSDDHIESLEWNPDSTTGILAVCVGEHVYLLVPPIFGFDIENIGKLRIEAGWGYDTFGNKKQELKVSGEDDEEVDDEEEETTTEKKKDVSKWFPPNAEQATMGISAIIQCRKTVKKISWHRKGDYFVTVSPESSNTAVLIHQLSKHLSQSPFKKSKGIIMDAKFHPFKPQLFVASQRQVKIYDLAQQVLIKKLMPGVRMLSSIDIHPRGDNLLACSYDKRVLWHDLDLSSTPYKTLRYHNKAVRSIKFHKGNLPLFASASDDGNVHVFHGMVYDDLMTNPLLVPLKKLTGHKIINQIGILDLVWHPKEAWLFSAGADGTARLWTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFP2</accession>
    <name>G3AFP2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rho1 guanine nucleotide exchange factor TUS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_53388</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372443.1">
        <property type="nucleotide sequence ID" value="XM_007372381.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AFP2">
        <property type="interactions" value="121"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFP2"/>
    <dbReference type="GeneID" id="18871781"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53388"/>
    <dbReference type="eggNOG" id="KOG4305">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004667_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFP2"/>
    <dbReference type="OMA" id="HEIITWE"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057283">
        <property type="entry name" value="RGF3_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052233">
        <property type="entry name" value="Rho-type_GEFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572">
        <property type="entry name" value="RHO1 GDP-GTP EXCHANGE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572:SF1">
        <property type="entry name" value="RHO1 GUANINE NUCLEOTIDE EXCHANGE FACTOR TUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23582">
        <property type="entry name" value="WHD_RGF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="4">
        <location>
            <begin position="529"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="759"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="5">
        <location>
            <begin position="977"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="828"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="848"/>
            <end position="857"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1313" mass="150969" checksum="C3101F61069F6AE1" modified="2011-11-16" version="1">MSQHSPSSGKRSLRRKPPAEMEPLNQPVPANNSTFPYTTRSTQQPFQPEVDPHFKRRPSQDYSPSNQQRYEFLNYDTSIAPDYSHADQSYEDTFASLDSPKPKGPRQIWEYNEHEYREASPTPLGNNRDLDHRHATRDRSPIHIDRTKPKERAFQEMSRSNSIPSVATPYPTKPVEEEIFDEEFAFTVSPPDTKRTNETDRIPSLVQRRSMPALPVSTLAHSTNYNNLASIGHDARNLNAPEFDENEFIESQGSSSRWNSIRSHDSYYAVTDNPFFDEEELEMRTESPTSDYFDYSILPDIPTGKDASFLEPTIPPHTHIPIPGIPSIPRATPYKKLPVIPLDLPELPFSSSLLLSQHFQICENIWSLSSIFQWCLELRSWSRDQTISKRELKKALIRLFAFHRSDFALDVITRNANIVLTSLVDSGAIVLVDGKGSEEEKKGITMASDVYVNGVLPDFTQCYSVFPHAEDDETSYQCYSSSCYSNEVRKYEARRRTTNIKDIVLENDWATHWHLSAEDLRGIEQGLIKRQSYVFDLLRYEQTFIQRAKCFIEIVVPEFVPHMRELFKPEITKKFEQDVVEPGRKILEIHQTQLFEPLLRILIAEGKFIRDLVSISNIYNDWAIEVRPFLMQYMSCMPLIEELLSTPSLKTWVDNHIGNLKRVKELKVNVAILFISTFNSRYQSLPLQLLDIRKKYDESDEEYHALTKASETIKKLGSKINESKRMADNVHTIGMIKNQLVWKNNLQPTNLNLGSTNRKFICRGDMTRKGEMKITTWINHIILLDNYMIITDRVKNSKARGYSYRIIEDPIPVEFLIVEEKAMTGVTTNTLPLPRNGTNQLPTPPPTTTTTSNTLDSLGDDDDSATFALKIRFAGRQKHTYTFSCKSERERGEWIRFLSETKSALGKRLKPSEPFDMKLVSNTCFGYEFPNRITKLQICAPYDPIYEISQDSLRKLTQLGYKSDLYSFPNSRNHVVFSRVNYLCSFTYKGGRYHFIGLATGLYCCEAKNRWKKILNGADFTKIHVDVVAGIVIVLGDKHLKYFLLSQLLLVYTEKKSEVTSIPVSKEPVLFFSVGRHKNITMLFYAKRKSNGTTHFKVLIPETDNHGVFSRFKEERKFYIQAECYGISIFNSSFAVHTNKGFEILELDKLIPRSVPDLPLPDVENKKKIDQFQRRTSNSSNVAVEIIRKLVSSSNMKPLGMFKLNNNTEFLLVYNEFAIFINKHGKLSRLSVLRFEFKARSIQFANNHLCIICEEVIEIWSISDFVKGTNKLMQVITGKDIRSISDDQIAIAMANPMVLGLQLVFQLISKAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFR6</accession>
    <name>G3AFR6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF2415 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_58189</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372467.1">
        <property type="nucleotide sequence ID" value="XM_007372405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFR6"/>
    <dbReference type="GeneID" id="18872363"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58189"/>
    <dbReference type="HOGENOM" id="CLU_1126861_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFR6"/>
    <dbReference type="OrthoDB" id="1068471at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051980">
        <property type="entry name" value="WD_repeat_MORG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22842:SF3">
        <property type="entry name" value="WD REPEAT DOMAIN-CONTAINING PROTEIN 83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22842">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="247" mass="27495" checksum="CACEE76794A85036" modified="2011-11-16" version="1" fragment="single">MQSANQECVYWSYQVQKDAATTFSRVEGSNYVLTASKNKIQLKNLGDRASKQTFLTQIKYNDILSLDSCPATLKVLSSSSDIAFVNDMITGDEITRLSNDNTNRGIILDCIFIGNDVVATCGNDSTLTLFDVRENPVKVKPWSFTCIPNDIFTSIGYSYPCLAVGSVSGTIYMVDVRSQKSISPWISPSSMLSVDQHKNLSISFDSTGVIRLYNVRNNILLANKKPVGQKVRTRINCKYVPERNFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG08</accession>
    <name>G3AG08_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_132293</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372559.1">
        <property type="nucleotide sequence ID" value="XM_007372497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG08"/>
    <dbReference type="STRING" id="619300.G3AG08"/>
    <dbReference type="GeneID" id="18869651"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132293"/>
    <dbReference type="eggNOG" id="ENOG502S2DH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1120503_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG08"/>
    <dbReference type="OMA" id="MEVEEFC"/>
    <dbReference type="OrthoDB" id="5069333at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF49">
        <property type="entry name" value="LYSINE BIOSYNTHESIS REGULATORY PROTEIN LYS14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="232" mass="26999" checksum="4F536D58A91CC4A6" modified="2011-11-16" version="1">MGCDRRLLNIISDITDLSFERFRNSISETNYAILCNDMKKKLDEMNINMMESVLASSKSDAELMVQEFGMEVEEFCFLLSCEIKRLATILYLEACLLNKTPEDEQIDQLVHQIFRLLEFIVIKNNYKWYSTLIWSVFMAASEISSLSPDCEDLRYLTLQIFDKLEDNTLGNVGKTRQIVLSIWKRRDLDNCDENSFGLMADNSKKNKKKGLMGWVNDWEKYVVDEDYAIALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGI3</accession>
    <name>G3AGI3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58548</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372734.1">
        <property type="nucleotide sequence ID" value="XM_007372672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGI3"/>
    <dbReference type="FunCoup" id="G3AGI3">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGI3"/>
    <dbReference type="GeneID" id="18872501"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58548"/>
    <dbReference type="eggNOG" id="ENOG502RQ2F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119130_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGI3"/>
    <dbReference type="OMA" id="TVTEPCI"/>
    <dbReference type="OrthoDB" id="4026603at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442043" evidence="1">
        <location>
            <begin position="19"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="139" mass="14250" checksum="59E538FA6BF11AB0" modified="2011-11-16" version="1">MQYSSVVIGSALAASVLAAANNTLTITTQVTLTDYTTYCPEPTVLTITVCEEEEICSTKSIAVPVPGTITVTEPCIVETVYTTVCETTPSYGNSTSVLPTKAPATTVPPLTVTTYEGGAVKNMYGAFAGVAALAAAALM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGJ0</accession>
    <name>G3AGJ0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribulokinase/uridine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_131669</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372741.1">
        <property type="nucleotide sequence ID" value="XM_007372679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGJ0"/>
    <dbReference type="FunCoup" id="G3AGJ0">
        <property type="interactions" value="240"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGJ0"/>
    <dbReference type="GeneID" id="18869599"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131669"/>
    <dbReference type="eggNOG" id="KOG4203">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021278_1_0_1"/>
    <dbReference type="InParanoid" id="G3AGJ0"/>
    <dbReference type="OMA" id="EPICINI"/>
    <dbReference type="OrthoDB" id="738517at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="1">
        <location>
            <begin position="16"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="260" mass="29766" checksum="BA8910426BC58043" modified="2011-11-16" version="1">MTTSTTTSNRSIIVLLGGGHAAGKKTTANSLKQELSKIPSTNVDIEIIDMNQFKEGCNASSIDTTEYYNQGKSAAITVSKEFHHDKYPSLKPSRFQFDKLKEVLRSKLYIPVDQPQKVLIVHGLYALYDKEIRDLSNIKVFIDSDSDTRLIRWIRRDVLERKTNTLDSVINAYLLGARREMNDFIFPTKEFADVIMPKGANEPNDAVKLVIDGIILYLTDKRPETMYLPTTTSNYLRPTDELSLEKERFDIQKNKFYELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGQ6</accession>
    <name>G3AGQ6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58607</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372801.1">
        <property type="nucleotide sequence ID" value="XM_007372739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGQ6"/>
    <dbReference type="STRING" id="619300.G3AGQ6"/>
    <dbReference type="GeneID" id="18872529"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58607"/>
    <dbReference type="eggNOG" id="ENOG502S7KH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107714_2_0_1"/>
    <dbReference type="InParanoid" id="G3AGQ6"/>
    <dbReference type="OMA" id="SEWAARM"/>
    <dbReference type="OrthoDB" id="3468019at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39401">
        <property type="entry name" value="SNOAL-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39401:SF1">
        <property type="entry name" value="SNOAL-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="16148" checksum="BAAC254B332EC15D" modified="2011-11-16" version="1">MTKYSPTITSSLKKFITDFYLTSDVAPPAALTETRDPYLNFFTEDAPLIMGSTRVKGHNEIIELRKGMWKVVTKRHHVVENVASVEDNNTLLINGYVDYTLINGKSITSEWAARMHLTKDLKMEFYQVYLDPSLTAKALAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH49</accession>
    <name>G3AH49_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_64595</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372891.1">
        <property type="nucleotide sequence ID" value="XM_007372829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH49"/>
    <dbReference type="STRING" id="619300.G3AH49"/>
    <dbReference type="GeneID" id="18874984"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64595"/>
    <dbReference type="eggNOG" id="KOG1502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_9_2_1"/>
    <dbReference type="InParanoid" id="G3AH49"/>
    <dbReference type="OMA" id="SDEPQFN"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="335" mass="36551" checksum="0FFFF1B33F968FA3" modified="2011-11-16" version="1">MTGTTVFVSGANGYIAQHIVKQLLAKGYSVVGSVRSSSKGEELKNLVKSDKFSYEIVPALGDKGAFDNALKKHPEVTVFLHTASPVSFSVNDIEKDLLRPAIDGTVNALQAIKDHGPQIKKVVITSSAVANLGWDKYFDGNKIYTEEDWNPITYQESLENAASGYYGSKKFAELAALDFIEKEKPNFAVSFVNPVYVFGPQAYAIKDKAQLNLSAEVINGVAKLGKNDKIAPFGSHFVDVRDVARAHLVAFENEEAISKRLIVASSGFTLDSIAHIINEHFPNSTVPKGDLSRNPEISKTNIQKVDSSKSNKILGFDLIPLEKSVVDTVQQIYSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH76</accession>
    <name>G3AH76_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptide chain release factor 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_58746</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372919.1">
        <property type="nucleotide sequence ID" value="XM_007372857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH76"/>
    <dbReference type="FunCoup" id="G3AH76">
        <property type="interactions" value="716"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AH76"/>
    <dbReference type="GeneID" id="18872585"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58746"/>
    <dbReference type="eggNOG" id="KOG2726">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036856_0_4_1"/>
    <dbReference type="InParanoid" id="G3AH76"/>
    <dbReference type="OMA" id="DHRVGFK"/>
    <dbReference type="OrthoDB" id="2019491at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070126">
        <property type="term" value="P:mitochondrial translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000004">
        <property type="entry name" value="Peptide chain release factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050057">
        <property type="entry name" value="Prokaryotic/Mito_RF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804">
        <property type="entry name" value="LD18447P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804:SF7">
        <property type="entry name" value="LD18447P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="Release factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prokaryotic-type class I peptide chain release factors" evidence="6">
        <location>
            <begin position="268"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00067174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="405" mass="45433" checksum="6286E7FBF84C1DA4" modified="2011-11-16" version="1">MGSKKILLQWLATSSHHKPHMIRRHLGIQFKRFYSLAPQLHPLLVSRAQTLVQDKKELESKLETYDAELQLKFDKITDIIDRYDRLNTVQSEINELEQIAQDPSDEELANEANKELDKLVPEAESLVENLTNKLLPPIKHAERGCILELRPGVGGSEAGLFTADLMNMYIGFASVNGWKYKVEGKSSGAGVVNEAILSIDTPGTYDILRHESGVHRVQRVPQTETKGRVHTSTAAVVVLPKLSEGNEASLKEDERQFKPGEVRIDTMRAGGKGGQHVNTTDSAVRLVHIPTGVTVIQQDERSQPQNKAKAFAVLRARLAELERVEEVAKQKAMRTDQVTTTDRGDKIRTYNYPQNRITDHRCGFSLHDVPGCMNGTKLGELISQVESWEFKERMQDMIAVEAAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHL7</accession>
    <name>G3AHL7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial glycine transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Solute carrier family 25 member 38 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_70338</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Mitochondrial glycine transporter that imports glycine into the mitochondrial matrix. Plays an important role in providing glycine for the first enzymatic step in heme biosynthesis, the condensation of glycine with succinyl-CoA to produce 5-aminolevulinate (ALA) in the miochondrial matrix.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>glycine(in) = glycine(out)</text>
            <dbReference type="Rhea" id="RHEA:70715"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the mitochondrial carrier (TC 2.A.29) family. SLC25A38 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373765.1">
        <property type="nucleotide sequence ID" value="XM_007373703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHL7"/>
    <dbReference type="FunCoup" id="G3AHL7">
        <property type="interactions" value="108"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHL7"/>
    <dbReference type="GeneID" id="18875319"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70338"/>
    <dbReference type="eggNOG" id="KOG0766">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_0_3_1"/>
    <dbReference type="InParanoid" id="G3AHL7"/>
    <dbReference type="OMA" id="WGIYEEL"/>
    <dbReference type="OrthoDB" id="1924968at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015187">
        <property type="term" value="F:glycine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904983">
        <property type="term" value="P:glycine import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03064">
        <property type="entry name" value="SLC25A38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030847">
        <property type="entry name" value="Hem25/SLC25A38"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46181">
        <property type="entry name" value="MITOCHONDRIAL GLYCINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46181:SF3">
        <property type="entry name" value="MITOCHONDRIAL GLYCINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11 13" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="23"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="128"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="236"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="334" mass="36526" checksum="425A05704D9763C3" modified="2011-11-16" version="1">MQQSPSIISKNDTRNLTTKAKSPEALAHLGAGATAGLVSAITLQPFDLLKTRLQQQHTVKISLASELKNINTVKDLWRGTLPSALRTSMGAGLYFTCLSKMRSNWANFKVKSNPSLNTTSNSSVLPKLTPLENLATGFVARAIAGYILMPITIIKTRFESNIYNYNSMYESIKGIYYNEGTGMVTTVRSGSLRNFFKGSVATLIRDCPYAGLYVLSYESFKNDLFPVLIPVTIDHRASIINSGAAVLAATACTMLTAPFDAIKTRLQLTNESSILKASKILVNEPGGVSNLFRGVSLRLGRKAISSGISWCIYEELIKSDFSQNLFVKKERLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHQ9</accession>
    <name>G3AHQ9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59653</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373807.1">
        <property type="nucleotide sequence ID" value="XM_007373745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHQ9"/>
    <dbReference type="GeneID" id="18873007"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59653"/>
    <dbReference type="eggNOG" id="ENOG502SAYP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077187_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHQ9"/>
    <dbReference type="OMA" id="SDKLWTQ"/>
    <dbReference type="OrthoDB" id="4065597at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17242">
        <property type="entry name" value="DUF5315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="241" mass="27770" checksum="A948E8738F82A776" modified="2011-11-16" version="1">MQPTRDSSKLGVPRTAYPASIHSNNTFRRTTSGGASDRYLSRTNTGTTDPYIAATNAESQSIVTMDKIPSAKPSATYSDLLWTQIDVLDDVRNMASEVRNRGSFFNDKFNEELSKLKQSQNKLLETMSTQHFSDLDASEHQKQLYHLSRMAPVRQSSGNLLDEENIVHENEESKDAFEHKKRQQEKINVFFKEDDLELKNQTIYKKQNFDDVNQYVSEIKQDLQVLGESMKQFDESTREIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHW4</accession>
    <name>G3AHW4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Brl1/Brr6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_59697</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373862.1">
        <property type="nucleotide sequence ID" value="XM_007373800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHW4"/>
    <dbReference type="FunCoup" id="G3AHW4">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="GeneID" id="18873035"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59697"/>
    <dbReference type="eggNOG" id="KOG4503">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031411_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHW4"/>
    <dbReference type="OMA" id="ETHSSMT"/>
    <dbReference type="OrthoDB" id="5961at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040202">
        <property type="entry name" value="Brl1/Brr6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018767">
        <property type="entry name" value="Brl1/Brr6_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136:SF1">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10104">
        <property type="entry name" value="Brr6_like_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01042">
        <property type="entry name" value="Brr6_like_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Brl1/Brr6" evidence="3">
        <location>
            <begin position="278"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="430" mass="48814" checksum="334ECCEAD6355B74" modified="2011-11-16" version="1">MASLFHTQPLHHDIDGSLLQSLSLNDNNNQQQSILTHYDVEDDRMDIDDTEDITMLDTTIQERSEVIPEHVEPKDQHDDEVEEEEEEEEDTSFISSLLSPTMLGAKLAVKPQLLLMAPPGSSPTETKSETVDYEFNTSQMTKVDKPDLFGNSASTSAIGRNISSYSPVSDINTFLAKGDTSGHFNNPSHSTFYNNGMNQSYMMRKTFSSPTPTTVHLHNHYYFNPPKPANHETHQSSLEKLTHQQHQLQLQQAHPHKLALPVPWKSNISPSERIPYMLSSYLQLLLNFLASLYLIHLIRGLTLSIKRDITYRLNQQSTTILTSIETCRRAYIENRCHPDTIVPLLEKKCAMYERCMNQDPFNGGGNLSKITAETVAMVANSLIEPLGIKFFLMMVGFIVVVFGCNFSFGYIRAKTYYGWNNYGEQKHKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHY0</accession>
    <name>G3AHY0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-processing factor 39</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_54448</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
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</entry>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
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        <property type="evidence" value="ECO:0007669"/>
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    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <sequence length="127" mass="13924" checksum="383C5072EDCB085B" modified="2011-11-16" version="1">MSFFSELWESVFTPGTSPALIKATHASFILLLMSLGWLIFLTKSIHFINLFVIAVLLYATVIWFIKELQASKLKTNEELSTEEPKEESKTEKVQEKGSASSSATTAAASAAATSIPATQSTPRRRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI55</accession>
    <name>G3AI55_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase DBP2</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP-dependent RNA helicase dbp2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_59791</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17966">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13 18" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="17">
        <location>
            <begin position="110"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="141"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="331"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="498"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="110"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="503"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="529"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW34369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="545" mass="60326" checksum="BBCE85E0760E1F8B" modified="2011-11-16" version="1">MSYNNNSSEYRNNDHNGYRGGNRDGGYGNRSHGYQGGNRDGGFRGGYQKKFDEPIELVAPEWDLETLPKFEKNFYVEHPNVAARTDREIEEFRRKNEMSVIGNDIPHPITTFEEAGFPDYVLKEVIAQGFPSPTAIQCQGWPMASSGRDMVGIAATGSGKTLSYCLPAIVHINAQPLLSPGDGPIALVLAPTRELACQIQQECSKFGRSSRIRNTCVYGGAPRGPQIRDLARGVEICIATPGRLIDMLESGKTNLKRVTYLVLDEADRMLDMGFEPVIRKIVEQIRPDRQTLMWSATWPKEVQALARDYLNDPIQVTIGSLELAASHTITQLVEVVSEFEKRDRLVKHLETATADKEAKILVFASTKRACDEITSYLRSDGWPALAIHGDKQQSERDWVLREFKTGKSPIMVATDVAARGIDVKGITFVINYDMPGNIEDYVHRIGRTGRGGATGTAVSFFTEDSKKLGGELIKIMREAKQTIPPELQKYDRRSYGAHIRYGPGRGGRGGRGGYGRGGRGGRGGYGGQRNFQSGSNTAPLSNRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIR2</accession>
    <name>G3AIR2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_59906</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374062.1">
        <property type="nucleotide sequence ID" value="XM_007374000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIR2"/>
    <dbReference type="FunCoup" id="G3AIR2">
        <property type="interactions" value="434"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIR2"/>
    <dbReference type="GeneID" id="18873128"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59906"/>
    <dbReference type="eggNOG" id="KOG2324">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016739_2_2_1"/>
    <dbReference type="InParanoid" id="G3AIR2"/>
    <dbReference type="OMA" id="NCETISH"/>
    <dbReference type="OrthoDB" id="10267474at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050062">
        <property type="entry name" value="Pro-tRNA_synthetase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42753">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME PROTEIN L39/PROLYL-TRNA LIGASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42753:SF2">
        <property type="entry name" value="PROLINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="5">
        <location>
            <begin position="1"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="365" mass="41147" checksum="9AC73345CBDD0B6A" modified="2011-11-16" version="1" fragment="single">MYQITPKFRNEKRPRGGLLRGKEFIMKDAYSFDYSEEQAMVTYDNVVGAYTKIFQDLRVDYVKAAADSGEIGGSLSHEWHCFHETGEDTVFSCDSCKHVSNSEKTLSYPQEVTSVDKVSVRYFMTEDKQTLVCAYYPTKRTLEPKFVKQEIPEIDLKFTNQEEILAEFSNPDTLISKSIIRLMDSRLDSRSNFPDFPVPFVSRSLISMITDIPIVLAEDGEVCERCGDGTLSATRAIELGHTFYLGDKYSKPLGFKIDTPNNEGKLTRNDVMMGCYGIGVSRIIATIAEVYRDSIGLKWPSSIAPWQVTVVEAPGIDSDSSEVTEFYQMLNDSEIDYRRDGRASVSMGKKLKESNMVGIPLSIIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIW3</accession>
    <name>G3AIW3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_134391</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373358.1">
        <property type="nucleotide sequence ID" value="XM_007373296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIW3"/>
    <dbReference type="STRING" id="619300.G3AIW3"/>
    <dbReference type="GeneID" id="18869810"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134391"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_22_0_1"/>
    <dbReference type="InParanoid" id="G3AIW3"/>
    <dbReference type="OMA" id="ISYTGHY"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000013">
        <property type="entry name" value="Related to multidrug resistance proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF78">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="306"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="351"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="405"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="524"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="49"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="567" mass="62733" checksum="601817AB5137785D" modified="2011-11-16" version="1">MAEIDLEKTKTLDDFESQEITIHEEPNELSDKLGDAHLNILPTKKLLVCLGALSLGLFASFADQTSITIALPRIGQDLNAETTINWASTASLMANCVCQVLFGRFADIFGRKQVLLASLGILAIADLACGFAQTGIQFFIFRAFTGIGGGGIQSLTMVMVSDICTLKQRGKYQGILGAQVGLGNAIGPFLMSAFIEKNNWRNFYHMMTPFIVFIMAIIWYLIDTRENTKNLNDVLSRREKFKNIDYLGVFFSTASLILLLIPISGGGSTYAWDSTIVIVMFVIGGICLIVFLIVEWKTTKLPMIPLALFSRLSLSLILGSNFFYGMAYYGFTYYIGYYYQIVRGKDSIHASILMLPLVLPMSISSTIGGVLISYLGHYKYIIIAGYSVWTLSCGLTLLFDVDTSYGVIVVILLVMGCGVGWTFQPTMVAAQSQAKKSERAVVISARNVLRSFGGSVGIAVASLIVSNTLLKQVNQQLNQEPLLLPKSYLVYLKSHIYSVIDTSELTSEQILIVKHMYMKSIRNFFYLTIPLIALCLVSTFFVKDKGLQCIDEVPVDTSKKEDLETKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ29</accession>
    <name>G3AJ29_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small-subunit processome Utp12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_134625</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat PWP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374125.1">
        <property type="nucleotide sequence ID" value="XM_007374063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ29"/>
    <dbReference type="FunCoup" id="G3AJ29">
        <property type="interactions" value="861"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ29"/>
    <dbReference type="GeneID" id="18869841"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134625"/>
    <dbReference type="eggNOG" id="KOG0291">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010458_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ29"/>
    <dbReference type="OMA" id="VYEWQSE"/>
    <dbReference type="OrthoDB" id="3142434at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000920">
        <property type="term" value="P:septum digestion after cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000219">
        <property type="entry name" value="Periodic tryptophan protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000602">
        <property type="entry name" value="Periodic tryptophan protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027145">
        <property type="entry name" value="PWP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19858:SF0">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19858">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="142"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="186"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="335"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="377"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="463"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="7">
        <location>
            <begin position="741"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="650"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="854"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="856"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="902"/>
            <end position="917"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="917" mass="103475" checksum="D7C06148C36125DD" modified="2011-11-16" version="1">MKSDFKFSNLLGTVYRKGNLLFTEDGTKLLSPVGNRVSCFDLINSQSFTFNYQHRKNISCIALNKPGTLLISIDEDGRAILVNAVSRVVLHHFNFKAKVYDIKFSPCGHYFAVAMARFIQVWKTPDVSEDRQFSPFIRHRIYSGHYNDVTSITWSQDSRFFISTSKDMTARIFSLSSDEKDVTMTFSGHRDYVVNAFFSADQEVIYTVSKDGALFKWEYTEKPVESDDEDMEEEEGVAKPMSWRITRKDFFHADGKLKCATFHPQSNLLIVGFTNGEFRIYEIEEFNLIQSLSMGQNSINTVTINKTGEWLAFGSAKLGQLLVYEWQSESYILKQQGHFDSMNTLAYSPDGSRLVTGSDDGKIKIWDVQSGFCLFTFTEHTSAVTGVQFAKRGQVLFSSSLDGTIRAWDLIRFRNFKTFTAASRIQFNCLAVDPSGEVVCGGSQDTFEIYVWSVQTGQLLDSLSGHEGPISCLTFGRENSVLASASWDKTIRIWNIFARNQTVEPIEVTNDVISLTMRPDCKEIAVSTLDGHITIFDIEDAKQLHLIDGRKDIINGRYFDDKFVAKNSGRGKYFKTIAYSFDGLTLLAAGDNNSICMYDIDNEVLLKRFIVSENMSIDGTLQYLNSGKITDSGINSDLIDRDGELSDLEDRIDNTLPGSTRGGDPGERRARPEIRVNSIEFSPTSSAFAAASTEGLLVYSINQDVIFDPFDLDVDVTPEATLESLNDKEYLTALVMAFRLNESYLIHKVLESVPLSDIQLVCQDLPVVYLNRVLTFIGELLIKQNSPHFEFYLLWVKNLLIQHGKYISGHRLELQSSMKLLSRFLNKIAKDVVTVGKKNDYLLSYLTVSKDLKEDVPEEEQGSEESEGEDVVMDEDEDSDDEGWLLPNEHSLKSGNGIVFEVDGDEDSDEDDELIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ37</accession>
    <name>G3AJ37_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_65675</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374133.1">
        <property type="nucleotide sequence ID" value="XM_007374071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ37"/>
    <dbReference type="GeneID" id="18875056"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65675"/>
    <dbReference type="eggNOG" id="ENOG502QW7F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_785655_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ37"/>
    <dbReference type="OrthoDB" id="4073891at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="40509" checksum="147702801C4464AD" modified="2011-11-16" version="1">MCLFSSMSIKIVAKTKKNFPATLLFNKMDNQDLEKSPKTSTEFTNDPPPALPQSHLPLLNYTFIENISSLLIIISSIVYFSGNYSIFKFKVNVNSSYSQNIISIVQEGLKFITYLSNDLNVYVDETISDSNLQQIDSCYDNNLYQFNIWGFCRYDQFGGVVNCYKGQGLNMIACFIQDFGMQLGNLTNQPDPQAVSSRFVDVYYKSIQQLCVDTPTKLQDYQSQICKLQAYNIGGLFVQQVAVFHYGCLVVLFILGVAEQVFVSKYGFHRLRLNTRLAAKIVWLFLCFMSAHLIYIINSTMQLPIGYLLQNYPVGILYFEDWKFVLWNVIFLVNTVIGIYVGAKKRKIERDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJB5</accession>
    <name>G3AJB5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 18">3-isopropylmalate dehydratase</fullName>
            <ecNumber evidence="6 18">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 18">Alpha-IPM isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17 18">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">SPAPADRAFT_134446</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 18">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 18">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="6 18"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374158.1">
        <property type="nucleotide sequence ID" value="XM_007374096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJB5"/>
    <dbReference type="FunCoup" id="G3AJB5">
        <property type="interactions" value="240"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJB5"/>
    <dbReference type="GeneID" id="18869820"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134446"/>
    <dbReference type="eggNOG" id="KOG0454">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006714_1_0_1"/>
    <dbReference type="InParanoid" id="G3AJB5"/>
    <dbReference type="OMA" id="EDNEPHT"/>
    <dbReference type="OrthoDB" id="2279155at2759"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009316">
        <property type="term" value="C:3-isopropylmalate dehydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000006">
        <property type="entry name" value="3-isopropylmalate dehydratase large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000007">
        <property type="entry name" value="3-isopropylmalate dehydratase large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000003">
        <property type="entry name" value="3-isopropylmalate dehydratase small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01026">
        <property type="entry name" value="LeuC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01031">
        <property type="entry name" value="LeuD_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004430">
        <property type="entry name" value="3-IsopropMal_deHydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004431">
        <property type="entry name" value="3-IsopropMal_deHydase_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012235">
        <property type="entry name" value="3-IsopropMal_deHydtase_ssu/lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050067">
        <property type="entry name" value="IPM_dehydratase_rel_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00170">
        <property type="entry name" value="leuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00171">
        <property type="entry name" value="leuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002458">
        <property type="entry name" value="PRK01641.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004016">
        <property type="entry name" value="PRK05478.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009116">
        <property type="entry name" value="PRK12466.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822:SF9">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001418">
        <property type="entry name" value="ACN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10 18" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="15 18" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 18" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="14 18" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="19">
        <location>
            <begin position="8"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="20">
        <location>
            <begin position="536"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR001418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EGW34574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="766" mass="84491" checksum="9F1D3281F45EB542" modified="2011-11-16" version="1">MSPKTLYDKVFEDHIVHKDDSGSYLLYIDRHLVHEVTSPQAFEGLKNAGRKVRRTDCTLATVDHNIPTISRANFKNVETFIDQDDSRLQVSTLEANVKEFDVTYFGMSDDRQGIVHVVGPEQGFTLPGTTVVCGDSHTSTHGAFGSLAFGIGTSEVEHVLATQTIIQAKSKNMRILVNGDLAPGITSKDLVLHVIGVIGTAGGTGCVIEFAGKAIESLSMEARMSICNMAIEAGARAGMIKPDQITFDYIKGRPLAPKGEQWDKALAYWKTLHSDEGAKFDYDIKINAADIVPTITWGNSPQDALPITAKVPDPANVTDPIKKAGMERALQYQGLTPNTPLQEIKIDKAFIGSCTNSRIEDLREAAKVAKGHKKADNVKLVLVVPGSGLIKAQAEREGLDKIFEAAGFSWREAGCSMCLGMNPDILDPEERCASTSNRNFEGRQGARSRTHLMSPAMAAAAAIKGHFTDIREFDYVNHDEPAVHIEQEQEDKELQDAVYEHEKEYIEETPGIENDRLNDIPKPTTQSAGVSGAVDKFTVLTGITAVLDKANVDTDAIIPKQFLKTIKRTGLKAGLFYEWRFNQDPVTGKDIATDFVLNVDPYTHAEVLLVTGDNFGCGSSREHAPWALKDFGIKCIIAPSFGDIFYNNSFKNFLLPIRIPQEVILEKLVPVVKQGHKLTIDLPSQQIKDGETGEVIIDNFDVEEFRKHCLVNGLDDIGLTLQKEEFIVKYEEKRREKFSFLEGGSKLIKPIKGTKKSPYGNKAQEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJH4</accession>
    <name>G3AJH4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_59252</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373461.1">
        <property type="nucleotide sequence ID" value="XM_007373399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJH4"/>
    <dbReference type="GeneID" id="18872837"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59252"/>
    <dbReference type="HOGENOM" id="CLU_2856102_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJH4"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="65" mass="7513" checksum="E7BA227FF9FEB07A" modified="2011-11-16" version="1" fragment="single">MQDSLNRPINGKRRHVMNDVFSRVFPLMSRIIEIMETDVKPMASKGGHSSRRECVDFSCINPLYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJP1</accession>
    <name>G3AJP1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mss4-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_54194</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373526.1">
        <property type="nucleotide sequence ID" value="XM_007373464.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJP1"/>
    <dbReference type="FunCoup" id="G3AJP1">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJP1"/>
    <dbReference type="GeneID" id="18871844"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54194"/>
    <dbReference type="eggNOG" id="ENOG502SGHA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124782_1_0_1"/>
    <dbReference type="InParanoid" id="G3AJP1"/>
    <dbReference type="OMA" id="GPIGMVC"/>
    <dbReference type="OrthoDB" id="30840at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.10">
        <property type="entry name" value="Metal Binding Protein, Guanine Nucleotide Exchange Factor, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007515">
        <property type="entry name" value="Mss4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011323">
        <property type="entry name" value="Mss4/transl-control_tumour"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13276">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR MSS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13276:SF0">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR MSS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04421">
        <property type="entry name" value="Mss4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51796">
        <property type="entry name" value="MSS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="16585" checksum="0D52F03455BE2E0B" modified="2011-11-16" version="1">MPLELSSEIDLEKLQSEDHNVILRCPFKQCNTRIIKYSPSFIQISVDNAPQTVKATKDELPTIERPTNFYQIDNVWDFDNIGVSRPSSISDDPIIGHDSTVKIDRLLICSECDKGPLGFAGIPTDKEHHHTNLKYFLSCNSTMYEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJP6</accession>
    <name>G3AJP6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_65146</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373531.1">
        <property type="nucleotide sequence ID" value="XM_007373469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJP6"/>
    <dbReference type="GeneID" id="18875022"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65146"/>
    <dbReference type="HOGENOM" id="CLU_622827_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJP6"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="387"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="395"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="440" mass="49284" checksum="BF6DA5770347086C" modified="2011-11-16" version="1">MKSYHFDLYGQPFGFHRSKFFGKKDFESPILEAIITKLDVSSDKNSPFVNTDTTQSLHNTEKTATVETTTAVGTIVVAAEGSDATVKQSKRLRFKKWLEKAKSKGFIVDFSKIAAVDSSSNSNLDDYVCSSNDTNTIKAASDNTPNTTTAATNSSKLTDTTPKIITVTTPSISVTRPPLVSGSKREKVKGWFTKIIYGNLQTDSENVALKESVVDDKDANDEVIFVDYFTCSSTDLKDDHFKTVSPPEIHISKRHQLKQWFKGFFIYPQASSDDQNIPDPDLVDEHENVDFKSFDSIFLHGPPSSCSNDDANSFKVKPSFPSIFYTAVTESAPLLFQRLKDWFIRLVFGKGKSRDVDSTEDNDYADKDSDDVDLGVESEISLDVQGQTVLEDNGDITPNNDNTNDNISPDAETEDDDFEEIWVYCFERTLPYKKRVPKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJR8</accession>
    <name>G3AJR8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_134094</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373553.1">
        <property type="nucleotide sequence ID" value="XM_007373491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJR8"/>
    <dbReference type="STRING" id="619300.G3AJR8"/>
    <dbReference type="GeneID" id="18869781"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134094"/>
    <dbReference type="eggNOG" id="ENOG502SDTR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_782039_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJR8"/>
    <dbReference type="OMA" id="DKFANIW"/>
    <dbReference type="OrthoDB" id="4013163at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442556" evidence="1">
        <location>
            <begin position="18"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="355" mass="40793" checksum="C53CA25A8D119BFD" modified="2011-11-16" version="1" fragment="single">MKFILCFILLHILSVSSLLLPQEWSVVRPTLENLRETKTRLSTSLQSLISDHTYENSSIHETLTFIPRELMHPALFSNLGLSYLFEGKQQWVNLTSVDQVVVPKDFQSFFIFNSSEPVPSSNFKGPGFHKLLGRSVPFALPHWLELSDYYMVVPSSHLNLSSSSELVTLPRSKLPIFRESVTGNKPMLVFMFNQTTPRSKHTPIITIKNYLTSEGRFNSISSFFDRFANLWLTRVNYDNLVHAVYCTLTSSESYCLKIKDDQSVHVFPALHDFPRHFHSKFGDKREIETQAIRQIHDVLSVKTDKFNDISYELAHSPKTVIEKADEKLDNKLDGTITRIHDVKESVLDKLDGRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJX9</accession>
    <name>G3AJX9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">GTP-binding protein YPT32/YPT11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_59446</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Golgi apparatus membrane</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373614.1">
        <property type="nucleotide sequence ID" value="XM_007373552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJX9"/>
    <dbReference type="FunCoup" id="G3AJX9">
        <property type="interactions" value="1002"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJX9"/>
    <dbReference type="GeneID" id="18872919"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59446"/>
    <dbReference type="eggNOG" id="KOG0087">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_23_2_1"/>
    <dbReference type="InParanoid" id="G3AJX9"/>
    <dbReference type="OMA" id="ITAIYQM"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000067">
        <property type="entry name" value="ras-related protein RABA1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="187"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="222" mass="24757" checksum="C5C8075A9AD16C3F" modified="2011-11-16" version="1">MADNTDDYSYDYEYLYKIVLIGDSGVGKSNLLSRFTRDEFNLESRSTIGVEFATRTLEIDGKRVKAQIWDTAGQERYRAITSAYYRGAVGALIVYDIAKTESYESVSRWLKELKEHADSNIVIELVGNKSDLDHLRAVPTEEAKNFAQENNLLFIEASALSSDNVDLSFHQLLKNIYEMISKHQLDTNEQGGVNKPTTAGGPTISLTPAPQSKKNKNNGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK58</accession>
    <name>G3AK58_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Proteasome activator BLM10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_150198</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BLM10 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374384.1">
        <property type="nucleotide sequence ID" value="XM_007374322.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AK58">
        <property type="interactions" value="355"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK58"/>
    <dbReference type="GeneID" id="18870857"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_150198"/>
    <dbReference type="eggNOG" id="KOG1851">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000772_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK58"/>
    <dbReference type="OMA" id="ECTQLVP"/>
    <dbReference type="OrthoDB" id="17907at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016504">
        <property type="term" value="F:peptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032430">
        <property type="entry name" value="Blm10_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032372">
        <property type="entry name" value="Blm10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035309">
        <property type="entry name" value="PSME4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021843">
        <property type="entry name" value="PSME4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32170">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32170:SF3">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16547">
        <property type="entry name" value="BLM10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16507">
        <property type="entry name" value="HEAT_PSME4_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11919">
        <property type="entry name" value="PSME4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Proteasome activator Blm10 N-terminal" evidence="8">
        <location>
            <begin position="103"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator Blm10 middle HEAT repeats region" evidence="7">
        <location>
            <begin position="539"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator complex subunit 4 C-terminal" evidence="6">
        <location>
            <begin position="2084"/>
            <end position="2172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="214"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1086"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="218"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1086"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1126"/>
            <end position="1139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2172" mass="248537" checksum="29BE044A349AB7F3" modified="2011-11-16" version="1">MPPPDKPSTNLPGKQPAVGNGQQGNSVPKVNVTAPIPIKSLHQHHNQLQQHIEGLITPPTRVASSFQIRQHAFDSETTLRSFTPSTISKVTKSTARRPSYGTIVSDREHYFNLDYPNDPELLSKLQLDPKSRYFARSRPRTLDLPSLLPYKIEKPEEQAKFLSHIISHLYIAIKTLDLQGSLSVSAKDLAALKNVSGISDVDLALETNLFEMSTHDASEEEDFSEMDLETSDSEGEEDDEEEEGEDEDMDGDRETTIQHKKSPKSAAVVGVRTWTHELLVWLKMKYNMPVTIRINLAKVYYAICLSRGQHINLKTYVRVFELLTKEQSLLYNQGLRLPWDGLCKELEHHFPSVDSGLVAFEKKDHKQLLRLASRASYFFEKDCLPVIYKRLASQFSVTNSSMVMSSLSILPSFFTSQGKENENDIRYYIQSLFYMWLKLNRIQGFDAQVTSRLGVISMSVLSHLNDNPSSMNYLKLGKFGLYTEEQMEFITNTLINSLSIMVEKYSSMKSKYFHGFASMIIFSMIGDSALEDGGILYYLRTLTNAIQSYIHPSNTGDWTRPISKLIIALVYQFHKRYNSETQPDGFYYNIPQEVKLSSKVVKEFVAIFLPIVKIGIQSKKTNATEDYLSTLQLLAFLDPEVVLESTLLDIYESLEGVISTHRVSIALRSIDTLSRFLVSTPIYRVHVTRLLSLALPGIDSNDLEKTIQTLEAFAAIANYAPYYDLTKAEGDPTLAMEFTQDHIEYLKRKMYQVDEEEFHVDPELEVAALKSSTTAFKDLFKSLTERLTLLIENLPDPNKSSGLEHDLATSLPKFFYILFEAMSDDLFIHFKNEFFDFVFNNTYHTMSHVVGEICGGIIKRYPKCFKKTARLLIEKIHQEIEENGAGGTARGDGAASDIVPRDQPLYWTLSILNECIGNAGEKIVDMSTELTDLSYYLMENIKGPIVFTSAYLVNQILQSVSKIRITECRLISPQYEEKFGVDEKCWGGFWNDSRRFNKENTTFDWFIPTEREIVFAVEFFNGHVTKTLENITSLIKQFNKDDVKLSMKLTDDLRKNLLYLAYLLSGVSYLLDPSFEEDIPKLSADKESIQQHHQPDHHDLDHDKEMDDGRTSKPSTPDHLAFRFSSHLNELSRPQSTAPTYEGSGRDTPQIDGINMSSVNPAITFRERSLYTSNYFFGNDTENRQHRDLYLKIHKTRHLIGRSLHIIYKFLVENFRDNTKAFKNFLYVVNMWFSDVGRERILDSSHARISYGYVSYIQTINRIRKPFTRMAIGARLEAYHSFRVALHATSRTETNLDKILLEDVVKLSVSTYSNIARNAQGILIDGMKRLNGSYTLIIRSAFKYLTKALDANDHKKIESGLSIFAIRKIKSKLQSDFFNIYKYIDLLHRCLQIDNAEVHSLAEQLFSGTSKGITPPSRPCLIDHEAIDTIRPPDQFIDLEIRAVKLAKEKKRKVYFDKLSKLQDKVLSDESNNSHWKTTLMNLELLVNIQSYLEVATRSDVLQLLQKEASTEHPTISRFALKGITRIINKLAVLHGFEYDFEKAYDLNYIPKNLKVIDTRPKNDVSYTSVWREEMKNSSNPTYYVDYKASVGWLFWNDSMLAMSNEPFFKLDLNPEDLQAMMGFSISLTKNWFLRIVKLWVTDNDANSAFQGTDVFITAALVLLISNGYTENFTFDEMLSVVDEVYVPDDKSAHIVTCELLAGILIGSKFMDPKYATKRDDFISSYLKRILNHDLSPDTSGIWNIFSWWIPAHIDCRRFPKVINEIFEFKLDPNSDSAFREATRISYIRSIASSVSWCIPDPDKLLELCIMNINHRYAAVRSQIGSLMAVLSFPYYAECLESSDDFLTSANTQSKLMLYQDQDVGSLMNLVPNLFRQIESWRLEVHQLTPQEILKSNYIYTATTVLTWLRQVLSTSAAILFERHVTTELVPFLLELINMREVCQLGNIDPITVLKKVSQIPYTVKCLDSIIDMLESYSDRDLNLVQKLVMGEFTETVFFKNLFNLSKLQRERFIVLTDKMLHHKQVEIREAAANTLSGLIHISPPGEVEQLVVTRSQAYSSELDKIRKKYRKSGYKNMNQVDVIDLHGATLGLGALVHAFAFSSPPPKWVPGILTTLANKCTGIPGLVGKTAKETLSKFKKTRQDTWHIDSIVFNEDQIQDLEGVLWKSYFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK84</accession>
    <name>G3AK84_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Choline transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60882</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375058.1">
        <property type="nucleotide sequence ID" value="XM_007374996.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK84"/>
    <dbReference type="STRING" id="619300.G3AK84"/>
    <dbReference type="GeneID" id="18873615"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60882"/>
    <dbReference type="eggNOG" id="KOG1289">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004495_6_2_1"/>
    <dbReference type="InParanoid" id="G3AK84"/>
    <dbReference type="OMA" id="KWQLFVI"/>
    <dbReference type="OrthoDB" id="2417308at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901235">
        <property type="term" value="F:(R)-carnitine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015220">
        <property type="term" value="F:choline transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034228">
        <property type="term" value="F:ethanolamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031460">
        <property type="term" value="P:glycine betaine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000046">
        <property type="entry name" value="Amino-acid permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF7">
        <property type="entry name" value="CHOLINE TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="247"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="334"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="384"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="448"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="485"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="534" mass="59408" checksum="2E32DF8EF541B772" modified="2011-11-16" version="1">MDSDKVLADNASFEAKDFQETNEKYSDHEDSSSLDRVHHEGGKLKRRFSTYSIICIGFGLTNSWLGISSSLVTGISSGGPMLTIYGILIVAMISYCVAVTLGEMSSAIPSAGGQYVWTRVLAPKKYSSFLAYICGSMSWAGAIFTSASMSAALAYQVLGFWNLLNPDRARFDQWTYFVIYEIVTIILFFFNSWGKILPILGNGALYVSLFSYTVILITVLVCSRGNYQSPHFVFVQFNNGTGWKSSGIAFIVGLINPAWSFSCLDSVTHLSEETSKPRTDIPKAVLSTVTIGFVTAFTYSIAMFFCVTNLEELTNSQTGMPILDIFYQALKNRQGAVCLGFLVFLTAFGCTVACHTWQTRICWSFARDNGLPFSKYLARVDPKLGVPINAHIFSTFWVMICGIIFLVTTAGFNAMIVGTITLILGSYMVFCFCLLYRGRNNIKHGPFWLGKFGYFCNIVVILYGIFAFVFFSFPATMPVTAGDMNYFAVVFVIYVILALGYWWFPVKQWSARVNFAGGRHGEEIEFPEVCLTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK88</accession>
    <name>G3AK88_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vacuolar protein sorting-associated protein 54 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_151822</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS54 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375062.1">
        <property type="nucleotide sequence ID" value="XM_007375000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK88"/>
    <dbReference type="FunCoup" id="G3AK88">
        <property type="interactions" value="456"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK88"/>
    <dbReference type="GeneID" id="18870907"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_151822"/>
    <dbReference type="eggNOG" id="KOG2115">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003094_0_1_1"/>
    <dbReference type="InParanoid" id="G3AK88"/>
    <dbReference type="OMA" id="QKQAVML"/>
    <dbReference type="OrthoDB" id="10259024at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039745">
        <property type="entry name" value="Vps54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012501">
        <property type="entry name" value="Vps54_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12965">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12965:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07928">
        <property type="entry name" value="Vps54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 54 C-terminal" evidence="9">
        <location>
            <begin position="705"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="459"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="963"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="461"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1000"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1000" mass="114523" checksum="75F14EA19876916C" modified="2011-11-16" version="1" fragment="single">MPRTSIDNESIFSSSAHVSAFNPDETKDDIYSPLGPNSIYELTIGSDLARIRRNRPPKSSVTINGGVNTVYNLSTPSTRDIPQIQLSKLKSKVTDADLYEKYVKNAEREYKQFESSYKLLTEDTLQKFQQQQQQQQRISASSSVEDIQIIDSISDIPKVFLDSNFRLDDPRIFKQVIEDTSILGESSLVNNTDLQDKLSNYLDIVEMNLIQEIGKSSDSFFNAIGDIEKIQIQSKSCVDIFNNITKELEELEQAQSKRGLKIMHKLIEKKNVETFESTLIQIQYITSFFNLSNKSFTDGNYAKCLNEIVVVEKLLHGVRYQDVTDPDLKNLYPDLPNLVELTQLPAFIHLRNDLQSLKHECSKGYTDDFVELLTTDLRDHYRNVATKDTLNRLYVNKDKTKKYASRPVIASYLNIEEEKKSKLKEYVRDLEKAGNLSEAYSTYQHRFITEIKDIIKQNLPSSQSQEMSRTSSRASPAPPDTGKPNALSTNIKTLTPREFENMFSKTCAELSECLRRLTAHQKLLLDVALTSISSTNDIDIMSLDITTAIHKAIELTQIRLMKVISVRSEQTADLPVQFYLRLYSITSSYLQECEMINPGFAFGGAGNALGEWFSNHLTYFVHRFHINSIREMSTECTKEIWREVVDPKELAAAQLAIDELIGYATGESGDKLNEYFDFYEDNEKEPQEQLVDETVRKLSISTDVFLVPSLALKNVYHIRDYIVIAKAFPSFASTITNNLLNYFKVLNSKTSQAVLNAGATRTAGLKHITTKHLALCIQFVEFNIGLLEATKNFFPQDTHQENPEEITYSRILSNYKDHETELFSKVVTMMHDRTVMRCAGLPKINWSEPIKHPQQCHSYMEDLVKDTITVAKVLTKYLPELKSSLILSQIFDNYKRLFVESYCTQLPPFKDINEKQNVLKDIDYFRVKLFDVGGYGNSGQVIWENVNSMTTEEEAKMEEIMRHNIQDEQKQKSPPLRPEAPARRFSFDRLVSSARSSFDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK93</accession>
    <name>G3AK93_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">cAMP-dependent protein kinase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_60888</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8 9">Tetramer, composed of 2 regulatory (R) and 2 catalytic (C) subunits. In the presence of cAMP it dissociates into 2 active monomeric C subunits and an R dimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the cAMP-dependent kinase regulatory chain family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375067.1">
        <property type="nucleotide sequence ID" value="XM_007375005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK93"/>
    <dbReference type="FunCoup" id="G3AK93">
        <property type="interactions" value="436"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK93"/>
    <dbReference type="GeneID" id="18873619"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60888"/>
    <dbReference type="eggNOG" id="KOG1113">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018310_0_1_1"/>
    <dbReference type="InParanoid" id="G3AK93"/>
    <dbReference type="OMA" id="MPYERSK"/>
    <dbReference type="OrthoDB" id="417078at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046580">
        <property type="term" value="P:negative regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097271">
        <property type="term" value="P:protein localization to bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010603">
        <property type="term" value="P:regulation of cytoplasmic mRNA processing body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd12098">
        <property type="entry name" value="DD_R_ScPKA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000039">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000118">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012198">
        <property type="entry name" value="cAMP_dep_PK_reg_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003117">
        <property type="entry name" value="cAMP_dep_PK_reg_su_I/II_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02197">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000548">
        <property type="entry name" value="PK_regulatory"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00394">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47391">
        <property type="entry name" value="Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0114">cAMP</keyword>
    <keyword evidence="4 9 10" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="12">
        <location>
            <begin position="224"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="12">
        <location>
            <begin position="342"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="85"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="108"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000548-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW33552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="464" mass="50856" checksum="EB5DCDEC28BF9FD5" modified="2011-11-16" version="1">MSHQQQQQFITEELSQLQREILTKNPTDILQFCANYFNSKLQAQRTHLWEQQAKANAAGIDLFPPVTNISLNASGIGFLQDRQPSFKSPFGLNDPHSNHDDDPHAPASNFNPQQSTQAPQSANSEPAGSLFKSNFDGTASSSSKIQKEVDPSSPNGESSSHPSALPFKPAGKIPIAFNANRRTSVSAEALNPAKLKSESWKPPVNNLSAEEEATLAQNLKNNFLFKQLDAQSKRTVLAALSSRHFPKDTVIIKQGDEGDYFYIIEKGTVDFYVNDAKVNSSGEGSSFGELALMYNAPRAATAIAATDVTCWALDRLTFRRILLEGTFNRRLMYEDFLKGVKVLESLSDHERSKLADALWTEMYHAGDKIVTEGDQGENFYLIESGNCDVYSQSGGHLARLTKGDYFGEVALLNDLPRQATVQALDNVIVATLGKSGFQRLLGPAVDVLKAQDPTKSHDAATTVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKI1</accession>
    <name>G3AKI1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MINDY deubiquitinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_60278</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374452.1">
        <property type="nucleotide sequence ID" value="XM_007374390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKI1"/>
    <dbReference type="FunCoup" id="G3AKI1">
        <property type="interactions" value="289"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKI1"/>
    <dbReference type="GeneID" id="18873313"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60278"/>
    <dbReference type="eggNOG" id="KOG2427">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022566_0_1_1"/>
    <dbReference type="InParanoid" id="G3AKI1"/>
    <dbReference type="OMA" id="HTRFSNE"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016807">
        <property type="term" value="F:cysteine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990380">
        <property type="term" value="F:K48-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007518">
        <property type="entry name" value="MINDY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033979">
        <property type="entry name" value="MINDY_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18063">
        <property type="entry name" value="NF-E2 INDUCIBLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18063:SF6">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04424">
        <property type="entry name" value="MINDY_DUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MINDY deubiquitinase" evidence="2">
        <location>
            <begin position="7"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="396" mass="45246" checksum="66D9649D709D6DCB" modified="2011-11-16" version="1">MPDSVKFNIKLVKWSLDYDSSDSNDLISTPIVLQDKNGPCPLIALINTLMLSNDLNLSRSDYIPSNQAKSDAILHFKSVLLQHESVAIELDLLLKHLGDLLLEFTQDNKSGLRFEIDQLLNSLPLLHTGLTVNPNLINGEFNPEDLSTSLFTVFDLKFRHGWVINQIDNENIEWNEEAAPQEGSSSSHADNYAQVVELFNELQTFDRIQDYLLLDSTKDPKLAHNQTLIHKWLDLNRTQLTRIGLDKLNLCLVNEQFVVLFRNNHFSTMFKKSDKEFYVLITDTSFQTSKSSSKIIWQSLNSISGNDDLFFTGDFLPVLNVDQEPQEQYDTDYLLTKQLQEEEDEALAKRMQQSYDKKLVKSENQAKEQKTPTPAPKPIAKDAPKKKKKKSVCVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKU3</accession>
    <name>G3AKU3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptide-methionine (S)-S-oxide reductase</fullName>
            <ecNumber evidence="2">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Peptide-methionine (S)-S-oxide reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60979</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(S)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:14217"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12315"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:44120"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[thioredoxin]-disulfide + L-methionine + H2O = L-methionine (S)-S-oxide + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:19993"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58772"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="2"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375159.1">
        <property type="nucleotide sequence ID" value="XM_007375097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKU3"/>
    <dbReference type="FunCoup" id="G3AKU3">
        <property type="interactions" value="584"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKU3"/>
    <dbReference type="GeneID" id="18873666"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60979"/>
    <dbReference type="eggNOG" id="KOG1635">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031040_10_2_1"/>
    <dbReference type="InParanoid" id="G3AKU3"/>
    <dbReference type="OMA" id="LFWESHD"/>
    <dbReference type="OrthoDB" id="77405at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1060.10:FF:000006">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="entry name" value="Peptide methionine sulphoxide reductase MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01401">
        <property type="entry name" value="MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774:SF1">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE MSRA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide methionine sulphoxide reductase MsrA" evidence="7">
        <location>
            <begin position="19"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="186" mass="21106" checksum="450D9C0F7492F46F" modified="2011-11-16" version="1">MSVSTISSTLKTTPTSQLITLAAGCFWGVERVFKKQFENKGLVDVKVGYANGIPTVGNVNYELVCSGSTNFVESIQLSYEPAQLKLEDILDIFFRMHDPTTVNSQGPDVGTQYRSAILTHNDQDNALAWKLKEEYQKTWYPNDKIVTIIEPIKVWHDAEEYHQKYLEKNPAGYECPSHFIRTKPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL74</accession>
    <name>G3AL74_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_60428</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374632.1">
        <property type="nucleotide sequence ID" value="XM_007374570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL74"/>
    <dbReference type="FunCoup" id="G3AL74">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL74"/>
    <dbReference type="GeneID" id="18873403"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60428"/>
    <dbReference type="eggNOG" id="KOG0255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008455_11_1_1"/>
    <dbReference type="InParanoid" id="G3AL74"/>
    <dbReference type="OMA" id="CYQAHAY"/>
    <dbReference type="OrthoDB" id="3357846at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015244">
        <property type="term" value="F:fluconazole transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00880">
        <property type="entry name" value="2_A_01_02"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF23">
        <property type="entry name" value="FLUCONAZOLE RESISTANCE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00173">
        <property type="entry name" value="EDTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="332"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="396"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="1"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="434" mass="47333" checksum="8F3E32860A0B71BB" modified="2011-11-16" version="1">MGSAVYTPGIEQIMEEFGIGSVVATLPLTLFVLGYAIGPLVFSPLSENAAFGRNSIYIYTLALFVILQIPTALVDNIAGLCILRFLAGVFASPCLATGGATVADVLTPPYVPVGLASWALGAVCGPSFGPFFGSILVVKGGWRWTFGFMAIVSGFALIVLSFTFPETYGKTLLYRKAMRLRGITGNQRITSEGEVENRSKTTSQIAVETLWRPIEVFIKEPVVLMINMYIALVYSIFYLFFEVYPIFFQEIRGFTLIELGTTYFATIIGIVIAAVIYIPIVYQVFTKRILNGKGVQPEVFMPIAIVGGVLMPVGLFIFGWSATTHTHWIGPLIGGAIFAAGGFLIFQTMFSFIAGSFMYVGSIFAGNNLFRSITAGCFPLFGRPLYNNLATKEFPVAWGTSILAFLALAMIAIPVLFYLNGPRLRARSKFALQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALB4</accession>
    <name>G3ALB4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Gluconokinase</fullName>
            <ecNumber evidence="3 9">2.7.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_60463</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>D-gluconate + ATP = 6-phospho-D-gluconate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19433"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18391"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Carbohydrate acid metabolism; D-gluconate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the gluconokinase GntK/GntV family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.12" evidence="3 9"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374672.1">
        <property type="nucleotide sequence ID" value="XM_007374610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALB4"/>
    <dbReference type="FunCoup" id="G3ALB4">
        <property type="interactions" value="1114"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALB4"/>
    <dbReference type="GeneID" id="18873425"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60463"/>
    <dbReference type="eggNOG" id="KOG3354">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077168_5_0_1"/>
    <dbReference type="InParanoid" id="G3ALB4"/>
    <dbReference type="OMA" id="YEGDDYH"/>
    <dbReference type="OrthoDB" id="275177at2759"/>
    <dbReference type="UniPathway" id="UPA00792"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046316">
        <property type="term" value="F:gluconokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02021">
        <property type="entry name" value="GntK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006001">
        <property type="entry name" value="Therm_gnt_kin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01313">
        <property type="entry name" value="therm_gnt_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43442">
        <property type="entry name" value="GLUCONOKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43442:SF3">
        <property type="entry name" value="GLUCONOKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="134" mass="14837" checksum="30401CA769FEE5FB" modified="2011-11-16" version="1" fragment="single">MASTVIVVGGPAGTGKTTQAQLLATQLKCAFIEGDDLHPQANVDKMARGVPLTDEDRWGWLKQLSSESASTAKGDTANVCVVTCSMLKRVYRDYIKKCAKTAAGEDDVKFRFVFLYTEFNELLKRVEGRKNHFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALH8</accession>
    <name>G3ALH8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_136319</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374736.1">
        <property type="nucleotide sequence ID" value="XM_007374674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALH8"/>
    <dbReference type="STRING" id="619300.G3ALH8"/>
    <dbReference type="GeneID" id="18869957"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136319"/>
    <dbReference type="eggNOG" id="ENOG502S9S7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126658_3_0_1"/>
    <dbReference type="InParanoid" id="G3ALH8"/>
    <dbReference type="OMA" id="NHIESEQ"/>
    <dbReference type="OrthoDB" id="5778525at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057072">
        <property type="entry name" value="bHLH_INO4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10328:SF11">
        <property type="entry name" value="MAX-LIKE PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10328">
        <property type="entry name" value="PROTEIN MAX MYC-ASSOCIATED FACTOR X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23181">
        <property type="entry name" value="bHLH_INO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="4">
        <location>
            <begin position="31"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW33221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="119" mass="13376" checksum="B1C5B6F186E82117" modified="2011-11-16" version="1">MSENSSESPSPSRKNGKKAKTSKSSVLSEEQKKAHHIASEQKRRENIRSEFDKIVSLTPTLTESENRSELNILTKSADYIDELREENVKLVELCRMKGINVPQELIYNGPQNDGSDIGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALR2</accession>
    <name>G3ALR2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome production factor 2 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ribosome biogenesis protein RPF2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_50200</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
            <location evidence="1 4">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the RPF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374820.1">
        <property type="nucleotide sequence ID" value="XM_007374758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALR2"/>
    <dbReference type="FunCoup" id="G3ALR2">
        <property type="interactions" value="1031"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALR2"/>
    <dbReference type="GeneID" id="18871415"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50200"/>
    <dbReference type="eggNOG" id="KOG3031">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049783_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALR2"/>
    <dbReference type="OMA" id="VGLKPMF"/>
    <dbReference type="OrthoDB" id="407658at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039770">
        <property type="entry name" value="Rpf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12728">
        <property type="entry name" value="BRIX DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12728:SF0">
        <property type="entry name" value="RIBOSOME PRODUCTION FACTOR 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="6">
        <location>
            <begin position="28"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="292"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="300"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="335" mass="38707" checksum="ED768DFDB016742D" modified="2011-11-16" version="1">MIRTIKPKNARSKRALAKKEAKLVENTKSALFVPGSTGNKMLHDAMCDLMALKKPHAKKFSRKNEIRPFEDAAELEFFSEKNDSSLMVFSSNNKKRPNTLIFTRFFNHKVYDMIELSIQQNYKLLQDFKKTTFTIGLKPMFVFNGPAFDSHPVFQHLKSLFLDFYRGEETDLQDVAGLQYVIALSVGEIEDLNASDKNLPLLHFRVYKLKSYRSGQKLPRIELDEIGPRFDFKIGRRITPAPEVEKEANRKPKQLEAKVKKNVSTDFMGDKVAQIHVGKQDLGKLQTRKMKGLKSKYDQLSEDEDEEEGEYEEFADEIESEDEVEDEPRAKKQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALV2</accession>
    <name>G3ALV2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_136141</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374226.1">
        <property type="nucleotide sequence ID" value="XM_007374164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALV2"/>
    <dbReference type="FunCoup" id="G3ALV2">
        <property type="interactions" value="1214"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALV2"/>
    <dbReference type="GeneID" id="18869942"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136141"/>
    <dbReference type="eggNOG" id="KOG1128">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004905_0_1_1"/>
    <dbReference type="InParanoid" id="G3ALV2"/>
    <dbReference type="OMA" id="NNRYARA"/>
    <dbReference type="OrthoDB" id="1936594at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044244">
        <property type="entry name" value="TTC27/Emw1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="921" mass="104555" checksum="A920728D0DB58FBD" modified="2011-11-16" version="1">MDTLRNQLAQLVLLVPPESFKVNADDSVGSAILQILNGDYTDLLEHDYFFHQLLSVPELAEITSKYPNTSLIEVLVEYVGALDKLLNDKVKLHLLAVCFLQLFIQSNYTGPSVKISVQKELFGGSEQVHSDAVRLLSLEGQQAYDLMQEPLYLVLAELIFENLVGTPFELSLFNKDVTKNTEKLVDFTQETQAQHKDDAVYASIFWWKVRTLQVHLSVVSEPPSLISTVSGILLHNTIATALAPSGTSDPELQRLVQIELLLERARIHIHAQTEHSSISPLAQVKRISEFNFLLSGAKAKRTKFQKYHNSVLLVLAKSNRTSVYDTESTKESPESFNLDSDLLLERPQFESLDDLEMKLEPDVKRIKFDTAEQESQEDKLLPIAIRQDDIPADLKSLDPNNQPGLSNIDNLQLLLRLTVLKQTSPAKDPLVEEELMALVSRVLYSTEGNVNWLIFSRALWERSLLETEKSRTVERGILQMTSLIEEIGLKIKTKMIPEAMTDDPNAITAQRLRFIHQLPLLPQWSMDVKLAEKYMSLGILKSALDIYERLQLPVEAALCYAAVNNEAEAERILIERIKTHPDDARAISILGDIKQDPELWLMAWEIGRYAKAKASLSKYYHDPPASAGIPRNIDLSIQHMHDSLSAYPLSYENWFFYGCCGLESQQFELASEAFTRCVALDDTNSYAWSNLASALLKLDKTKPAFNALQRAVRCSGENRKSWRIYENYMIVAAKLHEWNDVLIAAREMIDIKKGEGDAAIDIPVMEKLVEILVSSEFDPNARLTHYQSSCIDLICNILPKVITASARCWRIVARVELWRRRPWEALECHERAYRAVMHNPELESNESVWNEVVDACADLVSAYESLGELPGRHGADDLVCKDWKYKARTTVRSLMSKGKDMWEDSPGWDRLQEMKQDLSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM89</accession>
    <name>G3AM89_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcriptional regulatory protein DEP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_137097</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374902.1">
        <property type="nucleotide sequence ID" value="XM_007374840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM89"/>
    <dbReference type="FunCoup" id="G3AM89">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="GeneID" id="18870014"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137097"/>
    <dbReference type="eggNOG" id="ENOG502S36P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031124_0_0_1"/>
    <dbReference type="InParanoid" id="G3AM89"/>
    <dbReference type="OMA" id="DTPWANP"/>
    <dbReference type="OrthoDB" id="20886at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013907">
        <property type="entry name" value="Sds3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21964">
        <property type="entry name" value="BREAST CANCER METASTASIS-SUPPRESSOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08598">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01401">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="91"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="130"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="153"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="471" mass="53203" checksum="5D43129762CC8D61" modified="2011-11-16" version="1">MDFLDDDDEAKDRSQGEVDVSDLRRLSKLTELARLKDVDSSDESEKETSIKDEVDEGNQEEETQDENSNSNNIERKRELEDEETSNNNKKSKVEKATGKKDRKQQALELNDETEDGKIKDEPTITNEIVIPDDEQVSVDAVIENDDITSNADAGDEANEAEDEDDEASSKNEDNQEDETSHKEGDDELEADEADDDDDDDDNNNSNTNEQEQLEDQVDLNEQRKLAIEELISIESRFAELRDKLYQDKLNLLERELTLCLEGSHPELSKVYYKINGFYQDSLKLANSNLAYKLKCIDKETIATRTSIHQNFLKNLMDTKNQMITDTTSLWYKINKERNQLDQLVPAFNFAAMPDVSQASAPIEYDGGSGSFVAANAAQYNGSVYHDEALIPISKRAVKQNTLVELVHQRNSINEQLGVLNGLIQFHGFPVAVNSTLSEQESNPKTYPEELLLKRATDNEINDDLKAMGIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMA5</accession>
    <name>G3AMA5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60763</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374918.1">
        <property type="nucleotide sequence ID" value="XM_007374856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMA5"/>
    <dbReference type="STRING" id="619300.G3AMA5"/>
    <dbReference type="GeneID" id="18873553"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60763"/>
    <dbReference type="eggNOG" id="ENOG502S3T7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1111727_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMA5"/>
    <dbReference type="OMA" id="SEWNFIL"/>
    <dbReference type="OrthoDB" id="2147978at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="248" mass="29405" checksum="FBA745C2E20C07A5" modified="2011-11-16" version="1">MKFTFKFPLAQALKFKPGKFNFQLEQEIRTKLDSLPSVTKIYTNPDGTPRVPTDEEFKTIAELQNLYYKRNHSEWNFILGTVPKDQLAIYKDNSQDLTNFQFVTKDAGKIIDKIPYKDDTTGELKWKVIRETEKAEGWEFPYFYIVLPIFAYILYVKYQNQMIVREQDGPEWAERELRLRAMEDYFHGDTEKAKKFLSNEGKSAREIRDRDDLVVARILAGDYDKLAQLKKNMPKDFRRFEEDPVLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AME3</accession>
    <name>G3AME3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_136800</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374310.1">
        <property type="nucleotide sequence ID" value="XM_007374248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AME3"/>
    <dbReference type="FunCoup" id="G3AME3">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AME3"/>
    <dbReference type="GeneID" id="18870000"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136800"/>
    <dbReference type="eggNOG" id="KOG2102">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012624_0_0_1"/>
    <dbReference type="InParanoid" id="G3AME3"/>
    <dbReference type="OMA" id="VFCIDSE"/>
    <dbReference type="OrthoDB" id="2285229at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF9">
        <property type="entry name" value="DIS3-LIKE EXONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNB" evidence="1">
        <location>
            <begin position="544"/>
            <end position="900"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1030" mass="116795" checksum="19CD38B89988B0AC" modified="2011-11-16" version="1">MKLINEKSTKFLGKYVDPTSKLDLRLSSKKILSEIEEAFIKRGNDRFVTPSKAWKENVLSGEESKGISEQISSCLSNSKVNGACIVQPPKVGDIVTLSDNLRLYIIAAGPKSIKSGSYTFINARGEIVFGKSGMIRDRVPGAIPQRFHDYLGNCVSLEQKFDGVAPVGMAESLTRSAPETIKTKDEKMNDLEEPEVNDPTDFVVSQATSQLLVDTDINTYIVPLLARSTYAGALSQLCIDSFKKVSSINQKLERLHRTLQYDADGNLNVPRTLTIFEILYHLEQNPKNSIQSKQNGNSKLGVSLDFSQNYEDYDYSASTYLATLFALRKQSRLWNLEATSQNECQTRVTVLPVSDVSYIDRVMKYLKEGGIDEFSKYCAGILSGEKPTKQPPLYIDIIGMLKDFVNRKFDHDPAVETVLVYVLRGIEKHMLPSNLVFETSNYVNEYSRTRAYDLLVELEKNDSIVNPFKWSKTSSWPNEKTSYVSDLSQSYYDCLDDNATTSSENTGLDQLDQQQLSNLNEPIDSTQMGYSNDFFDSDPMNDIREDLTNVSVYCIDSEFAHEIDDGISIHEDEGKYVISVHIAEPSSYVKPDSTLSSIAFNRTSTQYFPETALLMFPPIISNLSGLGIDSQDTRAFSIQYKLDKGLLDEFFKQRLTDPESSIDSTLLNTVKSQIEQTKEIKYSLLRNFRQGFTYKKVAKVLRSENRQELYRTDQDYNNLFKLFQLSTILNGIRSQNIPVEMTQNNSNLQVLETGDDIASESRYIEKGEGHEIRVKNSRQSIIIPKELDIEDSVSLVTENMIFANYLTGAIAKEKNIKILYRTFEFNISDELKKQFQELRSGNSIRKDEFDFGSFVSLAKYTVSHGTHALVGLGSYTTITSPMRRYVDMINQWKFQDYFLGREEGFIKDSNLENIANIVNSKAAYAKKPSAYAQKFWTGIFLREYKRLFEAGEIKNPIEFSLVLKAAPRKDAVIGVTCEKFPNMIANLEVSDALMEDYKNGLIGKDGRISSNRLQIARIDYVEDDLLFKYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMF1</accession>
    <name>G3AMF1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_60147</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Transport of phosphate groups from the cytosol to the mitochondrial matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374317.1">
        <property type="nucleotide sequence ID" value="XM_007374255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMF1"/>
    <dbReference type="FunCoup" id="G3AMF1">
        <property type="interactions" value="814"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMF1"/>
    <dbReference type="GeneID" id="18873242"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60147"/>
    <dbReference type="eggNOG" id="KOG0767">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039456_2_0_1"/>
    <dbReference type="InParanoid" id="G3AMF1"/>
    <dbReference type="OMA" id="TFREAVP"/>
    <dbReference type="OrthoDB" id="427452at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000076">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000131">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 14" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="46"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="142"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="241"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW32802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="334" mass="36085" checksum="B49709C11A116CC3" modified="2011-11-16" version="1">MAGNLADAITQPVNDQLKKIAEKSTGGSGSSGSANPPHKIQLFTPEYYAACTLGGITACGPTHAAVTPLDLVKCRRQVDPHLYKSNLQGWKSILKTKGDSILTGFGATFVGYSFQGAGKYGFYEFFKKTYSDLVGPKFANDYKTGVYLAASASAEFLADIALCPWETIKVRTQTTIPPYAKSLTEGWSKIVAADGYGGLYKGLVPLWGRQIPYTMVKFASFENVVAAIYSYWGKSSYTNLEKTGVSFLAGYIAGIFCAIVSHPADVMVSKVTSERKAGEAIGETLGRIYKRIGFVGLWNGLPVRIVMIGTLTGFQWLIYDSFKVYVGLPTTGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMF3</accession>
    <name>G3AMF3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_60150</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374320.1">
        <property type="nucleotide sequence ID" value="XM_007374258.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMF3"/>
    <dbReference type="FunCoup" id="G3AMF3">
        <property type="interactions" value="469"/>
    </dbReference>
    <dbReference type="GeneID" id="18873244"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60150"/>
    <dbReference type="eggNOG" id="KOG0764">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_6_1_1"/>
    <dbReference type="InParanoid" id="G3AMF3"/>
    <dbReference type="OMA" id="CLQYADH"/>
    <dbReference type="OrthoDB" id="10266426at2759"/>
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        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
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    </evidence>
    <sequence length="374" mass="41469" checksum="CE606496E2470A89" modified="2011-11-16" version="1">MTRIDRTILNEGIAPSPVGAVPVPLLREYDTEPTQATTFTAAPPVLLQTTPSPIFSKFSPNQLITMAGAASGFLAGVAVCPLDVVKTRLQAQGDAIAYMPSKYNGFWHTLKTILAEEGVRGLYRGLVPITIGYLPTWTIYFTIYEKGKKVFPSILHKYLNVNNDDATHFFSALTAGLTSSIVVNPIWVVKTRLMIQTGKKSTIYGSSKTVGRTYYKGTIDAFVKMYREEGIRVFYSGLLPSIFGLLHVGIHFPMYEKLKNILHCNMSEGNDSRGMLARLIVASSVSKMIASTITYPHEILRTRMQIKNHGIQPVKHVLVRSTIDIWKKEGWKGFYAGYGTNLARTVPSSAVTLVSFEYFKTYLLRVSGNGPVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMQ9</accession>
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            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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    </dbReference>
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            <end position="212"/>
        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="533" mass="58803" checksum="26481E0E916B252F" modified="2011-11-16" version="1">MAYSLDANKWKAYQFSDPFAVGPDCDAYPITNLRQEIKFTDTASDALKLGYIACDFCDPCHSSIVDVDLLIKCVSTINHQIGFMPPLLDEDEEYNTQKIKENILETKRANEEQILKTFNNVISNEGSYQHRYSVPIFNTSGKLSKDLESTPLSKNDSDHYRLVDLACRHLALAAAVSVFQPHSRIVTSPEVEDRNPSTGGSKKKRRRRRRGGVLGFKELAAKSKLSAWHFHRVFKSVTGLTPKTYGDKCFDFLKKVEKSGEYTTFQEAPVYNSPPMSSNSQSRPVSSIKDGESSLGASPLQQNSPYRMVKLENTPMLFKTDFFSPTQQLSQQTYTNPSVMITPPNESIQYTTLSPTQALDSQGSATSIPNLNISAELQRQLNPMTQTSSSTTAYGDLPATSADDIFPSRAFSLPELSRSGTSALLSNTFPADLNVGVAAAQQSLLDPQVTHQQGFDPIAPEYDLSREIYPELSRSLGPSHANVTFEIGPSDLASNAMVGTLSAPENLGYSTTEDIFSNDQKYNPQMLSTNIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN08</accession>
    <name>G3AN08_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">High-affinity methionine permease</fullName>
        </submittedName>
    </protein>
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        <name evidence="7" type="ORF">SPAPADRAFT_61496</name>
    </gene>
    <organism evidence="8">
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        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
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                <person name="Dale B.E."/>
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                <person name="Zinkel R."/>
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            </authorList>
            <dbReference type="PubMed" id="21788494"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000102">
        <property type="term" value="F:L-methionine secondary active transmembrane transporter activity"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042883">
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="High-affinity methionine permease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="325"/>
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        </location>
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            <begin position="370"/>
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        </location>
    </feature>
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            <begin position="403"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="436"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="472"/>
            <end position="496"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
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        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EGW32422.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="546" mass="59925" checksum="1E1A4D4BF6452895" modified="2011-11-16" version="1">MALSDIYKNSSEKHPEQDTDAHSLSTGSEQNVGVVQVDKNVKEIGVISAAFLMLNRMLGAGVFSTGSTIFALSGSVGTSLMMWFAGTIIALSGLLVYMELGSAIPRNGGEKNYLEYMFKKPKFLVTAMYASYVFFLGWAAGNSIVVGEYILNAAGKEVTQWNSRGIGIAVITFAFLINAIHIKTGLFLANALGVFKVFIVLFITVTGWVALGGGIKSNNFQPTHNFTDAFKGTTPTAFGIVNALYNVIWSYVGYSNANYALGEIKNPHKVLRLAAPIAFVSLGIIYMFVNIAYFAVVPKEEIASSERILAAAFFRYAFGNKAEKAASVFVALSAWANVMSVIFSQGRIVQQLGREGSLPFSRFFATSRPFGTPFVGLMQHWIVCIVTIVAPPPGDAYNFILNLISYPLNVVNTVVAAGLLYIYYKKSRGLTEWNPPIKASLPVVFFFFLASLYLIVAPYIPPVDGQTVYNSLPYWIHAVVTWGVFGIGLVYWLVWVKILPYFGSYKLVAVEVLGDDGFWRNKIYKVSKDSVESEIHDAELDKAEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN34</accession>
    <name>G3AN34_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD synthase</fullName>
            <ecNumber evidence="2">2.7.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">FAD pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">FMN adenylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_153392</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>FMN + ATP + H(+) = FAD + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.2" evidence="2"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375724.1">
        <property type="nucleotide sequence ID" value="XM_007375662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN34"/>
    <dbReference type="FunCoup" id="G3AN34">
        <property type="interactions" value="160"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN34"/>
    <dbReference type="GeneID" id="18870954"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_153392"/>
    <dbReference type="eggNOG" id="KOG2644">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056971_3_0_1"/>
    <dbReference type="InParanoid" id="G3AN34"/>
    <dbReference type="OMA" id="EEFVQWS"/>
    <dbReference type="OrthoDB" id="270728at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23948">
        <property type="entry name" value="FAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000187">
        <property type="entry name" value="Probable FAD synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293:SF9">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293">
        <property type="entry name" value="FAD SYNTHETASE-RELATED FMN ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="13">
        <location>
            <begin position="77"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="13">
        <location>
            <begin position="171"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW32448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="279" mass="32655" checksum="20C36C68EFE9E232" modified="2011-11-16" version="1">MTTQDKRDIQHGFLQRCEQAYDLVQGFLHDTLPWSPVPTTKHGFKYNHVLRQQVKDKVLLSMDKLEMSIDLHGLEEIAISYNGGKDCLVMLILLLATIHKKFASSNFENPSYNMLPRDYKLDSIFINAEPQFPDLLQFIEQSTDYYHLNPIIIKSSLKDGFEYYLNNMNTKVKSVIVGIRYSDPYGSTLHYEQPTDHDWPKFLRIHPILHWNYVDIWDFLIGCDLDYCKMYDWGYTSLGGINATVPNPYLKIEGTDKYLPAYMLTEHADERERLGRNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN97</accession>
    <name>G3AN97_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61546</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375756.1">
        <property type="nucleotide sequence ID" value="XM_007375694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN97"/>
    <dbReference type="GeneID" id="18873945"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61546"/>
    <dbReference type="eggNOG" id="ENOG502REVP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1332668_0_0_1"/>
    <dbReference type="InParanoid" id="G3AN97"/>
    <dbReference type="OrthoDB" id="4020218at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="206" mass="24072" checksum="2385C5CB9176447B" modified="2011-11-16" version="1">MFSKKNKSVIDKVQLFLIISIKLFMTGLKLIVPLTKYVYHKFQHNQFYLLNSKNADKLIDYILKLMNYLDTKLNNNEDIIDKISQHDYIKSEEQMEQIYKDFTNYAGELFDPSKVMDKYISKDDSIKKGMVEFFINRMSREDKSSYEKNPKYNMYYSGRSKNPTPRSTTPKPNTSRNSHLFDRRGNSTSGSETSSVSGYKSCASYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANB7</accession>
    <name>G3ANB7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_61563</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ELP4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375776.1">
        <property type="nucleotide sequence ID" value="XM_007375714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANB7"/>
    <dbReference type="FunCoup" id="G3ANB7">
        <property type="interactions" value="858"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANB7"/>
    <dbReference type="GeneID" id="18873957"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61563"/>
    <dbReference type="eggNOG" id="KOG3949">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040685_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANB7"/>
    <dbReference type="OMA" id="QGMLKVH"/>
    <dbReference type="OrthoDB" id="289162at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19494">
        <property type="entry name" value="Elp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007573"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00020265"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="436" mass="47683" checksum="A2A220BEA87F105F" modified="2011-11-16" version="1">MSFRKRGEIIGGNPNRVPGATSNAIPGRTPVPVIPGRENPLMGRSAGITPTRSPITRASPATQDTTNLIMKNPGVRPSVITSQPTISTGTADLDKILLHQGLPIGHSLLVEESGTTDFASVLIRAFASQGIMHNRITNDVNCHVIVVGLSPQWSSDLPGLYKGSSREQKKARIKEQESKVSVTNLAQASSGPNVRAAAPDLKIAWRYGLNKKPEEESNGSDSTATYEHYNHQFDLTQKLTPGPNAQDISYIPIVNNYAQIVQQVSAIVKNQVKSNPGKILRVVIPGILNPSIYPPATASPTFIFPLVHALRGLLRQYSSNLSVIASLALDLYPRDSNVTGLFENVFDSVIHLQPFNQEMSQLIEKAYKNEPSKIQQGLVNIIKVPVLSEKGLMMIHDGEYAFKNGRKKFEIEEWGIPVEDDGKDEKDAQTTKNIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANC1</accession>
    <name>G3ANC1_SPAPN</name>
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            <fullName evidence="3">Trafficking protein particle complex III-specific subunit 85</fullName>
        </recommendedName>
    </protein>
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        <name evidence="1" type="ORF">SPAPADRAFT_61564</name>
    </gene>
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        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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            <taxon>Eukaryota</taxon>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="TRAPP_III_complex_Trs85"/>
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        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12975">
        <property type="entry name" value="TRANSPORT PROTEIN TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12739">
        <property type="entry name" value="TRAPPC-Trs85"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="430" mass="50668" checksum="9E59488A7C011DF9" modified="2011-11-16" version="1">MFGHMVGNDDLLLPFETFNHPISQVFVISMQDSIDELRNMIVKFRNHNYPLYFQMNDLLFHVVILYQEDSNDVISFQNEIKSKLNLNSTVIPIETTVDTTIRYKLNEFIVKNLIPHIQMKIRLWDDQYLQPKKSITNRFISVSKRFFNNNSDSQLASSTSTASTASPFNYNENYYYKGSSQQVLRKLADWSLMINDFKYAYTIYDLIKKDYSQDKAWIYVASTQEMCIVSLLLAQTDQSSISGPDKNTLRKIRHDIIEPFMDNLTYTYKSRFNYTHLNRKCLIIVIELLLCMNKLYNLSYWWIDLIELYLLQLVDDIEHETQGQHSIIKALFYERLGYSFGHYYIPPIVDKSEEENEVDEEEDVNPNKLVPPVNNSVIGLTRFRKSSLWYLLSIREWLGILDKSKLTFVIDNINVEYQVHDSVIKEILQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP56</accession>
    <name>G3AP56_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Deoxycytidylate deaminase</fullName>
            <ecNumber evidence="7">3.5.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">dCMP deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_51163</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.12" evidence="7"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375903.1">
        <property type="nucleotide sequence ID" value="XM_007375841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP56"/>
    <dbReference type="FunCoup" id="G3AP56">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP56"/>
    <dbReference type="GeneID" id="18871527"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51163"/>
    <dbReference type="eggNOG" id="KOG3127">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047993_0_1_1"/>
    <dbReference type="InParanoid" id="G3AP56"/>
    <dbReference type="OMA" id="YFMRLAD"/>
    <dbReference type="OrthoDB" id="6710946at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004132">
        <property type="term" value="F:dCMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01286">
        <property type="entry name" value="deoxycytidylate_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000035">
        <property type="entry name" value="dCMP deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015517">
        <property type="entry name" value="dCMP_deaminase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035105">
        <property type="entry name" value="Deoxycytidylate_deaminase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11086:SF18">
        <property type="entry name" value="DEOXYCYTIDYLATE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11086">
        <property type="entry name" value="DEOXYCYTIDYLATE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="10">
        <location>
            <begin position="206"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="356" mass="40048" checksum="F91BF66E23F8BBE9" modified="2011-11-16" version="1">MLIGISGTLSSGKTEVARYLTFQGFKLISLSKPGDPITSLVDSSDSLPESDSDTLNEKLDTLVISNGNVSSYYSKMEVHKKFDDLEDLLDFVTVNWRENYVLSHMNDMQMLVSLQKRPFFLHISIDAPIGLRYQRYLLKVASHASRLSLEDFVVKNDELLFNVDNPLIEINNQARIKIINTSASIKDLYIKLSELNLLDFSRLRPTWDSYFMRLADLAALRSNCMKRRVGCVIVRDYRVIATGYNGTPRHLTNCNEGGCPRCNKGHGSGASLSTCLCLHAEENALLEAGRDRIRGDSVLYCNTCPCLTCSIKIVQSGIKEVVYAQNYSMDSYSHKVMSEANIILRQYQPPVDGIFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>G3APB9</accession>
    <name>G3APB9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_61175</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NFYC/HAP5 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375372.1">
        <property type="nucleotide sequence ID" value="XM_007375310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APB9"/>
    <dbReference type="STRING" id="619300.G3APB9"/>
    <dbReference type="GeneID" id="18873760"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61175"/>
    <dbReference type="eggNOG" id="KOG1657">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045277_1_1_1"/>
    <dbReference type="InParanoid" id="G3APB9"/>
    <dbReference type="OrthoDB" id="1272441at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22908">
        <property type="entry name" value="HFD_NFYC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000017">
        <property type="entry name" value="Ccaat-binding factor complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF8">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="9">
        <location>
            <begin position="98"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="186"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="313" mass="35707" checksum="BBFB8B785BC37A20" modified="2011-11-16" version="1">MEGYENTYLDNEDLVHYQQQQQQPQHHQYMEELEQEEEPASHPIVDEAELLAAQAAAEAAAAAQQPMDAFNNVAQGLTGKYRDMMMQYWQETINSIEHDEHDFKNHQLPLARIKKVMKTDEDVRMISAEAPILFAKGCDVFITELTMRAWIHAEENKRRTLQKSDIAAALTKSDMFDFLIDVVPREEEKPKKSYPSSRTNGYNTHPAATSSSSQQQEQLPPQHLPPPTVESDIVHEQQMLQQQQQEQQNEHLQSQPVSHHASHHSSHQPTPQPTLESISQAPVPANGGSTHQVEESRDGHYPNFKVDSYQANY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3APC9</accession>
    <name>G3APC9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_50696</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375383.1">
        <property type="nucleotide sequence ID" value="XM_007375321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APC9"/>
    <dbReference type="FunCoup" id="G3APC9">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="GeneID" id="18871475"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50696"/>
    <dbReference type="eggNOG" id="ENOG502QRFY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096561_1_0_1"/>
    <dbReference type="InParanoid" id="G3APC9"/>
    <dbReference type="OMA" id="ADWMTKH"/>
    <dbReference type="OrthoDB" id="289247at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040250">
        <property type="entry name" value="Nucleobindin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19237">
        <property type="entry name" value="NUCLEOBINDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19237:SF20">
        <property type="entry name" value="NUCLEOBINDIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012655289" description="EF-hand domain-containing protein" evidence="3">
        <location>
            <begin position="16"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="102"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="222" mass="25408" checksum="92B346B5C4D9C5B3" modified="2011-11-16" version="1">MKLSLFLPFLVLVNAHGDHSGHGSDKPAVKPPQLTWAQWHMAQEHNLDDYDAATFFTLHDLSGSRAWSRADILNIYGLINPRVGDGSGMGSHGNNEEISKQAKDKVVNTILGLIDSNKDGQISWDEWIQFSAKGGELPDLGYGPGHHLDFESEYEEHHWKKYHMNDDPDVLIKHKEDIEHELLHHEHEIEETHHKAPQIRQVTKNFLSKINLHNLTPRYTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3APH5</accession>
    <name>G3APH5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61230</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375422.1">
        <property type="nucleotide sequence ID" value="XM_007375360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APH5"/>
    <dbReference type="GeneID" id="18873787"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61230"/>
    <dbReference type="HOGENOM" id="CLU_2484747_0_0_1"/>
    <dbReference type="InParanoid" id="G3APH5"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="87" mass="9508" checksum="C757A061253FB75F" modified="2011-11-16" version="1">MDVLRSIYFVVDLGDKEVSLAQSDFSQGNQDIEVFPPPGVNRTLASRGRSEHQESSDTPEEKASSANSIVSSIDVLLCLAILLTWLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3APH6</accession>
    <name>G3APH6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">General amino acid permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_61231</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375423.1">
        <property type="nucleotide sequence ID" value="XM_007375361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APH6"/>
    <dbReference type="GeneID" id="18873788"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61231"/>
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    </dbReference>
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    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000017">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF1">
        <property type="entry name" value="GENERAL AMINO-ACID PERMEASE GAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="359"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="430"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="477"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="510"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="10">
        <location>
            <begin position="84"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="584" mass="64373" checksum="284A0AB5C4788447" modified="2011-11-16" version="1">MSDIKHNTDSITESKSKIESSSSLNSNTHELTYANQSKPGIWREFADSFKRAEDVVIIRDLENGDKEEIRQAPVLKQTLKKRQLQMIALGGCVGSGLLVVSGQALKVGGPASLMIAWAIVSSFLYCTMQSLAELSSAYPVSGSFATYAAKFVDPSWGFAMGWNYALFWVVVLPLELVAASMTIKFWNSSINSVVWIAIFYVLIIGLNLYGNKGFGETEFIASIIKLLGVVGFNILAVVLICGGGDQGYIGGKNWHPPFQTGFKGVVSVLLSATYSLAGTELVGLTTSESEGNPRKVLPKAIKQVLWRIMIFYMLTLMLIAFLVPANSPQLLDAQGSSDASASPFVIAIRAGGIKVLPSIFNVVILVALLSIGNSAVYGFSRTILALGEQGLAPKIYTYVDRKGRPLAGICTSAIVGLLSFVSASKYEDQVFGWLVSLSGLSTLFTWGSINLSFIRFRSGMRAQDRSLHELPYRSNTGVWGAYYGLIMNFVVLALQFWLALFPIGQKPSPVLFFQTYLGAIIIVVFYFGHKLFARNWILIIRAKDIDVDGGKRETDIDLLRQEIEEEKEHLKSQPLYYRMYQFWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3APJ2</accession>
    <name>G3APJ2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_139723</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="18870231"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139723"/>
    <dbReference type="eggNOG" id="KOG0027">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="InParanoid" id="G3APJ2"/>
    <dbReference type="OMA" id="EQMDGTQ"/>
    <dbReference type="OrthoDB" id="429467at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050403">
        <property type="entry name" value="Myosin_RLC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23049">
        <property type="entry name" value="MYOSIN REGULATORY LIGHT CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="18206" checksum="C2C2FBD088B18012" modified="2011-11-16" version="1">MSHLNSLTSTQKTQLRNAFALIDGETKDSVITKDDLRKLYSTLGLPPPTEDQLNSMLGDEDKGINFTQFSNIMAKELSKFEDRAVIYEALKTFTNDEKSTGLTIDLQEFKEACCSVQLGEIGSGDHRLSRSTFDRLVQGFVQEQVNGKQVFLATKWLDAYID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>G3APS6</accession>
    <name>G3APS6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_138860</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375523.1">
        <property type="nucleotide sequence ID" value="XM_007375461.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APS6"/>
    <dbReference type="GeneID" id="18870157"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138860"/>
    <dbReference type="HOGENOM" id="CLU_2277091_0_0_1"/>
    <dbReference type="InParanoid" id="G3APS6"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="126" mass="13333" checksum="5BEC26FE7D206329" modified="2011-11-16" version="1">MVANTADAVKAHAITIPAGPPVLNAPPEPTNSPVPMDDPIAIICKCLDNNFLLKLEFAEFKLILSRSLSSLSVPSAWACVNVCVGIAGLNESIKELHTPVFSCGGVSKRLISSIDEKSCLSELIFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3APU0</accession>
    <name>G3APU0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Regulatory protein MIG1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_61343</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in glucose repression of glucose metabolism genes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the creA/MIG C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375537.1">
        <property type="nucleotide sequence ID" value="XM_007375475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APU0"/>
    <dbReference type="FunCoup" id="G3APU0">
        <property type="interactions" value="1235"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APU0"/>
    <dbReference type="GeneID" id="18873851"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61343"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027078_0_0_1"/>
    <dbReference type="InParanoid" id="G3APU0"/>
    <dbReference type="OMA" id="NKDDRPY"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000433">
        <property type="term" value="P:carbon catabolite repression of transcription from RNA polymerase II promoter by glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000089">
        <property type="entry name" value="DNA-binding protein creA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000152">
        <property type="entry name" value="DNA-binding protein creA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051007">
        <property type="entry name" value="creA/MIG_C2H2-ZnF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47428">
        <property type="entry name" value="REGULATORY PROTEIN MIG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47428:SF1">
        <property type="entry name" value="REGULATORY PROTEIN MIG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="43"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="343"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="244"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="343"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW32261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="457" mass="50779" checksum="0E28C70BB75D51EE" modified="2011-11-16" version="1">MMQTKEKKNKEDRPYKCTFCEKAFHRLEHQTRHIRTHTGEKPHACTFTGCTKRFSRSDELTRHLRIHNNPTNRKRKNKNEVVNVVEPIPIGYQQQPNGYPVYFVPQPNGYMQPVVPQPQPGQMMPIQVQPIHMQPQQLQQPQISRPQSAQAIPIPNHYKQQGTAVFSLPSSPTNFVQPQQPYPQQQVQQHLASTRPMNMSRTTSSEGLKLPPLNHSPPTRLISVESNSSIGSINNSSGVFSQASSTSHSLGTSPETAYPPTVGATTPSFTNLNDYFSSKNTRIFNASSTSLSSLTSKLRVSNSSNNLTALPRMITPLNKSSSQSSKITKQQSSTSLNLEFFQQSQFRKSRPNSPTMGSKRQVPTGPTTQQAPFVISPNDTPLQTPIQSPHLRPASAAPPSTGFDRLLIAEATEKLASIANKGTTLPPIRSVLSFTSLKDYPEPVVQDKKMNLNNILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3APZ7</accession>
    <name>G3APZ7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">P/Homo B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_139715</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S8 family. Furin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375594.1">
        <property type="nucleotide sequence ID" value="XM_007375532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APZ7"/>
    <dbReference type="FunCoup" id="G3APZ7">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APZ7"/>
    <dbReference type="MEROPS" id="S08.070"/>
    <dbReference type="GeneID" id="18870229"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139715"/>
    <dbReference type="eggNOG" id="KOG3525">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002976_2_1_1"/>
    <dbReference type="InParanoid" id="G3APZ7"/>
    <dbReference type="OMA" id="NEDNGAY"/>
    <dbReference type="OrthoDB" id="300641at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007323">
        <property type="term" value="P:peptide pheromone maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000026">
        <property type="entry name" value="proprotein convertase subtilisin/kexin type 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000005">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF14">
        <property type="entry name" value="NEUROENDOCRINE CONVERTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 14" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442536" description="P/Homo B domain-containing protein" evidence="17">
        <location>
            <begin position="19"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="776"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="domain" description="P/Homo B" evidence="18">
        <location>
            <begin position="497"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="651"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="659"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13 14">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13 14">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13 14">
        <location>
            <position position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW32318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="796" mass="87701" checksum="4A15765E278FB07C" modified="2011-11-16" version="1">MLVFSLLAAVSLIALSFQKQLPPRDYENNNYFLVELDTTTSQKPLIEFIEKYKSHYTFDHQLPLDNYYVFKINKSHPHNTFLGNLNSEHSTEDVQPLMKRDVGFEDVYDDFMSNDALKSIHLLPPKRLVKRESVPVDTHDIESSSEAKNRPQIELVKQKLEEVTKRLEIKDPMFERQWHLINTFHPGNDINVADIWLEGKFGKGVVIAVVDDGLDITSDDLHDNFNSEGSWDFNDNSDLPLPRHPDDYHGTRCAGEVAAVKNDFCGLGVAFKSQVSGIRILSGRLTNADEASAMVYGLDTNDIYSCSWGPIDDGKTLSEPHPVVKKAMLKGIIEGRDSKGALYVFASGNGGRYGDSCNFDGYANSIYTITVGAIDFQDQYAPYSETCSALMVVTYSSGSGEHIHTTDLNKKCTEADHGGTSAAAPLASGIYALALSVNPELTWRDIQYINVLSAVPVNEDDGIYQITSIGRKYSHQYGYGKLDATKLVHLAETWKNVKPQAWYYSDVIEVQKAVTSLNEVIKSSVVVTEEDLKLMNVARVEHITVKTNIRATYRGKVGMKLTSPHGVVSYLATYRPLDASMSGFLDWQFMSVAHWGESGVGEWKVEVYGETSITFVDWQLRIFGESIDASKATKFDLKADYAEMRRKLKNEQVSAMSETPSSVLSSTLKSSTSTMSQSVSPTSTSTSMPVTAPKYHSSSASMISTTSTFSKLPITILSKSSIFPTSTRSANSSRSSISSRFSTLSVLSSLSVSSTLILSSAISAPSASSVLSSSSSVLSSISFSFAAFFIIFILIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQA4</accession>
    <name>G3AQA4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_154622</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376229.1">
        <property type="nucleotide sequence ID" value="XM_007376167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQA4"/>
    <dbReference type="FunCoup" id="G3AQA4">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQA4"/>
    <dbReference type="GeneID" id="18870992"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_154622"/>
    <dbReference type="eggNOG" id="KOG1505">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059043_1_0_1"/>
    <dbReference type="InParanoid" id="G3AQA4"/>
    <dbReference type="OMA" id="RSKFSEW"/>
    <dbReference type="OrthoDB" id="189226at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036149">
        <property type="term" value="P:phosphatidylinositol acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983:SF70">
        <property type="entry name" value="PROTEIN MUM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="214" mass="25235" checksum="79E0CEEE65722675" modified="2011-11-16" version="1">MASSTSEITLPVVNFFSWFLVWRVPTIKILLHMLKCDENWEIDQKSVDFVFSRLLKSKNSQWIVLFPEVNIWTREGAGLQRQLSEKYFLPQFTHLLYPRFSGFFNVITALADIKPQPYSNLYDITILYVREDSQQISFRPPTLLEFFSSKVPITVTVNVKIRSVNRVPQKRHKLERYLEHLWRNKDKLIAQLKVENDVEVINSSLKCINDVNDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR24</accession>
    <name>G3AR24_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_62293</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sphingosine N-acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376463.1">
        <property type="nucleotide sequence ID" value="XM_007376401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR24"/>
    <dbReference type="STRING" id="619300.G3AR24"/>
    <dbReference type="GeneID" id="18874252"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62293"/>
    <dbReference type="eggNOG" id="KOG1607">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028277_2_2_1"/>
    <dbReference type="InParanoid" id="G3AR24"/>
    <dbReference type="OMA" id="KLYCLLQ"/>
    <dbReference type="OrthoDB" id="537032at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050291">
        <property type="term" value="F:sphingosine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016439">
        <property type="entry name" value="Lag1/Lac1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560:SF0">
        <property type="entry name" value="LD18904P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560">
        <property type="entry name" value="LONGEVITY ASSURANCE FACTOR 1 LAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005225">
        <property type="entry name" value="LAG1_LAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="9">
        <location>
            <begin position="139"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="358"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="361"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="423" mass="49144" checksum="D3FB915B100E95FF" modified="2011-11-16" version="1">MSAVQPDRKIDSTTLSRDTNSSTSSTNSFTAEDRKYHASFLASIERNQIRLSRSIIIVLTALHFSGIPSITKYTSKFFHLQNQIGVANNRPIYDIHIDDTYYVIFWVIAVTFLRSFLMQWCFSPFAKHFCSITSRKAKIRFAEQSWSFVYYTVSLICGCYLYYNSPYFNNADQIFVGWPNHTLHASLKRYYLISTGFWLQQIFVLNIEQHRKDHYQMFSHHIITCCLIIGSYYYYFFRIGHLILMTMDSVDILLSGAKLLKYANYSTACDVMFILFMVGWLVTRHGIYNYLFYHTWHNARRLMAESICDPTKIQKRCWTPTIINVFLGLLGGLQILIIIWMYFICKVAYKVITGSGAEDVRSDEDDTDAEEVDEIEPESAFEDETDETDEPIGIKSVGMDAFSSSSDSTLDDESDRRRRLRGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR65</accession>
    <name>G3AR65_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">candidapepsin</fullName>
            <ecNumber evidence="4">3.4.23.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_51269</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential cleavage at the carboxyl of hydrophobic amino acids, but fails to cleave 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17 and 24-Phe-|-Phe-25 of insulin B chain. Activates trypsinogen, and degrades keratin.</text>
            <dbReference type="EC" id="3.4.23.24"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.24" evidence="4"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376018.1">
        <property type="nucleotide sequence ID" value="XM_007375956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR65"/>
    <dbReference type="GeneID" id="18871540"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51269"/>
    <dbReference type="eggNOG" id="KOG1339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013253_9_1_1"/>
    <dbReference type="InParanoid" id="G3AR65"/>
    <dbReference type="OMA" id="MAFATNA"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05474">
        <property type="entry name" value="SAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033876">
        <property type="entry name" value="SAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 13" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase A1" evidence="14">
        <location>
            <begin position="74"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW31240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="402" mass="44451" checksum="C1CD157A5E379DA9" modified="2011-11-16" version="1">MRKKMNLMQVGIRISISIVLVLLVQAVFIDRPEFRKIFGEDTLVRNVLEAIHPKRDDSKYLELTLKLSADRSLYTAAIQLGSNKDEVNLLVDTGSADTLVISSDAKCYIDNRVYNAFNSCFDMGTYDLTRSTTASGRIQDFNARFFDGHYSSGMVIKDAVHLSDSKVIDGFEFGIVNSTTTSNGILGISRTTQSNVASGYSNFPCALKKAGLINKLAYSIFLNNSDEGNILFGAIDKAKYYGKLVFLPFASSKFSSVTVTSINFGNGNSVKVNQAFLIDSGTTFINVNAAIYNDILNNFEGERTSNNKLVVDCNQPADRFYTFKFPGVSIKVPFTDLVTRAYDSTTGQFMDDCLLAVTQNPTMLILGANFMRHAYLAFDLERKRIGIAQANYTSESNIVSFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR92</accession>
    <name>G3AR92_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TEP-1 second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_56147</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376045.1">
        <property type="nucleotide sequence ID" value="XM_007375983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR92"/>
    <dbReference type="STRING" id="619300.G3AR92"/>
    <dbReference type="GeneID" id="18872006"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56147"/>
    <dbReference type="eggNOG" id="KOG0265">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_2_1"/>
    <dbReference type="InParanoid" id="G3AR92"/>
    <dbReference type="OMA" id="ILYMLPG"/>
    <dbReference type="OrthoDB" id="1068471at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056829">
        <property type="entry name" value="Beta-prop_TEP1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052234">
        <property type="entry name" value="U5_snRNP_Component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006:SF1">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25047">
        <property type="entry name" value="Beta-prop_TEP1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="TEP-1 second beta-propeller" evidence="7">
        <location>
            <begin position="17"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="26"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="73"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="115"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="293"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="326" mass="35802" checksum="091555667DBA565B" modified="2011-11-16" version="1">MSVAKRQRRSIDDTNAPNIQESTSLENGHQGTVYCTKFNHEGTKIASGGHDKTIQLWNLPTSTEDPDPNYLELLGHKGSVISVSWLFDDGTLVSGSADSTIGFWDVFTGRRLRKCTGHEGVINQVTTCSDHVVSVGDDGKLLFWDIRFKDPVANVQTKYPLLSCVFNHKGNTVYTSGIDPAITAYDVRVLNKPLWKEENGNHSSVTSLAISKDDTMLLSRSMNGAILTYNASDIVPTNIPRVNTHGYIGAPSGKENLLIRACFSNDNIRIVSGSEDAHVTEWDLGSHEIIGKYNGHEGAVVDVDYHPDQKIVVSCSTDSRVIVREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARG3</accession>
    <name>G3ARG3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein BZZ1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_72123</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a role in endocytosis and trafficking to the vacuole. Functions with type I myosins to restore polarity of the actin cytoskeleton after NaCl stress.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the BZZ1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376062.1">
        <property type="nucleotide sequence ID" value="XM_007376000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARG3"/>
    <dbReference type="FunCoup" id="G3ARG3">
        <property type="interactions" value="363"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARG3"/>
    <dbReference type="GeneID" id="18875416"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72123"/>
    <dbReference type="eggNOG" id="KOG3565">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015390_1_0_1"/>
    <dbReference type="InParanoid" id="G3ARG3"/>
    <dbReference type="OMA" id="TPMMEEP"/>
    <dbReference type="OrthoDB" id="8783038at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045010">
        <property type="term" value="P:actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11912">
        <property type="entry name" value="SH3_Bzz1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11778">
        <property type="entry name" value="SH3_Bzz1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000060">
        <property type="entry name" value="Actin polymerization protein Bzz1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035459">
        <property type="entry name" value="Bzz1_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF21">
        <property type="entry name" value="PROTEIN NERVOUS WRECK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="11">
        <location>
            <begin position="4"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="464"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="548"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="527"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="135"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00054085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="607" mass="68101" checksum="D8C1345D6A50355A" modified="2011-11-16" version="1">MAELSIGNELKDAYKPTIKWINNNINFLADVDEFYRERANIEKEYSNKLKELCKRHFDKKSKLSASLSVGDEPAVTPGSLESASLVLWTELLSQTEAIASERSKLGTEFSTMIGSNLMSLKGKCERIVRSTDSINEFLTKEKKGVEDEVTKAKKAYDLLCQATESAREKQERNSGSDKYAKRLEEKEVEMNNGKNEYLIKINTANRLKDKYYYQDIPELLDYLQELNQDRVTLVNKLLKNASIIERNANDKVKEKLHNIDQTVDQNKPHLDVAMFIKHNQLDWKEPADFYFIPSSIWHDDESLIIKEPELTVLKKRLNVASGQYSKYEQSCLDIKQKLEELTADRKKEGDNLTLKFDASLLGSLSVLEKFIKEDTQRVRNEVEIEIIQNFAGDQDLTYIAPIERKKSKFGSLFKKKDKETGGVASDDSSTHSLSTTRTNTTLSHVGTGIFNLRRNKTVTSTNGDSSGNAKALYAYQAAGNDETSITPGEQVTVLEPDEGGWTLIQAAAGKGLVPTSYLEITQSDNVSMSSLQKPKKQGPSVAPRRGAKKVSYVQALYDYSADGDDEITIHAGDKIVVTQEDTDGSGWTEGELNGNKGVFPTSYVKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARI9</accession>
    <name>G3ARI9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15 18">Delta(24(24(1)))-sterol reductase</fullName>
            <ecNumber evidence="15 18">1.3.1.71</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">C-24(28) sterol reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Sterol Delta(24(28))-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">SPAPADRAFT_61883</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ergosterol + NADP(+) = ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16933"/>
            <dbReference type="ChEBI" id="CHEBI:18249"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.71"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:18503"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="17">Steroid metabolism; ergosterol biosynthesis; ergosterol from zymosterol: step 5/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the ERG4/ERG24 family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.71" evidence="15 18"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376088.1">
        <property type="nucleotide sequence ID" value="XM_007376026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARI9"/>
    <dbReference type="FunCoup" id="G3ARI9">
        <property type="interactions" value="115"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARI9"/>
    <dbReference type="GeneID" id="18874093"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61883"/>
    <dbReference type="eggNOG" id="KOG1435">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015631_3_1_1"/>
    <dbReference type="InParanoid" id="G3ARI9"/>
    <dbReference type="OMA" id="QLPYFCN"/>
    <dbReference type="OrthoDB" id="5326588at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000246">
        <property type="term" value="F:Delta24(24-1) sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050614">
        <property type="term" value="F:Delta24-sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000003">
        <property type="entry name" value="C-24(28) sterol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001171">
        <property type="entry name" value="ERG24_DHCR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018083">
        <property type="entry name" value="Sterol_reductase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257:SF31">
        <property type="entry name" value="DELTA(24(24(1)))-STEROL REDUCTASE ERG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01222">
        <property type="entry name" value="ERG4_ERG24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01017">
        <property type="entry name" value="STEROL_REDUCT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01018">
        <property type="entry name" value="STEROL_REDUCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 18" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11 18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="9 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="14 18" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="10 18" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="13 18" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="167"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="231"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="290"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="325"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="422"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU369120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EGW31310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="469" mass="55241" checksum="731CA4A083095FE0" modified="2011-11-16" version="1">MSVESSEDQTTLVESSTPQPPRGYLAKDQIEYEFGGPVGAIGMMVGFPLLMWYMWLSAQFYNGKPAWPSADQSWSDFITVDLWNLFVEHGLPSFKVWLFFTVFIVTQAVFYLTLPGVWTKGQPLTHLNNRQLPYFCNAIWSFYTSIVLSLVLHFTGLWKITFILDNFGAIMTTAIFYGISLSIILYLICLYVTGDYHRMTGNHIYDMFMGAPLNPRIGQYLDLKMFFEVRIPWFILFFTSLALCFKQYETYGYVSPQAVFVLYAHWLYANACAKGEELIVPTWDMAYEKFGFMLLFWNIAGVPFTYCHCTLFLAYHEPKEYQWPVYYNILLFVVLTVAYYFFDTGNRQKNSFRRTMAGNTTLRKTFPYLPYSDLVNPSYIKCENGSVLLTDGWYVYARKMHYTADYIQTLTWALMCGFSSPFPWFFPVFFFIVLVHRAYRDQRKCQKKYGKDWDRYLEKCPYMFIPYVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARN4</accession>
    <name>G3ARN4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH La-type RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_67808</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376565.1">
        <property type="nucleotide sequence ID" value="XM_007376503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARN4"/>
    <dbReference type="STRING" id="619300.G3ARN4"/>
    <dbReference type="GeneID" id="18875185"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67808"/>
    <dbReference type="eggNOG" id="ENOG502T73H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_826382_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARN4"/>
    <dbReference type="OMA" id="YKIATRT"/>
    <dbReference type="OrthoDB" id="340227at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07323">
        <property type="entry name" value="LAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF132">
        <property type="entry name" value="LA-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="4">
        <location>
            <begin position="196"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="27"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="37"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="133"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW31787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="289" mass="32742" checksum="103112E32F3961D5" modified="2011-11-16" version="1">MVNRLLPAPPPTVNAWSLNQLIENNPPVESNDEVVPSLTSPLISPEDSSPFTPHSAPPLTKRKSNKKNSGSVMIRIGKNTTIKKPTKFPQNQGNIKGGTGAYKYKIATRTDNYHYTNPSIVYPMDTSRDESESSPIQEQTSEGQFTSPLQTQLSSPIEYEGPVSPLEGQFPLVYPSPFPIPVFPIYPIYEYYPEVQSPKSSKHDIIKRQLEYYFSTENLCKDLYLRSLFNPIDGSVKLQDLIQFNRLKNLTKNGTDITLLIDAISDIDSLQLLENEPRVRLKNWRDWVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARQ5</accession>
    <name>G3ARQ5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Histone deacetylase</fullName>
            <ecNumber evidence="2 10">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_140568</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the histone deacetylase family. HD Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="2 10"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376586.1">
        <property type="nucleotide sequence ID" value="XM_007376524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARQ5"/>
    <dbReference type="FunCoup" id="G3ARQ5">
        <property type="interactions" value="1064"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARQ5"/>
    <dbReference type="GeneID" id="18870300"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140568"/>
    <dbReference type="eggNOG" id="KOG1342">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007727_7_4_1"/>
    <dbReference type="InParanoid" id="G3ARQ5"/>
    <dbReference type="OMA" id="GKIMEWY"/>
    <dbReference type="OrthoDB" id="1918432at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032221">
        <property type="term" value="C:Rpd3S complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10004">
        <property type="entry name" value="RPD3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000001">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF10">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037913">
        <property type="entry name" value="His_deacetylse_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="15">
        <location>
            <begin position="37"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="405"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW31808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="468" mass="52786" checksum="E39CE9149953AA0F" modified="2011-11-16" version="1">MYVQLPFDELKVDPTQKKRIAYFYDADIGNYAYGAGHPMKPHRIRMAHSLIMNYGLYKKMEIYRAKPATKQEMCQFHTDEYIDFISRVSPDNLELFAKEQIKFNVGDDCPVFDGLFEYCGISGGGSMEGAARLNRGKCDIAINYAGGLHHAKKSEASGFCYLNDIVLGIIELLRYHPRVLYIDIDVHHGDGVEEAFYTTDRVMSCSFHKYGEFFPGTGELRDIGVGKGKYHSVNVPLRDGIDDATYKSIFEPLITKIVEWYQPSAIVLQCGGDSLSGDRLGCFNLSMSGHANCINFVKSFNIPMMVLGGGGYTMRNVARTWAFESGLLNNVVLPSELPYNEYYEYYAPDYKLDVRPSNMYNSNSPEFLDKILTNIISNLENTKHAPSVQMNVVPNDAEELGDVEEDTKEAMDTKGGSELARDAQIQPDNEFYEEDDKDKGEKNIDETTNGTSKLTDEEMNEIEKLNSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARY3</accession>
    <name>G3ARY3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_72544</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376610.1">
        <property type="nucleotide sequence ID" value="XM_007376548.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3ARY3">
        <property type="interactions" value="282"/>
    </dbReference>
    <dbReference type="GeneID" id="18875441"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72544"/>
    <dbReference type="eggNOG" id="ENOG502QSQ9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_252746_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARY3"/>
    <dbReference type="OMA" id="WQSHHKV"/>
    <dbReference type="OrthoDB" id="10001928at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011498">
        <property type="entry name" value="Kelch_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503">
        <property type="entry name" value="MCG48959-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503:SF2">
        <property type="entry name" value="NEGATIVE REGULATOR OF SPORULATION MDS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07646">
        <property type="entry name" value="Kelch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="460"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="610"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="788"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1021"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1170"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1268"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1465"/>
            <end position="1515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="478"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="616"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="646"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="671"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="836"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1049"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1096"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1268"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1286"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1307"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1331"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1360"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1483"/>
            <end position="1501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1515" mass="167709" checksum="59205A5FE82B1EF9" modified="2011-11-16" version="1">MGSLIPTLSSCHPLQLPPAEKDDRLNLNVRTGSASTLYNSFIFTYGGLTIALELSNYTVEEINRTFHNKVNETNLRFKTIDKYLSGELFYMNLIERHWTRVAFDNSLAPSRPSPRLLHQMCALNNCIYLFGGLVLPDFTNGDASFLSATNDLWEFNLEKNKWTLLHDGVDYSRDKAIPAPRYNHKMATISSLSFVNKRDHFGIFICGGRDQRGREIYDNVLFDLVEKRYVGTSPLQLSVTTSNPKKDEAHGLNNFKANEDHLLSLNSTTGMILSFVEEVESEQKIDGKTKTLHTSTTHEESLIVYSATKEDHHNSLVSFKLGKSIKSGRTLPVHRKKIVNNLTTPFNLRYPSGGLFGQNIVVTGFLPGEIDISIFIYNKPTGKWSRLNVVCNHDYGSHRFWGGFVWQSHHKVVLLGNYLTSRTTSSVRYFTIMVTVSLPITNILASSELEGGHFHREDGTKVYRRKSSTTTDNDEFSSDTSSSEGASDGNEEDDLLHTRRHSITTLATDKSPAAISFTEYVHYAAPKVTFTTIRSVFPPAAITLGRNAFERYGDMVSDFELVSSNGDRIPVCLVVLMERWGGYFVQLLSKGYVQAVDKFETQQAMGLYEGQRLRSKTSNSSESPSSSPRPPLKTNTSAVDPHQINFRRSSMTSSHFNTNPLFPSDLQDIPPQLPLPTESLPPVPATPVSFRSSSRKGSQDLGSPRASLLHTLTVLRNIPTSRSPRDSPFASPRASLSGQGGIAPGINHPVNSSAGELFGSPFSNFRSPGRTLSAAATNRRRSIDVLMAGEPAELTKPSDSLDLRTEKASLSTMNSEGTRDSSIAEGYKSSDDDSSRTGSQISSGSISAPTPFTPSDSKEGEIDKQEGDVFENALLNFDNIESNTFRMEPSLIPRKLYFPFPTITVKAFCEYLYTGQIGNKWILAPTVLDNLLIAKQYRVPLLYDLISEVLFGIIGRKEAYVVGEGNKLKRKYFDLMKQLGAPTDPNFSFPLDEYEGFMDTVDDAYLDIGLLKKISRTRKTRAASKSSKKSTSSLFAGMDEESLSKKRSSKELSPKEVVETDQELSPKSRGDENDNTLERNDEDSDGHDDTFEGAPENMENVVEKVTTNVEEEDEVNVSDEREMNSNSEEEQEIELGYLDTYANSGIPIGPRSKSVFDKHSFLFQELKQAEEEIEAEDEDERGRGASTSTKDTDYAERNANYALGELISPDAPIPSDYSIDLIFEAGTIVSDLKLMLRAYNLRAMTRILKRTRADIERAISELEELVESKKHHDDLKLKRATGTESVKYENRKSSGIDPHTVAHETSESPSETSAMIRSESSSFGFHDMVPSSSNNSVNTTNSQRSNGGGSTTGSAHPISRTGSHTSLRGLTSLTPFSKSKERLARNESASDLVSMDKVPMTFDKSIRKKFHTVGTGVLGRVPTTKAEEVITSSASSIKSAATAATSATTSVNKKYGLFHLHRREKTSSGDELSVESNKLERASSQVSLSSGSSKNESTTGTTHKRRGRGLFGLMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS67</accession>
    <name>G3AS67_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62920</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377059.1">
        <property type="nucleotide sequence ID" value="XM_007376997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS67"/>
    <dbReference type="STRING" id="619300.G3AS67"/>
    <dbReference type="GeneID" id="18874526"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62920"/>
    <dbReference type="eggNOG" id="KOG0834">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039185_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS67"/>
    <dbReference type="OMA" id="NTWLFSE"/>
    <dbReference type="OrthoDB" id="25002at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="1">
        <location>
            <begin position="44"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="311" mass="36427" checksum="588DE2156202443A" modified="2011-11-16" version="1">MTEPTNIPTEEDTGPVIPVTKHHDETWIFEEDAFWNHSPSRNQKMTISQELKAKESIHDFVIRLGSKLKLDARTILSATIYLHRFYMRLPITSSKYYVASAGITISCKLNDTYRQPDKIALQACNLKNTSGKPIDEQSDMFWRWRDQLLYREELILKALNFDLNIESPYEVRDRLIEDNFEHQDTVFAKKRVDLLKNSVTLIETLSSLPILICYDINTLYATAIIITFFEFKAKEPDLQLPNRFLTDELEVDLQTCMKCFLFIKKLLKYSQQDQHFVSNKVAARRVLSIPTVEFEQIIKRMEEPRIKLAQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASL7</accession>
    <name>G3ASL7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SMP-LTD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_62567</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376736.1">
        <property type="nucleotide sequence ID" value="XM_007376674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASL7"/>
    <dbReference type="STRING" id="619300.G3ASL7"/>
    <dbReference type="GeneID" id="18874380"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62567"/>
    <dbReference type="eggNOG" id="ENOG502QUUW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1305536_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASL7"/>
    <dbReference type="OMA" id="RTWFIDR"/>
    <dbReference type="OrthoDB" id="5599157at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120013">
        <property type="term" value="F:lipid transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015917">
        <property type="term" value="P:aminophospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070096">
        <property type="term" value="P:mitochondrial outer membrane translocase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd21671">
        <property type="entry name" value="SMP_Mmm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019411">
        <property type="entry name" value="MMM1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466:SF0">
        <property type="entry name" value="SMP-LTD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466">
        <property type="entry name" value="TEX2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10296">
        <property type="entry name" value="MMM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="10">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="211" mass="23314" checksum="5D8C0EECBFD59559" modified="2011-11-16" version="1">MPEFLGKINLTEIDIGDDFPIFSNCRIKHSTGGRLEAKIDVDLSDTLTLGIDTKLLLNHPRALTAVLPVELTVSIVRFSGCLTVSLINTNDPEFVDLASHNGGKGNGSTINGSAQKSNSSKEGAGTALMFSFSPDYRLEFIVKSLIGSRAKLQDVPKISELIESQLRTWFIDRCVEPRFQVVRLPSLWPRSKNTREPVKTKVDDETGRKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASY7</accession>
    <name>G3ASY7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Transcription initiation factor IIA subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_142741</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TFIIA subunit 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376802.1">
        <property type="nucleotide sequence ID" value="XM_007376740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASY7"/>
    <dbReference type="FunCoup" id="G3ASY7">
        <property type="interactions" value="498"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASY7"/>
    <dbReference type="GeneID" id="18870479"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142741"/>
    <dbReference type="eggNOG" id="KOG3463">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112964_3_1_1"/>
    <dbReference type="InParanoid" id="G3ASY7"/>
    <dbReference type="OMA" id="QYYELYR"/>
    <dbReference type="OrthoDB" id="586585at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000979">
        <property type="term" value="F:RNA polymerase II core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10014">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10145">
        <property type="entry name" value="TFIIA_gamma_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.190:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIA subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.190">
        <property type="entry name" value="Transcription factor IIA gamma subunit, alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009083">
        <property type="entry name" value="TFIIA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003194">
        <property type="entry name" value="TFIIA_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015871">
        <property type="entry name" value="TFIIA_gsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015872">
        <property type="entry name" value="TFIIA_gsu_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10966">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02751">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02268">
        <property type="entry name" value="TFIIA_gamma_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009415">
        <property type="entry name" value="Hum_TFIIA_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47396">
        <property type="entry name" value="Transcription factor IIA (TFIIA), alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50784">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor IIA gamma subunit N-terminal" evidence="9">
        <location>
            <begin position="6"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor IIA gamma subunit C-terminal" evidence="10">
        <location>
            <begin position="66"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR009415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="125" mass="14026" checksum="0C32979A695B7E81" modified="2011-11-16" version="1">MSAPAYYELYRRSTIGVTLTDALDTLISDERIPPQLANRILTNFDRIISDLLKHESNIAKSKLTFKGDLDTYRFCDDVWTFIIKNVLIKMSDVTGGGSGNDSVDTEVQVEKFKIVACNSRKAGEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASZ9</accession>
    <name>G3ASZ9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytoplasmic tRNA 2-thiolation protein 1</fullName>
            <ecNumber evidence="6">2.7.7.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Cytoplasmic tRNA adenylyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">NCS6</name>
        <name evidence="6" type="synonym">CTU1</name>
        <name evidence="10" type="ORF">SPAPADRAFT_142345</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="6"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376814.1">
        <property type="nucleotide sequence ID" value="XM_007376752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASZ9"/>
    <dbReference type="FunCoup" id="G3ASZ9">
        <property type="interactions" value="469"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASZ9"/>
    <dbReference type="GeneID" id="18870440"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142345"/>
    <dbReference type="eggNOG" id="KOG2840">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026481_1_3_1"/>
    <dbReference type="InParanoid" id="G3ASZ9"/>
    <dbReference type="OMA" id="MGKCERC"/>
    <dbReference type="OrthoDB" id="198857at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002144">
        <property type="term" value="C:cytosolic tRNA wobble base thiouridylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103016">
        <property type="term" value="F:tRNA-uridine 2-sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="CTU1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000188">
        <property type="entry name" value="Cytoplasmic tRNA 2-thiolation protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03053">
        <property type="entry name" value="CTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056369">
        <property type="entry name" value="CTU1-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032442">
        <property type="entry name" value="CTU1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020554">
        <property type="entry name" value="UPF0021_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807">
        <property type="entry name" value="ATPASES OF THE PP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807:SF12">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16503">
        <property type="entry name" value="zn-ribbon_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01263">
        <property type="entry name" value="UPF0021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal" evidence="8">
        <location>
            <begin position="68"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Cytoplasmic tRNA 2-thiolation protein 1 C-terminal" evidence="9">
        <location>
            <begin position="328"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="71"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="380" mass="42332" checksum="29E6BF126B257B45" modified="2011-11-16" version="1">MATITTTTMEKKPIKVSALCELCHSRKAVMKRPKNLQKLCKECFYHVFETEIHNTITSAKLFSPGDSVAIGASGGKDSTVLASILKTLNERYNYGLNLVLLSIDEGIKGYRDDSLATVKRNQKQYQMPLEIVSYKDLYDWTMDEIVSCAGIRSSCTYCGVLRRQALDRGAAKLGINHVVTGHNADDMAETVLMNILRGDVARLEKSCSIMTESVGSPVKRSKPFKYTYQKEIVLYAHYKKLDYFSTECSYAPEAFRGTARELLKSLEAIRPSCIMDIIYSGEHLSLAPKKKKTTVSYKNKKKLNAETEINSDGSVSLPKKQEFKDGNICEKCGYLSSNKICKACILLAGLEMNRPKVSIDNNTAIDGAAKLSKTLEQLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT00</accession>
    <name>G3AT00_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62642</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376815.1">
        <property type="nucleotide sequence ID" value="XM_007376753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT00"/>
    <dbReference type="GeneID" id="18874416"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62642"/>
    <dbReference type="HOGENOM" id="CLU_2905614_0_0_1"/>
    <dbReference type="InParanoid" id="G3AT00"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="62" mass="7100" checksum="9D6DA52231F04F66" modified="2011-11-16" version="1">MLYEIAIFLIISFVLVYVVPLVISKHDVKDVLDDIQEKKHTKPRSSSIKEFADDVLDLHGMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATE7</accession>
    <name>G3ATE7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_142653</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376943.1">
        <property type="nucleotide sequence ID" value="XM_007376881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATE7"/>
    <dbReference type="GeneID" id="18870466"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142653"/>
    <dbReference type="eggNOG" id="ENOG502RQ79">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122011_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATE7"/>
    <dbReference type="OMA" id="TYHRAAN"/>
    <dbReference type="OrthoDB" id="4088176at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="147" mass="16876" checksum="F6B58DB721EC4712" modified="2011-11-16" version="1">MSEKEQLKQQEKEGSYNRPPMDAPPPYEEMQPQQQPQQQQYQQPYYDNPNQGYNPGYQQPGYNPRYPPANAYTIQPDKVNIAIAPPQHVNPQYPEFLAREQQRIQMGDYPDNYGKHGAPLNKGKTSKNAHKGFPGRSSATYHEAANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATG3</accession>
    <name>G3ATG3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">General negative regulator of transcription subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_72826</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376959.1">
        <property type="nucleotide sequence ID" value="XM_007376897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATG3"/>
    <dbReference type="FunCoup" id="G3ATG3">
        <property type="interactions" value="159"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATG3"/>
    <dbReference type="GeneID" id="18875457"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72826"/>
    <dbReference type="eggNOG" id="KOG2150">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013819_3_0_1"/>
    <dbReference type="InParanoid" id="G3ATG3"/>
    <dbReference type="OMA" id="YKPQTPY"/>
    <dbReference type="OrthoDB" id="293823at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012270">
        <property type="entry name" value="CCR4-NOT_su3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007207">
        <property type="entry name" value="Not_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04065">
        <property type="entry name" value="Not3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005290">
        <property type="entry name" value="NOT_su_3_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0678">Repressor</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-Not complex component Not N-terminal" evidence="13">
        <location>
            <begin position="3"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="14">
        <location>
            <begin position="448"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="243"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="37"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="268"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="288"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="342"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW30926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="592" mass="66649" checksum="A76909A8AFDA32E4" modified="2011-11-16" version="1">MSARKLQQEFDKTNKKIAEGLSSFDDIYTKLTTTEISSQKEKLESDLKKEIKKLQRSREQLKSWLSDSSIKLDKNLLQENRTRIEHAMDQFKDLEKSSKIKQFSNEGLELQSQKTKFSRFGGDGDDSKRVEALNYINDIIEQLNQQNETMEQEIESLGGQSKKGKGGASTSYAVQSSIDDFKYKIERNNTHIEKLENILNNLEEDKLDPAKIDDIKDDLEYYVENNQEEDYVEYDEFYDQLEVDEDEDVVEVQGSLAQMAAETINEEQQQQQQQQQQQPQPQPQSAKPQPGSATPAAAPATVAQGSSPSPSTAGATTATSSGSTPNKRKPSIVPAPAPPPITAYSSAIKAAQQQIQNTPPPSNVAPATASAAPTTASSSASSTPAKMPPPGLNQPKSQSASPLISKPKLAGSDDDSDTDAISRISSIIQSRLNNPLPFTSISNLVESSLLNCPDSFDAEKPRQYIPINIHPSSIDYPQEPMYELNSANYMRKFDNDTLFFCFYYSEANDSFGKWNAAKELSKRGWIFNTELKQWFLKDNKNRSMSVIQKEEEAEHATVDDDEANYKYFDYEKTWLTRRRENYRFSKELRKTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATI3</accession>
    <name>G3ATI3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flo11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_62854</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376979.1">
        <property type="nucleotide sequence ID" value="XM_007376917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATI3"/>
    <dbReference type="GeneID" id="18874494"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62854"/>
    <dbReference type="HOGENOM" id="CLU_816759_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATI3"/>
    <dbReference type="OMA" id="TRVYPIT"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442697" description="Flo11 domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="130"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="340" mass="35832" checksum="734D95CA209122F3" modified="2011-11-16" version="1">MKFSAFLLPTLLVISHAFPVVKKRDEVLTTITNVYTDYTTTTRSQSTFTNTIVHMETVTTTKITGTVTVTLYPGQSIPTQVQKVETVSVSPKAHTKKKGDDKVVVKEVETATVDPPATSAEAVTTKADGPTTSTESTSTDSSDNDKTTTPTTSSTSGTPESTLADPTTSSSDDDTPSTSNDPTTSNDPTTSNDPTTSNDPTTSNDPTTSNDPSTSNDPTIYPTTSSSDNDTTTTSSDPTVDPSTSSSDINTPTADPSTSHPTSTAPSSTKSPSITATPTTHTPTTTPAPSTFPSSWIIDSITTETTDGVCYVDYYYHYDTETDEPPITSTSTIYTTVTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATN5</accession>
    <name>G3ATN5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_52184</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377031.1">
        <property type="nucleotide sequence ID" value="XM_007376969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATN5"/>
    <dbReference type="GeneID" id="18871638"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52184"/>
    <dbReference type="HOGENOM" id="CLU_888939_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATN5"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442701" evidence="2">
        <location>
            <begin position="20"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="313" mass="35848" checksum="5817B78839D6092D" modified="2011-11-16" version="1">MWMLAAFIVFTASVLFGSSQPDSTSIDTDEAKLNQETNNTPVTIPSKESFTSKLYQNPTTEAANDTSKNKHKSIHKHSIRKLKQFFKSRFHKKNKSKKQQVNQPLNNFSGSLVQHKQESCSPSNSLEVFSSNSEKEYNTPDSTLASKSSRTSSKRERLKEWFAKKSEPECYQTEFKVKLKPLNPASKSGPTIHNLSISGNHNKRSADCGKKVTFSSLVQDVKSKLVPLSENIPRVEPVIKSPVKSNYKSILKVKTNPNYDKEYFEDYDFLFRAWCELEDMKASLSPEARQTKRETTTLLRKKQLQRFYKQEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATX9</accession>
    <name>G3ATX9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_157271</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377325.1">
        <property type="nucleotide sequence ID" value="XM_007377263.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATX9"/>
    <dbReference type="FunCoup" id="G3ATX9">
        <property type="interactions" value="603"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATX9"/>
    <dbReference type="GeneID" id="18871071"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157271"/>
    <dbReference type="eggNOG" id="KOG0273">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007609_1_1_1"/>
    <dbReference type="InParanoid" id="G3ATX9"/>
    <dbReference type="OMA" id="KWNKCGN"/>
    <dbReference type="OrthoDB" id="1367865at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034967">
        <property type="term" value="C:Set3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045183">
        <property type="entry name" value="Ebi-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846:SF2">
        <property type="entry name" value="F-BOX-LIKE_WD REPEAT-CONTAINING PROTEIN EBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="203"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="418"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="572" mass="63115" checksum="9A380561BE4C4AB1" modified="2011-11-16" version="1">MSLTSRELNYLVWRYLQESGYDLSAYALDQASLCSQYENSTNHDIITKIPPGCLVNLIQKGILFSIAEEEAKHESETKHEQEEVPNGKLNLLNALVHDHLKAVDPEDKRFMLRSELNGSEDKDNMEGIEKTDTEELSEPIQHAIDFTTKIIQPVIKFSPSLTCDWHPITKVFAYGKNEGSAVINAIKDGQIIESVILNHSNLLEASHNDVNIVSWSPLGNLIVTGGINGELRAWSPDGKLKNIANIVAEDVTSTKARAIIVSLYWSSSGQYLISVDSRNQICLWDGNTLSLIKQITDTTAATAAATAAATTTTTTTPSSYITSCAWLHEDKFALTTCKNNIKIHDIVQNAYGTIEIQPIGVLSGHEHSISLMKFNKNNKLLVSCSDFDYSIKIWRGSKGAGSTGATSAEESLDINIGDSKHNSPIVGLEWLEDDNLLSVSMDGVLNIWNSTTGKSLKHCQLLRKENFKTEQENADLIKDVLIFNVVLSPSKKYLAVGDDYCRITIWDMSLEGPKDLVRCLGVYSPEIAQKEGEQSNGHITENVGICDLKWDNESTYVSGSFNGIESVIVSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATZ9</accession>
    <name>G3ATZ9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_52464</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377346.1">
        <property type="nucleotide sequence ID" value="XM_007377284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATZ9"/>
    <dbReference type="STRING" id="619300.G3ATZ9"/>
    <dbReference type="GeneID" id="18871686"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52464"/>
    <dbReference type="HOGENOM" id="CLU_1367005_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATZ9"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442569" evidence="2">
        <location>
            <begin position="18"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="200" mass="19823" checksum="ECAAEC6BB246B6A9" modified="2011-11-16" version="1">MTIIFPTSMILTAMCWGGRFTSTTALGTASSNTAALGATSSGAASSGTTSFGTASSNTAALGATSSGATSSNTAGLGATLGATSLGSASSGSASSGSTGFDAATACSGTACFGNSVFGCAAFGCAMFVGTMFGAAACFGTTGFGGAMFGSTVLVGTMFVGTMFGAAACFGTTGFWWCYVWQYCACWYYVCWYYVWSYYLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU52</accession>
    <name>G3AU52_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Lysophospholipase</fullName>
            <ecNumber evidence="2 10">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_157441</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the release of fatty acids from lysophospholipids. Phospholipase B may well contribute to pathogenicity by abetting the fungus in damaging and traversing host cell membranes, processes which likely increase the rapidity of disseminated infection.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the lysophospholipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="2 10"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377399.1">
        <property type="nucleotide sequence ID" value="XM_007377337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU52"/>
    <dbReference type="STRING" id="619300.G3AU52"/>
    <dbReference type="GeneID" id="18871079"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157441"/>
    <dbReference type="eggNOG" id="KOG1325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014602_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU52"/>
    <dbReference type="OMA" id="FASCLAC"/>
    <dbReference type="OrthoDB" id="4084751at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000010">
        <property type="entry name" value="Lysophospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF33">
        <property type="entry name" value="LYSOPHOSPHOLIPASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9 10" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6 9 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005131592" description="Lysophospholipase" evidence="10">
        <location>
            <begin position="17"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="PLA2c" evidence="11">
        <location>
            <begin position="29"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW30428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="595" mass="64195" checksum="7B66C397AAFD966E" modified="2011-11-16" version="1">MYLIPQILLLLQVVLATSPTKGYAPGAVNCPGGSLTRAATGINTQEENYITNRNPITKSALGTFLSNAGLTDFNVAAFLGEASPSIGLAFSGGGYRAMLTGAGELAALDSRTGSKDKTLAGILQASSYIAGLSGGSWLVGSVASNNYLSIDQILGNGNLWNINSILDYYGINIISDGLMWTEIAEQVNDKSKAGFETSITDPWGRALSYQLLSNFNDKGDAVLWSDITTNSAFTSFQMPFPILIADGRVAGTSIVSSNSTVFELTPYEVGSWDPTLKSFVQTKYMGTALDGGKPTGKCYNGYDNAGFFMGTSSSLFNEALLSLSSSGLPSFLTKLISSIANSDADVADYKPNPFYKSTNQDTTISKASVLNLVDGGEDGQNIPILPLLNRNVDVIFAHDNSDNINNWPDGSSLINTYERQFTSQGQNIAFPYVPGQSTFRNLNLTSKPVFFGCDAQNLTSLTENIYDVPLVVYLPNRPFTYWSNTSTYKLDYSNAARNAMIQNGYEISTRKNGTLDSEWAACIGCAIIRREQERLGQTQSAQCQQCFKNYCWDGTIYQGPDLGDNYSDNGLTKSATYYNSNNVAGFSDGGTDLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU68</accession>
    <name>G3AU68_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_63280</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetate uptake transporter (AceTr) (TC 2.A.96) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377415.1">
        <property type="nucleotide sequence ID" value="XM_007377353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU68"/>
    <dbReference type="GeneID" id="18874674"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63280"/>
    <dbReference type="eggNOG" id="ENOG502QUJS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051062_1_0_1"/>
    <dbReference type="InParanoid" id="G3AU68"/>
    <dbReference type="OrthoDB" id="3648309at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015123">
        <property type="term" value="F:acetate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051633">
        <property type="entry name" value="AceTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000791">
        <property type="entry name" value="Gpr1/Fun34/SatP-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038013">
        <property type="entry name" value="AceTr_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123:SF1">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01184">
        <property type="entry name" value="Gpr1_Fun34_YaaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="234" mass="25174" checksum="896F3902BF562499" modified="2011-11-16" version="1">MSDTENRSSVEDVKRVSSDSLQNHSSDPHWPIQPAVHQFADAGALGLCGFCMTAFVTGCYFAGTKSIAIPNVLVSLATFYGGTIEVLCGIWELAKGNTYNGTLHTSLGSLWLSLASIYIPSFGIIEAYAEAPEQLTNALGFYFLGWCVFAVVIFLASLKSTYPVIATLVVLLTALLCLSGGFLSGSANALKAGGILLIVTSFFGWYIVFGFIATKQNSYIRTWTLPVHIFGKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUA5</accession>
    <name>G3AUA5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Methionine aminopeptidase 2</fullName>
            <shortName evidence="9">MAP 2</shortName>
            <shortName evidence="9">MetAP 2</shortName>
            <ecNumber evidence="9">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MAP2</name>
        <name evidence="13" type="ORF">SPAPADRAFT_63305</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9 10">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="9">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="9"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377453.1">
        <property type="nucleotide sequence ID" value="XM_007377391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUA5"/>
    <dbReference type="FunCoup" id="G3AUA5">
        <property type="interactions" value="1188"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUA5"/>
    <dbReference type="MEROPS" id="M24.002"/>
    <dbReference type="GeneID" id="18874696"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63305"/>
    <dbReference type="eggNOG" id="KOG2775">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015857_7_1_1"/>
    <dbReference type="InParanoid" id="G3AUA5"/>
    <dbReference type="OMA" id="KGTKCQT"/>
    <dbReference type="OrthoDB" id="7848262at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01088">
        <property type="entry name" value="MetAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03175">
        <property type="entry name" value="MetAP_2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050247">
        <property type="entry name" value="Met_Aminopeptidase_Type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002468">
        <property type="entry name" value="Pept_M24A_MAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018349">
        <property type="entry name" value="Pept_M24A_MAP2_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00501">
        <property type="entry name" value="met_pdase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777:SF2">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01202">
        <property type="entry name" value="MAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9 10" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="12">
        <location>
            <begin position="102"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="22"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="43"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW30482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="415" mass="46488" checksum="9AAB873E272FF869" modified="2011-11-16" version="1">MSAEVEAATANVVEPKVDQLKVEDLSLEDSVTPEPESTDAAPSKKKKPKKKKKKITTIDASYPDHIFPEGEWQEYPLDVNSHRTTSEELRYLDRQSNNKWEDFRKGAEIHRRVRHKAQSSIKPGMSMIEIADLIENSVRAYSGNVHTKEAGIGFPTGLSLNHVAAHYTPNTGDKMVLHKDDIMKVDIGVHVNGHICDSAFTMTFEDTGKYNPLLQAVKEATNTGIKESGIDVRLNDIGAAIQEVMESYEVEENGKFYPVKCIRNLNGHNIGDYVIHSGKTVPIVPNGDMTKMEEGETFAIETFGSTGNGYVITEGECSHYAYMPESDSIRPPSERAKELLDNIKANFGTLPWCRRYLERAGEDKYLFALNQLVRAGIVEDYAPLVDKKGSYTAQFEHTILLHPHKKEVVTRGDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUE5</accession>
    <name>G3AUE5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hsp90 chaperone protein kinase-targeting subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_63357</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC37 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377492.1">
        <property type="nucleotide sequence ID" value="XM_007377430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUE5"/>
    <dbReference type="FunCoup" id="G3AUE5">
        <property type="interactions" value="461"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUE5"/>
    <dbReference type="GeneID" id="18874715"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63357"/>
    <dbReference type="eggNOG" id="KOG2260">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033261_1_0_1"/>
    <dbReference type="InParanoid" id="G3AUE5"/>
    <dbReference type="OMA" id="NYSKWDQ"/>
    <dbReference type="OrthoDB" id="440202at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071852">
        <property type="term" value="P:fungal-type cell wall organization or biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038066">
        <property type="term" value="P:p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030474">
        <property type="term" value="P:spindle pole body duplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.610">
        <property type="entry name" value="Cdc37, Hsp90 binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004918">
        <property type="entry name" value="Cdc37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013873">
        <property type="entry name" value="Cdc37_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013874">
        <property type="entry name" value="Cdc37_Hsp90-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038189">
        <property type="entry name" value="Cdc37_Hsp90-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013855">
        <property type="entry name" value="Cdc37_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12800">
        <property type="entry name" value="CDC37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12800:SF4">
        <property type="entry name" value="HSP90 CO-CHAPERONE CDC37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08564">
        <property type="entry name" value="CDC37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08565">
        <property type="entry name" value="CDC37_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03234">
        <property type="entry name" value="CDC37_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01069">
        <property type="entry name" value="CDC37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01070">
        <property type="entry name" value="CDC37_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01071">
        <property type="entry name" value="CDC37_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101391">
        <property type="entry name" value="Hsp90 co-chaperone CDC37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cdc37 N-terminal" evidence="9">
        <location>
            <begin position="2"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc37 Hsp90 binding" evidence="8">
        <location>
            <begin position="204"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc37 C-terminal" evidence="7">
        <location>
            <begin position="432"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="208"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="215"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="257"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="522" mass="58713" checksum="9053AC636D7F3A9B" modified="2011-11-16" version="1">MPIDYSKWDKIELSDDSDIEVHPNVDKQSFIRWKQRDIHEKRAQRNIEIKSILLQLTMYRALNLRVDYLLESLSESQLLDQKLVLQKLDGQFDKNEKFDYEKLKQEKGDELRKGLRDLTFDQSEIENTPCYNEMIDDLFIQIKDDHADAKSDGAKLKGYLQEHRAKIDDVLSKQTIKLDDLLYQKSMLISSDDIHTGFDRSFLNKNDQDEDAEEVPAPAVAPKQTETVTTTETINASSATPSAAAAASSSTPAAASATPAAASATPAAPAAAPADDETDLHLEKATAEFAKIPPKDLTKIADFLIKHTHIISEQQKDALIMTAFDQQLANDSAGALQTIHQSLLLQYISQLAGPHHNRDQAIRAVKLFISKVADEGSPAHTGFMEDVNNTFKHIQTRCEIIKQEQQELNPDEPEGEELIQLRALDEGTELQVNIPAENTPQYEIFKTKLSPEFQAAIMTQSLDEVNKEFAKLKVDEAEKVLEVFNECGVIGITGYLEDEQEFEELKKEYKEENAEPVEDVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUI2</accession>
    <name>G3AUI2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_63368</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377509.1">
        <property type="nucleotide sequence ID" value="XM_007377447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUI2"/>
    <dbReference type="GeneID" id="18874720"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63368"/>
    <dbReference type="eggNOG" id="ENOG502SWKX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175850_0_1_1"/>
    <dbReference type="InParanoid" id="G3AUI2"/>
    <dbReference type="OMA" id="WCTCISE"/>
    <dbReference type="OrthoDB" id="88410at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34724">
        <property type="entry name" value="OS12G0596101 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34724:SF2">
        <property type="entry name" value="OS12G0596101 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="59" mass="6142" checksum="420611423B67E026" modified="2011-11-16" version="1">MCKPAECGVCKGKTWWGCGQHIPAAMSAAPKDQWCTCESASGAELNGYPPKQGDGKAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUN6</accession>
    <name>G3AUN6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex component SEC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_73278</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the SEC5 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377563.1">
        <property type="nucleotide sequence ID" value="XM_007377501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUN6"/>
    <dbReference type="FunCoup" id="G3AUN6">
        <property type="interactions" value="320"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUN6"/>
    <dbReference type="GeneID" id="18875475"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_73278"/>
    <dbReference type="eggNOG" id="KOG2347">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_321631_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUN6"/>
    <dbReference type="OMA" id="DCSQFYD"/>
    <dbReference type="OrthoDB" id="26242at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029175">
        <property type="entry name" value="EXOC2/Sec5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039481">
        <property type="entry name" value="EXOC2/Sec5_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15469">
        <property type="entry name" value="Sec5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC2/Sec5 N-terminal" evidence="6">
        <location>
            <begin position="57"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU365069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF15469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="901" mass="104636" checksum="602ED89413E06945" modified="2011-11-16" version="1">MNIRPTEEEIKRYYGVNTLHLTNKDQLNYNPLADLDPDEVTSTWSEQSTTSRSFDLQDPLKGPNSNIVSELLDKHIISSKNDPLVYNYLISSQTFNSQKYLTTIHKDTPIEELTHALALLETDIHSHTSELKRAIDDNFLKVINSKKSIDDVLVEFKQQKSKAQADKESSKVFNPTKQRLDVQQDLCSELDSAINNMNTTTSLMIRPISELKAKEVKLMKMIEIVKENSFFFDLPSVLIEALSLNDNEKFLTNYNKFLVEKNKFYTNLQKEREDKLNQAKTSEAVKEIDADISLKHSTVSKVFSPIDSITLQYRQKLIKELLSLDTDIVSRTNTDERNKIISIIDNLMKLNELDTSASHPIADFISNQLNMINKDFEYHINKFDNKFLLHQEKLLDYISGLKQDRQIGSHINYIAEKYNHIIPNEEIFDSSDSLDYSLINETWLVLLNFMEYLDTTLINTLQKILNNYLHYNTIDSDGKIRDAYVEVINQVVLLLVTLFDSVKEETTEANTAIELSPRVYKQFVPYYANSLSTVYYLTKANLKVNRLLTKFGEFIGKIGNASKYPDTNKVIKSLKTSSTNINQKIIEAICSVWINDVTQFYDIEDWKCEKGEQSTTTNLVNIIQYYQTYMVSQISNLIFAKEKSEFQIVSVYPGKRILVSIEIQFMRSLTILVDSIMKKYHIERTISTVDTFKILTMNNLDKLSREIYPKLLRRFDELFDKDLSKQNLKLFVDIDKANLTIIEDILNNEKLYISSRVNLFFKSSKQAKKLKVDGFIYEILIHFVKLINKLKPLTVVEIFIDIINELQLNLLKNILDNIRNFEVSSLGLVNLKLDVNFVLQVFEKSKLLRFNESTYKLVQILLNTIEEKYQEGHALYQYSKQEFDQVLKQNLQDSSNQFGCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUV4</accession>
    <name>G3AUV4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_144040</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377811.1">
        <property type="nucleotide sequence ID" value="XM_007377749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUV4"/>
    <dbReference type="FunCoup" id="G3AUV4">
        <property type="interactions" value="702"/>
    </dbReference>
    <dbReference type="GeneID" id="18870592"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144040"/>
    <dbReference type="eggNOG" id="KOG1443">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022332_1_1_1"/>
    <dbReference type="InParanoid" id="G3AUV4"/>
    <dbReference type="OMA" id="WLMKSFP"/>
    <dbReference type="OrthoDB" id="18894at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015786">
        <property type="term" value="P:UDP-glucose transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
            <begin position="29"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="383" mass="42683" checksum="56548042C8A89380" modified="2011-11-16" version="1">MSKSSHSRKNSDPEPLHASTAGHNGKAALFYIAGWYTFSLSINIYNKWMFGPGLGFRFPLFITSFHQLCLAVLSTLTLYFVPEMRPRIGANHALPQHSHVDDATIRKASFYQSVHIDFRVYVRQMVPCALTSAGDIGLSNVAVSLLSLSLYTILKSSSLMFVLLFGLLFRLEKFNWRLIVIVLVMTVSVTLMTAKPDNIDTSTKGGVYSTLGITLAISAAMLSGLRWSFTQILLKKNPYTPNSIATIFYVSPCMFLALFLLGCYVEGWGNFTSAPIWEIKGVFTTIGLLIIPGVFAFLMMSCEFMLLKVAHLITLSVAGSFKELLTIAVSAAVFGDRLSSLNCVGLVLTFCDVMWYNYYRYIAKTKPQGYTALKEERVEMQKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUW0</accession>
    <name>G3AUW0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_63667</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. In the complex, it is required to stabilize and induce conformational changes of the catalytic subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat TRM82 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377817.1">
        <property type="nucleotide sequence ID" value="XM_007377755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUW0"/>
    <dbReference type="FunCoup" id="G3AUW0">
        <property type="interactions" value="286"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUW0"/>
    <dbReference type="GeneID" id="18874858"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63667"/>
    <dbReference type="eggNOG" id="KOG3914">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022082_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUW0"/>
    <dbReference type="OMA" id="VKHWLFG"/>
    <dbReference type="OrthoDB" id="339900at2759"/>
    <dbReference type="UniPathway" id="UPA00989"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106143">
        <property type="term" value="C:tRNA (m7G46) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106004">
        <property type="term" value="P:tRNA (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03056">
        <property type="entry name" value="TRM82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028884">
        <property type="entry name" value="Trm82"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16288:SF0">
        <property type="entry name" value="TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT WDR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16288">
        <property type="entry name" value="WD40 REPEAT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 6 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="204"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="401" mass="45598" checksum="EC90330BD7F117E1" modified="2011-11-16" version="1">MKHPYQIVIVNKAGTHLFTSVKNTVQVFELASGKKVGEWIDDISPDQVTHQNTQISKKKGEPIMSQPIYNHIRSLLLTGNEEYLVGTTDSDKSTIIFHVDFTGENCLTLSKRQVLAKRPCSISFDAGNENVVVADKFGDVYSIPIDTNEPVPEKELAPILGHVSMLSDVLVTSYKGKQFIITGDRDEHIRISNYPKSYVIKNFLFGHREFVSDLYVSEHDPSVLISGGGDDFICLWHWFDNELLTKVELRDLVEEYLHDGHLPPARFLTEDSKKEISIAKISTLKTDDSYFLIVLVENTRCLLVFEINDDFTVTHNQTITIDNSIVDFSIDESNKRIIASVDTESSQLFEAFKYEQGKFSIDSSLEQSFKSVTDANPFDVESKSDFQQLYYINSMRKRSEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUX7</accession>
    <name>G3AUX7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">XPA C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_144035</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377834.1">
        <property type="nucleotide sequence ID" value="XM_007377772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUX7"/>
    <dbReference type="FunCoup" id="G3AUX7">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUX7"/>
    <dbReference type="GeneID" id="18870591"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144035"/>
    <dbReference type="eggNOG" id="KOG4017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053731_0_1_1"/>
    <dbReference type="InParanoid" id="G3AUX7"/>
    <dbReference type="OMA" id="APKCYEC"/>
    <dbReference type="OrthoDB" id="5368863at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901255">
        <property type="term" value="P:nucleotide-excision repair involved in interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000715">
        <property type="term" value="P:nucleotide-excision repair, DNA damage recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21077">
        <property type="entry name" value="DBD_Rad14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.530.10">
        <property type="entry name" value="XPA C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000465">
        <property type="entry name" value="XPA/RAD14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022656">
        <property type="entry name" value="XPA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037129">
        <property type="entry name" value="XPA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00598">
        <property type="entry name" value="rad14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10142">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-A CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10142:SF0">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-A CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05181">
        <property type="entry name" value="XPA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="XPA C-terminal" evidence="5">
        <location>
            <begin position="260"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="17"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="113"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="402" mass="46842" checksum="E5D507A0E55B2F96" modified="2011-11-16" version="1">MAFTRGTLTEKQRKLIEANRQKAKERLQRKRGEVVTATAKPVQSEQPPQPPQPPPSTRAEPTKTLNQIVNSPVKISSSFVNVSEDGSLKRVRVELTDEQRNRIEQNRLRAIEIRQRKEREKQQSSDASAPPKEGSSSDAQPQDSIRLNKNSQEPTAFKRKFEPPPIRKQDYIEFDFATMKDSRGGFISDDPKNPAPDQQSLDEWKEKQKELQQIRELPPPIDLLNAPKCYECGSIDIDANLMTNFQVRACRKCIKAHPEKYSLLTKTECREDYLLTEPELKDVSLLPRIEKPNPHGYSRMQLFVRFQVEEFAWKKWGGPDKLDEEWERREQQRIKRRDKKYQDSLREMRKRTRAEEYTRKLRNGQGLGERHVHDWSAPVSIGDNTVKRRCVDCGIETEEVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUY9</accession>
    <name>G3AUY9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_68713</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377612.1">
        <property type="nucleotide sequence ID" value="XM_007377550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUY9"/>
    <dbReference type="FunCoup" id="G3AUY9">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUY9"/>
    <dbReference type="GeneID" id="18875237"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68713"/>
    <dbReference type="eggNOG" id="ENOG502R0G6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_480671_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUY9"/>
    <dbReference type="OrthoDB" id="28413at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16457">
        <property type="entry name" value="PH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="1">
        <location>
            <begin position="450"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW29846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="673" mass="77414" checksum="822DB2C5B58952C5" modified="2011-11-16" version="1">MMTLPPATTSSRTINSKLNEILNKATRSPTGSTSDVNKLDQNTVVQYSNLLRANILGKDKQEKVNSIVVLAKLLGCIDVKSVQDLSQLTSILNHDLFHVLFSIITPNMSLETFKAILKICNAMSSIFTTKESYQIVIDTVVKHLDIINIMVDKLHHDFKIMVNSLRFFNDLLSKSLQYHYDSILVIFERLRSQSFFGSIENLNMNDKSLTFLIEQLKSTYIRTLQWLNTMQYDLTSVHHNTILQETLKQLTGSLNEFGNDATPEEFKIAGFGDNPKQFIIENCSILLSIDIIVFLKDSNVTFKKRFHEHLMMYQHKLTFPIYQFIKLSTNLWIDIYDQDEYPNIVSSCLNYDEFIYYTMLNCLRLWQQTKADSEHVDKIFLLITSSIYTIEDDLGSGAKSIEEILDWYMSQDVRLKQVEQIINTHEIAWNPELQEFDKILAQQSLDFIIDQRVVQLLKGSWVYTESYGESLLKSKPSSSNGGIGIGNNSHAAIANKDSARYYFITLSPNRQVIYFKPYLNKPPVNPTIAEMEQNNQYIKLTEIISLKSQKLGEFIGEEDKLKSSRLISIRGTISYEQITLMGNTKPLISFYTDSNVNMYVWLDGIKMLKGITNPDQFSDETAKQLENLIEVRRTIQLLELNNIEPGVIDEDEDVYNLNELDNVTTGFHFMNIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUZ3</accession>
    <name>G3AUZ3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_52711</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative mitochondrial redox protein which could be involved in the reduction of small toxic molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FMP46 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377616.1">
        <property type="nucleotide sequence ID" value="XM_007377554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUZ3"/>
    <dbReference type="GeneID" id="18871712"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52711"/>
    <dbReference type="eggNOG" id="ENOG502SVID">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108996_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUZ3"/>
    <dbReference type="OMA" id="TITIFHN"/>
    <dbReference type="OrthoDB" id="4044803at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012882">
        <property type="entry name" value="Fmp46"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28071">
        <property type="entry name" value="REDOX PROTEIN FMP46, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28071:SF1">
        <property type="entry name" value="REDOX PROTEIN FMP46, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07955">
        <property type="entry name" value="DUF1687"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW29850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="203" mass="23123" checksum="A2D690D55365FD1F" modified="2011-11-16" version="1">MPIMSLLKTLQSTPATITIFHNSKIPLSNKLFRILQSAAHRQSHTPNTPFAAVPVPKFEINVMKDQMPTYDQYLHFLNQCNHDKASLHDSYPFLKGRKKHYYETSHSGHSVTIRGVDMDRIFTEEEYEMIYDAFNKIQELGDNIRDVTPADVFKAPLVVDWDQNKLAHDEATLKTMLAKYVPESEHYSGSGTGIAMDAQPVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV68</accession>
    <name>G3AV68_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63491</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377637.1">
        <property type="nucleotide sequence ID" value="XM_007377575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV68"/>
    <dbReference type="STRING" id="619300.G3AV68"/>
    <dbReference type="GeneID" id="18874778"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63491"/>
    <dbReference type="eggNOG" id="KOG2590">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_869241_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV68"/>
    <dbReference type="OrthoDB" id="4026735at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW29871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="320" mass="35417" checksum="C8CF07AFFFDD5E20" modified="2011-11-16" version="1">MTSPVSYANAAANGAISTVDIDTKSKQEVEAVTESLANTSVEDSSATTEESTEVKEETDDTTKESDSVSSSPSKEKAPKKNLLPAPVPAKSAWGQQKFIKPITNRWVPINAKVILPNTRGKSQQQPRRKKSANASQKTNAKKQGKDGKKKLHLSHQVIPMLVLKNQLVSRPRKNLKTNKLNRLNHNNKARPTVRHLTKTTKSSTNKSLIDVTTTNNNNNNNQMEVTTTDNIKRDTVTQINNNNNNNNNKLHNNRNNQYSQTHNLLNKFQVSSTLNHLFLNLHSKTLTTANSGRNNTKMANTVGTEITVATTIVTVTNNHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVI4</accession>
    <name>G3AVI4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">V-type proton ATPase proteolipid subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_63556</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Proton-conducting pore forming of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Proton-conducting pore forming subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11 12">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1). The decameric c-ring forms the proton-conducting pore, and is composed of eight proteolipid subunits c, one subunit c' and one subunit c''.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Vacuole membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377699.1">
        <property type="nucleotide sequence ID" value="XM_007377637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVI4"/>
    <dbReference type="FunCoup" id="G3AVI4">
        <property type="interactions" value="154"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVI4"/>
    <dbReference type="GeneID" id="18874803"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63556"/>
    <dbReference type="eggNOG" id="KOG0232">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085752_1_1_1"/>
    <dbReference type="InParanoid" id="G3AVI4"/>
    <dbReference type="OMA" id="MSVCPPY"/>
    <dbReference type="OrthoDB" id="1744869at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18175">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18176">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.610:FF:000003">
        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011555">
        <property type="entry name" value="ATPase_proteolipid_su_C_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01100">
        <property type="entry name" value="V_ATP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <keyword evidence="4 12" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="95"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="13">
        <location>
            <begin position="21"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="13">
        <location>
            <begin position="98"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW29933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="163" mass="16734" checksum="36EEEF4093CFE0B6" modified="2011-11-16" version="1">MSEAFGDEYYPAFAPFLGFGGCAAAMILSCAGAAIGTAKSGIGIAGIGTFKPELIMKSLIPVVMSGILSVYGLVVAVLIAGGLSPTERYSLFNGFMHLACGLAVGFSCLASGYAIGIVGDEGVRQFMHQPRLFVGIVLILIFAEVLGLYGMIIALILNTKGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVQ8</accession>
    <name>G3AVQ8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_63842</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377989.1">
        <property type="nucleotide sequence ID" value="XM_007377927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVQ8"/>
    <dbReference type="FunCoup" id="G3AVQ8">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVQ8"/>
    <dbReference type="GeneID" id="18874939"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63842"/>
    <dbReference type="eggNOG" id="ENOG502S5C8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065853_0_1_1"/>
    <dbReference type="InParanoid" id="G3AVQ8"/>
    <dbReference type="OMA" id="AKANPQW"/>
    <dbReference type="OrthoDB" id="2101473at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007396">
        <property type="entry name" value="TR_PAI2-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35802">
        <property type="entry name" value="PROTEASE SYNTHASE AND SPORULATION PROTEIN PAI 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35802:SF1">
        <property type="entry name" value="PROTEASE SYNTHASE AND SPORULATION PROTEIN PAI 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04299">
        <property type="entry name" value="FMN_bind_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010372">
        <property type="entry name" value="PaiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="25052" checksum="C44B82DF05E19C2B" modified="2011-11-16" version="1">MYIPKNYLEENWEQVEYLVKKYPLGTVITTAEDGTIIANHIPFFLRQDEETGKKYLIAHMAKANHQLPTLRAYENVLVVFQSQDTYITPNYYPGKQETHKYVPTWDFACAHIYGNSRIVDDADFVRDQITRLTNQQEAGKPDAWKVSDAPEGFLRAMQKAITGLEIEITRTECKYKMEQKMKKPDIEGVIEGLGNDGQEAIKNLAIDANKRYDAKKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVS1</accession>
    <name>G3AVS1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63853</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007378002.1">
        <property type="nucleotide sequence ID" value="XM_007377940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVS1"/>
    <dbReference type="GeneID" id="18874944"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63853"/>
    <dbReference type="eggNOG" id="ENOG502RQDQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1383992_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVS1"/>
    <dbReference type="OMA" id="PFARIHT"/>
    <dbReference type="OrthoDB" id="4013762at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="231" mass="26591" checksum="9DA10E9BADCADA46" modified="2011-11-16" version="1">MSAVNENENENSQLDGSISPVRDITGNFHSKSKTSIPFARIHTNVLENSPVVIQKADTVKRFPSTTFETRNTDSFSSTDDGDIFYEASPELIDVATLEPEIRYYPTLLEETNYPTINVSLEPHMVKPLAETCELNLESVVNPIDTPPDMFFPSHDNLDADSLVLLNIMKHELKEEEDIKHRVGWFKNWFKFDSQSCWSQFIARPEFIGQTCEDDKSDVYLDKVFEYLYDIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE56</accession>
    <name>G3AE56_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CTLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58809</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373002.1">
        <property type="nucleotide sequence ID" value="XM_007372940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE56"/>
    <dbReference type="FunCoup" id="G3AE56">
        <property type="interactions" value="335"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AE56"/>
    <dbReference type="GeneID" id="18872619"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58809"/>
    <dbReference type="eggNOG" id="KOG2659">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_961591_0_0_1"/>
    <dbReference type="InParanoid" id="G3AE56"/>
    <dbReference type="OMA" id="SLRNQCY"/>
    <dbReference type="OrthoDB" id="2415936at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTLH" evidence="1">
        <location>
            <begin position="112"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="290" mass="33811" checksum="9F4EB174BFD9E426" modified="2011-11-16" version="1" fragment="single">MTNISKDKIDQLILNYFIQEGYQEAAISFSKELNIDITKNAPPVHNTARSTVKENSFINQLGRINKLDENEFSDVAIDYFNREESTPMVKTASLGTKLDHMGGSKLIAGYSTIIPRQEIKLLILKGSITEAIKKISEHFPTILDLNNLLHFKLLRLNLVEMIRNHKLTKNMNQDERQFLDEILTFVRENLINKVSNSFKLLKELEITMSLLCFNFDPNVPNIEDQKDLPQELRNLFNLSLRNQCYRLVNKAILNLYDFDDEENKFETIANNNEDQWKKDNMQIYKGPKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEA1</accession>
    <name>G3AEA1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_147093</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373047.1">
        <property type="nucleotide sequence ID" value="XM_007372985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEA1"/>
    <dbReference type="GeneID" id="18870741"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_147093"/>
    <dbReference type="eggNOG" id="ENOG502RPN3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030508_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEA1"/>
    <dbReference type="OMA" id="PFNRYGH"/>
    <dbReference type="OrthoDB" id="276515at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF36">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="241" mass="27724" checksum="CCCADC59740D74E0" modified="2011-11-16" version="1">MLDIIKELNRYSDDWDYYGAGRIDGEEIGEFVAIIYRKSEWDLVYSDTMWLNHKNPRMSIEGWDATYLRIVSYVTLKHKATNNYINVFNTHFDHIGINSQLGSANLILERIAQINQWPSFIVGDLNSEPKHDAYQLLRQHMADSARLTTPFNRYGHLRSTVTGFEGEVLVEGGQNIDYIFAPKYTSKIDMDPKCSTLNPNSPENKIDLRLTGYGMLHSKFDGIYVSDHRPIVADFSMKGKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEA2</accession>
    <name>G3AEA2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_64731</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373048.1">
        <property type="nucleotide sequence ID" value="XM_007372986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEA2"/>
    <dbReference type="GeneID" id="18874996"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64731"/>
    <dbReference type="HOGENOM" id="CLU_429040_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEA2"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="638" mass="70087" checksum="E24D89FE521B7B53" modified="2011-11-16" version="1">MKFHHFEPYGQPIGFHRPKFFLNKDLKSQTLEEIISKLDSSTYANPPLNKTTTSQSLTADNTSTVETSSSETAADVGVDDIVGAVKAAACESQFTIKQSKRARFKKWMENAKSKGFIVDVSKDAAVDSGFNSNLVDCVCPSKDTNTIDVAPAKTPNTNTSATTSINTAATISTNTAVTTSTKTAATTSTNTTASILSPINTDTTSNINTDAPPSTSVSPPHSVAGSKRAKVKGWFTKIMYGDSQTNIYDVGSNESETDDTDVNDKVIFIDYFTSTSTDSQSIHSKTNTAPSTRISKRAQVKQWFKGFIYPQTDDSDNEQSNIDSALADTDEKVDSNCLGAVGCYLIEPSCANNDVNSFTSVRCLVSEPSYADNSNSIECSSPVYSNIVTDYSTSFFQQLKHWFVRLVFGREIFRDTNSMGVPIDRDYPDNNSDYFDYNSSQPKNSLNVNNELTSNYNGTYDNVCDLAIGGEDSEYPDNNPVTFDNGSSGLEVDFIQRKLHVVEELDESEGSKTFESSNLNPNVIPTYSPSISFTKWNSLSKSNPVTAETFISVDSIQTPNLARSKPFYFVSMDSSNNYKANQEKSCSFMTKYKSNQELLFGVRLCSFMAKNKPNQGLLFGIKPCSLMTKMIYKGNNKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEC8</accession>
    <name>G3AEC8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_64803</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373128.1">
        <property type="nucleotide sequence ID" value="XM_007373066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEC8"/>
    <dbReference type="GeneID" id="18875001"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64803"/>
    <dbReference type="HOGENOM" id="CLU_604332_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEC8"/>
    <dbReference type="OrthoDB" id="4027504at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="242"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="400" mass="47197" checksum="232724610894F052" modified="2011-11-16" version="1">MNSNCWILSFPDEILNIIFYHLNQATTMQAMLVNKKFYQIGKVKLYRCIYVLGSQWPPLAIDAKADTSFHREFTIINTWEQLEVLYLSSRMNLVRYILCRGEQLEFKQAITENWPWIRVELEQCFDGEEESIDALKTDFTKEVIMSNYLPLEQDNYTAKSMIIENEWSVSTNILDIVPHMKGLERIEIVGRSENSLKNLKLTSKTPLHIKWLCVSLLEVDYIDYSELQRLFDLSEITSFEIHLAYLDEASEHENELQRLMKKCTNLKNLSFETTAMLFFPNIMSTLQKHSLEKLYIACASHPPLRSYYLDLYMLNQTNSLTELLVSSTTEGRWSFQEFDRVYKIDREQEEDNTQPEELKLLRDFMRESDTPHPKLRHIVLDRNHYAITNTEHGVQVDPMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEP9</accession>
    <name>G3AEP9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_48644</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373088.1">
        <property type="nucleotide sequence ID" value="XM_007373026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEP9"/>
    <dbReference type="GeneID" id="18871249"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48644"/>
    <dbReference type="eggNOG" id="ENOG502RQD3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_731906_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEP9"/>
    <dbReference type="OrthoDB" id="74835at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="344"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="378" mass="42591" checksum="E6C2F1622947FE29" modified="2011-11-16" version="1">MPNCILTLGKLFKRREKTEKIRKQNVQKGSNSIHTHVQIVQNTPGPSQVRKSSSCRDFFRKLSTRSSRASNSDSPRAPPVIAFEDRILHGGSDIFHTPPSEINNHHNFLADAFLTEDEYGVSLATIPLSPYAISFDDNVLSTSTSKEEDTHPFTTTSSSASVTEANVQIYQPAHVKIVEMPEYEEHHSDNDSLFIEEELNEEDPISQTIISKSGTLEETTTNDRSSIYRLEMVNLVAKHQSIMAKQEREIAHLKKLLLEQRHVNNLLSMATHKGHTSQAAKDPSEFKVRSSFIPIQIISEQNAEEEQPKDLLPPFIAKRQDQVSVRTEMYRKSSSESSWARHGSITSDVSSIMSSSHRHQAQRKLSEDSYKPSPCVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEU1</accession>
    <name>G3AEU1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">UDP-galactose transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_58976</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373183.1">
        <property type="nucleotide sequence ID" value="XM_007373121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEU1"/>
    <dbReference type="STRING" id="619300.G3AEU1"/>
    <dbReference type="GeneID" id="18872703"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58976"/>
    <dbReference type="eggNOG" id="KOG3912">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025028_3_1_1"/>
    <dbReference type="InParanoid" id="G3AEU1"/>
    <dbReference type="OMA" id="FIYKHNV"/>
    <dbReference type="OrthoDB" id="408493at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015165">
        <property type="term" value="F:pyrimidine nucleotide-sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007271">
        <property type="entry name" value="Nuc_sug_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012404">
        <property type="entry name" value="UCP036436"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13146">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13146:SF0">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04142">
        <property type="entry name" value="Nuc_sug_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036436">
        <property type="entry name" value="UCP036436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="272"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="388" mass="43626" checksum="1976E3B09029EB3F" modified="2011-11-16" version="1">MGIGLYLIVAGTLITGAANSLFTKYQDNQCVRNCDNPDFSTHKNFEQPAIQTLQMFVGELSMFLVYYLVYKLGQNRKRQDYVRLNDEDEEEEVKEISIFSNLKLAIPAICDLCGTTLLNIGLVYTPVSIYQMTRGSIVLFVAILSVVFLKRRISKLEWISLLFVTLGVGLVGLSGSRTSPSTDTATSSLIVFGIFLIVLAEMLQAFQFVAEEHILEKHKIIPLQLVYIEGFYGFSILLAVMVVLNFIIGAIQPKQQFESSPFNMVEAFSQVFFNRAVLLSSICIMISIAAFNYCGLSITHRISATARSTVDTCRTLLVWIVAISMGWESFQWLQFTGFAVLVFGTLCFNGVLKPEEWSFVPQVLKSDFNPNERLINVVDEIDEPIERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFR5</accession>
    <name>G3AFR5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Urea amidolyase</fullName>
            <ecNumber evidence="11">3.5.1.54</ecNumber>
            <ecNumber evidence="12">6.3.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_48105</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>urea + hydrogencarbonate + ATP = urea-1-carboxylate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20896"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15832"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>urea-1-carboxylate + H2O + 3 H(+) = 2 NH4(+) + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:19029"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15832"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.1.54"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (allophanate route): step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (allophanate route): step 2/2.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.54" evidence="11"/>
    <dbReference type="EC" id="6.3.4.6" evidence="12"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372466.1">
        <property type="nucleotide sequence ID" value="XM_007372404.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AFR5">
        <property type="interactions" value="179"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFR5"/>
    <dbReference type="GeneID" id="18871179"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48105"/>
    <dbReference type="eggNOG" id="KOG0238">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1211">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002162_4_1_1"/>
    <dbReference type="InParanoid" id="G3AFR5"/>
    <dbReference type="OMA" id="GGMYMCI"/>
    <dbReference type="OrthoDB" id="196847at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004039">
        <property type="term" value="F:allophanate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004847">
        <property type="term" value="F:urea carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043419">
        <property type="term" value="P:urea catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000010">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.10:FF:000010">
        <property type="entry name" value="Urea amidolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053844">
        <property type="entry name" value="AH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014085">
        <property type="entry name" value="Allophanate_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050856">
        <property type="entry name" value="Biotin_carboxylase_complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003778">
        <property type="entry name" value="CT_A_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003833">
        <property type="entry name" value="CT_C_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014084">
        <property type="entry name" value="Urea_COase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02713">
        <property type="entry name" value="allophanate_hyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006043">
        <property type="entry name" value="PRK08186.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00724">
        <property type="entry name" value="urea_amlyse_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02712">
        <property type="entry name" value="urea_carbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866">
        <property type="entry name" value="CARBOXYLASE:PYRUVATE/ACETYL-COA/PROPIONYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866:SF128">
        <property type="entry name" value="UREA AMIDOLYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21986">
        <property type="entry name" value="AH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02626">
        <property type="entry name" value="CT_A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02682">
        <property type="entry name" value="CT_C_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00796">
        <property type="entry name" value="AHS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00797">
        <property type="entry name" value="AHS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160467">
        <property type="entry name" value="PH0987 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0092">Biotin</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="18" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="17">
        <location>
            <begin position="622"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="16">
        <location>
            <begin position="741"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="15">
        <location>
            <begin position="1746"/>
            <end position="1822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00066801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW35054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1822" mass="200645" checksum="F2505C46D5A4AE94" modified="2011-11-16" version="1">MHQTKSNYIGWTVEDWKQFHAVSTSSESLHLLSELLLSQNSAPVDPAWISLTTSENLAHQWKFLQSKSNKSELPLYGVPVAIKDNINVKGLKTTAACPSFAFSPEEDATVVKLLREAGAIIIGKTNLDQFATGLVGTRSPYGITPNTFDSKYVSGGSSAGSASVVARGIVPLSLGTDTAGSGRVPAALNNIIGLKPTKGVFSCSGVIPACKSLDCVSVFSLNLKDAQLAFSVLAREDGDNDEYSRSFPESPLLKFPTTPKVAVPTGLLWYGETENPRLFSKAVDIFQSKVGADLVSLNIDPLLDLAKCLYEGAWVSERFAATKDFLATNPSDLDPTVEQIIKGGEKYSAATAFEFEYKRQGILQKVKKLLKDIDVIIVPTAPLTPTIKQVTDEPIKVNSYQGTYTNFVNLADMSALAIPGDFRSDGLPFGITLLSHKFNDYALLELASRYLKSYGPRFYGCLKNQIVSPENDEISVSIPNHISEVEVKLAVVGAHLKGFQLHWQLESVDARFIESTTTSSNYKLYSLPKTGPIAKPGLRRVAEGGSEIIVEVYSIPKDKFGKFISYVPEPLGIGSVELKSGEWVKSFICEEYGYTLPGTVDITEHKGWANYHSWLDSQTSKPFNSVLVANRGEIAVRIIKTLKKLGIKSIAVYSDPDKYAQHSLLADVAIPLCGTSTGETYISIDKIIQAAKDSGAEAIIPGYGFLSENADFSDRCGQEGIVFVGPSGNAMKQLGLKHSARKIALEAGVPLVPGSDGLVKDANEAKEIAAKLEYPVMVKSTAGGGGIGLQKVDCEEDIERVFQTVQNQGQSYFGDSGVFLERFVENARHVEVQMLGDGRGNAIAIGDRDCSLQRRNQKIIEETPAPNLPEATRIKMWEAARSLAASMNYKCAGTVEFIYDEIRDEFYFLEVNARLQVEHPITEMVTGIDLVEWMLLIAADTPPDFNQTVEIKGASMEARLYAENPVKDFMPSPGQLTEVKFPQWARVDTWVEKGTVVSAEYDPTLAKIIVHGKDRNDALRKLRQALDETVVFGCITNVDYLRSIANSQLFENAEVHTKVLDSYDYKPQAIEVINPGAYTTIQDFPGRRGYWNIGVPPSGCMDQYAFRIANRIVGNHHAAPGVEITLNGPTLLFHQETVVAVTGGQAEVQVNNVTVNQWEPISIKSGDKLSIGKLTTGCRAYLSIRGGIEVTEYLGSRSTFALGNLGGYNGRVLKFGDVLFLGQPEVSSCTLPAPISQPTTVPKSLIPNYANKTWQVGVTCGPHGSPDFFKPESVAEFFSSSWKVHYNSNRFGVRLIGPKPKWARPDGGDAGLHPSNTHDYVYSMGAINFTGDEPVILTCDGPSLGGFVCEAVVIESELWKIGQVKPGEFIQFVPVSYDTAKTMKSHQDSIIEDLQGELPSFEGNVLKAPENPVLSQLKISDNGPKVTYRQAGDRYILVEYGDNSLDLKLSYRIHKLVEMVKQYKTVGIYEMSPGVRSVLVEFTDDITQAQLLETLIAYEQEILFVDNYEVKSRIIRLPMAFEDQKTLDAVTRYQETIRSEAPWLPNNVDFIAAINGITRYDVKDMLYSARFLVLGLGDVFLGAPCAVPLDPRQRFLGTKYNPSRTYTPNGTVGIGGNYMCIYTMESPGGYQLVGRTVPIWDKLSLGQHSGDEKPWLLNIFDQVEFYPVSEEQVDEFTEEMNAGKFKVDIIDTVFNHGEYLEWVKEHSQSIEEFQKQQGGEKLEEFNKLIQISNSELEKTDSKIEPVEEFDESSEMIYSEYSGRFWKSLVEVGVEVVANQALIVVEAMKTEMVVNAPRDGKVVKIYPKNGDMVEAGDLVVVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFS7</accession>
    <name>G3AFS7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Obg-like ATPase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">OLA1</name>
        <name evidence="6" type="ORF">SPAPADRAFT_58206</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372478.1">
        <property type="nucleotide sequence ID" value="XM_007372416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFS7"/>
    <dbReference type="FunCoup" id="G3AFS7">
        <property type="interactions" value="1035"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFS7"/>
    <dbReference type="GeneID" id="18872366"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58206"/>
    <dbReference type="eggNOG" id="KOG1491">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018395_1_2_1"/>
    <dbReference type="InParanoid" id="G3AFS7"/>
    <dbReference type="OMA" id="VLRCFDN"/>
    <dbReference type="OrthoDB" id="424823at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04867">
        <property type="entry name" value="TGS_YchF_OLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01900">
        <property type="entry name" value="YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.300:FF:000001">
        <property type="entry name" value="Ribosome-binding ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000001">
        <property type="entry name" value="Ribosome-binding ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.300">
        <property type="entry name" value="TGS-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00944">
        <property type="entry name" value="YchF_OLA1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004396">
        <property type="entry name" value="ATPase_YchF/OLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023192">
        <property type="entry name" value="TGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013029">
        <property type="entry name" value="YchF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041706">
        <property type="entry name" value="YchF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00092">
        <property type="entry name" value="redox-regulated ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF11">
        <property type="entry name" value="OBG-LIKE ATPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06071">
        <property type="entry name" value="YchF-GTPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006641">
        <property type="entry name" value="CHP00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="5">
        <location>
            <begin position="22"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="31"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00059898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="396" mass="44389" checksum="E8D2D75FA2216D4B" modified="2011-11-16" version="1">MPPKKKGAQEKPILLGRPGNNLKSGIVGLANVGKSTFFQAITRCPLGNPANYPFATIEPEEARVIVPSPRFDKLVELYKPKSEVPAFMTVYDIAGLTKGAHAGEGLGNNFLANIRAVDAIFQMVRCFDDADIIHINDEVNPVADLDIIKDELRLKDIEFAQKHLEGIEKITKRGGQSLEVKQKKEEAEFVKKIIAMLEEGKRLANQTWTQKEVEIINTMLLLTAKPCIYLINLSERDYIRKKNKHLLKIKEWVDTNSPGDLIIPISVSLEEKLANMETDDERDAYCKEIGAVSALPKIIVAMRQKLDLISFFTGGPDEVREWTIRRWYTAPQAAGTIHTDLERTFILAQVMKYEDLVELGDEASVKAGGKLYTKGKDYFVEDGDILYIKAGEGKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFZ3</accession>
    <name>G3AFZ3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58321</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372544.1">
        <property type="nucleotide sequence ID" value="XM_007372482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFZ3"/>
    <dbReference type="GeneID" id="18872399"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58321"/>
    <dbReference type="eggNOG" id="ENOG502RPY6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1028807_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFZ3"/>
    <dbReference type="OrthoDB" id="4018768at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="271" mass="31142" checksum="4A58522895A79975" modified="2011-11-16" version="1" fragment="single">MQDNNHGPTTSPASSLASSPIIKPRRAKSLDEIDADNIFKEFDCDQDMLRFNRLKHFISLNHLPSKQEEHDSDDSTNIDYSNYDMVSVCSDMSYYSPEKQENGDDEDRISFDDFSKFLRNSRVELNKDIREAFPHLIAPPHTSTKFHNPSENVHRVAPSLSTDSKDVETSIRAEHQQDPKELLNRVISYSQLPMTPPAGLSYQRRYSMSSPTPKHSRKSQQFTSPRRPPLKHSNNKTKPHSYSSPTRLPKQKSYSSPTRFLKHSPPRDKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG03</accession>
    <name>G3AG03_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multidrug-resistance transporte</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_131326</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372554.1">
        <property type="nucleotide sequence ID" value="XM_007372492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG03"/>
    <dbReference type="FunCoup" id="G3AG03">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="GeneID" id="18869564"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131326"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_22_1_1"/>
    <dbReference type="InParanoid" id="G3AG03"/>
    <dbReference type="OMA" id="YKMDENA"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17502">
        <property type="entry name" value="MFS_Azr1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF198">
        <property type="entry name" value="AZOLE RESISTANCE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="49"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="305"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="525"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="52"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="557"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="557"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="571"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="602"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="611" mass="66078" checksum="58A2A554FF24F7C6" modified="2011-11-16" version="1">MNRDEIATANSTSAAVDLEGQDNPHKKFKEADAGDGTKREDQFLHGTHLVLCFISLFLCLFIFALDQTIVVTILTTVGNKFNDFSNVAWLSSGFLLTMAVFIQPFGKLSIIFGRRWTMVAAVVIFEAGSLMCALANNMNVLIGGRVLAGIGGSGIQGLAFVIITEIVPINKRPVGMAIVAVTFAVSSVLGPLIGGAFTSHVSWRWAFYINLPIGGIAMAFFLYSFRPPTPKGNYIKQLKEYDYLGTFLLISGSVLFLLALTFGASDFSWDSGAVISCFILGPVLLILFCIWNFRFSKNQIIPTDVVIVPQIIASVLALAGIFSAFIAALIYLSVYFQVVKDHSAMGAGLHLLPCIIAVVLCSIFSGVMIQKFRYVKVFNVVSGVVGVIGTGILALLKVDSSFGTQVGLLIPLGIATGLQMQPSIMSAQIKAPKTPGGLIMTTIFITFCRSLLAAIAGDLADAVFNTSLRNIYSKAIQEPANASVAQQLQNVQLDSLISSNSVLSTLSPEATLFIKEQMVKSMRNVYYMCIGFAVISFVASLFVTNKNVPKNVDMGEKVEQEEKFEEKTSEPRTSSGSDENISVQSDIDPSDDIEINSISKNVTREKNVEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG04</accession>
    <name>G3AG04_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multidrug-resistance transporte</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_58330</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372555.1">
        <property type="nucleotide sequence ID" value="XM_007372493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG04"/>
    <dbReference type="FunCoup" id="G3AG04">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="GeneID" id="18872406"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58330"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_22_1_1"/>
    <dbReference type="InParanoid" id="G3AG04"/>
    <dbReference type="OMA" id="VWKPEQA"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17502">
        <property type="entry name" value="MFS_Azr1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF198">
        <property type="entry name" value="AZOLE RESISTANCE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="299"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="396"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="519"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="55"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="547"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="547"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="566"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="580"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="602"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="612" mass="66470" checksum="83A93E406AE1923B" modified="2011-11-16" version="1">MSREELAAVNSATSENAPADLEGQDHHHKKFKEADAGDGTKREDQFLHGTQLVLCFISLFLCLFIFALDQTIVVTILTTVGNKFNDFSNVAWLSSGFLLTMAVFIQPFGKLSIIFGRRWTMVAAVVIFEAGSLMCALANDMNVLIGGRVLAGVGGAGIQGLAFVIITEVVPINKRPIGMAILAITFAVASVLGPLIGGAFTSHVSWRWAFYINLPIGGIAMAFFLYSFRPPTPKGNYIKQLKEYDYIVLFLLALTFGASEFPWNSGAVISCFVLGPVLLILFCVWNFRFSKNQIIATDIVTVPQIVASVLAISGIFSAFIAGLIYLSIYFQVIKDHSAMGAGLHLLPCIISVVLCSIFSGVMMQKFRYVKVFNISSGIIGVIGTSILLLLKVDSSFGTQVGLLIPLGMAAGLQMQPSIMSAQIKAPKTPGGLIMTTIFINFSRSLMAAVAGDLSDAVFNTSLRNIYSKAIRDPANAAVAQQLVHIDLNSLISSNAVLNSLSPEASYFVKDQMVKSMKNVWYMCIGFAIISFFASLFVTNKRVPKHVEMGKKDKPEDEKAIEEENEERSGDSSRDSVSDENASQQSDEASGPSEETINSISKDVTREKDIELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG91</accession>
    <name>G3AG91_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Monocarboxylate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_58435</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372642.1">
        <property type="nucleotide sequence ID" value="XM_007372580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG91"/>
    <dbReference type="FunCoup" id="G3AG91">
        <property type="interactions" value="147"/>
    </dbReference>
    <dbReference type="GeneID" id="18872449"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58435"/>
    <dbReference type="eggNOG" id="KOG2504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_1_2_1"/>
    <dbReference type="InParanoid" id="G3AG91"/>
    <dbReference type="OMA" id="AWCNGSI"/>
    <dbReference type="OrthoDB" id="6499973at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF315">
        <property type="entry name" value="TRANSPORTER MCH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="59"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="353"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="490" mass="53423" checksum="5D950670650B298F" modified="2011-11-16" version="1">MESIKSKNSGELGCTPSKVQTREAVGLAESSSEEGVLELGNKEELVNEPAIDPNVDGGYAWVICFACFLFNFSTWGMNSGFAIYFANYLNNDTFKGADKMDYSYIGGIAFGVGLFFSPVITIIQGKIGARGCLIIGNCLEFTALMLASFSKHLWQLYLTQGLIQSFGLAFMSVPATSLLPQYFKKRRVLAGSLATAGSGVGGIVFNLGMQKVVEARSVFWALRVQSIIGFVMAWVAIALTRDKQKVKIQFTMFDIGVLKSAAFYLLSFFVVTCMFGYVIVLYTLAQFATSLGYTEQQGSIVSAMVQVGSCFGRPIVGFLCDKHGPVTVTTFCYYIVGIFCLAMWIPARNLATIIVFAIVQGSMMGAIYGMMAQLIARLFGLKKMNVVLSNVWCLLGVAGVFSPVIGVKLKRGGGGFVDPTQYVNCSIFTGCSFIACATTLLIIRGYIKARDQILHESEDHKDSDYGDYTGVSVPIRKVFTHIFSNDLQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG95</accession>
    <name>G3AG95_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein transport protein SEC31</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Protein transport protein sec31</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_48258</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">The COPII coat is composed of at least 5 proteins: the SEC23/24 complex, the SEC13/31 complex, and the protein SAR1. SEC13 and SEC31 make a 2:2 tetramer that forms the edge element of the COPII outer coat. The tetramer self-assembles in multiple copies to form the complete polyhedral cage. Interacts (via WD 8) with SEC13.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPII-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat SEC31 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372646.1">
        <property type="nucleotide sequence ID" value="XM_007372584.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AG95">
        <property type="interactions" value="740"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG95"/>
    <dbReference type="GeneID" id="18871200"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48258"/>
    <dbReference type="eggNOG" id="KOG0307">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003033_2_0_1"/>
    <dbReference type="InParanoid" id="G3AG95"/>
    <dbReference type="OMA" id="WLERPCG"/>
    <dbReference type="OrthoDB" id="542917at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000526">
        <property type="entry name" value="Protein transport protein SEC31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.940.10">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040251">
        <property type="entry name" value="SEC31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923">
        <property type="entry name" value="SEC31-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923:SF11">
        <property type="entry name" value="SECRETORY 31, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="7 16" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="113"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="253"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="779"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="806"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="859"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="806"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="899"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="921"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="950"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="993"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="1035"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="1061"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="1092"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1228"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1249"/>
            <end position="1263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW35234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1368" mass="146164" checksum="5A3BA040B598CD02" modified="2011-11-16" version="1">MKIDEIARTSTFAWSPDNLPLLATGTVAGTLDINFSSSITLELWDIFSSTNSKSPIFSAPVENRFYALAWSKPFEGRPQGLLAGAFENGLVEFWDADILIKTKDLAQASVYKSQKHTGAVKSLQFNPIQNHVLVSGGSHGQIFVWDTKTFAEPIAPGQAMSPMDEVTCVAWNNSVSNILASTGNGGYTSIWDLKHKKELLHLSYSGPNGKANFSHVAWHPTISTKLITASDNDSCPLLLTWDLRNANEPEQILEGHKKGVLSLDWCKQDPELLVSSGKDNTTILWNPVKGIKLGEYPTSSNWAFETRFAPAAPDIFATASFDGKIIVQSIQDTSPPVAAKVNTNEDDFWNDLSTVDIHQPVIEVRQAPQWLKKTSGATFGFGSKLVTLKTGPDGKSIVSIEKFISGGQETSIKLFDDLKADNYTSIIEAKLTSEAVNPHDRADWEVLNKLSQTGRESLFKNEIESQPVAPVQESLLDLKTENGDDDFFAHLGNGPTMTKRDSTFVPSGNFKIFSATENESNKKLISLILANKIDEAISTCLDQDRLVEASILALDASEKAKEKVRSAYFTSKGDDNVCRIIYNASSKNVTDLVAHADVENWKEIAVAISSFSSDTDEYNNKMTELGNRILHAGAGDSQRDQAILCFLAGNALDQIAHIWLKELPEYESELLLSGTSGVSSPSDARLQAVTNFVEKIATYRHLTGATGEITGPMVEPVSKVVLEFANLVAGSGEFELANKFLQILPSEFAGTEKERISKATSTTVSAARVAPVNNVDVSSARGAPSRLTSSYAPAVSSGYPTTSAYNQAPAQPYTQPTYNQPSSYAPRQPSVASAVPHVNPYARAGNPYIPPAPHVANPAAHLSPLSNPVGINAPPPPPPKTGVKNDTEGWNDLPDTFKPKAALGRAAAAATPVPVQPAPHQPAAAPTSAPTARRSVSTQSLAPPPRKGSRSASSKVLPSATFSPKPPHVVTNRYAPPPGAELGSPASTTNGFSAPPSNSSPAKKNPYAPTGEVNPVKISYAPPAPTRLASAPVAPMAPPPKNPYAPPPQASPLIQHSAGVAPPPKMGGMIPAPPQPHFGANTPPIQPAFNNVPPPPRQHSAVAPPPQRQPSAVVPPPPRHASAVAPPPPNQQPVVAVPPPQQQQPQPQPAHNPYAAPPPPPPQQPVATPPPPPPQAQKQFAPPPPQHAAPPPSLHPQPAAVPPPQPQLQPQPQPQIAAPPPVVPVAPSPQVNNVAPVQQAPAAAPEPVEPEKIKHAPGDRSHIPESSLPIYESLNKVLVAIKPNIPAKYARHGNDMEQRLNMLFDHLNNDDLSSGAIESLRQVSTSLEAREFEKATAFSVEIATNYGGEIGNWHIGLKRLITMAEAMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGA1</accession>
    <name>G3AGA1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58451</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372653.1">
        <property type="nucleotide sequence ID" value="XM_007372591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGA1"/>
    <dbReference type="GeneID" id="18872454"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58451"/>
    <dbReference type="eggNOG" id="ENOG502TE7V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2793931_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGA1"/>
    <dbReference type="OMA" id="CGCADSY"/>
    <dbReference type="OrthoDB" id="3358017at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="69" mass="7781" checksum="81A86B67004E58B7" modified="2011-11-16" version="1">METSRLSHFLENTYIPPQKPTRAAVVPSYHPCLIVQDRQTTDVTKIKPCGCADSYCVKNAPAWIPRAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGC9</accession>
    <name>G3AGC9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peroxin/Ferlin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_146330</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372680.1">
        <property type="nucleotide sequence ID" value="XM_007372618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGC9"/>
    <dbReference type="STRING" id="619300.G3AGC9"/>
    <dbReference type="GeneID" id="18870712"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_146330"/>
    <dbReference type="eggNOG" id="ENOG502S05F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016397_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGC9"/>
    <dbReference type="OMA" id="GFSRYTR"/>
    <dbReference type="OrthoDB" id="5586090at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006614">
        <property type="entry name" value="Peroxin/Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052646">
        <property type="entry name" value="Peroxisomal_PEX28-32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010482">
        <property type="entry name" value="TECPR1-like_DysF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31679">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31679:SF2">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06398">
        <property type="entry name" value="Pex24p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00693">
        <property type="entry name" value="DysFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Peroxin/Ferlin" evidence="6">
        <location>
            <begin position="275"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="406" mass="47950" checksum="1FF8585F529BAD96" modified="2011-11-16" version="1">MTSEKIHHWNMPEFNKKLRASFEPSDTLLLTTTPTRQLLADSPALVSALSQIFPYLLLIDNFLEIITWTNEDHYQNFLVIVAYACTVLYWSWISYLILPMLMVLVFTSIVWSISSVIYDSKYDEKPTIEEVLYTLHNITVRFEMLFRPFKHIPLTRNNFVKLYIVTIMLTPLHFLLIRTILTPQKYLCMFGICLFTFHSPWAFATRRLFWRSLYVRIAAFYLTGADIKITKTHEQLPSQPASAATSDVEESAAAIPVLGDFRIIKKSIVSPTRVKQIVKFEILENERRWIGVGWSKVLYPHERSNFCYDKSLNTAPNIKDTSINEDNFPFPVFENDLYKYRWEWVDTDWKLDEEFNKSKTENGWVYYDTKWSDGRFRDGFSRYTRSRKWTRKAALLIDKQGTVYDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGI2</accession>
    <name>G3AGI2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Carboxypeptidase</fullName>
            <ecNumber evidence="12">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_48326</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in degradation of small peptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Release of a C-terminal amino acid with broad specificity.</text>
            <dbReference type="EC" id="3.4.16.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="12"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372733.1">
        <property type="nucleotide sequence ID" value="XM_007372671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGI2"/>
    <dbReference type="FunCoup" id="G3AGI2">
        <property type="interactions" value="782"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGI2"/>
    <dbReference type="MEROPS" id="S10.A49"/>
    <dbReference type="GeneID" id="18871204"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48326"/>
    <dbReference type="eggNOG" id="KOG1282">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008523_10_4_1"/>
    <dbReference type="InParanoid" id="G3AGI2"/>
    <dbReference type="OMA" id="TSCDDTV"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046938">
        <property type="term" value="P:phytochelatin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.410:FF:000001">
        <property type="entry name" value="Carboxypeptidase Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF113">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE CTSA-4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 12" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005131586" description="Carboxypeptidase" evidence="12">
        <location>
            <begin position="23"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW35321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="535" mass="60322" checksum="9E5E95429605A095" modified="2011-11-16" version="1">MKANHLLTGLVALIYSSSGVNASWFPNSHQVILQNEEQTNDIFNFNLAGVPAEVGEAWQEMKETLSQSAIAKLIKEYQNPMSSISNSRLKLGGMNEFEALSHDKFSDYSLRIKKNHPEILGLDRVNQYTGYLDIESLDKHFFYWFFESRNDPANDPIILWLNGGPGCSSSTGLFFELGPSSINSTLQPVHNPYSWNSNASIIFLDQPVGVGYSYTGGDEVKNTATAAKDVFVFLELFFQKFPSYLTNKFHIAGESYAGHYIPKFASEILSHADRSFELSSVLIGNGITDPLIQSASYKPMGCGEGGYKSVLTEEQCEQLDKDYPKCARLTKLCYKFPTALTCVPAQYYCDVKLFKPYSDTGLNPYDIRKPCVEEGGNCYIEMNYMDDYLNLDYVKQAVGASNIDIYTSCDDKVFRNFILDGDESKPHQQYVAELLEKNIPVLIYAGDKDYICNWVGNFAWVNELDYSGKEKFSSKPLQKWYPQGVDGKAAGEVKNHKHFTFLRIYDAGHMVPFDQPENALAMVNTWIQGDYSFGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGQ7</accession>
    <name>G3AGQ7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58608</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372803.1">
        <property type="nucleotide sequence ID" value="XM_007372741.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGQ7"/>
    <dbReference type="STRING" id="619300.G3AGQ7"/>
    <dbReference type="GeneID" id="18872530"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58608"/>
    <dbReference type="eggNOG" id="ENOG502S2YE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_53_2_1"/>
    <dbReference type="InParanoid" id="G3AGQ7"/>
    <dbReference type="OrthoDB" id="3364175at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046464">
        <property type="term" value="P:acylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050266">
        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798:SF5">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE ABHD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="213" mass="23248" checksum="6BE095EB2B29A31D" modified="2011-11-16" version="1" fragment="single">HTVLLLDHEGANKSKLTKNDLTLADLAQNVSDTLHELSLQTKKIILIGHSMSGMLVNYINIHSNLPIYKSVLIGPVHPTPKLTQIFLDRITSLKESQSLIPFSNTIPEAATGDKCSDLKKAFIRQLIQGNTVDGYIANCHAIASGASYDLDYSKVKSPTLVLYGTQDNTAPYTGCVEVIANGIPDVKAVPLDVGHWLVIEDDESVLKEIVEFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGS1</accession>
    <name>G3AGS1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Isopentenyl-diphosphate Delta-isomerase</fullName>
            <ecNumber evidence="4">5.3.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Isopentenyl pyrophosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Isopentenyl-diphosphate delta-isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_58621</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>isopentenyl diphosphate = dimethylallyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23284"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="5.3.3.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:23285"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the IPP isomerase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="4"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372816.1">
        <property type="nucleotide sequence ID" value="XM_007372754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGS1"/>
    <dbReference type="FunCoup" id="G3AGS1">
        <property type="interactions" value="795"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGS1"/>
    <dbReference type="GeneID" id="18872537"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58621"/>
    <dbReference type="eggNOG" id="KOG0142">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060552_0_2_1"/>
    <dbReference type="InParanoid" id="G3AGS1"/>
    <dbReference type="OMA" id="KAPFDNG"/>
    <dbReference type="OrthoDB" id="510307at2759"/>
    <dbReference type="UniPathway" id="UPA00059">
        <property type="reaction ID" value="UER00104"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050992">
        <property type="term" value="P:dimethylallyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045337">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009240">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02885">
        <property type="entry name" value="NUDIX_IPP_Isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000012">
        <property type="entry name" value="Isopentenyl-diphosphate Delta-isomerase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011876">
        <property type="entry name" value="IsopentenylPP_isomerase_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02150">
        <property type="entry name" value="IPP_isom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885:SF0">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0752">Steroid biosynthesis</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="16">
        <location>
            <begin position="96"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00083206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW35404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="289" mass="33006" checksum="19C9C94BBADA2A35" modified="2011-11-16" version="1">MTTSSYAELVASLSSEDIRSQWPEITPLKKISGIPRSSGSEITSVSSRDTEIFDGHDEEQIRLMEELCIVLDNDDKPVGAGTKKLCHLMENINGGLLHRAFSVFLFNEDGKLLLQQRADEKITFPAMWTNTCCSHPLCVPKELGVDDDSDSGAINRLDVAIKGAKFAAQRKLDHELGIAYADTPVENFTYLTRIHYKSASGDESSRWGEHEIDYILILKTKNDIKINANYNEVKDYKYVDAQELKDMFQDKSLVFTPWFKLICQDFLFKWWGNLDNLEQFQDDKIHRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHE4</accession>
    <name>G3AHE4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Antioxidant and copper/iron homeostasis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_54300</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ATX1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373693.1">
        <property type="nucleotide sequence ID" value="XM_007373631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHE4"/>
    <dbReference type="FunCoup" id="G3AHE4">
        <property type="interactions" value="583"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHE4"/>
    <dbReference type="GeneID" id="18871848"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54300"/>
    <dbReference type="eggNOG" id="KOG1603">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134973_3_1_1"/>
    <dbReference type="InParanoid" id="G3AHE4"/>
    <dbReference type="OMA" id="MTHTYKF"/>
    <dbReference type="OrthoDB" id="534815at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016531">
        <property type="term" value="F:copper chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006825">
        <property type="term" value="P:copper ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000008">
        <property type="entry name" value="Copper transport protein ATOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051881">
        <property type="entry name" value="Copper_transport_ATOX1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46365">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46365:SF1">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0187">Copper transport</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="HMA" evidence="8">
        <location>
            <begin position="3"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="74" mass="7999" checksum="A131512AF24966A5" modified="2011-11-16" version="1">MSGHLYHFDVAMSCSGCSGAVERVLNRLDGVSKIDISLDKQTVDVATVDSVNYDTVYNTIAKTGKKINNGNVVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHK2</accession>
    <name>G3AHK2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ion transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_148664</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373750.1">
        <property type="nucleotide sequence ID" value="XM_007373688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHK2"/>
    <dbReference type="STRING" id="619300.G3AHK2"/>
    <dbReference type="GeneID" id="18870794"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_148664"/>
    <dbReference type="eggNOG" id="KOG0255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008455_13_3_1"/>
    <dbReference type="InParanoid" id="G3AHK2"/>
    <dbReference type="OMA" id="IMFFFME"/>
    <dbReference type="OrthoDB" id="5215911at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF34">
        <property type="entry name" value="PROTEIN HOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="317"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="361"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="405"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="432"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="470"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="499"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="63"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="549" mass="61606" checksum="70C9A317CD1DA9D0" modified="2011-11-16" version="1">MSDQEKQYTIDQEVVPGTIRLIDVLGNLNVKKNDGDHNIILQPQPTSNINDPLRWTDAKKHFQFFLVWIWCFMFAVTVNFYGPLFDVWVDELNTDFNELGISVAICFLGLGLGVLIVQPTALKVGKRFTYLFCTLLGIAGCVLGAKATSIENIYVYQALGGLGAAPVDSLAEITGTDLYFQHQRSTAFGLVILALYAGSYLGPVACGYVVDGLGWRWCFWIQVVIYAIIFVLFSFTLEETSFRRDLNAEDEFEEEILSQSKDKKKENIKSTSKEVDPIDRDSVDESVPKMTYKQRMRIIHTEYNDVRSWLCIFWRPFFLVSFPAVIWGGLVYGAQMMWLSLIGNSQGDIYGAEPYNFSANGIGLTNLGAACGCITGMFYGGNFVDWSTIKLAERNNGIMEPEFRLYSMVVPTVINAAGLLAYGLGADYGTHWAVSVVIGQGCLGFAMGSTGSICLTYAVDCYHKLASECIVLILFIRNMIGMIFCFVFIPWYVNLGLRVTTWMMFALSIGINGSFIILVKWGKDYRKWTAVRYAKYSNPMFGEIFKDKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHY7</accession>
    <name>G3AHY7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TRAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_70432</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373885.1">
        <property type="nucleotide sequence ID" value="XM_007373823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHY7"/>
    <dbReference type="STRING" id="619300.G3AHY7"/>
    <dbReference type="GeneID" id="18875327"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70432"/>
    <dbReference type="eggNOG" id="KOG2187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014689_3_2_1"/>
    <dbReference type="InParanoid" id="G3AHY7"/>
    <dbReference type="OMA" id="HEQGNFR"/>
    <dbReference type="OrthoDB" id="10250660at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030697">
        <property type="term" value="F:tRNA (uracil(54)-C5)-methyltransferase activity, S-adenosyl methionine-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030390">
        <property type="entry name" value="MeTrfase_TrmA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030391">
        <property type="entry name" value="MeTrfase_TrmA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025795">
        <property type="entry name" value="tRNA_(uracil-5-)_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010280">
        <property type="entry name" value="U5_MeTrfase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061">
        <property type="entry name" value="RNA M5U METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061:SF30">
        <property type="entry name" value="TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05958">
        <property type="entry name" value="tRNA_U5-meth_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51687">
        <property type="entry name" value="SAM_MT_RNA_M5U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51622">
        <property type="entry name" value="SAM_MT_RNA_M5U_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01230">
        <property type="entry name" value="TRMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01231">
        <property type="entry name" value="TRMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="5">
        <location>
            <position position="474"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="474"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="373"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="523" mass="60393" checksum="6F24F292FDD16696" modified="2011-11-16" version="1">MFALARISLVRGTRLVNIKRGKGTKRRKDLDDPTHPNNLLQLEIQEFAKQMGVATANILNWDDFIVHEFFEMKGKNIEVNTMIHGMTTSGVGVALVPKCDYTVGFNGDLTDKYTVVFVPKTYTGDEVTITVKKHFEMHAEGEVLKVHNLGKNPLRNNDLIKCRHFETCNGCQLQMVPHEEQLNFKQTIIQRAYNYFYPEIFNSWEDEKTKFGRVISSPLQYGYRTKITPHSAYYKGRDNLVIGFQSVNSFETADIEQCPISSLEINKKYAERREEFINDYLAMRQIDKHVARVGHYFSFRNSIHIDRNTRQIQQIAVEGQQKIITERVEKYYYQFDASSFFQTNSSILPLVLKYIRTHIKSTKAPIDNLVDTYCGVGFFGIALNKDVKKKVLGIELGSKMIEFAKHNVKLNKLDAERIQFTDGDASNIFQNQHLEDMKGQNNMVIVDPSRTGSNKQFMTQLLEFEPELIVYVSCNVFTQARDLEMFEKLQGKSNVKYRVKDVVGFDFFPQTKHVETVAILEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHZ2</accession>
    <name>G3AHZ2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_148983</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373889.1">
        <property type="nucleotide sequence ID" value="XM_007373827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHZ2"/>
    <dbReference type="STRING" id="619300.G3AHZ2"/>
    <dbReference type="GeneID" id="18870806"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_148983"/>
    <dbReference type="eggNOG" id="KOG0778">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_609703_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHZ2"/>
    <dbReference type="OMA" id="LVPINIM"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.130:FF:000008">
        <property type="entry name" value="Ubiquitin-like-specific protease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.130">
        <property type="entry name" value="Ubiquitin-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF141">
        <property type="entry name" value="GH15225P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="5">
        <location>
            <begin position="318"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="511" mass="58770" checksum="08A1C9A03F5ED960" modified="2011-11-16" version="1">MSYDSDIENPLAYSRRSYGRYHNNSLVTKNPVNPVKLEPPTNLVTNSQSIVPHNIFQFIFNYLHYIYIHVSSFLHSPPQEQEVDFVKDVSPISRSTPSENSSIEIVAVNTNIDHNSAQSPPRQQNSILLDYEEDEISTAKLSNSPHDVLQELAFNRLRQHQQINQQSNTDEPQYGTDLSIAIPRPRRTSPFKPAPTFPPNYDSTILDITMPRSSSQISSLTLNPHDAYRKSILNYYVPSKPISMTSYSIVDNFISNFYIDRISSIYQSAQDKLQDVITAKRLDFLSAVKPLTSDQLTHVYNAWKSTNPHQLFVSNFQIEITTKDLQTLQDGRWLNDNIIDYYLNLIMKDYPKVFAWTTHFYSNLETKGYKGVERWGKRKKLNPFEKDMILVPVNISSTHWALTVIDNVKATITYYDSLDSQSIGNTAAVTNLNHYMNMEANRVGHAPVEYTLHPHHKQTPQQKNGYDCGVFTCTAAKFIASREGLRFGQRDMKIIRRRMTHEIINNHLLTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI25</accession>
    <name>G3AI25_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_149073</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373923.1">
        <property type="nucleotide sequence ID" value="XM_007373861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI25"/>
    <dbReference type="FunCoup" id="G3AI25">
        <property type="interactions" value="905"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI25"/>
    <dbReference type="GeneID" id="18870812"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_149073"/>
    <dbReference type="eggNOG" id="KOG0717">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009539_2_1_1"/>
    <dbReference type="InParanoid" id="G3AI25"/>
    <dbReference type="OMA" id="RANHEES"/>
    <dbReference type="OrthoDB" id="5894at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000046">
        <property type="entry name" value="dnaJ homolog subfamily C member 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051964">
        <property type="entry name" value="Chaperone_stress_response"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054076">
        <property type="entry name" value="ZUO1-like_ZHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44029">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44029:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21884">
        <property type="entry name" value="ZUO1-like_ZHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="6">
        <location>
            <begin position="4"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="504"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="408"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="523"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="411"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="432"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="477"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="489"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="542" mass="63400" checksum="6B3E0AF1489E429D" modified="2011-11-16" version="1">MKTCYYELLGVEQTATELELKKAYRRKALQLHPDKNPDNVEEANHQFSLISAAYEVLSDPQERSWYDSHKSSILNDEDEDVVDTGDSHIPSISADEIYRFFNPSLFSQVDDSINGFYVVASRLFERLAREEIQHGKYANVPGYANFRDDDNNVNALDPSLLKFPLFGNSKSSYVHYVRKFYNVWSGFSTVKSFAWRDEYRYSSAPDRRTRRLMERENKRLRDVARKEYNEVVKKFVAFIKKRDPRVKSGQEEFEKQRKKKQMEEYRNQIKQSALDKLRSTGEFQEQDWQKLTADELEDIEELLKQEYEASSDSEFDDFEDGEEEDEEIYECIICDKFFKNEQQFEIHEASKKHKKAVRQMQYEMRQEGIELGIDKDDIDLDEFETASSGFSEDDIDDELEIEDIEDIEDIENIKEEAKEVEQEPSPVKLDNFEVDDEINSDDEAESKQPKIDADLARLMEHTKLDDDDDDDWSDNKKKSKKQKKKSKGGSKEATPKPDTKLVAEKCSVCNETFDSRNKLFAHVKAEGHAAPPPKSKKKKSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI48</accession>
    <name>G3AI48_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sulfhydryl oxidase</fullName>
            <ecNumber evidence="8">1.8.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_59785</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2</text>
            <dbReference type="Rhea" id="RHEA:17357"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16520"/>
            <dbReference type="ChEBI" id="CHEBI:17412"/>
            <dbReference type="EC" id="1.8.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.8.3.2" evidence="8"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373946.1">
        <property type="nucleotide sequence ID" value="XM_007373884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI48"/>
    <dbReference type="FunCoup" id="G3AI48">
        <property type="interactions" value="449"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI48"/>
    <dbReference type="GeneID" id="18873075"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59785"/>
    <dbReference type="eggNOG" id="KOG3355">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070631_1_0_1"/>
    <dbReference type="InParanoid" id="G3AI48"/>
    <dbReference type="OMA" id="PCRTCNT"/>
    <dbReference type="OrthoDB" id="17199at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016971">
        <property type="term" value="F:flavin-dependent sulfhydryl oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160203">
        <property type="term" value="P:mitochondrial disulfide relay system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.310:FF:000003">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.310">
        <property type="entry name" value="ERV/ALR sulfhydryl oxidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039799">
        <property type="entry name" value="ALR/ERV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036774">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017905">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645">
        <property type="entry name" value="ALR/ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645:SF0">
        <property type="entry name" value="FAD-LINKED SULFHYDRYL OXIDASE ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04777">
        <property type="entry name" value="Evr1_Alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69000">
        <property type="entry name" value="FAD-dependent thiol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51324">
        <property type="entry name" value="ERV_ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8" id="KW-0274">FAD</keyword>
    <keyword evidence="3 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERV/ALR sulfhydryl oxidase" evidence="9">
        <location>
            <begin position="83"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU371123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="190" mass="21649" checksum="F389EC5C89210474" modified="2011-11-16" version="1">MSTVIEVEKTKTEVNSDAKPNFGVTGRKIVYDKDGKPCRTCNTLQDFQFATAGAKKQVKTPVVSGMNPEQPVNTKIGSYAKEDPPDVTVLGRSSWTLLHSIAATYPEEPTNKQQKDMKQFINLFAGFYPCWFCADDFQKYITTNEPKVETQDSLGRWLCDAHNDVNKKLGKPKFNCDLWKQRWKDGWDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AID8</accession>
    <name>G3AID8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Isocitrate dehydrogenase [NADP]</fullName>
            <ecNumber evidence="11">1.1.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">IDP2</name>
        <name evidence="17" type="ORF">SPAPADRAFT_59841</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19629"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="11 14">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="11 14">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="11"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373992.1">
        <property type="nucleotide sequence ID" value="XM_007373930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AID8"/>
    <dbReference type="FunCoup" id="G3AID8">
        <property type="interactions" value="812"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AID8"/>
    <dbReference type="GeneID" id="18873099"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59841"/>
    <dbReference type="eggNOG" id="KOG1526">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023296_1_1_1"/>
    <dbReference type="InParanoid" id="G3AID8"/>
    <dbReference type="OMA" id="EYPVYNF"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006739">
        <property type="term" value="P:NADP+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000002">
        <property type="entry name" value="Isocitrate dehydrogenase [NADP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004790">
        <property type="entry name" value="Isocitrate_DH_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00127">
        <property type="entry name" value="nadp_idh_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006156">
        <property type="entry name" value="PRK08299.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822:SF21">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NADP], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822">
        <property type="entry name" value="NADP-SPECIFIC ISOCITRATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000108">
        <property type="entry name" value="IDH_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="6 11 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 11 14" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 11 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11 15" id="KW-0521">NADP</keyword>
    <keyword evidence="8 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="16">
        <location>
            <begin position="12"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="78"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="97"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="312"/>
            <end position="317"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="12">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="12">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW34408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="411" mass="46212" checksum="2CDAC76E28F58836" modified="2011-11-16" version="1">MTEFKKIHVKNPVVEMDGDEQTRIIWQFIKDKLILPYLDIDLKYYDLGIEYRDQTNDQVTVDAAEAILKYQVGVKCATITPDEARVKEFGLKKMWLSPNGTIRNILGGTVFREPIVIENIPRIVPAWEKPIIIGRHAFGDQYKATDIVVPGAGELKLVFKPKDGGEIVEYPVYNFDAPGVGLAMYNTDKSITDFAESSFALAIDRKLNLFSSTKNTILKKYDGRFKDIFEDLYATKYKAKMDELGIWYEHRLIDDMVAQMLKSKGGYIIAMKNYDGDVQSDIVAQGFGSLGLMTSVLMTPDGKAFEAEAAHGTVTRHYRQHQQGKETSTNSIASIYAWTRGLIQRGKLDNTPEVTKFANDLEAAVIDTVAKDNIMTKDLALTQGKTDRSSYVTTEEFIDAVAARLNKNLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIJ3</accession>
    <name>G3AIJ3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_59074</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373292.1">
        <property type="nucleotide sequence ID" value="XM_007373230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIJ3"/>
    <dbReference type="GeneID" id="18872758"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59074"/>
    <dbReference type="HOGENOM" id="CLU_3070172_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIJ3"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="53" mass="6494" checksum="660FA4B873FE4E66" modified="2011-11-16" version="1">MVSVYPWGHLRILFKKIPNQANAQRPTRKDLYHKPYCLNPKNDVIQRLYFKRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ21</accession>
    <name>G3AJ21_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">tRNA:m(4)X modification enzyme TRM13</fullName>
            <ecNumber evidence="3 15">2.1.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_70629</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 15">tRNA methylase which 2'-O-methylates cytidine(4) in tRNA(Pro) and tRNA(Gly)(GCC), and adenosine(4) in tRNA(His).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>adenosine(4) in tRNA(His) + S-adenosyl-L-methionine = 2'-O-methyladenosine(4) in tRNA(His) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43196"/>
            <dbReference type="Rhea" id="RHEA-COMP:10401"/>
            <dbReference type="Rhea" id="RHEA-COMP:10402"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74477"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>cytidine(4) in tRNA(Gly)(GCC) + S-adenosyl-L-methionine = 2'-O-methylcytidine(4) in tRNA(Gly)(GCC) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43192"/>
            <dbReference type="Rhea" id="RHEA-COMP:10399"/>
            <dbReference type="Rhea" id="RHEA-COMP:10400"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 15">
            <text>cytidine(4) in tRNA(Pro) + S-adenosyl-L-methionine = 2'-O-methylcytidine(4) in tRNA(Pro) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32767"/>
            <dbReference type="Rhea" id="RHEA-COMP:10397"/>
            <dbReference type="Rhea" id="RHEA-COMP:10398"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the methyltransferase TRM13 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.225" evidence="3 15"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374116.1">
        <property type="nucleotide sequence ID" value="XM_007374054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ21"/>
    <dbReference type="FunCoup" id="G3AJ21">
        <property type="interactions" value="468"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ21"/>
    <dbReference type="GeneID" id="18875338"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70629"/>
    <dbReference type="eggNOG" id="KOG2811">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027610_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ21"/>
    <dbReference type="OMA" id="HRCSWRS"/>
    <dbReference type="OrthoDB" id="258806at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106050">
        <property type="term" value="F:tRNA 2'-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007871">
        <property type="entry name" value="Methyltransferase_TRM13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039044">
        <property type="entry name" value="Trm13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022776">
        <property type="entry name" value="TRM13/UPF0224_CHHC_Znf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021721">
        <property type="entry name" value="Znf_CCCH-type_TRM13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12998">
        <property type="entry name" value="TRNA:M(4)X MODIFICATION ENZYME TRM13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12998:SF0">
        <property type="entry name" value="TRNA:M(4)X MODIFICATION ENZYME TRM13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05206">
        <property type="entry name" value="TRM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11722">
        <property type="entry name" value="zf-TRM13_CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05253">
        <property type="entry name" value="zf-U11-48K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51800">
        <property type="entry name" value="ZF_CHHC_U11_48K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 15" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="11 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHHC U11-48K-type" evidence="16">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW34532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="408" mass="47177" checksum="0999A4DFBACF72BA" modified="2011-11-16" version="1">MSLFKNKKRKIEKQPKERLQCEYFLEKKNRRCGMTRKKDQRFCSEHMIHDNNQERVPCPLDPNHTVWTKDLQSHLKKCNARPKHDPWYEQDLNVSLKNRTEEQFDHEDLAEDGLFDKYIPILKSLEYGELPLKVSSHQGLTSRMEIASRQKHATQQASLIGNLKSMNLLNPDCFYVEFGCGKAELSRFVNSCILEDIKSEDATSDNYGFGLIDRGVNRLKQDNRILNDCQDKLKPTIKRTRIDIKDLDLDKFISDVSLTKIVSISKHLCGAATDLTLKSLLNSSIFENGKFGGVLIAMCCRHVCSYDQLLPQSRQYLSEHGFKTVESFNVLKKLVSWAVNTKEEETDNKYGINPEDRESVGLLARRLIDESRKYALERVLGDKYNVEIFKYIDRDTTLENVALAIVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ57</accession>
    <name>G3AJ57_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cystathionine gamma-synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_59175</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373398.1">
        <property type="nucleotide sequence ID" value="XM_007373336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ57"/>
    <dbReference type="FunCoup" id="G3AJ57">
        <property type="interactions" value="178"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ57"/>
    <dbReference type="GeneID" id="18872807"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59175"/>
    <dbReference type="eggNOG" id="KOG0053">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_3_0_1"/>
    <dbReference type="InParanoid" id="G3AJ57"/>
    <dbReference type="OMA" id="DWTKQLF"/>
    <dbReference type="OrthoDB" id="3512640at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000072">
        <property type="entry name" value="Putative cystathionine beta-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF35">
        <property type="entry name" value="CYSTATHIONINE GAMMA-SYNTHASE (AFU_ORTHOLOGUE AFUA_7G01590)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW33814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="390" mass="42509" checksum="7934C23747A412E4" modified="2011-11-16" version="1">MHLSTKSIHAGDSLKRVSDVIPPINISTTFSYSSNPDELVAIGDLEGTIAGSSQYIYSRENHPNAAIVEKTIESITGAHAITYNSGLGAFNGLITLVNPKTLVIGQAYHGCHGIADIWERNYGLKKLDIDCSDEEFDKLVKPGDLVHLETPVNPYGVNLEIAKYAKRAHAKGAILSVDATFAPPPLMDPFKFGADIVMHSATKFFGGHSDLLAGILLTKSLETKKKLLSDRLVLGTNIGNLESALLVRSLRTFELRIMRQSKSAEFIVKYLNENKSKFADLDIIYHGSLQTDAYIKEQMPLGQSPVFAIELKSEDAAKRLPSKLKLFHHATSLGGAESLIEWRALTDAHISRRLLRVSIGLEDPRDLLADLVGGLGGDDDLVGKIAELSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJK4</accession>
    <name>G3AJK4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-chaperonin molecular chaperone ATPase</fullName>
            <ecNumber evidence="2">3.6.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Hsp70 chaperone Ssb</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SSB1</name>
        <name evidence="13" type="ORF">SPAPADRAFT_134244</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the heat shock protein 70 family. Ssb-type Hsp70 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.10" evidence="2"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373491.1">
        <property type="nucleotide sequence ID" value="XM_007373429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJK4"/>
    <dbReference type="FunCoup" id="G3AJK4">
        <property type="interactions" value="1725"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJK4"/>
    <dbReference type="GeneID" id="18869795"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134244"/>
    <dbReference type="eggNOG" id="KOG0101">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_0_1_1"/>
    <dbReference type="InParanoid" id="G3AJK4"/>
    <dbReference type="OMA" id="NIPPMQA"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd24093">
        <property type="entry name" value="ASKHA_NBD_HSP70_Ssb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000014">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000004">
        <property type="entry name" value="Heat shock protein SSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000005">
        <property type="entry name" value="Heat shock protein ssb1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW33907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="613" mass="66498" checksum="49095A1072553747" modified="2011-11-16" version="1">MAEGVFPGAIGIDLGTTYSCVATYDSAVEIIANEQGNRVTPSFVAFTNEERLIGDAAKNQAALNPKNTVFDAKRLIGRAFDDESVQKDIKSWPFKVVESNGNPLIEVEYLEETKTFSPQEISAMVLTKMKEIAEAKIGKKVEKAVITVPAYFNDAQRQATKDAGAISGLNVLRIINEPTAAAIAYGLGAGKSEKERHVLIFDLGGGTFDVSLLNITGGVFTVKATAGDTHLGGQDFDTNLLEHFKKEFQKKTGLDISEDARALRRLRTACERAKRSLSSGTQTTVEIDSLFDGEDFSANITRARFEDINSALFKSTLEPVEQVLKDAKIAKNQVDEVVLVGGSTRIPKVQKLLSDFFDGKQLEKSINPDEAVAYGAAVQGAILTGQSTSEDTKDLLLLDVIPLSLGVAMQGNVFAPVVPRNTTVPTIKRRTFTTVADHQTTVQFPVYQGERVNCSENTLLGEFDLKNIPPMQAGEPVLEAIFEVDANGILKVTAVEKSTGRSANITISNSIGRLSSEEIEKMISDAEKFKSSDDAFAKRHEQKQKLEAYVASVESTVTDPVLSAKLKKSAKDKIEAALSDALQTLEIEESSADDFRKAELALKRAVTKGMATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJL1</accession>
    <name>G3AJL1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S20</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_59286</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373496.1">
        <property type="nucleotide sequence ID" value="XM_007373434.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJL1"/>
    <dbReference type="FunCoup" id="G3AJL1">
        <property type="interactions" value="861"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJL1"/>
    <dbReference type="GeneID" id="18872853"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59286"/>
    <dbReference type="eggNOG" id="KOG0900">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122625_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJL1"/>
    <dbReference type="OMA" id="IHKRVIH"/>
    <dbReference type="OrthoDB" id="10248551at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.600:FF:000004">
        <property type="entry name" value="30S ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_uS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_uS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018268">
        <property type="entry name" value="Ribosomal_uS10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005729">
        <property type="entry name" value="Ribosomal_uS10_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01046">
        <property type="entry name" value="uS10_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00361">
        <property type="entry name" value="RIBOSOMAL_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS10" evidence="6">
        <location>
            <begin position="22"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="119" mass="13368" checksum="3E283047B4844E3C" modified="2011-11-16" version="1">MSAPISKEKVEVEQQQQIHKIRMTLTSTKVKQLENVSANIIKNAVDHNIVKKGPVRMPTKVLKITTRKTPNGEGSKTWDCYEMRIHKRVIDLQAPADIVKKITQITIEPGVDVEVTIAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJL3</accession>
    <name>G3AJL3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59288</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373499.1">
        <property type="nucleotide sequence ID" value="XM_007373437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJL3"/>
    <dbReference type="FunCoup" id="G3AJL3">
        <property type="interactions" value="272"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJL3"/>
    <dbReference type="GeneID" id="18872855"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59288"/>
    <dbReference type="eggNOG" id="KOG3133">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043063_1_1_1"/>
    <dbReference type="InParanoid" id="G3AJL3"/>
    <dbReference type="OMA" id="YEPMKEM"/>
    <dbReference type="OrthoDB" id="27435at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033328">
        <property type="term" value="F:peroxisome membrane targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045046">
        <property type="term" value="P:protein import into peroxisome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.900">
        <property type="entry name" value="Pex19, mPTS binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006708">
        <property type="entry name" value="Pex19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038322">
        <property type="entry name" value="Pex19_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12774">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12774:SF2">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04614">
        <property type="entry name" value="Pex19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="318" mass="34643" checksum="B98ABD4FFE91DAE6" modified="2011-11-16" version="1">MSDQTQATTEVNPIKPEAAAAKEATVSKAAVPAVADDDVDDLDDLLDDFADDVLSKPPGAGVAQSPKTNPTTAGTSADAPLNDELQSGIADLIKDLNITDPETQTQFEQLVSQFETEHRAEVEAQERKPANFEHVMKETMERLKNSGKDIDDKISGDGAGIGGEDLLTQMLAGLGGDGDNLDMNKLLVEMLEQLSSREVLYEPIKDLNTKFPDYLKENKDKLDEAKYTTYNKQYEITTEIITIFEAADYSDDNKAKRDQVNELLESLQELGQPPEELVGNGEFLPGFGGGGAGNDLDFNDKDLPEDLAKDLEQGCKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK07</accession>
    <name>G3AK07_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RFX-type winged-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_70246</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373642.1">
        <property type="nucleotide sequence ID" value="XM_007373580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK07"/>
    <dbReference type="STRING" id="619300.G3AK07"/>
    <dbReference type="GeneID" id="18875314"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70246"/>
    <dbReference type="eggNOG" id="KOG3712">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011526_1_0_1"/>
    <dbReference type="InParanoid" id="G3AK07"/>
    <dbReference type="OMA" id="MIWLREN"/>
    <dbReference type="OrthoDB" id="10056949at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000422">
        <property type="entry name" value="DNA-binding protein RFX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003150">
        <property type="entry name" value="DNA-bd_RFX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039779">
        <property type="entry name" value="RFX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057321">
        <property type="entry name" value="RFX1-4/6/8-like_BCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12619">
        <property type="entry name" value="RFX TRANSCRIPTION FACTOR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12619:SF5">
        <property type="entry name" value="TRANSCRIPTION FACTOR RFX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25340">
        <property type="entry name" value="BCD_RFX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02257">
        <property type="entry name" value="RFX_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51526">
        <property type="entry name" value="RFX_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RFX-type winged-helix" evidence="3">
        <location>
            <begin position="243"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="337"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="739"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="17"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="44"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="99"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="124"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="337"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="371"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="743"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="833" mass="93633" checksum="CD257A2001830634" modified="2011-11-16" version="1">MDQNQHSHHQLDDSYKLRQNQPQQLSRQPSPNIHIQTKEHSHIQQVNPQYRQEQQRQQYHGVQYTAPPQQQYQYDYPHSYPPPPQHPPRPQLVAAQSQPVPPQSQPPPPPPQQQRFVPGPSSAPAFQSFNINQPTLPNIEQQHQHQQQISPIETSITSPLDRSKTSPSPQKRKKYKKHDYTSPADQGETELKQLAMNAADIPLAELALKIRQVENEGSTIITPGSSDHFKAKENKERQRQLFGMVWLLNSCEHSPTAVVPRNRIYARYVQVCADNSLSPLSPASFGKLVRILFPTLTTRRLGMRGQSKYHYCGIKLNGDQTFQSHLLTQQASSSSQQQQLMSGFSGQQVDSPISSSNSSLSYDSSPSSSSQIHTPSYTPINSPSIAMTPTVTDQLPSVSHMKYVPNLFKLLNSNAAPSSNPYTPIQLPSIYPYLPRDTDYDIADTLHSLYKLHVNSIFEALRFMQLKKLFASFSNFNSKLTAPAFKLYTNECIIDWVKQCDLLMYKKMIRMLNKLQLQFLIPTEQLRQLKQISNGYIKTLSGTLMNGKISRNFVIMKLKLAKHFVNLLNRLIKVIETGQPASRILTDASEKHSMVQDWNKLDIRELISREIPCGDQNIETLTYILKQEVVDLLVDKGESGPTMFDFAEYISGLPGRFPQINARLFLLLSSNLLTACLRDISLAGGEGFGAWWIVRCWTDEYLLLTVELGGFFSDDFQAMEAINLPVPVPSFASERIDAGPVGVNSQGSPTESQQIETQHNQRSFPGDGSSINEASNPVSGAGNTSGFDNSLGVIDLLDTSLEFETRNSQLASMPSQSEILVNYESNIESILNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK26</accession>
    <name>G3AK26_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vacuolar protein sorting-associated protein 28</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ESCRT-I complex subunit VPS28</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_150125</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the ESCRT-I complex (endosomal sorting complex required for transport I), a regulator of vesicular trafficking process.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the VPS28 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374351.1">
        <property type="nucleotide sequence ID" value="XM_007374289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK26"/>
    <dbReference type="FunCoup" id="G3AK26">
        <property type="interactions" value="605"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK26"/>
    <dbReference type="GeneID" id="18870856"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_150125"/>
    <dbReference type="eggNOG" id="KOG3284">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076417_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK26"/>
    <dbReference type="OMA" id="CDEFPTV"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037202">
        <property type="entry name" value="ESCRT_assembly_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007143">
        <property type="entry name" value="Vps28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017899">
        <property type="entry name" value="VPS28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037206">
        <property type="entry name" value="VPS28_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017898">
        <property type="entry name" value="VPS28_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038358">
        <property type="entry name" value="VPS28_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12937">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 28, ISOFORM 2 VPS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12937:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 28 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03997">
        <property type="entry name" value="VPS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017535">
        <property type="entry name" value="VPS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140111">
        <property type="entry name" value="Endosomal sorting complex assembly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140427">
        <property type="entry name" value="VPS28 C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51310">
        <property type="entry name" value="VPS28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51313">
        <property type="entry name" value="VPS28_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0967">Endosome</keyword>
    <keyword evidence="4 5 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VPS28 N-terminal" evidence="9">
        <location>
            <begin position="20"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="VPS28 C-terminal" evidence="8">
        <location>
            <begin position="175"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR017535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="275" mass="30663" checksum="DB23254102D6E83A" modified="2011-11-16" version="1">MNNSKSPPEYAPTATTSFTVSSSSPRYNSEITKSTLLKTSLHKTVYDSLAEIYSILPTLEMLEVSYIKDYITDKEKYTSTSYRLINQYQIILKGFVEDSDKLNLLKTILPGLDHTLSNFLQLLSNKFHCNCTHAIKRLQSGIPATIEHLSTQVESLHPTVSQNTATTPAAPNANTSAKLISQITGNFITCMDAVKLNYKTKSQLHPLLSELVVNLNDLVENEQHKSLEFSGKSKLVNWLIKINNLKDGEEIGGTDADSFLNDLDTAYKGFYTSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKJ1</accession>
    <name>G3AKJ1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA polymerase epsilon subunit D</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">DNA polymerase II subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60934</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375111.1">
        <property type="nucleotide sequence ID" value="XM_007375049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKJ1"/>
    <dbReference type="FunCoup" id="G3AKJ1">
        <property type="interactions" value="220"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKJ1"/>
    <dbReference type="GeneID" id="18873642"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60934"/>
    <dbReference type="eggNOG" id="KOG0870">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087036_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKJ1"/>
    <dbReference type="OMA" id="NTYRRKV"/>
    <dbReference type="OrthoDB" id="1707486at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008623">
        <property type="term" value="C:CHRAC"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22928">
        <property type="entry name" value="HFD_POLE3_DPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051377">
        <property type="entry name" value="DNA_Pol-Epsilon_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46172">
        <property type="entry name" value="DNA POLYMERASE EPSILON SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46172:SF1">
        <property type="entry name" value="DNA POLYMERASE EPSILON SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="7">
        <location>
            <begin position="30"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="121"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="154"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="199"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="215"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="231" mass="25953" checksum="234F0EA24C0E8FB2" modified="2011-11-16" version="1">MPPKGWRKNAEGQYPQPNKDTELISIDEILFPKSTIQKLAKTMIADDNMLLAKDSSLALQRSATVFVSHLLFHARAISKQNGRKTVSSQDMLAAVERAEFSGFIPEIKTRLANYEEMLSVKKQQRADKSASKDTDEPSAKKIKVDEGVTAPTEAESDDDVDEEIIEDEELPDEKVEDDVDEVEEDEEDDDATGNPIELLSKEEAELQGESHESTDEVEEEEEEEEEHSDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKT6</accession>
    <name>G3AKT6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Heat shock protein SSC1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_60315</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374505.1">
        <property type="nucleotide sequence ID" value="XM_007374443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKT6"/>
    <dbReference type="FunCoup" id="G3AKT6">
        <property type="interactions" value="1673"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKT6"/>
    <dbReference type="GeneID" id="18873339"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60315"/>
    <dbReference type="eggNOG" id="KOG0102">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_2_1_1"/>
    <dbReference type="InParanoid" id="G3AKT6"/>
    <dbReference type="OMA" id="MGTDWKI"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11733">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000014">
        <property type="entry name" value="Chaperone protein DnaK HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000003">
        <property type="entry name" value="Heat shock protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000007">
        <property type="entry name" value="Heat shock protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000004">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000003">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="603"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="643" mass="69165" checksum="57B017B8B673ADB6" modified="2011-11-16" version="1">MLTARNSVKTASLRAATFVRAQSTAAPTGQVIGIDLGTTNSAVAVMEGKIPKILENSEGGRTTPSIVAFTKEGERLVGIPAKRQAVVNPENTLFATKRLIGRRYEDAEVQRDIHQVPFKIVKHNNGDAWLEARGEQYSPQQIGGFILNKMKETAEGALGKKISSAVVTCPAYFNDAQRQATKDAGKIVGLNVLRVVNEPTAAALAYGLEKNDGQVVAVFDLGGGTFDISILDIGAGVFEVKSTNGDTHLGGEDFDIALVRHIVEAFKKESGIDLQKDRMAIQRIREAAEKAKIELSSTVSTEINLPFITADASGPKHINQKISRAQFEALVEPLIKKTVDPCKKALKDAGLSTSDISEVILVGGMSRMPKVIETVKSIFGREPSKAVNPDEAVAMGAAIQGGILAGEVKDVVLLDVTPLSLGIETMGGVFARLISRNTTIPAKKSQIFSTASAGQTSVEIRVFQGERELTRDNKLIGNFTLSGIPPAPKGVPQIEVTFDIDTDGIIKVSARDKATNKDASITVAGSSGLSDAEIEKMVNDAEKYAETDKARREAIESANRADQLCNDTENSLNEFKDKLSEEATTKVKQLITELREVVLKAQAGEEVSPEELKTKTEELQNESMNVFKDLYKDAGSSSEEPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKW7</accession>
    <name>G3AKW7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_60333</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374525.1">
        <property type="nucleotide sequence ID" value="XM_007374463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKW7"/>
    <dbReference type="FunCoup" id="G3AKW7">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKW7"/>
    <dbReference type="GeneID" id="18873349"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60333"/>
    <dbReference type="eggNOG" id="KOG2422">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_814211_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKW7"/>
    <dbReference type="OrthoDB" id="205993at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006994">
        <property type="entry name" value="TCF25/Rqc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684">
        <property type="entry name" value="NULP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684:SF0">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT TCF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04910">
        <property type="entry name" value="Tcf25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="385" mass="44994" checksum="A45325071805FADC" modified="2011-11-16" version="1">MTPDPESLMQLLQQYPYHVETLLQVSMVLLRQGDNKSASNALVERALFTFDRSFHKHFHEILSQGNTGLIRLPYESYMNRQFYLCLFRYIIALGERSTFFTAFSFCKFLLSLSPAEDPLGVRYFLDFYAIMSEEYKYLIQFAESPLCTTYVKWFTPGIAFSTVLAHLKLDDREKAKLALRKAYDSHPYAAFRLYQDVCLAHDPQIESQFKNLPHETVLSSETYLIRAGVLWKEHSDRQFLHDELQELFKTRKFKTESIGSGIKSSLYGLFGISNEKQEQEDIPFNLIRFAVLSGENKIMAKLPKEFWLKDDTFEYDILPPKAEYVHYNEFSGIMQDKPRITDCLLDYVDQNLLGTIIQTRTADDNDFEGMLRQLQQQDLQEEHNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKX7</accession>
    <name>G3AKX7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">Mediator complex subunit 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MED4</name>
        <name evidence="11" type="ORF">SPAPADRAFT_136188</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374536.1">
        <property type="nucleotide sequence ID" value="XM_007374474.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKX7"/>
    <dbReference type="FunCoup" id="G3AKX7">
        <property type="interactions" value="254"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKX7"/>
    <dbReference type="GeneID" id="18869946"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136188"/>
    <dbReference type="eggNOG" id="ENOG502RXM0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071875_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKX7"/>
    <dbReference type="OMA" id="PFQIHPN"/>
    <dbReference type="OrthoDB" id="1323at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019258">
        <property type="entry name" value="Mediator_Med4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13208">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13208:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10018">
        <property type="entry name" value="Med4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="243"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="131"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="253"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="311" mass="35749" checksum="5957E80031DAE91F" modified="2011-11-16" version="1">MLPYRKQDNQLKPIPRISSSQRLNQPPIQPNGTPPQQAPSTPSAYVSSSLNPIRNLPTTQGNIKSKIRTKEDLQKFEKLPIVAKVNEFETLLNGIADDISKFKDVEIQEKVKRIIEVNDILKSNIEDLNKHRDFSYQVDQLSNENKQLEERAKGILRELIFYRNELKSLPRLPKNEVTPASEVEVQEILKYAMKLAKFTKAPPAMANMPFQIHPNNYIWPAEDALRRGMLAMSSLQGDEIIKQELGGEEEEEEVKKETVDEVMKEETIPKQEQEEIIPRRGSFGYGTTTKKKEEQTDLNLDLFDPDDEYSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL13</accession>
    <name>G3AL13_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60373</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374570.1">
        <property type="nucleotide sequence ID" value="XM_007374508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL13"/>
    <dbReference type="GeneID" id="18873372"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60373"/>
    <dbReference type="eggNOG" id="ENOG502S6M4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1023659_0_0_1"/>
    <dbReference type="InParanoid" id="G3AL13"/>
    <dbReference type="OrthoDB" id="3993678at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="188"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="272" mass="31179" checksum="1485627CDF845FEA" modified="2011-11-16" version="1">MGIMTCNNELLSELELVTTELALSIKRELALETKLRGGNATTQDPSLAQLQKELLSNRKLIAELQEKLSKERRLRFISEEHALLSEHGQSPSPLKLNYENTELYKQLLIKNDEVKQLEDKIVEYEKGQKSDTDLLDKYNELLAENTDLKFHVIPNLQRQIEQRDDGNSSRVFNITGDQSFELSDEDELSTLRNQRDELRSTVNRLTRNNDVELRSAQEKIRSLEVQLRDMKRINDKLSIREDTKPATNGFIKNGGGKLSGLAIISPTQNLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL96</accession>
    <name>G3AL96_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_60447</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374654.1">
        <property type="nucleotide sequence ID" value="XM_007374592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL96"/>
    <dbReference type="FunCoup" id="G3AL96">
        <property type="interactions" value="175"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL96"/>
    <dbReference type="GeneID" id="18873416"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60447"/>
    <dbReference type="eggNOG" id="KOG0938">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026996_5_2_1"/>
    <dbReference type="InParanoid" id="G3AL96"/>
    <dbReference type="OMA" id="VWKIPRI"/>
    <dbReference type="OrthoDB" id="10259133at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09251">
        <property type="entry name" value="AP-2_Mu2_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000002">
        <property type="entry name" value="AP-2 complex subunit mu, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043512">
        <property type="entry name" value="Mu2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00314">
        <property type="entry name" value="CLATHRINADPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0168">Coated pit</keyword>
    <keyword evidence="2" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="9">
        <location>
            <begin position="201"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="167"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="463" mass="52884" checksum="4E156BDF0327D4A4" modified="2011-11-16" version="1">MISAIFLYDSKGDILISKLFKDDVKRTISDVFRIQVITQTRSRDSSTKSPVLTLGSTSFIYIKSGSVWICAVTRSNQDCASILEYLYKLELLLRSVTMDKRGSADVLTDEAIINNFNLVYEIIDESCDFGFPTNLDLSYLKNFISCLNDSDKVFKMMRKPTLKNPELSRTSSSLIPSNSAGSPPPLASNITWRSQGLKYRRNEIYLNVTEKVNVLMNQQSEILRSYVDGSIQMKTHLSGMPSCKFGFNANTVLVNYKPNSGDDYGQDRGFVVLEDSKFHQCVDLRTFENDRVIQFTPPDGEFQLMSYNCHSSINLPFRIYPQVQEIGRNRLMYKIVIKSFFPVKLPATNVVVKIPTPKTVTSKLIQHSTGKAKYHPEEHVILWKFNKFFGSQEQVLTAEVELSGESDELLYWARPPITLDFVLDMFSCSGLTVKFLRVQEKSNYKTLKWVKYTSQAGSYEVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALF2</accession>
    <name>G3ALF2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcriptional regulator of multiple amino acid permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60526</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374710.1">
        <property type="nucleotide sequence ID" value="XM_007374648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALF2"/>
    <dbReference type="FunCoup" id="G3ALF2">
        <property type="interactions" value="139"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALF2"/>
    <dbReference type="GeneID" id="18873448"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60526"/>
    <dbReference type="eggNOG" id="KOG1286">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_8_6_1"/>
    <dbReference type="InParanoid" id="G3ALF2"/>
    <dbReference type="OMA" id="FIRFYYG"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF46">
        <property type="entry name" value="SPS-SENSOR COMPONENT SSY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="344"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="7">
        <location>
            <begin position="1"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="518" mass="57410" checksum="E6029C083D74DD17" modified="2011-11-16" version="1">MVTFVSVVDGVSGLSSRFVDDAFGFAAGWLYFFCFAFGVAGEIVAGVIMLSFFPNLQTTKSQGSAAGFVTFFFVAILGSNLLDIRIFGEIEYMSSLIKVLFISVMMIVMIILNRGGMGGDVLGFRYWTHSKSDFENNIIFGLFRPTFNLHDTGTSSPTEGIGGDLGRFMSFLVALLVVAYAYSGTEIVCIAACEAKNPRKTLPSATKRVFWRILLFYVLATFLVSLNIYAGDPRLLRYIRPKMAVEDFQSNPAINAVGGIHCNFDPTYFAGYPSGAQSPWIVALQSVGQCRFGSVVNAFWVFFAVSCGNAQLYVSSRTVYSLSLQGKAPRFLSYCNRYGTPYNAVLLAGSFGLFAYSCVSESATVVFQNLSTITASSVIFIWFAMCLTYIRFYYGLKRRPDILSRDDESYPFKSPFQPYTALVGLVGTGSIIFASGYTVFLTGEWNTLFFFSSYGTLIAFAILYLAYRLIKGTSIPSLDRLDFDAGRTEMDRYIWDAGRDYNVRSFKDVAQKILSFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALN1</accession>
    <name>G3ALN1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">rRNA adenine N(6)-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_136286</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Mitochondrial transcription factor that confers selective promoter recognition on the core subunit of the yeast mitochondrial RNA polymerase. Interacts with DNA in a non-specific manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374789.1">
        <property type="nucleotide sequence ID" value="XM_007374727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALN1"/>
    <dbReference type="FunCoup" id="G3ALN1">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="GeneID" id="18869955"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136286"/>
    <dbReference type="eggNOG" id="ENOG502QY7G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034228_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALN1"/>
    <dbReference type="OMA" id="WDYVTKH"/>
    <dbReference type="OrthoDB" id="16079at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034245">
        <property type="term" value="C:mitochondrial DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034246">
        <property type="term" value="F:mitochondrial transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006391">
        <property type="term" value="P:transcription initiation at mitochondrial promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="entry name" value="Ribosomal RNA adenine dimethylase-like, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF17">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="353" mass="40633" checksum="8BC71C0121C770D6" modified="2011-11-16" version="1">MSLRSFNPAFKQLFADRKPMRLHIHEMLNPHTVQQVLDGMRLKKLYPDGSKLDIIECNPGNGVFSSMVNYDLKPRNHILIESRTKLQQHWKKDLQALQGITGNKENFTLYPRDPYDWQTYLDLTDKDKIIQPVTKSYDQVHDELLLIANWCYDRGEKIVAQWIGCCGDRNWLQKYGKVRVICMAPSKTVSKFLSEPGFGKRNRTAMKRELFTDSKLLAIIANSANLPGEGYDPRVLIRDQPVAAYDNSIPKSTEMGIFEFYPSGMTTDAIAEMEYLLSPMYMNRSTPLRQILPKIVSGGEGMAESLPHDLLDRSPREFTTEDILAIAHAYDKWPFKPDYVDTIQLDEIDESQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALY6</accession>
    <name>G3ALY6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_50230</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SH3YL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374854.1">
        <property type="nucleotide sequence ID" value="XM_007374792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALY6"/>
    <dbReference type="FunCoup" id="G3ALY6">
        <property type="interactions" value="287"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALY6"/>
    <dbReference type="GeneID" id="18871420"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50230"/>
    <dbReference type="eggNOG" id="KOG1843">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015320_2_0_1"/>
    <dbReference type="InParanoid" id="G3ALY6"/>
    <dbReference type="OMA" id="SNCKARN"/>
    <dbReference type="OrthoDB" id="443981at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11842">
        <property type="entry name" value="SH3_Ysc84p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11525">
        <property type="entry name" value="SYLF_SH3YL1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000100">
        <property type="entry name" value="SH3 domain-containing YSC84-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051702">
        <property type="entry name" value="SH3_domain_YSC84-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033643">
        <property type="entry name" value="SYLF_SH3YL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007461">
        <property type="entry name" value="Ysc84_actin-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629:SF2">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING YSC84-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629">
        <property type="entry name" value="SH3YL1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04366">
        <property type="entry name" value="Ysc84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="347"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="231"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="308"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="245"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="258"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="277"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="308"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW33339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="406" mass="43679" checksum="74DB91C6B8A41E12" modified="2011-11-16" version="1">MGINNPIPRSLRSESKKAAKILASFVKPNQLAGPDQVIPPRILQNAKGLAIITVLKAGFLFSGRAGSGVIVARLPDGSWSPPSAIVTAGAGVGGQIGAELTDFVFVLNTKAAVDSFAQAGSVTLGTNVSVAVGPLGRNAEAAGTASLKSVSAVFAYSKTKGLFAGVSLEGSAIVERREANRKFYGSNCKARNILSGQIEAPPACDTLMNVLASRVFSNRFDDDYDSDYYDDIPDEFSDSTSSPRRGSRTTSRRRSTYSDEDDDYYSDEDDRYASRSKNSTRPTSRGTNTGTRKASNWEDDLYDSNYRSRDRDVDRLGSRMENTRISNSGPSRPPASSKPNFGGAPKTNTNQAVALYTFKGEQAGDLPFKKGDVIEIIKKSQTADDWWTGRNNGVTGIFPANYVELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMC9</accession>
    <name>G3AMC9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_137569</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374296.1">
        <property type="nucleotide sequence ID" value="XM_007374234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMC9"/>
    <dbReference type="GeneID" id="18870056"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137569"/>
    <dbReference type="eggNOG" id="ENOG502RQDA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1620104_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMC9"/>
    <dbReference type="OMA" id="WIITYLL"/>
    <dbReference type="OrthoDB" id="4022545at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="164" mass="19311" checksum="2018B2330FAFDA33" modified="2011-11-16" version="1">MSYFDPFEAKLERRNHATIQVTAKFFNNAIPAFNTISLSKLTKFTIQTQITLSQHQIMHILNLDSSEPLHNEVYASLINVVRKTARYLYVNTKLSTLREFPMTNVEFVSFEIARKFPELFYVPVEKSGGEFRLSYKCRFTEMWIITYLLKQEYSKVVDILYSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AME9</accession>
    <name>G3AME9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_60146</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome b5 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374316.1">
        <property type="nucleotide sequence ID" value="XM_007374254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AME9"/>
    <dbReference type="STRING" id="619300.G3AME9"/>
    <dbReference type="GeneID" id="18873241"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60146"/>
    <dbReference type="eggNOG" id="KOG0537">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1810882_0_0_1"/>
    <dbReference type="InParanoid" id="G3AME9"/>
    <dbReference type="OrthoDB" id="260519at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050668">
        <property type="entry name" value="Cytochrome_b5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359:SF95">
        <property type="entry name" value="CYTOCHROME B5 TYPE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="6">
        <location>
            <begin position="25"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="143" mass="16262" checksum="C20599473A12B8E3" modified="2011-11-16" version="1" fragment="single">MSPSLQIQTNQGNSPPMKHAKHRSVQYFTPEQVRQHDTPDDLWMIIYNKVYDVTTIAKEHPGGIEVLFDCGGEDATESFEDVGHSDYAFSLLAPGYLGEIIPAQQIQYSNPQPQIIYNTSKPDIKSDDIWNLGKPAKKNWEWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMH1</accession>
    <name>G3AMH1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Increased recombination centers protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_60166</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Is probably involved in a pathway contributing to genomic integrity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the IRC22 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374338.1">
        <property type="nucleotide sequence ID" value="XM_007374276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMH1"/>
    <dbReference type="FunCoup" id="G3AMH1">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMH1"/>
    <dbReference type="GeneID" id="18873254"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60166"/>
    <dbReference type="eggNOG" id="ENOG502S7BF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078554_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMH1"/>
    <dbReference type="OMA" id="WLPETYK"/>
    <dbReference type="OrthoDB" id="1926781at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005595">
        <property type="entry name" value="TRAP_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12924:SF0">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12924">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03896">
        <property type="entry name" value="TRAP_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442434" description="Increased recombination centers protein 22" evidence="10">
        <location>
            <begin position="20"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="199"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="25994" checksum="BEE199D1E50DA2DE" modified="2011-11-16" version="1">MKFTSVLTLFTAAIASVSGYETTSEPNAIGILADYHIVQTPNIKSNDVANWINDETVTISYQIQNNEAEEITLIGVTGSYRDPTTGEIASNLTNARIDALVIEPGTSGSFEQDIAVNLIPGNYLLVPQIFLAHENQVKVLTVRGQLLTIDDAPISIFDPRLILLELVLLASLGGLAYLGYDIWGKQYFQGVVPVKAAAATPRKGASPSSEGSSRSGTPSGSSYDVNWIPEGHLKQKKTKKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMI4</accession>
    <name>G3AMI4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_60787</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRP7 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374943.1">
        <property type="nucleotide sequence ID" value="XM_007374881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMI4"/>
    <dbReference type="FunCoup" id="G3AMI4">
        <property type="interactions" value="266"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMI4"/>
    <dbReference type="GeneID" id="18873568"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60787"/>
    <dbReference type="eggNOG" id="KOG4008">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053375_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMI4"/>
    <dbReference type="OMA" id="GIHKWIA"/>
    <dbReference type="OrthoDB" id="5390at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12293">
        <property type="entry name" value="dRRM_Rrp7p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040447">
        <property type="entry name" value="RRM_Rrp7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040446">
        <property type="entry name" value="RRP7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024326">
        <property type="entry name" value="RRP7_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13191">
        <property type="entry name" value="RIBOSOMAL RNA PROCESSING PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13191:SF0">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 7 HOMOLOG A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17799">
        <property type="entry name" value="RRM_Rrp7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12923">
        <property type="entry name" value="RRP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rrp7 RRM-like N-terminal" evidence="3">
        <location>
            <begin position="6"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA-processing protein 7 C-terminal" evidence="2">
        <location>
            <begin position="160"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="24231" checksum="B5099819A74A6DE2" modified="2011-11-16" version="1" fragment="single">MTTITEIKGYHILPITLPNAHSTHYIYFKKHDAKQATSNRSLFIFNLPISTNITTLKKYFQDVAIGATIESFTPSLLTDHPEDIWISLTKLTSDLELANGDSEEASAKLPKNCGIVTFIDKAAFQLAFNALKKLSSNSTASNWPLITFNSNYYLQKYQNQVLDIEELSEYVSQSLVEFDRAEKESMEQLQQQTQLVDEDGFTLVVGSHRKTKAGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMK2</accession>
    <name>G3AMK2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA ligase</fullName>
            <ecNumber evidence="15">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_151563</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="15"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374961.1">
        <property type="nucleotide sequence ID" value="XM_007374899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMK2"/>
    <dbReference type="FunCoup" id="G3AMK2">
        <property type="interactions" value="571"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMK2"/>
    <dbReference type="GeneID" id="18870899"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_151563"/>
    <dbReference type="eggNOG" id="KOG0966">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004844_1_1_1"/>
    <dbReference type="InParanoid" id="G3AMK2"/>
    <dbReference type="OMA" id="EGIMIKH"/>
    <dbReference type="OrthoDB" id="151490at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032807">
        <property type="term" value="C:DNA ligase IV complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097680">
        <property type="term" value="P:double-strand break repair via classical nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043007">
        <property type="term" value="P:maintenance of rDNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07903">
        <property type="entry name" value="Adenylation_DNA_ligase_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07968">
        <property type="entry name" value="OBF_DNA_ligase_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044125">
        <property type="entry name" value="Adenylation_DNA_ligase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029710">
        <property type="entry name" value="LIG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45997">
        <property type="entry name" value="DNA LIGASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45997:SF1">
        <property type="entry name" value="DNA LIGASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 15" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 15 19" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="17">
        <location>
            <begin position="396"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="18">
        <location>
            <begin position="672"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="18">
        <location>
            <begin position="825"/>
            <end position="933"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW33446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="948" mass="110961" checksum="D7581DF9C2E770B7" modified="2011-11-16" version="1">MPFFLDNVPTPKNNLEPSYRFLNDELFIKLDAVNKQTLGEFKNISDKKAYIINSFIQQFKLHIGNDIYPSARLIFPDKAGRLYYIKDIVLARLIIKMYSIPKDSDDYSMLYNWKLQYQKTKRFTVDQHKLRDLPLQAARIISNRRNYMNETHEFSVADINAKLDALNLAKKSAEQIEILKPLFDTLTIPELRWLIHIILKKTILGFFENYFLNSWHPDASRLYSICNNLQKTVNYLVDPNTRVDPNLLTVHPFYPFKPQLAQKLSSSYAKLIKDFQKITDMEPQMQTLFDKLDLKDKFYIEEKMDGDRMVLHKEGPHFKFYSRRLKDYSFLYGENYEFGSLTKYLTEAFPGNVDSVILDGEMVAYDYVRKAILPFGTLKSSAIQESVRQYTIIDQYEQQSSYPYFLVFDILYLNGRNLTNYPLFFRKNILNKVIRPIPNRFEIHDSRISSSIEDIKRAIREIVGSRSEGLVLKHTQSKYHIGYRNLKWIKLKPEYLEKFGENLDLVVIGKVPGVKNSYMTGLRNETDGSYYSFCMCANGFTNEEFDKIERITHGKWIKTKDQIPSKSVIQFGTKQPPWWINPRDSLVLEIKARSIDAQSEKTFAVGSTLHNMYCRRIRTDKNIDECISMQEYFQLKEKFSTDVLKSQSAALKRKQLHNLDLFNESTIKKQRVESNLFARFNFMIVSDKLDPITGERTSREELMKLVKKYGGNLINAVDVLTADRMLVITEKMLPLCESFINRGIDLIKPNWIFECINRGTILQLEPFFIFSTSNWDKYTVGKVDQFGDSYIIYNSITELSIPRLSDSELRGARKLHMHEWGDQRPKLYLFLDTNFFVVGDTMSAKFLGQRIMRYTGEITKDINGCSYIVVPIYDDEKNRIETLDAVNKISSKISEGLLFDEGSFTRIPFIVTEKFIDQCIKENCIVDPEDYKYYQKQISFSKKSFAIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMQ4</accession>
    <name>G3AMQ4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribose 5-phosphate isomerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_136073</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LacAB/RpiB family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375013.1">
        <property type="nucleotide sequence ID" value="XM_007374951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMQ4"/>
    <dbReference type="STRING" id="619300.G3AMQ4"/>
    <dbReference type="GeneID" id="18869940"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136073"/>
    <dbReference type="eggNOG" id="ENOG502RZN7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091396_1_0_1"/>
    <dbReference type="InParanoid" id="G3AMQ4"/>
    <dbReference type="OMA" id="VREWLTY"/>
    <dbReference type="OrthoDB" id="2106730at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1400.10:FF:000004">
        <property type="entry name" value="Ribose 5-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1400.10">
        <property type="entry name" value="Sugar-phosphate isomerase, RpiB/LacA/LacB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011860">
        <property type="entry name" value="Rib-5-P_Isoase_Actino"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003500">
        <property type="entry name" value="RpiB_LacA_LacB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036569">
        <property type="entry name" value="RpiB_LacA_LacB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051812">
        <property type="entry name" value="SPI_LacAB/RpiB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004051">
        <property type="entry name" value="PRK05571.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02133">
        <property type="entry name" value="RPI_actino"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00689">
        <property type="entry name" value="rpiB_lacA_lacB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43732:SF1">
        <property type="entry name" value="RIBOSE 5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43732">
        <property type="entry name" value="RIBOSE 5-PHOSPHATE ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02502">
        <property type="entry name" value="LacAB_rpiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005384">
        <property type="entry name" value="RpiB_LacA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89623">
        <property type="entry name" value="Ribose/Galactose isomerase RpiB/AlsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="156" mass="17028" checksum="B142639F9174B851" modified="2011-11-16" version="1">MTTQKFRIVIGCDQAGYEYKEQIKKDLTHNPNVIEIVDVGVDSNCNSTAYPLVATTAAEKVANREADRGIIICGTGLGVAISANKVKGIRAATAHDSFSVERSILSNNCQILCMGQRVIGLELARRLAQEWLSYVFDDSSSSAAKVAQIEKYEYAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMW0</accession>
    <name>G3AMW0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_66995</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375650.1">
        <property type="nucleotide sequence ID" value="XM_007375588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMW0"/>
    <dbReference type="GeneID" id="18875129"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66995"/>
    <dbReference type="eggNOG" id="ENOG502RQBI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1078125_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMW0"/>
    <dbReference type="OMA" id="WKEISKM"/>
    <dbReference type="OrthoDB" id="4022836at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="260" mass="29838" checksum="D443861DB36844ED" modified="2011-11-16" version="1">MIFFNCIFANTIQVDPLGITPNASDFNVIEVDSFANLNIADFKNIDNVFKIKGDPLGTTIVNKRRMNMLERFGGNEFKMNDLRDHIENEDDEWIEIKSFEEPIVSVGPRLPLTNCLNQRYGDGGSLEFGLGTAFLLVETVDSDRVVNLIVYYHKFKEKFELQWAVAVTLGYGCNVPKGKVGQMFITPYMAQFSDVQTRTIKIVTTRGWFRKKKRKFKIGKWKVENPVSLFDIRKSPIIQCVTDESLLDCHGKLKGKFRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMX6</accession>
    <name>G3AMX6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Selenoprotein O</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_139014</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SELO family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375666.1">
        <property type="nucleotide sequence ID" value="XM_007375604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMX6"/>
    <dbReference type="FunCoup" id="G3AMX6">
        <property type="interactions" value="341"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMX6"/>
    <dbReference type="GeneID" id="18870171"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139014"/>
    <dbReference type="eggNOG" id="KOG2542">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010245_2_1_1"/>
    <dbReference type="InParanoid" id="G3AMX6"/>
    <dbReference type="OMA" id="HVVNLNA"/>
    <dbReference type="OrthoDB" id="10254721at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070733">
        <property type="term" value="F:AMPylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00692">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003846">
        <property type="entry name" value="SelO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057:SF14">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02696">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="623" mass="70993" checksum="489E88FF67C67BBB" modified="2011-11-16" version="1">MTHTGKLLDIPKSSSFTKRIKPDNRIPTVEVALKNENGITNTPRNLLSGGFSWTLPTPRKEYEFLTASPNALRDLGLDPQQAEDPVFQSIVSGEFYEDKVTFEKEGFPMPYSQAYAGWQFGQFAGQLGDGRVVNLFEVPKVSSSSSSSSSSSNPANRDKYEIQLKGAGKTPYSRFADGKAVLRSSIREYIISEHLNAIGIPSTRALALTYLPSTIAQRHGAERCAIVARFAESWVRLGSFDLYRWRGDREGIRDLSDYVIEELFTFNNTKFANFNRIINLKTDLFNSNSNVIGELSDYDKMFYEIIVRNAETTALCQCYGFLNGVLNTDNTSVLGLTIDFGPFSIMDKYDPNYTPNSEDHERRYGYRNTPTAIWWNLTRFGEDLAELIGAGLELVNTPEFKEGVKEEWEDSIIKRATKIIEVGGDMFQYAFTKKYVETFFNRLGLAHELIDVKNPDFHQINLIAPLLEILLKLQTDFNLFFLTLQNINLDQADYVSIAENEILKDVDFNNVRHDKSSLLKEIAEWLITYQDLKVKSLAYESRPDPRNYNPLFLPRNWILDQVIAQAQESRGENITHLKKLEKMSLNPFDKSKWGDELKELENSWILQGQKGDEYAMLQCGCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN19</accession>
    <name>G3AN19_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_153357</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375709.1">
        <property type="nucleotide sequence ID" value="XM_007375647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN19"/>
    <dbReference type="STRING" id="619300.G3AN19"/>
    <dbReference type="GeneID" id="18870951"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_153357"/>
    <dbReference type="eggNOG" id="KOG1601">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_652107_0_0_1"/>
    <dbReference type="InParanoid" id="G3AN19"/>
    <dbReference type="OrthoDB" id="515401at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013860">
        <property type="entry name" value="AreA_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF281">
        <property type="entry name" value="BOX A-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08550">
        <property type="entry name" value="GATA_AreA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="10">
        <location>
            <begin position="158"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="268"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="352"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="268"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="429" mass="47793" checksum="1C57C647053A0FD1" modified="2011-11-16" version="1">MYQHKNNYLPHNQRISNIAWRMQNKKMMNQPAINNGTGGGITKPIVRSNSSSGRFGARSNSLSRVISNTSTAKKNASVASANSDNDPTLDTFDYVDHIRRISQEEYGGTSEFPDTTTMPAMGRAMSIPADSKNSSFAIAAGAKSDYKFRIKSPSPTNSKKVLQCTNCQTKTTPLWRKANNGDLLCNACGLFYKLHGVLRPLNSSLDTKEQKKMNKKKINKNDQNISNTNTNLFAGLNQAQFVNNDNLNKIPTEDVNLDSFLDFNVTANNMNNSEPAQTPVGLSSSLPDRITSQQQPQLPSQEQQQQQQQQQQQQPPPQPQQLNGANFDDFEKFLGVNLYPDFNQRQFFEMEHPMGIPPDSQLPQGSSAYPQQQPPQPQNSQYPAQPVQQAPDMTMLDPAMANNVNYNYTNNTNGNNGSWNWLDFSPATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN50</accession>
    <name>G3AN50_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_50506</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375169.1">
        <property type="nucleotide sequence ID" value="XM_007375107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN50"/>
    <dbReference type="STRING" id="619300.G3AN50"/>
    <dbReference type="GeneID" id="18871450"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50506"/>
    <dbReference type="eggNOG" id="ENOG502QWCT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_809335_0_0_1"/>
    <dbReference type="InParanoid" id="G3AN50"/>
    <dbReference type="OrthoDB" id="4050369at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="343" mass="39487" checksum="1ABD4349030D31D2" modified="2011-11-16" version="1">MYLVRTVDGVKLEEANPGGCIKVPVYKLSMQGSKYHIVYIAVPNDDSISKDNHMQVDFSTVDTSNNNSYLSGLCEGVELTAEHISLLKKLDQSPTSFNGEEFYTKVYDNVISKPTSRCYYVPKGHNSMTLSPFFNVHTSNVKKVYTRYFGYLYAILVNYDKFDFMNTAAEGKARELLESVKYADTFDDLFDYFMMENDNILNLALLPFLLDLKTHRLKATDFSLKKLNGLKSFCKRVLFLKNDQGTIHKFVAQSNFSQSDFIGWAKLVHIYQALKEERGEYVKFKVTPGPGESVYVNGTNVSPSYFNKLYDSAYDHFRKQYDSMSQLIEDYHTIEEVWGPRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANF5</accession>
    <name>G3ANF5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphatidylinositol/phosphatidylcholine transfer protein Sec14p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61059</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375220.1">
        <property type="nucleotide sequence ID" value="XM_007375158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANF5"/>
    <dbReference type="FunCoup" id="G3ANF5">
        <property type="interactions" value="305"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANF5"/>
    <dbReference type="GeneID" id="18873698"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61059"/>
    <dbReference type="eggNOG" id="KOG1471">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014001_0_1_1"/>
    <dbReference type="InParanoid" id="G3ANF5"/>
    <dbReference type="OMA" id="WAFSTVW"/>
    <dbReference type="OrthoDB" id="1434354at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120019">
        <property type="term" value="F:phosphatidylcholine transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032120">
        <property type="term" value="P:ascospore-type prospore membrane formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031322">
        <property type="term" value="P:ascospore-type prospore-specific spindle pole body remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048194">
        <property type="term" value="P:Golgi vesicle budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120010">
        <property type="term" value="P:intermembrane phospholipid transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001246">
        <property type="term" value="P:negative regulation of phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901352">
        <property type="term" value="P:negative regulation of phosphatidylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000011">
        <property type="entry name" value="SEC14 cytosolic factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051026">
        <property type="entry name" value="PI/PC_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657:SF1">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="99"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="301" mass="34892" checksum="E35088A23B2C69C5" modified="2011-11-16" version="1">MTTISEEEILASYPQVNAPTDQTGYTSNLTEEQKQLVIDLRKRLQELGYKSRLDDASMLRFLRARKFDLEKSLEMFVNCEKWREEFGVNTILQDFHYQEKPIVASMYPQYYHKTDKDGRPVYFEELGKVDLYQMLKITTQERMLKNLVWEYESMVQYRLPACSRQAGYLVETSCTILDLKGISVSSAYSVIGYVREASKIGQDYYPERMGKFYLINAPFGFATAFKLFKPFLDPVTVSKIFILSSSYQKELLKQIPPQNLPTKFGGLSQVTDQELLLNDVGPWRDPKYIGPEGEAPRSFQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANN0</accession>
    <name>G3ANN0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_67159</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375835.1">
        <property type="nucleotide sequence ID" value="XM_007375773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANN0"/>
    <dbReference type="STRING" id="619300.G3ANN0"/>
    <dbReference type="GeneID" id="18875146"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67159"/>
    <dbReference type="eggNOG" id="ENOG502S084">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_595794_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANN0"/>
    <dbReference type="OMA" id="QCERCQN"/>
    <dbReference type="OrthoDB" id="4083304at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="103"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="15"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="374" mass="43812" checksum="40EC744863177AA4" modified="2011-11-16" version="1">MIRHEFSNKLLELFHDELSELRKQDQEKIQALQVKINKLTRTITEKDQEIAQLKAQLKPTRRLSVSSFIKPDHDISPTKEEEFISPARKPQFMVSDRSILATQYSDDDEEVDTSSKKKSPFKRKLSSSQGSIIISPRKSTTSLGPPEKIPRLNATFELDEFSPVKPTQYSSSSSHSSQVHEEVEDSEDEVVEILQFPIISIPKNVTTILQKRKYLLNYYTDRFHNDIEFKINLIRHPINEINWDFADFKANPNYKQSKSLIHNHKIINQKQYNKVKAFYQAANINNDEFNDSFEDKLSQIFDKFASPPGFMNSEFPDTQEQQARRELLKKRQNKRLARRIKECTSVDRGKQIGEFMFVVDILNMYVVSDRWFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3APF4</accession>
    <name>G3APF4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GPN-loop GTPase 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_152618</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Small GTPase required for proper localization of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375407.1">
        <property type="nucleotide sequence ID" value="XM_007375345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APF4"/>
    <dbReference type="FunCoup" id="G3APF4">
        <property type="interactions" value="1060"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APF4"/>
    <dbReference type="GeneID" id="18870932"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_152618"/>
    <dbReference type="eggNOG" id="KOG1533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037460_0_2_1"/>
    <dbReference type="InParanoid" id="G3APF4"/>
    <dbReference type="OMA" id="ATHNYFL"/>
    <dbReference type="OrthoDB" id="5839at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034087">
        <property type="term" value="P:establishment of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17871">
        <property type="entry name" value="GPN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000338">
        <property type="entry name" value="GPN-loop GTPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030231">
        <property type="entry name" value="Gpn2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF3">
        <property type="entry name" value="GPN-LOOP GTPASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="305"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="305"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="320"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="348" mass="39954" checksum="C2EC630C8C8F668C" modified="2011-11-16" version="1">MFGQIVIGPPGSGKSTYCYGMYQFMSAIGRKSCIVNLDPANDRLPYPDCALDIRDFITLEDIMEELNLGPNGGLMYALESLDHEGIDMFLSKIDQLIQDKNYILFDCPGQVELFTHHNSLFKIFKRLTQTKRMRLCVVSLVDSIYLTSPSQYISILLLSLRSMLQLDLPHVNVISKIDMLRNYGELPFRLDYYTEAQDLGYLTPHLEQESNTVLGKNYVRLTELIAELVEEFHLVSFEVLSVENKKSMINLLSVIDKANGYSFGSEIGGDSVWSEATRQGGSGVYQDVDIHERWIEYKDEYDKEEHKQEEELRKQHQENDVEMTEEDEWEAALQDWESKRGGQGALSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3APL2</accession>
    <name>G3APL2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SMP-LTD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_139353</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375459.1">
        <property type="nucleotide sequence ID" value="XM_007375397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APL2"/>
    <dbReference type="FunCoup" id="G3APL2">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APL2"/>
    <dbReference type="GeneID" id="18870201"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139353"/>
    <dbReference type="eggNOG" id="KOG2238">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012852_0_0_1"/>
    <dbReference type="InParanoid" id="G3APL2"/>
    <dbReference type="OMA" id="MDKEDWY"/>
    <dbReference type="OrthoDB" id="26740at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21675">
        <property type="entry name" value="SMP_TEX2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466:SF19">
        <property type="entry name" value="NUCLEUS-VACUOLE JUNCTION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466">
        <property type="entry name" value="TEX2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15413">
        <property type="entry name" value="PH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="11">
        <location>
            <begin position="299"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="132"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="501"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="613"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="702"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="140"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="515"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="633"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="730"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="757"/>
            <end position="785"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="785" mass="87848" checksum="DE7E3929C1A97C53" modified="2011-11-16" version="1">MWLITFLSIYIFGGITFIPGLIILVLYLHPIYKNDKQDREETKPLKAGEIEESNQSGLESYKAGWIFVTRDYIDSPDQINSNTQPITESNDNKSAYSSLYKLVSDDATTSMLSNNSQSTDVDDLSINESKETLVQPAAPPASSATTTTTTTTTQQNIRSSQRRHRYYAVLKHGNLFLYKDETLKDVKHVIVLSSYFVTIYPRELSDAQFFTKYSAIALINPNKLILTNDVDTATPPKGSFFIYCDINSDKEDWYFALIRATKHNSELPDSLNPSTFAKTLHFNTKDMINLIQTLYSSEGQLQTKWLNAIIGRLFLGLNGTKVMEDYIRTKLSKKLNKIKTPGFLDKFQIKEISPGHSAPFITYPNLQEINPDGTIVVSAYVSYTGGLSVTISTKVNINLGAKFTNREVDILLRVTLNRFEGPILIKIKPQPSARVWYTYKTEPIMNINIEPVISARQVSYNIVTNYMEKKLKEAIRDSLVLPHWDDIVFFETTEELYRGGIWDPAASAPSPPETPTDQSSESTPSRSEEASIDRDLDDDVETKSELSVKLANQKMKLTNTLTDISKRMKKKASSSNVNVISSEECQTTIAVRASPALSTLKKIGKWYFKDEKQQRDESYTPPEMISNRRPPRKSSSVSTTTPPNGFDSKFSEHPSPSYDFGKLNHERDVHSLTTSSWPDNISVMSGDEVSIKSGLLVSEPTVGLTQPLTPRSHKSIRKPPSSPPPPLPEDLSKEATPKCEDVPTTIEDISIPRSEDISSPQTINGQPLVPSENNGKSSPVTNNTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3APM4</accession>
    <name>G3APM4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_50773</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375471.1">
        <property type="nucleotide sequence ID" value="XM_007375409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APM4"/>
    <dbReference type="STRING" id="619300.G3APM4"/>
    <dbReference type="GeneID" id="18871482"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50773"/>
    <dbReference type="eggNOG" id="KOG1256">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_45_4_1"/>
    <dbReference type="InParanoid" id="G3APM4"/>
    <dbReference type="OMA" id="NRGELCV"/>
    <dbReference type="OrthoDB" id="1700726at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF33">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="171"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="754" mass="85042" checksum="D66686AC3DAB9052" modified="2011-11-16" version="1">MSDSLFSAHDFSKEVAVSHLPLKPNIICDSIAVKGSNGNANYSKVFRNKAIPNRIVKTIHPDLNTHHKLFANAAELFKNKPCLGARPYDYVNKKSAPEFKYFTYGEVNEKKKRIGAGFIRSLLANPFKNSELVAHKKIDHHLRDWMNYGTKSTGRNNPDFEIEKANSFILSIFASNRMEWILSDLACSSFSITNTALYDTLGPEVTQYILELTGSPMIVCSMDKIPQLLDLKKKFPKQLENFISIVSMDPAELIPTESFELAYELRVTIQDLRQIEEIGGENPIKELAPNPDTLYTISFTSGTTGSKPKGAMIPQSMAAAYISCLLCFEPHAGPNDKAFIFLPLTHIYERETSSFALCGGYYLGFPQLTIGRKDVNSFNDLIDDLRVFKPTYFSIVPRLLTRLEALIKVKIKELNPNDQEKVNKIIHYKLQEQSKADGSTGFDFTFDNFGPYKELRQFVGFDNIKWVQTASAPVAASTLIFLKASLNIGIRQLYGLTESGGAITATDAYESNPGSCGSIGPTGEFKLRNARDMGYNISELKGELLLRGPQIFKGYYYNKAETDVVFNEEGWFHTGDIASIDGKTGRITIIDRVKNFFKLQQGEYISPEKIENRYLSSNPMITQLYVHGDSLKSYLVGIVGIEYEKGLKFLNEEFGYNKIAMSSEELLEELNKVETKSKFLHIMNKNVKDKLSGFEILHNIHIEINPLTVERDVVTPTFKIRRPVASKFFAKVFHRLYEIEQSLVLAARLKAAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3APM6</accession>
    <name>G3APM6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Geranylgeranyl pyrophosphate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_61280</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375473.1">
        <property type="nucleotide sequence ID" value="XM_007375411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APM6"/>
    <dbReference type="FunCoup" id="G3APM6">
        <property type="interactions" value="467"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APM6"/>
    <dbReference type="GeneID" id="18873814"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61280"/>
    <dbReference type="eggNOG" id="KOG0777">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_6_0_1"/>
    <dbReference type="InParanoid" id="G3APM6"/>
    <dbReference type="OMA" id="CAFLEML"/>
    <dbReference type="OrthoDB" id="6921389at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:geranylgeranyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033386">
        <property type="term" value="P:geranylgeranyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF44">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="344" mass="39482" checksum="8B014C7E8C9221FD" modified="2011-11-16" version="1">MDIDINEIIKPVSGYISRPSHEPILEPYNYLAEIPGNNQNVRTKFINAFNELYYKIDNRSLLDAIGEIISIFHNSSLLIDDIEDSSLYRRGLPAAHTKFGIPLTINCGNLMYFIALQKAQHDLPLKYFTGVADSEEVDKYKFETSQILIDEMLNLHHGQGLDIYWRDYLIKLEKLPDIEDYLQMVKDKTGGLFRLSIKLLTLYSNASLGFDLIPVANLLGIIYQLRDDYLNLVDDKYSAMKGIAGEDLIEGKLSLPILHCLRTSNESNPVYKLLHTYTTSEERKNQPELVEQSIRYMITESKSLEYTLELIHDLETKVKDLILSEAPDSEKSLLFQIINHLCDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3APP5</accession>
    <name>G3APP5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_66856</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375492.1">
        <property type="nucleotide sequence ID" value="XM_007375430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APP5"/>
    <dbReference type="GeneID" id="18875121"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66856"/>
    <dbReference type="eggNOG" id="ENOG502T4X9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1653217_0_0_1"/>
    <dbReference type="InParanoid" id="G3APP5"/>
    <dbReference type="OrthoDB" id="4024815at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="160" mass="18394" checksum="E294E2E305F4E894" modified="2011-11-16" version="1">MNSPEHPSNESISSSTKFSSPQNGDQEPETNELDFKLAVDESPGVQLYQLSNWLDFEPIMLPNVNISYGNEDFQVGYYKLLKQFNKLVLTVCQKNNELNVVKFKCKYLIDEMIKEWNEGEESEEELNHAHIKVAKSEVAEKLKDFFDPKEEFESTAGSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3APR5</accession>
    <name>G3APR5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_61317</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375512.1">
        <property type="nucleotide sequence ID" value="XM_007375450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APR5"/>
    <dbReference type="STRING" id="619300.G3APR5"/>
    <dbReference type="GeneID" id="18873838"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61317"/>
    <dbReference type="eggNOG" id="KOG0811">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059257_4_0_1"/>
    <dbReference type="InParanoid" id="G3APR5"/>
    <dbReference type="OMA" id="VQITYEP"/>
    <dbReference type="OrthoDB" id="364348at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15840">
        <property type="entry name" value="SNARE_Qa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF38">
        <property type="entry name" value="LD27581P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="4">
        <location>
            <begin position="185"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="279" mass="32677" checksum="609505983750CF5F" modified="2011-11-16" version="1">MSFNNPFDTDLESTRTNQSYKYQDFPEFDSLTTTIENQLHHINHSLISKIKEDLVSYEHGQIELGDALSKQFKKTTESFKKVNEVVKSINSVISDKERNKEDVEVIGYLRQRESIQIKLIKDALINFKNLQKRYESLTGEHQEESRSQEEQISAQQQQQQQQQQQQTQVQITYEPINAEELEQQSLLIQQRERELQQIHTDTLEINEIFTNLSSIIQEQQFQVDSIENNIFSYNTNAHQASNELHRAERYQRRSNGRMLCCLVILIGVTAFIILLGIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ18</accession>
    <name>G3AQ18_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein YTP1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61416</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375615.1">
        <property type="nucleotide sequence ID" value="XM_007375553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ18"/>
    <dbReference type="FunCoup" id="G3AQ18">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ18"/>
    <dbReference type="GeneID" id="18873882"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61416"/>
    <dbReference type="eggNOG" id="ENOG502QRB1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034579_1_0_1"/>
    <dbReference type="InParanoid" id="G3AQ18"/>
    <dbReference type="OMA" id="DYQHTAM"/>
    <dbReference type="OrthoDB" id="4137487at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018827">
        <property type="entry name" value="YTP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31685">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_6G12730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31685:SF2">
        <property type="entry name" value="PROTEIN YTP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10355">
        <property type="entry name" value="Ytp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="368"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Protein YTP1-like C-terminal" evidence="2">
        <location>
            <begin position="145"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="426" mass="47660" checksum="B6F60118C903597B" modified="2011-11-16" version="1">MAYAMEMDMDEPKVEFHPINPGSKTFHWILTLFILLIIPSVSAVFAIANRLSWSLVLHFIAAGYSVFETLFLDFPDNIDNHENRTSKGTSWFLSALLGVTVFVGTFINGTNLVINKFYPKYASPNEYSKMSKLYKSLSVVVVLTGWVRVCMAPVALFGFCYGRHTGQCIAHGIMGSAFIAYSFVLSLVLVVPWIRKHQLLSQGAGKMKSQEFLDSTVMMVWGIVNTFTEHRWGREGWSMGDYQHTSMGIIWWAGGLLGMFLSRNNNRSFVPALLLIFTGYSMSAHSQHLMISTKVHAVFGIALMGAGVTRIMEISLLLKDKACSPSGKILQFQYFPCFCLCLAGIMFMGANEEQLQLVHDLGSDHSAYVLVVSSAAFMIFLWFQGLLWGYLRLVGYDEDGEIAPAKYEAVDIEDFELGDMSAEETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ66</accession>
    <name>G3AQ66_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_61975</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376191.1">
        <property type="nucleotide sequence ID" value="XM_007376129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ66"/>
    <dbReference type="FunCoup" id="G3AQ66">
        <property type="interactions" value="177"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ66"/>
    <dbReference type="GeneID" id="18874132"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61975"/>
    <dbReference type="eggNOG" id="KOG4412">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000134_18_2_1"/>
    <dbReference type="InParanoid" id="G3AQ66"/>
    <dbReference type="OMA" id="WAVAYNR"/>
    <dbReference type="OrthoDB" id="539213at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904855">
        <property type="term" value="F:proteasome regulatory particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="111"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="144"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="178"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="232" mass="25401" checksum="279BF916F0B7851F" modified="2011-11-16" version="1">MNDSEQTNKFVIHDAIKAGNTNLAKSLIDQFPKEIYLFDDDNRTPLHWACSLNNLTIVEYILSKNQVDIDDLVDSSGWTPLHITASIGNPEIFEKLLAVEPKPDLDLATNQGTTCLHLAISKNNYDIVKKLVEAKASCKVKDKKGDTPLHRASAIGSIPTVKLLVEKGKVNINAKDNDGWTSLHHALAEGHGDVAVLLVSLGADPQIKTDAGELPIDVAVDDKVRKYFVESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQE7</accession>
    <name>G3AQE7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nucleolar protein Dnt1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_62064</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376272.1">
        <property type="nucleotide sequence ID" value="XM_007376210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQE7"/>
    <dbReference type="GeneID" id="18874176"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62064"/>
    <dbReference type="HOGENOM" id="CLU_2910568_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQE7"/>
    <dbReference type="OrthoDB" id="6365676at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018844">
        <property type="entry name" value="Dnt1-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10407">
        <property type="entry name" value="Cytokin_check_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442509" description="Nucleolar protein Dnt1-like N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleolar protein Dnt1-like N-terminal" evidence="2">
        <location>
            <begin position="29"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="62" mass="7246" checksum="5C492E8D9C8745A7" modified="2011-11-16" version="1" fragment="single">MSKWKLQVLLIPLDYTYVATAQVNTSVIKRFLHLTDPNAELAIIREEIKAKYKKLYPNDEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQT1</accession>
    <name>G3AQT1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleolar 27S pre-rRNA processing Urb2/Npa2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_155063</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376406.1">
        <property type="nucleotide sequence ID" value="XM_007376344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQT1"/>
    <dbReference type="FunCoup" id="G3AQT1">
        <property type="interactions" value="161"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQT1"/>
    <dbReference type="GeneID" id="18871011"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_155063"/>
    <dbReference type="eggNOG" id="ENOG502QTEB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008472_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQT1"/>
    <dbReference type="OMA" id="CHVNARE"/>
    <dbReference type="OrthoDB" id="160374at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052609">
        <property type="entry name" value="Ribosome_Biogenesis_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018849">
        <property type="entry name" value="Urb2/Npa2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15682">
        <property type="entry name" value="UNHEALTHY RIBOSOME BIOGENESIS PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15682:SF2">
        <property type="entry name" value="UNHEALTHY RIBOSOME BIOGENESIS PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10441">
        <property type="entry name" value="Urb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleolar 27S pre-rRNA processing Urb2/Npa2 C-terminal" evidence="1">
        <location>
            <begin position="882"/>
            <end position="1082"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1082" mass="125387" checksum="46A35E430AB4C0F7" modified="2011-11-16" version="1">MSEILSAEKATKFLRSKESVVQIIETANKILDGKLDIYLPQKEIFILTLLVDRLNDRSQFNNWKYEPCVWELLTKVWYRCKEQRNQLIQKFRIIEAVCNILDKGNEKAIQSALAGLELIYREAYVEIDENSTYMLLKSFVQAAPEKSSELLLNWNDLIRDIYNRISLKSTREISKKTYSKFFQDCVPSILSYLTTPNASDIFQDILKQEMFNEDLIEYLNSNIEILLKKDTDIPSIALLYKLVVEQLASKHMDICQQIYTIITSNKKYSTLSESLLSELASSQKTLTHEFALSVYGNEVDGKMFRDINWNMVKYLFTLDNDLAVEKSSFVLEGYSSKMNIEIFEVGKAIVTAYVNNRELIEFITVVWPQAIESDKVWKSNEFVNFVSTTISTFTSKQLLYVIEQGFKLDKQYQESVFTALTTGLITCTFQMIESVKEKFIENSQFFNSSDFWHVRFNLLCLYGKEYKVSNKIMKTVNEKYYFFTLFRFLELGVVQAIGEDDQKAFLKFLKSNSDLATTVLKRWLVILNNFFDQQFLVKLLELLFTKVKVSELNQTFFEQRKLTSALFKLIMKDLQGNMSYLDSIPIHCISRTTKKDLMNQLFHLYIKRNESTLDFIKYLLSQPSYQSELELKFENLIKLVESRSTTSFAIARIIWSNHIQQIKDNEDYVINAIKLMTKYLKKAKTAISGESELCLIILSAKVPEELESQFNNFKQVFIETCLSNLDKSDDEATVNWNLRALITVSDEVDYSKIFPKLQKVAADVKTNEMKSILFKLICHTISIDLNHAIYVLSLFTALHSEGVGNLDMELFFNRLSSDKEVYSEVLDYFVNSLDEVNESYVIIACHFFSNIDKENYSSVSIAKCMTLFIQAIKQKNNSIPLVLETLRTALVDHAWIFNQYLLEKTLVIIAYLAHSATGPDIYILTTQVTSHILLYHRFKLSTRHHILLHIATELLRPFCQDSPIASSRDAAASYSRLLGNLCEPTQRATVADSTKLTTTANLFKRALRKHLPVLMVNYIYFNLKFHFTKQVNDELVTGIYSIFDVLSQSELTLVNASLDNPGKSFYKTLYNDYKEYGKWKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR25</accession>
    <name>G3AR25_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_155200</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376464.1">
        <property type="nucleotide sequence ID" value="XM_007376402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR25"/>
    <dbReference type="FunCoup" id="G3AR25">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR25"/>
    <dbReference type="GeneID" id="18871015"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_155200"/>
    <dbReference type="eggNOG" id="KOG0242">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001485_21_2_1"/>
    <dbReference type="InParanoid" id="G3AR25"/>
    <dbReference type="OMA" id="MQRNAFP"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061673">
        <property type="term" value="C:mitotic spindle astral microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005880">
        <property type="term" value="C:nuclear microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070463">
        <property type="term" value="F:tubulin-dependent ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045144">
        <property type="term" value="P:meiotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099606">
        <property type="term" value="P:microtubule plus-end directed mitotic chromosome migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007079">
        <property type="term" value="P:mitotic chromosome movement towards spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031115">
        <property type="term" value="P:negative regulation of microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070462">
        <property type="term" value="P:plus-end specific microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032888">
        <property type="term" value="P:regulation of mitotic spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01370">
        <property type="entry name" value="KISc_KIP3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000053">
        <property type="entry name" value="Kinesin family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF13">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF19 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="9">
        <location>
            <begin position="14"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="687"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="776"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="687"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="795"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="154"/>
            <end position="161"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="863" mass="97193" checksum="98C9364A495ADBDD" modified="2011-11-16" version="1">MQPIAPSSETKQSSITVSVRVRPFTTAESNNLIHYEESNVFLGAGSYSTANNTSNGNSNPLKRGILSAPLGIRKIVDVVDDKMLIFDPPETNPLVKMQRSAFPNKFNGRIREHRFVFDKLFDTDATQNDVYNDTTRPLLNSILDGYNATVFAYGATGCGKTHTISGTPSDPGVIFLTMKELFQRIEELKDTKIIELTLSYLEIYNETIRDLLNPQTEFKKLIIREDENSKISVSNLSCHTPTTVEEVMQLIMEGNKNRTSSPTEANATSSRSHAVLQINITQRSRTADVTAEHTFATLSIIDLAGSERAAATRNRGVRLNEGANINKSLLALGNCINALCDPRRRNHVPYRDSKLTRLLKFSLGGNCKTVMIVCISPSSQHYDETLNTLKYADRAKEIKTKLIRNQQNLDRHVGSYLKMITEQKTEIEELRNREANIIQETLAKRKHIEEETFKLLCDAIDGIRSALNNHSQDKWRKNFYLAKRKLLVAQQVDVNVMLEQAFFAQGYEFSEHTQTVISLCEQLQSKLSRQIKELETEYDKPTDIDRILNESAKQTLKRLAQNDGWGEYHTKLFQEFVKDTRAEVIKDQLTGSSVLHDLLVHGLYEFNYFSREVMKTLIDNDHDQTTDIIKGLESLLNGTFDLVVEKYVTEYVKSQLASDSHATTPASDDTDTEMETSVLFNEEVTKPARRNSILKKNPAKRSPSRRTTRDGPIIKSSPIRKPKKVVHFDISKSDSTMMDSSMNESILQDKADAEQTPITGNFLDNLNDEPDFQFDPEILSPPLPKREKLDPSLAQRGQQTTGLTKSFTNKKLTINLHDTNSSLNRHAAVIVTPTNSGTNLQPLLQEASEATPPEKSAIGVNSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR26</accession>
    <name>G3AR26_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycine zipper 2TM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_56483</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376465.1">
        <property type="nucleotide sequence ID" value="XM_007376403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR26"/>
    <dbReference type="FunCoup" id="G3AR26">
        <property type="interactions" value="230"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR26"/>
    <dbReference type="GeneID" id="18872038"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56483"/>
    <dbReference type="eggNOG" id="ENOG502S756">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099131_0_0_1"/>
    <dbReference type="InParanoid" id="G3AR26"/>
    <dbReference type="OMA" id="RWPRIRW"/>
    <dbReference type="OrthoDB" id="10624937at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008816">
        <property type="entry name" value="Gly_zipper_2TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05433">
        <property type="entry name" value="Rick_17kDa_Anti"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycine zipper 2TM" evidence="2">
        <location>
            <begin position="36"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="170" mass="17064" checksum="95E53C39AB41DA92" modified="2011-11-16" version="1">MSANDFYSSGEQGSYEPPKSNNQGNDSEGGDRGLGSTLLGGIAGGFGGNKLGGNNHGTLGTVVGAVGGAIAANKIEDAYEDHNKQDQYGRPGQHSGIGGGERRHEGGFGGERRHEGGFGGRDEGRGHGGFGGREEGHHGGFGGRDEGRHGGHGGHGGHGGHGGRGGEGRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR48</accession>
    <name>G3AR48_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nuclear distribution protein PAC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Lissencephaly-1 homolog</fullName>
            <shortName evidence="11">LIS-1</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">nudF homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PAC1</name>
        <name evidence="11" type="synonym">LIS1</name>
        <name evidence="13" type="ORF">SPAPADRAFT_67304</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Positively regulates the activity of the minus-end directed microtubule motor protein dynein. Plays a central role in positioning the mitotic spindle at the bud neck during cell division. Targets cytoplasmic dynein to microtubule plus ends, thereby promoting dynein-mediated microtubule sliding along the bud cortex and consequently the movement of the mitotic spindle to the bud neck.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Self-associates. Interacts with NDL1 and dynein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">Cytoskeleton</location>
            <location evidence="11">Spindle pole</location>
        </subcellularLocation>
        <text evidence="11">Localizes to the plus ends of microtubules and the mitotic spindle poles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the WD repeat LIS1/nudF family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376000.1">
        <property type="nucleotide sequence ID" value="XM_007375938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR48"/>
    <dbReference type="FunCoup" id="G3AR48">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR48"/>
    <dbReference type="GeneID" id="18875154"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67304"/>
    <dbReference type="eggNOG" id="KOG0295">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_15_1"/>
    <dbReference type="InParanoid" id="G3AR48"/>
    <dbReference type="OMA" id="WHVATKE"/>
    <dbReference type="OrthoDB" id="10264588at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051012">
        <property type="term" value="P:microtubule sliding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000902">
        <property type="entry name" value="Nuclear distribution protein PAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03141">
        <property type="entry name" value="lis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017252">
        <property type="entry name" value="Dynein_regulator_LIS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037190">
        <property type="entry name" value="LIS1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037647">
        <property type="entry name" value="Dynein_regulator_Lis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109925">
        <property type="entry name" value="Lissencephaly-1 protein (Lis-1, PAF-AH alpha) N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 11" id="KW-0813">Transport</keyword>
    <keyword evidence="3 12" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="148"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="195"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="377"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="478"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="439"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="491" mass="54977" checksum="6132BE00304342EA" modified="2011-11-16" version="1">MKSTILTARQQAELDKAIIQYLTPIAPPELLSQLTPLLTTNDDEIVDQYLEKKWATVLRLQKKILDLEHEVSNLRSAIDMSNANAVVLGQDRINWLPKVVKTTYLTNNVLVTSVKIHPVLGNVYCGCSDGSVYVWSLATGDMIPDKIIRGHSRNINSLAFSNEVVLGKSYVFATCSSDLSIKIWDSGSYKHIRTLTGHDHTVSAVAFSHSDSNILYSVSRDKSVKIWDLTQGNCIKTFIGHSEWVRDIDVSQVSQHGDFILTCSNDQSTRLSHASSGTGLCLIVGHTHVVETCKFLPSLSNFLLDKYITKNEDLFPSIPQELVNDPIYKVLGYKYCITGSRDNTIKVWLLPPPVIVPHRHPLPSKYNNSQAWLVAQFAGHSSWVKSLCIHPNGRFVFSGSDDKTVKIWDLSRLNNPEVGSLMGHDGFINSIDFAQLTKVEAVQNGKTEKEEEEEEEEDEEAVYDNLLKDVQSRMRCIFVTGAVDNSVRVWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR75</accession>
    <name>G3AR75_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_61826</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Endoplasmic reticulum membrane</location>
            <topology evidence="2 10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the OST1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376028.1">
        <property type="nucleotide sequence ID" value="XM_007375966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR75"/>
    <dbReference type="FunCoup" id="G3AR75">
        <property type="interactions" value="1137"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR75"/>
    <dbReference type="GeneID" id="18874070"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61826"/>
    <dbReference type="eggNOG" id="KOG2291">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031381_1_0_1"/>
    <dbReference type="InParanoid" id="G3AR75"/>
    <dbReference type="OMA" id="RYEYARE"/>
    <dbReference type="OrthoDB" id="310030at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007676">
        <property type="entry name" value="Ribophorin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21049:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21049">
        <property type="entry name" value="RIBOPHORIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04597">
        <property type="entry name" value="Ribophorin_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005131601" description="Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1" evidence="10">
        <location>
            <begin position="20"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="461"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW31250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="495" mass="56611" checksum="953FAEFFAD1EA3F5" modified="2011-11-16" version="1">MFKLNSLLILLGILFIVSASSLDAIKSSIPSSAQSLWEIPIYQRAVDLNKGFVKETDMVVLKNIDSKPQDEYYFLVNDGFDSVGNLSVFAGVLNENQRVELNYTEVVPNKVFLIKLPIPVASGSELTLKFNYVYADQLIPVPKEIAMDKIQTLLYKTNKFPLSPYTIGEYTLLFQGLTKGEEYIIHDVESTEGAPELVGPQVNAEQGLVQFGPLKGAVSPFTIVPLGLKYDHNRPLAKVTNLFRSIWIPASDVNKLSIEEYYELTNNGAHLDTGFSRIEWIKGRYESQRNHWALSHLEFPLTKDRNMDDYYYTDKVGVVSTHKLIENFLVLKPRFPLFGGWNYNFTLGWSEQLDKFLHKLHNSNDEYIIKAPLLNSLRDTSYDNVYLQFYLPENSEFLNVSSPIAYDSLEVSNELSYLDVSKGHVKVTIGYKNLIDDLYKLDVFVKYRYTSMDYYKKVAKISGLIFLALISYYLIGLVDLSIEKEKKKEVNKDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARF8</accession>
    <name>G3ARF8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_140212</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DEAH subfamily. DDX15/PRP43 sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376057.1">
        <property type="nucleotide sequence ID" value="XM_007375995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARF8"/>
    <dbReference type="FunCoup" id="G3ARF8">
        <property type="interactions" value="1535"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARF8"/>
    <dbReference type="GeneID" id="18870278"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140212"/>
    <dbReference type="eggNOG" id="KOG0925">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001832_5_11_1"/>
    <dbReference type="InParanoid" id="G3ARF8"/>
    <dbReference type="OMA" id="MKVYPLY"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022613">
        <property type="term" value="P:ribonucleoprotein complex biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17973">
        <property type="entry name" value="DEXHc_DHX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2130:FF:000001">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000007">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000324">
        <property type="entry name" value="pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000003">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044756">
        <property type="entry name" value="DHX15_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF109">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="97"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="287"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW31279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="751" mass="85356" checksum="8588E0347639BC16" modified="2011-11-16" version="1">MSERKRQRRNSLAEQVAEAAELSLRLHPRPETPNELVHHDAGEFRDLIRHKTTAAQAEALETGKVNPFTGEPFSQQYVKILEVRRDLPVHAQRDQFLETFHSSQIMVFVGETGSGKTTQIPQFVLYDEMPHLTGMQVACTQPRRVAAMSVAARVADEMDVKLGEEVGYSIRFENRTSNKTILKYMTDGMLLREAMEDHNMSRYSCIILDEAHERTLATDILMGLLKQVSVRRPDLKIIIMSATLDAEKFQKYFNNAPLLAVPGRTHPVEIYYTPEFQRDYVDAAIRTVLQIHATEETEGDILLFLTGEEEIEDACRKISLEGDELIREQNCGPLKVYPLYGSLPPHQQQRIFEPAPVNPNPKGRPGRKVIVSTNIAETSLTIDGIVYVVDPGFSKQKVYNPRVRVESLLVSPISKASAQQRAGRAGRTRPGKCFRLYTEEAFKKELIEQSYPEILRSNLASTVLELKKLGIDDLVHFDFMDPPAPETMMRALEELNYLECLSDEGDLTALGRMASQFPLDPMLAVMLIGSPAFKCSEEILTIVAMLSVPNVFVRPASARKRADEAKMHFAHQDGDHLSLVNVYEAFISPHTAEIGAHQWCRDNFLSYRSLQSAKNVRSQLKRIMEKHDLEIISVYEQVPEVEYWDNIRKALTAGFFMQVAKKKSGTKGYLTVKDNQDVLIHPSTVLSKEGEWLIYNEFVLTSKNYVRTVTTVKADWLVEIAPKYYNLDHFAKGDVRLSLERIIDRVEKSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARM0</accession>
    <name>G3ARM0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_56545</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376551.1">
        <property type="nucleotide sequence ID" value="XM_007376489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARM0"/>
    <dbReference type="FunCoup" id="G3ARM0">
        <property type="interactions" value="430"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARM0"/>
    <dbReference type="GeneID" id="18872046"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56545"/>
    <dbReference type="eggNOG" id="KOG1152">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004134_1_0_1"/>
    <dbReference type="InParanoid" id="G3ARM0"/>
    <dbReference type="OMA" id="KFANEIH"/>
    <dbReference type="OrthoDB" id="10252171at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045719">
        <property type="term" value="P:negative regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060917">
        <property type="term" value="P:regulation of (1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14004">
        <property type="entry name" value="STKc_PASK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000320">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000314">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF51">
        <property type="entry name" value="PAS DOMAIN-CONTAINING SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="926"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="246"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="803"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="851"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="820"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="853"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW31773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1185" mass="132594" checksum="C808CAA06DA9013F" modified="2011-11-16" version="1">MSENITTFSPQPSHTLPHQPQPQSQQGLSKDLFTTSQNNGKPTTPNMPSPYRKSTPSFLLYDDDEQPQPPLPTHPYTAHTAHGQVPPPPIRNTLVNKRQRPDVSTFSPPALYSPPITQNATFNSGSGSSGGGSGSGSGSGSGSGSGGSGGANTTTNILKFPIESSHAYSYAHLSPNSLALRLNVLRRSLEILKDRPDLFKSIAPQQESHSDEEIKDEEGDYNLQSNASSAALAAFFRPPIKRSGSLPLVETEERKVPQTTMRSVSSQETNDSTKPSQDHSHHNSHYSNNDLKEIVNLLENDLDFLVENSQIGSETLQDLSLEQAQTTDDDRKLKLKHDFVKSKLLYALAMPFMETSVRTTSLLESDGTQGSLKHAPIRSSTTALNMLNNNATATTPVSAGARPFHSLLAGKHSLPQSVFTIEPDMPWSVIAANDLACLMFGVSKSTIKSLTLMDLIAPQFRDFVMDKLTKYLQTSMEQSMRRRGRNKGDNGVKDILFAGEIIAISRPGDQNYAWTSLWAKKKGGIIICMFDQILCDAFDVCISSPVQHKEYRIDSVHEIAGKLVEEYEIGELHGLEQLSESLNTVLLAEEGQDEDERDEEDSDYINSTRYFTLQLNTNENIPCAITSNPLEIHPDRLEIRLKIHTMPYIAGIFVISSKSYEILSCNNAIAKNLFGKSFDEIKSKSINEIIPNFTSILEVGLDQNIDKFQLMPGLVLPEHFFRKYDAMIRCTADEDEQELFLHSNGVPGVHRDGKTVFVDIQLRASSLDTLVLWITYSRSSKNRHMLKELDKLSASTSSISLASSAPLTPSGLTRASTMNESRESKDRGVDSGANLPSQLKLLHEEQDILEFSPQRENRRGSEEIVPQSPMDVDEPAPETKVRPILKVSEEDILQQENQHLQEKMKASTHWPQEVGLVRRSKKFSEFEVIKSMGEGAYGKVVLVRHKSDPEYTIIMKCIDKERILVDTWVRDRKLGTIPSEIQIMAYLNTTPHANIMRIIDFFEDDKYYYLETPIFGNPPAIDLFDFIEIKKDISEAECQYIFKQIVSSIAHLHSKGIVHRDIKDENVIVDERGVIKLIDFGSAGYVKSGPFDVFVGTIDYASPEVLRGEKYEGRPQDIWALGILLYTMLYKENPFYNVDEIMEGDLRIPYVVSDASLALIRKILVRDIHKRPVIEDIVNDSWLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARR2</accession>
    <name>G3ARR2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Skp1 Cullin-F-box ubiquitin protein ligase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_61897</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RING-box family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376107.1">
        <property type="nucleotide sequence ID" value="XM_007376045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARR2"/>
    <dbReference type="FunCoup" id="G3ARR2">
        <property type="interactions" value="1039"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARR2"/>
    <dbReference type="GeneID" id="18874100"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61897"/>
    <dbReference type="eggNOG" id="KOG2930">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115512_2_1_1"/>
    <dbReference type="InParanoid" id="G3ARR2"/>
    <dbReference type="OMA" id="NACPLDN"/>
    <dbReference type="OrthoDB" id="983542at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16485">
        <property type="entry name" value="mRING-H2-C3H2C2D_RBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000273">
        <property type="entry name" value="E3 ubiquitin-protein ligase RBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051031">
        <property type="entry name" value="RING-box_E3_Ubiquitin_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024766">
        <property type="entry name" value="Znf_RING_H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11210">
        <property type="entry name" value="RING BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12678">
        <property type="entry name" value="zf-rbx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="51"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW31329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="117" mass="13453" checksum="697B613AB5310B45" modified="2011-11-16" version="1">MADDRMDIDEVAQESAESTPPATKSSSKQKFEVKKWTAVAFWSWDMQIENCAICRNHLMEPCIECQPNTIANGSEECIAAWGVCNHAFHLHCIKRWLKTRNACPLDNQEWTYQKFGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARU7</accession>
    <name>G3ARU7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Orotidine 5'-phosphate decarboxylase</fullName>
            <ecNumber evidence="3 10">4.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PYRF</name>
        <name evidence="12" type="ORF">SPAPADRAFT_141355</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>orotidine 5'-phosphate + H(+) = UMP + CO2</text>
            <dbReference type="Rhea" id="RHEA:11596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="4.1.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the OMP decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.23" evidence="3 10"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376142.1">
        <property type="nucleotide sequence ID" value="XM_007376080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARU7"/>
    <dbReference type="FunCoup" id="G3ARU7">
        <property type="interactions" value="1117"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARU7"/>
    <dbReference type="GeneID" id="18870368"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141355"/>
    <dbReference type="eggNOG" id="KOG1377">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030821_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARU7"/>
    <dbReference type="OMA" id="CLIKTHI"/>
    <dbReference type="OrthoDB" id="10263753at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04725">
        <property type="entry name" value="OMP_decarboxylase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000114">
        <property type="entry name" value="Decarboxylase,orotidine phosphate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014732">
        <property type="entry name" value="OMPdecase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018089">
        <property type="entry name" value="OMPdecase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01740">
        <property type="entry name" value="pyrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32119">
        <property type="entry name" value="OROTIDINE 5'-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32119:SF2">
        <property type="entry name" value="OROTIDINE 5'-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00156">
        <property type="entry name" value="OMPDECASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 10" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orotidine 5'-phosphate decarboxylase" evidence="11">
        <location>
            <begin position="32"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="8">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="8">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="8">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR614732-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR614732-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="267" mass="29436" checksum="DEB1057403BFB8CA" modified="2011-11-16" version="1">MVNNKTYTERANVHPSPVAQRLFNLMDSKKTNLCASVDVRTTAEFLALIDKLGPFVCLVKTHIDIIDDFSYEGTIKPLLELSKKHNFMIFEDRKFADIGNTVKSQYTGGVYKIAHWSDITNAHGVTGAGVVQGLKEGAKETTDEPRGLLMLAELSSKGSLAYGEYSEKTIDIAKTDKEFVIGFIAQRDMGGREEGFDWIVMSPGVGLDDKGDGLGQQYRTVDEVVSTGTDIIIVGRGLFGKGRDPQVEGKRYRDAGWNAYLKKTGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS51</accession>
    <name>G3AS51_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="19 20">Mevalonate kinase</fullName>
            <shortName evidence="20">MK</shortName>
            <ecNumber evidence="3 20">2.7.1.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">SPAPADRAFT_62907</name>
    </gene>
    <organism evidence="25">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Mevalonate kinase; part of the second module of ergosterol biosynthesis pathway that includes the middle steps of the pathway. The second module is carried out in the vacuole and involves the formation of farnesyl diphosphate, which is also an important intermediate in the biosynthesis of ubiquinone, dolichol, heme and prenylated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(R)-mevalonate + ATP = (R)-5-phosphomevalonate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17065"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.36"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:17066"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="18 20">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 20">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 20">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.36" evidence="3 20"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377043.1">
        <property type="nucleotide sequence ID" value="XM_007376981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS51"/>
    <dbReference type="FunCoup" id="G3AS51">
        <property type="interactions" value="443"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AS51"/>
    <dbReference type="GeneID" id="18874521"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62907"/>
    <dbReference type="eggNOG" id="KOG1511">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017814_0_1_1"/>
    <dbReference type="InParanoid" id="G3AS51"/>
    <dbReference type="OMA" id="LMDFNHG"/>
    <dbReference type="OrthoDB" id="1652964at2759"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004496">
        <property type="term" value="F:mevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000027">
        <property type="entry name" value="Mevalonate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.890:FF:000003">
        <property type="entry name" value="Mevalonate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019539">
        <property type="entry name" value="GalKase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006205">
        <property type="entry name" value="Mev_gal_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00549">
        <property type="entry name" value="mevalon_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290:SF2">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10509">
        <property type="entry name" value="GalKase_gal_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 20" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 20" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14 20" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 20" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="16 20" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="13 20" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="15 20" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="6 20" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Galactokinase N-terminal" evidence="23">
        <location>
            <begin position="15"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="21">
        <location>
            <begin position="137"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="22">
        <location>
            <begin position="313"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00073318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU363087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF10509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="EGW31010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="442" mass="48004" checksum="8C07960FC84A37AE" modified="2011-11-16" version="1">MTSSSPEKLASSTQSPFIVSAPGKVIIFGEHSAVYGQPAIAAALSLRCYLLVSPNDNDRITLEFPDIKLNHSWNKHDIPFEAIKSLVKLDDKGRPKVTEELVPEISDKLSPLLENIESKMHHTACFCFLYLYSNLVSQETPGMKFVVRSTLPIGAGLGSSASTSVCLASALAVLGNWVTKASFSHDEPATDKDAEELEFIDQWSLIGEKCFHGNPSGIDNAVATHGGAVMFQRNVGADNQPSVRTNIRNFPELKLLLTNTTVPRSTAKLVGGVGELTKRYPTIFKDVLNSMGNLSVEAHDIMTTSKFGELEQKKLQDLINVNHGLLVALGVSHPSLEQVKIIGDKFNIGATKLTGAGGGGCAITLVNANVDETELKKAMDEFSQHGFECFDTSLGGKGVGALYSEDISNADLFSTELFCGYRDRHEIEQALGIANNNEWRFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASA7</accession>
    <name>G3ASA7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcriptional coactivator p15 (PC4) C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_142754</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transcriptional coactivator PC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376680.1">
        <property type="nucleotide sequence ID" value="XM_007376618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASA7"/>
    <dbReference type="STRING" id="619300.G3ASA7"/>
    <dbReference type="GeneID" id="18870481"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142754"/>
    <dbReference type="eggNOG" id="KOG2712">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144950_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASA7"/>
    <dbReference type="OMA" id="WYKLLEN"/>
    <dbReference type="OrthoDB" id="2505440at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.31.10">
        <property type="entry name" value="Transcriptional Coactivator Pc4, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003173">
        <property type="entry name" value="PC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009044">
        <property type="entry name" value="ssDNA-bd_transcriptional_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045125">
        <property type="entry name" value="Sub1/Tcp4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13215">
        <property type="entry name" value="RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02229">
        <property type="entry name" value="PC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54447">
        <property type="entry name" value="ssDNA-binding transcriptional regulator domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcriptional coactivator p15 (PC4) C-terminal" evidence="8">
        <location>
            <begin position="26"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="89"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW30647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="140" mass="15817" checksum="7E59B075368DC15D" modified="2011-11-16" version="1">MAFKRSFSNRAGGNAGSSPGEETVIELDKKKQVTIRKFNNINLVDIREFYIDKDGERKPGKKGISLTEDTWYKLLENTNKIQNALDVLNGQVPKSATPSKQEKPTKKQKVENSDDEEEFEDVDISKKPPAKQEQESDADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASB0</accession>
    <name>G3ASB0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62514</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376684.1">
        <property type="nucleotide sequence ID" value="XM_007376622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASB0"/>
    <dbReference type="STRING" id="619300.G3ASB0"/>
    <dbReference type="GeneID" id="18874356"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62514"/>
    <dbReference type="eggNOG" id="KOG4821">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2928883_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASB0"/>
    <dbReference type="OrthoDB" id="188035at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016833">
        <property type="entry name" value="Put_Na-Bile_cotransptr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13593">
        <property type="entry name" value="SBF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="68" mass="7706" checksum="D073541696B37AE0" modified="2011-11-16" version="1" fragment="single">MTTLETIKQSKPYRYTSKVISFLIGQWFFVLLGVFIALAHSFPNFARQGGLIRAEYTISYGAVAIIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASB1</accession>
    <name>G3ASB1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_62515</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376685.1">
        <property type="nucleotide sequence ID" value="XM_007376623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASB1"/>
    <dbReference type="FunCoup" id="G3ASB1">
        <property type="interactions" value="432"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASB1"/>
    <dbReference type="GeneID" id="18874357"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62515"/>
    <dbReference type="eggNOG" id="KOG4821">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_987520_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASB1"/>
    <dbReference type="OMA" id="RVTICNE"/>
    <dbReference type="OrthoDB" id="166134at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051481">
        <property type="term" value="P:negative regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016833">
        <property type="entry name" value="Put_Na-Bile_cotransptr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18640:SF5">
        <property type="entry name" value="SODIUM_BILE ACID COTRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18640">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 10 MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13593">
        <property type="entry name" value="SBF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026166">
        <property type="entry name" value="UCP026166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="295" mass="33542" checksum="6A670B87C054271C" modified="2011-11-16" version="1">MTKLADGNDILTLCEVFIGNVLGAFITPALLQMYMRGTWDFGNPSHQTDGTQSIQYLYAQTMKQLGLSVFIPLFVGQVVQNIFTKQTKWAMQYFKLGKVGSFMLLLIMFQSFSTAFYQNAFNEVSHASIIFLVFFNIGIYLFFTVLTYLYARPYIVKLLLSTEPDENSTTVYKYCYKIFRPFYYNRKDTVAIMLCGPAKTAALGVSLVSSQYGAHNPKLGIILVPLVLYQAEQVMTANVLVNFMKKWIHAEDTKADDEENQLEQSEDQASQDTTAEGSSNEIRHEIIYHGDVDKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASC2</accession>
    <name>G3ASC2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Initiation-specific alpha-1,6-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62531</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376695.1">
        <property type="nucleotide sequence ID" value="XM_007376633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASC2"/>
    <dbReference type="STRING" id="619300.G3ASC2"/>
    <dbReference type="GeneID" id="18874363"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62531"/>
    <dbReference type="eggNOG" id="ENOG502QW2I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022381_5_0_1"/>
    <dbReference type="InParanoid" id="G3ASC2"/>
    <dbReference type="OMA" id="WLQSGWK"/>
    <dbReference type="OrthoDB" id="409543at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039367">
        <property type="entry name" value="Och1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834:SF1">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="31529" checksum="01EBE4D026D1C215" modified="2011-11-16" version="1" fragment="single">MWKVGLDDPEFPQHFRQYHQTWLDNSPEYTHYVMSNDERDALVLKLYADIPEITHAYQIMPQVMHRCDFSSYLLLYAFGGVYADIDTKLLKPISSWISSQPSYLDKPIDLGLIVGLESDYHKSNWANFFARRVQIDAWTFLAKKGHPMLAELINSIVEHTSWREETGQLTALLGKDEGTDVLNWGGPGRLTDLVFKYLNNVLQTDFDNYEKLIDDEFLIGIKLPIVISDAMVLPLTCMQPGRKFLKSGNLDDPLAYIHHQFAGSWRKKNKSNAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASE4</accession>
    <name>G3ASE4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_62964</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377095.1">
        <property type="nucleotide sequence ID" value="XM_007377033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASE4"/>
    <dbReference type="STRING" id="619300.G3ASE4"/>
    <dbReference type="GeneID" id="18874539"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62964"/>
    <dbReference type="eggNOG" id="ENOG502S19D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074138_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASE4"/>
    <dbReference type="OMA" id="TQEMKPR"/>
    <dbReference type="OrthoDB" id="7763451at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32343:SF10">
        <property type="entry name" value="RNA-BINDING REGION RNP-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32343">
        <property type="entry name" value="SERINE/ARGININE-RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="2"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="232"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="79"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="272" mass="29263" checksum="587E85AA3485B715" modified="2011-11-16" version="1">MSKVIASNIPSSISSDKIKEFFSFCGTVDKIEPLLNNGEYKRVEITFASEKAVDTALLLNDAEFGGAYIQVDPVHPISSPSSVAQTPSTSKSGTLEDVPQEDKPKYAIMAQLLAEGYVLSDKIIAKAIELDKQRGISSQFKDFVVDLDKRYVHFHDPESQASKNLKLAQEKAQARANDLQGIYESKVKHYLVAAANHPLGVKIHDFYKGISSDVLDVHAEAKRLADLKKATVTGSATTNPPAAASEGAPPYTVGTSPESLAAAAAATDEKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASR7</accession>
    <name>G3ASR7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Autophagy-related protein 16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_63049</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATG16 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377164.1">
        <property type="nucleotide sequence ID" value="XM_007377102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASR7"/>
    <dbReference type="FunCoup" id="G3ASR7">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASR7"/>
    <dbReference type="GeneID" id="18874568"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63049"/>
    <dbReference type="eggNOG" id="ENOG502S5CU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Autophagy-rel_prot_16_dom"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Autophagy-related protein 16" evidence="3">
        <location>
            <begin position="8"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="72"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="167" mass="19508" checksum="7435EE8F17157FBA" modified="2011-11-16" version="1">MNPAWTDDIIKQLDIRDDFEQRDIKYFTAFNQLSQQLQLQKLANKQQSPSPSLTSPTLKQEDLDRTFIIRENQQLKNENNDLISSLNMATIKNEKLEQGIKERDSTIKTLQRNNEKLKSKIDSLSLEIKEKNKTIEIINDEILMNQIQYNVLNDKVSALEAERTKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT73</accession>
    <name>G3AT73_SPAPN</name>
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        <recommendedName>
            <fullName evidence="10">Allantoate permease</fullName>
        </recommendedName>
    </protein>
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        <name evidence="8" type="ORF">SPAPADRAFT_156148</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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        <lineage>
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            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="EGW30836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="FunCoup" id="G3AT73">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AT73"/>
    <dbReference type="GeneID" id="18871044"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_156148"/>
    <dbReference type="eggNOG" id="KOG2533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_2_2_1"/>
    <dbReference type="InParanoid" id="G3AT73"/>
    <dbReference type="OMA" id="MANTIFK"/>
    <dbReference type="OrthoDB" id="1935484at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000247">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000106">
        <property type="entry name" value="MFS transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF29">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="330"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="365"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="460"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="492"/>
            <end position="510"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="550" mass="63202" checksum="EE934CA1A3FE65E4" modified="2011-11-16" version="1">MTLSINSLEKNPSSSLKKNEGSIKITSVDSHSSSESNQLVKNPFLDPKVEAYYRQVYSECNYESYSAFDPYFTWEQEEEKKLVSKLNYRVAFAACLMFVSLQIDRGNLQQAVTDNLLDDLSMNTNDYNFGNTIFKIAFLLAEVPSQLISKSLGPDIFIPIQICSWSIVAMAQAALSGKTSFFITRALIGALEGGFIADLVLWLSYFFTSKELPVRLSWFWTTLSLVGIFTSLLAFGLLRMRGILGWAGWRWLFLIEGLVTLLIGIGSFYMMVPSAVQTKNWMHPKGWFTDREVKIVVNRILRDDPSKGTMHNRQGLSLKMLFKSFYDFDIWPIFAIGLIAYIGQSTFGYYFGLINRKLGFNVFDTNLLAIPYQVLHIILLLVITWFSERVSERSLVCMIAPFFATPLIAVIRWWPGSGKEVWPTWVLNTLYLGQPYIHAICVAWVSRNSNSVRTRSISSALYNMFVQLGGILGSNIYREDDLPLYHRGNMQLFWITLALIPLLGLTKYYYYWRNMSKAKVWNSMSEDEKEHYRNYTKDEGARRLDFVFAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATB9</accession>
    <name>G3ATB9_SPAPN</name>
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        <recommendedName>
            <fullName evidence="2">Rad60/SUMO-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">SPAPADRAFT_62785</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
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            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376915.1">
        <property type="nucleotide sequence ID" value="XM_007376853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATB9"/>
    <dbReference type="STRING" id="619300.G3ATB9"/>
    <dbReference type="GeneID" id="18874464"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62785"/>
    <dbReference type="eggNOG" id="ENOG502QS09">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1295118_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATB9"/>
    <dbReference type="OrthoDB" id="3365399at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022617">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad60/SUMO-like" evidence="2">
        <location>
            <begin position="140"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="213" mass="23942" checksum="2140C9D4093F05D5" modified="2011-11-16" version="1">MATLSSDLLDRYSPETSALVWLEGKMLIPTFYTPKTLRIPPPASFNLVIDKVEDMQPTVLTCLLIENENSEQFMNIYPEFQTAAPISEEEEQPISEPTGADADADADADVVDEDDVVDLTIDDEPAIAPQVEEIDEFFVIGLKGKDNKRIEVKVSTETKIRSLLMHYLKVKGLDERTVDITHAKLIFDDEPLDMDAHVGDTELEEDFEIQIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATM7</accession>
    <name>G3ATM7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HIG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_56923</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007376961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATM7"/>
    <dbReference type="GeneID" id="18872080"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56923"/>
    <dbReference type="eggNOG" id="ENOG502RMDI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164919_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATM7"/>
    <dbReference type="OMA" id="ARMRITR"/>
    <dbReference type="OrthoDB" id="4074036at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="12470" checksum="A8C2E76359741CDF" modified="2011-11-16" version="1">MVVLNETKKQRHERFTNQLIYAGLSGMLKASLFALATGYFLSYKFNHGKNTGYFRNTYKLWWFVGCNIVGITWATDIAKINISKQAALEDEIKRNLLFEEELNQLSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATQ5</accession>
    <name>G3ATQ5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">serine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Seryl-tRNA synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Seryl-tRNA(Ser) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_63128</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.1.1.11" evidence="1"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377252.1">
        <property type="nucleotide sequence ID" value="XM_007377190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATQ5"/>
    <dbReference type="FunCoup" id="G3ATQ5">
        <property type="interactions" value="886"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATQ5"/>
    <dbReference type="GeneID" id="18874607"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63128"/>
    <dbReference type="eggNOG" id="KOG2509">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023797_4_3_1"/>
    <dbReference type="InParanoid" id="G3ATQ5"/>
    <dbReference type="OMA" id="EQNCIDR"/>
    <dbReference type="OrthoDB" id="10264585at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070158">
        <property type="term" value="P:mitochondrial seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="11">
        <location>
            <begin position="190"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="68"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="289"/>
            <end position="291"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="305"/>
            <end position="308"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="376"/>
            <end position="379"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="8">
        <location>
            <position position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW30281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="453" mass="51338" checksum="A9289B913BEE642D" modified="2011-11-16" version="1">MFRIIRKYATAKNSDTLLRPQLNIPDIIARSSLYKESVTRRQLPPTLLSDIDFIIESRPLQSQTYQQISKLKRERQQLANILKSKQDTDNCKTRLKEIKSELKPLEKYVDDLQEQIYAKAEALPNLLDETVPSDPFKEDVVQMINCSSQEDAIARRPASDLYDHKIICENFNIVNFNTASRIAGSSWYYLMNDGALLEQALVQYALSKARQRGYTMVAPPSIVKTEIVSACGFKPNDQNNEKQVYQLEDEGRSLTGTAEIPLGALHSSTVFKSGNELPKKYVGVSRSYRAEAGASGKDTRGLYRVHEFTKVELFHFTKSDLAECELEEIKDFQVEIFNELGIQAKVMNMPTSDLGSPAMKKYDIEAWMPGRGKWGELSSCSNCGEYQARRLGIRYDNQQDKLLHVNTLNGTCMAVPRVIVALIEQNYNPETNEISIPKVLQPFMDGKTKIVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATR1</accession>
    <name>G3ATR1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein FET3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">FET3</name>
        <name evidence="6" type="ORF">SPAPADRAFT_63133</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377258.1">
        <property type="nucleotide sequence ID" value="XM_007377196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATR1"/>
    <dbReference type="STRING" id="619300.G3ATR1"/>
    <dbReference type="GeneID" id="18874610"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63133"/>
    <dbReference type="eggNOG" id="KOG1263">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006504_7_3_1"/>
    <dbReference type="InParanoid" id="G3ATR1"/>
    <dbReference type="OMA" id="GMNDIED"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033573">
        <property type="term" value="C:high-affinity iron permease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010106">
        <property type="term" value="P:cellular response to iron ion starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033215">
        <property type="term" value="P:reductive iron assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13899">
        <property type="entry name" value="CuRO_3_Fet3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000024">
        <property type="entry name" value="FET5p Multicopper oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF361">
        <property type="entry name" value="IRON TRANSPORT MULTICOPPER OXIDASE FET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="5">
        <location>
            <begin position="98"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="358" mass="39800" checksum="3E2E5EB0799B3D96" modified="2011-11-16" version="1" fragment="single">SDTSKNYAFMTRVDIDMLDVIPDDLQLNGTNYIVYNEEADMPGPYDIDSIDDFFDDFYLKPLSNTTLLDDADYTITVDVIMDNLGDGVNYAFFNNISYTTPKVPTLLTALSSGEYATNSLVYGTNTNSFVLQPDDVVDIVVNNLDTGKHPFHLHGHAFQVIERHEAVDDSDPPVGFNASDHAEWPEHPMIRDTVFVQPQSYMVLRFKADNPGVWMFHCHIEWHLDQGLAILLVEDPIGIQKDPRQQITDNHKQICEKVGVPWEGNAAGNNKDFLVLTGENVQHSRLPTGFTARGIVALVFSCIAGVLGLTVIAFYGMAEVKDVEQRVARDLDVDIEDDDVEYVGEQDSSSSGVGVSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATS7</accession>
    <name>G3ATS7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Protein-S-isoprenylcysteine O-methyltransferase</fullName>
            <ecNumber evidence="3 10">2.1.1.100</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_143525</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine + S-adenosyl-L-methionine = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:21672"/>
            <dbReference type="Rhea" id="RHEA-COMP:12125"/>
            <dbReference type="Rhea" id="RHEA-COMP:12126"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90510"/>
            <dbReference type="ChEBI" id="CHEBI:90511"/>
            <dbReference type="EC" id="2.1.1.100"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.100" evidence="3 10"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377274.1">
        <property type="nucleotide sequence ID" value="XM_007377212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATS7"/>
    <dbReference type="FunCoup" id="G3ATS7">
        <property type="interactions" value="490"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATS7"/>
    <dbReference type="GeneID" id="18870552"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143525"/>
    <dbReference type="eggNOG" id="KOG2628">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065200_0_2_1"/>
    <dbReference type="InParanoid" id="G3ATS7"/>
    <dbReference type="OMA" id="IKREEAY"/>
    <dbReference type="OrthoDB" id="422086at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004671">
        <property type="term" value="F:protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007269">
        <property type="entry name" value="ICMT_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025770">
        <property type="entry name" value="PPMT_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714">
        <property type="entry name" value="PROTEIN-S ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714:SF9">
        <property type="entry name" value="PROTEIN-S-ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04140">
        <property type="entry name" value="ICMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51564">
        <property type="entry name" value="SAM_ICMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="176"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="236" mass="28193" checksum="4F1BEDBB46A24A84" modified="2011-11-16" version="1">MTTTPEQEDPIERYAQYDPDQVDLIKVMSKSLLLGMSIAFNILILYQQYTYKYYRLNIYFLFLTMFHIFEFINTVLFNITQVDDDSFILEDKEMHGITVLSIAEHFIHVNYWNISSFSSAIGLCLAIGGQIIRSVSMYTAQESFNHYIQRNHKVHNHVLVTHGVYKYIRHPSYFGFFWWFVGLQLYLNNIAVLFIGGYILWKFFKLRIEFEEGFLIKFFGQDYITYKQHTKTFMMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATZ5</accession>
    <name>G3ATZ5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DnaJ-related protein SCJ1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_63224</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377342.1">
        <property type="nucleotide sequence ID" value="XM_007377280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATZ5"/>
    <dbReference type="FunCoup" id="G3ATZ5">
        <property type="interactions" value="483"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATZ5"/>
    <dbReference type="GeneID" id="18874650"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63224"/>
    <dbReference type="eggNOG" id="KOG0712">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_0_2_1"/>
    <dbReference type="InParanoid" id="G3ATZ5"/>
    <dbReference type="OMA" id="KWHEDGD"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000002">
        <property type="entry name" value="Molecular chaperone DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044713">
        <property type="entry name" value="DNJA1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43888">
        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442618" description="DnaJ-related protein SCJ1" evidence="7">
        <location>
            <begin position="19"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="8">
        <location>
            <begin position="23"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="9">
        <location>
            <begin position="142"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="6">
        <location>
            <begin position="142"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="41615" checksum="20EA78A36F1DB5D2" modified="2011-11-16" version="1">MQLSLLSTLWLLLTLVLAKQHHDFYQVLGVDKDATDKQIRSAYKQLSLKYHPDKNPGDEQAHDKFIEIGEAYEVLSNAEKRKNYDQYGDPEGNPHGGQQFDFGDMFGQFFGGGGGRQRQRGVRRGENVQVNLNVGLSDFYNGKITEFEVRMMNDCPTCEGTGSKDKEKHTCDRCKGSGIITVQHQLGPGMVQQVRMQCDQCGGVGKVITHPCDKCKGHGVMEGPRHYDIYIKPGQPRDSNHILEGEGHRNPNWVPGDLIINIREDFSKSWGYRRVQNNLYRTEPITLKEAMNGGWERKIKFLDAEDDTLVLKRAKGQIIVDGEVEIIKGKGMPVLVEHDEVEQFGDLFIQYKVIIPGDHNRTEKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU06</accession>
    <name>G3AU06_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_157329</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377353.1">
        <property type="nucleotide sequence ID" value="XM_007377291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU06"/>
    <dbReference type="FunCoup" id="G3AU06">
        <property type="interactions" value="120"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU06"/>
    <dbReference type="GeneID" id="18871075"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157329"/>
    <dbReference type="eggNOG" id="ENOG502QWCQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034381_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU06"/>
    <dbReference type="OMA" id="WPVIVCD"/>
    <dbReference type="OrthoDB" id="9975114at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="2">
        <location>
            <begin position="16"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="413" mass="46761" checksum="0B0B7B3653ABDCB8" modified="2011-11-16" version="1">MAPPRKTSKAAPKFQPKQMVFAKMSGYPQWPAFITPVDLIPKSVMKAKKKSTDYCVMFIPDGDFYWVTDKSLELLTAQKLEKSLKDVPDEIKKQAKLLRGKKRGKSSNIKEALAAADGLDFKTFIRAFNEDAEDDEDEEEESETEENDEVGDPSAEVAQEADDDQTGNDEEVEEEVKEEEQEEVEETTQRKRAEQQEAQQEEPEPSKPTNKPQTPPESKRIKSETSDEDSSTGKAKLTEDEKQHQLWICRIKLQRSLIQRNQPTTPKDTTGLKPPTADELSVARLILYRLGDFPVSKELLKKTKIHKVLKCILRDPELEYPDSFKLHERCEELLLKWDPIVQALKLEKSSTPQKADNDGKSTTVNNGEYKKTQDESEGSGLEQTISEVEAKKENATVSSKSDLRGRTPTSVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU21</accession>
    <name>G3AU21_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative formate transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_57188</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FNT transporter (TC 1.A.16) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377368.1">
        <property type="nucleotide sequence ID" value="XM_007377306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU21"/>
    <dbReference type="FunCoup" id="G3AU21">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU21"/>
    <dbReference type="GeneID" id="18872100"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57188"/>
    <dbReference type="eggNOG" id="ENOG502QUGF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020549_1_0_1"/>
    <dbReference type="InParanoid" id="G3AU21"/>
    <dbReference type="OMA" id="VITWYLH"/>
    <dbReference type="OrthoDB" id="4829at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015513">
        <property type="term" value="F:high-affinity secondary active nitrite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140299">
        <property type="term" value="F:molecular sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006821">
        <property type="term" value="P:chloride transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015707">
        <property type="term" value="P:nitrite transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001225">
        <property type="term" value="P:regulation of chloride transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000292">
        <property type="entry name" value="For/NO2_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00790">
        <property type="entry name" value="fnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30520">
        <property type="entry name" value="FORMATE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30520:SF6">
        <property type="entry name" value="FORMATE_NITRATE FAMILY TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01226">
        <property type="entry name" value="Form_Nir_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="411"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="439" mass="48838" checksum="6F474582E7E5DB93" modified="2011-11-16" version="1">MDDSQYLTTHEAALAVVATAMKKARLRIDTLIINAIMGGILFTTGGMLHVTTQAYLPNIYQENPGLISILAGLSYPIGLFYVVIMGVDLFNSNILFFSTAICRGAVSVFDLLISWIVSYFCNLIGNIFVCYVICHYSAIAQTDMWVQASHEIAINKDSFSFIENFLKGVAGNFYVCLAIYLQLMAKPLHVKFLMMALPVFTFVTIGFTHAIADMTVLITALINHAPVSVGEVAWKIFLPGALGNIVGGSFFGIVITWYLHIYVVEQDQERLKLPKYEVRDEQPELNIDSRVVRQRRTSSYVEEVDDVMAEKQNQDSYEPVPYFSNSSGSGLRLRATNTRGTTFSVASKKHRSPKNVFPVYGMGPPLAREKTIAGGTPGLPEPEDTDIEDEDSVNQYTADYIGDKLKRVITNKPRRKSKAQDIEAGYKSSPSIFRLEGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU64</accession>
    <name>G3AU64_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_73170</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the USE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377411.1">
        <property type="nucleotide sequence ID" value="XM_007377349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU64"/>
    <dbReference type="FunCoup" id="G3AU64">
        <property type="interactions" value="148"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU64"/>
    <dbReference type="GeneID" id="18875471"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_73170"/>
    <dbReference type="eggNOG" id="ENOG502SZ4C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093900_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU64"/>
    <dbReference type="OMA" id="YHESIQD"/>
    <dbReference type="OrthoDB" id="4008582at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019150">
        <property type="entry name" value="Vesicle_transport_protein_Use1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13050">
        <property type="entry name" value="USE1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13050:SF7">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN USE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09753">
        <property type="entry name" value="Use1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="253" mass="28933" checksum="70DAA4699ED63A48" modified="2011-11-16" version="1">MTSIHTIETIIDQYSTEINSLNIPTLTHFNHDTIITISPYISQFKLEQIKHSLSNINQELHKFKSNKSYAQVYDKFTHLFVDVLDEKIYVNSKLIDIYEQQNRPVEKVEQESTVEQGPAPEEDLPSLRKRLLAGGKTSSLLDSNQDITKVNEYHESIQNDILNELSGLTSNLKSSALKFSSKILGEDSNILSETQENISKNANLFQATNRNLNNYLENKTGGRIGLWFLIKITIALVMIFLFMIIFIKIIPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUK5</accession>
    <name>G3AUK5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_63398</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377532.1">
        <property type="nucleotide sequence ID" value="XM_007377470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUK5"/>
    <dbReference type="FunCoup" id="G3AUK5">
        <property type="interactions" value="163"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUK5"/>
    <dbReference type="GeneID" id="18874730"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63398"/>
    <dbReference type="eggNOG" id="KOG4155">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065016_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUK5"/>
    <dbReference type="OMA" id="CVCLDRS"/>
    <dbReference type="OrthoDB" id="10251741at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055087">
        <property type="term" value="C:Ski complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070478">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065004">
        <property type="term" value="P:protein-DNA complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051510">
        <property type="entry name" value="SKI8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44090:SF1">
        <property type="entry name" value="SUPERKILLER COMPLEX PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44090">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="279"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="381" mass="41780" checksum="4F91509E74631379" modified="2011-11-16" version="1">MGKQYIFTVNASQAHTSDILGVAITNKYTISVSSDGYAKFWDNKKDEIHNPSEYVKSCFINKIGIHHIAAYENTLPSSTVKVTLLAFACFDGSVVFKYYVNDDFDTLKDWEDTKMSNCWVPQFYRDPESKQDYFIVTKTTGSTSVNHLKITEQDGEVAIAIEQTGELDPATSSFPNSLAVCPTKNKKAAIGYINGDVLLYDFENLKLVYTFHSPDLQQSYKSSTTSIPRVLAFSPGGTLLAVARDNQAAGSITLYDVDHGENVGTLTTPSHNTKSTIGGFAHQGWIMDLSFDEEGKLLASGGFDKCVRVWNLETGEREATINISASDLDDVEQGEHDTSVVSGVRFIKKGVRGGAGGDSNEGLCVVSFDRGIRWYREAGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUP3</accession>
    <name>G3AUP3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LicD/FKTN/FKRP nucleotidyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_63439</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377570.1">
        <property type="nucleotide sequence ID" value="XM_007377508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUP3"/>
    <dbReference type="STRING" id="619300.G3AUP3"/>
    <dbReference type="GeneID" id="18874749"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63439"/>
    <dbReference type="eggNOG" id="ENOG502QREF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_689089_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUP3"/>
    <dbReference type="OMA" id="TDANEPR"/>
    <dbReference type="OrthoDB" id="444255at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009644">
        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF28">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE TRANSFERASE FKTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="5">
        <location>
            <begin position="127"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="435" mass="51767" checksum="98A78919A8468D84" modified="2011-11-16" version="1">MTNQPRSRQLKLSKDMFNHPPSIDSQLQEIGRKGLSRQLTDHEQSYYNSLVECKQYTDANEPRYFRMAVIRMDDPRNRDQEWGWHYDWRFFNGALNYDRDGWTVQELGHRTNIILDRLLRNWNRFAQQKGIISWIMHGPLLSWYWDGLMFPFDVDIDIQMPMVDLIRLAREFNNTLIVEDPQEGYGKYLIDVNPYMHNRGISEGSNHIDARFIDVDSGIYIDITALSKSNAQPPPDYDEDKLVDLHHKKGDKYNDRRKHFYTFEELSPLRYSMLQGVPVFIPSVITPRLMFEYQEGLNWFEFNGWYFVTKLNLWVHENTVAAIYDSRDIADENGGIDKSKLLDKVKNMSDKEVYQLLESNDDLLVEYYLTKSLTDLHEQESKFLFDETGRDISRKMSESTFQQYSELTKQFKLNKPLRKALYDYESIERVKYHKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUR4</accession>
    <name>G3AUR4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mif2/CENP-C cupin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_143424</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CENP-C/MIF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377591.1">
        <property type="nucleotide sequence ID" value="XM_007377529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUR4"/>
    <dbReference type="FunCoup" id="G3AUR4">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUR4"/>
    <dbReference type="GeneID" id="18870546"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143424"/>
    <dbReference type="eggNOG" id="ENOG502S47H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_527842_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUR4"/>
    <dbReference type="OMA" id="AKMFFVQ"/>
    <dbReference type="OrthoDB" id="1939643at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019237">
        <property type="term" value="F:centromeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051455">
        <property type="term" value="P:spindle attachment to meiosis I kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028386">
        <property type="entry name" value="CENP-C/Mif2/cnp3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025974">
        <property type="entry name" value="Mif2/CENP-C_cupin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028929">
        <property type="entry name" value="Mif2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16684">
        <property type="entry name" value="CENTROMERE PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16684:SF11">
        <property type="entry name" value="CENTROMERE PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11699">
        <property type="entry name" value="CENP-C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15624">
        <property type="entry name" value="Mif2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mif2 N-terminal" evidence="7">
        <location>
            <begin position="4"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Mif2/CENP-C cupin" evidence="6">
        <location>
            <begin position="399"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="92"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="324"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="55"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="102"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="210"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="239"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="332"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF15624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="501" mass="56265" checksum="FE68F766B2F17B25" modified="2011-11-16" version="1">MDLLDLGTRGRKTQLELPSNINKDENGMEDLNDFFNDSDVGVTSNRGISRVREPPSTSTSRKLYQETSLGSRKEFTDVARKIDFTNISPAIQMIGPTKTGDKSTTATGTTGGNVKNKKSPLRSPLHEHQGAALFTRSDSDSDRFEQDPAQFDQDMDSFEQDPTQFDVEEEEEQQQEIEVRKRPRQSKGASRLTKNMALGKSSKRKMQVESESDDAEDSYILDDTTQSDRLLSPPPTLKKPARQASKPRTKPVQKPLITKPSPLPSPPPDGLRRSKRTRIAPLAFWRNERIIYTRAGNVDGDTTLVQDIRKIPLQEIKEVVHMPEVAPVEQRKKPKPKSKSPKTRSPKEYSVDADVPGAEWFDDKYLTMSVNDENGNPKEENIAYTIDGGEVQDTDENIKVTKLFDVDRDFCSTGVIEIPPEGLKSTKLTGSSLFIFHVISGLNQVTINKTTFLANSGCSFQIPYNNSYSITNIGETDSKLLFMQVRKPMVIEEELDDSWQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUT0</accession>
    <name>G3AUT0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_143419</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377607.1">
        <property type="nucleotide sequence ID" value="XM_007377545.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUT0"/>
    <dbReference type="GeneID" id="18870545"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143419"/>
    <dbReference type="HOGENOM" id="CLU_2967535_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUT0"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="59" mass="6762" checksum="F08073D467D16C5F" modified="2011-11-16" version="1" fragment="single">TPEHPTRNIREYPRTSYPQHKRVPPVPHPVWGCPSMFPMLLACGYRLYLPMPLACGSTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUT9</accession>
    <name>G3AUT9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_63650</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Tom70 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377796.1">
        <property type="nucleotide sequence ID" value="XM_007377734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUT9"/>
    <dbReference type="FunCoup" id="G3AUT9">
        <property type="interactions" value="798"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUT9"/>
    <dbReference type="GeneID" id="18874851"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63650"/>
    <dbReference type="eggNOG" id="KOG0547">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017516_1_0_1"/>
    <dbReference type="InParanoid" id="G3AUT9"/>
    <dbReference type="OMA" id="CRNGMAY"/>
    <dbReference type="OrthoDB" id="2942533at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000374">
        <property type="entry name" value="Mitochondrial proteins import receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="150"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="184"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="400" mass="44769" checksum="944C11663B2F4823" modified="2011-11-16" version="1">MFDLTALTIYGGFSNKSIEQVLERVLRKHAVKIVSGKEKKLALPSAATIGSFFNAFVEERNPEGITEESEGGDQFLYKALQQIATNTQEGYEEADSLINQAVGAYEQSEPENKGPYAVAMEYLAAFQFLKNDPSNATISIEKAINLKPRPRSYIFRALINADKSSYQEALEDFKKAEEMDPECPDIFYHLGQLFYLTGDLTQAETNFNKAKELHPENVYAYIQLACIAYKNGSGESANEKFTEARLKFPTSPEVPNYYGEILADKGDIQGAVRQFEIAARLQEKLERFSVGALPLINHATVISRESLERIDEAQELLVRACELDPRSELARISLAQIKLQKDEVDDAIVLFEESSDLARSFEEKVQATSFAEATKMQKRIKSDPILTAKIAEVMRQSGAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUV6</accession>
    <name>G3AUV6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Metal resistance protein YCF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_144773</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Cooperates for the ATP-dependent vacuolar transport of bilirubin and glutathione conjugates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377813.1">
        <property type="nucleotide sequence ID" value="XM_007377751.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AUV6">
        <property type="interactions" value="306"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUV6"/>
    <dbReference type="GeneID" id="18870662"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144773"/>
    <dbReference type="eggNOG" id="KOG0054">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_27_3_1"/>
    <dbReference type="InParanoid" id="G3AUV6"/>
    <dbReference type="OMA" id="CFETGMR"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015431">
        <property type="term" value="F:ABC-type glutathione S-conjugate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18595">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18603">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000020">
        <property type="entry name" value="ABC metal ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000450">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000001">
        <property type="entry name" value="ATP-binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056227">
        <property type="entry name" value="TMD0_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF443">
        <property type="entry name" value="MULTIDRUG-RESISTANCE LIKE PROTEIN 1, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24357">
        <property type="entry name" value="TMD0_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="152"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="440"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="519"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="555"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="951"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="994"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1082"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1184"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="17">
        <location>
            <begin position="293"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="16">
        <location>
            <begin position="618"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="17">
        <location>
            <begin position="955"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="16">
        <location>
            <begin position="1274"/>
            <end position="1520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="848"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="856"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="869"/>
            <end position="878"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW30047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1531" mass="171339" checksum="E2DBDDD9C6F4BA56" modified="2011-11-16" version="1">MLLSVGGSASSGSILSEGNNSTTLKNSGWLSCSDPNGDGWGPLSPTYHDIAPCFLQGVLFGSSAILMILVGLQQLIYLRNKKIQVGNRKTWNFYVKLLLVALQLGFQLALVATSTQSGIIHLALILNAVATVVAWGIHYLEQYKSTIPNGVVLFYWLFQIILNLGRTANIQLRTGTGDVFSIFVILSTVNAFFILVIETAFPIQPLNNYTRTLKVSPYDRANVFSRITFDWMGGLMKKGYVQYLTERDLPALPNKLKATTTSNRFQHYWDSQAVEKPSLFLAIAKAFGGQFMLGGLFKGLQDSLAFIQPQLLRLLIKFVNDYSESVKRGDPIPLTRGFLIALSMFIVSVVQTACLHQYFERAFELGMKIKSALSSSIYNKSLVLSNESKQESSTGDIVNLMSVDVQRLQDLVQNLQIIWSGPFQIVLCLASLHGLIGNSMWAGVAIMVIMIPLNAVIARIQKRLQKTQMKYKDERSRLINEILNNIKSLKLYGWETPYLDKLRHVRNDKELKNLKKMGIFSAFTNFTWNLAPFLVSCSTFAVYVLTQDKSLSTDLVFPALALFNLLSFPLAVVPMVITNVVEAQVAISRLTKFLTEPELQVDAVVKAPKAKKLGDTAVSIKNGTFLWSRAKGDQNYKVALSNINLDAKKGELDCIVGRVGSGKSSIIQAILGDLYKLDGEVTLHGKIAYVSQVPWIMNGSVRDNILFGHKYDPEFYDLVIKACALTVDLSILPKGDKTEVGEKGISLSGGQKARLSLARAVYARADVYLLDDPLSAVDEHVGKHLTDHVLGPHGLLKTKCRILATNNIKVLSIADSLALVSDGRIVERGTYDDIMKQESSKIRQLIDSFGKKRDGSSTPSSESTNSQADAKKNELEETKVDEEEIDLQELDSDCDFECGSLRRASEVSLDQESEIDDEIEDEDAKARKEHLEQGKVKWDVYKEYAKACNPVNVMIFLSFTVISFVINVASNFWLKHWSEVNSQYGYNPNVVKYLGVYFLLGIGFSGASLIQNCFLWIFCSIQGSKKLHNRMAVSVLRAPMTFFETTPIGRILNRFSNDVYKIDEVLGRVFSMFFSNTVKVTLTLIVICYSTWQFVLLILPLGILYIYYQQYYLRTSRELRRLDSVSRSPIFANFQESLTGVSIIRAYGKEERFKHLNQTRIDRNMGAYHPAINANRWLAVRLEFLGSVIILGAAGLSILTLKSGHLTAGLVGLSVSYALQITQSLNWIVRMTVEVETNIVSVERILEYSRLTSEAPEIIPDNRPPANWPVDGEIHFKDYSTKYRPELDLVLKNINLDIKPREKIGIVGRTGAGKSSITLALFRIIERFGGSITIDGIDTSTIGLYDLRHKLSIIPQDSQVFEGSIRSNLDPTDEFTDDQIWRALELSHLKDHVTKMYEERDTDIEIEGPLHVRVTEGGSNLSTGQRQLMCLGRVLLKLNNSNVLVLDEATAAVDVETDQILQETIRTEFKDKTIITIAHRLNTIMDSDRILVLDKGEVAEFEAPEVLLKKKESLFYSLCEQGGFINDEDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUY1</accession>
    <name>G3AUY1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_63687</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377838.1">
        <property type="nucleotide sequence ID" value="XM_007377776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUY1"/>
    <dbReference type="FunCoup" id="G3AUY1">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUY1"/>
    <dbReference type="GeneID" id="18874869"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63687"/>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000134_20_0_1"/>
    <dbReference type="InParanoid" id="G3AUY1"/>
    <dbReference type="OMA" id="EAENVGW"/>
    <dbReference type="OrthoDB" id="10057496at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24188">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24188:SF29">
        <property type="entry name" value="GH09064P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="170"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="198" mass="22200" checksum="40484D39C81B436C" modified="2011-11-16" version="1">MSIPELTQEEMDVVIYDARVGDLETLEEIFAEIPPLTLLNIKDDITLSTPLHMACANGHFDTVKYLLSILPKEDAVNWIKAKNESGNTALHWAGYNGHLEIVKLLIDEYEGDVFIKNDAGHDVLYEVESNGQEEVENWLLKKFAIEETVNEDGDTKITYTAGTESKLADEQARDASFKSATPEVPEDLDKKTDKLSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV85</accession>
    <name>G3AV85_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Carboxypeptidase</fullName>
            <ecNumber evidence="13">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_63508</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in degradation of small peptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Release of a C-terminal amino acid with broad specificity.</text>
            <dbReference type="EC" id="3.4.16.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="13"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377654.1">
        <property type="nucleotide sequence ID" value="XM_007377592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV85"/>
    <dbReference type="SMR" id="G3AV85"/>
    <dbReference type="FunCoup" id="G3AV85">
        <property type="interactions" value="897"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV85"/>
    <dbReference type="MEROPS" id="S10.001"/>
    <dbReference type="GeneID" id="18874787"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63508"/>
    <dbReference type="eggNOG" id="KOG1282">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008523_10_4_1"/>
    <dbReference type="InParanoid" id="G3AV85"/>
    <dbReference type="OMA" id="GDWMKPF"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046938">
        <property type="term" value="P:phytochelatin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.410:FF:000001">
        <property type="entry name" value="Carboxypeptidase Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008442">
        <property type="entry name" value="Propeptide_carboxypepY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF113">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE CTSA-4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05388">
        <property type="entry name" value="Carbpep_Y_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 13" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Propeptide carboxypeptidase Y" evidence="14">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF05388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW29888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="525" mass="58586" checksum="46080AF65E9F88BD" modified="2011-11-16" version="1">MKFSTVATAATLAIASVNGLSIPNPLSSLQQTLKLENAFEAADIKQFIGDMMKQSLPMETKKLWSEMMLKFPDTMSKLSFKGPPAKKLRNLSSQFIHVLDDAFPGHGLRIKSTPESLGIDTVKQYSGYLDVKEEDKHFFYYFFESRNDPANDPIILWLNGGPGCSSMTGLFFELGPASINAELKPVHNPHSWNNNASVIFLDQPINVGYSYSSGSVSNTIAAGKDVYAFLELFFKHFPQYSKSQFHIAGESYAGHYIPVFATEILSHPERSFNLTSVLIGNGLTDPLVQYEYYEPMACGEGGEPSVLSPEECQAMNNSIPRCTSLIQSCYDSESVWSCVPATIYCNNAQMGPYQRTGRNVYDIRTMCEGSNLCYSQLEYVDQFLNQPEVMEALGAEVEKFESCNTNINMNFMFAGDWMKPYHTRVAELLDADLPVLIYAGDKDFICNWLGNQAWTNRLEYKNAKGFSKAEVKKWKVGGKAAGEVKNFDKFTFLRVYGGGHMVPFDQPESSLAMVNSWVAGDYALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVA2</accession>
    <name>G3AVA2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mitochondrial presequence protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_57410</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377671.1">
        <property type="nucleotide sequence ID" value="XM_007377609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVA2"/>
    <dbReference type="FunCoup" id="G3AVA2">
        <property type="interactions" value="182"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVA2"/>
    <dbReference type="GeneID" id="18872118"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57410"/>
    <dbReference type="eggNOG" id="KOG0961">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006065_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVA2"/>
    <dbReference type="OMA" id="CVEGPFW"/>
    <dbReference type="OrthoDB" id="4953at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000015">
        <property type="entry name" value="Putative zinc metalloprotease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000031">
        <property type="entry name" value="Putative zinc metalloprotease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43016:SF16">
        <property type="entry name" value="METALLOPROTEASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G07610)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43016">
        <property type="entry name" value="PRESEQUENCE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="2">
        <location>
            <begin position="54"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="3">
        <location>
            <begin position="196"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1024"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1033"/>
            <end position="1060"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW29905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1060" mass="121667" checksum="A1B84B317853DE35" modified="2011-11-16" version="1">MTQNQTQFVKQTSFEVEYAPSHITKWRSSRTGLQMTYINQPSPIVNGYFAVATEIENSSGCPHTLEHLVFMGSKKYPYKGLLDNLGNRLYSSTNAWTSVDQTVYTLTTAGWEGFKTLLPIYLDHLFNPTLTEEACLTEVYHIDGKGKEKGVVFSEMQGIESQSWFVSFQKMQETLYAPNSGYSSETGGLMKELRHLTNNQIKEFHKSMYRPDNLCVIITGSVEENELLEVMTEFDNELPELPTTPNKRPFVDSHPHDEPLQEVLIKEVEFPEIDESMGELLISWIGPNGNDTLVNSAIDMIGAYFTDSPISLFNKNLVEIEHPLATDIDYTTDDYFRTAINFTVNGVPTAKLAEVDTKIKDLILSQTKPENIDMKYLKQVINQQKLKFVSDTEKSPSLFSNIAILEFIYGNPDGTDLSKWTKDLQEYETMLNWTAEQWSELVKTYFVDNKSATILGKPSAKLNDLIKKQNKQLNKEIKEKYGKEGLQKLQEKLEAAQAKNDIPIPEELLTKFSKPDPSKVEFIKTKSYNAGYTKSIVDNNTNQYIQDDKFSELLTNDTPADFPLFIHLEDFKSQFTTIKLIMSSTRIDPQLLSYMSIMEELFSLSIQLPDEYIPYEKVISEINNDLIEFQLDNGYENQFLELIGVNIKFESTKYQKAIDWLLKVTRYTIFEESRVKVIIEKIINSLPDKKRNGELMMYSSQYRHMFEQTSLRKAQDAIFTELFYKDLLEQIDQGNFAKIQQDLNTLRQQLFKLDNIKVFILGNTSNLKSPVSAWTKFTQECLEQDKQHDDLFTPEPFAELPRSYQFRSDIGDACNKEAYFVNIPAAESTHLMSLTKIPTNYLEDDIFKIALASEFLTAVEGPFWRGIRGTGLAYGANIRRDIETGYLSFSIYRGSDAKQAWLTAKEIVEKYASKSIEIDDISIENCIAVIVNECANSQENNYEAANFKITDNVFKRRGPNYYELFLKKLNKLTADDIVYALNKYFVNLFKPETSVVFTSLPPDKAPELAEHFHEVGFNVNFEEIASDDHGEVESEEEEEEEHTDEESDCTDSESEEDDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVL5</accession>
    <name>G3AVL5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ribonuclease III</fullName>
            <ecNumber evidence="3">3.1.26.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_57457</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ribonuclease III family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.3" evidence="3"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377730.1">
        <property type="nucleotide sequence ID" value="XM_007377668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVL5"/>
    <dbReference type="FunCoup" id="G3AVL5">
        <property type="interactions" value="278"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVL5"/>
    <dbReference type="GeneID" id="18872122"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57457"/>
    <dbReference type="eggNOG" id="KOG1817">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026251_1_0_1"/>
    <dbReference type="InParanoid" id="G3AVL5"/>
    <dbReference type="OMA" id="TINGHNE"/>
    <dbReference type="OrthoDB" id="2392202at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034963">
        <property type="term" value="P:box C/D sno(s)RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030847">
        <property type="term" value="P:termination of RNA polymerase II transcription, exosome-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19876">
        <property type="entry name" value="DSRM_RNT1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000001">
        <property type="entry name" value="Ribonuclease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00104">
        <property type="entry name" value="RNase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040540">
        <property type="entry name" value="RNase_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011907">
        <property type="entry name" value="RNase_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044449">
        <property type="entry name" value="Rnt1/Pac1_DSRM_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207:SF0">
        <property type="entry name" value="RIBONUCLEASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="RIBONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18497">
        <property type="entry name" value="RNase_3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="10">
        <location>
            <begin position="132"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="9">
        <location>
            <begin position="276"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW29964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="488" mass="55100" checksum="B3791CF1A441C1CF" modified="2011-11-16" version="1">MDVAKLEYAAKQLQKNLKLITKQAPDIYQLKLVVDSLDSGAAIEFQHNELITMAARIKSRYKIGKLPIFDEIITGKIGMTDTDMESLKQLQEENLVCEVPPIINTAQADGVDSSDLPPLPPIRDPVLEARVFTHRSYMNNRSSTSEETTINGHNERLEFLGDSILNTLVTIIIYNMFTTASEGQLSKIRSDLVNNKTLGHFSLAYGMDKRLKTSIVDLAVTPDQKLFADVFEAYIGALAVERDLDLEDIRQWLDQLYTPMLNNLRDTYIKPPIDREAKTQLYSMIGTNESHPQYRTIQQGNPVTGVDFVVNCTMEGELLGSGSGNSIKEASLRAAMDALSHEEKLEKYYLHRQEIELPIRKRRNELKELKREEKLKQIQQELAEAAKLPPLPPPSPIRTSCFPLEPDYSLPLDHEAKNELYALIGKKARVQPTYIVSKTSDNKHIVTLKIRGVTIVSAEDSSKKKAMSRAARTLLNHKEAMYEISKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVV3</accession>
    <name>G3AVV3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_144677</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377764.1">
        <property type="nucleotide sequence ID" value="XM_007377702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVV3"/>
    <dbReference type="FunCoup" id="G3AVV3">
        <property type="interactions" value="188"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVV3"/>
    <dbReference type="GeneID" id="18870654"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144677"/>
    <dbReference type="eggNOG" id="KOG0714">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043579_1_0_1"/>
    <dbReference type="InParanoid" id="G3AVV3"/>
    <dbReference type="OMA" id="ARSREHN"/>
    <dbReference type="OrthoDB" id="1507364at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051100">
        <property type="entry name" value="DnaJ_subfamily_B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015399">
        <property type="entry name" value="DUF1977_DnaJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908:SF3">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09320">
        <property type="entry name" value="DUF1977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="J" evidence="6">
        <location>
            <begin position="21"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="80"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="91"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW29998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="324" mass="36242" checksum="92B8D1BC01906A3A" modified="2011-11-16" version="1">MSFTKDQEAIVLKVLSYKPHQFYEILSVEKSANESEIKKSYRKLAIKCHPDKNPHPRSAEAFKLLNKAWGVLSDPGKKKIYDQTGSDPDSRFASAASSSGASTGSFGNGGPYYRGGGVPFDDDIFNLFFGGGSGASPFGGGGGTTFQFGNGFTFQSFGGADPFVRHRHQFRGHNQQNTQHQDRRQEASLSESLKALAPILLILIVPIFSALFSDSNSAPEYSFHKTRDYSVQRSTPRFNIPFYVNKKFTEKYSGKSPQQLKNFDYKIENVYIQDKRAKCSREQIRKNEMIEDAQGWFFTDTEKLAAAENMAMPNCEILRGLSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADZ2</accession>
    <name>G3ADZ2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SCF complex F-box protein MET30</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_63949</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372128.1">
        <property type="nucleotide sequence ID" value="XM_007372066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ADZ2"/>
    <dbReference type="FunCoup" id="G3ADZ2">
        <property type="interactions" value="199"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ADZ2"/>
    <dbReference type="GeneID" id="18874952"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63949"/>
    <dbReference type="eggNOG" id="KOG0274">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_103_1_1"/>
    <dbReference type="InParanoid" id="G3ADZ2"/>
    <dbReference type="OMA" id="GIAHVWS"/>
    <dbReference type="OrthoDB" id="5580488at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043224">
        <property type="term" value="C:nuclear SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140299">
        <property type="term" value="F:molecular sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046685">
        <property type="term" value="P:response to arsenic-containing substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046686">
        <property type="term" value="P:response to cadmium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="F-box_SpPof1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="E3 ubiquitin ligase complex SCF subunit sconB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F-box protein MET30"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051075">
        <property type="entry name" value="SCF_subunit_WD-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19872">
        <property type="entry name" value="UBIQUITIN LIGASE SPECIFICITY FACTOR/HREP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19872:SF9">
        <property type="entry name" value="UBIQUITIN-BINDING SDF UBIQUITIN LIGASE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="6">
        <location>
            <begin position="156"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="295"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="335"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="375"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="414"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="521"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="561"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="483"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="483"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="615" mass="69820" checksum="F894A5DE8EA7542E" modified="2011-11-16" version="1">MDTLEASQITPVLENKSIIMEKENNSPTSTKTVDIQVEQTTQVEQPQEENSAITPFLITHLSNQNLIPRDNFHKYCYRHNPDVTCNKLTDDIKMKDIQNRLERLPNRDQEAISHVWSIFNAAPVPQQKLILQGLLSQCCFPQLSFLSQEISSLIRIDFISALPQEISLKILCYLDCRSLCNAAQVSRKWKSLADDDRVWHHMCEQHIDRKCPNCGWGLPLMHMKRAREMQEDDDIRPIKRRHSEASAVKTAITAAKKEPAPTPPVIRKRPWKAVYSERYKLEKNWRKGIYTVKSFTGHTDGVTCLQFNRKYLMTGSYDTTIKIWKIETGECLKTLTGHTKGVRSLVFDNQKLITGGLDSTIKVWNYHTGECIATYRGHDDAVVSVDFSNKSIVSGSADHTVRIWHVDSRTCYTLRGHTDWVNCVKIHSASNTLFSASDDTTIRMWDMNTNQCLRVFGGASSNGHIAQVQCVIPFVYRQALVEDDPGSESEGETDLQHPPLLDSVQNDQPHEPLPANYPTHLLTSSLDNTIKLWDVQTGKCIRTQFGHIEGVWSINSDTFRIISGAHDRLIKVWDLQNGKCLHTFGNDSSVSCVGLSDSRFAAGLESGHVKMYFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AED5</accession>
    <name>G3AED5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_48691</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373135.1">
        <property type="nucleotide sequence ID" value="XM_007373073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AED5"/>
    <dbReference type="FunCoup" id="G3AED5">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AED5"/>
    <dbReference type="GeneID" id="18871256"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48691"/>
    <dbReference type="eggNOG" id="ENOG502SEET">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_328171_0_0_1"/>
    <dbReference type="InParanoid" id="G3AED5"/>
    <dbReference type="OMA" id="FNDSYLD"/>
    <dbReference type="OrthoDB" id="4085524at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="218"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="464"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="714"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="287"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="17"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="490"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="516"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="536"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="574"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="629"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="765"/>
            <end position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="858" mass="94199" checksum="DFB16CF4739D0A57" modified="2011-11-16" version="1">MFRRKTRSTHTTAYTGVNQPAPANNQPNSSAVAAALTIGNSLKTTAVKPITQKPTPKSTQSATAPPTKTYGGSLFKRGSRASIGSMQSSQSQQPQQPPHTPHPFRRFSSGSTVTNSSAYSPTKVHDIDDSFTDSYIDNITDESTRVYLKNQQNLKDLKLEHKPAKGAAVPKKTSSTTTTANVTPKMVKKYIPTPNGIKIVEVPQETYDKEVARCNSMRGSGFSRSSSLRNVSKHVKPIPRSSSLSNVTTARKVAPKRSVSAITPPTNLESMTEVVDLEDKLGKSDEMIEQQRKLDLLQKQIDEEKKLAHELEIKKQEYESLKAKRVQEEQSLNKAIEKEISMDNVEESTTIIQHAEEEETNGEVLEHPEQETNGDVLDHSSVVENTEHALIIEKPELSAVDTLSEHDEETQAVHQETPVPPPVVISESADSNNFPSLDPGVNELGIISQYGNFRSNELLAIASQDSKTKEEEAPPTIEMPAPENYLQDEQAPHEPNNDNHVEPRDLIAPPHHDNASSKSSVYSNESPVRKPIKSAMKNSTSSLPSQGLAQVLAQPQTSAPPPAAKPKSIVKPNPAQQAYLSLTTAENTRMNSKLSHSDLNGASAYPQFHNQKSQVPPQKRLSHQSLRNSQPQPTQLSQQSLRKSPPPPAHQIRTLRPNSMPMEPQGMASRQFRHTPVSNAGHTIVQPIPPHPTTAPGYTSPSKARAQELYAKAQARPKSVFKPTAQEATKTQNKEKAISNRNTRMTLRDPMAPSATQSHGSKPLDSSVDTTPTSDSGNRRRKFKSRFVDSDDEDGPVRHAGNFFSSRFNDSDDEHLQPPVTVLQDVRDVEVAPAAAAAAKEKKKFGKLRKLFGTKRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEF6</accession>
    <name>G3AEF6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_58947</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373156.1">
        <property type="nucleotide sequence ID" value="XM_007373094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEF6"/>
    <dbReference type="STRING" id="619300.G3AEF6"/>
    <dbReference type="GeneID" id="18872691"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58947"/>
    <dbReference type="eggNOG" id="KOG1601">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_782038_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEF6"/>
    <dbReference type="OrthoDB" id="515401at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000007">
        <property type="entry name" value="Nitrogen regulatory AreA, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF281">
        <property type="entry name" value="BOX A-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="8">
        <location>
            <begin position="269"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="235"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="319"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="245"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="261"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="329"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="340"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="355" mass="38917" checksum="792B976B94A69AA1" modified="2011-11-16" version="1" fragment="single">MLSSSNTTPIKTQQSQSQRTGGGGLSQLSKQLNQFNRFAHPSQSLETMLPSQHRQSITQHHEPVPTLPSNSFSFALDPYALEGPQDMDLNSHSATSSYDAKPLFDDFIHTPATLSSSASTIIYDNSHNNNNHNSFFQNPSKRSSTSTATPSSLLREESMMSLPGMMNRPFTPLNMSYKQDSQFMRHSPSITSLSSGPPPITTSASFNTTTQKFVLPSQSIESSYFENPHEVDYFSPNSLPANLDKKKKLKTKTTKSKRNSPDSTGWPGSSGGVSCSNCHTRTTPLWRRDPEGQPLCNACGLFLKLHGVVRPLSLKTDVIKKRQRGGGNTASTSTTPSSTKKTEKIDKEKEAAAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEI8</accession>
    <name>G3AEI8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Spindle pole body component Bbp1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_47834</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372162.1">
        <property type="nucleotide sequence ID" value="XM_007372100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEI8"/>
    <dbReference type="FunCoup" id="G3AEI8">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="GeneID" id="18871151"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_47834"/>
    <dbReference type="eggNOG" id="ENOG502RQ8I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044220_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEI8"/>
    <dbReference type="OMA" id="NQPTIDY"/>
    <dbReference type="OrthoDB" id="4025341at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="101"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="318"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="41403" checksum="E138397825320CA1" modified="2011-11-16" version="1">MIKYLFGVGEDDSRPLVDEPTIDYNETIHSSTPSSQGNLRQTRKSILKDTTGSSHNNPDIPVIGDLSDSELDYDDDLIHDINLVREVTQTLSSGYDGLGNHATRISIPDDYPGKYPRSRERTSNLSEQKNDPNWDIRASKGGRTSNYSKSYESTEQVYESTIDRKLDQLRIEVDNELKNYKNKPSTGNDDTTSQLRELNTRLKARTQYLSKLSQLTKAYNEEQNESTIVPPDIMEKYDNMKTEYLEELHRTQTLYLAYYRLLEKHLQFRKTKSTKNDVTVSKSATKSVIPMREKIKLIQSKTSDSTIKNLCGNVLSDVNKWEQDRDSLLKEKEQLQKDLRQMKSEKEALQLKLNNKESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEQ8</accession>
    <name>G3AEQ8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_64772</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373096.1">
        <property type="nucleotide sequence ID" value="XM_007373034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEQ8"/>
    <dbReference type="GeneID" id="18874998"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64772"/>
    <dbReference type="eggNOG" id="ENOG502RUYB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_316909_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEQ8"/>
    <dbReference type="OMA" id="KETNVHK"/>
    <dbReference type="OrthoDB" id="2575524at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442446" evidence="2">
        <location>
            <begin position="22"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="358"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="746"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="437"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="485"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="586"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="633"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="691"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="747"/>
            <end position="758"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="920" mass="96451" checksum="5BDA1D6D2B4833BD" modified="2011-11-16" version="1">MTYLLLLLLAHLFGLVDLGAAMFVGANTEISNDVQSSASELEETSVSVASELQVSVETQDSFDLEDPDDFVHSIEIDPHDPVYRIKLDDGSRCYNARQDTFGNCYIEAYSGPIDIKEDPIIKIAGYNSNFTLSNTYNNIEVPVAGEESVDTDVAAEQPIELESPDDIIRFPSASITVKNGIWEYEPDISGPMVSLRIDGHLSVIEITHHTLEDIDYNSDSFSVTESEEQTVESNETTDVPEDTIMPVEPTTTTEENGGGAIYIGNPRTLLHDVTVDPIVDIPVGPYTLNIPIFTLAQGRFDPFHSEGPTDSSDSVEPEETTEIGSGSNTIEFPLYTGIVVGPDGPLIINPLEPVETLATDNTDQPQDTIVPEGPTNPEQTGVTVDPSQARDPINPVQTETPPSPEDPPKPYVEPEETSEPQDPKVPANPETPADVGQTETPVNAQTSEGPVAPIGTSKPADPVNQSEDPVIPDAPANTESQVNPKDTETSVKPEDPVDPEITATVDTPVDPEHTETPVVPEDPANPEDPANPVGSANPEDPVGSANPEDPANPEDPANPVGSANPEDPANPEDPVNPQDPAAPQGPVNTQVLTATSVDPEQTENPSDPTKEELVEDDPSDPTPVTSNVAPADQTTTQTQGSQSGNPSNPQSPSNPTSNYSQATSASQYSNPSSNQGNSNSAVSQGNGGNQGNSSPSVQVSSNPTTPTSNTSPSNPANNNNNAAAAAAAATAATAATAATATVNNASQPTNNVASPQGTNQGGSAGQSGYASTPLQPNQLQTIVTDVTMVETISDSFTTYVTTITEEVTGVVEADSTTVAIITPVVEPITVTYTSVEGGSEVVYTTTITPIAEPYTVTFTGSDEAGSNSQPSVVAADPEENIQSDSNQPESTLVSFDSASTRVSTHYLALVLGLVLTVCFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AES7</accession>
    <name>G3AES7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sedlin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58960</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373169.1">
        <property type="nucleotide sequence ID" value="XM_007373107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AES7"/>
    <dbReference type="FunCoup" id="G3AES7">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AES7"/>
    <dbReference type="GeneID" id="18872696"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58960"/>
    <dbReference type="eggNOG" id="ENOG502SG8J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097630_0_0_1"/>
    <dbReference type="InParanoid" id="G3AES7"/>
    <dbReference type="OMA" id="KCYLRTI"/>
    <dbReference type="OrthoDB" id="18320at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006722">
        <property type="entry name" value="Sedlin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12403">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04628">
        <property type="entry name" value="Sedlin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="22171" checksum="85B2E0EAC381B950" modified="2011-11-16" version="1">MTAPAATSNTPPLKQTTSETSVSQASQVATKTPFNPIQFVSIISRTDKPLYIQSFGIDDTSTDTTENANKFLKYNFLSHMALDIFTSPESLSLRGGQSQTQQQTDANVVLLFIQDQVMVYGYETNNGLKIIVGLDQSFVIQDQRILRQLFLDIHKCYLRTIFNPFSKVNDEEDDDDSVLSTPTFDKNIKKIVDGWTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEZ3</accession>
    <name>G3AEZ3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF202 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_145281</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372208.1">
        <property type="nucleotide sequence ID" value="XM_007372146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEZ3"/>
    <dbReference type="GeneID" id="18870681"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_145281"/>
    <dbReference type="eggNOG" id="ENOG502S764">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107661_2_1_1"/>
    <dbReference type="InParanoid" id="G3AEZ3"/>
    <dbReference type="OMA" id="YRRYQGN"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38646:SF1">
        <property type="entry name" value="DUF202 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38646">
        <property type="entry name" value="YALI0F00814P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="14177" checksum="0D30711320E41BAC" modified="2011-11-16" version="1">MSQPLAERRFSSLDILSNYLKLDIRAYQRTYEGAYTRTAIGCLSFSILIIKMFSKEFLPVGTVYTMYGTLLYFLGVYQTNRVDRFYVPSKQMEMFKTAGDTVLMLTVFSLCSYIAVLALILRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF66</accession>
    <name>G3AF66_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="7">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_145406</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Lipid-anchor</topology>
            <topology evidence="7">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="7"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372266.1">
        <property type="nucleotide sequence ID" value="XM_007372204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF66"/>
    <dbReference type="FunCoup" id="G3AF66">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF66"/>
    <dbReference type="GeneID" id="18870686"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_145406"/>
    <dbReference type="eggNOG" id="ENOG502QRZZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021855_2_1_1"/>
    <dbReference type="InParanoid" id="G3AF66"/>
    <dbReference type="OMA" id="ICLRDIP"/>
    <dbReference type="OrthoDB" id="421038at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903561">
        <property type="term" value="C:extracellular vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030445">
        <property type="term" value="C:yeast-form cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000038">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF10">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 7" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005131598" description="1,3-beta-glucanosyltransferase" evidence="7">
        <location>
            <begin position="22"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="9">
        <location>
            <begin position="390"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="493"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="506"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="548" mass="61520" checksum="0E5686D40179C679" modified="2011-11-16" version="1">MQLLPFIFLIGLLNVLKIGESFTATDVPSIETIKVAGNKFFLSESGNQFFIKGISYQKTRQEGELYDNSKEPHYIDPLANPITCLRDLEYLKELGVNVVRVYQILPTANHDVCMNAFADAGIYVLADLSEPYLSIRRDFPHWDTELFARYKEVIDAMHQYDNILGFFAGNEVTNSYINTDASPYVRAAIRDSKRYIDEQNYRQIPVGYASNDDASIREHLANYFVCDFDGSGDGRADFFAINVYEWCGYSTFSTSGYRDLTSSFSDYPVPAFFSEYGCNTVSPRPFTEVDALYGVSMSKIWSGGIVYEYFEEVNHYGIVIEKKDGQIVKLPDFDTLKSKFNSVSPVGINIKEATPSPRVTCSRPDELWNVAHTLPQTPDTGKCECLRESLSCIIPENRSFEEEQVLKSLCEDVDCEPVNGNGKAGKFGQFSDCDSRTRASYVLNKFYEQTGRKEETCSFQGKGILVNPIGFVDLDTKFSSDGRNCLSLLHSNSEEPEHNFDKSPPTKVTPQSNENSTASNMDSSAPQNEGVAGILFLSFMLAISPLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF87</accession>
    <name>G3AF87_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_57969</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372288.1">
        <property type="nucleotide sequence ID" value="XM_007372226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF87"/>
    <dbReference type="GeneID" id="18872275"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57969"/>
    <dbReference type="HOGENOM" id="CLU_2943288_0_0_1"/>
    <dbReference type="InParanoid" id="G3AF87"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="60" mass="6564" checksum="4E86DB099551173E" modified="2011-11-16" version="1">MKDENVSLNSKDCKYQIMNLNMSLLAIPQPPATLPPSEISPLLSSQLGYTLTDIAAHVCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFA4</accession>
    <name>G3AFA4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_53282</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372305.1">
        <property type="nucleotide sequence ID" value="XM_007372243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFA4"/>
    <dbReference type="STRING" id="619300.G3AFA4"/>
    <dbReference type="GeneID" id="18871773"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53282"/>
    <dbReference type="eggNOG" id="ENOG502S0WX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025455_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFA4"/>
    <dbReference type="OMA" id="ANQNGMH"/>
    <dbReference type="OrthoDB" id="3364175at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF23">
        <property type="entry name" value="THIAMINE REPRESSIBLE GENES REGULATORY PROTEIN THI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="2">
        <location>
            <begin position="158"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="517" mass="59726" checksum="FD08D79B20AE40A8" modified="2011-11-16" version="1">MSKLTIEFVRANLLTLVKDAVAILPEDDKIRKLVRIYLNICETNYFYVHHTNFYKKVEEFIVKRREQDLEFFIRDWPNLVILLAMMAISAGFEYMDDNQPIPNFVLDTSPGLIYYYAALPFAGFLIDQKSIESVQGLLIFGVFMTTNKLENFQMFDGGYLFMNLALEIAIANKLYLKEPYRNYSIEDQEVYKRLWWSCYTMERRFGINLGKSEIISPDDITVELPSNVPALNNSLGCSNYLSQSSMIDLNYIFRDIAELYQPRTKSKDNEARISIDPKMVRKLVEDAEECKINFPVYAQVDRLNPTSQLYRGSIHLNLAYYLAKIYIGKPFLLYKVDNFRRLAEQSDYESAFVDHLSSICIDAAFCTIELLSELDKHNKLGLFSCTDINFCNISLFTILVFLKIDRSETTLLFLRKGLKILKTMSNGCSSAKSSLQKLKKLDNLVSDFAELDEMQKDMNTLFSVETAAKSFPTHEDNNDPILQFDGVKDVDISQLFEGIDDFLGQVDDDIYQQLQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFD9</accession>
    <name>G3AFD9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_131119</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372340.1">
        <property type="nucleotide sequence ID" value="XM_007372278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFD9"/>
    <dbReference type="FunCoup" id="G3AFD9">
        <property type="interactions" value="732"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFD9"/>
    <dbReference type="GeneID" id="18869553"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131119"/>
    <dbReference type="eggNOG" id="KOG0201">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_23_1"/>
    <dbReference type="InParanoid" id="G3AFD9"/>
    <dbReference type="OMA" id="NMFIVME"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000499">
        <property type="entry name" value="Serine/threonine-protein kinase KIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF10">
        <property type="entry name" value="FI20177P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="7"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW34928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="440" mass="49985" checksum="A0BDB5F2BFC3B806" modified="2011-11-16" version="1">MGAANKYYFEKCIGKGNFGDVYRAKSISSGKTVAVKVVCLDDSSDEMNQVIQEIHFLSKLRNKFITQYIESYLHGYNMFIVMEYCGGGSCSDLLRCHKHLDEGVVSYIIKGVLSGLSYLHAEHKVHRDIKSANILLTEDAQVKLADFGVSGEITMTRLKKNTFVGTPFWMAPEVIARSKTKGESGYNEKADIWSTGITTIELVTGYPPLSQYDPLKVLFEIPKKRPPILQGCSYSANIKDFVKYCLLKDPSKRPSSNTLLHHHFITRGSNVREKLIELINAKNVRMESKVKKPRHKISPANEHNSALSTPIVWTFETNRNLHSFLNIDLAMEQDESQGPTKHICQVEEKEEDKFKEETEMSHELVPTAPRSPVGRFAKDKACMLFYCLERVYHRGKNEFTKSAVAELMKSIQQHEQEQPGLCHTIVEEFERLLTTANNKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFU8</accession>
    <name>G3AFU8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CUE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_131594</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372500.1">
        <property type="nucleotide sequence ID" value="XM_007372438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFU8"/>
    <dbReference type="FunCoup" id="G3AFU8">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFU8"/>
    <dbReference type="GeneID" id="18869592"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131594"/>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037447_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFU8"/>
    <dbReference type="OMA" id="RRSADHN"/>
    <dbReference type="OrthoDB" id="9942608at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14372">
        <property type="entry name" value="CUE_Cue5p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000064">
        <property type="entry name" value="Similar to CUE domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041807">
        <property type="entry name" value="Cue5/Don1_CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16461">
        <property type="entry name" value="TOLL-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16461:SF5">
        <property type="entry name" value="TOLL-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00546">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="CUE" evidence="3">
        <location>
            <begin position="73"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="120"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="225"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="128"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="147"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="350" mass="38588" checksum="FC3977C67CDA459D" modified="2011-11-16" version="1">MSKKNEKLNVISSEPVESIDETPAKKESPVPAKKESPIPKTDVAKEKHDSTEEVPINESEEQAPVQPPRPVDPIEQITKDLKDAFPNIEDKIITACLIASQGNPDPAFNALLYISDPSFKPEIPAPQTSSSTTKSVDTTKTLTDDELLARKLQKEFELEDDRRRRAARRKGSHPQPKQSRRHSEDFDDSPDEFEQIKETFSQGLEEARTTLNGWVSGIAKKIDGVTNSAPPKVPSKENPKLFGALGGSSFENRRRTTKFDEDPEILTNDFHDKIQLSNNDEGDEHAPPKPARPSTEGKIPQPENEAGKKWQPLESEVPASSDAFLVTDSEDEGDDAVAAAETAPVPPSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG87</accession>
    <name>G3AG87_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_53536</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372638.1">
        <property type="nucleotide sequence ID" value="XM_007372576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG87"/>
    <dbReference type="STRING" id="619300.G3AG87"/>
    <dbReference type="GeneID" id="18871792"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53536"/>
    <dbReference type="eggNOG" id="KOG4194">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025148_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG87"/>
    <dbReference type="OMA" id="GHETEEY"/>
    <dbReference type="OrthoDB" id="4019115at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF22">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN COMPLEX ACID LABILE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="669" mass="77437" checksum="50EE4D3EB43F94BD" modified="2011-11-16" version="1">MDRNEPQSNYNEDISSLIELSTFPEELLRLIFSFLPANVVYEKILGAGDWDQLQLSHVQLVAFRFIFQKSLLIRSRNSERVGFFRIVYPNNEDEKSMGCHGFQEGYQLMRKLINFNFKLDSPVFPKDITLLFRFRDRMKEAPPHEVDDLDAEIEYFIMILELLTFDNFYKDRIKSVNFHLIYDTAPTPSLMLNQSKIMQKIEMLSRKSTKIVIYNRNKVKWAIGLNPFQLDRLQELYLWNNGFNDFNIVNYFHGMSPHLKILHLTGNDITSLRGLIIPSTLEQFIITANNITSLEGPNFQDAPNLRIFDASINAVTEITNKVKFSNNLKIFRIPYNRIQSFRNFEIPNKLQTLSLSKNLLTGVDYIRLPATLIELFLNGNYFKSLPDSFFYTCENLTKLNLSDNLIDDLDDLGNLPSNLEELILDNNEIDNNDLTNILCLNKLKTLSMISNGLMSINGNQFPKTLQVLKLDKNELSELINIDFGDNLCKLSLSGNNLNKFNIEQYKLKFPPTLRFIDLSYNPLEDVGSLFIPDSARVLRLNGINIPFNNHFLSHLPKGIQELELNHTGNIQHYPCLDFATHLPNLKSISMERNHIVSMEQMKFPFTLERLSLEGNLLSKIPFGLIPSSSKYLQFNNNKISIERLKLPKLPQLEYFGVCGDQTNLIRQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGC1</accession>
    <name>G3AGC1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acireductone dioxygenase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Acireductone dioxygenase (Fe(2+)-requiring)</fullName>
            <shortName evidence="11">ARD'</shortName>
            <shortName evidence="11">Fe-ARD</shortName>
            <ecNumber evidence="11">1.13.11.54</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Acireductone dioxygenase (Ni(2+)-requiring)</fullName>
            <shortName evidence="11">ARD</shortName>
            <shortName evidence="11">Ni-ARD</shortName>
            <ecNumber evidence="11">1.13.11.53</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ADI1</name>
        <name evidence="12" type="ORF">SPAPADRAFT_58471</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 3-(methylsulfanyl)propanoate + CO + formate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14161"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:49016"/>
            <dbReference type="ChEBI" id="CHEBI:49252"/>
            <dbReference type="EC" id="1.13.11.53"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 4-methylsulfanyl-2-oxobutanoate + formate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:24504"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:16723"/>
            <dbReference type="ChEBI" id="CHEBI:49252"/>
            <dbReference type="EC" id="1.13.11.54"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
        <text evidence="11">Binds either 1 Fe or Ni cation per monomer. Iron-binding promotes an acireductone dioxygenase reaction producing 2-keto-4-methylthiobutyrate, while nickel-binding promotes an acireductone dioxygenase reaction producing 3-(methylsulfanyl)propanoate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the acireductone dioxygenase (ARD) family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.54" evidence="11"/>
    <dbReference type="EC" id="1.13.11.53" evidence="11"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372671.1">
        <property type="nucleotide sequence ID" value="XM_007372609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGC1"/>
    <dbReference type="FunCoup" id="G3AGC1">
        <property type="interactions" value="284"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGC1"/>
    <dbReference type="GeneID" id="18872464"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58471"/>
    <dbReference type="eggNOG" id="KOG2107">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090154_1_1_1"/>
    <dbReference type="InParanoid" id="G3AGC1"/>
    <dbReference type="OMA" id="WYMDESQ"/>
    <dbReference type="OrthoDB" id="1867259at2759"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00878"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010308">
        <property type="term" value="F:acireductone dioxygenase (Ni2+-requiring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010309">
        <property type="term" value="F:acireductone dioxygenase [iron(II)-requiring] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02232">
        <property type="entry name" value="cupin_ARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000099">
        <property type="entry name" value="1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03154">
        <property type="entry name" value="Salvage_MtnD_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004313">
        <property type="entry name" value="ARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027496">
        <property type="entry name" value="ARD_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23418">
        <property type="entry name" value="ACIREDUCTONE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23418:SF0">
        <property type="entry name" value="ACIREDUCTONE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03079">
        <property type="entry name" value="ARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8 11" id="KW-0408">Iron</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="3 11" id="KW-0533">Nickel</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <note>for iron-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <note>for nickel-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <note>for iron-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <note>for nickel-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <note>for iron-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <note>for nickel-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <note>for iron-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <note>for nickel-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW35259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="177" mass="20765" checksum="13BBFC28D7D9D49D" modified="2011-11-16" version="1">MVEVYYHDNKDTPEDFTKAHNSGEPVSLEKLAEIGVFYRNVKTLDELNQIAKDRDYKNRDVVNLDLDAFNNDLDAYNAKMAQFYKEHYHEDEEIRYVTAGEGYFDVRDKQDRWIRAKLSPHDLLILPAGIYHRFTLTDELKQVTAVRLFKDEPKWEAINRDTGKVSNARSEYVQSIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGJ1</accession>
    <name>G3AGJ1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP-dependent RNA helicase FAL1</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">ATP-dependent RNA helicase fal1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_53612</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372742.1">
        <property type="nucleotide sequence ID" value="XM_007372680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGJ1"/>
    <dbReference type="FunCoup" id="G3AGJ1">
        <property type="interactions" value="684"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGJ1"/>
    <dbReference type="GeneID" id="18871801"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53612"/>
    <dbReference type="eggNOG" id="KOG0328">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_0_1"/>
    <dbReference type="InParanoid" id="G3AGJ1"/>
    <dbReference type="OMA" id="DTIHGDK"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097078">
        <property type="term" value="C:FAL1-SGD1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030874">
        <property type="term" value="C:nucleolar chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000849">
        <property type="entry name" value="ATP-dependent RNA helicase DBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000031">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13 17" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="26"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="57"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="239"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="26"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW35330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="400" mass="45420" checksum="9000D2CB074C8CD4" modified="2011-11-16" version="1">MDEFDRELDNELEFSHKSTKGIKVHRTFESMKLKPDLLKGIYGYGFEAPSAIQSRAIMQIISGKDTIAQAQSGTGKTATFSIGMLQVIDTKSHDCQALILSPTRELAVQIENVVKHLGDYMNIHTHALIGGINVGQDVKKLQTSQPQIVSGTPGRVLDVLKRRNLSPRHIKILILDEADELFTKGFKEQIYEIYKQLPPGAQVVVVSATLSPEVLEMTNKFTTDPVKILVKRDEISLSGIKQYYVQCEKEEWKFDTLCDLYDNLTITQAVIFCNTKLKVNWLVEQMRKQNFTVVGMHGDMKQDERDSIMNDFRTGNSRVLISTDVWARGIDVQQVSLVINYDLPTDKENYIHRIGRSGRFGRKGTAINLITKADVQTQKELEKFYSTKIKEMPMNINDIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGQ8</accession>
    <name>G3AGQ8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_146579</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372802.1">
        <property type="nucleotide sequence ID" value="XM_007372740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGQ8"/>
    <dbReference type="STRING" id="619300.G3AGQ8"/>
    <dbReference type="GeneID" id="18870722"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_146579"/>
    <dbReference type="eggNOG" id="ENOG502QVYJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007695_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGQ8"/>
    <dbReference type="OMA" id="IHRKDAN"/>
    <dbReference type="OrthoDB" id="3364175at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051127">
        <property type="entry name" value="Fungal_SecMet_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF6">
        <property type="entry name" value="PROLINE UTILIZATION TRANS-ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="515"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="562"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="592"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="16"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="54"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="126"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="54"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="70"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="771" mass="87970" checksum="A2C1229320956806" modified="2011-11-16" version="1">MTQFVTIEPRKKVTKACDYCKKRKYKCSGVAPCDLCTKKKIPCQFSIVDRRTTKGNQARDKNDRQRRESSSSTVTAVSVMSTNTPAQRQNSKPDIPLDDQLIQHNKLIATKMSGYIPKSLQPLLSFPLEKDKDGSSLSSTNDEDDEEEEEEDEEDEGLSNDSGKSTRLLFDTAGNLRYIGESSPLSLLFESRNIFTSTIGESEFTGDPQGVNIIDGTARINVVPPMQLPRRDHCNVMIKFFENNINQTWYIFDMKDFKLNIVDYIYENPIRAPVEKLSLLHLVFSLGLLFAEKANSYLVEEMKPAGVTSTDFFESGFNLMRNIIDDGKLWLSQAYFLVYFYYQSTSKRSTSFLMLSTAIRNAQTLGLHRKFINESFRDQNYVTHRRKLWKSLYICDRISSILLGRPLLISDYDWDDTGDDDDTVYAPDGMTLDIQYTSLNYNARIAIILGKIIQNFYIDGIVDLLRAERIAIDLKTWSINLPSAIQIDKILDVEGSTATTSPVDNPLHKVKSDNYGLLLIHMSQLYGIILLSKPFFMYLLFKRNKNESPNKRSKQEAIMSNFCAASIKSSVLMIQLIHFFKEHNPHRMESYVLVNCCFMASLILGLTILYRQTNVYEPDEFTIDILAEKLMVAKSILLFYSEVSATAARFHTIVTKMYRALENAFKKSHPFPQALPQHLTFDSPESVTSQPQFPIKTPAVIQSQLQSPSSFPHTFEIATSTELEHLMNFQQFFVPSVSNSVITTNTEKRNELDAFMYNIGINDLLFEGAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGQ9</accession>
    <name>G3AGQ9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Hydroxyquinol 1,2 dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_53661</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the intradiol ring-cleavage dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372804.1">
        <property type="nucleotide sequence ID" value="XM_007372742.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGQ9"/>
    <dbReference type="STRING" id="619300.G3AGQ9"/>
    <dbReference type="GeneID" id="18871804"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53661"/>
    <dbReference type="eggNOG" id="ENOG502QU2V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046727_1_1_1"/>
    <dbReference type="InParanoid" id="G3AGQ9"/>
    <dbReference type="OMA" id="AMGPKTT"/>
    <dbReference type="OrthoDB" id="5238185at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018576">
        <property type="term" value="F:catechol 1,2-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009712">
        <property type="term" value="P:catechol-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03461">
        <property type="entry name" value="1_2-HQD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.130.10">
        <property type="entry name" value="Aromatic compound dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039390">
        <property type="entry name" value="1_2-HQD/HQD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007535">
        <property type="entry name" value="Catechol_dOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000627">
        <property type="entry name" value="Intradiol_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015889">
        <property type="entry name" value="Intradiol_dOase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050770">
        <property type="entry name" value="Intradiol_RC_Dioxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33711">
        <property type="entry name" value="DIOXYGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G02910)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33711:SF7">
        <property type="entry name" value="INTRADIOL RING-CLEAVAGE DIOXYGENASES DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00775">
        <property type="entry name" value="Dioxygenase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04444">
        <property type="entry name" value="Dioxygenase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49482">
        <property type="entry name" value="Aromatic compound dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00083">
        <property type="entry name" value="INTRADIOL_DIOXYGENAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Intradiol ring-cleavage dioxygenases" evidence="7">
        <location>
            <begin position="127"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="319" mass="35942" checksum="26EFF58CCF4A6A11" modified="2011-11-16" version="1">MSQEFTDYVKSSFGPKATPKARRILTSLIQHIHDFTRENHITVDEWMFGVDFINRIGQMSDARRNEGILVSDVVGLEALVDTLTHASDSVGHTSSAIIGPFYRDNSPEYPYGGSIIQKEVGGEKTWVSGIVTDTNGKPLAGAKIEVWHCAPNGLYEQQDPDQPDYNLRGTFYADEQGKYAFIGLRPTAYPIPYDGPAGDLLQMMDRHPMRPGHIHWRVSHPEFHTLITQIYDRDCKYTKDDSVFAVKDDIIVDFKKVASDDKELGDKGVQNRLDYDIVLTLEEEIQKTRASVVAKHKSIEEELEAHHKAIADKIAKLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH28</accession>
    <name>G3AH28_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_48449</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372870.1">
        <property type="nucleotide sequence ID" value="XM_007372808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH28"/>
    <dbReference type="STRING" id="619300.G3AH28"/>
    <dbReference type="GeneID" id="18871221"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48449"/>
    <dbReference type="eggNOG" id="ENOG502S6B4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1027338_0_0_1"/>
    <dbReference type="InParanoid" id="G3AH28"/>
    <dbReference type="OMA" id="RKITHYA"/>
    <dbReference type="OrthoDB" id="3981267at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="209"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="271" mass="30629" checksum="2A5E2D9872574E4B" modified="2011-11-16" version="1">MVAPYQNSAISIFEFFPTIAVKQFCNFVKVFFITIYVNIMEASSELPRITIGNINDSSFLDDKSPHPTPVIPPRYHPLRSTSLVHDKTINEFYSKRQISSGPYLYPPQHNTPLGLTSPTFGLGTAAADPYLCVNQAELYQAYPAPFQPPFYNVKTPNVPTSGGASDNINSLNPRKAPNMNFREKITRWLETISDTNEVIVNDTSFSSSNSDVDIDIEDLEDRLQAQAEKLTRFATRQYRYDSEPVALFEGEYVNEEEGSDYADDSYQVLNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHU1</accession>
    <name>G3AHU1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_134665</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373839.1">
        <property type="nucleotide sequence ID" value="XM_007373777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHU1"/>
    <dbReference type="FunCoup" id="G3AHU1">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHU1"/>
    <dbReference type="GeneID" id="18869842"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134665"/>
    <dbReference type="eggNOG" id="KOG2904">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018398_0_2_1"/>
    <dbReference type="InParanoid" id="G3AHU1"/>
    <dbReference type="OMA" id="MPRIPYS"/>
    <dbReference type="OrthoDB" id="269872at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102559">
        <property type="term" value="F:peptide chain release factor N(5)-glutamine methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006451">
        <property type="term" value="P:translational readthrough"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004556">
        <property type="entry name" value="HemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050320">
        <property type="entry name" value="N5-glutamine_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00536">
        <property type="entry name" value="hemK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18895">
        <property type="entry name" value="HEMK METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18895:SF74">
        <property type="entry name" value="MTRF1L RELEASE FACTOR GLUTAMINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="4">
        <location>
            <begin position="106"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="305" mass="34640" checksum="2DFD2995CA77052B" modified="2011-11-16" version="1">MARLSPRLIRQAKSISSLLPPLLRANRTIEAAQRELNWIKRELPANEWVKAVEARDRLVPLQYILGNVPFGNITVKCKPGVLIPRSETEAWVIDLSEKVKEVANDEIGIVDACTGTGCIPLLLAHELDKVGVKTRVDAFDVSVEAVNLANENKDSVEGSSGVNFQIGDVFNVNILEHIGVEKVDLVTSNPPYIPFHDYKKSMFEHGIERSVKLYEPQLALVGEYEFYLELLQNIVIPGHAKAFVFEIGYEEQAEFVYEYLRDYSAWRVGIWCDYNDNVRCVIGWNVDTEFVKLKDMCDHLYEPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHV4</accession>
    <name>G3AHV4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CAP-Gly domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_65423</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373851.1">
        <property type="nucleotide sequence ID" value="XM_007373789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHV4"/>
    <dbReference type="STRING" id="619300.G3AHV4"/>
    <dbReference type="GeneID" id="18875042"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65423"/>
    <dbReference type="eggNOG" id="KOG4568">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_324819_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHV4"/>
    <dbReference type="OMA" id="LWHERDH"/>
    <dbReference type="OrthoDB" id="2130750at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CAP-Gly" evidence="2">
        <location>
            <begin position="23"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="135"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="193"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="275"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="619"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="815" mass="94916" checksum="E0427559994D0C45" modified="2011-11-16" version="1">MTEYSVGSKVIVKTTPGIIRYIGTTQFAPGTWYGVELSQPNGKNNGSVEGVQYFHCKPNHGVFVRQSMLEPQKEMSPDLNNVNRIIDKLQNKLKLVTSESSGYKERISELMKESERTMERIGGLESKLEMQLLENEFLKSSNLELTTQLEELSDRYKKLDTEFHLIKEELEINQELEHEFSFVDVEQYSSDDIKSIVERNKQLESALVALKKLSEETENNLKKEIDELKLSQGIEERLEQFEELEKITEHLTHENHELSKTIISLNKTIGELNEIHDLDKQLEETQKQSEAELRKQVSNLTKEIEVNKEKIRTLEDKNKSLQKNIDKSVPEKDLTDYELELKSTQQTLRDLKAKSRADSIKLELVENRYSIVTQHTIPHELSDVILKFKLTISDLNVLSTKFPKKLSGQIYAYFVVMELQEFFKFVHMLLEYNPDLDLNLELLDPILNAITAFIQGDDLQQGIIDEFTQVGKAIHTIIEFSELEGLQLVDNLWVRFINSLLRHQHEFSKSIVEQYPNLRKQLDQLVQEDTAIDITKEIEFPKGKPNFRILFKQINDCVNELHSLITKDELSETQLSLSLENIPHIDFAYGHSIDPVSIYDIKPEPEQIAEKSEVPDSLVSELNSKILQKDQEISDLKLNITMLEQNMKSLTSQNTLNLENLQAKLIKQEQQIQSYLETIKSLELEKEDLKTQLTLVEKNTQEYDLVFNDLKSQHQYNENISFMEQISQLKQINKMRMATPKQDYSWLKKTSRTRWNETTPLMELSRDLRSIALDVKAVHIATTSTWRPKKNLPKYANLLIEERYTHYKSRRNNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHV5</accession>
    <name>G3AHV5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cell division control protein 31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_59689</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373853.1">
        <property type="nucleotide sequence ID" value="XM_007373791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHV5"/>
    <dbReference type="FunCoup" id="G3AHV5">
        <property type="interactions" value="876"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHV5"/>
    <dbReference type="GeneID" id="18873030"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59689"/>
    <dbReference type="eggNOG" id="KOG0028">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061288_18_1_1"/>
    <dbReference type="InParanoid" id="G3AHV5"/>
    <dbReference type="OMA" id="EFFMIMK"/>
    <dbReference type="OrthoDB" id="343296at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005825">
        <property type="term" value="C:half bridge of spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030474">
        <property type="term" value="P:spindle pole body duplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Centrin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF48">
        <property type="entry name" value="CENTRIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="43"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="116"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="152"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00070017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="183" mass="21343" checksum="6881A8E424509A9C" modified="2011-11-16" version="1">MAFSGRKSTLDYQPQTTSPFPGNKRTPGQQQQQPTVKQELLDEQRIEIREAFSIFDMNGDGYLDYHECKVAFRALGFDLPKRQVLDIITEYDTDDTNLINYDNFFKVVGQMILERDPLDEIKRAFKLFDEDNTGKISLRNLRKIAKDLGENLTDEELRAMIEEFDLDEDGEINEQEFIKICTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHW8</accession>
    <name>G3AHW8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Serine/threonine-protein kinase STE7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_135326</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373866.1">
        <property type="nucleotide sequence ID" value="XM_007373804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHW8"/>
    <dbReference type="FunCoup" id="G3AHW8">
        <property type="interactions" value="497"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHW8"/>
    <dbReference type="GeneID" id="18869884"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135326"/>
    <dbReference type="eggNOG" id="KOG0581">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_23_1"/>
    <dbReference type="InParanoid" id="G3AHW8"/>
    <dbReference type="OMA" id="SWAKKIR"/>
    <dbReference type="OrthoDB" id="10252354at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000196">
        <property type="term" value="P:cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071507">
        <property type="term" value="P:pheromone response MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001402">
        <property type="term" value="P:signal transduction involved in filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000040">
        <property type="entry name" value="Dual specificity mitogen-activated protein kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050915">
        <property type="entry name" value="MAP_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47448">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE DSOR1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47448:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE STE7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="204"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="144"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="406"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="157"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="406"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="520" mass="58095" checksum="2150A153C2B1E013" modified="2011-11-16" version="1">MMNWNNSSDTSLGSSKDKDLPPVPVFSSVTGSTKLDATDDSSELLQAKTLKRKNFKKLSLDASPSKDNARVIDDDSMIRTPISLRQRRQRPAPMLNQALTRNNSMPDSSVELNINKSNRNVDPDQETSADIIINQISNLDLNNKKQGSVTRKRQTVISSISPTKSTSSSPLEPRPASFATALNTIEDQSPIATTGSFELNTKDLVTLKSLGSGHSGTVTKVLHVPTEKIMAKKIIHIDSKSVIQRQIIRELRILHECHSPFIIDFYGAFLNTNNTIVICMEYCNCGSLDKILPLCENKQFPLIVLKKLAFAILSGLSYLYTTHKILHRDIKPNNVLMTHKGEFKLCDFGVSRELTNSMAMADTFVGTSTYMSPERIQGLNYGIKSDVWSMGLMLIELASGIPIWTDDDDDEDKEGEENGNFETRGPEGILDLLQRIVNEPAPTLSNKINPVTKTKYDGKLCQFIDLCLIKDDKSRGSPWQLLEDKNGFLQGVSEGVYDKEHKSWAKKIRKLSKEKNEQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI14</accession>
    <name>G3AI14_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">U3 small nucleolar RNA-associated protein 11</fullName>
            <shortName evidence="6">U3 snoRNA-associated protein 11</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_49381</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the ribosomal small subunit (SSU) processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Nucleus</location>
            <location evidence="2 6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the UTP11 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373912.1">
        <property type="nucleotide sequence ID" value="XM_007373850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI14"/>
    <dbReference type="FunCoup" id="G3AI14">
        <property type="interactions" value="751"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI14"/>
    <dbReference type="GeneID" id="18871329"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49381"/>
    <dbReference type="eggNOG" id="KOG3237">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061887_0_2_1"/>
    <dbReference type="InParanoid" id="G3AI14"/>
    <dbReference type="OMA" id="DLKYVVM"/>
    <dbReference type="OrthoDB" id="29058at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007144">
        <property type="entry name" value="SSU_processome_Utp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12838">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12838:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03998">
        <property type="entry name" value="Utp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015952">
        <property type="entry name" value="U3snoRNP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="224"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="239"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="248" mass="29296" checksum="41238EAE0C2A6C9C" modified="2011-11-16" version="1">MAKLVHNVQKKQHRERSQVSSRAKYGLLEKKKDYRLRAADYHKKQAALKALKEKAKLHNPDEYYHSMTKRHTDERGILIADRGNEELSNDQVKLLKTQDINYIRTMRLNEMKKIEKEKHGKLFEGSGKHTVFVDSVSEQQQFNPAEFFNTDESLVENRENRLKLDQLYVNSGVINKANVPEPELKDKIDEKKVKSFKLLQRRLKKEQELKHVESIMSAKLETMKKGNKKKMVDGEGKVHFKWKNQRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI74</accession>
    <name>G3AI74_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_59815</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373972.1">
        <property type="nucleotide sequence ID" value="XM_007373910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI74"/>
    <dbReference type="FunCoup" id="G3AI74">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI74"/>
    <dbReference type="GeneID" id="18873087"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59815"/>
    <dbReference type="eggNOG" id="ENOG502QT3R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061432_0_0_1"/>
    <dbReference type="InParanoid" id="G3AI74"/>
    <dbReference type="OMA" id="DQVTHGD"/>
    <dbReference type="OrthoDB" id="94039at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050228">
        <property type="entry name" value="Carboxylesterase_BioH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43194">
        <property type="entry name" value="HYDROLASE ALPHA/BETA FOLD FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43194:SF2">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN LPX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="51"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="369" mass="41555" checksum="4D9CEF8C5B5870CD" modified="2011-11-16" version="1" fragment="single">MSYTTEKKISSAHQFRAPGSTVLPEDAKDLKLVYFKYKTKIPVAGKLKFNLIFCHGTGFNKSVWTYYVNKLYSLCQTGSTSWAIDSVISIDQIGHGDSSLENEGKLGPIVRWDEGGKDVIEVIKHEIATTGDFTNDVNSRNVVIGHSMGGFNALYASFYEPTLIDACVPIEPVIYGEAGGLDKFKKIFGKIAKIMIDKFEDMQEVEFFFKEFSFYKNFHPQVLQDFMEDEIVAEVDPVTKEKKYRLKCKINNQMSAYLGAFMSIAIGMHILSLIRVPIFHVIGKKAVWNPPNSIPWIRSTINPEFLGESFDIPQGEHLVNGERPDEVISIIAKVLETRAEKFPAEAKRIPASSGSTRDQIAEDQFQKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIA1</accession>
    <name>G3AIA1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BPL/LPL catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_48797</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373254.1">
        <property type="nucleotide sequence ID" value="XM_007373192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIA1"/>
    <dbReference type="FunCoup" id="G3AIA1">
        <property type="interactions" value="132"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIA1"/>
    <dbReference type="GeneID" id="18871269"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48797"/>
    <dbReference type="eggNOG" id="KOG1536">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006150_1_1_1"/>
    <dbReference type="InParanoid" id="G3AIA1"/>
    <dbReference type="OMA" id="HHAFYSN"/>
    <dbReference type="OrthoDB" id="10250105at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin--[biotin carboxyl-carrier protein] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03144">
        <property type="entry name" value="GATase1_ScBLP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019197">
        <property type="entry name" value="Biotin-prot_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835">
        <property type="entry name" value="BIOTIN PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835:SF5">
        <property type="entry name" value="BIOTIN--PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09825">
        <property type="entry name" value="BPL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="373"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="675" mass="75023" checksum="2C2CDC75A2887113" modified="2011-11-16" version="1">MNVLVYSGPGTTTESVKHCVESLRFHLSSYYAVLPVSESVILNEPWMRKTSLLVMPGGADLPYCNVLNGNGNRKIASYVKQGGAYLGFCAGAYFAAARCEFEIGNPVMEVSGPRELAFYPDAKRGCAFKGFQYESHAGARAVQVSVNTSILPDCPSSVYHYYNGGGVFINASKYKDVEVLARYDERIDVDDIDKAAVVFRKVGKGRVLLTGTHPEFSYNLLKPFQHEHDDKFTTVIEKLKENDHDRKLFTKACLKKLGLRVSENVDASIPNVTPLIVASPVANIGQIYQDLKTNLDIKNGFFQDNNDVFVFQDESHEEYHVENERIDVEDPTKATKHVKFMTSGTIPTNKMTPYFNMNKYFEHLKELSKGNIGQYGNILGYAEVTTSTNTILEKNPHWLEHLPAGFTLTATTQIAGRGRGGNVWINPKGVLATSILFKISQSAKSSSSVVTMQYLCGLALIEAILGYGSTESGKGVGYEDMPLRLKWPNDIYILKPEYFNSLADKNDVSSTVEGDDEKYSKVSGALINSQYLNGQFYLVWGGGVNVSNPAPTTSLNLVLQKLNLIREKQGLPLLPHYEPEKLLAKLMHTVNSFFPVFEKAGLSPFLPLYYKRWFHSEQKVAVNGADGKPRTCVIKGITPDYGLLIAEDVNNHEVLHLQPDGNSFDIFKGLVYKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJB3</accession>
    <name>G3AJB3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SWR1-complex protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_149659</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SWC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374156.1">
        <property type="nucleotide sequence ID" value="XM_007374094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJB3"/>
    <dbReference type="FunCoup" id="G3AJB3">
        <property type="interactions" value="992"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJB3"/>
    <dbReference type="GeneID" id="18870832"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_149659"/>
    <dbReference type="eggNOG" id="KOG2656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018539_4_0_1"/>
    <dbReference type="InParanoid" id="G3AJB3"/>
    <dbReference type="OMA" id="GNTTMYQ"/>
    <dbReference type="OrthoDB" id="19740at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032563">
        <property type="entry name" value="DAMP1_SANT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008468">
        <property type="entry name" value="DMAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027109">
        <property type="entry name" value="Swc4/Dmap1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12855:SF10">
        <property type="entry name" value="DNA METHYLTRANSFERASE 1-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12855">
        <property type="entry name" value="DNA METHYLTRANSFERASE 1-ASSOCIATED PROTEIN 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05499">
        <property type="entry name" value="DMAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16282">
        <property type="entry name" value="SANT_DAMP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="10">
        <location>
            <begin position="260"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="145"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="145"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="598" mass="70135" checksum="7E8CF79806A8152B" modified="2011-11-16" version="1">MSANDILDVLNISRDEPPKKKQRNESTPQTQQPQLTGMARELYNLIGPNTPPLQLSSNNYTKTSIKDKINFKPSPWTKMEFNPKKRAREEDGLKLYHWTKGSKEFLESAEEVNKPYFFEKFNVNLSIPELVDEETYDKFMEEIQEHERKEKERKLLELKERERERQEKEKEKEKERQKAEAKKEEARKKQEAKEKEDQKKKDDKDKPEVVDIKKEDDKPEEKEKSDEKTEEDKADDQTEAREKSASKEQEAKQDEPIEDEELEWTYEETNYLFELCKAFELKWPIIYDRYNYNNVRTCEDLKEHFYRLCIKILQSKRDSSQSSLIESLKAYSKPREIERKQYLENLLKRTPAEIAEEESLVIEARRFELAAKKMLIERSNLLTLLDSPQSTQNSSQYQSSQGLANLYNNLMIFDKHQKKKQAQQLQLQQQKASEPIPPPIPMAASSSFKKDKVFQTHLQQYLSSLLKQNVHTNPAIKQEASAIQQMLSKRLTQKEEEAYGLHYHGNEKLTPGVVLRSTQKLPSLNQKQSTLKSVNQVLQELDIPTGGGTAWKPVMPTRKTMSRYDELIRTVVALLEVKKAKDKLESEINLIKSQRGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJF3</accession>
    <name>G3AJF3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60046</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374196.1">
        <property type="nucleotide sequence ID" value="XM_007374134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJF3"/>
    <dbReference type="STRING" id="619300.G3AJF3"/>
    <dbReference type="GeneID" id="18873195"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60046"/>
    <dbReference type="eggNOG" id="ENOG502RQKI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1185657_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJF3"/>
    <dbReference type="OMA" id="RSIYCEA"/>
    <dbReference type="OrthoDB" id="4026080at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="171"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="234" mass="23134" checksum="26B135D86595EA73" modified="2011-11-16" version="1">MARIHKRDACSGSCVDAAKTEVNKCRIGQSNPSDQDLVAEFNCICKLPDSFFNDLSDCIKNCDQVDQGGLDGFDSSPSGLRQLYCDSAASFSSLVQEFSGTLPTAYGGAAAGIGGGLGGFATQDVGAAETDTGVGGNGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTHTTNTNTHTTNTAGTTNGNGSAAKTDTSETSKTDTSTKETSHNAASTMGVCSIIYLVIFALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJR2</accession>
    <name>G3AJR2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_59374</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373547.1">
        <property type="nucleotide sequence ID" value="XM_007373485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJR2"/>
    <dbReference type="FunCoup" id="G3AJR2">
        <property type="interactions" value="979"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJR2"/>
    <dbReference type="GeneID" id="18872884"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59374"/>
    <dbReference type="eggNOG" id="KOG1692">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066963_4_0_1"/>
    <dbReference type="InParanoid" id="G3AJR2"/>
    <dbReference type="OMA" id="MQVRDRN"/>
    <dbReference type="OrthoDB" id="62956at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016050">
        <property type="term" value="P:vesicle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012565074" description="GOLD domain-containing protein" evidence="10">
        <location>
            <begin position="16"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="11">
        <location>
            <begin position="25"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW33963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="197" mass="22672" checksum="38014E2B3BB81CB7" modified="2011-11-16" version="1">MRYSIVLFLISLVTAHNVLLAPYGKQCFFENLKKNDELAISFQVGSRDPKNSEQYDVDFWINSPQGQTLVKRTGFDHGDESITAPSNGKYTYCFSNEKTGRVDLDVSFNIHGVIYIDSTDPKSDTLDYAIQRLSQLTNDVKAEQSYLVIRERTHRNTAESTNSRVKWWSVFQILVVAANSIFQIYYLKRFFEVQSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJU3</accession>
    <name>G3AJU3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1776-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59401</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373578.1">
        <property type="nucleotide sequence ID" value="XM_007373516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJU3"/>
    <dbReference type="STRING" id="619300.G3AJU3"/>
    <dbReference type="GeneID" id="18872901"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59401"/>
    <dbReference type="eggNOG" id="ENOG502QWSS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022136_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJU3"/>
    <dbReference type="OMA" id="HDVRRYS"/>
    <dbReference type="OrthoDB" id="5308060at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013952">
        <property type="entry name" value="DUF1776_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43313:SF1">
        <property type="entry name" value="3BETA-HYDROXYSTEROID DEHYDROGENASE DHS-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43313">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE FAMILY 9C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08643">
        <property type="entry name" value="DUF1776"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="441" mass="49633" checksum="F913F10857D4AFCF" modified="2011-11-16" version="1">MVAEPVQATVQTLKQLYSQATGFIDTQYDNVVNSDLYNNSYDKITKLNPFGETNGSIISIGNPPPPSPPPSPSHSAYGACSRMYQKVYDSINENRARYITLFSCVGLGGAAWLIYKRRKRNLSDSAEKRHKRRVPKLTNGARRDVVLIIGSPTEPLTRLIALDFEKRGFIVYLTILDENDSKYIESNPITDDVNYLNLNDKKGNMETHVSTFNQLLKLPVVPFPGAEAHTLNLVGVVFAPSLYFPIGPIENIAISSWAKLNDRVMTYLKLLSSGLIQLVRTQQAKLILVNPNILSALDMPYHAPESIFQNQLKHLFTTLTRELSSHGISVTQVRLGNLHISNQKLSSGPRIESLVNTEVRAWTSEMKQLYSDDFSRSQFKANPIKSTGGKGTSLRELYHLLFDLLYSEKRNPPVVYCGTGARGYDLICRIFPESWIEWFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJV7</accession>
    <name>G3AJV7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_65199</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373591.1">
        <property type="nucleotide sequence ID" value="XM_007373529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJV7"/>
    <dbReference type="STRING" id="619300.G3AJV7"/>
    <dbReference type="GeneID" id="18875025"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65199"/>
    <dbReference type="HOGENOM" id="CLU_1541070_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJV7"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="174" mass="19770" checksum="C360368AF4ED8010" modified="2011-11-16" version="1">MSRKVSHEIIEYKSCLVSFVVFAIFLGVLFWFNYDRGIRRNGGFESLGEISLDEAGTDEIGSDIIVSCVVKSGTFSLARVKTFFDQVVFERITLGCIPGRRNYVKVPDGEEEATLFGNHNKEFQDDSSLNKHTFHDEAVGLKHTGQRDPDVLDERLFDIESDNDVPEVAREEHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK22</accession>
    <name>G3AK22_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59500</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373657.1">
        <property type="nucleotide sequence ID" value="XM_007373595.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK22"/>
    <dbReference type="GeneID" id="18872944"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59500"/>
    <dbReference type="HOGENOM" id="CLU_2284261_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK22"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="102" mass="10486" checksum="BD7F7AAEFCAE878B" modified="2011-11-16" version="1" fragment="single">MTNKPPAAADAAAAAPVPPPAPPPPPRPESTTNDRAESPYPNHHKQQQQSSTIETATNATNATNTAATPVPSSQVQATQMNTSSSTTTVSSSNGKTQVVFIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK99</accession>
    <name>G3AK99_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60897</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375073.1">
        <property type="nucleotide sequence ID" value="XM_007375011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK99"/>
    <dbReference type="FunCoup" id="G3AK99">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK99"/>
    <dbReference type="GeneID" id="18873624"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60897"/>
    <dbReference type="eggNOG" id="ENOG502SBSE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_713702_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK99"/>
    <dbReference type="OMA" id="PWVQPCT"/>
    <dbReference type="OrthoDB" id="2152029at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019436">
        <property type="entry name" value="Say1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF31">
        <property type="entry name" value="STERYL ACETYL HYDROLASE MUG81-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10340">
        <property type="entry name" value="Say1_Mug180"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="392" mass="44798" checksum="AE67920E45757C42" modified="2011-11-16" version="1">MMSADFFLKVVTIPYIVARITFEYYTYGTPYSKTNNEFKNSLYKNLLLAIEYHVCGNYKKQDMKLVCYQPIEDLFAKYKSSLLTAQLNNFGTKFDEHGYWIHQAETKDNVLVYLHGGGYLLNLFDCQLVFAGALHYALDDHTQTNTSIMVLDYSLTMFDKVYPTQLYEALVNYKNLVVGGYKSISYIGDSAGTHLALGVARSLAYPHEIISQFTNFPEFNFSADFFKLNEQEQLPQPKSLILISPWVQPCTPPLVPNRRGINTWGDLGAEDITLGNFFLGNNNIHLINNFFTFTNTNFDEHWAKVEPINNGNTMIIVGEREVLRDSVDDLYEIINKLGTIEYYVEEGGIHAGLVYVESLDYYGDSGSKKAIRGDFNNKYAFNLVADFLSRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKA6</accession>
    <name>G3AKA6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_60903</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375080.1">
        <property type="nucleotide sequence ID" value="XM_007375018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKA6"/>
    <dbReference type="FunCoup" id="G3AKA6">
        <property type="interactions" value="231"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKA6"/>
    <dbReference type="GeneID" id="18873628"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60903"/>
    <dbReference type="eggNOG" id="KOG0752">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_10_0_1"/>
    <dbReference type="InParanoid" id="G3AKA6"/>
    <dbReference type="OMA" id="VYERMKW"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015228">
        <property type="term" value="F:coenzyme A transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000151">
        <property type="entry name" value="LEU5p Mitochondrial carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="54"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="153"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="319"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW33565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="413" mass="46862" checksum="AFE569D670C2DE5E" modified="2011-11-16" version="1">MVKIRNSDPSPSSIESIPPIHEEPYELVPAPGHYSNDQSTPIEDVRIINKQSLEYVLISGIAGGAAGSAAKSLVAPLDRIKILFQTSNPEFLKYRGTSHGLFLAGRQIWRNDKLWGLYQGHSVTLLRIFPYAAIKFVAYEQIRTLLIPNDNYETAARRFMAGSLSGLMSVFFTYPLDLVRVRLAFETRNLTKTHLQLHPEHSQFMKRHQGRLASTINMIYHENPPPKINDPGWLKYMRDTFPPQIQKISNFYRGFAPTIMGMIPYAGVSFYTHDLLHDILRSKYLAKYTVQQHVHRKVQIVNKNKQAGTKSSRESRAPLTTWAQLVAGGLAGMCSQTAAYPFEVIRRRMQVGGAVNQGQFVGFKSTAKLIYRESGIRGFFVGLSIGYMKVIPMVSCSFFVYERMKKFVSRRFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKP1</accession>
    <name>G3AKP1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mitochondrial inner membrane protease ATP23</fullName>
            <ecNumber evidence="8">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_135711</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Has a dual role in the assembly of mitochondrial ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion inner membrane</location>
            <topology evidence="1 8">Peripheral membrane protein</topology>
            <orientation evidence="1 8">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the peptidase M76 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="8"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374460.1">
        <property type="nucleotide sequence ID" value="XM_007374398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKP1"/>
    <dbReference type="FunCoup" id="G3AKP1">
        <property type="interactions" value="491"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKP1"/>
    <dbReference type="MEROPS" id="M76.002"/>
    <dbReference type="GeneID" id="18869912"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135711"/>
    <dbReference type="eggNOG" id="KOG3314">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079125_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKP1"/>
    <dbReference type="OMA" id="EAHQNCV"/>
    <dbReference type="OrthoDB" id="285308at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019165">
        <property type="entry name" value="Peptidase_M76_ATP23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21711">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21711:SF0">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE ATP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09768">
        <property type="entry name" value="Peptidase_M76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4 8 9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="239" mass="27599" checksum="0F84759522828B22" modified="2011-11-16" version="1">MSQDKPAETTGNNLKLSPQDALSGFSWWTRTLQYATGLGISDQEKLQYEYDYQNRNLDEKCVQCNEYRDWMLTYSPSVTFMMSHINKLTKDNRPALDKSNVVCDVCDDLKGGGFHPDEGILLCANWLRSRSQLEDVLTHELVHAYDYLRFNIDLTDLRHHACTEIRASMLSGECRIMNEIKKTGLGNFGRKFQDCVRRRAVLSVSANPKCTSKEEAEKVVNTVWNSCFNDTRPFERVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKZ1</accession>
    <name>G3AKZ1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flo11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_66046</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374549.1">
        <property type="nucleotide sequence ID" value="XM_007374487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKZ1"/>
    <dbReference type="STRING" id="619300.G3AKZ1"/>
    <dbReference type="GeneID" id="18875077"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66046"/>
    <dbReference type="eggNOG" id="ENOG502S91K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_274765_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKZ1"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018789">
        <property type="entry name" value="Flo11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10182">
        <property type="entry name" value="Flo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51824">
        <property type="entry name" value="FLO11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442410" description="Flo11 domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="domain" description="Flo11" evidence="3">
        <location>
            <begin position="28"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Flo11" evidence="3">
        <location>
            <begin position="210"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="380"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="926"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="434"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="470"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="545"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="555"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="592"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="648"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="951"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="985"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1018"/>
            <end position="1029"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1165" mass="128225" checksum="5FE580D7A128702D" modified="2011-11-16" version="1">MQLSLFLYYFILQLPFTISALQVKRASSDNPTTLTWPAPFSYEKEDCPFYWGDRCPQGDNYPKPDWAPKIKTFQYVYLNYVQDREDLYEVMIEFELYTDNLPAGDLVSITFNNLRTPEDYIPNPQSALANWLDASESVSSSPYHFFVKWVMQAEYNGYQLCTTPFSIKYSWTPQIPSFKRALAVPNAVVEYVHDCYGEYPIQCWDQICGKKVNAEPSTITSTLANLPQPTDCPDSYEPMMCNYQGSGDGLEIFRFVSVHHISDLLYEVTIEFQLKDSLYPVRDLQEITIDQLTTPGNYLPNLQLYGNGMDPVGDSPYHWFFTFVMEAEDMGNFVCATPFSIYYQWSGFSKTYIHSCGELLDGIYDLPLQCWENTCVKPLQETTTESEKESSTEPIEIELTESSEETSTEETSTENEEDESFTEPEKDYSTKLQSESSTESGEGTFTESNQDSSSSEDFSDPSSEIITESWFEQVSTPYPTSELTAISSEPGTDISFSNIPTQLPFPIESPIVHPENPIESNSEDLTDPNTNEPTDPMTREPMDPGADDPIEYITEESTVTPTYEHSYPSTGETNILSTDENSYPPTEEQTDESTIPPTTDMSQPETKTTEEPSITYNTNQTGTEEPTSLVSKGLSETYSEDPPELHSESSTVLPTEEPTLSENPTIPILGEVTFTVIQSSDQPPPPSIETVTEANAETNINPTIRTFPESFTSVNSDPPPVTITLTEIITTTSIDPSNPNVSETLILTVITTSIDVPQVVVTTTVSRPDTTYVTTITEPYNPPVIVTTTVSESDSSYVTTITEPYNPPVTVTVSNPDTTYVTTVTNPYYPPPTVTTIVNPDTTYVVTVTPPYVPPGVVTTITNPYTTYVTTGPYNPPVVVTTIVYPDTTLVATVTGPYVPPPFVTTVTGANTVYVTTISYPPVYPPVPTGSQPGGTTPGNVNLEQGEELQTPPSGGNSYAPANSNDSSANLEQNEELQVQYPDANTNSPSSSNTSQGAPNVPANFNPTEDVSRPENPNDPTTNSNQTEELQAPVSGDDPNSTENANAPVGNPNDPSLQNDEELIQDTKVFSYTSAVSTTIVSHGTTYETSIPVIYTTTVPQNEIPDGIPEVLVATANPTSPDYDDTSNANPDLEELLEGFEGLASDISINTFMLCLCLISTIIFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL02</accession>
    <name>G3AL02_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">60S ribosomal protein L17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_60363</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374560.1">
        <property type="nucleotide sequence ID" value="XM_007374498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL02"/>
    <dbReference type="FunCoup" id="G3AL02">
        <property type="interactions" value="1045"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL02"/>
    <dbReference type="GeneID" id="18873366"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60363"/>
    <dbReference type="eggNOG" id="KOG3353">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083987_0_0_1"/>
    <dbReference type="InParanoid" id="G3AL02"/>
    <dbReference type="OMA" id="NTYETAR"/>
    <dbReference type="OrthoDB" id="10254664at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.10:FF:000010">
        <property type="entry name" value="60S ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="entry name" value="Ribosomal protein L22/L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018260">
        <property type="entry name" value="Ribosomal_uL22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005721">
        <property type="entry name" value="Ribosomal_uL22_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_uL22_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01038">
        <property type="entry name" value="uL22_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593:SF10">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="Ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00464">
        <property type="entry name" value="RIBOSOMAL_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW33045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="185" mass="20854" checksum="7BAD8ACD00F22F7C" modified="2011-11-16" version="1">MVRYAATPANPAKSASARGAYLRVSFKNTRETAQAINGWKLEKAQKYLDQVLDHQRAIPFRRYNSSIGRTAQGKEFGVTKARWPAKSVNFVKDLLRNAQSNAEAKGLDSSKLVISHIQVNHAPKQRRRTYRAHGRINAYQSTPSHIELTLTEEDEAVEKAAETKKVRLNTRQRGRLASQKRLTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL28</accession>
    <name>G3AL28_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Agglutinin-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_136382</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ALS family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374586.1">
        <property type="nucleotide sequence ID" value="XM_007374524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL28"/>
    <dbReference type="FunCoup" id="G3AL28">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="GeneID" id="18869963"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136382"/>
    <dbReference type="eggNOG" id="ENOG502RGCG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031316_2_0_1"/>
    <dbReference type="InParanoid" id="G3AL28"/>
    <dbReference type="OMA" id="RINGNDM"/>
    <dbReference type="OrthoDB" id="3981162at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903561">
        <property type="term" value="C:extracellular vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030446">
        <property type="term" value="C:hyphal cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030445">
        <property type="term" value="C:yeast-form cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044403">
        <property type="term" value="P:biological process involved in symbiotic interaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030448">
        <property type="term" value="P:hyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2430">
        <property type="entry name" value="Agglutinin-like protein, N-terminal domain, N2 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008966">
        <property type="entry name" value="Adhesion_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008440">
        <property type="entry name" value="Agglutinin-like_ALS_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024672">
        <property type="entry name" value="Agglutinin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043063">
        <property type="entry name" value="Agglutinin-like_N_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033504">
        <property type="entry name" value="ALS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011252">
        <property type="entry name" value="Fibrogen-bd_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793:SF2">
        <property type="entry name" value="AGGLUTININ-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793">
        <property type="entry name" value="ALPHA-AGGLUTININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05792">
        <property type="entry name" value="Candida_ALS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11766">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01056">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49401">
        <property type="entry name" value="Bacterial adhesins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0134">Cell wall</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 17" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0843">Virulence</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442477" description="Agglutinin-like protein N-terminal domain-containing protein" evidence="17">
        <location>
            <begin position="18"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Agglutinin-like protein N-terminal" evidence="18">
        <location>
            <begin position="53"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="436"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="455"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="19">
        <location>
            <position position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW33071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="473" mass="51205" checksum="D0F2809EE6729514" modified="2011-11-16" version="1" fragment="single">MLSFIIFIILTIATVSSEQISGVFTSFDSLVWEKAADYPFAGPSYPSWIVKLSWRINGNDMNAGDTFTLDLPCVFKFTTNQASVNLNVGKTNYATCAFNPGDIVVAFSKLECVLLDTVTSSTNAHGSINFPVAFNVGGSALSTDLESSTCFVDGRNTVSFYDGDNQLSTQVSFSGGATDDPNKIVYRNRVVPSLNKQQHYLLAGNCRARYKSGSIGMKIQNQGSKINCDSIHMAITNSLNDWYLPKNANNDFKFTYTCTSTSFMVEYKNIPAGYRPFIDSLFDVANGVSVSVNYVNNYICADSTKTTDNSKSINWSAYENNVVGGNGEEVEVVTSTYTGSTTQISTMPFQTSKDKTMTIVVNVPIPTVTTTTTYVGISTSYTTITAPPGSTASVIEFEPVYTTTTITTCWVGEEATFTTTYSTSTWATVTEAIVTPCPSPSASEESTFSEDLLTEVSSTTNDPSTTEDPVSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL39</accession>
    <name>G3AL39_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_70994</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.n1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the GTP-binding elongation factor family. LepA subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374597.1">
        <property type="nucleotide sequence ID" value="XM_007374535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL39"/>
    <dbReference type="FunCoup" id="G3AL39">
        <property type="interactions" value="724"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL39"/>
    <dbReference type="GeneID" id="18875354"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70994"/>
    <dbReference type="eggNOG" id="KOG0462">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009995_3_1_1"/>
    <dbReference type="InParanoid" id="G3AL39"/>
    <dbReference type="OMA" id="QVKCDEN"/>
    <dbReference type="OrthoDB" id="1074at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097177">
        <property type="term" value="F:mitochondrial ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01890">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03709">
        <property type="entry name" value="lepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000078">
        <property type="entry name" value="Elongation factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000015">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000007">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2570:FF:000001">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000004">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2570">
        <property type="entry name" value="Elongation factor 4, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00071">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006297">
        <property type="entry name" value="EF-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038363">
        <property type="entry name" value="LepA_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013842">
        <property type="entry name" value="LepA_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035654">
        <property type="entry name" value="LepA_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01393">
        <property type="entry name" value="lepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512:SF7">
        <property type="entry name" value="TRANSLATION FACTOR GUF1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512">
        <property type="entry name" value="TRANSLATION FACTOR GUF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06421">
        <property type="entry name" value="LepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="35"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="44"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="108"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="162"/>
            <end position="165"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="631" mass="70306" checksum="94AC7502E9537723" modified="2011-11-16" version="1">MLTINTKSVEEKKQILNKEAYRKSIQDRIAKIPIENYRNFSIVAHVDHGKSTLSDRLLELTGVIQPGSNAQVLDKLDVERERGITVKAQTVSMIYNRQGQDYLLHLVDTPGHVDFRAEVSRSYASCGGALLLVDASQGVQAQTVANFYLAYSMGLKLIPIINKIDLDSADIPRAMDQVEGTFELPREDCIAVSAKTGLNVEQIIPSVIDNIPSPEGDASKPLKALLVDSWHDTYVGVVMLVFVVDGKLKKGMKILSAHSNNTYEVKEVGIMYPDRMPMDEIKAGQVAYVIPGMKNPREALVGDTFFQSGKSQGLEPLPGFEEPKPMVFVGAFPAEGVEFKVMDDQMQNLVLNDRSVHLEKETSNALGLGWRLGFLGSLHASVFKERLEKEYGAKIILTAPTVPYKVVYKDATEEIVSNPDQFPDVNERAKVDCFMEPYVEAIMTLPGEYLGNVLTLCLNNRGIQKDLEYLNTGQVLLKFEIPTAQLVEDFFGSLKGCTKGYASLDYEEAGYKKSDIVKMELCVNGIAQDALTTIVHRDQAQSKGKEYVSKFKKYLKMQLFEVAIQAKVGGKVVARETVKARRKDVTQKLHAADISRYKKLLERQKEGKKQMKADGRVSIGNDAYQAFLRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL54</accession>
    <name>G3AL54_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">4-hydroxyphenylpyruvate dioxygenase</fullName>
            <ecNumber evidence="8">1.13.11.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">4-hydroxyphenylpyruvic acid oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">SPAPADRAFT_150722</name>
    </gene>
    <organism evidence="26">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="22">Catalyzes the conversion of 4-hydroxyphenylpyruvic acid to homogentisic acid, one of the steps in tyrosine catabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>3-(4-hydroxyphenyl)pyruvate + O2 = homogentisate + CO2</text>
            <dbReference type="Rhea" id="RHEA:16189"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16169"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:36242"/>
            <dbReference type="EC" id="1.13.11.27"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:16190"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 3/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the 4HPPD family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.27" evidence="8"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374612.1">
        <property type="nucleotide sequence ID" value="XM_007374550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL54"/>
    <dbReference type="STRING" id="619300.G3AL54"/>
    <dbReference type="GeneID" id="18870878"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_150722"/>
    <dbReference type="eggNOG" id="KOG0638">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034004_3_1_1"/>
    <dbReference type="InParanoid" id="G3AL54"/>
    <dbReference type="OMA" id="DPFPVKG"/>
    <dbReference type="OrthoDB" id="414569at2759"/>
    <dbReference type="UniPathway" id="UPA00139">
        <property type="reaction ID" value="UER00362"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003868">
        <property type="term" value="F:4-hydroxyphenylpyruvate dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07250">
        <property type="entry name" value="HPPD_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08342">
        <property type="entry name" value="HPPD_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.180.10:FF:000022">
        <property type="entry name" value="4-hydroxyphenylpyruvate dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005956">
        <property type="entry name" value="4OHPhenylPyrv_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041735">
        <property type="entry name" value="4OHPhenylPyrv_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041736">
        <property type="entry name" value="4OHPhenylPyrv_dOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01263">
        <property type="entry name" value="4HPPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959">
        <property type="entry name" value="4-HYDROXYPHENYLPYRUVATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959:SF1">
        <property type="entry name" value="4-HYDROXYPHENYLPYRUVATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15 25" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="17" id="KW-0408">Iron</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="25" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="domain" description="VOC" evidence="24">
        <location>
            <begin position="101"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="VOC" evidence="24">
        <location>
            <begin position="251"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00018452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00029786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00033727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="EGW33097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="475" mass="54436" checksum="1D4F77C42B1C607E" modified="2011-11-16" version="1">MSKYLQLSMGFTEIAYRGLENDSRLVGSHVVRNGGVIFEIVNTLETVEDDNVLKFPYFQHDLAKFQEINKQDHLDKFKVTTSDLIFDFVNNRIESFANSSRSFKLGRNIYNKISASRSLQNTMNEFILNTINSSETIYNDIMECTLIQKFLKKHGEGVMDISFQVVDVDYIFNRAIQAGAGIIRLPKVLHDNNGKVKLATISIPNTDIQHTLVQLIDYTGPFLPGYFNPVMDFSTSFQYQLGLLPPVNLECLDHCVENYASNQMIPQVKFYAEIFGFHKFWSVDESDISTENTSLRSTVMASSNGRIKMPINEPGQSKMKSQIQEFNDFNGGPGVQHIALKTNDIIGTVSALIKRGIEFNVAAKNYYPTLERRLNNDDISLYEDFQELKKYNILVDYDYSTRNNKSKRCNYLLQIFTKPLHDRPTLFIEIIQRHHHNGFGRGTFKGLFESIEEQQKIRGTFVEVSPRSGIFKPPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL68</accession>
    <name>G3AL68_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_60424</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374626.1">
        <property type="nucleotide sequence ID" value="XM_007374564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL68"/>
    <dbReference type="STRING" id="619300.G3AL68"/>
    <dbReference type="GeneID" id="18873399"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60424"/>
    <dbReference type="eggNOG" id="ENOG502S704">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074390_1_1_1"/>
    <dbReference type="InParanoid" id="G3AL68"/>
    <dbReference type="OMA" id="INIIDDW"/>
    <dbReference type="OrthoDB" id="20681at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1230.10">
        <property type="entry name" value="MTH938-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036748">
        <property type="entry name" value="MTH938-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007523">
        <property type="entry name" value="NDUFAF3/AAMDC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21192:SF2">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX ASSEMBLY FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21192">
        <property type="entry name" value="NUCLEAR PROTEIN E3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04430">
        <property type="entry name" value="DUF498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64076">
        <property type="entry name" value="MTH938-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="194" mass="21580" checksum="1811EBCADA3E425A" modified="2011-11-16" version="1">MFRQTIIRRFCTTPAYLNKGFGAKSLKPHAKQILGNPGNKQPTSNPADMFKRNDVLMYSDKPINYIESVKPDGFHFFNTMLITSPDKQGKNVGAILLGTEVYEVNLDNLEIDNVRVNFPSEVLKIFKKVYPKPEIVVIGLGKQNRILMPETKNVFSELGIQTEVGDSSSAASVFDLLATERPNVIGALLLPPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALG1</accession>
    <name>G3ALG1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60533</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374719.1">
        <property type="nucleotide sequence ID" value="XM_007374657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALG1"/>
    <dbReference type="STRING" id="619300.G3ALG1"/>
    <dbReference type="GeneID" id="18873454"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60533"/>
    <dbReference type="eggNOG" id="ENOG502ST6B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030189_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALG1"/>
    <dbReference type="OMA" id="ITNHEYI"/>
    <dbReference type="OrthoDB" id="4093693at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="429" mass="47614" checksum="D5E8A385823F5EED" modified="2011-11-16" version="1">MAHPGRPRGRTNKKTQQALAAQAQAQAQAQAAARAQAAQAQAAAQAQAAQAQSHIKEDPGGQLHDEADLVSFRNDALIRYITNHEYIENVMAKLVHTCKIIPPSLYPNVPKRTPEEIAKLRPEDTYFGDLESMKLAEKKLADDVTRLKDAEAQPFANYLLNDKEYHFQREKVDRLVEIQASLYNKESLQNLENEIDQILGEYQTKYKRKYYVTPSVKKYSVPIDQITPGVAVHTAPSNYNPKSITSIINLHNHPHEANNNGTLVENMSDNAMRIDSGMEEEPDIDTGKFNGVEGMFMNQFDNGPTNFNGNMSAHNFAMSMLSSHENEEDMPTSNGLASGDVNDDVDLSGDQNDSMAGGNVDDDNDQRDAIVDDDMSDLFKDNGDDLDPNQAIVNDDIGDLYFNDGDDNGLMGGSEFEQDFLSQIDHSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALI1</accession>
    <name>G3ALI1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_60561</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetate uptake transporter (AceTr) (TC 2.A.96) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374739.1">
        <property type="nucleotide sequence ID" value="XM_007374677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALI1"/>
    <dbReference type="STRING" id="619300.G3ALI1"/>
    <dbReference type="GeneID" id="18873465"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60561"/>
    <dbReference type="eggNOG" id="ENOG502QUJS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051062_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALI1"/>
    <dbReference type="OrthoDB" id="3648309at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015123">
        <property type="term" value="F:acetate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051633">
        <property type="entry name" value="AceTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000791">
        <property type="entry name" value="Gpr1/Fun34/SatP-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038013">
        <property type="entry name" value="AceTr_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123:SF1">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01184">
        <property type="entry name" value="Gpr1_Fun34_YaaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="260" mass="28186" checksum="72601E13F3229A27" modified="2011-11-16" version="1">MSNDKPVTATSSTSSVPYRTCSLTHEGDFVVVGNERHYKEEIEKQPLPPAPIYGNAGAVALIATSFNGLVTGFYLAGTRGATFLNAAVGLMFFYGGLVQFLSGIWEMFLGNTFAATMFTSFGCWWIQLGATFVPSFGIRAAYGDDEEMFNNAMGLLILGWGMFAFMMFSLTLKANFSLSFAILTLALTLILTSAGFLCGANPHIFKAAGIMSIINCFAGWYDAFGALYNRSNSYVLLPTFPIPVLHGHERVPGHGKCAEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALN2</accession>
    <name>G3ALN2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">mRNA 3'-end-processing protein RNA14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_151132</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="4">Nucleus and/or cytoplasm.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374790.1">
        <property type="nucleotide sequence ID" value="XM_007374728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALN2"/>
    <dbReference type="FunCoup" id="G3ALN2">
        <property type="interactions" value="1219"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0180010">
        <property type="term" value="P:co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="Rna14-like"/>
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        <property type="entry name" value="Suf"/>
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    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19980:SF0">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19980">
        <property type="entry name" value="RNA CLEAVAGE STIMULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05843">
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    </dbReference>
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    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="2 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Suppressor of forked" evidence="7">
        <location>
            <begin position="17"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="619"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="363"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="620"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00026188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="715" mass="82758" checksum="55062566AF0E7E5E" modified="2011-11-16" version="1">MPSNMFIQQTKKRLTLDKIGQLEEDLESDPLNYIKWNKLIEQVIAKDNQEQVRAVYTKYLSIFKFDGPSWCKYIRYELNRGEKQKVESLFQQCFAITDSVELCRLYVDYVRSAADFITGGEQARGTVIQAFEFAIGKVGIDINSDGLWNDYLGFLKSWTPSANWEQQQKVDLVRKVYKKYLVIPTENIETSWSQYTKWENELNPATAQKFISEKSAEFMLARSWHTEWNNLTEKKLKRTVSPYSVVGEHQETIHHQLNLWLKWIELEKKNTLEIKDETLIEKRISYVYKQATYALPFVPQLWFTYSKYLLFNNEEANLGACIALLKDGASLNPKSMLLSFQLAELYEKDGSFDNAKETFNDLINALTNEFNGVKSQINELNEQLKQKTEDDNEENSETTEKKEFKISLADSKRLVKLEEIQQRLADSITLAYIKLMVASKRARDIKEARNVFKTARKFENIGYEIFVESALLEHYTGNPKTALKIFDLGKKFFSTNGKFLLAYLDYLIMINDAEAMRSTLQTSDTSISKEISSISESLALGDLDPITKERKEKELKIKKEYLRKLFKKYISFAADYLQMDVTNSFVKKYEQLLPEDDPIDLFTDRYKLGQSNLIKKTEIGGDEYSDNEDEEPRTKKRRVSRKAQEEVNTVVQNIQESTTQITEQETKPQDSFVGPSIIALMGALPNASYFGLPSESVFDSEKLVTLFANLSNIPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALW5</accession>
    <name>G3ALW5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Autophagy-related protein 27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">SPAPADRAFT_60081</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus membrane</location>
            <topology evidence="4">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Preautophagosomal structure membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ATG27 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374239.1">
        <property type="nucleotide sequence ID" value="XM_007374177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALW5"/>
    <dbReference type="FunCoup" id="G3ALW5">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALW5"/>
    <dbReference type="GeneID" id="18873212"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60081"/>
    <dbReference type="eggNOG" id="ENOG502QVJJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047751_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALW5"/>
    <dbReference type="OMA" id="NKGNAID"/>
    <dbReference type="OrthoDB" id="29460at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018939">
        <property type="entry name" value="Autophagy-rel_prot_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071:SF13">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09451">
        <property type="entry name" value="ATG27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0072">Autophagy</keyword>
    <keyword evidence="17" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19" id="KW-0732">Signal</keyword>
    <keyword evidence="8 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442462" description="Autophagy-related protein 27" evidence="19">
        <location>
            <begin position="20"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="198"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="20">
        <location>
            <begin position="20"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EGW32724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="273" mass="30618" checksum="8044A94133064DA1" modified="2011-11-16" version="1">MLSITAFSILTTLVATCAGIDCSVKELKPYNFESIKGVHTLTTLKNTPPSQTNITWNIGICEPINSIEDCPKNSDLCGITSIILSDNKDKPIISEIVSFNANLQKTYKPFSKDDKDSDSDGIIISYSGVNWGDSLVDAELKFICDKKAEDDKFKLDKWDGTKLKLTMSTKAACITNDKDKKKNKPDDNKKKPDNGESWGWFTWIFIFLVLFLSIYIVGGAWFQYNKGNAIDFQSALKEVLENFIDLLRGLPGFCREIIERFTGNRSRGDYSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN17</accession>
    <name>G3AN17_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_67046</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375707.1">
        <property type="nucleotide sequence ID" value="XM_007375645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN17"/>
    <dbReference type="STRING" id="619300.G3AN17"/>
    <dbReference type="GeneID" id="18875135"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67046"/>
    <dbReference type="eggNOG" id="KOG1550">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006122_0_0_1"/>
    <dbReference type="InParanoid" id="G3AN17"/>
    <dbReference type="OMA" id="KMAMLYL"/>
    <dbReference type="OrthoDB" id="272077at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051726">
        <property type="entry name" value="Chitin_Synth_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430:SF3">
        <property type="entry name" value="ACTIVATOR OF C KINASE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430">
        <property type="entry name" value="PROTEIN SKT5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="391"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="459"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="515"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="557"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="33"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="391"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="461"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="522"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="561"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="575"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="605"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="660"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1208" mass="136200" checksum="83C34282ACF73ECB" modified="2011-11-16" version="1">MGLFSRFRSSHSRDSSVKSASKDHMRTDSFNGSVFSSATSDSSSTSAESMAYNKHNQLKPKRSAGLLSKSNANSAASSAWKSRPKSVFAGPSDKTAKPRPLSSINLDTASSHQSSSRSASPRRTRDSTLLTARKPPPADNIEPLPPHQSLDPKARSRSRSPGRTLEFETPQKQIIAQTIDVPAGNNIPRKSSPLAKVVYSPSPVIPPPVVSQKEGSLSPMSPGQPIGGGVNDSQLSLMLSPVSESVTSMSDPPLGNNNQFNFNQPAYVYHKPSTTTQDSYEADEERASSTSTVPPTQHPYYEQWKQYYEQLTLHQQQYMNSNPSLQGNASPQDANFNPMMFMNPMNMPPYFYNPFMQMQPPPSPTASNNASFPQLPLNYSNSHLFDPYMLQMQQQQQQQQQQQQQQQQQHHHHYQQKPGMPGRSSSAGNLVLEHDDNTEAQHDYLSQYKKTRNMKNELISEDNSLVSNSRKSTLKSNRYPSAPFQSRNVVSESRGRVASVDTNFKLNEFHIYEEDEEDSKNTTEASIPAANTKSSPLKSVEDRRRDLSRHISDYNKFLFGNEEDEENDSEEEEAEPKKDMEKVTSVDHQLPTPTSEDGLSRNDSESSATSSCDSIQDSIQSNGSKKFIVGDLKSKKSATSVIPRETSASPDGKSKKKVSPVSTQSFPLQNSSSRSDLNSGAPSPRPLSMMPSRSDLTGTVTPTPRPLSMMPQISRSMEPMYNSPYSNPVLTQNTEYSGSSVSLGHNRRQSIATAEAKRRSMAFNPNMFRDPNMGGNKRNSVAVFGSYSPPHQQQQHHQLQAPAKINDVTISKKIEQFIKLRKKIASGQKSLEYRLYWVKMLIDATNFKLYAYINIKGDPVSPEQIPNNKLLFMKSSETHLNKLMKELEADVIEREGIKSEVYYIYGSLFKQDYLNLYNQDFGIEKDVESAIMYFEKVLEINPKDFRALYKLGEIYEFDLENEFDRGVQYYTEAAKLGYNRAIFKMSMLYLHIPQLRSVKYLKYLHNLANIELRDVRLDEEDLDEMEEIIGLASYELGRIYEGIYPGDLSMDDDFIKDSVELAPVNYAKSLTYYNKSARLNCLLAQVRLGIVYEKGELNRQQNPNKSIQWFIKASSSPLSFRRHPEAMVGLARWYLRGTDGSSKYVPIASPEKAVMWCDRAIKEFNSPDALFMMGELSERRYTNSNPQLWYKKAYELGSEAAGEKLGYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN38</accession>
    <name>G3AN38_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">sphingolipid C(9)-methyltransferase</fullName>
            <ecNumber evidence="14">2.1.1.317</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_61519</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CFA/CMAS family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.317" evidence="14"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375728.1">
        <property type="nucleotide sequence ID" value="XM_007375666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN38"/>
    <dbReference type="STRING" id="619300.G3AN38"/>
    <dbReference type="GeneID" id="18873933"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61519"/>
    <dbReference type="eggNOG" id="ENOG502QS47">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026434_5_0_1"/>
    <dbReference type="InParanoid" id="G3AN38"/>
    <dbReference type="OMA" id="GFKTWLF"/>
    <dbReference type="OrthoDB" id="412182at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052290">
        <property type="entry name" value="Sphingo_C9-MT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45197:SF1">
        <property type="entry name" value="SPHINGOLIPID C9-METHYLTRANSFERASE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45197">
        <property type="entry name" value="SYNTHASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G04190)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02353">
        <property type="entry name" value="CMAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW32452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="512" mass="58555" checksum="AE7F69EAAC9426F5" modified="2011-11-16" version="1">MLQDVQFITTPEAKPQPAASNECGVWTSDSPSIHNAPLPADGPGSTHFSNLALLGFLTLIPAYIVRKIGLGFKSWVVLALLLAVPILMAYWTVLSTYSPRINEKVKYPNRPISYYLEYHTDELKAKYETSNGGKGKKIPIETFQELFFEGKVSFKGDCLDVLEYKHDWATFQFTLSLFRFFLLGMIPEVIMHSKSQDEEQVREHYDRGDDFYTWFLGPRMIYTSGVISDITKEESLEQLQDNKLKIVADKIDLKEGEHVLDLGCGWGTWATYASSQYGAKVTGITLGKNQTKWGTTLLKDYGVDSDQSRIVCVDYRDAPKSSKANGKYDKITCLEMAEHVGIRRFTAFLQQVSDSLEDDGLFYLQYAGLRKNWQYEDLNWGLFMNKYIFPGADASTPLSFVASCLESVGFEIVSVDNIGVHYSATLWRWYRNWIGNKDKVVNKYGVKWYRIWEFFLASSTVASRNGTATCYQFVLRKNLNSYRRVNYIPKQKGLLAPVQEGTQWAKEYNNFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN95</accession>
    <name>G3AN95_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Palmitoyltransferase</fullName>
            <ecNumber evidence="13">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_153465</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 13">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DHHC palmitoyltransferase family. AKR/ZDHHC17 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="13"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375754.1">
        <property type="nucleotide sequence ID" value="XM_007375692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN95"/>
    <dbReference type="FunCoup" id="G3AN95">
        <property type="interactions" value="629"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN95"/>
    <dbReference type="GeneID" id="18870956"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_153465"/>
    <dbReference type="eggNOG" id="KOG0509">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012510_1_0_1"/>
    <dbReference type="InParanoid" id="G3AN95"/>
    <dbReference type="OMA" id="GITHILH"/>
    <dbReference type="OrthoDB" id="6781668at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161:SF85">
        <property type="entry name" value="PALMITOYLTRANSFERASE HIP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 12" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="371"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="9"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="46"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="14">
        <location>
            <begin position="262"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW32478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="541" mass="62101" checksum="7CC4496DFE36A902" modified="2011-11-16" version="1">MYVDEPDGFNRTALHWAAYQGDTFTVNALLKFGADVSKVDNTLFIPIHWAFMKGYKVVLKTLLKAGSDIHFKNDQGKDTFEIAKDMNCVSTWTKVLTECDRDPKNNWAPRKRFINPKVGKLITFFTPYVILPIVLNICSVSAGFGIPKLFLGFGIIAVAVFTLNKLVVPTYLIDDKAIQKSPIFAGIFSATAFWAVMVWTYAMLPITFLNHFFLNIFLGGCIGLFSWAFFKSMFINPGFVPTPADNSVILSQVRDLMAEGKFDTDHFCVNTFVRKPLRSKYSRHNNKLIARFDHYCPWVYNDIGVRNHKLFMTFVYALNLAIVFFTWLSIEYFDQLKDAYESDDEDSSIVCALVGDDLCYGYKNHHFHYNLIMWCWLQYIWIVFLCLVQTFQILKGVTTWEFSNLNNKIQSSRFNHSTVPRDFGIDTAPAVQQQPPNGRETGYNTCMQLLGIDQFVLTLKLSTTSFFAKSGSSTANETLRTVDIPTDYGFKQNWLDFWIIGEPNWRNVFYLPIKGENNLNGEVVDYYKLYEYPAKSATQVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANG5</accession>
    <name>G3ANG5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">adenosylmethionine decarboxylase</fullName>
            <ecNumber evidence="4">4.1.1.50</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_55532</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic AdoMetDC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.50" evidence="4"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375231.1">
        <property type="nucleotide sequence ID" value="XM_007375169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANG5"/>
    <dbReference type="FunCoup" id="G3ANG5">
        <property type="interactions" value="595"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANG5"/>
    <dbReference type="GeneID" id="18871945"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55532"/>
    <dbReference type="eggNOG" id="KOG0788">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023050_0_1_1"/>
    <dbReference type="InParanoid" id="G3ANG5"/>
    <dbReference type="OMA" id="WFEESSN"/>
    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="UniPathway" id="UPA00331">
        <property type="reaction ID" value="UER00451"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004014">
        <property type="term" value="F:adenosylmethionine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006597">
        <property type="term" value="P:spermine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.90.10">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048283">
        <property type="entry name" value="AdoMetDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001985">
        <property type="entry name" value="S-AdoMet_decarboxylase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016067">
        <property type="entry name" value="S-AdoMet_deCO2ase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018166">
        <property type="entry name" value="S-AdoMet_deCO2ase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00535">
        <property type="entry name" value="SAM_DCase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11570">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11570:SF0">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE PROENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01536">
        <property type="entry name" value="SAM_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001355">
        <property type="entry name" value="S-AdenosylMet_decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56276">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01336">
        <property type="entry name" value="ADOMETDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 17" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="6" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="13" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="12" id="KW-0704">Schiff base</keyword>
    <keyword evidence="8" id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_5042322509" description="S-adenosylmethionine decarboxylase beta chain" evidence="18">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042322510" description="S-adenosylmethionine decarboxylase alpha chain" evidence="18">
        <location>
            <begin position="89"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="14">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for catalytic activity" evidence="14">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for processing activity" evidence="14">
        <location>
            <position position="308"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for processing activity" evidence="14">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autolysis" evidence="17">
        <location>
            <begin position="88"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="16">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW31955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="421" mass="48485" checksum="E2AB2CD1D280BAFA" modified="2011-11-16" version="1">MVAPAYFQDTYVDHELSANLDSTFAFEGPEKLLEIWFWPSETQLPKDIPADGIRSIPLESWVKLLDLVNCKILSMKSSKFMDAYLLSESSLFVFPHKLILKTCGTTTTLACLDELFETVREKMNLLLSSKEIYKVFYSRRSFMFPDKQLSVHKDWKSEVSLLDKHFAHGKSYIVGNFNSDDHWYLYVGGYEDHVNPHIQANDQTFEILMTELNPECAHKFVTSRKPGVESLIKDVVDEHDLGHDIGLETMVETSLDEVFEPTRNFTSHLPSPSLSGISECEEEIQVVKQPTKFEFIHDAFSFTPCGYSSNSICSNVPGGYYYTLHITPESGWSYASFETNYPFSDKSPVDIMTVITRILNIFEPRKFSMTLINDLLNDNSSLFMLKNSDLMLKDLGYKKQERVLYDLKNEYSLLYLKFEKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANJ9</accession>
    <name>G3ANJ9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alkyl transferase</fullName>
            <ecNumber evidence="3">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_138943</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="3"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375804.1">
        <property type="nucleotide sequence ID" value="XM_007375742.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANJ9"/>
    <dbReference type="FunCoup" id="G3ANJ9">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANJ9"/>
    <dbReference type="GeneID" id="18870162"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138943"/>
    <dbReference type="eggNOG" id="KOG1602">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038505_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANJ9"/>
    <dbReference type="OMA" id="TKGQPDP"/>
    <dbReference type="OrthoDB" id="4173905at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904423">
        <property type="term" value="C:dehydrodolichyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043048">
        <property type="term" value="P:dolichyl monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1180.10:FF:000005">
        <property type="entry name" value="Alkyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF2">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT SRT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="315" mass="36095" checksum="39496EB485F555C3" modified="2011-11-16" version="1">MASANFFPYSVNSLVRLIVQFPLFAYIVGFLEDFVIGIMRTGPIPKHIALIMDGNRTYAKNHRLPLKDGHFAGANALVKVLEVCYKVGIDQVTIYAFSLENFNRSKEEVDTLFGMLRDKLKLISENEESYARYNKVRIKVIGNRSFIPDDILKDLEYIEQITKEKSSRKTLHVCFPYTARDEITHSIKSIVQKRVLGEIESQDDITAATIEENFYFGKDVYPLDILVRTSGHTRLSDFMLWQCNEGCTIEFPDVLWPDFGFLCIVSVLFKWSYYKTIQLEEEAIQGKKPNLKEVIPPVLLHELPSPPPAASVSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANL7</accession>
    <name>G3ANL7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_51092</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375822.1">
        <property type="nucleotide sequence ID" value="XM_007375760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANL7"/>
    <dbReference type="GeneID" id="18871514"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51092"/>
    <dbReference type="HOGENOM" id="CLU_604332_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANL7"/>
    <dbReference type="OrthoDB" id="10513184at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="43"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="453" mass="53612" checksum="E1BEB04369A8D525" modified="2011-11-16" version="1">MQLTISINLDIQPMRNIAELVSVAVGPTPSPPSCEDRSAHPDPLSVLSLPDELLSIILAHINQLDTCSVLATHSRFREVGQRKLFRYIYVTSHDTQLVVSSNFYTPFYTNFTVIGASAFDCIRFRPLDLVKKIVFDQHYGYTDWFKNTMKVYPHINFFVELPHYKEQLEYLQNNQIGRLRRFVLDQSIIFQDYQNENRLFVEELNVLKIPRCNPDLKVLQYLPNLRVLRIRDMLDVVHHAFRFGNWKKLHIEELSIFWKQDLLPEYITDLKYMFNLSSLESLELHFCMDNEVSPSYESDLQQMLTRTTNLTQLSLTCQHINFAEAIGMLKPNTLTSFHVGIVWERRFRLLGYEFDIRKVLNRQQESVERVSINDLIEKCECSSQLLEWDRFYKNVKACSTNDRYMYYKNKDEGAIRKVLQSGYLKLRQVVFDRRYYHISYAPHMNLKDIVRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANW5</accession>
    <name>G3ANW5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61659</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375866.1">
        <property type="nucleotide sequence ID" value="XM_007375804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANW5"/>
    <dbReference type="STRING" id="619300.G3ANW5"/>
    <dbReference type="GeneID" id="18873993"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61659"/>
    <dbReference type="eggNOG" id="ENOG502S7CA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_543116_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANW5"/>
    <dbReference type="OMA" id="HHFGRTG"/>
    <dbReference type="OrthoDB" id="5600085at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="467"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="476"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="502" mass="54785" checksum="7DE1EA87C9BAF180" modified="2011-11-16" version="1">MSASERAKKKSLINAANADIKKKASETNFTPSDSPQSQQSQNLNQEQSQPETPSYSSYSNPNSNFVIDDIVIPFDVGNGLFDLFSHHSDGNLTDYNSQRSTPSQQILQNGSSIKHEQNNTPSTNNDFNDDTNSQYNLDAINQPTNSNTNIAKSNSIDTNSNNNNNNNNTDRSQDDFSPNKGITNHLSPTEIDLVDHMFPLFPLVGNQSFESENQPLSTIPQEIIKPSKPPPPQQPKPMRPTVMTHSSSLQNGMASSVNSINSVATSTSTTGNHNQFTSVRLKRPESVLSITSNSSSRSYDVVGNPTAYNQGTFISGSLPMSSTSAAFPPSDAFNNSNSNDDNFGHHSHHFGRTGLGSTSNIGTQLTKIESELYTESFFDENMDSVNINQPNLSASSSVPNFYGVQDSAPANGSVPDLTFANNNDSVSDFGYDKASSVPTNPLEYDIPLYQEMFTPIMGSKPYVFDQFNDYKDQSHQQKQSPITSQAKSSPLKQSPQGQQAQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANZ5</accession>
    <name>G3ANZ5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DNA-directed RNA polymerases I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_61683</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal Rpo12/eukaryotic RPC10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375896.1">
        <property type="nucleotide sequence ID" value="XM_007375834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANZ5"/>
    <dbReference type="FunCoup" id="G3ANZ5">
        <property type="interactions" value="601"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANZ5"/>
    <dbReference type="GeneID" id="18874004"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61683"/>
    <dbReference type="eggNOG" id="KOG3507">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179456_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANZ5"/>
    <dbReference type="OMA" id="MTYICGD"/>
    <dbReference type="OrthoDB" id="5585087at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.30:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.30">
        <property type="entry name" value="RNA polymerase ii, chain L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006591">
        <property type="entry name" value="RNAP_P/RPABC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039747">
        <property type="entry name" value="RPABC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12056">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12056:SF2">
        <property type="entry name" value="GEO11084P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03604">
        <property type="entry name" value="Zn_ribbon_RPAB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00659">
        <property type="entry name" value="RPOLCX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW32620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="73" mass="8052" checksum="3F4392A6CD520866" modified="2011-11-16" version="1">MASPQREGFSIPSSISHAALGQVTSKSLGVKYNCAQCAASFSLSKNDAIRCKECGHRVIYKSRTRRMVQFEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP03</accession>
    <name>G3AP03_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">5-demethoxyubiquinone hydroxylase, mitochondrial</fullName>
            <shortName evidence="8">DMQ hydroxylase</shortName>
            <ecNumber evidence="8">1.14.99.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Ubiquinone biosynthesis monooxygenase COQ7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">COQ7</name>
        <name evidence="9" type="ORF">SPAPADRAFT_61134</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the hydroxylation of 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) during ubiquinone biosynthesis. Has also a structural role in the COQ enzyme complex, stabilizing other COQ polypeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 5-methoxy-2-methyl-3-(all-trans-polyprenyl)benzene-1,4-diol + AH2 + O2 = a 3-demethylubiquinol + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:50908"/>
            <dbReference type="Rhea" id="RHEA-COMP:10859"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:84167"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="EC" id="1.14.99.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="8">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion inner membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the COQ7 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.60" evidence="8"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375310.1">
        <property type="nucleotide sequence ID" value="XM_007375248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP03"/>
    <dbReference type="FunCoup" id="G3AP03">
        <property type="interactions" value="383"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP03"/>
    <dbReference type="GeneID" id="18873733"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61134"/>
    <dbReference type="eggNOG" id="KOG4061">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071892_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP03"/>
    <dbReference type="OMA" id="WSTAVMG"/>
    <dbReference type="OrthoDB" id="275371at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008682">
        <property type="term" value="F:3-demethoxyubiquinol 3-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160224">
        <property type="term" value="F:3-demethoxyubiquinone 3-hydroxylase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01042">
        <property type="entry name" value="DMQH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01658">
        <property type="entry name" value="COQ7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011566">
        <property type="entry name" value="Ubq_synth_Coq7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11237:SF4">
        <property type="entry name" value="5-DEMETHOXYUBIQUINONE HYDROXYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11237">
        <property type="entry name" value="COENZYME Q10 BIOSYNTHESIS PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03232">
        <property type="entry name" value="COQ7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0830">Ubiquinone</keyword>
    <keyword evidence="2 8" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="250" mass="28301" checksum="0EC9FC605FB0FCE7" modified="2011-11-16" version="1">MISRSIPKRQALASIRQLSNTSRLLQVTEQQDQPQDKKYTRDELTYGKLSPAQKAYLDRVIRVDQAGELGANYIYLGQILVLASRYPHLKPLLTHMREQEIHHHNTFNDLQVRRRVRPSLITPLWKVGAIGMGMGTALLSKEAAMACTVAVETVIGGHYNEQLRVLMNQYNVPIYDKQGKVVDKSEVTPTAIVSEELTSLKENIKLFRDQELEHLDIALENDAEKAVPYKLLTEGIKTVCKGAIWAAERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP04</accession>
    <name>G3AP04_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kinetochore protein Nuf2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_50632</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NUF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375311.1">
        <property type="nucleotide sequence ID" value="XM_007375249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP04"/>
    <dbReference type="FunCoup" id="G3AP04">
        <property type="interactions" value="317"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP04"/>
    <dbReference type="GeneID" id="18871466"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50632"/>
    <dbReference type="eggNOG" id="KOG4438">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025461_2_0_1"/>
    <dbReference type="InParanoid" id="G3AP04"/>
    <dbReference type="OMA" id="YLKMEAH"/>
    <dbReference type="OrthoDB" id="8194677at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.60">
        <property type="entry name" value="Ncd80 complex, Nuf2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005549">
        <property type="entry name" value="Kinetochore_Nuf2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038275">
        <property type="entry name" value="Nuf2_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03800">
        <property type="entry name" value="Nuf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Nuf2 N-terminal" evidence="11">
        <location>
            <begin position="43"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="192"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="315"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="427"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="18"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="486" mass="56489" checksum="6E19605A95545114" modified="2011-11-16" version="1">MAPPRRMTPSQARRRSTIQPSSQISSQQSSQQARTEYESIGDDLFPLLPPAEIADLLAYCYFEVNEELIKKPTSSFVFSLYEQLLEQCLAIPIGRVETVARRIARDGDQQDEGEVDESEMVMNRYMVLTKFFGKFLKDSGFHDFGLFDVAKPDPLRTQRILSAVSNYLRFREHQSPIFEEIAHDVDLKMEELADVQEEHNAALNVKQELEDKYGKTPEERQEKLRAQNAYNALIERDVEQLQQTLQVLEQEYNEYERNKLDLKQKFEDLHGLVEVERIKIKSLQEVKSTDIKVLQDVMNNSKSRAAGRQQEIERLKQVKHNYETTIRSIETVEQELHVLLTLSRKLKESNDDIDSIKTTIRDVQNAINDLNPIKERLEEEVKNLEHQFVDTNTKLADLNKLVEVRSKQADDSFNASKIEYERGIAEIEKTLLACEQLKISYAELENDTSKIIREYEDEEQEAKEKLAQLSDKLQQYMQVANPILQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP69</accession>
    <name>G3AP69_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_61700</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="2"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375916.1">
        <property type="nucleotide sequence ID" value="XM_007375854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP69"/>
    <dbReference type="FunCoup" id="G3AP69">
        <property type="interactions" value="616"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP69"/>
    <dbReference type="GeneID" id="18874011"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61700"/>
    <dbReference type="eggNOG" id="KOG1815">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009823_4_1_1"/>
    <dbReference type="InParanoid" id="G3AP69"/>
    <dbReference type="OMA" id="PYAYYMD"/>
    <dbReference type="OrthoDB" id="10009520at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000002">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045840">
        <property type="entry name" value="Ariadne"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19422">
        <property type="entry name" value="Ariadne"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="143"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="147"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="557" mass="64385" checksum="B672718362DC40E1" modified="2011-11-16" version="1">MSDDDSDIFFEYEYDQEDDLDESSFCFSSDGEDDVMFDVDGENSDTPQTKISDTLANVGANGTLYYPWNVEQFIENKLLAKLDKLANGTLTACSIDELLIMLQVKGWQEDEVLNDYFDNRDKLYEACGLPVGVPMRNTLKKIKNYSCFVCCEDYSETYVYSLTCGHTYCINCYYSYISNELANGGPITCIEPDCKYTIPYRDVTDIFDIVNKTNHGGVTTIKSMVENPLLVANTKAMINSKRKYKWCPATDCNGFAELVGNVNDSVESLSSAKESVDISKVPIVTCSENHEFCFDCNYENHLPCPCWIVKKWIKKCNDDSETANWIDANTHGCPQCQSAIEKNGGCNHMTCKKCKFEFCWICFEDWKKHRNDYYSCNKYRNERQEDEQRKNRSKQSLERYLHFYKRFAIHENSMKGDLKTMAQIDTYTRLYMEDLRDQGKKNLSWNDIQFLPTAMRALQNGRKALKWTYCFAYYLGKSNFATIFEGNQDFLNKTVEDLSEVFEQIMSKKNPDKVGMILKNKTKLRNLSELVNSRKNMLINGARANLDDGLLWFDNEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP78</accession>
    <name>G3AP78_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative proline permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_51185</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375925.1">
        <property type="nucleotide sequence ID" value="XM_007375863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP78"/>
    <dbReference type="FunCoup" id="G3AP78">
        <property type="interactions" value="153"/>
    </dbReference>
    <dbReference type="GeneID" id="18871530"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51185"/>
    <dbReference type="eggNOG" id="KOG1286">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_12_1_1"/>
    <dbReference type="InParanoid" id="G3AP78"/>
    <dbReference type="OMA" id="SMFLLIW"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000006">
        <property type="entry name" value="General amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF36">
        <property type="entry name" value="PROLINE-SPECIFIC PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="423"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="465"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="494"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="63"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="567" mass="62935" checksum="F42891A07101A3B1" modified="2011-11-16" version="1">MASPHHMSIVRGNDVNLSISISNSRVVDVDEKAGNIHEIISSGSGSDLEYQEKTLERGLKSRHVQLIAIGGAIGTGLFVGSSVVLTSCGPASLFLAYCIMSTVIYFVMQMLAEMTTFLPLPGNGPQAFVNDYLSHSFGFAIGYNYWYAFSILVAAEITAAALVIQYWTTKVNIAVWITILLALMISLNLISVKFFGESEFWFASIKLFALMGLIILGVVLFFGGGPNHDRLGFRYWKHDAFKEHLVKGSTGRFLGFWTALIKSGFAFICSPELVSTAGGECYKPRRNLPKAARRFIYRLAFFYVLGTLIIGVIVSSNDKKLINAGTNDASGSPFVIGIQNAGIPVLNHIINAVILTSATSAGNSFLYSSSRALFSIAQRGSAPRIFTTVNRFGVPYYAVAASSSFGFLSYLNCSSAAATVFTWFSNISTMSGFISWLTVTWAYLRWRKAIDHHNLHDRVTFRTPLQPYGAYYVLFVISLITITNGYAVFLDFNIGDFLAAYITFPIILVLYFGHRIYEYKVHNIKNWFIPIEKIDLVTGLDLVEEEDATSPEPIPRNFIEKIWYWIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3APE1</accession>
    <name>G3APE1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_61203</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the peptidase M18 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375394.1">
        <property type="nucleotide sequence ID" value="XM_007375332.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APE1"/>
    <dbReference type="STRING" id="619300.G3APE1"/>
    <dbReference type="GeneID" id="18873774"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61203"/>
    <dbReference type="eggNOG" id="KOG2596">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019532_3_1_1"/>
    <dbReference type="InParanoid" id="G3APE1"/>
    <dbReference type="OMA" id="ANHEEIG"/>
    <dbReference type="OrthoDB" id="9880441at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.250.10">
        <property type="entry name" value="Aminopeptidase i, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001948">
        <property type="entry name" value="Peptidase_M18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023358">
        <property type="entry name" value="Peptidase_M18_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570">
        <property type="entry name" value="ASPARTYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570:SF4">
        <property type="entry name" value="VACUOLAR AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02127">
        <property type="entry name" value="Peptidase_M18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00932">
        <property type="entry name" value="AMINO1PTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101821">
        <property type="entry name" value="Aminopeptidase/glucanase lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="8"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="507" mass="56005" checksum="762FC8242D1FD0BA" modified="2011-11-16" version="1">MHSRKHSTTSISSIETPSESEFASSPLLPPPAPPAPHDRYQDYYDAYASHFMEFLNESPTTYHVITHFKSLLENNGFTFIPQTETVDDLPPGYYFTSRDDQCLVAFIVGGKWKPENGSCFVGCHVDALTVKLNPRGSLSGTTEGYETLGVAPYSGSLNKLWLNRDLGLAGSILVRQSDGKIVRKLISSFPHPIGTIPSLAPHFGEVDQYNKQTKMVPVMGYLSEEEEKEPASEEEKNSKFYGRHSLSLLRYICKLADVPISSVVDIDLQLDDVQPAQRGGLSNEFIFSPSLDDRLCSYSAIYGLIEFSQKFYQKLDISTYEGLAGVYLANNEEIGSATRTGAGGGLLIDTFKSIVSSRSHTVTESLAQLTTNTVIISSDVTHALNPNFQSVYLKNHAPFPNTGPCIKLDSNFHVLSDSPSYVFLQQIMTHLPGIKLQQFHIRNDSRSGGTIGPIMSNGCRGINGARIIVDVGLPILSMHSIRGMCGYKDVGMGCRFFKAVFEFWQDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3APY4</accession>
    <name>G3APY4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">4-hydroxy-4-methyl-2-oxoglutarate aldolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_50872</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375581.1">
        <property type="nucleotide sequence ID" value="XM_007375519.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APY4"/>
    <dbReference type="FunCoup" id="G3APY4">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APY4"/>
    <dbReference type="GeneID" id="18871494"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50872"/>
    <dbReference type="eggNOG" id="ENOG502RXXY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072626_0_1_1"/>
    <dbReference type="InParanoid" id="G3APY4"/>
    <dbReference type="OMA" id="MTAYEDF"/>
    <dbReference type="OrthoDB" id="1476984at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047443">
        <property type="term" value="F:4-hydroxy-4-methyl-2-oxoglutarate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008948">
        <property type="term" value="F:oxaloacetate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16841">
        <property type="entry name" value="RraA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.40">
        <property type="entry name" value="Ribonuclease E inhibitor RraA/RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005493">
        <property type="entry name" value="RraA/RraA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036704">
        <property type="entry name" value="RraA/RraA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33254:SF28">
        <property type="entry name" value="4-HYDROXY-4-METHYL-2-OXOGLUTARATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33254">
        <property type="entry name" value="4-HYDROXY-4-METHYL-2-OXOGLUTARATE ALDOLASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03737">
        <property type="entry name" value="RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89562">
        <property type="entry name" value="RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="109"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605493-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="26159" checksum="73941EA9E1744374" modified="2011-11-16" version="1">MKIPTSSKEIIIFLKQFTPCDVSDSLNKHGVASGGFIPNLINFSPTAPGESAVGKAYTVLYAPKSDPRPAVKNAYIDSVPPDSIVVIGLPLDLQTTTAPYTKVNNAMYGGLMSTRAQYSKANGSVIFGRIRDLDEHNDLKYPVWSYGVGTSAPGSLLKVVGINIPLQVKVHGLSQDEEVITVNPGDYIIGDKNGVVSLPDDEQLPSVLEYIPKRVEADTKVSQDIKNGKPAAESQKFWRSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ23</accession>
    <name>G3AQ23_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glucoamylase 1</fullName>
            <ecNumber evidence="2">3.2.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">1,4-alpha-D-glucan glucohydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Glucan 1,4-alpha-glucosidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_139122</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.3" evidence="2"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375620.1">
        <property type="nucleotide sequence ID" value="XM_007375558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ23"/>
    <dbReference type="STRING" id="619300.G3AQ23"/>
    <dbReference type="GeneID" id="18870180"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139122"/>
    <dbReference type="eggNOG" id="KOG1065">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000631_11_0_1"/>
    <dbReference type="InParanoid" id="G3AQ23"/>
    <dbReference type="OMA" id="PYVINHD"/>
    <dbReference type="OrthoDB" id="1334205at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004339">
        <property type="term" value="F:glucan 1,4-alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06602">
        <property type="entry name" value="GH31_MGAM_SI_GAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000001">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000138">
        <property type="entry name" value="Putative alpha-glucosidase AgdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030458">
        <property type="entry name" value="Glyco_hydro_31_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030459">
        <property type="entry name" value="Glyco_hydro_31_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF133">
        <property type="entry name" value="P-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00129">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00707">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442539" description="Glucoamylase 1" evidence="14">
        <location>
            <begin position="22"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="15">
        <location>
            <begin position="301"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="16">
        <location>
            <begin position="735"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="525"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="525"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW32344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="950" mass="105385" checksum="CB4A04CF52408B1F" modified="2011-11-16" version="1">MILSSVVKTAIALAMLGYSAASPTPSSSSVADSHPTVPNELTLGVKQVPNILNETAVDANQVAKGYSLTNVTATPRGLTGLLNLKGATNIYGYDFDKLNLTVTYQSDNRLNVRIAPVNLTDVYILPESLVALPKVEGDVNTFEFENSDLIFSYDSENFGFEVKRASTAEVLFSTKGNPLVFSNQFVQFNTTLPKGHQITGLGESIHGSLNEPGTVKTLFANDVGDPIDGNIYGVHPVYYDQRYDTGTTHGVYWRTSAIQEVVVEDQSLTWRALSGVIDLYFFSGPDPKDVIQQYVKEIGLPAMQPYWALGYHQCRWGYDTIDDVKNVVENFKKFNIPLETIWTDIDYMDTYKDFTNDPYRYPTDKYQQLLEELHSNNQHYVPIFDAAIYVPNPNNATDNAYTPFHAGNESDIFLKNPDGSLYIGAVWPGYTAFPDFLNNKTQEYWNEQFKTWHDRIPFDGIWTDMNEVSSFCVGSCGSGRYFDNPVHPPFAVGAPATSYPLNFNVTNATEWASISSAIAATASTPVPEATSSSSSSIDSKNTLAPGKGNINYPPYAINNAQGDHDLATHAVSPNATHVDGTVEYDIHNLYGFTQEKAIYNALLNIAPDKRPFIIGRSTFAGAGHYVGHWGGDNSADYDMMYFSIPQAFSMGLSGVPFFGVDVCGFNGNTDMELCSRWMQLGSFFPFYRNHNVLGAISQEPYVWESVTEAAKTSMNIRYLLLPYYYTLLHESHTSGLPILRSFNWEFPYDKKLSGIDNQFFVGDALVVTPVLQPGVDKVKGVFPGTDEVYYDFYTTEKQNFKNDKNETLDAPLGHIPLHIRGGHILPTQEPGYTTTESRKNPFGLLVALDKDGKAAGKLYLDDGESLNVTESLYVDFVAGDNELSVSAFGDYKVEQPLANVTILGVDKKPEGVKFGDSKVNYKYEKGTVYITGLEKYTKEGAFAEEFTVSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ39</accession>
    <name>G3AQ39_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dynein heavy chain, cytoplasmic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Dynein heavy chain, cytosolic</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_51404</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Consists of at least two heavy chains and a number of intermediate and light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376164.1">
        <property type="nucleotide sequence ID" value="XM_007376102.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AQ39">
        <property type="interactions" value="742"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ39"/>
    <dbReference type="GeneID" id="18871551"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51404"/>
    <dbReference type="eggNOG" id="KOG3595">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000038_7_0_1"/>
    <dbReference type="InParanoid" id="G3AQ39"/>
    <dbReference type="OMA" id="NERQMTR"/>
    <dbReference type="OrthoDB" id="447173at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072384">
        <property type="term" value="P:organelle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000002">
        <property type="entry name" value="Cytoplasmic dynein 1 heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2620:FF:000001">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.100:FF:000002">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000002">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000071">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002357">
        <property type="entry name" value="Glutathione S-transferase class-mu 26 kDa isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054354">
        <property type="entry name" value="DYNC2H1-like_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013594">
        <property type="entry name" value="Dynein_heavy_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703:SF36">
        <property type="entry name" value="DYNEIN HEAVY CHAIN, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08385">
        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22597">
        <property type="entry name" value="DYN_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0243">Dynein</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="1853"/>
            <end position="1990"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="2141"/>
            <end position="2310"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="2497"/>
            <end position="2647"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="2839"/>
            <end position="3004"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="808"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="3109"/>
            <end position="3136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="3332"/>
            <end position="3401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW31386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="4210" mass="482370" checksum="E664493384672AF1" modified="2011-11-16" version="1">[encoded data, 4210 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ75</accession>
    <name>G3AQ75_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Maintenance of telomere capping protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_61987</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in telomere capping.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the MTC6 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376200.1">
        <property type="nucleotide sequence ID" value="XM_007376138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ75"/>
    <dbReference type="FunCoup" id="G3AQ75">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ75"/>
    <dbReference type="GeneID" id="18874136"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61987"/>
    <dbReference type="eggNOG" id="ENOG502QVFP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033723_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQ75"/>
    <dbReference type="OMA" id="WGTIDPQ"/>
    <dbReference type="OrthoDB" id="5573651at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051008">
        <property type="entry name" value="Telomere_Capping_Maintenance"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057530">
        <property type="entry name" value="TIM-barrel_MTC6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35518:SF2">
        <property type="entry name" value="MAINTENANCE OF TELOMERE CAPPING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35518">
        <property type="entry name" value="MAINTENANCE OF TELOMOERE CAPPING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25506">
        <property type="entry name" value="TIM-barrel_MTC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442495" description="Maintenance of telomere capping protein 6" evidence="11">
        <location>
            <begin position="18"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="502"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="MTC6 partial TIM-barrel" evidence="12">
        <location>
            <begin position="22"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="553" mass="63316" checksum="6A3BFE071D392024" modified="2011-11-16" version="1">MIKVIVTWLWFIHIILCLENWPTLSDTLQVAIRSQRDVSMTVPIDQISNAGGSLNSMIFDQYGYTMDSMSYVDNFLTVGVQTFMVDLYWNEFTQVWQLCPAPIPNNVTSNIVDINWNNRSYKCDTNISTQTFLNRFNNFILETNTNFEVSLITVLLNLKSIHYTKSNMTIGYDSLFNHTIPGNSTLNATFSGLGTSLFTPEVLQTYRNTVAVDTTNMGFYNSSSALMPSLNMVLYTELRRIMLNVVSNELISSTRGYTITENDKMGLFFNDTLPTSIKATNSESMTHLCQNLVENFDTERTNFRFIIDNDFDPFTLSTVRQYIRCGLTPIFNATAYKVNSTRIRDLGQIFNAFIPYSLWSWAPFQPVTPNNQFTNSSSMSNGTIPYRCVLLNTNGWEVANCYDSYPFACQNSSSPHDWYIDPNKQKSYFDIDHDDCPEDFKFSLPRSNGEMLSLMNMTDTEVLPIWIDLNDLTVDNCFVSGGPYAECPYSKTITANTFVKSIAPSVVVGVVILILIFFEKVFRSNPIQTNRKRYWKRTLQDYYEKNDYEGVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQH6</accession>
    <name>G3AQH6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_141177</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376301.1">
        <property type="nucleotide sequence ID" value="XM_007376239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQH6"/>
    <dbReference type="STRING" id="619300.G3AQH6"/>
    <dbReference type="GeneID" id="18870351"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141177"/>
    <dbReference type="eggNOG" id="ENOG502RY54">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053891_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQH6"/>
    <dbReference type="OMA" id="CSKNEWT"/>
    <dbReference type="OrthoDB" id="4073795at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="20"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="372" mass="44473" checksum="42554341C8CD6811" modified="2011-11-16" version="1">MKRKQEEEGLSHKFRKTNHELSLLNIPDDVLNLILSHLSLTDVIHLSCLDRAYRSCFSSRIFTQVKAPWSKLIEAMDSKENFIIKHKNIIRQLRIIDSYSYGEWQIDFFTDLLINIPNLKRLLINSKNSSNWIKYRGSDNIEQLSLYFEEDSFNLIKFSLPNAKQGNFNSSPRIFRLDHVANFKMLTKLSLTDYHFNWEVESVKPVLMIHDISLHNCSWEYPFKLSQFNYNDNIKKLKLTYSNQNPFLLSERFNTFLNDDYHEDFKSLEELAIDITFDFRKQAWSKFLSNRLILRFYNRKDFPNLRQLTLHGWYFALQNFKETIKNISCSDSDLYLLDLKVFDSVNDCNLIPEIKQEWKQLCVWKKLKLEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQK1</accession>
    <name>G3AQK1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">OPA3-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_72332</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OPA3 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376326.1">
        <property type="nucleotide sequence ID" value="XM_007376264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQK1"/>
    <dbReference type="STRING" id="619300.G3AQK1"/>
    <dbReference type="GeneID" id="18875426"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72332"/>
    <dbReference type="eggNOG" id="KOG3335">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074707_3_2_1"/>
    <dbReference type="InParanoid" id="G3AQK1"/>
    <dbReference type="OMA" id="WAEFEGT"/>
    <dbReference type="OrthoDB" id="2129069at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019216">
        <property type="term" value="P:regulation of lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010754">
        <property type="entry name" value="OPA3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12499:SF0">
        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12499">
        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN OPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07047">
        <property type="entry name" value="OPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="17344" checksum="52647DBE63A788FA" modified="2011-11-16" version="1">MSSIALKLTSLLVKTVAKPMANAIKRQAKEHETFRNICIRMAQTLHSGELRLRMSLLGEKKIRVRPLNDTKAIEQGANFISEFFIFSVAGSLIFYESYRSRKKASEARESLADDISVLQSEIEYIKTKLGDINIKLDDYVVPDGTIIPGNIPRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQL7</accession>
    <name>G3AQL7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_141554</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376342.1">
        <property type="nucleotide sequence ID" value="XM_007376280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQL7"/>
    <dbReference type="GeneID" id="18870382"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141554"/>
    <dbReference type="eggNOG" id="KOG0255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008455_1_3_1"/>
    <dbReference type="InParanoid" id="G3AQL7"/>
    <dbReference type="OMA" id="QNTAQMM"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF60">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="384"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="431"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="497"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="529"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="565"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="154"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="602" mass="67897" checksum="81BCF23F05B8BBB1" modified="2011-11-16" version="1">MNKSKESLEIYNRELESSSSNDSNEVLAFTVSNYEQQQQQGQGQGRVRKSLDSIFRPRSNSVSSNIDQFSVHHIYGALPDEKIEIKRTQTRATVLSEIATNNFPHDDLKIQEEKIIIPDDGKEFTEIDPELITWEDEQDPANPRNWDLRTKVIMLMFVSFYAFVAPMSSSILSPAMSFISEEFNIESGSPLQAMVVSIQILAWAIGPLIIAPLSEHDNIGRKLVLDGSCLMSLLFNLGCAFSRNTAEMMVFRFLAGIFGCVPMNVCAGVISDMFDANGRNVALASYSLVPLLGPVIAPLVAGFIVVNLQWRWVFYVLCIFNGTVFITALIFFKETYAPTLLKRKADKLRKETGNDNLHTIYEMMEQEAGESTLQKMIHCMIRPITLLFMHPMIVGLGSFMAFTYGFMYLMIVTFPEIFGEQYGFKKNIIGLMYLPMGFGFVLGVVFWTFCIGKVYKYLVVRNNGEGKPEFRLPCLIFCSLFIPVGLIWFGWSAQKKLHWIMPGIGSALFSFGLVCVFQTTQNYLIDMNVRYAASSVAAAALFRSLFGFAFPLFANKMYGKLGYGWANTMCAFIGIALGVPFPIFCYLYGERIRNWADKRIGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQM4</accession>
    <name>G3AQM4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome b-c1 complex subunit 6, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Complex III subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_62185</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UQCRH/QCR6 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376349.1">
        <property type="nucleotide sequence ID" value="XM_007376287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQM4"/>
    <dbReference type="FunCoup" id="G3AQM4">
        <property type="interactions" value="115"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQM4"/>
    <dbReference type="GeneID" id="18874207"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62185"/>
    <dbReference type="eggNOG" id="KOG4763">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115913_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQM4"/>
    <dbReference type="OMA" id="NTCNDRV"/>
    <dbReference type="OrthoDB" id="405848at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.20:FF:000003">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.20">
        <property type="entry name" value="Ubiquinol-cytochrome C reductase hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003422">
        <property type="entry name" value="Cyt_b-c1_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023184">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036811">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15336:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15336">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 7.8 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02320">
        <property type="entry name" value="UCR_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81531">
        <property type="entry name" value="Non-heme 11 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ubiquinol-cytochrome C reductase hinge" evidence="12">
        <location>
            <begin position="43"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="10"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="19"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="114" mass="13590" checksum="532B6C08ADEC6626" modified="2011-11-16" version="1">MSFLRDLIEAVVPSAHAEEPEEEVEESVEAEEEEEEDEEELVDPLDTLREECEKTEVCKPFAHHFHECVERVTKEQEDEDYHKKDYKEDCVEEFFHLQHCVNDCVAPRLFHKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQN1</accession>
    <name>G3AQN1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Choline kinase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_62195</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the choline/ethanolamine kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376356.1">
        <property type="nucleotide sequence ID" value="XM_007376294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQN1"/>
    <dbReference type="FunCoup" id="G3AQN1">
        <property type="interactions" value="374"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQN1"/>
    <dbReference type="GeneID" id="18874213"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62195"/>
    <dbReference type="eggNOG" id="KOG2686">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012712_4_2_1"/>
    <dbReference type="InParanoid" id="G3AQN1"/>
    <dbReference type="OMA" id="CEQVINW"/>
    <dbReference type="OrthoDB" id="10267235at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004103">
        <property type="term" value="F:choline kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004305">
        <property type="term" value="F:ethanolamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05157">
        <property type="entry name" value="ETNK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007521">
        <property type="entry name" value="Choline_kin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603">
        <property type="entry name" value="CHOLINE/ETHANOALAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603:SF93">
        <property type="entry name" value="RE24176P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04428">
        <property type="entry name" value="Choline_kin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01633">
        <property type="entry name" value="Choline_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Choline kinase N-terminal" evidence="3">
        <location>
            <begin position="56"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="482"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="484"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="501" mass="58023" checksum="E1300C60A1AED717" modified="2011-11-16" version="1" fragment="single">MDITPSQSRSRSKSRTRNATRSRSNSTNRRTLHPRSLSSSSLTKLVITNTEIEGGERSIPSVEANLDNSLPLDFFKQDILAIIKTLKIHKWHKRQLSIANLTVTRISGALTNSIYKLEYKDDTHNLPSLLLRVYGKNVDELIDRNRELETLVKLSQKRIGPRLLGIFTNGRFEQFLDGFNTLNKESIRDEVISQMLGRRMKDLHYKIELDDKDLGPMPACWNLIEKWLKIFENDYLPGYAAANKDVQDIFWVDFQSFKQIVSKYKSWLFEKYEQTDFSSNYKFCHNDTQYGNLLLHESFNKDDIIMSTPLSSAATSLSSFEETAAKSTSNKKDSSLVVIDFEYSAANFPAFDIVNHFSEWMSDYYHEEKPYYIFNENYPTRAEQINLIRSYVEYDFQFPSSSLKTSKTIEQLINEPSSNLLQFEVEKMYNECILWRASVQIYWCLWGLIQNGPIKPVTTGKSEEKGIDSTYSITTDMENLTVEEASEEESIASSDDEFDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQN8</accession>
    <name>G3AQN8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SEC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_72363</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasmic vesicle</location>
            <location evidence="5">COPI-coated vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376363.1">
        <property type="nucleotide sequence ID" value="XM_007376301.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AQN8">
        <property type="interactions" value="1100"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQN8"/>
    <dbReference type="GeneID" id="18875429"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72363"/>
    <dbReference type="eggNOG" id="KOG0929">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000691_1_1_1"/>
    <dbReference type="InParanoid" id="G3AQN8"/>
    <dbReference type="OMA" id="EVMCAYI"/>
    <dbReference type="OrthoDB" id="18431at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030663">
        <property type="term" value="C:COPI-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140455">
        <property type="term" value="P:cytoplasm protein quality control"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000003">
        <property type="entry name" value="Brefeldin A-inhibited guanine nucleotide-exchange protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.20:FF:000002">
        <property type="entry name" value="Brefeldin A-inhibited guanine nucleotide-exchange protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015403">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046455">
        <property type="entry name" value="Sec7/BIG1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF375">
        <property type="entry name" value="LD29171P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20252">
        <property type="entry name" value="BIG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09324">
        <property type="entry name" value="Sec7-like_HDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SEC7" evidence="7">
        <location>
            <begin position="728"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="695"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="696"/>
            <end position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1850" mass="210707" checksum="00972BEA917476AB" modified="2011-11-16" version="1">MSESNNGDIENRDDSVVDSVEAPPAPSDPAPQGTETEDLQNTKHIDEDHVQENQEVTESEVVAEPVNGTTHQEEQQQEDTSEGPKEEADTEYTEDTEQQLEEQPLEDVQVKEKELPEVAEPTHEVSTETSPTESAPGSSLPEEPSTHHHQRDISHLSVSSTGATVDNTKLFQKTFELILTSKEAKKNEQFKTVIQKALDSLNDVDSRDPHVLFTALKACCETSSNDLKSKAVDLFAKLFDYAQFEDNDDKITMTDDSVDVISACFDGEGTDPELELQVVRALMHSILLMPCHGASLLKAVRQIYNVFIFSLTARNQAVAQGILTQVIGAIFQRVEESDAMKNNKNGKAASGTRLSLTSFSKEELELPSDDINPSEEKLTLKRLENLTDNGNDNDRVQEANLATESDEDVVVKDAFLVFRAMCKLSVKSLDSTTIDMKSHSVRSKLLSLHIIHTILREHIEVFLSHDVVILSSNSNEQIRLINAVRQYINLALSKNAASPLAPVFELSLEIFWLIISNLRSEFKREIPVFWDEIYFPVAEMKTSSPHQKRYLLSIMERLCNDSRCIIEFYLNYDCDSSMPNICEKIIDYLTRLALIRIDVTPQQKLAFRENRRNGISVYDVSKIANLTSSTMSSKPPEPEIYNQFPLEYALKMTSIGCSVAFLRSLYSWAQKGLNITPVAKSPAITTNGSALSLSKTVSESNNTSMSNSRNTSFINPVENSNETDDPEQFENQKQRKKAFLEGIRQFNQKAKKGMKYFVANGFLKSEEPEEMAKFLLETDGLDKSVIGEYLGEGDPQNIAIMHSFVDQMEFTNTDFVDAMRLFLQSFRLPGEAQKIDRFMLKFAERYVLGNPDVFSNADAAYVLAYSVIMLNTDQHSPQVKVRMTFENFVINNSGIDDGKDLPRDFLHKIYEEIQNNEIKLQSEQHAALLAGDVTLTPAPQSISFFGSRDVNREAYIHASKEMTTKTERLVRNLGKKLKNEESDGVFYAATSVLHVKSIFDTMWMSVLAALTPPFKEYDEEDITRTCLEGIKLSIRIACMFGLEYARTSFIGALVQFQNLNNYEEMKQKNVDAIYIMLDLAVCEGDHLGDAWLQILLSTSQLERLQLIAQGVDQDSIPDVSTAKLVNRNSVENPRTSTSFFSSFTYSSQTPSQSAASKFYNQHLSPEVAQLLTKTELEVAIDKVYTNSANLSGESIVDFVRALSEVANEEIESSGQSSNPRTFSLQKVVDICYYNMNRIRLEWSHLWAIIGETFNTVGCHTNPAISFFALDSLRQLSMRFLEIEELTHFKFQKEFLKPFEYVIIHNDSLEVKDMVLECINNMMMARAGQIKSGWKAIFGVCTAAAKENKESLVRKGFEIANRINKEYIEEVKQQDSFSDLVICFTEIAKNEKFQKLSLLSLDVLSRLIHEIAQLSFFSENEPHEDDTAEAKHERNEHLVKLWFPVLFAFYDIIMTGEELEVRSRGLNCLFNILLEYGKYFELEFWDMICHELLFPIFGVLNKHWELELDDSDDMLSVWLSTTLIQALKSMISLFSHYFDALSRLLDGYMKLIISCICQENDTIARIGRECLTTLLVDNCDKFDVEHWKQITEAFSTLFDLTTARELFTSDPLRNKRYDEEDAQKDIASKVDSEDTTNSHFDDEERLAKSREKSSIVVKSVLQLLLIQTLSELFEHDGFYESIPYEYLMKMAQLLHGSYNFAKKFNDDYDLRVRLWNAGIIERLPNLLKQESSSAAVFINIMFRMYCDDDKVSPGNKREIMDYIIPLCNTITERYSEFDETNQQRNISTWKPVIVEIFQGYVELDDEDFTKYTPVMYKLTLRLFSKSMSADLRSAIRTFLARVGEEFIKVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQS8</accession>
    <name>G3AQS8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nuclear pore complex protein Nup85</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_67664</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Functions as a component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the nucleoporin Nup85 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376403.1">
        <property type="nucleotide sequence ID" value="XM_007376341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQS8"/>
    <dbReference type="FunCoup" id="G3AQS8">
        <property type="interactions" value="147"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQS8"/>
    <dbReference type="GeneID" id="18875175"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67664"/>
    <dbReference type="eggNOG" id="KOG2271">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019986_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQS8"/>
    <dbReference type="OMA" id="VKHYSWM"/>
    <dbReference type="OrthoDB" id="17644at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011502">
        <property type="entry name" value="Nucleoporin_Nup85"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373">
        <property type="entry name" value="FROUNT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373:SF21">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07575">
        <property type="entry name" value="Nucleopor_Nup85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7 9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW31625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="740" mass="85704" checksum="6EED8D70DCFFEDA2" modified="2011-11-16" version="1">MGELGLTNFQDIEMLEIPEDEDISVSSDTEGISGSNTPDESNLTTMFKSNKEYIELSQWLKTDEELQFQFDSNQYDHSLKPTSEFEFAFIEYTKSLYKLVDKLTKDVAEQEKEEEEEEPIGVITAERSIYKLVNSPGLKIDEAFQEIVEQLKQLIQVTGESRSEEASKLQHFLFILQCLGANYFYFDIKRKPELVLEWVNTFHPKPDLELTETIMISNPEPYSHPQFWNVYMAQLITRGLFTQATQAIEHSQYDQLEESCPELYSIISDLDTLLADYSSFALKGQFPQWKLLACEFRDSLANVKIDDAKYGIILTQIYDLASIISGLPKTISQYCDTWYEVYLALALYKVRDDDDLFPEYFQQAINEKVPPQYTNSSLEDISDGCYLDILERNFLKMLKTMHELEPTTTAYIAQLLELKGLLQGYYNIPQTIRLTNKTISEYFLVRHAYESLNIHDLVPVGLGILLNNSICRSSYSHNRDVIGDFLPEYQCRTNDDLEWALTICAQLNLITAAKRLYYNYGLKSLDEGYIYESLNNFVHCYDSTGGEIHQEAMEKVHFIIWDLIFLDSITNNRPFQDELINNLVTKSVATTVDMHPVIRQCLAPYAVLKAFFDSLEDDSIKFSVKLSKLLHLLRFNYMPKKFMPLLLSQFLPFLTRPSSEFLLPDLVVMVEVIDNFENETNEEELKDAADLYVYCINHFEEESTSYDWRRIVKDVPSDVSSLTKTLRNQIAARIAKVYVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR90</accession>
    <name>G3AR90_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_154160</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376043.1">
        <property type="nucleotide sequence ID" value="XM_007375981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR90"/>
    <dbReference type="GeneID" id="18870971"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_154160"/>
    <dbReference type="eggNOG" id="ENOG502SF6T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029128_0_0_1"/>
    <dbReference type="InParanoid" id="G3AR90"/>
    <dbReference type="OrthoDB" id="4073735at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454:SF56">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="468" mass="54717" checksum="F667790C6DB73105" modified="2011-11-16" version="1">MLLRTNLRRLPDCVITQIFDSVPFDHLMEFCKHYPDKYVRDLLIQYYYCSRVQFAFNPPVPPPKATPGRRNLVTFVDSAVSQFMEDFPEVIPKTLLIDVCMDFKEAREILDKFHDRLVKCEVIDFALLEAEISADDFQFLLSFPNIKRVYFHFNSKLLLPALIDNPKLIKHPTLEHIIFCEHEITDWSGVRFPQNLKTLNLSYHRINFSTLILPQSVHSLSFIYCQIYSIKALRRQMTNELTELKITDNMLEHIVIDDLPRKLHTMDLRRNSIFSITGKEWPKGLKSLILSHNALDDDSLKVINNGVGWPEQLITLDLSVNFFDHTENLSNLPQSLKRLEISSNTFLWRDQENRVEFPVNLTYLGMSDCRVHNLSLLKFPVSLKELQLAHNGIKDLLPYGDWTELINLRQLDLFDNSITTLDNWIPPPNLRELIVENNPITRSPQFNELRNTFKFKITGMKSESNQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR97</accession>
    <name>G3AR97_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_67348</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376050.1">
        <property type="nucleotide sequence ID" value="XM_007375988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR97"/>
    <dbReference type="GeneID" id="18875157"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67348"/>
    <dbReference type="eggNOG" id="ENOG502SFR5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068277_0_0_1"/>
    <dbReference type="InParanoid" id="G3AR97"/>
    <dbReference type="OrthoDB" id="3984304at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="366" mass="41572" checksum="A2D81B29B7D46B1A" modified="2011-11-16" version="1">MLQRPVVEELTQPCSKPQPPPEPRQSQENIEPEIRTITIPSTSPIQRQENQLNQDHTIPVSTDNVHCHTAPVFKPAQFPLLGRIREPINNPIQINQVSTNNGTSNISPGFTNSSSSFFDIETSASQETQIPYSDIELSILSPHQPPYEPINHPSGSSDPAMRARLDRLEQRRSMFPHVDMFVEYTEERYRRFRPSDSQHNNEAIITGNHPYQSDKYESGTKPESSFFYRFTSWRKKPEQQQHQTPDLENQISPVLEGSSTQPHVKHLNLKPNTSRSIIIPSPSTITTEPGLTSAPVYTPKAIPINEYPLNFEKLPGDDDLRRTQIINSLDDEVRDLIDGVDNFIVECFAGIWSVMTAIGDFFVTLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARA3</accession>
    <name>G3ARA3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">mitogen-activated protein kinase kinase</fullName>
            <ecNumber evidence="8">2.7.12.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_141211</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.2" evidence="8"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376488.1">
        <property type="nucleotide sequence ID" value="XM_007376426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARA3"/>
    <dbReference type="FunCoup" id="G3ARA3">
        <property type="interactions" value="219"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARA3"/>
    <dbReference type="GeneID" id="18870355"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141211"/>
    <dbReference type="eggNOG" id="KOG0581">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_79_1_1"/>
    <dbReference type="InParanoid" id="G3ARA3"/>
    <dbReference type="OMA" id="IFVKSCL"/>
    <dbReference type="OrthoDB" id="10252354at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031416">
        <property type="term" value="C:NatB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005078">
        <property type="term" value="F:MAP-kinase scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:protein-N-terminal amino-acid acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007232">
        <property type="term" value="P:osmosensory signaling pathway via Sho1 osmosensor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038066">
        <property type="term" value="P:p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000341">
        <property type="entry name" value="MAP kinase kinase PBS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF25">
        <property type="entry name" value="MAP KINASE KINASE PBS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="334"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="20"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="176"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="674" mass="73542" checksum="430CE4B562BF1945" modified="2011-11-16" version="1">MPPSSHVHLNSDIQARLMAFQQKRTKLGSEANNSATALSPSLSGSTNSSSINNSGNCSRDELSRISPLEKPLPPLPESAPPDYVKRSLSTNDKYARAAAYGINENANDIIKSAASLSGSTSSPTLPTYSIPTRGNSVSARLPALASPKNVPTSIDLKKKPSLSQRRGMKLNVGDLAIGNTAKSPSPRLLSPPITLSENKTEESKSGGEYIAQAPIASQLGTPGELSRRLSERKKKPHFKLNLDGTNSDIASLSRSSSLNRSNPTTSSSEGPTPESEKNNNNQGESQQLHGLFANYSKYVDIKSGQLNFAGKASLHSEGVDFSSGSSFRISIDELEYIGELGRGNYGSVSKVLHRPTGVLMAMKEVRLELDETKFTQILMELDILHKCNSPYIVDFYGAFFVEGAVYMCIEYMDGGSLDKIFGNNIGVKDECTLAYITESVVRGLKELKDKHNIIHRDVKPTNILVNSQGNVKLCDFGVSGNLVASLAKTNIGCQSYMAPERIRTLRPDDATYSVQSDVWSLGLTILELAVGHYPYPAETYGNIFSQLSAIVDGEPPSLDPEIYSSKARAFVKACLQKKPELRPSYAELVEHPWLQEYRGKDPHLDATVKNRLTEIEAENTQRNSNKVNSMKVANIPMQNIENVQSLLRNKVKAPALHRGGLQSINRSFLNHDKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARJ0</accession>
    <name>G3ARJ0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_140326</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376089.1">
        <property type="nucleotide sequence ID" value="XM_007376027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARJ0"/>
    <dbReference type="FunCoup" id="G3ARJ0">
        <property type="interactions" value="1091"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARJ0"/>
    <dbReference type="MEROPS" id="T01.971"/>
    <dbReference type="GeneID" id="18870285"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140326"/>
    <dbReference type="eggNOG" id="KOG0182">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_4_1_1"/>
    <dbReference type="InParanoid" id="G3ARJ0"/>
    <dbReference type="OMA" id="YGYDMPV"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03754">
        <property type="entry name" value="proteasome_alpha_type_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000026">
        <property type="entry name" value="Proteasome subunit alpha type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034642">
        <property type="entry name" value="Proteasome_subunit_alpha6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 5 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="7">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="247" mass="27295" checksum="638A4A46AEF021A9" modified="2011-11-16" version="1">MSSSAAGFDRHITIFSPEGRLYQVEYAFKAINSSNITSMGITGTDCAVLISQKKIPDKLLDPATVSYMFKITPSIGLVATGSIADARAQALRARSEASEFRYKYGYEIPVEGLSKRLANLSQLYTQRAYMRPLGVALTLCQFDEEEGRGPQIFKCDPAGYYTGVKAVATGPKQQEATTYLEKKFKKVDCINGDWKETVEFSIIALSNVVGTEFRKNDIEIGVASKDGFRILSPEEIDERLVAIAERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARK9</accession>
    <name>G3ARK9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Brl1/Brr6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_51742</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376540.1">
        <property type="nucleotide sequence ID" value="XM_007376478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARK9"/>
    <dbReference type="FunCoup" id="G3ARK9">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="GeneID" id="18871589"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51742"/>
    <dbReference type="eggNOG" id="KOG4503">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1046505_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARK9"/>
    <dbReference type="OMA" id="RINHEEK"/>
    <dbReference type="OrthoDB" id="5961at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040202">
        <property type="entry name" value="Brl1/Brr6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018767">
        <property type="entry name" value="Brl1/Brr6_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136:SF5">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10104">
        <property type="entry name" value="Brr6_like_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01042">
        <property type="entry name" value="Brr6_like_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Brl1/Brr6" evidence="4">
        <location>
            <begin position="90"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="233"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW31762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="266" mass="30502" checksum="076FFB0A2E4A1AE2" modified="2011-11-16" version="1">MSSEDFNKNITNDKQDGDMPINNSSIRSKATFLDVSPDNKESDSIDNQPQLQMQNPIEQQVLSSNTIPVEYQRESLVREVLSDPRIPYTLALYLQLISNAVLTSIIFYLIYIFITTIRIDINQRMQSTALEILQEISFCSREYVRNRCSPESGLRVPALEQECTELDKCRNRDPQLIARSKITAETFAEIVNGFIGKISWKSIILINILIFGGLIVSNVAFGNYRTHSAPFSSKEDQEKINQLEKKVREQELIIRNYENGDQRQVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARN8</accession>
    <name>G3ARN8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription initiation factor TFIID subunit 12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_62384</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF12 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376569.1">
        <property type="nucleotide sequence ID" value="XM_007376507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARN8"/>
    <dbReference type="STRING" id="619300.G3ARN8"/>
    <dbReference type="GeneID" id="18874300"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62384"/>
    <dbReference type="eggNOG" id="KOG1142">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021602_1_0_1"/>
    <dbReference type="InParanoid" id="G3ARN8"/>
    <dbReference type="OMA" id="PANEMAQ"/>
    <dbReference type="OrthoDB" id="2193432at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07981">
        <property type="entry name" value="HFD_TAF12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037794">
        <property type="entry name" value="TAF12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003228">
        <property type="entry name" value="TFIID_TAF12_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12264">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12264:SF21">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03847">
        <property type="entry name" value="TFIID_20kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 12" evidence="7">
        <location>
            <begin position="350"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="68"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="201"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="77"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="109"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="228"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="469" mass="51110" checksum="DD8737D1811D1626" modified="2011-11-16" version="1">MEGSSNIDPPKVTPDPEINQQKAKILLQKLKEEIAQAKATTDPAKAKQHYQVAEKIKRVLLNYQASQAKKDGTDTASAVPVTGPSTVPPSAGESRSTSPEIKSPVPQRPSSVPAPTTTSAATPPVNNELQGQKITIERFNHVKNRMKEVYEGVKKLEQAHSDEKDAAKKQTINEQMERLKGQLVSFQKLAVYMKNQLVQQGRISGSTTPVNVNTSPTPVQPPQTQAPAPAPAPQQPQQAPPPPQEISSPAKTKSIKKEEPAVVSPQKSKSKSITPASVTTSITPKPDSVRPELAKSVSVKLNMTKPTITPSITSASTTHLSSLARPDISQLSSNTITPNNIPDNGGRVLTKRKLVELINNLGVDQGDSKMTIDGDVEDLFLDLADDFVRSVVGFSCRLAKHRKIDRIDIRDLQVNLERNWGLRVPGYSSDEIRAARKWASSDEYSDVAKRIEQENRGPIVGKKEKKNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARV7</accession>
    <name>G3ARV7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_141249</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376152.1">
        <property type="nucleotide sequence ID" value="XM_007376090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARV7"/>
    <dbReference type="FunCoup" id="G3ARV7">
        <property type="interactions" value="520"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARV7"/>
    <dbReference type="GeneID" id="18870357"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141249"/>
    <dbReference type="eggNOG" id="KOG0385">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_17_3_1"/>
    <dbReference type="InParanoid" id="G3ARV7"/>
    <dbReference type="OMA" id="WNICRID"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000015">
        <property type="entry name" value="lymphoid-specific helicase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="173"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="587"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="79"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="833" mass="96527" checksum="19B4D5E58EF16D73" modified="2011-11-16" version="1">MTTSPPIEISDDELHHSESIGELKKLVQEQQSVLIDSHEEQQEFNKLSKDLKFARLNNLIERSQIYSKIIADNILQTSLQNQQQQQPQPEQPKQQPTPEETPPRKKRRTRGSAKPKQDIVSMLSTNISESTKSTRKAIEESQQNTTENKTTQPKLITGCTLKDYQLDGLEWLVTLYQNGLNGILADEMGLGKTVQCIAFLSFLIEQGITGPFLIVVPLSTLSNWYNEVSRFAPKIKVLKYTGNKVERNKINLTSKSSKLNIIITSYEISIKDFNKLSTINWNYLIVDEGHRLKNNECVLIKFLKKLNVSNRLLLTGTPLQNNLNELWSLLNFILPDIFHDLELFQQWFNFDELTSLTTDEEDEETKKLIKFNIQEALIKNLHTILKPFILRRLKKDVIKDLPPKKEYLIQIPLTELQRKIYYDAINSQLFASLIEVNLKEFIKYNHWNLFKNDLHRIDQFLQAVYGGKVTHKEGKHVTSYIEVNTDDEFEEEDSVSIDTSSDEITYEQVLENLPRSKQAKQDAILQSLYRKIYKSIRHLSLQNLMMQLRNICNSPYVYYEPFLIEEGNKNDTKFMEALISNSAKFQALNQLVKPLISQGHKILIFSQFTKLLDLLQDWFHFQEIEVCRLDGSTSQLDRESQISQFNKPGSPESVFLLSTRAGGLGINLTGADTVILFDNDWNPQMDLQAIDRVHRIGQIKPVKIFRFVIRDSIEEILISKSTSKRFLEKLIIQMGEFKFNKLKKLIGGDDKDLELNVKELIELTKYNFKHSSDKENVQDKAPEFTFTDEKYQPVLTTEEVSELLDRSPQCYTSVDDSKFNNITIFETVNNMDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS54</accession>
    <name>G3AS54_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">candidapepsin</fullName>
            <ecNumber evidence="4">3.4.23.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_156595</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential cleavage at the carboxyl of hydrophobic amino acids, but fails to cleave 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17 and 24-Phe-|-Phe-25 of insulin B chain. Activates trypsinogen, and degrades keratin.</text>
            <dbReference type="EC" id="3.4.23.24"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.24" evidence="4"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377046.1">
        <property type="nucleotide sequence ID" value="XM_007376984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS54"/>
    <dbReference type="GeneID" id="18871053"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_156595"/>
    <dbReference type="eggNOG" id="KOG1339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013253_9_1_1"/>
    <dbReference type="InParanoid" id="G3AS54"/>
    <dbReference type="OMA" id="QCYLAIM"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05474">
        <property type="entry name" value="SAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033876">
        <property type="entry name" value="SAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase A1" evidence="13">
        <location>
            <begin position="133"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW31013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="504" mass="54886" checksum="DE1A17EAFD25E48B" modified="2011-11-16" version="1">MIHISDLSMYTHAVRKVYYKMHKSLILQWFLSGAAFAAAPGFVNLDLVSSLGSTGGAQNKDVVATVKSGDELLLTELQIGSNKDSVLVAIDTGSEYLWVMDSDVTCESKPSKRNVFGKRDAVDISLKDKQTQMLTELRIGSNRDKVQVNVDTGSSILWVMSSNVTCAESPLTNKAPKCKAHGTFSYQKSESFNNKKKMFNATYADKESAIGVYSTDDIIIGKDVAVKGFEFGLAVWASSEVGILGIGFPEANSTGRSLPFELSNQGITNRSAYSIFLNKPNKPGSILFGAVDHSKYLGDLQTVPIVYNKTFPEIKVNVSGIDININGTKVSGGISESAFVLDTGTAKSRFPNSVLDTLREQIEGSEYLESEFLFGSPNCSQIENLKIEIKFDGKTITVPVKDLGFDRDGDGCTFDIMFNDDELILGMDVLRSIYFVVDLDDKEVSLAQSDFSNGKQDIEAFPPPGVNRTLASRGRSEQKVSSANSIVSSIDTLLCLAILLTWSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS78</accession>
    <name>G3AS78_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Proline dehydrogenase</fullName>
            <ecNumber evidence="2 5">1.5.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_62929</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Converts proline to delta-1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23784"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.5.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the proline oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.2" evidence="2 5"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377070.1">
        <property type="nucleotide sequence ID" value="XM_007377008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS78"/>
    <dbReference type="FunCoup" id="G3AS78">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AS78"/>
    <dbReference type="GeneID" id="18874529"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62929"/>
    <dbReference type="eggNOG" id="KOG0186">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029274_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS78"/>
    <dbReference type="OMA" id="QECMGFL"/>
    <dbReference type="OrthoDB" id="5464at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004657">
        <property type="term" value="F:proline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.220:FF:000017">
        <property type="entry name" value="Proline dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002872">
        <property type="entry name" value="Proline_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015659">
        <property type="entry name" value="Proline_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914:SF0">
        <property type="entry name" value="PROLINE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914">
        <property type="entry name" value="PROLINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01619">
        <property type="entry name" value="Pro_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proline dehydrogenase" evidence="6">
        <location>
            <begin position="253"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="478" mass="53526" checksum="5087CF7527B14D6F" modified="2011-11-16" version="1">MSLSLYSNAALRRSAVAALRTAPALRSSYIPLRHFNIHPNKRATTTMTNTTTNVPLHTKPEERSFEHLSVFTNKELLSFLIMGLCTLNKPILNTCIKLFPYVPLSIVKALVYRIYCGGETIEEVKTTGMRLHERGINNMMISLTIEACDGNDNVDPEYIVDETCRSVKEILVPHMESMLAKASDVNSIPPGYVALKPTGFAKDSARVLREYNTTSAAEFEGLVEKATKVCQAVYDANIALQKKYPARVAPFTVAVVDAEKHELQEGVYELQRRLYSKFNKMNEPISVVGTLQMYLSESSSLLAKEEQLAVKNNYRVGLKLVRGAYIHSEKNRATIIHQTKEDTDFNYNQGIAYCIDSILHDKGNESTIGHLVVASHNAESLHLASSEVYNESTKDNNNKTNVVLGQLMGMSDSVTFDLINRHQIGNVIKYVPWGPPLETKEYLRRRLEENGDAVSNDNGFKLVKSTSSELLRRIMGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASR4</accession>
    <name>G3ASR4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cysteine protease RIM13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_141771</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family. PalB/RIM13 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377161.1">
        <property type="nucleotide sequence ID" value="XM_007377099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASR4"/>
    <dbReference type="STRING" id="619300.G3ASR4"/>
    <dbReference type="MEROPS" id="C02.008"/>
    <dbReference type="GeneID" id="18870397"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141771"/>
    <dbReference type="eggNOG" id="KOG0045">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023416_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASR4"/>
    <dbReference type="OMA" id="GWLPQII"/>
    <dbReference type="OrthoDB" id="167576at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036213">
        <property type="entry name" value="Calpain_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051297">
        <property type="entry name" value="PalB/RIM13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46143">
        <property type="entry name" value="CALPAIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46143:SF1">
        <property type="entry name" value="CALPAIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49758">
        <property type="entry name" value="Calpain large subunit, middle domain (domain III)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6 8" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="7">
        <location>
            <begin position="144"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="734" mass="84793" checksum="177315C411AA98B5" modified="2011-11-16" version="1">MSLSEVLHLVEYSYLYQSIDDTVKAKKGCLQAIKILNTLAKSDNSTQPHIKEVSQFVLNLYDQIDKQPLNTSGKLSWMSSKLSETGTCPPVISFSPNLNSHNQIFFSEDQAFQDEDKSIQKVPENVQWESVMVDDWSSESSDLTSLYQDILPNCSFVSSFLAIIESQTIPIIQSISPHRPSTQYKVQLMFNGTFRNVTIDNRLPYLSNDRSRNLFISSFKDPRLLWPALIEKAYLKVMGDGYNFNGSNMANDTCLLTGWLPQIIKIENGQLPTEFSRLWELHRQGKVLLGIGTGKLSKQIGLHLNLITDHDYVIDNFRMEGNKKIITIKNPWISKSLDCRLISVDEFTYFKYLYVNWKPEFKYTQNHNFLYSPKEFIFNEPQFTFSNDTAQLQEVWLLLERHLPTDSQWMNMQIFETGSKVLTQTQFKKLGLDSGTNNRIQLIKLNMKPNQSYTAVISCGKSCKLTLSMFNNIGPGFKFNKSKWMYVFTLPPIDGEWTDRTNGGNWSISTFIDNPQYDLIVKKDTKLIVVVYGDTQVNTYLLQSDNSKLGERVHKFDKSKLLNNEHYNTFFQLNNYTLAPGNYKLIVSAYDKYIGKYKLLLNSTSEVQLDKTPFSWGLFTTKTGFNWDNQNRFKLRFSTTSYNTKTIFHITHFNGEHNYELLTNYRPAIRASIFDARNSSPILINEKFDDSLYGIFVDWTLEHPGEYILLIERFELGNGRCVVEIGSSKKVHLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASY0</accession>
    <name>G3ASY0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-alpha-acetyltransferase 30</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">NatC catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_62613</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. MAK3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376795.1">
        <property type="nucleotide sequence ID" value="XM_007376733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASY0"/>
    <dbReference type="FunCoup" id="G3ASY0">
        <property type="interactions" value="462"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASY0"/>
    <dbReference type="GeneID" id="18874405"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62613"/>
    <dbReference type="eggNOG" id="KOG3139">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_0_3_1"/>
    <dbReference type="InParanoid" id="G3ASY0"/>
    <dbReference type="OMA" id="LCIFARH"/>
    <dbReference type="OrthoDB" id="249099at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:protein-N-terminal amino-acid acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000199">
        <property type="entry name" value="N-acetyltransferase catalytic subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044542">
        <property type="entry name" value="NAA30-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45896">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45896:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="6">
        <location>
            <begin position="11"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00071796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00078622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="182" mass="21647" checksum="2A9D1FB1F9621D1E" modified="2011-11-16" version="1">MSNLLRTINNLTYHRFDINDQDEFKQISKLIAIHLSEPYSIYVYWYFLNNWPQYCYTVKNDKSVIIGVIISKIEPHREVRMRGYIGMLVIDPEYRKMGIASNLVKLTIENMTQDNVDEIMLETEVINEGALKLYESFGFLRTKRLYRYYLNTHDAYRLILPLTDKAHTRIAFLPQLPQEQVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT17</accession>
    <name>G3AT17_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">beta-glucosidase</fullName>
            <ecNumber evidence="3 6">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">BGL2</name>
        <name evidence="8" type="ORF">SPAPADRAFT_141816</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Glycan metabolism; cellulose degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="3 6"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377210.1">
        <property type="nucleotide sequence ID" value="XM_007377148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT17"/>
    <dbReference type="STRING" id="619300.G3AT17"/>
    <dbReference type="GeneID" id="18870400"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141816"/>
    <dbReference type="eggNOG" id="ENOG502QR4D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004542_4_0_1"/>
    <dbReference type="InParanoid" id="G3AT17"/>
    <dbReference type="OMA" id="EFGCSCL"/>
    <dbReference type="OrthoDB" id="47059at2759"/>
    <dbReference type="UniPathway" id="UPA00696"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.300:FF:000006">
        <property type="entry name" value="Beta-glucosidase H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000495">
        <property type="entry name" value="Periplasmic beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050288">
        <property type="entry name" value="Cellulose_deg_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019800">
        <property type="entry name" value="Glyco_hydro_3_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011658">
        <property type="entry name" value="PA14_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715">
        <property type="entry name" value="BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715:SF27">
        <property type="entry name" value="BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07691">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00758">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00775">
        <property type="entry name" value="GLYCOSYL_HYDROL_F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PA14" evidence="7">
        <location>
            <begin position="405"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="841" mass="93116" checksum="9EFE78ADAF939AB6" modified="2011-11-16" version="1">MLRQLDIESLIEELTLSEKISLLAGYDFWHTVPIERLNIPSVRVSDGPNGIRGTKFFNSVPSNCFPCGTALAATFNSDLLVSVGELMGKEAKMKGAHVILGPTCNIVRGPTGGRAFESYSEDPILSGHAAASVVKGIQNENVVACLKHFVCNDQESERKAVDVIITERALREIYLKPFQIAVRDAYPKAMMTAYNKINGVHVSQNKKILQDLLRKEWNWDGTIMSDWHGVYSTKESLDAGLNLEMPGPTRFRQELQTTHAIQTNEIHRDVIDDNVRQILKLVNESFKAGIPDDVVESPNPSKEAEELLRKAGDESIVLLKNNENILPLPKTATGSIKKIAVIGPNAKAAQDSGGGSASLNARYKITPFEGIKSKLNDGSIELTYALGAYLDRNLPDVGQLLVNDKGEKGVTGKFYKNEPGAQDRELFEEFLLPTSRLFLSDFKSDKLDRNQLLFYADFSGYYIPEESGEYEFGCSCLGTAQIFVNDKLIVDNKTKQVKGDAFFLGLGTREERGSLKLEKGIKYEVRVEFGSSPSYTLNKAALEGGGVFFGIQKKATPEEAIKEAVQVAKDADVVVLVVGISKEWESEGFDRPNMDIPGHTNQLVEEVTKVNPNVIVVNQSGSPVSLPWINNVKGFVQAWYGGNELGNTIADVLFGDYNPSGKLSMTFPVRLEDTPAYVNFGSTHGQVLYGEDVFVGYRYYEKVGKQVLFPFGFGLSYTNFEFSNLHVTSDEENVYATVHVSNVGKVDGAEVVQLYVEQEDPVVMRPVKELKAFNKVFLKAGETKKVELSVSIKEATSYWNGYKNKWQSTKGKYNVFVGNSSDNACLTGEFETEKTYFWLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT34</accession>
    <name>G3AT34_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="5">3.2.1.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Endo-1,3-beta-glucanase btgC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Laminarinase btgC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">SPAPADRAFT_56764</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Glucanases play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation. This enzyme may be involved in beta-glucan degradation. Active on laminarin and lichenan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="5"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376830.1">
        <property type="nucleotide sequence ID" value="XM_007376768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT34"/>
    <dbReference type="STRING" id="619300.G3AT34"/>
    <dbReference type="GeneID" id="18872065"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56764"/>
    <dbReference type="eggNOG" id="ENOG502QTKT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_391894_0_0_1"/>
    <dbReference type="InParanoid" id="G3AT34"/>
    <dbReference type="OMA" id="DAIEYMN"/>
    <dbReference type="OrthoDB" id="68336at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050732">
        <property type="entry name" value="Beta-glucan_modifiers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631:SF17">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BTGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00587">
        <property type="entry name" value="GLYCOSYL_HYDROL_F17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0134">Cell wall</keyword>
    <keyword evidence="14" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="20" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="10 20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="330"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="239"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="247"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00037649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00043078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU004336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="EGW30797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="704" mass="78743" checksum="31124E2474C2B37F" modified="2011-11-16" version="1">MTQLQGYLHRRIFIASVDMSKMDAANFQNVFPPKRSATISEFQPLSSGSFTEGGGQKNENPEQPKLTKVSPYKITRSRSINTFNEYRRSSEISPIAFTRPLKSGQNQDLPQSRSSQINKEPTVLKITGNSIDDDNAETPITLGQSSLNIPAYYCLDQSALHGSLLSGAIPIASGTNTSSATRNATVIFRKKNKSPYESDYNLTAISPISDESAPFKPMRKTSTQIFSGPIIKEEDVEEINLNDDENNTDKNKTKPSYDQKKDDGTISSVLHPLVYNKSILGKPTFSEEDSIKNMDQLSESLSKRSPNNGGKQPRFNWVPAFPSFKIKGSIIIHIFLVLVIISLLCGFIPAVVILSKGTKSSVDNYFSQSTNLPHLSELSDKYLHTSITPEPIPRINSVQGLNDDVKNDTEVINLMGVVSNLMFHGIAYSPNNAMEPDCGYTKKDAMLDLAKLSTVTTRIRNYGMQCDQTEIILDAIEHMNLNMTLAMGVWIGSNNSVNRKHMDTMKKIIAKHPHPSRLINSIYIGNEVLFRGDKTRDELIEYIQDAKEFLKLMHIDDIPVGTSEIGSLIDKELLQACDIIGANIQPFFGGVTVEDAVNWTLDFFQYQIEPYNEKNIPVVVTEVGWPSGGGKFKGAIASRAGLSYFVNEFLCAFNDLGIDYYFFEAFDEPWKEIFWDGNRKWESQWGIFKADRSNKFNLQRAPCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT76</accession>
    <name>G3AT76_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_52038</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376872.1">
        <property type="nucleotide sequence ID" value="XM_007376810.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AT76">
        <property type="interactions" value="856"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AT76"/>
    <dbReference type="MEROPS" id="C19.103"/>
    <dbReference type="GeneID" id="18871621"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52038"/>
    <dbReference type="eggNOG" id="KOG1870">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001060_7_1_1"/>
    <dbReference type="InParanoid" id="G3AT76"/>
    <dbReference type="OMA" id="PYCEKPE"/>
    <dbReference type="OrthoDB" id="292964at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2230.10">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035927">
        <property type="entry name" value="DUSP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF24">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143791">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DUSP" evidence="10">
        <location>
            <begin position="60"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="337"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="814"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="866"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1209"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="821"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="867"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="882"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="915"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1214"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1250"/>
            <end position="1273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1276"/>
            <end position="1287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1287" mass="146362" checksum="456DEC363E8A0FD1" modified="2011-11-16" version="1">MTFNQTNDQVLTNSHESNTDIQTAIQSIKENLQQSSELTLEQQQPSATSDQDSTDLNHFDDLTEKRQLINELLLDNRNTGKEGDEFYLISSEYLTKFLNLPVDNLYELKQQLGPINCTSIVDSLGNLYPENNEPIPTYNISKEIFEHLSSWMGVVGNPVSRNLIINSDTGAKEVERYPVTFLVHQLGSPKRQQHITSHFRSSHRPQAPPPPPPPVPIALSRTKTFNDLIETIRTNIFKTSKKSIISDFRIWFIDEDSLPFHIPISAFVYEIGQKSLVTHSLFGSTIKSQGITKGTYDILIEVKERNHFPLDQFVLSHSNAVTTAAGAASTSTSGGHLGLSNLGNTCYMNSALQCLLHVPEINFYFYYNIYKQELNVNNPLGYHGNVANSFGSLLKQAFDKRGSGSGSISPRDFKSTIGRYSSMFSGYLQQDSQEFLSWLLDALHEDLNRIHEKPYCEKPELNDDEINDSKAIIELSKTCWNQHLARNDSVITDLFTGLYQSTLVCPDCSKTSITFDPFNDLTLPLPISKKWYHTFTIVDLSPAETKTGTRIMKLEVELNKTSNFDDLLNYLGNFLKTPTTNLFIYEIFHNSFYSDFQRDYIKNRFHPIGDIIRDNDEIIVYIIPHDPESDIIVPVFNAVENADKSYKVTNIFGIPLFVVLNKEKEAISFGQVRKKLVEVASVLTTVDLVDEYETIKQSKPGYIAKEYYNKHDFTDVASISDGEESEGYDSDVSLANPYVDGDFGFTMMYIHDYNSHKLTSSSYFRSKFSKSKNENDNSQGLTINVPLHKPNFHEFKSLADQLPENKRNYYHHSKVVNASEEVQEETDKADEEDAVAAESEDSSTTDEEFVMVDPKVNVPPVVPIIPPSVSESVEEQLSAEVAVDDEEEEEEETENEMAVGSLFDSTANLPLAPGSESTIPSNVNSPRSPEDSGEDEQRPLVTKDTILLCDWDNEIFQRCFGEEELTTWNEIPSLSNPELEQNRAHFERQRKAKISLYDCVRSFGTPEVLGEHDLWYCPRCKEHKRATKTIQIWSTGDILTIHLKRFHSARAFSDKIDILIDFPINGLDISSYVSDPIQTDCVYDLIAVDNHYGGLGGGHYTASVKNFRDNNWYYFNDSRVTRIDSAEEVITNAAYLLFYRRRSTNEFLGSEKLNYMLEKGRDQYNQELVNRQASIDVIAEQIKQYGEFESNLIKQIELDKELESRKKRNAQFNLDEEQLDSSEEEEEEEETSEEESDDNDNVRKQRLISKGNNNSKLIQIKSNGRQEVTSSPINLEESDGDLYDGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATE0</accession>
    <name>G3ATE0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_62816</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376936.1">
        <property type="nucleotide sequence ID" value="XM_007376874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATE0"/>
    <dbReference type="STRING" id="619300.G3ATE0"/>
    <dbReference type="GeneID" id="18874478"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62816"/>
    <dbReference type="eggNOG" id="ENOG502QRM1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_703149_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATE0"/>
    <dbReference type="OrthoDB" id="4096729at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052400">
        <property type="entry name" value="Zn2-C6_fungal_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47657">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN ECM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47657:SF7">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN ECM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="49"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="393" mass="42060" checksum="7D9063EF6F0C9572" modified="2011-11-16" version="1" fragment="single">MTENSTSRKSSIDHQEHHDEDHSQSPDAHRGKNRTSTGKRKYHSKSRNGCSTCKRRRVKCDEVRPICTKCKNLKLDCGYLHDDSNQNSDSHHQQQQQQQQQQPSPPTKKRKFSKSGETIKTQQATPSLTPSPSVIHDKVKTESVTAAVPEPSLAQSMQQTQQPQPQQAPPNNTLNALASGLLSAGNLNNINVARLVNDLSSMSDLSALSNLGSMGNLANLSSLATLAQFPIDLSNLGSLLDSGGLAAGMAAAATAAAAARIVPPTSSSSATSTAMPSASSSTHGNNNTVTPLTPNTSSSLLSEIEAEQQQMGYANLAMPKNNITDLPDSNQANGEENGSVPPSIPQSNLNMQDLRLMFHYTSQVASTITGAGISDTNIWNFDIPMLAFNYPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATH7</accession>
    <name>G3ATH7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_62851</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376973.1">
        <property type="nucleotide sequence ID" value="XM_007376911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATH7"/>
    <dbReference type="STRING" id="619300.G3ATH7"/>
    <dbReference type="GeneID" id="18874491"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62851"/>
    <dbReference type="eggNOG" id="ENOG502QTKQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_571067_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATH7"/>
    <dbReference type="OrthoDB" id="1924787at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF12">
        <property type="entry name" value="REGULATORY PROTEIN CAT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="3">
        <location>
            <begin position="3"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="329"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="341"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="351"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="451" mass="50791" checksum="8F348A79C8899592" modified="2011-11-16" version="1">MQAERRILFWCIYCLDVYSSLNLGIPRLLKDYEIECAMPFAGKNGDDDDDDNVNILIVNNTALSIVGKVSRFSLSVMLYCKVLATILDSIYSRFETGDIQRKAVTRDRTLDCWRRDLPADLKFEMDINGLSLKDGQNHYIDENSLWQNYNKQQLTLIFLYYHAKILIYLPIISKYGNHHDVGLSQKEQLAKQSEMADGHDGLGPNTAVVSSVSLIQQSTIQILEVLKCISNNIQSTILPIPINITREQSLFALLVAKGTLDYIKGGPLFNKSKQLLLDTIQNMSIESRIDVPGGLHKNSFRLLELALLSILNMSKSTRRKISPHQLPITKTAVERDPRNFRNGNSGVSYSQQIPQQQQQQQQQQQQQSVHATLPSQPLPVQPVHYSNLDMSNFSDILDTDLDNLESILQFDPYKVNLSSSVVGEFAADGSLGLVPFLDMTDSVMSDDKFTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATN2</accession>
    <name>G3ATN2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 17">Dihydroorotate dehydrogenase (quinone), mitochondrial</fullName>
            <shortName evidence="17">DHOdehase</shortName>
            <ecNumber evidence="4 17">1.3.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">URA1</name>
        <name evidence="19" type="ORF">SPAPADRAFT_72880</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>(S)-dihydroorotate + a quinone = orotate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:30187"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="17">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 17">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Mitochondrion inner membrane</location>
            <topology evidence="1 17">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.2" evidence="4 17"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377026.1">
        <property type="nucleotide sequence ID" value="XM_007376964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATN2"/>
    <dbReference type="FunCoup" id="G3ATN2">
        <property type="interactions" value="724"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATN2"/>
    <dbReference type="GeneID" id="18875461"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72880"/>
    <dbReference type="eggNOG" id="KOG1436">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013640_4_0_1"/>
    <dbReference type="InParanoid" id="G3ATN2"/>
    <dbReference type="OMA" id="IYGTDTR"/>
    <dbReference type="OrthoDB" id="14784at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00946"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106430">
        <property type="term" value="F:dihydroorotate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04738">
        <property type="entry name" value="DHOD_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000066">
        <property type="entry name" value="Dihydroorotate dehydrogenase (quinone), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050074">
        <property type="entry name" value="DHO_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005719">
        <property type="entry name" value="Dihydroorotate_DH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003645">
        <property type="entry name" value="PRK05286.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003652">
        <property type="entry name" value="PRK05286.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01036">
        <property type="entry name" value="pyrD_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109:SF4">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00911">
        <property type="entry name" value="DHODEHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 17" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 17" id="KW-0288">FMN</keyword>
    <keyword evidence="15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9 17" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Dihydroorotate dehydrogenase catalytic" evidence="18">
        <location>
            <begin position="86"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW30993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="414" mass="45000" checksum="7712060A611C52DC" modified="2011-11-16" version="1">MSIYCLYLQSSIIPGPILFIGGIGVVVAGGYYMLDARSAFHEYVLCPLIRTFTDGEQGHKLGIFFMKYGLSPRLLDEGINDQSDVLGVEVFGTKLRNPIGLAAGLDKDGEAIESLFNCGFSYVEIGSITPEPQPGNPKPRFFRLPRDDAVINRYGFNSSGHFNVLATLKLRFNKLFNANAPSNAFKDGKLLGINLGKNKFGDEISDYTKGVERLGPYADVLVINVSSPNTPGLRDLQSEEKLTNLLTTVVKQRDTLGTNLLGKKPPVLVKVAPDLTEPEIESIANSAKEARVDGIIISNTTIQRPVEKLLTTDSQLINQTGGLSGKPLKPIALQALRTLRKYTKDSNLVLVGCGGISSGKDALEFGKAGATFIELYTAFAYKGPGLVAKIRDELAAELRKEGKTWEQIIGEENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATP6</accession>
    <name>G3ATP6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_143367</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377243.1">
        <property type="nucleotide sequence ID" value="XM_007377181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATP6"/>
    <dbReference type="STRING" id="619300.G3ATP6"/>
    <dbReference type="GeneID" id="18870540"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143367"/>
    <dbReference type="eggNOG" id="KOG2189">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005230_0_2_1"/>
    <dbReference type="InParanoid" id="G3ATP6"/>
    <dbReference type="OMA" id="TYVQLYI"/>
    <dbReference type="OrthoDB" id="10264220at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF59">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A, GOLGI ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="513"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="557"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="644"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="673"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="737"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="901"/>
            <end position="924"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="963" mass="109986" checksum="443D98AEE48F589C" modified="2011-11-16" version="1">MSPFRLFSSTTSDTNIAPLKLNAHTAPEEAIFRSAPMTLVQFYVTIELARDMVWALGNLGNVQFRDLNSKLTPFQRTFVNDLKSIDIMSTKLHQLFQIMVRHETIKTDLVGAYLHADLKPLPAAAAMDDLKQKLDEFHDRIKHLDLSFNNLNRQKLRQVENRNVVNIVDDFHRSSLVTEVERHSLENRASIEDDSVALLNDQQRRNQSLELGLQEADLEDGAFDSIAGTIARDKVPILRNILWRTLRGNLFFHDIQIDEQFPVNDSSDELIDKNVFIIFIHGDLLKSRVRKIIQSLDGVIFDNVVGGADTRSATLIELNNKIEDLNSVVVSTKQQLITELKIFQESYPDYCYIIEREKLIFETLNKFDEDSTRRCLVGEGWIPKSEFTKIQSTLRNLVKEKTRHANAGLTASSNESVALSSTGTLETQTSLFAIDDTTSDHDISRFEIGDEDDEDDYGTLIAVVNELSTNRTPPTFHRTNKFTSAFQSIIDAYGIATYQEVNPGLATIITFPFMFAIMFGDVGHGFIVLLGALYLIKNEISFGAMRNKDEIFEMAFNGRYIILLMGFFSIYTGLIYNDIFSKSIQIFSSGWKWTFPKGYDFAKDGAVTLIAEKISGKVYPFGLDWAWHGTENNLLFTNSYKMKLSVLMGYTHMNYSLMFSLVNYLYFKRKVDIIGNFIPGFLFMQSIFGYLSLTILYKWTVDWFGTGRQPPGLLNMLINMFLSPGTIEEQLYPGQKFIQIVLVLIALVCVPWLLIYKPLTLKRENDKAIQLGYSDVHSQRHHSFQLHEEERALEFEQELNNDPNDDDDDSFLADDEFRFPNDIEPMFHSAAGHGDDHDKFNFGDIVIHQVIHTIEFCLNCVSHTASYLRLWALSLAHAQLSTVLWSMTIQNAFGASKNKTIGIIMVVVLFAMWFSLTVCILVLMEGTSAMLHSLRLHWVEAMSKFFEGEGYVYEPFTFKEIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATT8</accession>
    <name>G3ATT8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="4 15">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 15">Tyrosyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_63163</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="4 15"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377285.1">
        <property type="nucleotide sequence ID" value="XM_007377223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATT8"/>
    <dbReference type="FunCoup" id="G3ATT8">
        <property type="interactions" value="674"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATT8"/>
    <dbReference type="GeneID" id="18874625"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63163"/>
    <dbReference type="eggNOG" id="KOG2623">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024003_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATT8"/>
    <dbReference type="OMA" id="FKLYCGA"/>
    <dbReference type="OrthoDB" id="337870at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070184">
        <property type="term" value="P:mitochondrial tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000001">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000158">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024088">
        <property type="entry name" value="Tyr-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766:SF0">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766">
        <property type="entry name" value="TYROSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15 16" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 15" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW30314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="511" mass="57574" checksum="2868163116234804" modified="2011-11-16" version="1">MLLRTSHRTPSVLLRHLVKRYNSSPSSIDLSKRDTVTIIPSVYELTQDKAHTSETDTSLVSYLQARHLVESITSDDLYEITEPDADVKFKLYCGADPTAASLHLGNLLPLMILLHFRLRGNDVVGLVGGATGKVGDPSGRETERTQMERAERETNIIKIQSQLSGFLNKGIEYAKSRQFPQHEGEVQVVNNADWWSDIKMLDFLADYGRFIRISSMLARESISARLQGQAGIGFNEFTYQILQAYDFWHLHKHHGVNMQVGGNDQWGNITAGIDLISRLQRQTNSEPTPVYGLTVPLLTTPSGQKFGKSAGNAIFIDAKLTTPYQMYQYFINVPDDMVEKLLKIFTLLPLSVIESEIIPRHEQEPGIRLAQRILAREVVDLIHGVGVGDEMAYITGFLYPTPDQPFNDVVSADKLIETFKKSGILKTFKLSDFENIDDIKLSSVLAKVTGNSRREIKNLIKSGGVYLGLERDQFEDPDDVVLFDRENHLIEGKLLLVRLGKQKYFVVEFTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATU3</accession>
    <name>G3ATU3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16 17">Ketol-acid reductoisomerase, mitochondrial</fullName>
            <ecNumber evidence="5 17">1.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15 17">Acetohydroxy-acid reductoisomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14 17">Alpha-keto-beta-hydroxylacyl reductoisomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ILV5</name>
        <name evidence="21" type="ORF">SPAPADRAFT_63175</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-acetolactate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22068"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
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        <reaction evidence="17">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-ethyl-2-hydroxy-3-oxobutanoate + NADPH + H(+)</text>
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        </cofactor>
        <text evidence="17">Binds 2 magnesium ions per subunit.</text>
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    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.</text>
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    <comment type="subcellular location">
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            <location evidence="1 17">Mitochondrion</location>
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        <text evidence="4 17 18">Belongs to the ketol-acid reductoisomerase family.</text>
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    <sequence length="401" mass="44544" checksum="27E37A0AFA52516D" modified="2011-11-16" version="1">MSARTASIRMARLATARASAQIASKRSLSALAATAIRRPVIGSIAKTMIASRGVKTINFGGTEEVVHERSDWPREKLLDYFKNDTLALIGYGSQGYGQGLNLRDNGLNVIIGVRKNGASWKAAIEDGWVPGENLFDVTEAIERGTYVMNLLSDAAQSETWNTIKPLITEGKTLYFSHGFSPVFKDLTHVEPPTNVDVILVAPKGSGRTVRSLFKEGRGINSSYAVWNDVSGKAEEKAIALAVAVGSGYVYQTTFEREVNSDLYGERGCLMGGIHGMFLAQYEVLRENGHTPSEAFNETVEEATQSLYPLIGKYGMDYMYDACSTTARRGALDWYPRFKDALKPVFEDLYESVKNGSETKRSLEFNSQADYREKLEAELKTIRDMEIWRVGKEVRKLRPENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="48" xmlns="https://uniprot.org/uniprot">
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            <taxon>Ascomycota</taxon>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="655"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="813" mass="90356" checksum="08B2D71BCC1A1501" modified="2011-11-16" version="1">MDFEERLEEAEQLDAPSLVGSSLRSADDSIHNNYIHPEKYHSTAKSSSSSKFSQSISASLDDLINEGALLASEEDFDNYLDTDGSVKPDIKFEPIEEKADEENSDDKDETEKVKQPETKETDDVEESNESNTASGIPSSPVQVSEAVTGAEQEPTSTTGNSLYENENYSTPNLSEYQLENEIKDHSQLLESVKSYDHNKLPRSEASTREHSAGFSPAAKKSITTPQAVPQRTEGLVAVSREGIHSPFFYTDRPRSRSRSANPPGSRSGNRSSSRSSSSLKPHLARGDSYKNTNVDEPSKYELPPDLALQKEKEEGDSESVSVQSYEGEVEEEEEEDRRTRYSRPTMGESIAAAEAAAAALKAESSTFKANAPPATQFGMGNYDEKPITRDPSLVTTGDYTNFEVDSPRNALPPTSNLYGARSASSTNYLRSISRSRSRARTTEIAHGPSLDDKNDANPHQLQQEGALISDDPYASIDELDTMVEEVLHGKPANEEEEESKKEILDKVGGIGLDDDKEPPSVVIDEPAKEENDKEKVKAEKEEEQQVTKEEKTDEEPKAKEEVIEPESKEVEEVIEPESKEAEKVIEPESKEAEKVIEPESKEVEEEAVLVEKPTDSEKEKEHENVAVEEETESKTKEVEVAPVAKEDDITPVGNKTEEPFPVEKEVKSESKELEVTPVEKEAESEPKEEPEVETTAPKESPVVDDDLDDLDISPEELRKHLESLPIYLFTSLAGGMQIMPRTNRLVTILNANGIKFEYRDLGTDEEAKKIWKRQAGGKTLPGIVRGDDFIGNWHDIDEANEEYKLHELLYETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU57</accession>
    <name>G3AU57_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
            <ecNumber evidence="7">4.2.1.93</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ATP-dependent NAD(P)HX dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_143283</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(6S)-NADHX + ATP = ADP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19017"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>(6S)-NADPHX + ATP = ADP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32231"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.93" evidence="7"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377404.1">
        <property type="nucleotide sequence ID" value="XM_007377342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU57"/>
    <dbReference type="FunCoup" id="G3AU57">
        <property type="interactions" value="174"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU57"/>
    <dbReference type="GeneID" id="18870532"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143283"/>
    <dbReference type="eggNOG" id="KOG3974">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030651_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU57"/>
    <dbReference type="OMA" id="WRAAYHN"/>
    <dbReference type="OrthoDB" id="8110916at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047453">
        <property type="term" value="F:ATP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110051">
        <property type="term" value="P:metabolite repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017953">
        <property type="entry name" value="Carbohydrate_kinase_pred_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01050">
        <property type="entry name" value="YJEF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF C-terminal" evidence="8">
        <location>
            <begin position="10"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="199"/>
            <end position="205"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="242"/>
            <end position="246"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="261"/>
            <end position="270"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW30433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="355" mass="38936" checksum="3DDCC35FB591BD93" modified="2011-11-16" version="1">MLRNKSPKELINISRNLVQPLLPHFHKGQSGKVTVIGGNEDYTGAPFFACHSAALVGADLSHVICERHAAPVIKSYSPDLMIHPYLYDLDNPAIKLAQAEIDQLRKTPLKDVITDGALLNSIIDDLVMPKVKGLLARTDIVVVGPGFGRDPLMMKSLVRIIDEIKQMDLPIILDADSLFLISIYPNIISGYKKAIITPNVVEFDRIAKTLGIDSSISEQNVNTLIEETQTMSKKLGVTVIRKGRQEVIAYQDTYLVNDFSGSSRRVGGQGDTLTGSLATFVNWSYNYLDKVWDTKGDISEGDAQVLACYAACSLVRSSAAKAFAKHGRAMQTTNVHECLPTAFAELFESNQKANI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU89</accession>
    <name>G3AU89_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCA7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_63294</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377435.1">
        <property type="nucleotide sequence ID" value="XM_007377373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU89"/>
    <dbReference type="STRING" id="619300.G3AU89"/>
    <dbReference type="GeneID" id="18874687"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63294"/>
    <dbReference type="eggNOG" id="KOG4140">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1077683_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU89"/>
    <dbReference type="OMA" id="KSHAIML"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031048">
        <property type="term" value="P:regulatory ncRNA-mediated heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904802">
        <property type="term" value="P:RITS complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013243">
        <property type="entry name" value="SCA7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037804">
        <property type="entry name" value="SGF73"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47805">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47805:SF1">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08313">
        <property type="entry name" value="SCA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCA7" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="255" mass="28898" checksum="F35F264B70F440C4" modified="2011-11-16" version="1">MGAKRAVPGRSAPYDVLLQQYQKRNQAKIAQSHALAAQKRENAAFHGGEHDENSANYNKILDPDEETHLVLEGLSKNNPIPLERKIIMPVRYRHRFLQAREFYANALIISPHHHHNQQQQQQQQAGGEQTLANQAISGSIGGLQGRCALINVDAAPPANTDSPTQTFSAISGEMYQVRAPSKAILMTSQHNNMQQQAFQQQLLKYQQQQQQQQQQLLLMKQRQLQQQQQQQQQQQQQQQQQQQIQSSSQGSSVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUB6</accession>
    <name>G3AUB6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_52572</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377463.1">
        <property type="nucleotide sequence ID" value="XM_007377401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUB6"/>
    <dbReference type="FunCoup" id="G3AUB6">
        <property type="interactions" value="182"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUB6"/>
    <dbReference type="GeneID" id="18871694"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52572"/>
    <dbReference type="eggNOG" id="ENOG502QQ4K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020873_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUB6"/>
    <dbReference type="OMA" id="TKLWGYE"/>
    <dbReference type="OrthoDB" id="21643at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="3"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="345"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="360"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="521" mass="59287" checksum="80C430963EEA0A52" modified="2011-11-16" version="1">MTTRIHIGSISPNLQSNPDSLTTRLEKFGKVVTPLSFHTKPTADHYFAYVTLDITSANFDKLKGQLNGVTFMGRKLSVSIAKPSYLDQVSKPDHTKQERITRDKISQIRQARITDGHTQYKQTTSGEICTAPLVTGSWSVSPHTFENKSANTKNKPPSHNLVGSKSYGAWTNARSSFDKAYSNISGRGEVIKARHRTTPRKDIRHQTLRLLINDELKLIKCYKTKLWGFDKKPLKELSFRYESGQWINGDGELIERVDKFSEEAPVDDVEEHDRNKSLLASIDFSKPAEIEGEDGVSEEEEEEEIYVNRQVMDGPVEGELAAPVEEVYYDEDDEGNDVDFDAINQVSVPRGETEEKYEPVAEEEAEAEAEEDRPQEPAVAETPDTNNLDTLRSLFNAPSQSTFKLELSDDDIEETTALPNETTQQQILEQIQAKQETYAVKATKFGLFWPHLDSPFLSTQSQLNKIGSVHDKIVLPGEDEDMSEGEETKYEKWFWNNRGEISRMCKRRRRDVLRIMSKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUD5</accession>
    <name>G3AUD5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_143089</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BMT family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377482.1">
        <property type="nucleotide sequence ID" value="XM_007377420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUD5"/>
    <dbReference type="STRING" id="619300.G3AUD5"/>
    <dbReference type="GeneID" id="18870518"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143089"/>
    <dbReference type="eggNOG" id="ENOG502QTZG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013841_1_1_1"/>
    <dbReference type="InParanoid" id="G3AUD5"/>
    <dbReference type="OMA" id="VYIVYQW"/>
    <dbReference type="OrthoDB" id="3631276at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021988">
        <property type="entry name" value="BMT1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12141">
        <property type="entry name" value="BMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="543"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="555"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="576" mass="66742" checksum="A30809C559548576" modified="2011-11-16" version="1">MKSLVEKYVPQTFTFKVSEQEHGKTCQYINGTGILHCDDPSGLSNTTVYGDYHKHPLSLYSSLHQFTQCEETNTLYSLNISKPRLFIEDFNKMAAVLVDQLKNDDAFKDLSPFFQGKIPRMIRTDTVSKHFFKFAATSVWLEEHRVHFMVSRVLFSRTGVKWVPQLSLLYAETFNQNWEPVDFELKVQGETMKFPSFLPIPFYHNVKHMKKRWYGPEDARILLVKNEDGKDEPLVIFNAHHRRFNESYIEQHGPIPFIHYRSMFIAWPFRFELGKKATDSMPDQKYNTTRFNKARELRIEGKERQIVEKNWTPFIDTSERTIHDSHLHIVYDWEYLQVLKCQLTNLTSDSMSYCKVIFTTPGQGDVGPIRGGTELIPLSQVSTSTAPVWVGFLRTHILKCGCGREMYRPHLVVFRRHGDNFKVSHLSSSLGLDVPIQSARYMSRLCTGGDPNILMPNGISTWVYDKNTDTDYLTLSMSTGDVDNLLIDLKNIGKLVETQLEIGQGWSNDAESSLNMNACVLESSVEFCWNYGDEIKKEFLRKVQKQERKKRPAKQKQQQQQQKQKQQSSKQPDVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUQ4</accession>
    <name>G3AUQ4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor RBF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63443</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377581.1">
        <property type="nucleotide sequence ID" value="XM_007377519.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUQ4"/>
    <dbReference type="STRING" id="619300.G3AUQ4"/>
    <dbReference type="GeneID" id="18874753"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63443"/>
    <dbReference type="eggNOG" id="ENOG502QQV3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033822_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUQ4"/>
    <dbReference type="OMA" id="NVQAHDH"/>
    <dbReference type="OrthoDB" id="4094480at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="477" mass="53714" checksum="5B9D5E0978F9317C" modified="2011-11-16" version="1">MVTSNERSTVGANGHLSHEDAIKGEFDVQPVQDAVGYATSLTGDDQQYHGHQPQQQQQQQQQQQQQQQQQLQHHHHTNAYAASAAAAAAELQHRAELQRRQQQLQQQELHHQQQQLQQYHQQQQREQQQQVAQQQAVQQQQQQQLHQQQLQHQQLQQQQQQQQQQQIQVQSSRQTRHHQQQPSQHIQQQPSDDIHVGDLRSQYEDNAVINTFSSKQELVKYVKYVLGPEENCKIVINSSKPKAVYFQCERSGTFRTTVKDISKRQRVAYTKRNKCGYRLVANLYPPEKDKKKKAGDDGLDGETNEMWILRMINPQHNHLPDPINNKQKKKSKFSKTLVEKPLHKHNVPAQSATGVAAPGGGPGVGAGATNFNELIGQPVHNAGLQHIPTAASIPQHHLDPHHHHHHQHGGPSVQDAAVLAAFEASQATHSSSNVAAAAVAAALHSHNVVDSNIDPNVDPNVQAHDHSHGHRGMHVNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUS2</accession>
    <name>G3AUS2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">1,4-alpha-glucan-branching enzyme</fullName>
            <ecNumber evidence="4">2.4.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glycogen-branching enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_63457</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Glycogen-branching enzyme participates in the glycogen biosynthetic process along with glycogenin and glycogen synthase. Generates alpha-1,6-glucosidic branches from alpha-1,4-linked glucose chains, to increase solubility of the glycogen polymer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.</text>
            <dbReference type="EC" id="2.4.1.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.18" evidence="4"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377599.1">
        <property type="nucleotide sequence ID" value="XM_007377537.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUS2"/>
    <dbReference type="FunCoup" id="G3AUS2">
        <property type="interactions" value="448"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUS2"/>
    <dbReference type="GeneID" id="18874763"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63457"/>
    <dbReference type="eggNOG" id="KOG0470">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011131_2_2_1"/>
    <dbReference type="InParanoid" id="G3AUS2"/>
    <dbReference type="OMA" id="YEMHLGS"/>
    <dbReference type="OrthoDB" id="196493at2759"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11321">
        <property type="entry name" value="AmyAc_bac_euk_BE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02854">
        <property type="entry name" value="E_set_GBE_euk_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000001">
        <property type="entry name" value="1,4-alpha-glucan branching enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000003">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037439">
        <property type="entry name" value="Branching_enzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651:SF3">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000463">
        <property type="entry name" value="GlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="12">
        <location>
            <begin position="176"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="343"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000463-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW30628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="698" mass="80525" checksum="FF303174AF9FBBDB" modified="2011-11-16" version="1">MASNDSDRALIKGALDLDPWLEPFSQGLIYRQKEFRRWYKDLQNSEGSLLKFADSYKTYGIHGNHQTKEIVITQYIPEVEEVSLVGDFNNWNTETHKLNKVNNFGLWSLTIPPVNGQFAIAHDSRYKIAMKLSSGEHIYRLDPWAHRATPNDTHTVYEGRFWNPETEYQFKNKRPQIHSPQGIRVYEAHVGISTPEPKIGSYKNFTQNILPKIHELGYNTIQLMAVMEHAYYASFGYQITSFFAISSRYGSPEDLKELIDTAHGLGIRVLLDVVHSHASKNVEDGLNNFNGTDHYLFHGGARGNHDLWDSRLFNYTNHETLRFLLSNLKFFIDVYQFDGFRFDGVTSMLYKHHGLSFGFSGDYNEYFNPDWVDVEAIVYLMLAHQMMDEISSQQSIEFTTIAEDVSGMPTLCLPISAGGVGFNYRLSMAIPDMWIKILKHLSDDQWDLHNIVHTLTNRRHGEKCIAYAESHDQALVGDKTIAFWLMDKEMYTNMSTLTELTPIVDRGLSLHKMIRLLTFGLGGEGYLNFEGNEFGHPEWLDFPRVGNGESYHYARRQFNLIDDDLLRYKFLFRFDGAMQHLDEKYGVLDTPQAYVSLKHEGDKVVVFERNGLLFIFNFHPTQSFPDYKVGVEAAGTYQIVLNSDAEEFGGHSRIQELDANGKPQQYFTNKEYWNHRANSLFVYIPCRTAIVLQLQQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV54</accession>
    <name>G3AV54_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Scavenger mRNA decapping enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_144541</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HIT family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377894.1">
        <property type="nucleotide sequence ID" value="XM_007377832.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV54"/>
    <dbReference type="FunCoup" id="G3AV54">
        <property type="interactions" value="931"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV54"/>
    <dbReference type="GeneID" id="18870639"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144541"/>
    <dbReference type="eggNOG" id="KOG3969">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041045_1_0_1"/>
    <dbReference type="InParanoid" id="G3AV54"/>
    <dbReference type="OMA" id="HVHINPI"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140932">
        <property type="term" value="F:5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA] diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000015">
        <property type="entry name" value="m7GpppX diphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.40">
        <property type="entry name" value="Scavenger mRNA decapping enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008594">
        <property type="entry name" value="DcpS/DCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011145">
        <property type="entry name" value="Scavenger_mRNA_decap_enz_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12978">
        <property type="entry name" value="HISTIDINE TRIAD HIT PROTEIN MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12978:SF0">
        <property type="entry name" value="M7GPPPX DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05652">
        <property type="entry name" value="DcpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11969">
        <property type="entry name" value="DcpS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028973">
        <property type="entry name" value="Scavenger_mRNA_decap_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102860">
        <property type="entry name" value="mRNA decapping enzyme DcpS N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR028973-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR028973-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="34801" checksum="F0A5F4F7EF23C22B" modified="2011-11-16" version="1">MSISELIGKFQFVRILKSDSQTKTISLLGSIDNEHAIITLEKSHFQFENHKVNELIQDISLINNNDIYYWSIGSLLQQLPELPGAKINLIYPATETHIHKYDEQTYHYIRETPAMYEKYVVPYIKSMKGDRIKWVYNILFEGKESETFVYHDKDPSNGFVLLPDMKWDTINLQTLYLCCIVNRLDISSVRDLNENHIEFLESLQEKVKQITVEKYNIGKNQLRIFIHYQPSYYHFHIHIVNVAHPGLGDGIAVGKAILLDDVIDNLKLDGDYYKKKTIGYLLGENHGLWKIEGYTVSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV72</accession>
    <name>G3AV72_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_63495</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377641.1">
        <property type="nucleotide sequence ID" value="XM_007377579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV72"/>
    <dbReference type="GeneID" id="18874781"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63495"/>
    <dbReference type="HOGENOM" id="CLU_1464707_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV72"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442589" evidence="2">
        <location>
            <begin position="17"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="144"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW29875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="185" mass="20498" checksum="D95A07725C9EAD5C" modified="2011-11-16" version="1" fragment="single">MIGYLALFFIVTLVFGEETSYETDYKTVSTTVIFPNYHRYFNTSSVWTNSSSTSTETHTNTETHIYTTTTTTAAAANTYSEITHITSEASTHSTIEEAITHSEITTAIPANPTSFPSPICNAFTTTIYAPVSIKTVTETTTITTGEPIKSSSSISSQTKQTTEHKKSEDKVETRTDYYTYTYTYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV99</accession>
    <name>G3AV99_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Histidinol-phosphatase</fullName>
            <shortName evidence="8">HolPase</shortName>
            <ecNumber evidence="3 8">3.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_52755</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>L-histidinol phosphate + H2O = L-histidinol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14465"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="3.1.3.15"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 8/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the PHP hydrolase family. HisK subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.15" evidence="3 8"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377668.1">
        <property type="nucleotide sequence ID" value="XM_007377606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV99"/>
    <dbReference type="FunCoup" id="G3AV99">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV99"/>
    <dbReference type="GeneID" id="18871719"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52755"/>
    <dbReference type="eggNOG" id="ENOG502RXUQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054611_0_1_1"/>
    <dbReference type="InParanoid" id="G3AV99"/>
    <dbReference type="OMA" id="DYDRPMY"/>
    <dbReference type="OrthoDB" id="5957391at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00013"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004401">
        <property type="term" value="F:histidinol-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010140">
        <property type="entry name" value="Histidinol_P_phosphatase_HisJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01856">
        <property type="entry name" value="hisJ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21039">
        <property type="entry name" value="HISTIDINOL PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21039:SF0">
        <property type="entry name" value="HISTIDINOL-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHP" evidence="9">
        <location>
            <begin position="25"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW29902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="280" mass="32471" checksum="35D532EEF63E5045" modified="2011-11-16" version="1">MPRLESRFLYPEETDRDYSIDNLVQDFDKYVNHVVQLQQRYNDKDGMKIILGFEVEGIDDPHIEYSQALLARNPSIQMMVGSVHFVHQIPIDFTPELWQRAREATTEKTTRALFRDYFALQYRVISGLKPMVVGHFDLIRLLLPADDIDTTTGKLTSEVNIEAEWPEVWDLIVQNIDCVKQYGGLFELNSSALRKGWDTSYPKRDIADAIVKYGDARFCLSDDAHSLTQIGIHYDKTWKYVKSLGVDKIYCLELTDENRVKAVTKDIASLDKSSFWKQYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVF2</accession>
    <name>G3AVF2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Short-chain dehydrogenase/reductase 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Retinal short-chain dehydrogenase/reductase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_73554</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the reduction of all-trans-retinal to all-trans-retinol in the presence of NADPH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377937.1">
        <property type="nucleotide sequence ID" value="XM_007377875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVF2"/>
    <dbReference type="FunCoup" id="G3AVF2">
        <property type="interactions" value="518"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVF2"/>
    <dbReference type="GeneID" id="18875490"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_73554"/>
    <dbReference type="eggNOG" id="KOG1201">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_5_1_1"/>
    <dbReference type="InParanoid" id="G3AVF2"/>
    <dbReference type="OMA" id="NWYAVLP"/>
    <dbReference type="OrthoDB" id="10253736at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052650">
        <property type="term" value="F:all-trans-retinol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05339">
        <property type="entry name" value="17beta-HSDXI-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000131">
        <property type="entry name" value="Short-chain dehydrogenase/reductase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322">
        <property type="entry name" value="PKSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322:SF736">
        <property type="entry name" value="RETINOL DEHYDROGENASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW30171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="339" mass="36837" checksum="82EB03CE119E1F71" modified="2011-11-16" version="1">MSITTLYWLNHKFKILSDLIIGNTFEPKFDIVCVTGGSTGLGKEIVKQIADRGARVVVLDLIVPDDQDKIPNATYYKCDVSDRQQILDSQKQISQQIGTVTVLINNAGITTGRTVLDLSFQEIEKTIQINLLSSFYTIKAFLPDMLNLKRGYIVTIASVLGYMSPARLSAYGASKSGLIALHESLTYELGPPSVNPHGVKTLLICPGQLRTNMFQGVATPSKVLAPELEPEFVASCVVSAIEQGRRGEIKLPLYGKFIPLFRAMPWPIVELTRKISGIDKSMNHFKDKLSRVASSVASIASRSKNPSLLGTPAAVGPSKNLLARASSSAVAQKHSAHTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVM6</accession>
    <name>G3AVM6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_63804</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377957.1">
        <property type="nucleotide sequence ID" value="XM_007377895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVM6"/>
    <dbReference type="GeneID" id="18874922"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63804"/>
    <dbReference type="HOGENOM" id="CLU_3070179_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVM6"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="53" mass="6293" checksum="07394FE72C3EBB37" modified="2011-11-16" version="1">MTHLPIHPSKARIHTRIKFQKYKSSSSENASYYIYTEADPETVASDYLNYEFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AED6</accession>
    <name>G3AED6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58922</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373136.1">
        <property type="nucleotide sequence ID" value="XM_007373074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AED6"/>
    <dbReference type="STRING" id="619300.G3AED6"/>
    <dbReference type="GeneID" id="18872680"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58922"/>
    <dbReference type="eggNOG" id="ENOG502SE22">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156033_0_0_1"/>
    <dbReference type="InParanoid" id="G3AED6"/>
    <dbReference type="OMA" id="QGFLWTG"/>
    <dbReference type="OrthoDB" id="2430343at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140580">
        <property type="term" value="F:mitochondrion autophagosome adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013898">
        <property type="entry name" value="Atg43"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38699">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38699:SF1">
        <property type="entry name" value="MITOPHAGY RECEPTOR ATG43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="141" mass="15695" checksum="6177E97F366F10CB" modified="2011-11-16" version="1">MPPHIAIPDLRFEQSFLKQLHKYAGDVQAPPPVSPNKLKRKHNAPVLTDAELQLMNHNLDVQEQQQLQPLPISTGIVVYAVIKDQIIMPLLQGFLLSYALLSIRPVLRLVVKNGQEVGGWVSRLIGLNRLGAGRVSVVKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEG7</accession>
    <name>G3AEG7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="13">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_58959</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="13">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="13"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373167.1">
        <property type="nucleotide sequence ID" value="XM_007373105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEG7"/>
    <dbReference type="FunCoup" id="G3AEG7">
        <property type="interactions" value="669"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEG7"/>
    <dbReference type="GeneID" id="18872695"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58959"/>
    <dbReference type="eggNOG" id="KOG0660">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="InParanoid" id="G3AEG7"/>
    <dbReference type="OMA" id="CYFLYQM"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07849">
        <property type="entry name" value="STKc_ERK1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000040">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000073">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="6 13 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="4 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="23"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW35755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="359" mass="42024" checksum="6062334FBE55406D" modified="2011-11-16" version="1">MNVNQQVQQSQPRQVSFNVSDHYQIIEIVGEGAYGIVCSAIHKPSNQKVAIKKIEPFERSMLCLRTLRELKLLKHFNHENIISILAIQRPASYETFNEIYLIQELMETDLHRVIRTQKLTDDHIQYFIYQTLRALKAMHSANVLHRDLKPSNLLLNSNCDLKVCDFGLARSIASSEDNFGFMTEYVATRWYRAPEIMLTFQEYTTAIDVWSVGCILAEMLTGKPLFPGRDYHNQLWLIMEVLGTPNMEDYYNIKSKRAREYIRSLPFCKKVPFQVLFNNPDINPLALDLLENLLIFNPAKRITVDDALKHPYLKLYHDPNDEPISEKIPEDFFDFDKRKDELTVEELKKMLYDEIMKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AET1</accession>
    <name>G3AET1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-splicing factor SYF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_132070</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the crooked-neck family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373173.1">
        <property type="nucleotide sequence ID" value="XM_007373111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AET1"/>
    <dbReference type="FunCoup" id="G3AET1">
        <property type="interactions" value="1069"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AET1"/>
    <dbReference type="GeneID" id="18869633"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132070"/>
    <dbReference type="eggNOG" id="KOG2047">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007736_3_0_1"/>
    <dbReference type="InParanoid" id="G3AET1"/>
    <dbReference type="OMA" id="IWYNYLR"/>
    <dbReference type="OrthoDB" id="10067343at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000349">
        <property type="term" value="P:generation of catalytic spliceosome for first transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055433">
        <property type="entry name" value="HAT_Syf1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056350">
        <property type="entry name" value="HAT_Syf1_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055430">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF5">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SYF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23231">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23233">
        <property type="entry name" value="HAT_Syf1_CNRKL1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23220">
        <property type="entry name" value="HAT_Syf1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats" evidence="12">
        <location>
            <begin position="13"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor SYF1 central HAT repeats" evidence="10">
        <location>
            <begin position="187"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1/CRNKL1-like C-terminal HAT-repeats" evidence="11">
        <location>
            <begin position="390"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="772"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="776"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW35761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="791" mass="93371" checksum="6DE7F19EA55527BD" modified="2011-11-16" version="1">MIFKLEDLIQEGDIPYEESIAKDPTNITNWTTYLEFKSRSSFHNKAFILHRATSAIADSEELWQLYLDLLIEHIHDNLSVLTDRQYEHLNSVFNKAVQCCSASVPLWKCYLEMLLETQIPKVTYIRRTFNGCLRSLPLKAHGQVWPLYLSFADRIGGMTAVKIYTKYVQYLDPSALKGTKEGGTSFDDIISKLTEFGEVDKTVGIYEQILSHPDEYTTLGKSPVQYLFEYIDLLLHFPTEPLFFDKIISDSIKSYPDQIGKLYLKSVEFFKQKHDVEKVRYYYDNGIKTCLTSQDFVALYDEYTEFEESELAKVSKEDPTLVNFKLDKFEKLLDDREILFNDMKLRQNIHNLDVWFDRFDIYKNNLGKLLQTYTDALKSINPLKVVSTNHKLCEIWIQYASIYSNNKDYKTADFIYSKSITSQFLHPDELAEIYIQWCEMVLGTDQFPETYAIEILDSVMYREYDEDFQYTDSTITVQKRIVKSRKLWNFYIDLLESFVESPEQVEDIDKVGKAYEVLISKKIITVKDILAYANFLHTWKYHERAWQIYERGLQLFVDPELKFEIWNVYLPDMIKHQGDTERIQDLFEVSLKQVPAWLMNPILVLYSQYEKDHSRIINSINILIKSLKTFDKAQREHTNRLTEITTMKFEIVKIILIKLIEIKDINQFRQIAQDAIEDDYFALQQFIQLAQIFIDFEITQSEFNRVRELYKYVCGLSTSDKVKVMWESWENFELEYGNETSFKDMLRYKRNVSAPITAKVEVSNPMGFVKATEEKKDEEEVENPDEIDLDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEW9</accession>
    <name>G3AEW9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_59006</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373211.1">
        <property type="nucleotide sequence ID" value="XM_007373149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEW9"/>
    <dbReference type="FunCoup" id="G3AEW9">
        <property type="interactions" value="536"/>
    </dbReference>
    <dbReference type="GeneID" id="18872717"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59006"/>
    <dbReference type="eggNOG" id="KOG3662">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011607_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEW9"/>
    <dbReference type="OMA" id="LHCMKYP"/>
    <dbReference type="OrthoDB" id="5977743at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033308">
        <property type="entry name" value="PGAP5/Cdc1/Ted1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315">
        <property type="entry name" value="METALLO PHOSPHOESTERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315:SF4">
        <property type="entry name" value="METALLOPHOSPHOESTERASE, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="6">
        <location>
            <begin position="82"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="425" mass="49747" checksum="29B27E811BDE1B87" modified="2011-11-16" version="1">MEVRKRHVGEESSPSKVANVNANEKANGLKYKYSIQWHLILSFSLIWISLIHYYERIVPYSTLEKCLWSNWEDWPMDRHPHRIALIADPQIIDDYSYPKQFKVLNFIVKQISDNYLHRNFQVMQQVLDPDTTIFLGDLFDGGRYWDDAKWIPEFHRFNRVFPARTDRRDIRSIPGNHDIGFQTIKRDVVDRFAKYHGKSNDYITLGNHTVIMFDSISYSHADPEISKDAKQFIDTINQEISHDLPRILLTHVPLYRFKEIQLCGPHRESKKLFPIMYGDEYQTVIAWEFSQNMLKTFQPEIIFAGDDHDYCDIIQEYEISEGIVKQTREIAVKSAAMTSGIKHPAIQLLSLSNPEGSTGITYKTEMCYMPAPYAGIYSYILFLLLSNSYFVYRLKSRGGRQEIFKHTLLNLIIGSVILKTYFLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFG2</accession>
    <name>G3AFG2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_69321</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. RarA/MGS1/WRNIP1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372363.1">
        <property type="nucleotide sequence ID" value="XM_007372301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFG2"/>
    <dbReference type="FunCoup" id="G3AFG2">
        <property type="interactions" value="841"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFG2"/>
    <dbReference type="GeneID" id="18875264"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_69321"/>
    <dbReference type="eggNOG" id="KOG2028">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017985_0_1_1"/>
    <dbReference type="InParanoid" id="G3AFG2"/>
    <dbReference type="OMA" id="HNFECSY"/>
    <dbReference type="OrthoDB" id="10265467at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000731">
        <property type="term" value="P:DNA synthesis involved in DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18139">
        <property type="entry name" value="HLD_clamp_RarA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3710.10:FF:000005">
        <property type="entry name" value="AAA family ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000001">
        <property type="entry name" value="Putative AAA family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000137">
        <property type="entry name" value="Replication-associated recombination protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3710.10">
        <property type="entry name" value="DNA polymerase III clamp loader subunits, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032423">
        <property type="entry name" value="AAA_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051314">
        <property type="entry name" value="AAA_ATPase_RarA/MGS1/WRNIP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021886">
        <property type="entry name" value="MgsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13779:SF7">
        <property type="entry name" value="ATPASE WRNIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13779">
        <property type="entry name" value="WERNER HELICASE-INTERACTING PROTEIN 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16193">
        <property type="entry name" value="AAA_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12002">
        <property type="entry name" value="MgsA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="196"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="631"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="637"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="659"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="703"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="715" mass="80455" checksum="EC059571BA6FCE9D" modified="2011-11-16" version="1">MEESCPICGKAIDQKLLERHVNSCLDNLEGTSATRADSVGTDVDDSNDDIHIVGTRKRDAFSALGLKADSSQKKKKGKSDVKGPTLTSLLLQERRVKQELRQSPQEKPKEPPLEEQLKEMAAAVNASQIKKEEIVEEPPKRQSDILAKANEMHELKRLAGLPLAHRLRPKSLDDFYGQEHLLGKNGPLRNIIEADHIPSFLLWGGPGIGKTTLARIVAKSTSCKFVELSGADSNAKALKQVFTHAENHRNLTGQRTILFLDEIHRFNKAVQDLLLPAIEKGIVTVIGATTENPSFTLNNALLSRVHTFVMEPLTSESIVKILGRALYEVNKLRKQLYGMHYISLSKDAFDYIAGLSMGDSRVALNILESVNAYLSTSKFSTDNEEAQKDIPESQNKQGVIKITESILRPLLKTRNFHQVYDRKGESHYDIISAFHKSVRGSDPDAAMFYLVKMLSGGEDPLFIIRRMIVIASEDVGLRDSSCLPFVVAAKDALEFVGMPEGEIILAHVANKLARAPKSTKSYRSLRTAQKLISEEPELTKLPVPLHLRNAPTKLMKELGYGQEYKYNPSFAYGKVKQGYFPEGMEPVKFLEDVHLGHAQDPQVSEKEYESSRLAVEDYEKFKRSYREKLRQQYKNGTKKEKNGEQTQYKKEPSFGYDEFLDKDAQPEYFEGEGHSYSDDDPECTNNFGKSYDEFLDPDSQPDYFDDDTEADLDQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFJ3</accession>
    <name>G3AFJ3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Globin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_64183</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372394.1">
        <property type="nucleotide sequence ID" value="XM_007372332.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFJ3"/>
    <dbReference type="GeneID" id="18874962"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64183"/>
    <dbReference type="eggNOG" id="KOG3378">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_821751_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFJ3"/>
    <dbReference type="OrthoDB" id="436496at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008941">
        <property type="term" value="F:nitric oxide dioxygenase NAD(P)H activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071500">
        <property type="term" value="P:cellular response to nitrosative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046210">
        <property type="term" value="P:nitric oxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01040">
        <property type="entry name" value="Mb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044399">
        <property type="entry name" value="Mb-like_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396:SF6">
        <property type="entry name" value="ABL201WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396">
        <property type="entry name" value="FLAVOHEMOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Globin" evidence="1">
        <location>
            <begin position="96"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="338" mass="38477" checksum="A95403828331985F" modified="2011-11-16" version="1">MHSGSEGFSYSFSYVIIEHLTVLGACGFKEENSTENFSSLWFQENQHFVLGPILIRYTWNTLLVEDEGTTLKRQTRPSSPDFLCECTEDITPRSSEDSFDSQDTITGMARVIKSNSTGIATSLFCRQFYANIIARDREMKNIFPSIEHQSLDLASVLTIVVLQLDDLSSLEDYLMKLGKRHTRILNIEPVHYEILGEVLIQTFHERFGNKFNQELEIVWIKLYLFLANSLLQYGIDPVLNVEKEAVDLKRVPTHMSTSSVRTEKTLVGSPKKGLYQDEKAENSDIDSVTNEVHMIAYICANKASFCKLAFRYDNLTNRNITMFTAGIVLATESYLVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFV3</accession>
    <name>G3AFV3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">26S proteasome regulatory subunit 7 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_58235</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007372441.1"/>
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        <property type="interactions" value="1118"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFV3"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_000688_6_0_1"/>
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    <dbReference type="OMA" id="RSKYHIE"/>
    <dbReference type="OrthoDB" id="1937997at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:ubiquitin protein ligase binding"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of protein catabolic process"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of RNA polymerase II transcription preinitiation complex assembly"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="26S protease regulatory subunit 7"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="26S protease regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
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        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="223"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00067449"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW35091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="448" mass="50099" checksum="87DCE3B5E3F4432A" modified="2011-11-16" version="1">MPPKADWDKYIAPTEDDEKNQEKIVPLSEGDIQVLKTYGAAPYATSLKEIEKDLKDIEERIKENIGVKESDTGLAPPHLWDVVGDKQRMQEEQSLQVARCTKIIEATQTSASTTPGLQNADTKSKYVINIKQIAKFVVGLGERVSPTDIEEGMRVGVDRQKYEIQLPLPPRIDPTVTMMTVEEKPDVTYSDVGGCKDQIEKLREVVELPLLSPERFVKLGIDPPKGILLYGPPGTGKTLCARAVANRTDATFIRVIGSELVQKYVGEGARMVRELFEMARTKKACIIFFDEIDAIGGVRFDDGAGGDNEVQRTMLELITQLDGFDPRGNIKVMFATNRPNTLDPALLRPGRIDRKVEFSLPDLEGRANIFRIHSKTMSCEKDIRWELISRLCPNATGAELRSVCTEAGMFAIRARRKVANEKDFLKAVDKVIKGNLKFSSTSEYMQYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFV5</accession>
    <name>G3AFV5_SPAPN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58236</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Saccharomycotina</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="18872376"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58236"/>
    <dbReference type="HOGENOM" id="CLU_3070171_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFV5"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="53" mass="6115" checksum="5C4340E4B57DB540" modified="2011-11-16" version="1">MALLGYEFGILPKAKNSRQWSRPLAVRPHKLGSCLFYRYEAIICALKYLEALD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG97</accession>
    <name>G3AG97_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_132123</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
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                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG97"/>
    <dbReference type="FunCoup" id="G3AG97">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG97"/>
    <dbReference type="GeneID" id="18869637"/>
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    <dbReference type="eggNOG" id="ENOG502QT7N">
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    </dbReference>
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    <dbReference type="InParanoid" id="G3AG97"/>
    <dbReference type="OMA" id="INWIGKV"/>
    <dbReference type="OrthoDB" id="3980110at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="859"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="877"/>
            <end position="888"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="888" mass="102305" checksum="5142C54EEF364A2F" modified="2011-11-16" version="1">MSDNESGEEIDKTVAEFDFALPINRITKDLTKRHDVLAILLDLEEDPELDFIFKTIASLCDPFTQPQESEDTILSVDFISDLVISIVYSHYVEENEQDIKENNVDIQSIKFDISTQLFTIVRRIHQYLNAEDELELRFVNNDEEHYKENLPHWTPNLSISNDEANAKLVYSIACVLLLSIYKLFVPKTGEYNVAMNPYLHYFLKLWKCHTNIILLGLEIDRRIEFQNHERGTEEETPEIVKLTLKGSSSIRYVLAWIINQNPSSTFETDSISPFDKEDLQKVTVFNFIEPLAKKKTNGGALSIDMRLVIIALLIINSGTSFTAAQYQLSERPSRNEEEASRRLNQSRKITELGDILIDLEYDDLFDEDIKYIFEYEYDGSDGEISDHEEDKPSLHEDIISDEDIPGKIVEDGIEFDEYGRDWRDMARGDNLIYQDSFSSMFDEFDKLEDKEQSDDIFASWEELYNTFEFLNTNSIDGNSEAEKRVGQVVINTIAKAVRDKLTKKELPPITPEMIYSYWSSSATEKEFEITQNNNKVIVPIFNITKFEIFLHNNSKLARCAMDEMLMYNGSRRIFIWFITHNLNISNLLIDYVFQLLAGLRGNSKTQGPYVFSRKGGKVILSEVEQSMLLHEFLTNSNAYFSAAEGIEISDGYKVVLAESIAKKQIQVICLMIQQLINIGVIDLSLVHNTDDDKDDIHDYRNEIQILLINWIGKVPEARQLFFKVKNTDGEEEPKHVPKSTITLEEENKLFERYSNMSTTEISNVIDKKNVEILDNFTSRIESHLSSLLSVQGNGNSNLKQLTDDFRFFLANFNTLCKIEYVAETLFDKFEPVLLTGMITNVGNPKANNTFDAEFNNEFLNGEGKFQSPPEGEPESKKKNKKKKKSRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGB5</accession>
    <name>G3AGB5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Ubiquitin-related modifier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">URM1</name>
        <name evidence="7" type="ORF">SPAPADRAFT_48272</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by the MOCS3 homolog UBA4. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as AHP1. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 6">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="5">C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of UBA4, then thiocarboxylated (-COSH) via the rhodanese domain of UBA4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the URM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372667.1">
        <property type="nucleotide sequence ID" value="XM_007372605.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGB5"/>
    <dbReference type="FunCoup" id="G3AGB5">
        <property type="interactions" value="902"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGB5"/>
    <dbReference type="GeneID" id="18871202"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48272"/>
    <dbReference type="eggNOG" id="KOG4146">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148208_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGB5"/>
    <dbReference type="OMA" id="IHFMAEK"/>
    <dbReference type="OrthoDB" id="72325at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097163">
        <property type="term" value="F:sulfur carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007114">
        <property type="term" value="P:cell budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01764">
        <property type="entry name" value="Ubl_Urm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03048">
        <property type="entry name" value="Urm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015221">
        <property type="entry name" value="Urm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14986">
        <property type="entry name" value="RURM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09138">
        <property type="entry name" value="Urm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037379">
        <property type="entry name" value="Ubiquitin-related_modifier_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="modified residue" description="1-thioglycine" evidence="5">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)" evidence="5">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="101" mass="11267" checksum="0F4A99883E565935" modified="2011-11-16" version="1">MTIKVKVEFLGGLDVISNSIREHKCVVPYEEGEATMKELIDHITKTIISDPKDIPVFIEDGTVRPGILVLINDTDWELEGMEEYVIESGDVFTFTSTLHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGC3</accession>
    <name>G3AGC3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">6-phosphofructo-2-kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_58473</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372674.1">
        <property type="nucleotide sequence ID" value="XM_007372612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGC3"/>
    <dbReference type="FunCoup" id="G3AGC3">
        <property type="interactions" value="74"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGC3"/>
    <dbReference type="GeneID" id="18872465"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58473"/>
    <dbReference type="eggNOG" id="KOG0234">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041936_1_0_1"/>
    <dbReference type="InParanoid" id="G3AGC3"/>
    <dbReference type="OMA" id="SSHLIQC"/>
    <dbReference type="OrthoDB" id="267323at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF32">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="3">
        <location>
            <begin position="122"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="363" mass="41507" checksum="56423D5CF718E367" modified="2011-11-16" version="1">MSTYTNSNTKYKDIPQPKNVTFDLFSPNYSPFESTFMSEHKHMIDSDLSPTDYSGASPVDIRVTNSETSINSLFDAPNTLPSTTTITPVVSKTDFKPLTEFEFSSIINTKLNIQQQLQKNIPQTDEKLVVILVGLPASGKSTICTQLKNFINKSTIYQAQIFNAGDVRRRNSCFNDAAFFDPSNEQGKKDRELYATITVSNLINSLNNNAIQVGFLDATNTTVDRRKRMIDTVMKEAKGNVKVIIFDVQCNDAKYLNFNINGKAGNNDYRGTDYSAAIADFKKRTEHYIKMYQPVTQEELQNLPLALYMKVVNAGETFDLANVEPEFQADSYWYKILNEFKEHYYENEGRRYKQAVDRWYNQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGH2</accession>
    <name>G3AGH2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58533</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372723.1">
        <property type="nucleotide sequence ID" value="XM_007372661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGH2"/>
    <dbReference type="GeneID" id="18872492"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58533"/>
    <dbReference type="HOGENOM" id="CLU_719944_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGH2"/>
    <dbReference type="OrthoDB" id="10507934at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="384" mass="43420" checksum="1E9616758CDC486F" modified="2011-11-16" version="1">MTDVSDHRVSRMFRLKHGLSSTDQISQYSEESEPQPSTYQRSPPRRTGSLRLFRKSSGLSTNGDRSSKIISNIFNRSKSSVERESMYLQPNDSKTWVTGFQLNTPFIQPQLPVTLESFADSDDETSDDEVDINSILLDVKTTNYISLFQESFDDGNYTTIYKTTSKISGDPLLHTLSSIEERSQSKPQLSIDTTLQKQPQQVPDMESYSMGMSAPSSNFYSISPQTFDSQDNYPEPSIRLEDKDLSPCEETTREYFKMKQLPSPHRAENIDKLKNSKVIPEVAQYKPQFASPPVPSCTSVSYSDKTVISDPISKSATFERSTPDSSQEDITLKKKENHDLKSSSIMLVAKEISDERQSKSKIIKWFSKVLKTKQSKGHTAIIAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGH3</accession>
    <name>G3AGH3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58534</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372724.1">
        <property type="nucleotide sequence ID" value="XM_007372662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGH3"/>
    <dbReference type="GeneID" id="18872493"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58534"/>
    <dbReference type="HOGENOM" id="CLU_3051912_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGH3"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="54" mass="6299" checksum="CA9DD9E6A382B3B7" modified="2011-11-16" version="1">MAIHELSTNVHDRTCALKPIIYMIMKISSFLYHMKLSSCSSRLSNYTIFRPTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGJ3</accession>
    <name>G3AGJ3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Pheromone a factor receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_146456</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372744.1">
        <property type="nucleotide sequence ID" value="XM_007372682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGJ3"/>
    <dbReference type="FunCoup" id="G3AGJ3">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGJ3"/>
    <dbReference type="GeneID" id="18870717"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_146456"/>
    <dbReference type="eggNOG" id="ENOG502S44N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027592_4_0_1"/>
    <dbReference type="InParanoid" id="G3AGJ3"/>
    <dbReference type="OMA" id="ATTEICC"/>
    <dbReference type="OrthoDB" id="2874149at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004932">
        <property type="term" value="F:mating-type factor pheromone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14966">
        <property type="entry name" value="7tmD_STE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001499">
        <property type="entry name" value="GPCR_STE3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28097">
        <property type="entry name" value="PHEROMONE A FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28097:SF1">
        <property type="entry name" value="PHEROMONE A FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02076">
        <property type="entry name" value="STE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00899">
        <property type="entry name" value="GPCRSTE3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0589">Pheromone response</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="119"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="360"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW35332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="421" mass="48092" checksum="8CE2A8DEB4E4C291" modified="2011-11-16" version="1">MDSRAAGLAAFALISWILLIPPFIWHCKCKNFPAICLVFWLSFVNFNVFFNTMVWGHDNFYHAWDGKIWCDVTVRLEAGSSTGKICAITSLILNLYMILRAKPGVFLEFNSWKKRAIDLSICLITPIFIMSTNYIISAFRYGVFKYEGCVSMFGPNYATIGLDHMWPIIWSSIALVIAIMTLYSFFTQRKEISNILVCTNSGLTYQRFARLLIFCLLIILAMAPLSGYYFADIFKYKLHKFEWDEVHQEDWGEIMYYDVGFTVGYRRLVNSILSIIAFLLFGLGSDAVEMYKSIINDTILLFSRKVPGEEKDQVTFLTTLPNRATRIFSNKSDKTPSQNSAGTTLNEYGQFNNAMSDMDVKHTSTSQSNKSKESGPPLPNGTFFSNVDNYLEQELKSIIMQETETGKSGQKCSYRFQVEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGM4</accession>
    <name>G3AGM4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_48363</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372775.1">
        <property type="nucleotide sequence ID" value="XM_007372713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGM4"/>
    <dbReference type="GeneID" id="18871212"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48363"/>
    <dbReference type="HOGENOM" id="CLU_683638_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGM4"/>
    <dbReference type="OrthoDB" id="2852960at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="17"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="403" mass="47318" checksum="3E5A432935466DFB" modified="2011-11-16" version="1">MVLELQQHQYQSKSRKSPALLDLPDEVLELIINQLTQLDIVQVRLLNSKLCDLASRKLYKNIYLNNSGPRIYLSNKLHTSFYDNYTIVNDKKKFKKVIPNRNMKYTQQIMFRIEHEDEIYLKMYIVELFPHIVIKGDPFDEEYFIYSDGISHFHLPSFRKPPQDRSLVNRLTIDSPLSEEVDIISQFSNIAKVELSEIDNFTLNLLSNFSHNFCKVTSLTISDRLELDIKLLARLFKLDQITSFQLQLYRNLGDGSIYSDFKWILSQMTNLKHLVLSSNLGLENYLSGQLNPNSLLSFVLISNYGPRYEFTLPMFLLEQQSITRLCFSTSTLETSIFWNPNRFERISRTNGEQNRGDKGATNYIQNLIRFGKCPRLNQFIINNTYYFVERHSNDSIDFTKFPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGP7</accession>
    <name>G3AGP7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Chromatin assembly factor 1 subunit A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_130746</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372792.1">
        <property type="nucleotide sequence ID" value="XM_007372730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGP7"/>
    <dbReference type="FunCoup" id="G3AGP7">
        <property type="interactions" value="126"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGP7"/>
    <dbReference type="GeneID" id="18869526"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_130746"/>
    <dbReference type="eggNOG" id="KOG4363">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028547_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGP7"/>
    <dbReference type="OMA" id="TIQDIAC"/>
    <dbReference type="OrthoDB" id="79480at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033186">
        <property type="term" value="C:CAF-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048800">
        <property type="entry name" value="Cac1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022043">
        <property type="entry name" value="CAF1A_DD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15272:SF0">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15272">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT A CAF-1 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21796">
        <property type="entry name" value="Cac1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12253">
        <property type="entry name" value="CAF1A_dimeriz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromatin assembly factor 1 subunit A dimerization" evidence="6">
        <location>
            <begin position="303"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Chromatin assembly factor 1 subunit Cac1-like C-terminal" evidence="7">
        <location>
            <begin position="494"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="352"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="556" mass="64870" checksum="BCDF45E091F76338" modified="2011-11-16" version="1">MDVDLSLQPTSTDDDTSSQSSDKENLNTKQADKLAKKLEREKQKELEKKAKEEEKLAKKRQLEQDKLERKRKIEEERESKKRKLEEEREAKRRKLEEEKELKRKKLEEEKELKRKKLEEEKLLREQKRLEEKLAKEKEREEKKRKLEEEKRIKELERKRIEEEKKKAEELKERSQMKISSFFQVKPAQPKSQEISTTPIVIASSPSEIVCKYDSEFLPFFVQKNVTVMSRPRKDLSQTQKELNDIIANPSQEQFTSFLKSLPMTASTSATHQTTPDEIITALNSSTTTESQVYQLINQLPPIKYISFYENEKPPYIGTWCSTKHKSQQASIIANPLNSELTGFDYDYDSDLEWNKDDEDGEDLDDDVDDDEDDSMLIPDEEDDEFVENDGIRKKKFISLSIINKWNNDENKSFFQDFSVVVMTEGVQVPVEGPIDPFCNYWKGEEVVMAAADESNATLNEQVQAVALSTPVKNTMANPNILTPSKKTITDPIILKSLIQFLEKNNDFTIGTLVELSKKEFKQFTKALLKNTIQDIACYNKKTSIWEIKQEIKTRYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH09</accession>
    <name>G3AH09_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_58669</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Mitochondrion inner membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372851.1">
        <property type="nucleotide sequence ID" value="XM_007372789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH09"/>
    <dbReference type="FunCoup" id="G3AH09">
        <property type="interactions" value="454"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AH09"/>
    <dbReference type="GeneID" id="18872558"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58669"/>
    <dbReference type="eggNOG" id="KOG1733">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141397_0_1_1"/>
    <dbReference type="InParanoid" id="G3AH09"/>
    <dbReference type="OMA" id="MAAWNQV"/>
    <dbReference type="OrthoDB" id="7813104at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042719">
        <property type="term" value="C:mitochondrial intermembrane space chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140318">
        <property type="term" value="F:protein transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.810:FF:000001">
        <property type="entry name" value="mitochondrial import inner membrane translocase subunit TIM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="15">
        <location>
            <begin position="29"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW35439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="99" mass="11090" checksum="99BF50BFD58DCA21" modified="2011-11-16" version="1">MGLFGSSSASAPADPASAKSQQVKQEIQQQISQELATANATELVRTITENCFDKCIFKPQESLSFQEDQCIAQCREKYMRSWNVISKSYISRIQEAAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH34</accession>
    <name>G3AH34_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ferric-chelate reductase (NADPH)</fullName>
            <ecNumber evidence="3">1.16.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">SPAPADRAFT_132577</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2 a Fe(II)-siderophore + NADP(+) + H(+) = 2 a Fe(III)-siderophore + NADPH</text>
            <dbReference type="Rhea" id="RHEA:28795"/>
            <dbReference type="Rhea" id="RHEA-COMP:11342"/>
            <dbReference type="Rhea" id="RHEA-COMP:11344"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.9" evidence="3"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372876.1">
        <property type="nucleotide sequence ID" value="XM_007372814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH34"/>
    <dbReference type="FunCoup" id="G3AH34">
        <property type="interactions" value="394"/>
    </dbReference>
    <dbReference type="GeneID" id="18869671"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132577"/>
    <dbReference type="eggNOG" id="KOG0039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010365_4_0_1"/>
    <dbReference type="InParanoid" id="G3AH34"/>
    <dbReference type="OMA" id="YDGWTRH"/>
    <dbReference type="OrthoDB" id="167398at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF9">
        <property type="entry name" value="FERRIC REDUCTASE TRANSMEMBRANE COMPONENT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442499" description="ferric-chelate reductase (NADPH)" evidence="18">
        <location>
            <begin position="21"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="374"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="19">
        <location>
            <begin position="408"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="616"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EGW35464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="715" mass="82516" checksum="20C54693C549D499" modified="2011-11-16" version="1">MKLLCLLYSLFFFFVAGVSARGKFEWYGKTITLYSCVYEINYLKDFHFCPPTDRTCLCSNENSRATLAGCFAYKDRNSTYLVDYVVGYCARYFETELEANWFDKSYERFTNYARNQSEIEPTEEPIDFPLRFSSKDMDFYSEIANRFLLNYDNSIWYGVSVFGFWLLVLIVSSISYWTKVLFPTATKRLVGPVANFYRKHVSMPALYGKNKAQEMRGFKVFDGLVPTRFEMIVIALFYIFVIIIHAVNSRALKGDPVFGSTYLAELRYVADRTGIVAIFMMPLLFLFAGRNNFLQWLTGINYCTFLCYHRHIARVMFMLVAIHSVNYTILLVNRKYFGEELREPWLYWGLIATIVGGIMLIQSILYLRRNWYELFLLVHIVMAVLYVVGTWIHIDDFGYGIILYPTIAVWSFDRLVRIGRLLYFGFPEVNVKLVGDETIKMCIPKPKHWKIIPGGHAFVHFIKPTYFWQSHPFTFTNCSDQGEDKIVMYMKVKGGVTHSLYKLLNKSPGKMTTMRIGIEGPYGESTPAKYADKAVFIAGGNGIPGIYSEICDIALRSSPERKSVLKLVWVIREYSSILWFYDELVRLKNTNIETTVYVTRPDVSITNADEFTHIGSSQDGSIKSSEETKSSSSSFDGANAVGKLKEELSHVHFEEGRPDMEQLVQDEIKESPGSTAFVTCGHPIMVDEIRYHSCKNIDNEEKKRVDFFEQLQVWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH40</accession>
    <name>G3AH40_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Altered inheritance of mitochondria protein 24, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_48460</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the AIM24 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372882.1">
        <property type="nucleotide sequence ID" value="XM_007372820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH40"/>
    <dbReference type="GeneID" id="18871225"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48460"/>
    <dbReference type="eggNOG" id="ENOG502RZF6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069182_0_0_1"/>
    <dbReference type="InParanoid" id="G3AH40"/>
    <dbReference type="OMA" id="WIILQDY"/>
    <dbReference type="OrthoDB" id="5295771at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007007">
        <property type="term" value="P:inner mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.160.10">
        <property type="entry name" value="Mitochondrial biogenesis AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002838">
        <property type="entry name" value="AIM24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036983">
        <property type="entry name" value="AIM24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016031">
        <property type="entry name" value="Trp_RNA-bd_attenuator-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36959">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 24, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36959:SF2">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 24, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01987">
        <property type="entry name" value="AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51219">
        <property type="entry name" value="TRAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="293" mass="32888" checksum="43ACDF78F7C0F62E" modified="2011-11-16" version="1">MKLIKQLFNKTKVIQKPLVTGNGPFIELPKFIPIDEQLLNITLPKHSSLNIRNGSLLAINGNITDIQSVPSNSYQKLTSSGFNSLVINGNYSIVDTKDTWTILNEQDLIAYTGYNFDVTPTSILSKFRSIQTKGDGTIVLNGNLFNIELAANEEILVNPNALIATTSTVNFQVLNKAKYSKLDWALPTQSIFGSLTSPITSLLDKITQYNKDIVSKITQNNSYRNLLDYTGPAKTRLKNLVDWIVLYATTIFKRKPIYIKIKGPTKLLINNETVVSNTKVFTRKEIKTIYSRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHD5</accession>
    <name>G3AHD5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein YIF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_59520</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Has a role in transport between endoplasmic reticulum and Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Golgi apparatus membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the YIF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373683.1">
        <property type="nucleotide sequence ID" value="XM_007373621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHD5"/>
    <dbReference type="FunCoup" id="G3AHD5">
        <property type="interactions" value="526"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHD5"/>
    <dbReference type="GeneID" id="18872956"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59520"/>
    <dbReference type="eggNOG" id="KOG3094">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047877_2_1_1"/>
    <dbReference type="InParanoid" id="G3AHD5"/>
    <dbReference type="OMA" id="SGYKFVH"/>
    <dbReference type="OrthoDB" id="337750at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005578">
        <property type="entry name" value="Yif1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14083">
        <property type="entry name" value="YIP1 INTERACTING FACTOR HOMOLOG YIF1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14083:SF0">
        <property type="entry name" value="YIP1D-INTERACTING FACTOR 1, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03878">
        <property type="entry name" value="YIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="222"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="62"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="343" mass="39397" checksum="124AB4D294264FF9" modified="2011-11-16" version="1">MYNPYGNVAADSYQNQYQYQPHDQSQQQSPQNLQHPQPHHPTSFGHHPQQQMYTGQPPVPTAAAQQHQQYQQHQQQQQQQQQQQQQQQHAQPGFNFLNEPAAALASQFAMSGFQQSNQYIQENFGNLSISGDINYYFQVSNSYVFSKIFLILFPYRQKDWSRISTKETGENQFLPPNRDINAPDLYIPLMSFSTYILLWAAFQGLKGDFHPELFGYLSSQTLAFSILDILIFKTGLYLLGCPQSSIYDIVSFSSYKYVSIIVLLCLKHSIGDWLGLGYYVSVLVLIANLAIFLMRSLRFLILPTNNTSAATNSITSRQRKIRIQFLFVYAVIIQGLIILYMSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHD9</accession>
    <name>G3AHD9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Essential protein Yae1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_59524</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LTO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373688.1">
        <property type="nucleotide sequence ID" value="XM_007373626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHD9"/>
    <dbReference type="FunCoup" id="G3AHD9">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHD9"/>
    <dbReference type="GeneID" id="18872958"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59524"/>
    <dbReference type="eggNOG" id="KOG4595">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136375_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHD9"/>
    <dbReference type="OMA" id="VGFQRFV"/>
    <dbReference type="OrthoDB" id="20669at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019191">
        <property type="entry name" value="Essential_protein_Yae1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052436">
        <property type="entry name" value="LTO1_adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28532">
        <property type="entry name" value="GEO13458P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28532:SF1">
        <property type="entry name" value="ORAL CANCER OVEREXPRESSED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09811">
        <property type="entry name" value="Yae1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Essential protein Yae1 N-terminal" evidence="4">
        <location>
            <begin position="22"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="151" mass="17206" checksum="3A995250807E932A" modified="2011-11-16" version="1">MTDFNIDSEEILNPEEESYKSGYKEGQDQSTNEQYIEGKQYGLQTGFQRFLVVGYIQGLVDQWRKRLSQYEGNITSLESHLRQLEELIDGIPSGNSDAEVAEYETRLTKARNKVRVISTLTKDNSNINSLDDLIKSVGGQLSTSENVDEMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHE1</accession>
    <name>G3AHE1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Oligomycin resistance ATP-dependent permease YOR1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_133715</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373689.1">
        <property type="nucleotide sequence ID" value="XM_007373627.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AHE1">
        <property type="interactions" value="275"/>
    </dbReference>
    <dbReference type="GeneID" id="18869763"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133715"/>
    <dbReference type="eggNOG" id="KOG0054">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_27_1_1"/>
    <dbReference type="InParanoid" id="G3AHE1"/>
    <dbReference type="OMA" id="KTHVKEM"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18597">
        <property type="entry name" value="ABC_6TM_YOR1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18606">
        <property type="entry name" value="ABC_6TM_YOR1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001750">
        <property type="entry name" value="ATP-binding cassette transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000010">
        <property type="entry name" value="Multidrug resistance-associated ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="377"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="420"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="797"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="840"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="918"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="942"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1030"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="153"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="499"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="804"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="1119"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="482"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="482"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="497"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1381" mass="156277" checksum="BC47B097D865A374" modified="2011-11-16" version="1">MDDITSLELGNDEYRPQKRLLTFIMRKREYPIPKDEERQPHPAQKANLIYKLFFWWLFPIMKVGYARTLQPNDLWLLTDDLKVEHYTEIFYTYLEQVRIKSKKKHIEKKCKERNEAVENSSLSIEEDLKDFVLSQWDIAEVVFLTFRKRLVSGIILACLSLCLMALTPLLTKYLIRYVEARTFGLQNSPGKAIGYSLGLTFMMFITGVMINHFFYMGLLVGGATKALLTNTILNKALKLNAKGRHKFPTGKITSMITTDLARIEIALIFQPLAIGLPVAAVIAIVILIVNIGVAAVIGIVIFLFFLAFLSVGAKKLFEYRDIVSKITDKRVNLIKEILNNLKMIKFYSWEHPYHKKVVQVRNQEVNVILKIQSLRNIIYSIALTLNGISAMIAFLILYALRGKTSSPANIFSSVSSFEMLSFFVFLIPQALSTTADMLMGFKRIGELFSAQDEERYQYYLTREDESEDAVKVTDGCFSWENFEDDVNDDESDDEKDREEREKDLERSGFKSESTFKGLQDVNLTINKGEFVVITGLVGSGKSSLLNAISGFMRCDSGEIDINGSLLLCGAPWIQNNTIRENILFGSEFDKEFYDKVVYACSLHIDLDNLPGGDFTEVGEKGITLSGGQKARINLARAVYANKDIILMDDVLSAVDSRVGKHILNQCLLGLLKDKTRILATHQLALIGQADRIIFVNRDGTIDIGTMADLNASNSEFNNLMTFSKSEDDDELDDTTDVVDEKAEYIVSSDEEEEDSHLDYNLNKDAAKGKIIADEERAVNQIKNEVYLNYIKYGAGKLTIWGFLALYLFVLAISTFCSIFTNTWLSFWVSQKFEGRSNGFYIGIYVMFNILSPIFLTISFIVLITMTTLSSKNMNLKAIDKILFAPMTFLDTTPMGRILNRFTKDTDVLDNEISENMRFFFVNAAQLVGTIILFIVFIPWIAVALPVIAFVFVLIANYYQASNREVKRLEAILRSFVFNNVNEVLSGLSVIKAYKSQTRFAKISDKLVNRANEATFIVFANQRWIAIQLEIVASCLVLLVSLLCCFKVFDISPATVGLLMTYSLSIASSLSNLIRTFTEVENYMNSVERLCHYAQKLPQEAAYTIDSTEPPSTWPREGGIEFDKVNMRYRPELPLILKGLTFTIAPHEKVGICGRTGAGKSSIMTALYRLSELETGKILIDGVDISKIGLKSLRSHLSIIPQDPVLFNGTIRSNLDPFGEHSDEWLWNSLRKSGILTADEIVEAQKLDKSTRSENLPKFHLDKVVDNEGENFSLGERQLVSFARALVRESKILILDEATSSVDYETDHKIQQTIASEFSDCTILCIAHRLKTIINYDRILVMDKGQVSDFDSPWKLFNTPGSLFREMCDKSNITEEDFVNRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHI8</accession>
    <name>G3AHI8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">3-hydroxyanthranilate 3,4-dioxygenase</fullName>
            <ecNumber evidence="9">1.13.11.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">3-hydroxyanthranilate oxygenase</fullName>
            <shortName evidence="9">3-HAO</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">3-hydroxyanthranilic acid dioxygenase</fullName>
            <shortName evidence="9">HAD</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Biosynthesis of nicotinic acid protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">BNA1</name>
        <name evidence="10" type="ORF">SPAPADRAFT_135529</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>3-hydroxyanthranilate + O2 = (2Z,4Z)-2-amino-3-carboxymuconate 6-semialdehyde</text>
            <dbReference type="Rhea" id="RHEA:17953"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:36559"/>
            <dbReference type="ChEBI" id="CHEBI:77612"/>
            <dbReference type="EC" id="1.13.11.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the 3-HAO family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.6" evidence="9"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373736.1">
        <property type="nucleotide sequence ID" value="XM_007373674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHI8"/>
    <dbReference type="FunCoup" id="G3AHI8">
        <property type="interactions" value="152"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHI8"/>
    <dbReference type="GeneID" id="18869898"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135529"/>
    <dbReference type="eggNOG" id="KOG3995">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095765_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHI8"/>
    <dbReference type="OMA" id="KPPVGNQ"/>
    <dbReference type="OrthoDB" id="204928at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00330"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000334">
        <property type="term" value="F:3-hydroxyanthranilate 3,4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006569">
        <property type="term" value="P:L-tryptophan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06123">
        <property type="entry name" value="cupin_HAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000093">
        <property type="entry name" value="3-hydroxyanthranilate 3,4-dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00825">
        <property type="entry name" value="3_HAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010329">
        <property type="entry name" value="3hydroanth_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03037">
        <property type="entry name" value="anthran_nbaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15497">
        <property type="entry name" value="3-HYDROXYANTHRANILATE 3,4-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15497:SF1">
        <property type="entry name" value="3-HYDROXYANTHRANILATE 3,4-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06052">
        <property type="entry name" value="3-HAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 9" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>O2</name>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="170" mass="19485" checksum="4E620EFD9ADF7086" modified="2011-11-16" version="1">MALGQPINLKKWIEENGELLQPPVNNFCLHRGGFTIMIIGGPNERNDYHINQTPEYFYQLKGTICLKVVDDGEFKDIYIHEGDSFLLPANTPHNPCRYENTIGLVVEQDRPEGMNDGIRWYCAKCENKIFEKEFYLTDLGTQIKDAIVAFDSNLDARTCKKCGHVNISKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHL1</accession>
    <name>G3AHL1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_148682</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373759.1">
        <property type="nucleotide sequence ID" value="XM_007373697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHL1"/>
    <dbReference type="FunCoup" id="G3AHL1">
        <property type="interactions" value="529"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHL1"/>
    <dbReference type="GeneID" id="18870795"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_148682"/>
    <dbReference type="eggNOG" id="ENOG502R8BB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005934_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHL1"/>
    <dbReference type="OMA" id="HGPFTWH"/>
    <dbReference type="OrthoDB" id="2943660at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF12">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="17"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="905" mass="104768" checksum="BAE6AFA17E06C8BC" modified="2011-11-16" version="1">MTDDERPKKRRNRSTLVCNVCKQRKVKCDRKLPCTRCIIYKTQDICSYHEDLPSGQVLPPQNTVSPETLQPPNATTPDIHPNSVNNQNFLSKGPSAKVYIITSEISHILSKFTNAESVIGINPISSPEEEFSFFDYSSFSQRWDKQQLNHGPFTWHAFLDKDVALADLWTYLSLKSNELRNLSRAHSSSPGSTQPDEQQVEEDITHADLGDVLAKIPFQVLQRLNLSSFKFDAKKQKINSSAIPLGMNFTTNQEYFNDNSIDLKEKILKILPCREIIWIHINRFFKFLYPFIPLIDEATFKTEIERLLDGSGEHITKINIKDNLDYANIGMLLIILRMSFLSLICNNEEFNNMVLQSATCPKAYQSQFSPAELRNLQLLLKNCIGIEFIELARNCITKFQLFQKSNLVILQFTLLMRLYFYFSPEDAEGPDRNQFQIYNGTLIQMGYTIGLNRDPDKLGKFDKRTSNIRRKVWHFLVYLDLFQAASYGSPISTNPQYSDTKFPYYEEGNHNVSFEHFDKFLQATYENVKDLPTHLRSILMLVLDVNSKLKMGDLTDKLSALEIYVAKNLGSSLKVFADAINQVQESESDMLARVFYIPYFLQTKTFLTATYFHMFIHFEDTNLDLSYFYLKKIICVMVEDFLPQIFRLLDHQHYFFCHSTNFFINPPIECCLHNSNGFLFAIIIRIEYTIRMMKQQELNEDTKRYISSCETLIYWLTKCTKISTVGISKLGKRYLYAWRISKSHTFILKSLSSEDYFKKLNDPVECAEFAAANPKLTKFNLKLWQINELSELVEKTMMSLNKSQLINEWIELFGPISENKPKPMTPHQPADPCIPIVTDPFIGNLDNLFNQVTSRIDPGVFDTTGLNSQSNVITSTMENYLALDNPYFDIFNDMSLDQLIKSFDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHP7</accession>
    <name>G3AHP7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Flavodoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_59643</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Flavodoxin-like protein (FLP) that plays a role in cell wall integrity, oxidative stress protection and virulence. FLPs act as NAD(P)H quinone oxidoreductases. Reduces ubiquinone (coenzyme Q), enabling it to serve as an antioxidant in the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WrbA family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373795.1">
        <property type="nucleotide sequence ID" value="XM_007373733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHP7"/>
    <dbReference type="STRING" id="619300.G3AHP7"/>
    <dbReference type="GeneID" id="18873001"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59643"/>
    <dbReference type="eggNOG" id="KOG3135">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051402_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHP7"/>
    <dbReference type="OMA" id="HGMPYIP"/>
    <dbReference type="OrthoDB" id="504689at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032126">
        <property type="term" value="C:eisosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160020">
        <property type="term" value="P:positive regulation of ferroptosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000001">
        <property type="entry name" value="NAD(P)H dehydrogenase (Quinone) FQR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010089">
        <property type="entry name" value="Flavoprotein_WrbA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01755">
        <property type="entry name" value="flav_wrbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002999">
        <property type="entry name" value="PRK03767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546">
        <property type="entry name" value="FLAVODOXIN-RELATED PROTEIN WRBA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546:SF23">
        <property type="entry name" value="FLAVOPROTEIN-LIKE PROTEIN YCP4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0843">Virulence</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="6">
        <location>
            <begin position="3"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="240"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="240"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="264"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00053955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="287" mass="29885" checksum="93D8C930745AD88C" modified="2011-11-16" version="1">MKVAIIQYSTYGHITQLARSVKAGVEESKLASTVDIFQIPETLSDEILGKMHAPPKPKDIPVATLETLEEYDAFIFGIPTRFGTAPAQYFEFWGATGGLWMKGALDGKPAGIFVSTGTPGGGQETTVRLSLSHLAHHGMPYIPLGYAKTYAQVTADEVHGGSPYGAGTYANGDGSRQPSEVELEMAKIQGTDFATSAIKFVGKKAATKTAAAAGSSASATKKTVDDSVAPPAASDLANNAKGEAKAAAANTQAAASKTQAAASKRAEQSKQTVESEEKGFCSKCTIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIL9</accession>
    <name>G3AIL9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59097</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373318.1">
        <property type="nucleotide sequence ID" value="XM_007373256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIL9"/>
    <dbReference type="GeneID" id="18872771"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59097"/>
    <dbReference type="eggNOG" id="ENOG502QYJ2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2428448_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIL9"/>
    <dbReference type="OrthoDB" id="4094942at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="91" mass="10240" checksum="F4A610F3EE05164C" modified="2011-11-16" version="1">MSSESSFETPPPEPALSTPHLKAHDDFNTIDADQTLSSIAEKKQPSVFDIAQDMQEQLKKASEFLDKNDKEMNERIDGLLKTIDHIHKSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIP0</accession>
    <name>G3AIP0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_65592</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374040.1">
        <property type="nucleotide sequence ID" value="XM_007373978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIP0"/>
    <dbReference type="STRING" id="619300.G3AIP0"/>
    <dbReference type="GeneID" id="18875048"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65592"/>
    <dbReference type="eggNOG" id="KOG2809">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082196_1_0_1"/>
    <dbReference type="InParanoid" id="G3AIP0"/>
    <dbReference type="OMA" id="KTTHDHT"/>
    <dbReference type="OrthoDB" id="3366546at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050656">
        <property type="entry name" value="PINX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149:SF26">
        <property type="entry name" value="PROTEIN TMA23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="26513" checksum="9947A5D358171F0B" modified="2011-11-16" version="1">MNASVYLQSFGWKEGEALQKGGLKKPILVKHKKDTKGLGHDYNRDGDVWWERLFDGQLKNLDVNKDNNGSVSFEQNADNVIGTVKRSMSPLYRMFVKGEGLAGTVGKTDFTAKQMEVTSSQALREMEILIEKASGDKGSKEEKKEKKEKKDKKDKKVAAKKKSKDKEKSKEKKQKKDKKEKKEKKDKKEKKDKKEKEKREKKKSIETKSKKRSRDSEDNSNSIKKRKSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIT5</accession>
    <name>G3AIT5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Signal recognition particle 54 kDa protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_134434</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Signal-recognition-particle (SRP) assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum (ER) membrane. SRP is required for the cotranslational protein translocation for ER import and preferentially recognizes strongly hydrophobic signal sequences. It is involved in targeting the nascent chain-ribosome (RNC) complex to the ER and is proposed to participate in the arrest of nascent chain elongation during membrane targeting. SRP54 binds to the signal sequence of presecretory protein when they emerge from the ribosomes. SRP54 interacts with the scR1 RNA and mediates the association of the resulting SRP-RNC complex with the signal recognition particle receptor (SR) via its alpha subunit SRP101. Both, SRP54 and SRP101, are locked in their GTP bound forms in the SRP-RNC-SR complex, which dissociates upon transferring the signal sequence to the protein-conducting channel (translocon). After signal sequence transfer, SRP54 and SRP101 act as reciprocal GTPase-activating proteins (GAPs), thereby resolving their association.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Fungal signal recognition particle consists of a 7S RNA molecule (scR1) and at least six protein subunits: srp72, srp68, srp54, sec65, srp21 and srp14.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The M domain binds the 7SL RNA and the signal sequence of presecretory proteins.</text>
    </comment>
    <comment type="domain">
        <text evidence="13">The NG domain, also named G domain, is a special guanosine triphosphatase (GTPase) domain, which binds GTP and forms a guanosine 5'-triphosphate (GTP)-dependent complex with a homologous NG domain in the SRP receptor subunit srp101. The two NG domains undergo cooperative rearrangements upon their assembly, which culminate in the reciprocal activation of the GTPase activity of one another. SRP receptor compaction upon binding with cargo-loaded SRP and GTPase rearrangement drive SRP-mediated cotranslational protein translocation into the ER.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374085.1">
        <property type="nucleotide sequence ID" value="XM_007374023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIT5"/>
    <dbReference type="FunCoup" id="G3AIT5">
        <property type="interactions" value="1022"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIT5"/>
    <dbReference type="GeneID" id="18869815"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134434"/>
    <dbReference type="eggNOG" id="KOG0780">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_6_1_1"/>
    <dbReference type="InParanoid" id="G3AIT5"/>
    <dbReference type="OMA" id="MTIFVMD"/>
    <dbReference type="OrthoDB" id="10250817at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17875">
        <property type="entry name" value="SRP54_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000022">
        <property type="entry name" value="Signal recognition particle 54 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="entry name" value="Signal recognition particle, SRP54 subunit, M-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006325">
        <property type="entry name" value="SRP54_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01425">
        <property type="entry name" value="SRP54_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 54K PROTEIN SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF5">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="Signal peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10 13" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="14">
        <location>
            <begin position="286"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW34501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="559" mass="60580" checksum="A668553F6AFD72D6" modified="2011-11-16" version="1">MVLADLGARLRGALSSVESGSDDEIQQMIKDICGALLESDVNIKLVAKLRDNIRSQIASSNIAQEASPMNKRKKLQKVIYDELCGLVDSHVEPPKPKKINASTKTIKGKKVKLSKESSHVIMFVGLQGAGKTTSCTKLAVYYKKRGFTVGLVCADTFRAGAFDQLKQNAIKANIPYYGSYLEPDPVKIAFEGVSKFKQEKFDIIIVDTSGRHRQEQQLFTEMVQIGDAIQPTQTIMVMDGSIGQAAESQARAFKESSNFGSIILTKMDGHAKGGGAISAVAATKTPIVFIGTGEHIGDLEVFKPTTFISKLLGIGDIQSLIEHVQSLNLQNDEGHKQTIENIKEGKFTLKDFQTQMNNFLKMGPLTNIASMIPGLGNIMSQVGEEETSKKIKNMIYIMDSMTQKELESDGRIFIDQPSRIIRLAKGSGCSAIEVEMILQQHRMMSTMAKSAMAAQGAPGAGGAPGMNPQMARMMQQAQSNPNFMEQAMKMMGGAGAGAGGAGGPGGLAGMMNNPQMMQQAQEMMRSNPQMMQQAQQMMKNPGMMQRMMQQFGGMGGMGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIZ3</accession>
    <name>G3AIZ3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_59162</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373388.1">
        <property type="nucleotide sequence ID" value="XM_007373326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIZ3"/>
    <dbReference type="FunCoup" id="G3AIZ3">
        <property type="interactions" value="1087"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIZ3"/>
    <dbReference type="GeneID" id="18872800"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59162"/>
    <dbReference type="eggNOG" id="KOG0181">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_4_1_1"/>
    <dbReference type="InParanoid" id="G3AIZ3"/>
    <dbReference type="OMA" id="ATCIGKD"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03750">
        <property type="entry name" value="proteasome_alpha_type_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000048">
        <property type="entry name" value="Proteasome subunit alpha type-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 6 7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="8">
        <location>
            <begin position="5"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="250" mass="27684" checksum="C092BAC452BD7DA8" modified="2011-11-16" version="1">MADRYSFSLTTFSPSGKLVQIEYALNAVKQGVTTIGIKCDNGVVLATERKSNSHLIKSETSSKVEYITPSIGMTYSGMGPDFRVLVDKARKLAHTNYKRIYNEYPPVRIMVQEIAKVMQESTQSGGIRPFGVSLLVGGYDEYSEVFQLYQIDPSGSYFPWKATAIGKTSNSAKTFLEKRWNENLQLEDAIHVALLALKEAVEGELTGENLDIAIISDPQPQLLGFKGTSVEGPRFRKLSAEEINDTLDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ04</accession>
    <name>G3AJ04_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_65646</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374100.1">
        <property type="nucleotide sequence ID" value="XM_007374038.1"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ04"/>
    <dbReference type="GeneID" id="18875054"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65646"/>
    <dbReference type="eggNOG" id="KOG0108">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_247545_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ04"/>
    <dbReference type="OrthoDB" id="272703at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="26"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="146"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="555"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="654"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="921"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1017"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1075"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1127"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1442"/>
            <end position="1505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="158"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="559"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="589"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="656"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="675"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="706"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="717"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="746"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="756"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="797"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="859"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="894"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="921"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1017"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1043"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1075"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1449"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1482"/>
            <end position="1496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1525" mass="172396" checksum="C365EAB9629F3A41" modified="2011-11-16" version="1">MNGVDNSIHGGKRGLPPTGPLAAASCILWVGAVPYEWDEETIKSVVCGSGNIVDVRVLFDSPVKNKGFCFIEYETPYDAVRAKRLISKIAIKALNGTKRLRVEISKDPPKWTDPALHERPIMQLNRGKLPENVKLPWEMTTASEGISSKNGDFNTHPGMKRTWSKRRGAKRGRGRPFTNISQVFHVNNLNHQPIQSGNSRFSNKVDIEEYSYPENKQYEDLFAQTDSRGSNKTIDLFPPVNVHHKGNDENLFANRVSREQDDDLFANRIESGANDVLFPLANSSSIPLSDQAGSVVNDKSDNDPSEANPNIDVDSCSKHDELKQPLYNHNQVQTDEINQAKHTHDATQAKQTHAGHGPLKPDIIICNNNSPLGIQDQIKTAELRIMLKLKGWLSKCKLEPQEDIPQTILAKYLSESSTSWLGDICRFLFLNYNKLRIFQEQDPEYNTKSSLFQFYLLTFSQGRAKYLDNEIHEKFFHIFLSILSTYKRVYNEIQYGYNFKIPFGIDVDTFAEHILNTKEDEERLQEMRRIEAIYNSGEMTHDSHLAVTGKPSPILAQIPLPPPLTPPPPSSTPPLPHPPSRQPSRSLVLPPPPPPPPPPPLPPQEEIDSSSDEFNQSRKLEPLVSSLKETKNSEIRGVLKDTVVVGAGEVLPDYDEVSHIDESQTKRRKTTTQIESDDSFEPEAENAKIAEESVGNDPTKVISNTKESERANDHPTKSSNFPYLQNRNGSIDSTNGAQPHTSPDLNLEKSLEESFRTTLKRIQSPQDEQEVLIIQARKERQRKKNKELLKQFNKNQTEVRRHGEVRRFKESCRDNHDDTRVTRRHSDEHIGEKSPVHTRTPPVNEMSSKRALSMRRSTSHKEGKEKEMLKHRPFGNEISSRHSEKSPDAGISNEYEKEKNRNEEKSQEVIVIHDVKLTIQQNSKSPDAINESPQTVENAHDDDMDDSLIETSSEIFEQQKQKRKGIVIDLSTNNELGIVQNEETDDVNAVIAPVETGNEISDKLSNKLDDLLHETENMTQNEKIRNQATYEQSGSEENVTNEELQKKATSDKEDRSYTVELVVCQDDASVQETEQINPISGDTSEPSCGKATDGSTSLNTVRVTLSPQVHDNNEIVGDVDQNRIDEVSQHQQSSTERNEEATSSKHQIGQLEQAGQPQEGRSETVIAVEKLRDQSEKENIQALEGYGQELTDFFSFSQQGSQPLFVAPPAQQHQQAHVVDQQRKSSGIEQQLQEIREAQLQEHEKQHVDMVSEEKERLARVHMVQAQISQSQRVAAAQKQTQKEYERLQQEFSVKASEAAKDLQRQIELVNFKQKQYLEELTKRARAEIDQIKEKHQAYMKSLKNSYLSQAVTINAASRNTGNQAPLPTFNLIPQYNQFSSDNNISMCWHSGQTNLQSYSVVPPQQNALNQQTSPNNTSLVQQQSPTFGSFVLPSRDVMMQPTARSASQLPQQNLPRLPQEGAPNSQTSGKLSYLEKRYRYDSSNVGTSRLSTRTSFAPRRSPTKREVVDVYIPPEIHTLLNSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ30</accession>
    <name>G3AJ30_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">M-phase phosphoprotein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_149578</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374126.1">
        <property type="nucleotide sequence ID" value="XM_007374064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ30"/>
    <dbReference type="STRING" id="619300.G3AJ30"/>
    <dbReference type="GeneID" id="18870830"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_149578"/>
    <dbReference type="eggNOG" id="ENOG502SFE2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151464_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ30"/>
    <dbReference type="OMA" id="FSINIRC"/>
    <dbReference type="OrthoDB" id="4084022at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10175">
        <property type="entry name" value="MPP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="13682" checksum="0A8A24670D8FA292" modified="2011-11-16" version="1">MSSLSNRVMNMKFMQKADDLKISEEREQNQKKIADLSEWVLPYSKSLLKMAQSKPKIESVGYGSIMSAPTRRSWGTKVEQEATPSPEKEASKNKSEDLNELWGKRTKSNNANSSKKRRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ94</accession>
    <name>G3AJ94_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mitochondrial ribosomal protein S19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_59214</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373435.1">
        <property type="nucleotide sequence ID" value="XM_007373373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ94"/>
    <dbReference type="FunCoup" id="G3AJ94">
        <property type="interactions" value="138"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ94"/>
    <dbReference type="GeneID" id="18872822"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59214"/>
    <dbReference type="eggNOG" id="KOG0899">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144911_1_0_1"/>
    <dbReference type="InParanoid" id="G3AJ94"/>
    <dbReference type="OMA" id="EYVAFEV"/>
    <dbReference type="OrthoDB" id="2043at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="entry name" value="30s Ribosomal Protein S19, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_uS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_uS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_uS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_uS19_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="RIBOSOMAL PROTEIN S19P FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880:SF8">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="Ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW33851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="90" mass="10294" checksum="01D7C2FB155EAC2E" modified="2011-11-16" version="1">MRPTLNILTRSVWKGPNVVPLPIAHAIKTKTPVRTNARSCTILPQFVGLRFQVHNGKEYVEFEIVDDMVGSKLGEFAPTKKRWTYRGEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJF0</accession>
    <name>G3AJF0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Extracellular membrane protein CFEM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_49638</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374193.1">
        <property type="nucleotide sequence ID" value="XM_007374131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJF0"/>
    <dbReference type="GeneID" id="18871357"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49638"/>
    <dbReference type="HOGENOM" id="CLU_1185656_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJF0"/>
    <dbReference type="OrthoDB" id="10554199at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013266003" description="Extracellular membrane protein CFEM domain-containing protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="25416" checksum="7E7EC6EAEBF32841" modified="2011-11-16" version="1">MKLTSLILYATVTVASNFVPFTEETIELLKRAEDEECGKSCVKAGDLQVKQCKDTYESNNALDVNECVCRLGDEYWELLSTCVKGCDEYRGSNSAEDPTQLKKNYCQAVKVHTDFDDGIETSKDDKTSDDDKQSSKTKDKSDDKETKTTTGDKSETDKDSKTLTSDKNSKTTDKDKSETDKSGSATKTTDKDTKTTSDSSATDEPNSKSESSTGAAGTAVWGAGSLFLVAIAML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJG7</accession>
    <name>G3AJG7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Outer spore wall protein RRT8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_148043</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373453.1">
        <property type="nucleotide sequence ID" value="XM_007373391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJG7"/>
    <dbReference type="FunCoup" id="G3AJG7">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="GeneID" id="18870772"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_148043"/>
    <dbReference type="eggNOG" id="ENOG502QVX4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062645_3_0_1"/>
    <dbReference type="InParanoid" id="G3AJG7"/>
    <dbReference type="OMA" id="IFFMFTN"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005619">
        <property type="term" value="C:ascospore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059112">
        <property type="entry name" value="CysZ/EI24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052786">
        <property type="entry name" value="Spore_wall_assembly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292:SF2">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07264">
        <property type="entry name" value="EI24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW33869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="25473" checksum="1885F821CA9DFA13" modified="2011-11-16" version="1">MIPQLGLTLVIYSCSFIVLFPIEAPIYTLLMGPFGIVAAWYSILSQAGALSLLIVNLFLMPHIQRVAYDAVLSREFADDVVLMAKIRRAGNVPLYIRVVRYVLMLPDLSSLPNTIAKAVAIFILNFIPVIGPVFTIVIQSGSRGLQGHQRYFQLKGYTRRQKDDIFRAHRAEYMGFGIAALILELIPVINLFLVFTNTIGAALWAVDIERKTKARETSAEECCIDPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJH8</accession>
    <name>G3AJH8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bms1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_135341</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class translation factor GTPase superfamily. Bms1-like GTPase family. TSR1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373465.1">
        <property type="nucleotide sequence ID" value="XM_007373403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJH8"/>
    <dbReference type="FunCoup" id="G3AJH8">
        <property type="interactions" value="1051"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJH8"/>
    <dbReference type="GeneID" id="18869885"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135341"/>
    <dbReference type="eggNOG" id="KOG1980">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009858_1_0_1"/>
    <dbReference type="InParanoid" id="G3AJH8"/>
    <dbReference type="OMA" id="MNLPRFK"/>
    <dbReference type="OrthoDB" id="119302at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000461">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012948">
        <property type="entry name" value="AARP2CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007034">
        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030387">
        <property type="entry name" value="G_Bms1/Tsr1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF1">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN TSR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08142">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22298">
        <property type="entry name" value="Tsr1_G-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00785">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01362">
        <property type="entry name" value="DUF663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51714">
        <property type="entry name" value="G_BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Bms1-type G" evidence="6">
        <location>
            <begin position="80"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="763" mass="86982" checksum="34C28F95AFD51A91" modified="2011-11-16" version="1">MAGHRNTLKHDHKPFKSKHASKGKLKNQYKGKVEKTSGAKSNSTKPVSKIERKNIAKQLKEQKILESKLTRKLFEGNQGAEKIITVVSLTSDIPPVDIANRLFNQDGANIQFAYPSVNSMHVSRFKSNLKVIIPNQDNFLHILDAAKVSDFVIFGISANQEVEKEYGEQILRSLIAQGIASVIGVVPNIVQAYPKRNLQLDVKQSLQSYFNHFFSANEEKLFSIENESDNANCLRTICQKFPKGVTWRDSRGWLVADSVSASDEHLVVEGTVRGVGFNANRLVHIPGYGDFQIDRLEKIRGRKRATMDVDDVVDSYHPDENQETLDEIYPEEVDMEADDYDDFDWEGENNNLGVRTEGRIYFDDDGVKRPMEKRRLPKGTSEYQSRWLVDDVLENASDLEDEDEIQQDVLEIDEEPMEATATEYATTEVGDATDMHVDLSPEEETRQLEEFRALEKDDLDFPDELELHPNESAKERLAGYRGVKSLGNCNWDVDENDIEAPSIWNRLLRISNYKATKNRISKEYIKQTEVTGGNKVRIYVRAPVQLILERVNPAVQPFTIYELLEHEHKLAVCNFTFESWEDYEKPIPSRESIIVQYGPRRQIIEPTFNQASNNPNNVHKSENFAHLGATVICTAITPVLFNQAPVIYFKPSGNNIEFIGQGTFLNCDHTRIIAQRAVLTGHPVKIHKRVVTVRYMFFNPEDINYFKAISLFTKSGRTGFIKESLGTHGYFKANFDGKLTSQDVVAMSLYKRLWPTISSAWTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJJ7</accession>
    <name>G3AJJ7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59276</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373484.1">
        <property type="nucleotide sequence ID" value="XM_007373422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJJ7"/>
    <dbReference type="STRING" id="619300.G3AJJ7"/>
    <dbReference type="GeneID" id="18872847"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59276"/>
    <dbReference type="eggNOG" id="KOG0431">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1409607_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJJ7"/>
    <dbReference type="OrthoDB" id="1717591at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072318">
        <property type="term" value="P:clathrin coat disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23172">
        <property type="entry name" value="AUXILIN/CYCLIN G-ASSOCIATED KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23172:SF19">
        <property type="entry name" value="J DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="22146" checksum="783234CCD97E2376" modified="2011-11-16" version="1">MKLISTKAEVLELLERYESSLQLYNLLIQRLGTKDKKIMDGRRRVDKIVNPENHIKKPQPQRPKTGSSVPTPSATPKPVQQEEEVDTLVKDKIDTKIQAWATSKQNNLRAMLTNLDEIIPGKINMKPALRKLSLNDLMLSKQVKIQYMKVISSIHPDKLASQCKGDKETELICNGVFITLNKRWEAFRKEENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK18</accession>
    <name>G3AK18_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59496</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373653.1">
        <property type="nucleotide sequence ID" value="XM_007373591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK18"/>
    <dbReference type="GeneID" id="18872942"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59496"/>
    <dbReference type="HOGENOM" id="CLU_1897516_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK18"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442376" evidence="1">
        <location>
            <begin position="22"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="134" mass="13234" checksum="D79B52C35E7617EE" modified="2011-11-16" version="1">MNKVLIAYLFAFALAAPQLVGKNELVVRDVTTVLTVQVEQTITATVPDSTVDTAAAVVTETDTAAGTETGAATETTTVATQAGTETSPAGAETTVTETPVTTVPPITTVATGGAAIPVGSVAALALVALGMVGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK38</accession>
    <name>G3AK38_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60191</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374364.1">
        <property type="nucleotide sequence ID" value="XM_007374302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK38"/>
    <dbReference type="GeneID" id="18873266"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60191"/>
    <dbReference type="HOGENOM" id="CLU_1918395_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK38"/>
    <dbReference type="OrthoDB" id="10585119at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442380" evidence="1">
        <location>
            <begin position="18"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="132" mass="14384" checksum="A7068C39EE613AC9" modified="2011-11-16" version="1">MRYTYLLALGLITGVFSAPVPKNDKPICFKDSSGVVRCLGDLDSATPACFKDTEGNERCLGALDPPNPLCFKGDDGHLYCLGDLAKRENKEICFKDTEGNERCLGVLDQGANDKDEDDKICFPSHLGPTLCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKA4</accession>
    <name>G3AKA4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_60901</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375077.1">
        <property type="nucleotide sequence ID" value="XM_007375015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKA4"/>
    <dbReference type="GeneID" id="18873626"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60901"/>
    <dbReference type="eggNOG" id="ENOG502QSPS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011934_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKA4"/>
    <dbReference type="OMA" id="LTHEYND"/>
    <dbReference type="OrthoDB" id="4158994at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="22"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="394"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="541" mass="62425" checksum="99F740901327ACD7" modified="2011-11-16" version="1">MLVIDCVIYVGLTKYSDSQRMYFTYLTTLAHSIFASELIGVNSINYINTITIKKITTKIQYDNNWFINQRFINSLICFVIINYITYVLNWLNFTLRLNLTPPISQADNLYLTISIHIILQTILFKNSSIIQNSIPNLTDEVSVNVDDNKLLDIDITQLKQINPSNQSIAVKNFENFIISPINSKLSVLSKSDKVVTTNSKSNNVTTINNKIVIQPFWNIIAAFKSILKNKDLFNHAVTDDEEIADSVKVSVVMCNSNKVVLQLFDPVERLLVKLNQINWMSFKTIDNYLIIYDLASAFKYEIVLYQDDTPVNKTVVVTTGSVSFACGSGSIGTIQTSLSTTMSKLTQLRQKFKKYKRDENKRITELRNSIDVIKTRLTKQNTPKHNDIRLVGKIKGLKQTVIQLEHDIDQLSAEIKQLTIQENEISQQFEIKDEEYASEVQLLEQEYEDYLSRLNKLRAELKNIKGECQTILAKNHKLQVKQLNKQDEIKTLTNELNDIKSREILDKFKTRTRKTNDKFDYILPKLIHETEVLTKMFSDRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKG4</accession>
    <name>G3AKG4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Ribosomal RNA-processing protein 8</fullName>
            <ecNumber evidence="9">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_60265</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(1) position of adenine in helix 25.1 in 25S rRNA. Required both for ribosomal 40S and 60S subunits biogenesis. Required for efficient pre-rRNA cleavage at site A2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the methyltransferase superfamily. RRP8 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="9"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374436.1">
        <property type="nucleotide sequence ID" value="XM_007374374.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKG4"/>
    <dbReference type="FunCoup" id="G3AKG4">
        <property type="interactions" value="305"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKG4"/>
    <dbReference type="GeneID" id="18873304"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60265"/>
    <dbReference type="eggNOG" id="KOG3045">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027694_1_0_1"/>
    <dbReference type="InParanoid" id="G3AKG4"/>
    <dbReference type="OMA" id="FTNTEHI"/>
    <dbReference type="OrthoDB" id="10258825at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106142">
        <property type="term" value="F:rRNA (adenine-N1-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2150:FF:000001">
        <property type="entry name" value="Ribosomal RNA-processing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2150">
        <property type="entry name" value="Ribosomal RNA-processing protein 8, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007823">
        <property type="entry name" value="RRP8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042036">
        <property type="entry name" value="RRP8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787:SF0">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05148">
        <property type="entry name" value="Methyltransf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="22"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="323" mass="36028" checksum="951364A9E639C6C9" modified="2011-11-16" version="1" fragment="single">MTLFEVKGWELKQEKIAVGGVVSKKKAKKEKKEKKRQAEAEANAAVQNAETAAITEVVANDQAEEKPQESKKRKADSEHTQTKKSKTKQVEAPAPIVSTTTKLTPLQQKMMAKLSGSRFRWINEQLYTISSESALELIKSQPSLFDEYHAGFRSQVASWPENPVDVFVKQIKTRATTRNVNAPGGLPGLANRKVVVADMGCGEAQLSLDVSKFLAQHNKKNKRKLDIQVHSFDLKKHNERITVADIKNVPLPDESCTIVIFCLAMMGTNFLDFVNEAYRILAPNGELWIAEIKSRFTESGNNANPEDVGQEFVDALKLCGFFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKI2</accession>
    <name>G3AKI2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Squalene synthase</fullName>
            <shortName evidence="20">SQS</shortName>
            <shortName evidence="20">SS</shortName>
            <ecNumber evidence="20">2.5.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">SPAPADRAFT_60279</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>2 (2E,6E)-farnesyl diphosphate + NADH + H(+) = squalene + 2 diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:32299"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15440"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:32300"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>2 (2E,6E)-farnesyl diphosphate + NADPH + H(+) = squalene + 2 diphosphate + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:32295"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15440"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:32296"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 20">Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 20">Belongs to the phytoene/squalene synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.21" evidence="20"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374454.1">
        <property type="nucleotide sequence ID" value="XM_007374392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKI2"/>
    <dbReference type="FunCoup" id="G3AKI2">
        <property type="interactions" value="322"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKI2"/>
    <dbReference type="GeneID" id="18873314"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60279"/>
    <dbReference type="eggNOG" id="KOG1459">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031981_2_1_1"/>
    <dbReference type="InParanoid" id="G3AKI2"/>
    <dbReference type="OMA" id="GEACQLM"/>
    <dbReference type="OrthoDB" id="431150at2759"/>
    <dbReference type="UniPathway" id="UPA00767">
        <property type="reaction ID" value="UER00751"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051996">
        <property type="term" value="F:squalene synthase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045338">
        <property type="term" value="P:farnesyl diphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902767">
        <property type="term" value="P:isoprenoid biosynthetic process via mevalonate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00683">
        <property type="entry name" value="Trans_IPPS_HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000003">
        <property type="entry name" value="Farnesyl-diphosphate farnesyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006449">
        <property type="entry name" value="Squal_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019845">
        <property type="entry name" value="Squalene/phytoene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044844">
        <property type="entry name" value="Trans_IPPS_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033904">
        <property type="entry name" value="Trans_IPPS_HH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01559">
        <property type="entry name" value="squal_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11626">
        <property type="entry name" value="FARNESYL-DIPHOSPHATE FARNESYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11626:SF2">
        <property type="entry name" value="SQUALENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01018">
        <property type="entry name" value="Squalene/Phytoene_Synthase_Lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01044">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01045">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0492">Microsome</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="17" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="13" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="16" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="7 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="422"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU368088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EGW32939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="444" mass="50550" checksum="4B06805E956E4920" modified="2011-11-16" version="1">MGKLLEFLLHPDELKAVIQLLKFRQPLHPHDLTKESPELQRCYYLLTKTSRSFAAVIEELHPELRDAIMIFYLVLRALDTIEDDMSIKSEIKVPLLRTFDEKLNTKDWTFDGNGPNEKDRLVLVEFDKILSIYHGLKPQYQDVVKDITYKMGNGMADYILDENFNLNGVETVKDYDLYCHYVAGLVGEGLTKLTVMAGFSDDSLAQDNFEKSNSMGLFLQKTNIIRDYHEDLLDGRSFWPKEIWSKYTKELPLFHKDASFEQAGLSCINELVLNALSHAKDCLSYLSMVKDPSTFSFCAIPQVMAIATLAEVYNNRKVLHGVVKIRKGTTCKLILESRTFPGVVAIFRRYIQVINHKSSVSDPNYLKIGIKCGEIEQFCEVMYPSVEPPKGSKIAANPIHDVLKSRQAIDDDMQMRIIKETILTNSVLVVIAVLAISSVAYLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKJ5</accession>
    <name>G3AKJ5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_60936</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375115.1">
        <property type="nucleotide sequence ID" value="XM_007375053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKJ5"/>
    <dbReference type="GeneID" id="18873643"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60936"/>
    <dbReference type="HOGENOM" id="CLU_1598537_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKJ5"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="167" mass="18174" checksum="D6A9FB56CE26AC71" modified="2011-11-16" version="1" fragment="single">LQNIHPYSTTNSNELIMSAPKSIELGDVTYDDSSDNIGVLYQFERINVNVDGNIMYELRKFGYDENVKTHGFLHDQNDNLVALVYERVYPGSNEAIVATGVAGASGGAGTVGTVGAVGTVGTVSGGRKHDRIWKELWGVFKPWIEISIISTIVVCEIIVSIDKIRGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKU7</accession>
    <name>G3AKU7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_152051</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375162.1">
        <property type="nucleotide sequence ID" value="XM_007375100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKU7"/>
    <dbReference type="GeneID" id="18870915"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_152051"/>
    <dbReference type="HOGENOM" id="CLU_1907997_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKU7"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442208" evidence="2">
        <location>
            <begin position="19"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="133" mass="15538" checksum="751A3C82115016FB" modified="2011-11-16" version="1">MKITLLILYLFLISMVTADYPRIHSHFGDGYGTDSSSEEEYKSPKYFSFKKLFLRLLFQDMFSNDYDSGSDDDSASEPDWKFSDPYWESSSESSDDEQHFRHGIDKRSDFSRIKMDGSILLNRSLNYPINILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKW4</accession>
    <name>G3AKW4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Extracellular membrane protein CFEM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_66021</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374522.1">
        <property type="nucleotide sequence ID" value="XM_007374460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKW4"/>
    <dbReference type="GeneID" id="18875075"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66021"/>
    <dbReference type="HOGENOM" id="CLU_987540_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKW4"/>
    <dbReference type="OMA" id="QQFITGA"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442428" description="Extracellular membrane protein CFEM domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="249"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="282" mass="29426" checksum="AB392FF62C7F9812" modified="2011-11-16" version="1">MKYIYILSFAILSSGLVIQKRADCMISCSAQAEADTPVCGELTGEEYDACICAQDHQYWENIWTCVVGLCPEYSADLDLNGAEAKAKVCAAPPEPQPETTATEAETTAPEVEPPATTNAETESSEEPKPEDPTDVPTETGTEQPAETGTEPPAETGTEQGTEQPTESATETGAATETGAATNTDAPETTTVTEECSSVECAEETSVSESKSVVTITESCTESQVCTKTVTICEGKPCPTTKPEEPTKPTPTTKPTVAPTEVPNGAAIAGVGSIMYLLVAAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL16</accession>
    <name>G3AL16_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RanBD1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_60376</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374574.1">
        <property type="nucleotide sequence ID" value="XM_007374512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL16"/>
    <dbReference type="STRING" id="619300.G3AL16"/>
    <dbReference type="GeneID" id="18873375"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60376"/>
    <dbReference type="eggNOG" id="KOG0864">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058492_0_0_1"/>
    <dbReference type="InParanoid" id="G3AL16"/>
    <dbReference type="OMA" id="NTETHKG"/>
    <dbReference type="OrthoDB" id="411251at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF142">
        <property type="entry name" value="RAN-BINDING PROTEIN 3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RanBD1" evidence="4">
        <location>
            <begin position="224"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="154"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="115"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="217"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW33059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="373" mass="41449" checksum="A6C7D9D78F725D21" modified="2011-11-16" version="1">MSEESTKRKIESEGSSLDAKRTKSTNDDLRQSIIQEVTKKLESRIDKLESRIDKLEREFASDDEDDDSYKEGQTLTDDDNDNDVATKTTPTDPPQLPKREGSVPLPAGETKKATFGTSSFKFTPSKLGSETPVSPPKTNKPVFGATTMFGKSIADDLKNRPSVCDTLPSKLEASKLDSSSSQDDKTSSPPPKVPKFGSHSKFGNAFQESLHKKSFLDSNDTEKDDKSATPPPQQQFKQVDLAPVHLATGEEDEISHFNSVAKIFELDLTAVNEGWKERGVGPLHLNQSRKDQNQARLVMRSQGLLRVVLNYKITKDTTLLKGLEASLSPGKYLRLNSVKDGKPVQYMLKFSNESLRDELIEKVESLKSQIKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL40</accession>
    <name>G3AL40_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphoserine aminotransferase</fullName>
            <ecNumber evidence="12">2.6.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_60393</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>4-(phosphooxy)-L-threonine + 2-oxoglutarate = (R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:16573"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58452"/>
            <dbReference type="ChEBI" id="CHEBI:58538"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>O-phospho-L-serine + 2-oxoglutarate = 3-phosphooxypyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:14329"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57524"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.52" evidence="12"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374598.1">
        <property type="nucleotide sequence ID" value="XM_007374536.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL40"/>
    <dbReference type="FunCoup" id="G3AL40">
        <property type="interactions" value="615"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL40"/>
    <dbReference type="GeneID" id="18873384"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60393"/>
    <dbReference type="eggNOG" id="KOG2790">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034866_0_2_1"/>
    <dbReference type="InParanoid" id="G3AL40"/>
    <dbReference type="OMA" id="AFVYFCD"/>
    <dbReference type="OrthoDB" id="1703350at2759"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00197"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004648">
        <property type="term" value="F:O-phospho-L-serine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000082">
        <property type="entry name" value="Phosphoserine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000006">
        <property type="entry name" value="Phosphoserine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00160">
        <property type="entry name" value="SerC_aminotrans_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022278">
        <property type="entry name" value="Pser_aminoTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003764">
        <property type="entry name" value="PRK05355.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01364">
        <property type="entry name" value="serC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43247">
        <property type="entry name" value="PHOSPHOSERINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43247:SF1">
        <property type="entry name" value="PHOSPHOSERINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000525">
        <property type="entry name" value="SerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 12" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0718">Serine biosynthesis</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="13">
        <location>
            <begin position="13"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW33083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="391" mass="43010" checksum="F061E829B2F2B950" modified="2011-11-16" version="1">MSRTLEREEPNYFGAGPALLPTSVLQQAAYDLITYEGDNIGIGEISHRSKPAIKVIDDTKANLTKLYNIPDTHEVFFMQGGGTTGFSSIAYNLFANYAHKTGKKGKAAYAVTGSWSKKSAEEAERLGFDVDIVVNTKSEKYGTIPPYSEWKPIDKDTAYLYVCDNETVHGVEFKDIPGPDYLSEGVELVADMSSNILSKQIDVGKYGLIMAGAQKNIGLAGLTIYIIKKSLLEQASDETLRQLNIPIVPIAFHYPTVVKNNSAYNTIPIFTCHILKLVTDKLIEQGGVPAIEKINQEKAKILYEALQAYPDLYSLPVTNPKVRSNMNVVFRLPNEELEAKFIKQAGEKKLAGLKGHRSVGGMRASIYNAVTLDSVKLLVEFVNDFAKANSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL98</accession>
    <name>G3AL98_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inorganic phosphate transporter PHO86</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_55072</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374656.1">
        <property type="nucleotide sequence ID" value="XM_007374594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL98"/>
    <dbReference type="FunCoup" id="G3AL98">
        <property type="interactions" value="205"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL98"/>
    <dbReference type="GeneID" id="18871907"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55072"/>
    <dbReference type="eggNOG" id="ENOG502QSU5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076919_0_0_1"/>
    <dbReference type="InParanoid" id="G3AL98"/>
    <dbReference type="OMA" id="ELWGVQF"/>
    <dbReference type="OrthoDB" id="4082764at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024297">
        <property type="entry name" value="Pho86"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11124">
        <property type="entry name" value="Pho86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="40006" checksum="14347B208A08DEA7" modified="2011-11-16" version="1">MAIQKEVDLNEPLDAHEAPTLSKTPLKPEFAKAALILHGDTYKQLQAKLNRFIFWHPIAIIAYFLLIPAALLYNLWDFVEVSDSAWEFFLLGKKNPKDFIYGIIAAFPMVAGIFATFGLITYIMGDDIGNITSAFVAQKYCDKVFGFDIKKFAQLTGHETDVDSIKLLKKGLNTQIIMYRESPIAICTVVVDEEQSTPESFIVEITGLHVRKVFENVDFHELLIEWAVLRTRELYEEYAKQKKVKTQEGTILITTDAYSFDKRLQTCLYNTNFGVMSKDVELNRFSDTLNKPKEILHKLLGVSRDTFGLALSTAKEDEQIVHNLEGKFLSTEDEVVDKPQPVPANIRRRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALC7</accession>
    <name>G3ALC7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60477</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374685.1">
        <property type="nucleotide sequence ID" value="XM_007374623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALC7"/>
    <dbReference type="GeneID" id="18873434"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60477"/>
    <dbReference type="HOGENOM" id="CLU_1759933_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALC7"/>
    <dbReference type="OrthoDB" id="10566668at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="148" mass="16761" checksum="81547BDA9FD7675B" modified="2011-11-16" version="1">MSSLGSPIPDDIPLRSPLRLKNRCYGGQWGMNMLNARIQNKHRQQESITSISTFDSMNSDSSFEMPSGSSSIFSVDRSSHKINDLDNNDNDKEQEEEEEEEDTEFLDAIADAEIYSIDGIETIVGVYCLSATEFEHNRNITRLSYVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALG8</accession>
    <name>G3ALG8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_55124</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374726.1">
        <property type="nucleotide sequence ID" value="XM_007374664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALG8"/>
    <dbReference type="FunCoup" id="G3ALG8">
        <property type="interactions" value="232"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALG8"/>
    <dbReference type="GeneID" id="18871911"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55124"/>
    <dbReference type="eggNOG" id="KOG0032">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010370_1_0_1"/>
    <dbReference type="InParanoid" id="G3ALG8"/>
    <dbReference type="OMA" id="YAMYCLT"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF572">
        <property type="entry name" value="FATTY ACYL-COA SYNTHETASE AND RNA PROCESSING-ASSOCIATED KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="432"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="206"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="460"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW33211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="894" mass="100872" checksum="B4D0B91F6FAB87AF" modified="2011-11-16" version="1">MEIPLKSQPKVSTVNLLDDSKFQITPSVSPTDEDVHTQLPSNLNYKVGHSAKPTINYNLDHDSHPLPSQFRLTRRFSETEKNNFDSETGRRIFEDGKIRPHVIKHDFLERQKTFTHLYGSRADPASMTADPSTTTTNLVTLTNNENAYPFEALPMKIYSRSHDSNIDINENYRIGLEDEYIPGLDFSDMVYQWNKSTSDFEKGKQSDSNSNSAYTSHSNTPHSREESYLDLNKLHAQVAPQPIRTANPAKNFSFSKLHEYMKTRLPEGEIAESSTAKSIDTLTSTSSEKRGGVPCDDIVELPIRAKKQRPSDSVIDPVTGEINYELIVNSLPPNFNDLPYSQRKKIVKSFSDSIDYSQFSVYAKNFLGSSLGSGKTPRSGGNSANNSFVKRPRVGSVASRLLAHASTNDLKKYHEPKQKYNIDEKGAIVMGHELGKIIGFGAWGTIRECTDNAGNIRAIKIVKSIRDCDTPSSRPSSRMDFSDNRQRHNPKVLEVFKKEISIWKQLHHENILPLIEHLETENAIFCIMERIYGGTLFEMVTAMGQFDCGLHTTTGPLGFSIESQRERLLRVIDCTRQIVNALLYMHEEKGIVHGDLKLENVLLERKDDDLYQIKLCDFGMSRVYSARVSRQNSAKHLASPIASLAVDENALMMRSRSSNAGMRKPLIGGDTPNSRKLELSIHDDSRVGISNFFKTHGPSMQSIHLTPNTYPEIYDFTAGKASPSINEGVDSGLPHSHIGSLPYAAPELLLPSPPPLGPSADVWALGVLMYAMCVGKLPFQHQYEPRLRAIIAAGKFNKADLRKACLLEWVLKKEGCDEEEEAQNAFAASSMVDLRRQEEWEHLRCSWKKIEHKREFKWLYDIICGCLESNITKRWDTPKIYEHLNRALESNDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALQ1</accession>
    <name>G3ALQ1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_137141</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374809.1">
        <property type="nucleotide sequence ID" value="XM_007374747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALQ1"/>
    <dbReference type="STRING" id="619300.G3ALQ1"/>
    <dbReference type="GeneID" id="18870019"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137141"/>
    <dbReference type="eggNOG" id="KOG1502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_9_2_1"/>
    <dbReference type="InParanoid" id="G3ALQ1"/>
    <dbReference type="OMA" id="HRAQFIA"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="335" mass="37104" checksum="9A31F4AA09E9F30A" modified="2011-11-16" version="1">MTSSTVFVSGANGYIAQHIVKQLLAKNYKVIGSVRSQSKGEDLASLVNNSNFSYVVIPSISDKDAFYEVLQKHPEITVFLHTASPVTFEVDDVERDLLRPAIDGTVNALAAIKKYGPQIERVVITSSGGAVLGFDNYFESDKIYDEESWNPITYEESKSSGPVNGYFGSKKFAELAAIEFVETEKPNFDVTFVTPVYNFGPQAYEVKDKSKLNVSAEVVNTVLKFKKGDAIPEFGNIFADVRDVARAHIVAFESENSKGKRLLVGTDNFTYDTIAYIINKHFPEVEIPQGDLSKNEAIYKSMVQKYDNSKTNAILGFDLIGLEQSIVDTVKQLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALZ9</accession>
    <name>G3ALZ9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_71207</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374867.1">
        <property type="nucleotide sequence ID" value="XM_007374805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALZ9"/>
    <dbReference type="STRING" id="619300.G3ALZ9"/>
    <dbReference type="GeneID" id="18875364"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_71207"/>
    <dbReference type="eggNOG" id="KOG0048">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_426985_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALZ9"/>
    <dbReference type="OMA" id="ASNWVKI"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF25">
        <property type="entry name" value="MYB PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="76"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="80"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="128"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="132"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="429"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="231"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="458"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="504"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="545" mass="59427" checksum="55698C707E8F0BE6" modified="2011-11-16" version="1">MFKKPEQYHYPGGPEVHQQPQPQGAPPISVTGPPQFQQQQPDYQQQQQQPHPHPHHAQVQLHMNGIVSSNGNVSGTVPSRRGPWSPMEDKKLLELITIFGPTNWVRISNNLLTRTPKQCRERYHQNLKPSLNRSPITVEEGELIESLVAKYGKKWAEISRHLNGRSDNAIKNWWNGGANRRRRASLATVSSNDNVSNDPSAKRNNSISAAPNGYSEVKPGAAMLPPPPTAPVTSSTTSTSTANSTAPTSAGSTTGVSPSSSEATLSAVTAATTITTTTTNTSGSTSADSNRHTQLPQISFNTSMFGKPSLDKNGSRTSPPPHIQGPGLRSASFDMNSTTLPPINQSNKRRLIEDQFNRRHSLANPLLPLPTPSGTVYPNSSHPNLLSSYTTNPNGNVSPSYYGSPLLLSNNASRNNSISHFELLNNSTCSSSRRSSSIAPDFFPNPLNKDSTNHKRNLSQNSLFNSPSMAPLTRSSISSNVRSEDRSNSNTSINETVKEDEEEMLPRDEIKQQDSTDKDTQMDQDEETLSAPRAKIKMSVSSLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM55</accession>
    <name>G3AM55_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">calcium/calmodulin-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_54782</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.17" evidence="2"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374275.1">
        <property type="nucleotide sequence ID" value="XM_007374213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM55"/>
    <dbReference type="FunCoup" id="G3AM55">
        <property type="interactions" value="643"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM55"/>
    <dbReference type="GeneID" id="18871886"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54782"/>
    <dbReference type="eggNOG" id="KOG0032">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="InParanoid" id="G3AM55"/>
    <dbReference type="OMA" id="HDWFESR"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000449">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000278">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="45"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW32760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="458" mass="51674" checksum="FBB3187A454D48BD" modified="2011-11-16" version="1">MQFKAQTPPLAATEKPQSHSDGVQIKKLFNKLSGQPESYSRKSNYTFGKTLGAGSFGIVRYARDNGTNEEVAVKIILKKALKGNETMIIDELHLLQQLNNPHIVGFRDWFESKDKFYIVTQLATGGELFDRIVKKGRFTEHDASLVVVQMLEALAYLHSKDIVHRDIKPENVLYLTPEENSEIVLADFGIAKQLQSPNEKLTSSAGSFGYAAPEVILGTGHGKPCDIWSLGVVTYTLLCGYSPFRSENVQDFINEVKHNNAVIFHADYWKDVSKDARRFIIKALQYSPDKRPTAAELLDDPWIVTIAEKHKECDLLPHLKQGFDAKAKFRQVVEVVKLNNRIKRLKQIQSEEDDDPNEINLFNDAGSVMSTASRVPSGVEQSPLLTWKKFSNVLNNLDQDRSKASLTPLPAYAEDAKQTKKKETTADAFVQLVHAATENKERVENFDETKHEEEQQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMF9</accession>
    <name>G3AMF9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_54821</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NBP35-CFD1 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP2/CFD1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374326.1">
        <property type="nucleotide sequence ID" value="XM_007374264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMF9"/>
    <dbReference type="FunCoup" id="G3AMF9">
        <property type="interactions" value="156"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMF9"/>
    <dbReference type="GeneID" id="18871892"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54821"/>
    <dbReference type="eggNOG" id="KOG3022">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024839_0_1_1"/>
    <dbReference type="InParanoid" id="G3AMF9"/>
    <dbReference type="OMA" id="WIPVFAD"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904564">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly scaffold complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001119">
        <property type="entry name" value="Iron-sulfur cluster carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03039">
        <property type="entry name" value="NUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028600">
        <property type="entry name" value="NUBP2/Cfd1_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264:SF19">
        <property type="entry name" value="CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="20"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="277" mass="30380" checksum="3EEBDFC621D6FD83" modified="2011-11-16" version="1">MEAPPKSLNHVKHIVLILSGKGGVGKSSVTTQTALTLHAQGYSVGVLDIDLTGPSLPRMFGVEERQVHQSTNGWIPVQVCPKLYLMSLGFLISNRNNSVVWRGPKKTAMIKQFLQDVCWGSESEPLDYLLIDTPPGTSDEHIAIAEELRWANPDGAIIVTTPQQVSTADVRKEINFCNKVNFEVLGIVENMSGFICPYCAECTNIFSSGGGKQLSESLALPYLGNIPIDPSFVELIELQDNNTKGKSLVELYDKCSLKPIMNEIIGKILQKNLPSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMP9</accession>
    <name>G3AMP9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_50369</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375008.1">
        <property type="nucleotide sequence ID" value="XM_007374946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMP9"/>
    <dbReference type="GeneID" id="18871434"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50369"/>
    <dbReference type="eggNOG" id="ENOG502RQF1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1476013_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMP9"/>
    <dbReference type="OrthoDB" id="4026700at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="183" mass="20870" checksum="16DBB97C31B069B2" modified="2011-11-16" version="1">MDQFLPVQSYNAFFTSMQSCSPDILQVEDPQPLHKPCNQILFPVESKSPQNTPEFQSLFLDQPFHESNYYGEMLERSCPEQLLEKSDQQGLFPNASTNYDALSFNHEPQHMQEQIKNDEPVQIQWSSSDSSPSLSQQTTIDSDDYSDSENPVVPGLFPTMNIDDDSQFMKTDSLPLTPSYICQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN64</accession>
    <name>G3AN64_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anaphase-promoting complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_61013</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375183.1">
        <property type="nucleotide sequence ID" value="XM_007375121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN64"/>
    <dbReference type="STRING" id="619300.G3AN64"/>
    <dbReference type="GeneID" id="18873680"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61013"/>
    <dbReference type="eggNOG" id="KOG4640">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_473412_0_0_1"/>
    <dbReference type="InParanoid" id="G3AN64"/>
    <dbReference type="OMA" id="KTSIWCY"/>
    <dbReference type="OrthoDB" id="2110451at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024789">
        <property type="entry name" value="APC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024790">
        <property type="entry name" value="APC4_long_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13260">
        <property type="entry name" value="ANAPHASE PROMOTING COMPLEX SUBUNIT 4 APC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13260:SF0">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12896">
        <property type="entry name" value="ANAPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="6">
        <location>
            <begin position="22"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 4 long" evidence="7">
        <location>
            <begin position="200"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="576" mass="66739" checksum="AC8489488CF5A941" modified="2011-11-16" version="1">MTQSLSVIHSGKFVSLSDGNILTFCPSLNLLFISMNKTSLWIYRTNGERIYSINNKTPIQSICFNQDWFVCVGLDSSIKIYDCDDGKLLQTHTSENVKLIQWTSLQQLSEQLYNVDVLGQMPKLPLVENSSLDYMIVGDDHSVNITFNNLTNETQLNTSISLPDGWIPVLPPQGELFKELMFLIRRDNSHRLLCLSYNLENKTLFQTVLVKLSHSINLLHFIQKNLQDCHKHIESSFQILDRLFDNLQTEIEEPLTVHLINIALTNLIDESTKDYWLNSMGERNYKRLSKAMNHMYDEVRQVMFKFVITSFERLIILLSDLKGITIWLNDTEEQDFGLNLNIIEELISMSTSWIKTSYRLIFDIQKEQKLFNEFMAWVKGSIIDKISEEESTDQDFSICDIIKFINNLLLTSSLGKYFELCDYSILKFKSDINLLHDIKQISGKFKLLTSSVNNFHRSVIHFEETIQVELPPSHYNLTLQQAQGEWLLCYMESSSLGIKNLTHNKTHMLTNIKSYQYNNNCIFALSDELIVLDIDFNQTSIEIPPLTFVPDKLIVTNDYCLFADSDRNYSICLNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANK3</accession>
    <name>G3ANK3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein uS9m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">37S ribosomal protein S9, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_61594</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375807.1">
        <property type="nucleotide sequence ID" value="XM_007375745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANK3"/>
    <dbReference type="FunCoup" id="G3ANK3">
        <property type="interactions" value="203"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANK3"/>
    <dbReference type="GeneID" id="18873968"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61594"/>
    <dbReference type="eggNOG" id="KOG1697">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036531_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANK3"/>
    <dbReference type="OMA" id="RESAMWA"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000001">
        <property type="entry name" value="30S ribosomal protein S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023035">
        <property type="entry name" value="Ribosomal_uS9_bac/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_uS9_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001099">
        <property type="entry name" value="PRK00132.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US9M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="318" mass="36373" checksum="9E8466F264DB9020" modified="2011-11-16" version="1">MSLRGLTMIKGLPVSQTRGFMTSCIRLNQAAAEQPQPQQQEQGPRKSFRDQYKKVQTPKLDLSKAREFERLRVVPAYPAFYGGNPIHDANIVKLKEVLAKVSSIPTRRVPHDEIQNERFISFDTYAERCNSGTRVRRRDFDEVLELLNRLRSIDLQLMPQLVIETLNEYRVDSLRALLSQSQRRRKELDEFGRAFGKAKRKNSRAQVYVVKGEGSILVNGKSLNEYFSSEVEREKIVYPFQVIEQMAKYNVFAKVSGGGVTGQSESIMYAISKALIVFNPLLKSRLSKAGLMTSDSRVVERKKPGKVKSRKSPTWVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANT9</accession>
    <name>G3ANT9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Plasma membrane proteolipid 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_61127</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375300.1">
        <property type="nucleotide sequence ID" value="XM_007375238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANT9"/>
    <dbReference type="GeneID" id="18873728"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61127"/>
    <dbReference type="HOGENOM" id="CLU_2484746_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANT9"/>
    <dbReference type="OrthoDB" id="2802411at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659">
        <property type="entry name" value="HYDROPHOBIC PROTEIN RCI2 LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN LTI6 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659:SF42">
        <property type="entry name" value="UPF0057 MEMBRANE PROTEIN ZK632.10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="87" mass="9553" checksum="6646176703724062" modified="2011-11-16" version="1">MDSNDWLLIILAFLIPPIPVIVKKGCSNSVFLNILLCLLGGVFGIIHAVYVVATNPYESPYYDFESYRSSSCSSKRSSSSSLPHPNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP99</accession>
    <name>G3AP99_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_56069</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375946.1">
        <property type="nucleotide sequence ID" value="XM_007375884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP99"/>
    <dbReference type="FunCoup" id="G3AP99">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP99"/>
    <dbReference type="GeneID" id="18871996"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56069"/>
    <dbReference type="eggNOG" id="ENOG502RXPE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073166_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP99"/>
    <dbReference type="OMA" id="FVENECK"/>
    <dbReference type="OrthoDB" id="198787at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021211">
        <property type="entry name" value="SAM35"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10806">
        <property type="entry name" value="SAM35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="289" mass="33049" checksum="102E1F91CF70A9B9" modified="2011-11-16" version="1">MLQVPQTIKTFFDAVPVKNISNDHTKLDDINIYHFQGTSNSQAKFTLAVFNTTQEGDLIIPTDPISLGYTLILSHKSKFTLPKENGSSNCGIMAMSHLGSPNNVLPVLIEDDNLRNIKTLDSINHDLIENNFDSHEAKLVNSLVDSKFYDVWVQCILNEDLPYKVLSELFGLDETIEGNVVLRETQKYEFLTQVANWNSFKLRHPTLFEQWTRTNYLKNYYESQIGEFAKDLQLIIEYLNNNSDEQSTLIKHKVAGYMISIDKFLKSTKLGDVVVSQGEFLHQCYDLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3APA3</accession>
    <name>G3APA3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Probable electron transfer flavoprotein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_56074</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="9 10">Binds 1 FAD per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375950.1">
        <property type="nucleotide sequence ID" value="XM_007375888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APA3"/>
    <dbReference type="FunCoup" id="G3APA3">
        <property type="interactions" value="519"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APA3"/>
    <dbReference type="GeneID" id="18871997"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56074"/>
    <dbReference type="eggNOG" id="KOG3954">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034178_0_1_1"/>
    <dbReference type="InParanoid" id="G3APA3"/>
    <dbReference type="OMA" id="WRPYAEQ"/>
    <dbReference type="OrthoDB" id="1715808at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01715">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000041">
        <property type="entry name" value="Electron transfer flavoprotein alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000001">
        <property type="entry name" value="Electron transfer flavoprotein, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033947">
        <property type="entry name" value="ETF_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153:SF1">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000089">
        <property type="entry name" value="Electra_flavoP_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 9 10" id="KW-0274">FAD</keyword>
    <keyword evidence="5 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="11">
        <location>
            <begin position="20"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="246"/>
            <end position="247"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="260"/>
            <end position="264"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="277"/>
            <end position="284"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000089-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="328" mass="33973" checksum="D5C55BC00AECAF4A" modified="2011-11-16" version="1">MSLSLIARRYFSSSKSALNTLVFIESNASGATTPASLSAITAATQLGKPVTALVVGSNADSIAPQASKAGVSKVLVAKDAKYDHYLAEEVAPLVENIIKESGYTHFITPASSVGKSVLPRVAASLDVQPISDIVKIVNENTFVRPIYAGNALATVKSKDSIILASVRASAFAPVATSGSAAPIEEVSTVDTDNRTEFVSEELVSDGFDLSSSKIVVAGGRGLKNKATFDELMNPLASKLKAAIGASRAAVDSGFCDNSLQIGQTGKIIAPELYIGVGISGAIQHLAGMKDSKTIVAINKDPDAPIFNVADIGLVGDLNEVIPELTKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3APG0</accession>
    <name>G3APG0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-rRNA-processing protein ESF2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">18S rRNA factor 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Pre-rRNA-processing protein esf2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_139224</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESF2/ABP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375413.1">
        <property type="nucleotide sequence ID" value="XM_007375351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APG0"/>
    <dbReference type="FunCoup" id="G3APG0">
        <property type="interactions" value="976"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APG0"/>
    <dbReference type="GeneID" id="18870190"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139224"/>
    <dbReference type="eggNOG" id="KOG3152">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054086_0_0_1"/>
    <dbReference type="InParanoid" id="G3APG0"/>
    <dbReference type="OMA" id="TRKHNDF"/>
    <dbReference type="OrthoDB" id="287393at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034462">
        <property type="term" value="P:small-subunit processome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12263">
        <property type="entry name" value="RRM_ABT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039119">
        <property type="entry name" value="ABT1/Esf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034353">
        <property type="entry name" value="ABT1/ESF2_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311">
        <property type="entry name" value="ACTIVATOR OF BASAL TRANSCRIPTION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311:SF7">
        <property type="entry name" value="ACTIVATOR OF BASAL TRANSCRIPTION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="271"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="53"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="280"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="306"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="329" mass="37906" checksum="B2CC74EBAC2B00A0" modified="2011-11-16" version="1">MGFTKEEQYSSDDEPLDINTVNDSDFDDDEEEDMKSKVFSFKPKVRGSANNLEDLDEPSDNDDGLDEASDDDNKDDSVYDNEEVANVQDSELNVSGGDEPKPKKNKKLKKLTSKELEKEQKRIRKTGVCYLSKIPPYMKPSKLRSVLSRFGAIDRLFLKPEDIAVYHKRVKYGGNKKKNFTEGWVEFVSKSDAKLCAATLNGNKLGGKKTSYYYDDVMNIKYLHGFKWLDLTQQIARENEAREAKLAMELSQQQKLNKSFIHNVEKSKMITNIQRKRSAKQQDSKQEEDEIRRNFKQRKVASTRSAAKEELKEKSKPNEKLNDILSKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3APT1</accession>
    <name>G3APT1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_50820</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375528.1">
        <property type="nucleotide sequence ID" value="XM_007375466.1"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APT1"/>
    <dbReference type="GeneID" id="18871490"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50820"/>
    <dbReference type="eggNOG" id="KOG0588">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003363_1_0_1"/>
    <dbReference type="InParanoid" id="G3APT1"/>
    <dbReference type="OMA" id="KGHNRFS"/>
    <dbReference type="OrthoDB" id="504170at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005940">
        <property type="term" value="C:septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060258">
        <property type="term" value="P:negative regulation of filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001558">
        <property type="term" value="P:regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14081">
        <property type="entry name" value="STKc_BRSK1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000394">
        <property type="entry name" value="Serine/threonine-protein kinase HSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031850">
        <property type="entry name" value="Fungal_KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16797">
        <property type="entry name" value="Fungal_KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="77"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="519"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="634"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="800"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="978"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1158"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1424"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="520"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="565"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="656"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="981"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1002"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="1162"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1427"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1442"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW32252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1451" mass="162945" checksum="E8F0B585C097D385" modified="2011-11-16" version="1">MASVKTQFEDHTFNDTITGTPTTTRTTTDSASQTPTPANVDKVVQSVTNATKRLSQISTITNNSNKKRKSQNKIGPWKLGRTLGRGSTGRVRLAKNINTGKLAAVKIVPKSNFKKLENPKYKRNQDDQDSGLPYGIEREIIIMKLISHPNIMGLYDVWENKNDLYLILEYIEGGELFDYLIKRGKLQEFEAINYFKQIINGINYLHQFNICHRDLKPENLLLDFNKNIKIADFGMAALEIKEKLLETSCGSPHYASPEIVAGKNYHGAPSDIWSCGIILFALLTGHLPFDDENIRKLLLKVQSGKFNMPHDLSWEAKDLISKMLKVNPKDRISIEDILLHPLLTKYPDPPTPSSVTGTTLDLKNMNINPIESIDKIDQEILKNLSTLFHNCNEQTIISRLMSPNKCSEKMFYYLLMKYRNEHLSTSSSMDISDEATDVGSARNSLPRSTSVIKTTVVDDATGEVQTHTMVKKVENSTSIYSNKSLLNKKTLLDNVNHKQHPASNSVNKKKVVHNNQVIYSKSRNASSRSLRSNKSTSSTKNSQPSPSSSESGGRVSTPMVKLPMQSPPNVPLPTTPVRGPNKQIIKPTTIETTIRTQNDKDSMRRRLTGTFGNKSLLNFELICEEVFSGDDNKENMTPALSTKTATPPPKKQAANYKERESSLARRERELAERVRSQNEAREQRLKQAEETERKLQAQQEKRRSLASIAEEQLVEKQRAVFNRLLNRQSTNDFANLVGDRRSVTEPPVHSLDPKVSSLLRARTLATPVPRKFEATGSESPTKKVLNKLGIELDVTESPASNRFKKTGSSRNLTGILKPRDSNLPSRVPSKGSLLKVSTSRNLAGYLKSEANEEEPDEIANIKMTEHSNLSQTSFYKSLDDKRKSSTVPIVEEEGEEEDVISYSQIPETSLPNPRFSTFSFGGLLLSKPQTATNEEGDLTIMQNTLSSGGTVVRRSIYKNSSIRKSSTILGLGIKVRDQEQPEQQQQQSQINQKQVNVSSSSEESKNTRRYSNDDADDELLSDSSTVDTEFSIEESASKQPPPQVIPRFDSGHSIVNSKETLILKKHDSDTGMHEDTTIRLYESYEDLTDKNSSFVAANTTGFDRNFSVPEVTGIIDESVVDDTAFNDNESGLLNARLSTGIFSTSGIPRSPRVELSERYVQEEEEEDDDYVDDEEEDCDDDNELEPVNTFQQREEKRMSVQAEESSKQTKRVSISEKKEFISSTSLQQYDTIVPTTSPGVMIREESPSRVPRYKRMSLYPKRAAPKPPVSEETTTKSNRFSWISVNTEEKTKSNWFKRFFQSLTGGSKKIESTPKHAPKDIEVVDSSLSSDELMQVIKNQLELKKLEGSISTVTIDEEFGMISGVIPAKYSSGRKLKFTIEVITFAQSSSLHIIKIKGNDKGFQNFVNIVTFVIRQEEFMNRKRMSSRGTTATRTSGRSAANNRNLITVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ58</accession>
    <name>G3AQ58_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Quinone oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_154511</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376182.1">
        <property type="nucleotide sequence ID" value="XM_007376120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ58"/>
    <dbReference type="GeneID" id="18870987"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_154511"/>
    <dbReference type="eggNOG" id="KOG1198">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_16_5_1"/>
    <dbReference type="InParanoid" id="G3AQ58"/>
    <dbReference type="OMA" id="IDWKGPA"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08249">
        <property type="entry name" value="enoyl_reductase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047122">
        <property type="entry name" value="Trans-enoyl_RdTase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348">
        <property type="entry name" value="HYPOTHETICAL OXIDOREDUCTASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348:SF2">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C2E1P3.01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="2">
        <location>
            <begin position="58"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="386" mass="42847" checksum="F54175B116559C25" modified="2011-11-16" version="1">MVTYTEVSRKRERTDSDSELDPVTLRKIQKVGSEEPETESKDAPIILSRSQSVLSVTDLKEPLAIQSHPVPEISPNEILVHNRAIGLNPIDWKGKKYGFGIYHFPWINGRESSGKVVHVGSEVKNVKAGDDVIVSSTSYRDNRTSTFQEYTAIDSRLVWKLPKSLTYEEGATIGVGLVTAGIIFYNSFGFQLSEIPEQADGYLLIWGGATVVGIYLTQLAKLYGLKVISIASLDHKDYLVSLGADHVINRHLTEAQIVAKIKSIVGEEGIKYGVDCVSKQTSTIVLDILDQTKSSLSTEPAQFAGIVGVPKEHPESVEIKEVVIKRFHEDLKFGEQFITATSHFLNKRLIKPVRYRHYKGGLHIIDNALKDLENKGAKGEKYVVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ64</accession>
    <name>G3AQ64_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nucleolar complex protein 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_154530</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in the nuclear export of 40S pre-ribosomal subunit to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP14 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376189.1">
        <property type="nucleotide sequence ID" value="XM_007376127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ64"/>
    <dbReference type="FunCoup" id="G3AQ64">
        <property type="interactions" value="1163"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ64"/>
    <dbReference type="GeneID" id="18870988"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_154530"/>
    <dbReference type="eggNOG" id="KOG2147">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008874_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQ64"/>
    <dbReference type="OMA" id="KSCWPSL"/>
    <dbReference type="OrthoDB" id="441771at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030692">
        <property type="term" value="C:Noc4p-Nop14p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007276">
        <property type="entry name" value="Nop14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183">
        <property type="entry name" value="NOP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04147">
        <property type="entry name" value="Nop14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="13"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="135"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="319"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="348"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="787" mass="90931" checksum="247921E6EE1D6081" modified="2011-11-16" version="1">MAGSQLKQLKAALKEKGLIGQTNTKSKKSKHNKAEVKRDDRQHKIDEVRNEFNKFDTKINRTKHDVSIIQQGKFVKMGSKQHNETSARNGAVLKTMKMQYNLEKGRRGKTGGILDKRFGESDKHLSKEEKMLARFTKERQNASRKRNAFSLESDEEDEDDDEDGFTLTHSGQALSLDDEPSVKYVDEDYSVEDEQEQPARKKSKNEVMKEIIAKSKFYKQQRQKEFAKTQDEIDVLDDEFGDVMDDLRNVQTATPVFSTKSAESIEYDSKVRELTYDRRAVPADRTKTQEELIKEHEDKLKKLEADRLRRMEGFVDSREAEADDLDDEFWAGGSDEDEEQGFAIKEDGGDDSESESEDEHSGRPKTVKTPPVIMPYTHHEFEQEVSQIEPAKQANHINKIIQAYKPNLAQGNKEKMNNFVGILFQHVLHLANHDQEFQHIVEIVKKLAETYNEKLVGTIREEISHIQSRILKHHLRKSDLVFFVVIGYLFSTSDHYHLIVTPCLILMNELLTSIIYSPNTNVQTIGQGIFTVDVLLSYQRFAKRFDPEVVNFIEYAMLQLIPEPKKMTGLDKILSSKTESRFSVSKTEQINTGIISIKQLFKEEGELKSQLLVKLVDIMDKLVSLYKEKSCLIEILDSFIPILKHLVKYFAASIPNLSSTLTKFTKLQSNLVKSRKPLTLQHHRALAMATFAPKFEENFNPDKKSYDVNKERADLNKIKYQIKMEKKAALKDIRQENKFVAREQIAEKKSMYDEYHKKMANIVNSISTIEGAEKNQYEREKKLRKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ88</accession>
    <name>G3AQ88_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Altered inheritance of mitochondria protein 23, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_61997</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AIM23 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376213.1">
        <property type="nucleotide sequence ID" value="XM_007376151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ88"/>
    <dbReference type="GeneID" id="18874143"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61997"/>
    <dbReference type="eggNOG" id="ENOG502RY27">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1013961_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQ88"/>
    <dbReference type="OrthoDB" id="3996489at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029427">
        <property type="entry name" value="AIM23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14877">
        <property type="entry name" value="mIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="52"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="52"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="275" mass="32288" checksum="9384DFF68D32900E" modified="2011-11-16" version="1" fragment="single">MLVRQSTYNIIKRTYKNSTICFNLFKDFNHDDNSHKPRQSAGKSQFEKFNRFRTHSESQEIPRRQERWNKSDNKRSNRDNNRPRKFFIHSGTESCQNAIRSIVLRVQEISPDFKVQYLNPETNSIEVLHLADIVNSMDLTKQGITCVELDDNYPLVKLCKVHEMTKVYSDKLAKIKEQELLAMGSKKTAYAIQSREKALSKRSAEKVISCKWSINTSDLLNQKKNEILKRLSKGENLQVRFQTNSNRGFDTDSSHVSHMHRNEDDYNLELKKREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQE6</accession>
    <name>G3AQE6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_140011</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376271.1">
        <property type="nucleotide sequence ID" value="XM_007376209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQE6"/>
    <dbReference type="STRING" id="619300.G3AQE6"/>
    <dbReference type="GeneID" id="18870263"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140011"/>
    <dbReference type="eggNOG" id="ENOG502TBA8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036640_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQE6"/>
    <dbReference type="OMA" id="DFFESYM"/>
    <dbReference type="OrthoDB" id="4091783at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase III" evidence="1">
        <location>
            <begin position="226"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="428" mass="49978" checksum="814F4D9BDCE538F3" modified="2011-11-16" version="1">MNNYRPHVSYNNPTILIPLRLDELLRSDNSKKSHFISLGEDFVRLSSYQRFRELLSFGGGTLIKTVVFDLSTFLKREQNNTDSHTYAEFADFLTSNDKMFEREGGMTPSRRSIGLKNWVFMIIGYLLHSYSEKEAEKCLDQLIKIYLNSRNDNLFTASNHTTKDLTDARDKFMLDTFNQYVKKVPIRYKLLFGRNPYRHVSFHEGLDYKKVSLPELPRLRNRELLVKALMHKEFYRILLDPRHEFAQTMVAAGYDLNHKEYSIVRKQVSFLDGLGDLFLAHETSKVIYELYSDGLPLNNGSYRLLRTMLATNTFMTKLAIAYNLHVGLDDPIINERVANEWIPCIMFGKEMEKSNRVYEEEFLGDFFEAYVAALLIEQPDVAKSFIKDIYSRLLEVITETLPPELSYHNWSTNVLGRSIYPKSERVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQQ3</accession>
    <name>G3AQQ3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">40S ribosomal protein S15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_51594</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376378.1">
        <property type="nucleotide sequence ID" value="XM_007376316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQQ3"/>
    <dbReference type="FunCoup" id="G3AQQ3">
        <property type="interactions" value="863"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQQ3"/>
    <dbReference type="GeneID" id="18871575"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51594"/>
    <dbReference type="eggNOG" id="KOG0898">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097347_1_0_1"/>
    <dbReference type="InParanoid" id="G3AQQ3"/>
    <dbReference type="OMA" id="KTHCRDM"/>
    <dbReference type="OrthoDB" id="10258210at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.860.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="entry name" value="30s Ribosomal Protein S19, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_uS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_uS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_uS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005713">
        <property type="entry name" value="Ribosomal_uS19_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_uS19_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003121">
        <property type="entry name" value="PRK04038.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01025">
        <property type="entry name" value="uS19_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="RIBOSOMAL PROTEIN S19P FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="Ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW31600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="142" mass="16158" checksum="CB196A1B973FA9BA" modified="2011-11-16" version="1">MSDAVERKKRNFKTFTFKGVDLKDLVEMPTEEFTKLCSARVRRRFSRGLDSKPMGLIKKLRIARANTQPNEKPATVKTHLRNMVIVPEMIGSVVGVYNGKVFNTVEIKPEMVGHYLGEFSITYTPVRHGRAGNASSRFMPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQV2</accession>
    <name>G3AQV2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nuclear control of ATPase protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_56454</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376427.1">
        <property type="nucleotide sequence ID" value="XM_007376365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQV2"/>
    <dbReference type="FunCoup" id="G3AQV2">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQV2"/>
    <dbReference type="GeneID" id="18872034"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56454"/>
    <dbReference type="eggNOG" id="ENOG502QTT6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008227_2_0_1"/>
    <dbReference type="InParanoid" id="G3AQV2"/>
    <dbReference type="OMA" id="NRIYHVY"/>
    <dbReference type="OrthoDB" id="413313at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013946">
        <property type="entry name" value="NCA2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28234">
        <property type="entry name" value="NUCLEAR CONTROL OF ATPASE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28234:SF1">
        <property type="entry name" value="NUCLEAR CONTROL OF ATPASE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08637">
        <property type="entry name" value="NCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW31649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="618" mass="70834" checksum="98B9D6F89F980BA3" modified="2011-11-16" version="1">MEFLSLGLSPGSRKSIKVIKEINNNNRISLSIKQKVNDLNLLTKSFVSEQLSQYDEATISLAKQDSASFNAIQEAFGELYFLLDCENFKFEKFSTVGLPKVKQIYQKFANYDSSAPGVSTQLNQVNGTAVELVNNYMVLLSTLNLSSSLIYKTLVLKNQQVYWESIHSSTYNKLIYFIQISPVRLFKFVRIYGTWTLIKYYTTALYRVMGRSVNAMFVKEVPSVSFLNLSKSARSLTWYKNAFKFIIRFPIITANNDIKTKLKIINQEIENNTASIDALLKTNLNDKQSTLNSLESILKLEGKDNEFAKVSGIIDSIIELDNKRYNETDKPSFITRYWVVFAILLRYGPFELLNAYNNRLEIMDWINENGIQPIIGFFKNWVIKPMTELVNILKRDDEITITTKDSLKSDFSSLERMIVEFAQDNKLDTTPEIIKQDVANGDLSLIMTRYENEIRNPIRYTLSGSLLRLLLIQIQKGKVDGGVAVNGIDKVLKSQQLVFGFVSMSPSLFILYQLYKYITSPVPITVNGKQLNIVCLKSLNNIENLLVLLSQSDGKDQFEGELLIEIINLIVSSDLIIPKQLHEDWVRDLNELNNIDFDIATKLNLVHKIWNMYGPYFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR43</accession>
    <name>G3AR43_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GST C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62309</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376482.1">
        <property type="nucleotide sequence ID" value="XM_007376420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR43"/>
    <dbReference type="STRING" id="619300.G3AR43"/>
    <dbReference type="GeneID" id="18874264"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62309"/>
    <dbReference type="eggNOG" id="KOG0867">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2661404_0_0_1"/>
    <dbReference type="InParanoid" id="G3AR43"/>
    <dbReference type="OrthoDB" id="249703at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050802">
        <property type="entry name" value="EF-GSTs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="76" mass="9272" checksum="69FB54015C82E4F9" modified="2011-11-16" version="1" fragment="single">MIPYRKKDVEEARVYLERVVAVFEARLANYTYLVSERITFGDIFSATFFKRGFDWCFGAKWRKQHPNVTRWWKTIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR89</accession>
    <name>G3AR89_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BRO domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61836</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376042.1">
        <property type="nucleotide sequence ID" value="XM_007375980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR89"/>
    <dbReference type="STRING" id="619300.G3AR89"/>
    <dbReference type="GeneID" id="18874075"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61836"/>
    <dbReference type="eggNOG" id="KOG2220">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1786568_0_0_1"/>
    <dbReference type="InParanoid" id="G3AR89"/>
    <dbReference type="OMA" id="KLEFEWF"/>
    <dbReference type="OrthoDB" id="2141925at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030">
        <property type="entry name" value="PCD6 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030:SF30">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="2">
        <location>
            <begin position="4"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00041284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="171" mass="19530" checksum="C2E2D2D10E67C613" modified="2011-11-16" version="1" fragment="single">MKTHLLTVPSKKTEEINWVKPLNNYLVSIYGNTSSYQDDLNSFNKLRQDIRGVNADNTGLKLYYKYYSQLELLDLRIPFATVNKHKKLEFEWFDAFSPTVANKQAALPFEKANVLFNLAALLTRFAKHKYDESIQDSGSEGVDDATKSTIQLLQSAAGVYQFINENFLHAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARA4</accession>
    <name>G3ARA4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">DNA polymerase</fullName>
            <ecNumber evidence="20">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">SPAPADRAFT_72459</name>
    </gene>
    <organism evidence="27">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18 20">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="19">Forms DNA polymerase zeta with REV7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 20">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="20"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376489.1">
        <property type="nucleotide sequence ID" value="XM_007376427.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3ARA4">
        <property type="interactions" value="684"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARA4"/>
    <dbReference type="GeneID" id="18875436"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72459"/>
    <dbReference type="eggNOG" id="KOG0968">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000203_3_1_1"/>
    <dbReference type="InParanoid" id="G3ARA4"/>
    <dbReference type="OMA" id="GRNKMGF"/>
    <dbReference type="OrthoDB" id="2414538at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016035">
        <property type="term" value="C:zeta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05778">
        <property type="entry name" value="DNA_polB_zeta_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05534">
        <property type="entry name" value="POLBc_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000007">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000002">
        <property type="entry name" value="DNA polymerase zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056435">
        <property type="entry name" value="DPOD/Z_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030559">
        <property type="entry name" value="PolZ_Rev3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025687">
        <property type="entry name" value="Znf-C4pol"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812:SF1">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24055">
        <property type="entry name" value="POL3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14260">
        <property type="entry name" value="zf-C4pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 20" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 20" id="KW-0235">DNA replication</keyword>
    <keyword evidence="15 20" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12 20" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="13 20" id="KW-0408">Iron</keyword>
    <keyword evidence="14 20" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 20" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17 20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 20" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase delta/zeta catalytic subunit N-terminal" evidence="25">
        <location>
            <begin position="148"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="23">
        <location>
            <begin position="667"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="22">
        <location>
            <begin position="959"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="domain" description="C4-type zinc-finger of DNA polymerase delta" evidence="24">
        <location>
            <begin position="1402"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00066055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF14260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF24055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="EGW31711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1519" mass="175821" checksum="CBF0898DFA04F78F" modified="2011-11-16" version="1">MRSLDSLRVQINDYDTYQSYPTRLDQYYSKVLQVPIIRIYGSITVDENIISRNYLSPTKKKHKHKSDTPSYVFNVVIHVHNFYPYLYVDCRDQDYAKLSQRSHINKVIDHLEEALKISYANGRYRKKNEEDLSEDDDDQYEAPSTTSYKQSSTKRRYIANVSICKAVPMYGFQIGYNLFYKISLLSPNYKPRLTKLFQENKLSLHNIGREDNNKTRNYDAHYVFEAHIPHLLQFLTDFNLFGCGWLYIDKDHIRDGSNTKSLYFRSPIVNEALKSAYTKTQFDVLLRYLTNFVTHDNVLKGNDLDNEGIGPFTRIGKSVLEIDISTSGITNRTWLSPRQLHDNFTERKEYDEYNASIQDGKNPQHGYKYDEFGNPRIYLSSLKQIYNDLKYQCDIRNSSLELYSDVLATTNELSYFGTGSTNWSNQQRLNELLEYAIQLTGSNELDMEKYADTYLTNLKSRKINGNDMFDKFKTSFDLVDYEKELQYHNIPMRPGIDINNISHSSSSIEDITFTPSLEGTNHMGLNIGDNCYEVVIPDSVKPENINNTFQKAGKLKINYQDPFYDNLNDIPTKPFVFANKKIVVPLKDSSSIPMTSSTKMIHDNLQDDTETRKCIRSRKFYTWQYLQDPPSKQNVQTWAKLEEEKLQRRALKYGSQDEPGITQSYDFKFSYNSMKIARRPNGYNSLTCFSMEIHADPPNSKLTVDPLRDPISIVFYSFDDKNGMFNHFEYTSGILIFNTRKYSEQFISQLSSSLKKNIELFDDEVEMTARLVQLIEIFDPDILAGYEINAMSWGYIIERLRNVFDINILADFSRGSDKSNGKFGDRWGYTHTSNIEINGRHMFNVWRLLRSDLSLTSYSLENVSYHLLHRSLPRYSNYQLSQWLSTGTFSNVLVALSYYSTRIDMVIKIIEVQELITRNVEHSRLIGIEFNANFYRGSQFKVESILARLTKSESLLLNSPSKQQKSPLVVLDFQSLYPSIMIAYNYCYSTLLGKLNNFSPNMNDIGYLDNLKIPPGMIDLLKKDNAINISPNGFIFVKSNVRKSILAKMLEEFLALRIKVKAVTKSFRDDTELTRVYNSRQLALKLIANVTYGYTSATFSGRMPNSDISDAIVSTGREILMKSIELIEAGNYGAKVVYGDTDSLFVYFPGKSKDEAFKLGKSIAQYITSVFPDPVKLKFEKVYHPCVLLTKKRYVGYAYEYEDQKVPKFDAKGIETVRRDGIPAQSKMTEKALRILFETQNLSKVKEYTLKQFYKISINKISVQDFCFAKEVRYGTYKNEKYLPPGAVVANKMVEDDPRKEPQYRERVPYVIIQDATKHRIKDRCISPEDLIASYKTDKPLMLDYEYYITRVLIPPLARVFNLLGVDVKGWYRELPSFSQSRSRLLRNDGLLQSGVLNSHTCLSCHEKLDLNNSMVCSSCTSDEQQLVATICSECRENELRVRKLEKLCKDCVYSAFGNKGNNITNQSAENCSNKDCSVYYKKFRSQNDRDLSKFPGTYVQHRPLDHQSLLHPSIQCRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARG2</accession>
    <name>G3ARG2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_61849</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376061.1">
        <property type="nucleotide sequence ID" value="XM_007375999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARG2"/>
    <dbReference type="FunCoup" id="G3ARG2">
        <property type="interactions" value="1210"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARG2"/>
    <dbReference type="GeneID" id="18874082"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61849"/>
    <dbReference type="eggNOG" id="KOG3320">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088621_1_2_1"/>
    <dbReference type="InParanoid" id="G3ARG2"/>
    <dbReference type="OMA" id="AAYHKVQ"/>
    <dbReference type="OrthoDB" id="1724687at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000554">
        <property type="entry name" value="Ribosomal_eS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047861">
        <property type="entry name" value="Ribosomal_eS7_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11278">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11278:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01251">
        <property type="entry name" value="Ribosomal_S7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00948">
        <property type="entry name" value="RIBOSOMAL_S7E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW31283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="188" mass="21468" checksum="9469BCB3CAB3BD3F" modified="2011-11-16" version="1">MSAVNKILAEKPTELELKVAQAFVDLEAQADLKADLRPLQFKSIKEIDVNGGKKALAVFVPPPSLQAYRKVQTRLTRELEKKFPDRHVVFLAERRILPKPSRKARKQQKRPRSRTLTAVHDKILEDLVFPTEIIGKRVRYLVGGNKIQKVLLDSKDSTAVDYKLDSFQQLYSKLTGKQVVFEIPGETY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3ART0</accession>
    <name>G3ART0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Altered inheritance of mitochondria protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">AIM11</name>
        <name evidence="8" type="ORF">SPAPADRAFT_140637</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the AIM11 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376125.1">
        <property type="nucleotide sequence ID" value="XM_007376063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ART0"/>
    <dbReference type="FunCoup" id="G3ART0">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="GeneID" id="18870310"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140637"/>
    <dbReference type="eggNOG" id="ENOG502SAK0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118700_0_0_1"/>
    <dbReference type="InParanoid" id="G3ART0"/>
    <dbReference type="OMA" id="RFAYKST"/>
    <dbReference type="OrthoDB" id="4088121at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038814">
        <property type="entry name" value="AIM11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39136">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39136:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="161" mass="18194" checksum="6D030EC006E7D63C" modified="2011-11-16" version="1">MTDLLRTLNFKLADASDEYKQRRKIQMMRFMASAAITIFASRFAYKATITRQYVPTLFQGNHSPPLSYNFATDAAVAVGAGTLLCGSVSGMLIFGGFWCLDISNVKEFGWRMKSLMGGDQKEKELSEMPMDEESSYIQDSLNDMLEGKYDFDKIDDETDKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARZ9</accession>
    <name>G3ARZ9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_62459</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376625.1">
        <property type="nucleotide sequence ID" value="XM_007376563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARZ9"/>
    <dbReference type="FunCoup" id="G3ARZ9">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARZ9"/>
    <dbReference type="GeneID" id="18874327"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62459"/>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076142_0_1_1"/>
    <dbReference type="InParanoid" id="G3ARZ9"/>
    <dbReference type="OMA" id="DWDDNYG"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF3">
        <property type="entry name" value="RING ZINC FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="116"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="187"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="200" mass="22575" checksum="9F58627C06612B0C" modified="2011-11-16" version="1">MSTYEEEHNIQDRANPRADDTTARGRQHATLSNIIDTFLTTSGSDTPSNAYAMEALTSALHHLSDADGSRLAQTLMESLQDSGDIDGKRSKGVSQEYLDTLERVKVSSISEDDAFCPICTNKFKDDKYPLVVRLPCGHGVNHIYDLECVGPWLQMNSTCPLCRTNVLEAEQNRRKKIEEEIRRAKEEDSEEEEEDWDVYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS27</accession>
    <name>G3AS27_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_140899</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376655.1">
        <property type="nucleotide sequence ID" value="XM_007376593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS27"/>
    <dbReference type="FunCoup" id="G3AS27">
        <property type="interactions" value="344"/>
    </dbReference>
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    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140899"/>
    <dbReference type="eggNOG" id="ENOG502QPKX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_796925_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS27"/>
    <dbReference type="OMA" id="TTAHEYK"/>
    <dbReference type="OrthoDB" id="4081967at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035189">
        <property type="entry name" value="Std1/Mth1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17235">
        <property type="entry name" value="STD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="293"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="347" mass="38432" checksum="C009F9F798526252" modified="2011-11-16" version="1">MYGSPFSNNNRSKTSTPSHFRDSARNAIYQQLPPSVITSPPANGGAGGGGGASGGAASIRSAPPLNHNSKYQMSHRSSSSSIFSSISGKRSVRSAPSIYSSSTATIPEDSEPISTTVEQLVNDIALHESHFQQQQPSEKPVVGDDLDPEEMYKISLGSDLQYQNVSESLIDWNLNVTRCKLIITQLPVISTIPDFQYNQNALPQLIGDLASSCHIVLIQPHISDKELIYTLFTSNLYQEHNLDTSFKKSVAEISVKQSRLLQINSGKGISQHQHSFLKFKFKEIAIRNYLINLAAAATTAHEYKLRLDQLKMELRTIEGKKVKLSKEDKKQLWEKVRSDVFQRAGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS95</accession>
    <name>G3AS95_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CTP synthase</fullName>
            <ecNumber evidence="9">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_62950</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CTP synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="9"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377087.1">
        <property type="nucleotide sequence ID" value="XM_007377025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS95"/>
    <dbReference type="FunCoup" id="G3AS95">
        <property type="interactions" value="950"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AS95"/>
    <dbReference type="GeneID" id="18874535"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62950"/>
    <dbReference type="eggNOG" id="KOG2387">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011675_5_0_1"/>
    <dbReference type="InParanoid" id="G3AS95"/>
    <dbReference type="OMA" id="EFNNAYR"/>
    <dbReference type="OrthoDB" id="1739076at2759"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097268">
        <property type="term" value="C:cytoophidium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000207">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000005">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003792">
        <property type="entry name" value="PRK05380.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550:SF0">
        <property type="entry name" value="CTP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP synthase N-terminal" evidence="11">
        <location>
            <begin position="2"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase" evidence="10">
        <location>
            <begin position="313"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU810713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="573" mass="63927" checksum="C90E27654C17BA91" modified="2011-11-16" version="1">MKYVVVSGGVISGIGKGVIASSTGLLFKTLGLRVTSIKIDPYMNIDAGTMSPLEHGECFVLNDGGEVDLDLGNYERYLNITLTRNHNITTGKIYSQVIEKERKGDYLGKTVQVVPHLTNAIQEWIERVAKIPVDDTGLEPEVCIIELGGTVGDIESAPFVEALRQFQFRVGTENFALIHVSLVPVIHGEQKTKPTQAAIKDLRSLGLTPDMIACRCQDVLEVATIEKIGMFCHVGPQQVIAVHDVNSTYHVPLLLKQQKMMKYLTKKLAIGDIDATALARGEELLTRWKHLTTSHDKSFETVTIALVGKYTHLKDSYLSVIKSLEHASMRCNRRLTIEWVESTNLEEETKRTNLSEYHKAWHYVCQADGILVPGGFGNRGIEGMIAAAKYARENDIPYLGVCLGLQIAVIEFARNVLGKTGTTSMEFDPSLSEEEATVVYMPDVDQVKLGGTMRLGIHPTKFVADTKWSKLRKLYGGADAVYERHRHRYEVNPLLIDEIEAKGLKFIGKDETEKRMEMIELKDHKFFTGTQYHPEYLSKVLDPSRPFLGLVAAASGILEAVLERDDLNYKGDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASQ2</accession>
    <name>G3ASQ2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Crh-like protein</fullName>
            <ecNumber evidence="16">3.2.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">SPAPADRAFT_142833</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <topology evidence="3">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the glycosyl hydrolase 16 family. CRH1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.2.-.-" evidence="16"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377149.1">
        <property type="nucleotide sequence ID" value="XM_007377087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASQ2"/>
    <dbReference type="STRING" id="619300.G3ASQ2"/>
    <dbReference type="GeneID" id="18870491"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142833"/>
    <dbReference type="eggNOG" id="ENOG502QQ71">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027506_2_0_1"/>
    <dbReference type="InParanoid" id="G3ASQ2"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02183">
        <property type="entry name" value="GH16_fungal_CRH1_transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017168">
        <property type="entry name" value="CHR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF68">
        <property type="entry name" value="GLYCOSIDASE CRH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037299">
        <property type="entry name" value="Glycosidase_CRH1_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="10 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442593" description="Crh-like protein" evidence="20">
        <location>
            <begin position="20"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="21">
        <location>
            <begin position="24"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="283"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="17">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="17">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="36"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR037299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR037299-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR037299-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="EGW31116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="434" mass="48619" checksum="5E4E330ACA27276B" modified="2011-11-16" version="1">MIKLNFLLVFILSILSVTSLDSDDDSDDSDLELERCNPLTDNQCLVTNEALGTSIFESFQTGTRYFAITSSRNRIMFDPNGLQLTIKKEFDNPSLVSSFYIMYGKVEAEIKGAHGRGIISSFYLQSDDLDEIDIVEVFGGDPYEYQTNYFVKGNTTNYDRGRYHSLATSPLTEFHKYGMEWTPHKITWFLDGKKVRVLNKKNKYGFPCSPSFLKFSLWAGGDYSNDEGTITWAGGPTNYDEGPFTMTIRNLNVTDYSNGVAYSYGNLSGGKWLDLQANGGEIYDEEDEDPESPTKTSTIPKTKTKTKVPKSQLLEKTTRKKTTVATNNLYQLPKVTEFKSDGAVPASTTKTKALYTTTVSITELLDQDVVHSSRPSAYSTSWYNTLPNTNNSTSEYKFVQDVTEEEEDLISGVSQIDSIPIAVIVVIISVVTWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AST2</accession>
    <name>G3AST2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_142378</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377179.1">
        <property type="nucleotide sequence ID" value="XM_007377117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AST2"/>
    <dbReference type="GeneID" id="18870443"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142378"/>
    <dbReference type="eggNOG" id="KOG3662">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011607_0_1_1"/>
    <dbReference type="InParanoid" id="G3AST2"/>
    <dbReference type="OMA" id="GPYRGHW"/>
    <dbReference type="OrthoDB" id="5977743at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033308">
        <property type="entry name" value="PGAP5/Cdc1/Ted1"/>
    </dbReference>
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        <property type="entry name" value="METALLO PHOSPHOESTERASE RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315:SF4">
        <property type="entry name" value="METALLOPHOSPHOESTERASE, ISOFORM E"/>
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    </dbReference>
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        <property type="entry name" value="Metallophos"/>
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    </dbReference>
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        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="477"/>
            <end position="500"/>
        </location>
    </feature>
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        <location>
            <begin position="92"/>
            <end position="290"/>
        </location>
    </feature>
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        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EGW31146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="504" mass="58057" checksum="25D4FFC90AA8C7A1" modified="2011-11-16" version="1" fragment="single">IMLFMPKFKSIHQLSLVLLIVWTATFYYHERYTPSHVIRKCQWPSIISEQDVEPPKTSRMLLVADPQLIDSHTYPGRNNLLLKLSQHTVDAYIKKNYHALLSELSPDYVVFLGDLLDNGRESTDTYFYTQLRRFNKIFYDPYKSVYQKGINWFVNLPGNHDIGFGDGVNKYSRERFNREFAPSNIIITLDGVDFVMLDTPSISATSDISYPATQFLQEISSQEKKHPRVLLSHVPLFRDPQLSCGIHREAGPFDVFGHGFQYQNTVSEELTNQILDQVKPDMILSGDDHDHCDIQHIDGTREITVKSISLAMGKKYPAVQLLSFASVGATEEIVYNTDICYMPVPYVNIIHYVIMSVISALLVLWWNLKQKSTRFTYSSSLLPLNSVINIPVESHSRRLKKFLKEQEQDSLPNEQTPPLSPSSSTYTLASGQHLFSIPNYTFTQNNSSSFMKYVKNSKVGQTYIMNRRRVFTFMKRWNLFSFLKNSMMLGISVIGVYYIFCLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASV7</accession>
    <name>G3ASV7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_62597</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASV7"/>
    <dbReference type="GeneID" id="18874395"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62597"/>
    <dbReference type="HOGENOM" id="CLU_1556231_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASV7"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442344" evidence="2">
        <location>
            <begin position="23"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="104"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="205" mass="20827" checksum="52A8C177BA307E11" modified="2011-11-16" version="1">MVRQSLHFCIIFLLLVIQFVNSFPTIYRKSDIADTGALQLNKFYLAKRRSHGSSHSSSSHSSSSHSSSSHSSSSHSSSSHSSSSHSSSSHGSTSGSFSGGSSSGSGSSIRKPKGTTGDDSDSSTTSPKGSTTGTSSGWTSSISSNTYSCGTIGGARRCGYGPYYAPTAAAAAGYGTSRFSNSSSSAAAISVPYVKIAVAVGFALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASY8</accession>
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    </protein>
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    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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        <lineage>
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            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007376742.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASY8"/>
    <dbReference type="FunCoup" id="G3ASY8">
        <property type="interactions" value="666"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASY8"/>
    <dbReference type="GeneID" id="18870470"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142674"/>
    <dbReference type="eggNOG" id="KOG2830">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041824_2_0_1"/>
    <dbReference type="InParanoid" id="G3ASY8"/>
    <dbReference type="OMA" id="EYELCEA"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051721">
        <property type="term" value="F:protein phosphatase 2A binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035303">
        <property type="term" value="P:regulation of dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.540">
        <property type="entry name" value="TAP42-like family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038511">
        <property type="entry name" value="TAP42/TAP46-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007304">
        <property type="entry name" value="TAP46-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933:SF9">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TAP42"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
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            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="347" mass="39904" checksum="0F94133304C2114F" modified="2011-11-16" version="1">MEQLTVGQRYRQAIKAYSQLTNATPVNQSSLVTLIQEFQLISNLIHQLGLFTDNEQLSELNTSYITFLNVDYYLGTLNALVMENRSTILKLSNSQLIQFLKQLSNYQILDKNQQNKLDLFKKSPDNVINNHSSISREEKISNYKAERELSSKLEVLDRYYNDDNDEIEHFDEEIVRQVFVDQLKLHALKAYSLIVSNALELKVLESKPNYVPPPPPPPAQDKREPTRENDYGFTTKLETLPTTATSISQLVSRSGKILQPFTITSERQRLKDKVFGTGQVLPSMSVEEYLDYELSHGKLMKDEVKDKPQDEDYESEEDDEAQLEKRRWDDWKDDNPKGAGNMGGNIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT18</accession>
    <name>G3AT18_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_68371</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377211.1">
        <property type="nucleotide sequence ID" value="XM_007377149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT18"/>
    <dbReference type="STRING" id="619300.G3AT18"/>
    <dbReference type="GeneID" id="18875213"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68371"/>
    <dbReference type="eggNOG" id="KOG1216">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_364918_0_0_1"/>
    <dbReference type="InParanoid" id="G3AT18"/>
    <dbReference type="OMA" id="CEECTEY"/>
    <dbReference type="OrthoDB" id="4022214at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012994462" description="Hyphally-regulated cell wall protein N-terminal domain-containing protein" evidence="7">
        <location>
            <begin position="16"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="8">
        <location>
            <begin position="8"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="407"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="440"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="765" mass="83103" checksum="339F5C0F39C563F6" modified="2011-11-16" version="1">MLSIWLITVVSAILAVVVEDNLFLNPENHVYNEAGDTIITESGKYVITECEDISLQGNFENNGMFLASTKESANGLQFSVSDGQFKNTGTFTLKAEGNTGATSYAIRVNKFVNTGAIIFDTSGSSAPLSQISAGEWENRGLIEVNNVSPSQGQYEHSGFGRNSGIIKLYNQRFTQSSDIYGNGCLYLEEGTECHLQSFYATGSQHFIMGGNSILKVGGSNSGMIYLVSMFGNGKRICLDYPLQTGNGATYDANYGSLVLRSSDVVEEFLIGAGYNQNLFMIEGNCVSYVSEVPQGANGNGFCKIYPSDDPTEANGIMGQGVIANAVDEDAEEPTFYTTTFYDDDGAPVTAEVIVSTNEEQQWYTSTHTLAFTSYTTEVDDGDGEIHTFMVIVTVDDDGIWYTQSDMVQEQGESTKAEDSSSEIEPSSEIEPSSEIEPSSEIEPSSDVVEPSSDIVEPSSELPVVSPSKGPHTEYEEPEPVSYTTTFTDAYGNIITGVVYITTYEDGEWYTSTSIIHRDEDDPGEYTTTKTSVITTSSRGSLYTSTRTTVVIVTSSSQGSASTSTDVLTLTSYTTTYMEDDGLKTGIVIISTTVGGTLYTTTSELELQQHTTTYTDDEGRVRTALVIVSTDTQGNLQTTTSDFEAQKLTITYVDDEGNTKTAEVIVTTDSEGKWHTTVSDFELTKYTTTYTDSAGNTKTAEVHVTTDHEGRWYTTTSEIARKTSTSETPTPSLLAIQDVAEDYATVPKIKLPALLLGVFTVWFLIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATA2</accession>
    <name>G3ATA2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62764</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376898.1">
        <property type="nucleotide sequence ID" value="XM_007376836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATA2"/>
    <dbReference type="GeneID" id="18874456"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62764"/>
    <dbReference type="HOGENOM" id="CLU_2591315_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATA2"/>
    <dbReference type="OrthoDB" id="10295815at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="80" mass="9177" checksum="386BDFC58194EA8D" modified="2011-11-16" version="1">MNYSTKFPSKSLFVFLAPVLYLTSEFVISRFSKSRVTTGSKKLTSSQGSTTAFFDTPINHHTYRTGWIQDHFYNTSNVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATD2</accession>
    <name>G3ATD2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HIG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_62804</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376928.1">
        <property type="nucleotide sequence ID" value="XM_007376866.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATD2"/>
    <dbReference type="FunCoup" id="G3ATD2">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATD2"/>
    <dbReference type="GeneID" id="18874471"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62804"/>
    <dbReference type="eggNOG" id="ENOG502QT50">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079101_3_0_1"/>
    <dbReference type="InParanoid" id="G3ATD2"/>
    <dbReference type="OMA" id="LWMDMVE"/>
    <dbReference type="OrthoDB" id="1915122at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007667">
        <property type="entry name" value="Hypoxia_induced_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040153">
        <property type="entry name" value="Rcf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28018">
        <property type="entry name" value="RESPIRATORY SUPERCOMPLEX FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28018:SF3">
        <property type="entry name" value="RESPIRATORY SUPERCOMPLEX FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04588">
        <property type="entry name" value="HIG_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51503">
        <property type="entry name" value="HIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="HIG1" evidence="6">
        <location>
            <begin position="88"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="216" mass="24998" checksum="A6C41525AF34C26C" modified="2011-11-16" version="1">MKLLTTEEKEAHTSHVIAEGTKGLVYGALVSAGIFTYLKKRHPIKYARFNPSIKTCIFIMPTISIAAFWADQGSWEFDQKMHQSDYHEAKMMEEYREWNRLSLANKIFTVLSNNKYQIIIGAWAASMYGSWVFVNRDKIMSTTQKAVQARMYAQGITVILLLSTILLAMKEQEIESAKPPPIPRWKRIIMEKEAEEKQILREYEEKKKQEEAASNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATL6</accession>
    <name>G3ATL6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Karyopherin functions in nuclear transport of protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_52168</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377012.1">
        <property type="nucleotide sequence ID" value="XM_007376950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATL6"/>
    <dbReference type="FunCoup" id="G3ATL6">
        <property type="interactions" value="1154"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATL6"/>
    <dbReference type="GeneID" id="18871634"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52168"/>
    <dbReference type="eggNOG" id="KOG2171">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003794_0_1_1"/>
    <dbReference type="InParanoid" id="G3ATL6"/>
    <dbReference type="OMA" id="PKRFVQE"/>
    <dbReference type="OrthoDB" id="543373at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041653">
        <property type="entry name" value="Importin_rep_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040928">
        <property type="entry name" value="Importin_rep_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041389">
        <property type="entry name" value="Importin_rep_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057672">
        <property type="entry name" value="TPR_IPO4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18808">
        <property type="entry name" value="Importin_rep_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18816">
        <property type="entry name" value="Importin_rep_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18829">
        <property type="entry name" value="Importin_rep_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25780">
        <property type="entry name" value="TPR_IPO5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="HEAT" evidence="8">
        <location>
            <begin position="142"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="10">
        <location>
            <begin position="353"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="330"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1091" mass="120411" checksum="503B2C8A615CF76D" modified="2011-11-16" version="1">MSLLPEDVQAVLAQLLENLSSADNAIRSAAEKSLDNEWTIQANVEMLLVFLAEQSCMGSNDTIRSFAAVLFRRIAIKSPRELSSVTDRTIGVTSEPVRAQIRSILLKGFTSQQSNQVRHKLSDAISEVAKEDASPAGSWNELIPTLFEATRNPDPSFRESAFRVFSATPELIDKSYLNDVLPVFNSGFEDQTDDVRIAACTAFVAFFRELPKKNWQSLSPLLPNLLNSLPRFLQNGQDQALALVLESLIDLVELAPKMFKDMFPTIIDFCAAVSKNKDLESNTRMASLELLTTFAEVSPSMCKRTPSYTEQMVLITLSMLTEVCIDDDDAAEWNNNDDTEDEDEEPEHDAARQALDRTALRLNGQALAAPLFQYLPAMIQSANWRERQAALMALSSAAEGCADVLMNEIPKILDLILPTLNDDHPRVQYACCNALGQMSTDFADVIQRTAGNKILPALISKLTNKSVPRVQSHAAAALVNFSEAASKEILEPYLDDLLSNLLGLLQSPKRYVQEQVLTTIAIIADAAEKTFIKYYDTLMPLLTDVLKTDMGDENRLLKAKCIECATLIALAVGKEKFAPHCQDLIQLFGHIQETATQDDDPVKQYLEQAWGRICRIIGKDFIPYLPSVLPPLLTSAKATQDISLLEEEQAEEFNSNEEWDVINLSGKLIAVHTAALDEKVAAIDLLRTYAIQLKGDFFPWVKEIIEDIAIPGLDFYLHDGVRGSAALTLASLLKCSVVATGNNSTETLVFWSKISEKLVEVLTNEPVPELLVAYYTALVECITVLGPNSLSSPQLDSLAKSINTNLVEICERIKARDNEDDEYTEDVEEDEDEYTDEELLDEINKALSSIFKNSQANFLPHFQILIPTVASFINDENTNIKLCGLCVVCDILEHCGPNSVIYRDMFANVVAESITSPHASIRQASSYAVGVAAQFGGEDYAQFCLACLEPMFKMASVPDARAEENIHATENSVSAIAKICHRFSSTIPNLNTIIDQWITLLPIVQDESAAPFAYMFLSELIENNHPSINNQIPKVVDSVIQALAHASIAGATAERVVAATRQLLGSVPHSEAIALLQKNPADIDIVQKYFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATV1</accession>
    <name>G3ATV1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA topoisomerase I</fullName>
            <ecNumber evidence="7">5.6.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">DNA topoisomerase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_142998</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6 7">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the type IB topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="7"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377298.1">
        <property type="nucleotide sequence ID" value="XM_007377236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATV1"/>
    <dbReference type="FunCoup" id="G3ATV1">
        <property type="interactions" value="783"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATV1"/>
    <dbReference type="GeneID" id="18870508"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142998"/>
    <dbReference type="eggNOG" id="KOG0981">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009193_1_1_1"/>
    <dbReference type="InParanoid" id="G3ATV1"/>
    <dbReference type="OMA" id="HRWKEVK"/>
    <dbReference type="OrthoDB" id="47179at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000019">
        <property type="term" value="P:regulation of mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009303">
        <property type="term" value="P:rRNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00659">
        <property type="entry name" value="Topo_IB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03488">
        <property type="entry name" value="Topoisomer_IB_N_htopoI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.41:FF:000001">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.10:FF:000003">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.11.10:FF:000001">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.15.10:FF:000002">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.11.10">
        <property type="entry name" value="DNA Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.15.10">
        <property type="entry name" value="Topoisomerase I, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.41">
        <property type="entry name" value="Yeast DNA topoisomerase - domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013034">
        <property type="entry name" value="DNA_topo_DNA_db_N_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013030">
        <property type="entry name" value="DNA_topo_DNA_db_N_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001631">
        <property type="entry name" value="TopoI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025834">
        <property type="entry name" value="TopoI_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014711">
        <property type="entry name" value="TopoI_cat_a-hlx-sub_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014727">
        <property type="entry name" value="TopoI_cat_a/b-sub_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013500">
        <property type="entry name" value="TopoI_cat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008336">
        <property type="entry name" value="TopoI_DNA-bd_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036202">
        <property type="entry name" value="TopoI_DNA-bd_euk_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013499">
        <property type="entry name" value="TopoI_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018521">
        <property type="entry name" value="TopoIB_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048045">
        <property type="entry name" value="Topoisomer_I_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051062">
        <property type="entry name" value="Topoisomerase_IB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290:SF3">
        <property type="entry name" value="DNA TOPOISOMERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290">
        <property type="entry name" value="DNA TOPOISOMERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14370">
        <property type="entry name" value="Topo_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01028">
        <property type="entry name" value="Topoisom_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02919">
        <property type="entry name" value="Topoisom_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00416">
        <property type="entry name" value="EUTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00435">
        <property type="entry name" value="TOPEUc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56741">
        <property type="entry name" value="Eukaryotic DNA topoisomerase I, N-terminal DNA-binding fragment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00176">
        <property type="entry name" value="TOPO_IB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52038">
        <property type="entry name" value="TOPO_IB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="DNA topoisomerase I eukaryotic-type" evidence="10">
        <location>
            <begin position="305"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="639"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="83"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="96"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="6">
        <location>
            <position position="739"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="781" mass="90689" checksum="6AE1AED7895AE23E" modified="2011-11-16" version="1">MSSSEEDDIVLSRIPKKRHIISEMTPPTSIDEDDIPLKNSGKRKNTIAEDDDYEEDVPLVKKAKKANGSALKKVKKEPKTPKKTPTTPKATTTKAKAADKKTTPKATPKKETKSVSATPKSRKVAIKEESVKQETPMSSQEDEEKYKWWEAQDLDGEQKWESLEHNGVMFPPEYEPLPSHVKLYYDGKPVNLPLQAEEVAGFYAAMLETDHAKNPVFQKNFFDDFCQVLKECGGCGVDINDFTKMDFSKMYAHFEKLREEKKLMSREEKKQIKLEKERLEEPYKTCYLNGRKELVGNFRIEPPGLFRGRGAHPKTGKLKRRVKPEDVTLNLGRDVKVPEPPAGHKWGEIRHDNTVTWLAMWKENIADSFKYVRFAASSSVKGQSDFKKFETARNLRNHIDAIRKDYTEMLRSEKMQDRQMATATYLIDVFALRAGGEKGEDEADTVGCCSLRFEHITLKPPNKVIFDFLGKDSIRFYQEVEVDKQVFKNLRIFKKEPKQPGDDLFDRINPSILNKHFQNYMKGLTAKVFRTYNASKTMQDQLDLIPNEGTIAEKVVKFNAANRTVAILCNHQRSVSKNHGTSVQKISDKLKEYMWQKIRLKRMILVLEPKLRKKDPAYFEEIDDLVKEDEEHIHHTIINRQREQVMKKLQRDNEKLKLEKKPLLTEKDVKDKLDKIDELEKEYRQELKKGKPEVKSNMTVEKLKQQVETIEARYVTTSLQLKDKEDNSEVSLGTSKMNYIDPRLTVMFSKKYGVPIEKLFTKTLRDKFKWAIESADENWRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATX7</accession>
    <name>G3ATX7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_63208</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the gemin-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377324.1">
        <property type="nucleotide sequence ID" value="XM_007377262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATX7"/>
    <dbReference type="STRING" id="619300.G3ATX7"/>
    <dbReference type="GeneID" id="18874641"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63208"/>
    <dbReference type="eggNOG" id="ENOG502SCAA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083380_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATX7"/>
    <dbReference type="OMA" id="SQWIWKL"/>
    <dbReference type="OrthoDB" id="428895at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032797">
        <property type="term" value="C:SMN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035426">
        <property type="entry name" value="Gemin2/Brr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12794:SF0">
        <property type="entry name" value="GEM-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12794">
        <property type="entry name" value="GEMIN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04938">
        <property type="entry name" value="SIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="48"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="235" mass="27191" checksum="4C306802E69EB538" modified="2011-11-16" version="1">MDAVHEYGPLQVLPIEDSNEDGESSDVYKYLLSVRDEAWNGSPVEFIDREIKSEPEPTQEEQEQEQEKEQEQSAEVTEEAQEGVTLWQQQLLAEFKDIKQQLAQVDQPATLLEIPEDASHWRKFILNNEPPTIEYFVHQLDKPTKVKLLVYITKWLTMSNASIANLSQWIWKILLTIDTRSMLTPDEIFVLRKLAKKTIKVKETSTETSSVGNMTIDMIITLVCRHYGQLDLLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU59</accession>
    <name>G3AU59_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lariat debranching enzyme C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_143265</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377406.1">
        <property type="nucleotide sequence ID" value="XM_007377344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU59"/>
    <dbReference type="FunCoup" id="G3AU59">
        <property type="interactions" value="679"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU59"/>
    <dbReference type="GeneID" id="18870530"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143265"/>
    <dbReference type="eggNOG" id="KOG2863">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005893_1_0_1"/>
    <dbReference type="InParanoid" id="G3AU59"/>
    <dbReference type="OMA" id="KWWFSAH"/>
    <dbReference type="OrthoDB" id="407609at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008419">
        <property type="term" value="F:RNA lariat debranching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032197">
        <property type="term" value="P:retrotransposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016074">
        <property type="term" value="P:sno(s)RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007708">
        <property type="entry name" value="DBR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12849:SF0">
        <property type="entry name" value="LARIAT DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12849">
        <property type="entry name" value="RNA LARIAT DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05011">
        <property type="entry name" value="DBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01124">
        <property type="entry name" value="DBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lariat debranching enzyme C-terminal" evidence="1">
        <location>
            <begin position="290"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="444" mass="51324" checksum="7965CC7E956F285F" modified="2011-11-16" version="1">MSSVRIAIQGCAHGELEQIYAKIDPKTDLLLILGDFQALRSTQDYQALNVPAKYRALGDFHSYYSGALTAPCLTIFIGGNHENSAYLQELKYGGWVAPRMYYLGEFGSVWYRGIQIAGWSGIFNRSTFLRNNMYVEKPPYRRDELVSVYHQKLTAFIKLYMMNHDLDVVMSHDWPVGIEDYGDKRKLLALKPFFKKDIENKELGSPLNKFLLHHLRPRYWFSAHLHVLFEASVSYKGEVNSKEQSKSSNNDEIALNMDDETEKEISNKDKINLDMDEEDLYLSNPQPKKTPPRMSPSDHSTNFLALDKCGKRRIHLRHITITPDEAHQSHPSYKDNKLYYSRRAIAINKVLETYLKSHEDEFRGINTRKILEDPHNLVLVNELMPLVELELRKLSGMKDELFIVPESFKVVAPTEDDGSQLKFYPNNQTEEYCKKFDVVSPDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUB2</accession>
    <name>G3AUB2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Nucleoporin GLE2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_63309</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat rae1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377459.1">
        <property type="nucleotide sequence ID" value="XM_007377397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUB2"/>
    <dbReference type="FunCoup" id="G3AUB2">
        <property type="interactions" value="1294"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUB2"/>
    <dbReference type="GeneID" id="18874700"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63309"/>
    <dbReference type="eggNOG" id="KOG0647">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038526_1_0_1"/>
    <dbReference type="InParanoid" id="G3AUB2"/>
    <dbReference type="OMA" id="EAMDQSI"/>
    <dbReference type="OrthoDB" id="256303at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051664">
        <property type="term" value="P:nuclear pore localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000728">
        <property type="term" value="P:regulation of mRNA export from nucleus in response to heat stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032185">
        <property type="term" value="P:septin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000190">
        <property type="entry name" value="Nuclear pore complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10971">
        <property type="entry name" value="MRNA EXPORT FACTOR AND BUB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="30"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="5">
        <location>
            <begin position="41"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="75"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="116"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="271"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="371" mass="40945" checksum="DD376EC6399D5151" modified="2011-11-16" version="1">MSFLSTGTTSAATNSATANGQELINDITINNPPTDTITDIAFSPQQELLAVSSWDKKVRIYEIDSNSGNNQGRAMFEHDAPVFSARWSIDGTKVVSGGGDGQVKIYDIASGSTQQIGQHDNVVRAVRYVECGATNTPVVVSGSWDKTLRYWDMRSPQPITTIQLPERVYCMDSSQKLLVVGGADRHISVIDLNNPQQIFKSYQSPLKWQTRTVCCYPQANGFAIGSIEGRCAIQYINDNEQKKFGFSFKCHRKSGSSSTTSTTTRTTTSSSESQAYSVNAISFHPVYGTFSTAGSDGTFCFWDKDAKQRLKSFPVLPGTITATAFNKTGNIFAYAVSYDWSMGYMANRQDYPTMIKLHATKDDEIKQKNKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUC4</accession>
    <name>G3AUC4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 51 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63319</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377471.1">
        <property type="nucleotide sequence ID" value="XM_007377409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUC4"/>
    <dbReference type="STRING" id="619300.G3AUC4"/>
    <dbReference type="GeneID" id="18874707"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63319"/>
    <dbReference type="eggNOG" id="ENOG502SDB2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062460_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUC4"/>
    <dbReference type="OMA" id="LNTHDSE"/>
    <dbReference type="OrthoDB" id="124041at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="245" mass="27813" checksum="12BE5F0AD82EDB3F" modified="2011-11-16" version="1">MSEPILSYKRPSSRESPASPAVPASSPNPQSQSASPKVKHHAGARKVSSRRRALQDFYNISSDEPTTKKSDPDSLPSPEELNNIIQNSSINDILKLRNRITHTLNSQDSAKKSIIYDNYYELIKLSETLSDLSKGMHNEVNGLGIYEEKQESKEKFMDDVLSDLHDFINSEDTKMFNRPFEEILDVPQEVTSSKVLDEVNQLLNIKGNLDEQEKEKVASQIDSLIEKLPQHELLQLQLNKLKQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUR9</accession>
    <name>G3AUR9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peptidase A22B, signal peptide peptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_63453</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase A22B family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377596.1">
        <property type="nucleotide sequence ID" value="XM_007377534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUR9"/>
    <dbReference type="STRING" id="619300.G3AUR9"/>
    <dbReference type="GeneID" id="18874760"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63453"/>
    <dbReference type="eggNOG" id="KOG2443">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1071835_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUR9"/>
    <dbReference type="OrthoDB" id="29661at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098553">
        <property type="term" value="C:lumenal side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033619">
        <property type="term" value="P:membrane protein proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007369">
        <property type="entry name" value="Peptidase_A22B_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006639">
        <property type="entry name" value="Preselin/SPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174:SF23">
        <property type="entry name" value="MINOR HISTOCOMPATIBILITY ANTIGEN H13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04258">
        <property type="entry name" value="Peptidase_A22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00730">
        <property type="entry name" value="PSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="25"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="229"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="247"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="261" mass="29665" checksum="CEA0AC4C5FEB5884" modified="2011-11-16" version="1" fragment="single">MKFVASFVVTSVVMGLHYHFPNNWIIGNILGVSFVISTLSQIKLNQFKLVYLLLSGLFFYDIYFVFGTDIMETVATGLEVPMKLLMPRIGSQFSLLGLGDVVVPGFLISLCLRFDIYQYYARNDVSFHHLNNYAQPYFKASLVSYVLGLLLTFSMLHIFQVGQPALLYIVPCLLIGVTGLSLFRQEFTEFWSFSEDISEFVDEKDKEEGEDYQVKEEGDNVEANLVDDVYEDGDGSNDGEYSNNDSEKDDDEEEDATYFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUU3</accession>
    <name>G3AUU3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_158385</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377800.1">
        <property type="nucleotide sequence ID" value="XM_007377738.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUU3"/>
    <dbReference type="GeneID" id="18871123"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_158385"/>
    <dbReference type="eggNOG" id="KOG1339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013253_9_1_1"/>
    <dbReference type="InParanoid" id="G3AUU3"/>
    <dbReference type="OMA" id="VANCKAR"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A1" evidence="4">
        <location>
            <begin position="1"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="296"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="381" mass="41363" checksum="BCA73C6EE0C0E4A2" modified="2011-11-16" version="1">MDSNVECKTSPLTSKVANCKARGTFSSEKSETFNTTKQEFCMAYMDNSYGKGIYGKDDISLGKDVNIKGFEFGLAREATSDVGILGISFPGGNPAGRCFSEELKNQGVINRNAYSIFLNEANKTGSVLFGAVDHSKYLGDLKTVPIVYDETNPEIKVNVLGVDIIMNDAKVSGGISESSFLLDTGTAVSQFPKSLLAPLLGQIKDSKYDASNKTISFPDCSQIENLKFEIKFDGKTIIAPANNFMVERDADGCTFEVTFIDSDFILGIDALRSIYFVVDLDDKEVSLAQSDFSNGKQDIEVFPPPGVDRTSASTGISDHQTHLSRPQHHASTSIHRHQEPSATSEHHEPSATSEHKPSSASSIVSSIDVLLCLVISLTWLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUV0</accession>
    <name>G3AUV0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LSM complex subunit LSM4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LSM4</name>
        <name evidence="12" type="ORF">SPAPADRAFT_57515</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">LSm subunits form a heteromer with a doughnut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377807.1">
        <property type="nucleotide sequence ID" value="XM_007377745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUV0"/>
    <dbReference type="STRING" id="619300.G3AUV0"/>
    <dbReference type="GeneID" id="18872124"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57515"/>
    <dbReference type="eggNOG" id="KOG3293">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099537_0_1_1"/>
    <dbReference type="InParanoid" id="G3AUV0"/>
    <dbReference type="OMA" id="VACDAWM"/>
    <dbReference type="OrthoDB" id="747253at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005732">
        <property type="term" value="C:sno(s)RNA-containing ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01723">
        <property type="entry name" value="LSm4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034101">
        <property type="entry name" value="Lsm4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="2"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="88"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW30041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="140" mass="16281" checksum="13832467C0143ADC" modified="2011-11-16" version="1">MLPLYLLTAAKNKPVLIELKTGETYQGDLVNLDPWMNLTLSNVIHTNTGADEFSKETEVYIRGMHIKYLRIPDEIMDYAKEQNLLNMEQRNKNVKRRGGDRNQSGGGGRDHRRGGRGGNRRFQGDRHYNNNHHYQQRDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUX0</accession>
    <name>G3AUX0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial acidic protein MAM33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_57528</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377827.1">
        <property type="nucleotide sequence ID" value="XM_007377765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUX0"/>
    <dbReference type="FunCoup" id="G3AUX0">
        <property type="interactions" value="533"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUX0"/>
    <dbReference type="GeneID" id="18872125"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57528"/>
    <dbReference type="eggNOG" id="KOG2536">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072692_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUX0"/>
    <dbReference type="OMA" id="CEYMMSK"/>
    <dbReference type="OrthoDB" id="278212at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.280.10">
        <property type="entry name" value="Mitochondrial glycoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003428">
        <property type="entry name" value="MAM33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036561">
        <property type="entry name" value="MAM33_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826">
        <property type="entry name" value="COMPLEMENT COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826:SF1">
        <property type="entry name" value="COMPLEMENT COMPONENT 1 Q SUBCOMPONENT-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02330">
        <property type="entry name" value="MAM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54529">
        <property type="entry name" value="Mitochondrial glycoprotein MAM33-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="270" mass="30517" checksum="427066AFFA4B7AC2" modified="2011-11-16" version="1">MSKLLSSTLRTQLSKRLAIATSRSIRTPVIAALPKLVASTSIRSFSSTQIIRKQSSSESSLKAVIEGELKVAEEIPNELDAGFEEYLSSQGYKVITKEGNANVELIRTDASGHVIHVYFDVEEVTDLSEEFSEFQEENEWADEDEDSESFGNGFFSKFKVFIEDPATNDGLNFDLFLKNIESGFSVECVNYQPNATEFISEVSKGNFTDKFRYEGPKFEDLDESLQVEFENYLTAKGIDEKLAEFIVAYSEHKEESEYRGWLSSISKFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV49</accession>
    <name>G3AV49_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_68946</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377889.1">
        <property type="nucleotide sequence ID" value="XM_007377827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV49"/>
    <dbReference type="GeneID" id="18875248"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68946"/>
    <dbReference type="HOGENOM" id="CLU_667589_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV49"/>
    <dbReference type="OrthoDB" id="4028033at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22143">
        <property type="entry name" value="F-box_ScMDM30-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="13"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="412" mass="47837" checksum="F8785A81695EBD6E" modified="2011-11-16" version="1">MRGAKGDLVSTESFALLSLPDEVTTQIIAVLSQIDILSLIQVNKHLYSLGLPKLYHSIYLNNSTSCTPLAISSEINYPFHIKYTIINWITFFIRQMENNPNKNLVKEIVTNCIEPDDFKTIQEMFPKVNITIEYDSELYRFRSLDLSMVNGLELTYENALFFDENIRGKNYYSIKEVGINEESDHDLFGIIPVLKGLESMHITSVSDRTFTSLKDAGVSKLAIKRLFLQNFITPNNPGDLFRDLDEYFNLESLETFGFDSKYDSLFQDLQLTIPRLSNLKHLFLHGNRKCCFDASMILPRDSLEFLCLHDSKENGEFFDYDSLMTIISRQESTLTSLCLQCNIYHYMGYEWVDRVYNYLHEVTEIDNRRNIHLINQILAKGTRFPKLDTIIINEKYYFIKRNGPGDIKINTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVD9</accession>
    <name>G3AVD9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Copper-fist domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_63766</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377924.1">
        <property type="nucleotide sequence ID" value="XM_007377862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVD9"/>
    <dbReference type="FunCoup" id="G3AVD9">
        <property type="interactions" value="500"/>
    </dbReference>
    <dbReference type="GeneID" id="18874904"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63766"/>
    <dbReference type="eggNOG" id="ENOG502QQ0T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031396_1_0_1"/>
    <dbReference type="InParanoid" id="G3AVD9"/>
    <dbReference type="OMA" id="CTNCETH"/>
    <dbReference type="OrthoDB" id="5600085at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.430.10">
        <property type="entry name" value="Copper fist DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051763">
        <property type="entry name" value="Copper_Homeo_Regul"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001083">
        <property type="entry name" value="Cu_fist_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036395">
        <property type="entry name" value="Cu_fist_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28088">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28088:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00649">
        <property type="entry name" value="Copper-fist"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00617">
        <property type="entry name" value="COPPERFIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM01090">
        <property type="entry name" value="Copper-fist"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00412">
        <property type="entry name" value="Cu_FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57879">
        <property type="entry name" value="Zinc domain conserved in yeast copper-regulated transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50073">
        <property type="entry name" value="COPPER_FIST_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Copper-fist" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="298"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="412"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="326"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="429"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="462" mass="50578" checksum="FB8606198EE15528" modified="2011-11-16" version="1">MECIRGHRSSSCKHHTRPLLQVRSKGRPNVYANGNPNHRIAVFAEEIAKEEPDSPDFELNKCKKSQPVVILKASPKQVIDLVSGVIVGPYDEASCQPSNVKPKTTPPVINSDSFINSSTCCNPTVHSSNVSKPNKSCGCCANKKKPVNKSKILESYIKKKIEKRINVKSQPKSTGWTWTFVNEYSPEADPSITVAPTKIKTEDESQIEQPQTAPSSSSSTQIFDVVSIPSCSIPGTCSCDENCTCAGCMVHGNSKASMNEIAKYLPLPDSNFNNNLQDSGNIIFNSVPGLPGHITTYPHNQRHDNSYANQFPNPTLTQSSPYQQDTVSNSSATASTGSASPNVCSCPSDGCDCTNCETHGIINGYKLDDYFKTHNFTSMNDLFNDFPMNGGEDIYSVMKQENDLLYEQLLKPSAGDPERSLLQQSQQQPPQPQLPPQPPQPPQPQQPPQHPQPPSRSCCSKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVE8</accession>
    <name>G3AVE8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_144298</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1-chloro-2,4-dinitrobenzene + glutathione = 2,4-dinitrophenyl-S-glutathione + chloride + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51220"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:34718"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:133977"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 glutathione + H2O2 = glutathione disulfide + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:16833"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="EC" id="1.11.1.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377933.1">
        <property type="nucleotide sequence ID" value="XM_007377871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVE8"/>
    <dbReference type="STRING" id="619300.G3AVE8"/>
    <dbReference type="GeneID" id="18870613"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144298"/>
    <dbReference type="eggNOG" id="KOG1752">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026126_7_2_1"/>
    <dbReference type="InParanoid" id="G3AVE8"/>
    <dbReference type="OMA" id="YACYELD"/>
    <dbReference type="OrthoDB" id="418495at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015038">
        <property type="term" value="F:glutathione disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000026">
        <property type="entry name" value="Glutaredoxin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011767">
        <property type="entry name" value="GLR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011899">
        <property type="entry name" value="Glutaredoxin_euk/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02180">
        <property type="entry name" value="GRX_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694:SF26">
        <property type="entry name" value="GRX1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00195">
        <property type="entry name" value="GLUTAREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="8">
        <location>
            <begin position="31"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW30167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="113" mass="12549" checksum="D174ED038D9E3974" modified="2011-11-16" version="1">MFEFITSWFKSEPISPKIIEDINSTINSHKVVVYSKTYCPYCKSTKELFGKLNQDFKVVELDNVSDGSVIQRGLKEITGQGTVPNIFINGKQIGGNSDLQSLYSQGKLLGLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVI5</accession>
    <name>G3AVI5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63557</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377700.1">
        <property type="nucleotide sequence ID" value="XM_007377638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVI5"/>
    <dbReference type="FunCoup" id="G3AVI5">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVI5"/>
    <dbReference type="GeneID" id="18874804"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63557"/>
    <dbReference type="eggNOG" id="ENOG502SA1D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100671_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVI5"/>
    <dbReference type="OMA" id="QEWEDQT"/>
    <dbReference type="OrthoDB" id="2135762at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000444">
        <property type="term" value="C:MIS12/MIND type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013950">
        <property type="entry name" value="Mis14/Nsl1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31749">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN NSL1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31749:SF3">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN NSL1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08641">
        <property type="entry name" value="Mis14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="173"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW29934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="223" mass="26190" checksum="384050F2AF5FCE32" modified="2011-11-16" version="1">MDQSPDDGKITLSRDDLRYVYNQLYISTMDRLNLHLPTEENDPMKNQVATILNEFLLNVFDDARHSLIVDGVADDKPISEVLALPERQEVVEPFDVELNQQLKDTLLEFENEIVKSTTQKRELPKQAAAEYEKFVDRVDQGVSEYLAKIDEEVEVINNQESFDFEIEDKDIPYEELRAKYLDTLEKLDELKQIVPETESRLERDLQTMAFLEQAYERQKREEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVN6</accession>
    <name>G3AVN6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cysteine desulfurase, mitochondrial</fullName>
            <ecNumber evidence="3">2.8.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">tRNA-splicing protein SPL1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_57634</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. Plays a role in both tRNA-processing and mitochondrial metabolism. Involved in the 2-thio-modification of both 5-carboxymethylaminomethyl-2-thiouridine in mitochondrial tRNAs and 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U) in cytoplasmic tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(sulfur carrier)-H + L-cysteine = (sulfur carrier)-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="3"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377967.1">
        <property type="nucleotide sequence ID" value="XM_007377905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVN6"/>
    <dbReference type="FunCoup" id="G3AVN6">
        <property type="interactions" value="899"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVN6"/>
    <dbReference type="GeneID" id="18872140"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57634"/>
    <dbReference type="eggNOG" id="KOG1549">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_0_2_1"/>
    <dbReference type="InParanoid" id="G3AVN6"/>
    <dbReference type="OMA" id="KGLYWAR"/>
    <dbReference type="OrthoDB" id="10250117at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990221">
        <property type="term" value="C:L-cysteine desulfurase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070903">
        <property type="term" value="P:mitochondrial tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000003">
        <property type="entry name" value="Cysteine desulfurase IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000002">
        <property type="entry name" value="Cysteine desulfurase IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00331">
        <property type="entry name" value="Cys_desulf_IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010240">
        <property type="entry name" value="Cys_deSase_IscS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02006">
        <property type="entry name" value="IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002806">
        <property type="entry name" value="PRK02948.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010611">
        <property type="entry name" value="PRK14012.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601:SF34">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601">
        <property type="entry name" value="CYSTEINE DESULFURYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="16">
        <location>
            <begin position="76"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00080923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW30201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="475" mass="52698" checksum="DC689E3C7B625AD7" modified="2011-11-16" version="1">MYRRLVAFSRKPLRKSTVGASSVRPFSQTIIETSNPVTGELHITTNFTNGTQPPKGSTISLKSAKRDATQFGTRPIYLDVQATTPTDPRVLDKMMEFYTGLYGNPHSSTHSYGWETDREVEKAREYIADVINADPKEIIFTSGATESNNMVIKGVPRFYKNTKKHIITTQTEHKCVLDSARHMQDEGFEVTYLPVDSHGLINLDDLKNAIRKDTALVSIMAVNNEIGVIQPIKEIGEICRKNKIFFHTDAAQAFGKIPIDVNEMKIDLLSISSHKIYGPKGIGAVYVRRRPRVRLEPLINGGGQERGLRSGTLAPPLVAGFGEAARLIKQEMEYDQKYIDRLSNKLKSGLLAIPSTQLNGSEDPNSQYPGCVNVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVLHALGADDALAHSSIRFGIGRFTTEQEVDYVIQAINERVEFLRKMSPLWEMVQEGIDLNSIEWSGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVQ7</accession>
    <name>G3AVQ7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_63841</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377988.1">
        <property type="nucleotide sequence ID" value="XM_007377926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVQ7"/>
    <dbReference type="FunCoup" id="G3AVQ7">
        <property type="interactions" value="335"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVQ7"/>
    <dbReference type="GeneID" id="18874938"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63841"/>
    <dbReference type="eggNOG" id="KOG1303">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009646_8_2_1"/>
    <dbReference type="InParanoid" id="G3AVQ7"/>
    <dbReference type="OMA" id="MKWTHIA"/>
    <dbReference type="OrthoDB" id="655540at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015186">
        <property type="term" value="F:L-glutamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005290">
        <property type="term" value="F:L-histidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015188">
        <property type="term" value="F:L-isoleucine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090513">
        <property type="term" value="P:L-histidine transmembrane import into vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015824">
        <property type="term" value="P:proline transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF692">
        <property type="entry name" value="TRANSMEMBRANE AMINO ACID TRANSPORTER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="335"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="417"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="455"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="510"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="533"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="568"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="9">
        <location>
            <begin position="195"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="591" mass="64575" checksum="46A54B04D6F010D9" modified="2011-11-16" version="1">MTSYGQHPDENSHLLASRRPSLAFLDTPIGSFKGPNSLHNFASSFTRAQSFAATKIDNDIHKKRSFFTGGEDDGEAIDDETFDPELMIPSIRGERLSTVIHDLQNKNQLFMNDEVDNEVFYHDDIMSALYETRSRQSADHATVPPSAIPTLRKRPYGASFSSIRSAISTATSASHVTLKKIEDSEGNVVTVLAGQSTAPQTIFNSVNVLIGVGLLALPVGIMKAGWILGIPILVLCGLTTYWTACLLSKAMDTDYTIMTYADLGYAAYGSTAKLIISLLFSVDLLGAGVSLIVLFSDSLYALLGDDQIWTRTTFKLISFLVLTPFTFVPLPILSIISLFGILSTISITILVMICGFLKPTSPGSLLQIMPTNLWPQSIPDFLLAIGILMAPFGGHAIFPNLKSDMRHPYKFNKTLRYTYFITLITDCSMGVLGFLMFGNKCSNEITNTLLLTPGYPVWCYPLISGLICIIPLAKTPLNAKPIISTLDVLLGVNNITTSSFRKGLNSIGKFFIRVGVNAVFVILAILFPEFDKIIGMLGASICFIICIILPCTFYLKLCGGNIKGFEKFIVWAAIIISTILAFAATYAVILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVR1</accession>
    <name>G3AVR1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_63847</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Fluoride channel required for the rapid expulsion of cytoplasmic fluoride.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>fluoride(in) = fluoride(out)</text>
            <dbReference type="Rhea" id="RHEA:76159"/>
            <dbReference type="ChEBI" id="CHEBI:17051"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:76160"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the fluoride channel Fluc/FEX (TC 1.A.43) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377992.1">
        <property type="nucleotide sequence ID" value="XM_007377930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVR1"/>
    <dbReference type="FunCoup" id="G3AVR1">
        <property type="interactions" value="128"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVR1"/>
    <dbReference type="GeneID" id="18874940"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63847"/>
    <dbReference type="eggNOG" id="ENOG502RZ3R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030507_1_2_1"/>
    <dbReference type="InParanoid" id="G3AVR1"/>
    <dbReference type="OMA" id="CYDLQHV"/>
    <dbReference type="OrthoDB" id="409792at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903425">
        <property type="term" value="F:fluoride transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003691">
        <property type="entry name" value="FluC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259">
        <property type="entry name" value="FLUORIDE EXPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259:SF1">
        <property type="entry name" value="FLUORIDE EXPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02537">
        <property type="entry name" value="CRCB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="376"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="409" mass="44866" checksum="8A9B83E7FAD189C0" modified="2011-11-16" version="1">MSFSSSRDSMSTGADVERRASLSGGSMIRRRSSIESTRLQRRRTIEEAFTEVAAENVAHALPEGFNAKFADFRETTFLVKTKKYHTIIWNIIHGAIWGVLVRRGLTALTSYNGSYLSGVVWANFTACVVMGIAVDGEELWSILVDDHADIYPHKGAIPLYTGITTGFCGTVSSFSSVILEAFNKAADTEIGIHYNYPNGAYGIMQFLAVILAQFGLSIMGFHIGKQTSAVIDNYVRPITKRQYKIIEYISVTTGVCLVIITCVLIGVKHDGAWRSWTFSMFFAPFGAVLRFYLSKYLNSKVENFPMGTFTANVGGSLLLGIFTLIGRGKFGDNGRLNNSIIGCHVLNGLDDGFCGGLTTVSTFVVELFGLKTVFGYQYGVSSIMLSFIMVILVLGSYNWTVGLTDPYCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVV6</accession>
    <name>G3AVV6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63628</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377767.1">
        <property type="nucleotide sequence ID" value="XM_007377705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVV6"/>
    <dbReference type="GeneID" id="18874838"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63628"/>
    <dbReference type="HOGENOM" id="CLU_1932663_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVV6"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="131" mass="14471" checksum="DDE1C653BCC884DD" modified="2011-11-16" version="1" fragment="single">MCSQSILDENQEGLGPELQKLVIEDKDENADFEVQENLNENIHTASGIENEKSFQESFSNCTDIGDVTDQESTRDTSEIANVSQSSSDANYVQENVPDSSVRKDTTTYAKEIPGIPVTFQVKISDQKSASF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AED4</accession>
    <name>G3AED4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_131008</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373134.1">
        <property type="nucleotide sequence ID" value="XM_007373072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AED4"/>
    <dbReference type="FunCoup" id="G3AED4">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AED4"/>
    <dbReference type="GeneID" id="18869546"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131008"/>
    <dbReference type="eggNOG" id="KOG4347">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003538_0_1_1"/>
    <dbReference type="InParanoid" id="G3AED4"/>
    <dbReference type="OMA" id="IYMSETQ"/>
    <dbReference type="OrthoDB" id="17687at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000021">
        <property type="entry name" value="GTPase activating protein (Gyp2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000015">
        <property type="entry name" value="GTPase activating protein (Gyp2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF20">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="238"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="589"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="839"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="864"/>
            <end position="874"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW35722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="926" mass="106056" checksum="9719DE1465EAB517" modified="2011-11-16" version="1">MAFFDALREKAVTTLNQVLDKNRNSGLTKHQQFCYSHHIPETETILSESAAEIGIKSDYSTDAYNDNLPFPQGRIYLTQSFLIFRDAFDRKNCSFSVHLSTIKKVERPPGSDYGFALMVSTYSKLHLTLYLVGIRSDSERFAQQLKIALKKNLVHVGKLQPFIQTCYSEYLLAKNKVSTEKVEHAPQGGLGLVFKFPGDPKESRDKTKMKLWFDLFRVDGRNLSLVRTPLFYKLVRVGLPNRLRGEIWELCCGSMYLRLDYQGEYNKILEDHKDQKSFAIEEIEKDLNRSLPEYAAYQSSQGIERLRRVLTAYSWKNPDVGYCQAMNIVVAAMLIYMSEEQAFWCLNVLCDRIVPGYYSKTMYGTLLDQKVFESLVQKTMPMLWEHITKNDIQLSVVSLPWFLSLYLSSMPLVFAFRILDVFFLQGPKTLFQVALAVLKLNGEALLNTEDDGTFISIIKQYFLTLHESAHPNSPNPKFRSITRFQELLVTAFREFSVIDEEMINNTRAKHRDTIYQNISSFVKRTELRNLPRTTNISQETLSLLYDRFYSVVESLSVTKGSGTSVMDYKVFVEFMSEICDWITDVNVDDERHFLRRLFNHWDTEKQGALTFTDLLVGLNSLVDPDLMTSMSNFFELYDTKGDGKIDREGILQISEDLLYITSPWKDGVLFDGITKANIENVIADEVYKKQVESGNTSPDQSEIQLPSDFQVDRDKLEQQQVERYLQAAATFIQRAFEYAQPEEEELLIKELAIDNSISHNAALNPNTPVYLNLPTFRMVVLADETYELLFGSTLREATHLERPLDTKFDPIRNLRDMFDGLLADGRKVATKVRLRMDSRAANGGGGAPPNSSNASIKSGAKREEEEEDQDDDDLGVISIDDNDKDLLLATEAQVVVKESTEKPRISRMESEKIIGKSDQNLIEFET</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3AET7</accession>
    <name>G3AET7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58966</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373179.1">
        <property type="nucleotide sequence ID" value="XM_007373117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AET7"/>
    <dbReference type="STRING" id="619300.G3AET7"/>
    <dbReference type="GeneID" id="18872701"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58966"/>
    <dbReference type="eggNOG" id="KOG1940">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049048_0_0_1"/>
    <dbReference type="InParanoid" id="G3AET7"/>
    <dbReference type="OMA" id="PRGFAYN"/>
    <dbReference type="OrthoDB" id="10253329at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="424" mass="48903" checksum="147E4940C40922D8" modified="2011-11-16" version="1">MDNQANQSAQASQVPAQEKKRPPRRRPKKKSDEQKHDLTSTKKFLQISKLIRAFKPVTINGSPTQTIIDQIKLKESNDPDYFKTKYKNHARLSNLQKYLAEFIESQPDSTIYISMCVKPSDPDFPFDLDKLQLNLSIPGKYPDKVQKPKIVVLNDEIPRGFAINVENGFKRIAEIALNKGEDEEIELIQGTGLLSMILTLDKYLEEFLKQEKRETFKFVKTVKKSSPKPKDASPVPAPAPTPSPKEDKKHSPAVKRQEQIIVSPEVARLRDSLTEELINKLGDYIKVFKKSHQGETLYKIALPIFNLDSPVIPNLWKLNEQVELMVAIPINFPQSKLKLTFPNNFNSNLILKYCQQDEQQFELVQITKQYRKIEQNFTNNFNKFDFGTNSLVSIFNWLTNNIGQFSLSDQEFKQWVSNIEKFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEU4</accession>
    <name>G3AEU4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_64855</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373186.1">
        <property type="nucleotide sequence ID" value="XM_007373124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEU4"/>
    <dbReference type="FunCoup" id="G3AEU4">
        <property type="interactions" value="140"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEU4"/>
    <dbReference type="GeneID" id="18875002"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64855"/>
    <dbReference type="eggNOG" id="KOG1363">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_413380_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEU4"/>
    <dbReference type="OMA" id="FYMFIEL"/>
    <dbReference type="OrthoDB" id="1026733at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF1">
        <property type="entry name" value="FAS-ASSOCIATED FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="486"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="627" mass="72692" checksum="EFCF74CA3211B912" modified="2011-11-16" version="1">MSDDLTADQQEAVVQFKSITGIENDENDDKVLRLLVIHEYNLNNAISTYFDSGFEMAETANTYSSGAQVHHDGQLHNRERTASPPRHSPTPPARDNIMNLQQQMFMDNLMPRFPKAPTISNGWQLEVGIHTSLIHEREEQQKKLLENEEDSESTISVPPTVVDEGKERRSPAAALWVLLLILPKTIWNILVSAIRFLFGLDTTSGSGESSKLSRTFNFDKFHPSYRLLDQVNSWIKKTELIKDDETLAEKESFEPEDSSTLVESNFNISESDFNNAHQISQREYHWLLVILVNDSVGCKRFVRELFANSGFNELFNKNSGTFKETQIFISNVERNREAFEVAHVYKVRRTPYIMLVGNVSPSPDIMASMSILYKSNLSTPFVSNDYEARLTVIKIIKNVNKFLEKFTPQLISARYDRQEMEFSRMIRQQQDDAYLQSLEQDRQKKVQREKEEKAKHDLEISYKMRQYYLLNLVKYDFFSSVTTTGEDEDKFRIAVKLPSGKRLVEFFRKSITIKEFYMFIELKLFVEELLETQGAEFEEITHIIDELIADVGLPRSLQEQPLTLEDYCVKYPFKFEVIQPYPKKVIVANDVKISEAPDFKGANFLVEFTCEDEDEDEDEEVDEESEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEY4</accession>
    <name>G3AEY4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_57859</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372200.1">
        <property type="nucleotide sequence ID" value="XM_007372138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEY4"/>
    <dbReference type="FunCoup" id="G3AEY4">
        <property type="interactions" value="1437"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEY4"/>
    <dbReference type="GeneID" id="18872223"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57859"/>
    <dbReference type="eggNOG" id="KOG1375">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015718_1_1_1"/>
    <dbReference type="InParanoid" id="G3AEY4"/>
    <dbReference type="OMA" id="WVPRSVN"/>
    <dbReference type="OrthoDB" id="1662883at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045298">
        <property type="term" value="C:tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000022">
        <property type="term" value="P:mitotic spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903087">
        <property type="term" value="P:mitotic spindle pole body duplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098863">
        <property type="term" value="P:nuclear migration by microtubule mediated pushing forces"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090316">
        <property type="term" value="P:positive regulation of intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000003">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000009">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="14">
        <location>
            <begin position="47"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="15">
        <location>
            <begin position="246"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW34788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="448" mass="49783" checksum="B3577E5BF8B39A78" modified="2011-11-16" version="1">MREIIHLSTGQCGNQIGAAFWETICGEHGLDNNGTYSGEDDIQKAKLDVYFNEATSGKYVPRAVLVDLEPGTIDNVKTSKIGGLFRPDNYIFGQSSAGNVWAKGHYTEGAELVDSVLDVVRREAEGCDSLQGFQITHSLGGGTGSGMGTLLISKIREEFPDRMMATFSVVPSPKVSDTVIEPYNATLSIHQLVDNSDETFCIDNEALYNICQKTLKLPQPSYAELNSLVSSVMSGVTTSLRYPGQLNSDLRKLAVNLVPFPRLHFFMVGYAPLTSIGAKSFRSVTVPELTQQMFDSKNMMAASDPRNGRYLTVAAFFRGKVSVKEVDDEMHKIQTKNAAHFVEWIPNNVQTAVCSVPPRDLDMSATFIGNSTSIQELFKRVGDQFSAMFRRKAFLHWYTSEGMDEMEFTEAESNMNDLVSEYQQYQEATVDDEELIYNDDIVAEEPME</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEZ2</accession>
    <name>G3AEZ2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial distribution and morphology protein 31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_57869</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the organization of the mitochondrial membranes and the global structure of the mitochondria. Also required for mitochondrial distribution and mobility as well as for the maintenance of mitochondrial DNA nucleoids structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MDM31/MDM32 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372209.1">
        <property type="nucleotide sequence ID" value="XM_007372147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEZ2"/>
    <dbReference type="FunCoup" id="G3AEZ2">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="GeneID" id="18872229"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57869"/>
    <dbReference type="eggNOG" id="ENOG502QQJG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016236_2_0_1"/>
    <dbReference type="InParanoid" id="G3AEZ2"/>
    <dbReference type="OMA" id="DDEHHEV"/>
    <dbReference type="OrthoDB" id="17678at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006873">
        <property type="term" value="P:intracellular monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900208">
        <property type="term" value="P:regulation of cardiolipin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012571">
        <property type="entry name" value="Mdm31/Mdm32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31068">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31068:SF0">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08118">
        <property type="entry name" value="MDM31_MDM32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="325" mass="38008" checksum="23DD1738DA87CC1A" modified="2011-11-16" version="1">MEVKGMRGVVDRTHVHWDPDDDATNYKNIYHPGDFEFDKFKMEDVLFTLKQPNNFRVFDVAIYNCELPKLRKHWLFYDFLNANVMSGSYDNSLFTIHKKQRLNDYIDGDTAKWKRVTRMRVDALNVDHLNTGLEGPFGWISNGRVDMIGDVMVPQDSDELTVKEIVSIIADSIKKEATRYKNPEVMEKHPDLHTRLTSDDYTDISKYFVLDLTIRLNNVRASVPFQTPELSYINYALIRPIVAYINSKNTFIEIHNRIVKNIQDFSGSWTIYDSLLMDDISEEVYDNFVDYVADEEERMTRMKKVAFWSFQLLAQCIMFGLGSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF24</accession>
    <name>G3AF24_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_57919</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MYG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372240.1">
        <property type="nucleotide sequence ID" value="XM_007372178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF24"/>
    <dbReference type="FunCoup" id="G3AF24">
        <property type="interactions" value="1221"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF24"/>
    <dbReference type="GeneID" id="18872248"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57919"/>
    <dbReference type="eggNOG" id="KOG2948">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051576_0_0_1"/>
    <dbReference type="InParanoid" id="G3AF24"/>
    <dbReference type="OMA" id="FHCDEVV"/>
    <dbReference type="OrthoDB" id="10265310at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901857">
        <property type="term" value="P:positive regulation of cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003226">
        <property type="entry name" value="MYG1_exonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11215">
        <property type="entry name" value="METAL DEPENDENT HYDROLASE - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11215:SF1">
        <property type="entry name" value="MYG1 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03690">
        <property type="entry name" value="MYG1_exonuc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="322" mass="36613" checksum="C58BEF741FEE313B" modified="2011-11-16" version="1">MVQICTHSGSFHADESLAVYLLRLLPQFSDSTLVRSRNPSDWEASDIVVDVSGKYDGVKYFDHHQREFNEVFNGNYKTKLSSAGLIYKHYGRDIIKHVLKLSEDKDVEFLYDKVYKEFIESLDANDNGINNYPKDATPKFHDKNITLPSMVSRLNPSWNESCTDADFDRQFLKSTELMGSAFLALLEGYGKSWLPAKSIVQDAFDQRFEVDKSGEILVLSQFCPWKEHLYAIEQENNAEGEIKFVLFKDSTDKWRVSTVSVTSSSFEFRLGLPEELRGLRDEELSEKSGVEGCIFIHAAGFIGGANSEESVLKLARLSLQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF39</accession>
    <name>G3AF39_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_53961</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alkaline ceramidase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373227.1">
        <property type="nucleotide sequence ID" value="XM_007373165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF39"/>
    <dbReference type="FunCoup" id="G3AF39">
        <property type="interactions" value="596"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF39"/>
    <dbReference type="GeneID" id="18871826"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53961"/>
    <dbReference type="eggNOG" id="KOG2329">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063293_3_0_1"/>
    <dbReference type="InParanoid" id="G3AF39"/>
    <dbReference type="OMA" id="IMFEPLR"/>
    <dbReference type="OrthoDB" id="187171at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046514">
        <property type="term" value="P:ceramide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008901">
        <property type="entry name" value="ACER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46187">
        <property type="entry name" value="ALKALINE CERAMIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46187:SF3">
        <property type="entry name" value="ALKALINE CERAMIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05875">
        <property type="entry name" value="Ceramidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="100"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR608901-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR608901-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="303" mass="35470" checksum="2322D472B7BEF00E" modified="2011-11-16" version="1">MFPFAIPYPPEQVHGYWGIATSTIDWCEENYVVSRYIAEAVNTVTNSVFIALASFAIIHAYRNKLEPRFLFSALGFLLVGIGSWLFHMTLQYHFQLLDELPMIYATCIPFWSVFAEFKTKKQSMYIGWGIFTAANLLTVIYLYLRDPTIHQAGYGLLNVLIILRSTYLKGKHVHDKVAGKQLDRTCVLGIGLFLFGYFLWNLDIHFCDFVRLTRRNWGMPYGFVLEGHGWWHIFTGAGVYCSLVYQEYLRCFLTGTEKFYEFKWVGWFPMINCIDKFGLERHRSVQKLAEQDAVYYEQYKKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFA1</accession>
    <name>G3AFA1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein PUT4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PUT4</name>
        <name evidence="8" type="ORF">SPAPADRAFT_131915</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372302.1">
        <property type="nucleotide sequence ID" value="XM_007372240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFA1"/>
    <dbReference type="GeneID" id="18869620"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131915"/>
    <dbReference type="eggNOG" id="KOG1286">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_12_1_1"/>
    <dbReference type="InParanoid" id="G3AFA1"/>
    <dbReference type="OMA" id="FWIFFPG"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF39">
        <property type="entry name" value="AMINO ACID TRANSPORTER (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="410"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="465"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="493"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="7">
        <location>
            <begin position="51"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="570" mass="63916" checksum="5A3736C28D0C0AE1" modified="2011-11-16" version="1">MTILEKKENITVDIEQLSEEKPVVTTKFDDFDDKIDVEQYGEIKRKLNARHVNLMIIGQSIGSGLFIGLSSPLTTSGSLSLFIGFLVWACLVIYPLMQAVGEMCSYLPIKGTFLHYSARWVDPALGFACTLIYLYTSLMFVCLEATAVASLISFWTDANPAIFITISLVTYLLVNLVGVNWYGEIEFSSSILKVILIMGLMFFSLISMCGGNPQHNAYGFQHWKEGGLVKPFLVSGPTGQFLGFWNVLIYAAFACGGPDLLALCASEMKMPRKSVGIAAKRSYIRIYLFYFGGIFFLNSLCASNDPTLLHAIDTNAVGAAASPWVIGIKNVGVRGLDSVVNVCILASAWSCGNGFFYGATRCAYSAALAGYLPRFFSKCLPNGAPIYCVIASVLVGLLSFLSVSNSTATVFNWFVNLATTGLLCTYLCIWWCYFKFKKVYPKQTGVQFKKDEYNYYLAPKLIHPYFTYFGLLLNILVLFFNGFWIFFPGQFSVANLFTSYFAPVLYICLFVFWKFFKKTHFRSDMEADITTGKEQIDLEEEADIEYEATRERSQNWFFVVGRKISHFCFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFJ8</accession>
    <name>G3AFJ8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_131716</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372399.1">
        <property type="nucleotide sequence ID" value="XM_007372337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFJ8"/>
    <dbReference type="FunCoup" id="G3AFJ8">
        <property type="interactions" value="671"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFJ8"/>
    <dbReference type="GeneID" id="18869601"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131716"/>
    <dbReference type="eggNOG" id="KOG2363">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048451_3_0_1"/>
    <dbReference type="InParanoid" id="G3AFJ8"/>
    <dbReference type="OMA" id="CYGPGIT"/>
    <dbReference type="OrthoDB" id="17948at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002738">
        <property type="entry name" value="RNase_P_p30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01876">
        <property type="entry name" value="RNase_P_p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="302" mass="34281" checksum="04105F31D755BF84" modified="2011-11-16" version="1">MPYDLNIPWPVDNYTSKPTPSQLTQLKNIISTNYTLGITHQVINYSITETVKIPINNANEINPIDITWLRNELVNFPKLRLFTRLTLIVNDSSKIQHLTKLQNHFDLIAIQPLTEKALQLTITNLDVDLISLNLSSRLPFFLKHKIIGSAIDKGIKFEICYNWLISGAIGYDGTHANVQLIKKNFFNNVLQLIRASRSRGLVISSGATQPLQLRNSNDILTILKTLGLDRSRAKACMTVNPERVLVNGRLRIKSYKQTISVNDNAKLLENEHENPNKKSDSLVYKRKLQDTDTGKLLKKVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFM8</accession>
    <name>G3AFM8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PQ-loop repeat-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_58148</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372429.1">
        <property type="nucleotide sequence ID" value="XM_007372367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFM8"/>
    <dbReference type="FunCoup" id="G3AFM8">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFM8"/>
    <dbReference type="GeneID" id="18872343"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58148"/>
    <dbReference type="eggNOG" id="KOG2913">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049047_1_0_1"/>
    <dbReference type="InParanoid" id="G3AFM8"/>
    <dbReference type="OMA" id="QMSLDIY"/>
    <dbReference type="OrthoDB" id="292213at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036258">
        <property type="term" value="P:multivesicular body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052241">
        <property type="entry name" value="SLC66/Scramblase_ANY1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14856:SF9">
        <property type="entry name" value="PQ-LOOP REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14856">
        <property type="entry name" value="PQ-LOOP REPEAT-CONTAINING PROTEIN 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="38870" checksum="FD6852C914A147C4" modified="2011-11-16" version="1">MPFLPDDYLPYLEYLPDLQKCANIFMTFTPLFSYGSTCYSIHRTKSSSGFSLDICCTMLMASILRVYYYMCSPFEITLLRQSIVMIFIQCILLKVGLKYRSQGYDPDFLTPMPVFQLELTSRVPRRMSSAHAMKHETYWMGDWLSSVKLIMRDCYKFTQGYLSVLFISALRFFDVYFQRPGHFWQWKHEKPYWVFVVSFMGFFATCTLFLHSNTTYASTIGILGLFIESLLPLPQILLLNRLKSVENFKVILLLSWLGGDCIKISYLLYGTNDISIIFILAGLFQMSLDIYIATQYIQFKYGKHEQDIPLQDRSIDDIVFSMLEEQKSPTDTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFN9</accession>
    <name>G3AFN9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_64220</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372440.1">
        <property type="nucleotide sequence ID" value="XM_007372378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFN9"/>
    <dbReference type="GeneID" id="18874964"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64220"/>
    <dbReference type="eggNOG" id="ENOG502RJ5A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_569848_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFN9"/>
    <dbReference type="OMA" id="ERETMDQ"/>
    <dbReference type="OrthoDB" id="4022350at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="473" mass="55237" checksum="EEE84740B313743F" modified="2011-11-16" version="1">MGFINPGKSLKKRKLRQSTSITTSASTSEVEYRHKSITNHLTFVDLPNCIIRRIFIEAGPGNNIPLMNKSLRRMLKFDKEYVSEGYWDNLSLVLDMVRKFYAFNLNERLDFDVITKKMEYYTRVISYYKTRCPQNINYKRLVRNHDILRRICLEYTKNAWVLLDKVLSNNFISNRLLDFFTSKYLVGASEESVCKFMTRAEVLFMQKLRLRVVRFKFAEMAMDSAQLANDADSTTIDYNASMEEIVKLVDEWNVNNRSEENIDNPRLSYYDDEKLPVKEIPYQFSFIFDDGFLVAPVDPPSMFRIPATILGNGINSIQKFQLVQRLLLLHPDSLGPVDVILSKTFRHLQPDHIHNFPYRELPVGSIIPQLLSHTQTTGPCFGDVIIDMFRLFDSYTKIDYSIYGDNVDGDTIVQDLSKGCFLLLAFYFTRHTNVDSRPFWTEAIKLRNIQLTQVLSTFEPNPEYDILHNFHRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFS5</accession>
    <name>G3AFS5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dilute domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_131201</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372476.1">
        <property type="nucleotide sequence ID" value="XM_007372414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFS5"/>
    <dbReference type="FunCoup" id="G3AFS5">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFS5"/>
    <dbReference type="GeneID" id="18869559"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131201"/>
    <dbReference type="eggNOG" id="ENOG502QRMC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019651_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFS5"/>
    <dbReference type="OMA" id="YAKDWNL"/>
    <dbReference type="OrthoDB" id="426293at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051020">
        <property type="term" value="F:GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15473">
        <property type="entry name" value="Myo5p-like_CBD_DIL_ANK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037986">
        <property type="entry name" value="Myo5p-like_CBD_DIL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052072">
        <property type="entry name" value="Vascular_dev_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027:SF6">
        <property type="entry name" value="DILUTE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027">
        <property type="entry name" value="DILUTE DOMAIN-CONTAINING PROTEIN YPR089W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="142"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Dilute" evidence="3">
        <location>
            <begin position="372"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="761"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="766"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="782"/>
            <end position="794"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="824" mass="94581" checksum="8BBCA18E958B2494" modified="2011-11-16" version="1">MNAWTDASIANISTPFVENNLTSNVTSLGNTSLPILNYSDPLLSKILTSKKALHVLESNLVNSDSANSSKTLIELLIASCDGDLVTLESIIRSSDININQLFPSESNGVSCLIYSICFNNYSVAESLLNNHNADPDVFDTIAHYTPLMWAVYFNQLDIVKLLLDHQADPYLSPKDDDKNAVSLVTLENLEIYDFFKSHGLLKQKKSGDDEIYGTSSFQGEDFEDELSTDLRMQTLSSSIIDEPEEVREDDDDNEHVLSQDVVLQQLNEFDYDKLLPNQFLKFSDQDIPVLLDYIFGLRSKSSYQHNTKLPAIIIFQLVRYAHLKVESPELTEFLFDCFTARLRTVTNTKSGAFNMALQEPQDATSAAGGAGDIVLLSYWLSSIQFLHFYFTRNKLYQTLPKFLQEIINITQSLIATLSFSINSRLNLLVDDCLINFTNLVDVSNVLYAKDWNLFKKNKTHPSTYDDILDMLFPPSETELMKPSPLKYIQVLGALDYVLKIHQVDNLLQVQTFSQVFYYINATIFNRLISTSKYNSRAKAIQIRLNISAIEDWLRSHNMKVYRPESLGIDIGHELKNLLEVGKDKSKNDPHYLQFYYNSLYNVGKNQLTPTIELLQWLQCMSSLQDEDSLVNTINQFDYINYYQLFKVANKLYKYEVDEPKLPKKCTQLLKRLSNEQGPKQIERGVLHYMTQATFLSKELYIYLNPNFIFAVSLPNLTELINNYGAGLGGVKVFRSKKYQPSLPISIIDDIDEILTENKNQHLHDEYDYDREDDEEEDEDNDEEKKPGQDFKGDQIFKQVQMPNSLLHKNWGDEENNNDFEVNPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG00</accession>
    <name>G3AG00_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LicD/FKTN/FKRP nucleotidyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58328</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372551.1">
        <property type="nucleotide sequence ID" value="XM_007372489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG00"/>
    <dbReference type="GeneID" id="18872404"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58328"/>
    <dbReference type="eggNOG" id="ENOG502SD1A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_767620_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG00"/>
    <dbReference type="OMA" id="IIMENPR"/>
    <dbReference type="OrthoDB" id="444255at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052942">
        <property type="entry name" value="LPS_cholinephosphotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43404">
        <property type="entry name" value="LIPOPOLYSACCHARIDE CHOLINEPHOSPHOTRANSFERASE LICD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43404:SF1">
        <property type="entry name" value="MNN4P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="1">
        <location>
            <begin position="56"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="1">
        <location>
            <begin position="176"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="361" mass="43218" checksum="69D7DBF80157EE53" modified="2011-11-16" version="1">MVDNTPRYFKYPWIITDIIQGRAHHLSFPFFKRYIGDRERHSILHHMVRSWFQFTQVHGFNTWINYGNLLGWTYNGINMPWDTDIDVQIPIAQLDRLARDFNQSIIMENPRYGNARYLLEIAPTYIRQGNGKNFIDARFIDINSGLYIDISALSRTGFHPPAEFHNKESDMLVHCKNWNWHSLDELLPLRHTYFEGASVYIPQNVSQILTYKYGTKSFTQTKYHNHNYQKDIQMWVPDYICKKSPKHRFNEFGQLTRSGACNSEMLMDEYDITKECTQRHQNLNQDLSNPKYYNIEDLPIFRKDAWDYYNDLNNNLVNHDRWYVQMEPPKRSSVKQHISNDNDEEEDDSEFFDQDVWVNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGA5</accession>
    <name>G3AGA5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_58457</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PET117 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372656.1">
        <property type="nucleotide sequence ID" value="XM_007372594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGA5"/>
    <dbReference type="FunCoup" id="G3AGA5">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGA5"/>
    <dbReference type="GeneID" id="18872456"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58457"/>
    <dbReference type="eggNOG" id="ENOG502S49F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161486_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGA5"/>
    <dbReference type="OMA" id="VHYVQEL"/>
    <dbReference type="OrthoDB" id="76305at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031568">
        <property type="entry name" value="Pet117"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28163">
        <property type="entry name" value="PROTEIN PET117 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28163:SF1">
        <property type="entry name" value="PROTEIN PET117 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15786">
        <property type="entry name" value="PET117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="87" mass="9970" checksum="1FB0C28809B2D31A" modified="2011-11-16" version="1">MSTASKVTFGLSCVFAGATFVYINYVHQLERDALRQGPIKDKARMQDKFNKKQLANEAEHREQTELREKLVKMQPLSSEIIRGEEKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGA7</accession>
    <name>G3AGA7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_58459</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAPRE family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372658.1">
        <property type="nucleotide sequence ID" value="XM_007372596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGA7"/>
    <dbReference type="FunCoup" id="G3AGA7">
        <property type="interactions" value="720"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGA7"/>
    <dbReference type="GeneID" id="18872458"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58459"/>
    <dbReference type="eggNOG" id="KOG3000">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041744_2_0_1"/>
    <dbReference type="InParanoid" id="G3AGA7"/>
    <dbReference type="OMA" id="WIKRFWD"/>
    <dbReference type="OrthoDB" id="2119228at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051233">
        <property type="term" value="C:spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030543">
        <property type="term" value="P:2-micrometer plasmid partitioning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007019">
        <property type="term" value="P:microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031116">
        <property type="term" value="P:positive regulation of microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904825">
        <property type="term" value="P:protein localization to microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000028">
        <property type="entry name" value="RP/EB family microtubule-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004953">
        <property type="entry name" value="EB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036133">
        <property type="entry name" value="EB1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027328">
        <property type="entry name" value="MAPRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03271">
        <property type="entry name" value="EB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140612">
        <property type="entry name" value="EB1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51230">
        <property type="entry name" value="EB1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="11">
        <location>
            <begin position="4"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="EB1 C-terminal" evidence="12">
        <location>
            <begin position="207"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="126"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="126"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="165"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW35246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="350" mass="38850" checksum="656C1FC802E0741F" modified="2011-11-16" version="1">MVVGESRSELLQWLNTTLDLNYSKVEQCGTGAAFCQLLDSIVGGVPISKVKFNGTSEYDYRHNWKILQSGFTKHNITKNIDVEKLIKCRLQDNLELLQWFRRYWLENKDYNDVNYDASAKRRVSAGPASASGSVSSSAGSANVRRPAKPFITSPRVPTPGQRRVSSTSSVGSASFRSDRATGTTTPTNAAPASGAALRSKVNQLSKELEESYQEIVQLRNEIQDNKISTESLQIERNFYFNKLQDIEMIVQHILEAKEHGKSYEFADLDIFKLSNKIQTILYATEDGFGEAPAPMDISAPVAESVTNDVFSDDIGKDYLATSTTAYSREDNGTSLHVAHKSVDMSDNESF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGJ7</accession>
    <name>G3AGJ7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translocon Sec61/SecY plug domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_58567</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372748.1">
        <property type="nucleotide sequence ID" value="XM_007372686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGJ7"/>
    <dbReference type="FunCoup" id="G3AGJ7">
        <property type="interactions" value="172"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGJ7"/>
    <dbReference type="GeneID" id="18872505"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58567"/>
    <dbReference type="eggNOG" id="KOG1373">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031763_2_1_1"/>
    <dbReference type="InParanoid" id="G3AGJ7"/>
    <dbReference type="OMA" id="QAYCHIK"/>
    <dbReference type="OrthoDB" id="420669at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071261">
        <property type="term" value="C:Ssh1 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005048">
        <property type="term" value="F:signal sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="entry name" value="SecY subunit domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019561">
        <property type="entry name" value="Translocon_Sec61/SecY_plug_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10559">
        <property type="entry name" value="Plug_translocon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="299"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="431"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Translocon Sec61/SecY plug" evidence="3">
        <location>
            <begin position="42"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="499" mass="54371" checksum="EB1D54134E035C49" modified="2011-11-16" version="1">MGGFRLLDLVQFFLPVLPEIESPVEKIAFDEKVVYTVGGAFIFLLGQLPLYGLVSNAQFAIVDPLYNIRSIFAMEKGTLLELGLLPVITAAFIWQVAAGLRLINANLGLRYDRELFQTGQKLTAFALAVVYAAGLIYSGYYDNVIRGYDPLKDSTPIGSYVLLFTQIVSWSWLVTLMVEIFDKGYSFGSGVLSFLTLQVATNTVADLIGIEVFKVFNSNKSESYGALMNLVRNFSLFSPKKNAATIWHAFTRIQLPNLNQFYISLLTIGGVILLQNYRIEIPIRSTKVRGMSNVFPIRLLYTGGLPVLFAFTVLANVQVFGYFSTVVLSKLGAPQLLVSLLGKFELNPTSNNLNLKTGILFYLSNSTSLLQTLLSPIKTVVYAFSIVVLSVWFANKWSYISGSSPKDIAKQFKDQGISISGKRDISIAKELARVIPVASVSGAFLLAGLAVAGELLGGLGKGVATVVGVSSAFGVLEEFMLEYQQSGGSQFTSALSGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGJ9</accession>
    <name>G3AGJ9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_132725</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372750.1">
        <property type="nucleotide sequence ID" value="XM_007372688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGJ9"/>
    <dbReference type="STRING" id="619300.G3AGJ9"/>
    <dbReference type="GeneID" id="18869687"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132725"/>
    <dbReference type="eggNOG" id="KOG0092">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_10_2_1"/>
    <dbReference type="InParanoid" id="G3AGJ9"/>
    <dbReference type="OMA" id="RFRAGPY"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000808">
        <property type="entry name" value="Small GTP-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="229" mass="25589" checksum="30E4F08A8BACC569" modified="2011-11-16" version="1">MSVIEDSIQPPPYKIVLLGESSVGKTSLVHRFTSNTFDVHIANTIGAAFITKLYTSHSDANRKVKFEIWDTAGQERYRSLTPMYYRNAKVALICIDLSNFESTFHTAKYWIQQLELNNSSNADDKPIEIHLIGTKYDLVQSQTTPKISQEDIETKVNELIESHGNIVKFHTTSSKEGTGIVELFEDIVVAIDDDFFKSYYDKAQSETDVQGGPIGSILNARHAATSGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGP3</accession>
    <name>G3AGP3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_130700</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372032.1">
        <property type="nucleotide sequence ID" value="XM_007371970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGP3"/>
    <dbReference type="FunCoup" id="G3AGP3">
        <property type="interactions" value="2055"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGP3"/>
    <dbReference type="GeneID" id="18869518"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_130700"/>
    <dbReference type="eggNOG" id="KOG1414">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080216_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGP3"/>
    <dbReference type="OrthoDB" id="295274at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14687">
        <property type="entry name" value="bZIP_ATF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051027">
        <property type="entry name" value="bZIP_transcription_factors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19304">
        <property type="entry name" value="CYCLIC-AMP RESPONSE ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="6">
        <location>
            <begin position="132"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="148"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="215" mass="24439" checksum="2A41CDCC2D4CD3B4" modified="2011-11-16" version="1">MSKNPDFLSFLHNPFDINSYPITNPPIFDSTMLLPITPDGIPRRRRISISNGQIGQIVNHEAYFEAIAVAPPQQSMQNQMQVIPPPAQVPAQQEQSKTQFAGVPPPNHQLIYNNEVIFNPNNGPIPGTAAWKKERLLERNRIAASKCRQRKKQAQMELQNNIDKLEAELKDRDLKIQKLQATLKIYNKVLLSHFENGDDISELKQVIEQSQSLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGX2</accession>
    <name>G3AGX2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_57698</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372057.1">
        <property type="nucleotide sequence ID" value="XM_007371995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGX2"/>
    <dbReference type="STRING" id="619300.G3AGX2"/>
    <dbReference type="GeneID" id="18872158"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57698"/>
    <dbReference type="eggNOG" id="ENOG502RQC0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_599945_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGX2"/>
    <dbReference type="OMA" id="RRDYIGM"/>
    <dbReference type="OrthoDB" id="4024719at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="444" mass="51042" checksum="D3C4051494C22784" modified="2011-11-16" version="1">MDNVPVARLTKVCDFPPGQSGRVNVNTPRVTACVLVLNVEKNVARNSPCSLYVTDFTFNPQTYSDLRPNRYIWDDYSVPLGKVLKISVFNRYFRDLDDEYELRTGQELGLNQWYLDIPEGQHQKTSFLRKLLILNVEIDLRMYLDSLECNSYSFNIADPTDKRKEVQDLFDRMVNELPLEIFQENQDIAGKLIPSTFLLRVLPQYSTRLYQQTYANARDSHQQSYNSTNNSYQPKVHYSNNSTQIRQHHNTQTIEEYHTFNDFVEDLEHHSDYISTPAPTMSHTKPVRVKKESSQPIYQIHQLLGLNADAPNQVYTVNARVIDTSPSDWSLLCGKLYGIKNGKPEVMDPAPLDLELILVDATSNDKAINCRNSLSVHLPGNSVMEMLGIHAVEEMYINVSSLYERFMKYTAKPHKLELCRKPVPLNGEETIDAWTILNPNVISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGZ0</accession>
    <name>G3AGZ0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_57714</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372075.1">
        <property type="nucleotide sequence ID" value="XM_007372013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGZ0"/>
    <dbReference type="GeneID" id="18872165"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57714"/>
    <dbReference type="eggNOG" id="ENOG502SBHF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031125_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGZ0"/>
    <dbReference type="OMA" id="HTSPNIA"/>
    <dbReference type="OrthoDB" id="2587563at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="464"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="363"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="470"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="493" mass="55487" checksum="22CE87BFCE9438BB" modified="2011-11-16" version="1">MSGVIISNLPPPLSGSTGTTATAYTIKLNNEIISHLKNCLHKGITAKLVVKGGNYSIKISESLEFPCMKSPENLNVDIYSIASASANKHVYNGRIAAKLNVITDTRKIKQYTANANLAVSSPKPLSRSSPSPSIIVSTSTRPHANIDYTAFRTSTGDPDIVKKFIYLLALGPICSSRIAEIMHYENLKSLLDEYAQVYDPRDSFIEDDVFPSQDLSDDKVKQDPQYILKDKTYKELRPWKWNYSAYERDLIIGNINHALTRLGYSQTHPLRRKLCDQDGDKDEKPTTPLNAERSSGLGGGMLISKNAFKRAATDSPLISHSTPPPPPPPPAPVIKTDSQKRKLSSSSTSSDEEPQKKLKLEFSSASSSSSSSSSEEDRPNPVPPPAKKLDYYTNLAIKFKFKYKEYESLYLRLKQESKKDQESKKELVKLFEMHQLLSQWKKLLWDYDRDQKQKVNVMNLNKHKVSRSTSRTERADNVKRSVSPPKRRKVLDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH66</accession>
    <name>G3AH66_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">CSC1/OSCA1-like 7TM region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_146813</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372908.1">
        <property type="nucleotide sequence ID" value="XM_007372846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH66"/>
    <dbReference type="FunCoup" id="G3AH66">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="GeneID" id="18870727"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_146813"/>
    <dbReference type="eggNOG" id="KOG1134">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002458_2_0_1"/>
    <dbReference type="InParanoid" id="G3AH66"/>
    <dbReference type="OMA" id="QKWFFAF"/>
    <dbReference type="OrthoDB" id="1076608at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022257">
        <property type="entry name" value="PHM7_ext"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF20">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN 75"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12621">
        <property type="entry name" value="PHM7_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="124"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="449"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="497"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="543"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="588"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="663"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="702"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="736"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="10">
        <location>
            <begin position="51"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="11">
        <location>
            <begin position="224"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="8">
        <location>
            <begin position="448"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="domain" description="10TM putative phosphate transporter extracellular tail" evidence="9">
        <location>
            <begin position="776"/>
            <end position="869"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW35496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="872" mass="100732" checksum="C2F35B41B252650A" modified="2011-11-16" version="1">MSTISSEVDYDYSALTKTYMSIFNSQRFSQFLNTTQTGSAHSSSGIDFGLFSKTLFTSLCFCIIQLTLFCLFRSVFNFLYQPRCFCVPVNERMECLPRGFLNWIIPTLKCSINNYLSLGLDAYFFVRFISVLSLFFLFSGTLNMVILIPINFTGSDSEFSASGLDKLSLSNIANTKVKRLNAHFIMGLLTIGFFHWLIIYELQSFLIIRQSYLLSSTHKSSVMARTLLISNVPTYLQQHDVLRSVLKTIPGGISNIWDVYDFERIDHEVQRAKYALKVLEEAQIAGLMKYYSSKKSYFCCQTPTPNGDTNKLEFFHDHKNQVYFYPPIYLGLVKIPKFDRSFRMKLPGWWRMFMFEKRVSKTDWAIHTLSECHQYIDQEKLKLANGQLTKHNKLFIEFKTQEGAYIAHQCLLSQTQGCLDKTLIEINPADVIWRNVSRNDGIACKFEKYLVTIIFICIIILYVIPVSLIGLVSQIPLLTQLMPFLKWIYHFPEEARATISGFLPSILLTILTEICMVTFRFLTYFKRRTTGSEVELDLQKWYFAFLFVQQFLVVTISSSVTVIFKQIIDQPTSIPVLLATNLPKSATFFFQYMCLRAFAFCGSNFLRINQLILTNTHYRRIDKTPRQKFSRLTNLPKIKWGTTFSVYSIYGCIGISYSIISPLISIFIIFFLSLSILYYKYALKYVNSHLNESETMGRLYPIALLHLYTGIYCLECCLIGVFFLSKDQNGAYPMRVQGWIMTGVLFLTIFANTLIYNRFIPHFSNLPILSDKIYRESSEKVESPVPEDSDYEISESSYVNHKLLYLHPAFKYEPPKIWLPQDPDGYAELQIQEIESMLDSLSGGVTEGAQIRFTKPFNSLKLTISEAPPDYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHC4</accession>
    <name>G3AHC4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kinetochore protein SPC25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_65264</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the NDC80 complex, which consists of NDC80, NUF2, SPC24 and SPC25.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Chromosome</location>
            <location evidence="11">Centromere</location>
            <location evidence="11">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the SPC25 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373672.1">
        <property type="nucleotide sequence ID" value="XM_007373610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHC4"/>
    <dbReference type="STRING" id="619300.G3AHC4"/>
    <dbReference type="GeneID" id="18875032"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65264"/>
    <dbReference type="eggNOG" id="KOG4657">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085127_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHC4"/>
    <dbReference type="OMA" id="FRMNLAD"/>
    <dbReference type="OrthoDB" id="4056921at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23784">
        <property type="entry name" value="RWD_Spc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.50">
        <property type="entry name" value="Chromosome segregation protein Spc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045143">
        <property type="entry name" value="Spc25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013255">
        <property type="entry name" value="Spc25_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14281:SF0">
        <property type="entry name" value="KINETOCHORE PROTEIN SPC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14281">
        <property type="entry name" value="KINETOCHORE PROTEIN SPC25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08234">
        <property type="entry name" value="Spindle_Spc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="10 11" id="KW-0137">Centromere</keyword>
    <keyword evidence="4 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="6 11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromosome segregation protein Spc25 C-terminal" evidence="13">
        <location>
            <begin position="161"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="40"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="232" mass="27470" checksum="C77EFAD61192178C" modified="2011-11-16" version="1">MSTLSTNRFNDLIDLQQLQRIAEDYSSHLERKVSSNQSKLQQERQAHIKLIEELRAHSRELSIKKSAIEQEEAKQRNVLESSVESLYTQRRKVDELLKKKKELSDSKNELELEINKLKSSINERETQLTEINKKLEAQLAWIPNQISDYERYTGLKFDALEETLIQFIFTSIDPNEYEREFSFKLRIDQAYSIEDTIPQLEDDVVKSLSNEFNRNRFEVALRKVREAFQKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHH6</accession>
    <name>G3AHH6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="2 10">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_49208</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 10">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the ribonucleoside diphosphate reductase large chain family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="2 10"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373724.1">
        <property type="nucleotide sequence ID" value="XM_007373662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHH6"/>
    <dbReference type="STRING" id="619300.G3AHH6"/>
    <dbReference type="GeneID" id="18871315"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49208"/>
    <dbReference type="eggNOG" id="KOG1112">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000404_1_0_1"/>
    <dbReference type="InParanoid" id="G3AHH6"/>
    <dbReference type="OMA" id="LYDYESC"/>
    <dbReference type="OrthoDB" id="3000483at2759"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005971">
        <property type="term" value="C:ribonucleoside-diphosphate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01679">
        <property type="entry name" value="RNR_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013346">
        <property type="entry name" value="NrdE_NrdA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039718">
        <property type="entry name" value="Rrm1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02506">
        <property type="entry name" value="NrdE_NrdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573:SF28">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="R1 subunit of ribonucleotide reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="11">
        <location>
            <begin position="9"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW34140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="843" mass="95871" checksum="EF320B3E67F5E869" modified="2011-11-16" version="1">MPSTIIITPQIEEGGLVEKEFDRDKLLELLQNLCFNLDTDHLELEAVVSDIARGLPDRIEIEKLYEFVAEALASRIVYHPDYAILAGRIEIKKFDSIVPYTFSENVKRLQAYRHPRVKNRPYSMLSEKYCDVVMKHAAFFDEVIDKSRDNELTYFGIKTLENSYLLQMNNQIAETPQYLFLRVAIGIHFDDLESVVETYDLMSQKYLIHASPTLYNSGTEFNYLSSCFLLAVKEDSIDGIYKTLHQAALISKASGGIGIHMHDVRANGSFIASTSGISNGLVPMLRVFNNTARYVDQGGGKRPGAFAMYIEPWHADIFDILDMRKNHGNEEQRTRDLFYGLWVPDLFMKRVKEDKEWSLFSPDEAPGLSDVYGDEFVELYEKYEREGLAISTIKAHKLWLAILESQTETGVPYMLYKDACNSKSNQKNLGTIKCSNLCCEIIQYSSPEETAVCNIGSLALPSYLKTAENRAGEEVLEFDFVKLHKVAKVLVKNLNKVIDVTRYPVPSAELSNKKHRPIAIGVQGLADLFLELRLPFDSPQAKHLNMQIFETIYHAAIEQSTELAFQEGPYESFEGSPASQGLLQFDLWNHKPSPLYDDWDTLKENIKNFGLRNSLLVGPMPTASTSQILGFNECFEPYTSNIYTRRVLSGEFQVVNKYLIKDLIDLGIWNSSMRNKIIMENGSIQNIASIPQEIKDLYKTVWEISQRVIIDMAADRGKFVDQSQSMNIYLKQPTFGKLTSCHFYAWEKGLKTGMYYLRTQAATRAIQFTVDAEEASKVDNVEVSPSTLKRKKYLEIDHVSLKRKREAYVTPDSQSREDPTDIYDSTPLVCDIVETGSCQSCSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHL3</accession>
    <name>G3AHL3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MED5</name>
        <name evidence="10" type="ORF">SPAPADRAFT_134767</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373761.1">
        <property type="nucleotide sequence ID" value="XM_007373699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHL3"/>
    <dbReference type="STRING" id="619300.G3AHL3"/>
    <dbReference type="GeneID" id="18869847"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134767"/>
    <dbReference type="eggNOG" id="ENOG502R1HB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012200_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHL3"/>
    <dbReference type="OMA" id="MVINICG"/>
    <dbReference type="OrthoDB" id="5322661at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014801">
        <property type="entry name" value="Mediator_Med5_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35784">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35784:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08689">
        <property type="entry name" value="Med5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="905" mass="104541" checksum="C04B7611CE9F9994" modified="2011-11-16" version="1">MSEVSLHKLVTKAINQRLPSKSFISLINQFNVKQPIHDNQYIHDLLELDNTPTLKQQRINETKILQIIELSLSSSRQCHQFWKNLGKLDNNVQVRYLTKINKTLLHCSKYDRDLLKQLINTEFIDYSLDYLNKLHDKTFTTSQKQVVNHIIFIVGSIIDNKLVKSEQENILPIQNFIIKLITILKNLKLGKLLSFLLLKSKTILTSDQIEHLSHSKIIKHDLQVQVKTEDNVINYTSIASLSITTLSTEKQSDFYKTKRTFWLIKIIGNFEFDNIANMFVNFMPSGSSPYTLVYEFIQTFITCYDLLKHGDFRRFNLKNFLVTRVPSILGFFKISDEDIQTVVENVFADHTELKNDFTKSLALNRMLPTDKYPNDVTIPELNLRQRFNEKLLNINSEFTSLEESGLIEFMHALPNDLQYSKSHQLELSSIIVEIINDLISGKDFEKLNRILLCIMNNIELLNLIMFNCERAYSVLYQLIDFIDSTQFNIDDDDENFQDTFTYFGVTVLAIILIVHHFKLDYSFSIKNSFILDYINNFYYGLCDNLTNQETDDEEQIVNYNNLLTDWINALFDDNNDGLSDELIKSVNIKQIYKLIPIVYQQAIIATSSGKLGYASLTNGLDYLSQLFLIPSTVSLISWLVKELNLLQKLEADNAHLKVLSEIIKSNTSGNSQEISVLIFKIILNIHGNNIYKTITKFKEWEGIPIAKEIITAVGTYNSETAISKDLNVCETIKLQLLNKGTTLPIDFIIEYIEKNKAQVIWFLIYEVYNFQKSNDEELKYFINLMVYLIIIYSPSTKALQGVGVENGTIGVTDKFVLTIQDHYSSIFDTKLEPKEEDLDMLNDDLFNDLPNTSSTSQKLLKLNSEISFKKSLLGEFSKIKSDNHSLFEKSIKILNDKILDELNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHU8</accession>
    <name>G3AHU8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_65418</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373846.1">
        <property type="nucleotide sequence ID" value="XM_007373784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHU8"/>
    <dbReference type="FunCoup" id="G3AHU8">
        <property type="interactions" value="466"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHU8"/>
    <dbReference type="GeneID" id="18875041"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65418"/>
    <dbReference type="eggNOG" id="KOG0721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014210_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHU8"/>
    <dbReference type="OMA" id="RAILHAH"/>
    <dbReference type="OrthoDB" id="1734229at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031207">
        <property type="term" value="C:Sec62/Sec63 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071256">
        <property type="term" value="C:translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046967">
        <property type="term" value="P:cytosol to endoplasmic reticulum transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24075">
        <property type="entry name" value="SEC63 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24075:SF0">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC63 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="115"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="602"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="636"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="605"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="667" mass="75216" checksum="A0E49071AA7830F7" modified="2011-11-16" version="1">MASSEYNYDESGETWPFFVLAILSVILIPLSIKYIYRIRDSSNPVIENQKIKGAITEDATSIGVANADSIKHFKRKQKSDKIFNKTLVFLILGWLAVLYVGKYVTKDADTSVLFDPYAILDVSFSSTESEIKSHYRKLSLKYHPDKLPRDLTQEAKDKMEQEYIKLTSAYKALTDQTMRENYLRYGHPDGEQPTTHGIALPQFLVEGKYSSIVIVGYFLLIGVLLPYVVGKWWNNVKSHTRKGLRVETAAQFTRKLTDKDPGKVVTPHDILDWVLESQEIKKSFPNLSVGEIKNLVELHLNRDFSNTAVENDKVELVAKLPFLISGLVDIAVVFRVHDVILAAEDLKKCIVQAVPINGRYQELLQLPFVDRETVINQPIKKLGKLLTLSQDEAKKVLGIKDDAQVKRALEVASHIPTLRILEAEFKLPGEEVIPPQSSVHLSVKFLVKSPRIKSCPEIDDKRLKDEETLDYLKNPLIVNDQQPQLPFSYTPFFPISFRNGWDVMLINQKDNKLVEDSTVYSLENIDLSDLEISQESWKEGKDVVIGTFKIPLPQPTGAIGTHSYRVIMKNNAYFGADVDVPAYLEVEHAPIKAELSSVKKKLLDDSDESDSDSDISDPEEDSLAGALAALRGDVKNKSNKIQELNDDDSDNESVFTDINTDTEDEAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHV2</accession>
    <name>G3AHV2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">NTF2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_59687</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NXF family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373850.1">
        <property type="nucleotide sequence ID" value="XM_007373788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHV2"/>
    <dbReference type="FunCoup" id="G3AHV2">
        <property type="interactions" value="532"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHV2"/>
    <dbReference type="GeneID" id="18873028"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59687"/>
    <dbReference type="eggNOG" id="KOG3763">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024991_1_1_1"/>
    <dbReference type="InParanoid" id="G3AHV2"/>
    <dbReference type="OMA" id="TEYKMES"/>
    <dbReference type="OrthoDB" id="25872at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042272">
        <property type="term" value="C:nuclear RNA export factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030621">
        <property type="term" value="F:U4 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071528">
        <property type="term" value="P:tRNA re-export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14342">
        <property type="entry name" value="UBA_TAP-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030217">
        <property type="entry name" value="NXF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005637">
        <property type="entry name" value="TAP_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10662">
        <property type="entry name" value="NUCLEAR RNA EXPORT FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10662:SF22">
        <property type="entry name" value="NUCLEAR RNA EXPORT FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22602">
        <property type="entry name" value="NXF_NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03943">
        <property type="entry name" value="TAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00804">
        <property type="entry name" value="TAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51281">
        <property type="entry name" value="TAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NTF2" evidence="9">
        <location>
            <begin position="143"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="TAP-C" evidence="10">
        <location>
            <begin position="376"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="272"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="347"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="427" mass="48300" checksum="572479762CC128F7" modified="2011-11-16" version="1" fragment="single">MSTTSKFFPALMKIANDLKLDVVSIDLSNNELNDLQTISTMATTFPKLQNLSLHNNNFQRIRVFESWKHKLNFLRELILTENPILNNVNELQSIKLELMRSFPRLVVLNGEVLRNEQALNLNLTFPFESTQPMFFQDDNIQNIATNFITNYLKLWDSNRADLMILYQNESQFSMQLDSAHPHLIEASSTTGTDFGYYLHNSRNLTRVSSIKARQAKLATGQEQIYKSFTQLPKTHHDLINKPEMYSMESFTYAPLNGIMIIIHGSFEETAQPDNLDSLNSSSNSGPRGRYGGGPSKKKVPLSKKSFDRTFLVIPGPNGSMIVASDILTIRPFSSTTPWNKPVQAVATPTVTPNQSPAPQGPPSTELPPEIKSRLNPVQQELLVKILLETKLNIQYGIMLCEQSNWDYNQCSINFKNSAPTLPREAFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHZ8</accession>
    <name>G3AHZ8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_59731</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the citrate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373896.1">
        <property type="nucleotide sequence ID" value="XM_007373834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHZ8"/>
    <dbReference type="FunCoup" id="G3AHZ8">
        <property type="interactions" value="1023"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHZ8"/>
    <dbReference type="GeneID" id="18873052"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59731"/>
    <dbReference type="eggNOG" id="KOG2617">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022049_2_1_1"/>
    <dbReference type="InParanoid" id="G3AHZ8"/>
    <dbReference type="OMA" id="VLEWLFK"/>
    <dbReference type="OrthoDB" id="8017587at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046912">
        <property type="term" value="F:acyltransferase activity, acyl groups converted into alkyl on transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006101">
        <property type="term" value="P:citrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.230.10:FF:000001">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.580.10:FF:000001">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="entry name" value="Citrate Synthase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010109">
        <property type="entry name" value="Citrate_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01793">
        <property type="entry name" value="cit_synth_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007128">
        <property type="entry name" value="PRK09569.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739:SF8">
        <property type="entry name" value="CITRATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="3">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR610109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="465" mass="51755" checksum="52570A01D64B4B7D" modified="2011-11-16" version="1">MSTFRSLQRSSKLTRSALTQVRTLATAEPTLKQRLAEILPEKAEEVKQLKKEHGKTVIGEVLLEQAYGGMRGIKGLVWEGSVLDPIEGIRFRGRTIPDIQKELPKAPGGEEPLPEALFWLLLTGEVPTEAQTRALSEELAARSALPKHVEELIDRSPSHLHPMAQFSIAVTALESESQFAKAYAKGVHKSEYWKYTYEDSIELLAKLPNIAAKIYRNVFHDGKLPAAIDPKLDYGANLANLLGFGGNSEFVELMRLYLTIHSDHEGGNVSAHTTHLVGSALSSPFLSLAAGLNGLAGPLHGRANQEVLEWLFKLREELNGDYSKEAIEKYLWETLNAGRVVPGYGHAVLRKTDPRYTAQREFALKRMPDYDLFKLVSNIYEVAPGVLTKHGKTKNPWPNVDSHSGVLLQYYGLTEESFYTVLFGVSRAFGVLPQLILDRGIGMPIERPKSFSTEKYIELVKNIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI19</accession>
    <name>G3AI19_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_49385</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373917.1">
        <property type="nucleotide sequence ID" value="XM_007373855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI19"/>
    <dbReference type="FunCoup" id="G3AI19">
        <property type="interactions" value="491"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI19"/>
    <dbReference type="GeneID" id="18871330"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49385"/>
    <dbReference type="eggNOG" id="KOG0661">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_176_2_1"/>
    <dbReference type="InParanoid" id="G3AI19"/>
    <dbReference type="OMA" id="HPCLVQI"/>
    <dbReference type="OrthoDB" id="2158884at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040020">
        <property type="term" value="P:regulation of meiotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="26"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="535" mass="61267" checksum="673D58015BE3306D" modified="2011-11-16" version="1">MYKTSIPLDIDSPPKNLPLESYKSKYQPISTLGNGSFGTVDLVKFKFHKFHLLKYHDDKKGTLLYPLEDSKYNLSNLVAIKTMKKRLPLLHDYKNVKEVKFILSVQSHPCLVQIYEMFIDDVRYQLHISMEALNQNLYQLIRSRRNIQFSSPTLKSILSQLLCGIKHIHKFNYFHRDIKPENILIISTQQFYGSKEGIPPYRKNDNFIVKLADYGLARHVTNLKPYTAYVSTRWYRSPEILLRQKSYSQPLDIWAFGAVAAEIANFTPLFPGSNELEQIWKILRVLGSPLVPETSNNKYVIPLGGYWKDAQMLAHNLGFVFPEETGLTIDEVIHNPNLGQLAEVIQSCLLWDPNLRPTAVEVSRMAYFKESVSLLDRYFDNDIPIQVTLNKFSGIPSNPGVSSNYTTAFNDMTTDTVGSSTKKILTPTQTKRSSNRKNVIHSDCDFTKFYDDPNATGEENYFSGYVAESDKIQPSSGNTNDIYNICHAWMNKENKVNISSPASDKEPRIEYKTSVVPNKPSFTRFSLESEEVMNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI38</accession>
    <name>G3AI38_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glutamate--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="3">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glutamyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_135245</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="3"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373936.1">
        <property type="nucleotide sequence ID" value="XM_007373874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI38"/>
    <dbReference type="FunCoup" id="G3AI38">
        <property type="interactions" value="714"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI38"/>
    <dbReference type="GeneID" id="18869876"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135245"/>
    <dbReference type="eggNOG" id="KOG1149">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015768_6_3_1"/>
    <dbReference type="InParanoid" id="G3AI38"/>
    <dbReference type="OMA" id="QAPRYDN"/>
    <dbReference type="OrthoDB" id="428822at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00808">
        <property type="entry name" value="GluRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000045">
        <property type="entry name" value="Glutamate--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00022">
        <property type="entry name" value="Glu_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004527">
        <property type="entry name" value="Glu-tRNA-ligase_bac/mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049940">
        <property type="entry name" value="GluQ/Sye"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033910">
        <property type="entry name" value="GluRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00464">
        <property type="entry name" value="gltX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43311">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43311:SF2">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="An anticodon-binding domain of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="13">
        <location>
            <begin position="48"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class I anticodon-binding" evidence="14">
        <location>
            <begin position="390"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="23"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF19269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW34352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="524" mass="60671" checksum="CF02C6AA5B72D048" modified="2011-11-16" version="1">MKSIRVVFARSYSKASIISRNKKPNPLLSNSTLTSDRKSSHHPTTPARTRFAPSPTGFLHLGSLRTALYNYLLAKNTGGQFLLRIEDTDRTRLVETAEENIYSTLKWCGLMIDEGPNEGGPYGPYRQSERREIYKKYADLLLERGLAYKCYCSKERLLELRESAKKLKPPTNVTYDRKCLTSQHERDEHEYVIRFKSPEKYDQFEDVLHGTLNLQPQYNQQDRRYDDFVIVKADGMPTYHFANVVDDHLMKITHVVRGEEWLPSTPKHIALYKAFGWRAPKFIHIPLLTSLQDKKLSKRSGDLNIYSLKEKGVLPEALVNFVALFGWAPVRENPGESVSEVMDLQEIVSQFSIDHLTKGNAKVSDSKLNYLNKEHFLRKLNRPEEFNKLVESYYPVFTEFTHGKYNKEYFTKVVKIVGPHIGSMSEIEQNHMYIFQEVAYSKDKIPSEHTIEIISQVVNDEALEFNSLFKRIMKDSEITRKEIFTSLRFAITGGSSGLSVHEYLELVGEQEFRNRLQQALDFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIE9</accession>
    <name>G3AIE9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Glutathione S-transferase omega-like 2</fullName>
            <ecNumber evidence="3">1.8.5.1</ecNumber>
            <ecNumber evidence="4">2.5.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glutathione-dependent dehydroascorbate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_59851</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Active as '1-Cys' thiol transferase against beta-hydroxyethyl disulfide (HED), as dehydroascorbate reductase and as dimethylarsinic acid reductase, while not active against the standard GST substrate 1-chloro-2,4-dinitrobenzene (CDNB). May be involved in cell wall organization and biogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-dehydroascorbate + 2 glutathione = glutathione disulfide + L-ascorbate</text>
            <dbReference type="Rhea" id="RHEA:24424"/>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="ChEBI" id="CHEBI:58539"/>
            <dbReference type="EC" id="1.8.5.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GST superfamily. Omega family.</text>
    </comment>
    <dbReference type="EC" id="1.8.5.1" evidence="3"/>
    <dbReference type="EC" id="2.5.1.18" evidence="4"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374003.1">
        <property type="nucleotide sequence ID" value="XM_007373941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIE9"/>
    <dbReference type="FunCoup" id="G3AIE9">
        <property type="interactions" value="115"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIE9"/>
    <dbReference type="GeneID" id="18873102"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59851"/>
    <dbReference type="eggNOG" id="KOG2903">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037263_0_1_1"/>
    <dbReference type="InParanoid" id="G3AIE9"/>
    <dbReference type="OMA" id="YQLFVSY"/>
    <dbReference type="OrthoDB" id="2309723at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045174">
        <property type="term" value="F:glutathione dehydrogenase (ascorbate) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03190">
        <property type="entry name" value="GST_C_Omega_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000162">
        <property type="entry name" value="Glutathione S-transferase Gst3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000038">
        <property type="entry name" value="Glutathione S-transferase omega-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047047">
        <property type="entry name" value="GST_Omega-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016639">
        <property type="entry name" value="GST_Omega/GSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419:SF6">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE OMEGA-LIKE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419">
        <property type="entry name" value="GLUTATHIONYL-HYDROQUINONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015753">
        <property type="entry name" value="GST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01206">
        <property type="entry name" value="Xi.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01148">
        <property type="entry name" value="Xi_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST C-terminal" evidence="17">
        <location>
            <begin position="178"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="14">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="14">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="133"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="151"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="16">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="16">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW34419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="335" mass="39110" checksum="B3DC8400B45131B0" modified="2011-11-16" version="1">MSTDNKPIEILKFADDKDGAYKRRPSSFREFISSKPGARFPAEAGRYHLYVSLACPWAHRTLITRYLKGLTSIIGLSIVHWHMDSQGWRFINKEELASLKQKDDVSLGTVDHLYNFERIRQLYFKAEPDYNGRFTVPVLWDKKEQTIVNNESSEIIRMLNTEFNSLLPSEYADVNLVPSELEAQIDEINSWVYDNVNNAVYKAGFATKQEVYEKECKNLFVHLDKVEAILKAKHEQNEKFLLGNQLTEADVRLYTTIVRFDPVYVQHFKCNIGTIRHDYPHIHEWLRLLYWKIPGFQQTTDFDHIKFHYTKSHIAINPHGITPLGPIPNILPLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIK1</accession>
    <name>G3AIK1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">D-lactate dehydrogenase (cytochrome)</fullName>
            <ecNumber evidence="9">1.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">D-lactate ferricytochrome C oxidoreductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">DLD1</name>
        <name evidence="14" type="ORF">SPAPADRAFT_59081</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(R)-lactate + 2 Fe(III)-[cytochrome c] = 2 Fe(II)-[cytochrome c] + pyruvate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13521"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.1.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.4" evidence="9"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373300.1">
        <property type="nucleotide sequence ID" value="XM_007373238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIK1"/>
    <dbReference type="FunCoup" id="G3AIK1">
        <property type="interactions" value="207"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIK1"/>
    <dbReference type="GeneID" id="18872763"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59081"/>
    <dbReference type="eggNOG" id="KOG1231">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017779_3_3_1"/>
    <dbReference type="InParanoid" id="G3AIK1"/>
    <dbReference type="OMA" id="RACNAYS"/>
    <dbReference type="OrthoDB" id="7786253at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008720">
        <property type="term" value="F:D-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000014">
        <property type="entry name" value="D-lactate dehydrogenase (Cytochrome), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.45.10:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2740:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF111">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="13">
        <location>
            <begin position="125"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW33716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="558" mass="61217" checksum="91434AD77C6C2911" modified="2011-11-16" version="1">MFKRSFLRGKQFPLVRPYTTTTRRSFNPALLFAGGTIIGGYTVYKLSNLPVALADTPSLASTLPLEQLTSPTYATNAEIDQAIQEIRAAFLANTPDVDESLLINSTEEELQGHSDTYFNSHHATKTQRPKYVVYPRSTQEVSQIMKICHKYSIPVVATSGKSSLEGHFIPTRGGISIDISSMSNVVQLNKTDLDVVVQGGIGWEELDDYLAPYNLLFSTDPGPGATIAGALACNASGTNAYRYGETFKNVLNLTVVLADGTIVKTKQRPRKSSAGYNLTSLFVGSEGTLGIITEATLRLNVRPTEESVIVVPFHSIADAANAVNDIVLTGVQMSAIELLDDKMMNVVNLSGETTRKWIEKPTLFLKIGGPNLDVLRATVDQLKKIAAEHNNVDFQFASDADERTELWSARKVALWSSINHGIEIDKDMQLWTTDAAVPISKLPQFLMETKADIDAHGLKNTLVAHIGDGNAHSFILYTPDERKIAETVVSNMVQRAVNYEGTCTGEHGIGFGKREFLLQEVGQIPVDVMRNIKLALDPKRILNPDKIFKIDPNEPEGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIP9</accession>
    <name>G3AIP9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UBX domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_59889</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374049.1">
        <property type="nucleotide sequence ID" value="XM_007373987.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIP9"/>
    <dbReference type="FunCoup" id="G3AIP9">
        <property type="interactions" value="1085"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIP9"/>
    <dbReference type="GeneID" id="18873120"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59889"/>
    <dbReference type="eggNOG" id="KOG2086">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029402_4_1_1"/>
    <dbReference type="InParanoid" id="G3AIP9"/>
    <dbReference type="OMA" id="REVLHCN"/>
    <dbReference type="OrthoDB" id="25887at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036435">
        <property type="term" value="F:K48-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030437">
        <property type="term" value="P:ascospore formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061025">
        <property type="term" value="P:membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031468">
        <property type="term" value="P:nuclear membrane reassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031134">
        <property type="term" value="P:sister chromatid biorientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.210:FF:000002">
        <property type="entry name" value="UBX domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.210">
        <property type="entry name" value="SEP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036241">
        <property type="entry name" value="NSFL1C_SEP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012989">
        <property type="entry name" value="SEP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23333:SF20">
        <property type="entry name" value="NSFL1 COFACTOR P47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23333">
        <property type="entry name" value="UBX DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08059">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00553">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102848">
        <property type="entry name" value="NSFL1 (p97 ATPase) cofactor p47, SEP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51399">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEP" evidence="3">
        <location>
            <begin position="177"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="290"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="371" mass="41168" checksum="7B7A7FEE5EE3F541" modified="2011-11-16" version="1">MSDQLISEFVSITNSSKYLAEQYLSRNNNDLMEAVEDYYANDGNSASTPTSNQFQNADNASGSGRLRTLRDLNDDGGDDSADEKDMNYFTGGEKSGLQVENPNKDRRKGGNSQNDQSIIDQIFQRAKEQTNQPDDRPSAGDEPQQPKFSGRGFKLGDGNEPSQEIADPNANIFRPTKVNREITFWKQGFTVGETGELHRYDDPRNASILQELNQGRVPMSILDVEFGQDVDVSVFKKTDEEWKPPSLSRKAAGYFGKGQRLGSPVPGEPIVTESSSPQKEVVSSAPSEPQGEGDSLVQIRFANGKKTAHKFNSSDAVTKVYDFVRNHEYNDPSKEFNLSHAFPVKPIEDTSDITVADAKLKNAVIVQRWKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIT4</accession>
    <name>G3AIT4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome c oxidase-assembly factor COX23, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_59936</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the assembly of cytochrome c oxidase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the COX23 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374084.1">
        <property type="nucleotide sequence ID" value="XM_007374022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIT4"/>
    <dbReference type="FunCoup" id="G3AIT4">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIT4"/>
    <dbReference type="GeneID" id="18873140"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59936"/>
    <dbReference type="eggNOG" id="KOG4618">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153383_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIT4"/>
    <dbReference type="OMA" id="NPENHRH"/>
    <dbReference type="OrthoDB" id="9971592at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051040">
        <property type="entry name" value="COX23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46811">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46811:SF1">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="139" mass="16436" checksum="77DCF2EC68A8C729" modified="2011-11-16" version="1">MVEPSHAPTESKSAPADSTPENLDKTLKRQQAIANDFSDDTVEKDKSAINFTKGDQFKFYPDNPESHRHKYKWSMKEPSKFYDPCEESRMASMNCLWRNQDDKYACQEFFDAYRECKKDFFAKKKKDRLQGKHGWGFWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIV6</accession>
    <name>G3AIV6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">beta-glucosidase</fullName>
            <ecNumber evidence="4 11">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_48884</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Glycan metabolism; cellulose degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="4 11"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373351.1">
        <property type="nucleotide sequence ID" value="XM_007373289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIV6"/>
    <dbReference type="STRING" id="619300.G3AIV6"/>
    <dbReference type="GeneID" id="18871278"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48884"/>
    <dbReference type="eggNOG" id="ENOG502QR4D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004542_2_0_1"/>
    <dbReference type="InParanoid" id="G3AIV6"/>
    <dbReference type="OMA" id="VVMESWI"/>
    <dbReference type="OrthoDB" id="416222at2759"/>
    <dbReference type="UniPathway" id="UPA00696"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.300:FF:000002">
        <property type="entry name" value="Probable beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1700:FF:000003">
        <property type="entry name" value="Probable beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050288">
        <property type="entry name" value="Cellulose_deg_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019800">
        <property type="entry name" value="Glyco_hydro_3_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715">
        <property type="entry name" value="BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715:SF2">
        <property type="entry name" value="BETA-GLUCOSIDASE F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00775">
        <property type="entry name" value="GLYCOSYL_HYDROL_F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 11" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type III-like" evidence="12">
        <location>
            <begin position="731"/>
            <end position="801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW33767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="814" mass="90167" checksum="2A8DA0C56D22F073" modified="2011-11-16" version="1">MSITEKVNLTTGTGWGSGPCVGNTGSIPRLGVPNLCLQDGPNGVRFTDFVTHFPSGLAAAATFNKGLMYLRGKAIGKEHKKKGVHIALGPTIGPMGLKAAGGRNWESFGADPYLQGVGGAATVEGMQDEGVLATARHFIGNEQEHFRQVGEWEENGWDKLESAISSNIGDRAMHEVYLWPFADVVRAGVASVMCSYNQVNNTYACENSYLLNFLLKEELSFQGFVVSDWGAQHSGVNSVLAGLDMTMPGEVFDDWLTGKSYWGALLTRSVYNGTISQKRLNDMVTRILAPFFAIESVALPTEEDVPNFSSWTHHTYGQEFPYQHFGPIVQKNWHVDARSEFSDNTALTVAREAIVLLKNSGHHLPIDKGSGIRRILVAGKGAGPDPNGFNCRDQRCVDGVLTSGWGSAAVNNPFVITPYEAIAQKARDRGIMIDYSSSVWDLDNVEDLADYADLAIVVVNTFSGEGYVYVEDNFGDRQNLSLWHNGDQLIETIADKCKKTVVVVTSTGPVSMEKWIEHKNVVAVIFAPPLGQFVGQAIADVLFGDTNPSGKLPFTLARKKQHYVPIIDDLGGDRNPQDNFDRDIYLDYRFFDKHNIKPRFEFGFGLSYTSFKVCNLKITEINPPSEYLPYPQEYLPIYKLVEDDVCDPEDALFPHDEMDPAPGYIYPYLYNENVRSLDDQDDYEYPRGYDPDQPTAPVLAGGGLGGNPALWEVLYEVCAEVTNEGKLKGGYVSQLYIEFPSTILPSPPRVLRGFEKVFLEPGKSAKVYFNILHRDLSIWDPESQQWIIQTGTYKIYLSSSSRKVELCGEIDIGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIV7</accession>
    <name>G3AIV7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_64997</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373352.1">
        <property type="nucleotide sequence ID" value="XM_007373290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIV7"/>
    <dbReference type="GeneID" id="18875010"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64997"/>
    <dbReference type="eggNOG" id="ENOG502RW7Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_471712_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIV7"/>
    <dbReference type="OMA" id="KMARYEN"/>
    <dbReference type="OrthoDB" id="4020357at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="294"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="581" mass="67894" checksum="92381F5AC250EE69" modified="2011-11-16" version="1">MSSLLDKYQRSRKLGYNPRNNGESLYPTLSDHNDKNPVTTSSFSKPDRYNDVRPDRAYRDVSPIVRDRVPLRDPLKSWSSYNKEPAILSTRTTTTSERFAKNYSETKPFRSQLNEERYSRKLTNDDLKSKYNSTYFDSRSSKYGVDRVNQSIYASARARDYYTKRDTGLGSPIKSLNSPLDSTKRKSKSLYKVLGSTPIRSGDYRITKTPRKKHNVQESPGILSRLYKYFSKPGESDDVKILKNSARNVLHIDPVAEKKVAFHEPAESVRTRRQFEFDSEKDVVDEAIRHSKELSIEHQRFKALQQQYNRLEEELKHEISNNEKLVEQFIKDTDDLKIKYEQEKAQLQETIELLNAEIRTKDDKAKEIVQQLEYSEHEKYDLELRLDDLTKELDAQVEKNKIIRTEMELRERLKSKLENDTILHGIYKESAQIEEKIHKLIQEYPAEELPVSLLESNAANKYQRTEENINNALTDLKEFTLSLADTDFEPYDKFYDKVENFLNNAKSKIWNLIDPIAESSGSRNYYEKMLSNLFCLKRLNTLSYKSAQVKNLINDCKILDEYKTSGVDVSAIYQNITQETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIW5</accession>
    <name>G3AIW5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WH1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_70049</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373360.1">
        <property type="nucleotide sequence ID" value="XM_007373298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIW5"/>
    <dbReference type="FunCoup" id="G3AIW5">
        <property type="interactions" value="211"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIW5"/>
    <dbReference type="GeneID" id="18875302"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70049"/>
    <dbReference type="eggNOG" id="KOG3671">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015385_1_2_1"/>
    <dbReference type="InParanoid" id="G3AIW5"/>
    <dbReference type="OMA" id="EYNQDRK"/>
    <dbReference type="OrthoDB" id="8963340at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000147">
        <property type="term" value="P:actin cortical patch assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090135">
        <property type="term" value="P:actin filament branching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01205">
        <property type="entry name" value="EVH1_WASP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000281">
        <property type="entry name" value="Actin associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033927">
        <property type="entry name" value="WASPfam_EVH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00568">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00461">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00246">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50229">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH1" evidence="3">
        <location>
            <begin position="16"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="WH2" evidence="4">
        <location>
            <begin position="576"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="239"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="264"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="324"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="336"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="381"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="438"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="463"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="491"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="530"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW33776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="660" mass="70421" checksum="9ACDF180B43B2D9D" modified="2011-11-16" version="1">MAILTPNDKEKIKRAVPKANNKIIDATVARLYIAYPDPTKWVYTGLAGAIALVDDLVGHTFFLKLVDIVGNRGVLWDQELYVNFEYNQDRKFFHTFEIEDCLVGLLFEDTNDATHFFKRVTTRQKHGSSATVKNKNAIALKERAGPKGPSAPGPRGEFVDVNTAQRQRRSKGVFYYDDVPPPEWRSLYAELEAAGISEDMIADNREFIKDYIAKQGGPLVGLEPPIPRKYQRIVEHQPTRISSTASKHKKAPPPPPPPPPTGAAQVSATPESSHQGTPDVYSPSPEPQVPSDGSQHDSSPPVPEPAPALRFRVPPASALPPPVINTSLPSQPPVNQQVSSPFSPPGQQQQQQYQQPYQYHSAYQPQPQSPQQPQGPHKFGVPPPFQPQGGAPPPAPPSRANGVPPPLPPARTGNAPPPLPGRAGAPPPPPRAVSQGSAPPPPPPPRTARGQAPPPPPPRAHRTGSSQQFPPVQNQYQPMSPPPAMNQNRPIPVPPPMHHQQPPPPPLPPMHQQQVPPPPPPMPQQQQQQIPPPPPPPPPMPPMGGAGAPPPPPPPPMPDMSSGGAPALPEATGDTGRDALLASIRGAGIGQLKKVDKSQLDKPSVLLSEARGETQSAPPAAGGGGGAPGQPATLADALSAALNKRKGKVAHSDNEDDDDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ31</accession>
    <name>G3AJ31_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RWD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_59973</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IMPACT family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374127.1">
        <property type="nucleotide sequence ID" value="XM_007374065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ31"/>
    <dbReference type="FunCoup" id="G3AJ31">
        <property type="interactions" value="405"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ31"/>
    <dbReference type="GeneID" id="18873160"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59973"/>
    <dbReference type="eggNOG" id="KOG3299">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045276_1_0_1"/>
    <dbReference type="InParanoid" id="G3AJ31"/>
    <dbReference type="OMA" id="HLMQVMD"/>
    <dbReference type="OrthoDB" id="69641at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140469">
        <property type="term" value="P:GCN2-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031333">
        <property type="term" value="P:negative regulation of protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23822">
        <property type="entry name" value="RWD_ScYIH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.30">
        <property type="entry name" value="Impact, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023582">
        <property type="entry name" value="Impact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001498">
        <property type="entry name" value="Impact_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036956">
        <property type="entry name" value="Impact_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301">
        <property type="entry name" value="IMPACT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301:SF25">
        <property type="entry name" value="PROTEIN IMPACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01205">
        <property type="entry name" value="Impact_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="RWD" evidence="7">
        <location>
            <begin position="8"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="279" mass="32034" checksum="280C2E268DDA9724" modified="2011-11-16" version="1">MTVEELKDEIEAIDAIYPNSVTPLGPQIYTFKIPNHEYLAVQFTFPESYPEEIPQLLQVINDNRSRFTDTAYLERNIIEILNRVFVPDQVVLFEFLTELQEFFDKYVEEHPEKEVVPNPSPTLPPPPPPPKVITPEKPIKQVDPTKDWAKSDPILDRNSTFIGYARKVDSLEQAQTYLNELITDKKISRATHNISSWRIKTMDGVQYQDCDDDGETAAGGRLLHLLQMMDAWNVIVVVSRWFGGIHLGPDRFKHINSAARDVLIKGNFVNDTNNTKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJD2</accession>
    <name>G3AJD2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nucleoporin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_149697</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NUP186/NUP192/NUP205 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374175.1">
        <property type="nucleotide sequence ID" value="XM_007374113.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AJD2">
        <property type="interactions" value="186"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJD2"/>
    <dbReference type="GeneID" id="18870836"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_149697"/>
    <dbReference type="eggNOG" id="KOG1835">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002778_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJD2"/>
    <dbReference type="OMA" id="AYGFIEW"/>
    <dbReference type="OrthoDB" id="2019644at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021827">
        <property type="entry name" value="Nup186/Nup192/Nup205"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11894">
        <property type="entry name" value="Nup192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="486"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1922" mass="218679" checksum="77DA51FC3BB0FC7C" modified="2011-11-16" version="1">MNESMNWSCDVFANVYNIIKFQSPTAGEGGDLSQLDFSPIINDLLHILVTPTPLEESRTQLINETKPVTFSNGSQVKLNQPFIEISAVLAKELDLNEIASAELLYYAGDVSYKKGTSLGDAARFSYYSRLEYILNILGYLTIQKQLHLVITKESDFQILFDNLIKSFEKIYRLINNLHNMIDKQKVTSDINSLAFINSINFARTQLFNNHELLGKVLYGLVDQYFDKFGNLNNYKKMLQLISDHHEDGDILQIHFLPGVLQFIAKLLEGNDALVGEFHTHITTKISDDYRLVSIPTCGGDKDNDDIIDLSKSKISGFEIVTNFFFLSHFITWCKEEGTRTAKYDFKEHILKYMEYLISYGVMERILCYCAETSNAKTQQAFEYSNIYDFRSLLQRNFPKLTPSKFQYPGTQELLYASNMKPGFENIGKLLDVSYLTLNEDLNESLIAPFFQRFFSVFIVNAAIVLTQLRDLEEDFVLSSINRQQDEDSDEYETKTATTVSSRKTDDSYSDFDSNSRTRSAEKVGLDLDEVAQRADLERFYLAFAYTFNNRPELCNLFWSEEQQVSNDIVGFISWGLSNNTSPLITATFCILLSSLASGGNETAARIWEILVNNNNATMKKNDYSKISVDSLVDSLNYYIDSLNEQFELDLNDQLKLYQKKQEFLFSNNYSAQKEAEDANANRITIELAEDSIVFISGFMQLVSGIVKNLSIQNERGREIKNIAYTRFQPIIKGFLKFDNLIKGGKILSVDVNAGTSNNNPKFIDLPTILVSDDSMVILINLILTLLGDFVDHSKDDDGQGNTIGYEIWRLVDRWLYFGLHDLPETTTATTSTSSADGFSRTSLSATRKRYTSKRNMRINQAFSVNLTHFSEIANFTTLIRKLLTPFNTNNQAFSKYSLLFPCDLGLGYRVNNQIGIWPYIEFLMLEVFGNSHNISNKKDRHLLQSEVIEILINSLEEIDWKFLSETAPQVIRKLDNIDNIFDSLIPGIQIDFAMFAKLHQSLAVLHYLFDNKFFNTLFKVIDIGVDEVNSSESSSELVAQVLKVLDKLLDVQPTYLRRLLPLLKTKDGITQPQKQAPAQPLGVNTSMSLILSTPRTIFDNVYYPRNIGSHGVDDFYEILLFHLATVVHFALYVGCNNDEISNSAISILQQIDNSKYFHSRKIANSVDPLLENNRLLTTFQSIDESIKIKYAFINKFESLEESSFKMKYRIIEFLLNNLNHQPNGKVATVAHFLLGFDFRGHNLSLGNQNNTLLKTLLRTLSVSLDLLSEIDYNNGNKYIIDIGPAKLSSLILEVLIKLVRDPISSVVTLNQLREYDDLFEKLINYQPKLDLSTIWCGVEFNGDLQVDGVNAFIESSLSVETFFAFINQRNLILQYLSLEFHHISSITKKEYYINLLINNKEFLNHAPKILSFLDVLNYSFKNFEMQKYESLDKKFNMLSILQQAKHKSDNEQEEYLDLSILNKIIKILCQGSNAITNDAKRAFSQEVMVEGNRINEFVIKYIVSNDLKEVQLKCLHSWCQLIEILITDNGLQSTDFLLQVLQIVIPKTNDYLETDISFSAEMISLCVLLFDLYEKQILNQSKGEEDFTLGMGRLIPLFQTCLTGILNSNSTPNLRSDLYVLCNKFLMKIFDKDSSFVLPMIRIIKSSDKKFFEIICNDAIYSEGSSRITSTLLLESLIHLGSSAGKANFILDSLVKNNSLLLLVRSIKRTDQMIKLCIKPGGGVTLDNLIFELTAFKSTLYLLIRVSQSKSGALQLIQSELFSVLRQSRFLSIDPDLGLNLNIKEGHDFKSITVNVLLDTPSTLNDLVDLSKIRKENTISYFEFLIPIFQLITTVLLAMGPTYKPGIMETKGLMESFNRLIVGVMKRDVLLESRKDNVAGAVAGATNVTGIYKEDSTQVQDLQKLVKLFTLIDSLVNSSVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJM0</accession>
    <name>G3AJM0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cdc23 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_59306</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373506.1">
        <property type="nucleotide sequence ID" value="XM_007373444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJM0"/>
    <dbReference type="FunCoup" id="G3AJM0">
        <property type="interactions" value="1063"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJM0"/>
    <dbReference type="GeneID" id="18872857"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59306"/>
    <dbReference type="eggNOG" id="KOG1155">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018320_1_0_1"/>
    <dbReference type="InParanoid" id="G3AJM0"/>
    <dbReference type="OMA" id="YIMETER"/>
    <dbReference type="OrthoDB" id="3168162at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007192">
        <property type="entry name" value="APC8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF10">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04049">
        <property type="entry name" value="ANAPC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cdc23" evidence="8">
        <location>
            <begin position="2"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="187"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="221"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="255"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="386" mass="45295" checksum="C20ED3C5EEBF1962" modified="2011-11-16" version="1">MAQANLYKSIKLFPFNWSCWQELITSFTSFEEAISFLSKIKNSKHLSSHIMVQFFEVVVLQEFYHQSPSLFTTLSQLQDLFPKFSFVKLQQFLISYHNLDYFTAEHIFDEILAQDPLRLDDLDTYSNMLYVMEKRSKLTYLAHFASSIDKYRPETCCILANYHSMKSEHEMAIMYYKRALLLDKNCLSAYTLIGHEFVELKNSHAAIESYRKAVDINAKDFRAWYGLGQAYEVLDMHLYALYYYQRATKLQSNDKRMWIAIGGCFEKIEQYEEAIKSFEKALTISTSADEQGNTLIDPHIAYRLATISEKLGNLQNTEKYMKLCFNQEFEIGITDDTCKARLWLARYSLNQKNFNYAYELAKDLQHGNSHDIEEARGIAREARNRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJS5</accession>
    <name>G3AJS5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_49064</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373560.1">
        <property type="nucleotide sequence ID" value="XM_007373498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJS5"/>
    <dbReference type="GeneID" id="18871299"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49064"/>
    <dbReference type="HOGENOM" id="CLU_1897515_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJS5"/>
    <dbReference type="OrthoDB" id="10415162at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="134" mass="15356" checksum="A002867467CB4617" modified="2011-11-16" version="1">MTDIKPETNSDIEAYLKEKGILTESYDDPEFNKLIQELTTHFNWDKLEECFAFALTEYAKSIEALQERSASLIRRQSAWSEVLCAVDRDKSVARIQVCGDHIQNKQAKLNVMKEQLKKAIGSIRDHSSSMEKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK03</accession>
    <name>G3AK03_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_59476</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373638.1">
        <property type="nucleotide sequence ID" value="XM_007373576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK03"/>
    <dbReference type="FunCoup" id="G3AK03">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK03"/>
    <dbReference type="GeneID" id="18872932"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59476"/>
    <dbReference type="eggNOG" id="ENOG502QRJ1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1128958_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK03"/>
    <dbReference type="OMA" id="WLRLNFS"/>
    <dbReference type="OrthoDB" id="5581259at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051645">
        <property type="entry name" value="PER33/POM33_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703:SF3">
        <property type="entry name" value="ABR032WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="273" mass="31731" checksum="3F1F4E9DAA012CB1" modified="2011-11-16" version="1">MSSNASANGSTTVLTNEATTTQPSKVDPKTSKDAIPNDEEDLAMMKKRKLVRCNKTTNPLKFKIWQVGHFLTLGFGSISLLFQFFWLPNKYYINSICYRLTFIGAIMAMLATFSKKFGLRYLPSPASMLSQQNFQYLILAAVWIVSFKSVFKIIPFYLLSILHIGNWKEVSIIQKESEFISSLIAYDELLLIVYLLLRTIFFRGGSGFQLVLFLIFYWLRILYNKETRRLFGAIIERLDYEMSKVKNEKVSHYWKKIKLSIQDRQEQDSVKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK05</accession>
    <name>G3AK05_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 12">Adenylosuccinate lyase</fullName>
            <shortName evidence="12">ASL</shortName>
            <ecNumber evidence="6 12">4.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">Adenylosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_59478</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = fumarate + AMP</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer. Residues from neighboring subunits contribute catalytic and substrate-binding residues to each active site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="6 12"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373640.1">
        <property type="nucleotide sequence ID" value="XM_007373578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK05"/>
    <dbReference type="FunCoup" id="G3AK05">
        <property type="interactions" value="740"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK05"/>
    <dbReference type="GeneID" id="18872934"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59478"/>
    <dbReference type="eggNOG" id="KOG2700">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030949_1_1_1"/>
    <dbReference type="InParanoid" id="G3AK05"/>
    <dbReference type="OMA" id="VQENAMK"/>
    <dbReference type="OrthoDB" id="406045at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03302">
        <property type="entry name" value="Adenylsuccinate_lyase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.60:FF:000001">
        <property type="entry name" value="Adenylosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000005">
        <property type="entry name" value="Adenylosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019468">
        <property type="entry name" value="AdenyloSucc_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172:SF1">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10397">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00998">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 12" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Adenylosuccinate lyase C-terminal" evidence="13">
        <location>
            <begin position="374"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00017058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="482" mass="54459" checksum="7C7BF004244D7BCC" modified="2011-11-16" version="1">MSDNDKYSTPLSSRYASEEMSKIFSLRNRFSTWRRLWLNLAIAEKEVGLSMITDEAIEQMKNHLEITDKEIEDAAKEEAIVRHDVMAHVHVFGNTCPAAAGIIHLGATSCFVTDNADLIFLRDAYDVLIPKLVNVIDRLSKFALEYKDLPVLGWTHFQPAQLTTVGKRATLWLQELLWDLRNMVRARNDIGLRGVKGTTGTQASFLSLYHGDHDKVEELDKRVVELLGFDTVYPCTGQTYSRKIDIDVIAPLASFGATAHKFATDIRLLANLKEIEEPFEKSQIGSSAMAYKRNPMRCERVCSLARHLSGLFNDAVQTASVQWFERTLDDSAIRRISLPSAFLTVDILLSTMLNITSGLVVYPKVIERRINSELPFMATENIIMAMVEKGGSRQDCHEEIRVLSHQASAVVKQEGGDNDLIERIKKTEYFRPIWDDLDKLLDPSTFVGRAPQQTEKFVKNDVANALKPFEKHITTENVALKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK98</accession>
    <name>G3AK98_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cell wall mannoprotein PIR1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_60896</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PIR protein family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375072.1">
        <property type="nucleotide sequence ID" value="XM_007375010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK98"/>
    <dbReference type="GeneID" id="18873623"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60896"/>
    <dbReference type="eggNOG" id="ENOG502QQD8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1027339_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK98"/>
    <dbReference type="OMA" id="IGCIVAN"/>
    <dbReference type="OrthoDB" id="4023156at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054508">
        <property type="entry name" value="PIR1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051153">
        <property type="entry name" value="Yeast_CWMannoprotein_PIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000420">
        <property type="entry name" value="Yeast_PIR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254:SF1">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22799">
        <property type="entry name" value="PIR1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50256">
        <property type="entry name" value="PIR_REPEAT_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012587598" description="Cell wall mannoprotein PIR1-like C-terminal domain-containing protein" evidence="8">
        <location>
            <begin position="16"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Cell wall mannoprotein PIR1-like C-terminal" evidence="9">
        <location>
            <begin position="184"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="271" mass="28183" checksum="AD05646C2A6C0888" modified="2011-11-16" version="1">MKLFNLIILALSVNAAPVVDSSFTTKTPTDVTRTAGGLVLSDGSFRTALFDFNGDLTLSVQTLDPNEKDIGPAPSGSLNVGQVSDGQVNQISDGQVNAANQKPDGQVNAVSQISDGQVNAANQKPDGQVNAVSQISDGQVNAASQISDGQVNSNQAQDGQVTASGGFSSVCVSGNSLIVKLSNGILTDSHGRIGSIVANHQFQFDGPPPQPDSLFAAGWNIYPSAQGPLLALGGQYIFWRCAIQGTTFNLYDASIDNKCEKVVIHLFAVSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKB4</accession>
    <name>G3AKB4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arginyltransferase</fullName>
            <ecNumber evidence="2">2.3.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_137606</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the R-transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.8" evidence="2"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375089.1">
        <property type="nucleotide sequence ID" value="XM_007375027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKB4"/>
    <dbReference type="FunCoup" id="G3AKB4">
        <property type="interactions" value="633"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKB4"/>
    <dbReference type="GeneID" id="18870060"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137606"/>
    <dbReference type="eggNOG" id="KOG1193">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020349_2_2_1"/>
    <dbReference type="InParanoid" id="G3AKB4"/>
    <dbReference type="OMA" id="TCQEYDE"/>
    <dbReference type="OrthoDB" id="74183at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004057">
        <property type="term" value="F:arginyl-tRNA--protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030700">
        <property type="entry name" value="N-end_Aminoacyl_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007472">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007471">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367">
        <property type="entry name" value="ARGININE-TRNA-PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367:SF1">
        <property type="entry name" value="ARGINYL-TRNA--PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04377">
        <property type="entry name" value="ATE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04376">
        <property type="entry name" value="ATE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-end aminoacyl transferase N-terminal" evidence="5">
        <location>
            <begin position="15"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="N-end rule aminoacyl transferase C-terminal" evidence="6">
        <location>
            <begin position="156"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="481" mass="55968" checksum="69E44051D132C880" modified="2011-11-16" version="1">MPIVMDTPRNLSERRCGYCHGSKPDYFALESVKNHHDSNTHSQSVILGTSVLSMSSKDYDELINMGFRRSGIFLYKPDLLRTCCRQYTIRTRFDYLKLTKEHRKVINRFVKEICPEMVPNHEPKKGIAFDFSKLIKAQQKSTRFRTRFEPSGFSQEKYELFKKYQISVHGDKPEEVEEKSFRRFLCDSPFPMSEVTGNEQQFAFLQNWVKNWQPDQENPTSPNRRIGPTHELYYLDDKLIAFSVLDFLPTGVSSIYFVWDPDYAHLSLGTLSGLKEIQMCHELNLGYYYLGYYIDDCTKMKYKAKFGGEVLDVCNEVYFPMDMVKPYMANGRLFVIGDKEENVNHELELDNDGHPVDYTESPFFQRPLINIAESLYNSPDIKEAARMAGELLKRKYQIAHSAFEPNNIPLIAPGIIPLTQILTWLQTGIIDNNFQVKIFYSYENITDSFIWKELTAEERAIVVDCIRLFGLDKVQNGLIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKJ3</accession>
    <name>G3AKJ3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_151953</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375113.1">
        <property type="nucleotide sequence ID" value="XM_007375051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKJ3"/>
    <dbReference type="FunCoup" id="G3AKJ3">
        <property type="interactions" value="1272"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKJ3"/>
    <dbReference type="GeneID" id="18870914"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_151953"/>
    <dbReference type="eggNOG" id="KOG0926">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001832_0_1_1"/>
    <dbReference type="InParanoid" id="G3AKJ3"/>
    <dbReference type="OMA" id="EAIHNNS"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17982">
        <property type="entry name" value="DEXHc_DHX37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003770">
        <property type="entry name" value="ATP-dependent RNA helicase DHR1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000637">
        <property type="entry name" value="ATP-dependent RNA helicase DHX37/DHR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF99">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="433"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="667"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="188"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="716"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="80"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="196"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="249"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="263"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW33598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1231" mass="140629" checksum="612B0C8BEBD6B03C" modified="2011-11-16" version="1">MGKYRKRFNEKARAGMLAKQAALKKARNKSYYREQEPDQEQEPSETTTDATTIFNNEDSNTEILKPVTEEEKLERKRVLEETLYHENAQESKLSRSKKKRLDKYIEHQLKREEKKILLEKLSHTKIDTSKLSGLKELGKGKLTKREEMIEALELEQQGRGDERTKEILYEEREVKDYDEMSDDELRATFGVNKEESEVEDFDDDEPEEEQVKSTFVDNRPIGGFGTGFGFNNIPVIKKEASKKKYSWRKKVEQEEKKKLKLEDEQDFASSSEEEESEEESEEESEEVSEEEDEDQEEDEEADDASGEEEENSSEESEEESEEEEEEEQSKLMASKPKHSSIAKTFKQWAEQQIRTLEGRDNDLTATPQVSEEIKAQYAKQTIRDEDLASDPEDFIPINTKLDRKAFFVEIDRDDSIQQQRMQLPVFAEEHRIMEAIYHHDCVIICGETGSGKTTQLPQFLYESGFGSLNNDLYPGMIGVTQPRRVAAVSMAQRVSNELGNHGHRVGHQIRFDSTIKNEGTENGTALKFMTDGVLLREMMSDFLLTKYSCIIIDEAHERNINTDILIGMLTRVVKLRRKYHSEDSKYKPLKLVIMSATLRVSDFSENSALFKQAPPIINVQARQYPVSVHFNKKTNYDYLEEAFNKACKIHKKLPEGGILIFLTGQAEINTLVKRLRQEFPIKKKQYDEEVEVRLSENVQHEVEDVEFSVEVKDENYDDYEESGSDSEEEEGFEEAEEKEELGPLYVLPLYSLLPTKQQMKVFDTPPKGSRLCIVSTNVAETSLTIPGIKYVIDCGRSKERKYNQETGVQSFEIDWVSKASADQRSGRAGRTGPGHCYRLYSSAVFEEFFNQFSVPEILRMPFESIVLNMKSMGIDQITNFPFPTPPDRSSLKQAEELLVILGALDKDKRITDLGRKMSLFPLSPRFSKILIIGNQQGCLEYIIAIVSALSVGDPFLTEQELFTNKTEDQVRDLKSKFNKSRSLFSRLDSASDCLMLLSVVCAFDHVAKDNVDQFVQSNFLRFKIVEEIQKLRKQVQHLVETSTTGVITNSDKLAVPSKKQVSAIKQMIASGFIDQISIRGDLASSEVKITNKMNIINIPYCPVLRISQGPFVYIHPNSIINKRGELPPSYLVYQALTTKSQVSEDENPKVRMKPLVDITGKQLTNIAKNSKLISYSKPLGNPYGPKNITPTKRECYVVPRFGAAIGSGGIGWDLPAIKIIQEKRKGTWVNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKU0</accession>
    <name>G3AKU0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCAAT-binding factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_60319</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374509.1">
        <property type="nucleotide sequence ID" value="XM_007374447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKU0"/>
    <dbReference type="FunCoup" id="G3AKU0">
        <property type="interactions" value="648"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKU0"/>
    <dbReference type="GeneID" id="18873340"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60319"/>
    <dbReference type="eggNOG" id="KOG2154">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015945_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKU0"/>
    <dbReference type="OrthoDB" id="10263185at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030692">
        <property type="term" value="C:Noc4p-Nop14p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027193">
        <property type="entry name" value="Noc4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455:SF0">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="CCAAT-binding factor" evidence="3">
        <location>
            <begin position="116"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="334" mass="38923" checksum="EA5299CA1722480E" modified="2011-11-16" version="1" fragment="single">ESLEQWKTSKDEQQLRTIVANFYSLIRHGLLYVEEGIEEQQTWIGKLPSVAYKTTFKTQYQKCIITILSYPLLPSQYKAILLILHKRIIPYMSQPQGLMDFLTDSYDVGDDAIVPILALNSLYELMKKYNLEYPDFYTKLYSLLTPELLYTRYRSRFFRLCDLFLSSTHLSANLVASFIKKLARLSVAASASGVVIIIPFIYNLLKRHPTCMIMLHNPDKAKSGDYVDPFDNNEKNPMNTRAIGSSLWELETLMTHYHPNIATLAKIFGEPFRKHSYNMEDFLDWSYISLLESESTRRYKTLAALEFEEFDMLFDEADKSEETNKRVLLEGWTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL25</accession>
    <name>G3AL25_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_150671</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374583.1">
        <property type="nucleotide sequence ID" value="XM_007374521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL25"/>
    <dbReference type="STRING" id="619300.G3AL25"/>
    <dbReference type="GeneID" id="18870874"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_150671"/>
    <dbReference type="eggNOG" id="ENOG502R01W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_298626_0_0_1"/>
    <dbReference type="InParanoid" id="G3AL25"/>
    <dbReference type="OMA" id="SKFCQSP"/>
    <dbReference type="OrthoDB" id="6022054at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033596">
        <property type="term" value="C:TSC1-TSC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032007">
        <property type="term" value="P:negative regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007483">
        <property type="entry name" value="Hamartin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15154">
        <property type="entry name" value="HAMARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15154:SF2">
        <property type="entry name" value="HAMARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04388">
        <property type="entry name" value="Hamartin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="540"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="600"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="921"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="978"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="692"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="605"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="620"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="930"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="940"/>
            <end position="955"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1006" mass="115712" checksum="F37E371D9AF2AA06" modified="2011-11-16" version="1">MPGSSRDLSKSLDQIFSEWSDDNSSPEQPAYTELFSVISQYLDKHNTLASLRQQSVNDELFRVYDKYIMPLQSLPRECMFLDVLTALSPVLSSKEISLWVKSYLKPAVDSAGYDSMFVEKARQFIKKVCHGEETDDSDLESVRDDIGKVVIDQILSINLSDDILEEALELKVSDADKDSQSYQERLRFIKSNCNHILQNYGVSKTKRYCELLNDHITIPSQRLETLLLLSSLVNSKTLAVPDIINMPVFPNVLKCAMFDFSETVIHTALTILTMLIPQVSTSMSYYLPDLFMIYIRITTWEEFNSIPNRFQLLSEYLNELKIDWDILVDNEETSLTFNYPHFYVLLYGLFPFNFCKLSQAPYTYLKLNKPSILTLKYLNKLEDIIELPTSLDLHIRKMTKSILQTFLMHPKFLNFDTSTLEQELEHPTSWIEGEITHEEIALACFSLNPDVMINLDSSSIYGRMLQRSLTSSQILCDLDSRISRGSSIDGLKESKYLQMKAFHNLNRKMSIIPTNLVINNKQPTHDVEKEDGVQFKEVKYETENTEDSIDSLDGSSRSPGDPLQELFHTHEKLYAPINKNHNLENDGLRRKSSSLINVRHELRLSRPTSSPTTTLETSSVFKESASTNGSNNDTLTSLPHTKSDGNMIDFYQRELLLFKNELEFSSYMKHLNKYNYMKLKLNKSGDSKTIEMENNEKKIDDLKESFEELNGILTSTKQSLSQAITEHEKERDQLITRLNEVQTDKEELVRKYETTMREHKLESQTLKELVDDIIPQKDFEIVSLQMKIKNLEQMSRELEDKLGRVGTNNNLQELEKKIGNQNEIINKLKSEMSLVTEKHNQVVNELSRSSEAYDSIIRKYEHKLASSKLNLNENLNSFTSQYERKIQELTTTILKYEHLLEEKNGKILQLSSSKPISIPSYGRKSSYNDFEQHTDRSHSSSESSSPPHTSAPIAIHHPHPPAPIHYQTQPQAIRNNSTISNASSSSQQVPIMRGRGGYQKRSKKLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALE8</accession>
    <name>G3ALE8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_50101</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374706.1">
        <property type="nucleotide sequence ID" value="XM_007374644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALE8"/>
    <dbReference type="FunCoup" id="G3ALE8">
        <property type="interactions" value="1152"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALE8"/>
    <dbReference type="GeneID" id="18871404"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50101"/>
    <dbReference type="eggNOG" id="ENOG502RQBX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114640_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALE8"/>
    <dbReference type="OMA" id="HSARTYH"/>
    <dbReference type="OrthoDB" id="1431247at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00298">
        <property type="entry name" value="ACD_sHsps_p23-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="4">
        <location>
            <begin position="64"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="66"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW33191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="179" mass="20814" checksum="74833ADA621366B3" modified="2011-11-16" version="1">MFSRFFDDDFDDFFFRPRRYTTQKPADYNPRQITQGDDISLTPWGGQSTLSRDFNDSFWRNFNAGKYFVGFDEDLKTTEEKDKYLVTFTQPEGKLNPEDVKIDFHKKQNELVISISHEEKDENRSTSRSFVSSRIFAKPINPDDIKADITGGSIKLVLPKVEPDKEEKPNVVSVKVNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALK3</accession>
    <name>G3ALK3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative quinone oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">QOR2</name>
        <name evidence="4" type="ORF">SPAPADRAFT_60591</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374761.1">
        <property type="nucleotide sequence ID" value="XM_007374699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALK3"/>
    <dbReference type="FunCoup" id="G3ALK3">
        <property type="interactions" value="389"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALK3"/>
    <dbReference type="GeneID" id="18873479"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60591"/>
    <dbReference type="eggNOG" id="KOG1196">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_29_2_1"/>
    <dbReference type="InParanoid" id="G3ALK3"/>
    <dbReference type="OMA" id="YGIRTGM"/>
    <dbReference type="OrthoDB" id="809632at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05288">
        <property type="entry name" value="PGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF19">
        <property type="entry name" value="ENOYL REDUCTASE (ER) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase N-terminal" evidence="3">
        <location>
            <begin position="27"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="2">
        <location>
            <begin position="174"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="245" mass="26509" checksum="1197606128A09804" modified="2011-11-16" version="1" fragment="single">MSVPSVATSIIIKNCPHTDANCDFDQTDSTFDIKQFPIPDELPQNTCLVKTLLLSNDPHQRLSIHKNQDASRSFAAPLVPGDIMTSLGIVEIVKSNSAKYSSGQVVLGLTRWADYAVLSDCDLHQSINVPFEYPLEYYLSIFGITGLSAYFGLVDVGKVGRDSVVCVSAASGATGSMAIQIAKNVLGASKVIGISGSDSKCKFVEELGADICFNYHDDDWKLKFGQYLGDQFVDVFFDCVGGDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALM4</accession>
    <name>G3ALM4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
            <ecNumber evidence="6">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ARO3</name>
        <name evidence="8" type="ORF">SPAPADRAFT_55166</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the class-I DAHP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="6"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374782.1">
        <property type="nucleotide sequence ID" value="XM_007374720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALM4"/>
    <dbReference type="FunCoup" id="G3ALM4">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALM4"/>
    <dbReference type="GeneID" id="18871912"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55166"/>
    <dbReference type="eggNOG" id="ENOG502QPSU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030903_0_1_1"/>
    <dbReference type="InParanoid" id="G3ALM4"/>
    <dbReference type="OMA" id="DINTGLR"/>
    <dbReference type="OrthoDB" id="4699125at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000005">
        <property type="entry name" value="Phospho-2-dehydro-3-deoxyheptonate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006219">
        <property type="entry name" value="DAHP_synth_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00034">
        <property type="entry name" value="aroFGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009395">
        <property type="entry name" value="PRK12755.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE DAHP SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225:SF18">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE, PHENYLALANINE-INHIBITED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001361">
        <property type="entry name" value="DAHP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 6" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP synthetase I/KDSA" evidence="7">
        <location>
            <begin position="50"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="371" mass="40597" checksum="D6CD2EB160A8E187" modified="2011-11-16" version="1">MFITNEHVGDRTRMEDWRIRGYDPLTPPDLLQHEYPLTPESQKTILKGRDDAVNILNGKDDRLIIVIGPCSIHDPAAALDYAERLYKESEKHKGELLIVMRAYLEKPRTTVGWKGLINDPEIDGSFQINKGLRIARKLFVDLTSKLPIAGEMLDTISPQFLSDLFSVGAIGARTTESQLHRELASGLSFPVGFKNGTDGTLGVAIDALRAASHPHHFLSVTKPGVVAIVGTDGNQDCFVILRGGKKGTNYDEASVKETQEQLLKAKVVTDEKPGPRIMVDCSHGNSNKDHRNQPKVAAEVARQIAKGDKSICGLMIESNINEGRQDVPPAEQGGKAALKYGCSITDACIGWDSTIEVLDVLAQAVKTRRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALQ5</accession>
    <name>G3ALQ5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_71159</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374812.1">
        <property type="nucleotide sequence ID" value="XM_007374750.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALQ5"/>
    <dbReference type="FunCoup" id="G3ALQ5">
        <property type="interactions" value="839"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALQ5"/>
    <dbReference type="GeneID" id="18875362"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_71159"/>
    <dbReference type="eggNOG" id="KOG1167">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_118_2_1"/>
    <dbReference type="InParanoid" id="G3ALQ5"/>
    <dbReference type="OMA" id="PHEEFRN"/>
    <dbReference type="OrthoDB" id="10020333at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031431">
        <property type="term" value="C:Dbf4-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902977">
        <property type="term" value="P:mitotic DNA replication preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001100">
        <property type="term" value="P:negative regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045739">
        <property type="term" value="P:positive regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903468">
        <property type="term" value="P:positive regulation of DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060903">
        <property type="term" value="P:positive regulation of meiosis I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905263">
        <property type="term" value="P:positive regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905342">
        <property type="term" value="P:positive regulation of protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904968">
        <property type="term" value="P:positive regulation of spindle attachment to meiosis I kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031503">
        <property type="term" value="P:protein-containing complex localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14019">
        <property type="entry name" value="STKc_Cdc7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF23">
        <property type="entry name" value="CDC7 KINASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="98"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="511" mass="58804" checksum="D76A3AB3C136290B" modified="2011-11-16" version="1">MPQVLSSSSHEISMSSSSQFRKRLPSDRDDPFTDKKPKYELKESPFNVQRRKSDTSTKQQEENVDGGEEEEEEDEVSLEVLEEMHKLEECFPVLSTDYRLIDKIGEGTFSSVYKAEALNGKIRLGCDIWRSPPLKKKSNHHTKKRSPIVAIKQIYVTSSPNRIHNELSLLYTLTGSTYVAPLLDALRYQDQILAILPYYQHADFRDFYRDLPVKGIKKYLWEMFHALDYIHDKGVIHRDLKPTNFLYDPFKGKGVLVDFGLAEKLTTSTGTKNQCPCISKEKPINRSHSKRLNIKGAYPKTDSRPPRRANRAGTRGFRAPEVLLKCTNQTTKIDIWSAGIIGLSLLTRKFPLFNSPDDTDALVELVLLFGLEKLQKCAELHGCGLEITLPNVNQAHGNLIKLVHDFLKHEDDNGCFPHDSVIYDTLGLFNEEGDRFIKPQYTNLESLSQEERDLEYERVRRSNEDYKDHKHLMELLYGCFKMDPTKRLDSKQILKLPFFAELSQTEDDVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALW3</accession>
    <name>G3ALW3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Peptide hydrolase</fullName>
            <ecNumber evidence="15">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">SPAPADRAFT_136548</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in vacuolar sorting and osmoregulation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Vacuole membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the peptidase M28 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="15"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374237.1">
        <property type="nucleotide sequence ID" value="XM_007374175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALW3"/>
    <dbReference type="FunCoup" id="G3ALW3">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALW3"/>
    <dbReference type="GeneID" id="18869980"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136548"/>
    <dbReference type="eggNOG" id="KOG2194">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006412_1_0_1"/>
    <dbReference type="InParanoid" id="G3ALW3"/>
    <dbReference type="OMA" id="TPWPVTI"/>
    <dbReference type="OrthoDB" id="76293at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03875">
        <property type="entry name" value="M28_Fxna_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048024">
        <property type="entry name" value="Fxna-like_M28_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053975">
        <property type="entry name" value="PFF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053976">
        <property type="entry name" value="PFF1_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF58">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22250">
        <property type="entry name" value="PFF1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22251">
        <property type="entry name" value="PFF1_TM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6 15" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <keyword evidence="10 15" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="400"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="435"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="474"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="508"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="546"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="618"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="657"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="683"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="18">
        <location>
            <begin position="166"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar membrane protease transmembrane" evidence="20">
        <location>
            <begin position="438"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar membrane protease transmembrane" evidence="20">
        <location>
            <begin position="611"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar membrane protease C-terminal" evidence="19">
        <location>
            <begin position="711"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF22250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF22251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EGW32722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="975" mass="109797" checksum="DEA291D65BAA21FC" modified="2011-11-16" version="1">MSELNESTPPATRGTASSSDGVASNHVAVVKGNNNSPNIFIRFIRAVFGYRKTSLTLFVILTIAFTILLSYIDNNLDLTVSLPTDRLEVDILDSSWLDLQNIAKHEHTYGSHANDRVHDYLQTRIRDIIKEKSYIEYENDLNGDNKILFKSASGEQTTISYYESNNLLVRINGTDKNLPALLLSAHYDSVPSSFGVTDDGMGIASLLGVLSYFSDKSTQRPKRTVIFNFNNDEEFGLYGATAFLSHPWFEQIKYFLNLEGTGAGGKAILFRGTDFGIVKYFKNVRYPYATSIFQEGFNNHLIHSETDYKIYKEMGGLRGLDLAFYKPRDIYHTASDSIKNNNIKSLWHMLSNSIDFSKFVAGQVIDLDNESADESEKSSQDFASYASFLNYFFSVPVSTLIVVNTSLLVLVPLISLALLLIIFKYKKTWQVTTVNVLKFPLAAIASGVVMNLLVSDGFQKLNQYLPTTYPLLLVYTSVAILLLLFYVFLNGINLLFRSQKVLNHDEKLIVIIEISLFYWIALVYISRSLSYNRIGNDHTGEFPITILFIVQSVAAFLGLLGWSFTTPQTTDEEASQPLLEGNQESYGTEDPDDLTPQSSLLGDEDGFKGLVKYLGYDWSLQFLIIVPLTSLIVYNSGWLVLDGVNKSIQESLAAENLIYKIIQLFAFFWVLPYLPFVFKLNRIIVWGLTLFAASGVVLISFLEPFNIANPLKLRFIQKVDLTQSEQDSIVQVFGREGIFYDILADIPSVKESKVSVACENIVDGNQVCSYKSTLQPEIIPGAKDFRDYLQIEIVEDSSSRLPYGLLYGEIIIKAPENRMCNLQFDKKASHSGRDKFKDSPAKTVIVFADEPKNVSSVAHNTRMLPEGFSRDEDGNYLFKDVAGIDNLYLNKLDWKKNFRIGIQWLPNIIDSPDDEINKLGISIECFWADMTPVDGGKESSSAIPAYRELLHYSPNYVSWANRDRGLFSVSTHVQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALZ2</accession>
    <name>G3ALZ2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SWR1-complex protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_50235</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374860.1">
        <property type="nucleotide sequence ID" value="XM_007374798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALZ2"/>
    <dbReference type="FunCoup" id="G3ALZ2">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALZ2"/>
    <dbReference type="GeneID" id="18871421"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50235"/>
    <dbReference type="eggNOG" id="ENOG502QWM7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008595_1_0_1"/>
    <dbReference type="InParanoid" id="G3ALZ2"/>
    <dbReference type="OMA" id="MQKMCDC"/>
    <dbReference type="OrthoDB" id="4097064at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140849">
        <property type="term" value="F:ATP-dependent H2AZ histone chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037651">
        <property type="entry name" value="Swc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058986">
        <property type="entry name" value="Swc3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057558">
        <property type="entry name" value="Swc3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28108">
        <property type="entry name" value="SWR1-COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28108:SF1">
        <property type="entry name" value="SWR1-COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24707">
        <property type="entry name" value="Swc3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26242">
        <property type="entry name" value="Swc3_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SWR1-complex protein 3" evidence="2">
        <location>
            <begin position="30"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Swc3 C-terminal" evidence="3">
        <location>
            <begin position="511"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="Swc3 C-terminal" evidence="3">
        <location>
            <begin position="718"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="445"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="597"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="802"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="907"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="358"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="377"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="445"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="463"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="597"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="636"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="661"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="691"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="825"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="871"/>
            <end position="889"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="932" mass="105942" checksum="8B697D107B45028B" modified="2011-11-16" version="1">MPPRIRRRITSSKREEIEKEQEQLSRTIVRPFDVLNGLPVSFNPPPNGHYEDVLKQPLTIKDTGVLYRSLMKSRYYYVHVCPMFQLYWVKQSSYAKKLAEQDKPLPKSLKEDILINRKTILGNEVSARDVMVKLCDAAISLGPHTFEIRLFIAKDGRPEKMSKKEEKRLKALKEAEEKQKLLEGGTPEAKDQEKQPNSGQAELQTSKDELGIEQKQDNETKITEPEGKDKPEENKREENKSEENKSEENKSEGNKSEEIKPEEPESSPSKEQQNEESEHREIIKERSKEIEKDGKEEITDKHEEDIEREQESGFKEKGDADKSKSAEEQTAEIKKSVTDMKIPEKSASEADVTTIAGSGTSSSSLSPIPSREPSPPRTSSSTSTSTSKNDPNNMSSIENQIMIANLNFIARKDESLNSLMKIVASGKATAEQISTFQAYIKRAREMGPPPHDKEKKGKKVPKEKKPPKEKKEKKPKEPKAPKEPKPPKEPKVPKQPKEKKPKKLLKSQIPKDQRLTAFQERYIMDAILLFEFVENPNVRFMIPKNAICEVLPPSTMTNAEGEDNSDNRDILVSFLWIHNQNEVDEYESKLAEYNKKVKEEEEVKKAEEEKKRLEEEKLNGKQKEAEASGVGKETNENPDTEATNEPESIEAKSGETTQVKEEEEEEEENEDEDDEPEIKEERPYQAQSVPAKRRAPPRRSSRKKRKAPARKAKPVVPRKLEPPVEPEYKFTAFSFTIHGIPSRFVPIFTNSVKPLVEVQERISRILTVGTRISTFYLWYQMDGKLDEPLAEDIRMQLNQEEKKFTGIPTIHVEKTPSQPKKRKPKDKDGEGKKKAKKESDINNEDLVKVKKEGDQAEGNSADTEDGKSVEGTANPSENKSEEVSTPSNTVETAVVEENTAMEVDISEYNHNANSPVVEEIKDEAKNATQANV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM18</accession>
    <name>G3AM18_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane protein 135 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_137802</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM135 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374886.1">
        <property type="nucleotide sequence ID" value="XM_007374824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM18"/>
    <dbReference type="GeneID" id="18870075"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137802"/>
    <dbReference type="eggNOG" id="KOG1398">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012946_0_0_1"/>
    <dbReference type="InParanoid" id="G3AM18"/>
    <dbReference type="OMA" id="CLIRTRI"/>
    <dbReference type="OrthoDB" id="4021778at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026749">
        <property type="entry name" value="Tmem135"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031926">
        <property type="entry name" value="TMEM135_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12459:SF15">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12459">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 135-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15982">
        <property type="entry name" value="TMEM135_C_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein 135 N-terminal" evidence="7">
        <location>
            <begin position="310"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF15982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="479" mass="55966" checksum="1457475A5516B28B" modified="2011-11-16" version="1">MKISTLKGATSLYRNAANLPNEDIIQITNLTSKLLRTITAIVIRYIRLSIKNIFFSRSTLASFAFAYLFDVVPRLTNRIFYLVTHFEFREIPKEVTRILIAPFDATHFPVFVARLIATLNTFAPLCKQFISNFVEQDDSEVGFAGIFMAGFLSAMLNFPRFQKEKLKHDRYYTLDWTLILLTRMIETLVVTGTHSSKRFNIWKPIAEHGDVWLFIIANYIVMDTWVFKPWKLTPEYRQWISKASNLDEDITEGLRYIQQDKIDYSTGKQQVDSTNEHFMKLCVKYKRDIELGDISKQPKLPCTIFHQFRTDSCLMNSLVQFKYEFKFAFRIYTFLNLFAYVFIRRFRGSIKRLVWNSIRSSMFLGTASLIRWWTFCFIRNYHPPIQSQRFWDLAAIKLSNLLSGLSILVESHHRRKELSMFVIPKALGTFVDPTVNDFNIKIEIVAFSLSFATLIAFARTNPSRLRGLVGRALSYAIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM83</accession>
    <name>G3AM83_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_60733</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374896.1">
        <property type="nucleotide sequence ID" value="XM_007374834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM83"/>
    <dbReference type="FunCoup" id="G3AM83">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM83"/>
    <dbReference type="GeneID" id="18873543"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60733"/>
    <dbReference type="eggNOG" id="KOG2210">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012334_0_0_1"/>
    <dbReference type="InParanoid" id="G3AM83"/>
    <dbReference type="OMA" id="SSYWTEH"/>
    <dbReference type="OrthoDB" id="14833at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008142">
        <property type="term" value="F:oxysterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120009">
        <property type="term" value="P:intermembrane lipid transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000010">
        <property type="entry name" value="Oxysterol-binding protein homolog 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF184">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN HOMOLOG 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="355" mass="39526" checksum="41C60B1CE0268161" modified="2011-11-16" version="1" fragment="single">MTSTDSSSVSSASGTPSNDYSWTSFLKSIASFNGDLSSLTAPPFILSPTSLTEYSQYWAEHPDLFVDADLLTTSPNPTKEESEAMALKRMISVTRWFISTLKSQYCSRNETMGSEKKPLNPFLGELFVGKWESDDTHGEAVCLSEQVSHHPPVTAYAIKNKKHNVTLQGYNGITSSISTTSINIKQYGHAILEFQDKNESYLITLPPLHIEGLITAAPFVELEGKSYIQSSNGFFAVVEYTGRGWITGKKNTFKSRIFRDQFASSNKDNALVTIGGQWSGKSYIIKGNGNPGSKDELFYDAAASNAQHLTVKPIEEQHELESRRAWQKVAEAIRLADYNLIHQEKSKIENKQREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMX8</accession>
    <name>G3AMX8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bul1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61464</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375668.1">
        <property type="nucleotide sequence ID" value="XM_007375606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMX8"/>
    <dbReference type="STRING" id="619300.G3AMX8"/>
    <dbReference type="GeneID" id="18873908"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61464"/>
    <dbReference type="eggNOG" id="ENOG502QSAC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022027_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMX8"/>
    <dbReference type="OMA" id="FLQMFDF"/>
    <dbReference type="OrthoDB" id="4007955at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039634">
        <property type="entry name" value="Bul1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022794">
        <property type="entry name" value="Bul1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007519">
        <property type="entry name" value="Bul1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904:SF1">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04426">
        <property type="entry name" value="Bul1_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04425">
        <property type="entry name" value="Bul1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bul1 N-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Bul1 C-terminal" evidence="2">
        <location>
            <begin position="331"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Bul1 C-terminal" evidence="2">
        <location>
            <begin position="449"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="545" mass="63304" checksum="567EF17A76070343" modified="2011-11-16" version="1">MIANKNDKSDRRPVRIRKFLEMFDFYGSWNEAHINRLVTDFGNPYECPNIFDPIDGTFLAFGDKKTIFPNKVYKRIFTFRIPNNLLDSECNEHNLSKHVELPPTIGMSRWELAHFPDREHNKIKDFSLLDTSISYGVMARFIGKKSTWEKDFGKINTSAYSIDKRLINSKGDEYTILKELTNYVRVVQRTRIPTDNERLMKLLENKLMYNNLISRVKEKIEAGKQMIKSIENNEFNATVDIGRQLTETEIELAKCKQAYRRDAYRDIKAKKIVEKYDIFLPLMKNSITGTKNIGTLNLSTPKQEYCVCYIPPSRFREKPIDDIVPSWKFDIPLDLSVSFPLGSKNSTPPSIKMISAELAVSTIRSRRLPIPIEFNHDLIYNKQIENNRGFKDEDTFQNNIIIPFQKLSTELYTLCKTLGADNFKVEKRLVDDLKAICELEEKKLNLNVSDMKINNAKFNSKLIKWDIKDNTAKTSINVGVNLESLVLKGFEGSPTLMKSYDRFNLVPDFQSCFMSRLYHIKVTILLSNNDYVRIKVPVRIQKITT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN05</accession>
    <name>G3AN05_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Initiation-specific alpha-1,6-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61486</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375695.1">
        <property type="nucleotide sequence ID" value="XM_007375633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN05"/>
    <dbReference type="STRING" id="619300.G3AN05"/>
    <dbReference type="GeneID" id="18873916"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61486"/>
    <dbReference type="eggNOG" id="ENOG502QW2I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022381_5_0_1"/>
    <dbReference type="InParanoid" id="G3AN05"/>
    <dbReference type="OMA" id="QVACDHA"/>
    <dbReference type="OrthoDB" id="409543at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039367">
        <property type="entry name" value="Och1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834:SF1">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="31863" checksum="57423153003A843A" modified="2011-11-16" version="1">MWKVGLDDPDFPERFKEFHQTWLDNSPDYTHRVMSNDERDALVLKLYADIPEITHAYQIMPKTMHRCDFSSYLLLYALGGVYADIDTKLLKPISSWIPSQPSYLDKPIDLGLIVGLESDYHKKNWANLFARRVQIEAWTFLAKKGHPMLAEIIDSIVEHTSWREETGQLTALLGKDEGTDVLNWGGPGRLTDLVFKYLNNVLQTDFDNYEKLIDDEFLIGIKLPIVISDAMVLPLTCMQPGRKFLKSGNLDDPLAYIHHKSTGSWRKENKGKDTYVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN42</accession>
    <name>G3AN42_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61524</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375732.1">
        <property type="nucleotide sequence ID" value="XM_007375670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN42"/>
    <dbReference type="STRING" id="619300.G3AN42"/>
    <dbReference type="GeneID" id="18873936"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61524"/>
    <dbReference type="eggNOG" id="KOG4324">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_708170_0_0_1"/>
    <dbReference type="InParanoid" id="G3AN42"/>
    <dbReference type="OrthoDB" id="1748564at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070319">
        <property type="term" value="C:Golgi to plasma membrane transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21044">
        <property type="entry name" value="Rab11BD_RAB3IP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040351">
        <property type="entry name" value="RAB3IL/RAB3IP/Sec2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14430">
        <property type="entry name" value="RABIN3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14430:SF0">
        <property type="entry name" value="SEC2P DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="243"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="269"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="317"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="334"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="390" mass="44061" checksum="84A968F9028B09CF" modified="2011-11-16" version="1">METAIPTIPALPSTSTFLNRWQKGKNFWSSVCEGRVSIEPVKGTNESFKLAYRGSMSHVTPVAIRDSCSFCGESRNDNLEHARMHYFKILEPIGDDVIASYPLCNYCLIKLRNVCDLFAKLRVIKSNVFKLKPNSAFDDIATTTNTFGQFKRSTSGSIPKNGDESPVTTLSSTETLMNSQVQQIELDKVEESKLIKLYMMVLLVRLKIFWSKIGFWDNVDRISEINLDEIHYETFEYLIPKSESRASTADKNEGEQEEEQGAKVASETAKGETEDVDAGAHDDSRSDAEEFIDTQDKFEQSTVDEHAEQAPPANNENSHEQETKPEEESEPVESSESQDTQETQQERDEGEPGATEMYRSNSKKFRAKMDSDLDHTLEMLAENIEVNTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN45</accession>
    <name>G3AN45_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_138116</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375735.1">
        <property type="nucleotide sequence ID" value="XM_007375673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN45"/>
    <dbReference type="GeneID" id="18870098"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138116"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034127_0_0_1"/>
    <dbReference type="InParanoid" id="G3AN45"/>
    <dbReference type="OMA" id="YSTGVCI"/>
    <dbReference type="OrthoDB" id="4142200at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904659">
        <property type="term" value="P:D-glucose transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050820">
        <property type="entry name" value="MFS_Sugar_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48023">
        <property type="entry name" value="D-XYLOSE-PROTON SYMPORTER-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48023:SF4">
        <property type="entry name" value="D-XYLOSE-PROTON SYMPORTER-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="378"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="417"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="445"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="1"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="540"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="576" mass="64372" checksum="E80126526289B03E" modified="2011-11-16" version="1">MKNKIYNSYAIGVSISTFCLTCGMEMQTYLTAKSSKLFAAIFTNPSKIEDTILSTSNPLGAIFGSIICGQICHKFGFIRGFQVICLLWIIGIIIAFFSSTTWLLIIARVVKGVAIGFTYASVPIYIYETIPPNSRGRVLSIGIFCGASGNLITFSIYNLIEEFVKEIAPIWTRLYWSFGVVPVVISFGFTFLIPESPKWLATNSKWETAADVLQSIQLKNSSGLESKTKDERIKLGDKHFVIKEYTSGVCVKSCSLNRLFGKKYIKELVTAILIQFFLVFTFITKLQESFDCICNACQLLTVDTKLANLFDISMRILFACFPIVVLDILRRKDILVFAVIITTGIMIGFTILFLFFSYKTSAARINWQLQRVFFDQTASFLLALTVLADVIYTSILLPSCWLLTIETFSSSSRIQGWVVSTCIFWILETITSLVFPFLLQILNEWLFLVISIICIVGLGFSIKLAETKGKSGLDSDFVLDSKSILQLSPTKSIQKELPRFAQPIEKVQANRAISTYRHNTINRFGSKNASLGSKRVNEIVQIQGESENKENQTTGASSEEEEGTSQSYSPASLEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN75</accession>
    <name>G3AN75_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 14">Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase</fullName>
            <ecNumber evidence="3 14">2.7.4.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_50530</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Bifunctional inositol kinase that acts in concert with the IP6K kinases to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. PP-InsP5 and (PP)2-InsP4, also respectively called InsP7 and InsP8, may regulate a variety of cellular processes, including apoptosis, vesicle trafficking, cytoskeletal dynamics, and exocytosis. Phosphorylates inositol hexakisphosphate (InsP6).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1D-myo-inositol hexakisphosphate + ATP = 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:37459"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:74946"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:37460"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate + ATP + H(+) = 1,5-bis(diphospho)-1D-myo-inositol 2,3,4,6-tetrakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:10276"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58628"/>
            <dbReference type="ChEBI" id="CHEBI:77983"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:10277"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
            <location evidence="1 14">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the histidine acid phosphatase family. VIP1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.24" evidence="3 14"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375194.1">
        <property type="nucleotide sequence ID" value="XM_007375132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN75"/>
    <dbReference type="FunCoup" id="G3AN75">
        <property type="interactions" value="540"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN75"/>
    <dbReference type="GeneID" id="18871452"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50530"/>
    <dbReference type="eggNOG" id="KOG1057">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000914_3_1_1"/>
    <dbReference type="InParanoid" id="G3AN75"/>
    <dbReference type="OMA" id="IQERWCC"/>
    <dbReference type="OrthoDB" id="18042at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033857">
        <property type="term" value="F:5-diphosphoinositol pentakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052723">
        <property type="term" value="F:inositol hexakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052724">
        <property type="term" value="F:inositol hexakisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000830">
        <property type="term" value="F:inositol hexakisphosphate 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000831">
        <property type="term" value="F:inositol hexakisphosphate 6-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052846">
        <property type="term" value="F:inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 1-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052843">
        <property type="term" value="F:inositol-1-diphosphate-2,3,4,5,6-pentakisphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052845">
        <property type="term" value="F:inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071545">
        <property type="term" value="P:inositol phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051516">
        <property type="term" value="P:regulation of bipolar cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110162">
        <property type="term" value="P:regulation of mitotic spindle elongation (spindle phase three)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000036">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11950:FF:000002">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037446">
        <property type="entry name" value="His_Pase_VIP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040557">
        <property type="entry name" value="VIP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750">
        <property type="entry name" value="DIPHOSPHOINOSITOL PENTAKISPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750:SF9">
        <property type="entry name" value="INOSITOL HEXAKISPHOSPHATE AND DIPHOSPHOINOSITOL-PENTAKISPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18086">
        <property type="entry name" value="PPIP5K2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="VIP1 N-terminal" evidence="18">
        <location>
            <begin position="173"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp fold RimK-type" evidence="17">
        <location>
            <begin position="387"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="85"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="918"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="587"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="920"/>
            <end position="937"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF18086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW31918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1141" mass="129227" checksum="0B5331B64F4AB06E" modified="2011-11-16" version="1">MSDNTRPIPVPKVLNASNQDSIDPKDVSVESSKTVSPLSLSLGEQSSLVDRLPPTAKRISSKSMSSPATERAIASIAPMLQSFTPKTSAEDSASRLNSTSVSSGNNSTENVNEGENGPDTLTVENLIQNTEQLKLDTGKLFKTMSNTSTTSRKSVSVSKDVVNTAPTLPKIGKIGVCAMDAKVLSKPCRKILNRLIENGEFDTVIFGDKVILDESIENWPTCDFLISFFSTGFPLDKAISYVNYRQPYFINDLVFQKAFWDRRVVLAILEHANVPTPKRLEISRDGGPHLDNLLLTKMKAIGISDDFLEKLTNQAEPDWEMVDEDTLRVGDQVLEKPFVEKPVDGEDHNVYIYYPKSTGGGGRRLFRKIGNKSSEFDPNLTSPRTEGSYIYEKFMDTDNFEDVKAYTVGPEFCHAETRKSPVVDGIVRRDTHGKEIRYITELTPEEKTIAQNVSSTFKQTVCGFDLLRVNGKSYVIDVNGFSFVKDNNEYYDMCAKILRDLFIEAKKSRDYLKTTIPPSTKLLHKSQFEEKEQKWRFKGMVNIIRHADRTPKQKFKYSFRSPLFISLLKGHLEEVIIRAVPDLQVVLETVKIAIDKQLEDLKKLKQLQNALEKKMNFPGTKIQIKPSLNKENPEIVDKVQLILKWGGEATHSARHQAIDVGDQLRQNIKLLNRDALKEIKVYTSSERRVITSAQYSTMALLGLDPETETLADDFLIVRKDLLDDSNAAKDLMDKVKKKLKPLLRQGAEAPPQFTWPPKMPQPFEVIKRVVELMNFHQQIMNYNFETKDVQEFQTNWCCGEDPFLFKERWDKLFQEFISVEKTHPSKISELYDTMKYDALHNRQFLQKVFSFDPNDETLLTRLRETCGDTVNSSGLVSEYPINILAMNNFKIPTQTATVPTSTSAGSLGWVLREASNCAHEPSTQESSSPTKSPTSSKAPQNPFDHPTFARLRELYRLSKVLFDFICPQEYGIKSEEKLDIGLLTSLPLAKQILSDIQDMKKHPKSAAVVNYFTKESHIYTLLNVIYGSQLPMKIARNALPELDYLSQIAFELYEADDPTSLFGKKHSIRLLLSPGCHTQDPLDVQLDDDHYIGCIPRLSLTRHLDMDMVTQRLKSRFTRVSLPKTFTPVNISSPVVNPKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANS9</accession>
    <name>G3ANS9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_66688</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375290.1">
        <property type="nucleotide sequence ID" value="XM_007375228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANS9"/>
    <dbReference type="FunCoup" id="G3ANS9">
        <property type="interactions" value="858"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANS9"/>
    <dbReference type="GeneID" id="18875109"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66688"/>
    <dbReference type="eggNOG" id="KOG2588">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_582867_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANS9"/>
    <dbReference type="OMA" id="IKSAHNV"/>
    <dbReference type="OrthoDB" id="2133190at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052099">
        <property type="entry name" value="Regulatory_TF_Diverse"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47336">
        <property type="entry name" value="TRANSCRIPTION FACTOR HMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47336:SF2">
        <property type="entry name" value="TRANSCRIPTION FACTOR HMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="338"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="469" mass="52710" checksum="132CAB1EFAAC10C1" modified="2011-11-16" version="1">MSFKLNDYDAFLTSLFDKNNQKVKLEDADKLQVSPQYEQLEQANNQLHNSAMMQPPSQPSSYTTAQQPSQPPSRDEMMMRQHSLPSQHPGHMNEDGPFPVDFDFGLVAPGFAYTTTNTNRSKSLSFTIGNHGEPYAQPHHHPLQHQNNLDGSGNVTEFMAEMSAEHNNGPRSVENDNYNNFNDIENSRETPNNIFMQSMNHNNNSYTSDFGPIDEQSSSVMINHDSLSPISMGNSGSPAMHSDHSSNNILGMQHSEQLMRQATSITFNNIDDRVSPKMSKQFPATAFAPTLSSSSTTKKTKKQPRKAKSISGPISGHNSGGESDLSPTLNHRHPGRPRVKSAHNVIEQRYRNKINDKFNALQNSVPSLRILAQKKERERLQMKRSNTDKYDSSDDEPIDESLVTDENIDLEGLEPARKLNKATILANSIEYIKFLELKNNKMKADHEALLNKARMMGIIIDDDLDNDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANX7</accession>
    <name>G3ANX7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Long-chain-alcohol oxidase</fullName>
            <ecNumber evidence="16">1.1.3.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">SPAPADRAFT_67198</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Long-chain fatty alcohol oxidase involved in the omega-oxidation pathway of lipid degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>a long-chain primary fatty alcohol + O2 = a long-chain fatty aldehyde + H2O2</text>
            <dbReference type="Rhea" id="RHEA:22756"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17176"/>
            <dbReference type="ChEBI" id="CHEBI:77396"/>
            <dbReference type="EC" id="1.1.3.20"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a primary alcohol + O2 = an aldehyde + H2O2</text>
            <dbReference type="Rhea" id="RHEA:19829"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="EC" id="1.1.3.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Energy metabolism; methane degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 16">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.3.20" evidence="16"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375878.1">
        <property type="nucleotide sequence ID" value="XM_007375816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANX7"/>
    <dbReference type="STRING" id="619300.G3ANX7"/>
    <dbReference type="GeneID" id="18875149"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67198"/>
    <dbReference type="eggNOG" id="ENOG502QSD8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008878_3_1_1"/>
    <dbReference type="InParanoid" id="G3ANX7"/>
    <dbReference type="OMA" id="CFQGCKW"/>
    <dbReference type="OrthoDB" id="269227at2759"/>
    <dbReference type="UniPathway" id="UPA00147"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047639">
        <property type="term" value="F:alcohol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046577">
        <property type="term" value="F:long-chain-alcohol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046188">
        <property type="term" value="P:methane catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015945">
        <property type="term" value="P:methanol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012400">
        <property type="entry name" value="Long_Oxdase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46056">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46056:SF12">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028937">
        <property type="entry name" value="Lg_Ch_AO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0485">Methanol utilization</keyword>
    <keyword evidence="12 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="18">
        <location>
            <begin position="237"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase C-terminal" evidence="19">
        <location>
            <begin position="532"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR028937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR028937-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF05199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EGW32602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="699" mass="77673" checksum="2521C9ABCE630F34" modified="2011-11-16" version="1">MESVEDRHIDTFIALADGIIHETTLDNVRDSIAPDFPQEKLNEYVNSCTRPSKIPEFRDVVLDTLNKSNLEAVKDFVLVMEALSSMKLAKEFTGSNTLIHDMTLKEREKLLLSWRDSKVPLMRKLFRSVYSITMSGCVLLADDLHLQACGYPGKELRDEAYKNQIVDDFSYEFMERPTTEGAELYLPDIEAVIIGSGCGAGAVAHTLAESGFKSLILEKGKYFSTSQLNFSDLEGVSNLFQSGGPISTNDQELFILAGSNFGGGSTVNWSASIKTPFKVRKEWYDEFGLDFVATDTYDKCQEYVWNKMGTSTEGIHHSYANNMLLEGCEKLGYNAKVIDQNTGGHPQHRCGFCHLGCKFAIKQSTPNNWFREPAATGSKFMEQVKVLEIIHHNGYAQGVVCQDTVTGVKFKITGPRVYITSGGSLNTPVILQDSGFRNKNIGSNLKLHPATAIFGDFGNEVRTKAYADSIMTSVCTEVDDLDGNAHGAKIETILNAPLFQNAHLPWFGSDAARRHMLRYNNMTAMLLLARDTSSGSIRSDPERPDSYYIDYQVNKFDRQSLMKALVTTADILYIQGAKKIISPQPSVPVFESDKQKEERSIKDEDYVAWRNSVSTMSFYPYSTSFGSAHQMASCRMSGKGPEYGACDEKGRLFECGNVFVADASVMPTSSGVNPMITTYTLARYIGLNIAKELKIQAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANZ3</accession>
    <name>G3ANZ3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Heat shock protein SSA1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">HSP70</name>
        <name evidence="12" type="ORF">SPAPADRAFT_138204</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Heat shock protein that may play a role in the transport of polypeptides both across the mitochondrial membranes and into the endoplasmic reticulum.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Binds human histatin-5, an antifungal peptide from saliva.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375894.1">
        <property type="nucleotide sequence ID" value="XM_007375832.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANZ3"/>
    <dbReference type="FunCoup" id="G3ANZ3">
        <property type="interactions" value="1338"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANZ3"/>
    <dbReference type="GeneID" id="18870103"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138204"/>
    <dbReference type="eggNOG" id="KOG0101">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_3_0_1"/>
    <dbReference type="InParanoid" id="G3ANZ3"/>
    <dbReference type="OMA" id="SYAYNIK"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062040">
        <property type="term" value="C:fungal biofilm matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030446">
        <property type="term" value="C:hyphal cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10233">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000001">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000016">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="609"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="620"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00065230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="653" mass="70280" checksum="9337F8BABB259779" modified="2011-11-16" version="1">MSKAVGIDLGTTYSCVAHFTNDRVEIIANDQGNRTTPSFVAFTDTERLIGDAAKNQAAMNPANTVFDAKRLIGRKFDDPEVQTDKKHFPFKIINKGGKPAIEVEFKGETKVFTPEEISSMVLTKMKETAESYLGEKISDAVVTVPAYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDKKGSKEEHNVLIFDLGGGTFDVSLLAIDEGIFEVKATAGDTHLGGEDFDNRLVNFFAQEFKRKNKKDLTGNQRALRRLRTACERAKRTLSSSAQTSIEIDSLYEGIDFYTSITRARFEELNADLFRSTLDPVEKVLKDAKIDKSQVGELVLVGGSTRIPKIQKLVSDFFNGKELNKSINPDEAVAYGAAVQAAILTGDTSSKTQDLLLLDVAPLSLGIETAGGIMTKLIPRNATIPTKKSEIFSTYADNQPGVLIQVYEGERAKTRDNNLLGKFELSGIPPAPRGVPQIEVTFDIDANGILNVSALEKGTGKSQKITITNDKGRLSKEDIERMVSEAEKFKEDDEKEAARVQAKNGLESYAYSLKNTINDGEMKDKIPEADKEKLTKAIDDTISWLDASQAASTEEYGDKHKELESVANPIITAAYSAGGAPPPGGAGGFPGAGGAGGFPGGAPGGGAESSGPTVEEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3APD9</accession>
    <name>G3APD9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Coupling of ubiquitin conjugation to ER degradation protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_61200</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the CUE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375392.1">
        <property type="nucleotide sequence ID" value="XM_007375330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APD9"/>
    <dbReference type="FunCoup" id="G3APD9">
        <property type="interactions" value="106"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APD9"/>
    <dbReference type="GeneID" id="18873772"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61200"/>
    <dbReference type="eggNOG" id="ENOG502S35Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115919_0_0_1"/>
    <dbReference type="InParanoid" id="G3APD9"/>
    <dbReference type="OMA" id="TVNRFME"/>
    <dbReference type="OrthoDB" id="3824970at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14424">
        <property type="entry name" value="CUE_Cue1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000050">
        <property type="entry name" value="Related to AMFR protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00546">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="CUE" evidence="11">
        <location>
            <begin position="66"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="33"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="109"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="110"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="187" mass="20636" checksum="F633E10F6C06D9C6" modified="2011-11-16" version="1">MDYSTIIFIATLAIAFLFLRWLIAPIPQTNEFDIPPTASRSTAATTSANASATTTTSSQRRNRRPVTDSMIEVVQTIAPGLTREQIRYDLENTGSVELTVERYMELGDLPFPPGYTPSPAPPAEPETGSSSSNSTKAVSGKPVNLLEKYNVDVDSGYEGQTLLQRKQQMIANARKRLEKQLRNEIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3APG6</accession>
    <name>G3APG6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_51216</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375959.1">
        <property type="nucleotide sequence ID" value="XM_007375897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APG6"/>
    <dbReference type="GeneID" id="18871533"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51216"/>
    <dbReference type="HOGENOM" id="CLU_957010_0_0_1"/>
    <dbReference type="InParanoid" id="G3APG6"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013197968" description="Hyphally-regulated cell wall protein N-terminal domain-containing protein" evidence="6">
        <location>
            <begin position="16"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="7">
        <location>
            <begin position="7"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="291" mass="32694" checksum="F27F0B45DCBE0421" modified="2011-11-16" version="1">MWKIWIFLLLKIISALTINNEVITTSKSQASQSQDIIISPQGKYVFMQTPTITFSRNFENQGTFSVSTEQLQKLSLKFQYTSGKFLNKGTFTAITSDMAQATEFLIKVNQFTNEDNGYMKIESGSRSGTQNSLIEASSLKNKGIIIIKNQGVNRGTFQINLKTTSGIGGSWGSTKPAVEFSNAGTIELYFQNFKQGNWPIVGGGCIYLENKSLLELDTNNAANLQTFVFGEQSTIVINNSVKNFSFKFMNFAPGNIIQSKYLLRFSYRSDTGIVEYFHPERYHISNQNQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3APK2</accession>
    <name>G3APK2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">chitinase</fullName>
            <ecNumber evidence="3">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_55693</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="3"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375449.1">
        <property type="nucleotide sequence ID" value="XM_007375387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APK2"/>
    <dbReference type="STRING" id="619300.G3APK2"/>
    <dbReference type="GeneID" id="18871959"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55693"/>
    <dbReference type="eggNOG" id="KOG4701">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007818_7_2_1"/>
    <dbReference type="InParanoid" id="G3APK2"/>
    <dbReference type="OMA" id="PLDYLFI"/>
    <dbReference type="OrthoDB" id="6020543at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02877">
        <property type="entry name" value="GH18_hevamine_XipI_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045321">
        <property type="entry name" value="Cts1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025928">
        <property type="entry name" value="Flocculin_t3_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050542">
        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708:SF49">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13928">
        <property type="entry name" value="Flocculin_t3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0134">Cell wall</keyword>
    <keyword evidence="8" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="5" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442485" description="chitinase" evidence="16">
        <location>
            <begin position="20"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="17">
        <location>
            <begin position="21"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="311"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="312"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW32173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="552" mass="58145" checksum="CB43B333109D954C" modified="2011-11-16" version="1">MLPFKTLITTAVLTATALADSQIAVYWGQNGFGDQEPLATYCQNTNMDIVLLSFLNQFPDPLNVNFANQCGGTFTDSDLLHCSAIGEDIKTCQSLGKKVLLSLGGAVGKTGFANTTEAKDFADVLWNKFGGGDDDERPFDDAVVDGFDFDIEQGSTTGYPELATALKAKFAKDSSKSYYLSAAPQCPYPDTYVGDLISDVPLDYLFIQFYNNNCQVSGDFNFDVWQKFAESAPNPNIQLFVGVPGAPSDAITGFVTPKELAAALDDIKCDDNFAGVSLWDASGAFKDYNGDGAYIDQVRDVLDNLNVDECNETSSTEETSSADPTSTEAETTSADPTSTEAETTSADPTSTEAETTSEDPTSAPSSSAGFYGNSSTSAIQSVQTVSDIHTTVITITSCSENKCSATPVTTGYVIVTDVNTVYTTYCPLTNEDVTVTKAPSSAAEKCQEITKTVPVAALPTNKGVIAVGSTQAAQPSVEVSKQTETYVSGDITSTVTVAVTSTLESAVPTVGAYSAAGNGTIPIYSYEGAAVADSSRVALWTTIPLLMLAALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3APK8</accession>
    <name>G3APK8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PRMT5 oligomerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61265</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375455.1">
        <property type="nucleotide sequence ID" value="XM_007375393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APK8"/>
    <dbReference type="STRING" id="619300.G3APK8"/>
    <dbReference type="GeneID" id="18873806"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61265"/>
    <dbReference type="eggNOG" id="KOG0822">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010247_1_1_1"/>
    <dbReference type="InParanoid" id="G3APK8"/>
    <dbReference type="OrthoDB" id="1368803at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016274">
        <property type="term" value="F:protein-arginine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035248">
        <property type="entry name" value="PRMT5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10738">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10738:SF0">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17286">
        <property type="entry name" value="PRMT5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="PRMT5 oligomerisation" evidence="2">
        <location>
            <begin position="31"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="179" mass="21073" checksum="EE2386D520E2803D" modified="2011-11-16" version="1">MGSFGDNELCPEILAQFDKPRPDLIMIPSSYTSYIQPVYTPFKIKTISLIKLSNYYALGEEQQLWEWEHPGDFQPNRIAKLSFPNKKGYKVSGFLGWFTCTLYGHINIQIHSPSLSTYCKSWYPIYFPVHRTKVKPDHNIHLEFKRINNGENVWYEYTFHGKVYNENGVDYCMKLLDNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3APQ1</accession>
    <name>G3APQ1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription initiation factor TFIID subunit 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_61303</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF9 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375498.1">
        <property type="nucleotide sequence ID" value="XM_007375436.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APQ1"/>
    <dbReference type="FunCoup" id="G3APQ1">
        <property type="interactions" value="508"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APQ1"/>
    <dbReference type="GeneID" id="18873831"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61303"/>
    <dbReference type="eggNOG" id="KOG3334">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068315_3_0_1"/>
    <dbReference type="InParanoid" id="G3APQ1"/>
    <dbReference type="OMA" id="WNMKDEW"/>
    <dbReference type="OrthoDB" id="341924at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07979">
        <property type="entry name" value="HFD_TAF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000064">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003162">
        <property type="entry name" value="TFIID-31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051431">
        <property type="entry name" value="TFIID_subunit_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48068">
        <property type="entry name" value="TAF9 RNA POLYMERASE II, TATA BOX-BINDING PROTEIN (TBP)-ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48068:SF4">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN ASSOCIATED FACTOR 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02291">
        <property type="entry name" value="TFIID-31kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="19"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="23346" checksum="E1F4DEB4B39F29FC" modified="2011-11-16" version="1">MSVPASSGGAAQATINSSPATAIGTTTTTPAAAAAAVAPPSTAPAQAPATTQTDLNQPQPERKEAIVPRDVRLLHLIFATQGIQNYQDHVPLQLMDFAHRYTKSVLRDALVYNDHAKPINTNTNLNPNSNTTVNTDDIRLAIAARSNYQFKPTPPKELLLELAQERNSRPLPPVIPKWGLNLPPEKYCLTAKDWENLDEEPDLDDERIKKKAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3APU9</accession>
    <name>G3APU9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carboxymuconolactone decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61349</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375546.1">
        <property type="nucleotide sequence ID" value="XM_007375484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APU9"/>
    <dbReference type="FunCoup" id="G3APU9">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="GeneID" id="18873853"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61349"/>
    <dbReference type="eggNOG" id="ENOG502RCP9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065389_3_0_1"/>
    <dbReference type="InParanoid" id="G3APU9"/>
    <dbReference type="OMA" id="WGHLKGA"/>
    <dbReference type="OrthoDB" id="5537330at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052999">
        <property type="entry name" value="PTS1_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28180">
        <property type="entry name" value="CONSERVED MITOCHONDRIAL PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28180:SF2">
        <property type="entry name" value="PEROXISOMAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxymuconolactone decarboxylase-like" evidence="1">
        <location>
            <begin position="261"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="331" mass="37063" checksum="116A0C41CBAE84A4" modified="2011-11-16" version="1">MTILTAERLVRLAYNYPSLHNTWYLIATACLTIVNQPQEIPRLYHFALRQQLLDTPSDDSVLTDKHMLQLAQDSINSAKKYADLTGVGVNLPDLLVYTQHLPLKFKYSRSEDIHATQDRVTCRIREVILKSVALGGLPRAINALMILKTVTPTSLKAGLIPERNLIVHPGKIPSSSIVSEDFDGTSFEQQSTTDTIDGPISKQSIDVRQIKKELVRGSNFWNSVYTNKINTRIKQQMLTAYPDLWYFAYHHIYAPLLSYTDILSGKETSMCVVACLIPQDVNPQLKGHLKGALNNGATKQELDDVRSLTFDICDWAGGVHWQGGKEGVAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ62</accession>
    <name>G3AQ62_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 20">Autophagy-related protein 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">ATG9</name>
        <name evidence="22" type="ORF">SPAPADRAFT_51426</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Phospholipid scramblase involved in autophagy. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate)(out)</text>
            <dbReference type="Rhea" id="RHEA:67920"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:38663"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)</text>
            <dbReference type="Rhea" id="RHEA:38571"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out)</text>
            <dbReference type="Rhea" id="RHEA:38895"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 20">Preautophagosomal structure membrane</location>
            <topology evidence="3 20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 20">Belongs to the ATG9 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376187.1">
        <property type="nucleotide sequence ID" value="XM_007376125.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ62"/>
    <dbReference type="FunCoup" id="G3AQ62">
        <property type="interactions" value="251"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ62"/>
    <dbReference type="GeneID" id="18871554"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51426"/>
    <dbReference type="eggNOG" id="KOG2173">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006200_1_0_1"/>
    <dbReference type="InParanoid" id="G3AQ62"/>
    <dbReference type="OMA" id="ELMTISW"/>
    <dbReference type="OrthoDB" id="2020634at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061908">
        <property type="term" value="C:phagophore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007241">
        <property type="entry name" value="Autophagy-rel_prot_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038">
        <property type="entry name" value="APG9 AUTOPHAGY 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038:SF10">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04109">
        <property type="entry name" value="ATG9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 20" id="KW-0072">Autophagy</keyword>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13 20" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 20" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="501"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="590"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="701"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="68"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="764"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="156"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="766"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="785"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="796"/>
            <end position="807"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00024621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU364027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="EGW31409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="907" mass="106265" checksum="BDF78D173CFF1444" modified="2011-11-16" version="1">MASYNPYRDDIASDKDGIDYESNQSFQQHQLGMGSHNDTFLSRIFGLNSIYNQLQENYQYYDPEFDSTYQQTQQLQQSISNDFQQQEQPEDEDESLLPSKTAVAPLINTHEEGRNSTGPGHSNSLLDSESDSDLSSSSESYVRPKSNRVRVEQQQEEQQQQQQQQQKVDMEEQGNSHKLKFNLPNPKRLITGRYNRQDGPILPTHTTPSQFRKPKPAENGQKQNQRRATFVIPPKERALYLWANITNMDEFLSDVYYYYCGKGLANIIISRIIDLLILVFILLFTVFLKWGINYEYFAQWRQEQDKHITLSDLIIPNFLFNRVPILAKFLLFGFFIYVLLRVIQLYFDYNYKLREIRNFYHYLINIDDEELMTINWKTIVERLMLLKDYNTLTSTNDPQRHYVNDLSSKVRLNAHDIANRIMRRENYMIAMMNKDILDLTLFDNRFFSITQNSILTKTLEWNLKLCIDNFIYNQNGQINSSILKDYNRNQLAKELTSRFKLAAIINLLLCPFIAIYFVLLYFYRYFNEFKSNPSSLVGSRQYTPFAQWKLREFNELPHFFNKRLLLSIGPANTYINQFPKGYIAINVMKLINFVSGALLAILVIMGIWYEDESHSFWAFELTEGRSSLFYISIFGTIWAVTGNTPTSASEISSNSESFVYDPEASLRYVSQFTHYLPSTWNKKLHTIEVKNSFCELYSLKILIILNEIFSIVLTPFILWFKISANSGAIIDFFREYSIHVDGLGYVCYFAMFNFEEKDKNMMVNLNKRKPKKPKNKGKPSGNEIEMNKISKSDHEETSDDTSDDDSDLNYMRQHYQQDDKMIKSYMYFLESYGGSKSNPKSTPQIQAKRQVPVEPSPSFLYDQQSISDSLYNINYKFDDSTILQDDISRRKGGVLGMLNQFYKQDIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQF3</accession>
    <name>G3AQF3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_154739</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376277.1">
        <property type="nucleotide sequence ID" value="XM_007376215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQF3"/>
    <dbReference type="STRING" id="619300.G3AQF3"/>
    <dbReference type="GeneID" id="18870998"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_154739"/>
    <dbReference type="eggNOG" id="ENOG502SA4F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067614_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQF3"/>
    <dbReference type="OMA" id="NKCKNLH"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019496">
        <property type="entry name" value="NUFIP1_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10453">
        <property type="entry name" value="NUFIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="109"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="109"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="77"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="82"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="212" mass="24600" checksum="347C9415BB4F930D" modified="2011-11-16" version="1">MFDLPTHLPNELPTHLPNQPTHTEPVQEPTEDILDEEQQAKPNPNEPIYIPGTNITLQTEEDIAKWIEERKKKWPSKQVVAKKLEQKQSEIQQKEDRKRPAESKEAPGKRTKAVCRFYQQNKKCKFGNKCKNLHVIEANPLAGHELTHYKRTVNGIPVLIPKLYSNRTHNTPSKSSLFKHLISRDQSVENDQIIEFIKFLDKQGLIDHNVHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQG7</accession>
    <name>G3AQG7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Thiamine phosphate synthase/TenI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_140906</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:47848"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62890"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:47844"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>4-methyl-5-(2-phosphooxyethyl)-thiazole + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + H(+) = thiamine phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22328"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:58296"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-(2-hydroxyethyl)-4-methylthiazole + ATP = 4-methyl-5-(2-phosphooxyethyl)-thiazole + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24212"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17957"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58296"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">In the C-terminal section; belongs to the Thz kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">In the N-terminal section; belongs to the thiamine-phosphate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376292.1">
        <property type="nucleotide sequence ID" value="XM_007376230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQG7"/>
    <dbReference type="FunCoup" id="G3AQG7">
        <property type="interactions" value="124"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQG7"/>
    <dbReference type="GeneID" id="18870331"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140906"/>
    <dbReference type="eggNOG" id="ENOG502QS2M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019943_1_1_1"/>
    <dbReference type="InParanoid" id="G3AQG7"/>
    <dbReference type="OMA" id="GQTDMPI"/>
    <dbReference type="OrthoDB" id="4994at2759"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00139"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004417">
        <property type="term" value="F:hydroxyethylthiazole kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004789">
        <property type="term" value="F:thiamine-phosphate diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01170">
        <property type="entry name" value="THZ_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00564">
        <property type="entry name" value="TMP_TenI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000104">
        <property type="entry name" value="Thiamine biosynthetic bifunctional enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00228">
        <property type="entry name" value="Thz_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00097">
        <property type="entry name" value="TMP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000417">
        <property type="entry name" value="Hyethyz_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036206">
        <property type="entry name" value="ThiamineP_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022998">
        <property type="entry name" value="ThiamineP_synth_TenI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034291">
        <property type="entry name" value="TMP_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006830">
        <property type="entry name" value="PRK09355.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00693">
        <property type="entry name" value="thiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20857:SF23">
        <property type="entry name" value="THIAMINE BIOSYNTHETIC BIFUNCTIONAL ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20857">
        <property type="entry name" value="THIAMINE-PHOSPHATE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02110">
        <property type="entry name" value="HK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02581">
        <property type="entry name" value="TMP-TENI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01099">
        <property type="entry name" value="HYETHTZKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51391">
        <property type="entry name" value="Thiamin phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamine phosphate synthase/TenI" evidence="18">
        <location>
            <begin position="7"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00061146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW31514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="500" mass="53480" checksum="F388C37812D123A5" modified="2011-11-16" version="1">MTVDYSLYLVTDSTMIPESSTFLRQVELAIANGATIVQLREKKLSTLDFITRAQQVHALTKAKGIPLIINDRVDVALAIDAEGVHVGQDDMPAELVRRLIGENKILGVTCSVPEEVEAVTASGIADYVGLGTVYATNTKKDVTDPEGTGPIGIRKMLQVLGRYNQSHEKKINSVAIGGINHTNADKVMYECAVDGERIDGVAVVSCIMASENAGKATSDLLKIIETTPNWASRSCIQEADIGKFVAQTHPLVHHITNNVVKNFSANVTLALGASPIMSELAAEFHEFASDIPMIALVLNLGTPTPELMSVFKTAIQVYNKYAKHIVFDPVACGATQARLQASKKLLNSGHVSVIKGNLGEIQGIWKLTSTYSESSNSGSLMRGVDSIAKLTEEKIIQIGKEVSKDFKTVVVITGKVNYIIDGDKYTKSEGGSELMGLVTGSGCSLGSTIAAFLATGADVYLSAVDAVNLYNEAGRRAAGYSSTPGSFMISFVDELYKLTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQH9</accession>
    <name>G3AQH9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Inactive metallocarboxypeptidase ECM14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Inactive metallocarboxypeptidase ecm14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_62113</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Inactive carboxypeptidase that may play a role in cell wall organization and biogenesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376304.1">
        <property type="nucleotide sequence ID" value="XM_007376242.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQH9"/>
    <dbReference type="FunCoup" id="G3AQH9">
        <property type="interactions" value="849"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQH9"/>
    <dbReference type="GeneID" id="18874188"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62113"/>
    <dbReference type="eggNOG" id="KOG2650">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019326_1_0_1"/>
    <dbReference type="InParanoid" id="G3AQH9"/>
    <dbReference type="OMA" id="PPNHKDL"/>
    <dbReference type="OrthoDB" id="3626597at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03860">
        <property type="entry name" value="M14_CP_A-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000060">
        <property type="entry name" value="Putative metallocarboxypeptidase ecm14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF147">
        <property type="entry name" value="INACTIVE METALLOCARBOXYPEPTIDASE ECM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442561" description="Inactive metallocarboxypeptidase ECM14" evidence="13">
        <location>
            <begin position="20"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="14">
        <location>
            <begin position="180"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW31526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="492" mass="57734" checksum="E52B0A968E08680A" modified="2011-11-16" version="1">MQHFQKIGLILTLLGLSTALQWQFPFDISKFRLNGGDSTHLHFSTVDPSDFPIDLLKYHDDYVIRVNYGDNAELKRFLLSKSGDSSNDTQIKFSRWTRNTSTRQIDLQIDENNLIKLIEKFPLISYDIIIEDLSQKVYETYPKDFHNSITTKDSKYQYQSTHDVINATTANVFSEVFFKEYRPLETIDSWLELLQETYPDILSIEEIGHTYENRPYKVVHFAVPNDDVKHGDRRTIVITGGTHAREWISVSSVLYAIYDLLQFYAEEPESKIFKELDFLFIPVANPDGYEYTWKADRLWRKNRQQTVQPNCFGIDLDHSYDYHWTKSTDWECGEEYSGEYPFEAIESKIWEEYLNNTNHDHKIWGYIDLHSYSQEVLYPYAYSCDQQPRDEENLIEAAYGIAKSIRLQSGVNYHVLPACIDRDADLLPDLGSGSALDFMYHNRAYWAFQLKLRDSGSHGFLLPPKYIEPVGREVFAGFKYFCYFILSDERQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQP0</accession>
    <name>G3AQP0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TECPR1-like DysF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_154964</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376365.1">
        <property type="nucleotide sequence ID" value="XM_007376303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQP0"/>
    <dbReference type="FunCoup" id="G3AQP0">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQP0"/>
    <dbReference type="GeneID" id="18871004"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_154964"/>
    <dbReference type="eggNOG" id="ENOG502QUW8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034954_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQP0"/>
    <dbReference type="OMA" id="WRLIFIQ"/>
    <dbReference type="OrthoDB" id="74314at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052816">
        <property type="entry name" value="Peroxisomal_Membrane_PEX28-32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010482">
        <property type="entry name" value="TECPR1-like_DysF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28304:SF1">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28304">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06398">
        <property type="entry name" value="Pex24p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="309"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="TECPR1-like DysF" evidence="6">
        <location>
            <begin position="118"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="521" mass="60546" checksum="B44274CE02BE8487" modified="2011-11-16" version="1">MDKPSYRERALGVLSQAIEKSNTIAQSNPGTKRAIAAGTASILLEMGLESYDKQEEPILTSDQISQIESIAESAQEQPKSKHFADRLIEKLIQTSLPENVPDRDKYEARLKESKQPGMSIAILTSNMRKLLVKMSIFFHVYYGGIHVLTWKRPAKTLSVLVLYTAVCIWPHLVLVYPLIFVLFGVFIPGYVHRHPMRRPDLIKVKRRGQSLWNYFFDTDEISVLEEFMDEQEYFNESPATPDSDELSDEMSVVTEITTPDDETIETRKSQLDIVMNLRDLQGLTTDLLVGIEKGEQFYSDIGGFKDERLSTFIFYGTVLATGIVMFLGQFIPWRLIFIQSGWAVMLLLHPKAKKLLVNAQKLQKPKDEVKTEVEPAVKEDKPFDRNDIIVDDSPEVRMVEVFELQMKSILKSEWKFYRYTNTLYDKSNKSRVAGKRPRGVDDLSKVLPPHDWKFDFGLVNKWKVDEEPVKFLTERSYDIKLFKLKNDEWIYDDMENVEQDIVYEFRRRRLSRDCFRYGRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQU9</accession>
    <name>G3AQU9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Arginase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_56452</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the arginase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376424.1">
        <property type="nucleotide sequence ID" value="XM_007376362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQU9"/>
    <dbReference type="STRING" id="619300.G3AQU9"/>
    <dbReference type="GeneID" id="18872033"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56452"/>
    <dbReference type="eggNOG" id="KOG2964">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039478_1_0_1"/>
    <dbReference type="InParanoid" id="G3AQU9"/>
    <dbReference type="OMA" id="RNDIRCG"/>
    <dbReference type="OrthoDB" id="288726at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11592">
        <property type="entry name" value="Agmatinase_PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.10:FF:000001">
        <property type="entry name" value="Agmatinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF28">
        <property type="entry name" value="HYPOTHETICAL ARGINASE FAMILY PROTEIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0464">Manganese</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0745">Spermidine biosynthesis</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR036979-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="359" mass="39292" checksum="30ADFFC24A53A792" modified="2011-11-16" version="1">MRSQLNLPDKDDTSDIQSDQNMYGGILTYAHFPHFNCFNPEFYDDKEIDIAIVGAPFDTGVSYRPGARFGPEAIRSSARRLGEIAVDKKHKGKKYPLDPYDSTTHDFKIIDCGDVAMTPFDNRIALNQLYRGIRAIHKHNPGSASRAHPKTITMGGDHTITLMALKSISERLGKPVKVIHFDSHIDTWDPTVLGGGVTDYMKLNHGTFLHYAHELGYITNKGNFHVGIRAPLIDSLYDSEHDSECGFSIIRASDIDKVGVQGIVKTLKEALGTEDPVYISVDIDVLDPSTAPGTGTMEIGGFTGRELLSILNGLKGINLVGADVVEVSPPYDTNSEITSLAATSVIDSFLQLMVIQVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQZ7</accession>
    <name>G3AQZ7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_72420</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376434.1">
        <property type="nucleotide sequence ID" value="XM_007376372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQZ7"/>
    <dbReference type="FunCoup" id="G3AQZ7">
        <property type="interactions" value="529"/>
    </dbReference>
    <dbReference type="GeneID" id="18875434"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72420"/>
    <dbReference type="eggNOG" id="ENOG502QRPQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005934_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQZ7"/>
    <dbReference type="OMA" id="SCLTRCA"/>
    <dbReference type="OrthoDB" id="4159781at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF12">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="45"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="951"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1017"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="78"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="954"/>
            <end position="980"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1082" mass="121510" checksum="1C32F8E0C1EFC663" modified="2011-11-16" version="1">MSAPQLYTFDTGNSITKQADSQPASAAATTSTTVAVRKRKRPSLVCSICKKKKIKCDKQLPCNNCIKSKCSDACTYDRSKSPNKLEDNRHSDVPSLPYVSSAKNPIILADHRAKKPKVVSPEKSSDEQVTVSLSELNMLKERLQQIESSINVAPPTYKKAPTPGVLPPPIMSSAWNSASPQPQDGSQGQVFATSYNISHTRSQSPPSNGIQLPPLNFNPPPNGISTNGISTNGISPNGISPNYAGSAKSEDSLIGYNPCLNSEDTINFYENYTSIYVKGPHRRVNFGPFAWSSMMRRDRALSTLWDYVAKRKEKDSSQTLVFAEVTNEVNQENNEVATNESNESEATFRKRALEADGYGDLIPYTNILKRRRPGIQETSELNRCTLPLGLTFYEGQVEKELQLIDKIPLILPKKKVAWKLIDRFFTYLYPFMPFVDEMDFRQKIAAIIGSESLEDTPIKNLKVEKRLDLAIIGTFLLIMRMSYLSLFSNKTDVNEERLTTTDPSPEAQDVKYLLQNPISITTAIVANCCLSQFEILQKTSFPVLQLALYLRIYHTYAPEDGGGPDGGESQTLTAVIIQMAYSLGLHRDPDNFNDVLNDKRQNHLMRKIWHHLVLSDIHNCYTFGIPPAIDPDSYDTKIPFHEEGNENLKDKTLDRFTTACYFPSYTDLAGILRSLLKLVLNVRGKIKLEELCKLISEFEVLMTHKYGTLEDCLNPPTKDTHIFARNFPTKFYISLKAFCVSIFYHIFIHYEPIDLNLSFFYMKKIIKIACTDLMPHYFELLGNSDVICDMMINPKLQQIVHSLNQVNLAIIVRVNVTVYYLRSHADHDARCAADPSYYTYYKELCKLSSCLTRCAEFSIAAVSKLSSRYYHAWRITKGHTYLLRTVTTLDFYKDNLKNNHKDTPTNGLPGYSTEQIAELVTMCETALDRVGKNCVMGTEFCTEVNYKQFNREPSSNSTDTTSSKNTDTTATTASTLATDSPSVNNTKSYTNEFGLNLENSSEIDNVWIQMLTFKQERDGESAAPTPGKFDFNDKMSKSPNSAARLKNIGSGAGMGLSVDQDLSLDMFGDLAFGSMFDGDYAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR34</accession>
    <name>G3AR34_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">20S-pre-rRNA D-site endonuclease NOB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_62301</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for the synthesis of 40S ribosome subunits. Has a role in processing 20S pre-rRNA into the mature 18S rRNA, where it is required for cleavage at the 3' end of the mature 18S rRNA (D-site). Accompanies the 20S pre-rRNA from the nucleus to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
            <location evidence="7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the NOB1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376473.1">
        <property type="nucleotide sequence ID" value="XM_007376411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR34"/>
    <dbReference type="FunCoup" id="G3AR34">
        <property type="interactions" value="1074"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR34"/>
    <dbReference type="GeneID" id="18874260"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62301"/>
    <dbReference type="eggNOG" id="KOG2463">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024666_2_1_1"/>
    <dbReference type="InParanoid" id="G3AR34"/>
    <dbReference type="OMA" id="GYELECE"/>
    <dbReference type="OrthoDB" id="446759at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09876">
        <property type="entry name" value="PIN_Nob1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000020">
        <property type="entry name" value="20S-pre-rRNA D-site endonuclease NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.10">
        <property type="entry name" value="Nin one binding (NOB1), Zn-ribbon-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039907">
        <property type="entry name" value="NOB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017117">
        <property type="entry name" value="Nob1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036283">
        <property type="entry name" value="NOB1_Zf-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014881">
        <property type="entry name" value="NOB1_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033411">
        <property type="entry name" value="Ribonuclease_PIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814:SF2">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17146">
        <property type="entry name" value="PIN_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08772">
        <property type="entry name" value="Zn_ribbon_NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037125">
        <property type="entry name" value="D-site_20S_pre-rRNA_nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144206">
        <property type="entry name" value="NOB1 zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PIN" evidence="10">
        <location>
            <begin position="8"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="119"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="373"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="453"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="121"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="156"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="375"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR037125-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW31695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="476" mass="54445" checksum="3EA9A3D1BF8A379B" modified="2011-11-16" version="1">MSDTAKIQAVVLDAGPLITQTASALQQYASEYYTTPGVHAELKDEHARQQLILWGDKLNVRHPKPEFIQKVISFAKLTGDYSVLSVNDLHIIALAYELEVERNNGDWRLRKFPGEQLQLKKQQEEKKEEDATKTKEDEEDGFTVVEHKPKQGNSNRPRRRGGRRQREKREAELRKQMEQGNSEEFESVTDDTITQVEETTVPEETETQATTESTEDVNIEAHDETSETYELTEEYNEEDDDGEWITPDNLQEELLKDNNEETQEASATGPLIKVALATGDFACQNVAMQININLLNAMSGKQIKRVRNYMYRCHACFRLTPINKNGQPKHFCPKCGGNTLLRCAVSVDTLTGKVTPHLKANFQWYKRGERFTLPSPLSKNSQKKQGNAGYQHNKENRHKSLQSPLILREDQKEYQQALKNDEWQRRQNEKMLQEWIGGGSADNYVSPFSTNMNNIRPSGVRVGRGRFVNSSKGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR45</accession>
    <name>G3AR45_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Pre-mRNA-splicing factor PRP46</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 7">Pre-mRNA-processing protein 46</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_140502</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in pre-mRNA splicing and required for cell cycle progression at G2/M.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the WD repeat PRL1/PRL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375998.1">
        <property type="nucleotide sequence ID" value="XM_007375936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR45"/>
    <dbReference type="FunCoup" id="G3AR45">
        <property type="interactions" value="983"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR45"/>
    <dbReference type="GeneID" id="18870296"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140502"/>
    <dbReference type="eggNOG" id="KOG0285">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_72_0_1"/>
    <dbReference type="InParanoid" id="G3AR45"/>
    <dbReference type="OMA" id="FAMCFDQ"/>
    <dbReference type="OrthoDB" id="10256122at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045241">
        <property type="entry name" value="Prp46/PLRG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19923:SF0">
        <property type="entry name" value="PLEIOTROPIC REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19923">
        <property type="entry name" value="WD40 REPEAT PROTEINPRL1/PRL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="1 6 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="66"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="109"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="156"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="198"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="240"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="362" mass="40490" checksum="218484F93BD77D58" modified="2011-11-16" version="1">MTSELIPSDELAEAMYNNVKLDYLLANTDNIPPEPAQNNTITSQSAEPISSPQPTSVQWKPLRTLANAHNGWIRSIAIDHSNKFFVTGSSDTTIKIRTVANPHTLLATLTGHIMAVRTFSISSRFPYLFSGSEDKTLRCWDLERTNAKEGCLIRQYHGHVGGIYATSLHPELDLLISGGKDQVVRVWDIRTRTEAMTLVGHRSDVTSIETLPFDPQIITSSMDGTVRLWDLRKQSTQVTLTHHTKSIRSMKLHPEEMTFISGDSNGIINQWLLPKGELLNQFDPTENESKIINTLSVNPVTNTVFAGYDDGKTGFYNYITGKLEHIGYFPNPSTVYASAFDQSGLRLITCHGNKSIRVWHCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR49</accession>
    <name>G3AR49_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF292-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61800</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IST1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376002.1">
        <property type="nucleotide sequence ID" value="XM_007375940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR49"/>
    <dbReference type="FunCoup" id="G3AR49">
        <property type="interactions" value="790"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR49"/>
    <dbReference type="GeneID" id="18874055"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61800"/>
    <dbReference type="eggNOG" id="KOG2027">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037652_2_0_1"/>
    <dbReference type="InParanoid" id="G3AR49"/>
    <dbReference type="OMA" id="HEFVEMS"/>
    <dbReference type="OrthoDB" id="29853at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042030">
        <property type="term" value="F:ATPase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099638">
        <property type="term" value="P:endosome to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.60:FF:000002">
        <property type="entry name" value="Vacuolar protein sorting-associated protein IST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.60">
        <property type="entry name" value="Vacuolar protein sorting-associated protein Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005061">
        <property type="entry name" value="Ist1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042277">
        <property type="entry name" value="IST1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161">
        <property type="entry name" value="IST1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161:SF5">
        <property type="entry name" value="IST1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03398">
        <property type="entry name" value="Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="182"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="190"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="27059" checksum="EE00AE6005804E2D" modified="2011-11-16" version="1">MPTPPPLNPLRLKTSLKMSLSKLKFLQEKKTALCKQQRRQLADLLRQGKESSATIRVENIIRDDIYIELLEYLELYCELLLARINLITDPAKHECDKSLLEAVSSVIYAANHTELKEVVSIKDWLIAKYGHEFGRNALENKDEVVPEKIVSRCSVEPPQETLVDLYLCEIARTYSAPYSKLSEYSAQSSEGEDNDDDDDNDGEGGTKELEAPLAADAKPKVKEQDEFDALKARFAALRTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARN7</accession>
    <name>G3ARN7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cullin family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_141567</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376568.1">
        <property type="nucleotide sequence ID" value="XM_007376506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARN7"/>
    <dbReference type="FunCoup" id="G3ARN7">
        <property type="interactions" value="643"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARN7"/>
    <dbReference type="GeneID" id="18870383"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141567"/>
    <dbReference type="eggNOG" id="KOG2165">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007149_4_0_1"/>
    <dbReference type="InParanoid" id="G3ARN7"/>
    <dbReference type="OMA" id="ERYYEFP"/>
    <dbReference type="OrthoDB" id="5581181at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007091">
        <property type="term" value="P:metaphase/anaphase transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044554">
        <property type="entry name" value="ANAPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057975">
        <property type="entry name" value="TPR_ANAPC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45957">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45957:SF1">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25773">
        <property type="entry name" value="TPR_ANAPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin family profile" evidence="2">
        <location>
            <begin position="412"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="614" mass="70459" checksum="B548DF2786BD4B81" modified="2011-11-16" version="1">MNIEITSKVISQIIPRPGLYDLGGDLDNDIQIVLDWLSPYYPENLNNNKPTKIVDPSVRVKKAIHGYLKHESNQIEFVMVYINTISSQFETKFVADLSFLEYIHMVRQLSHYYNCHLDYLNLTNRSRMIFTRKLNLLINSKLSHTKMEEYFAERLFGDLSSQSHFELIDVITTLTSINLTNELNSTMIQLAISTIKRNIKEIATEKWTVPILDSINNCITTQIYPTFSLVTSYTESPAASYLSSLNTTAHDELVHLRISEIYTMVSTYPSSIPALEELHLCLTSSTKRRQLTTSFIDSMRKHLLHSGADTVDIIHIYIKTMQSFLIIDPRGVLLDQVIRPARKYLRTRDDVVIKLVHGMLNNNPSSKLYELAVELTRKQESIVRDEGLKWTPDPIDALADFKRGKHDVIESLLSIFTSKEVFIDEFTRLFGKQLIEGGDVQDIEEKLVLLKSRFGNDLFTMLDIMIRDIRQSGLYQGDLLRMSVLSHLYWPSSMLEHDSFSVPDLIQQRFDEFKSQFINDKPGRTVKHIPSLGSVTLELEINHETKSFVVTPLQAAIIDLFNEKSDEVSVNDAATKLSISEYTATQALNFWVDEKVLMKLTSTLYIANEDDESF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>G3ART1</accession>
    <name>G3ART1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_61916</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ART1"/>
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    <dbReference type="InParanoid" id="G3ART1"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="54" mass="6028" checksum="D8E0E5A922BD272E" modified="2011-11-16" version="1">MAAVANYLEICECNLRSITLLCVYSSTSITFMGLMKRGNLFANILVEHINKFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS24</accession>
    <name>G3AS24_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62483</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376651.1">
        <property type="nucleotide sequence ID" value="XM_007376589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS24"/>
    <dbReference type="STRING" id="619300.G3AS24"/>
    <dbReference type="GeneID" id="18874340"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62483"/>
    <dbReference type="eggNOG" id="ENOG502S6CT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_608320_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS24"/>
    <dbReference type="OMA" id="FWSGKNE"/>
    <dbReference type="OrthoDB" id="30195at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="501" mass="55847" checksum="0ED766D9C6627EB5" modified="2011-11-16" version="1">MRFAVQEGGKYIACSISRHGKNEIRFYKLSQENETVTSSVANSIFLENSIIDIIWSTSVSKSPKRRKRTSEDTPVDISNDTLIALDESGNLIVLSPLSNKPIKQFTTRVPGTKLIKADNFIWIKSKDSLIKYSSDDDTVISIQVPSATTIEVLPGKAPHVVLSDGSKLSLGKIVKNKFVQEHELEIKGVTQIIQSKTKPDLLAVLADELYIVNLKDLDHYTKIEYTGNATVLSTVSYIAEEYIQVSTQEKVYLVRFGDKQVSKTITTNNVTELFTHESILVGAYKDLNDVEISDIHWISDNEGEIINTETTKSSSSSLPNAKIHIPKITAINNVEYSQLLETLLEKLNSTELNKNSIVKLCSSINNEETIKQTIKALVFDQVSNTSIEKLYSIISNEVSKDCSINKSLGIWLKWILLTYGGVIAKSDQSNLKNLQKELSSGLELLPHLIVIQGRLQLLTLQSEIRQKVIDIEESTEEKDIVYANGEGDDDLEPEVVDIDVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS81</accession>
    <name>G3AS81_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">U3 small nucleolar RNA-associated protein 13 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_142668</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377073.1">
        <property type="nucleotide sequence ID" value="XM_007377011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS81"/>
    <dbReference type="FunCoup" id="G3AS81">
        <property type="interactions" value="1101"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AS81"/>
    <dbReference type="GeneID" id="18870468"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142668"/>
    <dbReference type="eggNOG" id="KOG0319">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009276_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS81"/>
    <dbReference type="OMA" id="PYVQRHF"/>
    <dbReference type="OrthoDB" id="5414888at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013934">
        <property type="entry name" value="Utp13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854:SF15">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08625">
        <property type="entry name" value="Utp13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="112"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="138"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="184"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="244"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="371"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="417"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="471"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="513"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="555"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="598"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 13 C-terminal" evidence="7">
        <location>
            <begin position="651"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="630"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="796" mass="89818" checksum="05B91FD13C648785" modified="2011-11-16" version="1">MASLKTTYKQHNNIEPFYVGGISCTISSDGQFLATPLNEDVIITNLYTNEIIHKIEGDGELITNVVITPDGKNLAILSQSQQLRIFDVTLGTVTKTFKLPSPVYISTADFTSSLFAFGGSDGVITVWDIQNGYVTHSLKGHGTTICSLNFYGELNSTDWKLASGDTMGTVKIWDLVKRKCKYTIKEHNTAVRGLGFDDEGEYFISAGRDNVAIIYNTKNFRPLFTHPINEQIESAGFVRLQHNNRQYFYTAGSENILRIWDIKTGELVAKSTTPLKTSEELLIIDVIKLDDTSLYLVVSDQTIIEVSLQEETITSKEDHLEFPIVKRIAGNHGIIADIRYVGPEFNLVALATNSPALRVIDPSKPLELKLYEGHTDLLNAIDVSDDGKWIATASKDNEARLWRWNDEEEEFESFARFQGHAGAVTAISISKSGGNIEVPKFILTGSSDLTIKKWKIPSAPNSTVKVSEYTRRAHDKDINSIDISPNDEFFATASYDKLAKVWNTDSGETIGILKGHKRGLWDINFYKFDKLIATASGDKTVKVWSLVDFSCKKTFEGHTNSIQRVKFFNSDSPQLLSAGADGLIKLWDYKSGEVIKSLDNHDNRIWALDVKEDGQDFISADADGKLTEWVDNTEEELLLKEQQNKERIEQEQNLSNFIKNKDWANAFLLALTLDHSMRLYNVIKASIETNEDPESPIGSKQLEETIVELNDDQLIKLFKKVRDWNINFKFFELSQKLLAVILNNIAMEKLVDIPGLMKIMDSIVPYNERHYSRIDDMIEQTYILDYAVEQMNKLIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASD0</accession>
    <name>G3ASD0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyrroline-5-carboxylate reductase</fullName>
            <ecNumber evidence="5">1.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_141869</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-proline + NADP(+) = (S)-1-pyrroline-5-carboxylate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="5"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376703.1">
        <property type="nucleotide sequence ID" value="XM_007376641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASD0"/>
    <dbReference type="FunCoup" id="G3ASD0">
        <property type="interactions" value="463"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASD0"/>
    <dbReference type="GeneID" id="18870406"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141869"/>
    <dbReference type="eggNOG" id="KOG3124">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042344_1_2_1"/>
    <dbReference type="InParanoid" id="G3ASD0"/>
    <dbReference type="OMA" id="YYFIESL"/>
    <dbReference type="OrthoDB" id="10263291at2759"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3730.10:FF:000001">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.10">
        <property type="entry name" value="ProC C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053790">
        <property type="entry name" value="P5CR-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00112">
        <property type="entry name" value="proC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645:SF0">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00521">
        <property type="entry name" value="P5CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 4 5" id="KW-0521">NADP</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="6">
        <location>
            <begin position="4"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase dimerisation" evidence="7">
        <location>
            <begin position="166"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="8"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000193-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="273" mass="28813" checksum="E8B1B1E84F6C1A7B" modified="2011-11-16" version="1">MPYTITVLGCGVMGTAVTSAILKAQFDPYPTKIIACTVPSEEANLKKVFADNKLVSVSAGVENNNKAVAEADVIILGCKPYMYEGIYNEVKDSLTGKQLLISLLAGTTIKELSIFTPYVAKVMTNTPARFGCGTAAIAFAPEVTEEQQELVMKLIDTVGMTVKIPEKNMDIATSLIGSGPAFCLLMMESMIDGAVRMGMPYDIAQKSAAKVMEGTAKMVLETGEHPAALKAKVCTPGGTTIGGLLKMEDKGVRSAIARGVEEAAIISASFAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASE0</accession>
    <name>G3ASE0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Type 2A phosphatase activator TIP41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_142108</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIP41 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377091.1">
        <property type="nucleotide sequence ID" value="XM_007377029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASE0"/>
    <dbReference type="FunCoup" id="G3ASE0">
        <property type="interactions" value="823"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASE0"/>
    <dbReference type="GeneID" id="18870423"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142108"/>
    <dbReference type="eggNOG" id="KOG3224">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039187_0_2_1"/>
    <dbReference type="InParanoid" id="G3ASE0"/>
    <dbReference type="OMA" id="DMILFED"/>
    <dbReference type="OrthoDB" id="10253878at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051330">
        <property type="entry name" value="Phosphatase_reg/MetRdx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007303">
        <property type="entry name" value="TIP41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021">
        <property type="entry name" value="GAF/PUTATIVE CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021:SF16">
        <property type="entry name" value="TIP41-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04176">
        <property type="entry name" value="TIP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="47"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="383" mass="43825" checksum="7B4D0E907D33DB1B" modified="2011-11-16" version="1">MSQDNQEQSVSSKESTSKSKTVFTMPGNRPGIQAVHVNAAREMVRLHTQGRRPPSSTTASTAVKGPVPPLPSTASSFPGASPHPHVSIPEGCKNPQCTHCGKVIIPAPQSSYPIQDTPAIAVKNWKIYTIKKPILNSTELEDLEARFHFPLPEMIFGNSRVKIVNEVSGESIEFNALDALDSLDSESHLKVSYHKEWLDSRRSEREKKEKEERGEVESRDLREFTDKLDTIEPYDWTYSPNYKGTTNIKFSESSQEIPIHRLTQPDPILFFDEAVLFEDELADNGISILNYKIRVMPTCLLLLCRFYLRIDNVIFRIRDTRVFVDFDQDLVIRDYRQQEYEYSELLKSVQRKSIGNDPKKLFRDHDWVSKNIPTINKVVESNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT71</accession>
    <name>G3AT71_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Glutamate synthase [NADH]</fullName>
            <ecNumber evidence="19">1.4.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">GLT1</name>
        <name evidence="28" type="ORF">SPAPADRAFT_141734</name>
    </gene>
    <organism evidence="29">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="29">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Forms L-glutamate from L-glutamine and 2-oxoglutarate. Represents an alternative pathway to L-glutamate dehydrogenase for the biosynthesis of L-glutamate. Participates with glutamine synthetase in ammonia assimilation processes. The enzyme is specific for NADH, L-glutamine and 2-oxoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>2 L-glutamate + NAD(+) = L-glutamine + 2-oxoglutarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="1.4.1.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="24">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="24">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-glutamate biosynthesis via GLT pathway; L-glutamate from 2-oxoglutarate and L-glutamine (NAD(+) route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Energy metabolism; nitrogen metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Nitrogen metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.14" evidence="19"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376867.1">
        <property type="nucleotide sequence ID" value="XM_007376805.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AT71">
        <property type="interactions" value="663"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AT71"/>
    <dbReference type="GeneID" id="18870395"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141734"/>
    <dbReference type="eggNOG" id="KOG0399">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_8_1_1"/>
    <dbReference type="InParanoid" id="G3AT71"/>
    <dbReference type="OMA" id="WDGPAAM"/>
    <dbReference type="OrthoDB" id="4327079at2759"/>
    <dbReference type="UniPathway" id="UPA00045"/>
    <dbReference type="UniPathway" id="UPA00634">
        <property type="reaction ID" value="UER00690"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097268">
        <property type="term" value="C:cytoophidium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016040">
        <property type="term" value="F:glutamate synthase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016639">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019676">
        <property type="term" value="P:ammonia assimilation cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097054">
        <property type="term" value="P:L-glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00982">
        <property type="entry name" value="gltB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00713">
        <property type="entry name" value="GltS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02808">
        <property type="entry name" value="GltS_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000160">
        <property type="entry name" value="Glutamate synthase (NADPH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1060.10:FF:000006">
        <property type="entry name" value="Glutamate synthase (NADPH/NADH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000031">
        <property type="entry name" value="Glutamate synthase 1 [NADH]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000017">
        <property type="entry name" value="Glutamate synthase [NADH], amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000113">
        <property type="entry name" value="Glutamate synthase [NADH], amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000022">
        <property type="entry name" value="Glutamate synthase [NADH], amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.60:FF:000001">
        <property type="entry name" value="Glutamate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000001">
        <property type="entry name" value="Glutamate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="entry name" value="Glutamate synthase, alpha subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028261">
        <property type="entry name" value="DPD_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006982">
        <property type="entry name" value="Glu_synth_centr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012220">
        <property type="entry name" value="Glu_synth_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002932">
        <property type="entry name" value="Glu_synthdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006005">
        <property type="entry name" value="Glut_synth_ssu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051394">
        <property type="entry name" value="Glutamate_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01317">
        <property type="entry name" value="GOGAT_sm_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008730">
        <property type="entry name" value="PRK11750.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43100">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43100:SF1">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14691">
        <property type="entry name" value="Fer4_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00310">
        <property type="entry name" value="GATase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04898">
        <property type="entry name" value="Glu_syn_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01645">
        <property type="entry name" value="Glu_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000187">
        <property type="entry name" value="GOGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="Alpha subunit of glutamate synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18 24" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="25" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0274">FAD</keyword>
    <keyword evidence="9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0288">FMN</keyword>
    <keyword evidence="17" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="13" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="15" id="KW-0408">Iron</keyword>
    <keyword evidence="16 24" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 28" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="27">
        <location>
            <begin position="50"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1573"/>
            <end position="1600"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="1544"/>
            <end position="1572"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="23">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1180"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1186"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1191"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00057049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00068518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR000187-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR000187-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="EMBL" id="EGW30834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="2129" mass="235034" checksum="043AF813F7BD686E" modified="2011-11-16" version="1">MSNTSSYLPREEFSTTNIYNYDESPANKSWACALPVAQGLYNPELEKDACGVGFSCHLKGIPSHKIVSDARNLLCNMTHRGGELNPKDGDGAGLLTSIPHKFFKREFKYYCNVDLPAPGQYGTGNLFFKRDEAVFEKSKATFESIAESLGLKVLGWREVPHDSTILGPASLSREPFILQPAIVLQELLGQGSIDEAEFKSKYSRQFEKKLFILRKQSSHTIGLHNWFYICSLSNKTIVYKGQLAPNQVYNYYHDLANSEFEAHFALVHSRFSTNTFPSWDRAQPLRLAAHNGEINTLRGNKNWMRSKEGVMASQLFGDEMDKLFPIIEEGGSDSAAFDNVLELLVINGIVSLPEAVMMMIPEAWQNDQNIDPKKKAFYEWAACLMEPWDGPALFTFADDRYCGANLDRNGLRPCRYYVTDDDFIICASEVGVIEIEPERVLQKGRLQPGRMLLVDTVEGRIVDDRELKSKVASRYDFKSWIMANMISLQDLNSKLENRGINITADDIDTSIKLDSDPRLIASGYSHEQLSFVLAPMAEGNEALGSMGNDNALACLTDQPKLLYEYFRQLFAQVTNPPIDPIREAIVMSLECYVGPQGNLLEMKPDQCNRLLLKSPILDNSELLAIQNIEHVYPKWSVANIDITFDKQAGIPGYISTIDRICQEASKAIADDNKIIILSDVKTGPSRVPISALIAVGAVHHHLVRQKQRSKVALIIQTQEAKEVHHACCLVGYGADAINPYLAMETLVRMNDQGLLKIKKSPQEIIKGYKSAIDAGILKVMSKMGISTLASYKGAQIFEALGIDNSVIDRCFAGTASRIKGVTFEYIAQDAFTLHERGYPTRETTKPQGLPETGEYHWRDGGERHINDPAAIASLQDAVRNKNERAYETYAKKENEAVRNCTLRGLLDFDYESGNSVPIDQVEPWTEIVRRFFTGAMSYGSISMEAHSTIAVAMNRLGGKSNTGEGGEDAARSQVNANGDTMRSAIKQVASGRFGVTSYYLADADELQIKMAQGAKPGEGGELPGHKVSAEIGKTRHSTPGVGLISPPPHHDIYSIEDLKQLLYDLKCANPRARTSVKLVSEVGVGIVAAGVAKAGSENILVSGGDGGTGAAKLTSIKYAGLPWELGLAESHQTLVLNDLRGRVILQTDGQIRTGRDIAIACLLGAEEWGFATTPLIAMGCIYMRKCHLNTCPVGIATQDPILRKKFPGTPEHVINFFYYLAQDLRQIMAKLGFRTINEMVGRAEKLKVRDELRNIKNANIDLSPILTPAHTIRPGVATYCVRKQDLKLHVRMDNKLIDESEMTLAKGLPVTIDCDIVNTDRTLGTTLSYRVSKTFGEQGLPHDTIHVNVTGSAGQSFGAFLASGITLELEGDANDYIGKGLSGGRIIVYPPKDSKFKAEDQIIAGNTAFFGATSGTAFIRGIAAERFAVRNSGANIVVEGTGDHGCEYMSGGRVIVLGGTGRNFGAGMCGGIAYVLDMGQDFGDRVNSKTVELSPVTETEEIAFIRSLIEDHRHYTGSEVADNILNDFTRFLPRFVKVLPHDYKKVLEKEKLKAEEAKKQELNKFLKSIKEDPTADATNGEASKIKLGRGHTPLTSKAGGAEPKVLDVEDTIFNTDFEKKAPVKLDKLRGFMKYKRRNERYRDAKDRTKDWNEMTSRLTKEELKYETARCMDCGVPFCTSDTGCPISNVIPKWNELVFQDRWFDALQRLLMTNNFPEFTGRICPAPCNGACVLGISDDPVNIKSVECAIIDHGFEQGWIKPQPPAQRTGKTVAIIGSGPAGLACADQLNKAGHTVTVYERSDRPGGLLMYGIPNMKLDKGIVKRRTDLLEAEGVEFVCNTTVGEDITVSELKSKHDAVVFAVGSTIPRDLRIPGRSLKNIDYAMTLLHSNTKALLDNNLETIRKTIEGKKVIVIGGGDTGNDCLGTSTRHGAKSVTNFELLPNPPNERPKDNPWPQWPRIFRVDYGHTEVKTHYGKDPREYSILSKEFIDDGEGNVKGIKTVRVEWKRSDSGAWQMAEVPGSDEIFEADIVLLSMGFLGPEADNLEVEKTKRGTITTVDANGYQVAKDDNVFAAGDCRRGQSLVVWGIQEGRQCAREVDLYLEGATRLPGNGSVEQRDYKLLEELAEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATL7</accession>
    <name>G3ATL7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin interacting protein 3 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_52169</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377013.1">
        <property type="nucleotide sequence ID" value="XM_007376951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATL7"/>
    <dbReference type="FunCoup" id="G3ATL7">
        <property type="interactions" value="63"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATL7"/>
    <dbReference type="GeneID" id="18871635"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52169"/>
    <dbReference type="eggNOG" id="ENOG502QS95">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005287_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATL7"/>
    <dbReference type="OMA" id="RFSAYHM"/>
    <dbReference type="OrthoDB" id="783096at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005519">
        <property type="term" value="F:cytoskeletal regulatory protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1540">
        <property type="entry name" value="Actin interacting protein 3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022782">
        <property type="entry name" value="AIP3-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005613">
        <property type="entry name" value="AIP3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056279">
        <property type="entry name" value="Aip3p_Bud6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051825">
        <property type="entry name" value="SRCIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22741:SF10">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN CG32809"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22741">
        <property type="entry name" value="P140CAP/SNIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03915">
        <property type="entry name" value="AIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23153">
        <property type="entry name" value="Aip3p_Bud6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00806">
        <property type="entry name" value="AIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Actin interacting protein 3 C-terminal" evidence="4">
        <location>
            <begin position="247"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="119"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="649"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="449"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="153"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="164"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="224"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="649"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="668" mass="75006" checksum="669E11426B9561A1" modified="2011-11-16" version="1">MTGSRRLNSIETSVTRLLVSTKHLLESLTQWARQEADDKFVSDAYVKLGNDFRAATRAFTSNGIDISDIGNVPQALRIILEAALSEAPSQENLDRFLPNIRNIIVTLLQNLKAKQAKAKSLSQDRSSKSSIESAASPATTVSPPSENLLKPSSVEHDFPERVASHSSQPPVKNDALSQLQKGDALQRRASKRFSAYQYAKLANISTPSTLPSITAARSSLANESSHEDIPERKPINATNDGNKSYVFLKINKKTKKVDIKFPVTFASLRLLFVEKFAYSPGSASFPEIYIQDPGSGVLYELEENLLSDVKSGTLLCLNEPDSQAKAINDLESKIQDLSKKIEAMNANVISQVKESISSIDIPAPVLPTSHKEVNTATGTSSYDLKKIRKIEQDLKALRQLHRSSVDNFKSIISSLTEKLDQFQEYGLEISKDSNRSFMETCHTKLSEESDVLITKVDDLQDLVEELRKDVAQRGVRISEKQIKNTVKEIENAKSALRSMTDFISKERNVWKKIWETELDKVCEEQHFFKVQDELTIDLEEDIKKMEETFTLIEQCSIEQSKGSTRKRNKVVANLYIPEPGESLHDIKDAVLNEVVALVPDHQSRVEAIERAEKLREKEREMMKLNQFQEELGDFVEDKKLKKSGGIAEVEKMRSQKDSENLKSSFGIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATP3</accession>
    <name>G3ATP3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63117</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377240.1">
        <property type="nucleotide sequence ID" value="XM_007377178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATP3"/>
    <dbReference type="STRING" id="619300.G3ATP3"/>
    <dbReference type="GeneID" id="18874602"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63117"/>
    <dbReference type="eggNOG" id="KOG2588">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_607160_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATP3"/>
    <dbReference type="OrthoDB" id="2133190at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052099">
        <property type="entry name" value="Regulatory_TF_Diverse"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47336">
        <property type="entry name" value="TRANSCRIPTION FACTOR HMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47336:SF2">
        <property type="entry name" value="TRANSCRIPTION FACTOR HMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442612" evidence="1">
        <location>
            <begin position="21"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="451" mass="51455" checksum="D8D67E0FA6E0ADBF" modified="2011-11-16" version="1">MTKLLLVLGSVASIIIPIFNQVRTPAKKNNNKNSSSHPLIDWFLISFGMKLPNKLSPEERTSIIGHLQGSAKGSWSTLVSDYFYLNTCEINFENCFLSLLIGRMLQSKYTKFTPIINHQLMMKAGLLMNLHDKSDDKYLKKLNQLVGKVDGISMFASEDLIARLTNVFEGKLIHNGITDGQNHIKYVELYQEFQNDYYGIVLNWRLLEIIHQLNLTYLEELNEDHEQILNDVQTIEKILDQKSSIYKYFELFVTIIDSKRSPNLYASLRGKVQNSLDRFKAEIEGLDLTDHEIYNTSDEENTELEDAPPAPPKVKPSLRAQRSLVASLGLVDEEEFIILTSCLILYFTSLQQPSQASRLLFYLKDSSSKLTLLGFTALVTLINNLKPTADEDSEALDILVKNCREWLNDTKLTDFMEVELRSELTKIVVNKGMILNGVEDETEDNVVINKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATS0</accession>
    <name>G3ATS0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Nuclear envelope protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_52393</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NDC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377267.1">
        <property type="nucleotide sequence ID" value="XM_007377205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATS0"/>
    <dbReference type="FunCoup" id="G3ATS0">
        <property type="interactions" value="145"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATS0"/>
    <dbReference type="GeneID" id="18871674"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52393"/>
    <dbReference type="eggNOG" id="ENOG502RZSR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028040_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATS0"/>
    <dbReference type="OMA" id="WQTANLF"/>
    <dbReference type="OrthoDB" id="67850at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070762">
        <property type="term" value="C:nuclear pore transmembrane ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106166">
        <property type="term" value="F:spindle pole body-nuclear membrane anchor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070631">
        <property type="term" value="P:spindle pole body localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019049">
        <property type="entry name" value="Nucleoporin_prot_Ndc1/Nup"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13269">
        <property type="entry name" value="NUCLEOPORIN NDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13269:SF6">
        <property type="entry name" value="NUCLEOPORIN NDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09531">
        <property type="entry name" value="Ndc1_Nup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="10" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="14" id="KW-0261">Viral envelope protein</keyword>
    <keyword evidence="14" id="KW-0946">Virion</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW30296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="606" mass="70062" checksum="B05DEE0A54DCA47C" modified="2011-11-16" version="1">MDNTSKMNNYQYIFTKVMNKRLKFILNINIIMAVIISITLRLPCGQFWWNLIWTFAIRGPALFTALSLIRQARKKFSTVDYSVHKSLGSQIYHSVFSMTFVKYSLFYVISSYLIHAVYMFQLPFAFDYYLLSKEYRQNPLINDEWVYYWYCPLVVALLYSSHHIIFQRNRLQLEYGNSKRTPEKYLFSRIPQLIGHSLGLSIIIAIVTPLSYYFFKPLIYKTNVLLILLLGLDPSIPPSATSFKTYLHLAYVAYTTYLGWEIANHTFEVYATIGCLDGNKPISTYSADPINCLLSGLRNVDPQYSLSRVTAFQELAYISMAKDPEGIKLRNAIYNARNRKGYLWPAIFDECSLVIKEMTDRVNYRSISDLKALKEGKPDGTAFEFSKDDNIFGNSFISSVRSSPVTNIKEYKKEEPTKSKYDFIYTILAKNKIPLPKITNHKLITALRQKYQIGCDYYNKFSTTLLNTWIGVFFRTTLKRDTESRVINPVNFGNAVIAVANLLIHSIEEDKNNTITANDVCQVLNLFERPIRACSNYIDYVPASVYVNDDSKNNLVGIVHDLTMHEFYELCVKFNGKLNDLVLTPKTYKLAKWVIDVAIAEQQKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATX2</accession>
    <name>G3ATX2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">40S ribosomal protein S13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_63204</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377319.1">
        <property type="nucleotide sequence ID" value="XM_007377257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATX2"/>
    <dbReference type="FunCoup" id="G3ATX2">
        <property type="interactions" value="979"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATX2"/>
    <dbReference type="GeneID" id="18874639"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63204"/>
    <dbReference type="eggNOG" id="KOG0400">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090139_1_0_1"/>
    <dbReference type="InParanoid" id="G3ATX2"/>
    <dbReference type="OMA" id="MHTRRKG"/>
    <dbReference type="OrthoDB" id="623277at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.10:FF:000003">
        <property type="entry name" value="40S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.130:FF:000001">
        <property type="entry name" value="40S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_A">
        <property type="entry name" value="Ribosomal_uS15_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_uS15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023029">
        <property type="entry name" value="Ribosomal_uS15_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012606">
        <property type="entry name" value="Ribosomal_uS15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006331">
        <property type="entry name" value="PRK08561.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885">
        <property type="entry name" value="RIBOSOMAL PROTEIN S15P/S13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885:SF6">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08069">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01386">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS15 N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="151" mass="17034" checksum="86822C5B9C08ABE4" modified="2011-11-16" version="1">MGRMHSSGKGISSSALPYSRNAPSWFKLTSDEIVEQVIKYARKGLTPSQIGVILRDAHGVSQSKVVTGNKILRILKSNGLAPEIPEDLYYLIKKAVSVRKHLERNRKDKDSKFRLILIESRIHRLARYYRTVSVLPPNWKYESATASALVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU56</accession>
    <name>G3AU56_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peroxin 20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63273</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377403.1">
        <property type="nucleotide sequence ID" value="XM_007377341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU56"/>
    <dbReference type="STRING" id="619300.G3AU56"/>
    <dbReference type="GeneID" id="18874669"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63273"/>
    <dbReference type="eggNOG" id="ENOG502S3XI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_665674_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU56"/>
    <dbReference type="OMA" id="DHHWDEM"/>
    <dbReference type="OrthoDB" id="5407351at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.230">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="411" mass="46480" checksum="36F05AC2D37A5B30" modified="2011-11-16" version="1">MDSASCGPTSALQNLSKHAQRDTSLQHSQSRNIQTGANPIHGFRSGNIDPRLNNDFQRFNQSGDIGGQAFMNNVRLQTESPQIQGQPQMMGQPGWVNDFSNLSINKSTQQHPIQSNDWQQQFMQIRQQQQAQPQAQVAQGSQITPGYMSGRLNYGMNSMVQSHPVYEQQSEHQEVHKMEQQQKQFESQFDMIEQELMAQARTAETDAVEIEIADSNNAKDKEEFAQTARKVQSAMLSANSQDEEMQNKINNSSFLKLMNSIGNRHVELEGDKLVDVASGQDIREQQQNQEDIASAAPRTLDNHCIVDNPVHHDGWVQDGQINLQQQRAQSSDVKDPAHENKLPDPLAHIKDGQLEDINDPFTAARIISGGQVQANDWMDDVDWLDQTVPAPHINRMMQESYDDYRHEDDLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU82</accession>
    <name>G3AU82_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosome subunit biogenesis protein NIP7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_143743</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with pre-ribosome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NIP7 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377429.1">
        <property type="nucleotide sequence ID" value="XM_007377367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU82"/>
    <dbReference type="FunCoup" id="G3AU82">
        <property type="interactions" value="1124"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU82"/>
    <dbReference type="GeneID" id="18870568"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143743"/>
    <dbReference type="eggNOG" id="KOG3492">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097217_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU82"/>
    <dbReference type="OMA" id="LISMGTC"/>
    <dbReference type="OrthoDB" id="27490at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd21146">
        <property type="entry name" value="Nip7_N_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21151">
        <property type="entry name" value="PUA_Nip7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.10:FF:000002">
        <property type="entry name" value="60S ribosome subunit biogenesis protein NIP7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.220:FF:000001">
        <property type="entry name" value="60S ribosome subunit biogenesis protein NIP7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040598">
        <property type="entry name" value="NIP7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055359">
        <property type="entry name" value="Nip7_N_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016686">
        <property type="entry name" value="Ribosomal_synth_fac_NIP7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005155">
        <property type="entry name" value="UPF0113_PUA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17833">
        <property type="entry name" value="pre-PUA_NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03657">
        <property type="entry name" value="UPF0113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017190">
        <property type="entry name" value="Rbsml_synth_fac_NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88802">
        <property type="entry name" value="Pre-PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PUA" evidence="8">
        <location>
            <begin position="95"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR017190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW30458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="181" mass="20459" checksum="DA09884E4F3CEE6D" modified="2011-11-16" version="1">MRPLTEEETKVVFEKLANYIGRNISFLIDNPTNPHVFRLQKDRVYYVSDQIAKFATSVSRNNLMSLGTCFGKFTKTGKFRLHITALPYLAQYAKFKVWIKTNGEMPFLYGNHVLKAHVGRMSDDIPEHAGLIVYSMNDVPLGFGVSAKSTNEARSLAPTGIVAFRQGDIGEYLRDEDTLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU83</accession>
    <name>G3AU83_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">tRNA pseudouridine synthase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">tRNA pseudouridylate synthase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">tRNA-uridine isomerase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_157524</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Formation of pseudouridine at positions 27 and 28 in the anticodon stem and loop of transfer RNAs; at positions 34 and 36 of intron-containing precursor tRNA(Ile) and at position 35 in the intron-containing tRNA(Tyr). Catalyzes pseudouridylation at position 44 in U2 snRNA. Also catalyzes pseudouridylation of mRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
            <dbReference type="Rhea" id="RHEA:56644"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a uridine in tRNA = a pseudouridine in tRNA</text>
            <dbReference type="Rhea" id="RHEA:54572"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13934"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>uridine in snRNA = pseudouridine in snRNA</text>
            <dbReference type="Rhea" id="RHEA:51124"/>
            <dbReference type="Rhea" id="RHEA-COMP:12891"/>
            <dbReference type="Rhea" id="RHEA-COMP:12892"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377431.1">
        <property type="nucleotide sequence ID" value="XM_007377369.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU83"/>
    <dbReference type="FunCoup" id="G3AU83">
        <property type="interactions" value="1024"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU83"/>
    <dbReference type="GeneID" id="18871084"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157524"/>
    <dbReference type="eggNOG" id="KOG2553">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021971_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU83"/>
    <dbReference type="OMA" id="NKAFDCR"/>
    <dbReference type="OrthoDB" id="10256309at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02568">
        <property type="entry name" value="PseudoU_synth_PUS1_PUS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.580:FF:000002">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.660:FF:000002">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041708">
        <property type="entry name" value="PUS1/PUS2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF4">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="18">
        <location>
            <begin position="326"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="15">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00079072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00080858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR641708-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR641708-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW30460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="525" mass="60639" checksum="B99B34961EB90F76" modified="2011-11-16" version="1">MSEAENQQKSETIHYDDQVDDSVYKRDQKSKWTRTRTGDRIESEAKRTRKQLGVERRKKEYEDGNNYEFPLDEHGNPIKSERKPKRKCAVMIGYCGTGYNGMQLQNDPTIKTIERDLYDAFGKAGAISRENAEDMKKSGFMRAARTDKGVHAAGNVVSLKLIIEDPDIVKKINEHLPDQIRVWGIQRTTKGFDCRKLCSSRIYEYLLPTYSLLPPKKNTVLSDLLEEKRAKCPDLFHDDPEGAKWWEETHQKILDAGITQEQLDSIGTTIESTGPESEDATDKPVVFEDGELTEFGKLVKQVKAIENQSRRSFKISTERLNQFREAMKQYEGTHNFHNFTIGKHFKDPSASRFMKTTTVSDPFVIEGTEWVSIKIHGQSFMLHQIRKMIAMATMVVRIGCPVNIIQDFFGNKKINIPKAPALGLLLENPVYEAYNQTLQKLDYDPIDFTKFDEEMEAFKMKYIYDKIYAEEHKENVFYGFFGYIDTFKSNSDEERPDGTSVFDFIKNYTNKSTEQQVPSKEDPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUE2</accession>
    <name>G3AUE2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">TBP-associated factor 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_157654</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377489.1">
        <property type="nucleotide sequence ID" value="XM_007377427.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AUE2">
        <property type="interactions" value="654"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUE2"/>
    <dbReference type="GeneID" id="18871092"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157654"/>
    <dbReference type="eggNOG" id="KOG1932">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002317_2_0_1"/>
    <dbReference type="InParanoid" id="G3AUE2"/>
    <dbReference type="OMA" id="EQPDYQW"/>
    <dbReference type="OrthoDB" id="308861at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09839">
        <property type="entry name" value="M1_like_TAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000011">
        <property type="entry name" value="Transcription initiation factor TFIID subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
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        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
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    </dbReference>
    <dbReference type="InterPro" id="IPR037813">
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    </dbReference>
    <dbReference type="InterPro" id="IPR057991">
        <property type="entry name" value="TPR_TAF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25316">
        <property type="entry name" value="TAF2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25577">
        <property type="entry name" value="TPR_TAF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 Ig-like" evidence="10">
        <location>
            <begin position="635"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 TPR repeats" evidence="11">
        <location>
            <begin position="805"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="292"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1406"/>
            <end position="1507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="377"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1434"/>
            <end position="1449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1460"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1473"/>
            <end position="1487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW30518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1507" mass="170405" checksum="425D4CC1EE2897A1" modified="2011-11-16" version="1">MATTTTATTGATPRSRIRSKLSNLSSQLLKVGHQRINIDIDLANNSIDGFTELTIIPTSNMLKTVKLDCREMEIRDIFVNGIRCVNYIHKDMLYINDPAYFEGCLEDGRVNLLDLYSDRFGMHQHHMIRQKLSYLFRDTHTSADATTTTTSVPTSDYTTNTEELVIILPETIKFELTDINTVTPQSAITPSYLKSKPTSSEVYTPIQIKIDYRLQNPKNGINFVTNSNIDRRNWHAYTVNSTYGVSTSSWVPCIDNLWERCTWSLEVNIPRTIKDIGNPRIIGSKEAIQYAQSRRNTAVDDDDDQEDDEQQDDDENLDLTVCSGDFNNVKETPHPIDLSKKVVSWSLFNPVCAHHVGWAVGCFSSFVLANQESTANESEEQDFEEEQDSEFKPGSTPITVYALPQDIELARNTTEVTSSALDYFIKQFGSFPFSSYSLVFVQFQPSSADGYAGLSILSNNLLYPTNIIEPMFSTTQTLLNSIAHQWSGINIVPQTVHDSWCTIGISHFMASTYLRKLMGINEYKYRIRQQMRDIIKQDIGKRPLANQYYRFPIVETDLDFVKLKAPIVLFILDKRMTKTDKSFGLSRVLPKLFLQAMSGDLPNGTITTTHFQYLCEKVNRNKLDGFFKQWVYSTGTPHLRIQQKFNKKRGMIEMQIRQVQHLEKRQARLTPRSFITDATSYIEDEPGHPLLPVFTGPITIRVHEADGTPYEHIVELKEGNVKIDIQYNSKMRKPKKKDIEAGLDMMSPFTSRLGDVLTSPADMSSWGLSEFEKRDEEMMFNDPFEWIRVDVDYEWIAVIDIKQPDYMFAAQLCHDRDIEAQLESVRYFRGLDKPSVVHCTVLVRTLMDDRYFYGVRIAAAEALAKFSNSSNNFIGLGYLLRAYKELFCFRGSTIPKSNDFADFRQYFIQVAIPKILSKVRDQDGIVPPVIQELLLNLVKFNDNTNNSFTDGIYVSKLIEALTTTAVTGRKDEPHTKQFAKHVNTEIDRLLKLDQYMPSYHNIIEITCIKQKIRLAVANIIDLSFEDLLYFTLDKYPMEARVEAFTGLFRLGGLRNSAILNYFLKVCLLNWDRPMYRQSLISSLIESIAVAAIEGTQSLLDDVEFTTSQAVQASSKTGMIIIEDGSEMEKQRDSYARATVRGAIELLRRDYSIGNGLRGCLWELIHSSLVSVSERRDLFSVCSVLYREVDKMLVKLKVPSMDVTEFNRKIVAKNLGGGKVVFKREGRFKIQLATRKSISSKLKLSLKPAAPSGMAAAAAAAAVSTPIASAHTPVAKASTVAPPVPKVTIPIVAPAKKSLVKVENTRVTFKLPKVKLSRFRDVLVKHNGSVVTLKFSQRNRHRLESLRAEPVHRYVKITKFKTVEISSKPFESKPIRVSKPVDTLPVEVSKPAPSLKLTIRPLKEPRVSAVVTEPKESRAGAMQPKESANNSPEKVVPKESPKESAEKANEAKVSPKPASESPKESKETPKSRSKSSSPEPTNSNSPFSRSVSPYTKKKKTKIYIHTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUU8</accession>
    <name>G3AUU8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 NT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_158395</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377806.1">
        <property type="nucleotide sequence ID" value="XM_007377744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUU8"/>
    <dbReference type="FunCoup" id="G3AUU8">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="GeneID" id="18871124"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_158395"/>
    <dbReference type="eggNOG" id="ENOG502SBEF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033548_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUU8"/>
    <dbReference type="OMA" id="SSGYCYV"/>
    <dbReference type="OrthoDB" id="3365224at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039931">
        <property type="entry name" value="EEIG1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21456:SF1">
        <property type="entry name" value="C2 NT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21456">
        <property type="entry name" value="FAMILY WITH SEQUENCE SIMILARITY 102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 NT-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="209"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="265"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="394" mass="44318" checksum="28BB3B2A468CBD25" modified="2011-11-16" version="1">MFTSNKPKFAFSLSINELTNIPEINGACYIKLQIKDGHHRNKLTDLIKPVPPSASTAGTNSSSSSVNKSFFNNSSVNCTTSKKKIHNFKCVFNYKVSCNLRFGLKKKENLISNKYLLLKVYYVHESTKRSDGGSSSQTTELGRLDINLAEYLNFDEPITTKYLLNQSKVNSILNLTIQLNELPESFDFHTQLQIDDNSSANSSKKLPKPTHTSSSTTSFPKRTTFNVPQFENKHVFGGINTVFGEGTFQNSTPNHPPTPTDEKSSVPTPSVTASATTAATTTNTTTTRGINSKPQILIDPIVGTLYCKILESNWDPELYKLLEYSPDQCINDIFENNNNPYGHNQKLKQYVDEHINYQDDDEDEDSKPRELNGLISETKYREDLKSWKLTRIPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUW6</accession>
    <name>G3AUW6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RWD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_63673</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377823.1">
        <property type="nucleotide sequence ID" value="XM_007377761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUW6"/>
    <dbReference type="FunCoup" id="G3AUW6">
        <property type="interactions" value="777"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUW6"/>
    <dbReference type="GeneID" id="18874861"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63673"/>
    <dbReference type="eggNOG" id="KOG4018">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084528_2_0_1"/>
    <dbReference type="InParanoid" id="G3AUW6"/>
    <dbReference type="OMA" id="QWDEHKK"/>
    <dbReference type="OrthoDB" id="277175at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031333">
        <property type="term" value="P:negative regulation of protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903833">
        <property type="term" value="P:positive regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23823">
        <property type="entry name" value="RWD_GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040213">
        <property type="entry name" value="GIR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12292">
        <property type="entry name" value="RWD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="2">
        <location>
            <begin position="9"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="191" mass="22538" checksum="99F32988A42E9622" modified="2011-11-16" version="1" fragment="single">MDPAEEQQQELEVLESIYPDELTVISPTHFIIRVQLDTPSQRKHYLDLIVRYPPTYPEVIPNLDLEIPEISEEEEDSDDDDEDEDDDDTKAIKLALNMAEVIEFTRDELALLLSKLNEEAELNIGMPSVFALTTQLKDEAEALFVQILETRQKEYEREREEREREEQKKFIGTKVTKESYLEWRDKFRAEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUY7</accession>
    <name>G3AUY7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_144524</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377611.1">
        <property type="nucleotide sequence ID" value="XM_007377549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUY7"/>
    <dbReference type="FunCoup" id="G3AUY7">
        <property type="interactions" value="248"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUY7"/>
    <dbReference type="GeneID" id="18870635"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144524"/>
    <dbReference type="eggNOG" id="KOG4697">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081382_1_0_1"/>
    <dbReference type="InParanoid" id="G3AUY7"/>
    <dbReference type="OMA" id="EYEMVGM"/>
    <dbReference type="OrthoDB" id="10259843at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019185">
        <property type="entry name" value="Integral_membrane_SYS1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12952:SF0">
        <property type="entry name" value="PROTEIN SYS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12952">
        <property type="entry name" value="SYS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09801">
        <property type="entry name" value="SYS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW29845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="191" mass="22117" checksum="F5F1CA248027695B" modified="2011-11-16" version="1">MILSSGYSKLFNGYQPNDINNPSKLLVQIVILQCFYYLSALVMFGLFAWLNGYDFKIDWIFSWELVASDNAMGLTLFILWLFDSLLCVLFVTIIVGRSKLAWDFAITVHIINLIVVWSYSGKFPTSILWWCLQVLSGVILVTLSTYSTRWKELRTTFFENMLDQQELGQVQHTNNEEFEMTTFPKDSSSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV86</accession>
    <name>G3AV86_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_63509</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377655.1">
        <property type="nucleotide sequence ID" value="XM_007377593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV86"/>
    <dbReference type="STRING" id="619300.G3AV86"/>
    <dbReference type="GeneID" id="18874788"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63509"/>
    <dbReference type="HOGENOM" id="CLU_954949_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV86"/>
    <dbReference type="OrthoDB" id="4085918at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW29889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="292" mass="32427" checksum="6B825255BF593465" modified="2011-11-16" version="1" fragment="single">MLRVVQVITGVVVVGLSVASAVLLLLSPRSSSPHQISHEPVPLLNVVSGLLLSVLIIINSIIIGYIQLSLQLVISLVYIVSGILQVVTTPSTSHTIIFIVDVIFITSSMLISSINYLQPLSLLPPSSPQQQHHHHQQDEESTIGSTIHQDEKYTYKLSTPNNPIRTSISTPNFVTHQTKKNNNKVQTSNSLSTLVSASTNNISRDPSSLLMLEVDDEEEQEEDVTDIVRRSKSMIVSRKVKRWKSIHEEKAFLSNVNDCLLPPVLKGNKEKSPIQEQQEIQDLPYIAEFDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVG2</accession>
    <name>G3AVG2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sorting nexin MVP1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Sorting nexin mvp1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_158723</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377946.1">
        <property type="nucleotide sequence ID" value="XM_007377884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVG2"/>
    <dbReference type="FunCoup" id="G3AVG2">
        <property type="interactions" value="161"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVG2"/>
    <dbReference type="GeneID" id="18871132"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_158723"/>
    <dbReference type="eggNOG" id="KOG2273">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_399000_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVG2"/>
    <dbReference type="OMA" id="WEYAGAK"/>
    <dbReference type="OrthoDB" id="10064318at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07597">
        <property type="entry name" value="BAR_SNX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06866">
        <property type="entry name" value="PX_SNX8_Mvp1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000042">
        <property type="entry name" value="Sorting nexin mvp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028662">
        <property type="entry name" value="SNX8/Mvp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035704">
        <property type="entry name" value="SNX8/Mvp1_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045734">
        <property type="entry name" value="Snx8_BAR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47554">
        <property type="entry name" value="SORTING NEXIN MVP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47554:SF1">
        <property type="entry name" value="SORTING NEXIN MVP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19566">
        <property type="entry name" value="Snx8_BAR_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="11">
        <location>
            <begin position="174"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="76"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="107"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW30180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="562" mass="65362" checksum="06BE4C3FA0DFF3E4" modified="2011-11-16" version="1">MNYLSNYQKSRILDVAYDNQLMPVNIANNFYQILGLIALEIDVPGSADYVSLQFRLNNLPELPRKFVQSIIEENEDDEGGHFTDPLHAIGNSTDGGEWKRRNSGAAQDDDNDDDTDDPLLADHTSSHSLIDETEAEGLKPTGQSRTDSGNIIDSALIDNYVAEIRDKFKPLLEGNDQVRIKEVPEKEGIIFKHINYIITFESGGHSKKVIRRYSDFVWLLDFLLQKYPFRVIPGLPPKKFTVGSSPDSQFLQRRRRGLHRFLNQLIKHPVLSEEPVVQTFLSVPTDITTWKKQAKIDYSLEFKGEKIGPNFINVIWPKIGVEFLKNWKQAETNVNKLIEIWTKVVLLVERHEKRQQQISFDNGKFVEMLSKFRELDTNVYPNSNETLLHDNDIGNVNDSLGYISEYFNKSSQILIDESYSINTNILEKFKNYLDYLYSLQELFDRTKRLSINTIEQLEAKIRENETKFKKLSTEDADAKGADITKLRQIIINDKQEIFQQLNKDWLIKQCCFQEFVSFQETQFLIGELWIEWCKGRYRFEEKIVGLYDNLNREIINDMPTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVY1</accession>
    <name>G3AVY1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_53063</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007378008.1">
        <property type="nucleotide sequence ID" value="XM_007377946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVY1"/>
    <dbReference type="GeneID" id="18871751"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53063"/>
    <dbReference type="eggNOG" id="ENOG502QWCT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1240796_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVY1"/>
    <dbReference type="OrthoDB" id="4050369at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="223" mass="25402" checksum="1C5FFECF07A985AF" modified="2011-11-16" version="1">MYLVRTVDGVKLEEANPGGCIKVPVYKLSMQGSKYHIVYIAVPNDDSISKDNHMQVDFSTVDTSNNNSYLSGLCEGVELTAEHISLLKKLDQSPTSFNGEEFYTKVYDNVISKPTSRCYYVPKGHNSMTLSPFFNVHTSNVKKVYTRYFGYLYAILVNYDKFDFMNTAAEGKARELLESVKYADTFDGRYITILELFDYFMMENDNILNLALLPFLLDLKPTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADX4</accession>
    <name>G3ADX4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycogenin glucosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63932</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372110.1">
        <property type="nucleotide sequence ID" value="XM_007372048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ADX4"/>
    <dbReference type="STRING" id="619300.G3ADX4"/>
    <dbReference type="GeneID" id="18874950"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63932"/>
    <dbReference type="eggNOG" id="KOG1950">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017171_4_0_1"/>
    <dbReference type="InParanoid" id="G3ADX4"/>
    <dbReference type="OMA" id="VPKLFPW"/>
    <dbReference type="OrthoDB" id="2014201at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="366"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="458"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="600"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="644"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="611"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="694" mass="81533" checksum="56D577354BDB934F" modified="2011-11-16" version="1">MTQAIFTLLYNPSYLAGALVLGSTLKKLIQKQEDGSELKLGILIDKKNFTSHHLALLSKYYDDLVDVDPLESKITDKLYHDLKRPELGKTFTKVKLWSLIQYEKILYLDSDTLPIVPTGDQGSVIDLLKLDFAKNKILAAPDSGFPDIFNSGVFVLKPNLDDYSKLDALVQESAINPNVSFDGADQGLLNQYFNTQPDWVQSLLKAGKSNIQQAESTAETNWVKIPFLYNVTPSAQYEYLPAYKHFSTSPEYPGAPTPGPLEAELGEGGQDQDKHDEEVVRSTFETLSRYHSTAVTYINYSTTQVKVIHFIGPYKPWRSSSTVSGIHRDWWNIWIQEFGERSIQEVISPPASEAFVYEEPEQVYHHEESWDVPHPPPPPPPEPVYVPEPEPIDHSQPQSLLDPANYQQFEDNIKPSVDAMWDPTKEAPPVHEYHFDGSHEMEHGMRSFTNVWDEPYHEPEHHHEEHHHHHHHEEPQWHHHEEHHHHEEHHHEEPQWHHHEEHHHEEPQYHHHEEHHYEEPHHEEPQYHHHEEHHYEEPHHEEYHEPHHEEHHHHEEHHEEHHEEPQYEAPVPVYDHHYAQPERVFDTKYDYFPTHVLQQLDKSTSTEEPRSLSSPSAESSDNAATNFINFAEVNQELQRSGVIDEDDFEEDYGELEEELPEEEEYEEEIEEDYNVPKLFPWEFRPAYRAERVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE25</accession>
    <name>G3AE25_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CENP-V/GFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_58787</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfa family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372971.1">
        <property type="nucleotide sequence ID" value="XM_007372909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE25"/>
    <dbReference type="STRING" id="619300.G3AE25"/>
    <dbReference type="GeneID" id="18872607"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58787"/>
    <dbReference type="eggNOG" id="ENOG502SAKJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055491_3_2_1"/>
    <dbReference type="InParanoid" id="G3AE25"/>
    <dbReference type="OMA" id="RCGCTLW"/>
    <dbReference type="OrthoDB" id="4001640at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1590.10">
        <property type="entry name" value="glutathione-dependent formaldehyde- activating enzyme (gfa)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006913">
        <property type="entry name" value="CENP-V/GFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33337:SF30">
        <property type="entry name" value="DUF636 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G03180)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33337">
        <property type="entry name" value="GFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04828">
        <property type="entry name" value="GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51891">
        <property type="entry name" value="CENP_V_GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CENP-V/GFA" evidence="5">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="134" mass="14899" checksum="EDDD8B8F67FC4A0A" modified="2011-11-16" version="1">MSYTGSCLCGDVTFKLNGEPKRTITCYCTDCRKGSGALGQFISVYDTTNVEIKDPNSKVGEYVVQKTQSGTPKKKQFCTGCGCTIRTLPGKYKGEVSMMRQALLDDDFKNHVPQASIFNDEKERFTSGIKSEFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE31</accession>
    <name>G3AE31_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ribonuclease III</fullName>
            <ecNumber evidence="3">3.1.26.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_58792</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ribonuclease III family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.3" evidence="3"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372977.1">
        <property type="nucleotide sequence ID" value="XM_007372915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE31"/>
    <dbReference type="FunCoup" id="G3AE31">
        <property type="interactions" value="278"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AE31"/>
    <dbReference type="GeneID" id="18872608"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58792"/>
    <dbReference type="eggNOG" id="KOG1817">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026251_1_0_1"/>
    <dbReference type="InParanoid" id="G3AE31"/>
    <dbReference type="OMA" id="MIIYNKF"/>
    <dbReference type="OrthoDB" id="2392202at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034963">
        <property type="term" value="P:box C/D sno(s)RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030847">
        <property type="term" value="P:termination of RNA polymerase II transcription, exosome-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19876">
        <property type="entry name" value="DSRM_RNT1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000001">
        <property type="entry name" value="Ribonuclease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00104">
        <property type="entry name" value="RNase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040540">
        <property type="entry name" value="RNase_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011907">
        <property type="entry name" value="RNase_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044449">
        <property type="entry name" value="Rnt1/Pac1_DSRM_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207:SF0">
        <property type="entry name" value="RIBONUCLEASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="RIBONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18497">
        <property type="entry name" value="RNase_3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="11">
        <location>
            <begin position="178"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="10">
        <location>
            <begin position="333"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="415"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="415"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="427"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW35565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="532" mass="59985" checksum="4692AAD4B9F601F9" modified="2011-11-16" version="1">MVQITDTSNENSEQSSSSKKRARSPSDLDMYNRPPAKTPTFDREIPKSVAFVDLQKLEYATRTLQKNVETVLELAPDVLSLKSLIDAPNLNQGIKMELKQNDLIEMASKLQSRYKIGTLPILDEITSGKINFNDKDMKKLKKCDPNTQNIQVEVPVTPMTDEIAPRGINNTLPPLPQIRDPALYERVFIHKSYVNSKSYMNSEKLINNHNERLEFIGDAILNTLATMIIYKEFPTATEGQLSQIRADLVNNKTLCQFSLQYGLDKKLRARIEDSVLKSGDQKIYADIFEAYIGAVSTERGLDLSEVKNWLHKLYQERLRKLRVLYISEPINRDAKSKLYSTIGMADAYPQYETIQQGDGVKVDCIVHCVMKGEVIGVGQAPSSKDASLRAAMDALRNKEKLEKYFLQRKMIDRPAKQGRKEDKASKESTPIKQSSEGNTSFDMSLFPVKVDESEPIDTSATNRLYSEIGQRIGSHPEYIINKGEKNQQIVTLKIRGVEVAVASDLSKKKAMRRAAKALLDNQKALDEICKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEL3</accession>
    <name>G3AEL3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_57842</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372187.1">
        <property type="nucleotide sequence ID" value="XM_007372125.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEL3"/>
    <dbReference type="GeneID" id="18872218"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57842"/>
    <dbReference type="HOGENOM" id="CLU_2814046_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEL3"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="67" mass="7718" checksum="980B8A35F45E4A46" modified="2011-11-16" version="1">MQDVAVFSFFVTVARSFIFIDGRHKKELWNSKKSESPSTTLTGMYQINRQAQEVYMFITCIVIPIAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AER8</accession>
    <name>G3AER8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cell wall synthesis protein KRE9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_58898</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in cell wall beta(1-&gt;6) glucan synthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KRE9/KNH1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373106.1">
        <property type="nucleotide sequence ID" value="XM_007373044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AER8"/>
    <dbReference type="FunCoup" id="G3AER8">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AER8"/>
    <dbReference type="GeneID" id="18872667"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58898"/>
    <dbReference type="eggNOG" id="ENOG502S28F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063732_1_0_1"/>
    <dbReference type="InParanoid" id="G3AER8"/>
    <dbReference type="OMA" id="YYYFQIY"/>
    <dbReference type="OrthoDB" id="2432613at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042546">
        <property type="term" value="P:cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045328">
        <property type="entry name" value="Kre9/Knh1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018466">
        <property type="entry name" value="Kre9/Knh1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008659">
        <property type="entry name" value="Kre9/Knh1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28154">
        <property type="entry name" value="CELL WALL SYNTHESIS PROTEIN KNH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28154:SF1">
        <property type="entry name" value="CELL WALL SYNTHESIS PROTEIN KNH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10342">
        <property type="entry name" value="Kre9_KNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05390">
        <property type="entry name" value="Kre9_KNH1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442456" evidence="4">
        <location>
            <begin position="19"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Yeast cell wall synthesis Kre9/Knh1-like N-terminal" evidence="6">
        <location>
            <begin position="24"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Yeast cell wall synthesis Kre9/Knh1 C-terminal" evidence="5">
        <location>
            <begin position="165"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="270" mass="29122" checksum="D3E1F7CD8BF1CE7C" modified="2011-11-16" version="1">MKLQILTILVSLLAYVIADVDISKPSKGDKFSGSSGAASVDIVWKDDASDSSDPMSLKNVKTYTISLCTGPDARGSIQCLQPPLMPATALTSLKATVSIKQGLVPDGYYYFQIYSIFANGGDTIHYSPRFQLTGMSGTAGTLAVTMTGAVPDPQVQGFDAAANINSAWFTIPYTKQTGKTRYAPMQTQPGSKVTATTWTMRFPTSSVSYYTTKAKSPVVYSTITPGWDYTAESAVNYATVAPYPTNWYPASERVSKATLSSATKKKRWLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AET2</accession>
    <name>G3AET2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">V-type proton ATPase subunit D</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">V-type proton ATPase subunit d</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10 11">Vacuolar proton pump subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_132137</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Vacuole membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase D subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373175.1">
        <property type="nucleotide sequence ID" value="XM_007373113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AET2"/>
    <dbReference type="FunCoup" id="G3AET2">
        <property type="interactions" value="879"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AET2"/>
    <dbReference type="GeneID" id="18869639"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132137"/>
    <dbReference type="eggNOG" id="KOG1647">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069688_0_1_1"/>
    <dbReference type="InParanoid" id="G3AET2"/>
    <dbReference type="OMA" id="REEFFRM"/>
    <dbReference type="OrthoDB" id="1888931at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.3240:FF:000002">
        <property type="entry name" value="Vacuolar atp synthase subunit d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00271">
        <property type="entry name" value="ATP_synth_D_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002699">
        <property type="entry name" value="V_ATPase_D"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00309">
        <property type="entry name" value="V_ATPase_subD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11671">
        <property type="entry name" value="V-TYPE ATP SYNTHASE SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01813">
        <property type="entry name" value="ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW35763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="278" mass="31132" checksum="AAF18AE75BDC902E" modified="2011-11-16" version="1">MSGAGNREQVFPTRMTLGLMKGKLKGAQQGHSLLKRKSEALTKRFRDITQRIDEAKRKMGRVMQTAAFSLAEVSYATGENISYQVQESVQRARFQVKAKQENVSGVYLPTFESYINEDINDFKLTGLGRGGQQVQRAKLVYTKAVETLVELASLQTAFIILDEVIKVTNRRVNAIEHVIIPRTENTISYINSELDELDREEFYRLKKVQEKKQEAAAAAEAEEKAAQEAAIAAGAELSKITRVTSNEEITNDAQEVEKLALANGDDDLLKENEDDVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEZ1</accession>
    <name>G3AEZ1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase class V domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_53204</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372207.1">
        <property type="nucleotide sequence ID" value="XM_007372145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEZ1"/>
    <dbReference type="STRING" id="619300.G3AEZ1"/>
    <dbReference type="GeneID" id="18871768"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53204"/>
    <dbReference type="eggNOG" id="KOG1549">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_3_0_1"/>
    <dbReference type="InParanoid" id="G3AEZ1"/>
    <dbReference type="OMA" id="EFAHHDG"/>
    <dbReference type="OrthoDB" id="5978656at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990411">
        <property type="term" value="F:hercynylcysteine sulfoxide lyase activity (ergothioneine-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903257">
        <property type="term" value="P:selenoneine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43092:SF2">
        <property type="entry name" value="HERCYNYLCYSTEINE SULFOXIDE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43092">
        <property type="entry name" value="L-CYSTEINE DESULFHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="2">
        <location>
            <begin position="33"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="422" mass="48351" checksum="36927A7AFC523B7A" modified="2011-11-16" version="1">MTEKFGKQFREKYFTSIDPEVYPVNHGSWGQAPDPVIQKFKQASGEQYHYTDKFFHDIRDKYTDALKQLSEVVNCDYHNLALVDNATTGVNTILRSYPFKKGDKIVMQSNVYGSCYNVVKFLNYRYGVEYVEIEVNYPISDDEILQKFEKVFKEEKPTMCLFDTISSMPGVIFPFKQLVNLCKKYDVVSLIDGAHGIGCIPQDLSEIAPDFYVSNLHKWFFVPTACALMYVDAKQHHKIHTMPISHSYVQDDVKLSEEQELNRLIDRFSFIGAKNYASVSVISDAIKFRNEVCGGEETIYKYCNSLAKQVGEVISKQWGTSYLDQENLISAMVNVEVPCESLGIDVAAIKANWTRFDQTVYTPSIDVHKSFIPCVVHNGKLYARFSCHVYNELSDYQHAADVLVKVMKDSLELEPLFKKLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF62</accession>
    <name>G3AF62_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">6-phosphofructo-2-kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_145399</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372263.1">
        <property type="nucleotide sequence ID" value="XM_007372201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF62"/>
    <dbReference type="FunCoup" id="G3AF62">
        <property type="interactions" value="157"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF62"/>
    <dbReference type="GeneID" id="18870684"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_145399"/>
    <dbReference type="eggNOG" id="KOG0234">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006383_0_2_1"/>
    <dbReference type="InParanoid" id="G3AF62"/>
    <dbReference type="OMA" id="VKTHVFH"/>
    <dbReference type="OrthoDB" id="267323at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000644">
        <property type="entry name" value="GpmB, Fructose-2,6-bisphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF39">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE_FRUCTOSE-2,6-BISPHOSPHATASE YLR345W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="4">
        <location>
            <begin position="86"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="544" mass="62300" checksum="74C97942E759CE4C" modified="2011-11-16" version="1">MSSSAISDDEGELYNGFGSSPISRNQMRTTKRWSFHNNTRPLMDLPVDANTGGKPRRLSDITGEDGVHKTLNPYSEWGGAQLYSTESGRLFHAGKIIIALAGLPGRGKTHLSVSLTRYLRWLGVKTHSFHLGDYRRATAEDNNLTHDLFVPTPSSKQAHAVREKAVNECLNDILNFFKADKGQVAIYDAVNAIPEFRLELHKKFTELNIQVLFIESLVTDDKIIQKNIEEAARLSPDYTNWDYEKAKKDYSERINALAPYYQPMGTEESQLSYIKFVNFGERIELHNSSYGYLINKVVFYLMNSRIKSGSVFFARCYKNTMDYASDPPLDAEGIQYAKNLTLTLVEHLKYCGKDYIGEGSILRPAELKRELSVDERQPPTGVDGVDKDSLVIWTSVKKRTIATTQFFTKRNIKVRHRIQLSQKNPGIVGSMSESEIEEKYPEFYQQYEQDPYHYRFPRAESYHDLAIKIEPLILEMERMSGDILIIADETILQVFYGYLMSCSCYEIPSLNFKEDEIIEIKFNAYSNSAERIKIKDFANTLNVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFH2</accession>
    <name>G3AFH2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Octanoyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.181</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_132353</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>octanoyl-[ACP] + L-lysyl-[protein] = N(6)-octanoyl-L-lysyl-[protein] + holo-[ACP] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78463"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="EC" id="2.3.1.181"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the LipB family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.181" evidence="5"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372373.1">
        <property type="nucleotide sequence ID" value="XM_007372311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFH2"/>
    <dbReference type="FunCoup" id="G3AFH2">
        <property type="interactions" value="415"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFH2"/>
    <dbReference type="GeneID" id="18869653"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132353"/>
    <dbReference type="eggNOG" id="KOG0325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035168_0_1_1"/>
    <dbReference type="InParanoid" id="G3AFH2"/>
    <dbReference type="OMA" id="FEMCGLP"/>
    <dbReference type="OrthoDB" id="19908at2759"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00592"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033819">
        <property type="term" value="F:lipoyl(octanoyl) transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000544">
        <property type="entry name" value="Octanoyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020605">
        <property type="entry name" value="Octanoyltransferase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00214">
        <property type="entry name" value="lipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10993:SF7">
        <property type="entry name" value="LIPOYLTRANSFERASE 2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10993">
        <property type="entry name" value="OCTANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016262">
        <property type="entry name" value="LPLase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01313">
        <property type="entry name" value="LIPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="9">
        <location>
            <begin position="96"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="6">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="141"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="211"/>
            <end position="213"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="224"/>
            <end position="226"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="8">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR016262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="291" mass="33003" checksum="DEC4E267E005A38C" modified="2011-11-16" version="1">MRVSVQLLQEASSRICPITLKPKKKFWPIRESYGTLRHIHFPGITSFQHGLDIQQALVNHNLHFKSTEFNIRKIQKTRGDTLTYKETQLIDKILDLKPLPTILTFEFNNVYAGGLRSKEEVSPELREQYEKSGCEFHQLDRGGQVTWHGKGQLVAYSVIDIKAFHNLTAKCFVTSILLESVKRTLLNNFGLKSEVIENPGVWTSADDKIASVGIRIKRGISEYGISLNVNPDLKFMNSFIMCGLDGKRATSIMKELGETSATVEQVANMYVKEVAELLNMPKIEYIQANEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFL6</accession>
    <name>G3AFL6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cell division control protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_145753</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372417.1">
        <property type="nucleotide sequence ID" value="XM_007372355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFL6"/>
    <dbReference type="FunCoup" id="G3AFL6">
        <property type="interactions" value="506"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFL6"/>
    <dbReference type="GeneID" id="18870699"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_145753"/>
    <dbReference type="eggNOG" id="KOG2655">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017718_8_0_1"/>
    <dbReference type="InParanoid" id="G3AFL6"/>
    <dbReference type="OMA" id="RSNPMGS"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005619">
        <property type="term" value="C:ascospore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990317">
        <property type="term" value="C:Gin4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001400">
        <property type="term" value="C:mating projection base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032160">
        <property type="term" value="C:septin filament array"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010314">
        <property type="term" value="F:phosphatidylinositol-5-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000921">
        <property type="term" value="P:septin ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031107">
        <property type="term" value="P:septin ring disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000196">
        <property type="entry name" value="Cell division control 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="6">
        <location>
            <begin position="56"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="420"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="444" mass="50937" checksum="0771192A5041199C" modified="2011-11-16" version="1">MTSVEPEKQPAIQMPSSVKSPITYTPQDIKIIKKVLNGYVGFANLPKQWHRKSVRRGFTFNIMAIGESGLGKATLINTLFNREIVNHRDDEETVEEDTEDKEGGVAVKIRSTKAEIEEDGVKLKVSVITAPGFGESINNTDYWKPIVDEINSRFDSYLEAECKINRSTIVDDRIHAFLYFIEPTGHSLRALDIAMMKLVHEKVNLIPIIAKSDTLTDDEIVEFKQRILADIAHQGIKIFTPEAPGDDDEETVSNTQSIIDTFPFAVVGSTKEVQTPDGRTVRGRQYPWGVIEVDNEAHNDFVKLRQLLIRNFLEELKEHTANVLYENYRTEKLKKMGIEQDSTVFREFDPLAKQQEERALHEAKLAKMEAEMKSVFQQKVSEKEKKLQRSEADLFARHKEMKDKLTKQIKILEEKKVQLEKQRSLPQEPMVQPAQQKTRKGFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFP4</accession>
    <name>G3AFP4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_58166</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372445.1">
        <property type="nucleotide sequence ID" value="XM_007372383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFP4"/>
    <dbReference type="FunCoup" id="G3AFP4">
        <property type="interactions" value="113"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFP4"/>
    <dbReference type="GeneID" id="18872352"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58166"/>
    <dbReference type="eggNOG" id="KOG1204">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_11_1"/>
    <dbReference type="InParanoid" id="G3AFP4"/>
    <dbReference type="OMA" id="TDMSEWT"/>
    <dbReference type="OrthoDB" id="153074at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05367">
        <property type="entry name" value="SPR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000281">
        <property type="entry name" value="Uncharacterized oxidoreductase YIR035C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF8">
        <property type="entry name" value="BENZIL REDUCTASE ((S)-BENZOIN FORMING) IRC24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="251" mass="27446" checksum="9D8074AC738ECD03" modified="2011-11-16" version="1">MTNVTIVTGASRGIGRAIVNILLKNPENKVIAVARTKEALESLQKQYGDRIEIVAGDITESSTSVEAVKLAESKFGQLDAVIANAGVLDPVGPIEKHNINEWKRLFDINFFSVVELIQQSLPALRKTHGKFIAVSSGASLKPYSGWYAYGSSKASLNHLIASLASEESEIQAISIAPGVVDTEMQQDIREKFGKDMSTEGLQRFIDLHQNKQLSPPEVPAAVYANLALRGWSNDINGKYLRFDDDVLKSYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFR7</accession>
    <name>G3AFR7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Urea active transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">DUR3</name>
        <name evidence="10" type="ORF">SPAPADRAFT_53408</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372468.1">
        <property type="nucleotide sequence ID" value="XM_007372406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFR7"/>
    <dbReference type="FunCoup" id="G3AFR7">
        <property type="interactions" value="845"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFR7"/>
    <dbReference type="GeneID" id="18871782"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53408"/>
    <dbReference type="eggNOG" id="KOG2348">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010778_2_1_1"/>
    <dbReference type="InParanoid" id="G3AFR7"/>
    <dbReference type="OMA" id="LGANWLT"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015489">
        <property type="term" value="F:putrescine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
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        <property type="term" value="F:urea transmembrane transporter activity"/>
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        <property type="entry name" value="SLC5sbd_DUR3"/>
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        <property type="entry name" value="Na/Glc_symporter_sf"/>
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        <property type="entry name" value="Na/solute_symporter"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="254"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="291"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="352"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="396"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="427"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="456"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="495"/>
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        </location>
    </feature>
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        <location>
            <begin position="585"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="624"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="692"/>
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        </location>
    </feature>
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        <location>
            <begin position="701"/>
            <end position="716"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EGW35056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="716" mass="78508" checksum="CB7C2150A4FA9356" modified="2011-11-16" version="1">MSESVVPLPQSAGYAVVVGLGFVFAAGMMLTTYILRRYNREIMTAEEFATAGRSVKTGLISAAVVSSWTWAATLLTSTTQVYNNGVSGGFFYAAGATCQITLFACLAIKGRERAPAAHTYLEIIKGRYGKVTHFVYIFWGFATNILVTAMLLTGGSAVVSDLTGMHAVAACLLLPLGVVIYTLFGGIKATFLTDYAHTVVMVVIIIIFAFTTWATSDKLGSPAAVWEIVTKLAETQPREGNAGGSYLTMHSRSGGIFFVINIVGNFGTVFLDNGYFNKAFAANPASTYRGYILGSLAWLPIPAFCSLTLGFAALALEHTEHWPLGRPMTAAEVNSGLVLPNAASALLGKGGAVLSLLMVFMAVTSAMSAELIAVSTICTYDIYRTYINPQASGKKLIWVSHMSVIGFAYIMAGFAIGLYYAEVSMGYLYELMGVIIGGAVLSSALTLLSKRQNWHAATFTPPIATALAIMSWLVVTKVQFGAITYDNTFEDNAMLTGNCVALLSPLVLIPAFTLIFKQQNFDWEILKTKIFRVDEEEELAEATKVTIVDDERITPIKSQISVIASQLVEKEIDGYEHEQQVLHRAFFKCCIVCVTLTICLLVLWPMPMFGTKYIFSKRFFTGWVVVFFIWIFYTVFQVIIFPIWEGRESLCNTFRGIYWDLSGQGYKVKEWQQQHPEKLHAVQSQLQAQLSNQKGEPVMSSAVTPARSYSSDVKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFY1</accession>
    <name>G3AFY1_SPAPN</name>
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        <recommendedName>
            <fullName evidence="2">PSP1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_146024</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007372470.1"/>
    </dbReference>
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    <dbReference type="eggNOG" id="KOG4679">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_412173_0_0_1"/>
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    <dbReference type="OrthoDB" id="243127at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047767">
        <property type="entry name" value="PSP1-like"/>
    </dbReference>
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        <property type="entry name" value="PSP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43830">
        <property type="entry name" value="PROTEIN PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43830:SF3">
        <property type="entry name" value="PROTEIN PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04468">
        <property type="entry name" value="PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PSP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSP1 C-terminal" evidence="2">
        <location>
            <begin position="453"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="170"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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            <end position="677"/>
        </location>
    </feature>
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        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="209"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="627"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="677" mass="76866" checksum="C0A2B60CDD1FC065" modified="2011-11-16" version="1">MQMNYPDFLNAESNRKSIGSNTSKDTSKGSPPSTISSTTTVSSNGTTNSSKSNEDPIRFNNMLFNGLSTLDETQAVDILSPPHARNYYNCFDNINPPKRDTFNLPRELQGGVNNISSRRPSYAAESFTRNTNFPAFGAPNLSIQQSPGEFDSQHQPPQVPKTANSSKFFNSNKSFTPSFSNQLPMVNTFSNPNAQFQYDQFSDNFANMNINTNFNNFQSSRPTQQVQQQQEQQPSQQGQQQSQQQSPGTQKLSLSPGSNNFPIRLENGLILKDKYIIASSDRLKSLFLKTMKYFQDPAITAEILTKLNALLMNPVIIKLVTFIKNLNNLTFNHKILCLVINKNGKFDLLSYPNNSNILLQKNDLVISDGDRGKDLVMIVEPLVNLDFAILFNFLKKVEHLKSLTIQDNAGKVGNRGQVSKSNGGTHSTLLNASSIITSHSNEDNEFIITLPTKQVLRFATPAEVHKISGKFLEEKKAFITCYNKIKELNLESNLTLINVEYQSDLKKLIFYYFAGFKRIDFRGLIKELFKIYKTRIWLCAVLPFDKPETYITMKKNQKVPEKNSEEDIIPREYDLSNEQILNFSINEFDNLASPNYFHSINLLNLIEHLNNELEGYFYGFNSDKKTTQPTTTPNQKQQLKQQQQQQSQQQGVSNEPYCVEPDRSRVLPYFDPFGDAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG40</accession>
    <name>G3AG40_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase ECO1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Establishment of cohesion protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_131666</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetyltransferase family. ECO subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372591.1">
        <property type="nucleotide sequence ID" value="XM_007372529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG40"/>
    <dbReference type="FunCoup" id="G3AG40">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG40"/>
    <dbReference type="GeneID" id="18869598"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131666"/>
    <dbReference type="eggNOG" id="KOG3014">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039183_2_1_1"/>
    <dbReference type="InParanoid" id="G3AG40"/>
    <dbReference type="OMA" id="PSITHQE"/>
    <dbReference type="OrthoDB" id="428854at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061733">
        <property type="term" value="F:protein-lysine-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140588">
        <property type="term" value="P:chromatin looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034089">
        <property type="term" value="P:establishment of meiotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034087">
        <property type="term" value="P:establishment of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007088">
        <property type="term" value="P:regulation of mitotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032200">
        <property type="term" value="P:telomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070058">
        <property type="term" value="P:tRNA gene clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028005">
        <property type="entry name" value="AcTrfase_ESCO_Znf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028009">
        <property type="entry name" value="ESCO_Acetyltransf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45884">
        <property type="entry name" value="N-ACETYLTRANSFERASE ECO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45884:SF2">
        <property type="entry name" value="N-ACETYLTRANSFERASE ECO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13880">
        <property type="entry name" value="Acetyltransf_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13878">
        <property type="entry name" value="zf-C2H2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="N-acetyltransferase ESCO zinc-finger" evidence="12">
        <location>
            <begin position="11"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase ESCO acetyl-transferase" evidence="13">
        <location>
            <begin position="170"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW35179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="245" mass="27027" checksum="1E4AB7BB1AC6C28D" modified="2011-11-16" version="1">MTGTKKKSQVQSILSFDSDKPHTCPVCEMTYNVSIPSEKQAHTKYHSSFVNGIPWTTALPSLDSFTLIVKSVKSGRSNTIVRGNIVTIDKTNKSQVSKVDQLLDMVNQELSASQDSGEWKSLKHESSKAFVIVIENRAVGVCSTDTVGDSLGRWMIHSSQTIVPNQINKSIKIGISRIWIAPKWRRYGLGQRFLQVVLKNSIYGVTLTKQQIAFSQPSTSGGLLAKSFNGVSHKSGEILIPVYIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG54</accession>
    <name>G3AG54_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">SPAPADRAFT_133040</name>
    </gene>
    <organism evidence="26">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372605.1">
        <property type="nucleotide sequence ID" value="XM_007372543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG54"/>
    <dbReference type="FunCoup" id="G3AG54">
        <property type="interactions" value="135"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG54"/>
    <dbReference type="GeneID" id="18869712"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133040"/>
    <dbReference type="eggNOG" id="KOG1027">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004875_2_1_1"/>
    <dbReference type="InParanoid" id="G3AG54"/>
    <dbReference type="OMA" id="QCYEKDY"/>
    <dbReference type="OrthoDB" id="63989at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990332">
        <property type="term" value="C:Ire1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990604">
        <property type="term" value="C:IRE1-TRAF2-ASK1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070059">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036498">
        <property type="term" value="P:IRE1-mediated unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09769">
        <property type="entry name" value="Luminal_IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10422">
        <property type="entry name" value="RNase_Ire1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13982">
        <property type="entry name" value="STKc_IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000572">
        <property type="entry name" value="Serine/threonine-protein kinase/endoribonuclease IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.180:FF:000002">
        <property type="entry name" value="Serine/threonine-protein kinase/endoribonuclease IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000443">
        <property type="entry name" value="Serine/threonine-protein kinase/endoribonuclease IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.180">
        <property type="entry name" value="KEN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045133">
        <property type="entry name" value="IRE1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010513">
        <property type="entry name" value="KEN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038357">
        <property type="entry name" value="KEN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13954">
        <property type="entry name" value="IRE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13954:SF6">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06479">
        <property type="entry name" value="Ribonuc_2-5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00580">
        <property type="entry name" value="PUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51392">
        <property type="entry name" value="KEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 22" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="17" id="KW-0834">Unfolded protein response</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442481" description="non-specific serine/threonine protein kinase" evidence="22">
        <location>
            <begin position="21"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="716"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="domain" description="KEN" evidence="24">
        <location>
            <begin position="1028"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="584"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="39"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="615"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="641"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="667"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="EGW35193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1158" mass="131381" checksum="EAFF02B814481ED3" modified="2011-11-16" version="1">MQILYSAITILVLFTSIVLSTPRRENAGYLTAQQVYNEQEPAPAQQPAQPQPNQQDSPSILPMDARSINDCDLTDLLLISDIDGHLYGVERNSGSLMWTLPIDEPLVKIQTNNSINNLQSNILWFVEPYQDGSLYYFTPKFGLNKLPTSIKDLVMESPFSLSGDDKIYTGTRKTSLYTINIHTGEIKSSFGSDEKCPVPNTKVPLGDSTSSQYQDDTVMIGKTTYELAIHSKSNSSIVWNVTYSQWVPNNIDNDLVLQNQQSLDKLYFTPFHDRSLLAINKDIGTPVWISKLPSLAVNIFDIFNNGKTGDYILLPHPLKVLNNLQIKEHHPTNNDLVFVNKTSNSQQWIAMSFENYPTLIKSAPISNYQTMLNNYYGGVTQPLDYLESFHVVKASESKVEKLISGIHRAFQLSSDTSYQPIARYTTAQEQIKRIGDGNGDDTIEQQSSKFPGIMDGIKFVEKLPPSFESDVYLLEASDQYEALPQAPVTKTIGGGVNSTAIVRRIIEDLAVLVVIFVLLMTFGKSNRLLRKVLGEYRFIQDDQESRAQQVLNKQEVKPPSTIVVEGMAMEKVHGIKDDVVEKTEVVAPSSDSSNDEHQEVEPKKKKVVIVEPEEQNGTAESTTEGTVEPESDDAESTPAIKKPRKRGKRGGKRGGKHINKNKSNEDEHNEDDDTEQQMEVEEDEEPVITTKSLVKSIPMTPSKSTKKKLQIENNLVISDKILGYGSHGTVVFEGTFENRPVAVKRMLLDFYEIANHEVRLLQESDDHPNVIRYFCSQTSESEKFLYIALELCLCTLEDIIEKPKKSPELLIPKKNDILYQLASGLHYLHSLKIVHRDIKPQNILVADIKKTKHNQQLQNGNVPSEHENNVRLLISDFGLCKKLDNDQSSFRATTQHAASGTSGWRAPELLLNQDLLEISPDSISSIHSQNNSTTQSTSSGKRLTKAIDIFSLGCVFFYILTGGSHPFGDRYLREGNIIKGDYDLTLLKIRCPNDKFEATDLISSLINHEPALRPNTSKILKHPLFWSSGKRLEFLLKVSDRFEVERRDPPSELLLKLEEKARKVHNGDWHKRFDTEFMDNLGKYRKYNKDKLMDLLRAIRNKYHHFNDMPESLQSKMSPLPDGFYKYFNDKFPHMLMEIYYVIEENLKHEHVFTEYYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGF2</accession>
    <name>G3AGF2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein S12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_133056</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372704.1">
        <property type="nucleotide sequence ID" value="XM_007372642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGF2"/>
    <dbReference type="FunCoup" id="G3AGF2">
        <property type="interactions" value="383"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGF2"/>
    <dbReference type="GeneID" id="18869715"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133056"/>
    <dbReference type="eggNOG" id="KOG1750">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104295_0_1_1"/>
    <dbReference type="InParanoid" id="G3AGF2"/>
    <dbReference type="OMA" id="HYDPIKP"/>
    <dbReference type="OrthoDB" id="148331at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03368">
        <property type="entry name" value="Ribosomal_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000099">
        <property type="entry name" value="Ribosomal protein S12, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005679">
        <property type="entry name" value="Ribosomal_uS12_bac"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00981">
        <property type="entry name" value="rpsL_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01034">
        <property type="entry name" value="RIBOSOMALS12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="19694" checksum="06FA9B31AA094AF9" modified="2011-11-16" version="1">MFRSLLASGARLSSSLMMRQNPIVSKPITSYMKSISSNIPQSIFNSTMSIRHATINQIKYGKQQKPVKQTLSKSQELQKNPFKKGVVVRIMILKPKKPNSAMRKCCRVRLSNGKVISALIPGEGHNLQEHHIVLVRGGRVQDLPGVKYRLVRGAFDLAGVANRGSSRSKYGVKKPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGN2</accession>
    <name>G3AGN2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_58594</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372783.1">
        <property type="nucleotide sequence ID" value="XM_007372721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGN2"/>
    <dbReference type="FunCoup" id="G3AGN2">
        <property type="interactions" value="963"/>
    </dbReference>
    <dbReference type="GeneID" id="18872522"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58594"/>
    <dbReference type="eggNOG" id="KOG1278">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010714_4_0_1"/>
    <dbReference type="InParanoid" id="G3AGN2"/>
    <dbReference type="OMA" id="KVYYMFG"/>
    <dbReference type="OrthoDB" id="1666796at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF111">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="229"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="335"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="382"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="421"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="459"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="491"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="527" mass="59504" checksum="E1961619BE533354" modified="2011-11-16" version="1" fragment="single">MLENKECERLCTSKYSKSDSVFVNRNIRASYNHNWIIDGLPVASVVKDERTDTTFYGPGFHIGEVDSKNNAHLSNHFVITIEYHKRGEDKFRVVGATAERHSLDRTGVASDASNDKVCSLDLNPVQLSKEKESDVLFTYSIQFKESDVAWATRWDKYLHVYDPKIQWFSLINFSLIVSILGIIIGHIIVRTLKNDIVKYNEVNLDDDISDESGWKLVHGDVFRPPKQRLLLSVLVGSGLQIFLMSFITIGFALFGLLSPSNRGSLSTFTFILYIGCSFVSSFTSAYLYKFFGGENWKLNMILSPVLVPGILFGVIVLLNFFLISVNSSGAIPMGTMVVIVIIWFLVSIPLSVLGTILASKKPLISVPVRTNQIPRQVPVQPWYLRTIPVMFISGIFPFGSIAVEMYFIYSSLWFNKIFYMFGFLFFCFLLMVITTGLITILMIYYTLCSENYKWHWKSLFIGGGCAIYVFIHSFFLIGGEKLGGFTSFVLYTGYSIVISLLVFLCCGSVGFLCSLAFVKTIYAQIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGU5</accession>
    <name>G3AGU5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein HGH1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_133103</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HGH1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372841.1">
        <property type="nucleotide sequence ID" value="XM_007372779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGU5"/>
    <dbReference type="FunCoup" id="G3AGU5">
        <property type="interactions" value="1109"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGU5"/>
    <dbReference type="GeneID" id="18869721"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133103"/>
    <dbReference type="eggNOG" id="KOG2973">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037769_2_1_1"/>
    <dbReference type="InParanoid" id="G3AGU5"/>
    <dbReference type="OMA" id="MCILLTN"/>
    <dbReference type="OrthoDB" id="76676at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061770">
        <property type="term" value="F:translation elongation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039717">
        <property type="entry name" value="Hgh1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007206">
        <property type="entry name" value="Protein_HGH1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007205">
        <property type="entry name" value="Protein_HGH1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13387">
        <property type="entry name" value="PROTEIN HGH1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13387:SF9">
        <property type="entry name" value="PROTEIN HGH1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04063">
        <property type="entry name" value="DUF383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04064">
        <property type="entry name" value="DUF384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein HGH1 N-terminal" evidence="4">
        <location>
            <begin position="95"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Protein HGH1 C-terminal" evidence="5">
        <location>
            <begin position="291"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="346"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="362" mass="41625" checksum="871E64BCC8CB4095" modified="2011-11-16" version="1">MPTELEELVSFLHSPQPAVVQIALDNLVGYSQSAHQQVFAYDNYTAITDLKNLSKSNSSTMVQQSITILANLCDDLIMRNLIVEDDDYLKYLCTQIINLKNVNCDLMCILLTNLAKNDNITKVLEFEIEKFDDEQKKHFSSTKIIDCLMDCFVKGYDRNLNKYANFDYLAYFFADLSRYTQGRSYFVTPQSYDNVVPLSKLLVFTEKYDAKIRREGVCSTIKNSLFDTNSHMRLLEDEQINLLPFILLPLAGPGEGIDEEDMFELPDELQLLPEDKQREPLNGVIAIHLESILLLCTTRQGREYLRNKSVYAIIKELHKDNNNEQVVDLCDRVVQMLKRDEAPVDAADEESDEEDDKIVEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGU6</accession>
    <name>G3AGU6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription initiation factor IIF subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_58648</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TFIIF alpha subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372840.1">
        <property type="nucleotide sequence ID" value="XM_007372778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGU6"/>
    <dbReference type="FunCoup" id="G3AGU6">
        <property type="interactions" value="163"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGU6"/>
    <dbReference type="GeneID" id="18872552"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58648"/>
    <dbReference type="eggNOG" id="KOG2393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020322_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGU6"/>
    <dbReference type="OMA" id="FEEFYPW"/>
    <dbReference type="OrthoDB" id="76676at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072542">
        <property type="term" value="F:protein phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001096">
        <property type="term" value="F:TFIIF-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001174">
        <property type="term" value="P:transcriptional start site selection at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008851">
        <property type="entry name" value="TFIIF-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011039">
        <property type="entry name" value="TFIIF_interaction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13011:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13011">
        <property type="entry name" value="TFIIF-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05793">
        <property type="entry name" value="TFIIF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50916">
        <property type="entry name" value="Rap30/74 interaction domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="44"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="202"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="344"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="429"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="202"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="346"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="367"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="555" mass="63133" checksum="F38695D61E9FA4D1" modified="2011-11-16" version="1">MKFISKQDVDIVDNFVKPVRLHRKDPRNIQFHLTRRELDIRKRENEELKREKQEQQDGEVPAENNGEVAPDKPMVTPGGANLAQVAPDGGARKQKKNLFKRKTRQINLMDDAKRKLRYEEHYPWVIEDYDGKNVFVGNYEAGSSESQHVLFVFDKDGFKMVPAEKVYRFTPRNKYATLTLEEAEAKMERNATVPRWLMKHMEDRSTSDTIPDQRFRNSSAANGGSTFNRAIQPGPSSSRPRLRTVIGGSASRESNDRDSDHDDLDYDEEFADDEEAPIMDGDEEENKLSERKIKKEMLKAAHFDGKSDVEGDLDDLDDLFETEKTRKVDKEGKKLRKVLNKREGGVYDDDDDDEEGLLPYLSKSDLESNDESDDEDEVVVKKEDEVKLENNQTAQHYHRTIYVHHIDSGYVTIKAPASFLSTLPKGEWNPQVRRRPTPDITSPKKKIKLEEKSESPLTDIAASPTKSPSPIDTATTTTTTTTTTTTIDLNSSGPDNVLVTVNDVLDIVRNNPLTTKELLVGLKSRVSAHKDNKQRIITIVKQNLKLVDGKLVLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGW6</accession>
    <name>G3AGW6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_133106</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372051.1">
        <property type="nucleotide sequence ID" value="XM_007371989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGW6"/>
    <dbReference type="FunCoup" id="G3AGW6">
        <property type="interactions" value="131"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGW6"/>
    <dbReference type="GeneID" id="18869722"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133106"/>
    <dbReference type="eggNOG" id="KOG2151">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033275_4_1_1"/>
    <dbReference type="InParanoid" id="G3AGW6"/>
    <dbReference type="OMA" id="IFYMKPR"/>
    <dbReference type="OrthoDB" id="25391at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="4">
        <location>
            <begin position="45"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="171" mass="19855" checksum="000F45189009B2C6" modified="2011-11-16" version="1">MNRYGLEGLTSLVRMDQSDLTNFAIGQDISKSGLDLSKDSEILKNLASPWAETSRSEVEPYFTLPPEIRSENIVPGPGPCDNKIQSFTDETLFYIFYMKPRDTLQEYAARELVARNWRYHKDIQVWLTKDSNVEPVLISQDVEKGVYIFFDPHNWEKIRKEFVLHYSSVQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGX1</accession>
    <name>G3AGX1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">beta-glucosidase</fullName>
            <ecNumber evidence="3 6">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">BGL5</name>
        <name evidence="8" type="ORF">SPAPADRAFT_57697</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Glycan metabolism; cellulose degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="3 6"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372056.1">
        <property type="nucleotide sequence ID" value="XM_007371994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGX1"/>
    <dbReference type="STRING" id="619300.G3AGX1"/>
    <dbReference type="GeneID" id="18872157"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57697"/>
    <dbReference type="eggNOG" id="ENOG502QR4D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004542_4_0_1"/>
    <dbReference type="InParanoid" id="G3AGX1"/>
    <dbReference type="OMA" id="FAEPPRN"/>
    <dbReference type="OrthoDB" id="47059at2759"/>
    <dbReference type="UniPathway" id="UPA00696"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000495">
        <property type="entry name" value="Periplasmic beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050288">
        <property type="entry name" value="Cellulose_deg_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019800">
        <property type="entry name" value="Glyco_hydro_3_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011658">
        <property type="entry name" value="PA14_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715">
        <property type="entry name" value="BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715:SF27">
        <property type="entry name" value="BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07691">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00758">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00775">
        <property type="entry name" value="GLYCOSYL_HYDROL_F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PA14" evidence="7">
        <location>
            <begin position="214"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="651" mass="71955" checksum="A958A87C08F4C708" modified="2011-11-16" version="1">MTAYNKVNGVHVSESKAILQGILRNEWKWDGCTMSDWYGTYSTKDSLDAGLNLEMPGPTRFRQVLQTVHQVQANLIHPKVIDDNVRQVLKLINQGLKAGIPADVIESPNNSPESSKTLRKVGDESIVLLKNEESILPLSKTAKETIAVIGPNAKASQDSGGGSASLTARYKTTPYGGIEEKVKEGNNSIKLEYALGAFLDKNVPDSAAILESESGKKGLITARFYHTAPGTPDRKPFDELSLNTTRIFLNDFKSDNLSLEQLLYYADFSGYFTPEESSEYEFGCSTFGSAQVFVDGKLVVDNKTKQVKGDAFFLAMGTREERGSIYLEKGKKYLVKVEFGTSPTYTLANGSQEPGGVYFGVRIKSSPEDELAKAVKVAKSADKVVLVVGLSKEWESEGFDRPDMDIPGYTNKLVEEVAKVNPNIIVVNQSGSPVTLPWVDKTKALVHAWYGGNELGNTIADVLFGDYNPSGKLSVSFPKRLEDNPSFVNFGSNKGQVWYGEDVFVGYRFYEKVGREVLFPFGFGLSYTSFDFKNLKVDTTEDSLVVKVDVTNTGKVDGAEAVQLYIEQANPSINRPVKELKDFEKVFLKAGETKTVELEVSIKEATSYWNSFVNQWQSDKDTYKVLVGNSSDNILVKDEFTTKKTFTWSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH22</accession>
    <name>G3AH22_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Superoxide dismutase [Cu-Zn]</fullName>
            <ecNumber evidence="7">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SOD1</name>
        <name evidence="9" type="ORF">SPAPADRAFT_146717</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="7">Binds 1 copper ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the Cu-Zn superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="7"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372864.1">
        <property type="nucleotide sequence ID" value="XM_007372802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH22"/>
    <dbReference type="FunCoup" id="G3AH22">
        <property type="interactions" value="817"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AH22"/>
    <dbReference type="GeneID" id="18870724"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_146717"/>
    <dbReference type="eggNOG" id="KOG0441">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056632_4_1_1"/>
    <dbReference type="InParanoid" id="G3AH22"/>
    <dbReference type="OMA" id="AQRGFHI"/>
    <dbReference type="OrthoDB" id="2015551at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902693">
        <property type="term" value="C:superoxide dismutase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016670">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006825">
        <property type="term" value="P:copper ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901856">
        <property type="term" value="P:negative regulation of cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00305">
        <property type="entry name" value="Cu-Zn_Superoxide_Dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000001">
        <property type="entry name" value="Superoxide dismutase [Cu-Zn]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018152">
        <property type="entry name" value="SOD_Cu/Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00068">
        <property type="entry name" value="CUZNDISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00087">
        <property type="entry name" value="SOD_CU_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00332">
        <property type="entry name" value="SOD_CU_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6 7" id="KW-0186">Copper</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="8">
        <location>
            <begin position="14"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="154" mass="16092" checksum="D3CD589439FD6113" modified="2011-11-16" version="1">MVKAVAVLRGDSKVSGVVHFEQASESEPTTITYEISGNDPNALRGFHVHQFGDNTNGCTSAGPHFNPFGKTHGAPEDDERHVGDLGNITTDAHGVAKGTKQDLLIKLLGKDSIIGRTVVVHEGTDDYGKGGFEDSKTTGHAGGRPACGVIGLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH63</accession>
    <name>G3AH63_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_58735</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372905.1">
        <property type="nucleotide sequence ID" value="XM_007372843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH63"/>
    <dbReference type="FunCoup" id="G3AH63">
        <property type="interactions" value="108"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AH63"/>
    <dbReference type="GeneID" id="18872578"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58735"/>
    <dbReference type="eggNOG" id="KOG1737">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007105_4_0_1"/>
    <dbReference type="InParanoid" id="G3AH63"/>
    <dbReference type="OrthoDB" id="1854502at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035621">
        <property type="term" value="P:ER to Golgi ceramide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000742">
        <property type="term" value="P:karyogamy involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030011">
        <property type="term" value="P:maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13289">
        <property type="entry name" value="PH_Osh3p_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000013">
        <property type="entry name" value="Oxysterol binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041680">
        <property type="entry name" value="PH_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF203">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15409">
        <property type="entry name" value="PH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="618" mass="70579" checksum="CD0C083E65F02F16" modified="2011-11-16" version="1">MQGFTKRYFILNFNNGTLSYFRVKDNILRGQMPIKESIISANPSSREFIIDSGMEVWHLRAINNADFKAWVDAFNSIKQEVLKRKEEEVKSIKSEVEKPLLVELQELQEKLALLKASQPSNLIDEITQDLSSIIGRSDRESPPAASNGYDERSILSSEFYDAMETFEDYGVVIIEDEGEKSVPEDEFVDNLSVSSSEEEELDLVETPRHALEAATTITESTEEEDLYPLPHDPVARDSDIPICTHSPPSIISFVRKNVGKDLSALAMPVTYNEPTTALQKYAEVIEYPELITNALTHFSDQQGEKILRVATFALSYLSAVRSKERNKRKPFTPLLGETYELVREDLGFRMISEKVFHKPPVFVHYVESEHWTLKYALSPTQKFWGKHSELTTKGTVMLTDKATGEVFTWTHPTMLVKNIIAGETYAEPSGTMTIKSTSGYKAVAEFSKGGMFSGRSEGVVVKAFDHEKKEVPYTVTGKWTESFTLKTNTTEKCIWEAGDLLPQAEKKFGFTAFAGSLNKITEMERGQLAPTDSRLRPDLQQFEKGNVDEAEELKLKLEQDQRVRRKELEDKKEDHVPKFFRFSGNGEIDEGEWEYIQGEKSYWNRRKNQDWDDLLKLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH91</accession>
    <name>G3AH91_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nuclear rim protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_58753</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Member of a perinuclear network that controls recombination at multiple loci to maintain genome stability. Required for rDNA repeat stability.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NUR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372933.1">
        <property type="nucleotide sequence ID" value="XM_007372871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH91"/>
    <dbReference type="GeneID" id="18872591"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58753"/>
    <dbReference type="eggNOG" id="ENOG502S7S0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038615_0_0_1"/>
    <dbReference type="InParanoid" id="G3AH91"/>
    <dbReference type="OMA" id="TRSHIFQ"/>
    <dbReference type="OrthoDB" id="3363151at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043007">
        <property type="term" value="P:maintenance of rDNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007096">
        <property type="term" value="P:regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018819">
        <property type="entry name" value="Nur1/Mug154"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28293">
        <property type="entry name" value="NUCLEAR RIM PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28293:SF1">
        <property type="entry name" value="NUCLEAR RIM PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10332">
        <property type="entry name" value="DUF2418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="218"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="356"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="360"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="398"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="423"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="452" mass="52307" checksum="4C03E7B1E31B5859" modified="2011-11-16" version="1">MSKKKFIRRQSFISKIQSFPFDLYLYVNEVITSIDWNSVESIHIANTLGISLTLLDLAIVSLLNYYNSINSKSNNLLFKSNYYDYQYLKKHLSTSPKFTHYDVTASFTWVLSLLSNAITVASIANFILLIKSSRKYGLLYLTENNKSTTVYKTSTTDWLGFLDSLFVKLFGKEEDVTQSSIDQTHDTTVVEEDTYYMNTWDPNKFTLHLFITLNPLNIFIIHISSLLISLAVMVIQVTMSWLIISKFNQLIIDKQIIYQEMFKEYNNKFVNPKLNILKKDVAIDATKGPYDTTVLTDKQPYLLSKTKKFVTHDARGKEVVEYGNVDVPKFTIIDQSTLASFTTHDHDDLNFEERGEEPWYTSSTPYQPNSQKISQSTPNRLISPQRLNMSNRPVLGYNSESSRRNSFTSPSPVRMGVRSGTISPRRSPFRSPSPQRYDRYDPAYDRYYYDNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHF0</accession>
    <name>G3AHF0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alpha-1,3-glucosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_59537</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ALG6/ALG8 glucosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373698.1">
        <property type="nucleotide sequence ID" value="XM_007373636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHF0"/>
    <dbReference type="FunCoup" id="G3AHF0">
        <property type="interactions" value="962"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHF0"/>
    <dbReference type="GeneID" id="18872962"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59537"/>
    <dbReference type="eggNOG" id="KOG2575">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008110_2_1_1"/>
    <dbReference type="InParanoid" id="G3AHF0"/>
    <dbReference type="OMA" id="FQVPPMH"/>
    <dbReference type="OrthoDB" id="5589195at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042281">
        <property type="term" value="F:dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004856">
        <property type="entry name" value="Glyco_trans_ALG6/ALG8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413">
        <property type="entry name" value="DOLICHYL GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413:SF1">
        <property type="entry name" value="DOLICHYL PYROPHOSPHATE MAN9GLCNAC2 ALPHA-1,3-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03155">
        <property type="entry name" value="Alg6_Alg8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="238"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="273"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="346"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="376"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="442"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="468"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="498"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW34114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="526" mass="60879" checksum="79EB59EA1789B3EE" modified="2011-11-16" version="1">MAKPKKRTSSSKKDTFKEVSNDIPKVSSPKQDPPKSIFKDSPVYDLLHYFEKAPVQWEARYILVLTAVLLRSAVGLGGHSGYHTPPTFGDFEAQRHWMELTINLPISKWYYFDLSYWGLDYPPLTAYHSYLLGMIGSFINGSWFSLDESRGMETDGLRFFMRFMAIASELVLYIPAILTIANILGKKFNINRMDQIIISLVIINQPHLVLIDHGHYQFNSVMLGFFVYSVIDLIKNNLYLASIWFVSCINFKQMGLYYSLFIFFYILSQLKSFSGFILVGLTVAITQFVYLLPFILTKPESVLQIVLRVFPFNRGLFEDKVANFWCTTNVLIKYREIIDAAQLPKLALLATLLAVIPANVYLFFKIRSRPSTKIQGLIYGFSLNSLSFYLFSFQVHEKSILIALIPILLLLLIDHKDIDIIQWINNVGTFSLYPLLKKDELIMQYFVSNFLINWLIGPKILLQKKYSLNTVVVWGTYLLMIVYHVIDATMSPPTRYPDLWVILNIAISFAGFSYFWLWLVYKIYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHG2</accession>
    <name>G3AHG2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rhodanese-related sulfurtransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_54316</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373710.1">
        <property type="nucleotide sequence ID" value="XM_007373648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHG2"/>
    <dbReference type="FunCoup" id="G3AHG2">
        <property type="interactions" value="582"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHG2"/>
    <dbReference type="GeneID" id="18871851"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54316"/>
    <dbReference type="eggNOG" id="KOG1530">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089574_0_2_1"/>
    <dbReference type="InParanoid" id="G3AHG2"/>
    <dbReference type="OMA" id="YEGSWTD"/>
    <dbReference type="OrthoDB" id="566238at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01519">
        <property type="entry name" value="RHOD_HSP67B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE RDL2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086:SF10">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE_RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00380">
        <property type="entry name" value="RHODANESE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="34"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="133" mass="15123" checksum="B630E62F6E29AA9E" modified="2011-11-16" version="1">MYREKKSYSVLTESPEAKLYTYEDVKKLATNPESHPETVLVDVREPIEFSEGHIPGAINLPFKTSPGALELNEDDFLESFGFEKPSRDKELVFYCLGGVRSTAAEELARTFGYNKRGNYVGSYEDWVANENKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI00</accession>
    <name>G3AI00_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_54467</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373898.1">
        <property type="nucleotide sequence ID" value="XM_007373836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI00"/>
    <dbReference type="GeneID" id="18871865"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54467"/>
    <dbReference type="eggNOG" id="ENOG502RQ56">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_918803_0_0_1"/>
    <dbReference type="InParanoid" id="G3AI00"/>
    <dbReference type="OMA" id="SINKSEC"/>
    <dbReference type="OrthoDB" id="4080041at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="303" mass="36330" checksum="D3829CDAE73C1777" modified="2011-11-16" version="1">MKLQNSLPYQRKIRNYVERKDSLTTEYLSLREKIRMEDSPEHELENAFEVLLRLDELLYDYHVKRAYLEYSRIELVPENRLILEHIDRTIHKLERIVPIPLLVRSAPKLAIFRRQLFESAREAAKILAQTESSNPDAKKYISPESLEIHDHRAMRIRNLMRFEDGGWSKDHVEIIYDAARLKKYKSDRCEYIKTQFERKFKVNISIRTAMYLLTHYGFNDTNYRIKDFRKAFDQAYALHRDKLSQERTRYIIDTVKELIGIEMTKNDAQYGAKLRDIFTKVYGVPIEPPENCMEFITRKFEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI04</accession>
    <name>G3AI04_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Fructose symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">HXT4</name>
        <name evidence="11" type="ORF">SPAPADRAFT_59735</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373903.1">
        <property type="nucleotide sequence ID" value="XM_007373841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI04"/>
    <dbReference type="STRING" id="619300.G3AI04"/>
    <dbReference type="GeneID" id="18873055"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59735"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_5_1"/>
    <dbReference type="InParanoid" id="G3AI04"/>
    <dbReference type="OMA" id="MAIIVSC"/>
    <dbReference type="OrthoDB" id="6339427at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015798">
        <property type="term" value="P:myo-inositol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015791">
        <property type="term" value="P:polyol transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000134">
        <property type="entry name" value="MFS sugar transporter protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF9">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="118"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="423"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="457"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="483"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="80"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="545" mass="60373" checksum="86DFBD35E8478D44" modified="2011-11-16" version="1">MSGTISPTSEFDKPTVEKTENYSASVESVELGEDIDVDKEFGDYVQLGHELKSGGTFWTNLKNVEFPVTFKNRNNMVRFLGIFTSFAGILSGVDQSIISGASIGMNKALQLSDHENSLISSLMPLGAVAGSILMTPLSEHFGRKRAILISCILYTIGAIICAAAPDHETMYAGRFILGLGVGIEGGSVGVYIAECVPPTQRGNLVSLYQFFIAFGEVLGFAIAAIFFTVHGGWRFMVGSSLLFSTILLIGLLFLPESPRYLVYNNKYAEAINVYDRLRDISERTSKVEFLEMIKNAQAERKLRASQSRVQVWLDLFTIPRNRRALFYAVIMVSLGQLTGINAVMYYLSTMMANIGFSEENAVFMSLVGGGALWLGTIPAILYMDRFGRRVWGMNLVGFTIGLVLVGVGYLIPLETQRAAALGVYLTGLILYMGFFGAYACLTWVLPAESFSIGTRSIGMTICSSFLYLWAFTVTYNFNRMKSAMTYTGLTIGFYGGIAFLGFFYQIFFMPETKGKTLEEIDDIFEKPSMEIAKENLRNMKRFLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI59</accession>
    <name>G3AI59_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_134300</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373957.1">
        <property type="nucleotide sequence ID" value="XM_007373895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI59"/>
    <dbReference type="FunCoup" id="G3AI59">
        <property type="interactions" value="1132"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI59"/>
    <dbReference type="GeneID" id="18869801"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134300"/>
    <dbReference type="eggNOG" id="KOG1243">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010392_2_0_1"/>
    <dbReference type="InParanoid" id="G3AI59"/>
    <dbReference type="OMA" id="NDTSWAG"/>
    <dbReference type="OrthoDB" id="447103at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF3">
        <property type="entry name" value="N-TERMINAL KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="29"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="567"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="606"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="672"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="607"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="720"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="731" mass="82397" checksum="DD565CC8E63D6ADF" modified="2011-11-16" version="1">MNFFKQTLSSLTGSSIPYTIKEKVVDPQSLNPTDVERNSIWTVYDGINPKDNNPVSIFEFNLRDPANQIKTALARNAFKKLKMIKHPGVVSTVDFFDNDSSLYIVTEKVIPLLTYLQEEVSDDAKVNGVYQIAKTLSFINDSCHSSYLNLNIYNSVVVNAQGDWKLFGFELVSDADQTDVFAYKESMPGFREFAPPEENRTVKADSFRLGKFIFSVFDSSERNLPSRLVPIIKRLSGNNANVRITPTQYIKELEGWHGENPIIRFNEEIAELKFFSDADKLSFFRQQLPSYLQYETSFPVGFLNYKLLPELISQYNQLSKTAAGDSQKQETLCTMINLIIKFGIKLPPEDFHKTIKPIIVSCFCSTDRAVRLILLTHLPEYHSFFSDSEIQSQIFSNLITGFQDTNFMIRETTLKSITIIIDKISVKQVNQDLLRILAKSQMDPKPSIRVNTLILIIKISSKIYSNSRNNVLITALAKSLRDSFTPCKMAALHGFETLRGEFSMEEICSKVLGHLAIALMDKKSTKVRKEARRIFQVYLEAVESHAGDLDDYEEDEDAEEREFYKKFGPTVQQAEPEAAPTETTSNPEVQSLWGAVSKLASFGGSGQMNKDFNNSTPDLTSSVPSTPAAQPQSQVPILEQPVKADAWDTGDGWDDGDDGWNQNDEVEIVKETKKISISRKPQPIPTHKRPIGAKHSGLKLGAKVNKPKPKLQLNLKADDDKEVEDGWGDGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI75</accession>
    <name>G3AI75_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_70509</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DEAD box helicase family. DEAH subfamily. PRP16 sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373973.1">
        <property type="nucleotide sequence ID" value="XM_007373911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI75"/>
    <dbReference type="FunCoup" id="G3AI75">
        <property type="interactions" value="823"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI75"/>
    <dbReference type="GeneID" id="18875330"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70509"/>
    <dbReference type="eggNOG" id="KOG0924">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001832_6_3_1"/>
    <dbReference type="InParanoid" id="G3AI75"/>
    <dbReference type="OMA" id="CSLYDLW"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034458">
        <property type="term" value="F:3'-5' RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000386">
        <property type="term" value="F:second spliceosomal transesterification activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000378">
        <property type="term" value="P:RNA exon ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040031">
        <property type="term" value="P:snRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000007">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000615">
        <property type="entry name" value="pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF91">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE PRP16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="351"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="541"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="99"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="286"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="120"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="294"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="317"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1060" mass="120687" checksum="1AA5181F3C52A8DC" modified="2011-11-16" version="1">MDQDDIVTKITNKLSTYLNKPIHVNLTKSIISQFEKNNSFDPFAQFVKVLGISQDHANELFHDLDSELHGIIAKPKPKMRRASLEDELDLELAKPAAPKFKRMKKQDALRIKQETPTPSPAASPASPASPASPSANPRDSVKKTIHASNKEEYAPITPSSEESLDELETNIETEREWYTIDEEGTYARDDEFSENFTSSRQYKRTKVASTGYDPLTGEYIDTDHTADHDFSRIPIINHVFIPPFLQASQEYLQLQITGGSIRGIGPTVNPIKDSTSELAIMARTGSMTVQTRRTQKERALQAKERTGVSHESTTDANPDKQEKKPEDDTNRETILEQRRNLPAFAVKHDLITTIRDNQVTIIIGETGSGKTTQLTQYLYEEGFTNLNGVKQVIACTQPRRVAAMSVAKRVSEEMNCKLGQEVGFTIRFEDKTDPNKTIIKYMTEGILLREILVDPMLTNYSCIIIDEAHERSLNTDILLGLFRNLLMKRRDLKLIVTSATMNADKFTRFFGTAPQFTIPGRTYPVEVFFNKNTCSDYVEAAVKQILTVHLRNSSMSSGEYVNDGDILVFMTGQEDIEITCDLLREKLDLLENPPPLDIYPIYSTMPSDLQRKIFTKKNLRRRKVVVATNIAETSLTVDGIKYVIDCGLVKVKLYNPKLSMDTLQVVPISLANADQRSGRAGRVGPGITYRLYTERAIDSMYIQPIPEIQRTNLCNSMLLLKSLHIEDINRFPFLDSPPKDLLNCSLYDLWAIGALDNLGALTQLGKSMTKFPIEPTLAKLILLASRPEFHCSEEMITIVAMLSVPNIFSRPKERAKEADMARDKFVIAESDHLTLLNVYTQWNINMGKRINISAWCNKNFLQLKSLLRVKDIRKQLLLIMNSSKLPLLKSRSDDDIRKCLAASFYHQAAKLTKMNLSGNPEFVSLRHNYMKMYLHPTSSLLDSNLSTNFVIYHELVLTTKEYMNYITVVDPMWLLQFGYKFYTVADSYKDKINLPITKKNGRNDCTNTRFHNLKIIQGFRMTDCNIFNFKFWIGLDDWFNDIHDFLGWNRGFLVVVESFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI95</accession>
    <name>G3AI95_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_134208</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="15"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373248.1">
        <property type="nucleotide sequence ID" value="XM_007373186.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AI95">
        <property type="interactions" value="1153"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI95"/>
    <dbReference type="GeneID" id="18869790"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134208"/>
    <dbReference type="eggNOG" id="KOG0260">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000487_1_1_1"/>
    <dbReference type="InParanoid" id="G3AI95"/>
    <dbReference type="OMA" id="KPCMGIV"/>
    <dbReference type="OrthoDB" id="270392at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140463">
        <property type="term" value="F:chromatin-protein adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180034">
        <property type="term" value="P:co-transcriptional lncRNA 3' end processing, cleavage and polyadenylation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019985">
        <property type="term" value="P:translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02584">
        <property type="entry name" value="RNAP_II_Rpb1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02733">
        <property type="entry name" value="RNAP_II_RPB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.30:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.390:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.100:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.140:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.180:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.120:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000684">
        <property type="entry name" value="RNA_pol_II_repeat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007075">
        <property type="entry name" value="RNA_pol_Rpb1_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007073">
        <property type="entry name" value="RNA_pol_Rpb1_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038593">
        <property type="entry name" value="RNA_pol_Rpb1_7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006336">
        <property type="entry name" value="PRK08566.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF37">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04992">
        <property type="entry name" value="RNA_pol_Rpb1_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04990">
        <property type="entry name" value="RNA_pol_Rpb1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05001">
        <property type="entry name" value="RNA_pol_Rpb1_R"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00115">
        <property type="entry name" value="RNA_POL_II_REPEAT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 15" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="17">
        <location>
            <begin position="240"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1500"/>
            <end position="1748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1518"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1545"/>
            <end position="1730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1737"/>
            <end position="1748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW33664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1748" mass="193975" checksum="EFD8873CB44D1000" modified="2011-11-16" version="1">MSRQFPYSSAPLRSVKEVQFGLLSPEEIRAISVAKIEYPETMDQTTKKPREGGLNDPRLGSIDRNFRCQTCGEDMAECPGHFGHIDLAKPVFHIGFIAKIKKVCECVCMHCGKLLLDESNPAMAQAIKIRDPKRRFNAVWSVCKTKMVCETDVTEEDMQVDGKPNQQKPTTRGGCGHTQPTIRRDGLKLWGTWKHNKNFDENEQPERRLLSPSEILNVFKHINSEDCYKLGFNEDYARPEWMLITVLPVPPPPVRPSIAFNDTARGEDDLTFKLADVIKANINVQRLETDGSPQHVISEFEALLQFHVATYMDNDIAGQPQALQKTGRPIKSIRARLKGKEGRLRGNLMGKRVDFSARTVISGDPNLDLDQVGVPISIAKTLSYPEIVTPYNIHRLTEYVRNGPNEHPGAKYVIRDTGDRIDLRYNKRAGDIALQYGWKVERHLMDDDPVLFNRQPSLHKMSMMCHRVKVMPYSTFRLNLSVTSPYNADFDGDEMNLHVPQSPETRAELSQICAVPLQIISPQSNKPVMGIVQDTLCGIRKMTLRDTYIEFDQVMNMCYWIPNWDGVIPPPAIVKPKPLWTGKQLLSMAIPKGIHLQRFDGGRDLLSAKDTGMLIVDGEIMFGVVDKKTVGATGGGLIHTVMREKGPQVCAQLFSSIQKVVNYWLLHNGFSIGIGDTIADASTMKDITTTIQEAKTKVQEIILDAQQNRLEPEPGMTLRESFEHNVSRVLNQARDTAGRSAEMNLKDLNNVKQMVTSGSKGSFINISQMSACVGQQIVEGKRIPFGFADRSLPHFTKDDYSPESKGFVENSYLRGLTPQEFFFHAMAGREGLIDTAVKTAETGYIQRRLVKALEDIMVHYDGTTRNSLGDIIQFIYGEDGIDGTQVEKQSVDTIPGSDESFDQRYRIDVLDPAKSISESLLESGKEIKGDVKLQRVLDEEYHQLLDDRRYLRDVCFPNGDFSWPLPVNLRRIIQNAQQIFHNGRYKASDLRLDEIILGVRQLCTKLLVVRGDSPLSKEAQANATLLFQCLLRSRLAARRVIEEFKLNRASFEWVVGEIETQFQKSIVHPGEMVGVVAAQSIGEPATQMTLNTFHYAGVSSKNVTLGVPRLKEILNVAKNIKTPALTVYLDPEIAADIEKAKVVQSAIEHTTLKNVTSSTEIYYDPDPRTTVIEDDYDTVEAYFAIPDQKVEESIEKQSPWLLRLELDRAKMLDKQLTMAQVAEKISQNFGEDLFVIWSDDTADKLIIRCRVVRDPKSLDEDADAEEDQILKRIEAHMLESISLRGIKGITRVFMMQHKVSKPDETGEFKQGKEWVLETDGVNLADVMAVPGVDSSRTYSNDFIEILSVLGIEATRTALFKEILNVIAFDGSYVNYRHMALLVDVMTSRGHLMAITRHGINRADTGALMRCSFEETVEILLEAAASAELDDCRGISENVMLGQMAPLGTGSFDVMVDDKMLQFASSNIATEEYNDEGGATPYREYDMEDDKIQYEEGAGFSPIHTAPVSDGGAGGLTSYGGQPTSPSATSPFTYGATSPSYGGRASPGYGYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPQYSPTSPSYSPTSPSYSPTSPQYSPTSPSYSPTSPQYSPGSPEYSPNSPKKEKNDDEVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIS7</accession>
    <name>G3AIS7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Calcofluor white hypersensitive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_65626</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34492.1"/>
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    <sequence length="985" mass="110872" checksum="28898F4A5A5742ED" modified="2011-11-16" version="1">MSDTEQNQPVSVANEKSEIFRFNASIISYLHTTFAYSAFVVAVGVGCYLHYYKIVQNASYGYPDEWFPSVSATIGDRYPERSIFQILIALTAGPRFLLLFFNFLCLFKQNSYLPYVGLLSGTLRTFTAGGWMYITSTDDHDAHDVFMISYMVLTIPWTVCITLLSERNSFQRKARFYSATAFFSTIVPLIYWFIQHKVHIVAGAYSVYAYFEWGLIFLDILFDSWSAIDFSKVDIVLSGSAKKKPIEKPVVKTKSIGDEFTVFELLANLGNSFVFWSVSTSIFLCVWYFPLWHMGLSGYEAVITCYFLSPLLLILPFVRRFFTKFTYITRVLTVVLGVGAFKVPDPEQRLLVITAGTSFAVISLIIELGILADQPKKLHSYIITVLLGLLATSISKYLNFSNNPIWPIMHNENGGYNNIGLFLGLLAAFFTPGPSFKTEDSEKKKPVARTGGSILLASLGFGGYFFAIQSYLSDSGTLATWSWNGFPITGPTPITGGPLQFATIIIALFITAKYHPNVFSGLGYNLVVGGGSAVVLYLFEGWLGYAGSLVYSFYLVSIGPIIWQGIVGYNIAGAFFLAFFVDIIISLGSVWIVAYAFVPGGPLLRERTDIILFSSFVSILAGVANFNLRKGTIIKTTFISGTFLKQTATILTVCTAIVLAAFIKRYPTSAPVPYHADAQSFNAGIWCVHFGLDNDMWSSETRMRDLIRDAELDIIGLLESDTQRLIGGNRDFTQKIAEDLGMYVDYGPGPNKHTWGAALLSKFPIIESRHHLMPSPVGELAPAIFATLDIYGELVDVIVFHSGQEEDVEDRRLQTQGVTELMGNSTRPLVLLSYLVTEPLKGNYNTYVSEQSRMYDIDNTDWDRWCEYILFRGVKKVAYARISRSTITDTELQVAKFKLMTDEEKRDDDLGFYYGNNYINEEQVDENLRMPRMFRGEGVRGHRYHVFDEPRYFAQHKSQIVKDEPVEEAHEEQSQNHEERQNNED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIT0</accession>
    <name>G3AIT0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_134252</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
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        <property type="project" value="UniProtKB-SubCell"/>
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            <end position="17"/>
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            <dbReference type="RuleBase" id="RU363079"/>
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        </source>
    </evidence>
    <sequence length="659" mass="74465" checksum="BC6DF1E2ED323E5E" modified="2011-11-16" version="1">MWLLCLLLLPGFIYGHAFDVSFSPNYYKPGDKVDLLVNKVESDSTQLPYSYYSLPFVCPPMGDAHPVHLSLGEILRGDRIWSSSYDLKFGIDAPCSRLCDLRTSETGLKRADVLIKSGYVVQWLIDGLPGATTFESSNRNRKYYAAGFPLGFVKDDVSYIYNHFMLVIRYHRETNGKYTIVGFEVYPKSVSNEQCPGSSKNYKNFAIKYSADKDGNLIHQKTLIPYTYAVYWREDNSVDYDSRWDLYYENETNGAHVHIHWISFINSIILIFLGSLIVMIVLVKVLKKDIPPAGTKSPLPMHEFDTSAPDSTWRRLFDDVHRVPPATLLLTVLVSGGIQMLIASVGVVSIFVFDSFGGTRFTIFTNHRGQTFSLMIFCFIGSALVSSYSGIILYKVFNGDPLNQQYPRGRTIKLSLLFSGMLPMFIFIVVLLLNFFVWAKESSTALPFGTIMILILLFTLIQCPLGIIGAIYGNKRKFNTSKNSSLSTTPLDRPVSPEKIHTVSPAFSWIPAYITRTLTYGLIPFGIVFVELSFIFQSVWLEKTTFYYMYGFLLVTIIMLIIIIVESTIIAHYISLMIYQDSNWTWLSFQVGSSIGWYVFAYSIYYYFTVLNVGDFVSGVLYFGYMACACLIMGVTCGSIGVLTGLAFVKRVYGAIKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIV0</accession>
    <name>G3AIV0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_59123</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373345.1">
        <property type="nucleotide sequence ID" value="XM_007373283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIV0"/>
    <dbReference type="GeneID" id="18872784"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59123"/>
    <dbReference type="HOGENOM" id="CLU_2747168_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIV0"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="71" mass="7069" checksum="0BD94FE5193F0DA6" modified="2011-11-16" version="1" fragment="single">GGTGAGVGGRSAAGEAHAGIEDPTPVLIQPNNDFGDPVPVAVVDSGNVLLPSMSNLILLLVTVLHLIALLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIV2</accession>
    <name>G3AIV2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_134938</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373347.1">
        <property type="nucleotide sequence ID" value="XM_007373285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIV2"/>
    <dbReference type="FunCoup" id="G3AIV2">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIV2"/>
    <dbReference type="GeneID" id="18869854"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134938"/>
    <dbReference type="eggNOG" id="KOG0959">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008088_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIV2"/>
    <dbReference type="OMA" id="HLCEHMI"/>
    <dbReference type="OrthoDB" id="952271at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000012">
        <property type="entry name" value="Protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032632">
        <property type="entry name" value="Peptidase_M16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054734">
        <property type="entry name" value="PqqF-like_C_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16187">
        <property type="entry name" value="Peptidase_M16_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22456">
        <property type="entry name" value="PqqF-like_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="10">
        <location>
            <begin position="36"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="11">
        <location>
            <begin position="203"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 middle/third" evidence="12">
        <location>
            <begin position="400"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="Coenzyme PQQ synthesis protein F-like C-terminal lobe" evidence="13">
        <location>
            <begin position="816"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="992"/>
            <end position="1019"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF22456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW33763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1126" mass="129653" checksum="5763790BA836B2E5" modified="2011-11-16" version="1">MEYFSASTVNTQFKRPQANLSKSYKHILLNNGLSTLLISDPQEKISACSICVKCGTYNDPPDIPGLAHFCEHMVFMGSSEFPHPNEFFNCINTLGGECNAFTSGEKTCFYYEIPVNKSEINEELGFSYSLSVFSSFFKSPLFNKTSMSMEVDLINQEHELNISNEDKVLLHGMRLLANNNHPFHRFGTGNKKTLMSTKRSKVREEIIKFYQTYYFAENMILVLKGPQSINQLQKLAIANFRAIKKTPDEPEQSRISHPVTNYKIHEVFSNWNQVLYIQHSKWTKVRLLLPIPSSTSKFLENVWCNLLGDESCGTICEYLKHEKGFLNSIYVFSHKFTAKQKALAIDLGLTKSGETNLSTVVSAIFNFIAQILSQSDETLHQLLSEYSRVFKFNAYFQTNQDSPMIEVSNLALLLQDRELPLEDIILGSSLTIKKDVGKFRKRTIELFNIDKLNIIILSDKPQLVEQVTLPATGKLEKDPFYNFGYKTYIFKPPRRGDIFPEFSLILENRFIPFTCEQLDRAIANSKLKKFQFMGEIPQDQTPKLLDYSNYHEIWISESRNLTNIVFTSFQIRFSQVKFTPVNCIGVEIMAILFETFLLQKLYLAELANYSWAIYPNYNIAPSFTFAACGPKHGFKYFLHEFVKMINGFLNNGVNNLGYKRFTRAKLELRDEYEKLLSRDAMKQALAISILALESGICPLNERMEALEIISIEDLQRICKIIKKDQSQVQVLISGNIDRKFIMDVSTIINTLSHHQQTILDGVNFPQPKSVRLKVGANYAYVHNNPDEKVRADDVYHYIQLGRRDDSSVRTMAAFLSYLLSQTVTYELRTKKQVGYMVLSGVRYNKETLGIHIYVSSSKFTYSEVMSEIEQLLFDWEIRLLNYTQDDLDKQINTFLEGCDRFPEEQLPSNILYGVPPSQSSGNFSTDDKGFKRHQCYWEKILTGNFDFSVGNMKEELDIDLLEGMKTESLLTFFGTYISIKSKVRSTLSIFISSRKARELQEYREAKETLQELLSAKQYNLSPIQIKMLVREHNRDISQIVKHLQARGYDIKQPKTNLSLLKLNNSSNNEKEFSKLQQKCIEKFGKLYKDKSRVVPLIEVSKILEIHAGSSICPTKDRYSSIKKHAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJA4</accession>
    <name>G3AJA4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL3m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_59991</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374147.1">
        <property type="nucleotide sequence ID" value="XM_007374085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJA4"/>
    <dbReference type="FunCoup" id="G3AJA4">
        <property type="interactions" value="869"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJA4"/>
    <dbReference type="GeneID" id="18873168"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59991"/>
    <dbReference type="eggNOG" id="KOG3141">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044142_4_1_1"/>
    <dbReference type="InParanoid" id="G3AJA4"/>
    <dbReference type="OMA" id="GKNIPCT"/>
    <dbReference type="OrthoDB" id="274683at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000004">
        <property type="entry name" value="50S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019927">
        <property type="entry name" value="Ribosomal_uL3_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_uL3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03625">
        <property type="entry name" value="L3_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11229">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11229:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL3M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="279" mass="30277" checksum="FC4F44C761068BFC" modified="2011-11-16" version="1">MLSFNSVSSRVLQTLNSTFNFTATRHACKITSIHAITSMKTQPNQVNHSVELGNRRKCLLDRPGLFGIKRGMITWFNDNGRQFPATVIEIDSVEVIKHKTEQEFGYTSVMVGAVDKLKNVNNLKLFEQAGVSPKHKLGEFRVRSETGLVPVGAELKADMFAVGQMVDIKGITKGKGFAGVMKRHGFKGLAATHGVSKAHRKQGATGGNQDPGRVLPGKKMAGRMGGRSSTEMNLEVLHADGDAGILVVKGCIPGPNKAYVKITDSVKLYGKSIESIERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJD6</accession>
    <name>G3AJD6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_60031</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374179.1">
        <property type="nucleotide sequence ID" value="XM_007374117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJD6"/>
    <dbReference type="STRING" id="619300.G3AJD6"/>
    <dbReference type="GeneID" id="18873186"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60031"/>
    <dbReference type="eggNOG" id="KOG2519">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_999497_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJD6"/>
    <dbReference type="OrthoDB" id="2959108at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="279" mass="32200" checksum="96E5C071CC32C7EA" modified="2011-11-16" version="1" fragment="single">MDNTQYYVTPVDINKGEESGEFTLEKLIFLASLRGGDYSNGVGRIGIKYAILLALPSTSENATELKQELANELQKVEGDDLEEVPEEKPVPNFADEFVDCFTDRIRTSLIRSWDMRLAEDARKRRLREFSHRLSLYIRDHARKVFKRRFNLKKDIVIDEYATMHYMFPFINTNVFLFQPDSLNCCELEADCSSIQVPNNFLFQKFEAKSEENIIRENGNKTFDQVLCFKDGKIKQVITVSQTHPAVTHFPVPRSFTGNVKHVIFRLATDKSVPITITKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJK2</accession>
    <name>G3AJK2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_54170</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373489.1">
        <property type="nucleotide sequence ID" value="XM_007373427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJK2"/>
    <dbReference type="FunCoup" id="G3AJK2">
        <property type="interactions" value="903"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJK2"/>
    <dbReference type="GeneID" id="18871840"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54170"/>
    <dbReference type="eggNOG" id="KOG0789">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001645_9_12_1"/>
    <dbReference type="InParanoid" id="G3AJK2"/>
    <dbReference type="OMA" id="WSIDKAP"/>
    <dbReference type="OrthoDB" id="10253954at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18533">
        <property type="entry name" value="PTP_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000115">
        <property type="entry name" value="Tyrosine-protein phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016277">
        <property type="entry name" value="Ptp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF449">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000938">
        <property type="entry name" value="PTPN1_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="7">
        <location>
            <begin position="3"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="8">
        <location>
            <begin position="211"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="316" mass="36993" checksum="E51520158BD75BC8" modified="2011-11-16" version="1">MNQREKFNELNELEADRIQDGLDNPDTSIWSLTHAMKRVNRGRNRYSNVFPWNETRVVLPVKDDKEFSDYINASRIKLSLSDEGAFDEYIACQGPLESTYNHFWSMCFNESEVQNNDTVVIVMVTPLTENGVVKCARYWPELNQTWDFSTDNIEDGINVPNLVITNKSETFDSNEDFIITELELKSNDKIKTVYHYYYFKWVDAKVPPSIEPLFKISQDISSIRSTMETPPVPIVHCSAGVGRSGTFIAFDHLFGRNLDEFIDLVRKKQPYQYDIVYKTVYQLRSQRMMMVQTIHQYNFLYEVAKMVFNENVNRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJP5</accession>
    <name>G3AJP5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">dihydrolipoyllysine-residue succinyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">2-oxoglutarate dehydrogenase complex component E2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_59334</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.61" evidence="5"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373530.1">
        <property type="nucleotide sequence ID" value="XM_007373468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJP5"/>
    <dbReference type="FunCoup" id="G3AJP5">
        <property type="interactions" value="884"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJP5"/>
    <dbReference type="GeneID" id="18872873"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59334"/>
    <dbReference type="eggNOG" id="KOG0559">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJP5"/>
    <dbReference type="OMA" id="NMPQTAV"/>
    <dbReference type="OrthoDB" id="5391403at2759"/>
    <dbReference type="UniPathway" id="UPA00868">
        <property type="reaction ID" value="UER00840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004149">
        <property type="term" value="F:dihydrolipoyllysine-residue succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033512">
        <property type="term" value="P:L-lysine catabolic process to acetyl-CoA via saccharopine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000006">
        <property type="entry name" value="Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050537">
        <property type="entry name" value="2-oxoacid_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006255">
        <property type="entry name" value="SucB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01347">
        <property type="entry name" value="sucB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416:SF5">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="14">
        <location>
            <begin position="55"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="135"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="177"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW33946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="436" mass="46739" checksum="33169F2F5355C7D3" modified="2011-11-16" version="1" fragment="single">MLSRTIKSSLKRVPRPLAVSGLKVTNAVAIRSMHIQSSNRAFDRPMNLSFSRFASTTVKVPDMAESITEGTLTAFNKEVGEYVNQDEQIATIETDKIDVEVNAPVAGTITEFLVELESTVEVGQALATIEEGAAPEGGAAAAAPAAPKEEAKPAESAAPAASAAAPAATPAAAPAAPKKEAPAPKKEEPKAAEAKSGATFTNFSRNEERVKMNRMRLRIAERLKESQNTAASLTTFNEVDMSNLMEMRKLYKDEFLEKTGTKLGFMGAFAKASCLAAKEIPAVNAAIENNDTLVFRDYTDISIAVATPKGLVTPVLRNAESLSILGIEREISNLGKKARDGKLTIEDMTGGTFTISNGGVFGSLYGTPIINMPQTAVLGLHGVKQRPVTVNGQIVSRPMMYLALTYDHRVLDGREAVTFLKTVKELIEDPRKMLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJS7</accession>
    <name>G3AJS7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_134326</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373562.1">
        <property type="nucleotide sequence ID" value="XM_007373500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJS7"/>
    <dbReference type="FunCoup" id="G3AJS7">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJS7"/>
    <dbReference type="GeneID" id="18869803"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134326"/>
    <dbReference type="eggNOG" id="ENOG502QQWB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008201_1_0_1"/>
    <dbReference type="InParanoid" id="G3AJS7"/>
    <dbReference type="OMA" id="RKTWIYE"/>
    <dbReference type="OrthoDB" id="2155291at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000935">
        <property type="term" value="C:division septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035838">
        <property type="term" value="C:growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030950">
        <property type="term" value="P:establishment or maintenance of actin cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04399">
        <property type="entry name" value="RhoGAP_fRGD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF17">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN RGD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-BAR" evidence="5">
        <location>
            <begin position="1"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="DEP" evidence="3">
        <location>
            <begin position="215"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="479"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="688"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="691"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW33978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="801" mass="91826" checksum="F7EEA93F3C1F0A21" modified="2011-11-16" version="1">MSFADSFWTSDYSSGFKVLFEQLHQGICENEDFISLFHKRMESELLYGTQLESIEKTAIKSTKNKRQLNDDYVSTIKNAYSKMNENFTKQGQYHLEIANNIKTLVLAPFSKWCKEHQGRVEYSESMISEKSKLFKSKKAHLDKLQKRYFNKCRMLEECKSHYTEEELREELDELTFQQKLEDAYEEEVPDDEEERYYFGNITYDHKQMKALLTDMLTNIPLTSHKVAILGTYHNVSTGSSIIQWLIDNLPQFNRNLEKAEKFASELINNGFIRLIGTMTSGKGFINSSQFYYQWKPLAFEITKLSQLQISSSTEDTNNANGTVPKIEGTGGAKIQEYFDDMKEAIGVGAVDYTDPAQLPKLIKEVNQLDQQYYASVVDLDKTRCEFEELIMDHLTFMQKCELDRLKAIKKVMFDFLASFSNGMDELKNFYDELLLLEETIHPINDLKFLIENYATGGFKPCVVLYDNYYDSHVKQTFGVDLNIKSRLDKKVVPILVQCILSYLDSVYPDLENDEERINLWTQPIHLAQVHKLRSELNDNNSDPQYINSILEQNHPLVITNVLKLYFMELPDSIVPRTNYDVIKLLYLNYPPNDESKTSSRINGLQNILCDLPKCNIATLDALLTHIARLINIIGSKDETLAVTLQSSLAKEFAPLVLRQKEQSELNDKHQVNFITDLLNHKETIFTELRRQSSTRPANSASRDNSAHVSRAGSAHKALTPKESVAATSRSRLEQRLQTVVRKSKKETKEDSSSDSAPETPTKVGSLKRSSSPKKRLSAILVDEDVARSLSPSKAPDTPIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJY9</accession>
    <name>G3AJY9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Glucose-6-phosphate isomerase</fullName>
            <ecNumber evidence="3 10">5.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_59465</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the GPI family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="3 10"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373624.1">
        <property type="nucleotide sequence ID" value="XM_007373562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJY9"/>
    <dbReference type="FunCoup" id="G3AJY9">
        <property type="interactions" value="1171"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJY9"/>
    <dbReference type="GeneID" id="18872927"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59465"/>
    <dbReference type="eggNOG" id="KOG2446">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017947_3_1_1"/>
    <dbReference type="InParanoid" id="G3AJY9"/>
    <dbReference type="OMA" id="DWYRQLW"/>
    <dbReference type="OrthoDB" id="5831190at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047938">
        <property type="term" value="F:glucose-6-phosphate 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05016">
        <property type="entry name" value="SIS_PGI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1390.10:FF:000001">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000004">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00473">
        <property type="entry name" value="G6P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023096">
        <property type="entry name" value="G6P_Isomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018189">
        <property type="entry name" value="Phosphoglucose_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035482">
        <property type="entry name" value="SIS_PGI_2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001211">
        <property type="entry name" value="PRK00179.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469:SF1">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00765">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="6 10" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 10 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="550" mass="61143" checksum="B9691F6C1BD01C1F" modified="2011-11-16" version="1">MSSFKLASELPAWKKLQATYDSVGEKFSVKDEFSKDSSRFETFSRTFENYDGSKILFDFSKNLVNKEILDQLIELAKEAGVEKLRDEMFAGDHINTTEDRAVYHVALRNRALKKMPVDGKDTAKEVDDVLQHMKEFSDEVRSGKWTGYTGKAITDVVNVGIGGSDLGPVMVTEALKAYAQPGLNVHFVSNIDGTHIAETLKNLNAETTLFLIASKTFTTAETITNANSAKSWFLEHAKDKAHIAKHFAALSTNEKEVAKFGIDTKNMFEFNDWVGGRYSVWSAIGLSVAIYIGYDNFEAFLKGAEAVDQHFLTTPLEQNIPVLGGLLSVWYNNFYGAQTHLVAPFDQYLHRFPAYLQQLSMESNGKSVTRANVYTNYQTGTILFGEPATNAQHSFFQLVHQGTKLIPADFILAAQSHNPIENNLHQRMLASNFFAQAEALMVGKSTEQVKAEGATGGLVPHKEFSGNRPTTSILAQKITPANLGALIAYYEHVTFTEGAVWNINSFDQWGVELGKVLAKVIGKELDDKTKVETHDASTRGLINQFKEWSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJZ1</accession>
    <name>G3AJZ1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59466</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373626.1">
        <property type="nucleotide sequence ID" value="XM_007373564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJZ1"/>
    <dbReference type="GeneID" id="18872928"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59466"/>
    <dbReference type="HOGENOM" id="CLU_2801140_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJZ1"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="68" mass="6814" checksum="BBEFCEB66C01BF17" modified="2011-11-16" version="1" fragment="single">STNVGNSGSSSSKPNQSQMNPGSGNAGNTLSMSQSPTTSSINSVIGYDNGATSWNAPVALIILINCII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK60</accession>
    <name>G3AK60_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Plectin/eS10 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60213</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374386.1">
        <property type="nucleotide sequence ID" value="XM_007374324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK60"/>
    <dbReference type="FunCoup" id="G3AK60">
        <property type="interactions" value="979"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK60"/>
    <dbReference type="GeneID" id="18873276"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60213"/>
    <dbReference type="eggNOG" id="KOG3344">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089349_4_1_1"/>
    <dbReference type="InParanoid" id="G3AK60"/>
    <dbReference type="OMA" id="ERTKIHR"/>
    <dbReference type="OrthoDB" id="5211809at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000025">
        <property type="entry name" value="40S ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005326">
        <property type="entry name" value="Plectin_eS10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037447">
        <property type="entry name" value="Ribosomal_eS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12146">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12146:SF0">
        <property type="entry name" value="RIBOSOMAL PROTEIN S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03501">
        <property type="entry name" value="S10_plectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Plectin/eS10 N-terminal" evidence="7">
        <location>
            <begin position="3"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="102"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="113" mass="13400" checksum="BBB286E17A8BBE8A" modified="2011-11-16" version="1">MLIPREERKKIHQYLFQEGVVVAKKDFNQPKHEEIDTKNLYVIKALQSLTSKGFVKTQFSWQYYYYTLTDEGVEFLRTELNIPEGILPLTRLKNAPAERPRAGPRRGFRRSRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK85</accession>
    <name>G3AK85_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nitrogen regulatory protein areA GATA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_55408</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375059.1">
        <property type="nucleotide sequence ID" value="XM_007374997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK85"/>
    <dbReference type="FunCoup" id="G3AK85">
        <property type="interactions" value="358"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK85"/>
    <dbReference type="GeneID" id="18871934"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55408"/>
    <dbReference type="eggNOG" id="ENOG502QSII">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014358_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK85"/>
    <dbReference type="OMA" id="WKYIILK"/>
    <dbReference type="OrthoDB" id="5563539at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007039">
        <property type="term" value="P:protein catabolic process in the vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013860">
        <property type="entry name" value="AreA_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052292">
        <property type="entry name" value="Glucose_repression_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28051">
        <property type="entry name" value="PROTEIN MTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28051:SF1">
        <property type="entry name" value="PROTEIN MTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08550">
        <property type="entry name" value="GATA_AreA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitrogen regulatory protein areA GATA-like" evidence="3">
        <location>
            <begin position="187"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="507"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="572"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="750"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="308"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="508"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="759"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="784"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="819"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="847"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="862"/>
            <end position="879"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="926" mass="102344" checksum="884A1A5DBE1B8A9D" modified="2011-11-16" version="1">MSISGEVYSGGATTSQHIEAEDEEHFQNTTFKLKRTRSIGVLDEFIPDKLADGNQNNEPPPPTQLSPPVLNNINSRPSSNASSTSSSSSSTSSPFEEDSINTAPTATTISNNVAVPEQPPAIDTSVASNAANGSVSVSSPVSHSPSPLPLLQSPELLPHDDTDLMIEPSRHVDYLSYQWDVNDISKSWRYVISKKHNVANSKRLENASWRTWAQRRQNLKTISPEVVNWSKDSDVTWLYGPILNDDHASDDETCNIKRETTATSAVAGDISIANKKKPSAPKPILKKRTVQESMISHSDLLKLQLATNKVYQKQREQLLKQQQEIKQQEEQQKKVPAAPEEKFFDYDTLSAKLNKQYENTAIPENCNVIKLQDLLNSRSNSKTKLAESANVVQASENVVEPMSKLTLASFEKSPEVVPQEEEVEEEEEEVPVKEDRHIHFNEEVQQCIAVNPYSDNDAIAEEEEDEEDDDEDYYDYNDYEDEEIGNDGYYDNVANYYYDGSKSHLYDPEDYSDVEDDDDDDEGGFFIKVKSNSNAPLILARNATNENTEDSESISTSNSKVYKTIHLLPSTSLNYGSSDEESDDENPYTSSLSHNVNNEISRGYDYYYDYNTVYTVDPNHAIYGKTPDVVDVPDNLGVGSNFDYDIIENDEMPPMVINMTSPVMYASQPNSPLAVANAPSIPQVAPSPVLLATKAPFHMSDDEDSDSDDGLSIGTRRSSQALAESIFHTTPSNPQHFPEKFLKDPEPVEAPITTINPRHSSTSISKQPTSSHSLSQSFFGAGGAPTAMSTTTAETEALSHSFFEKRTPSPVVLSSTPDTTSNSLTRTLSNKSSPLPPQTTSETALFKSSSSGTSQTKGMFSFDDDDTDEENDEHDEEHDGSDLFIGKTYNSLSQVADKSGIRSPSPMDENKNLMGQAKGLAKHFFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKD2</accession>
    <name>G3AKD2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Stress protein DDR48</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60232</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374404.1">
        <property type="nucleotide sequence ID" value="XM_007374342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKD2"/>
    <dbReference type="STRING" id="619300.G3AKD2"/>
    <dbReference type="GeneID" id="18873288"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60232"/>
    <dbReference type="eggNOG" id="ENOG502QUR8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1759932_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKD2"/>
    <dbReference type="OMA" id="DSMVDQK"/>
    <dbReference type="OrthoDB" id="4026468at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="16172" checksum="83ED23D1B04DDFA2" modified="2011-11-16" version="1">MVFDFGKDDDDKNKDYSSSGRRNDLDDSNIDSFGSSNDDSYGSSRKGDSYGSSNRDDSYGSSNRDDSYGSSNRDDSYGSSNLGSNLGSKLDSSIDSARDTFGSSRGKDSYGDDNDFSGSRQQGGNQDYGNNDDSWQSSGNRRRDDDNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKD4</accession>
    <name>G3AKD4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_135727</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374406.1">
        <property type="nucleotide sequence ID" value="XM_007374344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKD4"/>
    <dbReference type="FunCoup" id="G3AKD4">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKD4"/>
    <dbReference type="GeneID" id="18869913"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135727"/>
    <dbReference type="eggNOG" id="KOG0036">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0751">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_2_2_1"/>
    <dbReference type="InParanoid" id="G3AKD4"/>
    <dbReference type="OMA" id="SGQWWKQ"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="418"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="465"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="33"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="71"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="111"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="139"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="205"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="358"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="466"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW32891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="562" mass="62367" checksum="14132D0038B314E8" modified="2011-11-16" version="1">MTGLATSSTPGYSPSDSPKNESSKSSSIPTDTTKLNDYEALFKTLDIEDTGEITLRDFKRAIKGLNHPISSSPELMKKIFDSFDANQDRVIDFNDFKVYLSTTDKQILRGFNILDEDHDGKLNKADFVRYLKESLKLDPTTADIDLLFKKIDYKNDGYITYDEFRQFLLLMPRLNGSRIKTAYHFIVEEFDITSDGDVALVTQFLNGFGYFLAGGLSGVVSRTCTAPFDRIKVFLIARTDLTSTVLHSKQEIATQIAAGAERHIIDELRKKLAHAELQVAQEKAAAEAASSLREKTIRSPIVQAARTIWKQGGFKAFYVGNGLNVLKVFPESAMKFGSFEAAKRFFAGIEGVDDSSKISKVSTYLAGGFGGVVAQFTVYPVDTLKFRLQCSNLDSSLKGNALLIQTAKDMFQEGGLRIFYRGIFVGTSGIFPYAALDLGTFSIIKNWLVKRQSKKTGIRQEDVKLPNYMVLTLGALSGSFGATLVYPINLLRTRLQAQGTYAHPYTYNGFFDVLNKTVAREGIPGLYKGLVPNLAKVAPAVSISYFMYENLKSLFNLNSTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKI3</accession>
    <name>G3AKI3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_60280</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">GTPase involved in activation of the TORC1 signaling pathway, which promotes growth and represses autophagy in nutrient-rich conditions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the GSE complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GTR/RAG GTP-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374455.1">
        <property type="nucleotide sequence ID" value="XM_007374393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKI3"/>
    <dbReference type="FunCoup" id="G3AKI3">
        <property type="interactions" value="257"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKI3"/>
    <dbReference type="GeneID" id="18873315"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60280"/>
    <dbReference type="eggNOG" id="KOG3886">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044099_1_0_1"/>
    <dbReference type="InParanoid" id="G3AKI3"/>
    <dbReference type="OMA" id="LIPNMQD"/>
    <dbReference type="OrthoDB" id="10020193at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071986">
        <property type="term" value="C:Ragulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903778">
        <property type="term" value="P:protein localization to vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11384">
        <property type="entry name" value="RagA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000488">
        <property type="entry name" value="Small monomeric GTPase (Gtr1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039397">
        <property type="entry name" value="RagA/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF1">
        <property type="entry name" value="RAS-RELATED GTP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04670">
        <property type="entry name" value="Gtr1_RagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="322" mass="37243" checksum="D30640275EF8FBE5" modified="2011-11-16" version="1">MSHASRKKLLLMGRSGSGKSSMRSIIFSNYSAFDTRRLGATIDVELSHLRFLGNMTLNLWDCGGQDVFMDNYFTNQKDHIFKMVQVLIHVFDVESKSINKDIEIFIKSLTNLQKYSPGAKIFVLLHKMDLVQIDKRQELFTIMMEKLQKISNPYQFKLIGFPTSIWDESLYKAWSQIVCSLIPNINLFNSNLIKFNSILDAEEIILFEKTTFLVISSTASMNQAIEGADEEHEETTTKEEDLDPKRFEKISNIIKTYKQSISKLRTNFNNLIIRGSNGNNFYIDILTDNMFIMIVLKDTVEELLILDNIKAARKWFEKIENA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALG9</accession>
    <name>G3ALG9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="2">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_137293</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="2"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374727.1">
        <property type="nucleotide sequence ID" value="XM_007374665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALG9"/>
    <dbReference type="FunCoup" id="G3ALG9">
        <property type="interactions" value="160"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALG9"/>
    <dbReference type="GeneID" id="18870035"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137293"/>
    <dbReference type="eggNOG" id="KOG0198">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003051_0_1_1"/>
    <dbReference type="InParanoid" id="G3ALG9"/>
    <dbReference type="OMA" id="CFNADSI"/>
    <dbReference type="OrthoDB" id="266718at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032093">
        <property type="term" value="F:SAM domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000196">
        <property type="term" value="P:cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007232">
        <property type="term" value="P:osmosensory signaling pathway via Sho1 osmosensor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038066">
        <property type="term" value="P:p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071507">
        <property type="term" value="P:pheromone response MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001402">
        <property type="term" value="P:signal transduction involved in filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09534">
        <property type="entry name" value="SAM_Ste11_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000334">
        <property type="entry name" value="Serine/threonine-protein kinase STE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000387">
        <property type="entry name" value="Serine/threonine-protein kinase STE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029458">
        <property type="entry name" value="Ras-bd_By2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584:SF369">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14847">
        <property type="entry name" value="Ras_bdg_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01304">
        <property type="entry name" value="Ras_bdg_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM" evidence="12">
        <location>
            <begin position="17"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="450"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="479"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW33212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="726" mass="81743" checksum="7996A394629225CF" modified="2011-11-16" version="1">MSDTTEIVEITQADPRNDVKELKQWLTNSDCQQYFPKFVDNGITLDLVPELDTEALKELGITKLGDRLRLELAISSMKQEQLQQRVGIEEILEMVNNKPAVVTPGTVAEANEATLTQLEGGNTTSITPSSKDSSKNIMFILQDGSMIKVNVGGCFNAQSIKRKVLKKLGFKSQEAQFDTYIHSTNSDGVSNVIMLFDVELVTICYSPERLEKHRIMFVPKNDYPTEVAIETSKKIMHKYASRSRDSSMNSRNQRSQMRNFFGQRPPSELISSNLAEYFPEARKKDLEQTVRNSVRHSVRLSRRFNIPPGAFSSGFNKRNSVWSNATGLASHKAVSISSGETGNGYRRSQRTIGDVMMNNISAIDEAVSSTETFHTPPTNTIFNDTKSIASSKKSRNALHRISMVADSLPAANRLSTIELISSDSEEEDIDGDDEFDKVDIFADFDVPKHWLKGARIGSGSFGTVYLGMNPHTGELMAVKQIPLPTEKNRHNTDVERSMAEQQREMTLLKELDHENIVRYFGSTTDENFLNIFLEYVPGGSVQTMLNSYGPFEEPLIRNFIRQVLIGLNYLHGEDIIHRDIKGANILIDIKGTVKIGDFGISRKVSSIDEEDEVFKKTGRRASLQGSVFWMAPEVVKQTVYTKKADIWSVGCLIVEMFTGRHPFPDLSQMQALFRIGSHIPPQIPEWCTQEAKDFLTETFELNYEKRPDAIELLAETFLNPLIMSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALL7</accession>
    <name>G3ALL7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_60600</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374775.1">
        <property type="nucleotide sequence ID" value="XM_007374713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALL7"/>
    <dbReference type="FunCoup" id="G3ALL7">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALL7"/>
    <dbReference type="GeneID" id="18873484"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60600"/>
    <dbReference type="eggNOG" id="KOG3780">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018982_1_0_1"/>
    <dbReference type="InParanoid" id="G3ALL7"/>
    <dbReference type="OMA" id="FINEPRS"/>
    <dbReference type="OrthoDB" id="2333384at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF17">
        <property type="entry name" value="FI21816P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="77"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="485"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="572" mass="62106" checksum="90B7A982712C2695" modified="2011-11-16" version="1">MLTPGNYEIPFSAILPGNIPESIEGLPGCSSIYRLEATIDRGKFHSSIVAKKRVRVIRTLTTDAVELSETMAVDNTWPQKVEYSLSCPMKAIAIGSGTPVSVMIVPLLKGLQLGDIKIQLVEMYSYVGYFPPVHSGERIVCQKKVPKPNPDALDLDKWEVETFLKVPASLSKCTQDVDLLTHVKVKHKLKFNIALINPDGHTSELRASLPVQLFISPFVSIRSIPDEETSSEDESQEVLFANSESQLSLASLHSNSSSHTSLTGLVAPPLYEQHIYDRLWSDVSPIETPVTSGATTPRAIRPAASSGSSSGGSDLHQFSMSPLDSNLLTENLRLLSLQRQSQEDNTFNGPSSPPNNASSSPLRIPAPTHTRDRATFNLDGDDYFARRPPARSPLHQSYINDHPSPIMSPPVHLSRVPSEANMSTNVSAEDLLQIPSYTQAIKGDITDDLAPAYEPPLPGSNIDLASANKRFEDLHKRTPISMTPTSSSLRASGRSSAGTSPSPSQSRGSSLVNLAGYFSHARSNGGSSTNLASLNEHTRSDNVGSKMSNSGMADRATLVNFHNMSFRKKDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALN8</accession>
    <name>G3ALN8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Globin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_50180</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374796.1">
        <property type="nucleotide sequence ID" value="XM_007374734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALN8"/>
    <dbReference type="STRING" id="619300.G3ALN8"/>
    <dbReference type="GeneID" id="18871413"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50180"/>
    <dbReference type="eggNOG" id="ENOG502RS13">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_347492_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALN8"/>
    <dbReference type="OMA" id="NVKKVPY"/>
    <dbReference type="OrthoDB" id="4025615at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008941">
        <property type="term" value="F:nitric oxide dioxygenase NAD(P)H activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071500">
        <property type="term" value="P:cellular response to nitrosative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046210">
        <property type="term" value="P:nitric oxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01040">
        <property type="entry name" value="Mb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044399">
        <property type="entry name" value="Mb-like_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396:SF6">
        <property type="entry name" value="ABL201WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396">
        <property type="entry name" value="FLAVOHEMOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Globin" evidence="2">
        <location>
            <begin position="4"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="488"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="657"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="488"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="657"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="694"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="717"/>
            <end position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="781" mass="88545" checksum="399AC4685AFC95BE" modified="2011-11-16" version="1">MDLYLSQVELERIQTSWSDIPSKRRFCNMLYSNLIIQNPHINQVFNNDDDLIDEHADTFKELFDYVIIHLRNMALVEEFITKFVNEHSSFCSLADKYLDTMGNCLLDTHKQILGDGFDKDMELAWIRVYVFIANYILKLTEEPSDAESASSLTDIEPLKIGKSPMTPNTPLTPIMDEDAAFEENTQDSENAETINTIKFLLSSNSKYRGFRRSSNIPKDEPITVSVPPSRRSTLSPGLRNSLSKKVETVCEVDENFDPRNRRRLSNATSFVTVKSQSPPSSIGDDKDETFDVVDSLSGDSSDEYEERNEREDEFSTPKPSFTSDRFRSPLLQRLREKQQWNAIDEDESDEEREQEEHQRKVHQQREEQKQRQWEHERQQMELARQMERLHPVEEEKPRLNLRESFPTIEQDDEPFDPRRRIRSRFNQPVKLSLRELTSSEEDLSTPFQQPGVPQRSPARLMKHAASTECFGLKGLAPIVEHEDKFIKSDTSSSRTSSLSLGASHSHSGSSMSSTTEDNSYSKPVAQSRNQQSSLCNLRYASSTPSLISEPNTPNRASLGFMRSSFVLKKEMDQLGYNIPENVSGNSGDILMAPVKLEAPRPQVHLLANDSMYSFEPPVKPDNSDDVSSINPFEIATANTSMSSEKSSRTSLRKKLGSLFSSKSSSQPAKRSTSKTTVKSSGASVITVDPKHYSSRNTSGQTSMFTARQMPEPPRNPFTPSVTSRSDCRSVASTETRSSTISGFSLLKKSDKRGVKYTLPEARHTSRGNRYVVTKVPYNIFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALT2</accession>
    <name>G3ALT2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60049</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374206.1">
        <property type="nucleotide sequence ID" value="XM_007374144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALT2"/>
    <dbReference type="STRING" id="619300.G3ALT2"/>
    <dbReference type="GeneID" id="18873197"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60049"/>
    <dbReference type="eggNOG" id="KOG1677">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_949941_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALT2"/>
    <dbReference type="OMA" id="FMKMGIC"/>
    <dbReference type="OrthoDB" id="410307at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000018">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="288"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="326"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="288"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="326"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="27"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="44"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="353" mass="39214" checksum="D5CE08F8107E7A77" modified="2011-11-16" version="1">MLSTSSMYGASNIGSGNSRNSPPPIGLQQQHKQPQNTPLKQHNSVSSSLTTSSASSSTSDFGDLHGDLLADLWHSSTNESMTSNGAINVKQPAAQYPSAIVPSVSQQPQQQQFFPQQQQQQPRLFNPNPVYTTQQVNWNTSNVSISPPILSSISQQQISTVAQPNSSSSPSLTVDPFIDSLHGYESSLLPRLTEENLMMQTKSDSFTTTHNPNVQFNIANDSEDLFNFEHHQQQQQQSRFVRSTPMLNGNVRQQPFVGHSHNSQHKESSDVHAHMKTNVSSATVNTQLYKTELCASFMKMGICPYGNKCQFAHGENELKVVERPPKWRSKPCVNWAKYGSCRYGNRCCFKHGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALX7</accession>
    <name>G3ALX7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Casein kinase II subunit alpha</fullName>
            <shortName evidence="11">CK II alpha</shortName>
            <ecNumber evidence="11">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_60665</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Phosphorylates YTA7 during S-phase to promote transcription of histones.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="11"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374846.1">
        <property type="nucleotide sequence ID" value="XM_007374784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALX7"/>
    <dbReference type="FunCoup" id="G3ALX7">
        <property type="interactions" value="806"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALX7"/>
    <dbReference type="GeneID" id="18873514"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60665"/>
    <dbReference type="eggNOG" id="KOG0668">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_70_4_1"/>
    <dbReference type="InParanoid" id="G3ALX7"/>
    <dbReference type="OMA" id="ACEKRPQ"/>
    <dbReference type="OrthoDB" id="10254671at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007535">
        <property type="term" value="P:donor selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006356">
        <property type="term" value="P:regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006359">
        <property type="term" value="P:regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14132">
        <property type="entry name" value="STKc_CK2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000459">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000088">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045216">
        <property type="entry name" value="CK2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054:SF27">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="13" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 11 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="40"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW33331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="327" mass="38240" checksum="6CB0E6F1A3612221" modified="2011-11-16" version="1">MISTRVYSTARVYADVNANKPQEYWDYENHKIHWGDIRNYEIISKIGRGKYSEVFQGVNVLNDEPCVIKVLKPVKLKKIYREVKILQNLTGGPNVIGLLDIVRDEQSKIPALIFERISNVDFRVLYPKFTIKDIQYYFTQLLIALDYSHSMGIIHRDVKPQNIMIDPLSKKLRLIDWGLAEFYHSGMDYNVRVASRYHKGPELLINLQQYDYSLDLWSVGCMLGAIIFKKEPLFRGDSNNDQLVQIAKVLGTENLMKYVQKYGLKLSSDYDDILGNYPRKPWKSFINNENRHLISEEVLDLIDKLLTYDHQLRPTAKEAMDHPFFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM27</accession>
    <name>G3AM27_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_65776</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374247.1">
        <property type="nucleotide sequence ID" value="XM_007374185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM27"/>
    <dbReference type="STRING" id="619300.G3AM27"/>
    <dbReference type="GeneID" id="18875061"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65776"/>
    <dbReference type="eggNOG" id="ENOG502S4TA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1057665_0_0_1"/>
    <dbReference type="InParanoid" id="G3AM27"/>
    <dbReference type="OMA" id="MPKNTLY"/>
    <dbReference type="OrthoDB" id="5970at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061574">
        <property type="term" value="C:ASAP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15481:SF0">
        <property type="entry name" value="LD23870P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15481">
        <property type="entry name" value="RIBONUCLEIC ACID BINDING PROTEIN S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="4"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="148"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="156"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="195"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="237"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="248" mass="27514" checksum="1B30DAC57CA49C5A" modified="2011-11-16" version="1">MPKNTLYVTGFSRESKAADLAPDFEKFGELVRLDIPPPRTDDGQKYAFVEYKDAESCEKALELDGKTLPYSLKDGLVVQIARSDPYSVRSRGGFRGGRGGYGYGGAPRGGYRGGYDRGYGYDDDRYAHRGPPRGGFRGDNGYRGGYGGFRGEDRGYGGGRGGYGGYRGDDRSYRGGRGGYGGDRDFRTRGPTYDNRGDRYRDEREGRGEERRGSDGGRGGDDYGRGKYSRSPSRSPSRRERSRSPERY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMM5</accession>
    <name>G3AMM5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Solute carrier family 66 member 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_137163</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MPDU1 (TC 2.A.43.3) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374984.1">
        <property type="nucleotide sequence ID" value="XM_007374922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMM5"/>
    <dbReference type="STRING" id="619300.G3AMM5"/>
    <dbReference type="GeneID" id="18870021"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137163"/>
    <dbReference type="eggNOG" id="KOG3211">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1016031_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMM5"/>
    <dbReference type="OMA" id="WAERLFT"/>
    <dbReference type="OrthoDB" id="271506at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016817">
        <property type="entry name" value="MannP-dilichol_defect-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12226">
        <property type="entry name" value="MANNOSE-P-DOLICHOL UTILIZATION DEFECT 1 LEC35 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12226:SF2">
        <property type="entry name" value="MANNOSE-P-DOLICHOL UTILIZATION DEFECT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023381">
        <property type="entry name" value="MannP-dilichol_defect-1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR023381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="287" mass="31579" checksum="46533029C6E75C04" modified="2011-11-16" version="1">MSSPIIVLIKAVYSDDISNKVLIQMAFSQAKAIGYGRILAAVLGSATVAVSSFIRIPQIRKLLIKSEKSRISVAEGLSLDGLGLETVNYLIHAVFNAQNKVPFINYGESLLLGIQNAIIILLAKFYRLRAYGEIDDFSNLDCKQKVQAVAKSKVANTLAIMVAATVFFTKIAPPQLISALQILNIPFSVIAKLPQIRQNYKLKTASHLSETVLTANVIGSLIRVYTSFTDYSLKSKRRKDTSNEKILVAGYTASLVMNSTLFGQAIVYDKLNKDTEPTEDKDKKKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMM8</accession>
    <name>G3AMM8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Agglutinin-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_50348</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374987.1">
        <property type="nucleotide sequence ID" value="XM_007374925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMM8"/>
    <dbReference type="FunCoup" id="G3AMM8">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="GeneID" id="18871430"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50348"/>
    <dbReference type="HOGENOM" id="CLU_031316_1_0_1"/>
    <dbReference type="InParanoid" id="G3AMM8"/>
    <dbReference type="OrthoDB" id="3981162at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2430">
        <property type="entry name" value="Agglutinin-like protein, N-terminal domain, N2 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008966">
        <property type="entry name" value="Adhesion_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024672">
        <property type="entry name" value="Agglutinin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043063">
        <property type="entry name" value="Agglutinin-like_N_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033504">
        <property type="entry name" value="ALS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011252">
        <property type="entry name" value="Fibrogen-bd_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793:SF2">
        <property type="entry name" value="AGGLUTININ-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793">
        <property type="entry name" value="ALPHA-AGGLUTININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11766">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01056">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49401">
        <property type="entry name" value="Bacterial adhesins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442508" description="Agglutinin-like protein N-terminal domain-containing protein" evidence="13">
        <location>
            <begin position="18"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Agglutinin-like protein N-terminal" evidence="14">
        <location>
            <begin position="50"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW33472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="335" mass="38018" checksum="9BA4AAFA4E64B88B" modified="2011-11-16" version="1">MIPVFLSIIFLVSFVNGCELFTSLDEVVFITPSMHDPAIPYKPRWDATLSWAINGKEIKPSKRFTMKMPCVYKFCTDKSYIPLIANHVEYGQCYFKSGEIFDKYSKLECVISNNVIKTTNVKGQIRVPLVFNAGGSALCTDRECAKLFKGGSNVIEFSGGKKFRYTVNFTPGLPYNTDKGVFHIRGVPSKNKVQPYLVAGNCPQGYSAGYFGFEIRDSMTKIDCCSYHAKKSKYNEFNSWMFAKTEHSISKKASCGNNRLEVTYKDICKDYRPYLDALLVPTVGHPVKIYYYNRWMCKGDPTEHRNDGYYEWTPESCDPTNVVTTTLPTATVVPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMV9</accession>
    <name>G3AMV9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Endoplasmic oxidoreductin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">SPAPADRAFT_61451</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">May function both as a monomer and a homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EROs family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375649.1">
        <property type="nucleotide sequence ID" value="XM_007375587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMV9"/>
    <dbReference type="FunCoup" id="G3AMV9">
        <property type="interactions" value="774"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMV9"/>
    <dbReference type="GeneID" id="18873901"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61451"/>
    <dbReference type="eggNOG" id="KOG2608">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023061_1_0_1"/>
    <dbReference type="InParanoid" id="G3AMV9"/>
    <dbReference type="OMA" id="CYKDRLH"/>
    <dbReference type="OrthoDB" id="269384at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016972">
        <property type="term" value="F:thiol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007266">
        <property type="entry name" value="Ero1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037192">
        <property type="entry name" value="ERO1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613:SF0">
        <property type="entry name" value="ERO1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613">
        <property type="entry name" value="ERO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04137">
        <property type="entry name" value="ERO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017205">
        <property type="entry name" value="ERO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110019">
        <property type="entry name" value="ERO1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 17" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442440" description="Endoplasmic oxidoreductin-1" evidence="20">
        <location>
            <begin position="22"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="474"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="505"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="16">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="371"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="18">
        <location>
            <begin position="95"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="18">
        <location>
            <begin position="368"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EGW32373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="23">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="595" mass="68378" checksum="9D49737AC1A2265B" modified="2011-11-16" version="1">MKFNTFLTLVLSNVFISSVLATVHQYPKASFCPFDSPEFCSQEVTSSCNTTFSYIDNLNRQIRPDLKSLIETTYFRYFKVDLEQQCKFWDAQHFCASENCAVEIDEEFNWTQVTNEEFKPFKLGALKLPGKDDKVDNPIETEEVQVCEDLDYCHIDDSHECVYVDLQKNPERFTGYGGNQSFDVWKAIYSENCFPNTNPMSMIGGQQEEQCVEKNLFYRVISGLHASIAVHLSNEYLNSVTGEFYPNLKIFMERVGYFNERLANIYFNFALVSQSLVKLNEILPLKEFILSGYDDITPVQQQQLLANNFTDKAEVYDELLFDDIIPALSSNLLFNTSTLFGPGVDPNLKNEFRARFKNVSAIMDCVGCDRCRMWGKIQTIGYGTALKILFESEDPNNKDRLKFRRIEIVALVNTLDRLSKSIEAINNFKQMYLEHVRNVALGISKPEEMDKKFDTNSGFAFPFVSPILPETSKPVEKSEPELQSAPVQPEAAKSAKKVEQSSSVEPREESHDSIEEIAKIPPSERTFKQELRLAFDEIWQALKFIFNSYKNFPVILGKLGLVKADSWWNEFIGKPVRQYESRVDVDAQQYEAMIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN43</accession>
    <name>G3AN43_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_71868</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell septum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the formin homology family. BNI1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375733.1">
        <property type="nucleotide sequence ID" value="XM_007375671.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AN43">
        <property type="interactions" value="495"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN43"/>
    <dbReference type="GeneID" id="18875398"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_71868"/>
    <dbReference type="eggNOG" id="KOG1922">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001313_1_0_1"/>
    <dbReference type="InParanoid" id="G3AN43"/>
    <dbReference type="OMA" id="NHYWKAR"/>
    <dbReference type="OrthoDB" id="1104827at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000142">
        <property type="term" value="C:cellular bud neck contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001411">
        <property type="term" value="C:hyphal tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000920">
        <property type="term" value="P:septum digestion after cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2220:FF:000006">
        <property type="entry name" value="Cytokinesis protein sepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000898">
        <property type="entry name" value="Formin BNI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.150">
        <property type="entry name" value="Formin, FH3 diaphanous domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051661">
        <property type="entry name" value="Actin_filament_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014767">
        <property type="entry name" value="DAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010472">
        <property type="entry name" value="FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010473">
        <property type="entry name" value="GTPase-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47102">
        <property type="entry name" value="PROTEIN BNI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47102:SF2">
        <property type="entry name" value="PROTEIN BNI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06367">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06371">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01139">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01140">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51231">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="10">
        <location>
            <begin position="152"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="11">
        <location>
            <begin position="1132"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="domain" description="DAD" evidence="9">
        <location>
            <begin position="1568"/>
            <end position="1598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="47"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="759"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="796"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="947"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1022"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1547"/>
            <end position="1572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1602"/>
            <end position="1673"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="625"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="94"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="766"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="796"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1031"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1057"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1088"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1106"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1562"/>
            <end position="1572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1659"/>
            <end position="1669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1701" mass="193124" checksum="742DFE8B8A58A170" modified="2011-11-16" version="1">MNNTGPQTPNHRISTSSIFSIRKTKDKHPNDSNSDLSSNDSASILSASTQPSFTRRQSSLALSKTATNRSSTSANTSIVPTNQVVQSQHNRQASTFSISTINNTSPPQPSKSLKSTLSRKSTTNSMATPIISRPSKTSNDLSLPQSGGFVLERPDSIYEIDRMFKDLMEKRDFKSLPTQAKQEMLNYSHDKKWMLIYQDAYAEHTRQERNSRSTKTGQIENTASPQYYAKLLLSKKITAKELKDLWVSLRTEPIDWVRRFIYDCQGDAALSAYLKMVQDEISSADIHDINDEKFDREFNTLKALKCMMNQKLGAERVRTDANLYVNAISGSLLSPRIITRKIAAEALTFMIAYYSHHNVDSGKYHKILHALDSIPQKPHFEFEQLNDQRTAGAGRLVRKPPSPEKYKRFELWLRLVERTVDGKGKYMNSLVGASEELKSAHAGTSGTTTAASLENHLVEYCLGTMLLINTIVEYGADYRIRIRLRAQFNAAGLDRLIAKFQELGYDSLNQQCMKYIQMSQLDEEELKTSEHIDENLDFNNPVDLVNALWDNVKNSEAQGYFLSAIQHLYLNQSEKRNNVEEMGRSLRLLDGLIQNIATAHTTNDESAVGIAINRLFANMSTDDMYRKALEEVKVYKRLAEEATAERDEMSRQLSMGADGLITNLQNDVREQDIVLTRTRRLVEELTQELNQLKRKHLQEKQEQELEMRELLILLNSKDFDTEIETKREKKGKTTVAIKTTSEELAKTIRKNIHRRQAEYRLDNRNFGGTSVEPSTRLQSLRDMMDTLGNEARELEMSEFDDDILTEESVPEEIPEEAESDEEEDEVEESLEEPETPTGPKRGVRSDDLAKLDKLRKKLASLQTESNDIMKFNNSSMFNRQKFLAMERLRDLEENFKDFNIDFSVDDQDNLHEHFSSPERVDPSIKDKIKEEWEEIARLKLELKRKLADAETKKKSTKPKSNKDTLARIEEKYVRGKIKNESTADIVRASTTTENKRAHRMTTIGAMDPVFLKELSSKVGRVEPIDVSSAASPPPPPPPPPPPLPDMFQGSDSVTSKSPPPPPPPPPPPPPPPPPPPAPEALLSGKTSPAPPPPPPPPPGLFNGAIPPAPPLPLPNSPKNIPDPIASRHNSLFDNIPRPKKKLKQLHWEKLDDGQAKNSFWKDPNVHTLASDLMERGVFDEIELIFAAKEVKKLATKKKDETEKVSFMPRDIAQQFSINLHAFSSLSDEELVTKILRCDRDVLHNQAVLEFFSKDEIVEVSNSLARNLEPYSTDYTVDEVSKPDKDPNELERPERIYLELIYNLQHYWKSRMRALKVITNYEKDYEDLVSKLRLIGDAVQGIKESKHLKQVFEIILTVGNYMNDSSKQAQGFKLSSLQRLSFMKDDKNSMTFLHYVEKIIRTQYPELLEFMEELSKCIEVSKFSIENIKSDCTEYNQSMKNVQSSIDVGNLSDISKFHPLDRILKVILPVLPRAKKKGELLIDQSNYTLKEFDDLMIYFGEDPSDQFVKNSFMSKFANFIKDFKRVQTENLKREEEIRIYEQRKKLLEAPRKASSSANGTDGDSNDDGDDNDVMDSLLEKLKATGPPKGEAISARRRALMRKHLAEDSKSPASPARNGDDSNEDDGSNRSSPIKKRDGEVSPSDVEFGARAKNLLQELRGGSDEHLDEKISSAQKFRQERLRKKLTAVGDLDLESDNNNGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANA0</accession>
    <name>G3ANA0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytidyltransferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_61552</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 12">
            <text>beta-nicotinamide D-ribonucleotide + ATP + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375760.1">
        <property type="nucleotide sequence ID" value="XM_007375698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANA0"/>
    <dbReference type="FunCoup" id="G3ANA0">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANA0"/>
    <dbReference type="GeneID" id="18873948"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61552"/>
    <dbReference type="eggNOG" id="ENOG502RXY8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032651_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANA0"/>
    <dbReference type="OMA" id="RLVMMEL"/>
    <dbReference type="OrthoDB" id="536881at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034355">
        <property type="term" value="P:NAD+ biosynthetic process via the salvage pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02165">
        <property type="entry name" value="NMNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005248">
        <property type="entry name" value="NadD/NMNAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31285">
        <property type="entry name" value="NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31285:SF0">
        <property type="entry name" value="NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="9">
        <location>
            <begin position="41"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="269" mass="30597" checksum="01AC95AFC6B1CB76" modified="2011-11-16" version="1">MKSKFIKPLQEFIASKSDFSVLYHTSPEFITPATQRICVLDSSFNPPHLGHYALVEESLKNTFGSVGQSNDNKIVLLLLSIKNADKAAPKPESLEHRLQMMYLMANDISKKWNISVSIGVTKHAKFVDKSVSCLNFIKPSSTLKLTFLVGFDTLVRILNPKYYLPDKLSTSLEQFMKSTDLFCLTRVDKDITNDEQNNYLEEIRGGRHEDIPSHWGENIFLVKNFGDDIANISSSEIRLQISQGNDDWKSKVVPEIRDFIVNNDLYKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANF6</accession>
    <name>G3ANF6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_138075</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375221.1">
        <property type="nucleotide sequence ID" value="XM_007375159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANF6"/>
    <dbReference type="FunCoup" id="G3ANF6">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANF6"/>
    <dbReference type="GeneID" id="18870094"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138075"/>
    <dbReference type="eggNOG" id="KOG3508">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002973_1_0_1"/>
    <dbReference type="InParanoid" id="G3ANF6"/>
    <dbReference type="OMA" id="WFVGLNY"/>
    <dbReference type="OrthoDB" id="775356at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05137">
        <property type="entry name" value="RasGAP_CLA2_BUD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF60">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN RASKOL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="389"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="4">
        <location>
            <begin position="589"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="710"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="724"/>
            <end position="736"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW31945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1149" mass="131146" checksum="1117DC7891DD09A4" modified="2011-11-16" version="1">MNKHPSPFHKIISRSKGTFTGRNFLVSADSVSWTHVHTLKLTSEGQLYSYDEDDTNQILLLQSLQACYIQIYPNISTSTLNSSGSNPSLGTVPENSVKKGFIRKAAEAATATATTHTSDLAVDESQLPVIFVKTYDNDKLYIKIPSQSNFGCLLASLVVWQNLRPSGLAKKWYCQNKHRRNLPSSELLVCRFKIYGPIPPKYHRNLNIVPGYPAPVYQARIEDDISGVNGSGTMTSDVNEGWFYTMGALNSSGVLNFISELDGTLLYSIDVRQIMSSEIREVHNSIFNNSNVLFIGQLKELRHNNVIKTISNLSADQYSQPFLTRDGKPVPSNQRILIEFPLHIDLEDWFVGLNYFCKREYIGSFNDKTKHLGGDVFNPVAPVKSSNSLVEPPKMSEFTKSSFRVSKKISIDIIEAKFDNMPYRASGKIYAETRMWGYPWSRTAPVNFTANPFWKEHFSTDLPISTQMIHILIKRCNNRSNIPSSSDKLIGTVYVTPDILTNQINSSSTIINMGEAANGNSHLATGDIVRLTIHDSNNIPIGKLVVDVNLREFHILSPAHFKPLERMLANPTPMKDLIQFCNKHVPSSDFENVSLILLDIFQSLGVEDAWFKNLIETELVHVDMTTRKNYHKRNSDQNPKKHERGLSSSTNVFNTLFRGSSIFSKSLEKYNLRIGQEYLEKVFGDFFVKISRENKNCEVDPRYVRLQERARRKGRTVEQQQESSSDDDEDEEDEEEIDDDYIDQLVNQNFNNLMSYVEEIWYKIYITSNDIPKQIKLQLKNFRTKVELACDPSDHTTSLNCISAFIFLRFFCPAILNPKLFHLTKNHQTGTAQRTLTLIAKILLNLANRQEFTPLKEPHLVKMNSFITSHTDEIYDYFDKITDRKNDFNERILNLSHDVKRFDLGLDDSTSSELPTTPYLIDKYLRLTEIIHILDYSKNSPGTTLTSSPANGHVEMFRDIKVDHERNVYQIGSLEFEKSDFLDLAGDNETEGFIKSLCQNNEEIFSFISSNITLQDLQKQSTKLMAKIHTLERYLDCSEYPINFQSVDPLLWDSFTYDILNNRVYLDTTRNCIISVDVPPPPSQYKRLAESNALASLKLRFTTTSSNFRNSMVDSDSVSRISLSFSSHSLGIGRGSNTKNPFKKWLRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANH0</accession>
    <name>G3ANH0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ras modification protein ERF4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_71503</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Interacts with ERF2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERF4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375235.1">
        <property type="nucleotide sequence ID" value="XM_007375173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANH0"/>
    <dbReference type="FunCoup" id="G3ANH0">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANH0"/>
    <dbReference type="GeneID" id="18875382"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_71503"/>
    <dbReference type="eggNOG" id="ENOG502S30T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087349_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANH0"/>
    <dbReference type="OMA" id="CITHFPN"/>
    <dbReference type="OrthoDB" id="5377273at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031211">
        <property type="term" value="C:endoplasmic reticulum palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019383">
        <property type="entry name" value="Golgin_A_7/ERF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051371">
        <property type="entry name" value="Ras_palmitoyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13254">
        <property type="entry name" value="GOLGI AUTOANTIGEN, GOLGIN SUBFAMILY A, 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13254:SF0">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 7_ERF4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10256">
        <property type="entry name" value="Erf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Golgin subfamily A member 7/ERF4" evidence="9">
        <location>
            <begin position="65"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="232" mass="26372" checksum="550ACE4F216475FF" modified="2011-11-16" version="1">MSTTNDNNSNPKLFSSGPSTSEPNDLSFFNYHEFLVPQSKYSLVINHYPNNYASSNSTTQLNTRIIRIPRCYETTEFSDLIPSFSLYYPGKEPGAITQEHLDVQGTYDGNIFGITSDFRLEINSNELQEIVRQVNQYLYNAFYPYSIPTLFENVLDLITGGMFSQLSNLVGVRSHTKRTLLQLEQYITDVNSKFKEREIDVVIISPRKSGYLSVCIAFCFAKVGILLTYSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANI8</accession>
    <name>G3ANI8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flo11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_153562</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375793.1">
        <property type="nucleotide sequence ID" value="XM_007375731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANI8"/>
    <dbReference type="GeneID" id="18870958"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_153562"/>
    <dbReference type="eggNOG" id="KOG1565">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_689958_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANI8"/>
    <dbReference type="OMA" id="PTTTDCE"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018789">
        <property type="entry name" value="Flo11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51824">
        <property type="entry name" value="FLO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flo11" evidence="2">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="400" mass="43033" checksum="5096723FB6B72A9A" modified="2011-11-16" version="1" fragment="single">MQQINEFTCTTPFEISYLWQEAGATYDHGCDFLDGVGDLPLLCWEEICERVEQTTTEEESNTSIEPSTTQTEAETTSDTQDETVSDPQDETTSDTQVTSSDPQDETSSDPQDETTSDTQVTSSDPQDETSSDPQDETTSDTQVTSSDPQDETTSDTQVTSSDPQDETSSDPQDETTSDTQVTSSDPQDETSSDPQDETTSDTQVTSSDPQDEPTIVTQDETTSDTQVETTSDPQDETRSIEKTTEPTSENTEDPVKSPAEEETKSSSSETTILVSQESTMTQSDEPSESPTQTPAQSGTESSTYSASHQATGAPTEESSNPGSASAFTTNVSTNRETETKTNDPSNRNTETSTDSATGNQSIPPTIATSRTVIETITTTSVDPTITRTITNTVTYITADP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANK6</accession>
    <name>G3ANK6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cdc6/ORC1-like ATPase lid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61599</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375811.1">
        <property type="nucleotide sequence ID" value="XM_007375749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANK6"/>
    <dbReference type="FunCoup" id="G3ANK6">
        <property type="interactions" value="1112"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANK6"/>
    <dbReference type="GeneID" id="18873970"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61599"/>
    <dbReference type="eggNOG" id="KOG2227">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012774_2_1_1"/>
    <dbReference type="InParanoid" id="G3ANK6"/>
    <dbReference type="OMA" id="MDSELFF"/>
    <dbReference type="OrthoDB" id="1926878at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054425">
        <property type="entry name" value="Cdc6_ORC1-like_ATPase_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF26">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22606">
        <property type="entry name" value="Cdc6-ORC-like_ATPase_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cdc6/ORC1-like ATPase lid" evidence="2">
        <location>
            <begin position="90"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="301" mass="33930" checksum="1E34B9C11AD24D55" modified="2011-11-16" version="1">MDSELFFNLLNKQDKFKSIIVILDELDSLITRDQQVLFELFQASNIINSHRLKTKLVLIGISNTLDLTDKFLPRLIRNNLSPETLQFLPYTADQIKAIIISRLRSLTGNDSESEIPIFHPSAIQLCSRKSASISGDLRKAFDICYKSIELVERNLPPFQTFTDFNSTNYPKVMIPHVAKVCTLSFDNNSIGNLNLLQKALLCHLFNYQLENLGSSDLSVNSFYDYYRKQLNTNNLLGLLKIGEFIEIISALESSGCIVVNDKRKGGSGMGNKILKLNVGYDDIVKSIEDVGLLKKLLKSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP53</accession>
    <name>G3AP53_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pseudouridine synthase I TruA alpha/beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_61166</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375360.1">
        <property type="nucleotide sequence ID" value="XM_007375298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP53"/>
    <dbReference type="FunCoup" id="G3AP53">
        <property type="interactions" value="725"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP53"/>
    <dbReference type="GeneID" id="18873753"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61166"/>
    <dbReference type="eggNOG" id="KOG2554">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014673_2_0_1"/>
    <dbReference type="InParanoid" id="G3AP53"/>
    <dbReference type="OMA" id="YFGWEYN"/>
    <dbReference type="OrthoDB" id="25767at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02569">
        <property type="entry name" value="PseudoU_synth_ScPus3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.580:FF:000020">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.660:FF:000012">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041707">
        <property type="entry name" value="Pus3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF5">
        <property type="entry name" value="TRNA PSEUDOURIDINE(38_39) SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="6">
        <location>
            <begin position="192"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="411" mass="47054" checksum="6FE1F86A5826C930" modified="2011-11-16" version="1">MTDYSSWSKEQLIAKLTELESCAAGAPAKKQKKQKEFDWSKYHTRFVAIRFAYLGWNYNGLAYQKEETPLPTVEGTILDALHKAKLIKEPTVDCCKFSRCGRTDKGVSAMNQVISLNVRSSLTPEEQADSSNDVKEVKYATILNSLLPPDIRITAISLRPPPKFDARFSCESRHYKYLFLKQQLDIDAMNQAAQLYEGTHDFRNFCKVDGSKQISRYERDIYSAKIMPVDGIPDMYCFDLKGSAFLWHQVRCMVAVLFLVGQGLEPVSIVSDLMDVDKFPTKPQFEMANDAPLVLYDCTFPEMEWLSLSDMQALYQSKWSWTSFQGLVYDHQIKAQMAQIMDQIVLSDKAAIQTHNKQMMNTGDGAGRAFASYIPIVDRERGEHFEVANAKWLERKSRKKEEEEEEQVDTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3APL1</accession>
    <name>G3APL1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_152755</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375458.1">
        <property type="nucleotide sequence ID" value="XM_007375396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APL1"/>
    <dbReference type="GeneID" id="18870935"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_152755"/>
    <dbReference type="HOGENOM" id="CLU_1031204_0_0_1"/>
    <dbReference type="InParanoid" id="G3APL1"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="270" mass="31489" checksum="4EBCF7EC5C13AB01" modified="2011-11-16" version="1">MESFDRTIESIQSILRTLSVSLSKSQLKQILMDINHNWFSYTTNQFSRSFNNGVIINWNYKQGPNIILSLLQVFSDEANYAFNEPFSSKYLETIKQMCDDPYKFNLSKLSHYDVCQLHKINSEGTLIYHFGTIYRYLVFILLGILMFEQMVAIFKFPISEVVFIKKIVLGVIQSVLLFFYYKWNVMNALLQDFLKINTIGILQMNHGIFILAIVCLQLTTYWVFQSELIRLRNTQKEFPVSANIRALQIWGLCAFAVCGVVPLIVLIFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ22</accession>
    <name>G3AQ22_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-histidine N-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_61422</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the methyltransferase superfamily. METTL18 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.85" evidence="3"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375619.1">
        <property type="nucleotide sequence ID" value="XM_007375557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ22"/>
    <dbReference type="FunCoup" id="G3AQ22">
        <property type="interactions" value="1619"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ22"/>
    <dbReference type="GeneID" id="18873886"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61422"/>
    <dbReference type="eggNOG" id="KOG2920">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038704_1_1_1"/>
    <dbReference type="InParanoid" id="G3AQ22"/>
    <dbReference type="OMA" id="ADVKFQM"/>
    <dbReference type="OrthoDB" id="1723750at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018064">
        <property type="term" value="F:protein-L-histidine N-tele-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045903">
        <property type="term" value="P:positive regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF39">
        <property type="entry name" value="HISTIDINE PROTEIN METHYLTRANSFERASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="344" mass="38510" checksum="BB1448C437D4B7FF" modified="2011-11-16" version="1">MSFSFGFTNDDLSGDELDQSSTPSQPPQARALDTLVTPKENLPQRHTLAQLCSTLLNVRITFDNYTTPGGNLIYRRELFDVKHQIMSEDEGSTQLGEMLLDEQSDLQRNVYEGGFKSWECAYDAVDKLASGQVEMSSVLEYGCGTALPSCFILLRKFTSGDKNSLRITLSDFNYDVLRLVTLPNLIINWASTLPVEKLHALTTSEENPVFNNDEVLLTQQLIQEFTSALNEFNIELDFISGAWGNQFNELVTSQTSVDFMISSETIYSPDILPVVAESIVEILSRTNGTAKCMVAAKNIYFGVGGSVIEFVNYFDKVSESKFIMEVEEINDSQLKRSLVMIEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ60</accession>
    <name>G3AQ60_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_51425</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376185.1">
        <property type="nucleotide sequence ID" value="XM_007376123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ60"/>
    <dbReference type="STRING" id="619300.G3AQ60"/>
    <dbReference type="GeneID" id="18871553"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51425"/>
    <dbReference type="eggNOG" id="ENOG502SD49">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049045_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQ60"/>
    <dbReference type="OMA" id="DHIVRDD"/>
    <dbReference type="OrthoDB" id="4023202at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.440">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036517">
        <property type="entry name" value="FF_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81698">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="404" mass="47588" checksum="F3FBF17CC47FF119" modified="2011-11-16" version="1">MTPQFVYKLPLQEWHLIITNTGHHFYFNEKTSKSYWQLSDIQALGIDVETFLNGVNFDELALLFAKARGLKIDEVVENQEEIEWRSEEVEIVIERGDVVSGEKEKEVRELNHEPESEQTQIIQGYSSSEEDYEDEEEEKEENVSRPEQDLDIDQLAATAIEQYQHSDSETDSEASNDLDLSFDDNSSSEDFISLLEQFKSKISPFEPWELIEERLISEFVKYPEFYTIDSKSKREEIFRQWSKQQEDEEEQDQEEEEVVNIYPTPTIEYLAFLQNFKSEIKNGFYQPFYSKHYKSINSFNLTPQQKETIFRNYKLMLSESSIAEREYKKANSKSGNFKVMKLTEFLKQFNLSGGKVNYNSDESFFTNWIDLLNVNQVDLEIAENQINFLVGDEKRFVSYKSALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ93</accession>
    <name>G3AQ93_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">ATP-dependent RNA helicase DBP5</fullName>
            <ecNumber evidence="4">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">ATP-dependent RNA helicase dbp5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="ORF">SPAPADRAFT_140222</name>
    </gene>
    <organism evidence="30">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">ATP-dependent RNA helicase associated with the nuclear pore complex and essential for mRNA export from the nucleus. May participate in a terminal step of mRNA export through the removal of proteins that accompany mRNA through the nucleopore complex. May also be involved in early transcription.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22 31">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Associates with the nuclear pore complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the DEAD box helicase family. DDX19/DBP5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="4"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376219.1">
        <property type="nucleotide sequence ID" value="XM_007376157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ93"/>
    <dbReference type="FunCoup" id="G3AQ93">
        <property type="interactions" value="794"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ93"/>
    <dbReference type="GeneID" id="18870280"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140222"/>
    <dbReference type="eggNOG" id="KOG0332">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_0_1"/>
    <dbReference type="InParanoid" id="G3AQ93"/>
    <dbReference type="OMA" id="IAAETRW"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
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        <property type="term" value="F:inositol hexakisphosphate binding"/>
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        <property type="term" value="P:protein transport"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="DEADc_DDX19_DDX25"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SF2_C_DEAD"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
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        <location>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="494" mass="55173" checksum="3CC2A9C6EB166DB6" modified="2011-11-16" version="1">MSNEKEKRVDADASELLASLSIDGAKKETSTKEETKKATDDDKAETTDKKVETEKEKPKKEEEVKIEDKEESPKESNLIKSSYEVKVKLADLQADPNSPLYSVKSFEELGLSPELLKGLYAMKFNKPSKIQEKALPLLLSNPPENMIGQSQSGTGKTGAFSLTMLSRVDESIPATQCICLGPTRELARQTLEVITTMGKFTKITSQLVVPDSLERGKATNAHILVGTPGIVIDLMRRKLINTSKVKVFVLDEADNMLEAQGLGDQCLRVKKQLPKSCQLVLFSATFPDEVRAYAEKFVPNANSLELKHEELNVEGIKQLYMDCSSEEHKFQVLCELYGLLTIGSSIIFVERKATADKLYMKMKQEGHTVSVLHGGLENESRDKLIDDFREGRSKVLITTNVLARGIDIASVSMVVNYDLPTDKNGQADPSTYLHRIGRTGRFGRVGVSISFVHDKRSYDILMKIRNYFGGIEMTRVPTDDWDEVEKIVKKVIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQB6</accession>
    <name>G3AQB6_SPAPN</name>
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        <recommendedName>
            <fullName evidence="4">Transcription factor PAP1 domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="5" type="ORF">SPAPADRAFT_62032</name>
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        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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    </organism>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_007376179.1"/>
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        <property type="interactions" value="3485"/>
    </dbReference>
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    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62032"/>
    <dbReference type="eggNOG" id="ENOG502RPD7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="InParanoid" id="G3AQB6"/>
    <dbReference type="OMA" id="ENIFFKH"/>
    <dbReference type="OrthoDB" id="5380163at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="YAP1 redox domain. Chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013910">
        <property type="entry name" value="TF_PAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023167">
        <property type="entry name" value="Yap1_redox_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF6">
        <property type="entry name" value="AP-1-LIKE TRANSCRIPTION FACTOR YAP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08601">
        <property type="entry name" value="PAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="YAP1 redox domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor PAP1" evidence="4">
        <location>
            <begin position="18"/>
            <end position="261"/>
        </location>
    </feature>
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        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
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        <location>
            <begin position="170"/>
            <end position="207"/>
        </location>
    </feature>
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        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="170"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="192"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW31463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="268" mass="29708" checksum="EE344D49D34756BB" modified="2011-11-16" version="1">MNKDVNNNLDFTAGFDEQLDSFCAKLGEACGTRQQPVPKYKRNNSSTSSVPVSHQYNSPFSTLVTPNSNSYDYTNDPFFTTNGDFNFNIPSPKDVKTDASIAKAAEKNDPLSFLNDNNFDVSLAFGDPSPGDELDPIALLTTEESQYDPLTDQVNVNFNFNDFVKHSLPSESSVSTGNYTTEQPKVSTIPEVQEEDDDDDDAVVPAPPKTIKCSEIWDRITAHPKYTEIDIDGLCHELKSKAKCSENGVVINATDVNKLLEENIFFKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQH4</accession>
    <name>G3AQH4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mRNA stability protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_62103</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Plays an essential role in initiation of the G0 program by preventing the degradation of specific nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the endosulfine family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376299.1">
        <property type="nucleotide sequence ID" value="XM_007376237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQH4"/>
    <dbReference type="FunCoup" id="G3AQH4">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQH4"/>
    <dbReference type="GeneID" id="18874186"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62103"/>
    <dbReference type="eggNOG" id="KOG4076">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101493_2_1_1"/>
    <dbReference type="InParanoid" id="G3AQH4"/>
    <dbReference type="OMA" id="IYRMYGK"/>
    <dbReference type="OrthoDB" id="5949865at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006760">
        <property type="entry name" value="Endosulphine"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358">
        <property type="entry name" value="ENDOSULFINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358:SF6">
        <property type="entry name" value="ENDOSULFINE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04667">
        <property type="entry name" value="Endosulfine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="103"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="120"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="131" mass="14533" checksum="C999C759AACC5006" modified="2011-11-16" version="1">MSLSEDDPLGPPKNTQNLDLSKLTPQELKIYKMYGKLPTKQQILSGRFQDKKYFDSGDYAMQKQLGGGFRSGMPGGMPMRHPNAERMREMSVRNSISSTNAQIFANGKSGLLNDATPDKAEEESDASTEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQY3</accession>
    <name>G3AQY3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_51244</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375990.1">
        <property type="nucleotide sequence ID" value="XM_007375928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQY3"/>
    <dbReference type="FunCoup" id="G3AQY3">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQY3"/>
    <dbReference type="GeneID" id="18871538"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51244"/>
    <dbReference type="eggNOG" id="KOG2214">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009031_4_1_1"/>
    <dbReference type="InParanoid" id="G3AQY3"/>
    <dbReference type="OrthoDB" id="10049244at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07230">
        <property type="entry name" value="Pat_TGL4-5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021771">
        <property type="entry name" value="Triacylglycerol_lipase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF10">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11815">
        <property type="entry name" value="DUF3336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="6">
        <location>
            <begin position="193"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="597"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="860"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="4">
        <location>
            <begin position="224"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="613"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="632"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="860"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="911" mass="101894" checksum="292E3520832B6CD4" modified="2011-11-16" version="1">MNESEEIIQSIFKQYIELVPENNRTSEDSPSWINSVPGLGRILSGANENSSQFDKLLEERNRATSFSQWRDISFKLDELSGNNAWKSDPKSDMYDYELIFNNMNEMKNARLSHDYKLLLYLIRTKWTRNIGNMGNSHLYRHSYVGTKTLIEEYINECKTCLDYLLNSKDVDLDDRYLLGMLIQTRKNIGRTALVLSGGSTFGVFHIGVLATLFEANILPRIISGSSAGSIMASILCAHTQEETIDLLSSITDYEFNIFDIADSGEISDLPVLKRVLHYLGHLIKYGTVFDIEGLKQTVISFLGDLTFREAYNRTGKILNITVSAASVHEQTRLLNYLTAPNCLIWSAVCASCSLPGIFPSVNVYEKNTKTNEIQEWNYDSSMKYYDGSVENDLPITRLSEMFNVDHIIAVQVNPHVVPVMKSSLWEVGSGVRKELHQARTKFKNIFNSICEFAGCEIVHYLQVLSELDICKDLSNKIVSILSQDYSGNINIIHHYKPADLIKILTNPDAKFLLEYFCRGARATWPKVPIISNNCGVEFALDKSISMLRGRLITSANNRITYNIPNNEVKKSRSFHRGLPKANVILPTSDSVEIKITPERSKHANRPAALHRFSSASGNLNASRKLTMRTRRNNSISGGSSNSPTTPTTPNTPKKKITRGISSYTLLQLTSDSSNLLLGDSETGEATINIRRSGSTGSFRFPIDAVSATVPGNDKSLKFKSARIPFLKNNPYFDFGGGDELLGLSEKREEQKEKINLPSVSRSNSKHNSYIGLNRLKGNDLSRSSDPSNNTLNSASIQDIAKLMEGPRSALKNCTKSHSSLDSMDSTDDELYKLESTPSIRVVDCKLDDDNETTLAHDEEVYFGDENEETEGESNSIKECQGNGKNEESRYNTEDGCLISEPALRKSTSFQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARE7</accession>
    <name>G3ARE7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">glycogenin glucosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.186</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_140797</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Self-glucosylating initiator of glycogen synthesis. It catalyzes the formation of a short alpha (1,4)-glucosyl chain covalently attached via a glucose 1-O-tyrosyl linkage to internal tyrosine residues and these chains act as primers for the elongation reaction catalyzed by glycogen synthase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-tyrosyl-[glycogenin] + UDP-alpha-D-glucose = alpha-D-glucosyl-L-tyrosyl-[glycogenin] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23360"/>
            <dbReference type="Rhea" id="RHEA-COMP:14604"/>
            <dbReference type="Rhea" id="RHEA-COMP:14605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:140573"/>
            <dbReference type="EC" id="2.4.1.186"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[1,4-alpha-D-glucosyl](n)-L-tyrosyl-[glycogenin] + UDP-alpha-D-glucose = [1,4-alpha-D-glucosyl](n+1)-L-tyrosyl-[glycogenin] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56560"/>
            <dbReference type="Rhea" id="RHEA-COMP:14606"/>
            <dbReference type="Rhea" id="RHEA-COMP:14607"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:140574"/>
            <dbReference type="EC" id="2.4.1.186"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glycosyltransferase 8 family. Glycogenin subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.186" evidence="10"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376532.1">
        <property type="nucleotide sequence ID" value="XM_007376470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARE7"/>
    <dbReference type="FunCoup" id="G3ARE7">
        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARE7"/>
    <dbReference type="GeneID" id="18870319"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140797"/>
    <dbReference type="eggNOG" id="KOG1950">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017171_4_2_1"/>
    <dbReference type="InParanoid" id="G3ARE7"/>
    <dbReference type="OMA" id="KILIWKQ"/>
    <dbReference type="OrthoDB" id="2014201at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008466">
        <property type="term" value="F:glycogenin glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02537">
        <property type="entry name" value="GT8_Glycogenin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000092">
        <property type="entry name" value="Glycogenin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="313"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="327"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="351"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW31754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="404" mass="46286" checksum="AEB2E3EB951446EF" modified="2011-11-16" version="1">MSNFAYATLLTGESYLPGVLTLGQKLKELGTKHKLIVLLDSSSISEENKEVIQSIYDEIIPIDEEVISAPLEKVQEKLDRSELSITFSKILLWNLTQFDELVYLDADVLPLQNLDELFESFELKSGEIAASPDSGWPDIFNSGVLKIKPSTETFEKLIEFSSQPENTFDGADQGLLNEFFGGNNWVRLPYLFNVTPNYRQDYQYLPAFHRFFNQIRILHYIGAVKPWHYGDILSSDLANFHQYWWDDFNRFFGEDAHLKYKLLNLPRGEASNLKFGKQKNAWDVDFVTGGSSEPTRESHSPVFPWEHREEKREATRVFHPTTPPSGEVEEGSSEAVEEDLKKNIEALGDVKVSKDTEKKSEPLSKNYGFEKEGDKNFNPEKSLAEVAKLPFKFFSKARGKKEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARF0</accession>
    <name>G3ARF0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thiamine-binding protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_56534</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0045 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376535.1">
        <property type="nucleotide sequence ID" value="XM_007376473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARF0"/>
    <dbReference type="FunCoup" id="G3ARF0">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARF0"/>
    <dbReference type="GeneID" id="18872044"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56534"/>
    <dbReference type="eggNOG" id="ENOG502S45I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137479_0_2_1"/>
    <dbReference type="InParanoid" id="G3ARF0"/>
    <dbReference type="OMA" id="RVIGQCH"/>
    <dbReference type="OrthoDB" id="5587367at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029756">
        <property type="entry name" value="MTH1187/YkoF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002767">
        <property type="entry name" value="Thiamine_BP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051614">
        <property type="entry name" value="UPF0045_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00106">
        <property type="entry name" value="MTH1187 family thiamine-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33777">
        <property type="entry name" value="UPF0045 PROTEIN ECM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33777:SF1">
        <property type="entry name" value="UPF0045 PROTEIN ECM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01910">
        <property type="entry name" value="Thiamine_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89957">
        <property type="entry name" value="MTH1187/YkoF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine-binding protein" evidence="2">
        <location>
            <begin position="9"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="107" mass="11719" checksum="DAF8495FF7DAFA3D" modified="2011-11-16" version="1">MPVTIHCLADVCLIPIGTGSASVSDEVTAITKLAQDSPLETTLHSAGTTIEGPWNEVMDLIGQFHQHLHDKHGVVRIQSDIRVGTRTDKKQCPQDKIDVVQQKLKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARH3</accession>
    <name>G3ARH3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_141577</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376072.1">
        <property type="nucleotide sequence ID" value="XM_007376010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARH3"/>
    <dbReference type="GeneID" id="18870384"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141577"/>
    <dbReference type="eggNOG" id="ENOG502RAHE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1390705_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARH3"/>
    <dbReference type="OMA" id="ICYVIAP"/>
    <dbReference type="OrthoDB" id="4013261at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="186" mass="22184" checksum="6F7B74761A2BB749" modified="2011-11-16" version="1">MHFHSLYVVIDNIKYICYVIAPTLSFVYLVTKFINPDSLESLSQNFQVQLFSQPFCESRPNECAMTGDKILCKYFKYYDMVYYRYYLIAQWDRCILYTLQTSWMKWLALYLGSKDISDEKIWEKWLETLEIVLAIMNSITLYTIKRLVVSKMWLYFIFSTILAVSKPWAVVANKLQIVLRTTNNVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARX5</accession>
    <name>G3ARX5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="20">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">SPAPADRAFT_140785</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 20">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38567"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:38568"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Membrane</location>
            <topology evidence="20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="20"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376602.1">
        <property type="nucleotide sequence ID" value="XM_007376540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARX5"/>
    <dbReference type="FunCoup" id="G3ARX5">
        <property type="interactions" value="303"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARX5"/>
    <dbReference type="GeneID" id="18870317"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140785"/>
    <dbReference type="eggNOG" id="KOG0206">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000846_3_0_1"/>
    <dbReference type="InParanoid" id="G3ARX5"/>
    <dbReference type="OMA" id="MHSFWSW"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0140345">
        <property type="term" value="F:phosphatidylcholine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="F:phosphatidylethanolamine flippase activity"/>
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        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
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        <property type="term" value="F:phosphatidylserine flippase activity"/>
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        <property type="term" value="F:phosphatidylserine floppase activity"/>
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    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
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        <property type="term" value="P:endocytosis"/>
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        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
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    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
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        <property type="entry name" value="Phospholipid-transporting ATPase"/>
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        <property type="entry name" value="Phospholipid-transporting ATPase"/>
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        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
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    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
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    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
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        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
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    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
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        <property type="entry name" value="HAD-like_sf"/>
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    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
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    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
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    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
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    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF150">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 18 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 19 20" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 18 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 20" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="265"/>
            <end position="287"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="315"/>
            <end position="336"/>
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    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="841"/>
            <end position="860"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="872"/>
            <end position="893"/>
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    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="913"/>
            <end position="944"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="956"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="984"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1022"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="21">
        <location>
            <begin position="6"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="22">
        <location>
            <begin position="805"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="17">
        <location>
            <position position="379"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
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        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
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        </ligand>
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        <location>
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        </ligand>
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        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00004166"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00008109"/>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022840"/>
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            <dbReference type="ARBA" id="ARBA00022842"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00034036"/>
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            <dbReference type="ARBA" id="ARBA00051303"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
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    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="EGW31824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1132" mass="128712" checksum="F8021C6CB82E67CE" modified="2011-11-16" version="1">MDHGLNAPYGYYGNYISTTKYNFATFLPKFLFEQFSKYANLFFLFTSIIQQVPHVSPTNRFTTIGTLIVVLLVSAIKEIMEDIKRANADKQLNNTKVQVLDAESGSFVWKKWIKVQVGDVVKVNNEEPFPADLLLLSSSEPEGLCYIETANLDGETNLKIKQAKTETAYLVNPRDLLSDLHDAEIVSEQPNSSLYTYEGNLRNFRNGSVRDIPFTPEQLLLRGATLRNTQWIHGVVIFTGHETKLMRNATATPIKRTDVERIINLQIIALFCVLITLSLISTIGNVIKTRVDNSSLGYLYMEGTSTAKLFFQDILTFWILYSNLVPISLFVTVELIKYYQAFMIGSDLDMYYEETDTPTGVRTSSLVEELGQINYIFSDKTGTLTRNVMEFKAVSIGGKCYIEEIPEDGYPQIVEGGIEIGFHTFNELHQDLKNTNTQQSAIINEFLTLLSTCHTVIPEITESDKIKYQAASPDEGALVQGAADLGYKFIIRKPRYVTIENTLTTMQSEYELLNICEFNSTRKRMSAIFRCPDGVIRLFCKGADTVILERLSEDEPQPFVNSTIRHLEDFAAEGLRTLCIASRIISEEEYESWSATYYEASTSLDDRSDKLDAAAELIETNLFLLGATAIEDKLQDGVPETIHTLQNAGIKIWVLTGDRQETAINIGMSCKLLSEDMNLLIINEETKDGTRMNLQEKLTAIQDHQFDNEDGSFESTLALIIDGHSLGFALESDLEDLFIELGSRCKAVVCCRVSPLQKALVVKMVKRKKKKSLLLAIGDGANDVSMIQAAHVGVGISGMEGMQAARSADISIGQFKYLKKLLLVHGTWSYQRISNAILYSFYKNITLYMTQFWFVFTNAFSGQSIMESWSLTFYNVFFTVLPPFVLGVFDQFVNARLLDKYPQLYQLGQQRKFFNVAVFWSWITNGFYHSAVIFLCSFLIYRYMNVLSTGLTADNWSWGTAVYTTCTLTALGKAALIVSLWTKFTLIAIPGSFIFWLLWFPIYSTVAPMTKVSQELRGVLRATYPSITFWSMIFGVAVLCLLRDFAWKFYKRRYSPETYHYVQEIQKYDIQDQRPRMEEFQRAIRKVRQVQRIKKQRGFAFSQVEGQDQEKIVRLYDTTKKRGVFGELSEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS04</accession>
    <name>G3AS04_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Genetic interactor of prohibitins 3, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Found in mitochondrial proteome protein 38</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_51824</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in the mitochondrial lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376631.1">
        <property type="nucleotide sequence ID" value="XM_007376569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS04"/>
    <dbReference type="FunCoup" id="G3AS04">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AS04"/>
    <dbReference type="GeneID" id="18871598"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51824"/>
    <dbReference type="eggNOG" id="ENOG502S067">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025792_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS04"/>
    <dbReference type="OMA" id="IIPPFYG"/>
    <dbReference type="OrthoDB" id="1696305at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
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        <property type="entry name" value="Mito_lipid_metab_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46434">
        <property type="entry name" value="GENETIC INTERACTOR OF PROHIBITINS 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46434:SF1">
        <property type="entry name" value="GENETIC INTERACTOR OF PROHIBITINS 3, MITOCHONDRIAL"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
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        <location>
            <begin position="149"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031834"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="590" mass="67456" checksum="1AC7C30411B44759" modified="2011-11-16" version="1">MLTNLIRSSLKRNFVRFTSTSNVALPFLYDLEPKCRSCGIRLQNKDETKPGYYHLRSKQEKEQQKLESSPKTAYEKYMAKLSPEDKELLLGNIFTGAPANHTPKSGPSVKELAKRAYDIKLDETAIECARCRNAKVHSDYKLSQEEFPIHDLNAIMPKIPSDSPIVYVFSGNDFPMGINADIFKYRNPDEVYFIMTKADNLFKKSDAARNFCFTFLTDYFRIKYQVPAKNIYITSAKEGWNSIDLLNFIPESAYIVGNVNSGKSTLIKSLMLHQELENKRDLESDIPFHIKAREKKKFIDRFNQKVGPGISYLPGFTREFLPVTLGGVRSVFDVPGFASDPYIHEFYHRFSDGKTIARLTGGKQTYKFGTYKSKYDSVKGPQVLGLGGIGYLQLPQDSMYQIRNVTNIDYHVFSNIERAVKVSINPSDSLKKKFIIEHDEASLVNFERYVIPPFYGQIELVLENIGYIHIKPVGAKKTNELMVLYLPRGITGMIRQPIVEYIQKTFKDSWEQNLIKGKRGGKGNLVLKRYKGDTPYASILIPATGKITDATGENQSDFDQLNAMTAAKRPYNDKTVINEENKYEYWIEPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS08</accession>
    <name>G3AS08_SPAPN</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62469</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007376573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS08"/>
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    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62469"/>
    <dbReference type="eggNOG" id="ENOG502RQES">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="InParanoid" id="G3AS08"/>
    <dbReference type="OMA" id="HNYLANV"/>
    <dbReference type="OrthoDB" id="4094421at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="111" mass="12642" checksum="A693392C036068C6" modified="2011-11-16" version="1">MTTEENKPEQQQQQQPEDPQITKEKLEELRLKIKEGINSAGEHPLITSYVDSTFSYLQNIHDKMTAAPDKKAAAQEIADEITSKVENWMKSRQQASTADSKPIEEKPSEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASJ5</accession>
    <name>G3ASJ5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Na+/H+ exchanger CtNHA1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_51893</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fungal Na(+)/H(+) exchanger family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376714.1">
        <property type="nucleotide sequence ID" value="XM_007376652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASJ5"/>
    <dbReference type="FunCoup" id="G3ASJ5">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASJ5"/>
    <dbReference type="GeneID" id="18871605"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51893"/>
    <dbReference type="eggNOG" id="KOG4505">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008635_0_1_1"/>
    <dbReference type="InParanoid" id="G3ASJ5"/>
    <dbReference type="OMA" id="WPITCFF"/>
    <dbReference type="OrthoDB" id="5327978at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1530.20:FF:000015">
        <property type="entry name" value="Na(+)/H(+) antiporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013928">
        <property type="entry name" value="Cation/H_antiporter_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004712">
        <property type="entry name" value="Na+/H+_antiporter_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382:SF4">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08619">
        <property type="entry name" value="Nha1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="102"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="414"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="13">
        <location>
            <begin position="26"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Alkali metal cation/H+ antiporter Nha1 C-terminal" evidence="14">
        <location>
            <begin position="462"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="489"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="666"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="777"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="515"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="557"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="567"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="590"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="689"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="811"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="822"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="840"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="870"/>
            <end position="904"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW30681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="929" mass="104277" checksum="D226087B63AD2658" modified="2011-11-16" version="1">MVWSQLELEAPHIAYACVGIFSTLFSLVSLFVKERLYIGEATVATIAGLIIGPHCLDWFNPVTWGNTDYITLEICRIVLCIQIVAVAVELPRKYMKKHWVSVTIFLLPVMTFGWLTVGLFIWCLIPHFSFSDGLLVAACVTATDPVLAAAVVGKGKFAQRVPGHLRNLLSAESGCNDGMAFPFIFLALYIITHRGEPGLIVKDWFLLTIFWECLFGCLLGAVLGYVLRKLVAFAERRNLIDRESFLAIFVFLAFNSAGIGSMLGVDDLLVSFAAGTAFGWNGDFAKKTEESHVSTVIDLLLNLSFFVYFGAIIPWPQFNNPAIGLDVWRLVILAFVIIFLRRLPAVIAFKPFTPDVKTWREALFCGHFGPIGVGAIFAAILARKDLEAHYTTEETPLAHLPNEEFPHYQLLACIWPMVCFIVITSIVVHGSSVAVLTLGRRLNRMAITMSFTTTGEGNGPTWMQRLQKLDKTSTTFSLHRVDTIAVTEKEKPTDEQGVATGVETSGIKVRPAGGAKRRAQLKKKKKKKKKKPAHIIKKTLSKVTTGGMSDEEEEEEYEHKTDRQRPQTEFLQLGRSNNLAPSETSEDTVEEKPSSPKEDTSVETEETMQGGVPDKTSGMKKYGQRPPTEEEKRSIPTVAYQDGDQIIIEDQYGEIMDSLKLQQRAKPAPVEPVESFTNINDDDDASVHSEASLQRRLSQYASADSEYSFNDEKQEQDDTDLSPIRKRISRSISKKTKVLYRKDDPHRKKVYAHQVDDLIVIENEEGEIIRRYKVNRHHSKFRNPPQEQEGGTRPRSTTLVGKMKSILGFNESEPTSPTTPTIQVSNYDLEQGNSNMIIPHEGDEGDTKLEHKLSNMLSKKKSPPPRVLSDEDEEDSNESQDSEEDVSEVDSEVDSEEDSDEHETEVEKRRRLQALGKLPVHRHQDDEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASP6</accession>
    <name>G3ASP6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">Multifunctional fusion protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="10">Delta-1-pyrroline-5-carboxylate dehydrogenase</fullName>
                <shortName evidence="10">P5C dehydrogenase</shortName>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">L-glutamate gamma-semialdehyde dehydrogenase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="9">L-glutamate gamma-semialdehyde dehydrogenase</fullName>
                <ecNumber evidence="9">1.2.1.88</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_63037</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 9">
            <text>L-glutamate 5-semialdehyde + NAD(+) + H2O = L-glutamate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:30235"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="EC" id="1.2.1.88"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.88" evidence="9"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377143.1">
        <property type="nucleotide sequence ID" value="XM_007377081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASP6"/>
    <dbReference type="FunCoup" id="G3ASP6">
        <property type="interactions" value="389"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASP6"/>
    <dbReference type="GeneID" id="18874564"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63037"/>
    <dbReference type="eggNOG" id="KOG2455">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_4_1_1"/>
    <dbReference type="InParanoid" id="G3ASP6"/>
    <dbReference type="OMA" id="FAGIHFT"/>
    <dbReference type="OrthoDB" id="5322683at2759"/>
    <dbReference type="UniPathway" id="UPA00261">
        <property type="reaction ID" value="UER00374"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003842">
        <property type="term" value="F:L-glutamate gamma-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000006">
        <property type="entry name" value="1-pyrroline-5-carboxylate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000005">
        <property type="entry name" value="1-pyrroline-5-carboxylate dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005931">
        <property type="entry name" value="P5CDH/ALDH4A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050485">
        <property type="entry name" value="Proline_metab_enzyme"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01236">
        <property type="entry name" value="D1pyr5carbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42862">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42862:SF1">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0520">NAD</keyword>
    <keyword evidence="3 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 9" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="11">
        <location>
            <begin position="98"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="590" mass="64929" checksum="23E071A55FFF9295" modified="2011-11-16" version="1">MLRSTACRSIRVAAASRCTTKILLPTITTVVRRTASTHAAQLSHIKSPPFLANEPVRPFSFKDTKDWDLLRASLTKFTDEGALSVPLVINGKKIYRDETKPQVNPSHHSQNLANVSQATAEDIKAAIEAAKSAKAKWAATSWTDRAAIFLKAADLISTKYRYDMLAATMLGQGKNVYQAEIDCVAELIDFFKFNVKYAEEMYGTQPIQTAPGVWNRAEYRPLEGFVYAVTPFNFTAIAGNLVGAPALMGNTVVWKPSATAALSNYLLLTILEEAGLPAGVINFIPGDPIQVTDLVLEDPEFSALHFTGSTDVFKNLYCKISNNVAADKYRDFPRIVGETGGKNFHLIHPSASINHSVLSTLRGAFEYQGQKCSATSRAYVPESIWPEFKDQIAAAVDTITIGNTSDPSQLNAFMGPVIHQQSFDKLKAVLDNAKTDPELEIITGGSYDSTNGYYVQPTVIRTTNPQHEYLTKEFFGPILTVYVYPDNSYEEIMNSIDGATKYGLTGSVFARDREAIRLAEEKLRYAAGNFYINDKSTGAVVGQQWFGGARASGTNDKAGSGNILSRFVSVRSVKENFYELTDFKYPSNYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASW1</accession>
    <name>G3ASW1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein eL40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_62600</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376776.1">
        <property type="nucleotide sequence ID" value="XM_007376714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASW1"/>
    <dbReference type="SMR" id="G3ASW1"/>
    <dbReference type="STRING" id="619300.G3ASW1"/>
    <dbReference type="GeneID" id="18874397"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62600"/>
    <dbReference type="eggNOG" id="KOG0003">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175093_1_0_1"/>
    <dbReference type="InParanoid" id="G3ASW1"/>
    <dbReference type="OMA" id="KGATNCR"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1060.50:FF:000001">
        <property type="entry name" value="ubiquitin-60S ribosomal protein L40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001975">
        <property type="entry name" value="Ribosomal_eL40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038587">
        <property type="entry name" value="Ribosomal_eL40_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01020">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01377">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL40" evidence="5">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="52" mass="6061" checksum="D509F0BFA3F7F641" modified="2011-11-16" version="1">MIEPSLKALASKYNCEKSICRKCYARLPPRATNCRKRKCGHTNQLRPKKKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT01</accession>
    <name>G3AT01_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_62643</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376816.1">
        <property type="nucleotide sequence ID" value="XM_007376754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT01"/>
    <dbReference type="FunCoup" id="G3AT01">
        <property type="interactions" value="1220"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AT01"/>
    <dbReference type="GeneID" id="18874417"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62643"/>
    <dbReference type="eggNOG" id="KOG1076">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004304_0_2_1"/>
    <dbReference type="InParanoid" id="G3AT01"/>
    <dbReference type="OMA" id="MIISNSE"/>
    <dbReference type="OrthoDB" id="29647at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027516">
        <property type="entry name" value="EIF3C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008905">
        <property type="entry name" value="EIF3C_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13937">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITATION FACTOR 3, SUBUNIT 8 EIF3S8 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13937:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05470">
        <property type="entry name" value="eIF-3c_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="5">
        <location>
            <begin position="298"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="495"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="508"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="549"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="561" mass="64847" checksum="D53CA781A3CD7808" modified="2011-11-16" version="1">MLLSIRFDAGALQTYMSFDLWHKNQQDLLKLLQLLESNIATYQVSESGELTDDIDIEPTENEAGVKVIFGSITSLIDRLDDEFIKSLQNIDPHSIEYVERLKDEITIYNLIVRGQAYIELITEDFNSSEQLGRIVLTRLEHIYYKPNQLIKLNEEEVWKNITSDSKIVSKESTPTEVINGLAKYLINHTNSMYGDYAKLFSIYHYAVNNQYTEAYELFMNSQFYNTINNSESTLQTQYNRALVQLGLSAFRSGQIEESHRILNEIVNSQRSKELLGQGFISKYSNQITNQEKAKLLPFHQHINLELLECVYSTCSLLIEIPQLALSSKDNSKRKSSVKSFKSKLEFHDRQFFTGPPESIKDHIIHASIALTKGDWAKSYELLSSIKIWKLFPDFDELLKMLKDQLQVEGLRTYIFTYKTIYNKLSLDKLSQIFQLSNERIKEILEKIIESDDVSGEFNDVNGKVFINFVSSEPRRSKLQELAIVMNEKVGLLAEKNDKTGSNGYGKKQPQQQQQQQQQQQQQQQAQQSQQPQQTPQPTEEQPNRFRYANVNSNNDEFQASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATC1</accession>
    <name>G3ATC1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_142475</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376917.1">
        <property type="nucleotide sequence ID" value="XM_007376855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATC1"/>
    <dbReference type="GeneID" id="18870450"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142475"/>
    <dbReference type="eggNOG" id="KOG1286">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_12_2_1"/>
    <dbReference type="InParanoid" id="G3ATC1"/>
    <dbReference type="OMA" id="AMFSTAN"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000001">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF3">
        <property type="entry name" value="AMINO-ACID PERMEASE PB1C11.02-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="361"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="431"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="465"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="42"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="536" mass="60487" checksum="608093400746587C" modified="2011-11-16" version="1">MSDSIEKSSDAIKHDLELVDAVSSLNEVTKTESLNRSLSTRHVNMITIAGVIGTGLYLGTGKMLATGGPLSLLLNYAIIGLVVFFMMLSLGEMSVQFPISGSFTVYAKRFGNDSLGFAILLNYWFNDCCSVAGDLVALQLVFQYWTDFHWWVISLIFWVFLLALNVIHVRLYGETEYWLALLKVITILIFFIVSIICNAGKNDSGSYIGFKFWSYGDAPFVDGIRGFSSLFVSAAYAMGGLESVSLTAGETVNPVKVIPRTIKATFWRIMIFYIFTAFFIGMNIPYDYPNLMTKTVATSPFTIVFQQVGAKGAGSFMNAVIMTSILSAGNHALFAGSRLAFNLGSQGYLPKFFTRLNRFKIPYVAVIITWLVGGLCFGSAFVGAGTLWSWLQAIVGLSNLISWWLIAVISIRFRRGLAKQNRTHELLFRNWTYPIGPWFVVLFGGFIIIIQGWSTISPFDVSGFFQSYLELLVFPVTYIFWWLIIRRGKDRFVKKEDMDFDTDRYFQSPEELEEKEYAQSLKGWAKFKYNFVDNFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATJ3</accession>
    <name>G3ATJ3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_68179</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376989.1">
        <property type="nucleotide sequence ID" value="XM_007376927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATJ3"/>
    <dbReference type="GeneID" id="18875201"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68179"/>
    <dbReference type="eggNOG" id="ENOG502T3J5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_332929_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATJ3"/>
    <dbReference type="OMA" id="NPILNWI"/>
    <dbReference type="OrthoDB" id="4019088at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="859" mass="101968" checksum="B8BEE8DC63DF30AA" modified="2011-11-16" version="1">MGTPDPSEELASSNAMVKPEASTPRNRTPDLTTSIENGSSLSTSSTNKTGLQTPETSPRFQVPGDVLKNLTPSRSMKSPEKQQQAIKKRQAHLDSIRHRLKRKSLELSNRLQILQFKRQEETRRRINRINKDLHEKKQRRSKYLDLIRERAGKFYLRNVSILFKPTITPIKKYKQDVVEAPILVDTGFITDIQRKCRLILFRKYIATLEESKFLDEFESYSFNKIVEILNTNSTIKTSMNFILKYLNVPRLFGAQYKSFFYCFILVGDFNDCMEFEKHPGFNANNSLYESELYFYNSIWLLVYKFTKLLINEFKLVVRNRKLHDNFISLWYDYNFIFKIFKWNHYRNICDILSDSLAIVDQQITVSESNEGKLQLMQQKSKLAQELSLLNSYNIEELAKFNETVEVVQFKKSFSNIVAEFYAKPTHHVLIQNKTRVIQFSTFKFYVPTCEILKTSEWRKFWFDKFRRQEDNREFPETIRSGYIKNLIERNVDEFIHVDDIVSRKVTDDVEFRLPQIYNDLREYYIEFMKAKNDIDLDYIDLDTNDNYYLKLMEELIRHYDYDNSILLDLTAKIYQNGIISKRVIQELETYVCNLWINKSKFTEFAKVQQFENIYTLINTVNFSKLRFNIFTSSPNLLFQNFYQMILKYNQPMLDLSEDTQRRILHSAFKNSVSRVLISADKENSRQFFTRLFYKIIVYEDNLQPDELNGLFCENFTKFHTRILQLIDATVFRILYSNYIYPSDRSRARLTKVEVQTLVTRLQNTKDVASVLQAANIADFGFFKYYTKSHETFKTLLQGKVFNMLQSGKSKEAYIIIEGNFPDFNGEVLELANDVWKMTFFVYKLYNPILNWIYIDLGMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATJ8</accession>
    <name>G3ATJ8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Translation machinery-associated protein 16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_142119</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TMA16 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376994.1">
        <property type="nucleotide sequence ID" value="XM_007376932.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATJ8"/>
    <dbReference type="FunCoup" id="G3ATJ8">
        <property type="interactions" value="356"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATJ8"/>
    <dbReference type="GeneID" id="18870424"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142119"/>
    <dbReference type="eggNOG" id="ENOG502RY79">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106785_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATJ8"/>
    <dbReference type="OMA" id="WAYIATI"/>
    <dbReference type="OrthoDB" id="270284at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.170">
        <property type="entry name" value="Translation machinery-associated protein 16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021346">
        <property type="entry name" value="Tma16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038356">
        <property type="entry name" value="Tma16_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349:SF2">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11176">
        <property type="entry name" value="Tma16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="20943" checksum="749820D66720552E" modified="2011-11-16" version="1">MPLAHNLNKVTKNLSKSTGQMHIKGRKFKQLNRATLRDKKINERKLKTLQQKENELAIVFFLQKLINDDDDFKVQETFSLEEIKEFIELFIGRFDDELEELRAARRPGRPATSRQQILEEKVKHDRQVYATGVKVPDLADKETVQRLRLWNGTTGGATIMKFVHVSKDMTEFATAEVEMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATQ6</accession>
    <name>G3ATQ6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_52382</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377253.1">
        <property type="nucleotide sequence ID" value="XM_007377191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATQ6"/>
    <dbReference type="GeneID" id="18871671"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52382"/>
    <dbReference type="eggNOG" id="ENOG502RQ1S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_759031_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATQ6"/>
    <dbReference type="OMA" id="NESHTTN"/>
    <dbReference type="OrthoDB" id="4082794at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="365" mass="41580" checksum="50BFE5D343AB2E4F" modified="2011-11-16" version="1">MNMDKENAVSNALKQLSINKQKSPTLDDHLDLIHTTQEEIQAQLHNIEVQSTQTSVDLAQLFDRSKNNNQNLNRLLENVVAYSKEVMTEGNATKADMNRIFDKLEQLETVGKEETGEVANMVKGLLEALNKQYKEVGGNTVREVKEFHSGLKQSFQCEVGDIGASLSGIKDISEELKSGIKKNKDTLDSLTEKIPNVEHISDPIVREIKSLNSNSEATLQKILDRIAQLEKPQNYQEQFNLILSKLSKFEETRAEQDNKRQIELTNKQQELESKISHLENKYSTLCNSYTQKFHEYQQLNEKYTTLVKQIETLDVPTDMTRLKKVRQFHSSTPTTNFSAKQKRIASTPVPRSADSDFINNSDEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU24</accession>
    <name>G3AU24_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Multidrug resistance protein 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_57191</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377371.1">
        <property type="nucleotide sequence ID" value="XM_007377309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU24"/>
    <dbReference type="FunCoup" id="G3AU24">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU24"/>
    <dbReference type="GeneID" id="18872102"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57191"/>
    <dbReference type="eggNOG" id="KOG0255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008455_11_5_1"/>
    <dbReference type="InParanoid" id="G3AU24"/>
    <dbReference type="OMA" id="HELQAPM"/>
    <dbReference type="OrthoDB" id="9986881at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000082">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF7">
        <property type="entry name" value="DRUG_PROTON ANTIPORTER YHK8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="361"/>
            <end position="382"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="388"/>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="419"/>
            <end position="442"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="454"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="40"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="491" mass="55069" checksum="E20F24E272222BCE" modified="2011-11-16" version="1">MAEELEIESQKEDDPFLVKFDGTDDKEDASRLSRLHKWSIVGIISFGSLCVTCISSSWSLASPQIMKHFHISHEVSTLGISLYIWGLGTGGIFLSPISEFHGRRLVYIFGILITILFELVTTFSDNIGAMLFGRFMSGFAGSSFMSVASGSFADLFKANKAHDGKKDANKELALAMVLYSVSPFVGPGLGPLISGFINSSMNFIWTFIVLLLWSALLLILVIFFVPETYEPINLKRKAKRLRKQTGDDRYYAPIDKTEITLYHSVVISSKRPILLIFRDNMTMVLCFYTGFTLAVVYMFFVAFPFIFQTVYNFSLSEQGMSFLGLVVGMISTCLISPYFIDKLYLKLLHRNNGVSKPEFRFIPLMIGVFIVPIGLFIIAWTAYPSVHWIAPIIGSAVYGCGTILVFNGIFAYTVEAYRLYAASAMATNSFIRSIMSGVFPLFGLQMYKAMGIHWATTLLALFAVVLIPIPFLFFKYGEYLRSKSPYAWSTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU88</accession>
    <name>G3AU88_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 17">Aurora kinase</fullName>
            <ecNumber evidence="2 17">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">SPAPADRAFT_143307</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
            <location evidence="1">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2 17"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377436.1">
        <property type="nucleotide sequence ID" value="XM_007377374.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU88"/>
    <dbReference type="FunCoup" id="G3AU88">
        <property type="interactions" value="1084"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU88"/>
    <dbReference type="GeneID" id="18870533"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143307"/>
    <dbReference type="eggNOG" id="KOG0580">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="InParanoid" id="G3AU88"/>
    <dbReference type="OMA" id="ESRFPEW"/>
    <dbReference type="OrthoDB" id="377346at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032133">
        <property type="term" value="C:chromosome passenger complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005828">
        <property type="term" value="C:kinetochore microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051233">
        <property type="term" value="C:spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045143">
        <property type="term" value="P:homologous chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045144">
        <property type="term" value="P:meiotic sister chromatid segregation"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044779">
        <property type="term" value="P:meiotic spindle checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044774">
        <property type="term" value="P:mitotic DNA integrity checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901925">
        <property type="term" value="P:negative regulation of protein import into nucleus during spindle assembly checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090266">
        <property type="term" value="P:regulation of mitotic cell cycle spindle assembly checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901673">
        <property type="term" value="P:regulation of mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000235">
        <property type="entry name" value="Serine/threonine-protein kinase ark1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0137">Centromere</keyword>
    <keyword evidence="8" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="7 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="6 15 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="30"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="157"/>
            <end position="158"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="16">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EGW30465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="300" mass="35124" checksum="504C5963CCF3DAD6" modified="2011-11-16" version="1">MSNSTTSSTPLSSPFVESPGSEKRFTLDDFEFGKILGKGKLGRVYCVKHKESGLVVALKAMNKQELIDLRLEKNFRREIEIQSKLHHPNITRLYTWFHDHVNVYLVLEFSLYGELYHHLKQAKRFDNALASYYIYQVTQALIYLHRKNIIHRDLKPENIMLSLDNVIKLSDFGWSVQQHQDKPHYQSNPTRRITLCGTLDYLPPEMVESKPHDKSVDIWALGILTYEFLVGKPPFEEASKDATHQRISKGDIRFPNYVDHDAVDLIRKLLHKVPEKRLSLKSVLNHRWIMKHKPNWPTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUC7</accession>
    <name>G3AUC7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63323</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377474.1">
        <property type="nucleotide sequence ID" value="XM_007377412.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUC7"/>
    <dbReference type="GeneID" id="18874710"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63323"/>
    <dbReference type="HOGENOM" id="CLU_1994031_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUC7"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="125" mass="14159" checksum="1E36A47B33A0A86D" modified="2011-11-16" version="1">MDQVQKQLCDAKLKLNALHKQHTELVVETNKIKQQVTALKKNHRKFLRSLSKRTCNSRKKKKGKRKHSNSNLTNALIKVEPVDAATCCAAEVTTKPKDEMNLKMDISVKLENDGCNVTYDVGKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUH5</accession>
    <name>G3AUH5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">D-arabinitol 2-dehydrogenase [ribulose-forming]</fullName>
            <ecNumber evidence="5">1.1.1.250</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ARDH</name>
        <name evidence="7" type="ORF">SPAPADRAFT_63109</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; D-arabinitol metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.250" evidence="5"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377232.1">
        <property type="nucleotide sequence ID" value="XM_007377170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUH5"/>
    <dbReference type="STRING" id="619300.G3AUH5"/>
    <dbReference type="GeneID" id="18874598"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63109"/>
    <dbReference type="eggNOG" id="KOG0725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_1_1"/>
    <dbReference type="InParanoid" id="G3AUH5"/>
    <dbReference type="OMA" id="IENFTKW"/>
    <dbReference type="OrthoDB" id="5325318at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047038">
        <property type="term" value="F:D-arabinitol 2-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000240">
        <property type="entry name" value="SDR family oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF115">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE FABG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00066831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00070881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="277" mass="29667" checksum="0325A5A5AF171C9D" modified="2011-11-16" version="1">MDSYSNVVPNFRLDGRLAILTGGSGGLASVVSRALVAQGADVALIDINLERTQQAAKDVLAWAGATLKGAHESPIGKVSAWSCNIADAESVEATFTAINEHHGKIADLLINSAGYAENFPAEEYPAKNAENIMKVNGLGSFYVSQSFARPLIANKKRGSIILIGSMSGTIVNDPQPQAMYNMSKAGVIHLARSLACEWAKYNIRVNTLSPGYILTPLTKNVISGHTEMKDAWEGKIPMKRMAEPKEFVGSILYLASDSASSYTTGHNLVVDGGYECW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUM2</accession>
    <name>G3AUM2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_63415</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377549.1">
        <property type="nucleotide sequence ID" value="XM_007377487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUM2"/>
    <dbReference type="STRING" id="619300.G3AUM2"/>
    <dbReference type="GeneID" id="18874738"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63415"/>
    <dbReference type="eggNOG" id="KOG0322">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045414_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUM2"/>
    <dbReference type="OMA" id="LVCCNTQ"/>
    <dbReference type="OrthoDB" id="7668193at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="283" mass="31626" checksum="F3A86CF7E62E4B7E" modified="2011-11-16" version="1">MPVNSLNFSNVVMLKNYLITPATTDANNFDVYQLDHKDSYEITRVITSFNGYKLVNNNGITDDYGGRNDLGIIMKMLVIDNILYVGYESGDLLALNIDFEPPIIITKANDAPSTGKLSAFLKQSKTVINRDPKITLVDHINIHAPNPIISLSHSNNHIIAGSTTNKLSLDHDILKLNNSGIQSILPTHKVLLIGYWNGIIEGYSDNQCVFTNKRQLPRVNILDSNKGNAKQENSLLVKLTFMVSRDKIESKAVSKYSIKQKFTDQELVFVGFEDGTIYVYIVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUM6</accession>
    <name>G3AUM6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">5',5'''-P-1,P-4-tetraphosphate phosphorylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_63419</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377553.1">
        <property type="nucleotide sequence ID" value="XM_007377491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUM6"/>
    <dbReference type="FunCoup" id="G3AUM6">
        <property type="interactions" value="211"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUM6"/>
    <dbReference type="GeneID" id="18874741"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63419"/>
    <dbReference type="eggNOG" id="ENOG502QRAQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049915_1_0_1"/>
    <dbReference type="InParanoid" id="G3AUM6"/>
    <dbReference type="OMA" id="GLWFFNS"/>
    <dbReference type="OrthoDB" id="10267950at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003877">
        <property type="term" value="F:ATP:ADP adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009163">
        <property type="entry name" value="Ap4A_phos1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043171">
        <property type="entry name" value="Ap4A_phos1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045759">
        <property type="entry name" value="Ap4A_phos1/2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019200">
        <property type="entry name" value="ATP_adenylylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38420">
        <property type="entry name" value="AP-4-A PHOSPHORYLASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38420:SF1">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_5G14690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19327">
        <property type="entry name" value="Ap4A_phos_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09830">
        <property type="entry name" value="ATP_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000846">
        <property type="entry name" value="ATP_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ap4A phosphorylase 1/2 N-terminal" evidence="3">
        <location>
            <begin position="64"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="ATP adenylyltransferase C-terminal" evidence="2">
        <location>
            <begin position="200"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000846-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="319" mass="36229" checksum="FF98E3777037338F" modified="2011-11-16" version="1">MSNYNIKDDFYDILSSKYEEAINNGHILFNGDSAVDEIEHIKVGNSTVDIQTTLLKSLMHRPEQGSEKSNPFAKPEPELTIVEKFGAKDEFRLVFNKFPVVPKHFMLITREFKSQNSPLTGTELLSTYNILLQLRKLKPTEDWFAFYNCGPESGASQPHKHIQFMTLPPSQNFKAYSDILSSTSHAFIPNVREEPLQNSDLPFAHFVARLPGQSEDITEDDLSLYFVSLLQRSLTVSREHKQDHISYNFIFTPQYMLMVPRSSGKYKDKLGINSCGVQGLLLCKNEELLELVKTDGALSILEKVGFPNTSGKASNEYDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUR6</accession>
    <name>G3AUR6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_142978</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the free Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007377532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUR6"/>
    <dbReference type="FunCoup" id="G3AUR6">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUR6"/>
    <dbReference type="GeneID" id="18870506"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142978"/>
    <dbReference type="eggNOG" id="ENOG502RXXR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077738_1_1_1"/>
    <dbReference type="InParanoid" id="G3AUR6"/>
    <dbReference type="OMA" id="FQGPIAC"/>
    <dbReference type="OrthoDB" id="442947at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033745">
        <property type="term" value="F:L-methionine-(R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Free methionine-R-sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000614">
        <property type="entry name" value="FRMsr_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051330">
        <property type="entry name" value="Phosphatase_reg/MetRdx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021:SF15">
        <property type="entry name" value="FREE METHIONINE-R-SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021">
        <property type="entry name" value="GAF/PUTATIVE CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01320">
        <property type="entry name" value="UPF0067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAF" evidence="2">
        <location>
            <begin position="68"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="177" mass="19420" checksum="95B76D1887233BF4" modified="2011-11-16" version="1">MGEDKHHADYANISSSLSKEETLQHILDSYSALASDTTNWVANLSNCSSLLWHAYHSLNTPVNWAGFYVRSKPEELILAPFQGKVACQIIKFGSGVCGTAASSQKTQLVPDVEKFPGHIACDGETKSEIVVPIVKDGQTLGVLDLDCLDLNGFDKVDQEYLEKLAELISETCDWTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUZ8</accession>
    <name>G3AUZ8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_157957</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377620.1">
        <property type="nucleotide sequence ID" value="XM_007377558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUZ8"/>
    <dbReference type="FunCoup" id="G3AUZ8">
        <property type="interactions" value="410"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUZ8"/>
    <dbReference type="GeneID" id="18871108"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157957"/>
    <dbReference type="eggNOG" id="KOG0198">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002516_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUZ8"/>
    <dbReference type="OMA" id="RFVRYAM"/>
    <dbReference type="OrthoDB" id="266718at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000165">
        <property type="term" value="P:MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000182">
        <property type="entry name" value="MAP kinase kinase kinase mkh1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF48">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE BCK1_SLK1_SSP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="974"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="55"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="146"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="196"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="326"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="378"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="666"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="839"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="154"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="218"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="404"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="666"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="714"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="739"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="847"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="1003"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW29854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1260" mass="142576" checksum="43FAFD1F82B72B12" modified="2011-11-16" version="1">MNNSKRRSVSYDTNRIKLPFSHNNTSRSSSIDSEGVVSNTTGLRTASDGVIYGTSSIQQQQQQQQQQQQQQQQQHQQHPYFHKPTPRGKDEFFNFSPIEPTESTNSFKQVKTQFSFSENDDSLHEFLISPIKSDIFPYRKHSDDTTISPVLESPENTLIESASPPKSATTSRNSGSVTGSLGSIIGSYNYSKSSTELLPKHKQSENEQYDHTRHKSESSTISQGSTTSVPTPKDKRFARYVNQSVNNTSKWQISNVLKWLDIHQFNSSWKETFRKNEISGNRFLELENFDRNSMIWKQFSKYLDLDDNLNSIDRFIELLRKENMNEQTKEHRKSSPIFPTPKQKLPMSATSSSTSLKYIPPQRPYSYIDPKVSSQLPSPSHHSFFRKHNRSTSTDGNSPIVPNFSMSDYKTNGKTKPDSKKGIFSSFRKYGGDKAADLVKHKPQKFPVIDSLPALDANPDFLRPHNSSISSISPISPIERVDEKYLPKVTHTNASILITQDNRHFIPMVITSTEPEHIRKRISQELKLEEYTIHLTEFNATEGAPLPDEVIPKAITNGMNKFLIKQQFKMERRDTGASSDSFELDKTYPATPQYLLNESKAATDYVNCKDLPSIREDPKRPVLQATPPLVIDTNIHQEKSSSPLSAGSLNSFRVIRSQGNEINFDLRRKSPYESKPKSIAMPVKELPEKPLTKKPSVKVSDSIKAKRVAPPPPGDRKIQIKKQDSILRGRSSSTTSYVSNGTNSSNTDTSLQKSKDDSSDDDDFLVKSIKPKFDPKPVASDDDDDDDFFVKPFKKPITKSTPEKPSYTRNAASRMSIRPPVEEVYSNLEKYFPHTNLDKPIIDDSPASPTEATLTRKPTISRTFSNANISPVNPTPTPQPQPQAAKPDNQSLGFRRMKTIRVVANEARRKVLQRKQISPPNTFANITRENSIKLSRSNTKMWGQRVIEVTSREIEQGFVSKLRTRNGNFQEFAWIKGELIGRGSFGDVYLGLNVTTGEMLAVKQVVYNRLNLNGIEALHKEVETMKDLNHVNIVQYLGYEQKNNVYSLFLEYVTGGSIASCLKSYGKFEEVMVKFITKQVLLGLEYLHENNIIHRDLKADNLLLEIDGTCKISDFGISKRSSDDIYSNNANMSMQGTIFWMAPEVIDSLVEGYSAKIDIWSLGCVVLEMFAGKRPWSNEAAISVIYKTGKEKLAPPISEDIKKCVSSQAVDFINKCFTIDPTERPTASQLLTHEFVNSDYDFSFETTKLAEIIKFNSRRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV08</accession>
    <name>G3AV08_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ACB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_57544</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ACBP family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377848.1">
        <property type="nucleotide sequence ID" value="XM_007377786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV08"/>
    <dbReference type="FunCoup" id="G3AV08">
        <property type="interactions" value="420"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV08"/>
    <dbReference type="GeneID" id="18872127"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57544"/>
    <dbReference type="eggNOG" id="KOG0817">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118853_4_1_1"/>
    <dbReference type="InParanoid" id="G3AV08"/>
    <dbReference type="OMA" id="RYKFEAW"/>
    <dbReference type="OrthoDB" id="346910at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00435">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000010">
        <property type="entry name" value="Acyl-CoA-binding domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022408">
        <property type="entry name" value="Acyl-CoA-binding_prot_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000582">
        <property type="entry name" value="Acyl-CoA-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035984">
        <property type="entry name" value="Acyl-CoA-binding_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310:SF62">
        <property type="entry name" value="ACYL-COA BINDING PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310">
        <property type="entry name" value="ACYL-COA-BINDING PROTEIN, ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00887">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00689">
        <property type="entry name" value="ACOABINDINGP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47027">
        <property type="entry name" value="Acyl-CoA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00880">
        <property type="entry name" value="ACB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51228">
        <property type="entry name" value="ACB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACB" evidence="3">
        <location>
            <begin position="2"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="86" mass="9692" checksum="E575A1A584C408BD" modified="2011-11-16" version="1">MVSAEFTAKADAVQNLTTKPSDDELLKLYGLYKQATVGDVNTDRPSAFDFKGKYKWDAWKALEGKSQEDAEAEYIEFASSLIEKYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV27</accession>
    <name>G3AV27_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Replication factor C subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Activator 1 41 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_63720</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377866.1">
        <property type="nucleotide sequence ID" value="XM_007377804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV27"/>
    <dbReference type="FunCoup" id="G3AV27">
        <property type="interactions" value="630"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV27"/>
    <dbReference type="GeneID" id="18874882"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63720"/>
    <dbReference type="eggNOG" id="KOG0989">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042324_1_0_1"/>
    <dbReference type="InParanoid" id="G3AV27"/>
    <dbReference type="OMA" id="GCQSGSF"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902983">
        <property type="term" value="P:DNA strand elongation involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000011">
        <property type="entry name" value="Replication factor C subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000032">
        <property type="entry name" value="Replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000237">
        <property type="entry name" value="replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF20">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="47"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00075373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="346" mass="39276" checksum="39F809057C25DF45" modified="2011-11-16" version="1">MTTTDQYEEERLHHTPWVEKYRPKNLDDVASQEHAVAILKRTLATANLPHMLFYGPPGTGKTSTILALSKQLFGPNLSKSRVLELNASDERGINIVRSKIKNFARLTISNPTPEDLANYPCPPYKIIILDEADSMTNDAQSALRRTMETYSGMTRFCLICNYITRIIDPLASRCSKFRFKLLNNDDGLNRLKYIAEQEDLTLDQEEPVLQQVLDISGGDLRKAITYLQSASKLSNSYDEHQEISGKLIRETAGVLYEEIIDELVNVIKGKNHTQISNYVNEIILQGWSIQQLISQLHDKLILDDSIDSLTKNQIAINLFETDKRLNNGSDEHIQLLNLSLQISKVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV47</accession>
    <name>G3AV47_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">DNA damage-binding protein CMR1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_144487</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-binding protein that binds to both single- and double-stranded DNA. Binds preferentially to UV-damaged DNA. May be involved in DNA-metabolic processes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the WD repeat DDB2/WDR76 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377887.1">
        <property type="nucleotide sequence ID" value="XM_007377825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV47"/>
    <dbReference type="FunCoup" id="G3AV47">
        <property type="interactions" value="745"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV47"/>
    <dbReference type="GeneID" id="18870631"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144487"/>
    <dbReference type="eggNOG" id="KOG4328">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017019_1_1_1"/>
    <dbReference type="InParanoid" id="G3AV47"/>
    <dbReference type="OMA" id="DPNTLYW"/>
    <dbReference type="OrthoDB" id="9890280at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000001">
        <property type="term" value="P:regulation of DNA damage checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050853">
        <property type="entry name" value="WD_repeat_DNA-damage-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14773">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14773:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="312"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="27"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="77"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="498" mass="56859" checksum="40E8F99AD3851785" modified="2011-11-16" version="1">MALSEFERKRQENIQRNKQLLEQLELDTISASLPTRTPTPPTKKRKTATSHKPVKKEVVEPTRRSRRIAGIQTELENPEEYARIQEEQEEKERQKKEIEKLKRTKLFVEDNGQSERVLHLIQSLGDKFSAGDFYEFIRSNESDKSLEEKRKKFDKLHIYKKHDPLDIKMSHGRITAMHFHPATKDRLILGGDTFGNVGIWCVDDTNPIPAITILHPHGRNISKILTPAHSPSKIYSASYDGSVRELDLHKLSTNEVAYLNDPYEKADYPLGVSDINHCIDDPHILYMTTLEGHFYKHDTRTPFKSTSKKGLLRLHDKKIGGFAINPNASYQIATASLDRTLRIWDLRNIGKSTWSEFDQQNSPHAYGSYGSRLSVSCVDWNSDNHLVCNGYDDNILKKDEEEAAIPENLTPFTKIRHNCQTGRWVSILKSRWQPTPADGIQKFVIANMNRGLDIYDQQGQMLARLNESVGAVPAVSTFHPSQNWVVGGSASGKVYLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV91</accession>
    <name>G3AV91_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">nicotinamidase</fullName>
            <ecNumber evidence="6">3.5.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Nicotinamide deamidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_63514</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; nicotinate biosynthesis; nicotinate from nicotinamide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.19" evidence="6"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377660.1">
        <property type="nucleotide sequence ID" value="XM_007377598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV91"/>
    <dbReference type="FunCoup" id="G3AV91">
        <property type="interactions" value="423"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV91"/>
    <dbReference type="GeneID" id="18874789"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63514"/>
    <dbReference type="eggNOG" id="KOG4003">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068979_13_0_1"/>
    <dbReference type="InParanoid" id="G3AV91"/>
    <dbReference type="OMA" id="DFVDSWP"/>
    <dbReference type="OrthoDB" id="3341310at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008936">
        <property type="term" value="F:nicotinamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904524">
        <property type="term" value="P:negative regulation of DNA amplification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019358">
        <property type="term" value="P:nicotinate nucleotide salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052347">
        <property type="entry name" value="Isochorismatase_Nicotinamidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11080:SF2">
        <property type="entry name" value="LD05707P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11080">
        <property type="entry name" value="PYRAZINAMIDASE/NICOTINAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="8">
        <location>
            <begin position="5"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW29894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="229" mass="25654" checksum="50600D67AD87C729" modified="2011-11-16" version="1">MSKVALAVIDLQEDFLPPDGSLAIADGRSIIPKINKLLSKHNWAAIINTQDWHPHNHISFASTHGVAPYSQLQFTHPEGKIDATTNQAQVMTQYVWPDHCVQDTAGAALEQSFADAFNQIQGEKTNVKKGYLVDREYYSCFQDCWGLHHTEMQPYLKEKGITDVVFVGLAYDFCVMNSAVDCAKDGFNTVVLKDYCKSVYPDKIAETDKVYTDAGVRIVEDKDLDALFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVB4</accession>
    <name>G3AVB4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Extradiol ring-cleavage dioxygenase class III enzyme subunit B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_63745</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DODA-type extradiol aromatic ring-opening dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377898.1">
        <property type="nucleotide sequence ID" value="XM_007377836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVB4"/>
    <dbReference type="GeneID" id="18874893"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63745"/>
    <dbReference type="eggNOG" id="ENOG502RYU3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046582_1_0_1"/>
    <dbReference type="InParanoid" id="G3AVB4"/>
    <dbReference type="OMA" id="SVIDGFW"/>
    <dbReference type="OrthoDB" id="7396853at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07363">
        <property type="entry name" value="45_DOPA_Dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.830.10">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014436">
        <property type="entry name" value="Extradiol_dOase_DODA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004183">
        <property type="entry name" value="Xdiol_dOase_suB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30096">
        <property type="entry name" value="4,5-DOPA DIOXYGENASE EXTRADIOL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30096:SF0">
        <property type="entry name" value="4,5-DOPA DIOXYGENASE EXTRADIOL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02900">
        <property type="entry name" value="LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53213">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442392" description="Extradiol ring-cleavage dioxygenase class III enzyme subunit B domain-containing protein" evidence="6">
        <location>
            <begin position="17"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Extradiol ring-cleavage dioxygenase class III enzyme subunit B" evidence="7">
        <location>
            <begin position="65"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="375" mass="42586" checksum="F8D6A4CCCD65C1FE" modified="2011-11-16" version="1">MNLIYTFLSILLPALTYYYYSNKPATTTSTPAISVAPQFTTKVTPQVTEMSSIAKYIKNPTPFPAYFFSHGGPTFMYEDDDFGNKGAWRTVKKIGTKIKNQWKPDYIIVVSAHWQSSGSNLIEISVPSTEDGENKLIYDFGGFPNHMYKEEFHTKGSWFVAEQIRQHLENNKFHSRISKGRGIDHGTWVPFKVAFSDYNTQTKPQPEVKGLDLEETAVIQVSLTSNDKDFETHFKLGQALSKFKNELLWDESKKKYLTGLIVCSGMSVHNLYDLGRAFQQYGGLMPYTKPFNKELTNLVEKSSKDELLPNFLELKKKDILRQAHPTLEHFLPIVVAGGIANQSDEPIKEVYNDAFASLGWGIYQVGNYKKPEATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVG7</accession>
    <name>G3AVG7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_68774</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377682.1">
        <property type="nucleotide sequence ID" value="XM_007377620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVG7"/>
    <dbReference type="FunCoup" id="G3AVG7">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVG7"/>
    <dbReference type="GeneID" id="18875239"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68774"/>
    <dbReference type="eggNOG" id="KOG3359">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008438_2_0_1"/>
    <dbReference type="InParanoid" id="G3AVG7"/>
    <dbReference type="OMA" id="WAPYILE"/>
    <dbReference type="OrthoDB" id="292747at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23283">
        <property type="entry name" value="beta-trefoil_MIR_PMT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF50">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="244"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="305"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="616"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="658"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="687"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="713"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="17">
        <location>
            <begin position="353"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="17">
        <location>
            <begin position="414"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="17">
        <location>
            <begin position="485"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="788"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="989"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1069"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="793"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="804"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="819"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="864"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="890"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="937"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="999"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1025"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1069"/>
            <end position="1085"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW29916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1099" mass="123890" checksum="2B77357475AFC6A0" modified="2011-11-16" version="1">MAKSTTKTAKPKAAVTGAAAAAAAAAAKVSKHQSKQDTVCSVDPIADPVFAKGEVREYLVTEPSPAVLKKRQLLGAKEYWMLSSLLVISLYVRLYDLANPNSVVFDEVHFGGFSRKYILGQFFMDVHPPLAKMLFAAVGAIGGYKGDFEFKNIGDVYPSSTPYVFMRQFPALLGVGTIIFCYLTLRQSGVRPIIAYITSFLLLIENSNVTISRYILLDSPLLFFIAAAIYSWKKFEIQIPFSFGWYRGLVATGIALGLAFSSKWVGLFTIAWVGLLCIYQMWFIVGDLSISPKKAWGHFFARGTILLGVPIALYLFFFAVHFSVLTNTGDGASFMSSAFKAGLKGNKIPRNVTANVGLGSIVTIRHVETSGGYLHSHDAFYPTGSKQQQVTLYPHLDSNNKWFIEPYNGTIYNETFVPLVDGMKIRLRHINTHRRLHSHDEKPPVSERDWQKEVSCYGYKGFEGDPNDDFIVQIVDYRSAKGEAQEKVRAIETVFRLKHAMTGNYLFSSEVKLPEWGFGQQEVSTASSGKRKLTHWYIEENENQWLDSQDADIINYPELTFWQKVVESHARMWKINQGLTEHHHWQSNPYEWPLLLRGINYWARDHKQIYFLGNAVTWWAASASIVTFGIYVVISVFRWQLGQPVSKDKHVFNFNVQTFSYVLGWALHYLPFFIMGRQLFLHHYIPALYFGILALGHFLEIFTGYLTSRSKTLQNIALVLVGLFALFSLAFYFNYSSLIYGTPWTKDACVKSQIIGSWDYDCNNFYNKLSEYDASQPDTVASVTVSEAPAVQAENTPSTTPAANEEKPEHDPLKDTTTTKKKKSAKKDKKKSSKKPADARGPNVIAKPGEPEKKISGPVIDFVPKGEDLERLKRGEKVDIKPVVKKYNDDGEEIEEESPEIVGEPEQLAPPAAVDPETPPEVKSPAEEVESSTQNEESSREVKPKKAKREENKRPPVIDFVPRGEALERLKRGEKIDKYEPVIREFDELKDGAQAGDIDLERGIRKPKEEVKTTTASSSKSNAKKPKESARAVEAKKDKPKRPPVIDFVPRGEALERLKRGEKIESYKPVVREFDEERDGPKEGDPIIPKKNRRDFGAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVI9</accession>
    <name>G3AVI9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Eukaryotic translation initiation factor 3 subunit B</fullName>
            <shortName evidence="7">eIF3b</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Eukaryotic translation initiation factor 3 90 kDa subunit homolog</fullName>
            <shortName evidence="7">eIF3 p90</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Translation initiation factor eIF3, p90 subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PRT1</name>
        <name evidence="10" type="ORF">SPAPADRAFT_63565</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="7">RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7 8">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the eIF-3 subunit B family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377704.1">
        <property type="nucleotide sequence ID" value="XM_007377642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVI9"/>
    <dbReference type="FunCoup" id="G3AVI9">
        <property type="interactions" value="1373"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVI9"/>
    <dbReference type="GeneID" id="18874807"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63565"/>
    <dbReference type="eggNOG" id="KOG2314">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011152_4_0_1"/>
    <dbReference type="InParanoid" id="G3AVI9"/>
    <dbReference type="OMA" id="LWGGPQF"/>
    <dbReference type="OrthoDB" id="10250414at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071540">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3e"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043614">
        <property type="term" value="C:multi-eIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12278">
        <property type="entry name" value="RRM_eIF3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000235">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03001">
        <property type="entry name" value="eIF3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011400">
        <property type="entry name" value="EIF3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034363">
        <property type="entry name" value="eIF3B_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013979">
        <property type="entry name" value="TIF_beta_prop-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14068">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 EIF3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14068:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08662">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036424">
        <property type="entry name" value="eIF3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7 8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6 7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="37"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR036424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW29938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="710" mass="81094" checksum="07417754DB8139D3" modified="2011-11-16" version="1">MSLTEEEYLQLEKEINLDDIDFSDLEEKYEVDTGLDNYVVVDGAPIAPEAKVPVLIKVLKKLFSTVGEVVEGDDGIYMPLEGGKSKGYLFVQFKTSEMADAAIRQLHGKKLDQKHRLLVNKLSDIEKFGAEGAVPSEFKEPEVAPFHSHGYLKSWLQDKQGRDQMVLHFSDSVGVYWNKKKQDPEPVIEPRRAFTSKYAKFSPKGTYLFSIHPQGIQSWGGEDFKSIHRYIHNQVRLIDFSPNEKYMVTLSPVPITLPDRISDRASFPFGPESQGHKLVIWNLATGEPAKTFALPPHLEGQKEMPWPLVKWSHDDKYCARQGPDALAIYETPSFQLLDKKLVKIEGIQDFEWAPAGVPLHNSKTPGGEYVLSYWTPESTNQTARVSLMQVPSKEVLRTINLFQVSGCKMHWQNEGKLLCVKVDRHTKSGKTVFSNLEFFRTSERDIPVEKLELKEVVINFSWEPKSERFVTISRLDDGNPNPAIPKNTISFYAVESAKGGKARVFKNYKKIENKHSNSVYWSPKGRYVVVATISRSSGELEFFDVSFDDENKKNPANVKLLNTTKFSGMTNLSWDPSGRFVAAWSSSWLHSIENGYRLYEFTGSLLREQTIDQFREFIWRPRPESLLTSGDKKKVRANLKDLSAQFEVDDAMEADAALKEAILARKQALEEWRKYRAKHLGKKVQTNEVQAEIIEEIKEEIIEEKEEIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVN7</accession>
    <name>G3AVN7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Putative mitochondrial carrier protein PET8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_144171</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377969.1">
        <property type="nucleotide sequence ID" value="XM_007377907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVN7"/>
    <dbReference type="FunCoup" id="G3AVN7">
        <property type="interactions" value="761"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVN7"/>
    <dbReference type="GeneID" id="18870605"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144171"/>
    <dbReference type="eggNOG" id="KOG0768">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_3_0_1"/>
    <dbReference type="InParanoid" id="G3AVN7"/>
    <dbReference type="OMA" id="IGPRTMW"/>
    <dbReference type="OrthoDB" id="276989at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000095">
        <property type="term" value="F:S-adenosyl-L-methionine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000018">
        <property type="entry name" value="S-adenosylmethionine mitochondrial carrier protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45667">
        <property type="entry name" value="S-ADENOSYLMETHIONINE MITOCHONDRIAL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 14" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="4"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="87"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="186"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW30203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="279" mass="30363" checksum="5AEF67170D98D910" modified="2011-11-16" version="1">MDNSTFIISLISGACAGTSTDLAFFPIDTIKTRLQAKGGFFANGGYHGIYRGLGSCVVASAPSASLFFITYDSMKIYTKQYIDSPVQRHMISSSCGEIAACLVRVPAEVIKQRTQTGRVGHNGVSSSWSNFMYLIKDKSGEGLIRGLYRGWNTTIMREIPFTVIQFPLYEWLKEKWAKTTHSAEKLSAVKGAICGSIAGGVAAALTTPLDVIKTRIMLHKDRVNVVDVVKSIIREEGLGTFLNGIGPRTAWISAGGAIFLGCYELVHASLSYSKEEKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADV8</accession>
    <name>G3ADV8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Vacuolar protein sorting-associated protein 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_57730</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the retromer complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372094.1">
        <property type="nucleotide sequence ID" value="XM_007372032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ADV8"/>
    <dbReference type="FunCoup" id="G3ADV8">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ADV8"/>
    <dbReference type="GeneID" id="18872172"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57730"/>
    <dbReference type="eggNOG" id="KOG2273">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028982_1_0_1"/>
    <dbReference type="InParanoid" id="G3ADV8"/>
    <dbReference type="OMA" id="FYLGTME"/>
    <dbReference type="OrthoDB" id="9976382at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030905">
        <property type="term" value="C:retromer, tubulation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140318">
        <property type="term" value="F:protein transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06891">
        <property type="entry name" value="PX_Vps17p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014461">
        <property type="entry name" value="Retromer_complex_Vps17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053055">
        <property type="entry name" value="VPS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037907">
        <property type="entry name" value="Vps17_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015404">
        <property type="entry name" value="Vps5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47433">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47433:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09325">
        <property type="entry name" value="Vps5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011791">
        <property type="entry name" value="Vps17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="3">
        <location>
            <begin position="117"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Sorting nexin/Vps5-like C-terminal" evidence="4">
        <location>
            <begin position="249"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="477"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="487"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="506"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR011791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="536" mass="61666" checksum="AC8171C0060B65EE" modified="2011-11-16" version="1">MSFNPDEFENNNPFADPLAEQHQNQPAPVRDSTAPPIDYNNGVQPPTTEEENEGDNQESNLLNESGQLTEAEFRKLIPERFTNKYELKIKLIGVERNKPGNPILKMDAQVKGLPRFRQVKYKDIRRTFNEVVKFNKYLTISNLEVFVPVIPSANTSYPTNGEDETKQLVMVWQEWLDRITSNPILIRDEEFVYFIENDFGYSVINSNRKSSVASGLMRHTLKQFSVPYDPYVELADFRPMIKNAYLICQKLHKILDRHSKTEKQLSIHIFDMSNKLNVLSQFENTHPGMKNMWEKLGKITQIQSDLTLIESINDMGTLGDGVQALIDDFYEIKEALTNRHLIMRELLQAEAQTAAKHAQANKIKSRSSLDPIKVDEALRSLEYATKVEESLNLQVKRISGEMLYERKEVIDFTEKKLQKLVKSFTLHKVEHHRKILKHLETIRLDIRIVDERGGLSRLNRDNLGNLKHNLVQSQSAKGDSWSSRTFRSLEKEEQERENQNDLGESDSSKPFDDDNTNKSSVVDAKNAASLLGIATF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE13</accession>
    <name>G3AE13_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADPH-dependent methylglyoxal reductase GRE2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_69753</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372959.1">
        <property type="nucleotide sequence ID" value="XM_007372897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE13"/>
    <dbReference type="SMR" id="G3AE13"/>
    <dbReference type="STRING" id="619300.G3AE13"/>
    <dbReference type="GeneID" id="18875287"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_69753"/>
    <dbReference type="eggNOG" id="KOG1502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_9_2_1"/>
    <dbReference type="InParanoid" id="G3AE13"/>
    <dbReference type="OMA" id="CMERKLW"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05227">
        <property type="entry name" value="AR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="336" mass="37167" checksum="DEC06CEFBCEC816D" modified="2011-11-16" version="1">MLSTTVFVSGANGFIAQHIVKQLISKNYKVLGSVRAATKGEELAANLNSPNFSYVVVPDIGVAGAFDDALKSHPEVIIFLHTASPFRYDIQDIEKELLNPAVNGTKNALKSVQEYAPQVTNVVVTSSFAAIGWSNKYFDPTKVSTEEDWNPQTWQDALANPDDGYLGSKKFAEQAAWEFVKIEQPNFRLTTVNPPVAFGPQAFPIKNKKELNTSAEVINGLLKLQQGYDIPEVRHLFVDVRDVAAAHISAFENPDAAGKRLFVSSGPWSAQGIVNIFHKNFPQLSYIPIGEPWKDTLYRSSTFKFDNSKTVEILKVDLRPLEVSIVDAVQQILEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE57</accession>
    <name>G3AE57_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_131311</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373003.1">
        <property type="nucleotide sequence ID" value="XM_007372941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE57"/>
    <dbReference type="GeneID" id="18869563"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131311"/>
    <dbReference type="eggNOG" id="ENOG502S9IG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1219559_0_0_1"/>
    <dbReference type="InParanoid" id="G3AE57"/>
    <dbReference type="OMA" id="SYETIYW"/>
    <dbReference type="OrthoDB" id="4041975at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="220" mass="25923" checksum="E979895B9D7572D9" modified="2011-11-16" version="1">MTTVIELLYEFDQFLTTQLLKNSFTADLLATPASKFITELLVIVSIGLISYETIYWSGIYLNLWEYHAKDIFTEIPIHCAHVHIRLNVIKKSDQDKLKEYYELKQKSKYNVLYWNRLTQLSTEIFLLEKFVKYYFEFSPEDFEMNEEPELGSTIEHLRNKTLNIFKESDAYKHLDNHSITIDDVVIYNNKNQLVPKAENNNYLSKCQIETGNVIDSVILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE76</accession>
    <name>G3AE76_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Translation machinery-associated protein 20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_58828</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 6">Involved in translation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 6">Belongs to the TMA20 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373022.1">
        <property type="nucleotide sequence ID" value="XM_007372960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE76"/>
    <dbReference type="FunCoup" id="G3AE76">
        <property type="interactions" value="545"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AE76"/>
    <dbReference type="GeneID" id="18872630"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58828"/>
    <dbReference type="eggNOG" id="KOG2523">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090468_0_1_1"/>
    <dbReference type="InParanoid" id="G3AE76"/>
    <dbReference type="OMA" id="GVENIHY"/>
    <dbReference type="OrthoDB" id="10249667at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11609">
        <property type="entry name" value="MCT1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21155">
        <property type="entry name" value="PUA_MCTS-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.400.20:FF:000001">
        <property type="entry name" value="Malignant T-cell-amplified sequence 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016437">
        <property type="entry name" value="MCT-1/Tma20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041366">
        <property type="entry name" value="Pre-PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798:SF0">
        <property type="entry name" value="MALIGNANT T-CELL-AMPLIFIED SEQUENCE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798">
        <property type="entry name" value="MCT-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17832">
        <property type="entry name" value="Pre-PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005067">
        <property type="entry name" value="Tma_RNA-bind_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="7">
        <location>
            <begin position="92"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00060251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00070056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR005067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="180" mass="20249" checksum="34F08AD11853FCD7" modified="2011-11-16" version="1">MFKKFTKEDIHSRSNIKSSVQRGLKSNLVQLYPQLEPAIDNLIPKKSQVVSIKCEEKIQLYCVDNEIVMFQQYNDELVPSLTTVHKYPDCFPRVQVDRGAIKFILSGANIMCPGLTSPGAKLPEENMEKDAIVTVYAEGKENALAIGKLLMSIDDIKTKNKGIGIELVHYLGDGLWNHQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE99</accession>
    <name>G3AE99_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_147087</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373045.1">
        <property type="nucleotide sequence ID" value="XM_007372983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE99"/>
    <dbReference type="FunCoup" id="G3AE99">
        <property type="interactions" value="1101"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AE99"/>
    <dbReference type="GeneID" id="18870740"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_147087"/>
    <dbReference type="eggNOG" id="KOG1092">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018687_4_1_1"/>
    <dbReference type="InParanoid" id="G3AE99"/>
    <dbReference type="OMA" id="EFHVFVC"/>
    <dbReference type="OrthoDB" id="26371at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000007">
        <property type="entry name" value="TBC1 domain family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000001">
        <property type="entry name" value="TBC1 domain family member 22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF26">
        <property type="entry name" value="LD44506P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="261"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="627" mass="71463" checksum="7F42B488775CB7F8" modified="2011-11-16" version="1">MGKKKQEMAQVYNGTSNEFIFGEQGGKGKNLSSFLKNLSLTGSSTLSHSSSARDSVEETNSQIYGSLGYANSTSSLNSSGNLFNPKRPTNTQRTVSVGSGLPHTSTGLGSGFPERMTPVSGGSGGKISPYSSHKQSQPRRSRSMSRYTDLDDDWDADLQEVSSPKKELTKLDTEESSRTSLNAQTSPMLDSLYPDLMLPSGNGVSAASKPPKESLDFEDMQTKMEINKLNLLNSKYAKFRKVLTSDNNINLQDLRKLAWNGIPNELRAVSWLLLLGYLPTNKSRQSSTLKRKRQEYLEGLDSVTIEFHDDPPDNESTTSLSNANRDKMLYHQIKIDVKRTNPTLKLYSYTATQMSLRKILYLWAVRHPASGYVQGINDLCTPFYQIFLNNYIWQLQRKQQLLQRQHKSNNSSTGSLPEDDDMDLYIPGYLASEEEDDPEELKLLQDPQLPQYTLDNFDTSWLSPRITAIIEADTYWCLSRLLENITDNYIHEQPGIIRQVNELRNLISKIDHELIKHFDSEGVEFIQFSFRWMNCLLMRELPIQLIIRMWDTYLSETPLGFNTFHTYVCAAFLIKFSGDLKAKDFQEILLFLQNPPTSRWKEKDVELMLSEAFIWQSLYKNASAHLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEA6</accession>
    <name>G3AEA6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_131772</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373052.1">
        <property type="nucleotide sequence ID" value="XM_007372990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEA6"/>
    <dbReference type="STRING" id="619300.G3AEA6"/>
    <dbReference type="GeneID" id="18869608"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131772"/>
    <dbReference type="eggNOG" id="ENOG502RQ2X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075125_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEA6"/>
    <dbReference type="OMA" id="HPRYYIT"/>
    <dbReference type="OrthoDB" id="4025944at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="252" mass="29573" checksum="8051C27EF90E442B" modified="2011-11-16" version="1">MLRFKALRVYTLPPRSSYIINKTYPLVLWNNLIPYKFKFFTGLGTVALSIKSLHPNTVIACLPPLTIGYYFGYKRWRHWLYLNNSTRAEQSGVVRFERYDETKVENVLSGIENEYDCLTRQIVDVVEQRVIEHVAETSDDVSELFVHDDQFSMHIFPTEVETWITSEVDVDGELVKFIKMSMPFYSVRDVETRKRLGVVTVYLRQVAKSEHEDFHMHIEVSPLTWFKPKVLVINDVPDVLNSKLFEERNKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEG5</accession>
    <name>G3AEG5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">FACT complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_48718</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the FACT complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the peptidase M24 family. SPT16 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373165.1">
        <property type="nucleotide sequence ID" value="XM_007373103.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEG5"/>
    <dbReference type="FunCoup" id="G3AEG5">
        <property type="interactions" value="1390"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEG5"/>
    <dbReference type="GeneID" id="18871257"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48718"/>
    <dbReference type="eggNOG" id="KOG1189">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004627_1_0_1"/>
    <dbReference type="InParanoid" id="G3AEG5"/>
    <dbReference type="OMA" id="YHINTIP"/>
    <dbReference type="OrthoDB" id="10251642at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140713">
        <property type="term" value="F:histone chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140719">
        <property type="term" value="P:constitutive heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045899">
        <property type="term" value="P:positive regulation of RNA polymerase II transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007063">
        <property type="term" value="P:regulation of sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01091">
        <property type="entry name" value="CDC68-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.150:FF:000002">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.210:FF:000001">
        <property type="entry name" value="FACT complex subunit spt16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000017">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000005">
        <property type="entry name" value="FACT complex subunit spt16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.210">
        <property type="entry name" value="FACT complex subunit Spt16p/Cdc68p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029148">
        <property type="entry name" value="FACT-SPT16_Nlobe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056595">
        <property type="entry name" value="Fact-SPT16_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048969">
        <property type="entry name" value="FACT_SPT16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013953">
        <property type="entry name" value="FACT_SPT16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040258">
        <property type="entry name" value="Spt16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033825">
        <property type="entry name" value="Spt16_M24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980">
        <property type="entry name" value="CDC68 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980:SF15">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14826">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24824">
        <property type="entry name" value="PH_SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08644">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21091">
        <property type="entry name" value="SPT16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01285">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01286">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FACT complex subunit SPT16 N-terminal lobe" evidence="12">
        <location>
            <begin position="6"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="FACT complex subunit SPT16 middle" evidence="13">
        <location>
            <begin position="541"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="14">
        <location>
            <begin position="817"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="936"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="940"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="975"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="985"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1000"/>
            <end position="1013"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW35753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1013" mass="115666" checksum="01D260A6BC438D6A" modified="2011-11-16" version="1">MTDVKIDAGLFHKRLSILQKNIVAENITQALVIVGAREDENTYKKSTVLQNWLLGYEFVHTAIYVTKDKCVFLTSEGKAKYLKGLTGKPSSNSSEVEIWVRTKDADKNHQLFVDLIKTLKENGTSYGSVLKDKYQGKLLDEWKKVLEEEGGDKLTPVDIAILISKSMEVKDSEEFNNTKIASKASVVMMDTFADEMMVVVDEEKKVTNSQISDQIEDKIENNKWYLKSKLGKSLLQPLKEFDPEFLEWCYSPIVQSGGEYDLKPSAVSTDAMLIGDGVILSSIGLRYKAYCSNVARTFLIDPTPEIEANYDFLLKLQNHITSTLLKDGVQANKLYQGAVDYIKKEKPDLVSHFTKNAGWLLGIEFRDSTFVLNAKNERSLLNGQIISLTIGFNNLSNSSAKNPKLKTYSLLLTDTFKVSESEPILLTTYPKSKSEISFYFKDENEDKKLKTESIKNEGAELNSKILKSKLRHETNEVDDANAEKVRQGIQSKLHEKRLQEGLARFSKADATDSGDFKPVFKKYESYIRESQIPANVRDLRIHIDIKNQTIILPIQGRPVPFHINSYKSGSQTEEGDFTSLRLNFNSPGAGGNVSKKIELPYEDSPDNTFLRSITLRSRDRQRMIDVYKAIQDMKKESVKRESEKKQMADVVTQANLIELKGTRMKKLEQVFVRPTPDTKKLGGVLQIHENGLRYQSSFKSDQKVDVLFSNIKHLFFQPCKDELIVIIHCHLKNPIMIGKKKTFDVQFYREASDMAFDETGGRKRRYRYGDEDELQQEQEERRRKTLLDKEFKAFAELIADSSHGLIDLDIPFRELGFQGVPFRSSVFCMPTRDCLVQLVDPPYLVVTLEEIEIAHLERVQFGLKNFDLVFVFKDFNKPVVHVNTIPMEVLEDVKSWLTDVDIPISEGQMNLNWGTIMKTVQSDPYQFFVDGGWSFLTGEGDSDEEDEEEEESEFEVSDEDPQDEDEDEESEASDDYSASGSDSEGSFDEESDDGEDWDEMEKKAAREDKRFRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEY5</accession>
    <name>G3AEY5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">NADH-ubiquinone oxidoreductase B14 subunit (CI-B14)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_57861</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I LYR family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372201.1">
        <property type="nucleotide sequence ID" value="XM_007372139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEY5"/>
    <dbReference type="STRING" id="619300.G3AEY5"/>
    <dbReference type="GeneID" id="18872224"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57861"/>
    <dbReference type="eggNOG" id="KOG3426">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111660_0_1_1"/>
    <dbReference type="InParanoid" id="G3AEY5"/>
    <dbReference type="OMA" id="FWKQTTH"/>
    <dbReference type="OrthoDB" id="14535at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20266">
        <property type="entry name" value="Complex1_LYR_NDUFA6_LYRM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045299">
        <property type="entry name" value="Complex1_LYR_NDUFA6_LYRM6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016488">
        <property type="entry name" value="NADH_Ub_cplx-1_asu_su-6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12964:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12964">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B14 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006643">
        <property type="entry name" value="NDUA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="9">
        <location>
            <begin position="22"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="121" mass="14687" checksum="7FFDABB575FF758F" modified="2011-11-16" version="1">MTLATEFAETTHRSLTNAELRKRVLHLYRKYMRHSKDFSDLYELDMPISNIKTKIRQEFERQRFLNDVNVANVVYAKGQMEFQELINFWKQQCHVLRYFDEQRAYGVVDKNDFVKNFLRGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF23</accession>
    <name>G3AF23_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ATP-dependent ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_47902</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372239.1">
        <property type="nucleotide sequence ID" value="XM_007372177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF23"/>
    <dbReference type="FunCoup" id="G3AF23">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF23"/>
    <dbReference type="GeneID" id="18871158"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_47902"/>
    <dbReference type="eggNOG" id="KOG0065">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_57_4_1"/>
    <dbReference type="InParanoid" id="G3AF23"/>
    <dbReference type="OMA" id="AYFWYAF"/>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF119">
        <property type="entry name" value="ROA1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="423"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="454"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="489"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="571"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1067"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1091"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1124"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1203"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="1"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="647"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="604"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1230" mass="138495" checksum="C6794588F7473880" modified="2011-11-16" version="1">METSGELDQSHKILNDISFELKSGSMMAIMGGSGSGKTTLLNTLSQRTNINNKKLLFTGSITYTRQENLSHISHAYMLQNDLFLPGLTVFETLKSQADLRLPSRITHVEKLQLIDYILGVLGIAHVKNTRVADFSSHSSSLSGGEQRRVSLAVQLLSRPSILFLDEPTTGLDSSTSLKLITLLQQLSSEFGMSIVVSIHQPRSEIMKLFDEICLLTRGGRMVYFGNVDHAQTYFDGLDFLEGSSADYFNHIEYIMGLSVKDTTSREREHQTIARIDKLVKMWKENHEEYQDDDKKPDSFEDNLKLFIRGKEDRISFWKELIILTKRTFVLSYRDIQGLLVLNFGAIIVSVTLGWMFFQPKHDLAGIRSLTSSLYVMLEVVAFCPMYYEIERLWLTDGVFFYREYSENWVTISGFMISRRLGKFLLEDLPMSIIFAVVSYFMWGLGFSNGSNFGVYFALVLIIELCCMASSMLCFALAPTLPISALIVNIFYQLQNSACGFFVNAATMPVYVRWTKYIAHFWYAFGALASNQFSGWVGECPFEEGDVRCVSYTGDYQLELLGFPRGWIGEPIGILICWFVGYLFLAGIAIYFKRFDVRMAKTRENKFEDEQQEEEERNKEISGGSSVIEPQSPPSTPTNEVNNDDFQIDIENIHLSVKKTRFNKQAKCILDGINATFHANAVNVIMGPSGSGKTTLLNYLSNRLPKSSSYINSGSIKVNHHQTITQEELAKIGAYVTQHDTSLIDNLTVRETLYYQAKIRLPLKEHKNIHTIINSLIRHTGLVDCADTVIGSDFVKGISGGEKRRVSIAIQLLSKPKVLFLDEPTSGLDSSTAETIYSLLETLAKENGTTVILTIHQPSYEMFCTFGSLLLLGYGGKVIYQGDSIGVIKYLSRLGYPMKKDTNVADFVLDLVSKREEGEGSITDYLSNNWNNNEKERIITYCSSENLDIRQHFHKRLPFRVTFPTIVERQVVTSYRSPDVVFSRTGQIILLAIVHTLYFAPLRNTQDGISNRLGLIQEVLNLYFVGLVNNISLYPFERNLFYQEYKDGVYGVSEFGISYFLNELPTEIIPSLFFAALIVFACGLPRTVNMFFAMYGTAALSINCGESLGIVVNSCFNHMGVATNVLTVFVIIAIFMGGTMSLHMPSFFKGINYISPMKYAVAICANLGLRGQVFECSTGLDECVFETGEAVLEYYNLKADLGPMVGGLIACFVLYRVIAIASIYVRVKWFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFD6</accession>
    <name>G3AFD6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GTP-binding protein ypt1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_133146</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Lipid-anchor</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372337.1">
        <property type="nucleotide sequence ID" value="XM_007372275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFD6"/>
    <dbReference type="FunCoup" id="G3AFD6">
        <property type="interactions" value="1150"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFD6"/>
    <dbReference type="GeneID" id="18869725"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133146"/>
    <dbReference type="eggNOG" id="KOG0084">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_23_1_1"/>
    <dbReference type="InParanoid" id="G3AFD6"/>
    <dbReference type="OMA" id="TQMAKDF"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090114">
        <property type="term" value="P:COPII-coated vesicle budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048194">
        <property type="term" value="P:Golgi vesicle budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048211">
        <property type="term" value="P:Golgi vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990261">
        <property type="term" value="P:pre-mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900101">
        <property type="term" value="P:regulation of endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035493">
        <property type="term" value="P:SNARE complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035494">
        <property type="term" value="P:SNARE complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01869">
        <property type="entry name" value="Rab1_Ypt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000359">
        <property type="entry name" value="Small GTP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057289">
        <property type="entry name" value="Rab1/Ypt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="174"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="23024" checksum="66DD6F7CF6E9A84E" modified="2011-11-16" version="1">MNNEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTPDYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLQEIDRYATGGVMKLLVGNKADLSDKKIVEYTAAKEFADALDIPFLETSALSSTNVEQAFYTMARQIKAQMTSNTNSGNAGNAKGKSNVNLRGESLTSNQSNSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFJ0</accession>
    <name>G3AFJ0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Globin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_64181</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372391.1">
        <property type="nucleotide sequence ID" value="XM_007372329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFJ0"/>
    <dbReference type="GeneID" id="18874960"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64181"/>
    <dbReference type="eggNOG" id="KOG3378">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_950501_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFJ0"/>
    <dbReference type="OrthoDB" id="436496at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008941">
        <property type="term" value="F:nitric oxide dioxygenase NAD(P)H activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071500">
        <property type="term" value="P:cellular response to nitrosative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046210">
        <property type="term" value="P:nitric oxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01040">
        <property type="entry name" value="Mb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044399">
        <property type="entry name" value="Mb-like_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396:SF6">
        <property type="entry name" value="ABL201WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396">
        <property type="entry name" value="FLAVOHEMOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Globin" evidence="2">
        <location>
            <begin position="74"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="293" mass="33583" checksum="F39BC21E168BA00E" modified="2011-11-16" version="1">MITEDSHTSEENQIKITLNLSESEIEIIRYTWNKMFLEDEGIATRPLIRCTSRDFPGGYPEEGANPVQDVSSTTSSIAQMLEAKRLATVTGVFCYQVYQNLSARYPGLEKMFPSLKHQATHFATVLRITVTQLEDLSQLDDYLCRLGKRHSRILKVEPLPYELMGEALIETFQQRFGTKFNQDFETAWTKLYLFLANSILQFGVDPVMTCEGPLHNNRVYTQPTSTDDESSSQESISFKENMEPSPTIDLEEIIPERGRKSPYDLYKRPTIKYSRSSMAAMATAAIKRKYRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFV0</accession>
    <name>G3AFV0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58234</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372501.1">
        <property type="nucleotide sequence ID" value="XM_007372439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFV0"/>
    <dbReference type="FunCoup" id="G3AFV0">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="GeneID" id="18872374"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58234"/>
    <dbReference type="eggNOG" id="ENOG502RZCP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051372_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFV0"/>
    <dbReference type="OrthoDB" id="4083656at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="265" mass="30426" checksum="A4769AB8388FE2C5" modified="2011-11-16" version="1" fragment="single">MTLLLPRIGLQSLTRGGLLSSRVPRISSCLVRFKSEVPSPYNPKKDLPTAGEWNYLKQHIPGETVQTNTLKDRVPKFPLGKENVPTLLPRPGVPQVGSKYTFTQVINILENKTKPELIYESEPHRLYFLTCLACAVVFAVYACVLLEWAFYMARTEYQENEELIVNEQLRKRQWVFSLMTYMIPSTIMFGFAYFIGLFPTRLVRRMWYLPGANPHIQFTSYPLLPGKPTPVYTVPLANLSRKHTAKIWTGKGFYGTADKGFFFFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFX1</accession>
    <name>G3AFX1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58251</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372522.1">
        <property type="nucleotide sequence ID" value="XM_007372460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFX1"/>
    <dbReference type="GeneID" id="18872387"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58251"/>
    <dbReference type="HOGENOM" id="CLU_2943289_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFX1"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="60" mass="6956" checksum="39CC4C1BE282DBFE" modified="2011-11-16" version="1">MPYGMSAAWPHGTIFIESSYITTTYVRFLIYELSFSCYFSTASSESLKIQKNVKKIDKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGB4</accession>
    <name>G3AGB4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1</fullName>
            <shortName evidence="8">Oligosaccharyl transferase subunit WBP1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_58463</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the DDOST 48 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372665.1">
        <property type="nucleotide sequence ID" value="XM_007372603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGB4"/>
    <dbReference type="FunCoup" id="G3AGB4">
        <property type="interactions" value="1075"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGB4"/>
    <dbReference type="GeneID" id="18872461"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58463"/>
    <dbReference type="eggNOG" id="KOG2754">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031804_1_0_1"/>
    <dbReference type="InParanoid" id="G3AGB4"/>
    <dbReference type="OMA" id="NIATECG"/>
    <dbReference type="OrthoDB" id="29105at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004576">
        <property type="term" value="F:oligosaccharyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005013">
        <property type="entry name" value="DDOST_48_kDa_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055459">
        <property type="entry name" value="OST48_MD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055457">
        <property type="entry name" value="OST48_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10830">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE 48 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10830:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE 48 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23358">
        <property type="entry name" value="OST48_MD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03345">
        <property type="entry name" value="OST48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="OST48 N-terminal" evidence="9">
        <location>
            <begin position="2"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="OST48 middle" evidence="10">
        <location>
            <begin position="171"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW35253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="332" mass="37316" checksum="BCDE654CA51AF5E4" modified="2011-11-16" version="1">MKFVNHFGGNVISIGGEKSVLPNGIRSFLNELGIYPSPKNFNYLDHFNTKDGSVVLSSENNLVPGNRLVESLDNVKYHGNGALISNNELIFPIVKSSSTGYTGKAGEQVDSSTAWTLGEQGFLAVGLQALNNARVAWLGSSELLQFEHIYKWAFQKQGVLKLQYVEHYNTKNETAQNPHLYRINDDVVYTIGVSESVDGEWVPYQIASDDDQLQVSFKLLDPYQRLNLVSVGPSDSPDGPATAQTYRVNFTVPDHHGVFTFELDYKRVGLSYLQDKRVVTVRHLANDEFKRSWDITNSWLYIVSVAIVIAAWFLFVISYIYVGNTNYQKKNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGG8</accession>
    <name>G3AGG8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58530</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372719.1">
        <property type="nucleotide sequence ID" value="XM_007372657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGG8"/>
    <dbReference type="GeneID" id="18872489"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58530"/>
    <dbReference type="HOGENOM" id="CLU_2361063_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGG8"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="96" mass="11223" checksum="89ACE08485AB0C3A" modified="2011-11-16" version="1">MQEVWLCIISFGYVAVLRCCALCRNFSIVVWKNELHDKSELTSLPPSVLNYYGNIYINVDLMKLHLMCNLIDQKLSYLIPIAYDRLIRASNSKLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGH9</accession>
    <name>G3AGH9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Replication termination factor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58542</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372730.1">
        <property type="nucleotide sequence ID" value="XM_007372668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGH9"/>
    <dbReference type="STRING" id="619300.G3AGH9"/>
    <dbReference type="GeneID" id="18872498"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58542"/>
    <dbReference type="eggNOG" id="KOG3113">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048955_2_1_1"/>
    <dbReference type="InParanoid" id="G3AGH9"/>
    <dbReference type="OMA" id="EFRWLHC"/>
    <dbReference type="OrthoDB" id="247013at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006274">
        <property type="term" value="P:DNA replication termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006735">
        <property type="entry name" value="Rtf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12775">
        <property type="entry name" value="PROTEIN C20ORF43 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12775:SF0">
        <property type="entry name" value="REPLICATION TERMINATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04641">
        <property type="entry name" value="Rtf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="25311" checksum="F564F37C40164CB1" modified="2011-11-16" version="1">MGADGGTIAKRKDLINVHSSLDKKNSTGIGYDNELTLLNSCGISAFPLHTKSNDPIVGDYKGRLYLKEKILQYLIDSKQSKSRVNPKLSHITSLKDICDVKIEWDTSDEPRIRCPITKISSAKSTYAYLRPCGCLMSSKVITELNQNNSKGKCPNCDATFSSDCDVVIVNPLGKEEYNKLNESSYKILCNQQLSHSKKPIKSKKVERKQSIKRAADSQEPVSKRIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGK3</accession>
    <name>G3AGK3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_69609</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372754.1">
        <property type="nucleotide sequence ID" value="XM_007372692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGK3"/>
    <dbReference type="STRING" id="619300.G3AGK3"/>
    <dbReference type="GeneID" id="18875278"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_69609"/>
    <dbReference type="eggNOG" id="ENOG502RS0Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012462_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGK3"/>
    <dbReference type="OMA" id="GLNKYFH"/>
    <dbReference type="OrthoDB" id="4082726at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021861">
        <property type="entry name" value="THO_THOC1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11957">
        <property type="entry name" value="efThoc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="600"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="609"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="635" mass="74451" checksum="2534A4F6BFAF199C" modified="2011-11-16" version="1">MSSKLQIPIDSICAIIDHVVSRITDDTDKNYIFEQVDDILFSEDAKKIQTTLDEAQFDYKLEVDQEISEEDKAKINEFNESLDSKSKEFVISTSFQSTINGILGTYAEINNDLDKSNFYNKLAILLDFEIELFTDVYTHTKYIYYNSLSQISKFLVSVGTAEIEYFWSYLETRELLFQTKIFDRGSIGDRIAVLEICNYLTDRLTRKDSAGKRNSYKKDTFNDRFQYRVRVFVTNTFAFEDNTGLNKYFHTADRQAPELPSRNKFLEDIISVNKIFNDPYYFAKKANTKELSTMADRMVGICKYLTGEELDYAASVGQTDQYQIPEPKSEEEQQYLSEKYSDLFYVPETYLMSSFGYKDRKEHEKLQVEDREFLDKQFETSKVRLQYLVQILFVVSFLIELAPRNKADFMASLGAPTHVKHLTDEFISESNRTALWKIKRDLFASLKSIDGQLSFVLSHLLLSERAWWGWLIYGKDPNTNKSYFYDRILDQEELDKVEEDRTKLFPYKSKKYFNSYVTPQLTRRMRVERGIGRLHSLTKFDSVFSKAKVEQLSDSIAECEDSPERKEMLEERSILLWKCLRKERSSNWLKFGEILRPEMLQSEPATNGEVEKESDRAKRPLETDESDNVPKRVKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGW5</accession>
    <name>G3AGW5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Vacuolar protein sorting-associated protein 29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_57688</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the VPS29 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372050.1">
        <property type="nucleotide sequence ID" value="XM_007371988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGW5"/>
    <dbReference type="FunCoup" id="G3AGW5">
        <property type="interactions" value="1080"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGW5"/>
    <dbReference type="GeneID" id="18872153"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57688"/>
    <dbReference type="eggNOG" id="KOG3325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063749_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGW5"/>
    <dbReference type="OMA" id="IHGHQCI"/>
    <dbReference type="OrthoDB" id="10258130at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170071">
        <property type="term" value="C:CROP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030906">
        <property type="term" value="C:retromer, cargo-selective complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140312">
        <property type="term" value="F:cargo adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11124">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN VPS29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="5">
        <location>
            <begin position="39"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="165"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="168"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="266" mass="30079" checksum="553B412454E0E2ED" modified="2011-11-16" version="1">MLTLAIGDLFIPERSIDLPTKFKKLLAPNPNSIPSNNKISRVVCLGNILNSQESLKFLHNLSPQFNLVKGEYDNSTILSQQLSILSHNKEEIIPYCNVFTHDNLRIGYTNGYQIVPKGDPLALSAFSRELDVDILIWGGSHRVEAYTLDGKFFVNPGSATGAITFDWPDNEAYEEEEEANKETEMKEDKPDEKKEEKETEQGIEDQTESKQESSKINKEILSEVTELNSNIPSFCLLDIQGSTCILYIYTYLNGEVKVDKVTYTRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH19</accession>
    <name>G3AH19_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Alpha-mannosidase</fullName>
            <ecNumber evidence="3">3.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_48441</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Degrades free oligosaccharides in the vacuole.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.</text>
            <dbReference type="EC" id="3.2.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.24" evidence="3"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372861.1">
        <property type="nucleotide sequence ID" value="XM_007372799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH19"/>
    <dbReference type="FunCoup" id="G3AH19">
        <property type="interactions" value="262"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AH19"/>
    <dbReference type="GeneID" id="18871220"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48441"/>
    <dbReference type="eggNOG" id="KOG4342">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003442_0_1_1"/>
    <dbReference type="InParanoid" id="G3AH19"/>
    <dbReference type="OMA" id="GQYWDAW"/>
    <dbReference type="OrthoDB" id="10261055at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034270">
        <property type="term" value="C:Cvt complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019309">
        <property type="term" value="P:mannose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009313">
        <property type="term" value="P:oligosaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000004">
        <property type="entry name" value="alpha-mannosidase 2C1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.110.10:FF:000002">
        <property type="entry name" value="alpha-mannosidase 2C1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000001">
        <property type="entry name" value="alpha-mannosidase 2C1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054723">
        <property type="entry name" value="Ams1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041147">
        <property type="entry name" value="GH38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46017">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46017:SF1">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22907">
        <property type="entry name" value="Ams1-like_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17677">
        <property type="entry name" value="Glyco_hydro38C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="9">
        <location>
            <begin position="546"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1101" mass="126254" checksum="C5C453733FF40EF6" modified="2011-11-16" version="1">MGYDSFNRQPRFKPIDHLYESRLRQFTDGGRYVDLNLPKFYDVYRQEIGNLKSWKVPDNEHGKTQRPLWKDINFDEVHWENIGIGYRFGPSWKTFWVKFELDIPEEFLQSEGLEIQWETGSEGLLYNAVGLPLQAFSNERQSFRIPKEYRKLGKQLFYLEVACNGMFGNGSDGFPDPNKYFRLSRAHLVVLNMDARRLYYDFWILSDGSREFQGGTWQKYQAADLCTQIMNVFDSQDSESIKACRELARKMLGNKIDTEEVYDVFPHQDKRIDVFAVGNCHIDTAWEWPFAETKRKIVRSWTTQLKIADEYPEYVFVASQMQQFKWLKQYHPEILTRIKEKFTTNQFLPIGGSWVENDTNVPNGESLIRQFLLGQRFQMNEFGFYSNIFWLPDTFGYSSQIPQICQIVGVSRFLTQKLSWNNINTFPLTTFNWKALDGSQVLVHMPPANTYTASAHFGDVMRSSHQHKNLRDVPAGLLLYGHGDGGGGPTEEMLEKLRRCRGLANTVGLMPTVQLGVTIDDFYEHVLENSNQGQKLPTWTGEIYLEFHRGTYTTQALVKKYMRQGEIALHDLELIAGLVSIKFDKYKYPAKEINDLWEDLLLCQFHDVLPGSCIGEVYYDEVHPMLESLLKKAAGLIQDALRYLRKPKSVKEVQLVNTFPWDRTNEIVHVHKQESPELFQLLEGQNVGIVQNDGATRSISVNSINGETTINNSVDYPASVQEKDGFFVLSNKLLSAKISPTGIVTSLYDEVNQREIIDSTPTDQTSSEFIGGNQFVLFDDEPLNWPAWDTELHSLEKFKLLNNGESQVLVNDELESSILVTHRISSQSSIHTVISLAGVKTKDNLENNFLKFSSKVSWHENNLFLKVQFPTTIFTPPTANYETQFGITTRPTHYNTTWDVAKFEVAHHKFMDLSEFNYGVSILNNCKYGGAIHGNLMRLSLLRSAKAPDDRADMGDYHEFEYAIYPHAGNLGESTVRAGYNFNYKLIAEPIAHTDLFKSVKLEGGKSLVLSHIKRGENDYDVNQFEVFKTDTPEKSLVLRVYESLGGSSSGKLVFDKSLKVDSVFKTCALEIHGEQLRVDDGSVDISLRSFEIATYKVYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH42</accession>
    <name>G3AH42_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Brix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_131696</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372884.1">
        <property type="nucleotide sequence ID" value="XM_007372822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH42"/>
    <dbReference type="FunCoup" id="G3AH42">
        <property type="interactions" value="944"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AH42"/>
    <dbReference type="GeneID" id="18869600"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131696"/>
    <dbReference type="eggNOG" id="KOG2780">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040063_3_0_1"/>
    <dbReference type="InParanoid" id="G3AH42"/>
    <dbReference type="OMA" id="AWIISNK"/>
    <dbReference type="OrthoDB" id="264354at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10480:FF:000002">
        <property type="entry name" value="Ribosome production factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10480">
        <property type="entry name" value="Probable brix-domain ribosomal biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044281">
        <property type="entry name" value="IMP4/RPF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734:SF3">
        <property type="entry name" value="RIBOSOME PRODUCTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="2">
        <location>
            <begin position="96"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="298" mass="35063" checksum="914D2B37D944AFBB" modified="2011-11-16" version="1">MSTSTSEALKNIKNKQRRQKVFGEIKHEKNKARHKLRAERAKEERANPALREERIAQNIPDTLDNKRVFDETIGVEVEGEDQDEFQQYFTNLGSEPKILLTTSENAKKPAYEFADMLMDFLPNTTFIKRKKEYSMKEIAKFASNREYTDLLVINEDKKKVTGITFIHLPEGPTFYFSITSIVDGKRIKGHGRATDHIPELVLNNFNSRLGKTVGRLFQSIFPHQPEIQGRQVITLHNQRDYIFVRRHRYIFRNEEKVGLQELGPQFTLKLRRLQKGVRGDVIWEHRPDMERDKKKFYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHD3</accession>
    <name>G3AHD3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_49170</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373681.1">
        <property type="nucleotide sequence ID" value="XM_007373619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHD3"/>
    <dbReference type="FunCoup" id="G3AHD3">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="GeneID" id="18871311"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49170"/>
    <dbReference type="eggNOG" id="ENOG502RNI7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_525788_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHD3"/>
    <dbReference type="OMA" id="AKYECLA"/>
    <dbReference type="OrthoDB" id="4021219at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="343"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="481" mass="54317" checksum="9DC0D3856AB35C55" modified="2011-11-16" version="1">MTEIDSPYHDPIISLVQDPNLNKDNDKSSQLIKLIQNITERKVSKLCSSAFIILQTLEKLQMKFKSWEFLSLDYNSDYHFENKDDHIKQFNTGVADKVIVACSEMNIKIVKISADVDYISKSSRTLTPLDLISDSGTMLTSLVLRIIKLKNEITEHLTISYSKAKLISIGQELELMDEDESTVETYKSFIVSLLRQLNEAIESGDFEAKYECLAVINDMEQMFEKFKLERMMTDLSRTIEDNYSSDDEVQEPMSEIPSPPEDHSTDEDFFDYSDSDITNSVYSSQQLPLVHSITRQHTHQAKDSLSSWSQPKPTTITEELPYLMTAFSSAKAFEEDVSHYKSESSSSTTSSQTPISAPSSSRQIPTQETKARSAPPTASAEHRNKPFFHKAANLPDSSLYNESILVEKSLPSPYLNNSLLSRLGIRPQVVTVPMASTQPKGVLIDSKTANKNESNKLPFTKQNLLTQENLHSHDLENDLVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHR2</accession>
    <name>G3AHR2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59656</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373810.1">
        <property type="nucleotide sequence ID" value="XM_007373748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHR2"/>
    <dbReference type="GeneID" id="18873009"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59656"/>
    <dbReference type="eggNOG" id="ENOG502RZXU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1397133_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHR2"/>
    <dbReference type="OMA" id="GMHAFAV"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.20">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442409" evidence="1">
        <location>
            <begin position="19"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="195" mass="18491" checksum="D1A89036D0565DF1" modified="2011-11-16" version="1">MKVSSTLLVVAATTSVYALDLSDIKSAVTNAAADAQSHITSIAGEAKTAITSIAGEAKTDITSVAGNAASHITSAAAVAKTGITSVAGDAKSAITSVAENAKSHITSAAADAKSDITSAASVAKTGVTSVASDAKSGITSVASDARSVATSLASKAETAAADATSATSATTTTTTNGAAAVGVAGGMFALGLLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHU0</accession>
    <name>G3AHU0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_59679</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Substrate-binding subunit of tRNA (adenine-N1-)-methyltransferase, which catalyzes the formation of N1-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TRM6/GCD10 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373838.1">
        <property type="nucleotide sequence ID" value="XM_007373776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHU0"/>
    <dbReference type="FunCoup" id="G3AHU0">
        <property type="interactions" value="951"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHU0"/>
    <dbReference type="GeneID" id="18873021"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59679"/>
    <dbReference type="eggNOG" id="KOG1416">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010916_1_1_1"/>
    <dbReference type="InParanoid" id="G3AHU0"/>
    <dbReference type="OMA" id="TRCRPYQ"/>
    <dbReference type="OrthoDB" id="10254665at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160107">
        <property type="term" value="F:tRNA (adenine(58)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017423">
        <property type="entry name" value="TRM6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12945">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12945:SF0">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT TRM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04189">
        <property type="entry name" value="Gcd10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038170">
        <property type="entry name" value="tRNA_m1A_mtfrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR038170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="444" mass="50186" checksum="71122EFF718A43A8" modified="2011-11-16" version="1">MNTKNRTVITANQHVLIRLPSEGTKIVELRPSGVVSLGKFGSFLVDGVLGYPFGQSFEIVEGQDVKPILSLQHSSDPKDVSEEPEGDLNKEELTKMFSENNQNIIDIGSKIQKLTNDDIEELKKSGATSSIGQHIIDQMIAGHEGFDKKTLFSQQKYLRRKQQKFLRRFTVEYLGSSQMLQYYIDKDLPRVIDLSEESLGLLMTYGNVRPGGNYLLIDETGGVVLYAMLERMGGEGQVVLVHENEHVNIIALRHAGYSEEYINRMVKTIDYLQFLEPENEKINWEDKSEEELAEMKSSKREQYEKRRERATAINSVIETVQNGNFDALISVSTLHQPTLLPRILDTVGGSRPIVIYNQYKELLLETQHVLTKDKRVLAPSIFETRVRPYQTIPGRMHPLMTMRGGGGYILWGTKVIPQEGITAVGRGISKRKREESPATDATDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI12</accession>
    <name>G3AI12_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_59741</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373910.1">
        <property type="nucleotide sequence ID" value="XM_007373848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI12"/>
    <dbReference type="FunCoup" id="G3AI12">
        <property type="interactions" value="741"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI12"/>
    <dbReference type="GeneID" id="18873058"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59741"/>
    <dbReference type="eggNOG" id="KOG2485">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011106_0_1_1"/>
    <dbReference type="InParanoid" id="G3AI12"/>
    <dbReference type="OMA" id="GVLWPKF"/>
    <dbReference type="OrthoDB" id="269151at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01856">
        <property type="entry name" value="YlqF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45782">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45782:SF4">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="3">
        <location>
            <begin position="129"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="368" mass="41915" checksum="0E3F78F2CAEB38C1" modified="2011-11-16" version="1">MSFVPRTMFPKYNISLSNFKGHHQKALTKFGHLAPMIDLVVEVRDGRAPLATSNVLFDRVLSGKKKLVLYSKKDLSIINTKLLDKWHSSSNEDYMYVNCNNNTDVKKIINKIKQEYHDYDLPPPLGLRAMIIGMPNVGKSTLVNKLREVGNPVNKNAISQKQTKVAKVGGQPGVTRNTSEVIRICKNPDLFVYDTPGVFLPTVKDPETMLTLGLVGCVHESFIDPIILADYLLYLLNLQDPRGVLYADFMDHPTNDIYELLHYIAKARGVLQSDGAYDEVGIANFWVNMWKQGRNFDGRALFDIAAIKEVNNNEYMTLRKQEMERVSSTKVHEKIVEKLGQDGTAKSKHRKRSAKDREYDIRNRLFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIF8</accession>
    <name>G3AIF8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphomutase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_59859</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374012.1">
        <property type="nucleotide sequence ID" value="XM_007373950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIF8"/>
    <dbReference type="FunCoup" id="G3AIF8">
        <property type="interactions" value="174"/>
    </dbReference>
    <dbReference type="GeneID" id="18873107"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59859"/>
    <dbReference type="eggNOG" id="KOG4754">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039184_0_2_1"/>
    <dbReference type="InParanoid" id="G3AIF8"/>
    <dbReference type="OMA" id="PSESWHH"/>
    <dbReference type="OrthoDB" id="496981at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF1">
        <property type="entry name" value="HISTIDINE PHOSPHATASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="300" mass="34492" checksum="CC5D26CCA4EC67FB" modified="2011-11-16" version="1">MSLLVPDKRDHQDAHADLDQDKKYHFHLSQTTPKWLFEPVDGFFLQSAAETDDMEFRYTLQDFGAAQPWSHLIKRLDELNSQSESGVRYKLLFLARHGQGWHNVVTKKYSKADWFSKWRYLGTDGDLVWGPDAKLTQLGIDQARENHQAWKLQLSKGCPMPDKFYVSPLSRSINTHNITWPDTNPVVIDKLRETIGVHLCHKRSTKTEISEMFPNVEFEPGFAEEDVLFDKVFSSIREELHQQFLRIHGVLSDLFDDDDSVISITSHAGTIRSFITVIGHRKFTIPTGGMIPIVVKGVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIL0</accession>
    <name>G3AIL0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">asparaginase</fullName>
            <ecNumber evidence="2">3.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_59088</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-asparagine + H2O = L-aspartate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21016"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="EC" id="3.5.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the asparaginase 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.1" evidence="2"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373309.1">
        <property type="nucleotide sequence ID" value="XM_007373247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIL0"/>
    <dbReference type="FunCoup" id="G3AIL0">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIL0"/>
    <dbReference type="GeneID" id="18872766"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59088"/>
    <dbReference type="eggNOG" id="KOG0503">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019134_1_1_1"/>
    <dbReference type="InParanoid" id="G3AIL0"/>
    <dbReference type="OMA" id="RYYMQPL"/>
    <dbReference type="OrthoDB" id="542841at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006530">
        <property type="term" value="P:L-asparagine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08964">
        <property type="entry name" value="L-asparaginase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1170:FF:000001">
        <property type="entry name" value="L-asparaginase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.40:FF:000006">
        <property type="entry name" value="L-asparaginase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004550">
        <property type="entry name" value="AsnASE_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020827">
        <property type="entry name" value="Asparaginase/glutaminase_AS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027475">
        <property type="entry name" value="Asparaginase/glutaminase_AS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00520">
        <property type="entry name" value="asnASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707:SF28">
        <property type="entry name" value="60 KDA LYSOPHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500176">
        <property type="entry name" value="L_ASNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00144">
        <property type="entry name" value="ASN_GLN_ASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00917">
        <property type="entry name" value="ASN_GLN_ASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="L-asparaginase N-terminal" evidence="10">
        <location>
            <begin position="75"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase C-terminal" evidence="11">
        <location>
            <begin position="286"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="active site" description="O-isoaspartyl threonine intermediate" evidence="5">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="160"/>
            <end position="161"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001220-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001220-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW33725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="407" mass="44571" checksum="30E048BDE3F205B9" modified="2011-11-16" version="1">MSDSKKNVLFGPEYAESIPSITSFVPLSTISETAVEIENDTRDFDHPTFKLRYRSNSVVSNQSSEQSVYDTLPVIKVLGTGGTIASKGVTSHQTAGYQVDLTIEELIENIPDLSTTCQLEYEQVLNIDSKEMGTDELLLLYSKIQEDLPKFDGIVITHGTDTMEETAFFIQSTVNTFKPIVFCGSMRPSTAISSDGPMNLYQAIVIASSRESRGRGVLIALNDRIGSGFYITKSNANSLDTFKSIGQGYVGNFVNNEIHYYYPPSKPLGLTYFHLDATKTKSLPEVPIIYAHQGLNNKLIQLAISELGAEGLVLATMGAGSMSNISNQFLADLGLDEEFPVIYSKRSMDGMVPLGSLPQVTSKDSMVLFANAVAGGYLNPQKARILLQLCLNEGYGLREIKQVFSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIL7</accession>
    <name>G3AIL7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NADH-ubiquinone oxidoreductase 78 kDa subunit, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_48852</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the complex I 75 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373316.1">
        <property type="nucleotide sequence ID" value="XM_007373254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIL7"/>
    <dbReference type="STRING" id="619300.G3AIL7"/>
    <dbReference type="GeneID" id="18871275"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48852"/>
    <dbReference type="eggNOG" id="KOG2282">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_11_6_1"/>
    <dbReference type="InParanoid" id="G3AIL7"/>
    <dbReference type="OMA" id="QAMAYGV"/>
    <dbReference type="OrthoDB" id="10249365at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02773">
        <property type="entry name" value="MopB_Res-Cmplx1_Nad11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.740:FF:000016">
        <property type="entry name" value="NADH dehydrogenase (Quinone), G subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.740:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.210:FF:000002">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 75 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000002">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 75 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000283">
        <property type="entry name" value="NADH_UbQ_OxRdtase_75kDa_su_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054351">
        <property type="entry name" value="NADH_UbQ_OxRdtase_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010228">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019574">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015405">
        <property type="entry name" value="NDUFS1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050123">
        <property type="entry name" value="Prok_molybdopt-oxidoreductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01973">
        <property type="entry name" value="NuoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105:SF13">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 75 KDA SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105">
        <property type="entry name" value="RESPIRATORY NITRATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13510">
        <property type="entry name" value="Fer2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22151">
        <property type="entry name" value="Fer4_NDSU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22117">
        <property type="entry name" value="Fer4_Nqo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10588">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09326">
        <property type="entry name" value="NADH_dhqG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00929">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51839">
        <property type="entry name" value="4FE4S_HC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00641">
        <property type="entry name" value="COMPLEX1_75K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00642">
        <property type="entry name" value="COMPLEX1_75K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00643">
        <property type="entry name" value="COMPLEX1_75K_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="12">
        <location>
            <begin position="24"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S His(Cys)3-ligated-type" evidence="14">
        <location>
            <begin position="102"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="13">
        <location>
            <begin position="241"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW33732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="720" mass="78837" checksum="ABE44B4C35023AFD" modified="2011-11-16" version="1">MHSIKSHILRSSKRYISASTKRLAEVEVTIDGRKVSIEAGSSIIQAADLAGVPIPRYCYHDKLAIAGNCRMCLVDVERMPKLIASCAMPVQQGMVVHTDSERVKKSREGVTEMLLENHPLDCPVCDQGGECDLQEQSQRYGSDRGRFRELVGKRAVENKAIGPLVKTSMNRCIHCTRCVRFMNDVAGAPEFGTAGRGNDMQIGTYIERNINTEMSGNIIDLCPVGALTSKPYAFRARPWELKRTETIDVMDAIGSNIRVDARGIEVMRVLPRLNDEVNEEWISDKTRFACDGLKTQRLTTPLIRNGDKFEASTWDEALSTIASAYSKINPQGGELKAISGALADAESLVVLKDLVNKLGSENVTTDVAQGVNAHGLDIRSNYIFNSTIDGIEEADQILLVGTNPRHEAAVLNTRIRKVWLRSNLEISSVGQEFDSTFNVENLGSDATALQKALDGQLGQKLAAAKKPLIVIGSGVADSEDSSAIYKLVGDFASKHANFNSGEWNGVNLLHREASRVAALDIGFNTLVEETAKPKFIYLLGADEITNKDIPKDAFVVYQGHHGDVGASYADVILPGSAYTEKSATYVNTEGRTQVTRAATNAPGAAREDWKIIRALSEYLNAKLPYDDIVAVRTRLGEIAPHLVRHEVIEPVSQDIVKIGFADLVNKNKLAKITGSPLKNPIDNFYFTDVISRSSPTMAKCVVSFGAQIQKITDEKPDINF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJA7</accession>
    <name>G3AJA7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_59994</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374150.1">
        <property type="nucleotide sequence ID" value="XM_007374088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJA7"/>
    <dbReference type="GeneID" id="18873170"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59994"/>
    <dbReference type="HOGENOM" id="CLU_3088792_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJA7"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="52" mass="6008" checksum="8E752A417224B267" modified="2011-11-16" version="1">MNEINLREFLSNEMLLYKQFGYPDKGSYCIDNKFNCCLGGCQLAGPSEIYIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJB4</accession>
    <name>G3AJB4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cullin family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_60000</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374157.1">
        <property type="nucleotide sequence ID" value="XM_007374095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJB4"/>
    <dbReference type="STRING" id="619300.G3AJB4"/>
    <dbReference type="GeneID" id="18873173"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60000"/>
    <dbReference type="eggNOG" id="KOG2166">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_515868_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJB4"/>
    <dbReference type="OMA" id="MAHFEDF"/>
    <dbReference type="OrthoDB" id="27073at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031461">
        <property type="term" value="C:cullin-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000014">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016157">
        <property type="entry name" value="Cullin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01256">
        <property type="entry name" value="CULLIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Cullin family profile" evidence="5">
        <location>
            <begin position="74"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="487" mass="56375" checksum="EE137A3F1DB953D3" modified="2011-11-16" version="1">MVETTSGEKKKPSSSNTVLFASKWIETILKYRVQLVEVCKVSFDLNPIVEQTITMAMRDFINDKKKRSNIDAVNAPEMLSVYIDYFIKQLNKGSTTKDNSLKSGDQVEDFTNKSIQFLKFIKDKDAFEANYANHFAKRFLNSKTSLTNMIKGIDIEELVLGKLCDELGTSSLNKIIKMNKDIKSSKDLTHEWRNYVSNQKQSLIDLDLKICNVTDWPKSMTKDYKWFSKQENEATPFIWPRQLRNTMREFEEYWTTNKNNNNKSLYWSPKFGSMDMRITYPSRTYEINMSTYAGIIMLLFAPQSTNPDGSFVSAFEEKKELTYDEIAELTGIPELELKRHLQSIAVNPKSRLLIKVPMSKDVNNTDVFKLNEKFKSPSVKVKVLTVSSSSSASTAKVPVRTEQQEESEEVENNILEGRKLQVNAAIVRIMKSRHSMKHHELVEELIKQLASRFQPSMILIKQRIEDLIDKEYLTRDDSDRNVYHYVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJI1</accession>
    <name>G3AJI1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_148071</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373468.1">
        <property type="nucleotide sequence ID" value="XM_007373406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJI1"/>
    <dbReference type="GeneID" id="18870775"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_148071"/>
    <dbReference type="eggNOG" id="ENOG502RQD5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_178668_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJI1"/>
    <dbReference type="OMA" id="VKFWQPL"/>
    <dbReference type="OrthoDB" id="4080273at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="81" mass="9270" checksum="72D5304E062B7F17" modified="2011-11-16" version="1">MSSNGAKVLAIVFAGAVSWYTGVKFWKPIVVESLEKRGMLRDDIDIGYVDRDQPESWKDVVDKYKEFVHPNAQDPNEPKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJY0</accession>
    <name>G3AJY0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sm protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_134699</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the snRNP SmB/SmN family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373615.1">
        <property type="nucleotide sequence ID" value="XM_007373553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJY0"/>
    <dbReference type="FunCoup" id="G3AJY0">
        <property type="interactions" value="379"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJY0"/>
    <dbReference type="GeneID" id="18869844"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134699"/>
    <dbReference type="eggNOG" id="KOG3168">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_4_2_1"/>
    <dbReference type="InParanoid" id="G3AJY0"/>
    <dbReference type="OMA" id="HDERIMI"/>
    <dbReference type="OrthoDB" id="2020720at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070990">
        <property type="term" value="F:snRNP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01717">
        <property type="entry name" value="Sm_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050914">
        <property type="entry name" value="snRNP_SmB/NAA38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED PROTEIN B AND N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="9"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW34031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="139" mass="15078" checksum="1FD407B7EA9E1523" modified="2011-11-16" version="1">MSSQNIAITKKTKMSDLINYRIRIITIDNRTYIGTLLAFDSHLNLVISDTIESRITKKSWANLKKNNSAGATSGTSKDVVLDKRNLGLIILRGDQIISLSIESSPVVPVSSRVSGAKGKGTTRPLKTPVSVKGKLKKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK64</accession>
    <name>G3AK64_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptide hydrolase</fullName>
            <ecNumber evidence="9">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_60220</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M28 family. M28A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="9"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374390.1">
        <property type="nucleotide sequence ID" value="XM_007374328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK64"/>
    <dbReference type="FunCoup" id="G3AK64">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK64"/>
    <dbReference type="MEROPS" id="M28.001"/>
    <dbReference type="GeneID" id="18873280"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60220"/>
    <dbReference type="eggNOG" id="KOG2195">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024336_0_1_1"/>
    <dbReference type="InParanoid" id="G3AK64"/>
    <dbReference type="OMA" id="PNYEYEV"/>
    <dbReference type="OrthoDB" id="10013407at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03876">
        <property type="entry name" value="M28_SGAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000093">
        <property type="entry name" value="Peptide hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041756">
        <property type="entry name" value="M28_SGAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF17">
        <property type="entry name" value="AMINOPEPTIDASE Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <keyword evidence="8 9" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005131600" description="Peptide hydrolase" evidence="9">
        <location>
            <begin position="21"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="11">
        <location>
            <begin position="198"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="12">
        <location>
            <begin position="310"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW32875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="560" mass="61438" checksum="C15D83405663B1A3" modified="2011-11-16" version="1">MKLHSLSLATCLTLISSINALPSPWGAILGGSSSSQDDVVLSLDSKFPNLFSHEKDGNKHPKSKHHGNHHDHHDILSSIIDETDYNSLPEIDTEELQALITKHALEHRAKKLFHIAKSSVDEYGHPTRVIGSPGHLNTIKYIAKELKKLGGYYTVKTQKFDAIDGKVKSFSLLIDGQKPKSLEALSLSPPTPDGRPVHGNLVLVDNFGCSLGDFPEFAKDNIVLIKRGECAFGDKSVNAGISGAKGAIIYDSNGPLHGTLGTPTGKEVATVSVSEQDVQDYINALLKDPQHQFETTLYVDSYIKNISTLNVIADTVFGDHDNVVALGAHSDSVGEGPGINDDGSGTISLLEVAKQLTKFKVNNAVRFAWWAAEEEGLLGSTYYADNLEAKENSKVRLFMDYDMMASPNYEYEVYDANNQDHPNGSGNLKQLYIDWYVSHGLNYTLIPFDGRSDYVGFIDNGIPAGGIATGAEGVKDEKSKEKFGGKTGEWFDPCYHQLCDNLDNPDYQAWEINTKLIAHSVAVYAKSFEGFPEREVKEVSADSKKNNSNNFMYRGPHLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK97</accession>
    <name>G3AK97_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cell wall mannoprotein PIR1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_151845</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PIR protein family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375071.1">
        <property type="nucleotide sequence ID" value="XM_007375009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK97"/>
    <dbReference type="GeneID" id="18870910"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_151845"/>
    <dbReference type="eggNOG" id="ENOG502QQD8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2291342_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK97"/>
    <dbReference type="OrthoDB" id="5415592at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054508">
        <property type="entry name" value="PIR1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051153">
        <property type="entry name" value="Yeast_CWMannoprotein_PIR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254:SF1">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22799">
        <property type="entry name" value="PIR1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Cell wall mannoprotein PIR1-like C-terminal" evidence="6">
        <location>
            <begin position="15"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF22799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="104" mass="11465" checksum="1E5C1629FBB87976" modified="2011-11-16" version="1" fragment="single">CITKNALLLNLHDSILTDANGRIGSIVANHQFQFDGPPPQPDSLYTSGWSIINQDGNYLLALGKQTVFWECAAGGFFKLYDASIGAQCKQVELVVLKADYCQKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKA8</accession>
    <name>G3AKA8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">THIF-type NAD/FAD binding fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_60905</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the ATP-dependent dehydration of threonylcarbamoyladenosine at position 37 (t(6)A37) to form cyclic t(6)A37 (ct(6)A37) in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HesA/MoeB/ThiF family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375082.1">
        <property type="nucleotide sequence ID" value="XM_007375020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKA8"/>
    <dbReference type="FunCoup" id="G3AKA8">
        <property type="interactions" value="195"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKA8"/>
    <dbReference type="GeneID" id="18873630"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60905"/>
    <dbReference type="eggNOG" id="KOG2018">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013325_9_3_1"/>
    <dbReference type="InParanoid" id="G3AKA8"/>
    <dbReference type="OMA" id="GSWVVTM"/>
    <dbReference type="OrthoDB" id="10265862at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061503">
        <property type="term" value="F:tRNA threonylcarbamoyladenosine dehydratase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061504">
        <property type="term" value="P:cyclic threonylcarbamoyladenosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00755">
        <property type="entry name" value="YgdL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000125">
        <property type="entry name" value="tRNA threonylcarbamoyladenosine dehydratase 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43267">
        <property type="entry name" value="TRNA THREONYLCARBAMOYLADENOSINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43267:SF2">
        <property type="entry name" value="TRNA THREONYLCARBAMOYLADENOSINE DEHYDRATASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442415" description="THIF-type NAD/FAD binding fold domain-containing protein" evidence="14">
        <location>
            <begin position="19"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="15">
        <location>
            <begin position="57"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW33567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="403" mass="44823" checksum="7FBF42052F25F341" modified="2011-11-16" version="1" fragment="single">MINKSVLLSIIATAAVTASVLEFIHSYQTKSKPQLKAKEVAASPKQHSEELIREQLARNYAFLTEEGMTKVRSQRIIIVGLGGVGSWVATMLVRSGVSHIRVIDFDQVSLSSLNRHAVATLSDVGISKVDCLKNHLLQIAPWAEIDTCNQLWNMDSAEELIFGNDFNPTYVIDCIDNRDTKLDLLTYCYQKGIKVVSSGGAACKSDPTRINVADISKTEEDPLAKSIRVILKKRGIVDGIPTIFSAEKPDPRKAKLIPLSEDEITKGKVDELSALQDFRVRILPVLGTMPGMFGLAIATHVLTTVAGYPTEPVEGKNRYKVYNDILLSLAGQQSRIGKEDQRIQIALNDINYIVEEVFRGKSPISNFSTRLALSRWNKDEDISFQNVVVMTKEEQKEHEKRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKG1</accession>
    <name>G3AKG1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_60263</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374433.1">
        <property type="nucleotide sequence ID" value="XM_007374371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKG1"/>
    <dbReference type="GeneID" id="18873302"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60263"/>
    <dbReference type="eggNOG" id="ENOG502RQBH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158089_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKG1"/>
    <dbReference type="OMA" id="NAGRHYN"/>
    <dbReference type="OrthoDB" id="4090363at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="93" mass="10092" checksum="3E92761A63DC136F" modified="2011-11-16" version="1" fragment="single">MPDLFDNLFNKIGAKINGGKTTHYHGGASQVNTGRFYSYVATPNNNNYWLPREKQASATAAAMSADDTTINIKERSSSIVSMEESQSRSGSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL21</accession>
    <name>G3AL21_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Palmitoyl-protein thioesterase 1</fullName>
            <ecNumber evidence="1">3.1.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Palmitoyl-protein hydrolase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60380</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.2.22" evidence="1"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374579.1">
        <property type="nucleotide sequence ID" value="XM_007374517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL21"/>
    <dbReference type="STRING" id="619300.G3AL21"/>
    <dbReference type="GeneID" id="18873377"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60380"/>
    <dbReference type="eggNOG" id="KOG2541">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1315587_0_0_1"/>
    <dbReference type="InParanoid" id="G3AL21"/>
    <dbReference type="OMA" id="MEMPLCK"/>
    <dbReference type="OrthoDB" id="10263094at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002472">
        <property type="entry name" value="Palm_thioest"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247:SF8">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE/DOLICHYLDIPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02089">
        <property type="entry name" value="Palm_thioest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00414">
        <property type="entry name" value="PPTHIESTRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="244" mass="27675" checksum="97A2449EE0FE2A15" modified="2011-11-16" version="1" fragment="single">MLLSGLFNIQYLISSLAESFVDSGSKINLHQNFHVNDHMQLPLLNTPDKTEALPVVIWHGLGDNYNSSGINEVKEIFDTIYPGIFVYSISLADDPSADQQRSLFGDANIDVDQICQTLANITELSHGFNAIGFSQGGVLLRGLIERCSNITVHNLITFGSPHLGVSELPMCKPRDWLCKARNNVLKRQVWLDKVQKNVIPAQYFRDPLQYEQYLKYSHFLADINNERDEKNATYKENFSKINKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL42</accession>
    <name>G3AL42_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endoribonuclease YSH1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Endoribonuclease ysh1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12 14">mRNA 3'-end-processing protein YSH1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_66091</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the cleavage factor I (CF I) involved in pre-mRNA 3'-end processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF2/YSH1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374600.1">
        <property type="nucleotide sequence ID" value="XM_007374538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL42"/>
    <dbReference type="FunCoup" id="G3AL42">
        <property type="interactions" value="1003"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL42"/>
    <dbReference type="GeneID" id="18875082"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66091"/>
    <dbReference type="eggNOG" id="KOG1137">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009673_2_3_1"/>
    <dbReference type="InParanoid" id="G3AL42"/>
    <dbReference type="OMA" id="CKQHITL"/>
    <dbReference type="OrthoDB" id="10249535at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031126">
        <property type="term" value="P:sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034247">
        <property type="term" value="P:snoRNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16292">
        <property type="entry name" value="CPSF3-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000001">
        <property type="entry name" value="Cleavage and polyadenylation specificity factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10890:FF:000004">
        <property type="entry name" value="Cleavage and polyadenylation specifity factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021718">
        <property type="entry name" value="CPSF73-100_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF11">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11718">
        <property type="entry name" value="CPSF73-100_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01098">
        <property type="entry name" value="CPSF73-100_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="16">
        <location>
            <begin position="24"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="17">
        <location>
            <begin position="255"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA 3'-end-processing endonuclease polyadenylation factor C-term" evidence="18">
        <location>
            <begin position="536"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="489"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00075008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW33085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="762" mass="85375" checksum="6A0D6C24352CF4EB" modified="2011-11-16" version="1">MTVEEFSNEESFKFFGLGGCNEVGRSCHIIEYKNKVIMLDAGIHPALTGHSSFPFYDEYDLSKVDILLISHFHLDHAASLPYVMQQTTFKGRVFMTQATKAIYRWLLQDFVRVTSIGTTKMEGGEGQSSNLYTADDIMKSFDRIETIDYHSTMEIEGIKFTAYHAGHVLGACMYFIEIGGLKVLFTGDYSREENRHLHAAEIPPVKPDILISESTFGTGTLESKADLEKKLTNHIHATLTKGGRVLLPVFALGNTQELLLILDEYWNNNEDLQNINVYYASSLAKKCMAVYETYTSIMNDKIRLSASSSGHKSNPFDFKYIKSIRDLGKFQDMGPSVVIAAPGMLQAGISRQLLEKWAPDPKNLVILTGYSVEGTMAKELLKEPHTIQSVNNPEMTIPRRIGIEEISFAAHVDFQQNSEFIEKVSPSKIILVHGDSVPMGRLKSALLSKYSSRKGTEQEVKVFNPKNCEEVKIGFKGLKVAKVLGSLAEEQLSMLKKEIETKIETENEKIVEIKEEMEVDANDGDKNEAGVDTLHTGQFLSGVLVSKDFDLNLLQLQDLHEFTQLSTSFVKSKMNLRIHADISLMIYHLEQMFGYINILNDDEEEWECVIMDVVDIFVDKTKTGPGLQISVEWINDNLMADSLADSVIAILYSIDSSPASVKLSSTTHTHVKQETESKSAHAKSEIADRIKRISTLLRAQFGDCLQDLDNDKATITIGKHVAKIDYLNLTVECPSKVIKDRVESVIRRGCTLTAPLSQFQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL84</accession>
    <name>G3AL84_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_60439</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374642.1">
        <property type="nucleotide sequence ID" value="XM_007374580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL84"/>
    <dbReference type="FunCoup" id="G3AL84">
        <property type="interactions" value="284"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL84"/>
    <dbReference type="GeneID" id="18873409"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60439"/>
    <dbReference type="eggNOG" id="KOG0605">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_67_4_1"/>
    <dbReference type="InParanoid" id="G3AL84"/>
    <dbReference type="OMA" id="TVCNDRI"/>
    <dbReference type="OrthoDB" id="18472at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035974">
        <property type="term" value="C:meiotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120105">
        <property type="term" value="C:mitotic actomyosin contractile ring, intermediate layer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034973">
        <property type="term" value="C:Sid2-Mob1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044878">
        <property type="term" value="P:mitotic cytokinesis checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903501">
        <property type="term" value="P:positive regulation of mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903473">
        <property type="term" value="P:positive regulation of mitotic actomyosin contractile ring contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902846">
        <property type="term" value="P:positive regulation of mitotic spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902854">
        <property type="term" value="P:positive regulation of nuclear migration during mitotic telophase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905758">
        <property type="term" value="P:positive regulation of primary cell septum biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031028">
        <property type="term" value="P:septation initiation signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05600">
        <property type="entry name" value="STKc_Sid2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000141">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000319">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000109">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF417">
        <property type="entry name" value="CELL CYCLE PROTEIN KINASE DBF2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="59"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="13">
        <location>
            <begin position="360"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW33127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="471" mass="55138" checksum="7F85D4DA54A2651F" modified="2011-11-16" version="1">MFDYVISRRERTHLVEQKLLTDANYSNSPQKQQLEWKNYIGRERALLRKRRLKPKHKDFEMITQVGQGGYGQVFLARKKDTREICALKILNKKLLVKLDETRHVLTERDILTNTRSEWLVKLLYAFQDPEKVFLAMEFVPGGDFRTLLNNTGYLIPPHARFYISEMFAAVNSLHELGFTHRDLKPENFLIDSKGHIKLTDFGLAAGTVSNDRIESMKLKLQNLQDLETYQLPSRLMLERQKLFKQSQGHHNLANSIVGSPDYMALEVLEGKSYDYTIDYWSLGCMLFEALCGYPPFSGSKQDETYYNLKHWKTALRRPQTKDGRFVFSDRTWSLITKLIAAPNQRIRNFKQIQQMSYFVDIKDWDKLRERTPPFTPQLDNEEDAGYFDDFEDEEMMMKYKDVFARQEQNEQLLEKSGDNKKFQQNNFIGFTFKHKLNPNNKFGNGEINLLNMGAGAPGNNSRRLDPLATLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALA3</accession>
    <name>G3ALA3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Agglutinin-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_55077</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ALS family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374661.1">
        <property type="nucleotide sequence ID" value="XM_007374599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALA3"/>
    <dbReference type="FunCoup" id="G3ALA3">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALA3"/>
    <dbReference type="GeneID" id="18871908"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55077"/>
    <dbReference type="eggNOG" id="ENOG502QPQC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031316_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALA3"/>
    <dbReference type="OMA" id="CCAKSIA"/>
    <dbReference type="OrthoDB" id="3981162at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903561">
        <property type="term" value="C:extracellular vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030446">
        <property type="term" value="C:hyphal cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030445">
        <property type="term" value="C:yeast-form cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044403">
        <property type="term" value="P:biological process involved in symbiotic interaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030448">
        <property type="term" value="P:hyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2430">
        <property type="entry name" value="Agglutinin-like protein, N-terminal domain, N2 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008966">
        <property type="entry name" value="Adhesion_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008440">
        <property type="entry name" value="Agglutinin-like_ALS_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024672">
        <property type="entry name" value="Agglutinin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043063">
        <property type="entry name" value="Agglutinin-like_N_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033504">
        <property type="entry name" value="ALS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011252">
        <property type="entry name" value="Fibrogen-bd_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793:SF2">
        <property type="entry name" value="AGGLUTININ-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793">
        <property type="entry name" value="ALPHA-AGGLUTININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05792">
        <property type="entry name" value="Candida_ALS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11766">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01056">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49401">
        <property type="entry name" value="Bacterial adhesins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0134">Cell wall</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0843">Virulence</keyword>
    <feature type="domain" description="Agglutinin-like protein N-terminal" evidence="16">
        <location>
            <begin position="3"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW33146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="347" mass="38422" checksum="D075CB068D993D23" modified="2011-11-16" version="1">MSAGDTFTLTMPCVFKFTSDAKHVDLNATHSITGATTTFATCYFNPGELLVTYSELLCVLSDEVDSETKAYGAITIPVTFNNGRGASDTDLECAAMFHSGTTTVSFRDGDNILSIDVEFEKGCEYLNPLLRHNRISLLFDNSQLVIFAAECPDGYDSGTLGIQLVYEEHTINCNYARAAITNELNDWCCAKSIAPFTFTTICNQSTFIVEYENIPAGYRPFLDILVRAPPPEKMNMTYIETHYCSGSSELIDNTYLEYWGSYINKNPWSDGREIVLTTRTYTGKYTTVTTLSFDTSLDKTKTIVVEVPIPTTTITTTYGGKVTTRTTKTVEPGETATVIELPDYVTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALD5</accession>
    <name>G3ALD5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rxt3-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60484</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374693.1">
        <property type="nucleotide sequence ID" value="XM_007374631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALD5"/>
    <dbReference type="STRING" id="619300.G3ALD5"/>
    <dbReference type="GeneID" id="18873438"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60484"/>
    <dbReference type="eggNOG" id="ENOG502QX51">
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="entry name" value="LCCL-like domain"/>
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        <property type="entry name" value="LCCL_sf"/>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="465" mass="52934" checksum="EB2A1A6420EB2F47" modified="2011-11-16" version="1">MSSDKQPPQLPHQGIRLPPISFLSGGGPSKLPPTSNLGIPNFLNNPSSPPSSSILLPTLTPPQSSAQPDEQANKRMKLYDSQSPPGSYATSPPQQKYSPQQLPHINQLQPSVQSNVQPSSYSPPVTQPEAKLEPEEKHDQEHAHAHAPQQQHHHHHHNHGHVHPHHHHHHHHHHHHHVDKEETGNPQVVSKVIKKSPPSFSIEPIVQTLKELFPKRHFLGTLIYNPTTTWETLQTSSLYGLKPEHHQRFNEIKLSYASRVKSEKVKYIPTIPPLPVEYINSVIEVKIPFRFIVQLKHDFINEIYVRDVWGGASGIYTDDSDILQVLVHMGLFNQSIDLSKWNPNWKSDDFIKPLTTTDNEGVDKDIYGDLSVQVLLLPGLSKYVGYTANKINSRSWKGQHSGLSMVVYNVKWDTRNGYLQDKSVYKKYENEISQDKADSELTRKEAVGWKFDYGLYKKINEKYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALJ2</accession>
    <name>G3ALJ2_SPAPN</name>
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            <fullName evidence="2">Membrane protein</fullName>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
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    </feature>
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        <source>
            <dbReference type="EMBL" id="EGW33235.1"/>
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        <source>
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    </evidence>
    <sequence length="195" mass="21583" checksum="39FAA0C8B0F632A9" modified="2011-11-16" version="1">MANLFDIEQHLVFYRSYHYNNVNVAIHLVCIPIILLSAITFLVPQNILGSAHPYINLGSALAWGYGIFYTLLDLKLGLPSFGLLVTYAHLIKGHYLSLSSTTPVTPGDFVKYAIAAHVAAWLAQFYGHGVHEKRAPALLDNLLQALVLAPFFVVFEIAFFLGFRSDLKKSMDAKASVNIAKFKKESAAAKEKKSK</sequence>
</entry>
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        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
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                <person name="Dale B.E."/>
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                <person name="Zinkel R."/>
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            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Amide_Lactam_Regulators"/>
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        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47171">
        <property type="entry name" value="FARA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47171:SF3">
        <property type="entry name" value="FARA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="56"/>
            <end position="87"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
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        <location>
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            <end position="775"/>
        </location>
    </feature>
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        <location>
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        <location>
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            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="909"/>
            <end position="921"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="975" mass="109634" checksum="7BB5E554BB736797" modified="2011-11-16" version="1">MKQDDEQTEIKQPSSVSSPSNGDSAKTNGGGSNSGNGEAKPKAFTIRYKRPRGSRACTVCRSRKVRCDAEIHIPCTNCITFGCDCILPEAKKRGNAAGATTTKSVKNKKKKDDNVKTQQNKKKKQKHSEDEDDSENQEGDDDHDYDEEHKAKKIKHDSHTSINHSANSSGTNGVTSSTNQAILNISQISVPPSLHSDYKNRPSIHKKEILSNKHPQKPALTFLGSSSNVVVPHAIGENHVRLTEDIFEVNENSLDSVELEILKLRGAFLLPPRELALDLIEAYFEHVHPLMPVINRTMFMKKFNDPNDSPSLMLLHAVLLCGCRVSKNPLLLDSSGSNSLAALTFFRRAKALYETNYESDPVSIIQTIILIGSYWDGPEDVTKNSFYWTRVAVGLAQGFGFQRDVSNSRNLTCSEKKLWRRIWWCLFEKDRNVAIAFGRPIVIDLNDCDVPMLTLDDFNEDDPELGIVSPYPVNETQALYFIHLVKLAEITGIIIKHQYSVKSESMKLKNVFSIIEHCDMLMGIWFTNLPPQLVFSLTDNATQNFYSCLLNAQYYNRLYLIHRSNLIRMAKSSSTNPNNYKYPSWGISFQSARMISIISKILLDRDQIKYVPSMYVYITFSALVMLIYHVDSANTVIASTASDSLYVSRSVLKELSKYWPIAGVLMKLFDKYANDKWKRAKVIEHRSQHAEYTENKKGGYMGLYSQVPVQPSQQQVQQQAQELSPHNDGSSIYSGASGISPGGNVYDYPAFSSQAQLNNQSNSVRQVATPTVTTPQQQQQQQQQQQSNDIQTQAAAPNIEDLIQQYKIPMKRAAESEQSTSTSDTSPTSSTKSFPEINLVTDSQIATQQNFFENFEPTQLFPNFSQPPTRSQSPHPDHQVGSNVYQSLEHLSGIEERRDSEDRENEPKQQSQPQSQVVQPSPHVAMIPDENYTPGFNPNTPGFQTNFVDSSYINMNLQSGVEMQDDFTGLFNFVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALW9</accession>
    <name>G3ALW9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 14">Sulfiredoxin</fullName>
            <ecNumber evidence="4 14">1.8.98.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_55210</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>S-hydroxy-S-oxy-L-cysteinyl-[peroxiredoxin] + [protein]-dithiol + ATP = S-hydroxy-L-cysteinyl-[peroxiredoxin] + [protein]-disulfide + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17545"/>
            <dbReference type="Rhea" id="RHEA-COMP:10593"/>
            <dbReference type="Rhea" id="RHEA-COMP:10594"/>
            <dbReference type="Rhea" id="RHEA-COMP:13681"/>
            <dbReference type="Rhea" id="RHEA-COMP:17976"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:61973"/>
            <dbReference type="ChEBI" id="CHEBI:61974"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="1.8.98.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the sulfiredoxin family.</text>
    </comment>
    <dbReference type="EC" id="1.8.98.2" evidence="4 14"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374837.1">
        <property type="nucleotide sequence ID" value="XM_007374775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALW9"/>
    <dbReference type="FunCoup" id="G3ALW9">
        <property type="interactions" value="349"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALW9"/>
    <dbReference type="GeneID" id="18871918"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55210"/>
    <dbReference type="eggNOG" id="KOG3388">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124532_1_1_1"/>
    <dbReference type="InParanoid" id="G3ALW9"/>
    <dbReference type="OMA" id="SQIRRPI"/>
    <dbReference type="OrthoDB" id="10023328at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032542">
        <property type="term" value="F:sulfiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032272">
        <property type="term" value="P:negative regulation of protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16395">
        <property type="entry name" value="Srx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1530.10:FF:000005">
        <property type="entry name" value="Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1530.10">
        <property type="entry name" value="Conserved hypothetical protein from pyrococcus furiosus pfu- 392566-001, ParB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016692">
        <property type="entry name" value="Sulfiredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21348">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21348:SF2">
        <property type="entry name" value="SULFIREDOXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017267">
        <property type="entry name" value="Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="ParB/Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="7 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB-like N-terminal" evidence="17">
        <location>
            <begin position="8"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="76"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Interchain" evidence="16">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR017267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR017267-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR017267-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW33322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="122" mass="13794" checksum="C4BCD2D90ACED3B0" modified="2011-11-16" version="1">MNRQLKIEYVPLDQIRRPIPPVLDYQKIDAMVSTLNGKPMASATCKVEDITSGELPPIDVFKVRESGKNYYFAFGGCHRFQAYDRIDGESKEPTLVKCGIMPATRKTLKVYLGASVDEMFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMA2</accession>
    <name>G3AMA2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nuclear mRNA export factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_137326</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the SAC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374915.1">
        <property type="nucleotide sequence ID" value="XM_007374853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMA2"/>
    <dbReference type="FunCoup" id="G3AMA2">
        <property type="interactions" value="149"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMA2"/>
    <dbReference type="GeneID" id="18870039"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137326"/>
    <dbReference type="eggNOG" id="KOG1860">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006094_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMA2"/>
    <dbReference type="OMA" id="FQGTCLD"/>
    <dbReference type="OrthoDB" id="264795at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.990:FF:000008">
        <property type="entry name" value="Nuclear mRNA export protein SAC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017173">
        <property type="entry name" value="Sac3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045107">
        <property type="entry name" value="SAC3/GANP/THP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005062">
        <property type="entry name" value="SAC3/GANP/THP3_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024293">
        <property type="entry name" value="SAC3_helical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436">
        <property type="entry name" value="80 KDA MCM3-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436:SF3">
        <property type="entry name" value="GERMINAL-CENTER ASSOCIATED NUCLEAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12209">
        <property type="entry name" value="SAC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03399">
        <property type="entry name" value="SAC3_GANP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037320">
        <property type="entry name" value="mRNA_export_factor_Sac3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC3/GANP/THP3 conserved" evidence="7">
        <location>
            <begin position="208"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="SAC3 helical" evidence="8">
        <location>
            <begin position="763"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="723"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1216"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1223"/>
            <end position="1241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR037320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1258" mass="143203" checksum="38D979FD246948AC" modified="2011-11-16" version="1">MNINGSANNQKIFDNNYNDPRRSGNRKKFPNINGNTKSNKINNDQKRNKNSKSRFEEVAKESTYSPDPLDRPTKVIPRQNPGKIQSYSQQEIASIGGLFSNPEQLGFQRYSNKSIPPRPVPKYLLPQARLLHTPPFVQNQWDKENQALMVKMETENLSKGDYQGLYESFQKLREVERKKMEELGLVDAENIAKDLNDAISFQGSCLDMCPVFERVRRQLENNVKDLEKDPITKKISPQKAVKAFSRPAAGQPPPLPSEVRPPHILVQTLDYLIDNVIDHLPEAHSFIWDRTRSIRQDFTYQNSFGPEAIDCNERIVRIHLLSLHIMAGSDVEYSQQQELEQFNKALQTLMEIYTDVRNNGGSSPNEAEFRAYHLLSHIRDPELEREIQNLPNEIYNDSQVQIALTLRNIISQNNIVERGVTNTVGGLNLFVKFFRMVYSDQVPFLMTCLLETHFNEIRFYVLKAMARSYHSKGKPYSTEGLCDILGFDNVEQVIKFVSYYDIDVIQESSGHFVVDLFNKEKLETKYKLHSFHAKPKPSPYYSHRLNSKIQGLNWKYFINRGKPNQNLHLATQPKIISQDTIKKVEVMQQPSVNTGLFQPFTNVGGNIAPTEIRFDKPPALAAPATAPAPAPAAPVPPRMAKPDIEMNDDEIGPKTVPDLFSNIKPKTNISSGLFGMNKPKDAPPTKPTFDFNTVRPSTAPEPKPVFIPPVNQGTKQSPIKIESSPVAPIPPRSQSNVVVKPPTPEIIPPKRLVDSPHFSQAARETMNDIVRNCVDAELVNVITQCMRAHAIQTERARVIEKLSDDLYEAFLSEVIYETAAEAKADGYYQRCLTKRVIHQLADKSSKLLVQYQERKRRAQELESVSFRQHLKHTSRDRSETDKRLQYSNVDITNRQREVQALWAPIDLDSFMNLIVKNIKYKLSSEVQLKFLLVVENWSTSYSKWLNSKLQLKPDSKNMVYENLINDDDITVKFTSLPSQEYLTKEFFSNTSFVLFECGLTKPNGSIEEKLQRDFRVLRKIISLIDKYSYYKVHVLVVFWDATQSGITTEKVSELLNLASFNACSNLSNLILCDMTSSDINHILSQAFFKISQDFDGTLTSRGNKRKILLDREKQQKQATTYEPPAKIQKIETKMLSSVKNLRKYDYLQNHINSTGHPRYNTTNNSFNSTSMVASVQNRSLLPALIRNRSSVNNSTFFNSTISTIGNNDTSILRGFGQGVIGESTPSGSPKRNSKASLSTSLSQLKELTAGIKRKYSHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMG6</accession>
    <name>G3AMG6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_60162</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374333.1">
        <property type="nucleotide sequence ID" value="XM_007374271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMG6"/>
    <dbReference type="FunCoup" id="G3AMG6">
        <property type="interactions" value="598"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMG6"/>
    <dbReference type="GeneID" id="18873251"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60162"/>
    <dbReference type="eggNOG" id="KOG0810">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042423_0_1_1"/>
    <dbReference type="InParanoid" id="G3AMG6"/>
    <dbReference type="OMA" id="VLHASHY"/>
    <dbReference type="OrthoDB" id="10255013at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044853">
        <property type="term" value="C:plasma membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070056">
        <property type="term" value="C:prospore membrane leading edge"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070057">
        <property type="term" value="C:prospore membrane spindle pole body attachment site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032120">
        <property type="term" value="P:ascospore-type prospore membrane formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15849">
        <property type="entry name" value="SNARE_Sso1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.70:FF:000008">
        <property type="entry name" value="Syntaxin family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF307">
        <property type="entry name" value="PROTEIN SSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00804">
        <property type="entry name" value="Syntaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="8">
        <location>
            <begin position="191"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="290" mass="33547" checksum="99D88EF826A19E63" modified="2011-11-16" version="1">MSNPYQQATPNSYELDSYAGNKYSSNEDDFVQFMNEIQDINSQLDNYSHIINLINNKQRNYLHDIDLNDTDEDTRASAQIDNLVNEAQSLQLELKSRIKNVQTQAVHSRDQTKLDQAETCRKRFLDLIQEYRMVEANNKEENKQQAERQYRIINPNATDAELKAVVEDGSEQYFQQALLQSNRRGEARSVLNDVQTRHRELIKLEKTMAELTQLFHDMEELVIEQDQPIQQIEEQVAAAQHDIEQGVGHTNKAVKSAKAARKKKLWCFFICVIIIIILAVILGAYFGTRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMN2</accession>
    <name>G3AMN2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 13">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="2 13">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_60825</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17 13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="13">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="2 13"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374991.1">
        <property type="nucleotide sequence ID" value="XM_007374929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMN2"/>
    <dbReference type="FunCoup" id="G3AMN2">
        <property type="interactions" value="661"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMN2"/>
    <dbReference type="GeneID" id="18873590"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60825"/>
    <dbReference type="eggNOG" id="KOG0660">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="InParanoid" id="G3AMN2"/>
    <dbReference type="OMA" id="NRYTDLN"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001100">
        <property type="term" value="P:negative regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007231">
        <property type="term" value="P:osmosensory signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016241">
        <property type="term" value="P:regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033262">
        <property type="term" value="P:regulation of nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000049">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000050">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="23"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW33476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="383" mass="44097" checksum="E1EA8F8C75B538D3" modified="2011-11-16" version="1">MSSDGEFTRTQIFGTVFEITNRYTDLNPVGMGAFGLVCSAVDKLTGQNVAVKKIMKPFSTAVLAKRTYRELKLLKHLKHENLITLDDIFISPLEDIYFVNELQGTDLHRLLTSRPLEKQFIQYFTYQILRGLKYIHSAGVIHRDLKPSNILINENCDLKICDFGLARIQDPQMTGYVSTRYYRAPEIMLTWQKYDTEVDLWSVGCILSEMIEGKPLFPGKDHVHQFSIITELLGSPPQDVIDTIASENTLRFVRSLPHREPIPFGERFASCTHVEPEAIDLLAKMLVFDPKKRISAAQALTHPYMEPYHDPTDEPICETKFDWSFNDAELPVDTWRVMMYSEILDFHQNHGGIELQNQDSQQLSQDIVNVPQHQDQQVELKYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMV8</accession>
    <name>G3AMV8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_61450</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375648.1">
        <property type="nucleotide sequence ID" value="XM_007375586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMV8"/>
    <dbReference type="GeneID" id="18873900"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61450"/>
    <dbReference type="HOGENOM" id="CLU_2251736_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMV8"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="104" mass="12188" checksum="A22860C9404A1701" modified="2011-11-16" version="1">MAFSSSLSSVFPISEVQRFLSRQQRSLQRLLLGVKLDFGFVLDSFDEICKPVPGFENDHLMYESILGNLLKGDELRYPQLRHVVIDRYHYKIEGNSYKQINYEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN02</accession>
    <name>G3AN02_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">H/ACA ribonucleoprotein complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_61483</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ("psi") residues may serve to stabilize the conformation of rRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs (H/ACA snoRNPs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the GAR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375691.1">
        <property type="nucleotide sequence ID" value="XM_007375629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN02"/>
    <dbReference type="FunCoup" id="G3AN02">
        <property type="interactions" value="623"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN02"/>
    <dbReference type="GeneID" id="18873914"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61483"/>
    <dbReference type="eggNOG" id="KOG3262">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080002_1_0_1"/>
    <dbReference type="InParanoid" id="G3AN02"/>
    <dbReference type="OMA" id="KPQDGIV"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034513">
        <property type="term" value="F:box H/ACA snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000454">
        <property type="term" value="P:snoRNA guided rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.230:FF:000001">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.230">
        <property type="entry name" value="Probable tRNA pseudouridine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038664">
        <property type="entry name" value="Gar1/Naf1_Cbf5-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007504">
        <property type="entry name" value="H/ACA_rnp_Gar1/Naf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23237:SF6">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23237">
        <property type="entry name" value="NUCLEOLAR PROTEIN FAMILY A MEMBER 1 SNORNP PROTEIN GAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04410">
        <property type="entry name" value="Gar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 8" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="112"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="131"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="207" mass="21154" checksum="BB864B0B2E6464EE" modified="2011-11-16" version="1">MNRGRGGFRGGRGGGRPGGFIQQGPPDTVLEMGAFMKACEGDIVCRSINVKIPYFNAPIYLENKTQVGKVDEILGPLNEVFFTIKPSEGVKADSFKEGDKFYIGPDKLLPLERFLPKPPQVGPKPKRKSAGGRGGFGGDRGGRGGRGGFGGRGGSRGGFGGGRGGSRGGFGGRGGSRGGFGGDRGGRGGRGGFGGRGGSRGGRGGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN39</accession>
    <name>G3AN39_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Malate synthase</fullName>
            <ecNumber evidence="3 10">2.3.3.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">MLS1</name>
        <name evidence="14" type="ORF">SPAPADRAFT_139829</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>glyoxylate + acetyl-CoA + H2O = (S)-malate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the malate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.9" evidence="3 10"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375729.1">
        <property type="nucleotide sequence ID" value="XM_007375667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN39"/>
    <dbReference type="STRING" id="619300.G3AN39"/>
    <dbReference type="GeneID" id="18870241"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139829"/>
    <dbReference type="eggNOG" id="KOG1261">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018928_3_0_1"/>
    <dbReference type="InParanoid" id="G3AN39"/>
    <dbReference type="OMA" id="HLATIMV"/>
    <dbReference type="OrthoDB" id="186072at2759"/>
    <dbReference type="UniPathway" id="UPA00703">
        <property type="reaction ID" value="UER00720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004474">
        <property type="term" value="F:malate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00727">
        <property type="entry name" value="malate_synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1220.12:FF:000001">
        <property type="entry name" value="Malate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.360:FF:000001">
        <property type="entry name" value="Malate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.360">
        <property type="entry name" value="Malate synthase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1220.12">
        <property type="entry name" value="Malate synthase, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044856">
        <property type="entry name" value="Malate_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011076">
        <property type="entry name" value="Malate_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006252">
        <property type="entry name" value="Malate_synthA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019830">
        <property type="entry name" value="Malate_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001465">
        <property type="entry name" value="Malate_synthase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048355">
        <property type="entry name" value="MS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048356">
        <property type="entry name" value="MS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046363">
        <property type="entry name" value="MS_N_TIM-barrel_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01344">
        <property type="entry name" value="malate_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42902">
        <property type="entry name" value="MALATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42902:SF1">
        <property type="entry name" value="MALATE SYNTHASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20659">
        <property type="entry name" value="MS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20656">
        <property type="entry name" value="MS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01274">
        <property type="entry name" value="MS_TIM-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001363">
        <property type="entry name" value="Malate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51645">
        <property type="entry name" value="Malate synthase G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00510">
        <property type="entry name" value="MALATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4 10" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Malate synthase N-terminal" evidence="12">
        <location>
            <begin position="13"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Malate synthase TIM barrel" evidence="11">
        <location>
            <begin position="170"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Malate synthase C-terminal" evidence="13">
        <location>
            <begin position="423"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001363-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW32453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="551" mass="62514" checksum="15FDA65F7C9E0A38" modified="2011-11-16" version="1">MSSPFPKTADKLKGVQILGAVPSEARHILNPEALALVATLHRSFEATRQELLAARKNQQRLRDEGYLPDFLPETAYIRDDATWTGPPLAPCLEDRRVEITGPTDRKMVINALNSGVATYMADFEDSLAPTWHNLVDGQVNLYDAVRRNLTANINGKPYALNLDPGRRIPTLIVRPRGWHLTEKHVLVDGKPVSGGIFDFAIYFYNNAKESLNQGFGPYFYLPKMEHHLEAKLWNDIFNHAQNYVGIPRGTIRASVLIETLPAAFQMDEIIYQLRDHIAGLNCGRWDYIFSYIKSLRNHPEFILPDRSQVNMASPFMASYVKLLVHTTHKRKVHALGGMAAQIPIKEDPERNRIALENVAKDKLREVQRGCDSCWVAHPALVPVVLKVFNEHMKGPNQIDLPPREPYKKITQRDLLSPFVKDAKITEQGIRTNIVIGILYIEAWLRHVGCVPINFLMEDAATAEVSRSQIYQWVTHGAKTDAGKTVTKQYVKQLLEEEYAKLVKNARPGNKLKRAYEYFAPEALAERYSDFLTTLIYDEVTTIGRSVPAERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANA3</accession>
    <name>G3ANA3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">26S proteasome regulatory subunit RPN1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_138317</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the proteasome subunit S2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375762.1">
        <property type="nucleotide sequence ID" value="XM_007375700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANA3"/>
    <dbReference type="FunCoup" id="G3ANA3">
        <property type="interactions" value="1563"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANA3"/>
    <dbReference type="GeneID" id="18870112"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138317"/>
    <dbReference type="eggNOG" id="KOG2005">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008705_1_0_1"/>
    <dbReference type="InParanoid" id="G3ANA3"/>
    <dbReference type="OMA" id="GTCNGDI"/>
    <dbReference type="OrthoDB" id="10252509at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905754">
        <property type="term" value="C:ascospore-type prospore nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016643">
        <property type="entry name" value="26S_Psome_Rpn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002015">
        <property type="entry name" value="Proteasome/cyclosome_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041433">
        <property type="entry name" value="RPN1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040892">
        <property type="entry name" value="RPN1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01851">
        <property type="entry name" value="PC_rep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18051">
        <property type="entry name" value="RPN1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17781">
        <property type="entry name" value="RPN1_RPN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015965">
        <property type="entry name" value="26S_Psome_Rpn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RPN1 N-terminal" evidence="6">
        <location>
            <begin position="48"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="26S proteasome non-ATPase regulatory subunit RPN1 C-terminal" evidence="7">
        <location>
            <begin position="904"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="626"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="11"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="644"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="663"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="680"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR015965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="963" mass="106822" checksum="FDA339FA70347857" modified="2011-11-16" version="1">MAPAQQDTSSTQQQPAAVEEAATTTTTKKPVLPEEELSEEDQKLKDELELLVERLNEPNQQVELYGKYLDALKTFIKESTTSMTAVPKPLKFLRPHYPSLTDLYTQWSSETYSATEEIVVKLADILSVLAMTYSDDGKRESLKYRLLSQKESLPDWGHEYMRHLALEIGEAYQEYLGSADDEVEKLVKLSLQIVPFFLKHNAEADAVDLLLEIESIDKLPQFVDDNTFARVCLYMVSCVPLLAPPDDVSFLNTAFAIYLAHGQLTQALTLAIKLDDEELIKQVFNSTNDELIHKQLGFILSQQNNSFKYPGENPEVQETISNVKLAEYFKYLIKELNLLEPKVPEDIYKSHLENTKFGLGTSGSIDSAKQNLAAAFVNSFLNLGFGNDKLIQTSDDNKSWIYRTKGSGMSSTTASLGSIHQWDINEGLQVLDKYTYSDDPEIKAGALLGTGIVSSNVHDEVEAAFALLQEYVTDPNKLYQTSAINGLGIAFAGSANEEVLNLLLPLVSDLEISLEISCLAALALGHVFVGTCHGDITSTILQTLLERDFTQLTNKFIKFMALGLGLLYMGKTEQVEDVLETIDAIEHPISKTLKVLVNICAYAGTGNVLQIQALLQMCTAKPKEQAEEEKKLEQSEEDQQKEEEEAKKEHADTTGDVEMEDASEVKPETEAKAEVKDEDVTAEEDEDAEEQEELYQGIAVLGLACIAMGEDIGQDMSLRHFGHLMHYGNSLIKRAVPLAMGLVSVSNPQMKVFETLSRYSHDADLEVAQNAIYSMGLVGAGTNNARLAQLLRQLASYYIKSPESLFMVRIAQGILHLGKGTLTLTPFNSERTIISKVSLASLLTIAMALLDPREFILNDSSTETTHQLLYYLTPAIKPRMLITVDENLNPLKVNVRVGQAVDVVGQAGKPKTITGWVTQSTPVLLNYGERAELENTDEWISLSHSLDGIVILKKNPEYMEVDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANB6</accession>
    <name>G3ANB6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Membrane protein TMS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_61562</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375775.1">
        <property type="nucleotide sequence ID" value="XM_007375713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANB6"/>
    <dbReference type="FunCoup" id="G3ANB6">
        <property type="interactions" value="526"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANB6"/>
    <dbReference type="GeneID" id="18873956"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61562"/>
    <dbReference type="eggNOG" id="KOG2592">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029574_2_0_1"/>
    <dbReference type="InParanoid" id="G3ANB6"/>
    <dbReference type="OMA" id="DKHCNPL"/>
    <dbReference type="OrthoDB" id="5963193at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005016">
        <property type="entry name" value="TDE1/TMS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383">
        <property type="entry name" value="SERINE INCORPORATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383:SF9">
        <property type="entry name" value="SERINE INCORPORATOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03348">
        <property type="entry name" value="Serinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="368"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="406"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="438" mass="48775" checksum="B77C64E251313EBB" modified="2011-11-16" version="1">MTRITYAFILLVNSLLSWIALSPFIIHKLEKATFGYINNRCGPDGNQCISFTSVYRINFALGVLHLVLAALLLNVQSTANPRAMIQNGCWKMKIFAWICLIFVNFVLIPDNFFVFYGNNIAIIFSTIFLGIGLILLVDFAHAWAEKCLEKIELEELTGEGDAGFWKKLLIGGTLTMYISSIVLTVIMYWFFAGSGCSMNKTAITLNLIFSIIISAMSINTTIQEYNPHAGLAQSSMVVFYCTYLVMSAVASEPDDKYCNPLVRSKGTRTASVVLGAMFTFIAIAYTTTRAAANSAFSSDSNQEFLVGASTTTTQPAARNEMRYQAIKQAVDEGSLPESALTQMDLYNDDEEGATPDEERQSVQYNYSLFHIIFFLATQYVATLLTINVKQDDLGDFVPVGRTYFASWVKIVSAWVCFVLYGWSLVAPVIWPDRFGIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANI5</accession>
    <name>G3ANI5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Pterin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_61089</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(7,8-dihydropterin-6-yl)methyl diphosphate + 4-aminobenzoate = 7,8-dihydropteroate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19949"/>
            <dbReference type="ChEBI" id="CHEBI:17836"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="EC" id="2.5.1.15"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the C-terminal section; belongs to the DHPS family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375251.1">
        <property type="nucleotide sequence ID" value="XM_007375189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANI5"/>
    <dbReference type="FunCoup" id="G3ANI5">
        <property type="interactions" value="245"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANI5"/>
    <dbReference type="GeneID" id="18873705"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61089"/>
    <dbReference type="eggNOG" id="KOG2544">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008023_2_0_1"/>
    <dbReference type="InParanoid" id="G3ANI5"/>
    <dbReference type="OMA" id="FMYETEP"/>
    <dbReference type="OrthoDB" id="294251at2759"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00155"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004150">
        <property type="term" value="F:dihydroneopterin aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004156">
        <property type="term" value="F:dihydropteroate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00739">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00483">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.20:FF:000014">
        <property type="entry name" value="Folic acid synthesis protein fol1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.560">
        <property type="entry name" value="7,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="entry name" value="Dihydropteroate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045031">
        <property type="entry name" value="DHP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006390">
        <property type="entry name" value="DHP_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000550">
        <property type="entry name" value="Hppk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035907">
        <property type="entry name" value="Hppk_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01496">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01498">
        <property type="entry name" value="folK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941">
        <property type="entry name" value="FOLATE SYNTHESIS PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941:SF1">
        <property type="entry name" value="FOLIC ACID SYNTHESIS PROTEIN FOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01288">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55083">
        <property type="entry name" value="6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="Dihydropteroate synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00792">
        <property type="entry name" value="DHPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00793">
        <property type="entry name" value="DHPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00794">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pterin-binding" evidence="15">
        <location>
            <begin position="276"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW31975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="597" mass="66986" checksum="0C4DAC2019A1C984" modified="2011-11-16" version="1" fragment="single">TVEALVSNIGQLIFQNYDAGIESVFVKVTKPNAFSHIEGVGVSSYMNQKTFKDAQPLVLTQAPSSAFNMPVEEVTASTYEGDHIAFIAFGSNTGNQVENIQRALTLLQQYNIQVESTSSLYISKPMYYLDQPDFYNGVVKVSFKDLAPHKLLAILKEIEYKHINRVKEFDNGPREIDLDIVLYDDISLNTPDLIVPHKSMLERTFVLQPLCELLPPDQIHPISAEPFHNHLQQLFANAPAESLQESSQLLQFIPIPRLEARNNPLKFDQVYNKHPTLIMGILNMTPDSFSDGGKNFDKSLAEVVRSAEKLVEDGADIIDIGGVSTRPGSIEPSEEEEVKRVLPIVKAIRSSQNKKLANVLISIDTYRSKVAEECLIAGADIINDISMGKYDEKIFDVVAKYGCPYIMNHTRGTPETMSKLTKYESNTNDDIIEYVIDPTQGHQELSLPPNIKNLLNGVSRELAHQMFKAFERGVRKWQIIVDPGVGFAKNVQQNLALVKHASFFKKYSIQINERDDLVVKHKYLSFNGMSVLVGTSRKRFLGTITKQPTPTERVFSTGATVTSCIEQNTDIIRVHDVKAMKDVVITSDAVYRDIFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANM2</accession>
    <name>G3ANM2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_51095</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375827.1">
        <property type="nucleotide sequence ID" value="XM_007375765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANM2"/>
    <dbReference type="STRING" id="619300.G3ANM2"/>
    <dbReference type="GeneID" id="18871515"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51095"/>
    <dbReference type="eggNOG" id="ENOG502QWTH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017969_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANM2"/>
    <dbReference type="OMA" id="PQFRRNS"/>
    <dbReference type="OrthoDB" id="6287070at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF722">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="119"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="251"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="790"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="268"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="279"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="802"/>
            <end position="813"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="826" mass="93930" checksum="B6F4E2A570C00315" modified="2011-11-16" version="1">MLRRFKSNDPSRIFPDSFWLWYMDSYAKVDRETTIKHTPFSPIRYNDKSHSVTTIIKGFKHLSLPVPSVNAIIALLRSPFTRGDVIKTFHLLRVFQLSEQGLFLTNSGVDKFGCTIRYMGAENWDNVMCYMDALLFSMFANLESFEPILFISNSHNPDIDKLRALLRAYVTLLRSGNLITTDITVRLCELLAKLGFKEAMSHKQQDAAALFEFLTECLSMPLLTFKIDIKHGGKYSQTDDEKFSRERILFVSIPEEEEEEEESTKPGSSSVSQNSQISKPLSENSESSKPVSETPESKSESKLDSTPLSMDGPDKKSKDKSDEKSGTKEPESSNSSSSGCDEILLEECLEHYFNNTINVERELERELERRASILSFNSPLPTLGEVTYSIPEDETVISKPTNEHVENIEEAARVRRSDSIKSDHIRTSVRHRSSTLSIWSLAEEPKQVSLPAWMFLRLLPFYTDDNPIKDSVNIANNSRDFANRKPILPICLKRYEFNNSTAIRSSKRIIIPPVINLPNFVVDDIDDDTAGFKLILESAICHRGNSISSGHFISVVRKDSDTMLDVSEEEAYAAKWYLYDDMRKLHRVVEKSFSEVFSTEWPYMLFYRLVAIEKDKNPFAGAMSENPFVPKGAQGGYWDESLSVTASAESVPAHTTAHTNFSIPIPDVPATDPKFVDVRDKYYWYVLDGDKNYYKEELTSEKGSSSIGLSPQFRRNSQWSTNSNISSIKLDKVDEEKEKENLEAFPVINSNNSDETIWQKIKRTGASTKVDSTESLGEIVSEKTGSQVAFTELADNGDTDRKKKRHFHHRDRKKSVSYQKEKCVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANS5</accession>
    <name>G3ANS5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_50613</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FMP52 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375286.1">
        <property type="nucleotide sequence ID" value="XM_007375224.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANS5"/>
    <dbReference type="STRING" id="619300.G3ANS5"/>
    <dbReference type="GeneID" id="18871462"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50613"/>
    <dbReference type="eggNOG" id="KOG4039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071330_2_2_1"/>
    <dbReference type="InParanoid" id="G3ANS5"/>
    <dbReference type="OrthoDB" id="430436at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051170">
        <property type="term" value="P:import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014843">
        <property type="entry name" value="Him1/Fmp52"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14097">
        <property type="entry name" value="OXIDOREDUCTASE HTATIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14097:SF7">
        <property type="entry name" value="OXIDOREDUCTASE HTATIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08732">
        <property type="entry name" value="HIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="27788" checksum="AF46AB76F622E874" modified="2011-11-16" version="1">MSAFILGSTGLIGGQIVRYLANSPTYKTLYPITRRDDFRLDSRITNLVDKNPDNWPDFISSHPEVTTLISALGTREVSRNSRKAKVLNKVDYGINYSIARVAKENGVKTLILISRENANPYSLSDFVRTKGELERDLKNLGFEHTVIIRVRMVFPRKEGQIGWQNTLGEWLSRMSRNPLVLRSGNYPCMDVDVARIVVDFAEKAESGQLENRVTVVEPWEIVDMADQLIDTAEKLADIPEDLSSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANT1</accession>
    <name>G3ANT1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA polymerase II transcription factor B subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">RNA polymerase II transcription factor B 38 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">RNA polymerase II transcription factor B p38 subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_55579</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375292.1">
        <property type="nucleotide sequence ID" value="XM_007375230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANT1"/>
    <dbReference type="FunCoup" id="G3ANT1">
        <property type="interactions" value="535"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANT1"/>
    <dbReference type="GeneID" id="18871950"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55579"/>
    <dbReference type="eggNOG" id="KOG3800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048466_1_1_1"/>
    <dbReference type="InParanoid" id="G3ANT1"/>
    <dbReference type="OMA" id="PNKRDYY"/>
    <dbReference type="OrthoDB" id="5963at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070985">
        <property type="term" value="C:transcription factor TFIIK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061575">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001174">
        <property type="term" value="P:transcriptional start site selection at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16573">
        <property type="entry name" value="RING-HC_TFB3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000037">
        <property type="entry name" value="Cdk-activating kinase assembly factor MAT1, centre"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004575">
        <property type="entry name" value="MAT1/Tfb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015877">
        <property type="entry name" value="MAT1_centre"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00570">
        <property type="entry name" value="cdk7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12683">
        <property type="entry name" value="CDK-ACTIVATING KINASE ASSEMBLY FACTOR MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12683:SF13">
        <property type="entry name" value="CDK-ACTIVATING KINASE ASSEMBLY FACTOR MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06391">
        <property type="entry name" value="MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17121">
        <property type="entry name" value="zf-C3HC4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="2"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="91"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="330" mass="38909" checksum="F9E5B9ED20174B49" modified="2011-11-16" version="1">MCPICKTDKYLSPNMTFLINPECYHKICESCVDRIFSLGPAPCPYPKCGKILRKNKFKQQVFDDLRIEREIDIRKKVGNVYNKTEEDFPSLKEYNQYLENIEEIVFKLNNDVDVVETLAELDKYEQEHKLEILEKNMRESEKSADLVKYQEAMERLKQEKRKIQEKMDLEDLNFQREQQQKLLDKMATSSETTEEILKQLEVQKQSRLTSRRKQLQSINENLDRQFNSANPFHKHLPEEVQTPFTPFKGDRDLNKKYTMLPVLDAEEIMDTDNSHGSYYDPYMNKLAKVKEFLGAGWRLNHVYERALDEAFIGLGCIIEQEKTSAKPTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANT4</accession>
    <name>G3ANT4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RNA polymerase II-associated protein RBA50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_61121</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RPAP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375295.1">
        <property type="nucleotide sequence ID" value="XM_007375233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANT4"/>
    <dbReference type="FunCoup" id="G3ANT4">
        <property type="interactions" value="174"/>
    </dbReference>
    <dbReference type="GeneID" id="18873725"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61121"/>
    <dbReference type="eggNOG" id="KOG1894">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031074_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANT4"/>
    <dbReference type="OMA" id="TQRCIAI"/>
    <dbReference type="OrthoDB" id="348201at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039913">
        <property type="entry name" value="RPAP1/Rba50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013929">
        <property type="entry name" value="RPAP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013930">
        <property type="entry name" value="RPAP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21483">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21483:SF18">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08620">
        <property type="entry name" value="RPAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08621">
        <property type="entry name" value="RPAP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RPAP1 N-terminal" evidence="4">
        <location>
            <begin position="71"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="RPAP1 C-terminal" evidence="3">
        <location>
            <begin position="293"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="116"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="454" mass="51993" checksum="990976F7B88BDBAC" modified="2011-11-16" version="1">MDFLGEIVEHETETPQAPVPVSTGGFPELGKLKQKKVSRWKQRLDKTKSEPPADQVQEQTQPEVQVLSEAEKIHRENLETMSKMTQQEILAEQQELLEGLDPKLVQSLLSRTKKRSDHDHDHKHEHRHEHAEGYNGWIGAVRTPQGLSDLSQLDKEDVDKALGISHDLPIEDIKERDPSKKSVSFDQTVKTVRYEDLDESVELDPNGWEDVTDINELIPDEIAPEEYQINPEEPQVSVHFPKPKSDDLDINDPDFDTKLHEKYYPDLPIETEKLSWMKPAPETVVTTYESISDMRFDFKGDIVELRDSDDKQDIPTYMGLHHHSENPNLAGYTLSELAHLSRSVLPGQRCIAIQTLGRILYKLGSHHYSIAKTDNEQFDEQVQEMANNFESMIWDLIEELRIVDSVTEASDESKTRNLSVRNYAIEALWLWKKGGGRPKAALKTEEDVVAEALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANZ0</accession>
    <name>G3ANZ0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_138295</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375891.1">
        <property type="nucleotide sequence ID" value="XM_007375829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANZ0"/>
    <dbReference type="GeneID" id="18870111"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138295"/>
    <dbReference type="eggNOG" id="ENOG502S2V3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1120501_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANZ0"/>
    <dbReference type="OMA" id="YDDLYHP"/>
    <dbReference type="OrthoDB" id="4083086at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="259" mass="30768" checksum="B6119894B38D5EE3" modified="2011-11-16" version="1">MSEPSEKDLDIQRRPNPKGWVPPRAPYNPYDPWDIRPPEGYPSEFNAPRKSPKGFSSVPAENTQYGKVNDTMNRLNYSPRPMSDLYPGQYKVLRRVDTNRRLYLGSRYFGLFITVSAFTYAAFFYRWNGGSENVFSEFYRSRLRLKEKYVGLNEEEHDDLYHQKDSGVRIKGVKDSQYIPDSLRKTPENDLVLSRPSEKHILEAERIQQQEEERMLKELDYHEKYLQEYMQKNPIVNEEQPQELLDATRKPRKKWLGIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP05</accession>
    <name>G3AP05_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_138981</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375312.1">
        <property type="nucleotide sequence ID" value="XM_007375250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP05"/>
    <dbReference type="FunCoup" id="G3AP05">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP05"/>
    <dbReference type="GeneID" id="18870165"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138981"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_22_1_1"/>
    <dbReference type="InParanoid" id="G3AP05"/>
    <dbReference type="OMA" id="GIIIFEI"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17502">
        <property type="entry name" value="MFS_Azr1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF198">
        <property type="entry name" value="AZOLE RESISTANCE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="304"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="340"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="370"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="394"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="519"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="45"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="545"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="554"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="566"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="583" mass="63125" checksum="8E54F0EF72D1502E" modified="2011-11-16" version="1">MSSTNQNPHDPEQQSSPSHKYIEADAGDGTRAHEQYLSGISLALTLASCITSLFLVALDQTIVSTVLTTVGDQFNGFDKVGWLTAGFMLPMACLAPSYGKISIAFGRKWTLFVGIIIFETGSLICALSKSMPMLIGGRVIQGVGGGAIQATVMVILTECVPISRRPLVFASISIVYSTSSVLGPLIGGALTKVSWRWCFFINLPIGGVALAVLTFGFKPPKTVGSMREKLAKIDYVGTFLLASGLVLVLLALTFGGIEYPWRSAAIIVLFTLGGLVIITFFIWNFNYSKNPIILKDFLYINRVLAANLSSMFNFAFFIANLTYLALYFQVIFNASALQSGIYLLPMVISVCVSSFCNSMFISITKLIKPTMVLSGLLSPVGTGLFLLLGKSSSLGLRIGILIVCGISIGLQFQSSLLSAQLVSPKSIPGALILVTVFQNFLKNLGATVAVNIAQLLFQTTGTDYINNLTESLPRDSMDHQVLMKFTAKELIANPRLIQYLPDSTRELLLDQYMKAFKNVFYWGLALGSVCFVCSLFTTNKRLPNLKDIDRGDQTPDEDDDEKENESGGSRTGSETSSGTVSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP27</accession>
    <name>G3AP27_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_66724</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375334.1">
        <property type="nucleotide sequence ID" value="XM_007375272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP27"/>
    <dbReference type="GeneID" id="18875114"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66724"/>
    <dbReference type="HOGENOM" id="CLU_855724_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP27"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="325" mass="37852" checksum="93A9DF2CE18AA031" modified="2011-11-16" version="1">MSSDQNQYFEINKNLVEQFMEFIINSGRETKSNIKSSVYKNCESIIKDFSSLKRYKSQNSDRIEHWEISYNKFRITEPELFNALCEYQTFDRAPKQKMEGIIYTGEDLKVYVEILKKILDKIILKKIQGSGYEEEKDIFNTMRNVVKTDSKNFPHIRLENAIRKVDAKARDFLSSSSLDDAFVKMRSLRSTGPDPAYNFIIKFNDFFEYILMLNWMIQTKLVHVAANVLLPTYELHLQQIEDGKVPTAYMKPNEFAETVMRQSEFINLTSRGRPLNINLLQAGKSLSTKPIVNGLNNLDRSDKNATGKKKRNRNMKYLSNVKGDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP37</accession>
    <name>G3AP37_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Topoisomerase 1-associated factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_50663</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the timeless family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375344.1">
        <property type="nucleotide sequence ID" value="XM_007375282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP37"/>
    <dbReference type="FunCoup" id="G3AP37">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP37"/>
    <dbReference type="GeneID" id="18871469"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50663"/>
    <dbReference type="eggNOG" id="KOG1974">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008440_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP37"/>
    <dbReference type="OMA" id="VNHHRHT"/>
    <dbReference type="OrthoDB" id="310853at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031298">
        <property type="term" value="C:replication fork protection complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043111">
        <property type="term" value="P:replication fork arrest"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044998">
        <property type="entry name" value="Timeless"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006906">
        <property type="entry name" value="Timeless_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940:SF4">
        <property type="entry name" value="PROTEIN TIMELESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940">
        <property type="entry name" value="TIMEOUT/TIMELESS-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04821">
        <property type="entry name" value="TIMELESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0236">DNA replication inhibitor</keyword>
    <keyword evidence="8" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Timeless N-terminal" evidence="11">
        <location>
            <begin position="84"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="978"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1076"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="980"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1085"/>
            <end position="1096"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1152" mass="130933" checksum="D3022076CC9019F1" modified="2011-11-16" version="1">MSSSSDSETYEELGDFIVDEEPIQESNSQDESNEPDLANDRIRSLTTTLQPNQTPAQKLLRAHIAVLCSALGGPDHTSNITPPPYKLGHDALACLKDIKRWIRAVDERQNNYAVALACADSGLVKDLIVIMCQFDKKKTGIKVEKIRLACLELLVLLTWPVEFGAELSESQRLLFTEVRKVQVGYKKLILSYLNGAVLKSVIRLVLPVIKKSRLDREPRENQILKLVLYLVRNVVAIVPANASVSSKSSHRAVVVADDLPNGVSADDISINNVVSVFEKNKVLMLLLTISGSIGTEFDKDMFGEICLECIYLLVKGLSVSELLEKPTPKDPTNPSQPLQSVSTTAGMELQDLLASETKRKQSQTATVSTRHGRFGSLLSIKAPDSNPYVVSGQEALFTTSSTLTKLDKSKTWKNRTLFKYDSDEYVISRYPVYLTTTGRNILHEFVKSFLSGGCFNNLIETMSSRLTTISDMTVVDEYTKASYFFTISWFLSYEKQSIKLQQAQDYGSVGAALSSVNFILLTSYFRDSFGSKSWNSLHVAMLCFRELIQISNSLFGKQDADETDRDLAEGIIRKLFSFSDFVNIIAQIPQTAAKHSPAYLGVVVSVVNTLLESFETFANEDVELYIQSRRKKSKSKRNTERHPEALQDVIDASDDEQDAATAKEITKERKLDFKLTEARFFHTSIVNAYIAYLSRYDDLDASQIKICFKYFHRLFVVRKDFTGLYRLDFMQLLERLHHDLRGSSRARVDEFIYYFMKKFKIALERFPMPIELLFPRMEDLSCKIYLATGELYTKPEPNTRGPRLAKQLEFVRDFNRDEQVKILVSALKREDKLALLVWIISELERILSERILDDSSIAELNPGVHRRLFINNGNLRLLLKLIGFDLPFTMDENPELPTSVETATLTEFIDLIKKWKDQHGEFDDGKEPSFFLRAREDDEEEDYGEGDDIAFETAPGSGERMNIHELDALDELEQSLSQRSKGHARKKRPQRVHEHHRTSGERQRLPPVTRTFNSAEFVHDSDDESDDEKDAEFFAREERLRTLLGESGGIVDGRQLAEFKLRWAEIEKNGGVLPERRVDTSELFVNDDDDDDDDIMSDAPQSSTDSSKRSADEEEEEERQEVAPAKRLADDDDEEIAHVKRKRIVVSDDEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP40</accession>
    <name>G3AP40_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_61156</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375347.1">
        <property type="nucleotide sequence ID" value="XM_007375285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP40"/>
    <dbReference type="GeneID" id="18873748"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61156"/>
    <dbReference type="HOGENOM" id="CLU_2293457_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP40"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="11610" checksum="23EC932D6358D3CE" modified="2011-11-16" version="1">MSQSSRDMLDQGIDHHLDINSGTITCLFHGCGVTLDNMDAARRHIEEHGSLVKDLFRKCVNCHKQYNKIDSLLAHMIFQCDEKSSHQINCSCTEYWLEINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP63</accession>
    <name>G3AP63_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Eighth largest subunit of RSC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_139133</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375910.1">
        <property type="nucleotide sequence ID" value="XM_007375848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP63"/>
    <dbReference type="FunCoup" id="G3AP63">
        <property type="interactions" value="527"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP63"/>
    <dbReference type="GeneID" id="18870182"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139133"/>
    <dbReference type="eggNOG" id="KOG1279">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004447_3_2_1"/>
    <dbReference type="InParanoid" id="G3AP63"/>
    <dbReference type="OMA" id="PSFSKWF"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02336">
        <property type="entry name" value="ZZ_RSC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000020">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041984">
        <property type="entry name" value="Rsc8/Ssr1/Ssr2_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802:SF150">
        <property type="entry name" value="CHROMATIN STRUCTURE-REMODELING COMPLEX PROTEIN RSC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802">
        <property type="entry name" value="SWI/SNF COMPLEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SWIRM" evidence="8">
        <location>
            <begin position="68"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="317"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="9">
        <location>
            <begin position="320"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="526"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="458"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="532"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="557"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="569" mass="65046" checksum="2ABE13BE254FF9BB" modified="2011-11-16" version="1">MSSIDPESTPAVDTPPRAATEEPVASSVDEVTPAASSLEPKVDIDVEKLKAEFQERAKTYLAEQTSHVVIPSFAKWFDLNTVHTIEKKAFPDFFSTDPKNVYKTPQSYKYIRDFLVNTFRLNPKEYLTITAARRNLAGDVTNIIRIHQFLEKWGIINYQIDPRSKPSIVGPQYTGHFQLTLDTPQGLVPYVPENAVLIKSEPEEGEVTNKEIETSTKPAQPSEVSIKDELDLNLEIRRNVFGTGEKKSNFKTNNLVQYACSVCGKDATEVRYHNLKIKTYTYNPSSTINNASILCTLCYEQGLFPSNFQSSDFIQLKKNQEAEEWTEQEILLLLEGIEMFGSFDLPNINGNIHANANSQWEKISEHVGTKTREQCIIKFIQLPIEDKFLTKLIKGNDEDNADDVTTKETLVQEVVQKLIETKPGQDLLKHNSNQNLQESILEEANLINQVIELSLEKFNLKLNKLDKLQESLINIENQLNLERKQVLIERWVQFQKIQTLKREKPELADILDDLIQPVRVNEINKSLNPIKNSEKNDDMQIDQNETKENDDDKLPISVSQPKTYQYWSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>G3API6</accession>
    <name>G3API6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar protein sorting-associated protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_61240</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375433.1">
        <property type="nucleotide sequence ID" value="XM_007375371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3API6"/>
    <dbReference type="FunCoup" id="G3API6">
        <property type="interactions" value="1016"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3API6"/>
    <dbReference type="GeneID" id="18873793"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61240"/>
    <dbReference type="eggNOG" id="KOG0739">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_21_2_1"/>
    <dbReference type="InParanoid" id="G3API6"/>
    <dbReference type="OMA" id="IEWTNEF"/>
    <dbReference type="OrthoDB" id="29072at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990621">
        <property type="term" value="C:ESCRT IV complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070676">
        <property type="term" value="P:intralumenal vesicle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090148">
        <property type="term" value="P:membrane fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036258">
        <property type="term" value="P:multivesicular body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02678">
        <property type="entry name" value="MIT_VPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19521">
        <property type="entry name" value="RecA-like_VPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000043">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000015">
        <property type="entry name" value="vacuolar protein sorting-associated protein 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000014">
        <property type="entry name" value="Vacuolar protein-sorting-associated protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045253">
        <property type="entry name" value="VPS4_MIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF83">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04212">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIT" evidence="11">
        <location>
            <begin position="4"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="164"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="74"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="80"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="435" mass="47917" checksum="3A4DF930F4C6D568" modified="2011-11-16" version="1">MSGASDFLSKGIDLVQKAIDADTATRYEEAYKLYYNGLDYLMLAIKYEKNPKSKELVKSKFTEYLTRAEQLKDHLEKQQNKSNSAESSSNGSTKAAKKDGAGGGDEDDADTKKLRGALAGAILSEKPNVSWSDIAGLDGAKEALKEAVILPVKFPQLFVGNRKPTSGILLYGPPGTGKSYLAKAVATEANSTFFSVSSSDLVSKWMGESERLVKQLFTMARENKPSIIFIDEVDALCGPRGEGESEASRRIKTELLVQMNGVGNDSQGVLVLGATNIPWQLDAAVRRRFERRIYIPLPDVEARTRMFEINIGEVPCECTPHDLRTLAEMTDGYSGHDVAVCVRDALMQPIRKIQQATHFKPVIDEDGKERLTPCSPGDEGAREMNWMEIGTDELKEPPLTVKDFIKAVKNNRPTVNEADIAQHVKFTDDFGQEGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3APJ3</accession>
    <name>G3APJ3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Centractin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Actin-like protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Actin-related protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_139807</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the actin family. ARP1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375440.1">
        <property type="nucleotide sequence ID" value="XM_007375378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APJ3"/>
    <dbReference type="STRING" id="619300.G3APJ3"/>
    <dbReference type="GeneID" id="18870240"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139807"/>
    <dbReference type="eggNOG" id="KOG0676">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027965_0_2_1"/>
    <dbReference type="InParanoid" id="G3APJ3"/>
    <dbReference type="OMA" id="YTTWTGG"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10216">
        <property type="entry name" value="ASKHA_NBD_Arp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000007">
        <property type="entry name" value="Actin like 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000018">
        <property type="entry name" value="Actin-like protein (Centractin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00073387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00076361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00083222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="413" mass="46463" checksum="CBA021EB8BC614FD" modified="2011-11-16" version="1">MEGNLYNQPVVIDNGSGNIKAGFAGEDQPKTYASAIVGRPKYQKIMAGSLLMSDEPTTKEEVFVGDVAQQNRGLLKLSYPIEHGVVTNWADMEKLWYNVYTQDLKTNAEEHPLLITEAPLNPRNNRNKMCQILFESFNVPCIYVSIQAVLSLYASGRTTGVVIDSGDGVSHVVPVYEGFSLPSSIKRMDVAGRDITEHLSFNLRRMSGINLQSSSELEIVRLIKESSCFISKDPTRDEKLYRVHYRTNAPGDSSDLFASYKLPDGHNIQLGVERFRAPEILFNPQLIGNESPGIHELTSLAISKTDMDLRPILYQNIILSGGNTLLKNFGDRLLKELKQLQHQQDSSNNTIWNKDRDASYDTKMKLKIFAPPERKYSTWIGGSILAGLSTFKKMWITSQEYHENPDIIHTKCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ07</accession>
    <name>G3AQ07_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_61406</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375604.1">
        <property type="nucleotide sequence ID" value="XM_007375542.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ07"/>
    <dbReference type="GeneID" id="18873877"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61406"/>
    <dbReference type="HOGENOM" id="CLU_2838338_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQ07"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="66" mass="7484" checksum="589A2DBB36E52F50" modified="2011-11-16" version="1" fragment="single">MCKRKLRKKLLCNIIGGYVSAKDIKKLDPPLPFSFEATLEIQFIPLFKAVDFEPPCCGVCQKNLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQB9</accession>
    <name>G3AQB9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Deoxyhypusine synthase</fullName>
            <ecNumber evidence="6">2.5.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_62037</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[eIF5A protein]-L-lysine + spermidine = [eIF5A protein]-deoxyhypusine + propane-1,3-diamine</text>
            <dbReference type="Rhea" id="RHEA:33299"/>
            <dbReference type="Rhea" id="RHEA-COMP:10143"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57484"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="EC" id="2.5.1.46"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the deoxyhypusine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.46" evidence="6"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376244.1">
        <property type="nucleotide sequence ID" value="XM_007376182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQB9"/>
    <dbReference type="FunCoup" id="G3AQB9">
        <property type="interactions" value="709"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQB9"/>
    <dbReference type="GeneID" id="18874160"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62037"/>
    <dbReference type="eggNOG" id="KOG2924">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039781_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQB9"/>
    <dbReference type="OMA" id="HSIINAN"/>
    <dbReference type="OrthoDB" id="294378at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034038">
        <property type="term" value="F:deoxyhypusine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.910.10:FF:000003">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.910.10">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002773">
        <property type="entry name" value="Deoxyhypusine_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036982">
        <property type="entry name" value="Deoxyhypusine_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00321">
        <property type="entry name" value="dhys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703:SF0">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01916">
        <property type="entry name" value="DS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW31466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="378" mass="41400" checksum="6487431252BA90EA" modified="2011-11-16" version="1">MSGSGKLPELASDAVLKQSIPVPEDFVEVKGIDYGKDNAYNMRAKDLINSMSTMGFQASNLSQACDIIDKMRTWRGKHKSELEEHQLTGEFDDQGFQKTTIFMGYTSNLISSGLRDTLRYLVQHKMVSAIVATAGGIEEDLIKVLAPTYMGEFSLPGKGLRDQGMNRIGNLLVPNDNYCKFEEWIVPILDTCLEEQEAAMKELGPEGLNADSAAVWTPSKLIDRLGKEINDESSVLYWAHKNKIPVFCPALTDGSIGDMLFFHTFKASPQQLRLDIVADIRKINSMSMAASQAGMIILGGGLIKHHICNACLMRNGADYAVYINTGQEFDGSDAGARPDEAVSWGKIKADAKSVKVYADVSLVFPLIVAATFAAEKPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQE3</accession>
    <name>G3AQE3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_154714</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376268.1">
        <property type="nucleotide sequence ID" value="XM_007376206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQE3"/>
    <dbReference type="GeneID" id="18870997"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_154714"/>
    <dbReference type="eggNOG" id="ENOG502RY5P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035993_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQE3"/>
    <dbReference type="OMA" id="FCMLKPK"/>
    <dbReference type="OrthoDB" id="4024574at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442554" evidence="1">
        <location>
            <begin position="26"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="423" mass="49714" checksum="AB2640961CCFCA21" modified="2011-11-16" version="1">MKDILPRILISFCLLILVGPWHVSSHRTCHPGRNNHGVPFTGFKITRQYKVTGGILPVSMHFLTLSHCDHGRVDYQNTIRTDWSGYVDWERPFCMLKPKLGEMLTLPVHDTVSERGRQMQDNIELDTYSDIPGFFNVKMRKLRNLLRKKVDINMEKLTNGKFVEYSGKNKFFHHEDLLSSDPQSSLEWESRNLVCYKMARRKKYAKRHISFYVPYTFVGGLFECFIPPQIKREIFQKYTSADLIQQFDFKCDPQLSRPISPLIAEDSTKRWVENKLYNLLNPGIIQTIPYLTTSTPMDLRFSSLETSDMYRDTWATKIDVNEDYVYREADLHIISQAVAKSADLLNKIISPIDFNMLLKAYNRANEREHPDAKSVLEYDPEEFEIMLSKASFDQKVGFWLSYLDEKVSSWYLRKIEHFWDKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQK3</accession>
    <name>G3AQK3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nitrogen permease regulator 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_56377</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NPR2 family.</text>
    </comment>
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        <property type="protein sequence ID" value="EGW31550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376328.1">
        <property type="nucleotide sequence ID" value="XM_007376266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQK3"/>
    <dbReference type="FunCoup" id="G3AQK3">
        <property type="interactions" value="206"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQK3"/>
    <dbReference type="GeneID" id="18872027"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56377"/>
    <dbReference type="eggNOG" id="KOG3789">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014995_3_0_1"/>
    <dbReference type="InParanoid" id="G3AQK3"/>
    <dbReference type="OMA" id="IDVNWDP"/>
    <dbReference type="OrthoDB" id="338854at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015824">
        <property type="term" value="P:proline transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000785">
        <property type="term" value="P:regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015840">
        <property type="term" value="P:urea transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009348">
        <property type="entry name" value="NPR2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12991:SF10">
        <property type="entry name" value="GATOR COMPLEX PROTEIN NPRL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12991">
        <property type="entry name" value="NITROGEN PERMEASE REGULATOR 2/TUMOR SUPPRESSOR CANDIDATE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06218">
        <property type="entry name" value="NPR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="589"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="589"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="615"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="635"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="700" mass="78933" checksum="E7B525FD768436D9" modified="2011-11-16" version="1">MDVSDGFIPIVAVFYAVFHPTEGTKIIHQVPENSIVTTSHQRTKVVKNVSRLSMDSTNSDSRQRQEDEDEDDGLFNFDTVKNYVIPKPQLCNRLISFKINKYKVIGYPVNMENPRYSRNSFSFNFCFVFPYDKGDISPYEPAIKRMGQMFQVLEEQNFMLSKLDKDNSFFKKGSTGLSSNGASSDISNNINIGIDLESYTNATSMSPAASNDATVNAPGHSKTKRISLSSIESLVHQIYQDLNNYSECCIPLDSSNSVDIKLFPILPAPINLKAYQVPIATVKLNSLVDVNWDPTMVKILPFINGLNSVKRISELANANYLLTKQCIQHLMHYKCIEIIDIFQFSNIYAPTNHIGDFLKINGKMAEECQAYVVTSESNIEGSSYFSHTPSTYHNNNTSSFHGSNSVSPYSTAGLKYDSVSSSYPKPSSSYLSKSPKTVGNSPNISVNVKVPTKTTLFYLYRSLNQGQTVKEWYIQHRKLLTSIDIRRFINFGVLRGIIYRVHSYPLVNSLTRSIESGDLAQYDEFLGSYKEKKSKKKVKVKHTDESLLKTKVNEQTIKTDNKRKVSFSYSQSNLHDARKSGFSSREVILENDSEDEDEEEEEGEGHLVHPRSRTISRNSFASSTGLKYASGGEENAIEDSSDDDNEVSEEKEQEKAQLLDLVKMLKGYQSFDSICTELQKSRFEVEKMIEKLGSYNSVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQW0</accession>
    <name>G3AQW0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LsmAD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_51223</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375967.1">
        <property type="nucleotide sequence ID" value="XM_007375905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQW0"/>
    <dbReference type="FunCoup" id="G3AQW0">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQW0"/>
    <dbReference type="GeneID" id="18871536"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51223"/>
    <dbReference type="eggNOG" id="KOG2375">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009985_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQW0"/>
    <dbReference type="OMA" id="YFTAPTW"/>
    <dbReference type="OrthoDB" id="2275718at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034063">
        <property type="term" value="P:stress granule assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045117">
        <property type="entry name" value="ATXN2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009604">
        <property type="entry name" value="LsmAD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025852">
        <property type="entry name" value="SM_dom_ATX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12854">
        <property type="entry name" value="ATAXIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12854:SF7">
        <property type="entry name" value="ATAXIN-2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06741">
        <property type="entry name" value="LsmAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14438">
        <property type="entry name" value="SM-ATX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01272">
        <property type="entry name" value="LsmAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LsmAD" evidence="2">
        <location>
            <begin position="242"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="512"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="833"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="451"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="512"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="559"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="593"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="840"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="858"/>
            <end position="872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="872" mass="93575" checksum="DD270309198D1C78" modified="2011-11-16" version="1">MKGQINRANSTSSSSSTTTTTPKNGYRRTSRYGNGNGSPNITSPTTPPTNASNGTSGDDSLGNNRLLYLVSKSIGKKCIATITDGSRYQGLLVNADLSPTGTSAMSVVLLKPQLVSKSLLNEKNNLDVGKLPENLIIQAKDLIDLEITDLDLTEKVESTTAQQPEPTPVQTVDLTTPPPPPPVVKEVSPSKFKTDVDISGKLQIKERELQRWVPDESIDHPQFSLEDDAGEWDQFKVNQERFGVESTYDEHLYTTRIDTSAPDYHERVARAEQIARDIETSTTTDRHILEERGVMVDDSGMDEEDKYSGVARGGEELMAALRNATISAGPSTLNKSSPGKYVPPRQRAAHYHNDPAIISSSATNRKVVSPPPNSEIPPVSKVETPPVDEKPQSPAQATPVAAASSTKETTTPVPVDTPISRTSTSTSTPDIKKPSPPKKTQPDSIPPKPQVSAQHNESFRLNAQSEINSLREFSANFKIPHKMPQDLLPILSKDKLKQDEILKKQQQVAAAAAVAAATATTTSSIAATPSASQESVSPPEKKKETSKTFKLNPKAAAFTPSTKHAQLTSPPKTNFRSPINDPSPRASQSRPYSSNGSISSQGTSKRHHQISPAEFFGGNDKIPTRESQEKKINAFKFAFSLFITTKDKHEGTGPVVFEKTFQTPPTWDSTIDETHEKLFPQIPPKVATGAPGAPGMILPVASPFIPNPMMAGGAPGIPAGAAGQYPNKFHMTPQQQQQQQQAAAAAAAAAMAVQFQQQQQMHAAMLYQQQHPQAQQQFPVPPPAPGQPPLMYPGAEPFLPPGGFIPPPGAGFMSTGSPVNGNIIMGQYGGANQQQQHNYNNHHHSGGNRRYSGHSKRGGGSTSGASGGSGHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQY1</accession>
    <name>G3AQY1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_56102</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375988.1">
        <property type="nucleotide sequence ID" value="XM_007375926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQY1"/>
    <dbReference type="FunCoup" id="G3AQY1">
        <property type="interactions" value="261"/>
    </dbReference>
    <dbReference type="GeneID" id="18871998"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56102"/>
    <dbReference type="eggNOG" id="ENOG502S51X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077704_1_0_1"/>
    <dbReference type="InParanoid" id="G3AQY1"/>
    <dbReference type="OMA" id="IRSKDRW"/>
    <dbReference type="OrthoDB" id="4096107at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013268">
        <property type="entry name" value="UTP16"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08297">
        <property type="entry name" value="U3_snoRNA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="212" mass="24886" checksum="6E4B7268C84C8218" modified="2011-11-16" version="1">MAVTRSQDKAKKIVFEDNEDEEESVEIEARQEEEDKEPESEEESESESESDDEAPEEESTNKTKQEVLAQQKKREQEQKAQKNAERERRRQLDLRNKQQQESKKATKIEEEALPDLLPDDIMDILSTPEPEETQSKIKGKHIRLDEVELDKKKEIEDKLRKIKLQKKMALKKGPVHVQVQKFGKKSEVVPKSENKIIASRDKWLKRKSVNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR87</accession>
    <name>G3AR87_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Crh-like protein</fullName>
            <ecNumber evidence="14">3.2.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">SPAPADRAFT_61834</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the glycosyl hydrolase 16 family. CRH1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.2.-.-" evidence="14"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376040.1">
        <property type="nucleotide sequence ID" value="XM_007375978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR87"/>
    <dbReference type="FunCoup" id="G3AR87">
        <property type="interactions" value="414"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR87"/>
    <dbReference type="GeneID" id="18874073"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61834"/>
    <dbReference type="eggNOG" id="ENOG502QVQI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040459_0_0_1"/>
    <dbReference type="InParanoid" id="G3AR87"/>
    <dbReference type="OMA" id="WNATANQ"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06923">
        <property type="entry name" value="ChtBD1_GH16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02183">
        <property type="entry name" value="GH16_fungal_CRH1_transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000159">
        <property type="entry name" value="Glycosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017168">
        <property type="entry name" value="CHR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF22">
        <property type="entry name" value="GLYCOSIDASE CRH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037299">
        <property type="entry name" value="Glycosidase_CRH1_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442575" description="Crh-like protein" evidence="18">
        <location>
            <begin position="22"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="461"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="19">
        <location>
            <begin position="7"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="352"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="357"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="417"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="437"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="15">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR037299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR037299-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EGW31262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="481" mass="52762" checksum="321C651B8BEAEB11" modified="2011-11-16" version="1">MNSFVTTLWFALTVLLSFVHADDTITCDATTKCPESAPCCSQFGICGTGAYCLGGCDIRYSFNLTSCMPMPRMDSFQYQFDSKDKVEQIEQQYEYLGNSSEADWVFSGWVDYHDDALLVQMPKNTTGTVLSSTKYIWYGKVGATIKTSHDQGVVTAFILFSDVQDEIDYEFVGYDTKNPQSNYYSQGILNYNNAKNSTVNDTFDYYHLYEFDWSETKIDWYVDGEKVRTLQRSDTWNDTTKRFDFPQTPSRIQISLWPGGDPSNGLGTIEWAGGLINWDTPDIQNYGYYYAYIKEFHVETANLIDGVKLDGSSSNSNDYHAFLYNSTAGNQENVYLTNKKTWLGSQDAVGFNPQNDVKPSSSSSSKSVKATTHSKTVVKTSGSKTFTTTEVTTSSETSNNNNNNNNAPPVNAAQAAQKTGTSDNGYNPSAGIGGFVQDTSNTDSADTGSGNGSSGKNAASGLNVGVMGILFSVILGGSLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARA1</accession>
    <name>G3ARA1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_51689</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376486.1">
        <property type="nucleotide sequence ID" value="XM_007376424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARA1"/>
    <dbReference type="FunCoup" id="G3ARA1">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARA1"/>
    <dbReference type="GeneID" id="18871582"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51689"/>
    <dbReference type="eggNOG" id="ENOG502QWPQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076697_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARA1"/>
    <dbReference type="OMA" id="MMIPGRP"/>
    <dbReference type="OrthoDB" id="19619at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024621">
        <property type="entry name" value="Mba1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07961">
        <property type="entry name" value="MBA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="289" mass="32913" checksum="646BE0951663A4F7" modified="2011-11-16" version="1">MVGLRPIHNALSCGALIAPGMKLAPILARSFATTTPKFAQKHVDMADIPIANIGAMADFYVPPRIWSQPMKYWPVLLGRRFLVFASNTYSIVKYKQETGNRLDFNRWKDEGIEKFVKMNKVFAAACNQKVGNLAREKYLVKNLQHECGSQTIARLLERVVTFPLDSTVKWDLVSIEKNPKIVSFNAIPDRNNVVVYVQFIMKLVTKQKMTITTDDKEHVSEVTSTDYLVYTLNPHTKESVLVGKLFESNPVRKVSPDYAQQANPVWLKRFTAETADIYRTDPRLESKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARA6</accession>
    <name>G3ARA6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Alpha-1,2-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_72460</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376491.1">
        <property type="nucleotide sequence ID" value="XM_007376429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARA6"/>
    <dbReference type="STRING" id="619300.G3ARA6"/>
    <dbReference type="GeneID" id="18875437"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72460"/>
    <dbReference type="eggNOG" id="ENOG502SJ0W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013298_1_0_1"/>
    <dbReference type="InParanoid" id="G3ARA6"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646:SF1">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="652" mass="75703" checksum="B595558E63AED441" modified="2011-11-16" version="1">MIISPKALKSIQNEEEYKLVGYHQDRQTNLIVLQKSYLNELDSSSSNQVWNSIKSDIDNNVDFDLKVISGYNYKEMIERINSENELSLNNSVIEQFETEQKFIHASRDFFTKLMQFIQSHKPDIGGINNNEHFPNGKKIEEYYQKQKQVSDEELAKIDTEDLLHKYGHLPVYGGHLREQYSEEVVRTKEYLSLFITLSQQEKDSLRNSHNAFVHNMMTQYSDELLQFNKFNDFMKGDGIVYLGGGSYNQLVLVSLRILRENKSRLPVEVLMPYEQDYDQEFCHHILPTLNAKCKLMTHYLPKSFMDNVGGFQLKNIALLVSSFERVLYIDSDNIPIKNPDSFFVNKPFTNKHLVMWPDLWRRSTSPTFYDIAGIPVDDTFQARNSYAKGDPRGINEDTSKISYHDLKGAIPEASSETGQILINKKVHFQTLILAMYYNYYGPDFFYPLLSQGAAGEGDKETFIAAAHKLNLPYYQVQEFCRELGPVDPNSKKHNIFAMGQYDPIIDYIQTGEDPSVENHAQGSKQHVVDYYSESTLKYPKHMKDTSMSNYDFHYFKSSSLAFLHANWPKLYLHVFSDQDRGPIIKEGERRRLYGMEMLNELSGYDFELQLTRGVYSTFCIKPYLIISKGDHADDRDKICENINAQIQFLEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARJ1</accession>
    <name>G3ARJ1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone deacetylase</fullName>
            <ecNumber evidence="3">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_61884</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase family. HD type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376090.1">
        <property type="nucleotide sequence ID" value="XM_007376028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARJ1"/>
    <dbReference type="FunCoup" id="G3ARJ1">
        <property type="interactions" value="126"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARJ1"/>
    <dbReference type="GeneID" id="18874094"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61884"/>
    <dbReference type="eggNOG" id="KOG1342">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007727_7_6_1"/>
    <dbReference type="InParanoid" id="G3ARJ1"/>
    <dbReference type="OMA" id="QDSISHR"/>
    <dbReference type="OrthoDB" id="1918432at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034967">
        <property type="term" value="C:Set3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045835">
        <property type="term" value="P:negative regulation of meiotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032874">
        <property type="term" value="P:positive regulation of stress-activated MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF36">
        <property type="entry name" value="HISTONE DEACETYLASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037913">
        <property type="entry name" value="His_deacetylse_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="291" mass="32664" checksum="560E0C09C09FD614" modified="2011-11-16" version="1">MYDYSAIYAGASLDATSKLISGMSDIAINWSGGLHHAKKFEPSGFCYVNDIVLSIINLLRVHPRVMYIDIDLHHGDGVQEAFYTTDRVMTVSFHKYNGEFFPGTGSVDEIGLGKGKNYAINVPLRDGIDDESYIRLFKSIIEPLITKFQPTCIVQQCGADSLGYDRLGCFNLNIRAHGECVKFVKSFGIPMLVLGGGGYTPRNVSRLWCYETSVLNDVTLDAKIPNYLPTYQWFGPDYSLHPQLEGRIDNKNSKKYLESITQEVLEQLRYLNHAPSVQMYEIPPDLPGAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARR0</accession>
    <name>G3ARR0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AP complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_72152</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376105.1">
        <property type="nucleotide sequence ID" value="XM_007376043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARR0"/>
    <dbReference type="FunCoup" id="G3ARR0">
        <property type="interactions" value="857"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARR0"/>
    <dbReference type="GeneID" id="18875418"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72152"/>
    <dbReference type="eggNOG" id="KOG1061">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006320_4_0_1"/>
    <dbReference type="InParanoid" id="G3ARR0"/>
    <dbReference type="OMA" id="NPPEVQW"/>
    <dbReference type="OrthoDB" id="10254310at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048203">
        <property type="term" value="P:vesicle targeting, trans-Golgi to endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016342">
        <property type="entry name" value="AP_complex_bsu_1_2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002291">
        <property type="entry name" value="AP_complex_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="8">
        <location>
            <begin position="29"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="702"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="713"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="733" mass="82931" checksum="FEC287E29A7A7A45" modified="2011-11-16" version="1">MSNTLNANLNKVNSILERKLRSFLSGPKKGETFELRNGLVSQYKHERKDSIQRVIQAMTLGKDVSSLFPDVLKNIATYDLQQKKLVYLYLMNYAKTHPELCILAVNTFVQDTEDPNPLIRALAIRTMGCIRVDKMVDYMEIPLQRTLKDENPYVRKTAAVCVAKLFDLNPQICIEFGFLEQLQELIKDSNPMVVVNALNALYEIKDMNTDENLVVFEINHEIIQNLVLCLNECTEWGRITILTTLNDYTTSDVEQVNHIIERVIPQLQHVNPSVVLSSIKVILHHLEIIPSQQQRDGILKKLSAPLVSLVNSSSIPEAQYVGLKNIRIILEKYPQILSKELRIFFLKYSDPLYLKLEKLEIMIRLANESNSTILLSELKEYAMEFEPSLVSKAIKSIGSVAIQLSNCVVKSVTLLNEIIEQRGGDLIINESIIVLTNILRRYPRKNDFITLIIPIISNHIEELDRNDSLSSYIYLLGEYPKYFSNLHDKLTKLVEQFLSFDPILQLNTLTTIVKINLQSTGTQFSSLLQQVLEMSTKECENADVRDKAYIYWRLLSSSSSVEVQKRIVLSKIPAIKTTISSFNPIILETLMSELSTLSSVYHKPAKTFIDASAYSRTPKTNEKLEDLKTKAKKEIINNVKNENLLDFDDDGNENGVGSEEGVNSLLDELNDLFMAQPPPTQSEAPQQGQSQTNDILSLFNTIPTTTPGKPSFTGSSTTTNTGSTVNNDLLDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARV0</accession>
    <name>G3ARV0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cell division control protein 25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_154407</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376145.1">
        <property type="nucleotide sequence ID" value="XM_007376083.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3ARV0">
        <property type="interactions" value="259"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARV0"/>
    <dbReference type="GeneID" id="18870981"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_154407"/>
    <dbReference type="eggNOG" id="KOG3417">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002171_1_0_1"/>
    <dbReference type="InParanoid" id="G3ARV0"/>
    <dbReference type="OMA" id="IIEPKPR"/>
    <dbReference type="OrthoDB" id="546434at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11883">
        <property type="entry name" value="SH3_Sdc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF368">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 4" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="28"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="9">
        <location>
            <begin position="871"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="8">
        <location>
            <begin position="1039"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="131"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="447"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="665"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="137"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="172"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="447"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="668"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1289" mass="147482" checksum="9D85D8FA3673E67A" modified="2011-11-16" version="1">MSNQFSDEIAPDMVGPHDPKSSNNEPLKHLDTVIALYDFSATQPSHLPLNLGDTVYVLSKSNTGWWDGVVISPNGELQRGWFPSNYGRSVNYVQPVLNKLKSNKELDSITAANTAANVLIPSFTNLLQKNLGDSEKNSPSNTSRKNSVVSFASSETSIPSDSKTLSIAEISTPENTPTSQSLQDGLNENDEINPKDVEEAERLAEGYREKYGKSVTWIPKVTSKGDFIFYCQQLDIYCESPPMLVFNPDDITPNFEYPSPENLSDTSIVHVQGSQSAANVLGARANSSTSFDALKRDSHSSMATQSSSSSYHHFNRPFFAMDNLFYDHSTDITHWSDLRKEANNVLDFIARSLRDQNRQLFGTHFSRLNVFISTILSCSRLNQEDFVGTKYRDSIRRKLKRIGTAFSQIYVNAVLHLRVLHNSEETSQGQLFSYDISTLNKLTNVSPPAASSVQGVSESENKDAAPTKTEQGYESYMHQLNFEIENLRSNINSLVKIFIKLSRGKKTKRSDYDSSDASDEEGDDRFDVLPQTKPRFLMDEFNGGNWCNPFFSTNNPALNVSGDDLKNRYHTKIIIDHQAYDSVFQYMEEMTKLSEETLEYLDPKVQEMYYYNATLKNERNTQILRLVYKYLYHASSMIDLIESFDFTVFCLIKRYSSNEGDCLDPMKESSSSLTTNTENNSAEEKASNSKLTFDYPVVLDFFYLKQEFHDLILRIIMSTQSLTLEDPEVFKGLRDEDALFYNRDTLKIPTEKASLLLSNILSEKINNLKGNAISINPDTLLSDYLYDGLKSCDVMLTIIQQLIEERETILNYATRVMHDDFDVQLLVMERNNTISSEKSDDHSYYSGGHKKTNDVPWYLEGDDEYDLLLDVKGNIKGGTKEALVAHLTHHDMFDSNFNAVFLLTFASMMSLGEFISLLIARFNIEAPEGLSYEEYNTWISKKQNPIRLRVMNIMKLLVGKHWSKSYFNVSILRKWFNFAQCAPVQSYSVGRMLTHDLQKLLQGEQIYVEREPVIPDTKPPAPLTRGPILGRKMKLLDIDYVELARQLTLREFRLYCKITKFACLAKVWGKKSGLNESIDDITAFIKASNQLTNFVAYMILRKQDAKKRVQIIRYFIQVAEKCRQYNNFSSMTAIISALYSSPIHRLKKTWKFVTSDSLSHLQNMNKLMNSSRNFNEYRDVLKFIGSEPCVPFFGVYLSDLTFVYHGNPDFLINRTRMINFAKRAKTCEIVTGIDRFKTIGYNFQEVSEIQKYLDSWFDKCPPIEEQYQISLNLEPREQAPNTTNTKRHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARX2</accession>
    <name>G3ARX2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab proteins geranylgeranyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_141384</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Substrate-binding subunit (component A) of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the Rab GDI family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376599.1">
        <property type="nucleotide sequence ID" value="XM_007376537.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARX2"/>
    <dbReference type="FunCoup" id="G3ARX2">
        <property type="interactions" value="196"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARX2"/>
    <dbReference type="GeneID" id="18870371"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141384"/>
    <dbReference type="eggNOG" id="KOG1439">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021695_3_1_1"/>
    <dbReference type="InParanoid" id="G3ARX2"/>
    <dbReference type="OMA" id="HQYLEFQ"/>
    <dbReference type="OrthoDB" id="1923006at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004663">
        <property type="term" value="F:Rab geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.405.10:FF:000003">
        <property type="entry name" value="Rab proteins geranylgeranyltransferase component A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.519.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018203">
        <property type="entry name" value="GDP_dissociation_inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017230">
        <property type="entry name" value="Mrs6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787:SF4">
        <property type="entry name" value="CHM, RAB ESCORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787">
        <property type="entry name" value="RAB GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00996">
        <property type="entry name" value="GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037514">
        <property type="entry name" value="Rab_ger_ger_transf_A_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00891">
        <property type="entry name" value="RABGDIREP"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00894">
        <property type="entry name" value="YEASTMRS6P"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="592"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="626"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00060123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR037514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW31821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="651" mass="73043" checksum="AF9F47F13AD77CD3" modified="2011-11-16" version="1">MATPISSTPTATSSFSKRERRQSMAERRPSTSYTPAVIPHLAGLETPQEMSPLKLEECDVVIIGTGLSQSILAAALAWQGTQVLHIDNNHYYGDSSSCLTIDQLKKWCVDVNQGKFNHFKDAQIYIPGGKRSNKYTSRDYGIDLTPKIMFCQSDLLSLLIQSRVYRYLEFQSLANFHVFENDDFKQKITNSTSKHDIFTDKTLSLITKRNLMKFLKFILIEEEHVRRALVKQYSNRPIHEFLTQEFKLEESQINELVYSIGLCNKQETTTKEAVIRIRRFLSSFDCYGKFPCMTSKFGGPGEISQGFCRSAAVAGTTYKLSTTMTDFDPVSKTAHFSDGSNIKVNEKIIISPTQIPKFLVPSYQKLTESLPSYYITRLITVVKRDCKEWMDPPQSASAAIVVFPPHSLPTDNESAVQVIIQNGDSGVCPEGQSIWYSHTCEQDLARAKRDLECAFEKMEMALLRGTPGTIPDVLDDIDLFSAGTTPMADSFKLGSSLANFMPKEKVEIVCKVGYVQQTYINPDLSNVFKANPNNSITCKKVPTGVDDEDMIIFGNMPSSELSYDGIITNVKQIYARVTGTDDDFFDVDFEDDDEEMQDHQQPQQPQVNQSGQVLGGIVGGGRMDEDAIDEDVIEDDSDDDHKPFGVDQMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARZ5</accession>
    <name>G3ARZ5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit H</fullName>
            <shortName evidence="4">eIF3h</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_155559</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit H family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376622.1">
        <property type="nucleotide sequence ID" value="XM_007376560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARZ5"/>
    <dbReference type="STRING" id="619300.G3ARZ5"/>
    <dbReference type="GeneID" id="18871021"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_155559"/>
    <dbReference type="eggNOG" id="KOG1560">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044094_1_0_1"/>
    <dbReference type="InParanoid" id="G3ARZ5"/>
    <dbReference type="OMA" id="WYQSTYF"/>
    <dbReference type="OrthoDB" id="10265695at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08065">
        <property type="entry name" value="MPN_eIF3h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03007">
        <property type="entry name" value="eIF3h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027524">
        <property type="entry name" value="eIF3h"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045810">
        <property type="entry name" value="eIF3h_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19445">
        <property type="entry name" value="eIF3h_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="6">
        <location>
            <begin position="11"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="232"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="323" mass="36889" checksum="1EC2CEFE695DFECF" modified="2011-11-16" version="1">MSDQTSSANTITISSSVLLSLIRHTSENYPSLFSGALLGFEDDNGVIDVTHGFPFPYPDQYEGGSLKSRSGAQYQKDILENFKKLGYGIEFQGWFQSTISGNFVTSQLVEALAQQQLANANSFIIIHDMSSIGKEVSLKALRLSQGFMTAYQDGKWKSKDLEHLKLSYFNIFDELNVEVKNQALVNLYLANLKSTEPELDILNLASHQTSTGQLLESLYSQIDSFNYDQNNFNYYQRQLQKEQSKINQWKQQRKLENLERVKKGDKELDTEEWKTLFRLPNEPSRFNNMLHSHAIDVLADDILKKCDEELTKSSVIERKLTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS07</accession>
    <name>G3AS07_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62465</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376634.1">
        <property type="nucleotide sequence ID" value="XM_007376572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS07"/>
    <dbReference type="GeneID" id="18874331"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62465"/>
    <dbReference type="eggNOG" id="ENOG502RQIF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2795584_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS07"/>
    <dbReference type="OrthoDB" id="4023511at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="68" mass="7305" checksum="3D9CEE144A74AD0A" modified="2011-11-16" version="1">MSSDDNKEFGQLGDIAKKFTEGKSNEEIGAEAQEAIGKFEKLSDQDKEGLLKGALGKVEGDKGESKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS16</accession>
    <name>G3AS16_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PQ-loop-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_141086</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376643.1">
        <property type="nucleotide sequence ID" value="XM_007376581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS16"/>
    <dbReference type="GeneID" id="18870341"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141086"/>
    <dbReference type="eggNOG" id="KOG2913">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040201_4_0_1"/>
    <dbReference type="InParanoid" id="G3AS16"/>
    <dbReference type="OMA" id="IDWNGAF"/>
    <dbReference type="OrthoDB" id="407617at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000024">
        <property type="entry name" value="Putative membrane protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF37">
        <property type="entry name" value="PQ-LOOP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="226"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="242"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="28759" checksum="4590964A0DA88525" modified="2011-11-16" version="1">MYNAVAENVLGTIGTVFWCIQLVPQIIRNYKVKDCEGLPPLMLFLWAASGVPFAIYFFATDGSIPLRIQPQLFTFFCLISWIQSLYYPPVAMKRTKLVALVFAFVLVAVGLEVGFILWLRPLYRRGIEWPMLIIGIIASVLLAVGLLPPYFELAKRKGRVIGINFVFLTMDSLGALFSILSVAFGTMDIMSIVLYAIVLGLEIGIFLSHFIWWLRFGRSEQEKEVDYSSDENIENDATVIASHEDDKEKPDTTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS87</accession>
    <name>G3AS87_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA polyadenylation factor FIP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_52228</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FIP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377080.1">
        <property type="nucleotide sequence ID" value="XM_007377018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS87"/>
    <dbReference type="FunCoup" id="G3AS87">
        <property type="interactions" value="235"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AS87"/>
    <dbReference type="GeneID" id="18871648"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52228"/>
    <dbReference type="eggNOG" id="KOG1049">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039307_2_0_1"/>
    <dbReference type="InParanoid" id="G3AS87"/>
    <dbReference type="OMA" id="GFNEYTW"/>
    <dbReference type="OrthoDB" id="10250831at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007854">
        <property type="entry name" value="Fip1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051187">
        <property type="entry name" value="Pre-mRNA_3'-end_processing_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484">
        <property type="entry name" value="FIP1-LIKE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484:SF0">
        <property type="entry name" value="PRE-MRNA 3'-END-PROCESSING FACTOR FIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05182">
        <property type="entry name" value="Fip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-mRNA polyadenylation factor Fip1" evidence="7">
        <location>
            <begin position="139"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="253"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="269"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="279" mass="30219" checksum="FE8F0998F31CFED5" modified="2011-11-16" version="1">MANSDDDDDAFLYGSDEETDGHISKKQKLAADTELDQEETEPKQASPIAEQKEADEEDDDEEDDDEEEEDSEDDIDIIIGETAPKVSSLVPGNDVISEVVDTDAEKDSATTIIVSKDQSESKIDVNAVADYEGKPITSIDLETLKEKPWRAPGVDISDYFNYGFDEFTWTAYCHKQDSTRGEFNPQKVMADIMKGSGGAGPGVPPPPPGMPPMGMLPPGMPPMGMMPPGMQMPPGMQMPPGMPMPPAGMPMPPAGMPPMPNMPNFQFNMPPPPPPPPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASC9</accession>
    <name>G3ASC9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ATP-dependent RNA helicase SUB2</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">ATP-dependent RNA helicase sub2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">SPAPADRAFT_62538</name>
    </gene>
    <organism evidence="25">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">ATP-binding RNA helicase involved in transcription elongation and required for the export of mRNA out of the nucleus. SUB2 also plays a role in pre-mRNA splicing and spliceosome assembly. May be involved in rDNA and telomeric silencing, and maintenance of genome integrity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the DEAD box helicase family. DECD subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376702.1">
        <property type="nucleotide sequence ID" value="XM_007376640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASC9"/>
    <dbReference type="FunCoup" id="G3ASC9">
        <property type="interactions" value="1288"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASC9"/>
    <dbReference type="GeneID" id="18874367"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62538"/>
    <dbReference type="eggNOG" id="KOG0329">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_0_1"/>
    <dbReference type="InParanoid" id="G3ASC9"/>
    <dbReference type="OMA" id="YAHVEPK"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:subtelomeric heterochromatin formation"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
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        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
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    </dbReference>
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    <keyword evidence="12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="23">
        <location>
            <begin position="55"/>
            <end position="83"/>
        </location>
    </feature>
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        <location>
            <begin position="86"/>
            <end position="261"/>
        </location>
    </feature>
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        <location>
            <begin position="273"/>
            <end position="434"/>
        </location>
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        <location>
            <begin position="1"/>
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        </location>
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        <location>
            <begin position="55"/>
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        </location>
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        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
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        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
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    </evidence>
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        <source>
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        <source>
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        </source>
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        </source>
    </evidence>
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        </source>
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    </evidence>
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        <source>
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        </source>
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            <dbReference type="ARBA" id="ARBA00047984"/>
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        <source>
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        </source>
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        <source>
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        <source>
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        </source>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="439" mass="49558" checksum="E4FE75518CAC87AA" modified="2011-11-16" version="1">MSHEGQEELLDYSDSEEIAVPTTSATTAGNDAGAEGAANDKEADKKGSYVGIHATGFRDFLLKPELLRAIGDCGFEHPSEVQQVCIPQSILGTDVLCQAKSGLGKTAVFVLSTLQQLDPIPGEISTLVICHTRELAYQIRNEYARFSKYMPDVKTEVFYGGTPIKRDIEKLKNKDTCPHIVVATPGRLHALVQEKAIRLNNVKSFVIDECDKVLEAIDMRRDVQDIFRATPHQKQVMMFSATLSQEIRPVCKKFMQNPLEIYVDDEAKLTLHGLQQYYIKLEEKEKNRKLSDLLDSLEFNQVIIFVRSTQRANELNKLLCACNFPSIAVHSGLPQEERIERYRSFKEFNKRICVSTDVFGRGIDIERINLAINYDLPNEADQYLHRVGRAGRFGTKGLAISLVSTKEDEEVLEKIQSRFDVKITEFPEEGVDPSTYMNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASP7</accession>
    <name>G3ASP7_SPAPN</name>
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        <recommendedName>
            <fullName evidence="4">Proline-rich protein HUA1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_142630</name>
    </gene>
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        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007377082.1"/>
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    </dbReference>
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    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142630"/>
    <dbReference type="eggNOG" id="ENOG502S12N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078573_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASP7"/>
    <dbReference type="OMA" id="GMVHFLF"/>
    <dbReference type="OrthoDB" id="2405700at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038910">
        <property type="entry name" value="Hua1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28031">
        <property type="entry name" value="PROLINE-RICH PROTEIN HUA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28031:SF1">
        <property type="entry name" value="PROLINE-RICH PROTEIN HUA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="282" mass="30990" checksum="464BFF7BE129AB31" modified="2011-11-16" version="1">MSNFNKDDDLAPADAPPSYAEAVSTQATGMTNGGSSSYSQHSAPPPPAPRPQPPQSSYRPPPPPTRPPQQPPRPQQSSTSSLYTGNTNLPFSFPKGYLCSKCKNTGYKIKHGDVCRTCWDKFYLSKNAYNPSPSLPFKYPRKYYCNKCHNTGYKIKNGKSCQDCWSLFGPRNSYSSVVNGYNPSYFGTTTFLPYSSPSGLRVPPGDPRLGGILCGRCRGSGVTRFLLDVDLCPICNGLGRVITSGTQQHAPMPPRPPQHIPMPPPPPPQPPMQPYYYPPEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASU5</accession>
    <name>G3ASU5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA primase</fullName>
            <ecNumber evidence="10">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_63074</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="10"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377193.1">
        <property type="nucleotide sequence ID" value="XM_007377131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASU5"/>
    <dbReference type="FunCoup" id="G3ASU5">
        <property type="interactions" value="1308"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASU5"/>
    <dbReference type="GeneID" id="18874584"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63074"/>
    <dbReference type="eggNOG" id="KOG2851">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028288_1_0_1"/>
    <dbReference type="InParanoid" id="G3ASU5"/>
    <dbReference type="OMA" id="NVTRGFN"/>
    <dbReference type="OrthoDB" id="9971853at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04860">
        <property type="entry name" value="AE_Prim_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.920.10:FF:000003">
        <property type="entry name" value="DNA primase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.920.10">
        <property type="entry name" value="DNA primase, PRIM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002755">
        <property type="entry name" value="DNA_primase_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014052">
        <property type="entry name" value="DNA_primase_ssu_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00335">
        <property type="entry name" value="primase_sml"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10536">
        <property type="entry name" value="DNA PRIMASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01896">
        <property type="entry name" value="DNA_primase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56747">
        <property type="entry name" value="Prim-pol domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 10" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3 10" id="KW-0639">Primosome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW31160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="448" mass="52012" checksum="6D9EAE848F378050" modified="2011-11-16" version="1">MAPVVEDPAMLQTLNTNATPQSNRPSETDMIFYYQRLLPFRQIFQWLNHSPKPTTDFTKREFAYEYRNGAYQRYNSFGTLDEFKNSVVRANPTRFEIGAIYSIDPKERKNVPKSAMKPRGKELVFDIDLTDYDDIRTCCSGTDICTKCWKFIQVGSKIIESALREDFGFSNLIWVFSGRRGAHCWVSDARARNLDETMRKAIIDYLDVLGAKGIKSSKLSIKKPYHPHIERSFNILKNSFSEVILEEQNPWLTSMDANNEKAWVQIEELLSLIPHKSLQIELRNKWKNSYSSSRSKWDDINKLAKSVLKSNIQVNQLNEAKKDIIIYYMYPRLDVEVSRQVIHLLKSPFCIHPSTGNVCVPFDINKNISGNPENDEYGFNPTNAPNLRQLQNELEQSEVLNEEGEGKHPYEKTSLKPYVEYFTKFVNDLVKQELKGVGKREREDNLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASV0</accession>
    <name>G3ASV0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CCA tRNA nucleotidyltransferase, mitochondrial</fullName>
            <ecNumber evidence="7">2.7.7.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">CCA-adding enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNA CCA-pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA adenylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">tRNA nucleotidyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_142212</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Nucleotidyltransferase that catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs, which is necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded. The structural flexibility of RNA controls the choice between CCA versus CCACCA addition: following the first CCA addition cycle, nucleotide-binding to the active site triggers a clockwise screw motion, producing torque on the RNA. This ejects stable RNAs, whereas unstable RNAs are refolded while bound to the enzyme and subjected to a second CCA catalytic cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14433"/>
            <dbReference type="Rhea" id="RHEA-COMP:10465"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="EC" id="2.7.7.72"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.72" evidence="7"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376765.1">
        <property type="nucleotide sequence ID" value="XM_007376703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASV0"/>
    <dbReference type="FunCoup" id="G3ASV0">
        <property type="interactions" value="132"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASV0"/>
    <dbReference type="GeneID" id="18870431"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142212"/>
    <dbReference type="eggNOG" id="KOG2159">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019592_2_1_1"/>
    <dbReference type="InParanoid" id="G3ASV0"/>
    <dbReference type="OMA" id="ATREDCF"/>
    <dbReference type="OrthoDB" id="445712at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052929">
        <property type="term" value="F:ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052927">
        <property type="term" value="F:CC tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:CCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000019">
        <property type="entry name" value="tRNA nucleotidyltransferase cca2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3090.10">
        <property type="entry name" value="cca-adding enzyme, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032828">
        <property type="entry name" value="PolyA_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13734:SF5">
        <property type="entry name" value="CCA TRNA NUCLEOTIDYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13734">
        <property type="entry name" value="TRNA-NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12627">
        <property type="entry name" value="PolyA_pol_RNAbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81891">
        <property type="entry name" value="Poly A polymerase C-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly A polymerase head" evidence="14">
        <location>
            <begin position="68"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA nucleotidyltransferase/poly(A) polymerase RNA and SrmB- binding" evidence="15">
        <location>
            <begin position="235"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00050431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00082324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF12627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW30732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="564" mass="64907" checksum="486D789FDC0080E3" modified="2011-11-16" version="1">MKRFACSIFYQQRLGSRFLSYQQSSMKRPVCDYITLTAPERRVRELLVEYCNYYNTQNGIGEEDQLELRITGGWVRDKLLGNESHDIDIAVNHLTGEQFAHGLSDYVSKYQPDLDMHTLHTIKKNPDKSKHLETCTTKLYGIDIDFVNLRSEEYSLDSRVPVIEFGTPQEDALRRDATLNALFYNLNHSKIEDFTGKGLEDLHHGLLRTPLPPIQTFLDDPLRIIRLIRFASKFNFMIEQKTLCAMKEEHNKIALSTKISKERIEIELRKILTSKNPGYGLQLINYIGLARSIFYVDSLENEFTKEELKLACDKMPAQLALSTKIYPNFKHIIVNSTKKFRHAFAELLHNEDSMNIFWLAIILQPYSILPPRKPNHDGITKFMKDGLKSKRSDVIKVQAIAQNYKYQTGVIKQLFQDPTTVKRSDLGLYLRQFPGFAPLSLITDCLLDCMHDLHGAIDISSELPPFPESSQEMLNDEHINRVINDNVHKYEELFAAIDQLGLADVHTVKPLLDGTTLSKKLDLKPGPWLKCVNDEILVWQLDNVGATREDCFKFVETIISKYTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT12</accession>
    <name>G3AT12_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sphingolipid delta(4)-desaturase</fullName>
            <ecNumber evidence="8">1.14.19.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_52340</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Delta(4)-fatty-acid desaturase which introduces a double bond at the 4-position in the long-chain base (LCB) of ceramides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>an N-acylsphinganine + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = an N-acylsphing-4-enine + 2 Fe(III)-[cytochrome b5] + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:46544"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:31488"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="EC" id="1.14.19.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the fatty acid desaturase type 1 family. DEGS subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.17" evidence="8"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377206.1">
        <property type="nucleotide sequence ID" value="XM_007377144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT12"/>
    <dbReference type="STRING" id="619300.G3AT12"/>
    <dbReference type="GeneID" id="18871663"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52340"/>
    <dbReference type="eggNOG" id="KOG2987">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032156_0_1_1"/>
    <dbReference type="InParanoid" id="G3AT12"/>
    <dbReference type="OMA" id="GATCNQN"/>
    <dbReference type="OrthoDB" id="1906957at2759"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042284">
        <property type="term" value="F:sphingolipid delta-4 desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03508">
        <property type="entry name" value="Delta4-sphingolipid-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011388">
        <property type="entry name" value="DES1/DES2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013866">
        <property type="entry name" value="Sphingolipid_d4-desaturase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12879">
        <property type="entry name" value="SPHINGOLIPID DELTA 4 DESATURASE/C-4 HYDROXYLASE PROTEIN DES2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12879:SF8">
        <property type="entry name" value="SPHINGOLIPID DELTA(4)-DESATURASE DES1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08557">
        <property type="entry name" value="Lipid_DES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017228">
        <property type="entry name" value="Sphnglp_dlt4_des"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01269">
        <property type="entry name" value="Lipid_DES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Sphingolipid delta4-desaturase N-terminal" evidence="10">
        <location>
            <begin position="25"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW31173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="370" mass="42948" checksum="857F10BE22CBFDB6" modified="2011-11-16" version="1">MTQIKHRNSSHTFTINPPPADAPIEVLNEFYWTKDPEPHIARRKLILEKYPEVRKLNGYEPKTKWYVIAIVGLQVAVAYYLRDSSILSWKFFLLAYVIGATSNQALFLAIHELSHNLLFKKPLHNKLFAIFANIPIGIPYSASFQPYHQLHHKFLGDKYLDTDLPTKFEAIVLSNVLGKIFFATCQIFFYALRPMFITQIKFTYIHLLNVIAQLAVDTLMVKYFGWKSVIYFLMSSFLAGSLHPCAGHFIAEHYVLNDNNVPKVQKTEGANIASGYQLPAETYSYYGSLNLLTWNVGYHNEHHDFPYIAWTKLPELRRIAAEFYDPLPQVTSWCGVIWWFITNDINTLWNRVKREGKEMHGHKIDNLDKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT41</accession>
    <name>G3AT41_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Zf-CHY-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_72723</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376837.1">
        <property type="nucleotide sequence ID" value="XM_007376775.1"/>
    </dbReference>
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    <dbReference type="STRING" id="619300.G3AT41"/>
    <dbReference type="GeneID" id="18875453"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72723"/>
    <dbReference type="eggNOG" id="KOG1940">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013368_7_0_1"/>
    <dbReference type="InParanoid" id="G3AT41"/>
    <dbReference type="OMA" id="CKIECPT"/>
    <dbReference type="OrthoDB" id="411372at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16464">
        <property type="entry name" value="RING-H2_Pirh2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039512">
        <property type="entry name" value="RCHY1_zinc-ribbon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008913">
        <property type="entry name" value="Znf_CHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037274">
        <property type="entry name" value="Znf_CHY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017921">
        <property type="entry name" value="Znf_CTCHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037275">
        <property type="entry name" value="Znf_CTCHY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319:SF0">
        <property type="entry name" value="AND RING FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G08900)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319">
        <property type="entry name" value="RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05495">
        <property type="entry name" value="zf-CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14599">
        <property type="entry name" value="zinc_ribbon_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161219">
        <property type="entry name" value="CHY zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161245">
        <property type="entry name" value="Zinc hairpin stack"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51266">
        <property type="entry name" value="ZF_CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51270">
        <property type="entry name" value="ZF_CTCHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHY-type" evidence="7">
        <location>
            <begin position="369"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="CTCHY-type" evidence="8">
        <location>
            <begin position="440"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="507"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="316"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="662"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="323"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW30804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="735" mass="84812" checksum="974AF3A966C4AE67" modified="2011-11-16" version="1">MTTSIDSSHRHVHKTIPVDDRNSDDSLSDSSIDSMTADLDQIGKLNLPNITSISSWGESLIQMPNFEFSRIQDFSKEFSKYVFNYASSYRRDAGESSQGKEEDDETMLDSIKTVLSTRIQMNKFLNDLKLGVNITTAIDRLQPLTDMIHETIFLPAEDESEYDEEPLDEEEIEPVGVGEIVHKSQPLISEIVKPEMSTRTGSSMASFRKRKRKLFRDLSEDSGEGTSVTSDVDGLTVEEVKELTELNSINDFGEEGVLRNKIQRIQKLDNIDEKSKNKLVTKLMMGNYYKYVNEKLSQENKQLLRPLRKQTLVINGGDGLDNLAEEDEEEQEEAQEDEDMEDEGEDEDEDEDNVVLTAEDTVPSYNDPPFNTTLGCPHYHRNCKVECPTCLKWYPCRFCHDNEITDHKLIRNEVKHILCMHCQTPQVPDTNYCINCEQELANYFCRKCVLYDNDPNKDIYHCDKCGICRLGLGIGKDYFHCDTCNVCLSIDLHDKHKCVTNTTHCNCTICNEYLFTSVQKVVFMKCGHSIHQHCYDELITHSYKCPICKKTIVNVETQFRLLDQEVMQSPLPPPYNAWRCIISCNDCKGKSNCSYHVLGLKCKYCKSYNTNQLKLIKPEEEEEEEEEEIIDGDVMHDFDAKVMRDVRINLSKNFRIDTQVVEDENSGYEDDEEDEEFEDEDTRQEDEYDEVIPENLVDLKKLTNAILSDDYHRINSNVSYITNMFQNFINNATKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATE4</accession>
    <name>G3ATE4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphopyruvate hydratase</fullName>
            <ecNumber evidence="3">4.2.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ENO1</name>
        <name evidence="13" type="ORF">SPAPADRAFT_141919</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(2R)-2-phosphoglycerate = phosphoenolpyruvate + H2O</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Mg(2+) is required for catalysis and for stabilizing the dimer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="3"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376940.1">
        <property type="nucleotide sequence ID" value="XM_007376878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATE4"/>
    <dbReference type="FunCoup" id="G3ATE4">
        <property type="interactions" value="1200"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATE4"/>
    <dbReference type="GeneID" id="18870408"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141919"/>
    <dbReference type="eggNOG" id="KOG2670">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031223_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATE4"/>
    <dbReference type="OMA" id="RCMMSHR"/>
    <dbReference type="OrthoDB" id="1739814at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.10:FF:000001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.120:FF:000002">
        <property type="entry name" value="Enolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF1">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="12">
        <location>
            <begin position="3"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="11">
        <location>
            <begin position="145"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="349"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="376"/>
            <end position="379"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW30907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="440" mass="47045" checksum="DBF9A032B1209695" modified="2011-11-16" version="1">MAITKVFARYVYDSRGNPTVEVDLHTEKGLFRSIVPSGASTGIHEALELRDGDKSKWLGKGVLKAVANVNDIIAPAVVKANLDVTNQAKVDEFLLSLDGTPNKSKLGANAILGVSLAAAKAGAAEVGVPLYKHLAKLANAKDDGKFVLPVPFQNVLNGGSHAGGALAFQEFMIVPTGAETFSEGLRIGSEVYHNLKTLTKKKYGQSAGNVGDEGGVAPDIGSPKEALDLITDAIEKAGYTGRVGIALDVASSEFFKDGKYDLDFKNPESDESKWLSGEQLAALYEELIAAYPIVSIEDPFAEDDWDAWVHFYSKVGSKIQIVGDDLTVTNPIRIKTAIEKKAANNLLLKVNQIGTLTESIQAANDSYAAGWGVMVSHRSGETEDTFIADLSVGLRTGQIKTGAPARSERLAKLNQILRIEEELGDKAIYAGKDFHTAQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATK0</accession>
    <name>G3ATK0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Agglutinin-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_156463</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376996.1">
        <property type="nucleotide sequence ID" value="XM_007376934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATK0"/>
    <dbReference type="FunCoup" id="G3ATK0">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATK0"/>
    <dbReference type="GeneID" id="18871051"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_156463"/>
    <dbReference type="eggNOG" id="ENOG502RGCG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031316_2_0_1"/>
    <dbReference type="InParanoid" id="G3ATK0"/>
    <dbReference type="OMA" id="IHARISN"/>
    <dbReference type="OrthoDB" id="3981162at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903561">
        <property type="term" value="C:extracellular vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030446">
        <property type="term" value="C:hyphal cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030445">
        <property type="term" value="C:yeast-form cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044403">
        <property type="term" value="P:biological process involved in symbiotic interaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030448">
        <property type="term" value="P:hyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2430">
        <property type="entry name" value="Agglutinin-like protein, N-terminal domain, N2 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008966">
        <property type="entry name" value="Adhesion_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024672">
        <property type="entry name" value="Agglutinin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043063">
        <property type="entry name" value="Agglutinin-like_N_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033504">
        <property type="entry name" value="ALS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011252">
        <property type="entry name" value="Fibrogen-bd_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793:SF2">
        <property type="entry name" value="AGGLUTININ-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793">
        <property type="entry name" value="ALPHA-AGGLUTININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11766">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01056">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49401">
        <property type="entry name" value="Bacterial adhesins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442353" description="Agglutinin-like protein N-terminal domain-containing protein" evidence="13">
        <location>
            <begin position="35"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Agglutinin-like protein N-terminal" evidence="14">
        <location>
            <begin position="70"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW30963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="451" mass="49458" checksum="D5AC01B088910738" modified="2011-11-16" version="1">MNLFLFDAPTWSNNQPKMILSLLLLIFFSTIVSSKDNTGFFTSFDSLVWEKGGNYPIISPATPNWIGTFSWTINGTTMEPGDTFTLHMPCVFKFTTHETMIEVGTDDKIFAICTFHPGDIVVAFSELKCTLLDTVTPLTEAYGTLRFPFTFNPGTSAINTDLKCATKFFDGLNTIIFTEGDKELSTQATFEGGYTYTDPDDLVYSNRIIPTLNKMQPYLLGGNCPDGYESGKIGMEILRGTGLIDCNSIHARISNSFNPWYHPRSASNSFDYTTECTSTSFVVEYQNIPAGYRPFIDMLVKVQKNVQLSLKYINTFTCANSSSEVNKGTELTWTPYRNDNADAEGKEVVAITSTYSGRTTSITTLPHQSSDETITIVVAIPVTTITLTSTYDGTTTSYTTLPTDPGNTVSIAFQSPSVAPPQAISVPTAAASISTISTLIVFLYSLTNLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATR3</accession>
    <name>G3ATR3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_73056</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377260.1">
        <property type="nucleotide sequence ID" value="XM_007377198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATR3"/>
    <dbReference type="FunCoup" id="G3ATR3">
        <property type="interactions" value="161"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATR3"/>
    <dbReference type="GeneID" id="18875465"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_73056"/>
    <dbReference type="eggNOG" id="KOG4409">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017361_3_1_1"/>
    <dbReference type="InParanoid" id="G3ATR3"/>
    <dbReference type="OMA" id="AFHSMMQ"/>
    <dbReference type="OrthoDB" id="7457040at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035965">
        <property type="term" value="P:cardiolipin acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF29">
        <property type="entry name" value="PUMMELIG, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="116"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="398" mass="45167" checksum="9B42262C18412386" modified="2011-11-16" version="1">MQSLGISGYDASILLRVSPDGHTAHAYKRSTHPTSVPLSKLFTNSFPLSVRESLEDYGYRHNPLKFQHDLLSTLPFYPHPDSSGTAAKVLQTPIDEHGNFINEFVISPQGNFQEPNHLIMIHGYGGGLGFYLKNFEALAKEKNWCIHAIDLLGYGCSSRPKFVGKNLQEVENWFHDSYSEWLRLRGLDQARDKTLIMAHSMGAYLMGTYGINRDPDFCKKLLMVSPGAIIKHRKQVFVPKYFAKLWEQNISPFSLVRGSGPFGSKLVSMWSSRRFAKLSSNESRLLHKYAYGIFNSPGSGEYMLNFLLAPGADARHPLVERGVHKLKCKLSWWYGKEDWMDPKGGELCSNIINNLRGEVVSDVKLIEDSGHHIYLDNITRFNSMLVNEMRDMEKDEAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATW8</accession>
    <name>G3ATW8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_52437</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377316.1">
        <property type="nucleotide sequence ID" value="XM_007377254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATW8"/>
    <dbReference type="GeneID" id="18871681"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52437"/>
    <dbReference type="eggNOG" id="ENOG502SXTV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111194_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATW8"/>
    <dbReference type="OMA" id="VPKSEYI"/>
    <dbReference type="OrthoDB" id="5632at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="149" mass="17022" checksum="FFA05C124953F3BE" modified="2011-11-16" version="1">MPPKKSTVNLITVLKFKRSRITYTIPFTSTTSLQHLKQQVADAINNSGGVQSDEPEREPVDEEDDEENIPVPKSEFIENGEEEQAEVLVTPEQLRIAVPQDMSSPYDNQWLELDDSMLQKIKFKDYNILAFGVDDEPFDIVEAAYEEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATY8</accession>
    <name>G3ATY8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63213</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377335.1">
        <property type="nucleotide sequence ID" value="XM_007377273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATY8"/>
    <dbReference type="GeneID" id="18874645"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63213"/>
    <dbReference type="eggNOG" id="ENOG502T647">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2293458_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATY8"/>
    <dbReference type="OMA" id="NLLFRAY"/>
    <dbReference type="OrthoDB" id="4018249at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="101" mass="11497" checksum="714F9D9532EC3020" modified="2011-11-16" version="1">MTQMKTSIIPHKLKRLYDLYYYCSPIPVLSTGETIIFNLINLTILLVSAYYAVYFLPTVLTRAIEKLWFYITGQIVIVNSFFPFALSNSSQIVGASKNFTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUC8</accession>
    <name>G3AUC8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ornithine carbamoyltransferase</fullName>
            <ecNumber evidence="3">2.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Ornithine transcarbamylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">OTC/ARG3</name>
        <name evidence="11" type="ORF">SPAPADRAFT_63324</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="3"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377475.1">
        <property type="nucleotide sequence ID" value="XM_007377413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUC8"/>
    <dbReference type="FunCoup" id="G3AUC8">
        <property type="interactions" value="674"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUC8"/>
    <dbReference type="GeneID" id="18874711"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63324"/>
    <dbReference type="eggNOG" id="KOG1504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_3_0_1"/>
    <dbReference type="InParanoid" id="G3AUC8"/>
    <dbReference type="OMA" id="DGNNVCN"/>
    <dbReference type="OrthoDB" id="10252326at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903269">
        <property type="term" value="C:ornithine carbamoyltransferase inhibitor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019240">
        <property type="term" value="P:citrulline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000017">
        <property type="entry name" value="Ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000009">
        <property type="entry name" value="Ornithine carbamoyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001986">
        <property type="entry name" value="PRK00779.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753">
        <property type="entry name" value="ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753:SF3">
        <property type="entry name" value="ORNITHINE TRANSCARBAMYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase carbamoyl-P binding" evidence="10">
        <location>
            <begin position="17"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase Asp/Orn-binding" evidence="9">
        <location>
            <begin position="167"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="331" mass="36914" checksum="F43FF010FC480940" modified="2011-11-16" version="1">MAVRSVTSKISSTLKPRHLVSISQLTNDEFSSLVNKAQHFKQIVKSGETSVENHQKLLGKLVALLFSKRSTRTRISTEGAAAFFGAQPMFLGKDDIQLGVNESMYDTTRVISSMTSCIFARVNRHQDILDLCKDSSVPIINSLCDKYHPLQAIADLLTIKEVFGETKGLKLTWIGDANNVINDLAIACLKLGINVSVSIPKDIKFDTDVQQVAEELAKEQDLSFEIVNDPIVALKNANVIVTDTWISMGEEEQKQQKLQQFAGYQITKSMIEKGQAASNWQFMHCLPRHHEEVDDEVFYSDKSVVFQEAENRLYAAMAVIDGFVINKGKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUN8</accession>
    <name>G3AUN8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SCF-associated factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_157841</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377565.1">
        <property type="nucleotide sequence ID" value="XM_007377503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUN8"/>
    <dbReference type="FunCoup" id="G3AUN8">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUN8"/>
    <dbReference type="GeneID" id="18871102"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157841"/>
    <dbReference type="eggNOG" id="ENOG502QUVE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_460835_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUN8"/>
    <dbReference type="OMA" id="MGDYHYL"/>
    <dbReference type="OrthoDB" id="61110at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF3">
        <property type="entry name" value="F-BOX PROTEIN POF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="87"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="168"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="617" mass="69322" checksum="07BFFC4D66852723" modified="2011-11-16" version="1">MALTILDLGEDILTSEIPKYLSPEDIFNLSLVNKYIYNLYHCSILSTRLYQILYNKKFTNNENNYTISLKNKLNWKQLFQLRCSRDQKVYTWGEPQSGRLGFLPSRVDHSHVSFGGWAVHTPTNISEFNDHLIIDIVANGFSFIILTNAGELWFTGITWKGMANRQQLSTPGPISNPDYRPSPGELALDTLHSQTGNGNVASRRSIRGVMPIPLMNRRYRDDDADDSGSNTGSNSPTPPPGPTNVGLPQSRRRLQPQPVETGEPDLSSIPDLNHPPSKIQETNFLTRLYLPPIADIQQQKTRKIVSISTGREHIIALDNHNNVYSWDTGCSTNMGVHVTFPGIDKGALVTKIVAGWNLSACTIAGYGLVIWYSRMPLSKSQYDQSIFESQARYFIVPFTKDDIVDFTVGSDYVLYIKQSEDKLYQFRMDAHDFAQRNVDSVISNDELFNCISPMDNFNNWKLNYENQGMENIKFTKLNSCYTNFVVFTNHDQVLVGSRKHLVYENEDGDEKGAKPKIIPELQGQNIKKLVIGDYHYLALTNDGDILSWGRESGRCGCLGLGDTESVANEHPESARVDGTALEVTKPLRVKNPPYPGKWVALTASGWHSGGIYVPVDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUW4</accession>
    <name>G3AUW4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_52892</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377821.1">
        <property type="nucleotide sequence ID" value="XM_007377759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUW4"/>
    <dbReference type="STRING" id="619300.G3AUW4"/>
    <dbReference type="GeneID" id="18871731"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52892"/>
    <dbReference type="eggNOG" id="ENOG502RY38">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_509169_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUW4"/>
    <dbReference type="OMA" id="EYWIYEN"/>
    <dbReference type="OrthoDB" id="2240312at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000500">
        <property type="term" value="C:RNA polymerase I upstream activating factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000182">
        <property type="term" value="F:rDNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001181">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039601">
        <property type="entry name" value="Rrn5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28079">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28079:SF1">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="457"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="413"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="61536" checksum="785B739544DF3D33" modified="2011-11-16" version="1">MSAEEDTPAPTSPPAPTSIPPGPAPPVISRSEGLRPSRVFIGNRAYVDFYNRFVEETFTYSNSYTTSHLRDSIIAHNGQVYYRSEEGTITEHETPGTFWSWQEKERFFDALSKCSIHRADLIQERVKTKSILEIMNYYTHLKKSTEALKNRKSTVVNRFGLKRNWSTKLLTYHEMPIAYEISPYFINFEELQTESIGYRERTFELAKATKLKSSFAIYEDLSLINLDNFKKLFSLNLGFPALILIEELIRLLTKRIILKVISTHSNSIRKFDIDQAIASLNYNDPDSLFNYRNMWIEVSNLQKEKRNLYEPTLYLDPTFTIPQKHQGENPKGIMHINSLRKGNIANISEELDEELEDMLIEHETEELEALDQMNSKVHERGLATMLLPSAYYEPEKAQDLAALWLTEQRELENESESESESEEEDVDNTSELNQQVAPEQIQSNVAEADTQISLELIDPELTNRTPSVAPEDVPIEVQYTCQEPSTQEPPPAELPNQEPAAEEPPTKKRKISPSTIHRLVVDSYTYTYANYDKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV46</accession>
    <name>G3AV46_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dihydrofolate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_63736</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377886.1">
        <property type="nucleotide sequence ID" value="XM_007377824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV46"/>
    <dbReference type="FunCoup" id="G3AV46">
        <property type="interactions" value="194"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV46"/>
    <dbReference type="GeneID" id="18874889"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63736"/>
    <dbReference type="eggNOG" id="KOG2525">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015869_2_0_1"/>
    <dbReference type="InParanoid" id="G3AV46"/>
    <dbReference type="OMA" id="RERFCIN"/>
    <dbReference type="OrthoDB" id="5212574at2759"/>
    <dbReference type="UniPathway" id="UPA00850"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008841">
        <property type="term" value="F:dihydrofolate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.10:FF:000010">
        <property type="entry name" value="Dihydrofolate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF0">
        <property type="entry name" value="DIHYDROFOLATE SYNTHETASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01011">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01012">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="39658" checksum="1EB19EDCC97B3DFC" modified="2011-11-16" version="1" fragment="single">MPIDLGLTRVTKLLAHLANPHLSYKSIHIAGTNGKGSVLAYISSILTTAQIRNGKFTSPHLIEYHDCITINNQVYPKSKFEQVQQLVGMTNEQHKLNCTEFELLTATAFKIFEIENVDIALIEVGVGGLTDATNVLEPYNGKTGVIACGITKIGLDHQGLLGGTLHEIAKVKSGIMKPGIKCFVDGSNEPSVLDEIRATAKEVANEVEIVQPELELIQYSPLHGQYQANNLAISLGIIKTVSHYYPQSISQEIIIQGIKSTKWPGRLQSVVHPTIGEILVDGAHNESAAIELGKYINREYDPSKKGIIYVIGITKGKSASDLLKHVVRSIDTVVFTTFTQPENMPWVKSTPVEELTEITQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVA3</accession>
    <name>G3AVA3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Alpha-1,2-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_158071</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377672.1">
        <property type="nucleotide sequence ID" value="XM_007377610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVA3"/>
    <dbReference type="STRING" id="619300.G3AVA3"/>
    <dbReference type="GeneID" id="18871112"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_158071"/>
    <dbReference type="eggNOG" id="ENOG502QVWG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013298_1_0_1"/>
    <dbReference type="InParanoid" id="G3AVA3"/>
    <dbReference type="OMA" id="HVMNERY"/>
    <dbReference type="OrthoDB" id="4484309at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646:SF1">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW29906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="710" mass="81420" checksum="BDBE9262EA741840" modified="2011-11-16" version="1">MAYLGSQQSISFLLLCGLTAVIVIFNVIALTPSKENTIEYNSSGGTYYVGSKKLQNEVNKVDSEAFMTDLFKELQETKKDELIQLMKKEMEAELKPIIVSELKAEYRSTHLEDVKKALKEQVLNEFTDEFYLEKRVSYSIFEDLKADYLRKNGRNIQNYAVLQALKDEGLPEIQLPLGFEIKLKNRMERYVDRSKYFSHLLDDILLKAKPECDALTSSERGSNLDPTYQWDSRLLSESILRGTELNIPGPKFEALQKAHDQVVQRLKFLPDPLPEIIQGDGIVINGVRESMRSILITVASLKDSGSTLPIEVILETNQDYDSDVCDKLLPTLNGKCVILEKEVKLSQSNLLSDHHTRKILGILVSSFDNVIVIDAGNLVLKNIDSIFHSDPFLSAKMILWPDRWHKMTSPVYYRLARIHPGEMIRRDGIANDQPIVRILPKNKGSSVHFHDLENIPKSVSVDSGQMVVSKKEHWKSLLLALYYNMHLKDFYQPMFYQGAPGSERETIVPALHVMNERYFLEAQKFPTYNIDNHETAFAQLDPRQVATFYNDWKEFLAERDMDTRLNPYQAGFYTETLVKEFIASKKKLMKEVQVDIEPALYRMSEYTPPESLFLHPHNPRIDAVENSKQSNTYKRRNLGPAPAAGSIDWELKFNTMAKWVVCNAITSDSYWTKVSADQKEVCNSVSQYVEFLKKDTRDPKSGELSFAKMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVF4</accession>
    <name>G3AVF4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Arginine biosynthesis bifunctional protein ArgJ, mitochondrial</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="10">Glutamate N-acetyltransferase</fullName>
                <shortName evidence="10">GAT</shortName>
                <ecNumber evidence="10">2.3.1.35</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">Ornithine acetyltransferase</fullName>
                <shortName evidence="10">OATase</shortName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="10">Ornithine transacetylase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="10">Amino-acid acetyltransferase</fullName>
                <ecNumber evidence="10">2.3.1.1</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">N-acetylglutamate synthase</fullName>
                <shortName evidence="10">AGS</shortName>
            </alternativeName>
        </domain>
        <component>
            <recommendedName>
                <fullName evidence="10">Arginine biosynthesis bifunctional protein ArgJ alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="10">Arginine biosynthesis bifunctional protein ArgJ beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_63788</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-glutamate + acetyl-CoA = N-acetyl-L-glutamate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(2)-acetyl-L-ornithine + L-glutamate = N-acetyl-L-glutamate + L-ornithine</text>
            <dbReference type="Rhea" id="RHEA:15349"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.3.1.35"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="10">The alpha and beta chains are autoproteolytically processed from a single precursor protein within the mitochondrion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ArgJ family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.35" evidence="10"/>
    <dbReference type="EC" id="2.3.1.1" evidence="10"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377939.1">
        <property type="nucleotide sequence ID" value="XM_007377877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVF4"/>
    <dbReference type="FunCoup" id="G3AVF4">
        <property type="interactions" value="296"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVF4"/>
    <dbReference type="MEROPS" id="T05.001"/>
    <dbReference type="GeneID" id="18874913"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63788"/>
    <dbReference type="eggNOG" id="KOG2786">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027172_1_0_1"/>
    <dbReference type="InParanoid" id="G3AVF4"/>
    <dbReference type="OMA" id="WGRIVMA"/>
    <dbReference type="OrthoDB" id="2017946at2759"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00106"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004042">
        <property type="term" value="F:L-glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004358">
        <property type="term" value="F:L-glutamate N-acetyltransferase activity, acting on acetyl-L-ornithine as donor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006592">
        <property type="term" value="P:ornithine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02152">
        <property type="entry name" value="OAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.70.12:FF:000001">
        <property type="entry name" value="Arginine biosynthesis bifunctional protein ArgJ, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.340:FF:000002">
        <property type="entry name" value="Arginine biosynthesis bifunctional protein ArgJ, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2330.10:FF:000001">
        <property type="entry name" value="Arginine biosynthesis bifunctional protein ArgJ, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2330.10">
        <property type="entry name" value="arginine biosynthesis bifunctional protein suprefamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.340">
        <property type="entry name" value="ArgJ beta chain, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.70.12">
        <property type="entry name" value="L-amino peptidase D-ALA esterase/amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01106">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002813">
        <property type="entry name" value="Arg_biosynth_ArgJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042195">
        <property type="entry name" value="ArgJ_beta_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00120">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003802">
        <property type="entry name" value="PRK05388.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100">
        <property type="entry name" value="ARGININE BIOSYNTHESIS BIFUNCTIONAL PROTEIN ARGJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100:SF0">
        <property type="entry name" value="ARGININE BIOSYNTHESIS BIFUNCTIONAL PROTEIN ARGJ, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01960">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="DmpA/ArgJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 10" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 10" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_5023451848" description="Arginine biosynthesis bifunctional protein ArgJ alpha chain" evidence="10">
        <location>
            <begin position="1"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023451847" description="Arginine biosynthesis bifunctional protein ArgJ beta chain" evidence="10">
        <location>
            <begin position="211"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="430"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="435"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="10">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="10">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="10">
        <location>
            <begin position="210"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="435" mass="46330" checksum="9D30A5FA9F71FD81" modified="2011-11-16" version="1">MHPVTKVSIRHLSKAARFVPKAGVYPQGFKVGGIHCGVKKDGHSFDLALLQNTFGKEATAAAVFTTNKFKAAPVQVSSKILKEKHGSGINSLVINSGNANAVTGAQGIKDAEDMVIVTDSVCENPKNSTLVMSTGVIGNNLPIDKILTGIPKLALSNLGDSHEHWINCATAICTTDTFPKLVSKQFTIGEDTYTLAGLCKGAGMICPNMATLLGFFVTDAPVSASALQQILKYSVDRSFNSITVDGDMSTNDTIVAIANGAAGGELIDNNSSCAERYARLQAEITSFAQQLAQLVVRDGEGATKFITIRVKDAVSYLEAKNIASSVANSSLFKTAMYGKDANWGRILCAIGYANAQNVIPEKTSVKFIPTDGSEHLELLVNGEPEVVDEARASQILELEDLEIEINLGTGAGQEATFWTCDLSHEYITINGDYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVI7</accession>
    <name>G3AVI7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Coronin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_63558</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Binds to F-actin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the WD repeat coronin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377702.1">
        <property type="nucleotide sequence ID" value="XM_007377640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVI7"/>
    <dbReference type="FunCoup" id="G3AVI7">
        <property type="interactions" value="363"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVI7"/>
    <dbReference type="GeneID" id="18874805"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63558"/>
    <dbReference type="eggNOG" id="KOG0303">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026859_3_2_1"/>
    <dbReference type="InParanoid" id="G3AVI7"/>
    <dbReference type="OMA" id="NFQDDIY"/>
    <dbReference type="OrthoDB" id="1850764at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990819">
        <property type="term" value="C:mating projection actin fusion focus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110085">
        <property type="term" value="C:mitotic actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071846">
        <property type="term" value="P:actin filament debranching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034316">
        <property type="term" value="P:negative regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000601">
        <property type="term" value="P:positive regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="Coronin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015505">
        <property type="entry name" value="Coronin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015048">
        <property type="entry name" value="DUF1899"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
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        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856:SF0">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08953">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16300">
        <property type="entry name" value="WD40_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM01166">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01167">
        <property type="entry name" value="DUF1900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="DUF1899" evidence="12">
        <location>
            <begin position="4"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="77"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="137"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="179"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="412"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="596"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="412"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="473"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="495"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU280818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW29936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="637" mass="71318" checksum="F623ADCAF59EE00E" modified="2011-11-16" version="1">MSGKFVRASKYRHVFGQAAKREACYENLKITKNAWDSNIIHTNGKYLSVNWDISGGGAFAIIPLNEVGKAPDTVPLFRGHKGPVLDTAFNPFDKQQIVSCSDDGKILVWNIPEDYSFHKYVDADDNIKDITEPVKVLSGHTRKVGLVEFHPCAKNILASSSLDYTVKIWNLETGKDVITLEHKDLVASFSFNYNGSLLATTSRDKKIRVWDIRSGKIISEGPGHNGAKPSRVCWLGNTDRIVTTGFSRLSDRQVGIWDINNIDKGPIDGFFNVDASSGVLIPSFDPDTSILYIAGKGDGNIRYYEYENDNLFELSQYASTDPQRGFAMIPKHSVNVKENEIGRVFKTVLDNSIEPVSFIVPRRSELFQADIYPDCPSEQPALTAEEWLSGKEVNGPLLMSMEALYEGTEPEIHESQPGVKVDEAKEEAEQKKKEEEEQQQQQAKELSPKPATPQVKEDSGRSTPAQNVDELLKSSSEVNSLLNKVVDQSDDEDAAEYHEDKDESWEEVPKQQEIAKEIPREEPKEVKEEPKKEEPKKEEPKKEEPKVEAKEQVKEEPPKEQPKEVKEEPKAEKKEATPAREASATPSTKPTLASTIEKLANLVVSLESQVTKLTQAGLEKDERLASLEAKIEQLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVQ0</accession>
    <name>G3AVQ0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ribosome biogenesis protein ALB1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_144296</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377981.1">
        <property type="nucleotide sequence ID" value="XM_007377919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVQ0"/>
    <dbReference type="FunCoup" id="G3AVQ0">
        <property type="interactions" value="236"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVQ0"/>
    <dbReference type="GeneID" id="18870612"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144296"/>
    <dbReference type="eggNOG" id="ENOG502S14D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103824_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVQ0"/>
    <dbReference type="OMA" id="HHKVHSL"/>
    <dbReference type="OrthoDB" id="4086742at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022784">
        <property type="entry name" value="Ribosome_bgen_Alb1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09135">
        <property type="entry name" value="Alb1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="156" mass="16976" checksum="14D8124D4EE5B433" modified="2011-11-16" version="1">MAGKNNINKPKIKLNANSHAKHLGSKRAARARSNASTRSSLGRYADKATTAPRPTDSVALALYTGETPGPVNTMTNVTLSKKRAKKIARNQKYFAANIKTKSDDVTMEIDNETVEEKQSKLDQIKKALWSVIENHSKNGYSVVADAEGTTLGVQSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADX6</accession>
    <name>G3ADX6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_57764</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372112.1">
        <property type="nucleotide sequence ID" value="XM_007372050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ADX6"/>
    <dbReference type="GeneID" id="18872185"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57764"/>
    <dbReference type="eggNOG" id="ENOG502RPZ5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_921408_0_0_1"/>
    <dbReference type="InParanoid" id="G3ADX6"/>
    <dbReference type="OMA" id="VKYCHSA"/>
    <dbReference type="OrthoDB" id="4083114at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="61"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="289" mass="33655" checksum="D52B773713246196" modified="2011-11-16" version="1">MNHSMPGSFPGSYGSTSYQPRQLQKATTNERLASQLTQSTEMTRFFNTTYINTKTKAQHHLIGQLGLIVYVLAFYQYVKYCHSAALIPLLFHCIYQVVLSIEILTNANLQVIRHLLSDTTEPEEQQFVLNNRMRFVCRMLYTKCLFVLLYHILFVCSWMVSIVNRGQLQELTYGTWWVVSFIGEEVPDISPEVPYHEKLLKLGLIQLLFIDLIVLFLELVMFQCIYKQSPILKIERRLNEQEIYCVRQSTSPNISVNEEDVSQEDVPTVLKVKLYQCFKREGYLDVIGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE63</accession>
    <name>G3AE63_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein-lysine N-methyltransferase EFM5</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Elongation factor methyltransferase 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">EFM5</name>
        <name evidence="6" type="ORF">SPAPADRAFT_58814</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that trimethylates elongation factor 1-alpha at 'Lys-79'.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. EFM5 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373009.1">
        <property type="nucleotide sequence ID" value="XM_007372947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE63"/>
    <dbReference type="FunCoup" id="G3AE63">
        <property type="interactions" value="302"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AE63"/>
    <dbReference type="GeneID" id="18872623"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58814"/>
    <dbReference type="eggNOG" id="KOG3350">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074410_1_0_1"/>
    <dbReference type="InParanoid" id="G3AE63"/>
    <dbReference type="OMA" id="CNFRPEH"/>
    <dbReference type="OrthoDB" id="206354at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03187">
        <property type="entry name" value="Methyltr_EFM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019369">
        <property type="entry name" value="Efm5/EEF1AKMT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041370">
        <property type="entry name" value="Mlase_EEF1AKMT1/ZCCHC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13200">
        <property type="entry name" value="EEF1A LYSINE METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13200:SF0">
        <property type="entry name" value="EEF1A LYSINE METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10237">
        <property type="entry name" value="N6-adenineMlase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="250" mass="28761" checksum="3298588055614F8A" modified="2011-11-16" version="1">MSDSDDEPLVLSAHALAALTEFKREEKDRLQQFESLYKASEDKFEEQKAVSIDAFKEDWQLSQFWYTDETAKTLGRALLEGADEDTVIAIASAPSVYAAIKQLPKEEIPTKHIYLLEYDRRFEVLAGIDHFSVYDYNSPDLIPSHLRNKCHRLLIDPPFLEPECQTKSAQAAKNLLVTNKDDRTRSGDSKYKLISSTGERMQSIVKKNYPDTHMTTFLPEHKNGLSNEFRCYASFECKYWKFQKDPSTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEJ8</accession>
    <name>G3AEJ8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Septum-promoting GTP-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_145190</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372172.1">
        <property type="nucleotide sequence ID" value="XM_007372110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEJ8"/>
    <dbReference type="FunCoup" id="G3AEJ8">
        <property type="interactions" value="571"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEJ8"/>
    <dbReference type="GeneID" id="18870674"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_145190"/>
    <dbReference type="eggNOG" id="KOG1673">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_0_2_1"/>
    <dbReference type="InParanoid" id="G3AEJ8"/>
    <dbReference type="OMA" id="FDLNCTI"/>
    <dbReference type="OrthoDB" id="6585768at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040001">
        <property type="term" value="P:establishment of mitotic spindle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904750">
        <property type="term" value="P:negative regulation of protein localization to nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031536">
        <property type="term" value="P:positive regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023056">
        <property type="term" value="P:positive regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902542">
        <property type="term" value="P:regulation of protein localization to mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04128">
        <property type="entry name" value="Spg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000330">
        <property type="entry name" value="Septum-promoting GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017231">
        <property type="entry name" value="Small_GTPase_Tem1/Spg1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037527">
        <property type="entry name" value="Small_GTPase_Tem1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="22963" checksum="1B7750C909347962" modified="2011-11-16" version="1">MPDIEDDNQDQNNQVALKIGLIGDSQIGKTSLMVKYVEGSFDEDYIQTLGVNFMDKKIQIRNTTITFSIWDLGGQKEFINMLPLVSNDAVAILFMFDLTRKSTLNSIKEWYRQARGFNKTAIPFLVGTKYDQFIDLSFQDQLEITQQAKKFGSAMKAPVVFCSTSHSINVQKIFKIILSKAFDLKLNLDEITNVGEPILIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEP1</accession>
    <name>G3AEP1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mediator complex subunit 12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_133266</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 12 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373079.1">
        <property type="nucleotide sequence ID" value="XM_007373017.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AEP1">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEP1"/>
    <dbReference type="GeneID" id="18869734"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133266"/>
    <dbReference type="eggNOG" id="KOG4522">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003142_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEP1"/>
    <dbReference type="OMA" id="HWLQEWT"/>
    <dbReference type="OrthoDB" id="20828at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019035">
        <property type="entry name" value="Mediator_Med12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46567">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46567:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09497">
        <property type="entry name" value="Med12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01281">
        <property type="entry name" value="Med12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med12" evidence="9">
        <location>
            <begin position="162"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="40"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1409"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1559" mass="179566" checksum="C3DD31A5D2374885" modified="2011-11-16" version="1">MSKPRSRNSLLSSHNRTSYSNSTKDELLAMKYQMDKPALPLYPLSDLNTNSSSDTDSSKRDHEEHETTYPDFKPWKDHTQLAGDKQEQEIQKMSNTSFLNKGYFEAPQVSNEYYSARNLIQATVFSSTDNCNYVLRELSQHLANAYKTRNEVINKIKYDSNNFRIPPRVTLTATKKEAWLKDLGNSNIPIMKIGEKIPHGIRNKVLIDSVCSRNVPLNRALWFTKCVLFSELLALRRKHQARLSVSGPIPGSEFNTVEKFEIHWLQEWTQQVCDYILKFSREMVHITTPEKKEHYMNKLTYLLTYVQSLYVECLVDKTFFLSVIFKSLKDGLPLEVKHINELVFTSNDDEKLTETWIDEIDLNYGQRLVSLTLIKIFWKDILKTDYLCKELSELLLLNHLFINRISSLESHSYHHKFALPETLAQNVLEMISNTVIYLFKYNTNVFIIPKYWILLQDPLVKIMVEHGESQLENEQDEIKNQLELIKYRNESLILNLKHVAEHKQPKVRARSNSFSNMTPIENDINTFMNRDTNDILRILCCLDRVKLNNDLASLLKPTTHGIRKTGCPNWRINLRLVLFWCITPYRSHKNSSEGILIVCNFLKKHILQSLHGSIRTEFETELLEIIYQIAESYESLFVKYNLYVLINELYQLKVLTIASYLRKLIASGIFFLAPGTTEDLSNLPPVVKIHLDILQSLPVLNNRQCDSILKKWTPEGFNFKDKFDRGQQILQTEIIDRLVNNTFNVNIESNWNYIKSLNVGLKFLLVNWITNVLKTTLNDSPKLIHFDPVVIANLYMFYSNCDNLTVFFKVLVKTILRNEGGMIIFYLDSLYFIAKLVIHHFKLIKSLAGSNDLSSTGYELFKLIVTTYKDLSQREYNYFSFNQVWNFIDDVIEKEYHEKAKRSKVLENQLFSKDTMDTPMNINTVDVHSKSSSSSDRYTSADFRNDLETLRNSRVQALDPSEVQDTLDTLNLEISELSLSEAVTAQLEYLFKQGISQDQENLIIKLLFNARISLKEDGSFNELVRQFVMNVMQSEADFNIKANFLKKLVISEIFKMNDLVSFCQTESQAGPLVMDVIIGNEGEAANLAPNESLGLSIHRVTFREKSMANFISILSKELLKGSIFHSDLMAKHKESMLKYIHDALIIHPKLLFEELFVKLSKEDCIDLLNLLLGREHTTFIRQLSDFEILTPDINEFNLPIVQMLIKIISYYELNNSDDIRQKLELLLGTLISKCEFKFSASNSFFGELFSFISWEHKIFILDIIETEFLTNTKFEETISLDNGRDLLPPFSDFFNKFSLSSANTVDCSPVFFTNLCEFLTKLTKAVNTPHLFTDSLSTSILIFLRILIIHNSALCKLISIGQETHVLFIQNLLAILHSQFLGSHEKLRILLYDLLLLMKSSVTQELTNAMEPDHEGTSPGFSSSAPSDDSKKNGSVPIVPSMSIQRVFELPDEPKDDNPFKDNIDESLVQCALMLDDEELNHGGDVHAFNDSGLVLLQARPHSVSLPFGILNNNEKVSNEIKMVKFRSFEILEDTSSALNDGCVNLQLLDAYTTRENPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEP4</accession>
    <name>G3AEP4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="6">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_58878</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="6"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373082.1">
        <property type="nucleotide sequence ID" value="XM_007373020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEP4"/>
    <dbReference type="FunCoup" id="G3AEP4">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEP4"/>
    <dbReference type="GeneID" id="18872659"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58878"/>
    <dbReference type="eggNOG" id="KOG0372">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004962_0_5_1"/>
    <dbReference type="InParanoid" id="G3AEP4"/>
    <dbReference type="OMA" id="KIGGYPP"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901525">
        <property type="term" value="P:negative regulation of mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000168">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="7">
        <location>
            <begin position="119"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="383" mass="43654" checksum="1FEECB9DAA5B3952" modified="2011-11-16" version="1">MTIPYNIPISDLDYCLEQLLDHKPPKILPPETIQHLCHTLKTQLLSVPNIMPLQSPISIVGDIHGQFHDLLEIFQIGGSPPSTNYLFLGDYVDRGYYSVETISLLIALKLRYPDRVFLIRGNHESRTITTNYGFYTEVLNKYNGSAEVWTYITDLFDYLPLGATIDGKIFAAHGGLSPSCQQLDQIRALDRFREIPHDGIMADLVWSDPDTEILDFKLSPRGAGYLFGLDVINKFCHDNDLMQMVRAHQLCNEGYTSYWKGKCLTVWSAPNYCYRCGNRASVLEILYYSGESKSMGEPNANENDVKYDDYKNRFEDSIVHKVQGVLPGQFFNIFEASPENDEDTSKGKGVNGINSDEELVGNGDFFCSYFKERPRKQHVEYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEZ4</accession>
    <name>G3AEZ4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_57870</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372210.1">
        <property type="nucleotide sequence ID" value="XM_007372148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEZ4"/>
    <dbReference type="GeneID" id="18872230"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57870"/>
    <dbReference type="eggNOG" id="ENOG502SECK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100670_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEZ4"/>
    <dbReference type="OMA" id="MAPPKRF"/>
    <dbReference type="OrthoDB" id="4093188at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013175">
        <property type="entry name" value="INO80_su_Ies4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28061">
        <property type="entry name" value="INO EIGHTY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28061:SF1">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08193">
        <property type="entry name" value="INO80_Ies4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="176" mass="19284" checksum="6149AB167F6E996C" modified="2011-11-16" version="1">MAPPKRYWVKLKVPASTLSKLPDFPTPISKSRLKKIAAEERRAGGHSHSLSPSERSSPAPEETASRINPASLNNLVPGGGSNSTSGAISQFKINQGLKESSTSGLTMNSITSGLYALDKSGKPVRKWIKKPREFKTFSGFKVRYVSYQHKDGKKEKKEKETKVEPESVTPEVKSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFM4</accession>
    <name>G3AFM4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Histone H4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_58143</name>
        <name evidence="14" type="ORF">SPAPADRAFT_59417</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907</strain>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the histone H4 family.</text>
    </comment>
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        <property type="protein sequence ID" value="EGW34004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="EGW35013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372425.1">
        <property type="nucleotide sequence ID" value="XM_007372363.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373588.1">
        <property type="nucleotide sequence ID" value="XM_007373526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFM4"/>
    <dbReference type="FunCoup" id="G3AFM4">
        <property type="interactions" value="1341"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFM4"/>
    <dbReference type="GeneID" id="18872339"/>
    <dbReference type="GeneID" id="18872906"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58143"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59417"/>
    <dbReference type="eggNOG" id="KOG3467">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109117_2_3_1"/>
    <dbReference type="OMA" id="QKEHING"/>
    <dbReference type="OrthoDB" id="3972251at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:protein heterodimerization activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:structural constituent of chromatin"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="HFD_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000007">
        <property type="entry name" value="Histone H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CENP-T/H4_C"/>
    </dbReference>
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        <property type="entry name" value="Histone-fold"/>
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    <dbReference type="InterPro" id="IPR001951">
        <property type="entry name" value="Histone_H4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019809">
        <property type="entry name" value="Histone_H4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004823">
        <property type="entry name" value="TAF_TATA-bd_Histone-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10484">
        <property type="entry name" value="HISTONE H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15511">
        <property type="entry name" value="CENP-T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00623">
        <property type="entry name" value="HISTONEH4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00417">
        <property type="entry name" value="H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00803">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00047">
        <property type="entry name" value="HISTONE_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="10 11" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TATA box binding protein associated factor (TAF) histone-like fold" evidence="13">
        <location>
            <begin position="28"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW35013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="103" mass="11462" checksum="17D362C47448D99B" modified="2011-11-16" version="1">MSGRGKGGKGLGKGGAKRHRKILRDNIQGITKPAIRRLARRGGVKRISALIYEEVRVVLKQFLENVIRDAVTYTEHAKRKTVTSLDVVYALKRQGRTLYGFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFT1</accession>
    <name>G3AFT1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_53419</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007372420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFT1"/>
    <dbReference type="FunCoup" id="G3AFT1">
        <property type="interactions" value="92"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFT1"/>
    <dbReference type="GeneID" id="18871783"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53419"/>
    <dbReference type="eggNOG" id="KOG0274">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_408316_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFT1"/>
    <dbReference type="OMA" id="CTTPQGC"/>
    <dbReference type="OrthoDB" id="2095648at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
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    <dbReference type="InterPro" id="IPR052301">
        <property type="entry name" value="SCF_F-box/WD-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14381">
        <property type="entry name" value="DACTYLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14381:SF1">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="6"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="577"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="584"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="668" mass="77025" checksum="97D9752046E6DE63" modified="2011-11-16" version="1">MCKKRGLNINDLPIELLIAIFSELDPVYLNIMRLVCKKWNFVINDRETWTNAFTNKFHTPKIFPSLSSNCMTEYFMRLNVAKNWKKGHATHSFYQLINNEYRFNDLTRCDFKQDRIMIFSKRHGNITSGNLHSGKNQSFIPGGSNLNITSSCMNGNYLLTGKLNGEVHLKNLVTSTSVTSRSSYQVFGEPSDSPIMCVCMNDYPDKFKSKVDAISGGYKGDLKCWNFQGKLVRQFQFEQEVVYNVLSDFQKYIILNTDKRVVIIDYQKLEILKEYDMGFTIVNTQDPNTASYYDHLIHYKNKLDIDYSDKNIILCYDTNIKVFNFETGIERQLHVNVPIVKSYLQTCSESKLLASRNRSIIGGDGLLYVNLLADESVIIWNIRDDSREIEPIVHIQPSLNYMKYSPMIHDAIVQRDLPFVVDVAVTSTVLAVCGYNGLCNIYDVYTGKYLREISLKFPKKFEHMHDRLIGDVYIKLNPNQLDCNGVIVCGDTVQYFQFGDLQPDTVNKPKKQKSVNLGIHSKHESKKKIQEGMDEYHTQQYQQRMKQALLDKYNGTLYDDEDEELSIALAISESYHNSTGDVSQLEEEEGGVGEDYEEQRREQEVEQALDEDLLLAIELSKQEEENKKIVDQIINGPVEMSSSQHAMLNDEEIDEELRRALELSLIEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFW0</accession>
    <name>G3AFW0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">T-complex protein 1 subunit delta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_58242</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372511.1">
        <property type="nucleotide sequence ID" value="XM_007372449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFW0"/>
    <dbReference type="FunCoup" id="G3AFW0">
        <property type="interactions" value="1427"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFW0"/>
    <dbReference type="GeneID" id="18872381"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58242"/>
    <dbReference type="eggNOG" id="KOG0358">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_9_1_1"/>
    <dbReference type="InParanoid" id="G3AFW0"/>
    <dbReference type="OMA" id="HPAANMI"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03338">
        <property type="entry name" value="TCP1_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000010">
        <property type="entry name" value="T-complex protein 1 subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012717">
        <property type="entry name" value="Chap_CCT_delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02342">
        <property type="entry name" value="chap_CCT_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW35099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="533" mass="58273" checksum="EE86A536988C8BA5" modified="2011-11-16" version="1">MAQVARPAVAPSNATFKDKEKPQEVRRANILAARAVSDAIRTSLGPKGMDKMIKTKTGEIIISNDGATILQHMAVLHPAARMLVDVSRAQDVEAGDGTTSVAILTGAFLGAADRLLNKGIHPTLIAESFQRAAQRACEVLLDMSYEVKLDDREQLIRAASTSLSSKIVSQHSQLLAPLSVDSVSKVINEEKNNVDLNDIRLIKKVGGTIDDTKLINGVVLTQNVVKNANGPIRMEKAKIALIQFQLSPPKPDMENNVVVNDYRQMDKILKEERAYLLNICKKIKKSKCNVLLIQKSILRDAVNDLALHFLAKLNIMVIKDIERDEVEFLSKALGCKPIADIDNFTEDRLGSADLVEEIESSGSKIVQITGITSKLTKPTVSVIVRGANNLVLDETERSIHDALCVIRCLVKQKALIAGGGAPEIEVSRTLMRESNKLSGVEQFVYQEFAQALEVIPTTLAENAGLNPINVVTDLRNRHENGEKDAGISVRRSGTSNTYDEHILQPVLVSTSAISLASECVKSILRIDDIQFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFW8</accession>
    <name>G3AFW8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_131868</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372519.1">
        <property type="nucleotide sequence ID" value="XM_007372457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFW8"/>
    <dbReference type="GeneID" id="18869616"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131868"/>
    <dbReference type="eggNOG" id="ENOG502RR5U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_627026_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFW8"/>
    <dbReference type="OMA" id="IWINYSL"/>
    <dbReference type="OrthoDB" id="4075141at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="384"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="433" mass="51603" checksum="7899BA4D512B7F02" modified="2011-11-16" version="1">MSNERQLNRQAYIIRLLKQSPQPLYWIAFLSLNPIIKRLLLDNQDKISQCCKFDTATFERRTKKLANLISCCFLYSATVTNKFIPKDYGLIYFIINYYGELNTPSNTKIQISPNYSKYFKLETYRNHPTLVKLYENKEFFIFPAIFAQLLSNYLTPTRYKLNQRYLSSSIKSRIFAPIWKNFSLGVNHARLNWISLLRNYLIQNYVIIGFLGLLTIKTRLLDRLYEVKYKKRNETELSVVLNYWTYNFHRANSIVNFIYAPNMIAILLITLTSPMFRLLKPKGDIPKNTLQKLYKRNYKLFFKSYTKTIGFVAAFLTLYLNALNVVPALGYKDEEEDETENIRTISKSWLNDLDLYLFRLILLSKWRITKENHPSFKIMKLKNWIRLETLLMCFGVFKVMNLNDYIKKNKQQDPDNYQRLKDNTMIRMVDCIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG35</accession>
    <name>G3AG35_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein CMS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_131035</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372586.1">
        <property type="nucleotide sequence ID" value="XM_007372524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG35"/>
    <dbReference type="FunCoup" id="G3AG35">
        <property type="interactions" value="305"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG35"/>
    <dbReference type="GeneID" id="18869548"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131035"/>
    <dbReference type="eggNOG" id="KOG3089">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086112_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG35"/>
    <dbReference type="OMA" id="CVGTPAR"/>
    <dbReference type="OrthoDB" id="1929311at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032704">
        <property type="entry name" value="Cms1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24030">
        <property type="entry name" value="PROTEIN CMSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24030:SF0">
        <property type="entry name" value="PROTEIN CMSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14617">
        <property type="entry name" value="CMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="30078" checksum="42F9B89284D3F146" modified="2011-11-16" version="1">MAHPTADDLDDGLEYDLQLSDSEEETFVVEQPEVVKEEKEQPANKKRKQNTKFKETKKLKMEMDIETKKNMSVEESPEVIAEYINSKIRRKNPNLSALELADLYFAKSEIRSNVDFKETRNLDNLSAFITNRFKNMLPTKKGKEDSERKFIAIVSMSAIRACDIHRATRELSGSSLKLINKNKLNVDLKLVKSTRSRVLCCTPGRLAKVLAHEDSELQASEVKIIIVDNSYLDSKKQNIWDIPETFDVLKSLTKQGSKIYLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG45</accession>
    <name>G3AG45_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">ATP-grasp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_58377</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the second and fifth step in the 'de novo' purine biosynthesis pathway; contains phosphoribosylamine--glycine ligase (GARS) and phosphoribosylformylglycinamidine cyclo-ligase (AIRS) activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>5-phospho-beta-D-ribosylamine + glycine + ATP = N(1)-(5-phospho-beta-D-ribosyl)glycinamide + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17453"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">In the C-terminal section; belongs to the AIR synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the GARS family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372596.1">
        <property type="nucleotide sequence ID" value="XM_007372534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG45"/>
    <dbReference type="FunCoup" id="G3AG45">
        <property type="interactions" value="1204"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG45"/>
    <dbReference type="GeneID" id="18872425"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58377"/>
    <dbReference type="eggNOG" id="KOG0237">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005361_1_0_1"/>
    <dbReference type="InParanoid" id="G3AG45"/>
    <dbReference type="OMA" id="EVMQACC"/>
    <dbReference type="OrthoDB" id="2018833at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046084">
        <property type="term" value="P:adenine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000006">
        <property type="entry name" value="phosphoribosylamine--glycine ligase, chloroplastic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000001">
        <property type="entry name" value="Phosphoribosylformylglycinamidine cyclo-ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000018">
        <property type="entry name" value="Trifunctional purine biosynthetic protein adenosine-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.600.10:FF:000001">
        <property type="entry name" value="Trifunctional purine biosynthetic protein adenosine-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.650.10:FF:000007">
        <property type="entry name" value="Trifunctional purine biosynthetic protein adenosine-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="entry name" value="Phosphoribosylglycinamide synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020559">
        <property type="entry name" value="PRibGlycinamide_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00877">
        <property type="entry name" value="purD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520:SF12">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02843">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01209">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00184">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="18">
        <location>
            <begin position="9"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW35184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="689" mass="73897" checksum="1072625A2E5DDF6B" modified="2011-11-16" version="1">MEGSKAFSKEFMDKHQIPTARFKNFTDVNKAREHIKNIDYQIVLKADGIAAGKGVLIPQTKEEALQGLDEIMVAKNFGSAGDEIVIEEFLEGDELSILTITDGYSFFNFPAAQDHKRVGDGDTGLNTGGMGAYAPAPIATSEVLATIDREIIKPTIDGMRHDGYPMCGILFTGIMLTKSGPKVLEYNVRFGDPETQTVLPLLSEETDLAQVMLAAAEHRLDSVTLSVKPNMYSTTVVMAAGGYPEAYNKGDVITVKEPLPKDTFIFHAGTAVKDGSVVTAGGRVIAATAIAPSLRESVDKAYVGVEHVSFNNKYHRKDIAHRAFRDADKASSTGGVTYAEAGVSVDNGNLLVEQIKAKVKSTARPGADSDIGGFGGLFDLKAGGYNSDDTLLVAATDGVGTKLRIAQIMDIHDTVGIDLVAMNVNDLVVQGAEPLIFLDYFATGKLDIEIAAKFVSGVADGCIQAGCALVGGETSEMPGMYDEGHYDTNGTAVGAVLKNKILPRVDAMEAGNVLIGLRSDGIHSNGFSLVRKIIEISEYEYSSVAPWSKSGKTIGEEVLVPTKIYVKQLLRSINEDLLLGLAHITGGGLVENIPRALPKTLQAKVDMNTWEVPEIFKWFGKQGKVPYDDILKTFNLGVGMVLIVKNENVDQVLKNLEQDGEKDAIVIGSLVERKAGEPGCVVENVDGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG61</accession>
    <name>G3AG61_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_58402</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372612.1">
        <property type="nucleotide sequence ID" value="XM_007372550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG61"/>
    <dbReference type="FunCoup" id="G3AG61">
        <property type="interactions" value="983"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG61"/>
    <dbReference type="GeneID" id="18872436"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58402"/>
    <dbReference type="eggNOG" id="KOG1364">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021255_2_1_1"/>
    <dbReference type="InParanoid" id="G3AG61"/>
    <dbReference type="OMA" id="PAIFDCQ"/>
    <dbReference type="OrthoDB" id="270602at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02958">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14346">
        <property type="entry name" value="UBA_Ubx5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006577">
        <property type="entry name" value="UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF6">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13899">
        <property type="entry name" value="Thioredoxin_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00594">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="403"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="491" mass="56324" checksum="7B9E2325B05932EA" modified="2011-11-16" version="1">MDEQIPTFLAVTGVEDEAIAKQFLEATGGDLEYAVQLYMEQGQHNTTATSSNAIPNETTPGSDEELAKRLQEEAYAQGEQVREADANIHRHETLVDSLGGGFHPGMANFTRPSDIFGQRQQGIFHQQFNFDNRFEELEDDEEEDHDYYDAHDDEDDIEILDDDSDEEARPVSRRRRLRESRDQELTSTQRRLAALFRPPFDIITVANLDMAKQQGKETSKWILINIQDSSEFQSQVMNRDFWSNEHVKQVVKEFFIFLQYQRDSPNGETYVNFYHADAFPHLAILDPLTGERVYKWQDGEVPKVDEWLSQVDDFLNKFSLLPDSRNPLVTHDVKIDPDSLTEEQQIELAMKQSMLESAGNTENDAIILDSDEEAFAEAPETPPPATPQDPFEAIQPINHAEPDAQPFTRVQIRFPNGKRLVRKLNPSDTIQSLFEWLKYVLQQQGEEFGISSEDKFNLSNSSNKSFKFIESLHQTIEEANLKNASILLEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGE3</accession>
    <name>G3AGE3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chitin synthase export chaperone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_58500</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Chaperone required for the export of the chitin synthase CHS3 from the endoplasmic reticulum.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with CHS3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CHS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372694.1">
        <property type="nucleotide sequence ID" value="XM_007372632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGE3"/>
    <dbReference type="FunCoup" id="G3AGE3">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGE3"/>
    <dbReference type="GeneID" id="18872474"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58500"/>
    <dbReference type="eggNOG" id="ENOG502QRVH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050424_1_1_1"/>
    <dbReference type="InParanoid" id="G3AGE3"/>
    <dbReference type="OMA" id="TVWEVKD"/>
    <dbReference type="OrthoDB" id="2189463at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022057">
        <property type="entry name" value="Chs7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35329">
        <property type="entry name" value="CHITIN SYNTHASE EXPORT CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35329:SF2">
        <property type="entry name" value="CHITIN SYNTHASE EXPORT CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12271">
        <property type="entry name" value="Chs7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="114"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW35282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="314" mass="34824" checksum="1B24CDC29D08D830" modified="2011-11-16" version="1">MTFGSFDSICNKTALPLCSVLGSVNQSSDFQRGILPQCYARSVELANTMIFQVGTAFVHFGGLIIVLIIIFNVRAKYTAIGRTEMLFLLYLFIGLLVSSLIVDCGVSPPSSSSYSYFVAVQLGLASSVCACLLYNGIVCFQFWEDGTRKSMWSLRLICFGWFVVNFIIALITFNHWNTSLDNRNTMVLFIFTYLFNAIIVATYVVSQIVLVVFALDSYWPLGAIIFGVFFFVAGQVLTYVFSHAICSGAAHYVDGIFFGSLCNVFTVMMIYKFWDMITTDDLEFSVANVEQEVPGGAGIGLLDDEKRASSMFFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGL3</accession>
    <name>G3AGL3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial ribosome small subunit component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_133096</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Mitochondrial ribosome (mitoribosome) assembly factor. Binds at the interface of the head and body domains of the mitochondrial small ribosomal subunit (mt-SSU), occluding the mRNA channel and preventing compaction of the head domain towards the body. Probable inactive methyltransferase: retains the characteristic folding and ability to bind S-adenosyl-L-methionine, but it probably lost its methyltransferase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372764.1">
        <property type="nucleotide sequence ID" value="XM_007372702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGL3"/>
    <dbReference type="FunCoup" id="G3AGL3">
        <property type="interactions" value="323"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGL3"/>
    <dbReference type="GeneID" id="18869719"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133096"/>
    <dbReference type="eggNOG" id="KOG2539">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024759_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGL3"/>
    <dbReference type="OMA" id="HRKCPLQ"/>
    <dbReference type="OrthoDB" id="421327at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180026">
        <property type="term" value="P:mitochondrial small ribosomal subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052571">
        <property type="entry name" value="Mt_RNA_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015324">
        <property type="entry name" value="Ribosomal_Rsm22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016522">
        <property type="entry name" value="RSM22_mit_bud"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184:SF5">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09243">
        <property type="entry name" value="Rsm22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007797">
        <property type="entry name" value="RSM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="248"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="547"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="416"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="261"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="747" mass="84968" checksum="F791A610E46F9007" modified="2011-11-16" version="1">MLCSRSIARGNITARLIRQGRCFYSTPKLNESNFDFFTKETRSTTNDNLIKSRQGYSAHSGETIHNTKTVTRSDIIDDKPSHFRNSDGSLIKGTNSDEARLHDSTLEGRVDHRVTRLPKEISKVINNNILGHVVPNKLRERVTSIYKDMQKNSIQRAPVSSLDADAHISAFFLQDYAHARQVLLELQKRVGEEKFNPQRVLSVGYGPAAGMVALNEIMGPDWIPETKDAYIVGRNNGEMKKRAKIILSRQPNENVSASEESAAEPEEPTETNEPEIESEEFVLEEYKGPVDSTKLNIRTKLKNTIPVSKQYDLIIVSSSLLSREFSFPNDVDANIRMLLRVLAPNGHIVLVERGNAVGFETIARARQIMIRPEGYPNENGKIPRPYIHGSSVKPQRVKKDDELITKADIEYEKELLAKWEKEDQELLEEGQELAKELDEKFGEVTEEELKFEDEDDVEVIDPNTQTEAKEEKMDYHLSILAPCPHHQKCPLQMGHPKYYSIPNHSHRFSFCSFSKVVERPKYTMELKKGKLLATSWDKRADDGIGHADRGTLKRLQGGGRPGGRNTEDGSYSYLIAHRSPNDPATIEKIEKDRKFNDKQDFNNVNHWARIIESPNKLKSNVKMSVCASSGKIETWNVPKSLGKQAYHDARKSQEGDLWALAKKGVQVRNIVSDEVREKLDLLYKVNRKKFLKEQHRKSSKKKTGVAIEQFERDSDAIEAAVQKLESTQKYRTGGKKFDVNPDKYEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGT8</accession>
    <name>G3AGT8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ADP-ribosylation factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_132867</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372834.1">
        <property type="nucleotide sequence ID" value="XM_007372772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGT8"/>
    <dbReference type="FunCoup" id="G3AGT8">
        <property type="interactions" value="499"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGT8"/>
    <dbReference type="GeneID" id="18869701"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132867"/>
    <dbReference type="eggNOG" id="KOG0072">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040729_9_3_1"/>
    <dbReference type="InParanoid" id="G3AGT8"/>
    <dbReference type="OMA" id="VRWSKDQ"/>
    <dbReference type="OrthoDB" id="5399569at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031503">
        <property type="term" value="P:protein-containing complex localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04151">
        <property type="entry name" value="Arl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000510">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="129"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW35422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="185" mass="20660" checksum="913157C681674D3A" modified="2011-11-16" version="1">MGQAFSFANIFGKLWGVQKEIRILILGLDGAGKTTILYRLQMGEVVTTKPTIGFNVETLKYKNVTLNIWDLGGQTSIRPYWRCYYSNTSAIIFVVDSTDKDRIDTACKELHTMLKEEELQDSALLVFANKQDQPGAMTAAEVSQALDLTDLKDRSWSIVASSAIKGEGLTEGLDWLMDVIKDEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH62</accession>
    <name>G3AH62_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58734</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372904.1">
        <property type="nucleotide sequence ID" value="XM_007372842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH62"/>
    <dbReference type="GeneID" id="18872577"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58734"/>
    <dbReference type="HOGENOM" id="CLU_1543781_0_0_1"/>
    <dbReference type="InParanoid" id="G3AH62"/>
    <dbReference type="OrthoDB" id="4026683at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="174" mass="20590" checksum="E41E0459DD520AE5" modified="2011-11-16" version="1" fragment="single">MHQEDQEDSPPDYSSFIEEEELPTYKQLFCFKGWKFVIKNCFGDSSHIFISKRSSKRYHQYCLSPTSSSSSSSPVLQNRGCDIPMFIVSGAISNARPLVFKKYNDTIDHRDHPFDLDNDYYTFCTVYRHCFLKYSSFRFRFTTDPNNKHNVFEIIMFSHNTLPISDYIYNGERF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH68</accession>
    <name>G3AH68_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_58739</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372910.1">
        <property type="nucleotide sequence ID" value="XM_007372848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH68"/>
    <dbReference type="FunCoup" id="G3AH68">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AH68"/>
    <dbReference type="GeneID" id="18872579"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58739"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_22_3_1"/>
    <dbReference type="InParanoid" id="G3AH68"/>
    <dbReference type="OMA" id="GIANIWY"/>
    <dbReference type="OrthoDB" id="3437016at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF191">
        <property type="entry name" value="VACUOLAR BASIC AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="293"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="326"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="403"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="427"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="460"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="496"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="572"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="98"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="602" mass="64932" checksum="33335A613D5C82ED" modified="2011-11-16" version="1">MFNSQDNDHSEELHSSFTHPHLHPNIIQDSSAGSAGIVAGELAAEDEGLLNNELFRSHSYGAIPEEQEAAEESHEPSKYDIDDPNGGYALSKRQLYPVVASIFMGAYLAALDATVVTTLLTLIASDLDAVSNISWIATAYLLSCSAFQPIFGKLSDIFGRKVLLVCCTILFGVGCLICVTDSLAILVIGRFITGWGGSGLTSLGTITMSDIIPLRDRGFYQGLANVFFGLGSASGGIIGGLVADYLGWKYVFILQVPLAFIVGLVIHLNLNLPEGSPGLGAQGQDIKQKLKRVDFLGSFVLVTSLMMVLTAASLGGREIAYSSKTFIGLTATSLILLGGFVYVEMNVSTEPIIPIHLLANRTILASSLTNWFYTMGIFTTLFYVPVYYTSVLDYTATQNGVRLIPNFLGVSLGSVGAGLYMKKTGKYYKLAVVSGIVSLFGMTKIAIITPSIPNWQQFLLLLPSGLGYSSILTVTLLALIAAVPMKYQACTTSIQYTFRSTGSTLGVSIASAIFQNVLLHRLTDSVNKLVPDKDQAKDIIKHALKNTNYIDLAPKYVRKAIRESYAAGCKGAFIFGLSTVAIGYVCSLFMREHKLHTSMNRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHC9</accession>
    <name>G3AHC9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_148495</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373677.1">
        <property type="nucleotide sequence ID" value="XM_007373615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHC9"/>
    <dbReference type="GeneID" id="18870787"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_148495"/>
    <dbReference type="eggNOG" id="ENOG502RQUH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_512992_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHC9"/>
    <dbReference type="OMA" id="FESIMEH"/>
    <dbReference type="OrthoDB" id="4025768at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="108"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="511" mass="59450" checksum="67B3E622A55490DC" modified="2011-11-16" version="1">MSSNNRNNDDTTHTDASILSQLRQYQIQSRLDDELNERCRHQIDSIFEYTDLLIEGHDKEFDKQLVYDGMDESLPIPSTATLTAFAIQLNKLRILHLKLKTDAMNVSKERIKGKIEVLKEKKQEQEKKLDQLRLDLLKSETKLISNGNARIDSLNSQIEENKSTKNKQVGRQVLRLQEQNFKILKEISFQNKERKLLFNHQPILKLEELLGYNLLVINQFLERLIVLQIQLSDLFRVELPHLPELVGFLPDSKFYDLIRKKQQIIKGSEEDGLSDVESSTDTPTSEDSLIFEVPKDIQNPTEKIIKLGDAYKLPLSSKTLNHQRRAHRSNSVEPADLSNIPIIKHDRSVSPSTSSPKKNMVIIPHKIINKPFNRLSIKDFLRFFLVIVKLITNFQVFLMFTGEIEDMSLDEWCNFEKVLRQVMNLDLGFKKRLYEQEKQAVDYATSAVRTNFQDLMEHVYELLMKSSAVLGTKKKHSDKPMAFLDLKLRDIIRNPTRLNLSEEWDIVSHMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHQ1</accession>
    <name>G3AHQ1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein bL32m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_135524</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373799.1">
        <property type="nucleotide sequence ID" value="XM_007373737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHQ1"/>
    <dbReference type="FunCoup" id="G3AHQ1">
        <property type="interactions" value="290"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHQ1"/>
    <dbReference type="GeneID" id="18869896"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135524"/>
    <dbReference type="eggNOG" id="KOG4080">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095763_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHQ1"/>
    <dbReference type="OMA" id="IRHIWKT"/>
    <dbReference type="OrthoDB" id="2014905at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051991">
        <property type="entry name" value="Mitoribosomal_protein_bL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002677">
        <property type="entry name" value="Ribosomal_bL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21026">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21026:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL32M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01783">
        <property type="entry name" value="Ribosomal_L32p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="198" mass="22830" checksum="903DB217AAA8C6C6" modified="2011-11-16" version="1">MSLAFRFGAIAIAHESLLNIIPRLPAISIRIGETVSEAPASEIDPRLEELAEHLHNNDGEPPFMIDNGILRAVPKKKMSYRRHRTKLYAPGNKQVQPLHNIVRCSACGRVKRSHFMCMYCFAEIRTFLKGLKRENGIIKDAVDPQADIDPTDARILYPGKNISAHEHQLKKKDYVPKREEPLMFDKEKLMREKKKWYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHX6</accession>
    <name>G3AHX6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein KRE1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_59707</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373874.1">
        <property type="nucleotide sequence ID" value="XM_007373812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHX6"/>
    <dbReference type="STRING" id="619300.G3AHX6"/>
    <dbReference type="GeneID" id="18873039"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59707"/>
    <dbReference type="eggNOG" id="ENOG502SFZS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156717_1_0_1"/>
    <dbReference type="InParanoid" id="G3AHX6"/>
    <dbReference type="OMA" id="MGIQSSI"/>
    <dbReference type="OrthoDB" id="5406216at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031452">
        <property type="entry name" value="Kre1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17056">
        <property type="entry name" value="KRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442529" description="Protein KRE1" evidence="2">
        <location>
            <begin position="19"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="13490" checksum="AE10E2D8455D46D0" modified="2011-11-16" version="1">MKFNFFPIFFICISLVLAADAPTTTASPTLVWVTGTDANGILRTTQSPYTQQFSTFFTSVAAPSSGVIGLGTISGSVGEVRTYDIVTITHNGGGASNFALGFGDNMEVSAIKYLGLSLFAFISLVLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHZ1</accession>
    <name>G3AHZ1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Mitochondrial escape protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_59728</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Plays a role in maintaining the mitochondrial genome and in controlling the mtDNA escape. Involved in the regulation of mtDNA nucleotide structure and number. May have a dispensable role in early maturation of pre-rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
            <topology evidence="1 10">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the YME2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373890.1">
        <property type="nucleotide sequence ID" value="XM_007373828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHZ1"/>
    <dbReference type="FunCoup" id="G3AHZ1">
        <property type="interactions" value="156"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHZ1"/>
    <dbReference type="GeneID" id="18873049"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59728"/>
    <dbReference type="eggNOG" id="ENOG502QS0P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007861_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHZ1"/>
    <dbReference type="OMA" id="FSHPRIT"/>
    <dbReference type="OrthoDB" id="10267654at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018850">
        <property type="entry name" value="Mt_escape_2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039627">
        <property type="entry name" value="Yme2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32198">
        <property type="entry name" value="MITOCHONDRIAL ESCAPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32198:SF2">
        <property type="entry name" value="MITOCHONDRIAL ESCAPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10443">
        <property type="entry name" value="RNA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442343" description="Mitochondrial escape protein 2" evidence="11">
        <location>
            <begin position="22"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="Mitochondrial escape protein 2 C-terminal" evidence="12">
        <location>
            <begin position="91"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="571" mass="65407" checksum="8E165C1351BA75CE" modified="2011-11-16" version="1">MPILLALLATAAVLIFDPIRQWFIEYKINHSNESYKKSKWFRMIYVPYTSIRDWISISYDYYIDTPDEENLSEKQQPTKIEPEDNLFWYERMEKSKQVRLWIKENTNTFIIVKGPPGSGKEEFILEHTLHNDDKLNKRVLIIECDQLAKARSDNALFKNSASQLGYFPVFTWTNNLSRFIDLGIQGLTGQKSGLSESKETRVKGMFSLSIQAIRHITDKEYSNYVKSVERANRGKPENAKVEILNQEDFLQQHPECKPIVVVTKFARKADTAGNDFIFPMIAEWSSNLVQNNIAHVIFSTADVGSLQHLNDALPNQVFKDISLSDASMTSSKQYVCEQLKLAEGKTANIENCIEPIGGRMLDLQAFVRRVKSGETPNIAITEMINQASELITSFFLENHKIEQGDHNWTTAQVWVLMKMLSKSDKVKYNDLIKSPLFGSSPDTVNTLSTLEKYDLVALKRDKGVLNEITTGRPLFKAAFRNIIKDVYIWKLYETNYLSSLVKIEVDNIQKLENELVQISKLEGKLNGRIDYLVKKIDASTTKIINYEKQISEVGQFTGDKHQKSSSFLGIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI13</accession>
    <name>G3AI13_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chromatin associated protein KTI12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_59742</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the KTI12 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373911.1">
        <property type="nucleotide sequence ID" value="XM_007373849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI13"/>
    <dbReference type="FunCoup" id="G3AI13">
        <property type="interactions" value="670"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI13"/>
    <dbReference type="GeneID" id="18873059"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59742"/>
    <dbReference type="eggNOG" id="KOG3062">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027147_1_0_1"/>
    <dbReference type="InParanoid" id="G3AI13"/>
    <dbReference type="OMA" id="ELWCIAC"/>
    <dbReference type="OrthoDB" id="9972657at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013641">
        <property type="entry name" value="KTI12/PSTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12435">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08433">
        <property type="entry name" value="KTI12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="263" mass="30127" checksum="51F87FC9951E74C4" modified="2011-11-16" version="1" fragment="single">EQLKTKIQSAKDKGEVGQNYDVIYHSDETLGISHEVYSDSTLEKNARGTQISAVKRDISRSNIVILDSMAYIKGFRYQLFCEAKATVTPHCVVHVIAPVEQCLEWNNNREDKWDEELIKQLVMRYEEPNSDTRWDSPLFTIASDDPKETLPIDEIWDTLVLKRPPPPNAATLIKPTSGNSFLQELDKKTQEVITKILQHQQITTGDVIIDKDLVVQLPATSVSTAQLQRIRRSYISLNRMRSVETDRIIPLFVDYINRSLNND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI45</accession>
    <name>G3AI45_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_59778</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373943.1">
        <property type="nucleotide sequence ID" value="XM_007373881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI45"/>
    <dbReference type="FunCoup" id="G3AI45">
        <property type="interactions" value="796"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI45"/>
    <dbReference type="GeneID" id="18873074"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59778"/>
    <dbReference type="eggNOG" id="KOG0569">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_5_1"/>
    <dbReference type="InParanoid" id="G3AI45"/>
    <dbReference type="OMA" id="WAITASF"/>
    <dbReference type="OrthoDB" id="4540492at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015149">
        <property type="term" value="F:hexose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF8">
        <property type="entry name" value="FACILITATED GLUCOSE TRANSPORTER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="273"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="356"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="474" mass="51877" checksum="D7CE080E39F05E58" modified="2011-11-16" version="1">MAELNSPELVLSCRRSVPGDIPFADSWFGTHGFVQCIPMSPSQIGLATSIFTIGGLFGSFYVGHLADKFGRKNTSLLHCFIYFIGSFINGMSNSFAWLLIGRFIVGLAAGSALVITSLYINEVAPVNAKGLLGSMNQVSINCGILFTQLLSLKWANDNQWRYLLFMASALGFVNLVLLYVYGLESPVWLANNGRSTEAFTVLHRIRGGSYSVATDEVNSWKNNHRGNITETDSGLENGPGNMAIDDSTKRIVTVKEYVTSPEFKNSRTVATGILVLQQFDGINSIIFYGVSVLVSIFPNHAIVINCLISLVNVVITFASATVVDRLGRKPLLLTSVSFLFISTILMGIGIMSTNSVLSIVGTFTYITFFAIGLGPIPFLLVGEVTQPIAKASAQSWGTSMNWVATFIVGYSFPVLIHKFGGSIYFLFSFMCLVSFWFIKQYIPETKGKDSYEQVWGGFIGSTNGYSSHVETEHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIA2</accession>
    <name>G3AIA2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anaphase-promoting complex subunit 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_135028</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373256.1">
        <property type="nucleotide sequence ID" value="XM_007373194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIA2"/>
    <dbReference type="FunCoup" id="G3AIA2">
        <property type="interactions" value="271"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIA2"/>
    <dbReference type="GeneID" id="18869862"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135028"/>
    <dbReference type="eggNOG" id="KOG1493">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115512_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIA2"/>
    <dbReference type="OMA" id="TWHWKLA"/>
    <dbReference type="OrthoDB" id="1681166at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16456">
        <property type="entry name" value="RING-H2_APC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000469">
        <property type="entry name" value="Anaphase-promoting complex subunit 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051031">
        <property type="entry name" value="RING-box_E3_Ubiquitin_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024991">
        <property type="entry name" value="RING-H2_APC11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11210">
        <property type="entry name" value="RING BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12861">
        <property type="entry name" value="zf-ANAPC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="43"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="139" mass="15896" checksum="4FC1774A556E37F5" modified="2011-11-16" version="1">MKVKILEWKSFATWHWDLATSADDSGYVEELCGICRVSFDGTCPNCKYPGDDCPIVLGSGCTHNFHLHCILKWLEQESSKGLCPMCRQIFTFKEPEQTSDVLINLKTLIDGHRVMRERFQQGNEQEFEAFGADQDLQMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIC1</accession>
    <name>G3AIC1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">DNA replication complex GINS protein SLD5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_59059</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The GINS complex plays an essential role in the initiation of DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the GINS complex which is a heterotetramer of SLD5, PSF1, PSF2 and PSF3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GINS4/SLD5 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373275.1">
        <property type="nucleotide sequence ID" value="XM_007373213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIC1"/>
    <dbReference type="FunCoup" id="G3AIC1">
        <property type="interactions" value="644"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIC1"/>
    <dbReference type="GeneID" id="18872748"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59059"/>
    <dbReference type="eggNOG" id="KOG3176">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071893_2_0_1"/>
    <dbReference type="InParanoid" id="G3AIC1"/>
    <dbReference type="OMA" id="ILETAWI"/>
    <dbReference type="OrthoDB" id="60955at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11711">
        <property type="entry name" value="GINS_A_Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21692">
        <property type="entry name" value="GINS_B_Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008591">
        <property type="entry name" value="GINS_Sld5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031633">
        <property type="entry name" value="SLD5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038749">
        <property type="entry name" value="Sld5_GINS_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21206:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN SLD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21206">
        <property type="entry name" value="SLD5 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16922">
        <property type="entry name" value="SLD5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007764">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160059">
        <property type="entry name" value="PriA/YqbF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GINS subunit" evidence="8">
        <location>
            <begin position="68"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="DNA replication complex GINS protein SLD5 C-terminal" evidence="9">
        <location>
            <begin position="170"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR007764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="222" mass="25566" checksum="4ED98586672FC1E8" modified="2011-11-16" version="1">MNIDDILQEFEESETPVTSNIHNNLITAMINERMSPELLPFAQTTMTTALTAISNQQQYLIDSHEYGDSVGSTSEFKLNTMIIETEIERLCYLVRMYLRTRLSKLDKFTIYYINEEQEDSKLLSAEEKEYIHKYAHLLTQLYNNCFLKKVPPSLTLLDDTSGGQSMITTPDTNQHVFVKSVTSKPITLFIDNDDIELKRDGIYVVKYGLVKQYIELGDVILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIU0</accession>
    <name>G3AIU0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome assembly chaperone 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59115</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373335.1">
        <property type="nucleotide sequence ID" value="XM_007373273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIU0"/>
    <dbReference type="FunCoup" id="G3AIU0">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIU0"/>
    <dbReference type="GeneID" id="18872780"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59115"/>
    <dbReference type="eggNOG" id="ENOG502RY9B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082454_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIU0"/>
    <dbReference type="OMA" id="PCQLNNN"/>
    <dbReference type="OrthoDB" id="3980818at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12120">
        <property type="entry name" value="POC1 chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038605">
        <property type="entry name" value="Pba1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018855">
        <property type="entry name" value="Psome_chaperone_1_fun"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10450">
        <property type="entry name" value="POC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="261" mass="29004" checksum="BA1F4005E06EBA70" modified="2011-11-16" version="1">MLIKPINDIRNPRHILDDDTDDTTPHPIPTVSIDLQTSSIGNIVVIPKTISILQDELSSLTQVGTVKIEYPELVQTTSEESQLDYDEDEQLYSAIRQRELDTKYPTAQIPIYSISNGNVVLTVPHFYNTIAYNVLARQLVKVGNPKQWILLSPCSMNNNQTIAILESNPLLEVIPVLTPPHTVTGISAAIISQLPPSAKLIPLVLNSEGQPGFEKSDNDAIIDICVILGEVLQIEDKEDYLKRVSSKVRKFNGYSNLGMYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIU5</accession>
    <name>G3AIU5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_134377</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the GLYK kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373340.1">
        <property type="nucleotide sequence ID" value="XM_007373278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIU5"/>
    <dbReference type="FunCoup" id="G3AIU5">
        <property type="interactions" value="341"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIU5"/>
    <dbReference type="GeneID" id="18869807"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134377"/>
    <dbReference type="eggNOG" id="KOG2878">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056986_1_0_1"/>
    <dbReference type="InParanoid" id="G3AIU5"/>
    <dbReference type="OMA" id="FWRSLHP"/>
    <dbReference type="OrthoDB" id="347435at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001691">
        <property type="entry name" value="Probable ATP-dependent kinase TDA10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="292" mass="33398" checksum="ECAEEB83E3910CD7" modified="2011-11-16" version="1">MTSLDLSIGFISKEIDSCDFSNVNKPIVVGISGPQGSGKSYLANHLTAALQKSYPHLNIIHFSMDDLYLTKKDQDEVTKASIESENKLLQGRGLPGTHDLPLALQIFHALINNYSVSPWKSVEIPFYDKTAYHGLGDRADKSQWQIIDTPADVIIFEGWFNGFVPLGREQVDATYFTSEVGGVLQKSRYYHIQEINDNLQPYTKLWSFFDKFIVLCTNSIDNVYTWRLQQEKELIKQKGSGMTDEQVEKFVDRYMPMYILYYEHLCSNGLDHCSNLKISIDLNRNVVETRQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIU7</accession>
    <name>G3AIU7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HDA1 complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_70038</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373342.1">
        <property type="nucleotide sequence ID" value="XM_007373280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIU7"/>
    <dbReference type="FunCoup" id="G3AIU7">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIU7"/>
    <dbReference type="GeneID" id="18875301"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70038"/>
    <dbReference type="eggNOG" id="ENOG502QT9V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026579_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIU7"/>
    <dbReference type="OMA" id="INYSGDY"/>
    <dbReference type="OrthoDB" id="3647690at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070823">
        <property type="term" value="C:HDA1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038609">
        <property type="entry name" value="HDA1_su2/3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021006">
        <property type="entry name" value="Hda2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026216">
        <property type="entry name" value="HDA3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11496">
        <property type="entry name" value="HDA2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02093">
        <property type="entry name" value="HDA1SUBUNIT3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="426"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="561"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="178"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="653" mass="75721" checksum="1D54F2ECA8211DE8" modified="2011-11-16" version="1">MNLLKILDGTPEPSIIDLDLGEINYSGDYELPTPMYEFQKELTDQIVSLHYPDILKYCEANDTRDLIVKSLEICIENCMLVCTHPYLLINHYMPKNLSMKEMPNKLAETSGKFNVLRDLMNVIISNTKQRPKHIGIVMNNNGKFFDLVEALLLGCNGNKIVKRYVGNNVQRESKKASRNSGTPMDMSGSPTTIHLLPGDGKIQRDQDRLGLVKFDALIVFDGYVDTDSEFFQQIRTQNRRGECIIVRLVPMKTIEHCKLYYKNNAGDDDYIYKLISSIVCLRDHIGTLQPDVFPIYNQKLTYLSHKFFDDVFKSSARIADFPAWPLPALPRIPKFNAIDVERSLLTEVHFHYTPYDSTEFINEDPEKPRTGSRPSFYETRRLESAYITNPLKNSFTQLSGIYAGTGEEHASATNTSILTHKLILQMNEAFLSLDRAEEEVQSYEKYNEEENQERKIGRREREMRITVSKIFNDVDHAQSRINSASKWTIKKAEQAESVKDEIRVADEKISNFITDNKIDNEQTRQYIQNQLEIWDLQTKVKTLIAKIQSKNEEKNFISNEYQQCLESIKNSETEIENQKTKIQDSKRKYSELLEAEEHEMENFHAKKKDLINQIKEERLQNESLKFKLNNAFKFLKDTSHLKKRKGRGSTPIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIW9</accession>
    <name>G3AIW9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Lysophospholipase</fullName>
            <ecNumber evidence="2 10">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_48897</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the release of fatty acids from lysophospholipids. Phospholipase B may well contribute to pathogenicity by abetting the fungus in damaging and traversing host cell membranes, processes which likely increase the rapidity of disseminated infection.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the lysophospholipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="2 10"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373364.1">
        <property type="nucleotide sequence ID" value="XM_007373302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIW9"/>
    <dbReference type="FunCoup" id="G3AIW9">
        <property type="interactions" value="106"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIW9"/>
    <dbReference type="GeneID" id="18871283"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48897"/>
    <dbReference type="eggNOG" id="KOG1325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014602_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIW9"/>
    <dbReference type="OMA" id="TEDANWP"/>
    <dbReference type="OrthoDB" id="4084751at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000010">
        <property type="entry name" value="Lysophospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF33">
        <property type="entry name" value="LYSOPHOSPHOLIPASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9 10" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6 9 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005131570" description="Lysophospholipase" evidence="10">
        <location>
            <begin position="19"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="PLA2c" evidence="11">
        <location>
            <begin position="35"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW33780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="580" mass="64401" checksum="2809136169C98EAE" modified="2011-11-16" version="1">MISNTLLLLSLYFCLTRSLSLFSTGGGSYGPTSSSCPANRIGTRLANSLSQEEADWVNKRNKITDQNLIDFLKHANMQDFDPESYIKSLDRSIKIGLAFSGGGYRAMLCGAGQFAALDKRTQGAWENGLGGLVQSSTYTVGLSGGSWLVGTIALNEFTDVDQIIRENKLWNLAKPVFMPGGLNILETLSVLTKVELDVLQKWTSGFGVSITDVWGRVLSYQFFNKLKDFGAGLTWSSITNTNAFANHNMPFPIVIALGREPGTYIISTNSTVIEFNPYEMGSWDPTLYQFTPLQYLGTEYNNGRPRGTCVTGFDNAGFIVGTSSSVFNLVFLKILQTLPWPVSTILAKISDLLDRKDDIAIYSPNPFRNTRAAAKKISGSDTLYLVDGGEDSQNIPFAPLLQPERDVDVIFAYDNSADDSNSWPNGAAIRATFQRQFNKQGLNASMFPYVPDASTFENLKLNSKPMFFGCNAKDLIKLQVTTKNPFSIYSSPLIVYTANRKFSYSSNTPTFQLIYLNSDRNMMIQNGFEVASRLNSTLDSEWQACVGCAIIRREQERQGQEQTEQCKRCFKKYCWNGPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIZ9</accession>
    <name>G3AIZ9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">F-actin-capping protein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_59946</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the F-actin-capping protein alpha subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374096.1">
        <property type="nucleotide sequence ID" value="XM_007374034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIZ9"/>
    <dbReference type="FunCoup" id="G3AIZ9">
        <property type="interactions" value="833"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIZ9"/>
    <dbReference type="GeneID" id="18873146"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59946"/>
    <dbReference type="eggNOG" id="KOG0836">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045161_3_0_1"/>
    <dbReference type="InParanoid" id="G3AIZ9"/>
    <dbReference type="OMA" id="VACIEDH"/>
    <dbReference type="OrthoDB" id="423283at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008290">
        <property type="term" value="C:F-actin capping protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110085">
        <property type="term" value="C:mitotic actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.60">
        <property type="entry name" value="F-actin capping protein, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.210">
        <property type="entry name" value="F-actin capping protein, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002189">
        <property type="entry name" value="CapZ_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037282">
        <property type="entry name" value="CapZ_alpha/beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042276">
        <property type="entry name" value="CapZ_alpha/beta_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042489">
        <property type="entry name" value="CapZ_alpha_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017865">
        <property type="entry name" value="F-actin_cap_asu_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10653">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10653:SF0">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01267">
        <property type="entry name" value="F-actin_cap_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00191">
        <property type="entry name" value="FACTINCAPA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90096">
        <property type="entry name" value="Subunits of heterodimeric actin filament capping protein Capz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00749">
        <property type="entry name" value="F_ACTIN_CAPPING_A_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0117">Actin capping</keyword>
    <keyword evidence="4 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="256" mass="28988" checksum="02C5D28CD10E7E6F" modified="2011-11-16" version="1">MSVKLNQLVDSLVKTSPPGELPQINNSLSALTHESIDQALEKYINENSSVFSGYIASQYNKDANSSKYIDYVSKKMFNIDTKSFKAIDIESYTAHADYPSYFDELVKKLETYGEDHYPQFAFTVIPGDVVRVIIIGRRLNPDNFYTGQWKSEYAIQNGQISGTVNLDIHYYEDGNVRLKLDEKLDGESVGGSASDIVNFISNSETKVTLKIIDQFNDLNQQQFKNLRRLLPITRSKINWGNAIGNYRLGSDVVNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJM3</accession>
    <name>G3AJM3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_133535</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373508.1">
        <property type="nucleotide sequence ID" value="XM_007373446.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AJM3">
        <property type="interactions" value="1340"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJM3"/>
    <dbReference type="GeneID" id="18869756"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133535"/>
    <dbReference type="eggNOG" id="KOG1795">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000380_3_0_1"/>
    <dbReference type="InParanoid" id="G3AJM3"/>
    <dbReference type="OMA" id="ANKWNTS"/>
    <dbReference type="OrthoDB" id="1931567at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097157">
        <property type="term" value="F:pre-mRNA intronic binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000386">
        <property type="term" value="F:second spliceosomal transesterification activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000389">
        <property type="term" value="P:mRNA 3'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000395">
        <property type="term" value="P:mRNA 5'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08056">
        <property type="entry name" value="MPN_PRP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13838">
        <property type="entry name" value="RNase_H_like_Prp8_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.40:FF:000001">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.230:FF:000001">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.43.40:FF:000001">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000002">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1570.40:FF:000001">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1570.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.40">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012591">
        <property type="entry name" value="PRO8NT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012592">
        <property type="entry name" value="PROCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012984">
        <property type="entry name" value="PROCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027652">
        <property type="entry name" value="PRP8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021983">
        <property type="entry name" value="PRP8_domainIV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043173">
        <property type="entry name" value="Prp8_domainIV_fingers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043172">
        <property type="entry name" value="Prp8_domainIV_palm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019581">
        <property type="entry name" value="Prp8_U5-snRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042516">
        <property type="entry name" value="Prp8_U5-snRNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019580">
        <property type="entry name" value="Prp8_U6-snRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019582">
        <property type="entry name" value="RRM_spliceosomal_PrP8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11140">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR PRP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11140:SF0">
        <property type="entry name" value="PRE-MRNA-PROCESSING-SPLICING FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08082">
        <property type="entry name" value="PRO8NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08083">
        <property type="entry name" value="PROCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08084">
        <property type="entry name" value="PROCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12134">
        <property type="entry name" value="PRP8_domainIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10598">
        <property type="entry name" value="RRM_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10597">
        <property type="entry name" value="U5_2-snRNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10596">
        <property type="entry name" value="U6-snRNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="MPN" evidence="8">
        <location>
            <begin position="2162"/>
            <end position="2288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="2390" mass="277670" checksum="D0BB97A51687DCD5" modified="2011-11-16" version="1">MGRKLPPPPPPPPGINSDSVNGNARKRTKPGPPPPPPPPTSNLQPKTQPPPPPPPPSRAVPPPPPPPPSSIVTPSSSQQELDSRKRQWLQIQKTRFKQAPNKASGGIIHPHKPEMPPQHLRKIMLDHGDLTTKRIASDKRSHLGSLKYLPHALLKLLENMPQPWEQSKEVKVLYHTTGAITFVNEIPRVIEPVYIAQWSNVWNQMRQEKRDRRHFKRIRFPPFDDEEPPIDWNENLEGVDPPDSIRGEHEEWFFEQQPFIDDEDVVNGDSYRKWNLDTTNMHKLYQTSKPILNETQAAPYLFDNDAFITAKSLNVALPGGPKFEPRYNKEDIPEDDDFTEFNYIDRIIFRIPIRTEYKVAFPYLYNSFVKSVQLISYHHSINCYQANKQDTLVYTFNANFNPILPRVKPKQQQVQEEEEEIFELDVEPFLKWNSEESEESDEEELEEELPFEPQGTKQALELLWAPYPFNRRSGKTVRAQDVALIKSWYKRHAPRKAPAKVRVSYQKLLKNHILNQVHTRKRRHTPKRAKLLPSLKSTKYFHQTTIDWVEAGIQVCRQGFNMLNLLIHKRGLTYLHLDYNFNLKPTKTLTTKERKKSRFGNAFHLIRELLRMIKIIVDSHVQYRLGNIDAFQLADGLYYIMNHLGQLTGIYRYKYKVMHQIRQCKDLKHLVYHRFNNIIGKGPGCGFWQPAWRVWIFFLRGVVPLLERWLGNLIARQFEGRRANDVAKTITKQRVDSYYDLELRAQVMHDILDMIPEGLKQSKSKTILQHLSEAWRCWKANIPWKVAGLPEPIEKIIERYIKAKADGWISVTHYNRDRIQRGATVEKTIAKKNLGRLTRLWIKNEQTRQMNFAKNGPYVSPDEAVTIFQTMINWLESRKFSPIPFPPINYKHDTKLLILALEGLKSSYNVNSKLNSQQREELALIEQAYDNPHECLARIKKYLLTQRIFKEVGLEMMDYYSHIVPTYAVDPLEKITDAYLDQYLWYEADKRKLFPNWIKPSDDEIPPLLVYKWCQGINNLHQVWDIDQGQCNVMLETSLEKFAEQIDFTLLNRLLRLVMDTNIADYITAKNNVNLTFKDMNHINQYGLIRGLQFSSFVYQYYGLVIDLLILGLDRANELAGPITSPNNFAQFKDLTTETSSPIRLYSRYMDKIHIFFRFDNEESTDLIQDYLSENPDPNFENVVGYNNHKCWPRDSRMRLMRHDVNLGRAVFWEVQGRIPKSLTSIEWENTFASVYSRDNPNLLFSMCGFEVRILPKVRNIGGSSIESSWDLIDQTTKERTAKAYLQVSQDSIDNFNNRIRQILMSSGSTTFTKIAAKWNTALIALVTYFREAAISTPTLLDVLVKCETKIQNRVKMGLNSKMPSRFPPAVFYTPKELGGLGMLSASHVLIPASDLRWSTQTDTGITHFRAGMTHEEDKMIPTIFRYVTSWENEFLDSQRVWAEYAIKRQEALDQNRRLTFEDMENNWDRGIPRISTLFQKDRHTLAYDKGHRIRNQFRQFSLARFNPFWWTSNHHDGKLWNLNNYRTDVIQALGGIETILEHTLFKGTGFDSWEGLFWEKASGFEDSLKFKKLTNAQRSGLSQIPNRRFTLWWSPTINRANVYVGFLVQLDLTGIFLHGKIPTLKISLIQIFRAHLWQKIHESIVSDLCQVFDKELEVLQIDNVEKQPIHPRKSYKMNSSTADIVCISTYKWKLSKPSLLNEDDRDSYPITANKFWIDVQLRYGDYDSHDISRYARSKFLDYTTDGTSSYPSPTGIIIAIDLAYNMYDVYGNWFPGLKPLIHNAMKEIMKSNPALYVLRERIRKGLQLYQSQPQQPLLNSNNYAELFNNETQFFIDDTNVYRVVVHKTMEGNLATKPINGCIFILNPKTGQLFLKIIHTSVWSGQKRLGQLAKWKTAEEVTALVKSLPREEQPKQLIVTRKGMMDPLEVHMLDFTNISIRSSELHLPFGAVMKINKLNEIVLKASEPQMVLFNFYDDWLKTISPYTAFSRVILILRAMNIDQETTNRILQPDASIIVQDHHIWPSLSDETWIDVESKLRDLILDDYSKKYNVNIQSLTQSEIRDLILGQDIRAPSAKRQEISQIESAPNNVENKDLVALKTTTTNVHGEEIVTVTTTNYEQSTFSSRNEWRNRAIAANSLYLRTNNIYVNSSDFEDESSYTYILPKNILSKFVQISDLRIQVGGFLYGKSPSDNNQIKEIKCIAIVPQLGNVNSIQFPEHLPESVGYLQDLELLGWVHSQSQDFSYMTSYDVTTQAKFNIPSNFIDMTVAFTPGSITISAFELNELGYEWGKQNQDMISESPQGFSKDFSKQGQLIVTDQIGGTFMVPEDEIWNYFFIGTMFNPKEIYDMKVDIPIGFYHELHRPIHFLGFSQLQEDNIAEADQEDVFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJZ2</accession>
    <name>G3AJZ2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Agglutinin-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_135549</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ALS family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373627.1">
        <property type="nucleotide sequence ID" value="XM_007373565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJZ2"/>
    <dbReference type="FunCoup" id="G3AJZ2">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="GeneID" id="18869900"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135549"/>
    <dbReference type="eggNOG" id="ENOG502RGCG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031316_2_0_1"/>
    <dbReference type="InParanoid" id="G3AJZ2"/>
    <dbReference type="OMA" id="APQCENG"/>
    <dbReference type="OrthoDB" id="3981162at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903561">
        <property type="term" value="C:extracellular vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030446">
        <property type="term" value="C:hyphal cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030445">
        <property type="term" value="C:yeast-form cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043710">
        <property type="term" value="P:cell adhesion involved in multi-species biofilm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043709">
        <property type="term" value="P:cell adhesion involved in single-species biofilm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030448">
        <property type="term" value="P:hyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044011">
        <property type="term" value="P:single-species biofilm formation on inanimate substrate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2430">
        <property type="entry name" value="Agglutinin-like protein, N-terminal domain, N2 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008966">
        <property type="entry name" value="Adhesion_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008440">
        <property type="entry name" value="Agglutinin-like_ALS_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024672">
        <property type="entry name" value="Agglutinin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043063">
        <property type="entry name" value="Agglutinin-like_N_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033504">
        <property type="entry name" value="ALS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011252">
        <property type="entry name" value="Fibrogen-bd_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793:SF2">
        <property type="entry name" value="AGGLUTININ-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793">
        <property type="entry name" value="ALPHA-AGGLUTININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05792">
        <property type="entry name" value="Candida_ALS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11766">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01056">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49401">
        <property type="entry name" value="Bacterial adhesins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0134">Cell wall</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 16" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0843">Virulence</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442399" description="Agglutinin-like protein N-terminal domain-containing protein" evidence="16">
        <location>
            <begin position="18"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Agglutinin-like protein N-terminal" evidence="17">
        <location>
            <begin position="54"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW34043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="455" mass="48539" checksum="FB57C418A491A945" modified="2011-11-16" version="1" fragment="single">MFRFLIFTILTFAIVSAEQISGIFTSFDSLVWQQSGTYPYTNGPGFASWIATLSWKIDGSTVSPGDTFTLNLPCVFKFTSTTPTVNLTVGSVNYATCTFNPGDVVVSFSQLQCVLLDTVTPSLDANGSINFPIVFNVGGSALNTDLQDSTCFHSGQNTVSFYDGNNQLSTQATFNNGSFTDPNSIVFNNRAVPSLNKQQHYLLAGNCPAGYRSGSIGMNIQNSGSQFDCSSIQMAITNALNDWYFPETAGNFSYTYSCSSTSFVVNYKNIPAGYRPFIDALVQVNNGVSVVMNYVNTYTCTGSRSSINNGGTTTWGAYENNDAGGNGEAVEVVTQTYTGSTTKISTMPFSTSSDTTKTIVVNVPIPTVTTTTTYVGISTSYTTKTALPGSTANVIEYNPVHTTTTVTTCSPGQVSTITTTYSTSTWATDTVFIVTECPSPSSTQETPSTGEISST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJZ5</accession>
    <name>G3AJZ5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_134248</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373630.1">
        <property type="nucleotide sequence ID" value="XM_007373568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJZ5"/>
    <dbReference type="STRING" id="619300.G3AJZ5"/>
    <dbReference type="GeneID" id="18869796"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134248"/>
    <dbReference type="eggNOG" id="ENOG502QUEK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021797_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJZ5"/>
    <dbReference type="OMA" id="WEYLFSQ"/>
    <dbReference type="OrthoDB" id="2595934at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051089">
        <property type="entry name" value="prtT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR OF PROTEASES PRTT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="29"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="102"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="102"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="585" mass="66884" checksum="2D3CB61B9DDC3E9E" modified="2011-11-16" version="1">MNNSNGTKKRKHTPSETRKLAQASRILKACDNCRKQKTRCFRSPENPNSCVRCHALNKSCSFETFNFDPYFTDNNKLDLIYNGINEVLGLLKNTPTMVATPISNSSSINSQQQENEKEDSNEFVDDSTQFQSPPSSFITSPFLIISNSVPESVPSPIHKLLNPSKYHSEPNDVISLSILSESDAITLMDEFRRDFGKWISLPIYISTKDWIDEIRIKSSLLLTTGCCITIGKTTSSLPVELSNKLIQDMNECILKYSFYHRKDGIIEFLQAMVLLSTYSISISSLVLDSEANLDSWTLSQIGLSTFISSSTFGHLKATNDDITLTQLRIYNHLCLTHLFYCMISGRMCIIDEIRINQCYGTLSLLNATNFDGRMVAEINMILITYTFIQLNNNVQDLSELDHNLMNVLEETKLWYQQWEYLFSQPTIQFVELNYHFCTILIYYGYEYHKSKITGRKVEFLSNASLNVLNYANEFSLIEMFNHAYSLVKEINNLEDDIGFSYLSDQIHFCFYFAALCLIKILYQCTTGKGYSEQLSEKENVMEVVNTLVDKFSTISRGDSNNAAFKYKSGIEQILQSHFPEYSDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK77</accession>
    <name>G3AK77_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L-serine ammonia-lyase</fullName>
            <ecNumber evidence="5">4.3.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_50409</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-serine = pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:19169"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="EC" id="4.3.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.17" evidence="5"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375053.1">
        <property type="nucleotide sequence ID" value="XM_007374991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK77"/>
    <dbReference type="FunCoup" id="G3AK77">
        <property type="interactions" value="502"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK77"/>
    <dbReference type="GeneID" id="18871440"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50409"/>
    <dbReference type="eggNOG" id="KOG1250">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021152_3_1_1"/>
    <dbReference type="InParanoid" id="G3AK77"/>
    <dbReference type="OMA" id="AEQGCEH"/>
    <dbReference type="OrthoDB" id="6021743at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06448">
        <property type="entry name" value="L-Ser-dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000040">
        <property type="entry name" value="L-serine dehydratase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF2">
        <property type="entry name" value="CATABOLIC L-SERINE_THREONINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="11">
        <location>
            <begin position="25"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW33538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="348" mass="38629" checksum="E7F14EB687838EF0" modified="2011-11-16" version="1">METLDLQPTALRSPHVKTSLIEVTHKLPTRPPCRVFFKNELEQPSGSFKLRGIGNLVRTSIEKAHREHPNKTIHVFASSGGNAGLAAGYSAYFYKVKCTVVLPVISKKSVQDKLRSYGAEIVLFGNTINEADQHLKNLLNSIDLEQIYPIYCHPFNNPLIWEGHSTLVDEVTSQLGTKNIKGMVCSFGGGGLYNGIYQGMERNNINGEMLLIETLQAPTLTETLQADKIITLSSVKSLATSLACSYTTEKSLEYFKNKSQIHSKLETIDDIDSLRACVAYNKNCKKVVEPACGAALTVAYNRMDLLYKSFSNLKQDDVVVIVVCGGSCTTDKDLDEFQRIIRRSEIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKA0</accession>
    <name>G3AKA0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Cysteine proteinase 1, mitochondrial</fullName>
            <ecNumber evidence="2 9">3.4.22.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_137654</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Has aminopeptidase activity, shortening substrate peptides sequentially by 1 amino acid. Has bleomycin hydrolase activity, which can protect the cell from the toxic effects of bleomycin. Has homocysteine-thiolactonase activity, protecting the cell against homocysteine toxicity.</text>
    </comment>
    <comment type="function">
        <text evidence="7">The normal physiological role of the enzyme is unknown, but it is not essential for the viability of yeast cells. Has aminopeptidase activity, shortening substrate peptides sequentially by 1 amino acid. Has bleomycin hydrolase activity, which can protect the cell from the toxic effects of bleomycin. Has homocysteine-thiolactonase activity, protecting the cell against homocysteine toxicity. Acts as a repressor in the GAL4 regulatory system, but this does not require either the peptidase or nucleic acid-binding activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.</text>
            <dbReference type="EC" id="3.4.22.40"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homohexamer. Binds to nucleic acids. Binds single-stranded DNA and RNA with higher affinity than double-stranded DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.40" evidence="2 9"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375074.1">
        <property type="nucleotide sequence ID" value="XM_007375012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKA0"/>
    <dbReference type="FunCoup" id="G3AKA0">
        <property type="interactions" value="718"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKA0"/>
    <dbReference type="GeneID" id="18870064"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137654"/>
    <dbReference type="eggNOG" id="KOG4128">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038600_0_1_1"/>
    <dbReference type="InParanoid" id="G3AKA0"/>
    <dbReference type="OMA" id="QSYTFFW"/>
    <dbReference type="OrthoDB" id="2666448at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070005">
        <property type="term" value="F:cysteine-type aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043418">
        <property type="term" value="P:homocysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004134">
        <property type="entry name" value="Peptidase_C1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363:SF2">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03051">
        <property type="entry name" value="Peptidase_C1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005700">
        <property type="entry name" value="PepC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0788">Thiol protease</keyword>
    <feature type="active site" evidence="10">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="377"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR005700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005700-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="448" mass="51474" checksum="90F86BA7A158A0AC" modified="2011-11-16" version="1">MPARLDNLTNYTVNIENVIDNDLLNNFKDDFMQSVKNNLTQNAFSRNSIQDVITRDDINTKLRDQFIFNIDIDMPNSPYNNQGGSERCWVFAGCNVLRNEVIRKYNLTTDFQLSQSYVWFYDQLEKANLFLEKMVILADEPFDEVESDLQKPVDGGGFWDNFAALVLKYGLVPHDVFPDTYQAIDPSTLVNVLTSKLQEFGLKIRQLGQENPDQVDDYKEECLQVIYNILALTLGTPPKPEEEFTWEFANTDGEVTSFNTTALDFYEDHVDYNVTDFFVIMNDPTKEYNKSYVASASGMVNHTAAIGVNVEIKKMKDIAIDMLKDGKAVYFAAKYDFSVSETKGVFDTEIFDFYSAFDFELNLDKKERKLLSIGANHAMVIAGVHIDPNTNEPVRWKVQNSYGTEKGHNGWFLMTDDWFDEAVDQIVSSKDYVSDEIYDIWTSGEYYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKC8</accession>
    <name>G3AKC8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA repair protein RAD5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_71410</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375102.1">
        <property type="nucleotide sequence ID" value="XM_007375040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKC8"/>
    <dbReference type="FunCoup" id="G3AKC8">
        <property type="interactions" value="1033"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKC8"/>
    <dbReference type="GeneID" id="18875378"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_71410"/>
    <dbReference type="eggNOG" id="KOG1001">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_2_5_1"/>
    <dbReference type="InParanoid" id="G3AKC8"/>
    <dbReference type="OMA" id="KVEPWSN"/>
    <dbReference type="OrthoDB" id="2801544at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014905">
        <property type="entry name" value="HIRAN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF22">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08797">
        <property type="entry name" value="HIRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00910">
        <property type="entry name" value="HIRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="499"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="868"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="963"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="19"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="108"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="338"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="339"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="428"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW33587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1135" mass="130114" checksum="DAB603E434B35683" modified="2011-11-16" version="1">MSVTKKRFFVPKEKVTVPSLDDKSSVESTALSPNTSLFVEDDDDEDEADNESDVTPLPIQIPLGQFETELKATVGDISPQILSYLYNKHSNQSDGVKQAVSELLTCPPDINELLPNSTQQTESPAPPTQKRPTDDVNEQLQSLSKRIHLASQAHQKEVAQQTWDRYIGSIDVQAWATRPTFSPLKYAEKLSLKRLIPKNSSMKSSSIIRLSTIDHYGGREIARIPEDLTRIFSPLIDLDIARFEVSVLEGTKRRLSTADSFLIQIDVFLKNTGFIKNLDALEVKIDNLSKHKNAASKAAKSSNFHATSETDGEAALRLRQYALSRLFDRLRIKPLRLNDDDEEEENSDEPISLDSDDENEQVESTPDQLNLDQLKQFYQANNQSKLLESLPETTVPPSDNFKLNLRTYQKHGLSWMLTRENEISTLDQLSSEQGLSTQSKRDIEENESGTMNPLWRKYKWPKNMSFAVSVGGSTQSSQEREDEFFYANVYNGELSVEKPIIKNSLRGGILADEMGLGKTISALALVNSVPYDTNPEKSNKPYASKTTLIVVPMSLLSQWKQEFEKCNNNNNHYCKLYYGDEIESNLTWSLCSTKPNAKIPTVMITTYGTVLNEFTRIARARDEKGELPPIGLYSVKFFRIIIDEGHNIRNRNTKTAKSLYELESSRKWILTGTPIVNRLDDLYSFTKFLQLDPWSNFSYWKTFVTLPFEQRKISQTLDVIKSILEPIFLRRTKAMKGRDGRPLVELPSKEVIIEEIKFNDQEEKLYGYFKARAFNSFAEGLKSGQLLRQYTQILTHILRLRQVCCHVDLIGGAHEMDDEIIDLESDEDMKKFLKSIKEQQQNRFENDHAVKKTMYSLYSKVDIENSECSICTQSPIPFGEMTITPCGHSYCLTCLLEHLDFPTTTKTCPNCREPISKYQLFRLRNQKTTANEIRFHTKEPKAENYPFQLYLYDPNRSSSKIQALIKHLHDIKSQTPNSKVIVFSQFSSYLDIIETELKVQQDNDFVIYKFDGRLNLKERQKLLDDFNKELSDGKIAILLLSLKAGGVGLNLTTASRAFMMDPWWSPSIEDQAIDRIHRIGQNETVKVVRFIMENSIETKMLKIQERKKQIGEAVAAEEEERRKRRIEEIQILFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKI5</accession>
    <name>G3AKI5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Replication protein A subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_135958</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">As part of the replication protein A (RPA/RP-A), a single-stranded DNA-binding heterotrimeric complex, may play an essential role in DNA replication, recombination and repair. Binds and stabilizes single-stranded DNA intermediates, preventing complementary DNA reannealing and recruiting different proteins involved in DNA metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the heterotrimeric canonical replication protein A complex (RPA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the replication factor A protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375105.1">
        <property type="nucleotide sequence ID" value="XM_007375043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKI5"/>
    <dbReference type="FunCoup" id="G3AKI5">
        <property type="interactions" value="1171"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKI5"/>
    <dbReference type="GeneID" id="18869930"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135958"/>
    <dbReference type="eggNOG" id="KOG0851">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012393_2_0_1"/>
    <dbReference type="InParanoid" id="G3AKI5"/>
    <dbReference type="OMA" id="DQCDAFY"/>
    <dbReference type="OrthoDB" id="1751331at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045184">
        <property type="term" value="P:establishment of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030491">
        <property type="term" value="P:heteroduplex formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043934">
        <property type="term" value="P:sporulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04474">
        <property type="entry name" value="RPA1_DBD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04475">
        <property type="entry name" value="RPA1_DBD_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04476">
        <property type="entry name" value="RPA1_DBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000041">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000064">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000090">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047192">
        <property type="entry name" value="Euk_RPA1_DBD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007199">
        <property type="entry name" value="Rep_factor-A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031657">
        <property type="entry name" value="REPA_OB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004591">
        <property type="entry name" value="Rfa1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00617">
        <property type="entry name" value="rpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04057">
        <property type="entry name" value="Rep-A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16900">
        <property type="entry name" value="REPA_OB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Replication factor-A protein 1 N-terminal" evidence="12">
        <location>
            <begin position="6"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="OB" evidence="11">
        <location>
            <begin position="193"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A OB" evidence="14">
        <location>
            <begin position="301"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Replication factor A C-terminal" evidence="13">
        <location>
            <begin position="457"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="138"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="160"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW33590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="626" mass="70642" checksum="3FB007BF1F077A82" modified="2011-11-16" version="1">MTLPPLTTNALKDIFSTTAKDTVKTPLILQVTNIKHVEGGSRSFRVLLFDGVFSGQGLVEESCGTYLKNNGFARYQYIQVNDFSTVITQRHIIILKNVEILGQGEKTENQAKQIEAYYKEHPEEDFLALQKAKGETYPNADVAKTRSESPFTPQTTTSTPAPPAPKPAPPVSASGAPIRITPIETISPYQNNWTIKARVSYKGDLRTWSNAKGTGQLFSVNFLDESDEIKATAFNETAERAYNLLEEGKVYYISKARVAAAKKKFNHLTHPYEITMEKDTEITECFDNTNVPKLHFNFVKLNKIQDLESNAIVDVIGALKIVNEPFKITAKSTGKEFDRRNITIVDETGFAIEMGLWNNTAVEFNIEQGSIIAFKGCKVQDYNGRSLTLTQQGSVIPNPESPEAYQLKGWYDNQGINESFKSLKVESSGASSNQIENRKSIAQAQDENLGKGEKPDYFTVKATISYTKPDTFAYPACPNVVASNAGSQQARPSQPCNRKLVEQPTDGTWRCERCDINYPEPTYRYIYNCSILDETGQIWVTLFDNEARKLFGIDAGELTKIKEEDQDEFTRRIEDISFKEYQFRLRARQDTYNDQLRVRYQAVGIDFIDYSTEAEYLCKQLDNLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKL7</accession>
    <name>G3AKL7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chromatin structure-remodeling complex protein RSC7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_55466</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375137.1">
        <property type="nucleotide sequence ID" value="XM_007375075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKL7"/>
    <dbReference type="FunCoup" id="G3AKL7">
        <property type="interactions" value="323"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKL7"/>
    <dbReference type="GeneID" id="18871940"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55466"/>
    <dbReference type="eggNOG" id="ENOG502QW07">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022149_1_2_1"/>
    <dbReference type="InParanoid" id="G3AKL7"/>
    <dbReference type="OMA" id="STNWLYQ"/>
    <dbReference type="OrthoDB" id="5598844at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013933">
        <property type="entry name" value="CRC_Rsc7/Swp82"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22597:SF3">
        <property type="entry name" value="CHROMATIN STRUCTURE-REMODELING COMPLEX SUBUNIT RSC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22597">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08624">
        <property type="entry name" value="CRC_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="26"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="76"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="108"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="442" mass="50540" checksum="84CD6A7F456FA4D9" modified="2011-11-16" version="1">MAKRGRRGEIKQEPEVDELEDVKVDEVEDDVNDEYVEEEEEAAEEDEEEEEEDEDVDEDSIIKRRSRKRKSSSRDVDDDEEDEPEDEENQDEEEEEEEEEATQEPEDGEVKQEPAEGEEVKTGPKKRGRKRTKFTVLEEGVFDADGNPISVIDDEVVINHEDPKGKEKIDEFGNLQGGREFRMKTFKLLGKGDKLYMISTEPARLVGFRDSYLLFKTHRTLFKKVCTHEEKMDLINRGLIPNSYKGRSVNLVAARSIFREFGARMIKQGKKVIDDFWEQRAIDAGDIAGDYADPNELFPNQSKLSTIFGDGVSVGSGGGATPLTATPIVNYQSDPSWMYQIAKQTQEYNHKLMEHRSQLMNKGVKDIYSNLTFYPESSQPTHYKVYKYDGETEPGLKYNVKMVGSVKPVTGLSKLSKELLAEIDDEEIKQAIIDQQQYEASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKN6</accession>
    <name>G3AKN6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein-sorting-associated protein 24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60973</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375156.1">
        <property type="nucleotide sequence ID" value="XM_007375094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKN6"/>
    <dbReference type="FunCoup" id="G3AKN6">
        <property type="interactions" value="622"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKN6"/>
    <dbReference type="GeneID" id="18873664"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60973"/>
    <dbReference type="eggNOG" id="KOG3229">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069208_0_2_1"/>
    <dbReference type="InParanoid" id="G3AKN6"/>
    <dbReference type="OMA" id="KILWEVT"/>
    <dbReference type="OrthoDB" id="2329734at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="25522" checksum="D5F974C645390E88" modified="2011-11-16" version="1">MDFIKKQIYGPDPKDQMRKINQLLRKNKRELDRSLNQLQPLKKKTESLIKKAAKEKDYKTAKLYAKELIGINRQYNKLYTSRTRIESINMTINEQWQMNKLTQSIGASTSVMKDVNRLIHVGAVSQTMQELSKELMKAGIINEMVDDMVDVDFEEDEELESESQEEINKIITSLTEDKFSKISHEVPTTKLEEPAEEEAPEEEDEIALDEMRQRLKALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKU4</accession>
    <name>G3AKU4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="5">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_55482</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="5"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375160.1">
        <property type="nucleotide sequence ID" value="XM_007375098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKU4"/>
    <dbReference type="FunCoup" id="G3AKU4">
        <property type="interactions" value="409"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKU4"/>
    <dbReference type="GeneID" id="18871941"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55482"/>
    <dbReference type="eggNOG" id="KOG1864">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008279_12_1_1"/>
    <dbReference type="InParanoid" id="G3AKU4"/>
    <dbReference type="OMA" id="PMHGHYV"/>
    <dbReference type="OrthoDB" id="27652at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02663">
        <property type="entry name" value="Peptidase_C19G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000131">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF733">
        <property type="entry name" value="RE52890P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="7">
        <location>
            <begin position="24"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="89"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="582"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="195"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="619"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="684" mass="77175" checksum="08FF3D3CE16C7F17" modified="2011-11-16" version="1">MTKPYPEPNPPVILPYGDGSYKIFGMENFGNTCYCNSILQCLYYTENFRKNLVSHKFTKHEPKLKISGSKLHSFSNKYEQLLQKKLKEQGKVPTQTPISAGSVVSGSGGLEERPKSSRKGSLFGIKFNNSSSTTTAANVIDSKTASYIREAKDWDVLTLEQRLNIKKCAEFQKLPILMTRPTNHEVNPSGRVDYSQSSSMLLNGDQPQQTQSEGSISSTSSAIIVGIPHPETNLLVPINPFNVNPGADQRKRSALINGPIINLDASLQIGEQHEDSILMYALKDIFESMIENKSTIGVVSPNYFIQKLKDRNYLFRQNNMHHDAHEFFNYLINEIIETLNREVGPENNWCNNIFRGSITNETKCLSCETVSSRHEYFLDLSIDIPPGESAYSLSYSLNNFSKSETLTNQNKFYCNTCSSLQEAVKTIKLNKLPEVLVINFKRFKYDETVDKMVKLFDSISYPFKLKLFNTTDDDQEQHLYELYALVVHIGGGPMHGHYVSICKTKYGLWLLFDDETVELVDDSYVMRFFGNGPGLASAYILFYSKCHETSDDVVDFGFDISSIYKGDDFGLLQQVNNNSDLSLKKPTTAEEVEQEKSETSSVNSYNAPEAKKQGVFKPFKFDGTKEEKPLSRSSSNKEPKEKKSWGLKWKNSSDTTNSDKKASVSGASSSVPERKKSIFGFKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKU6</accession>
    <name>G3AKU6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_136981</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BLOC1S2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375164.1">
        <property type="nucleotide sequence ID" value="XM_007375102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKU6"/>
    <dbReference type="GeneID" id="18870008"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136981"/>
    <dbReference type="eggNOG" id="ENOG502SG83">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2184929_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKU6"/>
    <dbReference type="OMA" id="DEEYGAY"/>
    <dbReference type="OrthoDB" id="445556at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019269">
        <property type="entry name" value="BLOC1_su2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10046">
        <property type="entry name" value="BLOC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="40"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="114" mass="12843" checksum="8F601F43B7C8F370" modified="2011-11-16" version="1">MSTSRDTPKLVSLALNAASKIVESDTNVSTIDLNLLENLNTNQSLNYIKLEKNLDDMETNSNQLKQLKAEFESHSQELISIENKIAILEEITTELDQWSKQLEHNVESAVNKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKY8</accession>
    <name>G3AKY8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Deoxyhypusine hydroxylase</fullName>
            <shortName evidence="10">DOHH</shortName>
            <ecNumber evidence="10">1.14.99.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Deoxyhypusine dioxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Deoxyhypusine monooxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LIA1</name>
        <name evidence="12" type="ORF">SPAPADRAFT_150589</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate produced by deoxyhypusine synthase/DHPS on a critical lysine of the eukaryotic translation initiation factor 5A/eIF-5A. This is the second step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine. Hypusination is unique to mature eIF-5A factor and is essential for its function.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[eIF5A protein]-deoxyhypusine + AH2 + O2 = [eIF5A protein]-hypusine + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:14101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="Rhea" id="RHEA-COMP:12592"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="ChEBI" id="CHEBI:91175"/>
            <dbReference type="EC" id="1.14.99.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="10">Binds 2 Fe(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the deoxyhypusine hydroxylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.29" evidence="10"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374546.1">
        <property type="nucleotide sequence ID" value="XM_007374484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKY8"/>
    <dbReference type="FunCoup" id="G3AKY8">
        <property type="interactions" value="915"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKY8"/>
    <dbReference type="GeneID" id="18870871"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_150589"/>
    <dbReference type="eggNOG" id="KOG0567">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053974_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKY8"/>
    <dbReference type="OMA" id="LQEPCSI"/>
    <dbReference type="OrthoDB" id="421002at2759"/>
    <dbReference type="UniPathway" id="UPA00354"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019135">
        <property type="term" value="F:deoxyhypusine monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000099">
        <property type="entry name" value="Deoxyhypusine hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000292">
        <property type="entry name" value="Deoxyhypusine hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03101">
        <property type="entry name" value="Deoxyhypusine_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027517">
        <property type="entry name" value="Deoxyhypusine_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697:SF5">
        <property type="entry name" value="DEOXYHYPUSINE HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697">
        <property type="entry name" value="PBS LYASE HEAT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="11">
        <location>
            <begin position="86"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="11">
        <location>
            <begin position="245"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW33031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="318" mass="35597" checksum="20E4EB625BD06D49" modified="2011-11-16" version="1">MTADINIDTANLEQLRDILVNKSGDVKLANRFRALFNLKSIGADQTEQPEQARKAVEYISECFKDSSELLKHEVAYVLGQTKNLTAAPYLRDVLIDDNQQVMVRHEAAEALGALGDSESLPLLKKYFVEDPALEIRQTCELAIERINWENSESAKTEKLEKSLYTSIDPAPPLATDQSSSKIEKLQQILNDQDKPLFERYRAMFRLRDLGTDEACLALASGFDDPSALFKHEIAYVFGQLCNPVTVPSLIKVLKDESQAGMVRHEAAEALGSIATDECLPVLQSFLEDKEDVVRDSAIVALDMYEYENSNELEYATVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALF4</accession>
    <name>G3ALF4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_66191</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374712.1">
        <property type="nucleotide sequence ID" value="XM_007374650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALF4"/>
    <dbReference type="STRING" id="619300.G3ALF4"/>
    <dbReference type="GeneID" id="18875087"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66191"/>
    <dbReference type="eggNOG" id="ENOG502S831">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037138_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALF4"/>
    <dbReference type="OMA" id="NTWELMS"/>
    <dbReference type="OrthoDB" id="4026335at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="491" mass="55861" checksum="2A3F879F5DA9ADCD" modified="2011-11-16" version="1">MFTKSVNDRALYERLTGIQEFSGLPASSLGLTSGQVLNALDTSREEYSTLDLFGVHVSRVLLTILLFVYMRMFGIRSYLSNAWMFLKVFWFDLQLNSLNSRPQDSTNPFVLIANAIVFYWGILLNVPAELLRVSKQVFINTPIGLDNCKRYIPKANTPSSVTLILDLTPHVLLPPPEIPQPYLDADRNIQVPDKKEVQFVIAVRNEYFTQYKSHIAAERVRLLRDISRFITWCCLVPSINELTIYEKQGIVWDAEDYNGIEAMKNSILVELESFASSCKKHELQEFKACIPSITLVDWFNKTRFSVNEPDDIVGNDQHIEIEDVLSSYEEQRQLVVNLCDLRIRETNFKKLTSKILQESLESSDNVFNEELPSTPDLIVAPCNSGRVNNSLLGYACIDQSIDDKTKSMVIFSDLEFGFPFFSKSLYNYALDQPIESPKGTQVPSNTVLRMAQSRNIAHYVKKVKNLEFQPSVDVRSPVSPRQVASPLAVNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALR1</accession>
    <name>G3ALR1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60646</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374819.1">
        <property type="nucleotide sequence ID" value="XM_007374757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALR1"/>
    <dbReference type="FunCoup" id="G3ALR1">
        <property type="interactions" value="233"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALR1"/>
    <dbReference type="GeneID" id="18873502"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60646"/>
    <dbReference type="eggNOG" id="KOG1411">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032440_1_2_1"/>
    <dbReference type="InParanoid" id="G3ALR1"/>
    <dbReference type="OMA" id="VGACTIV"/>
    <dbReference type="OrthoDB" id="6752799at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006533">
        <property type="term" value="P:L-aspartate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000066">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF22">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="53"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="435" mass="48848" checksum="5582FE91E87AD5EB" modified="2011-11-16" version="1">MYRTQLAKQVSTKRILLKQFSTTSQLSSWNDIPLAPPDKILGITEAFKEDKNTKKINLGVGAYRDNQGKPIIFPSVKQAETKLLQTETEKEYTPINGSKTFQNIVKNFIFNNSNKDANGKQLIEDGRIVTAQTISGTGSLRVIADFLNRFYSSKKILVPQPTWANHVAVFADAGLTPEYYAYYDKSKNDLDYANLKKSLAEQPEGSIVLLHACCHNPTGMDLTPEQQDEVLQIVQDKKFFPLVDMAYQGFGSGIPYEDIGFIRKLTALANSNKIPSFALCQSFAKNMGLYGERTGSISIITSSGEASKAVESQLKKLIRPMYSSPPIHGSKIVETIFQDEALLQSWLSDLNQVVGRLNTIRQTLYDRLDKTNYNWDHLLKQKGMFVYTGLSSEQVIRLRNEFSVYATEDGRFSISGINDNNVEYLANAINEVIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM91</accession>
    <name>G3AM91_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">V-type proton ATPase subunit F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_60752</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1).</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Vacuole membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the V-ATPase F subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374904.1">
        <property type="nucleotide sequence ID" value="XM_007374842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM91"/>
    <dbReference type="FunCoup" id="G3AM91">
        <property type="interactions" value="728"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM91"/>
    <dbReference type="GeneID" id="18873546"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60752"/>
    <dbReference type="eggNOG" id="KOG3432">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135754_0_0_1"/>
    <dbReference type="InParanoid" id="G3AM91"/>
    <dbReference type="OMA" id="IIICQHI"/>
    <dbReference type="OrthoDB" id="10261947at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10580:FF:000002">
        <property type="entry name" value="V-type proton ATPase subunit F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10580">
        <property type="entry name" value="ATPase, V1 complex, subunit F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008218">
        <property type="entry name" value="ATPase_V1-cplx_f_g_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005772">
        <property type="entry name" value="ATPase_V1-cplx_fsu_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036906">
        <property type="entry name" value="ATPase_V1_fsu_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01101">
        <property type="entry name" value="V_ATP_synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13861:SF2">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13861">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01990">
        <property type="entry name" value="ATP-synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015945">
        <property type="entry name" value="ATPase_V1_F_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159468">
        <property type="entry name" value="AtpF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR015945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW33389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="125" mass="13769" checksum="5E37745426FAD060" modified="2011-11-16" version="1">MSSASESMAKRKLLATIADEDSVTGLLLAGVGQISNEVGKESNFLTVIPGKTTVDQIEDAFDNFTTGRDDIAILLINQHLADLIRYKVDSYTNAFPAILEIPSKDHPYDPEKDSILKKVRRLFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMC4</accession>
    <name>G3AMC4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein ALD5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ALD5</name>
        <name evidence="7" type="ORF">SPAPADRAFT_136378</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374937.1">
        <property type="nucleotide sequence ID" value="XM_007374875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMC4"/>
    <dbReference type="FunCoup" id="G3AMC4">
        <property type="interactions" value="927"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMC4"/>
    <dbReference type="GeneID" id="18869962"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136378"/>
    <dbReference type="eggNOG" id="KOG2450">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_0_1_1"/>
    <dbReference type="InParanoid" id="G3AMC4"/>
    <dbReference type="OMA" id="WSNTFNK"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019413">
        <property type="term" value="P:acetate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07091">
        <property type="entry name" value="ALDH_F1-2_Ald2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000011">
        <property type="entry name" value="ALD5p Mitochondrial aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000001">
        <property type="entry name" value="Mitochondrial aldehyde dehydrogenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="6">
        <location>
            <begin position="35"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="501" mass="53903" checksum="D25194D2374DD9FB" modified="2011-11-16" version="1">MSKSQLPLTVKLTLPTGVTYEQPVGLFINNEFVYPKQKKTFEVVSPSTEEVITEVYEALQEDVETAVVAAEEAYKSSWSTGPPTVRMSVLLKLADLIEADAEIIAAIESYDNGKSLQNARGDVALTAAYFRSCAGWADKFMGTVIETGDTHFNYTRREPIGVCGQIIPWNFPLLMASWKLGPVIATGCTTVLKTAESTPLSALYLATLLVKAGLPKGAVNIISGFGPTAGSAIASHPRINKVAFTGSTATGRIIMKAAADSNLKKVTLELGGKSPNIVFNDADLDQTIGHLITAIFYNSGEVCCAGSRVLIQSGIYDEVVKKFKVAAESVKTGNPFEESTFMGAQASEIQLSKILKYIELGKKQGATVVTGGERCAGKGYFVKPTIFADVHRDMDIVKDEIFGPVVTLIKFDTVDEAVALANDTEYGLAAGIHSTDVNKVLSVSSRIKAGTIWVNTYNDFHPMVPFGGYGASGIGREMGEEVFHEYTQVKAVRLHINKPTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMD4</accession>
    <name>G3AMD4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_60134</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for the assembly of the V0 complex of the vacuolar ATPase (V-ATPase) in the endoplasmic reticulum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasmic vesicle</location>
            <location evidence="6">COPII-coated vesicle membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the VMA21 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374301.1">
        <property type="nucleotide sequence ID" value="XM_007374239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMD4"/>
    <dbReference type="FunCoup" id="G3AMD4">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMD4"/>
    <dbReference type="GeneID" id="18873232"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60134"/>
    <dbReference type="eggNOG" id="ENOG502SBNA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154717_1_0_1"/>
    <dbReference type="InParanoid" id="G3AMD4"/>
    <dbReference type="OMA" id="VMAFMED"/>
    <dbReference type="OrthoDB" id="160405at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03058">
        <property type="entry name" value="VMA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019013">
        <property type="entry name" value="Vma21"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09446">
        <property type="entry name" value="VMA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="2 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Prevents secretion from ER" evidence="6">
        <location>
            <begin position="75"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="78" mass="8568" checksum="937C99C2B7B0EBE1" modified="2011-11-16" version="1">MSAPDIPKSVIQKLVFFTGAMIILPVFTFFVCQYLFNHNSIISGGIAALIANVVLIGYVVVAFTEDTSKLEEESKKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMD6</accession>
    <name>G3AMD6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein BIG1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_49735</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BIG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374304.1">
        <property type="nucleotide sequence ID" value="XM_007374242.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMD6"/>
    <dbReference type="STRING" id="619300.G3AMD6"/>
    <dbReference type="GeneID" id="18871367"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49735"/>
    <dbReference type="eggNOG" id="ENOG502S5C0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082455_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMD6"/>
    <dbReference type="OMA" id="KYQFFTS"/>
    <dbReference type="OrthoDB" id="10262235at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037654">
        <property type="entry name" value="Big1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046756">
        <property type="entry name" value="VAS1/VOA1_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28285">
        <property type="entry name" value="PROTEIN BIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28285:SF1">
        <property type="entry name" value="PROTEIN BIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20520">
        <property type="entry name" value="Ac45-VOA1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442504" description="Protein BIG1" evidence="11">
        <location>
            <begin position="19"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="V-type proton ATPase subunit S1/VOA1 transmembrane" evidence="12">
        <location>
            <begin position="230"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW32789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="278" mass="31487" checksum="E3DC9DAB2647C67D" modified="2011-11-16" version="1">MKVQSLSLIASVLSVVNAFADTALLYSSFDLEVPHKYITESKDLSQFMLQYIQQLCANDKQLYIYRVNQLKSSQVDHGVLLNHVHYNSISDLSFPIDAACSLEIVSEQDSNQSQSNIVVVEVSEGVHSIDEFLKHENVIVQGVPSFHKRNKVNQIVEQVNDMFHDDVEKRDVADGSNYDDIQSEVEADFREAESLIDDETVTVFKASIKEARASNDTKKSTNTSLFTNYQFFTPGIWSGIIVSGFLLTILYLGINWLNSLEVTYSSFEKQVDYDKKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMS6</accession>
    <name>G3AMS6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Adenine phosphoribosyltransferase</fullName>
            <ecNumber evidence="8">2.4.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_60865</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:16609"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.7" evidence="8"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375035.1">
        <property type="nucleotide sequence ID" value="XM_007374973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMS6"/>
    <dbReference type="FunCoup" id="G3AMS6">
        <property type="interactions" value="571"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMS6"/>
    <dbReference type="GeneID" id="18873606"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60865"/>
    <dbReference type="eggNOG" id="KOG1712">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063339_1_0_1"/>
    <dbReference type="InParanoid" id="G3AMS6"/>
    <dbReference type="OMA" id="QAYDLEY"/>
    <dbReference type="OrthoDB" id="363185at2759"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00646"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002055">
        <property type="term" value="F:adenine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003999">
        <property type="term" value="F:adenine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006168">
        <property type="term" value="P:adenine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000004">
        <property type="entry name" value="Adenine phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00004">
        <property type="entry name" value="Aden_phosphoribosyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005764">
        <property type="entry name" value="Ade_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050054">
        <property type="entry name" value="UPRTase/APRTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01090">
        <property type="entry name" value="apt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002636">
        <property type="entry name" value="PRK02304.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315:SF3">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="14">
        <location>
            <begin position="43"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00011893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00017366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW33520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="188" mass="20638" checksum="BDCAB552AF4F369B" modified="2011-11-16" version="1">MSSTHNEIVELAAELKANLKQFPNFPQEGILFEDFLPIFTKPDLFKKLVRAFQLHVGEDAKVDYVIGLESRGFLFGPALALALNAGFVPVRKPGKLPGPTYNVEFKKEYGSDAFEIQKDVIPAGSRVLIVDDILATGGSAFGAGELAKLTGAKIVEYLFVMELDFLKGRDRLDAPVFTLFGGQTEKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMV3</accession>
    <name>G3AMV3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_139015</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375643.1">
        <property type="nucleotide sequence ID" value="XM_007375581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMV3"/>
    <dbReference type="STRING" id="619300.G3AMV3"/>
    <dbReference type="GeneID" id="18870172"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139015"/>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005403_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMV3"/>
    <dbReference type="OMA" id="PLHFSGE"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="136"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="172"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="417"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="598"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="82"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="417"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="434"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="467"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="508"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW32367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="860" mass="97908" checksum="13A337D697979382" modified="2011-11-16" version="1">MDIKENSDDDQADSDNELSEAETVISTSSPRTNKVRRLIRKKDYDEERHKLKRAGVDANNYQSDEEEDEEEEEEEEEEPKSKRSSVSRGSKSRKPYKIKRDAGGRTLLQRACKKGNLEDVIDYISRGASANEKDFCGFTCLHEAALEGHADIVAYLIEHGANVNAKADEVGDCETPLIDAAENKHLETVKILLKHGADPTIFNLDGFTALTKIYNEHADEEGYDDTIKVLEEAVTEFNKKAQASVGKGTSPSTPKEIVEDPNDNYFADLIKKKGIYKYAAENAKEITANYFVSGNSLDSKPDILILAARNGHAELVDIILGLNPTPYDIDTESPCGVTALLASVGRGHLDVVESLLSKDADPLRTRRKDGFNALDIASRSVHFDPKEVELIAKYVEKRGGNALAVSVASRLGSRIGSAVASRASSRAPSVQVSSEDDEEEETIEKDLKGGEPQVQENAETKEKPKEEEYEQDEEEDDEFSDHSMQDIDEEEMEPITPKRSREIKRKRSIDEMHSSIKRVKSEGRLKTPEMFKPEEEAEEHSSSRNATISPPPQLQKIKTISLSPPPLTKEQEELKIKNAEEARIWQEKVEAKKRARREMFLKSEREKEQKRKEEEEKRIEEEKRIAQLKEEERNRLIKEAEKLSMEVQKQRVAMRRAKTIEHYPIGLRNLKQFNSNPTKTTIYKYSPLYVFCIDGEKYVVDLQISLLTSTLMNEIHKLVPPSSVIDLANKSKIWKLFFKFIGLDPKGEKSILELRREGHDQFQNLLLHFMKLDDIMPVLEQKYPLVYNSIWSESHSRTIEVLLDSLCAFDEIVDKKNMTLEDGELDVIVDAHSIDKFIPHHLRFRKDILRTVSASTRPLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMX2</accession>
    <name>G3AMX2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-dehydropantolactone reductase</fullName>
            <ecNumber evidence="6">1.1.1.358</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2-dehydropantolactone reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Ketopantoyl-lactone reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_61459</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(R)-pantolactone + NADP(+) = 2-dehydropantolactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16719"/>
            <dbReference type="ChEBI" id="CHEBI:18395"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.358"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>isatin + NADPH + H(+) = 3-hydroxyindolin-2-one + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:68608"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27539"/>
            <dbReference type="ChEBI" id="CHEBI:28536"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.358" evidence="6"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375661.1">
        <property type="nucleotide sequence ID" value="XM_007375599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMX2"/>
    <dbReference type="FunCoup" id="G3AMX2">
        <property type="interactions" value="492"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMX2"/>
    <dbReference type="GeneID" id="18873906"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61459"/>
    <dbReference type="eggNOG" id="KOG1577">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMX2"/>
    <dbReference type="OMA" id="WPPFLYD"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047011">
        <property type="term" value="F:2-dehydropantolactone reductase (A-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042180">
        <property type="term" value="P:ketone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF3">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="12">
        <location>
            <begin position="15"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="11">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00079693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00081322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW32385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="301" mass="33834" checksum="ABB94251D679DB76" modified="2011-11-16" version="1">MATLKLNNNAEIPVLGLGTWQSSPEEVYNAVLHALKLGYRHIDTAFAYGNEESIGKAIRDSGIPREQLFITTKLWCTFHKNPSDGLKESLTRLGLDQVDLYLMHWPVPLNPNGNDPKFPTLPDGNRDIVQGWNFIDTWREMQKLVGHGAKAIGVSNFDVYNLEKLLQASSTTVVPAANQVELHPHLPQHKLLAYCKEKGIVLEAYSPLGSTNSPLLTEPALVKIAEKYHVHTATVLISWAIWRGTVVLPKSVTPKRIESNFEVIKLEDEDGKIIDNLYKEIGVRRIISPNWYPEVVFHSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMX9</accession>
    <name>G3AMX9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bul1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61465</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375669.1">
        <property type="nucleotide sequence ID" value="XM_007375607.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMX9"/>
    <dbReference type="STRING" id="619300.G3AMX9"/>
    <dbReference type="GeneID" id="18873909"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61465"/>
    <dbReference type="HOGENOM" id="CLU_1954058_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMX9"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007519">
        <property type="entry name" value="Bul1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04425">
        <property type="entry name" value="Bul1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bul1 N-terminal" evidence="2">
        <location>
            <begin position="12"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="129" mass="14193" checksum="89438228312DE674" modified="2011-11-16" version="1" fragment="single">MVDLSESTKSGFHEYDKSVNKPSYDETVGSILPSYSMFTNTIRMNATVPENEERDDNPPPNYQDTPSITSDDHGTSLSSSSHAQLSLGASDSANTRSTTTEEDDDLISSLNNNFIIADENTHAWQETIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN11</accession>
    <name>G3AN11_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent DNA helicase II subunit 1</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">ATP-dependent DNA helicase II subunit Ku70</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_138036</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ku70 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375701.1">
        <property type="nucleotide sequence ID" value="XM_007375639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN11"/>
    <dbReference type="FunCoup" id="G3AN11">
        <property type="interactions" value="682"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN11"/>
    <dbReference type="GeneID" id="18870093"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138036"/>
    <dbReference type="eggNOG" id="KOG2327">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024202_0_0_1"/>
    <dbReference type="InParanoid" id="G3AN11"/>
    <dbReference type="OMA" id="FWANVKH"/>
    <dbReference type="OrthoDB" id="3249161at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043564">
        <property type="term" value="C:Ku70:Ku80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00788">
        <property type="entry name" value="KU70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006164">
        <property type="entry name" value="DNA_bd_Ku70/Ku80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006165">
        <property type="entry name" value="Ku70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047087">
        <property type="entry name" value="KU70_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005160">
        <property type="entry name" value="Ku_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005161">
        <property type="entry name" value="Ku_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604">
        <property type="entry name" value="KU AUTOANTIGEN DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604:SF2">
        <property type="entry name" value="X-RAY REPAIR CROSS-COMPLEMENTING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02735">
        <property type="entry name" value="Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03730">
        <property type="entry name" value="Ku_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03731">
        <property type="entry name" value="Ku_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003033">
        <property type="entry name" value="Ku70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00559">
        <property type="entry name" value="Ku78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SPOC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Ku" evidence="18">
        <location>
            <begin position="327"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW32425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="682" mass="79077" checksum="1270E435A6CD1A04" modified="2011-11-16" version="1">MESDTEEDDKQYQQYEIRDGILFLIEITPDLLKSDSNLGSHSQLYEILSSINDLMSELIITMKNTGIGIYFYNCSPSPVLKPMNQPPGFYKLFRLNVLNLQNMKRLNDIIQDSAINPVEGLFKYKRMEDENHLSVVLNKIIDEFSSKTEFNRKKLIWITNNDKPYTETTTKDNLWRIVNDFYNYKLFIDPMFLSSGGKRFDTELYKDIFLNTNFLKPTQSQHDNEPTSKDSPVKSLLSSQIRQSIFRIKEVRRIQFSCDLILSDDGSVGGNFGCSIKGYTLYSHERYKKDLLLYTKEESIKKVHSSSEIISDSGKINLPKDKAKSLQESKEAAGIRKGYEIGGGQDVIFLNKEQVDFINNYAFDHRLIEKDEDGDEDDDDDDEDKTYIATSKPPYLKLLGFRDIQNFNPVYSCGSPIFITSDITTSTGYSNSLETLASLYRSCMKLQQYAIVFGCIKRNSSPYLYALYPTQTMNSTKLVEGEEFPQGFLLIKLPWIEDVRSLPQDFIKEIHEEPDATLVDQFKSLFKKYELDSYDPKQFPNPSLNYFYKVLKYEILQMELLPGERNLSSNDVTIQRLMELKQKFDSDDSAQYILNQINSRLSELEERIPKRVISEDKPVKKAKVVELDESAVLVKWNQGKLNDFTMEQLKTFVKKYPQIKVGSRKSETIENISRFLESREHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN73</accession>
    <name>G3AN73_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Endonuclease</fullName>
            <ecNumber evidence="17">3.1.30.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">SPAPADRAFT_61019</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the DNA/RNA non-specific endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.30.-" evidence="17"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375192.1">
        <property type="nucleotide sequence ID" value="XM_007375130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN73"/>
    <dbReference type="FunCoup" id="G3AN73">
        <property type="interactions" value="246"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN73"/>
    <dbReference type="GeneID" id="18873684"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61019"/>
    <dbReference type="eggNOG" id="KOG3721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055174_0_2_1"/>
    <dbReference type="InParanoid" id="G3AN73"/>
    <dbReference type="OMA" id="YVMPNQV"/>
    <dbReference type="OrthoDB" id="5418055at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004529">
        <property type="term" value="F:DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00091">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.570.10:FF:000004">
        <property type="entry name" value="Nuclease 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.570.10">
        <property type="entry name" value="Extracellular Endonuclease, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018524">
        <property type="entry name" value="DNA/RNA_endonuclease_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044929">
        <property type="entry name" value="DNA/RNA_non-sp_Endonuclease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001604">
        <property type="entry name" value="Endo_G_ENPP1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020821">
        <property type="entry name" value="ENPP1-3/EXOG-like_nuc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040255">
        <property type="entry name" value="Non-specific_endonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13966:SF5">
        <property type="entry name" value="ENDONUCLEASE G, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13966">
        <property type="entry name" value="ENDONUCLEASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01223">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00892">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00477">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01070">
        <property type="entry name" value="NUCLEASE_NON_SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="10 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 17" id="KW-0540">Nuclease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA non-specific endonuclease/pyrophosphatase/phosphodiesterase" evidence="19">
        <location>
            <begin position="75"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="ENPP1-3/EXOG-like endonuclease/phosphodiesterase" evidence="18">
        <location>
            <begin position="76"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR640255-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR640255-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU366055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EGW31916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="322" mass="36540" checksum="C86F6DD199F243C1" modified="2011-11-16" version="1">MSKYLLNTFGIGSIGIASYFWGKSQVPTPPSTPTGDSNLPVPYSPPGKDVFDPSTINPQSFFKYGFPGPIHDLANRSEFVSCYDRQFKNPYWVVEHLTKESIARNGNSGDRKKSVFKEDESIPFKFRGRLRDYFRSGYDRGHLAPAADAKFNQDAMDETFYLTNMSPQVGDGFNRDYWSHFEDFARRLTKKYDDVRVMTGPLYLPKKGPDGKFRVEFEVIGSPPNIAVPTHFYKLIVGENRGQEKISCAAFVLPNEVIDNNTPLTQFQVPIEAVEQSSGLELLHKVPYFNKKDLCKEVKCEIIVREFPKQVNNVLALPPGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANJ3</accession>
    <name>G3ANJ3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein ATP19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ATP19</name>
        <name evidence="4" type="ORF">SPAPADRAFT_61584</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375798.1">
        <property type="nucleotide sequence ID" value="XM_007375736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANJ3"/>
    <dbReference type="FunCoup" id="G3ANJ3">
        <property type="interactions" value="83"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANJ3"/>
    <dbReference type="GeneID" id="18873964"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61584"/>
    <dbReference type="eggNOG" id="ENOG502S99W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172736_1_2_1"/>
    <dbReference type="InParanoid" id="G3ANJ3"/>
    <dbReference type="OMA" id="FQPHQLA"/>
    <dbReference type="OrthoDB" id="2094445at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021278">
        <property type="entry name" value="ATP19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28074">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT K, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28074:SF1">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT K, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11022">
        <property type="entry name" value="ATP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="69" mass="7458" checksum="836266F7DF856412" modified="2011-11-16" version="1">MGAAYTILGRTFQPHQLAIATLGAVVYLVAPKPWAAKEVSAGPAINASSPEEEKFVKEWLAKHTAEEKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANM3</accession>
    <name>G3ANM3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_61617</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375828.1">
        <property type="nucleotide sequence ID" value="XM_007375766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANM3"/>
    <dbReference type="STRING" id="619300.G3ANM3"/>
    <dbReference type="GeneID" id="18873977"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61617"/>
    <dbReference type="eggNOG" id="KOG3771">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072096_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANM3"/>
    <dbReference type="OMA" id="AHDLMAP"/>
    <dbReference type="OrthoDB" id="446293at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990528">
        <property type="term" value="C:Rvs161p-Rvs167p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046982">
        <property type="entry name" value="BIN3/RVS161-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174">
        <property type="entry name" value="BRIDGING INTEGRATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174:SF3">
        <property type="entry name" value="BRIDGING INTEGRATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAR" evidence="6">
        <location>
            <begin position="17"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="83"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="164"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="289" mass="33449" checksum="DABECE9F785B5957" modified="2011-11-16" version="1">MSWIGIKKAINRAGTQVMLKTGHIEQTIDKDYDFEEKRYRTLEASSLKLQKQLRNYSECLKLVTNSQINVAEALSSFYGTTASTAKPKEANGTGEEDNQEQQQQQQQEEAAKFESLSQEYYHTLLKLNSDCVANFEQPYNQTVLNPIARFNSYYIEINDAIKRRTNKQLDYDAMKNKLKKLMEKPQLDPSFDQKVSETASEADEAERAYSEINDQLKRELPKLVSLRVPYFDPSFEAFVKIQTRFFGESYRVLNGLQGKLDARTREDYVSGKLDKRVDEVLDKMRALEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANR5</accession>
    <name>G3ANR5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">T-complex protein 1 subunit eta</fullName>
            <shortName evidence="10">TCP-1-eta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">CCT-eta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_61109</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin. In yeast may play a role in mitotic spindle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterooligomeric complex that forms two stacked rings.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375276.1">
        <property type="nucleotide sequence ID" value="XM_007375214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANR5"/>
    <dbReference type="FunCoup" id="G3ANR5">
        <property type="interactions" value="1453"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANR5"/>
    <dbReference type="GeneID" id="18873716"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61109"/>
    <dbReference type="eggNOG" id="KOG0361">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_7_1_1"/>
    <dbReference type="InParanoid" id="G3ANR5"/>
    <dbReference type="OMA" id="HRKGNTW"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03340">
        <property type="entry name" value="TCP1_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000074">
        <property type="entry name" value="T-complex protein 1 subunit delta, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000022">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000006">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012720">
        <property type="entry name" value="Chap_CCT_eta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02345">
        <property type="entry name" value="chap_CCT_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="544" mass="59450" checksum="039AE0B278C1FBB4" modified="2011-11-16" version="1">MAFSNQTPTIVVLKEGTDASQGRGQILTNINACLAIQETLKPTLGPFGSDILIVNSQGKTTISNDGATILKLLDIVHPAAQMLVDISRSQDAEVGDGTTTVTVLAGELLKEAKTFIEDGVSSHLITRGFRKACELCMHKIEELAVDVRKEDADEFRQLLERCARTAMSSKLISNNSDFFTKMVVDAVLSLDQEELDEKLIGIKKVAGGSIEDSLFIDGVAFKKTFSYAGFEQQPKYFTNPKIVCLNVELELKAEKDNAEVRVEQVKDYQSIVDAEWKIIFNKLEEITSTGANIVLSKLPIGDLATQYFADRNIFCAGRISHEDMDRITKAIGGNVQSTCSDITPADLGTCATFEEVQIGGDRYNLFKGCPEAKTCTLILRGGAEQVIAEVERSLHDAIMIVKRAISNHTVVAGGGAIEMELSKYLRDYSRTIAGKQQLIISAFAKALEVIPRQLCENAGLDAIELLNILRSSHAKGETWYGIDFQKESVGNNMKNFIWEPALVKINAISSATEAATLLLSVDETIRNDEQEQAGPPQGRGRGAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANW1</accession>
    <name>G3ANW1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">xylan 1,4-beta-xylosidase</fullName>
            <ecNumber evidence="14">3.2.1.37</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">1,4-beta-D-xylan xylohydrolase xlnD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Beta-xylosidase A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Beta-xylosidase xlnD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Xylobiase xlnD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">SPAPADRAFT_51129</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Xylan 1,4-beta-xylosidase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Hydrolysis of (1-&gt;4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini.</text>
            <dbReference type="EC" id="3.2.1.37"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycan degradation; xylan degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.37" evidence="14"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375862.1">
        <property type="nucleotide sequence ID" value="XM_007375800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANW1"/>
    <dbReference type="STRING" id="619300.G3ANW1"/>
    <dbReference type="GeneID" id="18871522"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51129"/>
    <dbReference type="eggNOG" id="ENOG502QR4D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004542_5_3_1"/>
    <dbReference type="InParanoid" id="G3ANW1"/>
    <dbReference type="OMA" id="TWNFVED"/>
    <dbReference type="OrthoDB" id="47059at2759"/>
    <dbReference type="UniPathway" id="UPA00114"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046556">
        <property type="term" value="F:alpha-L-arabinofuranosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009044">
        <property type="term" value="F:xylan 1,4-beta-xylosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031222">
        <property type="term" value="P:arabinan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1700:FF:000007">
        <property type="entry name" value="Exo-1,4-beta-xylosidase xlnD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044993">
        <property type="entry name" value="BXL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42721:SF13">
        <property type="entry name" value="EXO-1,4-BETA-XYLOSIDASE XLND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42721">
        <property type="entry name" value="SUGAR HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0858">Xylan degradation</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442628" description="xylan 1,4-beta-xylosidase" evidence="19">
        <location>
            <begin position="19"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type III-like" evidence="20">
        <location>
            <begin position="696"/>
            <end position="766"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00026107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EGW32586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="788" mass="85803" checksum="FA3D275AB0F648F5" modified="2011-11-16" version="1">MLPIKLLATLALSIFAIGETPPSFPDYNTESNPQLPPITFQAVHFSFPDCQNGPLKDNDVCNPYLPNNQRAKAVVDLFTVDELIANMGNTSPGVERLGLPPYQWWSEGLHGIARSNFTASGEYSHATSFPQPILMGGAFNSDLYKQVGNVIGTEARAFNNVGRAGLDYYSPNINPFKDPRWGRGQEVASESPVLVGNYALNYVQGLQGGIDSNPNDDTLQVAATCKHFAGYDMESWKQHSRLGYNAIISDQDLADYYFPTFQSCVRDAKAAGAMCSYNAINGIPVCASEFFLGTVIREGFDFQNGVIHSDCDSLYSIWNPHLYVQDLGAAAADGIKAGVDVNCGDTYQNNLGYALGNKTINEDQIRASVTRQYSNLIRLGYFDSPQTNKYRTYNWSDVSTSQANQLAYQAAVEGITLLKNDGTLPFNKDKVKNVAVIGPWANATTDMLGDYAGTPPYLISPLQGAQDSGFKVQYAYGTQINTTLTTNYTAALNAAKGADAIVYFGGIDNSIENEALDRESLAWPGNQLDLVSKLSGLNKPLVVVQFGAGQVDDTEIKNNNNVNSIVYAGYPGQSGGTAIWDVLNGIYAPAGRLSTTQYPASYADQVPMTDMTLRPRDGYPGRTFMWYNGEPVYEFGYGLHYTTFSVSLANAPPKGAPQSFNIDQFIAAKSSQYVDTSLITTFDVNIKNTGKVTSDYAALLYSNTTSGPGPHPNKILVSFDKLHQIHPGQIQTASLPVTIGSLLQTDTNGNKWLYPGAYTFFVDNDMKAQWEITLTGQAALVQNYPSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANX8</accession>
    <name>G3ANX8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Phosphoacetylglucosamine mutase</fullName>
            <shortName evidence="14">PAGM</shortName>
            <ecNumber evidence="4 14">5.4.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 14">Acetylglucosamine phosphomutase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 14">N-acetylglucosamine-phosphate mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">SPAPADRAFT_61667</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 14">Catalyzes the conversion of GlcNAc-6-P into GlcNAc-1-P during the synthesis of uridine diphosphate/UDP-GlcNAc, which is a biosynthetic precursor of chitin and also supplies the amino sugars for N-linked oligosaccharides of glycoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>N-acetyl-alpha-D-glucosamine 1-phosphate = N-acetyl-D-glucosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23804"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="5.4.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="14 17">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.3" evidence="4 14"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375880.1">
        <property type="nucleotide sequence ID" value="XM_007375818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANX8"/>
    <dbReference type="FunCoup" id="G3ANX8">
        <property type="interactions" value="636"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANX8"/>
    <dbReference type="GeneID" id="18873999"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61667"/>
    <dbReference type="eggNOG" id="KOG2537">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022890_1_0_1"/>
    <dbReference type="InParanoid" id="G3ANX8"/>
    <dbReference type="OMA" id="WEAYATK"/>
    <dbReference type="OrthoDB" id="1928at2759"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00530"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004610">
        <property type="term" value="F:phosphoacetylglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03086">
        <property type="entry name" value="PGM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.50:FF:000003">
        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000013">
        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000023">
        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049023">
        <property type="entry name" value="AMG1_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049022">
        <property type="entry name" value="AMG1_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016657">
        <property type="entry name" value="PAGM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45955">
        <property type="entry name" value="PHOSPHOACETYLGLUCOSAMINE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45955:SF1">
        <property type="entry name" value="PHOSPHOACETYLGLUCOSAMINE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21405">
        <property type="entry name" value="AMG1_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21404">
        <property type="entry name" value="AMG1_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016408">
        <property type="entry name" value="PAGM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 14 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 14 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="19">
        <location>
            <begin position="57"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="19">
        <location>
            <begin position="121"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoacetylglucosamine mutase AMG1" evidence="21">
        <location>
            <begin position="180"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoacetylglucosamine mutase AMG1" evidence="20">
        <location>
            <begin position="298"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="18">
        <location>
            <begin position="456"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="15">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="binding site" description="via phosphate group" evidence="17">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="374"/>
            <end position="376"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="501"/>
            <end position="505"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="510"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR016408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF21404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF21405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="EGW32604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="533" mass="58801" checksum="604810AB220AAAE8" modified="2011-11-16" version="1">MSIEQTLGQYLPSHPKPDGLKFTYGTAGFRMKAVNLDYVNYTTGIIAALRSKFLQGQTVGVMITASHNPPEDNGVKVVDPLGSMLQTSWEAYATTLANSTHEELLGNISKLVEDLGIDLSAPASVVIARDSRESSERLSNATIDGFKSVPNTKYQDFGLFTTPQLHYVTRTINDPAFGATSEEGYYAKMAESFKKIFALSGNSEKIDITIDAANGVGAPKIKELLATYLSEQITFNLVNGNYNSPQLLNFDCGADYVKTNQKLPKNVTPTPNKLYASFDGDADRLICYYIDPEFRLLDGDKLSTLIALFLQQLFKHIDSAKLKLDIGVVQTAYANGSSTKYVEDVLKIPVRCTPTGVKHLHHEAENFDIGVYFEANGHGTVIFKPEAEQKIFEYKADPVNAEEVKSIQVLQEFSKLINQTVGDAISDLLAVLIIVHYLQLTPRDWNKCYNDLPNKLVKVIVPDRSVFKTTNAERTLVEPTGLQDKIDAEVAKYNHGRSFVRASGTEDAVRVYAEADTKDNVEALAKAVADLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP80</accession>
    <name>G3AP80_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Mitogen-activated protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_139389</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375927.1">
        <property type="nucleotide sequence ID" value="XM_007375865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP80"/>
    <dbReference type="FunCoup" id="G3AP80">
        <property type="interactions" value="285"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP80"/>
    <dbReference type="GeneID" id="18870205"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139389"/>
    <dbReference type="eggNOG" id="KOG0660">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="InParanoid" id="G3AP80"/>
    <dbReference type="OMA" id="GLYCFQE"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070056">
        <property type="term" value="C:prospore membrane leading edge"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904853">
        <property type="term" value="P:protein localization to ascospore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000040">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="30"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="375" mass="43369" checksum="08837AAE7FA581C7" modified="2011-11-16" version="1">MSSVKNRNGYMMSCSTYTDNENHFLVDSRYEIIKVLGKGSYGVVCLAIDAKSLNSGHEHKIAIKKVTKIFNKDILLIRAIRELKFMKFFRGHKNISTLIDLDVVYVKPYDGLYCFQELADLDLARVLYSNVQFSEFHIQNFMYQIMCGIKYIHSADVIHRDLKPGNILVTTQGILKICDFGLARGINPKYFKNRSATITNYVATRWYRAPELILSTKNYTKAVDIWAAGCILGELFGRRPLFPGKNSNEQIHELFKILGNPPLDTVKKYNWKVEGLLWVKYKPVKWKSLYPFAPHEAINLLEKLLQWDYKKRLEVEQILGHAFFRNTRNASDEPVSKELFDFSFEGRANNISQLKDLLELEVKHFKDTCKVGLDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP87</accession>
    <name>G3AP87_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein-tyrosine phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_56058</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375934.1">
        <property type="nucleotide sequence ID" value="XM_007375872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP87"/>
    <dbReference type="FunCoup" id="G3AP87">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP87"/>
    <dbReference type="GeneID" id="18871995"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56058"/>
    <dbReference type="eggNOG" id="KOG1572">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047845_4_0_1"/>
    <dbReference type="InParanoid" id="G3AP87"/>
    <dbReference type="OMA" id="HYPCYIH"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17663">
        <property type="entry name" value="PFA-DSP_Oca6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000084">
        <property type="entry name" value="Tyrosine phospatase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF14">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE OCA6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="23023" checksum="0FD7C3B248B3C452" modified="2011-11-16" version="1">MSLIPEPLIPPLRFNSIEANLYRGAYPREVNLPFLETLQLKTIISLTPNPITLETDAKLYQFAHEHKIRLVHLECAPAGKGKKRGVPLEYHTILTAINFMIHKKHQPVYIHCLNGGQVTSLVVACLRKLQFWSLISIFNEFINFTTNITVNDRSFVEGFKGEINIDPDDKVDWLWVGVSKHVIGTHPKLKVIEAKSFQDVTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP95</accession>
    <name>G3AP95_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab GDP dissociation inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61735</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Rab GDI family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375942.1">
        <property type="nucleotide sequence ID" value="XM_007375880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP95"/>
    <dbReference type="FunCoup" id="G3AP95">
        <property type="interactions" value="839"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP95"/>
    <dbReference type="GeneID" id="18874026"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61735"/>
    <dbReference type="eggNOG" id="KOG1439">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021695_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP95"/>
    <dbReference type="OMA" id="FETKAKM"/>
    <dbReference type="OrthoDB" id="9446342at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005093">
        <property type="term" value="F:Rab GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.405.10:FF:000001">
        <property type="entry name" value="Rab GDP dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.519.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018203">
        <property type="entry name" value="GDP_dissociation_inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000806">
        <property type="entry name" value="RabGDI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787:SF8">
        <property type="entry name" value="RAB GDP DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787">
        <property type="entry name" value="RAB GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00996">
        <property type="entry name" value="GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00892">
        <property type="entry name" value="RABGDI"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00891">
        <property type="entry name" value="RABGDIREP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="449" mass="50851" checksum="DA5ADEBE75EA498F" modified="2011-11-16" version="1">MDENYDVIVLGTGLTECILSGILSVEGKKVLHIDRQDFYGGESTSLNISQLYSKFKPNGQKPELKGRDRDWCIDLIPKFLMANGELTNILVHTDVTRYIEFKQIAGSYVYRNGRIAKVPANEMEAVRSSLMGIFEKRRMKQFLQFIQNYDDEDPKTHHGFDLDKNTMNEIYTYYGLENGTKDFIGHAMALWHTDDYLNEVARPTYERIILYVQSVAKYGKSPYIYPLYGLGELPQGFARLSAIYGGTYMLDTPIDEVLYEGEGEDKKFAGVVTKEGTAKAPIVIADPTYFPELVKKVGEKVIRAICLLDHPVPGVDLDSLQLIIPQNQVGRKHDIYVAVLSDVHCVVPKGYYLAIISTIIETDQPHIELEPAFKLLGPRIDTLMGIAELYEPINDGTKNGIYISKSYDSSSHFEQTTDDVKDIYFRITGKPLELKKRPTAEEEEQLQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3APS2</accession>
    <name>G3APS2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Alpha-1,3-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_50813</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375519.1">
        <property type="nucleotide sequence ID" value="XM_007375457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APS2"/>
    <dbReference type="FunCoup" id="G3APS2">
        <property type="interactions" value="60"/>
    </dbReference>
    <dbReference type="GeneID" id="18871487"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50813"/>
    <dbReference type="eggNOG" id="ENOG502RZ48">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015387_0_1_1"/>
    <dbReference type="InParanoid" id="G3APS2"/>
    <dbReference type="OMA" id="CEMYTIC"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000033">
        <property type="term" value="F:alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392:SF1">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="578" mass="66791" checksum="E47A3377E501B248" modified="2011-11-16" version="1">MGINERCDQFFKILHQLDPNWCVDPSNNFNTRDFKSSEFQNYIKDKKEPINNLEKEFSTKKFKENGPKIRGLLSIFRIFNKCYIKSTNNRFNWNPNSFLHGSFGLGKPANSFFEGTHTFEKRIYPWLSGESPTYENWNGEVYFDLPGSNSRSAINDPYWLNIFREKSNGRGIVLTIGNKHVDNTVRLIRVLRALKNKYPIQILVDEDFSQESKHNIISAARDDFTELPKSYENVSDILGKNYLKPGESLPWQEIWFINVKNAIVPSYWNLFQGFTKKLLASIFNSFEEYMLLDSDSVILENPEFFFNIKEYQQTGAYFYKDRAWKARAKKSSNFLKTLGPNLIDSVVFDIPMMTNHTLDREIFKGFRHYMESGLVLLNKKQHFNSVLMSLQLSFFEPISSQFYGEKEYFWLGLAFNGDESYEFDPNFSASIGNFSDTNNPIKEVCSAHPAHLSAHDGHLLWLNSGFRFCHKAGIGKVKYDVEAGHNGRFPSNPHELKSIFESLFRPSHGIIPPWSLDMKGKNGQFLTGWNDEFKYCAGTMFCAVSSVGSIDKPILGQVITFDDTAKNLMSYYGEIWMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3APX4</accession>
    <name>G3APX4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription initiation factor IIE subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_153024</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TFIIE beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375571.1">
        <property type="nucleotide sequence ID" value="XM_007375509.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APX4"/>
    <dbReference type="FunCoup" id="G3APX4">
        <property type="interactions" value="491"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APX4"/>
    <dbReference type="GeneID" id="18870940"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_153024"/>
    <dbReference type="eggNOG" id="KOG3095">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056580_2_0_1"/>
    <dbReference type="InParanoid" id="G3APX4"/>
    <dbReference type="OMA" id="RTKKDNH"/>
    <dbReference type="OrthoDB" id="5323195at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005673">
        <property type="term" value="C:transcription factor TFIIE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001097">
        <property type="term" value="F:TFIIH-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07977">
        <property type="entry name" value="TFIIE_beta_winged_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054600">
        <property type="entry name" value="TFA2_E-tether"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040501">
        <property type="entry name" value="TFA2_Winged_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016656">
        <property type="entry name" value="TFIIE-bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003166">
        <property type="entry name" value="TFIIE_bsu_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12716:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12716">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22254">
        <property type="entry name" value="TFA2_E-tether"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18121">
        <property type="entry name" value="TFA2_Winged_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02186">
        <property type="entry name" value="TFIIE_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016398">
        <property type="entry name" value="TFIIE-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51351">
        <property type="entry name" value="TFIIE_BETA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TFIIE beta" evidence="9">
        <location>
            <begin position="72"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="16"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="246"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="253"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR016398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="288" mass="31950" checksum="0414562F8B3BB442" modified="2011-11-16" version="1">MSDLSAQLSAFKNKIKSGPSVTAARKPVLNKVPSPISSPVKKNGDTSNGVVKRSGASSSDSMTEVLKRQKLSSNSIGEMSGSHLSTQLHLAVEFIKEHDAPVPVSKLQNYLSFDITHTLLPLLREIDRVKYDANNGTLEYVSLHNIRSADDLLEFLRRQTTFKGTSVKELKDGWAGCLTAINELEAENKILVLRNKKENAPRLVWANAGGELGTIENEFKDMWVKVKLPEPDALYQALLDQGLKPTGADPNLIKKKPQQQEKKQKKARRGRITNVHMKGILKDYSQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ24</accession>
    <name>G3AQ24_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_61424</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375621.1">
        <property type="nucleotide sequence ID" value="XM_007375559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ24"/>
    <dbReference type="GeneID" id="18873887"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61424"/>
    <dbReference type="HOGENOM" id="CLU_2672679_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQ24"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="75" mass="8758" checksum="13E8A9B44343C81D" modified="2011-11-16" version="1">MHTMNKLQGGTTNNNNRTQDESVWKIARNVAKLFLVPYFILFFWGKSSRDRTSGETPSSYTFGFKPFSGYILFYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ28</accession>
    <name>G3AQ28_SPAPN</name>
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        <recommendedName>
            <fullName evidence="4">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_50913</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EGW32349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ28"/>
    <dbReference type="GeneID" id="18871499"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50913"/>
    <dbReference type="eggNOG" id="KOG0691">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025145_3_1_1"/>
    <dbReference type="InParanoid" id="G3AQ28"/>
    <dbReference type="OMA" id="RPALMDK"/>
    <dbReference type="OrthoDB" id="552049at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000028">
        <property type="entry name" value="DnaJ domain protein"/>
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    </dbReference>
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        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DnaJ_X"/>
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        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Peroxisomal_DnaJ"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45006:SF1">
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    </dbReference>
    <dbReference type="Pfam" id="PF00226">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14308">
        <property type="entry name" value="DnaJ-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
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    </dbReference>
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        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="4">
        <location>
            <begin position="6"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="118"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="316"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="457"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="169"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="141"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="485" mass="54970" checksum="5B115BDE3C36847C" modified="2011-11-16" version="1">MVVDTTYYDLLGIETTATSLEIKKAYRKAAIRLHPDKNPNDPTAAARFQEVGQAYQVLSDDTLRAKYDKFGKQESIPTEGFEDPAEFFSMIFGGEAFKEWIGELSLLSELSKSAELAGYTDGEDDKKEKSDGDKTEPGVNETSTEGATTSTSGTTNPSKDQLLLSHTTHDESKLTAKEIEESKRKAELEKFEEECRLKKLETRRQLAQKLVEKLSLFTETDMKQDVIDSFQTKIKYEAESLKMESFGLEILHTLGHIYKTKSRIFFKNQTFFGWGGFWYSVKEKGGVVKDTFKTVSSALDAQRTMEEYTQMQQDNEYHAKKEAEEQKEQDPEEAKSAAEQEVNKLEEELEHVKQEKAEVEKSKEPIPNKHTPEELAEMEKYLMGKVLAAAWSGSKFEIQGTVRAVCDMVLEDKEVPLETRVARAKGLRLIADVFIAVNRTEDEAEEARVFEELVAEASKKRDRKKHHHNDEDVAKNDVPAEAGAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ61</accession>
    <name>G3AQ61_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_61969</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376186.1">
        <property type="nucleotide sequence ID" value="XM_007376124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ61"/>
    <dbReference type="STRING" id="619300.G3AQ61"/>
    <dbReference type="GeneID" id="18874131"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61969"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083654_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQ61"/>
    <dbReference type="OMA" id="PRIYNCK"/>
    <dbReference type="OrthoDB" id="10018191at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF11">
        <property type="entry name" value="ZINC FINGER PROTEIN YPR022C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="46"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="75"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="212" mass="23382" checksum="5F338D02C3848432" modified="2011-11-16" version="1">MSSPNSPISPVGSPLTESSSTDSSILTTANTANYGTKTAGGNPRIYNCKMCSRAFTREEHLTRHTLSTHNKLKPFTCGLCSRPFSRRDLLLRHAKNLHQGSELAVSRIRKSYKREHDSQRSQSISDEELPTNRFNITFANKNYKTDSIMPVPPVQVANPSMMTAAATTTTTTTISPATTSTPPVVPTVKKSFDDFDTPEKKRLKMSVDMLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ95</accession>
    <name>G3AQ95_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pal1 cell morphology protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62006</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376220.1">
        <property type="nucleotide sequence ID" value="XM_007376158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ95"/>
    <dbReference type="STRING" id="619300.G3AQ95"/>
    <dbReference type="GeneID" id="18874147"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62006"/>
    <dbReference type="eggNOG" id="ENOG502QPHY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041195_1_0_1"/>
    <dbReference type="InParanoid" id="G3AQ95"/>
    <dbReference type="OrthoDB" id="5352132at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013226">
        <property type="entry name" value="Pal1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28307">
        <property type="entry name" value="PROTEIN PAL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28307:SF2">
        <property type="entry name" value="PROTEIN PAL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08316">
        <property type="entry name" value="Pal1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="432"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="259"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="442"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="49658" checksum="669630A125105CF0" modified="2011-11-16" version="1" fragment="single">MYQQPNHSYASADSLRRFSSNNPFRNQIDQRSVQPQNSGSSLSNSNKNRSPSKSSLGQSSITFENWIQKNKDLLALSSDEEEDEAPINRDDDILNVYQSNGGTPLTSHNNSTSGTPRPPQFPRATRADSDTSIHYSNRTMQSNNPFASALNEPDSYVHRREPPAPPRRGSSGNTPPARPPKPPADSLPPPSYEEAAGREAARREYRREKASSSSNDNNLAPPSRPHRSHSDSNGHGRERSRGHRSHHHSRKDAKPESQPPSSRTLKASSSLSRKKSPSKKTPEPVKAKNLDTIDKLDVTAFFGGGFHHDGPFDACAPHRNKNVKAAPVMAFPVDGPNNYIGGSGGNIDKNEQMDLAFGNYNADRDDATFRRPGATMRTGRLSDDGPVTAGLKTNRVHDDPSSTIYTAKQNPSVINFDSNLKAQPIHGSTTAGLGSTTFVDGAPAPKKAAQEAEQQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ96</accession>
    <name>G3AQ96_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_62007</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376221.1">
        <property type="nucleotide sequence ID" value="XM_007376159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ96"/>
    <dbReference type="STRING" id="619300.G3AQ96"/>
    <dbReference type="GeneID" id="18874148"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62007"/>
    <dbReference type="eggNOG" id="KOG1729">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_923062_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQ96"/>
    <dbReference type="OrthoDB" id="5352132at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="6">
        <location>
            <begin position="143"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="40"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="93"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="302" mass="34497" checksum="0C568079438D8BEA" modified="2011-11-16" version="1" fragment="single">MSSELNEADGLLLEQYTANNYIYERKMNRDKLRAIMNDKTPIAAPRQPPRLRNQSSFPKLKDNSPRSLHQVYELKKPLLTPAVLRPAYTNEESSTSSSQTPSQLTVSRSQIETPPLKTVYTVSAPESSVELDIEPIHTHWKSNTTTKNCMSCFKPFRNSLLSLIYDVSNKRHHCRFCGFIFCIDCLVQDNETSYNQSVTLLDARAQFVIPVFKNLDQIPSQGYKSFKVCKKCNIDYNALVEDINRSLEDGEWKYLSNSTRDDLKKLPFICVENPYVGNTTRFRVNTDSANKQEGFRGKASGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQB7</accession>
    <name>G3AQB7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_141348</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAK10 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376242.1">
        <property type="nucleotide sequence ID" value="XM_007376180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQB7"/>
    <dbReference type="FunCoup" id="G3AQB7">
        <property type="interactions" value="616"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQB7"/>
    <dbReference type="GeneID" id="18870367"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141348"/>
    <dbReference type="eggNOG" id="KOG2343">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022669_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQB7"/>
    <dbReference type="OMA" id="ITWEFAD"/>
    <dbReference type="OrthoDB" id="269405at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057983">
        <property type="entry name" value="NAA35-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007244">
        <property type="entry name" value="Naa35_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057982">
        <property type="entry name" value="TPR_NAA35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373">
        <property type="entry name" value="GLUCOSE REPRESSIBLE PROTEIN MAK10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373:SF0">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 35, NATC AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04112">
        <property type="entry name" value="Mak10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25789">
        <property type="entry name" value="TPR_NAA35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAA35-like N-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="NAA35-like TPR repeats" evidence="5">
        <location>
            <begin position="172"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW31464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="622" mass="71821" checksum="69AF144E78B07FC2" modified="2011-11-16" version="1">MGLCKFIDFVLHIAKNLLYEEEDITTRNMNLDFLSGISPQFLIQEINEVIEWIIVNEVDQANVIIAQLKVVSNLMKIEATILATPIQFLNKDDNISLNLNFYTEGISQVGQLQNVEYDESVIPEGAFSKFIQLDMENKNIPLELNDVPLDTALTYIKAIFETVERFIREANEINSINQLQDYLEYNINYPIDTFSVFSRGLFQLFFIRDDKSIFGSLEYNLTNLSTELIENLVGKNTIVLNNLESSLSQLKDSTRNDLMSRYVGVLQDMEGGIYHNLTVFGCNPCRQQQLISKGLVIWDTLQVGWESFEVDLHQSYGIGDEFATGEVSISVTSYIYYTKIKMMMQLLLNGFELELYKPFEIYLILWYGDYLAQNLVDHLQNRVTRIIIGKIEHLETVIPKRIKKLKAGVKKEQLKELNKYNHEVILPQLTATLNYNQDYLTKSYQTLLTLLRANMKYLATLSKIGLIDFTKGPSHSLTSLEHLYYLRLKPWSSIGVPNFPTYQNYQQIITATLPERDNRSTLFRCLELLASSKSGLATCRKEYEIILGYIKRDTRNFVSNSKVNEWYHEFISSIDVLEENIGLLSKLISQNKDDLSKINKEYKIKVDKGVHRYIPNLSVVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQE4</accession>
    <name>G3AQE4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ML-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_62061</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transient receptor potential (TRP) ion channel family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376269.1">
        <property type="nucleotide sequence ID" value="XM_007376207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQE4"/>
    <dbReference type="FunCoup" id="G3AQE4">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQE4"/>
    <dbReference type="GeneID" id="18874174"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62061"/>
    <dbReference type="eggNOG" id="ENOG502QSVZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010226_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQE4"/>
    <dbReference type="OMA" id="SIMGWVF"/>
    <dbReference type="OrthoDB" id="5212126at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010308">
        <property type="entry name" value="TRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040241">
        <property type="entry name" value="TRP_Flc/Pkd2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032800">
        <property type="entry name" value="TRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145:SF4">
        <property type="entry name" value="FLAVIN CARRIER PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_7G01610)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06011">
        <property type="entry name" value="TRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14558">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01320">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="242"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="407"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="468"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="ML-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="570"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="606"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="656" mass="73378" checksum="2128F220C053A25B" modified="2011-11-16" version="1">MCTLGWKQFCPVYPGPLQVESIEYISEDYANQIPGIAYQVPDIDAVVKVLVKDRDTQETLACIQSSFSNGKTISQTGVKWATAVVAGLGLLLAALLSTFGNSNAASHISANSVSLFLYFQSVVVVSMQSVERVPPIASAWSENLAWSMGLIRVQFMQDIFRWYVQATGGTPTTYFKGIIQQILVQKRNLQAMVMDVMTRSNLTKRTLDFALKSNKNLIVLRGIKRIGYNSGIEPTSIVVTGFTFFVLIGYLLVVVLVLAKSVIVLLGRFNKINPKSFSMFRTNFNNMIKGTVLRYIAIGFTQLTLFALWEFVQRDSPAVIVIAVLFIILLAAVLSWSYYNVIRLGRESQQLYQNPAALLYGDTHILQKYGFSYTMFTAKKYYFGVVLAGYTLLKSIFIAFTQSSGRVSVLPIFIFDLAYTIYLIYSAPYLNKPTNIVNYMISIVVTINSFLFLFFSDLFGLQASVASIMGWIFFILNAAFSLILLVLIVVFIVFCLVSKNPDARFAPVKDDRFSFQRKPSMHRTVRSPIMTEKNNVEGELAALGAIAQDHSVDWESEMYKLNNVANDSVDEDILTGSSQPDLEEKERAPPSRFGSLKQKFMNRVGMNKEEIKPTDESSPIREDVDSDILSALQGHKKTISQASSDYSDPTDAKRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQF5</accession>
    <name>G3AQF5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial pyruvate carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_62077</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the uptake of pyruvate into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376280.1">
        <property type="nucleotide sequence ID" value="XM_007376218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQF5"/>
    <dbReference type="FunCoup" id="G3AQF5">
        <property type="interactions" value="483"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQF5"/>
    <dbReference type="GeneID" id="18874179"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62077"/>
    <dbReference type="eggNOG" id="KOG1590">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099502_3_2_1"/>
    <dbReference type="InParanoid" id="G3AQF5"/>
    <dbReference type="OMA" id="CTTHFWG"/>
    <dbReference type="OrthoDB" id="1697690at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:7770001">
        <property type="term" value="C:mitochondrial pyruvate carrier complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050833">
        <property type="term" value="F:pyruvate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006850">
        <property type="term" value="P:pyruvate import into mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005336">
        <property type="entry name" value="MPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14154">
        <property type="entry name" value="UPF0041 BRAIN PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03650">
        <property type="entry name" value="MPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="122" mass="13775" checksum="3615835538645E6D" modified="2011-11-16" version="1">MSGFKKFTDFLFSKKSLKYMCTTHFWGPVSNFGIPIAAILDLKKDPNLISGPMTGSLILYSLVFMRYSMAVTPQNYLLFGCHFVNELAQLGQGFRWVKCHYVDEPKPAVVAAIEDKVNEVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQG2</accession>
    <name>G3AQG2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA damage checkpoint protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_154762</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376287.1">
        <property type="nucleotide sequence ID" value="XM_007376225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQG2"/>
    <dbReference type="FunCoup" id="G3AQG2">
        <property type="interactions" value="145"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQG2"/>
    <dbReference type="GeneID" id="18871000"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_154762"/>
    <dbReference type="eggNOG" id="ENOG502QT39">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036738_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQG2"/>
    <dbReference type="OMA" id="EHYCLFT"/>
    <dbReference type="OrthoDB" id="3992718at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071479">
        <property type="term" value="P:cellular response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007268">
        <property type="entry name" value="Rad9/Ddc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15237:SF0">
        <property type="entry name" value="CELL CYCLE CHECKPOINT CONTROL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15237">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04139">
        <property type="entry name" value="Rad9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="369"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="491" mass="56135" checksum="A2A00CD1232A4C3D" modified="2011-11-16" version="1">MTFIAIIDSKSHHLMWSKSILSLSAISEFIKFVITKNSFSISAVNNSNTSHAEIIFKSSFFNEYHVDFSGILPEGYEEGIEDIDSDDQSSYSFIVNSRHLATVFRTIEPSTLLYVCFKINWRIDSSISQRYKLSIEIKTKKLIIKKYQTSYQPVLRGNMHIVQSYKKQLYDQPHDSFDGPNRINHLVIDQLIPKQFLEMVPPSAEDFKIDIKNGKISFSGYTKQIIKDRDYLKQPMAVTVTISLEDLIDSNLITTGTDPIKKCINFRLRDFKNFLNLINFFTSSNSQKDQFDENYMTLPSADNFDMFFRNAGDPILFELQNSNHVSIQYIQITSDDKATDIEAKQVNVNITKRQSMSLQSHIIQRIENRDIANSRSTSASPMKNNSSIRTPVSKLPSTSSLRHSIRLPSGWRTPERDLNSDFGGNNDEEYITYGKEPSPLKHSKTPLKQNSADADTDYSDSEDEPPRKRSNIVEEDGFGPTQLGNKPKSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQI2</accession>
    <name>G3AQI2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62117</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376307.1">
        <property type="nucleotide sequence ID" value="XM_007376245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQI2"/>
    <dbReference type="STRING" id="619300.G3AQI2"/>
    <dbReference type="GeneID" id="18874190"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62117"/>
    <dbReference type="eggNOG" id="ENOG502SACF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_931168_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQI2"/>
    <dbReference type="OrthoDB" id="4089169at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="299" mass="33829" checksum="B68FE7A4570D788D" modified="2011-11-16" version="1">MGTFKPPGGISQLSLSPYDLQLVHANARGDSLYMWDLYRLPVEISLIGKFSRGKTSAIIEEIFWFINNYENKRDEGEVFGNNSGFGCISKSTGSLHWFNINYLAGSYNNNRPNSFKKTSFAPGKFMDSWILSSLKAKQFVSIPTNQCSNLNQLAIIDENLVLKLISPLNGHHFYQFQLSKVPVDSQFVPKTSSIEKPHIPSSPYVNPLSQAEIETCAPFLNLINNPRIEFATFEDDNLEEIFSEFGNMVPEQVITFRSKPKYRTLKPKDIKDNDLPSVDTLFIDQDEASDDSKDDSWTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQJ0</accession>
    <name>G3AQJ0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_62128</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Part of the multisubunit transport protein particle (TRAPP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus</location>
            <location evidence="6">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAPP small subunits family. BET5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376315.1">
        <property type="nucleotide sequence ID" value="XM_007376253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQJ0"/>
    <dbReference type="FunCoup" id="G3AQJ0">
        <property type="interactions" value="514"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQJ0"/>
    <dbReference type="GeneID" id="18874193"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62128"/>
    <dbReference type="eggNOG" id="KOG3368">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053380_4_0_1"/>
    <dbReference type="InParanoid" id="G3AQJ0"/>
    <dbReference type="OMA" id="GKLMYGM"/>
    <dbReference type="OrthoDB" id="3364529at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007233">
        <property type="entry name" value="TRAPPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249:SF16">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04099">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01399">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="4 6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="160" mass="18302" checksum="A504D5B72835DCE1" modified="2011-11-16" version="1">MTIYSFFIFDRHCNCIYNREFTQTSAAGVNNGLINKNNTSHSSKLLFGIIYSLKSLSAKLISENELTSFTLGQSFRFHFWESVTSYKFVVVTGYDVDDMQQVLVRLHQYFVDCVASNGLTPVEFKWSANGEKLDNVGYSVICNNSFVEETDQYLQSLSIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR01</accession>
    <name>G3AR01_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">SAGA-associated factor 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SGF11</name>
        <name evidence="12" type="ORF">SPAPADRAFT_62278</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Functions as component of the transcription regulatory histone acetylation (HAT) complex SAGA. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction and promoter selectivity, interaction with transcription activators, and chromatin modification through histone acetylation and deubiquitination. SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). Involved in transcriptional regulation of a subset of SAGA-regulated genes. Within the SAGA complex, participates in a subcomplex, that specifically deubiquitinates histones H2B.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10 11">Component of the 1.8 MDa SAGA transcription coactivator-HAT complex. SAGA is built of 5 distinct domains with specialized functions. Within the SAGA complex, SUS1, SGF11, SGF73 and UBP8 form an additional subcomplex of SAGA called the DUB module (deubiquitination module). Interacts directly with SGF73, SUS1 and UBP8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The C-terminal SGF11-type zinc-finger domain together with the C-terminal catalytic domain of UBP8 forms the 'catalytic lobe' of the SAGA deubiquitination module.</text>
    </comment>
    <comment type="domain">
        <text evidence="10">The long N-terminal helix forms part of the 'assembly lobe' of the SAGA deubiquitination module.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the SGF11 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376440.1">
        <property type="nucleotide sequence ID" value="XM_007376378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR01"/>
    <dbReference type="FunCoup" id="G3AR01">
        <property type="interactions" value="277"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR01"/>
    <dbReference type="GeneID" id="18874238"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62278"/>
    <dbReference type="eggNOG" id="ENOG502SG7T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130538_0_0_1"/>
    <dbReference type="InParanoid" id="G3AR01"/>
    <dbReference type="OMA" id="RYFSCEN"/>
    <dbReference type="OrthoDB" id="21557at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071819">
        <property type="term" value="C:DUBm complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03047">
        <property type="entry name" value="Sgf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013246">
        <property type="entry name" value="SAGA_su_Sgf11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08209">
        <property type="entry name" value="Sgf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0010">Activator</keyword>
    <keyword evidence="5 10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="SGF11-type" evidence="10">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU261113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="106" mass="11968" checksum="7188AA4D13E4A83B" modified="2011-11-16" version="1">MTEGVTVQELAESIFDDLVLAIINQQTLTSYANHTSIKSQTARNEEQVQQITNLEEGSKLDVYGQDKIKLKSAETSRYFSCENCGRKIAGGRFASHVNKCLERRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR51</accession>
    <name>G3AR51_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sof1-like protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_61803</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat DCAF13/WDSOF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376004.1">
        <property type="nucleotide sequence ID" value="XM_007375942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR51"/>
    <dbReference type="FunCoup" id="G3AR51">
        <property type="interactions" value="1308"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR51"/>
    <dbReference type="GeneID" id="18874057"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61803"/>
    <dbReference type="eggNOG" id="KOG0268">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033999_0_1_1"/>
    <dbReference type="InParanoid" id="G3AR51"/>
    <dbReference type="OrthoDB" id="10249065at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001105">
        <property type="entry name" value="WD40-repeat-containing domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007287">
        <property type="entry name" value="Sof1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051733">
        <property type="entry name" value="WD_repeat_DCAF13/WDSOF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22851:SF0">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22851">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA U3 SNORNA ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04158">
        <property type="entry name" value="Sof1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="11"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="34"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="130"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="216"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="259"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Sof1-like protein" evidence="8">
        <location>
            <begin position="292"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="333" mass="37592" checksum="16CC561E539E662C" modified="2011-11-16" version="1">MARDFSSTSQFASGSGDGVIKYWNLTSRDEIASFRAHVGMVSGLVVCKEGKMLSCGDDKTIKLWSVDSEEFDKKVGDDEIYSGSQGLIKTFVGDAAFKGIDRHRNDPIFVTGGATIQLWDMNRSKYTSDLSWGADNVTTVKFNQTETNIIASAGSDNSVVLYDVRTNTAIQKVVLSLRTNSISWNPMEAFNFATGSDDHNAYLWDMRNLGRSLNVYKDHVAGIMDVDFSPTGEELVTGSYDKTIRIFKTRSGHSRDIYHTKRMQRIFCVKYSSDARYILSGSDDTNVRVWRANASDRANIKSARQRSKMEYDNALKERYKHMPEIKRISRHLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR74</accession>
    <name>G3AR74_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_56134</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376027.1">
        <property type="nucleotide sequence ID" value="XM_007375965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR74"/>
    <dbReference type="STRING" id="619300.G3AR74"/>
    <dbReference type="GeneID" id="18872002"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56134"/>
    <dbReference type="eggNOG" id="KOG0114">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_25_3_1"/>
    <dbReference type="InParanoid" id="G3AR74"/>
    <dbReference type="OMA" id="VLYYRSN"/>
    <dbReference type="OrthoDB" id="275748at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051847">
        <property type="entry name" value="RNA_proc/Spliceosome_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45880">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN, X-LINKED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45880:SF1">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN, X-LINKED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="6"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="109" mass="12551" checksum="A34C308B61FEB69E" modified="2011-11-16" version="1">MSREFPIVLVKNLPYKVSTSSMYEFFGQFGNINQIRISNEESTQGSCFIIYSNLANAIRAANELNGVNFQGRYLVTSLYPVDKSLINKEEMILRKEQLSQLKQQYSIQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARC0</accession>
    <name>G3ARC0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="4">PPIase</shortName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_62331</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376504.1">
        <property type="nucleotide sequence ID" value="XM_007376442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARC0"/>
    <dbReference type="FunCoup" id="G3ARC0">
        <property type="interactions" value="238"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARC0"/>
    <dbReference type="GeneID" id="18874273"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62331"/>
    <dbReference type="eggNOG" id="KOG0880">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_4_1_1"/>
    <dbReference type="InParanoid" id="G3ARC0"/>
    <dbReference type="OMA" id="ENHEITH"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000001">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5006524887" description="Peptidyl-prolyl cis-trans isomerase" evidence="4">
        <location>
            <begin position="25"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="6">
        <location>
            <begin position="37"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="198"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="233" mass="25515" checksum="D7BBCA52599F3096" modified="2011-11-16" version="1">MKLVTAFALIASLIYFFLGTGASAAAPENPPVTNKVFFDIEEDGKPIGRITIGLFGTIVPKTVENFRQLAISTDPKFGYTGSIFHRVIPKFMIQGGDYETGQGYGGKSIYGGKFEDENFELKHDRKYRLSMANAGRNTNGSQFFITTALTKWLDNAHVVFGEVLDGFNVVDYIENVKTSRGDRPVKEIKIAKSGELPVENTGATSAAESSAEENTVTETPKQVVDDEVPKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARC4</accession>
    <name>G3ARC4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SP-RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_62332</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NSE2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376509.1">
        <property type="nucleotide sequence ID" value="XM_007376447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARC4"/>
    <dbReference type="FunCoup" id="G3ARC4">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARC4"/>
    <dbReference type="GeneID" id="18874274"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62332"/>
    <dbReference type="eggNOG" id="KOG2979">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088986_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARC4"/>
    <dbReference type="OMA" id="IELICPI"/>
    <dbReference type="OrthoDB" id="756301at2759"/>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16651">
        <property type="entry name" value="SPL-RING_NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026846">
        <property type="entry name" value="Nse2(Mms21)"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21330">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21330:SF1">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11789">
        <property type="entry name" value="zf-Nse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SP-RING-type" evidence="11">
        <location>
            <begin position="181"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="279" mass="32732" checksum="4083ED003BB3FC9E" modified="2011-11-16" version="1">MSDSEEEVPTMELKLPDYLPLKSELMRDFEKSFHNRTIPDIIKVQTRQIQEAAEKYIQYVLEHPQLDFDESILSSFEKSLENLIRSEFEFSKLEAIRKNPSVSAFASREESDLTLDSLESYQNITKPNFSDVIEEQYLEMKSPKYSKGYKDLIHKHKFYNYLNDILFVIRNPEDQIPDENEDEELNVSGGKISLKDPLSLNYYSDPVRSKRCKHTFERSNIEEQLKRGEASRERITHCPIAACSQVINRADLEADVLMIIRIRSFQRLHNKTRDVDTVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARE0</accession>
    <name>G3ARE0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Histone-lysine N-methyltransferase, H3 lysine-79 specific</fullName>
            <ecNumber evidence="2 11">2.1.1.360</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 11">Histone H3-K79 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_140015</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Histone methyltransferase that specifically trimethylates histone H3 to form H3K79me3. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60328"/>
            <dbReference type="Rhea" id="RHEA-COMP:15549"/>
            <dbReference type="Rhea" id="RHEA-COMP:15552"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.360"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="11">Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="11">In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.360" evidence="2 11"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376525.1">
        <property type="nucleotide sequence ID" value="XM_007376463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARE0"/>
    <dbReference type="STRING" id="619300.G3ARE0"/>
    <dbReference type="GeneID" id="18870264"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140015"/>
    <dbReference type="eggNOG" id="KOG3924">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004528_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARE0"/>
    <dbReference type="OMA" id="NFIPPRY"/>
    <dbReference type="OrthoDB" id="443402at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140956">
        <property type="term" value="F:histone H3K79 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.170">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025789">
        <property type="entry name" value="DOT1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030445">
        <property type="entry name" value="H3-K79_meTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451">
        <property type="entry name" value="HISTONE H3 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451:SF0">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE, H3 LYSINE-79 SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08123">
        <property type="entry name" value="DOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51569">
        <property type="entry name" value="DOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DOT1" evidence="14">
        <location>
            <begin position="667"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="350"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="389"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="998"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="248"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="389"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="402"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="432"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="999"/>
            <end position="1030"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU271113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW31747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1046" mass="119856" checksum="EB20001366D4814A" modified="2011-11-16" version="1">MSSCNSSLGQLIPRYTSCVNSVTAKKCDKSDLELLHSMYTYDLTRTGLSNALPNMTIEECKVLVQKFADYLYFTPKETEYLTNNLDKPVEELTHELPLRTQHSITCKIRELSKRISKVKVESSELKHGPLTEDEREYLQTCINNDLTLSGLEETFTNRPIGDIIRLIKEENDSIPFTSGEKELLKANIKNKISVEECLDDFPIRDEEYVRDRYKEYDFVSTRVTHFNTPEERLVYEARWAMMMTGDSTSRRASRKRKLEEDLEEMQRQSAIVVKPVKVELTPEDIERRKVHALKIQESRERKRLEALQKRLEYQARKAAGLIPQKPRSEDSKHYGLKALVSSSEYFQSVTGDKKKVEEGQKRRRIQAEHYTPELHIKKKVVRLKTTARQAEKKKLKQELREKQKKKKLTEKGKQRKKPGPKPKKKKEVKEEYIEEEIEDDDPTSEPEEEEEDFISPYDPIDINSDTLIPLNGRQLYVPSFYDEKPSVPKLNFVNVPEDSVTSDDMTPSKKVMTDLNDDILLVDNLAAEIVATHNKQYRDLPISFPPFLSPLGKINPLNEIKVRFLLYPQHCESFILAEPKSNELDPIYEIVKLFMIHYSLYFSHSDVLREIITEDYCHKLENSVEENDFCEFMNVIDKWNTLMLKLSPNSNAVEEILSNNKDINAGPRSYLVSQEIKTVINDEDLKLETFYGEIMLESVSPSFQLLRELSLDEKNEPRAEVGPGHAVVPINVSDEVKDLKPDNYEETLFKRFQEKTTVSRFTIQQILLRVYSRIVSTDSRKLRSYKAFTAEVYGELLPSFTSEVLEKVKLLPTQKFYDLGSGVGNTTFQAALEFGACLSGGCELMEHASKLTNLQTGLIQKHLAILGLPSLKLDFALSQSFVNNEAVRNVCLDCDVLIINNYLFDGELNAQVGRLLVGLRPGTKIISLRNFISPRYRATFDTVFDYFRVEKHEMSDVMSVSWTANKVPYYISTVEDNILPEYLSNDFERSVTPIVRSGSTTTNASDSVSASVSVSATVTPQQSPQHSPVPSNSPPTSPSALKFILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARG9</accession>
    <name>G3ARG9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Oleate-induced peroxisomal protein POX18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Lipid-transfer protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_61858</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Is involved in beta-oxidation of long-chain fatty acids. Its exact function is unknown, but possesses a nonspecific lipid-transfer activity, despite the absence of a cysteine residue thought to be essential for the activity of its mammalian counterparts.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376068.1">
        <property type="nucleotide sequence ID" value="XM_007376006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARG9"/>
    <dbReference type="STRING" id="619300.G3ARG9"/>
    <dbReference type="GeneID" id="18874083"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61858"/>
    <dbReference type="eggNOG" id="ENOG502S8FH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105945_0_2_1"/>
    <dbReference type="InParanoid" id="G3ARG9"/>
    <dbReference type="OMA" id="DLPMAMK"/>
    <dbReference type="OrthoDB" id="10265837at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1050.10:FF:000001">
        <property type="entry name" value="Putative Non-specific lipid-transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="entry name" value="SCP2 sterol-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094:SF25">
        <property type="entry name" value="SCP2 STEROL-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094">
        <property type="entry name" value="STEROL CARRIER PROTEIN 2 SCP-2 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP2" evidence="9">
        <location>
            <begin position="21"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00078512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="130" mass="14201" checksum="32B884E1513247F8" modified="2011-11-16" version="1">MSSVAADGFNASELWELLEAALHDKEIAETAIKNVKAILVITLKNKEGKELSWVLDLKNEGTLKKVEGPPPKGDVQLILKDADFVKLANGKANGQKLFMNGKLKIRGNMMKATALEGVFKSMDPRPKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS26</accession>
    <name>G3AS26_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nucleoporin Nup159/Nup146 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_51842</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376653.1">
        <property type="nucleotide sequence ID" value="XM_007376591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS26"/>
    <dbReference type="STRING" id="619300.G3AS26"/>
    <dbReference type="GeneID" id="18871600"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51842"/>
    <dbReference type="eggNOG" id="KOG3630">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011298_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS26"/>
    <dbReference type="OMA" id="VEMFAIN"/>
    <dbReference type="OrthoDB" id="248320at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039462">
        <property type="entry name" value="Nup159/Nup146_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16755">
        <property type="entry name" value="Beta-prop_NUP159_NUP214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117289">
        <property type="entry name" value="Nucleoporin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup159/Nup146 N-terminal" evidence="6">
        <location>
            <begin position="77"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="390"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="500"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="575"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="747"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1040"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="401"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="425"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="671"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="685"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="706"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="757"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1103" mass="120624" checksum="1B2B60D32F5FB4CA" modified="2011-11-16" version="1">MLVEEIISQDLGFKLDTPEHEIPLFDTPIDLSAHGSQDLNLLAVNNDLRLLAVSNISQLKLVSLDKLDKSTSIGDALSITQVHFNSSRLLVVNTSRIQTLSVSSYQIGDVAHLEYYPHLNKDIKSILPSPYTDSFLSLTVDNELYLCDDKSSTLIASNVSSYCWKQDGSYIYALNNDTTTLAGKSIEFDDPPSEDSHIVSMFPFNQYLLVTYDITSDDFEHDIKSYVFEDKPDYYIPMDVDTAPPFGMVERTATYYTSTINEWVESYSYSFITSSLATELSILSLSGHTLELIGYSEDIYRAQFPMNEETDDDCSPVGIGISLELSKEVAEPCPGVDSVTGKLPKVYILLDTGKLISYWVFHKHGLINDEVSLQRVIDYEKKLKSNAKPVASAPSIKPVSNEPSKGNEATTVQPENPFGSAENPFGSSGANPFGSSSANAFKPSTTSPATGFGGFGQKSDNFGSTGFGQAKPEGFGAAATSGAFGGSNFGKSLTGGAFGNQTKTSDTASPFGNQTTTIPGKQNTTNPLSGPTTFGSTSFGQPSSFTTHGFDNMTKTPQAAKNTAHSNFAKFSSSAASFGSGKTDIFSQSTDSPFGKLNEKPASSPFGKLNEQAGNSPFGKLNEQSTNSPFGKLNEQPANSPFAKLTKEEGPSDIFAQKDAPKEDAKKSAFGNFSGSKSIFEQPSQEDKPIESKPKSVFNPVSFAPETKKDQPTFESSEKLSSPGFSNFGKLSLASEEVKSLASPFAKLTQNAPVETQSEDVSDFSEETEEEEEISEEGESEELSEEGESEEIGQSIDLISQMKLKSESVIELPKKQPTIPSKSKLALSNDVLSTSEEEAETPEEINAPVEPIEFLVFDGLTRPTPKQGNVIGDEISRLVNITQGNLSVLKANIQLLGQFIQDHDSPTSADLSDVQRISQRDLLTPTIEKLVDPLHVLKSDLSKQLGQVDELSTQVQKCLYEKVKLDKLYSRLALLDTDNTKSHALKHRPLDIQNELMQMNLREKLAHVKTLESKLYSLIMPIKAVNSLNSETLNNIEQVVFQINSQIQNQRESIDKLEEEIKKLEVDDNKVELPQLTSSPRLKLVQRLRRTQPSSTAVKFMNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASF3</accession>
    <name>G3ASF3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_62998</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377103.1">
        <property type="nucleotide sequence ID" value="XM_007377041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASF3"/>
    <dbReference type="FunCoup" id="G3ASF3">
        <property type="interactions" value="395"/>
    </dbReference>
    <dbReference type="GeneID" id="18874546"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62998"/>
    <dbReference type="eggNOG" id="KOG2551">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051938_2_0_1"/>
    <dbReference type="InParanoid" id="G3ASF3"/>
    <dbReference type="OMA" id="EEPRGWW"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005645">
        <property type="entry name" value="FSH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050593">
        <property type="entry name" value="LovG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070:SF6">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03959">
        <property type="entry name" value="FSH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine hydrolase" evidence="3">
        <location>
            <begin position="9"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="254"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="254"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="291" mass="33371" checksum="5FEE9386678F499E" modified="2011-11-16" version="1">MSQGKKQYKGKILFLHGYTQSASLFYAKTSALRKKLIKLGYKSVYLNGPYVLTPAQLPSTDSLSKFSSAANEDMKYRAWWVKRDSTNDRISLEESIESIRNYIDKGEIIADSDDVDESDAESEEEKKLPIVGIIGFSQGAALGGLVAHKFDEIFKVDPLKFVILYSGFKLNTTKKSGNDRYDHYYPEEQDVERINLKYLHIYGELDTVVEESRALSLYELTKRRSDLLRHPGGHFVPNSKLYIDQVTNWIHKVEQEDKEEDKEKNEVKNGEDKDKHDLATLMDMMDSFGTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASG1</accession>
    <name>G3ASG1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Extracellular membrane protein CFEM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63006</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377112.1">
        <property type="nucleotide sequence ID" value="XM_007377050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASG1"/>
    <dbReference type="GeneID" id="18874549"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63006"/>
    <dbReference type="HOGENOM" id="CLU_2365409_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASG1"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442682" description="Extracellular membrane protein CFEM domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="96" mass="11323" checksum="69D2A0BB404B5448" modified="2011-11-16" version="1" fragment="single">MRLLIIYFISIISGTKLFERDDDCIEICTKAHALIKPCYDLAHDHIEFSKCICEDEKYRKALQECAQNCADDTDYESEQEDYEESCRDFMKNYSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASI5</accession>
    <name>G3ASI5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_72958</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="2"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377136.1">
        <property type="nucleotide sequence ID" value="XM_007377074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASI5"/>
    <dbReference type="FunCoup" id="G3ASI5">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASI5"/>
    <dbReference type="GeneID" id="18875462"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72958"/>
    <dbReference type="eggNOG" id="KOG0941">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002173_5_1_1"/>
    <dbReference type="InParanoid" id="G3ASI5"/>
    <dbReference type="OMA" id="MYYLFGA"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044611">
        <property type="entry name" value="E3A/B/C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01053">
        <property type="entry name" value="LSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700:SF8">
        <property type="entry name" value="HECT-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="6">
        <location>
            <begin position="511"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="5">
        <location>
            <position position="813"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="845" mass="96847" checksum="0C22DA470E6594D9" modified="2011-11-16" version="1">MLARDQHQRRHRNLNCYSHDLSKCYCCGTVLTFPSRAKKFRCSVCQTTNLFELQTSSFPTSSETDLPHVVSYEYVKKLVDRCYNSTSSESGQSQTLHQIFEPLSSYLYLAFKSNDVLSKSFKVRKASKRAHYHTSNINYQDIHDMFSLLTKLPTKRPLFSALKGASEQLKRILIFKDSNDPRNYYWLLILLEIPILQRSLLDNDASTSKLMSDVPEIKMLSYDILKRCLGILSCIDAITTTNYITSWFSKMDDDQFVKNVDLINLYITFQLKKYLYLANNPGVRLSPNPQHSPNEHVSSHDAEYFQNLNLKRHLEVDDPVEAHLSVTSPPANLFGKPKGKKKEKGEVKVKVHQYGNDWHIKSALMVMNMLYRANISRLHPIPVSSFYNSLVDFVNIKLDYDSWQSSKRNSLREGKSTHPEISQVIGFLHGTSINKNLFDDALYYFCQYPFLISLGGKISVLEYEARRQMGRKAEEAFINSLDKRTTIDIYFKVKVRRERIVQDSLECIKLNSNNLKKSLKVHFINEPGVDAGGLRKEWFLLLTRAIFHPQTGMLHNVEDSNYLWFNIVPVENFEMYYLFGAVLGLAIYNSTILDLQFPLALYKILMGKSLDHNDYKTIFPVSYKNLMSLKTMTAEELAELELTFEVSYSDAFGTNLTGDLIPTGSQISVTADNLDEYIEKYIGFFIKDGIKRQVRSFHEGFIHVIGGNALSLFSPEEIELLLCGSDDSGIDVDLLKSVTKYSGWKPSEDAQNSNIIKWFWEYMNDMSNKERRKLLGFVTGSDRVPATGIQNLVFKITLLKAYDSNRLPVAHTCFNELAIYEYSSKAKLVGKLSKAIFESAGFGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASJ1</accession>
    <name>G3ASJ1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_63036</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377142.1">
        <property type="nucleotide sequence ID" value="XM_007377080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASJ1"/>
    <dbReference type="STRING" id="619300.G3ASJ1"/>
    <dbReference type="GeneID" id="18874563"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63036"/>
    <dbReference type="eggNOG" id="ENOG502S656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057740_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASJ1"/>
    <dbReference type="OrthoDB" id="4977at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028133">
        <property type="entry name" value="Dynamitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15346">
        <property type="entry name" value="DYNACTIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04912">
        <property type="entry name" value="Dynamitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="263" mass="29701" checksum="6E2BA9D31AB4CDD9" modified="2011-11-16" version="1" fragment="single">MLDISPGQEIYETSDVESEDLPQKSITTTDTSEEVYTDSLEPEASRKQFSKNILSTNADVDFSGSITSSLRTSGYIVSRVNETVAEKLSRIARELEEIKNEDVDSAQLNELIEKYHELRGNREARKQCQFQGLNLDSVFEEVAAKDKEESSDTSLKEITELESRVNTLETIVGSEAILEHEARPEKSIQQTLNDLERKISIINNPEYSFDAIKQQISELVQEAEKLELSKKLMLNLAPAAVQPEKQKESNKKIDELYKALPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASR1</accession>
    <name>G3ASR1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_52296</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377158.1">
        <property type="nucleotide sequence ID" value="XM_007377096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASR1"/>
    <dbReference type="FunCoup" id="G3ASR1">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASR1"/>
    <dbReference type="GeneID" id="18871658"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52296"/>
    <dbReference type="eggNOG" id="KOG4372">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007367_1_0_1"/>
    <dbReference type="InParanoid" id="G3ASR1"/>
    <dbReference type="OMA" id="CFITADQ"/>
    <dbReference type="OrthoDB" id="5368485at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00741">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016445">
        <property type="entry name" value="Rog1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF62">
        <property type="entry name" value="LIPASE ROG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005412">
        <property type="entry name" value="UCP005412_abhydr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="3">
        <location>
            <begin position="211"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="770" mass="86445" checksum="B275D23EB2FAEE5B" modified="2011-11-16" version="1">MTEEENGDRLPYSQIPIALTKDQLDKMEDRILYRQVKSVKAGQIERFKINFTPRLDGDSVAIPPTLWVKVRNREPVPKRAVFLAGPYILYVDCRSTDYNPNQKCFITADQPVYEPQLLPGQSFYIQLSCHTLKPDYSWNIDVISQIIFNNTISIDFEIIIGTSKQILHEASYPERHITNSDKTGNFSPYLNIDHWDTFDLWNLPVPKQDRPIHLVILTHGLHSNASVDMLFLKERIDAGADPNSNIVVKAFFGNIGKTERGVKYLGSRVAEYIIELITANETFNNGKVDKISFIGHSLGGCVQTFAIAYLKVNFPWFFEQIKPINFVTLASPLLGVANENPAYVNLVLSAGFVGKTGQELGLKYFEKDSKPLLLLLPAGPTHTVLKSFKRRTVYANAINDGIVPLRTSSLLYLDYKTISHIINSEKEKVAVSEGGGRKIMKNIDISSVQTMLSYFMPQRESVYHKFQTSDEVAGEVTDSGVDDSITSDENNDEIGEIPPTTFMDSAASVLLPPLPPAKYIIDPDSRENPIIHDKVYYKKDIPRPKSPATSIEMAEEDSYSGIIKKKILSDYSKFEEAIAQEYHRDMGWRKVIVKLKPDAHNNMFVRRRFANAYGWPVIQHLVDNHFGPNRTPDMNIVSGNSVSSSDELASEDLTRIVSRDLITRQNIEIDESVTEEEHAWINSKDNSESLFAVGVTGLLGEVTEMMGDFREQIYKLGLNPSFAQLSQAISIVPPTANANQTTVNNESQSDVEISESGDLGVKQGIMGDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AST8</accession>
    <name>G3AST8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Succinate dehydrogenase [ubiquinone] cytochrome b small subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_63063</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the CybS family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377185.1">
        <property type="nucleotide sequence ID" value="XM_007377123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AST8"/>
    <dbReference type="FunCoup" id="G3AST8">
        <property type="interactions" value="254"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AST8"/>
    <dbReference type="GeneID" id="18874579"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63063"/>
    <dbReference type="eggNOG" id="KOG4097">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096618_0_0_1"/>
    <dbReference type="InParanoid" id="G3AST8"/>
    <dbReference type="OMA" id="DYIPKRK"/>
    <dbReference type="OrthoDB" id="18577at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046685">
        <property type="term" value="P:response to arsenic-containing substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006970">
        <property type="term" value="P:response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03496">
        <property type="entry name" value="SQR_TypeC_CybS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1300.10:FF:000007">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] cytochrome b small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007992">
        <property type="entry name" value="CybS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13337">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13337:SF2">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] CYTOCHROME B SMALL SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05328">
        <property type="entry name" value="CybS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <note>ligand shared with SDHC</note>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>a ubiquinone</name>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <note>ligand shared with IP/SDHB</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR607992-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR607992-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="160" mass="18131" checksum="13967B5F299AB81E" modified="2011-11-16" version="1">MLARQSIITTKPTHLILQRQFRLIPDFSKFKKIEQPPGYIVGTVNDAYIPPPTKHIDGSYHWTYDRVVTIGMIPLMMTPFIAGIEFPMVDSALSTLLLWHCHAGFKSCIIDYIPERVYGIWHKMATGLLTAGSCLGMYGIYVMETANNGLFELLSNIWHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATF9</accession>
    <name>G3ATF9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62836</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376955.1">
        <property type="nucleotide sequence ID" value="XM_007376893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATF9"/>
    <dbReference type="GeneID" id="18874486"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62836"/>
    <dbReference type="HOGENOM" id="CLU_2849478_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATF9"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="61" mass="7022" checksum="3E932BA554E325FE" modified="2011-11-16" version="1">MNPTLRLAYNKLRPTPIYVYYGRRVLPVAGWFSGMIIMLGWPFVWSSTSNKLHNVPNINGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATH1</accession>
    <name>G3ATH1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4</fullName>
            <ecNumber evidence="6">3.1.13.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Carbon catabolite repressor protein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Cytoplasmic deadenylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Glucose-repressible alcohol dehydrogenase transcriptional effector</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">SPAPADRAFT_68158</name>
    </gene>
    <organism evidence="26">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CCR4/nocturin family.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="6"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376967.1">
        <property type="nucleotide sequence ID" value="XM_007376905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATH1"/>
    <dbReference type="FunCoup" id="G3ATH1">
        <property type="interactions" value="468"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATH1"/>
    <dbReference type="GeneID" id="18875200"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68158"/>
    <dbReference type="eggNOG" id="KOG0620">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016428_4_1_1"/>
    <dbReference type="InParanoid" id="G3ATH1"/>
    <dbReference type="OMA" id="LWHAIDF"/>
    <dbReference type="OrthoDB" id="428734at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007089">
        <property type="term" value="P:traversing start control point of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09097">
        <property type="entry name" value="Deadenylase_CCR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000037">
        <property type="entry name" value="Glucose-repressible alcohol dehydrogenase transcriptional effector"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF100">
        <property type="entry name" value="POLY(A)-SPECIFIC RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="17" id="KW-0804">Transcription</keyword>
    <keyword evidence="16" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="24">
        <location>
            <begin position="475"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="39"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="104"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="261"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="798"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="264"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00030493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00031469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00033317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="EGW30934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="817" mass="94214" checksum="D6C8AE7A7996C459" modified="2011-11-16" version="1">MNVNNKYQHAAAQIQGQPQTNLQAQQILLQQLHQGQSQQQQQQQQQQQQQQQPGANALGAGFQPNENFNDTLNQGIYQANYQRAQPSQQQLQQQFFPQFQQGQQAQGNAQQLQQPYQPQQLQQQQQQQYQPNQQQQQQQVPRQPPHLQQQQFYTQQQQQQQAALQAQQQVQLQQVQQQVQQQQQQTPVQKLSTINIDNPNSLYWQHQQQLCQLSRTSNIPHSYARQYASNSRKNKNPYNEVKSVGLIEATKTIIASLEEQEKANNSNNQQSTPTSNTALLHNKKHSQDLDDDTMEEQRMRLKTQGKQLWCQLDLSGQGLVNISPKLFHYDFLESLYLSNNKLTSIPPVISKLRSLRTLDLSHNRISEIPSELGLCFNLRYLYLFDNNIKTLPNSFGNLIELLFLGIEGNPMDLSIANLLAEKGTKELITTLRDQKITPIRTPKPRPWLLLEDDGEIADSVETFRNDTNGDSFTLLSYNTLCQHYATPRMYKFTPSWALDWDYRRNALQREILGYNTDIICMQEVETRTFNEFWLPLMSEHGYRGVFFCKTRAKTMSEADAKKVDGCATFFKAEKFNLVQKQNFEYNSVCMGSDKYKKTKDLFNRFMNKDNTALITFLQHKESGEHMTIVNTHLHWDPSFNDVKTLQVGILLEEMQGIIKKFLHTSSMEEVKNATMIVCGDFNSVKESAVYQLFSTGASVNHEDMAGRDYGKFTEEGFRNAFKLKSAYETLGELPFTNLTPAFTDNIDYIWYSTNSLQVRGLLDKVDMDYASHRIGFPDANFPSDHIPILAKFQFKKGSAAKKPDFKPDFKTGPSRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATL4</accession>
    <name>G3ATL4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62880</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377010.1">
        <property type="nucleotide sequence ID" value="XM_007376948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATL4"/>
    <dbReference type="GeneID" id="18874507"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62880"/>
    <dbReference type="eggNOG" id="KOG1144">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2256570_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATL4"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="104" mass="11250" checksum="ACE52E71A06C9332" modified="2011-11-16" version="1" fragment="single">MAKKGKKGGKSGGDFWDDEDMAQEQPQAEEFGNGAESAEPVEPAESAEATPEPAGPQEASADDIAGDFLSSIRKNKQKKDQKEEDDKKSKDGPKILSKKEKEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU60</accession>
    <name>G3AU60_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_157463</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377407.1">
        <property type="nucleotide sequence ID" value="XM_007377345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU60"/>
    <dbReference type="GeneID" id="18871082"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157463"/>
    <dbReference type="eggNOG" id="KOG1208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_6_1"/>
    <dbReference type="InParanoid" id="G3AU60"/>
    <dbReference type="OMA" id="INWENPT"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF236">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="334" mass="37717" checksum="BFCAFE085BBF1F27" modified="2011-11-16" version="1">MNPKIPSKTCADWKQLFQGQYPGKPSFTEDKYPDLKGKVVIVTGGNSGVGYQTVKSLAGSTEARIYIFSRNEQKSLDAIAQIRKEVAQEYKVFNPDIHFVKVDLSDLTTVKPAVEEFCKRETRLDIIIHNAGVLMPPTGSKSAQGYELQLGTNVIGPHLLQRLLDPIFIKTSKTNKPGESRIVWVSSSFHFFAPQGGFFWDDLNYEQVKSMFLSRYYAVTLYGQSKAGTVIQARTWSLKHNAPNVISSSVCPGYLKTGLQKDCTMFEKVVFWLTLHPARYGAYTELFAALSPDVKNRSHSIAYGRQSHVRKDLMDEKTCDKVWKYLDKVTDTYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU97</accession>
    <name>G3AU97_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">rRNA methyltransferase 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_157554</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377444.1">
        <property type="nucleotide sequence ID" value="XM_007377382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU97"/>
    <dbReference type="STRING" id="619300.G3AU97"/>
    <dbReference type="GeneID" id="18871087"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157554"/>
    <dbReference type="eggNOG" id="KOG4589">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_776378_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU97"/>
    <dbReference type="OMA" id="FKEISWT"/>
    <dbReference type="OrthoDB" id="20105at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF18">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="7">
        <location>
            <begin position="76"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="354" mass="41382" checksum="5B0CA3ACA8751FE1" modified="2011-11-16" version="1">MLSLIRHRLVRNISPVVILRQFSTCRPLLLAHSADSDVEFIHIPKPHIPEYNRNANKRHKNAIKRVLDNEYAYNSLETIDMEERIFDKSVTKVVDTGYVPGNWMRYTLERLSELHGVDEEKLYKKCYVLGVDLLFQTPPNGVSTIQGNIFSKLTHKSVITQLRQWTELNKQQKRKKDNDEEIDPKSYITKEQEELKLEHEITGIENKLENLSLDEYRADVVLSDLARPFMQSTGYYNNSMNTPYLRTYQNEPLKQVLTKPDKACIDLADAALMLACRALRPGGTLVLRLETVRTGDPETDLLRRRLDMVFNHVHIRRTDNSRGFSRDGDHELYYICRNKRKDGEYDIRTIFNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUC0</accession>
    <name>G3AUC0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_143507</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377467.1">
        <property type="nucleotide sequence ID" value="XM_007377405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUC0"/>
    <dbReference type="FunCoup" id="G3AUC0">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUC0"/>
    <dbReference type="GeneID" id="18870550"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143507"/>
    <dbReference type="eggNOG" id="KOG3780">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008578_0_1_1"/>
    <dbReference type="InParanoid" id="G3AUC0"/>
    <dbReference type="OMA" id="FHILSCK"/>
    <dbReference type="OrthoDB" id="2238745at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF174">
        <property type="entry name" value="ARRESTIN-RELATED TRAFFICKING ADAPTER 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="315"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="606" mass="67764" checksum="C31C2722DB39E1D8" modified="2011-11-16" version="1" fragment="single">MRPNSPIFPPQQLSINSPMSQLDQDLDSPGARPASPALLPPLPDKPTCSTSSIQVYIIPTEQNLFVEGFDAQEYGSRPPTLLRGCLYVRVLKPTKIKSITLSFKGVQRTEWPEGIPPKKQLYAEINDVISHTWPFFTGTNNTINNGADYFVELNNHRRYLSASFNDLKRLNTNSSDSSLSSASVADTTASSTVVNFLSRNLSPSFIRRGKSPSVSSMDMPDLSSVISPGSVNDANGPTTFAPGDYIYNFEHPLPPSIPETTNVTFGSTNYNMEVSIIRAGAFKSNLSGKLPINIVRTPAESNMEENESIVITRDWEDQLRYDIVIGSKSIVLDSYLPLAFRFVPLWGKVSLHRIRVYLSENLEYFCSNKRVHRMEPPKKFLLLEHKAKKGRSLLSSRPVNDDDDAEDEEEDNFDEDILPKELEFQLYVPHDLNNRYSAKIHPDTSYENIQAHHWIKICLRISKPDPNNPEKRKHYEISIDSPMHVLSPLAAHTNTLLPAYDDFIPQLSSEPLSPGVIPVDGTATPEIEFHHISTTTDLDGVARDADMHLSANLYHPTPDEATIRAMNSPQAVPHPNIYSSPLVRPIHILRKPSVNPPPFDADISPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUV1</accession>
    <name>G3AUV1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Gylcosyl hydrolase 115 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_158400</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377808.1">
        <property type="nucleotide sequence ID" value="XM_007377746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUV1"/>
    <dbReference type="GeneID" id="18871126"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_158400"/>
    <dbReference type="eggNOG" id="ENOG502QTU7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004852_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUV1"/>
    <dbReference type="OMA" id="GPRNYKW"/>
    <dbReference type="OrthoDB" id="4849794at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.379.10">
        <property type="entry name" value="Chitobiase/beta-hexosaminidase domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.520">
        <property type="entry name" value="Glycosyl hydrolase family 115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031924">
        <property type="entry name" value="GH115"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041437">
        <property type="entry name" value="GH115_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042301">
        <property type="entry name" value="GH115_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029018">
        <property type="entry name" value="Hex-like_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37842">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37842:SF2">
        <property type="entry name" value="GYLCOSYL HYDROLASE 115 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17829">
        <property type="entry name" value="GH115_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15979">
        <property type="entry name" value="Glyco_hydro_115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442638" description="Gylcosyl hydrolase 115 C-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="domain" description="Gylcosyl hydrolase 115 C-terminal" evidence="3">
        <location>
            <begin position="820"/>
            <end position="995"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1000" mass="113181" checksum="F72FA3FDF71DA1FF" modified="2011-11-16" version="1">MLFSYIPTLIALLGAVNALGGQTNIAFQQKGSHGNNKEITLHDHSSTATLFLDQNDWPGVNRAAEDLSDDFRKVTGHALPVSNYTSSNDICKKIHGSVENAIIIGTIGNNTLIQNLIKEKKINVDKIKGKWESYIQEQVENPVPCIKNALVIAGSDKRGAIFGAYDISEQIGVSPWYYFADVVPQSHDSIYFSGSKTQGEPGVKYRGIFINDEQPAIAAWVAEFFPEGKYNSYFVHQFYVKLFECLLRMRANYLWPAMWASMFGVDDPENQYWADYYGVVMATSHTEPLMRATNEWSTFGKGAWDYSTNKDNIVSFWKDGIERSKPFENVWTMGIRGFGDTPISGGVETSLLEDVIATQRELLGQYFGNSSVTDIPQSWCLYKEVQSYYEAGMPVPDDITLLWVDDNWGNIRRLPLNNETSRSGGAGVYYHFDYVGDPVDYKWINTISNEKTWEQMHLAKERGADRIWLVNVGDIKPLEVPIEYFISLGYHYDVWGPINKVFEYTEAWAQREFGQWYKNTTEIADIVDLYGFLINRKKYESLNATTYAIYNYQEADTVLQQWSDLSARAWNVYNQLPKNVQPSFFQLVLHPVNAGYIVHDIMISAGKNNVYAEQRRNQANSLATYVQTRFGDDHSWKNQWDSMLNGKWHHMMDQTHLGYFFWQQPMRDVMPPVSICQADDNNLAGAMGVTVDQSRGSVPGDDLYNAVAYSNNTLITPPQDPYTGNTFIEIFNRGNEDVNFKVSSENDFVTLSQTTGYISATNDSIWNSVKVWVEVDWSKAPEGSNIVFIDVTSNTTYGFFGPPQIHVPINKNQAPTNFKGFIEAGEVISIEAEHFSKKVDNNKVNYEVINRYGRTLSGVTLFPVTADSQTSDANAPHLEYDFYSFSTPAYGTNITVYTSPSLNTYPARPLKYAIAIDDEQPQEVQLVIDTTNPLNMPDHWEQAASDTIWLHNTTHNISSSGQHTLKLWALEPGVVFQKAVIDFGGVVPSFLGPPETYRQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUW9</accession>
    <name>G3AUW9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_63678</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377826.1">
        <property type="nucleotide sequence ID" value="XM_007377764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUW9"/>
    <dbReference type="STRING" id="619300.G3AUW9"/>
    <dbReference type="GeneID" id="18874864"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63678"/>
    <dbReference type="eggNOG" id="KOG1762">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114656_1_0_1"/>
    <dbReference type="InParanoid" id="G3AUW9"/>
    <dbReference type="OMA" id="NVWADVY"/>
    <dbReference type="OrthoDB" id="2194681at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05831">
        <property type="entry name" value="Ribosomal_P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696:SF33">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="74"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="85"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="107" mass="10752" checksum="8CD68BACCE4100F6" modified="2011-11-16" version="1">MSAEASVSYAALILADAEQEITAEKLLAITNAAGASVEGVWANVFAKAVEGKNLKELLFSFAASAPAASGAAPAAGAAGAAADEPAAEEKEEEKEESDDDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV97</accession>
    <name>G3AV97_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">asparagine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Asparaginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_144610</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Asn) + L-asparagine + ATP = L-asparaginyl-tRNA(Asn) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11180"/>
            <dbReference type="Rhea" id="RHEA-COMP:9659"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="3"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377666.1">
        <property type="nucleotide sequence ID" value="XM_007377604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV97"/>
    <dbReference type="FunCoup" id="G3AV97">
        <property type="interactions" value="933"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV97"/>
    <dbReference type="GeneID" id="18870648"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144610"/>
    <dbReference type="eggNOG" id="KOG0555">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004553_2_10_1"/>
    <dbReference type="InParanoid" id="G3AV97"/>
    <dbReference type="OMA" id="DCCLYPR"/>
    <dbReference type="OrthoDB" id="1931232at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04323">
        <property type="entry name" value="AsnRS_cyto_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000040">
        <property type="entry name" value="Asparagine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1910.20">
        <property type="entry name" value="asparaginyl-tRNA synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048952">
        <property type="entry name" value="AsnRS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF16">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20917">
        <property type="entry name" value="AsnRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="14">
        <location>
            <begin position="249"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="59"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW29900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="552" mass="61950" checksum="B794D703E19CCDC2" modified="2011-11-16" version="1">MSQVHVNEKTGVDSAEAKGTEQQPFATAAYALYVTPGAKVLVYKQSEEDETFSYVEISASALKKAVKGAEGLKKKAEKQAKLEEQRKLKEQEATAKLAELDLIKIEEDPSLPAAKKIKLRTIQDHLETRVLVQGWIHRLRVQKGVAFITLRDGTGFIQAVLSGDLAKARQTQELTLESTVVIKGVISKLPEGKTAPGGVELKADYYEIVGLAPSGDDAFTNKVQENADPSLLLDQRHLALRGESLSAVMKVRAAFLGAVRRFFAEEGLLEVTPPCMVQTQVEGGSTLFKMDYYGEEAYLTQSSQLYLETCLPALGDVYCVQESFRAEKSHTRRHLSEYTHIESELAFIEFEDLLSHLERLITKTVQYVLEDPVTAALVKQLNPNFQPPKMPFLRMEYIQALDWLNEHGVLNEDGEKFKFGDDIAEAAERKMTDTINQPILLIRFPVEIKSFYMQKCKDDPRVTESVDVLIPNVGEITGGSMRTYDHDELLAGIKREGLDADAYYWFIDQRKYGTCPHGGYGLGTERILAWLCDRFTVRDCSLYPRFSGRCKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVC9</accession>
    <name>G3AVC9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD40 repeat-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_52975</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat MORG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377914.1">
        <property type="nucleotide sequence ID" value="XM_007377852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVC9"/>
    <dbReference type="GeneID" id="18871744"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52975"/>
    <dbReference type="eggNOG" id="ENOG502RS0S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_850240_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVC9"/>
    <dbReference type="OMA" id="IDCKFIN"/>
    <dbReference type="OrthoDB" id="1068471at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051980">
        <property type="entry name" value="WD_repeat_MORG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22842:SF3">
        <property type="entry name" value="WD REPEAT DOMAIN-CONTAINING PROTEIN 83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22842">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="329" mass="36541" checksum="FA54E555BA64E63D" modified="2011-11-16" version="1">MASKSWSYQAHEYPISTFCRVEGTNYILTVAKDEIKLKNLEDTQAQQTLFTKSHNADITSIDASPSTLKVVSASFDQVLLTDMTVGDVIMRFSGNKESKGKLVRDCKFLSRNLIATCGDYTALNLADIRERPASNSSKIQESPNCWTYPFSTEDNLTSLGYSHPYISMGSSSGTAYILDLRNSQFIYSNVSKNLLMAVEQHPGNLSVSFDKEGIIRFYDVKKDQFMAQGCCVNRAIEHQINMHYVPELDFVINGSEEGIVHLWRLNEDRSRFVSALNFAVLGRVDSPASGIPAANTINHVDFDPAAKRLIASSASGVLHVWDDLSNLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEH1</accession>
    <name>G3AEH1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein kinase</fullName>
            <ecNumber evidence="8">2.7.11.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_63966</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.21" evidence="8"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372145.1">
        <property type="nucleotide sequence ID" value="XM_007372083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEH1"/>
    <dbReference type="FunCoup" id="G3AEH1">
        <property type="interactions" value="342"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEH1"/>
    <dbReference type="GeneID" id="18874953"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63966"/>
    <dbReference type="eggNOG" id="KOG0575">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_46_2_1"/>
    <dbReference type="InParanoid" id="G3AEH1"/>
    <dbReference type="OMA" id="IQIHKSM"/>
    <dbReference type="OrthoDB" id="408964at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090619">
        <property type="term" value="C:meiotic spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035974">
        <property type="term" value="C:meiotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140602">
        <property type="term" value="C:nucleolar peripheral inclusion body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019237">
        <property type="term" value="F:centromeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051219">
        <property type="term" value="F:phosphoprotein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010458">
        <property type="term" value="P:exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031619">
        <property type="term" value="P:homologous chromosome orientation in meiotic metaphase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990571">
        <property type="term" value="P:meiotic centromere clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990813">
        <property type="term" value="P:meiotic centromeric cohesion protection in anaphase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090306">
        <property type="term" value="P:meiotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990395">
        <property type="term" value="P:meiotic spindle pole body organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904750">
        <property type="term" value="P:negative regulation of protein localization to nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045793">
        <property type="term" value="P:positive regulation of cell size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140281">
        <property type="term" value="P:positive regulation of mitotic division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140429">
        <property type="term" value="P:positive regulation of mitotic sister chromatid biorientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010696">
        <property type="term" value="P:positive regulation of mitotic spindle pole body separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046827">
        <property type="term" value="P:positive regulation of protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110083">
        <property type="term" value="P:positive regulation of protein localization to cell division site involved in mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031031">
        <property type="term" value="P:positive regulation of septation initiation signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903353">
        <property type="term" value="P:regulation of nucleus organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902542">
        <property type="term" value="P:regulation of protein localization to mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051455">
        <property type="term" value="P:spindle attachment to meiosis I kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070194">
        <property type="term" value="P:synaptonemal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13118">
        <property type="entry name" value="POLO_box_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13117">
        <property type="entry name" value="POLO_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14099">
        <property type="entry name" value="STKc_PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000647">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.30:FF:000005">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000091">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.30">
        <property type="entry name" value="POLO box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033701">
        <property type="entry name" value="POLO_box_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033695">
        <property type="entry name" value="POLO_box_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000959">
        <property type="entry name" value="POLO_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036947">
        <property type="entry name" value="POLO_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00659">
        <property type="entry name" value="POLO_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82615">
        <property type="entry name" value="Polo-box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50078">
        <property type="entry name" value="POLO_BOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="71"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="11">
        <location>
            <begin position="467"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="11">
        <location>
            <begin position="565"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="390"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW34733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="655" mass="74963" checksum="A06D5AC945234BB3" modified="2011-11-16" version="1">MSAFRAQPLQPLNSGQLNSRGTNITPIKTVRRPTENLPAEGAKQHKKKKEKLSALCKTPPSIVRTRNGRDYRRGNFLGEGGFARCFQMKDASGQIYAAKTVAKASIKNEKTKTKLLSEIKIHKSLKHPNIVNFVDCFEDDVNVYILLEICPNQSLMELLKARKRVSEPEVRFFMVQIVGAVKYLHSRRVIHRDLKLGNIFFDPDMNLKLGDFGLASVLPSTDSRKYTICGTPNYIAPEVLGGKHTGHSFEVDIWAIGIMMYALLVGKPPFQAKDVEVIYERIKKTEYYFPEDKPISPSAKTLIQDLLSLNPLNRPTIDEILNYEWFKGPFPDKTHEISLSETPVSLEQISRAQSSINFTNTKTNAGIYTPRNKDPVEILRSDLQSEQPRAMLPTSLSPNDTRNKYQEVDPSQMGRPRRVNFSANFSTAIKRLNQVCFETYQNMRRLEYAGKEQSQTQEAVDCENPTLISKWVDYSNKYGFSYQLNNDDIGVLFNDDNTLLKLHNSDRFLELLYHENEGWACVENSTSHPPTQAKRQLEIVDFFAKYMNSNLSKVSENEDRKEIVFLRRYTRTSDYIMFEMTNGNFQFNFKDHHKICISKSGMVITHISPNRSTRTLPLAAVLRQGNFPQEQVPSCLEKITVIKEAIKRKAFKEAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEN5</accession>
    <name>G3AEN5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58868</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373073.1">
        <property type="nucleotide sequence ID" value="XM_007373011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEN5"/>
    <dbReference type="GeneID" id="18872655"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58868"/>
    <dbReference type="HOGENOM" id="CLU_3088789_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEN5"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="52" mass="5616" checksum="01785D0CADDA78E3" modified="2011-11-16" version="1">MGIDSYALGVRSFPLFDNMMVTFSDGYTKGYLMAGKTGTIQPLVSCSKSIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEW7</accession>
    <name>G3AEW7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sister chromatid cohesion protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_147455</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SCC2/Nipped-B family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373209.1">
        <property type="nucleotide sequence ID" value="XM_007373147.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AEW7">
        <property type="interactions" value="233"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEW7"/>
    <dbReference type="GeneID" id="18870753"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_147455"/>
    <dbReference type="eggNOG" id="KOG1020">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004334_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEW7"/>
    <dbReference type="OMA" id="FNSRHVL"/>
    <dbReference type="OrthoDB" id="418242at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090694">
        <property type="term" value="C:Scc2-Scc4 cohesin loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061775">
        <property type="term" value="F:cohesin loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140588">
        <property type="term" value="P:chromatin looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034087">
        <property type="term" value="P:establishment of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071169">
        <property type="term" value="P:establishment of protein localization to chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024986">
        <property type="entry name" value="Nipped-B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033031">
        <property type="entry name" value="Scc2/Nipped-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704:SF18">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN DELANGIN SCC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12830">
        <property type="entry name" value="Nipped-B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20168">
        <property type="entry name" value="PDS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Sister chromatid cohesion C-terminal" evidence="3">
        <location>
            <begin position="1102"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="93"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="111"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU364107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1377" mass="157503" checksum="003B3AB0AFF703C1" modified="2011-11-16" version="1">MIIEEVMNFDDEELQSIRFERFTTIQQPSENVGPTVELKGLEAFMFNAQPINESFYQDPSVSAPPEWSKLSVRKKYDSTNKKVMDDHNQKYVTKFNNLRKQGTSKKAKRKRDPEQDGSEERNRKVIKFSELELHQIAETTVKTFVLEFPINESQEGKVNVPIEKLEEVSDSLTKILESTKDVDTQEVANLQRICHKLLSENIEKIKNDININSFDDIANSLVIFSQSAIATEVLLEILNSSIEDKSLFWDGYLDSAIEFVTLVFQDLLLPISQFSMQESAQMEPLKYNIGRLFREVSNLLRHIGNYNSKSQLEDQALTRIEILLTQVLFEEKYQHDKKSMLSLLPMDSIKISAGLVFVDIFRSCKDQRPYLLNELILNFPKLSTNKANFRQFKIERGFSVQYFTVILVKFIQSIESEDIKVKDSGFEDTISICDQIAVHLTTITADKFSPTLRTIFDFIVEDLLVLLPYPEWSAVEFMLTSLAKQFLSVLKNSETVLQIEVYFLEILGLICERVVSLEKLGKSESNDDIKEMLKDFNCIRFDRQNSLQSLRSRWLKSTVNEEIQASFQQALEFKFQSSDMNDINKVHPVYTSFILGSGLNLICMNFLDSLGQFLNSPKVKIRTKAVKILVNISEVKPNLFRSKKIQEALSARLLDASPLVRDAVFDFIGKYVQAHPNEADNFCAPLCNALGDTGISVRKRATKFAKDLYPKLTRRYNKVEVACKLLKRMDDEEDSVRNISISALIELLFLPLVSHSCTPEERVMTISVLVDICNSGTKVLSNLEVLLERHVLNSPEKGVQDAKKKLIELILEFVNDESMNEKVLFKSGALLLLASCVKSQPNIFGQDQLIALQPLITDEAQTIDPSYGYTLRVLRYSLVHINSLQPSFLVPVQNFLFKRLSKLTFQELDEAIPSLWKLCDMNSTKPRMINSVIATLKFLYPYIENKQRRHEKGLGKLLQLLGFFGKYCNFQEFYDSFKNSGIGLKNNESVVSLILKYIMQFCDDSTEIKNRIIAISTLITVCTSYTKLFGSEEVIKIFDREMDSNDTKIVMAILRGLIDFLNEKDADAQKKAGYEEKSSADNKLDVRVFHGISSVNAVDGVCAGMVQRYLNKVLKLCLSSESQVSYLPIQFVKRALELGYANPKMCIATIMALESSNNEYIRKTGIEMHQDIYEKHESIIESSYREGVSLALSSKQESEILVQGDFFNLLYRIVSGSVSSSKKFMKALGKGMSIISTKEKDPRKKLVYSVFYANNIANLEFKSMEELFIITSHLRSYLVGFTADTSEEENVENSSSRNQEWLSIMAMMKLYNHLTYKYHILDSHIDDYDSHASEIDLKQTPKVLRDVKIDLSWIVENVEGENPEIITKCKEELKQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF43</accession>
    <name>G3AF43_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_59024</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373231.1">
        <property type="nucleotide sequence ID" value="XM_007373169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF43"/>
    <dbReference type="STRING" id="619300.G3AF43"/>
    <dbReference type="GeneID" id="18872725"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59024"/>
    <dbReference type="eggNOG" id="KOG1558">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027089_0_1_1"/>
    <dbReference type="InParanoid" id="G3AF43"/>
    <dbReference type="OMA" id="QYTGCHS"/>
    <dbReference type="OrthoDB" id="448280at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF44">
        <property type="entry name" value="PROTEIN ZNTC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="275" mass="29688" checksum="BF2217159865CC12" modified="2011-11-16" version="1">MFNLDLEGMIITIIKQFGTGIIISTAFVHLMTHADLMWSNACLADKIHYEATGTALTMAGIFVAFVIEFIASRALKSRTAKTQQVQDTEVSRDSKEDQTSIVSSSPSLISLHGISSKDKISVVIMEAGIIFHSILIGITLVVAGDAYFITLFIVIVFHQFFEGLALGSRIVGLKNTALMTKLIMALVFALITPIGMAIGIGTLKTFNGNDPSTLIALATLDSFSAGVLLWTGLIEMWSQDWLHGYLSNAPITKTIFAMLALVAGLILMSLLGNWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF54</accession>
    <name>G3AF54_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dolichyldiphosphatase</fullName>
            <ecNumber evidence="6">3.6.1.43</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_130821</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for efficient N-glycosylation. Necessary for maintaining optimal levels of dolichol-linked oligosaccharides. Hydrolyzes dolichyl pyrophosphate at a very high rate and dolichyl monophosphate at a much lower rate. Does not act on phosphatidate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a di-trans,poly-cis-dolichyl diphosphate + H2O = a di-trans,poly-cis-dolichyl phosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14385"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19506"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57497"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="EC" id="3.6.1.43"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the dolichyldiphosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.43" evidence="6"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372255.1">
        <property type="nucleotide sequence ID" value="XM_007372193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF54"/>
    <dbReference type="FunCoup" id="G3AF54">
        <property type="interactions" value="364"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF54"/>
    <dbReference type="GeneID" id="18869532"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_130821"/>
    <dbReference type="eggNOG" id="KOG3146">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074922_0_1_1"/>
    <dbReference type="InParanoid" id="G3AF54"/>
    <dbReference type="OMA" id="LTVYQHE"/>
    <dbReference type="OrthoDB" id="302705at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047874">
        <property type="term" value="F:dolichyldiphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03382">
        <property type="entry name" value="PAP2_dolichyldiphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039667">
        <property type="entry name" value="Dolichyldiphosphatase_PAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247:SF1">
        <property type="entry name" value="DOLICHYLDIPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE/DOLICHYLDIPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="7">
        <location>
            <begin position="69"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="249" mass="29024" checksum="E1E8DCB31A567AE7" modified="2011-11-16" version="1">MLSFNSNSNLTTIYPAYQPVPFDHTYILYDPQDVISVICVQFSLLPIYIMVFYTAWFLITREIEPVIAVGGHLCNEIANKIVKRLIKHPRPDFHKDFGLGSYSLTYGMPSAHSQFMGFFAAYFIYTIHFKVDHMPARKKRFAYLLMITGMVAVAFSRIYLLYHTVPQVIVGVMVGFVLGICYFIASTLARDVGLVDWVLNWPIVKYFYIKDTYYYKYETFQQEHENYLAKRKSAKDTSIQKTTKEKELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFC0</accession>
    <name>G3AFC0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein necessary for structural stability of L-A double-stranded RNA-containing particles</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_131901</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372321.1">
        <property type="nucleotide sequence ID" value="XM_007372259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFC0"/>
    <dbReference type="FunCoup" id="G3AFC0">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFC0"/>
    <dbReference type="GeneID" id="18869618"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131901"/>
    <dbReference type="eggNOG" id="ENOG502RXN8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032834_1_0_1"/>
    <dbReference type="InParanoid" id="G3AFC0"/>
    <dbReference type="OMA" id="CNDIVET"/>
    <dbReference type="OrthoDB" id="497927at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47098">
        <property type="entry name" value="PROTEIN MAK32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47098:SF2">
        <property type="entry name" value="PROTEIN MAK32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="1">
        <location>
            <begin position="86"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="333" mass="37478" checksum="DDC7C62AAA440AB2" modified="2011-11-16" version="1">MSTILATLGMFIIDEIQYPPSWNKPPVLDQIGGAGPYAIMGARIIATSKYSNSISGIIDRGTDFPESIEQEIQSWSTGVIIRDCKDRLTTHGVNVYNEDEVRSFYYKSPKKQIEVDDVLQFEQLESSKCYHLICSISRCEEIIDRLNKHNQSREAKIFIYEPLPDDCKSENLDRLKRVLPKIHIFTPNLEEAGELLKSTNANYPDELAEEFNQWLQLPDSGTVIRCGKLGCHIRTKDGRTYDLPAYHQNQDNVVDVTGGGNSFCGGFIMGYYLSGGNWQVAGIAGNIASGCIIEQLGMPKVNSDLINRKSLSERLDIYKSQNPNFSEISFTWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFD7</accession>
    <name>G3AFD7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_132553</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372338.1">
        <property type="nucleotide sequence ID" value="XM_007372276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFD7"/>
    <dbReference type="FunCoup" id="G3AFD7">
        <property type="interactions" value="92"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFD7"/>
    <dbReference type="GeneID" id="18869668"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132553"/>
    <dbReference type="eggNOG" id="KOG4561">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034597_0_1_1"/>
    <dbReference type="InParanoid" id="G3AFD7"/>
    <dbReference type="OMA" id="MPVYYSH"/>
    <dbReference type="OrthoDB" id="10266980at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050846">
        <property type="entry name" value="TLCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439">
        <property type="entry name" value="CT120 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439:SF0">
        <property type="entry name" value="TOPOISOMERASE I DAMAGE AFFECTED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="70"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="291" mass="32969" checksum="19BC7F80067EC262" modified="2011-11-16" version="1">MSFITKYFPTDITEDPFAKYRPFPANPQSNLAAHWHEILASFLFYLIVQELSGPIFSRVMGPTYTKLPKRTKVNFDVHVASMVQCVVSFVLLVAHFNNEHFLNREQDPVNSLVGTTPFGCMVCSVTVGYFLWDIYVCTRYYRLFGLGFLFHGFAAAYAMGAGLLPYCQPWAGAFLTFELSTPFVNLNWFASKLPAGTFNERTIIINGLCLIISFFLVRIVWGFYAVYLLACDMLATWHLVPAFLPVSILALNFSLDTLNVFWLSKMIRIAIKKASGKESTRQAAHDAEKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFH1</accession>
    <name>G3AFH1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">3-prime end of extracellular mutant protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_58085</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SPS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372372.1">
        <property type="nucleotide sequence ID" value="XM_007372310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFH1"/>
    <dbReference type="FunCoup" id="G3AFH1">
        <property type="interactions" value="152"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFH1"/>
    <dbReference type="GeneID" id="18872313"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58085"/>
    <dbReference type="eggNOG" id="ENOG502QUZC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035846_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFH1"/>
    <dbReference type="OMA" id="DKFQCED"/>
    <dbReference type="OrthoDB" id="536881at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.20.20">
        <property type="entry name" value="Receptor L-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051648">
        <property type="entry name" value="CWI-Assembly_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036941">
        <property type="entry name" value="Rcpt_L-dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31018:SF3">
        <property type="entry name" value="RECEPTOR PROTEIN-TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31018">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442261" evidence="9">
        <location>
            <begin position="19"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="361"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="362"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="418" mass="43279" checksum="C9F15D04CB5C9060" modified="2011-11-16" version="1">MQFKKVFAVAAVAGVALAADKNNSTLTTATPSVTKDCSFSDFTATQSAQVAQVAACPTAVGDITIYGDAFGNIDLTGLEQVYGDLSIKNATKAAGVNAPTLQLVSGSLELNANTILSNLNLAQLTTVGTLNFNALPALETTGLTAGITSADSVIISDTGLSSLTGINVFELKTFNVNNNDQIETINSGLKRVTDTLDISYNAEKVDVTLDQLTGANNIILQSINSISAQNLTVANGSVAVSSSSVDKVQFKKLQTIGKSLTINKNDDLEELDFPKLTSIGGALEISDNDKLDSFDGFPQLKTIGGSVNMNGTFNNGTFESLQRVAGGFTLKTDGELSCQAFNKLNSNGDVKGDKFQCEDRVTTTSSSSKKNGSTETDSSDDSTGAAETTTSKKNDAPSPAGKLASVVAAFAAIGVALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFK6</accession>
    <name>G3AFK6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial outer membrane protein porin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_58118</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Forms a channel through the cell membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic mitochondrial porin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372407.1">
        <property type="nucleotide sequence ID" value="XM_007372345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFK6"/>
    <dbReference type="FunCoup" id="G3AFK6">
        <property type="interactions" value="1124"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFK6"/>
    <dbReference type="GeneID" id="18872330"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58118"/>
    <dbReference type="eggNOG" id="KOG3126">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044399_1_0_1"/>
    <dbReference type="InParanoid" id="G3AFK6"/>
    <dbReference type="OMA" id="FKQPAFH"/>
    <dbReference type="OrthoDB" id="7827681at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008308">
        <property type="term" value="F:voltage-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07306">
        <property type="entry name" value="Porin3_VDAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.10:FF:000004">
        <property type="entry name" value="Por1 mitochondrial outer membrane porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001925">
        <property type="entry name" value="Porin_Euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743:SF70">
        <property type="entry name" value="GH26960P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743">
        <property type="entry name" value="VOLTAGE-DEPENDENT ANION-SELECTIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00185">
        <property type="entry name" value="EUKARYTPORIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-0626">Porin</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="282" mass="29940" checksum="F2AAAA329D99B09D" modified="2011-11-16" version="1">MAPVAYSDLSKSSNDLINKDFYHLSTAAVDVKSVAPNGVTFTVKGKTTKDDTIAASVDAKYVDKATGLTLTQGWNNANALNTKFELSELLTPGLKGELETSVVPNGTRGAKLNFFYQQSAVNARLFFDLLKGPIATADLVVAHDGFTAGAELGYDISSAKVNKYSVGLGYANLDYSLAATATSNLSVFSAAYFHRVSPFVEVGAKTTWDSVKSSNVNVEFATKYALDRSSTIKAKIADSGLTALSYTQELRPGVKLGLGASFDALKLSEPVHKLGFSLSFTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFL0</accession>
    <name>G3AFL0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_53362</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372411.1">
        <property type="nucleotide sequence ID" value="XM_007372349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFL0"/>
    <dbReference type="FunCoup" id="G3AFL0">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFL0"/>
    <dbReference type="GeneID" id="18871778"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53362"/>
    <dbReference type="eggNOG" id="ENOG502SABN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048121_1_0_1"/>
    <dbReference type="InParanoid" id="G3AFL0"/>
    <dbReference type="OMA" id="NWRDKGT"/>
    <dbReference type="OrthoDB" id="272512at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063:SF60">
        <property type="entry name" value="LYSOPHOSPHATIDIC ACID:OLEOYL-COA ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063">
        <property type="entry name" value="PHOSPHOLIPID ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="290" mass="33419" checksum="8F81F7B7685C3B4C" modified="2011-11-16" version="1">MEKFSAWRDKATGISPFMPTKFPLVQEPSAVKRLGSYTLKTPIFIVKFPFFVLAYLLYTLTRWIGFAKFVIQLLFGLTTTFTVDGVKKSQTEKLEQYKPKAGDVLVTNYISPLDGFIVSVLANSSNTVFLIPNKQGTLYQYSALEVFNHCFSYEESKPVDDLSKLKNKIVVLLLEGTPSNNKSLLPFIKLDNKYNFDGFNVKSLVLKLSPSYFTLPIPYLGKFQYLFQLLTNLSKCSIKIKIYKFDKFDLNQIKNSFELNALTLINQELNLDSKQRFINYYFDHNIKKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFT2</accession>
    <name>G3AFT2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Clustered mitochondria protein homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Protein TIF31 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">CLU1</name>
        <name evidence="2" type="synonym">TIF31</name>
        <name evidence="5" type="ORF">SPAPADRAFT_132663</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">mRNA-binding protein involved in proper cytoplasmic distribution of mitochondria.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">May associate with the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLU family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372483.1">
        <property type="nucleotide sequence ID" value="XM_007372421.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AFT2">
        <property type="interactions" value="1040"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFT2"/>
    <dbReference type="GeneID" id="18869678"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132663"/>
    <dbReference type="eggNOG" id="KOG1839">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003256_2_0_1"/>
    <dbReference type="InParanoid" id="G3AFT2"/>
    <dbReference type="OMA" id="HPVWDKD"/>
    <dbReference type="OrthoDB" id="1414216at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15466">
        <property type="entry name" value="CLU-central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2280.10">
        <property type="entry name" value="Hypothetical protein (hspc210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03013">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033646">
        <property type="entry name" value="CLU-central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025697">
        <property type="entry name" value="CLU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028275">
        <property type="entry name" value="CLU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027523">
        <property type="entry name" value="CLU_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023231">
        <property type="entry name" value="GSKIP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601:SF6">
        <property type="entry name" value="CLUSTERED MITOCHONDRIA PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT EIF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13236">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15044">
        <property type="entry name" value="CLU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12807">
        <property type="entry name" value="eIF3_p135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103107">
        <property type="entry name" value="Hypothetical protein c14orf129, hspc210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51823">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Clu" evidence="4">
        <location>
            <begin position="344"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="745"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1255"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="745"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1259"/>
            <end position="1268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW35071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1304" mass="145404" checksum="ABCE08B57FEF2CAA" modified="2011-11-16" version="1">MSSGEPQAEVEEQQVAVKELALKVQLPHFLTTETLTLPSAYEESLADLKQSLSIIPLSRNLTSYTIVINGVDVTAHFDDLSTFAQIIEELGLADVEELNVEIKEKAYNLAAVYDQISRFREVIGLHYIDRISHDFGVAAGVSKFNNIELDDVKPKEDKAEQKPEQEEEAANEVQLPAEELTKIAEFAETVSMKSFSGKFVDATDFDKVNDALKVPIKSLSVSQWSPVPPFQKNKGDLLYLSLQTLEHETFNITCHFSGFFVNKSSTVNFNPEIKVNEKGKFFKSFLLYDLVSSLSPSFAKTIEENEINLSSATQHPETYLLPNNSYLAYPWLVNTTTANKNFPDVSRSQLPLISNGVDGSDYVKDWNSDIQAIKELPTTSIQERILREKLIHKSIFDFNKTATETAINIIKGNLTPMNPTEEEDKHIYLKNGIFYSTGATTVDTFQATGGEEASRYIAAKDLAGVKVMNSRDVRGIYNLVTCIVDYMGKRIVCQAPVPGVLDPAPEDEEESGEKVVYGLSTDANKILEDASFTEPLKQVADVFHLKPHTVELSPEVKSKGELVVSKDSKGLKGTDGRKYVIDLYRTTPRDIEFTEQHFDPSSPQSYPHGEALVRHEAVTEWWKRKIAPLIKAAAEKAEAEGDKTEPVLPTEQVVFNPDAFSTDTESDEDRATVREISKFIKEHLVEEFLNDVSSQLVPFDGKQLTEMLHRAGISMRYLGFIAERIIAKKEEFVAKEAETIKANEEAVKAKKEEEAKKKEEEAKKAEEEAKEEAKEEKKEAEEEKEDEEEPSKATYEPIVANLNVLYTIIVREIVARSSKHILRQLTTGLPTYLVPFAVAHFHNCLLGSAITSTPSVEIDPVYRDFYPESAFEFTKLTTDAVLQLIQTEALVRFRYTLPSDWNSTIRAPQLLREIAHKFGIQWKSQQYAFTKEDFDAQAQDFKVSTIETKSKKKAKKSSPTIVEKTVARSSTFIADDIVNFVPLVKDSSSKSSLIEEIFLSARAHIAAGGKDRETGIALINELVGIQENIYGKVNAETARFYTLVAQMYQQLGYEVEAAILGRKAIVLCERTTGFDSADTITAYMNAAYYEAGNDQTLNSLKIYKHAMQTWASVYGKDHPTLINTLTNSSEAMMRIKAYPAATQFLEEALELSVAVNGNVSEITGIIYFRLANLLVSINKFKESRDLYVIAHDIFQKLLGPDDSLTKQASRYTGSVNMYIEYMNAKSQEAKKQAAAVAAATAKSASKVKVPGVSATTAASKKKAGKKHQVPQPDPEIASKSIDDILKFIEGGNQPKKGKKGSKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG28</accession>
    <name>G3AG28_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_48202</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372579.1">
        <property type="nucleotide sequence ID" value="XM_007372517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG28"/>
    <dbReference type="GeneID" id="18871194"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48202"/>
    <dbReference type="HOGENOM" id="CLU_991013_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG28"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="281" mass="32746" checksum="008021A05C7DFDFF" modified="2011-11-16" version="1">MLYLEHLAEYRRNLSEIILQISGSDLDKQNVQAIFAPFQISEDNFTIETLFMYPQLIPRQYRVSINRLKSLQRVVLYDYRPSQLATALQAQGVENLKIKELQIFTTLAKNDTTGLEYLKDHFDLEYITLLELLVKIPRVTKTLKKKFRRLFSQMVNVKHLFLSRDIVQIENIMDQIQPNTIETFATTFCTFAEVQYALRNQENSVVTIIRDDDSKIGCIKGPFRFVFCRVLAMTKENSAFVKSIQENGKRYPKLKGIIADMCYYTIERNGSDTGLKLKRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG42</accession>
    <name>G3AG42_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_146140</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the APC10 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372592.1">
        <property type="nucleotide sequence ID" value="XM_007372530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG42"/>
    <dbReference type="FunCoup" id="G3AG42">
        <property type="interactions" value="689"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG42"/>
    <dbReference type="GeneID" id="18870707"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_146140"/>
    <dbReference type="eggNOG" id="KOG3437">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025279_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG42"/>
    <dbReference type="OMA" id="HNHLIHA"/>
    <dbReference type="OrthoDB" id="24948at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08366">
        <property type="entry name" value="APC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016901">
        <property type="entry name" value="APC10/Doc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004939">
        <property type="entry name" value="APC_su10/DOC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12936">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12936:SF0">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03256">
        <property type="entry name" value="ANAPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01337">
        <property type="entry name" value="APC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51284">
        <property type="entry name" value="DOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DOC" evidence="7">
        <location>
            <begin position="75"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="332" mass="37330" checksum="57A2C29A25206E70" modified="2011-11-16" version="1">MSSTSRIENTSSISQHEVQLQSHISTDEIRDTIMSSTSENNHHFIDEDEEDEMGNLRINHEEPSEDASTAEQQPNLEQIQRVYYMKGLEELESLQLFDLSPLANWKLSSYKPGFGLVQLRDDSPDTYWQSDGSNQGNNLQHSITIQFSKKVALERISIFTNYSLDESYTPSKIKIMAGSCDGWDMIDVCTVNFNQPIGWSHIIFNGIRNDGVLKCFMVKLIVLANHQEGKDSHIRAIRCFGKKSVRPGANESNNALLHHDLLKDLSMASGSTSLSGLSINTHTMISCDEKENEEEEHEEHVNSSSVLTNVSQVIGFNTGFQSLELKSISSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG44</accession>
    <name>G3AG44_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Glutamate--cysteine ligase</fullName>
            <ecNumber evidence="3 11">6.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Gamma-ECS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 11">Gamma-glutamylcysteine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_58376</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-cysteine + L-glutamate + ATP = gamma-L-glutamyl-L-cysteine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glutamate--cysteine ligase type 3 family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="3 11"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372595.1">
        <property type="nucleotide sequence ID" value="XM_007372533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG44"/>
    <dbReference type="FunCoup" id="G3AG44">
        <property type="interactions" value="403"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG44"/>
    <dbReference type="GeneID" id="18872424"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58376"/>
    <dbReference type="eggNOG" id="KOG3754">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010467_2_0_1"/>
    <dbReference type="InParanoid" id="G3AG44"/>
    <dbReference type="OMA" id="CCPELIG"/>
    <dbReference type="OrthoDB" id="7939818at2759"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00209"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046686">
        <property type="term" value="P:response to cadmium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.50:FF:000009">
        <property type="entry name" value="Glutamate--cysteine ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.710">
        <property type="entry name" value="Glutamate cysteine ligase subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004308">
        <property type="entry name" value="GCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164">
        <property type="entry name" value="GLUTAMATE CYSTEINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164:SF0">
        <property type="entry name" value="GLUTAMATE--CYSTEINE LIGASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03074">
        <property type="entry name" value="GCS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="5 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW35183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="556" mass="63116" checksum="C2562F4B03E06316" modified="2011-11-16" version="1">MYPDKHTKLVDDSIKQRDLFKSDQEPFIGASRPGFVYMDSMGFGMGSSCLQITMQTKNIEEARYLYDSLAPLSPIMLSSTAAAPIFKGHLVNQDVRWNVVSGAVDDRTFIERGVEEYPGYNKFGGLDVDEHTLVKDCSKTNANGDIIGVFANDGKPIQHVPKSRYDSVANYLADSKHDTSYYSDKFNDLNAPINKSVYDRLIQEPNFDHYMANHFAHLFIRDPLVIFSERIDQDNTLENDHFENIQSTNWQTLRFKPPALYESNVDISTTPGWRVEFRPMEIQLTDFENAAYSTFISLLSKAIIKFKPNFYIPLSKVEENMGTAHKVDSAINDKFWFKDLDNWNLDYHEFRGYDLSWFDRYINIGNDELGGEEVYMNGYSVSGSTISLANGELNGAIPNGTVNGNTTNGKRRKSSNGMIITDDAEDLTYAKYSIDQIINGDVSGKFPGLIRLVIKLIATDLIPKSCHCPSTSLANDLTRIKYYLTLISKRAKGEIPTTAHWLRNKVLNHHQYKRDSKVTDFINYDIISDAIKVGDLSDRDVVESLFGEVITDYLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG85</accession>
    <name>G3AG85_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_58432</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372636.1">
        <property type="nucleotide sequence ID" value="XM_007372574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG85"/>
    <dbReference type="GeneID" id="18872447"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58432"/>
    <dbReference type="eggNOG" id="KOG3017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035730_3_2_1"/>
    <dbReference type="InParanoid" id="G3AG85"/>
    <dbReference type="OMA" id="THEVGCA"/>
    <dbReference type="OrthoDB" id="337038at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442486" description="SCP domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="3">
        <location>
            <begin position="110"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="245" mass="27234" checksum="0741D8D97B727282" modified="2011-11-16" version="1">MRYIVPVVFLVNLMVQAAEVYVTETSYVTVSRTKLPDGSITEIILGQPDPIPVDVQLQSQPDQEPVSGPFQQPEPQPEPQPQPEPQPQPQLEPQVQQEPRTQANGVKSSQFADEILDAHNSKRAKHRVAPLQWSQELASYAERYSAAYSCGSGLVHTGGPYGENLASGVSSCSHGVQLWYDEVNGYDYNSQHLNHFTQVVWKSTHEVGCAVKDCGSNGLYLICEYNKPGNIVGDKYFRENVFPPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG98</accession>
    <name>G3AG98_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">YjgF-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_58442</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RutC family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372649.1">
        <property type="nucleotide sequence ID" value="XM_007372587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG98"/>
    <dbReference type="FunCoup" id="G3AG98">
        <property type="interactions" value="548"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG98"/>
    <dbReference type="GeneID" id="18872452"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58442"/>
    <dbReference type="eggNOG" id="KOG2317">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100715_7_2_1"/>
    <dbReference type="InParanoid" id="G3AG98"/>
    <dbReference type="OMA" id="PAKFSHG"/>
    <dbReference type="OrthoDB" id="309640at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00448">
        <property type="entry name" value="YjgF_YER057c_UK114_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.40:FF:000001">
        <property type="entry name" value="L-PSP family endoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006056">
        <property type="entry name" value="RidA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019897">
        <property type="entry name" value="RidA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00004">
        <property type="entry name" value="Rid family detoxifying hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="2-IMINOBUTANOATE/2-IMINOPROPANOATE DEAMINASE RIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803:SF58">
        <property type="entry name" value="PROTEIN HMF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01094">
        <property type="entry name" value="UPF0076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="15394" checksum="E174D152B9AE6097" modified="2011-11-16" version="1">MSAKRSNSTITPVRTSQAPPPAASYSQAIKVNGFIYVSGQIPYTPDNKPLPEGATLAEQAEQVIQNVSNILEASNSSLNHIVKANIFLTDMQAQFGEFNQVYAKYFSEHKPARSCVAVKELPLGVPLEMEVVAVERDDTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGQ2</accession>
    <name>G3AGQ2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Monothiol glutaredoxin-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_58604</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters. Binds one iron-sulfur cluster per dimer. The iron-sulfur cluster is bound between subunits, and is complexed by a bound glutathione and a cysteine residue from each subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Heterodimer with FRA2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family. Monothiol subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372797.1">
        <property type="nucleotide sequence ID" value="XM_007372735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGQ2"/>
    <dbReference type="FunCoup" id="G3AGQ2">
        <property type="interactions" value="879"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGQ2"/>
    <dbReference type="GeneID" id="18872527"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58604"/>
    <dbReference type="eggNOG" id="KOG0911">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026126_12_0_1"/>
    <dbReference type="InParanoid" id="G3AGQ2"/>
    <dbReference type="OMA" id="WAEPCKT"/>
    <dbReference type="OrthoDB" id="415696at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990229">
        <property type="term" value="C:iron-sulfur cluster assembly complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015038">
        <property type="term" value="F:glutathione disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03028">
        <property type="entry name" value="GRX_PICOT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02984">
        <property type="entry name" value="TRX_PICOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000012">
        <property type="entry name" value="Monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000092">
        <property type="entry name" value="Monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033658">
        <property type="entry name" value="GRX_PICOT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="GLUTAREDOXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293:SF73">
        <property type="entry name" value="GLUTAREDOXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="9">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="244" mass="27505" checksum="7A95D98333164F1B" modified="2011-11-16" version="1">MPVIEIKSQEQFTELTQDDSSKLIALYFYTEWAPPCKTMTSVFQTIAESKKDDPSILFLSINADDLSEISEIFEVNAVPYFILIRNATILKELSGADPKEFVSALNQFSNTPEQQAQAPAPAPAPAIQKKEVEEVEESEDELNQRLKKLTTAAPIMLFMKGSPSSPQCGFSRQLVAILREHQIRFGFFDILKDDSVRQGLKKFSDWPTFPQLYINGEFQGGLDIIKESIEEDEKFFEHALEAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGW2</accession>
    <name>G3AGW2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitotic check point protein BFA1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_47726</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372047.1">
        <property type="nucleotide sequence ID" value="XM_007371985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGW2"/>
    <dbReference type="STRING" id="619300.G3AGW2"/>
    <dbReference type="GeneID" id="18871139"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_47726"/>
    <dbReference type="eggNOG" id="ENOG502R6H5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030433_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGW2"/>
    <dbReference type="OMA" id="NYEYTRD"/>
    <dbReference type="OrthoDB" id="19159at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990334">
        <property type="term" value="C:Bfa1-Bub2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034586">
        <property type="entry name" value="Bfa1/Byr4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35140">
        <property type="entry name" value="MITOTIC CHECK POINT PROTEIN BFA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35140:SF1">
        <property type="entry name" value="MITOTIC CHECK POINT PROTEIN BFA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="583" mass="68033" checksum="DFCAD4E46E40F8E0" modified="2011-11-16" version="1">MKSRKVKASTSVPNSYKAQLLTRFADNDNDDEIFGDIEGIDFGHNPSQLNNNQNVLPSGINRHDDGVETLKMEQLVLEDSPINKTTPSPSYHIGDKLDSLKPRSKSSTSSSGEVAHRRVMRECLSEYSEDLDTDVTSEFTDNDFQGLDDIFQGETSMKQRLNAKISEQEKVAEREQEELRMLYSSRREKIIDFDNDPNQTLKLKDFSMFQQDNKLTNENLNLLDMIESENTVNYEYTRDDFAKFEDGFDVNFEQNIQRQIKQKPPIRAKQSMPVLRGNNGSAVKKFKSTMDFGTLRGIREADIDEEEEEEEVAADLPEFHFNNNIIRKLDRIPSFYNNNTSNNNPAKAELLNKYKEQHEHKHKSKIKHQNPKPKLGTVKYLNNNSIITDPVYPTPKKTMKFNAAMNRWEGNEIDLVRFETVTKPTLITFRELQQQKEQPKTKPNMVYDQENLRWVNMNHDDESVFHDIPDLVEAKPYRLNSPPGLTPYPQQPQEPQRISPINRGASQFTQRTMSSLTSKSNLQQDAEQWRQEFQLSGKLIEKFQKEEVKIVKKIKRWFINGIGDYQFNRDYYWEIRKMVMENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGX9</accession>
    <name>G3AGX9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ATP-binding cassette family, regulator of translational elongation</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">GCN20</name>
        <name evidence="6" type="ORF">SPAPADRAFT_132835</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372064.1">
        <property type="nucleotide sequence ID" value="XM_007372002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGX9"/>
    <dbReference type="FunCoup" id="G3AGX9">
        <property type="interactions" value="970"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGX9"/>
    <dbReference type="GeneID" id="18869697"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132835"/>
    <dbReference type="eggNOG" id="KOG0062">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_36_6_1"/>
    <dbReference type="InParanoid" id="G3AGX9"/>
    <dbReference type="OMA" id="CTHIADI"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071232">
        <property type="term" value="P:cellular response to histidine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042327">
        <property type="term" value="P:positive regulation of phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071264">
        <property type="term" value="P:positive regulation of translational initiation in response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006448">
        <property type="term" value="P:regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000104">
        <property type="entry name" value="ATP-binding cassette sub-family F member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000882">
        <property type="entry name" value="Translation initiation regulator (Gcn20)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF117">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="198"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="531"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="282"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="751" mass="84695" checksum="76472C68DEB40455" modified="2011-11-16" version="1">MSNVGLQVRKAVPSIDSVVADYAVGYIQHVSNSTEDIVLAQQVNIDQEMNFIKELLVNAGGSVENVNSLTETIAKQLSSKLSDNMEKLALTGDTSKRLLDINILQNSSKRDINSSLALLSASNDIEHTGRKMESRVDKKKLEKQERKIAKKVAKRNNKFVQYEASKLLNAKKDADYDSFFLEINPLDFGSSAGKSKDIKIDNFDLYVGDGQRILSEASLTLAYGRRYGLVGQNGIGKSTLLRALSRRELNIPKHITILHVEQEIRGDDTPALQSVLDADVWRKSLLQEESKINERIAEIEKLRSEFDEESNEVKKLDNEREDLETHLQEVSEKLTEMESDKAESRAAGILFGLGFTKETQNLPTNQFSGGWRMRLSLARALFCQPDLLLLDEPSNMLDVPSITFLAKYLQGYKSTVLVVSHDRAFLNEVATDIIHQHSERLDYYRGANFDSFYATREERIKNQRREYENQMAYRKHLQEFIDKYRYNAAKAQEAQSRIKKLEKLPLLEPPEDDKVITFKFPEPDKLSPPILQMQDVTFGYSPEKLLIKSVNLDVQMDSRIAFVGGNGTGKTTLLKLLMEQLRPLSGFITRNGRLRIGYFAQHHVDAMDLTTSAVSWMSTAFPGKTDEEYRRHLGSFGITGSLGLQKMELLSGGQKSRVAFAALCLNQPHILILDEPSNHLDTQGLDALADALTNFKGGVLMVSHDVAIIDKVCNEIWISEDNTVTKFPGSIYDYKNHILERADIAGVVRKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH36</accession>
    <name>G3AH36_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ferric-chelate reductase (NADPH)</fullName>
            <ecNumber evidence="3">1.16.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_64584</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2 a Fe(II)-siderophore + NADP(+) + H(+) = 2 a Fe(III)-siderophore + NADPH</text>
            <dbReference type="Rhea" id="RHEA:28795"/>
            <dbReference type="Rhea" id="RHEA-COMP:11342"/>
            <dbReference type="Rhea" id="RHEA-COMP:11344"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.9" evidence="3"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372878.1">
        <property type="nucleotide sequence ID" value="XM_007372816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH36"/>
    <dbReference type="FunCoup" id="G3AH36">
        <property type="interactions" value="394"/>
    </dbReference>
    <dbReference type="GeneID" id="18874983"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64584"/>
    <dbReference type="eggNOG" id="KOG0039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010365_4_0_1"/>
    <dbReference type="InParanoid" id="G3AH36"/>
    <dbReference type="OMA" id="MRINAHI"/>
    <dbReference type="OrthoDB" id="167398at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF9">
        <property type="entry name" value="FERRIC REDUCTASE TRANSMEMBRANE COMPONENT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="7 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442069" description="ferric-chelate reductase (NADPH)" evidence="17">
        <location>
            <begin position="20"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="346"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="18">
        <location>
            <begin position="408"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW35466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="714" mass="82752" checksum="799D468190951FA6" modified="2011-11-16" version="1">MNLGYSLYLLFILVVGVLGEKHDFEWYNKSIPFHACAYEIKNAENFKFCPTMDGVCICSNENALATLAGCLAYKDENTTYFAHYGISYCHVRFKINLESDWFDTAFKRFTNKAVNQSEIESRYKLIQTPVIFSSKDMDFYDESAAVFFDNYNISTFFGLSVFGFWLVFLLINTISYWTKVLFPRATRRSVGLLATFYRKHISMPALYGKRRTQEVSCFKVFDSLVPSRFEMMVISLFYIFILIIHAVRVHGLKDDPIFGSKYMAELRYVGDRTGIVATFMMPLLFLFAGRNNFLQWVTGINYSSFLCYHRHIARIMFMLVVIHSVNYTILLVRTDQFIVTLKKQCLRWGITATIVGGTLIIQSILYLRRNWYELFLLIHIVMAIFYVVGTWVHLNQFGYEIYLYSTIGVWGLDRIIRLFRLLSFGFPKVNVMLVGDDVIKLEIPKPKNWKPIPGGHAFVHFLKPTYFWQSHPFTFTNSTKDNKDRVTMYLRVKGGVTHSLYQLLNTTPGKMVKMRVGIEGPYGESTPAKYADKAIFFAGGNGIPGIYSEVSDIALRSSPQRKSILKLYWIIRDYDSILWFFDELLKLKDMNIETTIYVTRPNIKSFRVDLFNQSQSSQCSSIKSGYEETKSNTGDIELAIAELKLKLLHIKFEEGRPNVEQLIENEIKQSPGSTAFVACGHPIMVDEVRYYSCKNIDNSYNKRVDYYDQLQIWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH88</accession>
    <name>G3AH88_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA/rRNA methyltransferase SpoU type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_58751</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372930.1">
        <property type="nucleotide sequence ID" value="XM_007372868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH88"/>
    <dbReference type="STRING" id="619300.G3AH88"/>
    <dbReference type="GeneID" id="18872589"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58751"/>
    <dbReference type="eggNOG" id="KOG0839">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_450684_0_0_1"/>
    <dbReference type="InParanoid" id="G3AH88"/>
    <dbReference type="OMA" id="IEWIIAY"/>
    <dbReference type="OrthoDB" id="241340at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016423">
        <property type="term" value="F:tRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18091">
        <property type="entry name" value="SpoU-like_TRM3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000022">
        <property type="entry name" value="Trm3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045330">
        <property type="entry name" value="TRM3/TARBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044748">
        <property type="entry name" value="Trm3/TARBP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029:SF11">
        <property type="entry name" value="METHYLTRANSFERASE TARBP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029">
        <property type="entry name" value="RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="3">
        <location>
            <begin position="456"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="606" mass="68914" checksum="9747C74F1ADBC63D" modified="2011-11-16" version="1">MNSNSTDYKSNLSMVQTIKGYLDSGVGRIDQIVELLTSLWFNLEAGRLQLNQKNLHVLLIQTLFHKNVLDYSKENEEVSELLVKFSESVIANGITRRSLLPELTKCILNYEQELDWLVPVLIDAFLVYQLRNNSFRLESIISRIYDEQISLTKSDIYADTYGDEEISARVNLMAIFNSIKKLFNIVNNVDSIETWNRIQLWTIVLSIVDKVSIDYKLILNMIETDKSPMIRVYLEWILSYKLLTDKNEQIVNQMFDTLASNTITPTIVISYEKILYLMIKQAPTVEQVTKLLNIIIPGSTSNKALTRHFSLSLIMSIHSNDKIMSILSQDMLHVVDSIYSNTMKSDSINDHKTGDALLWDITEFTLCNLSGGLIYRLYNRDDVDYILPSQYQRYLSEEHVKKLNHPIGDVIQEKQAQVGKKVSKALTGNPLQTKSGAWTTIMDIDEGRQDIVRSDLIVVSSLVDKPPNLGGICRLCDVLGAGTLTLNDMTVTQHPQFKNVAVTADHWMPMIEVKMEEIKDYLIAKKKEGYTLIGLEQTTESVELNNKLEFPKKSLILLGKEREGIPGDLLSELDFCVEIKQVGVIRSMNIQTATAIIVHAYSSQHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHM9</accession>
    <name>G3AHM9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein mL53</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_135544</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL53 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373778.1">
        <property type="nucleotide sequence ID" value="XM_007373716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHM9"/>
    <dbReference type="FunCoup" id="G3AHM9">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHM9"/>
    <dbReference type="GeneID" id="18869899"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135544"/>
    <dbReference type="eggNOG" id="ENOG502S452">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131037_1_1_1"/>
    <dbReference type="InParanoid" id="G3AHM9"/>
    <dbReference type="OMA" id="MDFNCSK"/>
    <dbReference type="OrthoDB" id="46988at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019716">
        <property type="entry name" value="Ribosomal_mL53"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042776">
        <property type="entry name" value="Ribosomal_mL53_fung"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28236">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L44, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28236:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10780">
        <property type="entry name" value="MRP_L53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="92" mass="10427" checksum="4C9A68649B624EA5" modified="2011-11-16" version="1">MITKYFANVVVKFDAFGVGARNARIFLSQVPTTAKIDCKVLAPNSQDEQQIKVTFKDKHVMSIDPCNTSINQMKEYFDAHSRKMAIEASIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHP0</accession>
    <name>G3AHP0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinase</fullName>
            <ecNumber evidence="4">2.7.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_134383</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <dbReference type="EC" id="2.7.-.-" evidence="4"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373788.1">
        <property type="nucleotide sequence ID" value="XM_007373726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHP0"/>
    <dbReference type="FunCoup" id="G3AHP0">
        <property type="interactions" value="204"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHP0"/>
    <dbReference type="GeneID" id="18869808"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134383"/>
    <dbReference type="eggNOG" id="KOG1620">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042569_4_0_1"/>
    <dbReference type="InParanoid" id="G3AHP0"/>
    <dbReference type="OMA" id="ACMTAKC"/>
    <dbReference type="OrthoDB" id="2573163at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000824">
        <property type="term" value="F:inositol-1,4,5,6-tetrakisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008440">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF21">
        <property type="entry name" value="KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="329" mass="38453" checksum="9C26A3D67095F1D2" modified="2011-11-16" version="1" fragment="single">ELRPFKNKVGGHTAIFSFSKRAVCKALVNRENLFYETIELKRPQLLKFMPKYIGVLNHTRFERFILLEDLTTNIKQPCVLDLKMGTRQYGIEATCKKQQSQRRKCLATTSRKLGVRVCGLQIYKYNNQRLVHDKYYGRKITIGKQFCKILAKFLYNGHDNYSVMFRIPDLIDQIKELYSIFQELPGYRMYGSSILLMYEGGGERYDHVNVKIIDFAQSIISEEGSHQNAKIPPQHPNLVDLGYLRGLTSLIAYFRVIFRVISGKNFTNTEDMKIWVKENGNRLKHENNHWLDEFAEHINGEINDEELPDDPFDIDYPEYTDVDDEGISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI08</accession>
    <name>G3AI08_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">MICOS complex subunit MIC60</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Mitofilin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_59737</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays a role in keeping cristae membranes connected to the inner boundary membrane. Also promotes protein import via the mitochondrial intermembrane space assembly (MIA) pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the MICOS complex subunit Mic60 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373906.1">
        <property type="nucleotide sequence ID" value="XM_007373844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI08"/>
    <dbReference type="FunCoup" id="G3AI08">
        <property type="interactions" value="202"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI08"/>
    <dbReference type="GeneID" id="18873057"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59737"/>
    <dbReference type="eggNOG" id="KOG1854">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008024_2_0_1"/>
    <dbReference type="InParanoid" id="G3AI08"/>
    <dbReference type="OMA" id="RLDHQMQ"/>
    <dbReference type="OrthoDB" id="10261039at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019133">
        <property type="entry name" value="MIC60"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15415:SF7">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15415">
        <property type="entry name" value="MITOFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09731">
        <property type="entry name" value="Mitofilin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="283"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="562" mass="62530" checksum="41DE779C2CDAE593" modified="2011-11-16" version="1">MIRSVVSRSTRTASRRGTRWMSQSAIRSNVAKVEAPPLPPAVKPAAKKQKKFSLIGALIKLTLLAGTVYGGTLYVATKNDKVMDFVVEQQLPYHEELLEYIDNFDIDDIKKSYDTLINKINGVKLPTKEDVESITHELEHRGEELVKETKKIFTQAVDIPKSVPEPVEKDTALTPAQQLQKVEIEPVKREIGHLPLIELNSDIAKSVDSTVKSTIASFNKFIQNIDANTLASSDAAVVKTISENINQLATKLNSLTKTFDDELQSKLKVSQTELFSSFTKKELELTENLLEQFNKEKSQLEAKLNEKLANEIQASREAIAQAASNAVSMVRIEQTKNFEKLVAEKLDQERNGRLANLESLHSRVEELEKFAESFEHQLVNNHSRSLIQQSISKIRGLLLAPTADDKPQHLAPHVEALASVANDDEVLQLALKDLTPLLSGESTHSILTPSQLLSRWEQLAPELRSASLLPPNAGLLGHLSSLLFSKLLLPVKGVKAEGKDIESVIARVESSLTRGELDVAVEEASNLKGWCRKLADDWVTEGRKRLEVEFLLNLIESEVKIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI21</accession>
    <name>G3AI21_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lipid droplet-associated hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_54487</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. LDAH family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373919.1">
        <property type="nucleotide sequence ID" value="XM_007373857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI21"/>
    <dbReference type="FunCoup" id="G3AI21">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="GeneID" id="18871868"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54487"/>
    <dbReference type="eggNOG" id="KOG3975">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018394_1_1_1"/>
    <dbReference type="InParanoid" id="G3AI21"/>
    <dbReference type="OMA" id="LIGYYHT"/>
    <dbReference type="OrthoDB" id="448051at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019363">
        <property type="entry name" value="LDAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13390">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13390:SF0">
        <property type="entry name" value="LIPID DROPLET-ASSOCIATED HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10230">
        <property type="entry name" value="LIDHydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="37392" checksum="CF6CF84CF29F8A9B" modified="2011-11-16" version="1">MLNLLDIEPKTTIYHKLPLKNLDGDECDLLLMIPGNPGLIEFYMTYLDLIQEQFPYMEILGISHAGFSGSAISEKTYDLSFQISHKYDIVKKFILDKYASAGKKTNLYTLAHSMGCYVYQRALWEILHDKELDGKVTVKFSGLITPTILDIAQSPSGTKVNFMINWNIPFIWFALLLCSFLNLILSEEAMQNVVALRLIPRRKRKALGVENSIVGGVKLLKSKEIINQALTLASEEMKEINSDHKINDWYFHHLSDKKWVFFANKDRWIGNKTREYLIQQYGNGSGQKFEICNNKSEPIMHNFCVSQSEEFADITVNRIKALCESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI60</accession>
    <name>G3AI60_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)</fullName>
            <ecNumber evidence="4">5.4.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">PGM2</name>
        <name evidence="17" type="ORF">SPAPADRAFT_59795</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-seryl-[protein] + alpha-D-glucose 1-phosphate = alpha-D-glucose 1,6-bisphosphate + L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:68748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:58392"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>alpha-D-glucose 1,6-bisphosphate + L-seryl-[protein] = O-phospho-L-seryl-[protein] + alpha-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:68752"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58392"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23536"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="5.4.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.2" evidence="4"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373958.1">
        <property type="nucleotide sequence ID" value="XM_007373896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI60"/>
    <dbReference type="FunCoup" id="G3AI60">
        <property type="interactions" value="645"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI60"/>
    <dbReference type="GeneID" id="18873079"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59795"/>
    <dbReference type="eggNOG" id="KOG0625">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009330_0_1_1"/>
    <dbReference type="InParanoid" id="G3AI60"/>
    <dbReference type="OMA" id="WIQDRAN"/>
    <dbReference type="OrthoDB" id="2291at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004614">
        <property type="term" value="F:phosphoglucomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019388">
        <property type="term" value="P:galactose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019255">
        <property type="term" value="P:glucose 1-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-alpha-D-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.50:FF:000002">
        <property type="entry name" value="Phosphoglucomutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000004">
        <property type="entry name" value="Phosphoglucomutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000005">
        <property type="entry name" value="Phosphoglucomutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000006">
        <property type="entry name" value="Phosphoglucomutase PgmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045244">
        <property type="entry name" value="PGM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005737">
        <property type="entry name" value="PRK07564.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22573:SF2">
        <property type="entry name" value="PHOSPHOGLUCOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22573">
        <property type="entry name" value="PHOSPHOHEXOMUTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24947">
        <property type="entry name" value="PGM1_C_vert_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="14">
        <location>
            <begin position="17"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="15">
        <location>
            <begin position="188"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="16">
        <location>
            <begin position="306"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW34374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="560" mass="61150" checksum="18F9E3F487BC068B" modified="2011-11-16" version="1">MSDFTVQTIETTPFQDQKPGTSGLRKKVTVFKQPHYTENFIQAILDAIPEGAQDATLVVGGDGRYYNDVVIDLIIKIAAANGVKKLILGQGGVLSTPATSHVIRIRKATGGIILTASHNPGGPTNDLGIKYNLGNGGPAPESVTNKIFEISKKLTSYKLIDLPKVDITKIGESKVGPIDVEVIDSTKDYVDMLKSIFDFPLIKSFLDKATKEENFKVLFDALNGVTGPYGYEIFVNELGLPLESIQNYKPLPDFGGLHPDPNLTYAHTLVSRVDKEGIAFGAASDGDGDRNMIYGAGTFVSPGDSVAIIAEYADVIPYFKKQGVYGLARSMPTSGAIDLVAKDKGLNVYEVPTGWKFFCSLFDSDKLSICGEESFGTGSNHIREKDGLWAIVAWLNVLAGYAAKNPGSKTSIEIVQNSFWEKYGRTFFTRYDYENVSSEGANDLVKLLEGIVNSSKPGDELKPGFVVKEAANFSYTDLDGSVSSNQGLFIKFENGLRFIVRLSGTGSSGATVRLYLEKHSNDKSTYQLKVDQYLSKEVEFVLDLLKFKEFLGKEEPDVRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI66</accession>
    <name>G3AI66_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">DNA-directed RNA polymerase III subunit RPC3</fullName>
            <shortName evidence="9">RNA polymerase III subunit C3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">RPC3</name>
        <name evidence="13" type="ORF">SPAPADRAFT_70500</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373963.1">
        <property type="nucleotide sequence ID" value="XM_007373901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI66"/>
    <dbReference type="FunCoup" id="G3AI66">
        <property type="interactions" value="505"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI66"/>
    <dbReference type="GeneID" id="18875329"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70500"/>
    <dbReference type="eggNOG" id="KOG2587">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010734_0_0_1"/>
    <dbReference type="InParanoid" id="G3AI66"/>
    <dbReference type="OMA" id="KHRFVRH"/>
    <dbReference type="OrthoDB" id="272392at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055207">
        <property type="entry name" value="POLR3C_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013197">
        <property type="entry name" value="RNA_pol_III_RPC82-rel_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008806">
        <property type="entry name" value="RNA_pol_III_Rpc82_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039748">
        <property type="entry name" value="RPC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949">
        <property type="entry name" value="RNA POLYMERASE III DNA DIRECTED -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08221">
        <property type="entry name" value="HTH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05645">
        <property type="entry name" value="RNA_pol_Rpc82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20912">
        <property type="entry name" value="RPC3_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22536">
        <property type="entry name" value="WHD_POLR3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase III subunit RPC82-related helix-turn-helix" evidence="11">
        <location>
            <begin position="17"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase III Rpc82 C -terminal" evidence="10">
        <location>
            <begin position="149"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase III subunit RPC3 winged-helix" evidence="12">
        <location>
            <begin position="442"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="602" mass="69036" checksum="10C99F4DDFD9CC81" modified="2011-11-16" version="1">MDETITEAAKTQSTYSFLYTSIAQTHLGEVASCIISCLISNGRLTSKELSQRTKIPLKSIKSALVSLVQLNCVYYWTSKDSAKTVHYSLNESGLLAFIHSGDIIQHIKERYGDDAAEIIQNVLINGHVKVEDYLGQYEDLEVRMEKQTLFFKLFSERWLVRLQPFNFSPIDDIWQEVFQECLTNTPRTTTTSEIKRVAEAKEKAKAKLTTLLESGQSPQDLYDSKDGIKKLQPHIIVTFNVSRFQKHLRTNSLVNFAKSRIGLLTAKVYEAALKVVEQNSPELRHPFLEISGLINDPEEEKFFINSIENKLVDERKIVFNIRDVMRTLSDDIDLRNSILTHNFLKPQLKKRPLEDSEEEATKKVKLENDQSVTIKNEGESSDVGNGNAFTDNSDPHSLTLIQHHLKLLTSGTAIQFLNEISPGTFSVPYAYLTKQIKQYNFESLIKTTLGQDAFRILRCLKSMKLSDEKTISNAVLLKEKTVRNEIYQLVKANFVEIQEVPRSADRAASKTFFLFRFKEWPSNKFLLNSLIYSMAEIINNVQDFKADHKILLEKCEREDVQGHEEELLLESELKTLLGLQNREINNIGKFNRIKTLYDIFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI99</accession>
    <name>G3AI99_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MATE efflux family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_59037</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373252.1">
        <property type="nucleotide sequence ID" value="XM_007373190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI99"/>
    <dbReference type="STRING" id="619300.G3AI99"/>
    <dbReference type="GeneID" id="18872735"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59037"/>
    <dbReference type="eggNOG" id="KOG1347">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_1_2_1"/>
    <dbReference type="InParanoid" id="G3AI99"/>
    <dbReference type="OMA" id="VKFCLYQ"/>
    <dbReference type="OrthoDB" id="2126698at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="383"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="416"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="441"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="482" mass="52607" checksum="5E191DE7E39871A6" modified="2011-11-16" version="1">MNLNTEETLLTQNAEYQHYDAILPDEEAEKVTITSVSELKRISISSIPLVLTFTLQYFLSITSMYAAGRLGAKELAACSLALCTFNITGLAVYQGMASSLDTFCSQAFGSGNIKMVGVYFQRCSVIILTISVPLVILWFNSGAILKTMVPDAELAEMAETFLKIHAIGAPGLILFETGKRFLTAQNIFNAGSYILVMIIPINLILNWLLVWHPTYGLGYNGIALAIVITYWLIDFFMLAYVVFIDGKQCWGGFDLRKAFTNWKPMLSLAIPGVIMVIAEFLAFEVLTILAARFGTESLAAQSIASNVASLAFQLPFAIAVALSTNIGNYVGQKNVAAAKCNSNLFAILASCTGTINFAIMFFGRHTIGKLFTNDEKVLTITTTLLILTAINQFGDSLVVIGSGVLRGQGRQRIGSILNMISYYLVALPVGFYLAFNCHLDLNGLWLGLITGVAFLSISTFIAVYLSNWPQIIEKSHGMHDSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIC5</accession>
    <name>G3AIC5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein KRE1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_48818</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373278.1">
        <property type="nucleotide sequence ID" value="XM_007373216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIC5"/>
    <dbReference type="STRING" id="619300.G3AIC5"/>
    <dbReference type="GeneID" id="18871271"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48818"/>
    <dbReference type="eggNOG" id="ENOG502SASR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156717_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIC5"/>
    <dbReference type="OMA" id="QSFKTTY"/>
    <dbReference type="OrthoDB" id="5406216at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031452">
        <property type="entry name" value="Kre1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17056">
        <property type="entry name" value="KRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442357" description="Protein KRE1" evidence="1">
        <location>
            <begin position="19"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="12237" checksum="D340D6947B3D44B9" modified="2011-11-16" version="1">MKFYILLLSLLTLVYSLGKQDDGQDQQTTTTTQQTIVWVTTTINGQLATLSTLYSQKFTEKATGTTEVQSGQIGLGTISGSVGGIREYTSVTITPTAGAAGTSGAGLLGLFAVVVGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIE2</accession>
    <name>G3AIE2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VPS9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_49455</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373996.1">
        <property type="nucleotide sequence ID" value="XM_007373934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIE2"/>
    <dbReference type="STRING" id="619300.G3AIE2"/>
    <dbReference type="GeneID" id="18871340"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49455"/>
    <dbReference type="eggNOG" id="ENOG502T6N3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019463_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIE2"/>
    <dbReference type="OMA" id="QILDKWF"/>
    <dbReference type="OrthoDB" id="10264848at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053097">
        <property type="entry name" value="Prespore_vesicle_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34586:SF3">
        <property type="entry name" value="FOLLISTATIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34586">
        <property type="entry name" value="SPERACT/SCAVENGER RECEPTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VPS9" evidence="2">
        <location>
            <begin position="163"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="451"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="524"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="591"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="650"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="714"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="796"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="467"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="534"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="599"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="650"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="714"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="856" mass="97675" checksum="FC0F4A958FEDE3FD" modified="2011-11-16" version="1">MSSPYKLLSVLHEEVKANLTLHPQISSLIQLFLKYLKEPRYQSPLTVAELSHLFHKFYQDLNVLTIGIFTQSNSTKKQLISNCQFFNSYPKNFDYLLAIANYSTSSIKLLKRSDDDALYQLRVFNYYKFLIIFESLNLAQLELFNSINIGDEVTLYDKIFKFDEKDTVYQEFFDDKLSNLKKLNLSFKLFIDHDVGDQDHLIEVMDNIDKEDLSVLQENLIDLEKTERNPFSKLISIVKLHKNLIKLFINKGFENKNINNDLLLPSLIYLLIYKLDNEELYLNFLFIKNFLNVLDTNPVELYNFNLNSSYNPTQERLASASGTAKVKRGNIFELLNLFEIQDQAQSQEFVIDDDFRFFNNDKDLITYLSHGFLNTGELYFYLTNFEAIIEYLANCTVSELLSKEEEHLNIDPDLRRLNVLNIPISKLVEEELLTHFQFPDGALQEEIKLKQEEEQSQQSQRDKSRSRSSSLINTITNRINDTRTRSNSSLKQEIFPSLASAEESIEGTGLSMMRNIIGRFSSASSSSVPQISDNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNQEQQQPIISPDKRSNSLLNRVAPTHSRTRSSSLEASISNPFTHSKRNSITSKFATGVTEFMTKLNQQQPQQLSSVPNLQNTVSESNNISNSSLQSLDNGYSNGSNTVDAASCSSTVVPKPEAGRSRTTSLQILDKWFNNISANAPQQSQNTISQYSPISPAHPRVKSYPLPTNHEEPVNAIDDVEQLTKYHNVDFESLSIKQLRELKQFYDHFCFGLLSNNYDKGSGVVTDKSPSRNNSLDNDKSPSRNNSLDKLMAKFDTTDLNSKAESIDSNEKITNGNISTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIJ6</accession>
    <name>G3AIJ6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59079</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373295.1">
        <property type="nucleotide sequence ID" value="XM_007373233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIJ6"/>
    <dbReference type="STRING" id="619300.G3AIJ6"/>
    <dbReference type="GeneID" id="18872761"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59079"/>
    <dbReference type="eggNOG" id="KOG1269">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057148_2_0_1"/>
    <dbReference type="InParanoid" id="G3AIJ6"/>
    <dbReference type="OMA" id="MIHVWAR"/>
    <dbReference type="OrthoDB" id="10017101at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="37"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="279" mass="31285" checksum="4AD93061FD386ADC" modified="2011-11-16" version="1">MVKDQAYYANGYNKSISAIHALRTVESNVSYVLDVLKPNFKVLDVGSGPGTITVDFGANYLTEGGSIIGVEPTPEILEDAEAYKKKYSEEHGKNLDNVSFQKGSIYKLPFEDNSFDLVHAHQVFLHLQDPVAGLKELKRVAKQGGFVCVKDGDIESTLIYPEEYEKLRDIYVLKAKNSGYTDPRAGRKLRGRAIEAGYEPANIKTTVSNRVFGDDQDTKKLMGKLLIDRAKNGGEEFYPDDLERSKRVREESIELLTKFTNDITSLDVSSSFQIIYHKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIT9</accession>
    <name>G3AIT9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_65637</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374089.1">
        <property type="nucleotide sequence ID" value="XM_007374027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIT9"/>
    <dbReference type="FunCoup" id="G3AIT9">
        <property type="interactions" value="855"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIT9"/>
    <dbReference type="GeneID" id="18875052"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65637"/>
    <dbReference type="eggNOG" id="KOG1969">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004894_3_1_1"/>
    <dbReference type="InParanoid" id="G3AIT9"/>
    <dbReference type="OMA" id="RWLKGWE"/>
    <dbReference type="OrthoDB" id="2195431at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF3">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY PROTEIN 18 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="199"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="713"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="713"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="793" mass="89414" checksum="569FEDD638E62FE5" modified="2011-11-16" version="1">MATVDEFTMEVDSINLSESTLFTQERNDNDYDFQMTDSLLFAEPEKVQEQPGVIEEKEEDDEEEDDILTSHISQPFKEVKLFNGRTVKLKPKTKSATVQAPVQGSYMDMDTLFSKANLHNTIKENNRKLAEMEEHTPVASTSSQIWTEKYRPTKFLQLCSAGNDKQYRLVLHWLKKWSSVVFGEQVHDSDHSDGLGRPHRKILLIHGPPGIGKTAVVHILAKQMGYAIQELNAANSMDILPQSAANGSNPYANAASALKLKILNALTSNSLTSTTSKPSCLVIDEIDSLANANDVVKVLNDLVASDQKALNKKLKKASTEETSKKSKKKDVLLSRPIICIANEIYSNQNGKFGPNPMDKLRPIAEIVPFRKPQSGKTTTGQKFGGNAMKSVKDHLMWINEREKLGLDSRAIGDIVDTCDGDIRACINYIQFNAKKTGTANDSSMDKQISWYSMVDELFRREPQLKKDENFMFLLDKFMNGTGKSVVSNSSSFDRVIMGSFNRYLDTVHLQDDSLIRPSEFSDWLGFYDQISSSNKDNQYSGLVGLKIWSLFSQMNPQKNGAKLIPNARNLDFDSFEALKQNKATVKRITDKLPLQTQLSLGVNGDSMAGYFLPYMSKLLAPSLSSKIKSTLSETEQGWVRKIASIVKDFNLSLLNVKDIETGVVSLQISPNWDSLTLYENEFTLLPSATIVKQTQIRRQNLFPLISAELERHEISRKSQKRPNETEPEESAKKRFRGGNSVDFFKGKYQDMNTQIKSETPDDDFQPARIWVKYHEGFSNAVRKNIGWKDIWLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIX5</accession>
    <name>G3AIX5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATP-dependent rRNA helicase RRP3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP-dependent rRNA helicase rrp3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_59141</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DDX47/RRP3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373370.1">
        <property type="nucleotide sequence ID" value="XM_007373308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIX5"/>
    <dbReference type="FunCoup" id="G3AIX5">
        <property type="interactions" value="1165"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIX5"/>
    <dbReference type="GeneID" id="18872792"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59141"/>
    <dbReference type="eggNOG" id="KOG0330">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_1_1"/>
    <dbReference type="InParanoid" id="G3AIX5"/>
    <dbReference type="OMA" id="GIGIKCC"/>
    <dbReference type="OrthoDB" id="10261904at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17954">
        <property type="entry name" value="DEADc_DDX47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044765">
        <property type="entry name" value="DDX47/Rrp3_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF24">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14 19" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="18">
        <location>
            <begin position="63"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="94"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="292"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="447"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="13">
        <location>
            <begin position="63"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="447"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="466"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW33786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="475" mass="53015" checksum="791E689C63002410" modified="2011-11-16" version="1">MKDSKITKKKSSKIDATKKLAEKIKQQALKNQQSTTTTTTTASTSSTTITPIEVNPDEELKFKSFSELKLVPELLESIQSMKFTKPTPIQSEAIPHALEGHDIIGLAQTGSGKTAAFAIPILQALWEAQSPYFALVLAPTRELAFQIKETFDALGSTMGLRSVCIVGGMDMMDQARDLMRKPHVIVATPGRIMDHLETTKGFKLKDLKYLVMDEADRLLDMDFGPALDRILKVIPTKRTTYLFSATMTNKIEKLQRASLHNPVRVAVSSKYQTADNLVQSMMLVHDGYKNTILIHLLSEYEGKLIIVFTRTVAHAQRTALLCRILGFNAVPLHGQLSQAQRLGSLNKFKSRSANILIATDVAARGLDIPSVDVVINYDIPTDSKAYIHRVGRTARAGRSGKSISLITQYDLEMYLRIESVLGKKLPKEDMPPKEVLDALHPNVDRATAEAIRQTKELHEKRGKRRNRDDADREER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ10</accession>
    <name>G3AJ10_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_59955</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetate uptake transporter (AceTr) (TC 2.A.96) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007374044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ10"/>
    <dbReference type="GeneID" id="18873151"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59955"/>
    <dbReference type="eggNOG" id="ENOG502QUJS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051062_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ10"/>
    <dbReference type="OrthoDB" id="3648309at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015123">
        <property type="term" value="F:acetate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051633">
        <property type="entry name" value="AceTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000791">
        <property type="entry name" value="Gpr1/Fun34/SatP-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038013">
        <property type="entry name" value="AceTr_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123:SF1">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01184">
        <property type="entry name" value="Gpr1_Fun34_YaaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="262" mass="28024" checksum="8BD08FD5981595EB" modified="2011-11-16" version="1">MSKNLEVDSIRDLPSSFDESTSSINKTITKVVTDNEFVILGNQKFYTHELMSVFGPTLNLGITPVSANKIGNPAPVGMLTFGLTCFIMSLYGVHAGGITVSNVVVGMLIFVGGTGQALCGVGEMIIGNTFGATVMLSFASFWFSYGAVLIPSFGIEEAYAEDMDQFNNALGFLLMGYAILASWLTTFTFKASISILILFVCITMTFLLQSIGAMTGNSHVAYAGQIFGIFTGLSGMYNGLVDLALKETTYFTLPVGPLNSHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ39</accession>
    <name>G3AJ39_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dethiobiotin synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_70642</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374135.1">
        <property type="nucleotide sequence ID" value="XM_007374073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ39"/>
    <dbReference type="FunCoup" id="G3AJ39">
        <property type="interactions" value="109"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ39"/>
    <dbReference type="GeneID" id="18875339"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70642"/>
    <dbReference type="eggNOG" id="ENOG502RXZU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072551_2_0_1"/>
    <dbReference type="InParanoid" id="G3AJ39"/>
    <dbReference type="OMA" id="NPIVIFQ"/>
    <dbReference type="OrthoDB" id="425114at2759"/>
    <dbReference type="UniPathway" id="UPA00078"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004141">
        <property type="term" value="F:dethiobiotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03109">
        <property type="entry name" value="DTBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00336">
        <property type="entry name" value="BioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004472">
        <property type="entry name" value="DTB_synth_BioD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00347">
        <property type="entry name" value="bioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43210">
        <property type="entry name" value="DETHIOBIOTIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43210:SF5">
        <property type="entry name" value="DETHIOBIOTIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13500">
        <property type="entry name" value="AAA_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006755">
        <property type="entry name" value="DTB_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="23572" checksum="7D89AE8745924521" modified="2011-11-16" version="1">MRVVFITGTDTDVGKTFISALFTKAWNANYWKPIQTGLESDQGDTETVKELTSMPKGQFERPQVELKFPLSPWRAAIKENKPQTKVNEIKIPAKFLNSPRPLIIEGAGGLYVPINETEITTDLILHFDVPVILVARSGLGTINHTLLSLEHLRNHGIHKVYVVMNGPINADNAEAIEGFSEGIKVIASIPYSKSNEIESLLSYVPSLDSIEMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJE6</accession>
    <name>G3AJE6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NDT80 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_135206</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374189.1">
        <property type="nucleotide sequence ID" value="XM_007374127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJE6"/>
    <dbReference type="STRING" id="619300.G3AJE6"/>
    <dbReference type="GeneID" id="18869873"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135206"/>
    <dbReference type="eggNOG" id="ENOG502SAHY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_397387_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJE6"/>
    <dbReference type="OMA" id="TRVIKYF"/>
    <dbReference type="OrthoDB" id="4117572at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1390">
        <property type="entry name" value="NDT80 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052605">
        <property type="entry name" value="Fungal_trans_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024061">
        <property type="entry name" value="NDT80_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037141">
        <property type="entry name" value="NDT80_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35144">
        <property type="entry name" value="MEIOSIS-SPECIFIC TRANSCRIPTION FACTOR NDT80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35144:SF1">
        <property type="entry name" value="PROTEIN PACG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05224">
        <property type="entry name" value="NDT80_PhoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51517">
        <property type="entry name" value="NDT80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NDT80" evidence="4">
        <location>
            <begin position="322"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="NDT80" evidence="2">
        <location>
            <begin position="322"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="61"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="188"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="251"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="670"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="702"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="75"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="188"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="312"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="670"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="706"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="812" mass="91416" checksum="91BA82E6470D3BD6" modified="2011-11-16" version="1">MNDATTKDQLEDLASLFLPDILHPIHSAGNTPGGTNLKTSASTGHTTTSANLIKQEEYDGLSPDFNLLPRDSIGSSSNTPSATTTTNTTATNTTNFQDFLSNNNFINRSNNKSNMMSSYMMPNDSQSDQLYSQQSQQQQQQQPSYSHVNHYAPYIPYEANAGMASASSHHGHHGHQQQHMTMSLINSTFQPYPSQQGSNQMSPSPMPTQQQQQQYGSYQYPNTLNSSLSSTYLNGLSGWYPSNNSSTTNFDNLMTTGGYQQQQQPQSYVPDILQQQQQQSAQSQQNSQPQQSQSMEGIQIQYDERESSSSLPKKKKVKGRRNRNTKATDVEIDYKPFKLKRLLDFKQSGVTSSNDYKLIDKDNNEIEIDFCGFLNGRFLTNDIDNNNYIFTKNELQRESGEETVPKVVNKKEDPKVISCYRRNYIQISLNVNIHGFKGSNSKLLKLQTSEYGYTITRVIKYFKIEILAATNISNSKNVPILIRNDPKDIEKDKDKDLKKNVQANYEYKDDLVKPGPITSQEHILILNDECQIENGAIDKFFVIKKLQFKNATPNNGNLTFQNYYHLKIKLSCIVADLYYDDYIEEDGAIGNDASAATTNNGGDNNEIILAELISEPIIVRGRNPSFYAERKDILIKGRHPNSRGSFKMATQPISHINVQVDDQDLDYHDEDDEEGVAAGEDEEASGSGPANMEEDDDDQHIQTINPEEDNNEDDGSPISFNNEEQRARAGSLVTTNSQIPPLAYSTNQSALDVKNVKKYKYFPISNVYYLPPINVVYFPHRAHQSQKGEEADPQVEPAVVNHTKKSSNVYFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJZ3</accession>
    <name>G3AJZ3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59467</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373628.1">
        <property type="nucleotide sequence ID" value="XM_007373566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJZ3"/>
    <dbReference type="STRING" id="619300.G3AJZ3"/>
    <dbReference type="GeneID" id="18872929"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59467"/>
    <dbReference type="eggNOG" id="ENOG502RIY1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1163571_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJZ3"/>
    <dbReference type="OMA" id="MWADAED"/>
    <dbReference type="OrthoDB" id="4026150at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="239" mass="27346" checksum="3AEEF89052F4C62C" modified="2011-11-16" version="1" fragment="single">MSKLVSRWADDDSLVEEAIKQDSKVNTKHTRESDWHKPITVKANEALPSRWADADDEPAPVPEPIEKSPKKYNRYEQHKQRGDFEHRGGRQGGRGDYPTPPHSRHSDEMKQGSYPSKADRYSNRRPVKREPEPEQDDEQPEEDLDDVELEKGPMTDAAKAFAARLGISAGGFDKGSTKKNSKEQESDEWEDADEEEKEEDEKKKKSQVKERQSANPLASRLGKLSINENKKKPNQRKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK91</accession>
    <name>G3AK91_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CCR4-Not complex component Not N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_151830</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375065.1">
        <property type="nucleotide sequence ID" value="XM_007375003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK91"/>
    <dbReference type="FunCoup" id="G3AK91">
        <property type="interactions" value="428"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK91"/>
    <dbReference type="GeneID" id="18870909"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_151830"/>
    <dbReference type="eggNOG" id="KOG2150">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020483_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK91"/>
    <dbReference type="OMA" id="VPPGIQD"/>
    <dbReference type="OrthoDB" id="293823at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007207">
        <property type="entry name" value="Not_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04065">
        <property type="entry name" value="Not3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-Not complex component Not N-terminal" evidence="11">
        <location>
            <begin position="3"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="127"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="292"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="390"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="132"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="326"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="347"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="412"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW33550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="713" mass="81444" checksum="307F209A1FEEFCF7" modified="2011-11-16" version="1">MANRKLQKDIEIIFKKIQEGLHEFHYHYDRYESINTDEDSDNQREKEKLEGDLKKEIKRLQKFREQIKNWQSNDVIKTLGLPGNALGVKLNDNKKLIEEAMEIYKDVERSSKLKTFSNQSIMMAFMDSQHGDDDDDDDEDTDEEFLEHIDSSDEDEEEDYADLPSDAIEAIQYLKDTIIQIRDSTQKLNHEYEKLAQKKLRKNNLTTIEAKKEKIQATIENNKFHCKKILKLIRLLKANRVNEFGLIWLLKDDLDEYVSSNGDSNFSSETTLYEDIFNSIVAADQDYSEIYEEKDDDSQTLDSTTNGSILSETNSHPQKLNGHADAPQPRQQSQQSAHPLQQPPQPQLQQQPNSPDLSSPGIVRTLKPAATPSKPVGNLKWAAAAAVGIPETSTTKSRSQEPESNGSHQPDSSPTHRSSVTSSSSPEQAKVQAAAQQQQQQQHQQHQQHQQQQRPLVVENINLDKYKAIVQNSSLPKTEVNLFSDMNLIRVPPGIQDLVISFASKRNHDECKLLVNSTEFNQFTTPIRKKYLPSVVQPLNLSFTPGFRHPTQLMKFQSIWNQIRATYGFEKLVNEIKSLASQDKPDNAALVAELTYVLFYGFYYGLTPAENLIAESYLFDLGWKPYKAQLDNNSSSPLHISSPTVKDDSKSAAPTGSYWFKRIKLISEGTTANPGFEFGDYQVFDLNFWEIFVRYGFKFDYTLCQFNPSTSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKG7</accession>
    <name>G3AKG7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_60269</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374439.1">
        <property type="nucleotide sequence ID" value="XM_007374377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKG7"/>
    <dbReference type="STRING" id="619300.G3AKG7"/>
    <dbReference type="GeneID" id="18873306"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60269"/>
    <dbReference type="HOGENOM" id="CLU_2475119_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKG7"/>
    <dbReference type="OrthoDB" id="5563016at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="88" mass="10309" checksum="E0BCD674CF687A61" modified="2011-11-16" version="1" fragment="single">MEEDENQEEDIMSMVIDSRDLNYLFNVLSEQQRRTIPSHLRYVRQFKDFKSVEISMKKFEDLTNSELVPVSNTATAPILKKKDSSHSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL06</accession>
    <name>G3AL06_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RNA polymerase II fourth largest subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_136337</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic RPB4 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374564.1">
        <property type="nucleotide sequence ID" value="XM_007374502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL06"/>
    <dbReference type="FunCoup" id="G3AL06">
        <property type="interactions" value="239"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL06"/>
    <dbReference type="GeneID" id="18869958"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136337"/>
    <dbReference type="eggNOG" id="KOG2351">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110332_0_0_1"/>
    <dbReference type="InParanoid" id="G3AL06"/>
    <dbReference type="OMA" id="EPLHPFE"/>
    <dbReference type="OrthoDB" id="2186918at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990328">
        <property type="term" value="C:RPB4-RPB7 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045948">
        <property type="term" value="P:positive regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006590">
        <property type="entry name" value="RNA_pol_Rpb4/RPC9_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045222">
        <property type="entry name" value="Rpb4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038324">
        <property type="entry name" value="Rpb4/RPC9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21297">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00657">
        <property type="entry name" value="RPOL4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA polymerase Rpb4/RPC9 core" evidence="5">
        <location>
            <begin position="46"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="70"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="78"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW33049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="203" mass="22562" checksum="653BA65D0AD12801" modified="2011-11-16" version="1">MNVSTSALGVRRRKAKAASQNIDDEENAAILKLGPEFQLTQITNSGEEEQLIALNLSEARLLIRAALKERKNKNKGKQQQAQQSASQSTQSPDQDEDDKEDEISNMELAGPNSNEIMHKTLNYLSNFARFKNSSSTETVEKLLNDFSLSAIEPLHPFELAQLGTLECEDAEEAKSLIPSLANKVSDVQLQTLLTELRKYQTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL59</accession>
    <name>G3AL59_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15 21">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="15">IMP dehydrogenase</shortName>
            <shortName evidence="15">IMPD</shortName>
            <shortName evidence="15">IMPDH</shortName>
            <ecNumber evidence="15 21">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">SPAPADRAFT_60410</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 15">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 15 21">
            <text>IMP + NAD(+) + H2O = XMP + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="15">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="15 21">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer. Seems to be able to form heterotetramers composed from more than 1 of the 3 IMPDH gene products (IMD2-4).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15 20">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="15 21"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374617.1">
        <property type="nucleotide sequence ID" value="XM_007374555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL59"/>
    <dbReference type="FunCoup" id="G3AL59">
        <property type="interactions" value="1261"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL59"/>
    <dbReference type="GeneID" id="18873393"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60410"/>
    <dbReference type="eggNOG" id="KOG2550">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022552_2_1_1"/>
    <dbReference type="InParanoid" id="G3AL59"/>
    <dbReference type="OMA" id="MGYCGAK"/>
    <dbReference type="OrthoDB" id="416622at2759"/>
    <dbReference type="UniPathway" id="UPA00601">
        <property type="reaction ID" value="UER00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04601">
        <property type="entry name" value="CBS_pair_IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000007">
        <property type="entry name" value="Chromosome 19 SCAF14664, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01964">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015875">
        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01302">
        <property type="entry name" value="IMP_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911:SF111">
        <property type="entry name" value="INOSINE-5'-MONOPHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="INOSINE-5-MONOPHOSPHATE DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000130">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01240">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00487">
        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 15 21" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="5 15 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 15 17" id="KW-0520">NAD</keyword>
    <keyword evidence="9 15 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 15 18" id="KW-0630">Potassium</keyword>
    <keyword evidence="7 15 21" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="22">
        <location>
            <begin position="119"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="22">
        <location>
            <begin position="182"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="15 16">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15 16">
        <location>
            <position position="435"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <begin position="276"/>
            <end position="278"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <begin position="326"/>
            <end position="328"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15 18">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15 18">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15 18">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="366"/>
            <end position="368"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="389"/>
            <end position="390"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="413"/>
            <end position="417"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="502"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU003927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU003928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="EGW33102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="521" mass="56236" checksum="6D11C86F8E34EBAA" modified="2011-11-16" version="1">MVLDAASATSYLKNYKEKDGLSVKQLIDSTNFGGLTYNDFLVLPGLINFPSSAVSLDSKLTKKITLRSPFVSSPMDTVTEEAMAIHMALLGGIGILHHNCTPEEQAEMVRKVKKYENGFINDPVVISPDTTVGEVKQMKVELGYSSFPVTETGKIGGKLVGIVTSRDVQFHEQDDDKVSVIMTTDLVVGKQGTTLNEGNELLRTSKKGKLPIVDSNGNLVSLISRTDLQKNQTYPNASKSFDSKQLLCGAAIGTIEADRERLDKLVEAGLDVVVLDSSNGSSVFQLDMIKWIKNKYPDLQVIAGNVVTREQAALLIEAGADGLRIGMGSGSICITQEVMACGRPQGTAVYNVTEFANQFGVPCIADGGIGNIGHITKALALGASCVMMGGLLAGTTETPGNYFYRDGKRLKTYRGMGSIDAMQQTKQNANASTSRYFSESDQVLVAQGVSGAVVDKGSITKFVPYLFNGLQHSLQDIGMKSIEELREKTDSGEVRFEFRTASAQFEGGVHGLHSYEKRLHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL82</accession>
    <name>G3AL82_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peroxisome assembly protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_60436</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in peroxisome biogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxin-22 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374640.1">
        <property type="nucleotide sequence ID" value="XM_007374578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL82"/>
    <dbReference type="GeneID" id="18873407"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60436"/>
    <dbReference type="eggNOG" id="ENOG502S5ME">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1402917_0_0_1"/>
    <dbReference type="InParanoid" id="G3AL82"/>
    <dbReference type="OMA" id="SENMIFI"/>
    <dbReference type="OrthoDB" id="4014227at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11730">
        <property type="entry name" value="Peroxisome assembly protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024359">
        <property type="entry name" value="Peroxin-22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038613">
        <property type="entry name" value="Peroxin-22_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12827">
        <property type="entry name" value="Peroxin-22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="5" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="177" mass="20048" checksum="13DC62480A0E41FF" modified="2011-11-16" version="1">MAKRTQRNPTVWVSALVATSALALTYKLYQTYFKSEEDELETRSIEDSDITSYIISPKYSNKSIAITLSSTFISSKIPLNEILINSENMIFIIPPNLNEEDLNVTIPPNFKLLKCSNVDGYLQVLKNLKPDLLFLCSDDLGINISNLSSDMKLFIKTIVNVDQNKEDIYSKLRPIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALC1</accession>
    <name>G3ALC1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_60473</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374679.1">
        <property type="nucleotide sequence ID" value="XM_007374617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALC1"/>
    <dbReference type="FunCoup" id="G3ALC1">
        <property type="interactions" value="254"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALC1"/>
    <dbReference type="GeneID" id="18873430"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60473"/>
    <dbReference type="eggNOG" id="KOG0594">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="InParanoid" id="G3ALC1"/>
    <dbReference type="OMA" id="SIDITHC"/>
    <dbReference type="OrthoDB" id="413582at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF508">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="7"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW33164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="340" mass="39307" checksum="9794165368AAF122" modified="2011-11-16" version="1">MKLTDLYIDKKQIYSSQISDIYSAKDVKTNTTVCLKIVDVDFSIPPHSIKREIQLVKRLSPHPRIIDYVGGLQIDDDIILVTTLYKHNLSELVKLPRYCKRTTKFDFTNGTHAYIDRNIIDESDIKQWLKQMSCGLKYIHNSGIIHRDIKPSNIMFVNDDITQPIIGDFGISYDVNNDINSGELATEKYIDVCTGIFKSPELLLGITDYLYEIDIWSLAIILTILYSPNFESVLIKQDEELSNDEIECVISDLHLLSNIFKTFGTPSVTDVTSELYWEELTSKTSHFTKFKLIEHNRLDDSQLIPRCEDKSIGFLFRKMTKYDRTTRVTSEEIYSELTNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALD3</accession>
    <name>G3ALD3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_60482</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374691.1">
        <property type="nucleotide sequence ID" value="XM_007374629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALD3"/>
    <dbReference type="GeneID" id="18873436"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60482"/>
    <dbReference type="eggNOG" id="ENOG502SF6T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029128_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALD3"/>
    <dbReference type="OrthoDB" id="4073735at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF22">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN COMPLEX ACID LABILE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="574" mass="67212" checksum="B740EFBA549DD590" modified="2011-11-16" version="1">MTNNNYNEFFSSFLTTYLPRLPDEILMQIFDLFNRSDLWTFLTNFPHHDLRDLVLHHYYSEVAFIVHSEVSWYHRYFLDITQFFNEEIFEFMDTFPENIPRRLIITFDNESFSTIEQFLERYDERIRKVAQLEIALERCQLGDCDLNYLLSFKNLTKIYVQNMNLSNTKIFDNTYWTDHPRLEEIILVQDCVDDWSSFRFPEKLKCLELVQTELDHKLIIPKSVKTLSISGSKLDSLEIFQNQHSCDSTQNLMEDQLPAGLRKLKLETTGIYDFNRIHWPTSLEVLSLAETGLNDSALKIINNLHRWPPGLIDLNISNNPIRLPNFASRLPSNLKILEMNQVELSWLESEHPIEFPETLTELYMNACKIPGLDVLSFPTSLKKLALTTNQIEDVASYDWEILVNLETLDLSGNNITSLDDWMIPPNLINLCLFANPINELSENFPLFIDQYKFNLEYLDLRFCNIKSISVKYIPASLKMLILLGNSLGSNLVLPSSFKSLQHLNLCRCGLATVEFRDEDKQLTTPSPRRKPLDLPAFYSYDWKLCLGKIRKSTNTFHEECLETFRGREPIWLID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALP4</accession>
    <name>G3ALP4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_60620</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374802.1">
        <property type="nucleotide sequence ID" value="XM_007374740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALP4"/>
    <dbReference type="FunCoup" id="G3ALP4">
        <property type="interactions" value="193"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALP4"/>
    <dbReference type="GeneID" id="18873494"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60620"/>
    <dbReference type="eggNOG" id="KOG2914">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_13_0_1"/>
    <dbReference type="InParanoid" id="G3ALP4"/>
    <dbReference type="OMA" id="IWCPHPG"/>
    <dbReference type="OrthoDB" id="40579at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990738">
        <property type="term" value="F:pseudouridine 5'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.240:FF:000001">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901">
        <property type="entry name" value="2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901:SF38">
        <property type="entry name" value="PSEUDOURIDINE-5'-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="229" mass="25944" checksum="A137B02FD40B53A3" modified="2011-11-16" version="1">MDGTLLNTEDLYTEATNELLKLYNKGPLTWDVKIKLQGRPSFESMTIMIEEYDLPLTIEEFQKISMEIQSKLWHKSRFLPGAVELVEHLYNNDVPIALGTSSNTINYHRKTDHLKSEFNYFGKHIVTGDDERIPKGKGKPHPDIWFACLASINEDRISQGLDEIKPEECLVFEDGIPGVISGIAANAHVIWIPDVNALKVLNGEEHNIIGTSGEILTSLTEFNKDKYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALQ6</accession>
    <name>G3ALQ6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">37S ribosomal protein S35, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_60637</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374814.1">
        <property type="nucleotide sequence ID" value="XM_007374752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALQ6"/>
    <dbReference type="FunCoup" id="G3ALQ6">
        <property type="interactions" value="126"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALQ6"/>
    <dbReference type="GeneID" id="18873500"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60637"/>
    <dbReference type="eggNOG" id="ENOG502QVMS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_842157_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALQ6"/>
    <dbReference type="OrthoDB" id="10052321at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021036">
        <property type="entry name" value="Ribosomal_mS45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28158">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S35, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28158:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12298">
        <property type="entry name" value="Bot1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="35754" checksum="B2CFC4B6200C9F79" modified="2011-11-16" version="1">MLARQLTTESSSLVREFSTSSIAHARKSFRKDVSHHKNMVKFFGPKNIVGEYHKNIYYYPNQSNRPNYIDPQTNPVVGQRGTFENTSRSVRSRNPSIHPFPHNVNTKTNLMLSDFLKDEIVRDVRENGLHIQEVSHKYGINQLRVEAVLKLRAVEESFNETSGLDAQEKKDLQHFADVMKRMFPLYQGGNNGDNLTEIPTPKKMLQQRFLTIAESQPFGPLDAAKVLDLEPAVDTLKELSKVKSKEEREKEVSSKKETMIQSYLNLFLKKVKTSGSVMVSLEEIERRIEGLGLIVPVKWFTLYKLLKLVYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALT6</accession>
    <name>G3ALT6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_60052</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374209.1">
        <property type="nucleotide sequence ID" value="XM_007374147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALT6"/>
    <dbReference type="GeneID" id="18873199"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60052"/>
    <dbReference type="HOGENOM" id="CLU_1723475_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALT6"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="152" mass="17108" checksum="B52AEBBAD00C065E" modified="2011-11-16" version="1">MPVKSEFKFVAPAVFNHYESTENSSLTSPSSILSINSGDFGGDDTGIEDAEEFLLEQPSTKKYKPDLLQRQPILMPQYSQYQLQQQQQQQQLPQQILTQAHQIQQSQLQQSPQSQLQQSSAPQELGLFPGDVTTSFDLIFNDVMDDTSLINV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALU2</accession>
    <name>G3ALU2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">mRNA transport regulator MTR2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_54731</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374216.1">
        <property type="nucleotide sequence ID" value="XM_007374154.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALU2"/>
    <dbReference type="FunCoup" id="G3ALU2">
        <property type="interactions" value="167"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALU2"/>
    <dbReference type="GeneID" id="18871881"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54731"/>
    <dbReference type="eggNOG" id="ENOG502RZK7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128326_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALU2"/>
    <dbReference type="OMA" id="FDCHLIP"/>
    <dbReference type="OrthoDB" id="25408at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019488">
        <property type="entry name" value="Nucl_pore_RNA_shuttling_Mtr2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10429">
        <property type="entry name" value="Mtr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="180" mass="20019" checksum="BE77D637BD919C6F" modified="2011-11-16" version="1">MNQQDPTQPIEPFLKDLLASLDAQYASPTQSFPNVEAYATQFASNVKRDCAIIVNGKPIIPTPQEDARLQFQKKWLATPLSSHQLTSFDCHLIPGTGTFIVNFCAKVKFDQSGRNRLGESADLITDNSIGRTVRPIWGSNFGLNVNLVVDESVINNSKGEIISSLNYRFTYIPEDTILKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALX2</accession>
    <name>G3ALX2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_60662</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>myo-inositol(out) + H(+)(out) = myo-inositol(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:60364"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374840.1">
        <property type="nucleotide sequence ID" value="XM_007374778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALX2"/>
    <dbReference type="FunCoup" id="G3ALX2">
        <property type="interactions" value="1254"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALX2"/>
    <dbReference type="GeneID" id="18873511"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60662"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_5_1"/>
    <dbReference type="InParanoid" id="G3ALX2"/>
    <dbReference type="OMA" id="ETGWRWM"/>
    <dbReference type="OrthoDB" id="6339427at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005366">
        <property type="term" value="F:myo-inositol:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904679">
        <property type="term" value="P:myo-inositol import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000073">
        <property type="entry name" value="MFS myo-inositol transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF12">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="319"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="355"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="425"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="464"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="496"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="71"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW33325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="558" mass="60278" checksum="61E404A4F04EA7BF" modified="2011-11-16" version="1">MVQQNIQNKDTKQNGRDTPSVMDYVVKSSASSIKSGTSEEMLLVGPSSVVDATSFDISDDDVKPTKTVLILTLASSISGFMFGYDTGYISSALIQVGTDLSNKILTSGEKELITSATSLGALLGAIVGGILANLIGRRMVLLGSNVIFVVGTIIQLVAHTVWTMIAGRFVLGWGVGIGSFIAPLMLSELAPAKYRGRLIVTNVMFITGGQLIAYLINWGLTRVEHGWRVSVGLCMIPPVFQFVLFWFLPDTPRFYVMNGEVEKATDILKKIHRDPSDEFIQATIEEMVSSNSNVPGRGPLQKSWESIKIIHKTPGNLRALILACGLQGIQQFTGFNSLMYFSATIFETIGFNNPTAVSIIVAATNFVFTGIAICIIDIVGRRRILLFGIPCMCISLVVCAIAFHFLNVDFSSGSGVIESSGISGWGIVVIVSMVAFVASYAIGIGNSAWVGVELFSDVNVRSVGAMYAAGTNWAGSMVIAATFLTMLQNITPTGTFSFFAGLCFVSFLFVYFLLPETAGLELEETTSFLSDGFNVKQASRMARERRLHSKFANKTRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM33</accession>
    <name>G3AM33_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <ecNumber evidence="8">1.1.1.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SOL1</name>
        <name evidence="8" type="ORF">SPAPADRAFT_137270</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May be involved in regulation of tRNA subcellular distribution.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="8"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374253.1">
        <property type="nucleotide sequence ID" value="XM_007374191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM33"/>
    <dbReference type="FunCoup" id="G3AM33">
        <property type="interactions" value="296"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM33"/>
    <dbReference type="GeneID" id="18870032"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137270"/>
    <dbReference type="eggNOG" id="KOG3147">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053947_0_1_1"/>
    <dbReference type="InParanoid" id="G3AM33"/>
    <dbReference type="OMA" id="KLAWCLP"/>
    <dbReference type="OrthoDB" id="432544at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01400">
        <property type="entry name" value="6PGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000017">
        <property type="entry name" value="6-phosphogluconolactonase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005900">
        <property type="entry name" value="6-phosphogluconolactonase_DevB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039104">
        <property type="entry name" value="6PGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01198">
        <property type="entry name" value="pgl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054:SF0">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="7">
        <location>
            <begin position="96"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="56"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="332" mass="37024" checksum="38E61F5934D5F47B" modified="2011-11-16" version="1">MTTTVPLIYSFPEFLGVADAVADHVISAQNQTLYNTTDPKQIELIKSRPALLKSSSGISVTQQSPSHSPSQSQNGISNNVTNGGSKAKKDKKIHKKQEVRFKIAISGGSLIKILHQGLLPRQEYIEWDKWDIYFADERLVPFDSPDSNYGQAKREIFDLIEGDKKPNIYHIDEELIDDNQEAADDYEKQLIRNFAKKDSVKLPMFDLLLLGCAPDGHIASLFPNFGEQLREKLAWCLPVENAPSGPKNRITLSIPVICHAARVTFVVEGKTKAPIIRTIMERPEKGLPSSIVNEGAAGRVSWFVDDAALNDLYDITKKKYKFLSFPEPATAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM97</accession>
    <name>G3AM97_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_151428</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat G protein beta family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374910.1">
        <property type="nucleotide sequence ID" value="XM_007374848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM97"/>
    <dbReference type="FunCoup" id="G3AM97">
        <property type="interactions" value="452"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM97"/>
    <dbReference type="GeneID" id="18870893"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_151428"/>
    <dbReference type="eggNOG" id="KOG0286">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_34_1"/>
    <dbReference type="InParanoid" id="G3AM97"/>
    <dbReference type="OMA" id="PLDSQWV"/>
    <dbReference type="OrthoDB" id="10255630at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016346">
        <property type="entry name" value="G-protein_beta_1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19850">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN BETA G PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25391">
        <property type="entry name" value="WD40_Gbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002394">
        <property type="entry name" value="GN-bd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="60"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="164"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="205"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="251"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="371"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW33395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="404" mass="44643" checksum="132E4C31E3E6EA5B" modified="2011-11-16" version="1">MDRNTSLADQIKAAQYQARTLYNDILRLKTHTQNITMQSAARVIAPIPRNSCTLKLYNTLKGHQNKVAKLVWSADSSKILSASQDGYMFIWDPITGFKKHVIQLENTWVLTCSYSADEKLAASGGLDNVCTIYKIKPDTITNPISYRGGNQPNQGIYQKVESVFKGHTAYISECEFIDNSSIVTASGDMTCILWDVTKGRKVRDFIDHVSDILSLAIFPQSIFNANVFISGSADGYAKIWDLRSPTPSQNFFISNSDVNSVKAFPGGGAFITGSDDGLVRLFDMRSDCELSTYNLLAQLQTPGGKISQTFLTRLDDSEWMNSNTSINSAIENQGIYSLDFGKSGRFIYACYSEQGCIVWDTLKNEIVDSVGNDHNNKINQVSVSPDGVALATGSWDTTIKVWSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMU5</accession>
    <name>G3AMU5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_138624</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375635.1">
        <property type="nucleotide sequence ID" value="XM_007375573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMU5"/>
    <dbReference type="GeneID" id="18870136"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138624"/>
    <dbReference type="eggNOG" id="ENOG502RPUT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_715708_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMU5"/>
    <dbReference type="OMA" id="YERIVWR"/>
    <dbReference type="OrthoDB" id="4005225at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="399" mass="46088" checksum="3505677CE019FC15" modified="2011-11-16" version="1">MLGSGKPSENMVLAEALNLLQRSRDQDQEVNSTNYKPSLYDNIVKGPAQEQYSDLIRTPKIENRKQKQRISDQFISNLLSGIKSSEVSDVAGALDFADLYNMNSRELSLYIKSVKSEQKLFEILETFYYNDRLTFNVLSSIVLNKALVNIDKAPIDLVNIKNNRLLKLDPLGITMFNIVLLGKYHSLHQPMTVLRNLKQNFNDVYLPLIKKHKLSAFYERIVWRYTFEFLKQFDELHYLQEINRVKSGFVILEATTKSSTPIAKYMLETYGESLTELQTIFLKIFTNPYIEQRIIKELESPGRSKTCIALQKLSVKHHMCTLSQIPKDQWSRRACYALVADLEQFIVESLEISNDKELQGLGNELKTYHMKIITSQQSEISESKEALDDFFNVLQVKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN88</accession>
    <name>G3AN88_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein TYE7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TYE7</name>
        <name evidence="3" type="ORF">SPAPADRAFT_61540</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375747.1">
        <property type="nucleotide sequence ID" value="XM_007375685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN88"/>
    <dbReference type="STRING" id="619300.G3AN88"/>
    <dbReference type="GeneID" id="18873942"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61540"/>
    <dbReference type="eggNOG" id="KOG2588">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067895_1_0_1"/>
    <dbReference type="InParanoid" id="G3AN88"/>
    <dbReference type="OMA" id="SQKKAHN"/>
    <dbReference type="OrthoDB" id="2133190at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11395">
        <property type="entry name" value="bHLHzip_SREBP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052099">
        <property type="entry name" value="Regulatory_TF_Diverse"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47336:SF3">
        <property type="entry name" value="SERINE-RICH PROTEIN TYE7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47336">
        <property type="entry name" value="TRANSCRIPTION FACTOR HMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="232"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="165"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="326" mass="36724" checksum="586E70FA8CE404FB" modified="2011-11-16" version="1">MDYKFNNISPPMHYHTNYNLGFMNHECYSVPLSPVNNSNEPNEWMDDFLINSAPMTTVNSGSGLNNTPLQPSNLGNQPINFTQYFEEGSIFHPVSSSAETSYANSPENVYPEKVDTKTNNNISEPKRNKQNLNLETLEVLFNTKDILKQEPTDTLLPKRKRNATPVESSVPVSSNESSPMLSKTNTISLSPASSMGSSTSSSEKIEESIKTEKKSQPRTPKKRAPRKKLTDSQKKAHNKIEKRYRININAKIAGIQKIIPWVAYEKTAFETGEQASDIDANCARLNKSMILEKATDYILYLQQKEIQMTEENKRLKEQVLSLGGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANB0</accession>
    <name>G3ANB0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_138574</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375769.1">
        <property type="nucleotide sequence ID" value="XM_007375707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANB0"/>
    <dbReference type="STRING" id="619300.G3ANB0"/>
    <dbReference type="GeneID" id="18870130"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138574"/>
    <dbReference type="eggNOG" id="ENOG502S1X9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_364501_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANB0"/>
    <dbReference type="OMA" id="NLWWCCY"/>
    <dbReference type="OrthoDB" id="10031947at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050797">
        <property type="entry name" value="Carb_Metab_Trans_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="6">
        <location>
            <begin position="350"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="61"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="631"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="66"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="636"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="654"/>
            <end position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="759" mass="88142" checksum="73CD4849AB508C6C" modified="2011-11-16" version="1">MFVFPAFDTNYKTRKRTFKCCQNCRIKRTKCEITSTDYEALGCLNCRRHKFKCSLIKKKAHPTDEPQTQPQQQPLQQVPQSQSQPQLQYQQISQSPVDDGNNRKYMTQITPQYLHDTFNFNTSTRSDTTFQYLFHGHPKVIISSQDKDQTIYHESGVYVKTKKEDDKKSYKIKHFGSDTEKHFNIKSEKVYQFLLSINAFTLSSPDYPISKEDEVSLLKLYFYKTNSIFPLVQENFFWKEYNENKAQNTLIYGMVLAISRDKLAEPILKRIFSLGSIARTGHPILEMNQEQYNTELVKFMSDLEFKIRQLMLILVQLGDEDKFSRIAVHLLLSLHFGYDRLGNEQSAHDLMDAIGIGTAIGIHMKRLPQRSAEFIEYSTNLWWCCYVFDRFNGLVNARPCFIKSEDFNVDLPYSNITLLKMVQIARNLENMFFAVFQPFNNNNIPTNNSMARYSKFNIDEFQKIEFELCEQERSSGRQIYSIGKTSDKHQDYILDSTHLLTRVVNNVVILASQKGKYDNPEIPNHIPEAIALRASSNMLWYLSNMPDEYLIQLPMIPWCMSLGMAVALKKYARQHVTKYNFSEYDQYHDEYEFESYVSYLEKFSATWYVVDEICRLTRDFTDKLEKKMLRKKKRDAARAKEEATIKKAKKEGENSQSPQSTNRLSVVPEQDQLQTSSTVPPIPIIHTNSSNVSAGVDSNIGESPFMHPDAPFDPASTGSVGSISSDTLDQYDQYFESMQIDIFNNDFFKDVPNVINLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANH3</accession>
    <name>G3ANH3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Agglutinin-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_152224</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ALS family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375238.1">
        <property type="nucleotide sequence ID" value="XM_007375176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANH3"/>
    <dbReference type="FunCoup" id="G3ANH3">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="GeneID" id="18870920"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_152224"/>
    <dbReference type="eggNOG" id="ENOG502RGCG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031316_2_0_1"/>
    <dbReference type="InParanoid" id="G3ANH3"/>
    <dbReference type="OMA" id="CTDKTKP"/>
    <dbReference type="OrthoDB" id="3981162at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903561">
        <property type="term" value="C:extracellular vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030446">
        <property type="term" value="C:hyphal cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030445">
        <property type="term" value="C:yeast-form cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044403">
        <property type="term" value="P:biological process involved in symbiotic interaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030448">
        <property type="term" value="P:hyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2430">
        <property type="entry name" value="Agglutinin-like protein, N-terminal domain, N2 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008966">
        <property type="entry name" value="Adhesion_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008440">
        <property type="entry name" value="Agglutinin-like_ALS_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024672">
        <property type="entry name" value="Agglutinin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043063">
        <property type="entry name" value="Agglutinin-like_N_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033504">
        <property type="entry name" value="ALS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011252">
        <property type="entry name" value="Fibrogen-bd_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793:SF2">
        <property type="entry name" value="AGGLUTININ-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793">
        <property type="entry name" value="ALPHA-AGGLUTININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05792">
        <property type="entry name" value="Candida_ALS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11766">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01056">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49401">
        <property type="entry name" value="Bacterial adhesins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0134">Cell wall</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 17" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0843">Virulence</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442527" description="Agglutinin-like protein N-terminal domain-containing protein" evidence="17">
        <location>
            <begin position="18"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Agglutinin-like protein N-terminal" evidence="18">
        <location>
            <begin position="53"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="436"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="437"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="19">
        <location>
            <position position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW31962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="483" mass="51991" checksum="30C2E4E783CF8F76" modified="2011-11-16" version="1" fragment="single">MLKFAVFTILALATATAEQVSGIFTSFDSLEWEKAANYGYASPAYPSWIATLSWAIKGSKVSAGDTFTLTLPCVFKFTTTQTSVNLNVGSTNYATCTFNPGDIVVAFSKLDCVMLDTVTSSLDAHGSINFPVAFNVGGSALSTDLEDSTCFADGENTVSFFDGDNKLSTQAIFSGGSTDDPEKIVYGNRVVPSLNKQQHFLLGGNCPKGYSSGSIGLEIKNSGPKFDCDSIHMKITNSLNAWFLPENADDDFDFTYTCTSTSFVVDYKNIPAGYRPFIDTLVDIANGQSITLNYINNYICDGSKTTTNNGKIIAWAAYENNVAGGNGEEVKVVTSTYTGSTTEVSTMPFETSKDKTITIVVNVPVPTVTTTTTYVGISTSYTTESALPGSTASVIEYEPIHTTTTITTCWNQQSTFTSIYSTDTWPTDTELVIEPCESSSERTSSTDSIKPSKTHTSCTDKTKPSKPHQTTSCTDKTKPSKPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANJ7</accession>
    <name>G3ANJ7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_67130</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375802.1">
        <property type="nucleotide sequence ID" value="XM_007375740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANJ7"/>
    <dbReference type="GeneID" id="18875141"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67130"/>
    <dbReference type="HOGENOM" id="CLU_057903_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANJ7"/>
    <dbReference type="OrthoDB" id="4016968at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="26"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="429" mass="50194" checksum="3C3A523D1500886C" modified="2011-11-16" version="1">MQRSLRKVGQYNEMQSPMDSSPQSGKSSLLCLPVEILYEIVCYLNQCNLLQVSLINKLLRDVAFRKLYSRIYVQTSSVRQLVVGDYVNTPLHINYTLVNGREKLISLMKHTYSLRFLREILFHGDDNGIAMGLSRAMRDFLDGVNIYLCYNSMGEVYSREPYLYRNLVIIRDNQFIFDEDNYSIQSLTLLASTPNQDYNQDLSFVYRLKSLKKLSLTIQTKDTLKLLRYKLPEKLHIEQLSLNFRNLLGINVSALVTLFDLCNVTSLYIRIPQGYYKFLPTFVVPEIIHLLSQTINVRNFGLSNSAPINYMKVLRAIRKNTLNTLYLEPWYGHEFSHSPKKLLKILHEQQLTLTRIFYGKNFNAHGFEVFEEVFRVQYGQHRLERVLNKLRREVSSRKRLPCLKQIVLDQMHYFTVTTANGNMNFKSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANM1</accession>
    <name>G3ANM1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_139731</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375826.1">
        <property type="nucleotide sequence ID" value="XM_007375764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANM1"/>
    <dbReference type="FunCoup" id="G3ANM1">
        <property type="interactions" value="876"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANM1"/>
    <dbReference type="GeneID" id="18870232"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139731"/>
    <dbReference type="eggNOG" id="KOG0282">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022571_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANM1"/>
    <dbReference type="OMA" id="AMWDVEA"/>
    <dbReference type="OrthoDB" id="10257301at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000386">
        <property type="term" value="F:second spliceosomal transesterification activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000389">
        <property type="term" value="P:mRNA 3'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000348">
        <property type="term" value="P:mRNA branch site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032847">
        <property type="entry name" value="PRPF17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43979">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43979:SF1">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="149"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="192"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="272"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="46"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="436" mass="48965" checksum="4F1240428011AC59" modified="2011-11-16" version="1">MAIVNYESDSDNSNHSDVEAVVTKKQRIDSGKKLHIDETTFNYNLLSQERRVNASAKQRAKELKKKRKGGPWGGYSDDEEEQEHQEEPESESESEPEPEPAPTQEEPSTVTTKFVGPNQFDYLGRTYMHAPSLPTPTTCFTPKTIIHSFAAHDHGINKLALLPKSGHLLLSCGNDSLIKLWSVETHQLLRIFMGHSASVKDIVFNFDGSKFLSCGYDRLVNLWNTETGEILHSFKLSSLPQALLFHGQEFLVGLSNNKIEHYNLQGTLLQTYDHHQGGINTLVHITNNIFMSTSDDKTVRMWNYGINIPIKTISSHLQHAMPAGAVHPTANYIALQSMDNTIRVIHSTGKYKYKKSKTFTGHHCSGYGIDIAISQDGRIIMSGDSKGYAYFWDWKTGNLVNRIKCSDKVVKCIVMHPKQKSMVYVGGESGEVYICD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANM7</accession>
    <name>G3ANM7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_139333</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375832.1">
        <property type="nucleotide sequence ID" value="XM_007375770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANM7"/>
    <dbReference type="GeneID" id="18870200"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139333"/>
    <dbReference type="eggNOG" id="ENOG502S1A5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081479_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANM7"/>
    <dbReference type="OMA" id="YINHIAN"/>
    <dbReference type="OrthoDB" id="4096434at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="276" mass="32720" checksum="F647DD080C3E3FA7" modified="2011-11-16" version="1">MHNYLGSGDSSKKGCNKNDADYLNYEQDQDEKERALTNLRSDIYSIGPKILPLPFSREFMNLKYTKFVPLAQCKLDPKYALRGGARTNYNTTKRFDIHQPTEPDDEAKLGLCRLKYESGSEFLCLDTETDVDAEKTKHKSRSRRSKKEMKEIEKLAEVRTDRYYSRLNIYELSKILELDGFHISLTKEIELKILELFGNYCDFRLGYQTWIRDTNRAKRNDLINQLYSYSSVFYPEIDKFKLEVIVRRGSYSLMQTRLRRERRMSKHINRSRYKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANS7</accession>
    <name>G3ANS7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Homoserine kinase</fullName>
            <ecNumber evidence="3">2.7.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_61115</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Commits homoserine to the threonine biosynthesis pathway by catalyzing its O-phosphorylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-homoserine + ATP = O-phospho-L-homoserine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.39"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:13986"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GHMP kinase family. Homoserine kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.39" evidence="3"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375288.1">
        <property type="nucleotide sequence ID" value="XM_007375226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANS7"/>
    <dbReference type="FunCoup" id="G3ANS7">
        <property type="interactions" value="184"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANS7"/>
    <dbReference type="GeneID" id="18873720"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61115"/>
    <dbReference type="eggNOG" id="KOG1537">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041243_2_1_1"/>
    <dbReference type="InParanoid" id="G3ANS7"/>
    <dbReference type="OMA" id="CANRIPH"/>
    <dbReference type="OrthoDB" id="195231at2759"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00064"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004413">
        <property type="term" value="F:homoserine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000068">
        <property type="entry name" value="Homoserine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00384">
        <property type="entry name" value="Homoser_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000870">
        <property type="entry name" value="Homoserine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00191">
        <property type="entry name" value="thrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF1">
        <property type="entry name" value="HOMOSERINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861">
        <property type="entry name" value="HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000676">
        <property type="entry name" value="Homoser_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00958">
        <property type="entry name" value="HOMSERKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0791">Threonine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="13">
        <location>
            <begin position="72"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW32012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="355" mass="38722" checksum="39DE2EE0A2A22FBD" modified="2011-11-16" version="1">MSSQIRRFKIKVPASSANIGPGFDVLGIGLQLYLTLDVTIDPSRSSNSDPNNVVLKYEGDLAENVPLQSDKNLVTSSALYVLRCNGFTQFPNGTFIDVTNPVPLGRGLGSSASAIVAGVCLGNEIAQLGMDKIRMLDYCLMIERHPDNIAAAMLGGFVGSYLNELPIEEHDANLPLEYILPRSSTPVDKIVNSPPPKNIGEYMSYDWCKSIKCVCIIPNFEVSTDKSRAVLPESYTRPDIVYNLQRIAILTTALTQTPPNEKLIYQAMKDKIHQPYRSGLIPGLPEVLQSVTPESHPGLCGICLSGAGPTILCLATKNYDEIAKTVVDIFKRDGVECDWKVLDLAYDGATVEQIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANU7</accession>
    <name>G3ANU7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">YAP1 binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61643</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375848.1">
        <property type="nucleotide sequence ID" value="XM_007375786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANU7"/>
    <dbReference type="FunCoup" id="G3ANU7">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANU7"/>
    <dbReference type="GeneID" id="18873984"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61643"/>
    <dbReference type="eggNOG" id="ENOG502RY9F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024514_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANU7"/>
    <dbReference type="OMA" id="TYEIGWD"/>
    <dbReference type="OrthoDB" id="5396786at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013877">
        <property type="entry name" value="YAP-bd/ALF4/Glomulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040347">
        <property type="entry name" value="YBP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28020">
        <property type="entry name" value="YAP1-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28020:SF1">
        <property type="entry name" value="YAP1-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08568">
        <property type="entry name" value="Kinetochor_Ybp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="544"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="544"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="693" mass="80029" checksum="CAAB927263C96727" modified="2011-11-16" version="1">MSETDVSSDTTIEPFVFEKVLENLEAGCRDAIATKDYLSYSTLLDIYLSEPSKYTNEEKEKLLQSILDILQEHPDLTYKIGWDLPSLLLPYVDSDYQFNKGIRNSPCVYKILKSFECLARHGNPKELFLKCCELLSTIKVTDSPSTIKPEFQENFFDIKLYCIFELIDACLKRIQTLYPSRFLSMTVTSYINLAYVNLVRGQWSATNAHFILKRAYSFARNYINPPLPDQIDPELTKEELAKITEDEEYLQRKLLTGFLTQLVSYTSKLGAEGYSIDMFSWLQNKSKKSKLTFHFQIDPVLYDRLVELAYSYDIPLRDDFKKFIADSNAIFKEFEFKDDTDEDDLSGEIFERLVVDYQKNLFTSIIDSDSKSINDSIIGELILHTHVVCTKKSFDHVDVSIYDAVVMGLRILIPQMVQPSFVNRGAFDVAVFWSWYAIYQTGLKRKKPEVEFAAIPKLLLRLYMQSILFTIIHCKEQPNHRFMLFTLLSKILCLAPENTGYEFIKDTLENCPYEATKAPVIGVYKELLLKDKFEPVDNLEEEFSKASLDDKNRENDKEQNGDKKEDKPIVPPPLPRRSTGKSHKFFTFTEERLDDLLTLILFAQSSTFIANEDKSVSIDPATLSTLAAYLNLLIVLKKDKILLENKDKLDRVLRVVENNIDAVQTKHKNNPEANQFEFNAAGMLELTIERFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANW3</accession>
    <name>G3ANW3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Myosin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_138259</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375864.1">
        <property type="nucleotide sequence ID" value="XM_007375802.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3ANW3">
        <property type="interactions" value="629"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANW3"/>
    <dbReference type="GeneID" id="18870109"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138259"/>
    <dbReference type="eggNOG" id="KOG0160">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000192_3_1_1"/>
    <dbReference type="InParanoid" id="G3ANW3"/>
    <dbReference type="OMA" id="EIMFDDR"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15480">
        <property type="entry name" value="fMyo2p_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01380">
        <property type="entry name" value="MYSc_Myo5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046943">
        <property type="entry name" value="Fungal_Myo2/2A_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036103">
        <property type="entry name" value="MYSc_Myo5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF706">
        <property type="entry name" value="DILUTE CLASS UNCONVENTIONAL MYOSIN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50084">
        <property type="entry name" value="Myosin S1 fragment, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 9" id="KW-0518">Myosin</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="13">
        <location>
            <begin position="6"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="12">
        <location>
            <begin position="73"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="domain" description="Dilute" evidence="11">
        <location>
            <begin position="1217"/>
            <end position="1492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="9">
        <location>
            <begin position="652"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="955"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="167"/>
            <end position="174"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW32588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1579" mass="181653" checksum="4F7B8E32B7BF37B8" modified="2011-11-16" version="1">MVSLYDVGTRCWYPDEKLGWVGTTVKSNTQDNNKYIIELTLEDDESQIFTIETENLSEDNPELPPLRNPPILEAAEDLTSLSYLNEPAVLQAIKLRYSQLNIYTYSGIVLIATNPFQRVDQYYSHDIVQAYSGKRRGELDPHLFAIAEEAFRCMKDDGENQTIVVSGESGAGKTVSAKYIMRYFATVEEDFQGSTIDHKADMSDVEKQILATNPIMEAFGNAKTTRNDNSSRFGKYLEILFDTDVSIIGARIRTYLLERSRLVFQPQSERNYHIFYQLLAGMSEDEKQTLGLTKPEDFKYTNQGGAPQIDGVDDAAEFSITRDALQLIGIDSDKQFEIFKILAGLLHIGNIDIAATRNDAYLSADEPNLVKACDLLGIDANAFAKWCVKKQITTRSEKIISNLSHQQALVARDSFTKYIYSSMFDWLVDYVNNDLCPDEVTAKINSFIGVLDIYGFEHFEKNSFEQFCINYANEKLQQEFNQHVFKLEQEEYIKEEIEWSFIEFSDNQPCIDLIENKLGILALLDEESRLPSGNDQSWIEKMYQNLNKEPTNKVFKKPRFGQSKFIVSHYALDVSYDSEGFIEKNRDTVGEGHLDVMKNTTNPLLQDILSIVEKNAAAALTASASSAESERSRPTKTANKKITLGSMFKNSLIDLMKTIHSTNVHYIRCIKPNEHKTAWEFDSLMVLSQLRACGVLETIRISCAGYPSRWTYSEFADRYHILLPSKDWIKVMSGETTSDEAINELCNQILDKYIEDKLKYQLGNSKIFFKAGMLAHFEKLRSDKLYQSAVMIQKHLRGRYYRKQYIMTRQSHIRLQSLIRGYMVRDRVEKERQCNAATKIQTLIRAYLARKQYVNTVNSVITLQKSIRGLQARRNYLSLRTEASTITIQNAWRGYQERTKYNKLKKSTVVVQSAIRRQYAIRQLKELKVEAKSVHKLQEVSYQLENKVIDLTQSLTSKIQENKKLLEEIEQLKSLMVQHDEAQESLKTRELEYQQQVDSLNGEHLQEISNLKQELANINSEYASAEAKIKQLSQEQADLREEVHRTIEELNKAKEDLVKRDTIEVDLKTHIEQLKSELAQLQSQQQVSKSRNGSAAVINNPKTRTAVNNKRYSSASAWSTNNNLDNVNRPVSVIAVSNDDYADIDDINDELFRLLRDSRHLHREIVDGLLKGLKIPDASIAADLTRKEVLFPSRIIIIILSDMWRLGLTKESEEFLGEVLSSIQHIVSLLKDDDVIPNGAFWLSNTHELYSFVSYAQQTIIDNDTLSHEMSEEEFEEYLKLVAVVKEDFESLSYNIYNMWMKKMEKELEKKAISAVVLSQSLPGFMAPENSPFLSKVFSSGTQYKMDDILSLFNSVYWAMKSYFIEHEVINEVIIELLRFLDGLCFNDLIMRRNFLSWKRGLQLNYNVTRLEEWCKSHEIQEGSVYLSHLLQAAKLLQLRKNTPEDIGIIYEICYTLKPIQIQKLISQYLVADYETPIAPNVLQLVADRVRESNGNSDELFEMVNTDGHFNDPFRTIDLRPFSRVEAYVPAWLNLPVIRRIVELVAKNASVQETQSLQQQLEEEDEYHEQPQTVMVNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP01</accession>
    <name>G3AP01_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">U1 small nuclear ribonucleoprotein component SNU71</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_61131</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the U1 snRNP particle, which recognizes and binds the 5'-splice site of pre-mRNA. Together with other non-snRNP factors, U1 snRNP forms the spliceosomal commitment complex, that targets pre-mRNA to the splicing pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNU71 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375308.1">
        <property type="nucleotide sequence ID" value="XM_007375246.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP01"/>
    <dbReference type="STRING" id="619300.G3AP01"/>
    <dbReference type="GeneID" id="18873732"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61131"/>
    <dbReference type="eggNOG" id="KOG2146">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095039_1_1_1"/>
    <dbReference type="InParanoid" id="G3AP01"/>
    <dbReference type="OMA" id="DRDESIY"/>
    <dbReference type="OrthoDB" id="163257at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1390.10">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002483">
        <property type="entry name" value="PWI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036483">
        <property type="entry name" value="PWI_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01480">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101233">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51025">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="PWI" evidence="7">
        <location>
            <begin position="23"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="118"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="118"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="193"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="215"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="236" mass="28191" checksum="73594F7F772DE980" modified="2011-11-16" version="1">MAYRLHEVNEDFIKDKLDKQKSSSKFPKIFKTNVDITKITNWPAIKKWIQHRIDQLIPDDEIFVEYVYELIRSDSPCPDIKAIQLQVDDFLGKEDSLEFCTQLWQLLIDSEHGKLKDNKQVKEQPVKEQPVKEQPVKEQSTKEQSDRKEEKQVQEQHEAKIQSPKKTKTNYNRSSSYTSKSNESTNKTSYRSQNDRDYRRDRDLPTYKANTHGQRQRESPDERYRDERRIRARKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP85</accession>
    <name>G3AP85_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_72018</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375932.1">
        <property type="nucleotide sequence ID" value="XM_007375870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP85"/>
    <dbReference type="FunCoup" id="G3AP85">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP85"/>
    <dbReference type="GeneID" id="18875407"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72018"/>
    <dbReference type="eggNOG" id="ENOG502QVVM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004624_8_2_1"/>
    <dbReference type="InParanoid" id="G3AP85"/>
    <dbReference type="OMA" id="KWYDEVL"/>
    <dbReference type="OrthoDB" id="1887033at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046557">
        <property type="term" value="F:glucan endo-1,6-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000100">
        <property type="entry name" value="Glycoside hydrolase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF43">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="500" mass="57064" checksum="C98FF1F97693B4E4" modified="2011-11-16" version="1">MFSKLKLSHKGGKVEAADPAATPGQPPSTSQIYQSRKNYGVNFGACFVLEKWIYHDLFINDSGVDCELKAVKDLVDKYGEEEARNRFENHWNEYANDDDWNWLVDHGANSIRLPIGYWDIDGGAYTSGFKFEKYKHVYANAWSIIKEKYIEVAAKHNISVIVDIHGLPYGANKSDHSGEPGESKFWDSESAQLQMAKAVGFVAQDLSKYENIAGIQIVNEADFTDSTKKRSKYYSAAINEIRSHDKKVPIVISDGWWTDQWVKWVQEQQNDLGQNIGVVIDHHCYRCFDDKDKSKEPQQIINDLQNDLLTNLSEGGKYVDIMVGEYSCVLDTASWDKIGNHNRDGLVCDYGRRQGDLMQERTCGTYFWTFKFQSGNGGEWDFKTMTEKGALSPPPKIEHAPGQSEFDQALESAFEGHKNYWHSANPKEKYDHDRFKDGFTTAWADADAFAKFHGSRIGRKQAWKQARLQEHVHAKGKSNFLWEWESGFDQGLAGYYTHCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3APP3</accession>
    <name>G3APP3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_50787</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375490.1">
        <property type="nucleotide sequence ID" value="XM_007375428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APP3"/>
    <dbReference type="FunCoup" id="G3APP3">
        <property type="interactions" value="185"/>
    </dbReference>
    <dbReference type="GeneID" id="18871484"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50787"/>
    <dbReference type="eggNOG" id="KOG1470">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016665_2_1_1"/>
    <dbReference type="InParanoid" id="G3APP3"/>
    <dbReference type="OMA" id="TIEWVRL"/>
    <dbReference type="OrthoDB" id="43460at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052432">
        <property type="entry name" value="PITP/CRAL-TRIO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46590:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN CSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46590">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN CSR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="383"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="622" mass="72230" checksum="5F77EFEF162E8EBE" modified="2011-11-16" version="1">MTSSVAGPKYRPARIQTLTADQEIVLKQVWAYFFKYFGYPLDIDKSDLPFKECFIASTSTQHFDESHTLHPLIYVPTRTAASITSRKTTGSTRSRRSMFSGRRRRKSESFSVEGVKPPRSRLPHNSKRMVQIQTQSSFERYVPVDVASDEFYYTYAHHYKRAFEYSDDYKNLVDADGQYIGSVEEETSSQVDELEEDGDMAGSVYSLETFVTAASSFTDEMPVHKPKKNPSIKAPKRAVYNLYNGGRIKPPGKFPHPSFITMKINNKITVQPVDQFKRTMNMHHTVRHDRSLLSCFRRYEIQSIHTALNTLLRHDLLDNFVLRFIRARKWDTEKAIAMLTKTLDWRINEFPADNWVMEGDAPSYLKGINQGFVKNFTKEKSWIKGRDKNNNPIFTFQARKHLTTDASVKQNQRYAVVMIEWARLILKDVSESVDTFTILFDLTGFSLKNADYSTIKFLADCLEAHYPETLGFILIHNAPWIFASVWNIIKHWIDPLVAEKIHFTKDLNELTRFIDIKAIPDYLGGQDPTRGRYPIPTARDAFPPKRKDAEYIRLMKERDQLYCRFYETTKKWIESTNPAISELYLKDKLALDIELAKNYVLLDPYIRNPGLYDRDGTLTMSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3APP4</accession>
    <name>G3APP4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_61296</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the UTP23/FCF1 family. FCF1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375491.1">
        <property type="nucleotide sequence ID" value="XM_007375429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APP4"/>
    <dbReference type="FunCoup" id="G3APP4">
        <property type="interactions" value="951"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APP4"/>
    <dbReference type="GeneID" id="18873827"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61296"/>
    <dbReference type="eggNOG" id="KOG3165">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081098_0_1_1"/>
    <dbReference type="InParanoid" id="G3APP4"/>
    <dbReference type="OMA" id="GMMDCLL"/>
    <dbReference type="OrthoDB" id="76105at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09864">
        <property type="entry name" value="PIN_Fcf1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000004">
        <property type="entry name" value="rRNA-processing protein FCF1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006984">
        <property type="entry name" value="Fcf1/UTP23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037503">
        <property type="entry name" value="Fcf1_PIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04900">
        <property type="entry name" value="Fcf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="PIN" evidence="7">
        <location>
            <begin position="65"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="191" mass="21620" checksum="242F2F449A71ABC5" modified="2011-11-16" version="1">MGKAKKTRKFALVKRTLNKKDARMTKSNSTTNKKSSQDDPELTRSVPQVSSALFFKYNEAIKPPYQVLIDTNFINFSIQKKIDIVRGLMDCLMAKCIPIITDCVMAELEKLGPKYRIALKLAKDPRIQRLSCTHGGTYADDCLVNRVMQHKCYIVATNDADLKRRVRKVPGIPLMSVGGHAYVIERLPDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3APQ5</accession>
    <name>G3APQ5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Guanylate kinase</fullName>
            <ecNumber evidence="2">2.7.4.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">GMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_50797</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the guanylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.8" evidence="2"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375502.1">
        <property type="nucleotide sequence ID" value="XM_007375440.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APQ5"/>
    <dbReference type="FunCoup" id="G3APQ5">
        <property type="interactions" value="565"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APQ5"/>
    <dbReference type="GeneID" id="18871486"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50797"/>
    <dbReference type="eggNOG" id="KOG0707">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001715_0_4_1"/>
    <dbReference type="InParanoid" id="G3APQ5"/>
    <dbReference type="OMA" id="NFITHEV"/>
    <dbReference type="OrthoDB" id="6334211at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004385">
        <property type="term" value="F:GMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000002">
        <property type="entry name" value="Guanylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000776">
        <property type="entry name" value="Guanylate kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.10">
        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00328">
        <property type="entry name" value="Guanylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017665">
        <property type="entry name" value="Guanylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03263">
        <property type="entry name" value="guanyl_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117:SF13">
        <property type="entry name" value="GUANYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117">
        <property type="entry name" value="GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Guanylate kinase-like" evidence="9">
        <location>
            <begin position="2"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="186" mass="20783" checksum="25972A91085DBF1A" modified="2011-11-16" version="1">MSRPIVISGPSGTGKSTLLKKLFAEYPNKFGFSVSNTTRKPRAGEVDGKDYNFSTVEEFQQLIAEDKFIEWAQFSGNYYGTTIKAVKDVAESGKTCLLDIDMQGVKSVKKTDLNARYLFISPPSIDELKQRLQGRGTETEESLTKRIDAASAEMEYAKTGAHDKIIVNDDLEKAYVEFKDFILANE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ30</accession>
    <name>G3AQ30_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycerophosphocholine acyltransferase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_61431</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GPC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375627.1">
        <property type="nucleotide sequence ID" value="XM_007375565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ30"/>
    <dbReference type="FunCoup" id="G3AQ30">
        <property type="interactions" value="190"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ30"/>
    <dbReference type="GeneID" id="18873890"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61431"/>
    <dbReference type="eggNOG" id="KOG2895">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018994_1_2_1"/>
    <dbReference type="InParanoid" id="G3AQ30"/>
    <dbReference type="OMA" id="YIDYYGK"/>
    <dbReference type="OrthoDB" id="406287at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106158">
        <property type="term" value="F:glycero-3-phosphocholine acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036151">
        <property type="term" value="P:phosphatidylcholine acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090640">
        <property type="term" value="P:phosphatidylcholine biosynthesis from sn-glycero-3-phosphocholine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021261">
        <property type="entry name" value="GPCAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31201:SF1">
        <property type="entry name" value="GLYCEROPHOSPHOCHOLINE ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31201">
        <property type="entry name" value="OS01G0585100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10998">
        <property type="entry name" value="DUF2838"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="292"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="388"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="436"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW32351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="457" mass="52899" checksum="5A32F2AC072442D7" modified="2011-11-16" version="1">MSSIDNTRMDNSSSNASIMEVDEDEDAILDAEDVSSSSSTSSQIDTIKHSAPPLGRSDSGSSVSYIDLSKLLFASDFDFGLNIDHMSDSALKKLNNMKRKTQQTLTKYNKKQKKNLELDKLQEIFNQRLAKFDERIHRNLQSSSTEKLFYAVSIFIIAAAGFVIGKYPIYFPHFHTAIFVVLMPIRFYTYFKQSFQYYLADLCYYVNMLLMIFIWVFPNSPRLFVSVFSLSLGTLSFAVITWRNSLVLHSIDKTTSSFIHIMPPVTMFVMVHELPEEYVKQRYPGIAAIDNWNFMSAIIITSIYYTIWQVSYHYFISIRKKDQIENGKVTSFSYLRKKNQKTILGKFVNGLPYLWMQTLAFTLIQFGYQLLTMMFCPIWYKYKHACGAFVTFIFIWAAYNGATYYIDIFGKRLEKEVEKLKREIVSLQQENEKLQFSPLAKPQVPDDDVKPLGEVMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ54</accession>
    <name>G3AQ54_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">GTP-binding protein of the ras family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_61966</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Lipid-anchor</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the small GTPase superfamily. Rheb family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376179.1">
        <property type="nucleotide sequence ID" value="XM_007376117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ54"/>
    <dbReference type="FunCoup" id="G3AQ54">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ54"/>
    <dbReference type="GeneID" id="18874128"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61966"/>
    <dbReference type="eggNOG" id="KOG0395">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_9_8_1"/>
    <dbReference type="InParanoid" id="G3AQ54"/>
    <dbReference type="OMA" id="HQGHGKT"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000273">
        <property type="entry name" value="GTP-binding protein Rheb homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="163" mass="18938" checksum="D50E256FCDFB310B" modified="2011-11-16" version="1">MTVRFVEDHFVESYYPTIENQFSKNIKYNNQDYAIEILDTAGQDEFSIMNEKHLVGIHGYLLVYSVTSRQSFELIEFIRDKILNSIGNEDLPMILVGNKCDLNLQRQVDTKEGQQLAHKFKCKFVETSVKDNVNVNIAFEGLIDDIERIQNPIIKKNDKCIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQQ4</accession>
    <name>G3AQQ4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_140535</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376380.1">
        <property type="nucleotide sequence ID" value="XM_007376318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQQ4"/>
    <dbReference type="FunCoup" id="G3AQQ4">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQQ4"/>
    <dbReference type="GeneID" id="18870298"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140535"/>
    <dbReference type="eggNOG" id="KOG0758">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_16_3_1"/>
    <dbReference type="InParanoid" id="G3AQQ4"/>
    <dbReference type="OMA" id="FGSMAET"/>
    <dbReference type="OrthoDB" id="4153866at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF31">
        <property type="entry name" value="MITOCHONDRIAL ORNITHINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 12" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="24"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="129"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="229"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW31602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="320" mass="35629" checksum="048339697C492283" modified="2011-11-16" version="1">MDISQTTIHNEQQHIASDTQREQIHPIKEITFGAISGMIGKLVEFPFDTIKVRLQSSNSVLPISTFQMISSTYKNEGFFNGFYKGLKAPLYGACLETAILFSSYNLASTFFVNTLNDYDKSSRHHVDSLPFWTKCASGGFAGFAASFILTPVELIKCQLQVVNLVSEGKSTASYSSLIKSTLRNEGVTGLWNGLSSTMTREVIGTSIWFGTYEYINDYYKTAKDPWIKNHDIQLLLSGAMAGVTFNLSMFPVDTVKSNIQTQGIYNKQGINAGFWSIFKSLISKNGGVKNLYKGLGITMVRCIPANALIFYSYEVLKRNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQU6</accession>
    <name>G3AQU6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Succinate-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="8">1.2.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_62254</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 8">
            <text>succinate semialdehyde + NAD(+) + H2O = succinate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13217"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 8">
            <text>succinate semialdehyde + NADP(+) + H2O = succinate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13213"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid degradation; 4-aminobutanoate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.16" evidence="8"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376420.1">
        <property type="nucleotide sequence ID" value="XM_007376358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQU6"/>
    <dbReference type="FunCoup" id="G3AQU6">
        <property type="interactions" value="454"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQU6"/>
    <dbReference type="GeneID" id="18874233"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62254"/>
    <dbReference type="eggNOG" id="KOG2451">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_5_3_1"/>
    <dbReference type="InParanoid" id="G3AQU6"/>
    <dbReference type="OMA" id="IVTGMPT"/>
    <dbReference type="UniPathway" id="UPA00733"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036243">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006540">
        <property type="term" value="P:gamma-aminobutyrate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07103">
        <property type="entry name" value="ALDH_F5_SSADH_GabD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000004">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000005">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050740">
        <property type="entry name" value="Aldehyde_DH_Superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010102">
        <property type="entry name" value="Succ_semiAld_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01780">
        <property type="entry name" value="SSADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353:SF5">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="21"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00052698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="493" mass="54398" checksum="4BF79C9F49288F79" modified="2011-11-16" version="1">MAPKLKNPELLCIKPFINGKWYDSKSSKTFDVFDPATEELIITLPDQTPEEIDEAIQLTHDAFVQYKHTSPYERAKWLRNMHQLMLENLDDLATLITWENGKALCDAIGEVKYAASYFEWFAEEAKRMYGHTIQPSNPSNKVMTLKQPVGAVGLLCPFNFPSAMGARKAAPALAAGCTCVLKPDAQTPLSSLALAYLAKKAGFPDGVFNVVLVSVENTPMVGSKFCESKLIKKISFTGSTPVGKLLMKQSSSTLKKLSMELGGNAPIIVFDDAKLDIAVEQSIASKFRSLGQTCVCANRIYVQEKVYDEFCEKFAAKVRQFKIGNGFEPGVTHACVINQKSIEKVETHLQDALDKGAKLIVPGGRLPELGKNFYAPTVIKDVTQDMRVVQEETFGPLGAIIKFSTKEEVLHWCNDTPFGLASYVFSENLNNVWYMSEYLETGMVSVNTGLFTDAALPFGGVKESGFGREGSLYGVDDYTVIKSITLGNVYHYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQX3</accession>
    <name>G3AQX3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_140703</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375980.1">
        <property type="nucleotide sequence ID" value="XM_007375918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQX3"/>
    <dbReference type="STRING" id="619300.G3AQX3"/>
    <dbReference type="GeneID" id="18870312"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140703"/>
    <dbReference type="eggNOG" id="ENOG502SC5Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143636_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQX3"/>
    <dbReference type="OMA" id="CRKQVVF"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033768">
        <property type="term" value="C:SUMO-targeted ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032183">
        <property type="term" value="F:SUMO binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140082">
        <property type="term" value="F:SUMO-ubiquitin ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049627">
        <property type="entry name" value="SLX8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47094">
        <property type="entry name" value="ELFLESS, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47094:SF1">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="55"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="124"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="124"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="158" mass="17699" checksum="6A2E1B3DA45FCD96" modified="2011-11-16" version="1">MPPLDYIINISSDEEDDIGEEDVEIVEFRKVTNDLIDHPSQKPNHTTKKLSDVQCPICFDDVTNATTTSCGHIFCLECIEQSISSSHARGQVSGNGRGKGLCPLCRKQVSFKDTILIRLKVGERIGKPELPPKEPKKDEIKVTDIKLDEPSPKKRKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR44</accession>
    <name>G3AR44_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Histone acetyltransferase ESA1</fullName>
            <ecNumber evidence="5">2.3.1.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Histone acetyltransferase esa1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17 26">protein 2-hydroxyisobutyryltransferase ESA1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19 27">protein acetyltransferase ESA1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18 28">protein crotonyltransferase ESA1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="ORF">SPAPADRAFT_61797</name>
    </gene>
    <organism evidence="33">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="33">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Catalytic component of the NuA4 histone acetyltransferase (HAT) complex which is involved in epigenetic transcriptional activation of selected genes principally by acetylation of nucleosomal histones H4, H3, H2B, H2A and H2A variant H2A.Z. Acetylates histone H4 to form H4K5ac, H4K8ac, H4K12ac and H4K16ac, histone H3 to form H3K14ac, and histone H2A to form H2AK4ac and H2AK7ac. The NuA4 complex is involved in the DNA damage response and is required for chromosome segregation. The NuA4 complex plays a direct role in repair of DNA double-strand breaks (DSBs) through homologous recombination. Recruitment to promoters depends on H3K4me. Also acetylates non-histone proteins. In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as 2-hydroxyisobutanoyl-CoA (2-hydroxyisobutyryl-CoA) or (2E)-butenoyl-CoA (crotonyl-CoA), and is able to mediate protein 2-hydroxyisobutyrylation and crotonylation, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(2E)-butenoyl-CoA + L-lysyl-[protein] = N(6)-(2E)-butenoyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53908"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:137954"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:53909"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>2-hydroxyisobutanoyl-CoA + L-lysyl-[protein] = N(6)-(2-hydroxyisobutanoyl)-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24180"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:15921"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:131780"/>
            <dbReference type="ChEBI" id="CHEBI:144968"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:24181"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-lysyl-[histone] + acetyl-CoA = N(6)-acetyl-L-lysyl-[histone] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21992"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:21993"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:45949"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the NuA4 histone acetyltransferase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="5"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375997.1">
        <property type="nucleotide sequence ID" value="XM_007375935.1"/>
    </dbReference>
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    <sequence length="385" mass="44315" checksum="25975B8C895E791D" modified="2011-11-16" version="1">MSASISVASEQSSTPGTPMADDEMDLENLNVQGLRRPGEEITREDEIKKLRTGGSMTQNHSEVARVRNLSTIILGEHIIEPWYFSPYPIELTEEDEIYICDFTLSYFGSRKQFERFRQKCSMKHPPGNEIYRDSKVSFWEIDGRKQRTWCRNLCLLSKLFLDHKTLYYDVDPFLFYIMTIKSDQGHHVVGYFSKEKESADGYNVACILTLPCYQKKGYGKLLIQFSYMLSNVEHKVGSPEKPLSDLGLLSYRAYWTDTLIKLLVERNSPSLYRKNNPHPVEDSDSESSRHSTPAAGRQGSSSEITIEDISAITCMTTTDILHTLTTLQILRYYKGQHIIVITDQIMAMYDSLVKKVKDKKKHELNPALLKWTPPMFTASQLRFGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARH7</accession>
    <name>G3ARH7_SPAPN</name>
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            <fullName evidence="9">DUF895 domain membrane protein</fullName>
        </recommendedName>
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        <name evidence="7" type="ORF">SPAPADRAFT_61870</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="MFS"/>
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        <property type="entry name" value="ET TRANSLATION PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294:SF59">
        <property type="entry name" value="UNC93-LIKE PROTEIN C922.05C"/>
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        </location>
    </feature>
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        <location>
            <begin position="10"/>
            <end position="21"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="511" mass="56951" checksum="E4A1CB81286B5E56" modified="2011-11-16" version="1">MDEKSPKIDVNSNSSYDSPSQDLEHQHEHLELKHHTSAEIEADGHWYNKKISFFGTGFRYSSAMTQIVMLAFVVFMTPGMFNALTGIGASIDDKHTADNANVALYSTFATIGFFGGTICNIIGVRASLMLGGTGYALYAGSLLAFNHTRNKGFVIFAGAYLGLCAAVLWAAQGTIIMSYPTESTKGRAIMVFWVIFNLGAVIGSVIPLADNIENKGSAANDGTFIAFIILMCLGSIIAFFMLPMSKVFKSDGTKVMGQKHPYWKDELIGLGKLLLNEPRILLMFPMFFSSNWFYTYQFNNFNYGKFNLRTRSLNSLLYWLAQMVGAVCIGFVLDWQKFRRSIRARIGFVIIFIAGLAIWGGGLKFQLGFTREQAESVPPKFTPLDYTDGGYIGPMFLYIFYGMFDAIFQSYIFWILGALSNNPKKTALYAGFYKGIQSAGAAIAWRLDAIGVSYMALFASSWAMIQVSMLIAAPLIFWYIKDHTSADEDHMDEVVGEEEVTKEFDVQHEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARK0</accession>
    <name>G3ARK0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Stress response regulator protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_67393</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Required for stress adaptation, morphogenesis and virulence.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376099.1">
        <property type="nucleotide sequence ID" value="XM_007376037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARK0"/>
    <dbReference type="STRING" id="619300.G3ARK0"/>
    <dbReference type="GeneID" id="18875158"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67393"/>
    <dbReference type="eggNOG" id="KOG0519">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065405_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARK0"/>
    <dbReference type="OrthoDB" id="303614at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036180">
        <property type="term" value="P:filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900445">
        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050595">
        <property type="entry name" value="Bact_response_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591:SF3">
        <property type="entry name" value="RESPONSE REGULATORY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591">
        <property type="entry name" value="STRESS RESPONSE REGULATOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="114"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00037668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00040436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW31321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="243" mass="27891" checksum="5652FC62CC6F92CF" modified="2011-11-16" version="1">MFATDYYNPFNKIKSTSNNGQLSPNPPILSKTITDYFTIKKNLPIDVSLPENIENGSDFESGDDTNIDPFDKEYCPYTPRTSYLPLTNYDAFSSPIVDPYFPTDVVVPQFTPYKFLVVDDNIINLKILCRQLLKIFPYAEVTKLQDSTKVKPILDTKSFDLVFLDIEMPEVNGIELAQHMRCSERFDQWGVIAVTTLTSVRDLNTYKNCGIDFTFEKPMKADLSEIARIIDEIIRQRKFGLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARK4</accession>
    <name>G3ARK4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_141388</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376103.1">
        <property type="nucleotide sequence ID" value="XM_007376041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARK4"/>
    <dbReference type="STRING" id="619300.G3ARK4"/>
    <dbReference type="GeneID" id="18870372"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141388"/>
    <dbReference type="eggNOG" id="ENOG502SG70">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169147_2_1_1"/>
    <dbReference type="InParanoid" id="G3ARK4"/>
    <dbReference type="OMA" id="QRYQTTH"/>
    <dbReference type="OrthoDB" id="5511599at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039297">
        <property type="entry name" value="COX7a"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02238">
        <property type="entry name" value="COX7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW31325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="60" mass="6851" checksum="DFF5DEFCB497B969" modified="2011-11-16" version="1">MDPQRIINLQRSYQSSKLPLYLRGKYAKPMVYSFIGLFAVTTVHSLYYTGRAVAGLHVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARU1</accession>
    <name>G3ARU1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_141112</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376136.1">
        <property type="nucleotide sequence ID" value="XM_007376074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARU1"/>
    <dbReference type="FunCoup" id="G3ARU1">
        <property type="interactions" value="156"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARU1"/>
    <dbReference type="GeneID" id="18870345"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141112"/>
    <dbReference type="eggNOG" id="KOG0381">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066251_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARU1"/>
    <dbReference type="OMA" id="IFCEMEK"/>
    <dbReference type="OrthoDB" id="10070927at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056513">
        <property type="entry name" value="INO80F"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF13">
        <property type="entry name" value="NON-HISTONE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24245">
        <property type="entry name" value="INO80F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="6">
        <location>
            <begin position="130"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="4">
        <location>
            <begin position="130"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="99"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="201"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="223"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="237"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="254"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="277" mass="31844" checksum="9AAF43EAE6DF2226" modified="2011-11-16" version="1">MKVQAKDTAPESEEQKFKQKCKELKKRVIEIEESNEIATLALSRTQAAIRRLRLEYVILLERLEERATSLPEGIVGFEEMACPPTPSVLDETLTKKNGLAKKQIKRKPASSSAESAPTPKVAKVRDPDLPKRPTNAYLIFCEMEKERIKQENEEKNPGVTNDLSKSMTEAWKLLNEEDRKPYYKLYEDDRDRYQREMAIYNQKKQGTEEVEEPEAKRQKLDTETPEVDSEVPEPVEEVAAPVEEAATPIVKDEPETDPVQEEEDIKEEEETPKIITE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARZ4</accession>
    <name>G3ARZ4_SPAPN</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_62454</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376621.1">
        <property type="nucleotide sequence ID" value="XM_007376559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARZ4"/>
    <dbReference type="FunCoup" id="G3ARZ4">
        <property type="interactions" value="92"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARZ4"/>
    <dbReference type="GeneID" id="18874325"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62454"/>
    <dbReference type="eggNOG" id="ENOG502QQ8J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_3_1_1"/>
    <dbReference type="InParanoid" id="G3ARZ4"/>
    <dbReference type="OMA" id="MRLFCMR"/>
    <dbReference type="OrthoDB" id="10261749at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052765">
        <property type="entry name" value="PGM-Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46192">
        <property type="entry name" value="BROAD-RANGE ACID PHOSPHATASE DET1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="11"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="320" mass="37710" checksum="0C22C84A38C8110D" modified="2011-11-16" version="1">MGKPKYILIVRHGESEGNCDKSVNRYTPNHLVQLTENGHFQALAAGQVLRKFLQDESFVNNCMNEKRNPLSIMFYTSPYLRTRQTCNNIIEGIKDLPGVEYEVREEPRMREQDFGNFQGSAEEMEKMWQERATYGHFFYRIPHGESAADVFDRVASFNESLFRQFRMENFPNVLVLVSHGIWSRVFLMKWFKWSYEEFESLKNIPHCQFLVMKKNKDTQKFNLKTPLLTWDDIEEEDIDEEVSKEIGDALGINQEDLDISMIVRAQRDAIKSTRDKNKQIQEMYAKIKEGSKSKYADKIISRFKYDNQSVVNSEEELHNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS68</accession>
    <name>G3AS68_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62921</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377060.1">
        <property type="nucleotide sequence ID" value="XM_007376998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS68"/>
    <dbReference type="STRING" id="619300.G3AS68"/>
    <dbReference type="GeneID" id="18874527"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62921"/>
    <dbReference type="eggNOG" id="KOG2975">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027018_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS68"/>
    <dbReference type="OMA" id="QDVIMIE"/>
    <dbReference type="OrthoDB" id="25498at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024969">
        <property type="entry name" value="EIF3F/CSN6-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540:SF6">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13012">
        <property type="entry name" value="MitMem_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="1">
        <location>
            <begin position="29"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="321" mass="35500" checksum="FBF7D74C35DE38F9" modified="2011-11-16" version="1">MSSDNSFMHLVRPGVSAPAAASTAHNAQVKIHTPAMLSILEIVSKQIISSNKRIIGTLLGSRSEDGNIFEIMDVFMVPCNETGDSIAIEDQTHKTLYQLYKKSHPKQSVLGWFGSSNTIDSTTSLIHDFYSKGVDRAYPFPAIHLNVEYLNNDNQIVAPKVTTYIGAAVGKPATTAQIGWNTNVATNSYIFTPIPNQLINGTTTEKIAFNNLQQGVITNTTNLHQDLSYLSQQLEKVDSNLSELINYIDSGKSDNVDLLRLLSNTLLNRPQLLSNIEELQKLFRDHNQDVIMVEYLTKAVKDQIELSARLTAASGENKYEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS71</accession>
    <name>G3AS71_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Alpha-1,3-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_62923</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377063.1">
        <property type="nucleotide sequence ID" value="XM_007377001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS71"/>
    <dbReference type="FunCoup" id="G3AS71">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="GeneID" id="18874528"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62923"/>
    <dbReference type="eggNOG" id="ENOG502RZ48">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015387_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS71"/>
    <dbReference type="OMA" id="VYGACYL"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000033">
        <property type="term" value="F:alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392:SF1">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="759" mass="89049" checksum="3F3C3A8B1DEA158F" modified="2011-11-16" version="1">MPKWKSNSKFNLAVYGGGFLWVLCITYFIHYNYLRSEHSLVPISNPENNADYHGNVFAEQEEEQESDKSRPELTHDRTFLMLKQLVSKKAKGSDMYGTIYDKIFKHHDLSSVIANLDFNERCNLYFKNLFLQNHDWKLDPHIDLDLHWDGDEFKGYFQDHETEFLEEFNKRLYLPQDKEEYNKHFEEFVRNKFKAYKQPYLDREMVDSLTTFRIFNQCYLDGQTKSQKFITEQKQFLNRVGKAAAPFQEIQSEKLTDFSTYESFEHRVYPWLSFEYPVYEHWSGATFYKPPNLKKVDFRGKTTKRSFASKPKAGPKSQFLRELKSLSNGRGIILTMPESHVDSTVNLIHLLRALNNKLPIQIVSTDDISLKAKRKLVAAARDDYVTLPKSFEKVAEHFPKDYLTKNGGLPKQELWFVNAHNTINDLYRDKFVSYANKFIAILFNSFAEFMLVDDDTAMMKSPEWFFNLKGYTETGAYFYKDRTSPERRAISDGEMFHRMSPSLVDTIMFDIPVMTQHTLDREFFKGLFHYQEAGMVLVDKIKHFNSLLMVTQISFFGPVHGKMWGEKEMFWLGFAINGDESYEFNENFAAAIGELTDFRDRHRSDGSHHRSRELCSNHPGQIYDGDGHTLLWINSGLRFCHQAEQVDFEDEFKLGSRLKFLHDIEAFKAFYFAPLRITHAVVPPLTDDLQPRGNFQDEPGYGWMMEGSYCRRYLWCAYSSVGGEGHNGRDNYVEGTIVDFTQKEQDLFKYYGDIWVGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS76</accession>
    <name>G3AS76_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription factor BYE1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_156645</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Negative regulator of transcription elongation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BYE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377068.1">
        <property type="nucleotide sequence ID" value="XM_007377006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS76"/>
    <dbReference type="STRING" id="619300.G3AS76"/>
    <dbReference type="GeneID" id="18871054"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_156645"/>
    <dbReference type="eggNOG" id="KOG1844">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024990_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS76"/>
    <dbReference type="OMA" id="RTHKGDI"/>
    <dbReference type="OrthoDB" id="79252at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061188">
        <property type="term" value="P:negative regulation of rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061186">
        <property type="term" value="P:negative regulation of silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.30">
        <property type="entry name" value="Transcription elongation factor S-II, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053051">
        <property type="entry name" value="HDAC_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012921">
        <property type="entry name" value="SPOC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036575">
        <property type="entry name" value="TFIIS_cen_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47793">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT CTI6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47793:SF1">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT CTI6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20826">
        <property type="entry name" value="PHD_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07744">
        <property type="entry name" value="SPOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07500">
        <property type="entry name" value="TFIIS_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46942">
        <property type="entry name" value="Elongation factor TFIIS domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="9">
        <location>
            <begin position="59"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="123"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="312"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="41"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="330"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="349"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="362"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="380"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="614" mass="70696" checksum="CDBBBDA4B11BAC95" modified="2011-11-16" version="1">MTAQRRSSRSTKGKHSKRFTDEIIGDDLDLLDKHRVKKQKIQEGVHEDDKEYKQEDEGVVDCRPCGTNQDNYDEENDQGGTFIQCDKCNTWQHAKCMGFTRNKRIPENYTCDQCSPELYDKEKLKRRSAKSQTPEKKTSTEEEEPKLDTKPVVTLDSLKDDTRLSTAKAFFNFFKRSFPPEDQSSTEAKELKATEWALEIEDIIFREFQGKLYTSEGRRILFLLKKYFMKDILSGTITFEDIVKKTPKEINQNIEKIEAQNKENIKNIILHENDTNEIIRRTHKGDIVKENENDNDRVDWIDASITTKKVDHRIFSSEEEGTPDVRKPSITASSIEQSTYNNLNPRIEDSDDDDDDDEEEEEKKQEKVEENTVKTEATSEKQNLSPETKSSESSDLEQVEHPDEDALFPILTGGKPIKPVEIVNPKVWEGTIEFPDFTQFEAIGEFHTCTNYSINHAKGQAICHDILDQKIYHVQGRLDRKVADGYLNKITSSRDLIFVEIKHAPLQEDQFNKLYQYLLLEDKVGVLSGKPWFVKDAYIMPIDFRDDNMPSYVKSHKRDMRIGLFAAFVVKRDYRPSTGALAADNVTSTSSYNEGLSFEAIMSQLGGSNNSTYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASG9</accession>
    <name>G3ASG9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_141865</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377120.1">
        <property type="nucleotide sequence ID" value="XM_007377058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASG9"/>
    <dbReference type="STRING" id="619300.G3ASG9"/>
    <dbReference type="GeneID" id="18870404"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141865"/>
    <dbReference type="eggNOG" id="ENOG502QR55">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070142_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASG9"/>
    <dbReference type="OMA" id="ICRANDK"/>
    <dbReference type="OrthoDB" id="8249012at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21521">
        <property type="entry name" value="AMUN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21521:SF0">
        <property type="entry name" value="AMUN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="341" mass="39043" checksum="94274F10FECFE7F0" modified="2011-11-16" version="1">MFDLKQIRRASNQLYSDIIAEFSETRGKYDNDSKTFQELNQWRLQELPETLAQRLEDTKSIWLTKDELVLLMDWKLANGKFRATLPKLIRSNDESTVEAVTKQGFQIWLTFRRTTGTNDLWDDFAYKGMIKSSFKKLCELRGVGPATASLILSLIHKIDKKWAPPYFSDESFLYYVNPEDKIKYTVKEYLDLFLPAFLVLHVQDSDVSMDELERGAWALKMYELHKDDTLANVKPAEEDAPEAKAIEENGTKKRSRGSIKKELESEETEITEQEAKPKRARATKAAYKAESLSKAPKSTKAASTRNAKKAAKAEGIAKATKVKSKTSIKSEEPKPKRTRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASH5</accession>
    <name>G3ASH5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_63020</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377126.1">
        <property type="nucleotide sequence ID" value="XM_007377064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASH5"/>
    <dbReference type="GeneID" id="18874556"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63020"/>
    <dbReference type="HOGENOM" id="CLU_1195521_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASH5"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="232" mass="26040" checksum="D6F2CBF27E09EB3D" modified="2011-11-16" version="1">MLIRTFILVSTFSIHVFASVFTVKLYSRSVNQEYNGQGVSIEGSSLVLGDAKEFQYDNESKFLFIVEPGKKVYFSLSDSKLELSSKDKVEVVLVEGHNRLVEFKHSDALYAIKSESESIISLGYSDDGPIDGGIPIQLVYQNVKNVDKTATEQSITETKSTQKIDTTQQEPTSTQMTNYPDKSDKMLSGSTKSRSFFGSVGKTLVIIYCDIVFVYQLSIITFFAVFFFFCLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASL2</accession>
    <name>G3ASL2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methionine permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_56690</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376731.1">
        <property type="nucleotide sequence ID" value="XM_007376669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASL2"/>
    <dbReference type="STRING" id="619300.G3ASL2"/>
    <dbReference type="GeneID" id="18872059"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56690"/>
    <dbReference type="eggNOG" id="KOG1287">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013661_4_1_1"/>
    <dbReference type="InParanoid" id="G3ASL2"/>
    <dbReference type="OMA" id="YPGTWIN"/>
    <dbReference type="OrthoDB" id="5982228at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF382">
        <property type="entry name" value="LOW-AFFINITY METHIONINE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="395"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="430"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="472" mass="52395" checksum="B3F09A802E8726A8" modified="2011-11-16" version="1">MIPASDIGRRASQEKLGTLSCISLIVNKIIGTGIFSNPSPIFQYTHGNVGIFLLLFFLGGVIIFCGLLIYLEFALNLPFKNGGELNYLMRSFKRLPKGLTGCIYSFSIVILGFSSGNSYAFGKYVLFAITGKQEMNDTIVKAIGVGCISFCIWLHIKFPNQGTTLFNFLGIFKILILVLIIIIGGLASLGLLNIETTDNFNGDIWKFHNNVKPNSYSIAVALLEVIYSFKGWENVNYVLNEVDNPYHILTVAAPLAVALTTGLYFLVIISYLIIIPKEEILSSGVLIAGIFFNKVFGESITARLLPVFISLSNLGNVLVVSYAHSVVNQELAYNNYMPFSKYFQNLSHSLLLHGAITIIILVAPPSTEIYEFIVNLYIYPGTWINVAISEKDKKISAPMVCVVIFLVSNIFLALFPFVPPPNKDSLAIPYWMFPVLGTSVLVLGGVFYYVRPLWSKHEDKEITYGDDYNPLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASL8</accession>
    <name>G3ASL8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Luciferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_72649</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the NtaA/SnaA/DszA monooxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376737.1">
        <property type="nucleotide sequence ID" value="XM_007376675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASL8"/>
    <dbReference type="GeneID" id="18875447"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72649"/>
    <dbReference type="eggNOG" id="ENOG502QSR6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022256_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASL8"/>
    <dbReference type="OMA" id="PGAKYFW"/>
    <dbReference type="OrthoDB" id="5561043at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051260">
        <property type="entry name" value="Diverse_substr_monoxygenases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016215">
        <property type="entry name" value="NTA_MOA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03860">
        <property type="entry name" value="FMN_nitrolo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011">
        <property type="entry name" value="ALKANESULFONATE MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011:SF16">
        <property type="entry name" value="C2H2 FINGER DOMAIN TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000337">
        <property type="entry name" value="NTA_MOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Luciferase-like" evidence="6">
        <location>
            <begin position="42"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="501" mass="56237" checksum="74747064B71926DA" modified="2011-11-16" version="1">MTSETKRQKKQRKPLIINAFDMGCPGLQTPGLWKHPDDKSRDYNTIEYWTNLAKLLEKGKFNALFIADVLGPYDVYNGPANLTAAAKSGAQWPINEPTAVISAMAAVTKNLSFAVTASTISEAPYHWVRRLATLDHLTKGRIGWNIVSGYLDSAARNLLNGEPLPEHDARYDRAQEYLEVVYQLFLSSWADDAVVLDKKRGIFSDPERIREINYKGEFFDVPGPNITEPTPQRLPVILQAGSSSKGMKYAAQNAEAVFITGMTPEGLGYKIKQLKEEVKKAGRDPATVKTVQLVTVIVAPTHEEAVAKFKEYKELSDIEGAQALFGGWTGIDLAKYGWDEELDKIEGNAIRSMVETWTKPFPGDPENLKKTRGYIADRICIGGSGVLFLGTPQEVADEIEEWVDVSGVDGFNFTYAITPGSFEDIVELLMPELRRRGLAWEDYPKEGLTFRENLYGLDREEPTYLPKDHPAYNLRWKAGESKEEFEAKLKKTLEEHFKKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASW8</accession>
    <name>G3ASW8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DASH complex subunit ASK1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Outer kinetochore protein ASK1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_67993</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex ASK1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376783.1">
        <property type="nucleotide sequence ID" value="XM_007376721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASW8"/>
    <dbReference type="STRING" id="619300.G3ASW8"/>
    <dbReference type="GeneID" id="18875193"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67993"/>
    <dbReference type="eggNOG" id="ENOG502RZQN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037658_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASW8"/>
    <dbReference type="OMA" id="RYGVECE"/>
    <dbReference type="OrthoDB" id="5573898at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013964">
        <property type="entry name" value="DASH_Ask1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28200">
        <property type="entry name" value="DASH COMPLEX SUBUNIT ASK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28200:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT ASK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08655">
        <property type="entry name" value="DASH_Ask1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="115"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="225"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="269"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="280"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="306"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW30750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="524" mass="58351" checksum="78009D8D6FC84861" modified="2011-11-16" version="1">MSRRVSIIPKRKSFIEKTGDEEEEEELKAELNRLDWETSALLREIDRKLVIAIDNVNDKLMPAIARYGVECEEISKFGEMWKLFFARAAKVELQAYEEPIHSGSEGMLFGNDDRQEQEEYTEDTQRQQQQQQQPIEEDGSKPFNKLVNKGILVQDEESITWSTEQAPNKPYVSSTPQRIPKASTTTVNGAAHTLAPVNINLGPPSNDVQITRTYRRSLDAYHRISVSPEKVRDGKGLRTPRRRTIIDDILDSSPPLPPPPILLSDRYEQNTPGSRLTPGSQQLSSSVQRNQQQQQQQLSSAQRIQGSGSKRLSNTPILDQHLSGSVPRTGSVRRSGSVPRGIIGSSSAAAAAAAVTTAAEMSTPLANRSRISENEIVPVQPSGDRVEQEEHEIPLLELETTEIPNQGSNKRRKLDNPADNAEENVFLDNTGNNSTLFYSPNNKETNGDNTNLSLTQQYEENMARVSRNAEVPPATGIIPPQSNILDSDPELARLRSQLGPLAEKYLEFRKSNERILGTPSIAFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT14</accession>
    <name>G3AT14_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Indoleamine 2,3-dioxygenase</fullName>
            <ecNumber evidence="5">1.13.11.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">BNA2</name>
        <name evidence="7" type="ORF">SPAPADRAFT_63090</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Produces N-formyl-kynurenine through the oxidation of tryptophan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-tryptophan + O2 = N-formyl-L-kynurenine</text>
            <dbReference type="Rhea" id="RHEA:24536"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58629"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the indoleamine 2,3-dioxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.52" evidence="5"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377207.1">
        <property type="nucleotide sequence ID" value="XM_007377145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT14"/>
    <dbReference type="FunCoup" id="G3AT14">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AT14"/>
    <dbReference type="GeneID" id="18874590"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63090"/>
    <dbReference type="eggNOG" id="ENOG502QV6W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010089_0_0_1"/>
    <dbReference type="InParanoid" id="G3AT14"/>
    <dbReference type="OMA" id="SNKIMEP"/>
    <dbReference type="OrthoDB" id="540174at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033754">
        <property type="term" value="F:indoleamine 2,3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004833">
        <property type="term" value="F:L-tryptophan 2,3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.480:FF:000004">
        <property type="entry name" value="Indoleamine 2,3-dioxygenase subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000898">
        <property type="entry name" value="Indolamine_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037217">
        <property type="entry name" value="Trp/Indoleamine_2_3_dOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28657">
        <property type="entry name" value="INDOLEAMINE 2,3-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28657:SF5">
        <property type="entry name" value="INDOLEAMINE 2,3-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01231">
        <property type="entry name" value="IDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140959">
        <property type="entry name" value="Indolic compounds 2,3-dioxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00876">
        <property type="entry name" value="IDO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="4 5" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="385"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="binding site" description="proximal binding residue" evidence="4">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600898-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="454" mass="51305" checksum="E07A630BCABE6166" modified="2011-11-16" version="1">MTATIDIPRMEDYNVFPNSGFLPEKLPLERLPQQYYKPWEDIATNLPSLILTKRVRSVIDKKLPLLETSQLTSEEEYRRAYQVLGFLAHAYIWGTEEPTNKLPPQIAQPWVEVSDHLRLPPIATYAGLCLWNFKQIIPTEEGEDIWDLDNLTTINTFTGSIDESWFYLVSVYFEHKGALAITTGLQAIKCAYNNDIPGLVAQLQQLAEAIDALGSVLLRMEEMCDPHVFYFRIRPYLAGWKNMADVGLDKDGVLYGNESKPRAYSGGSNAQSSLIQFLDILLGVKHYSTGERPLPFRKDVPKTGPENGFMNEMRTYMPGPHQDFLKKIHEITNIHDFVLQNVDTHPELVLSYDGCLAMLKSFRDKHIQIVTRYVVLQAKKTNNMGSSTTLRSGLARKKKNGGEEKGTGGTSLLPFLKQCRDETGDPAAGNWGKRILSDGVMRLKYSKPNGINED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT64</accession>
    <name>G3AT64_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_52025</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376860.1">
        <property type="nucleotide sequence ID" value="XM_007376798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT64"/>
    <dbReference type="FunCoup" id="G3AT64">
        <property type="interactions" value="352"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AT64"/>
    <dbReference type="GeneID" id="18871620"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52025"/>
    <dbReference type="eggNOG" id="KOG1274">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004219_2_0_1"/>
    <dbReference type="InParanoid" id="G3AT64"/>
    <dbReference type="OMA" id="RYAHTNG"/>
    <dbReference type="OrthoDB" id="427368at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022100">
        <property type="entry name" value="WDHD1/CFT4_beta-prop_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048591">
        <property type="entry name" value="WDHD1/CFT4_hel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19932">
        <property type="entry name" value="WD REPEAT AND HMG-BOX DNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19932:SF10">
        <property type="entry name" value="WD REPEAT AND HMG-BOX DNA-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20946">
        <property type="entry name" value="Ctf4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12341">
        <property type="entry name" value="Mcl1_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDHD1/CFT4 second beta-propeller" evidence="6">
        <location>
            <begin position="415"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="WDHD1/CFT4 helical bundle" evidence="7">
        <location>
            <begin position="751"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="324"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="381"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="725"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="324"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="728"/>
            <end position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="860" mass="97269" checksum="9B943AFF64586403" modified="2011-11-16" version="1">MSHKKITAFPDGNSYVHYNPTSDRLLIGNSEGLIKLFNIAEPELEPVSIDIIDNLTSLAHDSDSNRAVVTSTSGQLELIDLKSGESNGIIHRSDLPLRDAVFVNSGKRVVCGGDDDKLVVVDVQDKSTQSITLPDQMVNLAYNFSGEIISVCLSNGNVQLYSVVNEQPNLIHSLNNVLNAKINTSLDQIDFNQENHDELVCSKTHWSSNGRYLLVPTIENEVHVYQRDDWKIIKKFDNDNAKIVDFALSANGKHLVILSHDGFKIFDFDSGNVIHEDTVDFDGYYPLNTAWRGNHLFIGTTNGEVLSLKNIVSESASNLFVDEAEESDIEDDEGEKDDGNDTDVLLRDSDDENEEPALARKREYKLHEEDELIIDEDDEELNGLSNGHSESYKRSKLNGHSTSNGHAHVPTFEIKPYSPGSTPFNHGSVKKRYLTMNNLGYVWIVKTIELESQQSITVSFFDRSVHREYNFIDYSGFDLASLNARGILLARSADSKKEGGLVYYRDHDDSYSWERKIPLVVNEYITSVCISNSNSENDLIVVGTNMGYLRVFDQFGLCVNVIKTTPVVSLISSSRATLFMINQVANVFTYSIIELADFKYYQQNVMLPLKHSDKLLIKGVFFNEYDDPILVGGEDDTVLVLHQWRQEQNSKWIPLLNCHDAIVGHNRNKKNWKCWPLGLIGEKLNCLILKGGDSEYPGFPLPLPVEIDIEMPIVVKERAKEKKKSDLDDLGLGEGDDDDEEDEEETRVEEDAEEVFVRSTTFGKLLSETLNDENTEDTDRSDIMERLNECANLFDKSLLKLFGQACSQSQLNKAWSIVKMIKKDKAQYAAFRIAERMQFMNLANKIRELREMSLEEEEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATD9</accession>
    <name>G3ATD9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62815</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376935.1">
        <property type="nucleotide sequence ID" value="XM_007376873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATD9"/>
    <dbReference type="STRING" id="619300.G3ATD9"/>
    <dbReference type="GeneID" id="18874477"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62815"/>
    <dbReference type="eggNOG" id="ENOG502QRM1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1066232_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATD9"/>
    <dbReference type="OrthoDB" id="416217at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053157">
        <property type="entry name" value="Sterol_Uptake_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784:SF5">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="261" mass="29054" checksum="D876C7C7E92A326E" modified="2011-11-16" version="1">MDSLANASFPSSTSPISLPASAWIFHVKGAATILTAVWPLTEASRFYKFISVDLGDLGDIINQRVNLGKKARNGSGDSEQQQQRFYTDLECHDSDIADLYPVEIDSPYLMTLAYLNKLHKERYKSDFILRIFAFPALLDKVFLTLLMSGDVKAMRIMRSYYKLLRAFTTEMKDKVWFLEGVSQVLPVNVEEYAGGAGGINMMLDFLGGGPAMVEDELEDSIVEFDAAGNLNQKLPDMNGMPSEITSGMEVMQGDNGFMNIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATG0</accession>
    <name>G3ATG0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sorting nexin-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_62837</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Required for retention of late Golgi membrane proteins. Component of the retrieval machinery that functions by direct interaction with the cytosolic tails of certain TGN membrane proteins during the sorting/budding process at the prevacuolar compartment. Binds phosphatidylinositol 3-phosphate (PtdIns(P3)).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Prevacuolar compartment membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376956.1">
        <property type="nucleotide sequence ID" value="XM_007376894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATG0"/>
    <dbReference type="FunCoup" id="G3ATG0">
        <property type="interactions" value="483"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATG0"/>
    <dbReference type="GeneID" id="18874487"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62837"/>
    <dbReference type="eggNOG" id="KOG2527">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057172_2_1_1"/>
    <dbReference type="InParanoid" id="G3ATG0"/>
    <dbReference type="OMA" id="NMYTDYE"/>
    <dbReference type="OrthoDB" id="5227681at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030904">
        <property type="term" value="C:retromer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034499">
        <property type="term" value="P:late endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051074">
        <property type="entry name" value="Sorting_Nexin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45963">
        <property type="entry name" value="RE52028P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45963:SF2">
        <property type="entry name" value="RE52028P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="14">
        <location>
            <begin position="33"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW30923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="158" mass="18464" checksum="D8094F663240A148" modified="2011-11-16" version="1">MSRPFQPISDVINTSPPKRQTQSFNEIYGEPEDFLEVEVRNPVTHGYGSNLFTDYEIVCRTNIPAFKKRNSRVRRRYSDFVVFRKILEQETTRVIIPPLPGKILLNSNKFDELNIEKRRQGLEKFLTTVAGHPLLQTGSKTLIEFIQNEKWDAKFANY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATG6</accession>
    <name>G3ATG6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Beta transducin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_52119</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat WDR3/UTP12 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376962.1">
        <property type="nucleotide sequence ID" value="XM_007376900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATG6"/>
    <dbReference type="FunCoup" id="G3ATG6">
        <property type="interactions" value="1229"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATG6"/>
    <dbReference type="GeneID" id="18871630"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52119"/>
    <dbReference type="eggNOG" id="KOG0306">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005318_0_1_1"/>
    <dbReference type="InParanoid" id="G3ATG6"/>
    <dbReference type="OMA" id="MNIPLTC"/>
    <dbReference type="OrthoDB" id="407922at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001139">
        <property type="entry name" value="DIP2p Nucleolar protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000157">
        <property type="entry name" value="WD repeat domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000178">
        <property type="entry name" value="WD repeat domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051570">
        <property type="entry name" value="TBC1_cilium_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19853">
        <property type="entry name" value="WD REPEAT CONTAINING PROTEIN 3 WDR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19853:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25172">
        <property type="entry name" value="Beta-prop_WDR3_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="76"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="118"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="160"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="406"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="497"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="596"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="638"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="680"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="8">
        <location>
            <begin position="819"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="739"/>
            <end position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW30929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="960" mass="107762" checksum="50A4ECE7937953C4" modified="2011-11-16" version="1">MVKSYDRYDQEKSFGVITSQSNIVWLPPSDVQSSSTSAGRALTSALEEILIWDIKTGEILSRLRDGLTPGASNAPTSSPPSVVTTLAFHEMTNIVAAGYGDGSVKVWDVSSASVVISFTGHKSAITQLKFDRSGTRLVSGASDSTIILWDLVGETGLFKLKGHKGPISGIEFLSEVYDNDNVDDLEDYLLSSSRDGLIKLWELKSQQCIETHLAHSNECWSMAVDPKRRDMVITSGNKDQVKVWHLDLGEEDGSKITERGSFTKTSKNRSNEIVFKRAKVGSSYLDLFAVQNSDRTVELFRIRSEDEIKRGITKRTKRLKEKGLDDDEILQSIQESEISMLITPFTTLRTMAKIKSISFTNTSKQLNLLISLTNNSIEYHNVPIPENPKKATPGELYSVKKHSLDMAGHRFDIRAMDVSDDSKLLATASNGELKIWNLRTYNVIRTFTLEGGYALCCKFLPGGSLIVVGFKNGDLELYDLATSSLVDRVERAHSLANDDESAAIWSLDVSPDGKTLVTGGNDKCVKFWNFKVEQEVVPGTDAVISQLRFKHTQTLDLNEDVLCVKISPDGKYLAVSLLNNNVQVVFFDSMKLFLTLYGHKLPVLSIDISSDSKLIITSSADKNVKIWGLDFGDCHKSIFAHQDSVMNVKFIGDSHNFFSSGKDGLIKYWDGDKFECIQKLAAHQSEVWCMAISSDGLVMVSTSHDHSIRVWSATDDQVFLEEEREKEMDELYENTLLNQVAEEERPRDDDEEEVDEATKVSRQTMETLKAGEKLMEALDIGIEDIEAVKEYESLVKQGKSAVKPTPNAILLAFNMTGWEYVLSVLIKIRPSQIDDALLVLPFSYSLKLLQLIEIWTNKQNINTNIVNLSLICKVLFFIISSNANEFINQKDPAIKNQLIKVKQQLRDQLSGTCRQLGVNTEALKFVKQQWKLTHETEFIDERQQREYEDKKAIKRTYTTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU19</accession>
    <name>G3AU19_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_63244</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377366.1">
        <property type="nucleotide sequence ID" value="XM_007377304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU19"/>
    <dbReference type="STRING" id="619300.G3AU19"/>
    <dbReference type="GeneID" id="18874657"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63244"/>
    <dbReference type="eggNOG" id="KOG0287">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_924914_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU19"/>
    <dbReference type="OrthoDB" id="9049620at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097505">
        <property type="term" value="C:Rad6-Rad18 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006301">
        <property type="term" value="P:DNA damage tolerance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039577">
        <property type="entry name" value="Rad18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14134">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RAD18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14134:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RAD18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAP" evidence="2">
        <location>
            <begin position="87"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="301" mass="33621" checksum="24C0EE239D0305BA" modified="2011-11-16" version="1">MQMSAEILQTSHLDDCLSGKVTKKYTAKPKKKQPRNSISSFFKPRSLVSSSSSSSSTPPEKLGNEAYYFQETSRHNSEVKRLPKLDFSSLITPKLKEKLAAINLPTHGNRNQLELRYNQYFVLYNSNLDSNRPVSEKVLRQKLNQWELSHREFNSYNGDLFSNHQRGISNRSITDKNFSVSEWMQEYKQDFKELVKIARASAKKTGTTDTADKAVEVEVEVAAAAAAAVEIVEKSDSYNSDITVDEMNDNLTELPQETTNDSADNSGTAAVSAKITGEEEIKIADLDFASSPLFSDAVGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU44</accession>
    <name>G3AU44_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_143563</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377391.1">
        <property type="nucleotide sequence ID" value="XM_007377329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU44"/>
    <dbReference type="FunCoup" id="G3AU44">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="GeneID" id="18870554"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143563"/>
    <dbReference type="eggNOG" id="ENOG502R1XI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_334934_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU44"/>
    <dbReference type="OMA" id="SEGMWFE"/>
    <dbReference type="OrthoDB" id="2155291at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="2">
        <location>
            <begin position="545"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="811"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="848"/>
            <end position="872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="884" mass="100864" checksum="1DC701371975767E" modified="2011-11-16" version="1">MASPTHFANSYWSSDYKTAIDQLQNESTTSLAQLHELRKLVFSYINYFTGNSEYLDKSSNDLLPRSSPFSVNKASPSRRRVVSASVVASESVTPQPDSVEEITMSMAYNNYINHMKFDSQLLIQMASIIDRDVLEEITQFLKYEEPKLKGEVSRLQEAYIEYLTSFNKIEKLKSRHQEQLRLKEFNDSSIKISIEDTSVNDSIASDDSYTTEADSSIIDSTKAADNGFDEFEFPLKLGSAPISNRDELRDLMNQLISKVPTIKRKIPIPGYKPDIFSSDSLCGVVSKMRLRGLTPSRMNLERFGQSLLDMGLIVTSQFLASKKFKSEGMWFEWSDLAYFVREYDDEATDVETPTLHATALAASPSSTKFQSEVAETTKRLNAMFNSVKSSIMKTNHDEMLIDIQQKYEQEYLSLEELKFRLENGTLQITQYLQEFEKNKIEIVYKSLSRLSEILNRFSHKQAETLEHFSNDFINRINRSANYQHDLQLVLKKYSTGIYFPSNTSPEALVKKSPGQSGAHYQNLKYQFNLYKDIPLQLQVGQLYPASLLSITSIPYILYQAIKIIETDHESCRKYWLSPIDYQSVWEIKQDIIMLISNFTPDLNAEISNERLIQGEFLNKVVEYLKGKSIENVVCFLKSWLLEISDSCIPFVVYDSIAHIYSKENTVDELLKQLSTIPRSNLATLVFVLEHISRVFDMSVISQYEVSDEIEEGEDEANLEEVAAKLNSMDEIGAVPFVHLIMRPSVVKHSSGFKPPIETYVKFLQDLLKLKVRSHLLKKLIDNEQTIKRKRLETEKNGLSLKRVSLVVPEAEPTIPTTPKKETTNMSLSIKSPRPVSGEFSLRPFRTRATPQPSPRGSPTRSPVISRESSPVRSGSVLLPTVKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUU4</accession>
    <name>G3AUU4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_52875</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FKBP-type PPIase family. FKBP1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377801.1">
        <property type="nucleotide sequence ID" value="XM_007377739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUU4"/>
    <dbReference type="FunCoup" id="G3AUU4">
        <property type="interactions" value="388"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUU4"/>
    <dbReference type="GeneID" id="18871729"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52875"/>
    <dbReference type="eggNOG" id="KOG0544">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013615_12_0_1"/>
    <dbReference type="InParanoid" id="G3AUU4"/>
    <dbReference type="OMA" id="EQFDASW"/>
    <dbReference type="OrthoDB" id="1902587at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005527">
        <property type="term" value="F:macrolide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901711">
        <property type="term" value="P:negative regulation of homoserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903332">
        <property type="term" value="P:regulation of protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000061">
        <property type="entry name" value="FK506-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050689">
        <property type="entry name" value="FKBP-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10516:SF443">
        <property type="entry name" value="FK506-BINDING PROTEIN 59-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10516">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="6">
        <location>
            <begin position="23"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="121" mass="13047" checksum="5F0AC4F3A79490C5" modified="2011-11-16" version="1">MSEQLPQIEIIQEGDNKTLAKPGDVVTIHYVGTLTNGKKFDSSRDRGKPFTCTIGVGQVIAGWDIALTNNFGKGLATPIYKGSKAVLTIPSELAYKDRGFPGLIPPNSTLIFEVELLDINA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUV5</accession>
    <name>G3AUV5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sec1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_144567</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377812.1">
        <property type="nucleotide sequence ID" value="XM_007377750.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUV5"/>
    <dbReference type="FunCoup" id="G3AUV5">
        <property type="interactions" value="828"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUV5"/>
    <dbReference type="GeneID" id="18870645"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144567"/>
    <dbReference type="eggNOG" id="KOG1300">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009210_1_0_1"/>
    <dbReference type="InParanoid" id="G3AUV5"/>
    <dbReference type="OMA" id="PFTRPHT"/>
    <dbReference type="OrthoDB" id="2228at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031340">
        <property type="term" value="P:positive regulation of vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="549"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="678"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="705"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="555"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="706"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="742"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="759" mass="86577" checksum="3806765A0C237D03" modified="2011-11-16" version="1">MSSNNKVRDPTSLLNIQHDYLLSQIKQAQVPGQLYNLIIDSTTESILYRVITKEQLLRIVTSIEKIDERRKNAGKFINAIYFVDIDIYNINCMMADAETNRFKSGVGLFLPLSSHSSETGHYFNSRFLQNPKVTAYFNQGASINYINANMYPMESRVFLTDNRTPNSMPIYFNENCSEFVKLQIEKAAKSLVSLMVLTGEYPLVRYYSPQGTSHQAQPLCELLACEFQNQIDEFARLNQDYPPASVADKPRSILLICDRTLDLFAPLLHEFTYQAMAMDIVPNLERTGVYKYQVETESGETKEAEATLDDEKDEDWVNLRHTHIIEASELIIARINDLIKNNPLMVDRSKASTSSDLMYIVAHLKGFDEERKNLTLHKTLIDECLDINSSRKLAEFAADFEQTCCADGVTFEGERNKTLHDDLIVLLAREDLHINDKIRLVLMYAFYRGGLSESDFKKLAKFIGVNDREIVGLISRCFNNLHKLGFPIVKASAKDKPVVKTTFHTINNEGTYNTSRFGPAIKQVLTNASRYHLDEEWFPYFRDKPLQDDLPASARPTSNSRVNQLNNGTGSLRNPRIKASWASSSSLRHTGSTTSSIHNPLNQKIFCYVAGGITYNEMRSIYELSHSLNKEFYIGSESILKPRDFLIGLQCIDKAKSIKDLDLSIAREMNKPQECPSHLYETIASPPPPRTVSTPQNQVQEKMNRFEQQVQQQQQSFESQHASSTSHYKKRLSLSPNLSGDSKFKEKEKKTSRLKKLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUX6</accession>
    <name>G3AUX6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histidine kinase/HSP90-like ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_63686</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377833.1">
        <property type="nucleotide sequence ID" value="XM_007377771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUX6"/>
    <dbReference type="FunCoup" id="G3AUX6">
        <property type="interactions" value="1559"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUX6"/>
    <dbReference type="GeneID" id="18874868"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63686"/>
    <dbReference type="eggNOG" id="KOG0019">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006684_1_3_1"/>
    <dbReference type="InParanoid" id="G3AUX6"/>
    <dbReference type="OMA" id="MRRMKEM"/>
    <dbReference type="OrthoDB" id="28737at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140453">
        <property type="term" value="C:protein aggregate center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16927">
        <property type="entry name" value="HATPase_Hsp90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.790:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.80:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11260:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000001">
        <property type="entry name" value="Heat shock protein HSP 90-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="entry name" value="Heat shock protein 90, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00505">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019805">
        <property type="entry name" value="Heat_shock_protein_90_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037196">
        <property type="entry name" value="HSP90_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003555">
        <property type="entry name" value="PRK05218.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="HSP90 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00298">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="9">
        <location>
            <begin position="29"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="217"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="686"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="222"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="249"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="686"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="696"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="102"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="122"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR002583-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="710" mass="81173" checksum="13B1C76ADBFC8B0C" modified="2011-11-16" version="1">MSDAKVETHEFTAEISQLMSLIINTVYSNKEIFLRELISNASDALDKIRYEALSDPSKLESEPELFIRITPKPEQKVLEIRDSGIGMTKADLVNNLGTIAKSGTKSFMEALSAGADVSMIGQFGVGFYSLFLVADHVQVISKHNDDEQYVWESNAGGKFTVTLDETNEKLGRGTVLRLFLKEDQLEYLEEKRIKEVVKKHSEFVAYPIQLVVTKEVEKEVPVEEEEEEKTEEGEDKKPKLEEVDDEEEGEKKEKTKKVKEQVTETEELNKTKPLWTRNPTDITQEEYNAFYKSISNDWEDPLAVKHFSVEGQLEFRAILFVPKRAPFDAFESKKKKNNIKLYVRRVFITDDAEELIPEWLGFVKGVVDSEDLPLNLSREMLQQNKILKVIRKNIVKKLIETFNEIAEDQEQFDKFYTAFSKNIKLGIHEDQQNRNALAKLLRYNSTKSSEELTSLSDYVTRMQPHQKNIYYITGESIKAVEKSPFLDALKAKNFEVLFMVDPIDEYAMTQLKEFEDKKLVDITKDFELEETDEEKAEREKEVAEFEPLTKALKEILGEQVEKVVVSYKLVDAPAAIRTGQFGWSANMERIMKAQALRDTTMSSYMSSKKTFEISPKSPIIKELKRKVEVDGAEDKTVKDLTTLLFDTALLTSGFSLDEPSNFAHRINRLIALGLNIDDDTEETAIEEIGGTTTETTTEEPAGESVMEEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV17</accession>
    <name>G3AV17_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CBM21 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_144249</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377857.1">
        <property type="nucleotide sequence ID" value="XM_007377795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV17"/>
    <dbReference type="STRING" id="619300.G3AV17"/>
    <dbReference type="GeneID" id="18870609"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144249"/>
    <dbReference type="eggNOG" id="KOG3986">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_462306_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV17"/>
    <dbReference type="OMA" id="EFPQFNE"/>
    <dbReference type="OrthoDB" id="1881at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001069">
        <property type="term" value="F:glycogen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005979">
        <property type="term" value="P:regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2440">
        <property type="entry name" value="Carbohydrate binding type-21 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005036">
        <property type="entry name" value="CBM21_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038175">
        <property type="entry name" value="CBM21_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050782">
        <property type="entry name" value="PP1_regulatory_subunit_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307:SF36">
        <property type="entry name" value="GLYCOGEN-BINDING SUBUNIT 76A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03370">
        <property type="entry name" value="CBM_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51159">
        <property type="entry name" value="CBM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM21" evidence="2">
        <location>
            <begin position="244"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="375"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="420"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="460"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="375"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="422"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="460"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="552" mass="61159" checksum="F36CFFD2B815AF09" modified="2011-11-16" version="1" fragment="single">TTMSTTITKGGPMPLTYSNLIASNLYTQREAASSSESVDSNASTIKLTSGTPPPQQLPNYYVPPKVNVQESDSQDSSSSTSMSSTCSTSSSMSSTSYVLPPRLVRKKSGELVKSSLKLPQLLQRSLSTPQLPARKSVRFASRLTNIKMFDGMESPAAVSSTASTPLHSPPSGRYDDELDDFDDELRFLKTKPKVDYFDWNWHGSMESSSSTSSDDEDEYWSTPKSLTGNSLREYRLTSHNIPKIVNFKDMIVWLPSAYVLKTDGMTYLYGLVNVKNLAFEKKIFIKLTLNNWKTSVVFGGNAIITYVKSMGDVDQFKFKINVDDLISNRYDGFNAIDLQMCIKYEVGSDVYWDNNGGANYQFKLTMVNGIKSYTYSPSPSKNSPSISETPKKAETEEFPQFNELISKLILHQQVSKSDASLARRPTIATTTAPQQPRFSFSVHNNFEDRKPQRPFLRTSYSSSDVTTAASQQQQQAGRPKYSQSFRQRQQKKDTGKPEFSSVSYADLLNNYCFANTAATAAAGSSTPTTPVLAGCLPSTASTLHSFSDSIHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVB6</accession>
    <name>G3AVB6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_144561</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377901.1">
        <property type="nucleotide sequence ID" value="XM_007377839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVB6"/>
    <dbReference type="FunCoup" id="G3AVB6">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVB6"/>
    <dbReference type="GeneID" id="18870644"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144561"/>
    <dbReference type="eggNOG" id="KOG0511">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022885_2_0_1"/>
    <dbReference type="InParanoid" id="G3AVB6"/>
    <dbReference type="OMA" id="EGARCIY"/>
    <dbReference type="OrthoDB" id="684045at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044515">
        <property type="entry name" value="ABTB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46231">
        <property type="entry name" value="ANKYRIN REPEAT AND BTB/POZ DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46231:SF1">
        <property type="entry name" value="ANKYRIN REPEAT AND BTB_POZ DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="161"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="339"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="617" mass="70791" checksum="EA35A43912EE90DA" modified="2011-11-16" version="1">MIRENPARPDSSSSTNDSVFSNNQTDATSTSSTVLPVIQQDPEVAKAFADICMACRTGDIEVVDSLLSTPNLDINQVDEYNYSPLILSSLCGHYPIVELLLQRGAVCDRDTFQGARCIYGALTDEIRDLLVSFDISKAVDSTQPFAAHIASLLNPLVTNTRDVAFQFKSASGELTSFKLHRFLLAARCPYFREQLLNDGKWAYLTVVNMPESVDPSAFKVIMDYIYLRTDSIAIDDTSIQAQLIELARKYKLPDLLTGIEKIKQCKDDKERAKVKHDLAFQFVETARGNLDEYLDKYVWNEKMVSPMDLEEDVDIDDINSEEFITEEQRSTLLEADSIPDIILSCIDSETESVVYYPVNKSILARSEYFDTMFKSDIFKYSQEDIPVYKDKIQDQEIINRPQLQSEHLPVVQITTTTTNQNVAQMILSYLYHDDVKHIPLPLTIELLLGADELFLERLKTMCAVNISSQFNSFTFDELLDLKLKLGYNAYDLIRIAWQTRCDKLEQHLTKMIAYNLHKLFQVEEEREEFAKLIEESAARIKERHETDTIELIDDIRYYLSKKYAVHTEFQSLDPAMSFRDPDAKPAEHIQLYKTALMNYDKDIEIIDYFLSELQLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVS0</accession>
    <name>G3AVS0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">DNA 5'-3' helicase</fullName>
            <ecNumber evidence="19">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">SPAPADRAFT_158868</name>
    </gene>
    <organism evidence="25">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the helicase family. RAD3/XPD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="19"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007378001.1">
        <property type="nucleotide sequence ID" value="XM_007377939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVS0"/>
    <dbReference type="FunCoup" id="G3AVS0">
        <property type="interactions" value="827"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVS0"/>
    <dbReference type="GeneID" id="18871138"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_158868"/>
    <dbReference type="eggNOG" id="KOG1131">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011312_1_0_1"/>
    <dbReference type="InParanoid" id="G3AVS0"/>
    <dbReference type="OMA" id="WQTMGIL"/>
    <dbReference type="OrthoDB" id="272481at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000112">
        <property type="term" value="C:nucleotide-excision repair factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045951">
        <property type="term" value="P:positive regulation of mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18788">
        <property type="entry name" value="SF2_C_XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.40:FF:000001">
        <property type="entry name" value="DNA repair helicase (Rad3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.20:FF:000001">
        <property type="entry name" value="DNA repair helicase rad15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000135">
        <property type="entry name" value="DNA repair helicase RAD3, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000128">
        <property type="entry name" value="Putative DNA repair helicase RAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000381">
        <property type="entry name" value="TFIIH basal transcription factor complex helicase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.20">
        <property type="entry name" value="Bacterial XPD DNA helicase, FeS cluster domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010643">
        <property type="entry name" value="HBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013020">
        <property type="entry name" value="Rad3/Chl1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001945">
        <property type="entry name" value="RAD3/XPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042493">
        <property type="entry name" value="XPD_DNA_FeS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00604">
        <property type="entry name" value="rad3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF1">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06777">
        <property type="entry name" value="HBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00852">
        <property type="entry name" value="XRODRMPGMNTD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 24" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="17" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="23">
        <location>
            <begin position="7"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="770"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="21">
        <location>
            <begin position="266"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="EGW30235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="792" mass="91189" checksum="C368B32D18685E5C" modified="2011-11-16" version="1">MKFFIDDLPVLFPYPKIYPEQYAYMCDIKKTLDVGGNCILEMPSGTGKTVSLLSLTVAYQMHYPEQRKIVYCSRTMSEIEKALIELYKLMEYRANELGYVEDFRGLGLTSRKNLCLHPTISKERKGVVVDEKCRRITNGQLKNKIEKGMISEQQQADNPEVNSLCSFHENLEDMDQHDLIPPGVYSFDGLLKYCKQRGTCPYFTVRRMMPFCNIIIYSYHYLLDPKIADRVSKELSKDSIVIFDEAHNIDNVCIESLSLDLTEDTLKRATRGANKLGDAIEEMKAQDSEKLQNEYEQLVEGLRQAEIARNEELFMSNPILPQDLLDEAIPGNIRKGEHFIAFLKRFIEYLKTRMKVLHVISETPISFLKHLKELTYIDRKPLRFCSERLSLLVRTLELAEIEDFNALKDIATFATLVSTYDTGFQLILEPYETEGSSVPNPILHFTCLDASIAIKPVFERFYSIIITSGTISPLDMYPKMLNFQTVIQESYAMTLARRSFLPMIVTKGSDQVSISSRFEIRNDPSVVRNYGSLLVEFSKITPDGMVVFFPSYLYMESIISMWQNMGVLDEVWKYKLILVETPDAQETSLALETYRKACSNGRGAVLLSVARGKVSEGIDFDHHYGRTVLMIGIPFQYTESRILKARLEFMRDHFQIRENDFLSFDAMRHAAQCLGRVLRGKDDYGIMVLADRRFSRKKNQLPKWIAQALNDSDTNLSTDMALATAKRFLRSLAQPANPKDQEGVSVWNLEQLEEYQRKYEHKVQPIESEIAEEENNQEDNFMDIDDADLDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVT5</accession>
    <name>G3AVT5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_63605</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377746.1">
        <property type="nucleotide sequence ID" value="XM_007377684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVT5"/>
    <dbReference type="GeneID" id="18874828"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63605"/>
    <dbReference type="HOGENOM" id="CLU_2559760_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVT5"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW29980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="82" mass="9017" checksum="4856B5333090B09E" modified="2011-11-16" version="1">MIKGCLSRGRKIAETIPNSSPTQIGTCHIVQRHKSDSPPPAYNMSEIPLQPAPAYLPLYEQISSQQLQSSDELDRLNSSPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVX5</accession>
    <name>G3AVX5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Iron-sulfur cluster assembly protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_63637</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 8">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; iron-sulfur cluster biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the NifU family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377786.1">
        <property type="nucleotide sequence ID" value="XM_007377724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVX5"/>
    <dbReference type="FunCoup" id="G3AVX5">
        <property type="interactions" value="575"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVX5"/>
    <dbReference type="GeneID" id="18874844"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63637"/>
    <dbReference type="eggNOG" id="KOG3361">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079283_1_0_1"/>
    <dbReference type="InParanoid" id="G3AVX5"/>
    <dbReference type="OMA" id="SMVTEMV"/>
    <dbReference type="OrthoDB" id="1925777at2759"/>
    <dbReference type="UniPathway" id="UPA00266"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06664">
        <property type="entry name" value="IscU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1010.10:FF:000005">
        <property type="entry name" value="Iron-sulfur cluster assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011339">
        <property type="entry name" value="ISCU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01999">
        <property type="entry name" value="iscU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY ENZYME NIFU HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82649">
        <property type="entry name" value="SufE/NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="6 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="NIF system FeS cluster assembly NifU N-terminal" evidence="9">
        <location>
            <begin position="44"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="193" mass="20381" checksum="93D2452419A83B41" modified="2011-11-16" version="1">MFSRSILRATSQAALRSTGARIITNPRVMIPTVSPIVSVVKRSYHEKVIDHYENPRNVGTLNKNDINVGTGLVGAPACGDVMRLQIKVDEATGRIEDVKFKTFGCGSAIASSSYATELVKGKTLDEALQIKNTFIAQELSLPPVKLHCSMLAEDAIKSAVKDYKSKRSGLKPTLGPDAVASAGVGATAAPAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADW5</accession>
    <name>G3ADW5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_57745</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372101.1">
        <property type="nucleotide sequence ID" value="XM_007372039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ADW5"/>
    <dbReference type="FunCoup" id="G3ADW5">
        <property type="interactions" value="888"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ADW5"/>
    <dbReference type="GeneID" id="18872178"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57745"/>
    <dbReference type="eggNOG" id="KOG0877">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065898_0_2_1"/>
    <dbReference type="InParanoid" id="G3ADW5"/>
    <dbReference type="OMA" id="PYEEWSD"/>
    <dbReference type="OrthoDB" id="10253125at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045903">
        <property type="term" value="P:positive regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000002">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000004">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005711">
        <property type="entry name" value="Ribosomal_uS5_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_uS5_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01020">
        <property type="entry name" value="uS5_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718:SF4">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="RIBOSOMAL S SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="S5 DRBM" evidence="9">
        <location>
            <begin position="73"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="251" mass="26978" checksum="791F66492FD52626" modified="2011-11-16" version="1">MSAAPEAPKRNFGRNARRGGRRGGRREEEKGWTPVTKLGRLVKAGKITTIEEIYLHSLPVKEFQIIDLLLPELKDEVMKIRSVQKQTRAGQRTRMKAVVVIGDSNGHVGLGIKTAKEVASAIKAAIVIAKLSIIPIRRGYWGSNLGAPHSLPTKVTGKCGSVAVRLIPAPRGKGIVASPAVKKLMQLAGVEDVYTSSSGSTRTTENTLKAAFVAIGNTYGFLTPNLWAETPLAASPLEVYAEEAAAGKKRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE83</accession>
    <name>G3AE83_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Piwi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58832</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373029.1">
        <property type="nucleotide sequence ID" value="XM_007372967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE83"/>
    <dbReference type="STRING" id="619300.G3AE83"/>
    <dbReference type="GeneID" id="18872632"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58832"/>
    <dbReference type="eggNOG" id="KOG1041">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004544_6_2_1"/>
    <dbReference type="InParanoid" id="G3AE83"/>
    <dbReference type="OMA" id="CHTFGRA"/>
    <dbReference type="OrthoDB" id="10252740at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Piwi" evidence="1">
        <location>
            <begin position="53"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="414" mass="45895" checksum="1ABB6C60B5354E08" modified="2011-11-16" version="1" fragment="single">FLGEVHRLGVNVRDGFKKYSIDVDHKSVQDQSALESALCTVFTKAKGGDKCDYLIIILPKKDTMLYRAVKRVGDLKVGVANTCVVFNIFTKKLRGSNKFDIGCYSQIAMKINLKLGGSNHRLSAAHSKGLFDAQKVPVFILGADVTHPTNTTSKEESVSIASVVGSEDGIFNKFPGSIRIQSGGQEVISEMKGMVLERLENFYIKVGKLPSKILFYRDGVSEGQYYTVLKDELPQIKAAFVQFGKSKNKPKYSPKLTFLVVVKRHHTRFIPLEENGVDANEKKVAVTSNDNVTPGTTVDRDICSPAFFDFYIQSQQALQGCGIPAHYYVLHDENGYGSDEIQTITYNLCHTFGRATKSIKTVPAAYYADLLCTRGRCYIGGIERERGRQTAIEKAKAKLGSNVATSIKNTMYYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEF5</accession>
    <name>G3AEF5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_58946</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PHAF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373155.1">
        <property type="nucleotide sequence ID" value="XM_007373093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEF5"/>
    <dbReference type="GeneID" id="18872690"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58946"/>
    <dbReference type="eggNOG" id="KOG2819">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_559104_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEF5"/>
    <dbReference type="OMA" id="HNYFRYG"/>
    <dbReference type="OrthoDB" id="411211at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005373">
        <property type="entry name" value="PHAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039156">
        <property type="entry name" value="PHAF1/BROMI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13465:SF2">
        <property type="entry name" value="PHAGOSOME ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13465">
        <property type="entry name" value="UPF0183 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03676">
        <property type="entry name" value="PHAF1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="469" mass="53820" checksum="67B80889840A111A" modified="2011-11-16" version="1">MIHETTINPGQGLGNSIKLGSPMFDIIKHLDKHKYRFRITFSEDKFYETPTLVTVVELGIRLTFNNHPDQYLDLIEVLNLDESADKKLLRLVYNGQSLNEFENEPEVTSLPLESTISKNSSSSTIMDSSPALSSTSSTNSTYQTLSSTGPTFKTIYNKIFGPTYPGKLNRVNKTYILSYPGISFKFTITDNSLLSLLDQEDDVHQILSKLLNWDNPHDVTCDSIALYKGNSWEDYKNNKRRPSTSSNELNKLAVNLNEGLVRIIKHDSEEVIKIGESTQQEVVNILGPPDEYFNKFDSRLLIHNHLTDEKESQDISHCKFHNYYKYGIDILYDLNNTKGNQNKMNTTVKKIIIHNGGIPEDLNFMKWNRCNWEIEREKEDDMPGFEINSTMYFDQLPDVFHQLNPVLLNRNESEFIDNDLDIIEMPDSVEPEEENPKVKTWGQSKLYGLNRCILEVINSNGCISTVTIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF69</accession>
    <name>G3AF69_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA repair metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_69243</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372270.1">
        <property type="nucleotide sequence ID" value="XM_007372208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF69"/>
    <dbReference type="FunCoup" id="G3AF69">
        <property type="interactions" value="214"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF69"/>
    <dbReference type="GeneID" id="18875261"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_69243"/>
    <dbReference type="eggNOG" id="KOG1361">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005260_5_1_1"/>
    <dbReference type="InParanoid" id="G3AF69"/>
    <dbReference type="OMA" id="PITANHC"/>
    <dbReference type="OrthoDB" id="262529at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16273">
        <property type="entry name" value="SNM1A-1C-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011084">
        <property type="entry name" value="DRMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240:SF6">
        <property type="entry name" value="DNA CROSS-LINK REPAIR 1A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07522">
        <property type="entry name" value="DRMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA repair metallo-beta-lactamase" evidence="6">
        <location>
            <begin position="445"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="638" mass="73871" checksum="CF2C7C6FE00603CD" modified="2011-11-16" version="1">MKASLITGKKQRSIFEFSRTRIQAPVSREIIEITSDTEDDYVRCPICELSLSNLTINDRTEHVDACLVRVTFVEEPLKPQISPTIKPDIKKRKIIRKEQPQPIDPEKLKYINKEIVTKPQGEIAIPKAITKSKTKRPIPPLKIMTFPIQESQKYQVSVDAFCYAPHDTISQYFLSHFHSDHYGGISKKWAYERVFKDDTDFENESKYRRIIYCSVITGKLLTLHFSIDPKFIKHLEIETRYQIKSYITDIDDGGYESEEQTPGLYVTPISANHCPGSTIFLFESIGLENKRTRILHCGDFRVNYEILSHPLLKQFSIDPNSICLDKVYLDTTYMSPAYNFPKQELVCETVATMFQDLMKEETKSTSLFSTWFGIGTQSRITDFWKKKFLILVGTYVIGKEKLAIAISKKLNCPIYISNIGSRNKKWDTLKTYDDQYLDSVLTTNDLGDDSSDAIIHLVPMNIVSSTQELSNYFNHNKYFEYFERCVGLRPTGWSFSPGNHREIKDPEIPNHLQELERIMSKSPSFDYLNNILTQVTSTPSKTKGKPDKQLYRIYALPYSEHSSFRELAYFVIFFNINRVIPTVNCENERSIRAMTDIIETWELARRIITNSIKPSDDDDVVVDMELIEKIQQLSLNSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFD5</accession>
    <name>G3AFD5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_53310</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372336.1">
        <property type="nucleotide sequence ID" value="XM_007372274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFD5"/>
    <dbReference type="STRING" id="619300.G3AFD5"/>
    <dbReference type="GeneID" id="18871774"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53310"/>
    <dbReference type="eggNOG" id="ENOG502SEMM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127758_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFD5"/>
    <dbReference type="OMA" id="LDCKLYG"/>
    <dbReference type="OrthoDB" id="2544694at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11578">
        <property type="entry name" value="DUF3237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="180" mass="19726" checksum="05B19F3C3B232861" modified="2011-11-16" version="1">MSLNLALPGIPKPAEFIPVFTFNLKLASDPTSIFSDPASDKALSLATVSSGEVKTVANSLGLEFEVVDISGWDDLSLKISASTNSLDCKLYGKTPSGAGVFIHYGGKVQLTESTINVVTKKTQVASIEESYVTCNPTFQLDEAVEDKYKWVLKENFFGRGRFGRDDDGTLYVQYYVYVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFG8</accession>
    <name>G3AFG8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_58082</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372369.1">
        <property type="nucleotide sequence ID" value="XM_007372307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFG8"/>
    <dbReference type="FunCoup" id="G3AFG8">
        <property type="interactions" value="165"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFG8"/>
    <dbReference type="GeneID" id="18872312"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58082"/>
    <dbReference type="eggNOG" id="KOG2655">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017718_8_0_1"/>
    <dbReference type="InParanoid" id="G3AFG8"/>
    <dbReference type="OMA" id="EASHAEI"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005621">
        <property type="term" value="C:cellular bud scar"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990317">
        <property type="term" value="C:Gin4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005937">
        <property type="term" value="C:mating projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032160">
        <property type="term" value="C:septin filament array"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010314">
        <property type="term" value="F:phosphatidylinositol-5-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030011">
        <property type="term" value="P:maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000921">
        <property type="term" value="P:septin ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000238">
        <property type="entry name" value="Cell division control 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="6">
        <location>
            <begin position="21"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="349"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="396" mass="45999" checksum="3912A4C6311F894B" modified="2011-11-16" version="1">MPEPVGITSLPDQRYKIVSKEGATFTIMVAGESGLGKTTFINTLFQTSLKPHQDPTERHNKFQSAQETVEIGIVRAILEEKNFKIRLNIIDTPGFGNNVNNNDSWTPIIDFIDDQHESYMKQERQPLRTNKKDLRVHACLYFIRPTGHSLKPLDIEIMQRLSTRVNLIPVIAKADTLSPAELDTFKTRIREIIEAQDINIYTPPLELDDPASAEHAKQLIESMPFAIIGAEEEIEISPGKFSRGRKYPWGVVEVENDEHCDFKKLRSLLLRTNMLDLVLSTNEIHFENFRSTKLGADEEENEDELKEELTNEKGEVVKKPSITKKPRRLHNPKFKEEEDQLKKFFTEQVKAEEQRFRQWETNIINERNRLNQDLEEMQLKLKNLEEQIKKLQLAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFH8</accession>
    <name>G3AFH8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_48028</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372379.1">
        <property type="nucleotide sequence ID" value="XM_007372317.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFH8"/>
    <dbReference type="FunCoup" id="G3AFH8">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFH8"/>
    <dbReference type="GeneID" id="18871167"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48028"/>
    <dbReference type="eggNOG" id="ENOG502S3U5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052194_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFH8"/>
    <dbReference type="OMA" id="DSENWID"/>
    <dbReference type="OrthoDB" id="5551751at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040200">
        <property type="entry name" value="Mug57-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28156">
        <property type="entry name" value="FAS1 DOMAIN-CONTAINING PROTEIN YDR262W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28156:SF1">
        <property type="entry name" value="FAS1 DOMAIN-CONTAINING PROTEIN YDR262W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442466" description="FAS1 domain-containing protein" evidence="3">
        <location>
            <begin position="18"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="293" mass="33488" checksum="160DDBA3E68F63CD" modified="2011-11-16" version="1">MKLTYITSLTLISSVVAKNLVDLNSFKAEVEEDKSQLEKRKNVVNLDDFKILNGKREPKNVIDINKFKEPITKRKNVVNLNDFKIDKRDAKNVFDLTKLQQGSQRPSKRDEQKVLANNLLFTIDSVDCYNNLLQSILPQIRSISIFAGYIRDNQVLNDKTEFVNETMVIIAPMDSAIESKLSGLKPWEFPHSLGNVKDQFEQEKLLQENLNGFLNGHIITNFESKLIIGSDDDVKLQQVVIAPLNNGKLIKIKQDSITDKFKVKVDREWIDVVLVKQVENGFVFVIDDSLVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFV7</accession>
    <name>G3AFV7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HIT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_58239</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372508.1">
        <property type="nucleotide sequence ID" value="XM_007372446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFV7"/>
    <dbReference type="FunCoup" id="G3AFV7">
        <property type="interactions" value="92"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFV7"/>
    <dbReference type="GeneID" id="18872378"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58239"/>
    <dbReference type="eggNOG" id="ENOG502S27I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1619339_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFV7"/>
    <dbReference type="OMA" id="QYNTVKF"/>
    <dbReference type="OrthoDB" id="18412at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23024">
        <property type="entry name" value="zf-HIT_ZNHIT2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
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        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HIT-type" evidence="2">
        <location>
            <begin position="3"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="140" mass="16032" checksum="871D8FAF979FFE47" modified="2011-11-16" version="1">MICGLCQEQESKYKCPKCKVPYCSIACYKSEAHKHEEKESNQSTPKVNIAIKQEDITDNKYASVINDPQIKSMLREPALQVHLLTISKLLNEDSLIQGNVNYDQRLELVRLKLQDLRAGGIEENELVEEFIGRVLQLLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFZ7</accession>
    <name>G3AFZ7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Inorganic phosphate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_58324</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372548.1">
        <property type="nucleotide sequence ID" value="XM_007372486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFZ7"/>
    <dbReference type="FunCoup" id="G3AFZ7">
        <property type="interactions" value="848"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFZ7"/>
    <dbReference type="GeneID" id="18872401"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58324"/>
    <dbReference type="eggNOG" id="KOG0252">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_14_1"/>
    <dbReference type="InParanoid" id="G3AFZ7"/>
    <dbReference type="OMA" id="DKMWRVV"/>
    <dbReference type="OrthoDB" id="433512at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097079">
        <property type="term" value="F:selenite:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097080">
        <property type="term" value="P:plasma membrane selenite transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006797">
        <property type="term" value="P:polyphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17364">
        <property type="entry name" value="MFS_PhT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000421">
        <property type="entry name" value="Inorganic phosphate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004738">
        <property type="entry name" value="Phos_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00887">
        <property type="entry name" value="2A0109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="494"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="58"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="550" mass="60026" checksum="316243E70D072752" modified="2011-11-16" version="1">MTNDIHETGHVHVSSAGGKTAFKDYIGQFADVEDPLERRRLALEEIDRAGFGWTQMKMIMIAGVGFMTDSYDIFAINLGINMLQYVFWNGTIPASTTTLIKVSTSVGTVIGQVGFGSVADHVGRKAIYGLELIVMIFATVFQCTLGESPAINFTAIFCTMRILMGIGIGGDYPLSSIISSEFSTTKWRGAIMAAVFSNQGIGQVFAGIVAMILVAAYRSDLEPAQTGAECIGNCRRACDQMWRILIGFGCVPGVIALYYRLTIAESPRYALDVVDHDVVQKVADAEASIAAEAKEIVAPKASFRDFVRHFGQWRYGKILLGTAGSWFMLDVAFYGLGLNTTTILQTIGYAGQANVYEKLYNSAAGNLIIVCAGSLPGYWFSIATIDILGRRVIQLGGFILLTIILCIMGFAYHRLGNHGLLAFFVLAQFFQNFGPNVTTFITPGEIFPTRYRSTAHGISAATGKVGAIIAQTCIGTLVNHGCAKDEPNCFLPHVLEIFALFMLLGIFTTLLLPETKRMTLEEICEKYHGEIDNTKLTREVYATESSDEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGA9</accession>
    <name>G3AGA9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_132639</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372660.1">
        <property type="nucleotide sequence ID" value="XM_007372598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGA9"/>
    <dbReference type="STRING" id="619300.G3AGA9"/>
    <dbReference type="GeneID" id="18869675"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132639"/>
    <dbReference type="eggNOG" id="ENOG502RQ80">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122462_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGA9"/>
    <dbReference type="OMA" id="MKLARIH"/>
    <dbReference type="OrthoDB" id="4092895at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="194" mass="22861" checksum="B9886AEBBE014ADD" modified="2011-11-16" version="1">MYRLLARQSSIRISLLLKTTSIRYYGSNTNNKSFFKSAAKGFNIFRNIESQEFQEEYQKTAKTEPLTDQLMKAIGNKKEPLYLLALFHSECKKMGIVPDAPELRNVPSWKFAFNYVFKLNPIHRTFWEISRRLDVSHIHHTLYYDVHNLGILDPRNFPAELYNELQTGIYNGLDFRNVQLSVNIHPQWTEKKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGK6</accession>
    <name>G3AGK6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Decapping protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_58573</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DCP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372757.1">
        <property type="nucleotide sequence ID" value="XM_007372695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGK6"/>
    <dbReference type="FunCoup" id="G3AGK6">
        <property type="interactions" value="148"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGK6"/>
    <dbReference type="GeneID" id="18872509"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58573"/>
    <dbReference type="eggNOG" id="KOG2868">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113008_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGK6"/>
    <dbReference type="OMA" id="IWIHTVA"/>
    <dbReference type="OrthoDB" id="440673at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098562">
        <property type="term" value="C:cytoplasmic side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098745">
        <property type="term" value="C:RNA decapping complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09804">
        <property type="entry name" value="Dcp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010334">
        <property type="entry name" value="Dcp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290:SF0">
        <property type="entry name" value="DECAPPING PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290">
        <property type="entry name" value="TRANSCRIPTION FACTOR SMIF DECAPPING ENZYME DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06058">
        <property type="entry name" value="DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW35345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="179" mass="20758" checksum="714F4F7D3C5974DF" modified="2011-11-16" version="1">MGELNNQEPITPEEALKLYTNTLNFNVISRYDPGIKQLLCHTSHCVVYKFNDESQEWIKSDYSGTLALYVRDFKVPPSNKQLSYQDLQQLFCYGLILLNRNNPDCFSLGLLPNKITKHYFPNGLNDSSVSEMDVELNDNLIIVRNLLGEIYGLWVFNEEDRIKLFKSIEFCLNSDAKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGL8</accession>
    <name>G3AGL8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Oligopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_48358</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the peptidase M3 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372770.1">
        <property type="nucleotide sequence ID" value="XM_007372708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGL8"/>
    <dbReference type="SMR" id="G3AGL8"/>
    <dbReference type="FunCoup" id="G3AGL8">
        <property type="interactions" value="746"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGL8"/>
    <dbReference type="GeneID" id="18871210"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48358"/>
    <dbReference type="eggNOG" id="KOG2089">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001805_1_2_1"/>
    <dbReference type="InParanoid" id="G3AGL8"/>
    <dbReference type="OMA" id="KNFQSAM"/>
    <dbReference type="OrthoDB" id="534666at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06455">
        <property type="entry name" value="M3A_TOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000074">
        <property type="entry name" value="Metalloprotease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.40:FF:000001">
        <property type="entry name" value="Thimet oligopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.40">
        <property type="entry name" value="Endopeptidase. Chain P, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.10">
        <property type="entry name" value="Neurolysin, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024077">
        <property type="entry name" value="Neurolysin/TOP_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024080">
        <property type="entry name" value="Neurolysin/TOP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804:SF84">
        <property type="entry name" value="SACCHAROLYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M3A/M3B catalytic" evidence="10">
        <location>
            <begin position="219"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW35358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="683" mass="78998" checksum="09FE079284278B20" modified="2011-11-16" version="1">MSIDFEALTAKQNFPLWEQTPEDVLKTANELIEKQKNLFDKIAATEEPTLDNLLKPFAELNNKDAFPESQVTFYQYVSTNKELRDASTKAEELIEESSITEWSRPEVYKVFQKLYDAVKDTDIDPESKRYLKKSVTAFKRNGLALPEETREKIKKLKVELSNLSVQFAKNLNEEKGFVLFTKKELDGVPEDVIGSYSKAEQDGEEKLKVTFKYPDILPLLKHAHNGNTRKTAYIAYNNRCSENAPILEQIIKVRYELGQLLEYDTYSDFVLEERMAKKKEVVLDFLSDLRTKLTPVATVELEELLKLKNDDLKSRGLEPEDKVYAWDSAYYNELLLEKKYSVDNIKIAEYFPLESTVNKMLGFYETIFDIKFVRIEKPAKGAVWHEDVQQFAVYKNIKHGEPRQEFIGWIYFDLHPREGKYGHAANFGLGPGYLEDDGKTRHTPVTALVCNFTKPTPEKPSLLKHDEVTTFFHELGHGIHNIISQTRYARFHGTRVERDFVETPSQMLEYWTWSKDEIKGLSSHYKTGEPIDDDLVDKLIKTKHVNTGIFNLRQLFFGIFDMQVHTIKDKKDLDELDLFKKWNSLREDITLISSDNNPTKGYSSFGHIAGGYESGYYGYLYSLVYAADIYYTLFKKDPMNVESGIRYRDIILTRGGSRDSIDNLKELLGRAPNAQAFLQEIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGR3</accession>
    <name>G3AGR3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I, II, and III subunit RPABC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_58612</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the archaeal Rpo10/eukaryotic RPB10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372808.1">
        <property type="nucleotide sequence ID" value="XM_007372746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGR3"/>
    <dbReference type="FunCoup" id="G3AGR3">
        <property type="interactions" value="534"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGR3"/>
    <dbReference type="GeneID" id="18872533"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58612"/>
    <dbReference type="eggNOG" id="KOG3497">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143122_2_1_1"/>
    <dbReference type="InParanoid" id="G3AGR3"/>
    <dbReference type="OMA" id="YCCRRMF"/>
    <dbReference type="OrthoDB" id="10258858at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000024">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00250">
        <property type="entry name" value="RNApol_arch_Rpo10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023580">
        <property type="entry name" value="RNA_pol_su_RPB10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020789">
        <property type="entry name" value="RNA_pol_suN_Zn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000268">
        <property type="entry name" value="RPABC5/Rpb10"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003089">
        <property type="entry name" value="PRK04016.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431:SF3">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01194">
        <property type="entry name" value="RNA_pol_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005653">
        <property type="entry name" value="RNA_pol_N/8_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46924">
        <property type="entry name" value="RNA polymerase subunit RPB10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01112">
        <property type="entry name" value="RNA_POL_N_8KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="72" mass="8574" checksum="0CA4B2E7F4E3515E" modified="2011-11-16" version="1">MIIPVRCFSCGKVVGDKWETYLEYLQDEAMTEGDALDKLKLKRYCCRRMVLTHVDLIEKFLRYNPLEKKEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGW3</accession>
    <name>G3AGW3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1,3-beta-glucan synthase</fullName>
            <ecNumber evidence="3">2.4.1.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">1,3-beta-D-glucan-UDP glucosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">GLS3</name>
        <name evidence="13" type="ORF">SPAPADRAFT_144914</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;3)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;3)-beta-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21476"/>
            <dbReference type="Rhea" id="RHEA-COMP:11146"/>
            <dbReference type="Rhea" id="RHEA-COMP:14303"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37671"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.34"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 48 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.34" evidence="3"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372048.1">
        <property type="nucleotide sequence ID" value="XM_007371986.1"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGW3"/>
    <dbReference type="GeneID" id="18870667"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144914"/>
    <dbReference type="eggNOG" id="KOG0916">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000844_0_1_1"/>
    <dbReference type="InParanoid" id="G3AGW3"/>
    <dbReference type="OMA" id="WCPENGA"/>
    <dbReference type="OrthoDB" id="1880850at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000148">
        <property type="term" value="C:1,3-beta-D-glucan synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003843">
        <property type="term" value="F:1,3-beta-D-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006075">
        <property type="term" value="P:(1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051278">
        <property type="term" value="P:fungal-type cell wall polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026899">
        <property type="entry name" value="FKS1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056261">
        <property type="entry name" value="FKS1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003440">
        <property type="entry name" value="Glyco_trans_48_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741:SF48">
        <property type="entry name" value="1,3-BETA-GLUCAN SYNTHASE COMPONENT FKS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741">
        <property type="entry name" value="LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14288">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23605">
        <property type="entry name" value="FKS1_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02364">
        <property type="entry name" value="Glucan_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01205">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="326"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="369"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="398"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="441"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="499"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="526"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1161"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1194"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1252"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1278"/>
            <end position="1296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1357"/>
            <end position="1382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1412"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1440"/>
            <end position="1461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1467"/>
            <end position="1494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1546"/>
            <end position="1565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1599"/>
            <end position="1623"/>
        </location>
    </feature>
    <feature type="domain" description="1,3-beta-glucan synthase component FKS1-like" evidence="12">
        <location>
            <begin position="130"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1651" mass="190516" checksum="4D55F24178DD69E4" modified="2011-11-16" version="1">MEESKFKSWNLDNGSTLSEQEILNIFVELGDMFGFQNDNVNNMFDHFMTQLDSRSCRMRCPTALLSLHLDYIGGKNSNYKKWFFAAQFNFDYDITWNPKKSIKKKKNRNSIEEESNESKWARRFHGCTDSDYVYHIALYLLIWGEANNVRFMPECICFIFQSAFDYWQYQRSILPTDKDQQENIGLPQFHFLDQIITPIYNFIRDQQYCKAEGGGWQRKETDHANTIGYDDINQQFWSPKGLYKIKLRDGRRLYSLPKEERYMKVGEINWDKAFKKTYRERRTWLHVITNFNRVWIVHVSVFWYFMSLNSPSLYTPDYVPNKEPQMHVRLAIMSLGGAIASLLSLAGDICEYFFVPRRVPNKQQLWHRIVFLVILTILNISPSIYTLGFLYWDQYSHNGIVIGSISLTFSILTFLYLSIASPGDFPGTGANNTFSGSFPRLKLRSRIFSCLLWICVFVAKYSESYFFLILSMKDPIQILSTIVLNCSEGNFICKFQPKLALVILYLTDLILFFLDTYLWYVICNCLFSVGLSFSLGVSIFTPWRNIFCRLPERLSSKIYSQENGGSSDLNLSQIWNTIMFSLYREHLLSVEQVSKLVYQRGINNQNPIRPLFFVYADDNTFQLHDFFTIGKECERRITFFAQSLSSPLPEPFEVVAIPTFTVLIPHYNEKILINLEELISHSALSKLTLLDYLKQLYPSEWEAFVKDSKMLETIDIDDDDIIPMLNTEMKDVSKQVNLTINSAELPLYCLGFKDETPENILRTSIWATLRCQTLYRTVSGFMNYETALKVLYKIEDLGFNSEDHNEAELEEFASRKYNLLVAMQNLENSVPLNKDAETLFRAFPTLKVAHLEKVKINDEVTEYYSTLLDVSRTDPEGKLWRKYRIKLSGNPILGDGKSDNQNHSIIFYRGEYIQVIDANQDNYLEECLKIKSLLSEFEEINIDIGNGYDPAARDTQEDSNPVAILGAREYIFSQNIGILGDIAAGKEQTFGTLFARTMGEIGSKLHYGHPDFLNGIFMTTRGGISKAQRGLHLNEDIYAGMTATCRGGRIKHCDYYQCGKGRDLGFESIINFTTKIGAGMGEQLLSREYFYLGTKLPIDRFLSFYYAHPGFHINNLSIMLSVKMFMLLVANLGALNYGTISCEAGDNPTRGCHDLGPVLNWIDRFVLSVFVCFFISFLPLIIQELIEKGFIKAIYRIIFQVISLSPFFEVFVCQIYFKSLRDNLIFGEASYIGTGRGFAISRIAFSKLYSQYAGSSIYYGCEIFLVILFASLTMWRKALVWFVITIVSLCLAPFLFNPHQFSMSDFFIDYGNYIKWLSSGNSSKTIKMSWIHHIKTKRTKLTGAKKGSYSSGRDSSLFNLLTGEVFSPFITTLLYLCPYLFINNEDNYLGLNLKNPFLKIALITVAPYLENAVVLLVLWPLSIVLAPFLGVCYKSLPAAIAAIAHVISILVNIANFEFLFFLEDWNIVKLISASLVVLNLHRVIGHFILIVFVSRERTDNITDNISNKAWWSGRWIGNGLGFSVVTQHIREFLVKTLELNKFTLDFMIGHFLLIAMFPILFIPYIDRWHTSMLFWLKPTRQISRPVFGKRERRRQKWQIFWNFLLFIVTALFLVAVIIVPPIIDSKILKLKSTLTPPVSDIIDPDLYKDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGY1</accession>
    <name>G3AGY1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein MGR2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_53095</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MGR2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372066.1">
        <property type="nucleotide sequence ID" value="XM_007372004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGY1"/>
    <dbReference type="FunCoup" id="G3AGY1">
        <property type="interactions" value="243"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGY1"/>
    <dbReference type="GeneID" id="18871757"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53095"/>
    <dbReference type="eggNOG" id="KOG4096">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142435_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGY1"/>
    <dbReference type="OMA" id="PSNWEKF"/>
    <dbReference type="OrthoDB" id="5409308at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018450">
        <property type="entry name" value="Romo1/Mgr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28525">
        <property type="entry name" value="REACTIVE OXYGEN SPECIES MODULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28525:SF1">
        <property type="entry name" value="REACTIVE OXYGEN SPECIES MODULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10247">
        <property type="entry name" value="Romo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01378">
        <property type="entry name" value="Romo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="11654" checksum="7650B2ACB061C985" modified="2011-11-16" version="1">MPPVQQYQYAHQPSNWDKFKMGAIMGSTVGVCVGVLFGGFSILQNGAGPNGVMRTLGQYILGSVGTFGLFMSIGSVIRSDSDFQAYQQSMMYAQRAKLRAIYRDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGZ2</accession>
    <name>G3AGZ2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_144982</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372077.1">
        <property type="nucleotide sequence ID" value="XM_007372015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGZ2"/>
    <dbReference type="GeneID" id="18870668"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144982"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_12_1"/>
    <dbReference type="InParanoid" id="G3AGZ2"/>
    <dbReference type="OMA" id="PSNRYRI"/>
    <dbReference type="OrthoDB" id="508119at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000313">
        <property type="entry name" value="MFS quinate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF8">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="225"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="432"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="462"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="498"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="52"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="586" mass="64398" checksum="C424CFA051636419" modified="2011-11-16" version="1">MSHSILSNEHKELKDQVEVEPISSPPPKRKFRWTIDKRDHPKQIFNITLYLSIFVFGILGAARGLDEGNISGNITLPSFIARFGLKDPTKTTDYLANLKSNITSMVQLGAIGGALIAMKTVDYFGRVRSLQIICLIWIVGTIIQITASNVGQLYAGRLIEGLAIGHTTSIGPVYLAEVAPSPIRGLASCIFAGAVYLGVLISYMTNYGCVLHVADTVNGHINDNQWRYTLIPKILLAGLLLIMATLLCVESPRWLLKVNKVDQAVKNLSKLRHMPEDHAYIVAEISDINEQVMAEKDAIANSSILGNFRDIFTNKSIAYRFFCIAGAAQVIGQWSGANAVTIYASELFSLAGIKGPIGKLKMSAILGAVKLTSAYLGAFFVIDVFGRKRALYSGISLQMISILYYAIFLTIVPQAAEDDTELSPSQTRASKGALAAIFLSGTGWTIGFNSVQYLLGSEIFPLHIRSFAQSLVMVLHFANQYGNSKALPKLMLALQPYGAFYFFVGVMVLGLVFAFLLPELQGRSLESVEEVFTLPWYKLRNCDKLVPDHSNVHEINYINSRGKLEYNSGKVDEIVLPADQKGDRIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH97</accession>
    <name>G3AH97_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_64638</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372939.1">
        <property type="nucleotide sequence ID" value="XM_007372877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH97"/>
    <dbReference type="GeneID" id="18874988"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64638"/>
    <dbReference type="HOGENOM" id="CLU_598742_0_0_1"/>
    <dbReference type="InParanoid" id="G3AH97"/>
    <dbReference type="OrthoDB" id="10495456at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="457" mass="54770" checksum="10D1D514CB63B4BC" modified="2011-11-16" version="1">MKLIQSALFRLSFSKFPHHKGFLQVRLNSHHVTNHALNDHFTSPGLIEHGESLMDDASIDDLTQWLVKQNSKRRRKANRIQNTINRLTKQFTKQEAEIEKLRTRITRQIDYSRGIYTKLGWFIAPSRSFNHRTLDALNELFDQRGFTKDTPQQEVQQVVQDYYEVHTGNEVAKSKNKFTVDHDLKVRIGRYIIKDSSKSRQFILWNHYLYTRLSELTSDITDLAKVRMVADEWVAMFQQRTKYWYTYYHSHKRTIISVSKEIDLALRTSKFKLRPVPKGISRIRCTNSKGINSLKDTVTLAHAWDYYHRLKRPQFESESSFGFNFSLDSHEFKKFIDKLVRDRWLELSSKEKEAYRSHYERILSGKVEYEDCFKSQDKLTILGKDWYTFDYYSVVSTGKLWQLENQWKFMNSEKREKITQEFKRYLLSGGKEFRRKKLLRLEKYFGRRNQLQGIHEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHE0</accession>
    <name>G3AHE0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mitotic spindle checkpoint protein BUB3, WD repeat superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_148521</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373687.1">
        <property type="nucleotide sequence ID" value="XM_007373625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHE0"/>
    <dbReference type="STRING" id="619300.G3AHE0"/>
    <dbReference type="GeneID" id="18870789"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_148521"/>
    <dbReference type="eggNOG" id="ENOG502QPI7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_513889_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHE0"/>
    <dbReference type="OMA" id="HITHFQL"/>
    <dbReference type="OrthoDB" id="20669at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991:SF12">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="WD40 repeat-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="406"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="561" mass="63876" checksum="1C7D0237EAFB2266" modified="2011-11-16" version="1">MALRLSPEGAAVTEIILSASESQPRPILQAYASDDNAECSDYEDEQQEENDTEEQEVVIEVQEQQGGIATNSIQSFHEYELTQSESLTFLYNLLYDDNSQLSKSDILIILNSLKIREDKLPFSFESSDRGDIQGVKWPHGLKQKFNNDRRRVGKDNWFHNIPNSRENALKKLNVKGYQIKTEFFKFHKFFSKLKLHITHLQLRNLVCCGTDIGNGIYYPSSYYHDSHMQTVSVNDGDQDYHSFFRINRLMPDKIFSGKSHAMKVDCLIDSRNLSRNSNSRISTLACTNNYLASGTFEGSYILSDVSNPEQVRNIGEYHLSSNIDGITNQIIINERDQELIISSNDKSMRLIDLNKHRITQLHTLPFPLNCSALNPTNSNELFVTGDNINSYIVDKRIKFSEKHLEFKSHDDFGFGCDWSPRDENLLLTGNQDSCVKLWDRRNPDKPLYSWSGTLGFSQGIYGAPVRNCKFSHNGDYIAWAESLDHVGVVEVNDLLQTGETGIQSRIQSIDFLGKCCGMNFAPVEHGHGEQLIIGVIDCPLGGILSYKLESRDKSLDFDYYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHK7</accession>
    <name>G3AHK7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59607</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373755.1">
        <property type="nucleotide sequence ID" value="XM_007373693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHK7"/>
    <dbReference type="GeneID" id="18872982"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59607"/>
    <dbReference type="HOGENOM" id="CLU_1897517_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHK7"/>
    <dbReference type="OrthoDB" id="4036671at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="134" mass="15534" checksum="124476F911618CA5" modified="2011-11-16" version="1">MMSQQRQQAMFQNMQQQHIQQQQQQQQQQAQVGARLLDDALLEGVFDFNKDEPINPAQQQYNTTIQLQQERIKFIQSQQNQHQQQQQPQQMQSQPQQGSQGIPPDNFDIKEESTNDYIIGTDWLSSMGSGPGFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHS4</accession>
    <name>G3AHS4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SAM-dependent MTase RsmB/NOP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_49297</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373822.1">
        <property type="nucleotide sequence ID" value="XM_007373760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHS4"/>
    <dbReference type="FunCoup" id="G3AHS4">
        <property type="interactions" value="1313"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHS4"/>
    <dbReference type="GeneID" id="18871324"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49297"/>
    <dbReference type="eggNOG" id="KOG2198">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005316_4_3_1"/>
    <dbReference type="InParanoid" id="G3AHS4"/>
    <dbReference type="OMA" id="QLFTEYV"/>
    <dbReference type="OrthoDB" id="6093671at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016428">
        <property type="term" value="F:tRNA (cytidine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070301">
        <property type="term" value="P:cellular response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002127">
        <property type="term" value="P:tRNA wobble base cytosine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057285">
        <property type="entry name" value="Pre-PUA_NSUN2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057286">
        <property type="entry name" value="PUA_NSUN2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023270">
        <property type="entry name" value="RCMT_NCL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="RsmB/NOL1/NOP2-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22808">
        <property type="entry name" value="NCL1 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22808:SF1">
        <property type="entry name" value="RNA CYTOSINE-C(5)-METHYLTRANSFERASE NSUN2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25376">
        <property type="entry name" value="Pre-PUA_NSUN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25378">
        <property type="entry name" value="PUA_NSUN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02011">
        <property type="entry name" value="RCMTNCL1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="12">
        <location>
            <begin position="58"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="414"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="665"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="414"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="430"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="671"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="175"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="689" mass="79166" checksum="352AB9801ECFFD65" modified="2011-11-16" version="1">MGKRNFRKGKGGGKKNFGNRNTDADREAPWSDAVRESEVWEKYYKALNIIPEDEWDTFKQHCQENLPLTFRITGSRKHAEEIRQQFITKHVEKLKDAEFEGVKLTPKNLAYYPNQLGWQLDVSKAVIRKQKEFAETQRFLVIETQVGNISRQEAVSMIPPLLLDVQPNHYVLDMCAAPGSKTAQLVEALHSQDEKALPTGFVLANDSDYKRSHMLVHQVKRLNSPNFMVVNHDATLFPRIKLNGANEYLKFDRILCDVPCSGDGTMRKNVNVWKDFRIGNALGLHPLQYNILNRGLQLLKKGGRLVYSTCSLSPVENEAVVAAALRKWGNQIRLVNVENELPGLVRRKGINDWKVFGKDMELKERGAEGIPDSVFPPSEEEVKEFNLDYCVRVYPHLQNTGGFFITVFEKVDPEENNNKRPAEEETEEQEEKKQKVDSEIVHKPQKKDKLPRDANEEPFIFLDPNHPELAKCWPFYDFQDSFPKDCTLVRNSTGEPLRTIYYVSPIIRDILSIEEQKLKIIQAGIRLFVAQRNDLGTCPWRVQTESLHTIERYLGPKRHLSCNLQLLEVLFQEAFPKIEDLKKRGIDEEFSRRLDEMEQGCAFLTVKRGEGLEDLFLPLWKGISNVNLMVNKQDTHELLYRVFGIHTSAKDEGKEKLYQQKVEAEKRARSESAAPSEAVAESTAEAESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHX4</accession>
    <name>G3AHX4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA replication licensing factor MCM2</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">DNA replication licensing factor mcm2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Minichromosome maintenance protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">MCM2</name>
        <name evidence="21" type="ORF">SPAPADRAFT_148936</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373872.1">
        <property type="nucleotide sequence ID" value="XM_007373810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHX4"/>
    <dbReference type="FunCoup" id="G3AHX4">
        <property type="interactions" value="991"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHX4"/>
    <dbReference type="GeneID" id="18870804"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_148936"/>
    <dbReference type="eggNOG" id="KOG0477">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000995_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHX4"/>
    <dbReference type="OMA" id="TYERVTT"/>
    <dbReference type="OrthoDB" id="844at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140445">
        <property type="term" value="C:chromosome, telomeric repeat region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17753">
        <property type="entry name" value="MCM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000002">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000138">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008045">
        <property type="entry name" value="MCM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF44">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12619">
        <property type="entry name" value="MCM2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01658">
        <property type="entry name" value="MCMPROTEIN2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="20">
        <location>
            <begin position="501"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="714"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="66"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="78"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="719"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00074927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00078186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EGW34288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="867" mass="97837" checksum="14E180A0290EB1A3" modified="2011-11-16" version="1">MSNQPRNSRKRSAFEDEEDDERYNAGSSPMLASSPQLPPSSPAIPFDADELDEEDEIHNDVADLNPSDEEDGDDLMENLERDYRVNPEQDHYNLGDENIDDEGDYDELDAATRRRIDDQLNRRDAILNRANRSRRQAFLDDEGAEDQDDEIMDQYGLPIQRRRRRFQQQDEDQDDFMDDVEIDPFNEELSLESLTDIKAPSITEWILQPAVSRSIARELKSFLLEYTDENGRSVYGARIRTLGEVNAESLEVSYNHLADSKAVLALFLATSPQEMLKIFDIVAMEATELHYPNYSQIHQEIHVRIINFPNLLNLRDLRENNLNQLIKVSGVVTRRTGVFPQLKYVKFDCLKCGTVLGPFVQDANTEVRISFCTNCQSKGPFKMNSEKTLYRNYQRITLQEAPGTVPAGRLPRHREIILLSDLVDVAKPGEDIEVVGIYKNNYDGNLNAKNGFPVFATIIEANSIKRRETSAFMGGIDSNLVTLWTEEEEREFRKLSHEKGIIEKIISSMAPSIYGHKDIKTAVACSLFGGVPKNVNGKLSIRGDINVLLLGDPGTAKSQILKYLEKTANRAVFATGQGASAVGLTASVRKDPITREWTLEGGALVLADKGTCLIDEFDKMNDQDRTSIHEAMEQQTISISKAGIVTTLQARCAIVAAANPNGGRYNSTLPLSQNVDLTEPILSRFDILCVVRDLVNPESDERLASFVVDSHMRSHPTNEIEDDEDDDTAATAQRSRSAKINDLNKQKEQEISPIPQDLLVKYIQYARVKVQPKLHQMDMDKVARVYADLRRESITTGSFPITVRHLESILRIAEAFAKMRLSEFVSQNDLNRAIKVSIDSFVGAQKVTVRKQLQAKFQKYTLPTRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI11</accession>
    <name>G3AI11_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_134615</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373909.1">
        <property type="nucleotide sequence ID" value="XM_007373847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI11"/>
    <dbReference type="STRING" id="619300.G3AI11"/>
    <dbReference type="GeneID" id="18869839"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134615"/>
    <dbReference type="eggNOG" id="KOG2326">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023247_0_0_1"/>
    <dbReference type="InParanoid" id="G3AI11"/>
    <dbReference type="OMA" id="DIRGFMD"/>
    <dbReference type="OrthoDB" id="30826at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043564">
        <property type="term" value="C:Ku70:Ku80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006164">
        <property type="entry name" value="DNA_bd_Ku70/Ku80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604">
        <property type="entry name" value="KU AUTOANTIGEN DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604:SF4">
        <property type="entry name" value="X-RAY REPAIR CROSS-COMPLEMENTING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02735">
        <property type="entry name" value="Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00559">
        <property type="entry name" value="Ku78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SPOC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="5"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW34325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="644" mass="73517" checksum="19C014CA2595333D" modified="2011-11-16" version="1">MSKEYTIFALDASKSMGRHSSHPEKSDLTLASDFVFDYLNRQLVKNRKSDRFALVLFSGHNVQILYENKPLSLAISREYNSKVLQFYYDDAKVENQDAKYQFSDALKLALEQFQISAHLKFSRNLFVLTNGELPFNSFVTSDWSDYHDWVENYNITVTLGIINYTSGIKKSENKQGNERMCKEISEFGDRYLLYDMDTLLKARPPLKLIGPRVLCSAKLSFAGDAAQYWKLDLDDSGAVSMEIQVYPAIKLDKFAHGHEYYMDRENETLTQVKRSTTYYIKKTSDTNEFQNDESDEVDDLIKEEETIPVSSFDCTPGFKFSPRDILAVNSDLQEVATLISKPSIDILGFIKKEKFPVAYHTDESLFVLPSSHMGGKNKSSFDCLVQALLELESIAIVRYTQYKDSEVKLCAAYPSKVKLDGSSARVLLLIRIAMKEDEKIGRFPSLTKPNENMQSDNNETSKKIDRVAADNLMEQLIKSKKLKDEKILKDTLDNRKVGLLRSESIGTPVTDGTGLSELLMSSNPGGRKFNHYLSKIISKSLSTKSLFNFLNEEKFVENYLSKDESTVLFTTQNVLDSSLIYTGEETKENSITEKLRKELDVEYITQRKESNRKAGRKYDSIYGQDPKNDANFDEYFDIDDLLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI64</accession>
    <name>G3AI64_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AP-1 complex subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_135394</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373962.1">
        <property type="nucleotide sequence ID" value="XM_007373900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI64"/>
    <dbReference type="FunCoup" id="G3AI64">
        <property type="interactions" value="680"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI64"/>
    <dbReference type="GeneID" id="18869886"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135394"/>
    <dbReference type="eggNOG" id="KOG1062">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003824_0_0_1"/>
    <dbReference type="InParanoid" id="G3AI64"/>
    <dbReference type="OMA" id="AICAMRI"/>
    <dbReference type="OrthoDB" id="28053at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099638">
        <property type="term" value="P:endosome to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048203">
        <property type="term" value="P:vesicle targeting, trans-Golgi to endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050840">
        <property type="entry name" value="Adaptor_Complx_Large_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017107">
        <property type="entry name" value="AP1_complex_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008153">
        <property type="entry name" value="GAE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22780">
        <property type="entry name" value="ADAPTIN, ALPHA/GAMMA/EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037094">
        <property type="entry name" value="AP1_complex_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50180">
        <property type="entry name" value="GAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="GAE" evidence="10">
        <location>
            <begin position="701"/>
            <end position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR037094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="818" mass="90070" checksum="9D8DD2DAD64BF8A3" modified="2011-11-16" version="1">MGSLRTFIKSVRKAKTIADERAVIQKESAAIRTSFRDVSLDQTTRRINISKLVYLYIMGEKTHFGQVECLKLLASPRFADKRLGYLACMLILDENQEVLTLLTNSLDNDMQHPNAFVVGLALTCLGNIASPELARDLHADVEKILHSKNFYLKKKACFVAAKLVEKDPDLAEYFADKINDLINEKQPAVLLGTLRLIESLYLSSEPEQRMALLKTIPKIVGHLKRVTTSGYQPDYDVMGTSDPFLQVALLSTLRTLATDESCPAQYLEEINDILTQVASNIDSGKNAAHAILYECVKTIFSIQSDQSLKILGVNLLGKFLSTKDNNTRYVALDTLLAVINIEPLAVQRHRSTIVDCLSDGDISIRRRALELSFGILNEQNIRVLVREILTFLENCHDQELKPYITSQLTIAANKYSPNEKWHFDTLMRMLKVSGNSLTSDIVSNILALILQCNDGELKKHIVSKLFSLCLEDPEQYCLALITVWTLGEYGDLILGSTVEVNSKNVQVTEAAIVQLIEDLINKSTYSESETVQLVSYVLTAVIKLSIKFRDAQIIERLRLIINSRTRDNNLEIQVRAVEYQEIFAQDATLKKGLLARMPAPPIKQRESLTLNKTTGAKPKRAGGVASALGGTDDLLLDLMDDNNDTNTAHAAEPQQDVLMDIFGGSGAGQAQSTTTATNSNAAILDLFNAPPASAVGIGSSPSVSEIEAFTNSYIHVSFTPKSFPKDGHAIMEAVIRSNTNETISQFQLLIAVPKTQKLSISSTSGGDSLTPGGTPIRQVLKIVGKAGAKIKLRVKVKYSVSGNSVEEQFDFAGFPETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIF0</accession>
    <name>G3AIF0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_49463</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374005.1">
        <property type="nucleotide sequence ID" value="XM_007373943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIF0"/>
    <dbReference type="GeneID" id="18871342"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49463"/>
    <dbReference type="eggNOG" id="ENOG502SB6R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_716042_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIF0"/>
    <dbReference type="OrthoDB" id="1744952at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="174"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="346"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="9"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="301"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="386" mass="42781" checksum="00DE79BA875BD18B" modified="2011-11-16" version="1">MTKPNSKAKPTEAKDAHDDGINDSLNEKTSRPKKEETSAKEQTKGGSGESSTDASSPSIPPLSNPTVASNTHMLPPITVKKNAGSTIPTNTQSEFNPYVTFSSKYPGGQQQPPPPLQIIQDTTHQVNSNETNYFFKSGRPKVRVDDYNIKKFSVAKSPLTSQSTPGSAPATRPHTCTFEGCTWSFERKSDLKRHLKSHAPPIYQCPYYRNDPTCHRNGGAFNRLDVLKRHLRLVHYVKDKHQMVPPAPGPMVGSTGGAMVLPTKKEDPGWCRACQKMFPNSKIFIDHCWDCAQHITPAEWKKAGDTGDTGRDQESDRTTQQQAYQALAARSNNLYELSRISTEEKEAASAFFTSQSESTEGESKRKTSAEEPTMKVEPAHKRTRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIL3</accession>
    <name>G3AIL3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Inositol-3-phosphate synthase</fullName>
            <ecNumber evidence="6">5.5.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Myo-inositol 1-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">INO1</name>
        <name evidence="18" type="ORF">SPAPADRAFT_59093</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucose 6-phosphate = 1D-myo-inositol 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:10716"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="5.5.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the myo-inositol 1-phosphate synthase family.</text>
    </comment>
    <dbReference type="EC" id="5.5.1.4" evidence="6"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373312.1">
        <property type="nucleotide sequence ID" value="XM_007373250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIL3"/>
    <dbReference type="FunCoup" id="G3AIL3">
        <property type="interactions" value="697"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIL3"/>
    <dbReference type="GeneID" id="18872768"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59093"/>
    <dbReference type="eggNOG" id="KOG0693">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021486_1_0_1"/>
    <dbReference type="InParanoid" id="G3AIL3"/>
    <dbReference type="OMA" id="CTRPRDW"/>
    <dbReference type="OrthoDB" id="2887at2759"/>
    <dbReference type="UniPathway" id="UPA00823">
        <property type="reaction ID" value="UER00787"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004512">
        <property type="term" value="F:inositol-3-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000334">
        <property type="entry name" value="Inositol-3-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000055">
        <property type="entry name" value="Putative myo-inositol-1-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002587">
        <property type="entry name" value="Myo-inos-1-P_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013021">
        <property type="entry name" value="Myo-inos-1-P_Synthase_GAPDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11510">
        <property type="entry name" value="MYO-INOSITOL-1 PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01658">
        <property type="entry name" value="Inos-1-P_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07994">
        <property type="entry name" value="NAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015578">
        <property type="entry name" value="Myoinos-ppht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0398">Inositol biosynthesis</keyword>
    <keyword evidence="13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myo-inositol-1-phosphate synthase GAPDH-like" evidence="17">
        <location>
            <begin position="143"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00070063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW33728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="348" mass="39387" checksum="EDCE3F72F1CD8CAC" modified="2011-11-16" version="1">MPSIYYPDFIALNQKDRADNIFNKTESGEVRTDNKWADVERIRKDIREFKAKNDLDKVIVLWTANTERYADVIANVNDTSDNLLEAIKSNHEEIAPSTVFAVASILEKVPYINGSPQNTFVPGVIELAEKYDSFIGGDDFKSGQTKIKSVLAQFLVDAGIKPLSIASYNHLGNNDGYNLSAPKQFRSKEISKQSVVDDMIESNEILYNKESGDKVDHCIVIKYVPAVGDSKVAMDEYYSELMLGGHNKISIHNVCEDSLLATPLIIDLVIMSEFLSRVQYKKVDEEQYHDFYSVLTLLSYWLKAPLSRPGFKPINGLNKQRQALENLLRLLVGLPVNNELRFEERLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIN7</accession>
    <name>G3AIN7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">NADH-ubiquinone oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_134311</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I NDUFA5 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374037.1">
        <property type="nucleotide sequence ID" value="XM_007373975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIN7"/>
    <dbReference type="STRING" id="619300.G3AIN7"/>
    <dbReference type="GeneID" id="18869802"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134311"/>
    <dbReference type="eggNOG" id="KOG3365">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099943_0_1_1"/>
    <dbReference type="InParanoid" id="G3AIN7"/>
    <dbReference type="OMA" id="ENQWKWP"/>
    <dbReference type="OrthoDB" id="286811at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006806">
        <property type="entry name" value="NDUFA5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12653:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12653">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 13 KD-B SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04716">
        <property type="entry name" value="ETC_C1_NDUFA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="15477" checksum="F36752A5EF3A5F3B" modified="2011-11-16" version="1">MRFTSALRQVQSVLVRSATGNPTGITGLYQHPNPRPALISLYKYTLDVLNNRYPKESVYRQSVEALTKNRLKIVEEEEIVENIEKRIGDGLIEEVLVQADDELKLAQEMATLKCWEPLEEKPLEDQWVYFGKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIQ0</accession>
    <name>G3AIQ0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_49509</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374050.1">
        <property type="nucleotide sequence ID" value="XM_007373988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIQ0"/>
    <dbReference type="STRING" id="619300.G3AIQ0"/>
    <dbReference type="GeneID" id="18871348"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49509"/>
    <dbReference type="eggNOG" id="ENOG502SP70">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_310497_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIQ0"/>
    <dbReference type="OMA" id="CIITRAG"/>
    <dbReference type="OrthoDB" id="1865897at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="647"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="656"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="996" mass="114270" checksum="244472002200F164" modified="2011-11-16" version="1">MIAKVLRVTQVIGKRRIRPNVYIRFISTRSDERSPFLFTPNVDQHPGINGSKLQEQYSQYYRNALTFKNPNYYYRDLEFEDMDDVEINSTPAEDFGVILDRNLNSVPVPGSVVEFIHPTTLEVKFGVVIRESQARFDESLNRLVVLMPDNEVVQISPMDVNFHCHSLIYGDFETIYENIMHTDIDDRLYVISAMHKFIHDSHIMKKLFEDKLDRLFFQFATSRIRALSFAHLIDATQFPEHMIDVIKESRWNQCALFMGLHLNLVNSCRWIVPKYIPANKGSNIFNEEHSNNYVSRPIYLVNTEDNWIAISKFLDDMKTENGFHNLNHEFSSFYKHQTSKLAVEGDKLNAALSTSTHRHFAHVIEMLKFMVIYPHDTLLTEIKKLDLFKNVETASDIYNILEELKIFKHGQASDVLVESGILGEVNKSKEVHEVLGSDNFRHLRGKGFSRDLVVYGLNLNYPSNSQTPTIGVSLEKILRDEHILRIHIPDPITFHSPTSKPMRKAFHPEYGFVSGMDHLINNQHWFPLFPNKGFPSWHDKTAKNVETSNFSTCMTVSFKYNPLADDPFQDLQSLVSVRMDNILSCNIKKLDDKELEKCLAGVRIAPDVVSWLEGTSERTSNLTLNDQETIRGIYSIMQRFFKRRSIHNASPPDPLQTPSSIDSSNLSHGLVTTVKNDFSFMLEEESAEQVKKDTSVTAYKVTNHWPNTHESHFLINELRVLVGTLVSEYCIDKNIPILRRAQHLNTGDFPEDKVIVPHRTVLQKSYDATSYSYMLYSKNSKGYMTPQNVLVSKSFLAPESITFRDVEDKENIVLGLDNGWANIVDVYSKGEAVVNQLQILMSLQYTFQRAAIRRGASLIAVNEKFSNLRRLGYETNGAFPERVLAGQIENIINGDNDSKYVMQTIKKYWTLRCLQQDVRKVQEFECMITKVGVSMMPGVILSKGYCVELDIEVDVLTDVPNQYVGMPISCNQVVWMDPVGGACLLKDKKLVIDDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ61</accession>
    <name>G3AJ61_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_54102</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373402.1">
        <property type="nucleotide sequence ID" value="XM_007373340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ61"/>
    <dbReference type="FunCoup" id="G3AJ61">
        <property type="interactions" value="327"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ61"/>
    <dbReference type="GeneID" id="18871836"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54102"/>
    <dbReference type="eggNOG" id="KOG0596">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019372_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ61"/>
    <dbReference type="OMA" id="ERHEHND"/>
    <dbReference type="OrthoDB" id="20524at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043515">
        <property type="term" value="F:kinetochore binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033316">
        <property type="term" value="P:meiotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051988">
        <property type="term" value="P:regulation of attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031134">
        <property type="term" value="P:sister chromatid biorientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030474">
        <property type="term" value="P:spindle pole body duplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14131">
        <property type="entry name" value="PKc_Mps1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000131">
        <property type="entry name" value="Dual specificity protein kinase TTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027084">
        <property type="entry name" value="Mps1_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974:SF21">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE TTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974">
        <property type="entry name" value="MIXED LINEAGE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="254"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="56"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="580" mass="65840" checksum="9AD5F2419B234908" modified="2011-11-16" version="1">MNHTPPATFLKREFTDDHHNNTSSTSSYKITGVYELDFPENKRAATLRSKLSAHIGSPAQTRTGDASFSTCSSNSTNVSSKSTAVSTVRSLPRKRKSRSFGLNLGLPGRASDQPPPSQQQQQHQESDQERHEHNDRLSVKEETLKQETSIKHEKIGSPILLKHEHVGSPAEQENFRYDFEERKKFMFPNVLTSDTIENKENKEFAFKDISSRPALAEIPVNIDTFRKPKLPKPIHSTESHISPKRIISINGKQYEKLELLGRGGTSKVYRVKSLSNNQIYAIKKVSNGQYDEISLAGFKGEIDLLLKLKHSDRVVRLIDHAITEGSIYLVMERGEIDLAQVLNNKLRSQTPFDLDFIRFHSLEMLRCVRAVHDAGIVHSDLKPANFLIVKGILKIIDFGIANAVPDHTVNVYRESQIGTPNYMAPEALTEVNHSMPRNTWRVGKPSDIWSIGCIIYQLIYGRPPYAGYSGTQRMMAIINPQVKIQYPATGVNGGVVPESAIELMKMCLKREPHERWTVEECLRCDFLRPKVVNRKLVQDMVHEIMDYGHNRRINNEAIGSDEYDDMVELVMEKITHLNYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ70</accession>
    <name>G3AJ70_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59185</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373411.1">
        <property type="nucleotide sequence ID" value="XM_007373349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ70"/>
    <dbReference type="STRING" id="619300.G3AJ70"/>
    <dbReference type="GeneID" id="18872812"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59185"/>
    <dbReference type="eggNOG" id="ENOG502SDEG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141190_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ70"/>
    <dbReference type="OMA" id="SWANIKI"/>
    <dbReference type="OrthoDB" id="4023585at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="158" mass="16914" checksum="B229FB19B7AE1024" modified="2011-11-16" version="1">MLRTTIIKSTPIFYTQSCSYSVLQQVKGGLSWANIKIGKAAAKDIEIAERAAQATVHSAETAGNVAKTAASTLNKTTGKVLAGGIEVAEEAVHEIQHAEEVLNKGGGKVLAEGIDLAQTWKHHHDAVARVRQNKKGYTTLQEKGAKVETEQNRPDDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJL0</accession>
    <name>G3AJL0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_65736</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374199.1">
        <property type="nucleotide sequence ID" value="XM_007374137.1"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJL0"/>
    <dbReference type="GeneID" id="18875059"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65736"/>
    <dbReference type="eggNOG" id="ENOG502SBIP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_236469_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJL0"/>
    <dbReference type="OMA" id="HMPTTYG"/>
    <dbReference type="OrthoDB" id="4026618at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="7">
        <location>
            <begin position="2"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="192"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="279"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="875"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1015"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1277"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1313"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1515"/>
            <end position="1540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="197"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="284"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="311"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="888"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1277"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1515"/>
            <end position="1539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1869" mass="205016" checksum="128355C933A3CF4B" modified="2011-11-16" version="1">MAIPGNGCIFLEEGSLVVLNTLFKAPHQKFIMGDGATIQITSLFPQTYQVRKFQQGNKISLGGKLSNDGVSYDSQTGRLTLKTSIFTYYFIIGRGYNPSLFLILPDGQSLTYQDVAPIDPYQEEEFTAEVIVSTDSQSQWYTSTYTFSDEELTTTEYLETTDEELNSLEISTYVDSEFLSLESLTSNSIEDEIKESSGLTEGDYSSTMDNMSESIPKVNTPSELVSMTDEGDLQSSDEIISAQPTDSELPLTSEILDFLSSSDFESFIIIESIESYGSDRLISGDSNDTTGGSEPSTDDVEMFESLSDNSDSSEESEEPIQDSTSVQVSHEESTNTEDYIEQSTESNSNSITFDQIVLDTTGFSESSFSLLDESTATESPSVEDSFVYTNTKESQGSHSFEITEESINSEYIHIIDSQSLESLDTTSMEEIIIEDTNLIGSTSQSDDPFAETSISEVVIYSSDRSYEEIQLSSDETIVIELTESESEENIHDSISYESIALETSIDTSNHEYFETTKQSSETIDHSPETTNSETFTLVENTKLFESTDMVSSDEFTTQNESISQLFSSETHEITATSDDVSEDVLTEQTITPALPSTSSNQEYQTENMETPQEGMSYLSTINIDYQESQSTEKEILPGESITRTEHIFFSIDSTEDTEFGLSSGQVVPIELTLSVETKGILEYTTGKYSTEETEQEYYKSEEILSIDEISTEDFKSTDDSEGLDLETSSNVVPAESINIETESLGKSIVISSEISLELIESEILTFESTSDIYKEVTSISDSLDIRISSDFNFETIPHNEPTSMSTEEDYQPISSDIISEPYAFTSLSDATILITEELPHSSSIGTGEYISHYSEEIESLAISEEVNTNETIDREHLSLPSSDEGWVDSTQESYPDNYTTESESSNIDPKIQSISSKDEYQVVTSVVDAASTELDPEQSEDKFQLDSTAEGGIEFISEESFDKEFTDDSTIPIVTPLSESIVLLESSIVTVATVESIIPESFPSELESIEYIPKSTISEEPFPELSSSDTGGSDKYLTKETQSRQATESTAEIFKSETEKPVPIHISSNDSGEEGILTNEVISEESTYDVIVDASKWLTMDENTTSMEQWYEPGTEETYPSQSITDGDIGPSTEQLFPWESISEQESLSTKQVLPAESSVIEQIEFLETSSGEMTGEIPSADVTISLSPTEEEIQVRSTIDMNVGSVTPLTSETLRGGPSTAINEQGFEFSSKVIHPPESSYIGEGVHSETEDIVFLESSVDVQVEPITTDELISREYSGSEESSSFRTEEIDSGPNSTDREMVIEPTRVSVVSDESTNDGESLGSRTEEIDTSVSTDRGADTIHSQSKEVISEWSTDVQDSVVNTGTYDHLSSAEPFGSSDLLESQSVLTITPNSQGEILESIGPYTNPEKPTSTEIHPNEYSSDEGGYKFESTDVVAGQSTEQGEQLLQSSTYMTLLPEYTDEPGGLHSNVKSTHATASEIEPVSGTEFLVGESTNYSGQTWFTPVELTISNTEKPGSRDISTQEPVPGNIPSTSDTRFNKESLVSPASDKLLSQESFLTSDYGLVSDSTDIVGTASISSRDITNSGKGSTDYKVSFTNTDVPATDSTGSVNLHSATTVIKQGESTHSENLYSDSSKTIVKPTNDLSTSCKSCGENTASLSTIFHSDSVELIIPTKSQSDQSEIFSTFYSKYTTEYTNEMGQKTTAEVVISSNSKGNWFKTTRELVSTKVYSTSTNTRGEVSINEVIVSTDSKGNKHTHTTNIPVSIISRYDNDSIKDSEGVKTIPPDGRPMLSLQSKSTTAFTGEDENIDIIDTVETIDRKSRDQSTSSQNSAGYVLIDYADSSRIQKVKTSIYLLCGLACFVLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJR9</accession>
    <name>G3AJR9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ML-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_135246</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transient receptor potential (TRP) ion channel family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373554.1">
        <property type="nucleotide sequence ID" value="XM_007373492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJR9"/>
    <dbReference type="FunCoup" id="G3AJR9">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJR9"/>
    <dbReference type="GeneID" id="18869877"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135246"/>
    <dbReference type="eggNOG" id="ENOG502QSVZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010226_0_1_1"/>
    <dbReference type="InParanoid" id="G3AJR9"/>
    <dbReference type="OMA" id="FQAQAFI"/>
    <dbReference type="OrthoDB" id="5212126at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010308">
        <property type="entry name" value="TRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040241">
        <property type="entry name" value="TRP_Flc/Pkd2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032800">
        <property type="entry name" value="TRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145:SF2">
        <property type="entry name" value="FLAVIN CARRIER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_7G01610)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06011">
        <property type="entry name" value="TRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14558">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01320">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442370" description="ML-like domain-containing protein" evidence="9">
        <location>
            <begin position="19"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="364"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="418"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="445"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="507"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="533"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="564"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="598"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="ML-like" evidence="10">
        <location>
            <begin position="20"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="697"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="727"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="749"/>
            <end position="792"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="792" mass="88259" checksum="A6F0DDA33D2B21FD" modified="2011-11-16" version="1">MRLLQILIAFWLSQTVMAANHITSTSLLTCMDNSQFTASLFQIYFFPDNNTFTFNVNAISSIDNKNVTANVNLIAYGLNILQRNISLCSLDYQDQFNSKNNPLCPLTSGHLDLSSSYEVSSSVTKDIPGVAYTIPDLDARVRVLVYDSSNNNQLACVEAVLSNGKTVQTKYAAWPIAAVAGLGVITSGVVSVIGHSSTAAHIASNSMSLFVYFQSLVITAMMAVARVPPIAAAWAQNFVWSMGIVKLGFVQDIANWYVQSTGGTPSDILRSNYISVSVQKKLRMVKRAAMGVLNQIDFIPNMGKVNFVKRADISLDQQTFGLGDKLDPKLYTIDEKDKDISGKILVLRGIQRVAYLTGIEITDLYMTAIIFLFFFCFVMIVCLMLFKAVIEILIRSKIMNEGKFNEYRQQWASIIKGTIYRLMVLSLPQVALLCLWQFVERDSVGTIVVAVFLLVICVVLLFQAAVRVWIKGRESVREFKNPAYLLFGDGKFLNRFGFLYVQFRADCYYFVLVTLIYIFLKSLFVAVLQHQGMAQSVIVFAIELVYLVLLCWIRPFMDKRTNAFNITIGVINTINALFFMFFSNIFRQPQVVSSVMAVVYFVLNAVFALFLLLFTIITCVLALLYKNPDTRYQPMKDDRVSFLPRFGKTDDQVSPQQDMELMELGATAMKGHEHGGPETSPIGAKKYEEDESVYGSEAYHPVNTNSSSNIYEDPSKRDSFMFDSDPIQPNSTIVGNPYNAAQSRGGFSYGSTPNRGPSGASSSYPSTTGGPQNPFNQSTGYGDNSGSRMDYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJS0</accession>
    <name>G3AJS0_SPAPN</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59381</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007373493.1"/>
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    <dbReference type="AlphaFoldDB" id="G3AJS0"/>
    <dbReference type="GeneID" id="18872889"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59381"/>
    <dbReference type="HOGENOM" id="CLU_2198637_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJS0"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="108" mass="11656" checksum="0BCBEB600BEAC31E" modified="2011-11-16" version="1">MTTVAIAQVTIHTVVPIAKAQQPDPIVAHVIGIHAAATVAPAIIIIPAKILSETYFRKLRFSRASAPIVLALIIVLIIYVNDAAMIVNKITMMKTLDSNICTLFIIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJT8</accession>
    <name>G3AJT8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_59398</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007373511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJT8"/>
    <dbReference type="GeneID" id="18872898"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59398"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056878_1_0_1"/>
    <dbReference type="InParanoid" id="G3AJT8"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF70">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="271"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="299"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="175"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="175"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="189"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="372"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW33989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="405" mass="44544" checksum="C6FB2A0FE9648274" modified="2011-11-16" version="1" fragment="single">MFNYNSYYNSNNGMPQTDPHKQPPQPLQPSQSHQQQMPNVQYIQTQQNQSILQGQKQQAPQLPQQNQALPSIFASLPQQESVSANTNISSSAVPAQFMAYSASVPQLPPTPFSYDYSSNQYNGANSANGYSQQPRPYSALPIPQAGGYSAYYQYNNAAVNGSQAALPVLPSNSQRYPLSPLSGQSDNNSTVGKSSATSSKGSSTSPPDIDYSSLVSYTISRSIRRKRRKKATDATPLNPVESFPCEVCNKVFQKHYNLKSHMKTHSTEKPFKCSHCFKTFARSHDKKRHELLHNGIKNFKCEGYLQDGVTKWGCGKKFARSDALSRHFRTETGWLCIRPLMDEAKRLEDAGISSSISVPVAAGAIIPSSSSVSTSIASTNSTNSRSPNGEEYYDNTSLIKKLIQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK86</accession>
    <name>G3AK86_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vacuolar membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_60885</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375060.1">
        <property type="nucleotide sequence ID" value="XM_007374998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK86"/>
    <dbReference type="FunCoup" id="G3AK86">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK86"/>
    <dbReference type="GeneID" id="18873616"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60885"/>
    <dbReference type="eggNOG" id="ENOG502S1HE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040321_2_2_1"/>
    <dbReference type="InParanoid" id="G3AK86"/>
    <dbReference type="OMA" id="LNCFQYF"/>
    <dbReference type="OrthoDB" id="431202at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022127">
        <property type="entry name" value="STIMATE/YPL162C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31735">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YPL162C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31735:SF1">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YPL162C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12400">
        <property type="entry name" value="STIMATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
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            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="37464" checksum="E79FAE7CAB829C50" modified="2011-11-16" version="1">MLGCSEIRSIIPMALLSLILPLSFNSTGRTGGNTGNTDDDGKCELIGTFSLFTQAFLGLLCISSLMVKRFYEYPIRRTWPVWVFDVAKQLIGAFGVHMFNVILSMAKTSDEEDFRRILQTDDEETQDPCDWYFLNIVFDCTIGVYILYLVFKLMNRICKKYFRITQIESGEYGPDPNNPSTRAFFKQLGVYFTSLMITKFILYGIVECFEKQLLWITSHILLVWLDEYPDEFEIFIVMFVVPIFMNCLQLILIDNFIRNQLWITTNKRIKLEYPNFRDDTESVLRQEIAEILQEEARRESQGETTPIGRESRTSPKTYGTIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKF2</accession>
    <name>G3AKF2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_60251</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374424.1">
        <property type="nucleotide sequence ID" value="XM_007374362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKF2"/>
    <dbReference type="GeneID" id="18873295"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60251"/>
    <dbReference type="eggNOG" id="ENOG502S1EZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056292_1_0_1"/>
    <dbReference type="InParanoid" id="G3AKF2"/>
    <dbReference type="OMA" id="IIPDREH"/>
    <dbReference type="OrthoDB" id="421671at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025638">
        <property type="entry name" value="DUF4336"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33835:SF1">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33835">
        <property type="entry name" value="YALI0C07656P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14234">
        <property type="entry name" value="DUF4336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="30249" checksum="0EFCB0AC55060F4D" modified="2011-11-16" version="1">MGYPDNFKIVTKQLSDNLLLASTSFTRVNKFNFGARMAVFKTNQDLIVWSPLPYNDQVVSTLKEFSGDKFNITHVIVPDREHNLAAKSYKDKFPTAKIIAMENITSFKPDYLFTKANGNKVLKGEELKQIVKDDVIVEKFEFVYLPFHENQELVLFDKQTKTVFEADLLFNLGEPGTTSGKVVLEQFSPELGFKPGFNPHGGWSFPTRYLQPYSKVGRFLFRKIVNPAKSAPGLNAIYSWDFNTIVMCHGNIITKGAKDAFKHVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKU1</accession>
    <name>G3AKU1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_50498</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375158.1">
        <property type="nucleotide sequence ID" value="XM_007375096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKU1"/>
    <dbReference type="STRING" id="619300.G3AKU1"/>
    <dbReference type="GeneID" id="18871448"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50498"/>
    <dbReference type="eggNOG" id="ENOG502RMH2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097629_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKU1"/>
    <dbReference type="OMA" id="RRYNICN"/>
    <dbReference type="OrthoDB" id="761538at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01309">
        <property type="entry name" value="UPF0057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="72"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="125"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="170" mass="19234" checksum="AA479A8968B81765" modified="2011-11-16" version="1">MANTQPQQEQFILIIAALVLPPLAIFLAKKYSIWNKEFLVSVLLTMFGHIPGAIFTLYYLWYIEFPQSRQGGYNRLPDDDESRLGQQQEGFYEDHDHHQESRVGAQEEGFYEPEQLKQPQPQQQGNAGSSSSRPQNDALAGDLPAYEDIVQPLQAPAVDVKGSSDNKIQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL08</accession>
    <name>G3AL08_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial import inner membrane translocase subunit TIM17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_60369</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Mitochondrion inner membrane</location>
            <topology evidence="2 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the Tim17/Tim22/Tim23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374566.1">
        <property type="nucleotide sequence ID" value="XM_007374504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL08"/>
    <dbReference type="FunCoup" id="G3AL08">
        <property type="interactions" value="1166"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL08"/>
    <dbReference type="GeneID" id="18873370"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60369"/>
    <dbReference type="eggNOG" id="KOG1652">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087811_3_0_1"/>
    <dbReference type="InParanoid" id="G3AL08"/>
    <dbReference type="OMA" id="FDCTFQY"/>
    <dbReference type="OrthoDB" id="2261329at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005678">
        <property type="entry name" value="Tim17"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00980">
        <property type="entry name" value="3a0801so1tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10485:SF0">
        <property type="entry name" value="AT05822P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10485">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM-17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW33051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="154" mass="16349" checksum="AD6A7B88E64FE204" modified="2011-11-16" version="1">MSADHTRDPCPIVILNDFGGAFAMGAIGGCVWHGIKGFRNSPYGERSYGALSAIKARAPVVGGNFGVWGGLFSTFDCAVKAVRKREDPWNAVIAGFFVGGALAIRGGWKHTRNSAITCACLLGVFEGVGMMMQRLQAQPTMAPQYPEEPVQLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL12</accession>
    <name>G3AL12_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamine amidotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_49980</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374571.1">
        <property type="nucleotide sequence ID" value="XM_007374509.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL12"/>
    <dbReference type="FunCoup" id="G3AL12">
        <property type="interactions" value="105"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL12"/>
    <dbReference type="GeneID" id="18871393"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49980"/>
    <dbReference type="eggNOG" id="KOG3179">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054974_0_2_1"/>
    <dbReference type="InParanoid" id="G3AL12"/>
    <dbReference type="OMA" id="PWIQTLK"/>
    <dbReference type="OrthoDB" id="3993678at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01741">
        <property type="entry name" value="GATase1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044992">
        <property type="entry name" value="ChyE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695:SF5">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="1">
        <location>
            <begin position="113"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="264" mass="29497" checksum="F2F0BA50B70C3194" modified="2011-11-16" version="1">MTVENQDNVSHVAVLVLDTPIPNITEKYGDFGDNTKDLLSKHTTTKIKKYYLAYSTSPEEYLTQLQSTYKELEAGIKQGYITGLVLTGSRSDAFGEEPWIKLLDEFIVNVIIPSKVQTAGICFGHQILCKNLGAKIGRNEIGWEAGTTPIEVNPELKKNSPFSGLSKFNMVEFHQDIVYELPPGCVSIGSTDKCNIQGVLSPNLLTFQGHPEFTTELALDLLKYKFEHGLLTKQEYEHYKSQTVTFPNQGTEIGEIIAKFLNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL43</accession>
    <name>G3AL43_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Small nuclear ribonucleoprotein Sm D2</fullName>
            <shortName evidence="12">Sm-D2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 12">snRNP core protein D2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_60395</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the snRNP core protein family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374601.1">
        <property type="nucleotide sequence ID" value="XM_007374539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL43"/>
    <dbReference type="FunCoup" id="G3AL43">
        <property type="interactions" value="1211"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL43"/>
    <dbReference type="GeneID" id="18873385"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60395"/>
    <dbReference type="eggNOG" id="KOG3459">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_2_1_1"/>
    <dbReference type="InParanoid" id="G3AL43"/>
    <dbReference type="OMA" id="DVKEMWT"/>
    <dbReference type="OrthoDB" id="437526at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036261">
        <property type="term" value="P:7-methylguanosine cap hypermethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01720">
        <property type="entry name" value="Sm_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000018">
        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027248">
        <property type="entry name" value="Sm_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12777">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="13">
        <location>
            <begin position="30"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW33086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="112" mass="12983" checksum="B258809EA2600507" modified="2011-11-16" version="1">MSEYIDRPRSELTELELQKLEEFEFTHGPMSLLQNAVNNNTPIVISCRNNHKLIAKVKAFDRHCNLVLENVKELWTETVKNNKGKKIKSTPKERFVSKMFLRGDSVIIIVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL86</accession>
    <name>G3AL86_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nop domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_150803</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRP31 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374644.1">
        <property type="nucleotide sequence ID" value="XM_007374582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL86"/>
    <dbReference type="FunCoup" id="G3AL86">
        <property type="interactions" value="1211"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL86"/>
    <dbReference type="GeneID" id="18870882"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_150803"/>
    <dbReference type="eggNOG" id="KOG2574">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026337_3_1_1"/>
    <dbReference type="InParanoid" id="G3AL86"/>
    <dbReference type="OMA" id="EMRRSAN"/>
    <dbReference type="OrthoDB" id="4771285at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027105">
        <property type="entry name" value="Prp31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019175">
        <property type="entry name" value="Prp31_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13904">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR PRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13904:SF0">
        <property type="entry name" value="U4_U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09785">
        <property type="entry name" value="Prp31_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Nop" evidence="10">
        <location>
            <begin position="247"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="367"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="481"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="383"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="484"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="499"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="519"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="537" mass="60539" checksum="5F1A4283DE0186F3" modified="2011-11-16" version="1">MDEDYEKELLADLQGLSDGEELDKDEDLVLNNEQGSTTDSFEQRLHDVIVANSVSNSFQSILNNPNIEEIDDLTSLSRIYPLIPELTAKIEQYANDEETDYLELLSSLSTGMFDQSNEYKFILTVNELSTIINNEISVFVTLIKMQYKIVFPELESLIMNPIDYVRLILIFKQDLKNIKSYELQMKNIISNEKVLVVIMAALHQVSKQFVLSEDDMNKILSCATLVLELEDILHKLSTFISGKLSKFAPNVSAIIGPIATSQLLIATGSLKQLAQTPSCNLPSLGVRDLSSTQKNAPRNIRQTGYLYHSDLVRYLPPDIIRSVMRIVSGKVILAARIDLSKSNPNGESGQKFLEEINVKIEKLLTPPEQTPDKALPVPVEQKSKKRGGKRFRKMKERFQMSELRSAQNKMEFGKEEDTVMDGFGEEIGLGMTKSGGSGRIGQIKVNTNTNARMSKAMIQRLQKQQQDTRQLKQSMFDDDLDSLILNNPSSNKQVGTSKPGEKPKLDLKNKWLGGISKKRLNDSSDDENKDRNKQRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALC2</accession>
    <name>G3ALC2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cyclin-dependent kinase 8</fullName>
            <ecNumber evidence="4">2.7.11.22</ecNumber>
            <ecNumber evidence="3">2.7.11.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">SPAPADRAFT_60474</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="4"/>
    <dbReference type="EC" id="2.7.11.23" evidence="3"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374680.1">
        <property type="nucleotide sequence ID" value="XM_007374618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALC2"/>
    <dbReference type="STRING" id="619300.G3ALC2"/>
    <dbReference type="GeneID" id="18873431"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60474"/>
    <dbReference type="eggNOG" id="KOG0666">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="InParanoid" id="G3ALC2"/>
    <dbReference type="OMA" id="EVRIDMN"/>
    <dbReference type="OrthoDB" id="6284126at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990508">
        <property type="term" value="C:CKM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060258">
        <property type="term" value="P:negative regulation of filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031648">
        <property type="term" value="P:protein destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000408">
        <property type="entry name" value="Serine/threonine-protein kinase SSN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF495">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0010">Activator</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="252"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="252"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="23">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="EGW33165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="280" mass="31212" checksum="5EE5EF0DDB33D53A" modified="2011-11-16" version="1" fragment="single">ILNGVTFLHKNWIFHRDLKPANIMVTSQGVVKIGDLGLARKFNNPLQSLYTGDKVVVTIWYRAPELLLGTRHYTPAVDLWAVGCILAELLSLRPIFKGEEVRIDMNNKKSVPFQKNQFQKIIDILGMPNTKTWPSLNKYPEFATFSSQLTQSPNSGQSNLVSWYKMIGGSNKQCLELLKGLLEYDPEIRLTAENALIHRYFMELPKVNDNAFEGLNFRYPNRRIYTDDNDIVSNQPAVSAAAVAAAAAAANQGGNANAGNSGYKRVGDDVNASHSRKKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALI4</accession>
    <name>G3ALI4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LicD/FKTN/FKRP nucleotidyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_60563</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374742.1">
        <property type="nucleotide sequence ID" value="XM_007374680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALI4"/>
    <dbReference type="GeneID" id="18873467"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60563"/>
    <dbReference type="eggNOG" id="ENOG502QREF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_659170_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALI4"/>
    <dbReference type="OrthoDB" id="444255at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009644">
        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF28">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE TRANSFERASE FKTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="6">
        <location>
            <begin position="2"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="6">
        <location>
            <begin position="107"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="343"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="348"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="382"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="417" mass="47693" checksum="D009E276C5644AC1" modified="2011-11-16" version="1">MQFPWDGDVDVQMPIADLHKLAQLFNQSVVVDFGNDMDNLRYGRFFVDVTSSISHRDKGNGRNNIDARFIDMDSGLYVDITGLAISETKAPDRYDGGLRNSKLERSRKDVTEAQRNAHLKVYNCRNKHFTRLDELSPLKLTLVEGEFAYVPEQFTTILVDEYQTKSLSVRDYRQMAFMSNLRNWVDRDKVLDLASHKDSDRKKRLAKIKVTPDLNIPDSVQLLMDNEPVLQEYLTTKELTEIHANELNAILSGISPSSLFFDDETNSLLDLRSTIRPDVFTWETIQKEYNYEQAMAEFNEKCQDSDKILSIKEGAEQLKKTSNEMEKSAKAKEKIIEKAVEDVKKETAQQAPPQPAQAISNQAPPKQQAQHVQPVKQAQLANQVQEPEPQPNQDVNEEPNQPKLLQQAQAPIERDML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALK5</accession>
    <name>G3ALK5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">U1 small nuclear ribonucleoprotein component SNU71</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_50145</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the U1 snRNP particle, which recognizes and binds the 5'-splice site of pre-mRNA. Together with other non-snRNP factors, U1 snRNP forms the spliceosomal commitment complex, that targets pre-mRNA to the splicing pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNU71 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374763.1">
        <property type="nucleotide sequence ID" value="XM_007374701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALK5"/>
    <dbReference type="FunCoup" id="G3ALK5">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALK5"/>
    <dbReference type="GeneID" id="18871410"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50145"/>
    <dbReference type="eggNOG" id="ENOG502S6GF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_478950_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALK5"/>
    <dbReference type="OMA" id="NTITRHY"/>
    <dbReference type="OrthoDB" id="6275295at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1390.10">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002483">
        <property type="entry name" value="PWI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036483">
        <property type="entry name" value="PWI_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01480">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00311">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101233">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="PWI" evidence="8">
        <location>
            <begin position="457"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="417"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="422"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="533" mass="61061" checksum="60EE1EE7558C5F8E" modified="2011-11-16" version="1">MTRGIITVSPYSIDQRSNILSLLKNPPPAAVNPKLALTVPTLSSLDTSSLINLNTTRIHAQDAHRSGASLNATNTDVASSTIDQTSLSEEKLAHYVPISSLQLASLKDQLNTIIIKNIANLPSSSLSKRLSAIERVLNMCMRIKSVEYTWSIINHEYLLDLNLVYVRFAHTRDVSWFLTSFDVSALFGGEVITNEQFVLDESAAKSEVPDSIKTEFQLQTNNTTKKSGAVDESQEILNYYKSQYKVDKSELVDIPNSMKEATILNILKFRSRVLIMEKENRKRELEKERVKTREKLKSLFEEKQIDTTAIANEEHKKEAVDRDEFEDMDEDEYKEYLEFKADEQLTKQYQDAIAKISIKESQRSQLLSQLEQLTNYEDNLIDNKLKHIDDIKNQDNHLVNLYNSNYSHYLKLRSHKRTLEQANDDNDRDEELEELKRQKPPQEEAVVEPPSVEQEGGIQVNVSQLQPKVIELVKEYLGINDDFLIEVINEHLSTHGLSNKTNLVEELTQVLDEDAISLVDDLWEYVKTTNQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALM5</accession>
    <name>G3ALM5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60605</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374784.1">
        <property type="nucleotide sequence ID" value="XM_007374722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALM5"/>
    <dbReference type="GeneID" id="18873487"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60605"/>
    <dbReference type="eggNOG" id="ENOG502SF3E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1180851_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALM5"/>
    <dbReference type="OrthoDB" id="4089867at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="235" mass="27057" checksum="CC9E79BDCD00F381" modified="2011-11-16" version="1">MATLSQIETVTNNNGQVQEIVRRNSINDMMNTLANLDLLNGNSFIKENKKEPVPEEHEIKTSSKTPLHCNTTPVIKEYWTDKDTSMDQDLLDEINQLPEDFEFEDHESKRPSSSPAFFRSNSYNKKPSKSVMSNQYQSNKIETTNKTVTFYRNSYSAPSLEIKKADSISRTPSARSYNSCTSVREEENSDEEDVYDGPKMRFYSQFMINDLHSNDSFSRQNNLKTISEGESPFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALN9</accession>
    <name>G3ALN9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_50181</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374798.1">
        <property type="nucleotide sequence ID" value="XM_007374736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALN9"/>
    <dbReference type="STRING" id="619300.G3ALN9"/>
    <dbReference type="GeneID" id="18871414"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50181"/>
    <dbReference type="eggNOG" id="ENOG502S9MS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_654106_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALN9"/>
    <dbReference type="OMA" id="DENNMAR"/>
    <dbReference type="OrthoDB" id="4090114at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033768">
        <property type="term" value="C:SUMO-targeted ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038886">
        <property type="entry name" value="E3_SLX5/Rfp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28042">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE COMPLEX SLX5-SLX8 SUBUNIT SLX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28042:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE COMPLEX SLX5-SLX8 SUBUNIT SLX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="162"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW33283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="420" mass="47967" checksum="3B1694841CFF1EA9" modified="2011-11-16" version="1">MDPTNNIPNISVPSRPRKHSLPPHESTSSNPPRHNSIVEIVSDEEPEIIDLDNEPEPDSSFNNEVEIVSVNTENPTPITENISSVNSLFVPEETGDADLQDDVEITNVRRIGNNHHNHHTVHTPFGQFRVFMPDRRPVQQHEPIVVHPPQRATLRRLAHQPLESSRRAAQQRMQQSRLARDSRRHIQARPPGPPLISRILNFGRPFHILPFRNHPDPEEIAYFQNMMDAMEGIGSGGFESNAVEESILRRIERDNERDLNERLQRETSYNNKALQEKKKLAANHNEQTRHVTTIKSDENLVCELCGIVLGEGIPEDFAADPAYNEHFDKYTGQYQVQAPWFCIYPMTQVDKELSKRIFVAKCGHTFCGRCVKNIGNRPRRTKATRSGLTILNPSIFAPSKCPETKCMKKFTAKGFTEVYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALP9</accession>
    <name>G3ALP9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Terpene cyclase/mutase family member</fullName>
            <ecNumber evidence="14">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_55185</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(S)-2,3-epoxysqualene = lanosterol</text>
            <dbReference type="Rhea" id="RHEA:14621"/>
            <dbReference type="ChEBI" id="CHEBI:15441"/>
            <dbReference type="ChEBI" id="CHEBI:16521"/>
            <dbReference type="EC" id="5.4.99.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:14622"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="13">Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the terpene cyclase/mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="14"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374807.1">
        <property type="nucleotide sequence ID" value="XM_007374745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALP9"/>
    <dbReference type="FunCoup" id="G3ALP9">
        <property type="interactions" value="129"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALP9"/>
    <dbReference type="GeneID" id="18871915"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55185"/>
    <dbReference type="eggNOG" id="KOG0497">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009074_2_1_1"/>
    <dbReference type="InParanoid" id="G3ALP9"/>
    <dbReference type="OMA" id="CWARQTI"/>
    <dbReference type="OrthoDB" id="21502at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000250">
        <property type="term" value="F:lanosterol synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016104">
        <property type="term" value="P:triterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02892">
        <property type="entry name" value="SQCY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000003">
        <property type="entry name" value="Terpene cyclase/mutase family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000027">
        <property type="entry name" value="Terpene cyclase/mutase family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.120.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032696">
        <property type="entry name" value="SQ_cyclase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032697">
        <property type="entry name" value="SQ_cyclase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018333">
        <property type="entry name" value="Squalene_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002365">
        <property type="entry name" value="Terpene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01787">
        <property type="entry name" value="squalene_cyclas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764:SF20">
        <property type="entry name" value="LANOSTEROL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764">
        <property type="entry name" value="TERPENE CYCLASE/MUTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13243">
        <property type="entry name" value="SQHop_cyclase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13249">
        <property type="entry name" value="SQHop_cyclase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01016">
        <property type="entry name" value="Prenyltransferase_Like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01074">
        <property type="entry name" value="TERPENE_SYNTHASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0752">Steroid biosynthesis</keyword>
    <feature type="domain" description="Squalene cyclase N-terminal" evidence="16">
        <location>
            <begin position="78"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Squalene cyclase C-terminal" evidence="15">
        <location>
            <begin position="384"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF13249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW33292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="726" mass="83424" checksum="369C9A83B921A0E0" modified="2011-11-16" version="1">MYYSEEIGLPKTDPTRWRLRSSPLGSETWHYITDDEELAKEPQSTFVQWLLESPDFPAPEPSDIKTPGEAMEKGADFLRLLQLDNGILPCQYKGPMFMTIGYLAANYFSGTPIPDPYRHEMIRYIVNTAHPVDGGWGLHSIDKSTCFGTTMNYVCLRLLGMDPDHIVMQRARRTLHKLGGALGNPHWGKCWLAVLNLYEWDGVNPAPPELWTLPYSLIIHPGRWWVHTRAIYLPLGYVVANRVKCTLDPLLEQIRNEIYLPKQLPYESIKFSKYRNNVCGVDLYYPTTKLLDFANTVLCQWEKLRPNWILNLVNNRVYDLILKEYYNTEYLCIAPVSFAFNMVVTCHREGNESANFKKLTDKMNDVLFHGPQGMTVMGTNGVQVWDVAFMVQYFFVSGLVDNPRYHDMIRKGYMFLVRSQFTEDCVEGSFRDKRKGAWPFSTKEQGYTVSDCTAEAMKAIIMVKNHSAFSDIHDEITDEKLYDAVEVLLNIQNTESYEFGSFSAYEKIRSTLLLEKLNPAQVFNNIMVEYPYVECTDSSVLGLTYFHKYYPNYKSSLIESTIENAINYIKKAQDTDGSWYGSWGICYTYAAMFALEAFNAVGLDYETSRNVQKGCDFLVSKQLSDGGWSESMKACETHTYVNGPKSLVVQTAWAVIGLLLANYPDERPIKKGIKFLMDRQKPTGEWEFEDIEGVFNHSCAIEYPSYRFLFPIKALGLYKKNYGDII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALS8</accession>
    <name>G3ALS8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Agglutinin-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_137089</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374202.1">
        <property type="nucleotide sequence ID" value="XM_007374140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALS8"/>
    <dbReference type="FunCoup" id="G3ALS8">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="GeneID" id="18870013"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137089"/>
    <dbReference type="HOGENOM" id="CLU_031316_2_0_1"/>
    <dbReference type="InParanoid" id="G3ALS8"/>
    <dbReference type="OrthoDB" id="3981162at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903561">
        <property type="term" value="C:extracellular vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030446">
        <property type="term" value="C:hyphal cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030445">
        <property type="term" value="C:yeast-form cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044403">
        <property type="term" value="P:biological process involved in symbiotic interaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030448">
        <property type="term" value="P:hyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2430">
        <property type="entry name" value="Agglutinin-like protein, N-terminal domain, N2 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008966">
        <property type="entry name" value="Adhesion_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024672">
        <property type="entry name" value="Agglutinin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043063">
        <property type="entry name" value="Agglutinin-like_N_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033504">
        <property type="entry name" value="ALS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011252">
        <property type="entry name" value="Fibrogen-bd_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793:SF2">
        <property type="entry name" value="AGGLUTININ-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793">
        <property type="entry name" value="ALPHA-AGGLUTININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11766">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01056">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49401">
        <property type="entry name" value="Bacterial adhesins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442423" description="Agglutinin-like protein N-terminal domain-containing protein" evidence="13">
        <location>
            <begin position="18"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Agglutinin-like protein N-terminal" evidence="14">
        <location>
            <begin position="53"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW32687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="437" mass="48508" checksum="A365A13DE05D4A3F" modified="2011-11-16" version="1" fragment="single">MFSKILAFIFLATYVATTQVTGVFTSLDKVEFKPYSNKDPARPYQVHWEATMSWEIQGSKIQPLDTFTLDLPCVFKFITDEPYIALTVGSTEYAHCYFQAGEVFLTFSQLNCVALSSVKDSTDAHGTVRFPFTFNAGGSSLDVDLQCSKKFTSGSNIISFYEGDKEFRYSVNFVTQLLTHTDDITFHARSIPTLNRLQTYVVAGKCTKGYTSGTLGFEMLDSSSKIDCNSYHASMSSQFNSWMMAGTAENMEMTVSCTDSVLIVEYQNIPVGYRPYLDANLAVQEGAATPMIYHNSYTCVGSRYETDNDKDHEWSKYSNSNADSEGMEVVFATSTWTGQVTQITTLPFSTGEHYTKSIIVQVPIPTVTTTTIYTGVSTYWSTITATPGETATIIEHSPTDTTTTITQCWNKDYTTSTIILDTISAIHTLEVSIPCDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALT7</accession>
    <name>G3ALT7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Stationary phase protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60054</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374211.1">
        <property type="nucleotide sequence ID" value="XM_007374149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALT7"/>
    <dbReference type="GeneID" id="18873200"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60054"/>
    <dbReference type="eggNOG" id="ENOG502S2SB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_741848_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALT7"/>
    <dbReference type="OMA" id="CYIDFPI"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038816">
        <property type="entry name" value="Stationary_phase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42342">
        <property type="entry name" value="STATIONARY PHASE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42342:SF1">
        <property type="entry name" value="STATIONARY PHASE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="418" mass="48446" checksum="F8B2E37D948A40E7" modified="2011-11-16" version="1">MSMQRLTRNISKRIQRVLEEIAERLAHPHQLQPVPIPVRVPVPVRGRPRGPHGIPHRSNFACPGLQIRRFFCSFTGGNPNWTYHQMNSNFPGRGKTWFGKYNIFQRFYSFAGKRAVGGSRILQSLDHSFMFHNFSQAYQSNFRLKLYQQNVRLTYRTIFNRLREKYQIYGGLTQANVFAGTNHTFNYKQKFNPTIRLNLSLTPQHHKITLQLARSDSSATTQEKLECNQLNVGCYIDFPINFNLNIPQETILTEETFEEMMFNIKMFEQKLKDLKKDLTNLFELGELPIKYISDKNVLRIYFPNCDREKLENLCKEKNVTGGIIFEEDCENYPEPAMMMAPSSSGSVGESDILSCYYGTNESESSCESQSDLFSTSEEFHPLSEDDIVRPEFAMGTMPINMDPVISDCDEYYWISSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALV1</accession>
    <name>G3ALV1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_149801</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374225.1">
        <property type="nucleotide sequence ID" value="XM_007374163.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALV1"/>
    <dbReference type="GeneID" id="18870842"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_149801"/>
    <dbReference type="eggNOG" id="ENOG502T69B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_990444_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALV1"/>
    <dbReference type="OMA" id="ITTGMIW"/>
    <dbReference type="OrthoDB" id="4019667at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="317" mass="36761" checksum="D50329B6D6BD4230" modified="2011-11-16" version="1">MIRGKSISLKVISDKLIQLVPELLLLQVFYSIFKFYVQYFTYLYIKLNNFLVTITNRFINKSISIKSNLIPSLTSIYKREGTLSDVWFACNLITIVCGSIWFYLQWQNNYKRFYAYDITTWTALIGYFTTSISQLNSIFKKSQPKMAWSKPSNSVVAFLKSETTFMLIITELILESTTNSYKIAFLVFHSVLMLLGLIECSHNKAPVSFIAPLKGLLLFSFCMVEILVMGVFWLETLKHGFDKALLFTVCYSLRWEDSRTNQIAKNIKSYIRQLRQNKLNESQPLQAPVPIEKSAIQEFKRLTSFGFDTFSIISDIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALY0</accession>
    <name>G3ALY0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Early meiotic induction protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_60668</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374848.1">
        <property type="nucleotide sequence ID" value="XM_007374786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALY0"/>
    <dbReference type="STRING" id="619300.G3ALY0"/>
    <dbReference type="GeneID" id="18873516"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60668"/>
    <dbReference type="eggNOG" id="ENOG502S4MN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114639_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALY0"/>
    <dbReference type="OMA" id="QREKFWF"/>
    <dbReference type="OrthoDB" id="2017405at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021475">
        <property type="entry name" value="Pants/Emi1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28052">
        <property type="entry name" value="UPF0545 PROTEIN C22ORF39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28052:SF1">
        <property type="entry name" value="UPF0545 PROTEIN C22ORF39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11326">
        <property type="entry name" value="PANTS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="17639" checksum="43A3DC8542937B0B" modified="2011-11-16" version="1">MSDNNDKELEEVWKLFQDSPEEIQQRRKTLTKAIHDDKTLDYPSTVKVTTALDEVLECFALGGQLRHYYRYGTYDVCERQREKFWFAVKHGGFTDNNKPDDQLTEKELNTRLKIQEFFRKRVLEDKAAGSSEDIWDQRKELLSNPFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALY5</accession>
    <name>G3ALY5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcription factor IIIA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_135830</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374853.1">
        <property type="nucleotide sequence ID" value="XM_007374791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALY5"/>
    <dbReference type="FunCoup" id="G3ALY5">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALY5"/>
    <dbReference type="GeneID" id="18869925"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135830"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044102_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALY5"/>
    <dbReference type="OMA" id="SFYKHPQ"/>
    <dbReference type="OrthoDB" id="4748970at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001010">
        <property type="term" value="F:RNA polymerase II sequence-specific DNA-binding transcription factor recruiting activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001002">
        <property type="term" value="F:RNA polymerase III type 1 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042791">
        <property type="term" value="P:5S class rRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000125">
        <property type="entry name" value="Putative zinc finger protein 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002391">
        <property type="entry name" value="Transcription factor IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="24"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="54"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="110"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="139"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="196"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="345"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="388"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW33338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="433" mass="49543" checksum="E4CF617B2CF3E5CE" modified="2011-11-16" version="1">MSELDTVSLSSRTSSSGGSRPKKFLCTFENCDKAYSKPSLLEQHMRSHTNERPFKCSHEDCDKSFLRKSHLDAHMVSHATDKPFHCSVCGKGVNSQQHLRRHEITHSKSFTCTYEGCSEAFYKHQSLRHHILSVHEKTLTCTHCNKSFQRPYKLAQHNLKHHGDSPAYQCDHAGCFSNFKTWSALQFHVKQEHPKLKCSICGKGCVGRKGLRSHMLSHDEQKMLKLWQCTYCDIGKFPRKAELVNHYNDFHDGNIPENLLKRSELMRLEQLLNSSDPLSANSKDLKNYKIVSSDEDSDEDNDVAVDARSDTTMSQRSMNSLSKDLSKGRSSIIGLISNNFVSKKIKCPKGNCGRMFSREYDLDRHLKWHEENLSKIEAFLDNLNNETADLDEQPPLKKTKPNQGINLIDDHSDDDLDTLSHQELQLVAEFTED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM37</accession>
    <name>G3AM37_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_65786</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374257.1">
        <property type="nucleotide sequence ID" value="XM_007374195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM37"/>
    <dbReference type="STRING" id="619300.G3AM37"/>
    <dbReference type="GeneID" id="18875062"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65786"/>
    <dbReference type="eggNOG" id="ENOG502QQ5H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_291507_0_0_1"/>
    <dbReference type="InParanoid" id="G3AM37"/>
    <dbReference type="OMA" id="LANNMFD"/>
    <dbReference type="OrthoDB" id="7451790at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF22">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN COMPLEX ACID LABILE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="71"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="980" mass="109802" checksum="36AC9A3EDD999302" modified="2011-11-16" version="1">MSSLTTSIDNLMTEASDVFSKNSPAKILIDAANSEQRLQSIPDKHNESNEQESNVAHPDNSIDKSNPVSVETDDGGYDNEDEQENRSDTDSHFHRTVLQSHVIINRPQSNHSLLAEFAVGSSAATNENVDPESLFGFPDSAIFRKNQTSGNAFKVQDLNGEPIELETATGSEVKVEGLDSALANMSVSSIIKDDFNSPVKEVLDEVAVTETADQSVITSSTVEPPLESSTPKQNPVVEALRVEPKMKEFKLISPSKNFIENTAIVANSKLDEYSWISNDYNNLFGNKSFTREINLSKLLRFEEKLPNLLDENDIYISTRILNPGKILSGFFKDGNKRNVYIDLILNNNGFTIFSQILKYFSEHEKEANSKIIDILVRISATTVEQLRYFVEKFSESKVYQKVKLLGLFSGLHVYSDNNFPIEVSDEKLKLPAKLEKLCLSAGLNVTDFVQLPYSIKHIGLYNVPSLNVSKFNNKRLDCVHISSGDPIEFIGRQDNFPKSFLDLKDLKLDSSIVVNPQFDKFSHLVNLSIRNLDFATTRQLTFPSCLKKLEFIDCCISSHISSFPDSLKTLIVKGGKWNKKRNCNLTRVQKLTVVKADVKDLHEKLVSARSLLYLEIVDVEVDKLELPSGLKNLKLLSVNLSLFDVRNFPNGLETVVLDDNKISWTDHLVKLKQVKSLKITNNGIYGRVSLSECSKLEDVAVYDNDGLLAIKLPNSVKSLNVANTKVHEICGDNLTKLNVNGCTKIDWESFNIPASVTHLTAQGCGLTRFEYGIRNDGLKVLNIANNNIEQLDLHKFNSIQDLDLSSNQLKRLVKLPLSLKSINAGNNRISEVDVAMLETLEWLELSNNHITSLTRVHVPICLRTLLLNNNQFGRFAQHEFDIPSKLCHLELNGCNLSEFVQVLPSSLLSFCASNNKLNRLRFRFVFASVSNLKYVDLHSNIFDSFDFSLFKGIEIAELNLSDNLFAEAPNIPDNILTALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMB9</accession>
    <name>G3AMB9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_136811</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374932.1">
        <property type="nucleotide sequence ID" value="XM_007374870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMB9"/>
    <dbReference type="FunCoup" id="G3AMB9">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMB9"/>
    <dbReference type="GeneID" id="18870001"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136811"/>
    <dbReference type="eggNOG" id="ENOG502QQ2N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007860_1_0_1"/>
    <dbReference type="InParanoid" id="G3AMB9"/>
    <dbReference type="OMA" id="DPCIFIV"/>
    <dbReference type="OrthoDB" id="2427554at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016287">
        <property type="term" value="F:glycerone-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090207">
        <property type="term" value="P:regulation of triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052744">
        <property type="entry name" value="GPAT/DAPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31605">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31605:SF2">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="470"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="505"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="2">
        <location>
            <begin position="51"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="684" mass="78265" checksum="8573F9320D5AB65B" modified="2011-11-16" version="1">MTSIIESINESFRYLIYDLVLQFFNLLVHTFFRDVKTRGTFHIPPHGPVIFVIAPHHNQYIDGLLVMSQVKAHSGRHIAFLIAGKSYRMNIVGHGARLVSAIPVERAQDLLKKGTGKIYLDTSDKTGHSLIGEDTKFSSECEPRGLIGLYNSLGNCQIDKVIDDTHLVLKDPFFSSRNEIQTKIDIALKQGTPYKIAPHIDNHHVFNHVFHHLAQGKVLGIFPEGGSHDRPDLLPLKPGVAIMALGAVSEQIKSNKQVTPINVIPVGLNYFHAHRFRSRVVIEFGRPIVVDEKMGHEYDTDRFQAVDKLLETITAKLKEVTTTSEDYDTLMVLQAIRRLHTSSNREYIPLPMVVEMNRRLSRSYEKYKDEPDVIELRDSVTDYNNLLMRYGLHDHHIETLHQSNFFRTFIIFLRRFSTFCIYMGLSLPGMVLFSPVFITAIRISREKQKQALAASSVKIRARDVISTWKCLVAMVLAPALYIFYSIIGTVYIVRNNILPHISVGWIFFSCYCLALMTTYASLRVGEIGVDYYKSLRPLLVSMISRKKDRIQIAELKLQRERLAIKVNDFCMKYNPSIFADYNRLYGPDGFEDHYQYQSDSEIEEKRKLIRRNSSISLDFDNMGSIPIFSSEDLERIYDDDKKNYSTESSMDEADTVSDDLNMATGIANGDSNVRLRKTLFHEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMY8</accession>
    <name>G3AMY8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cytochrome P450 52A3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_153278</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007375616.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="spaa:SPAPADRAFT_153278"/>
    <dbReference type="eggNOG" id="KOG0157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_27_0_1"/>
    <dbReference type="InParanoid" id="G3AMY8"/>
    <dbReference type="OMA" id="PPAFSYI"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="CYP52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR002974">
        <property type="entry name" value="Cyt_P450_E_CYP52_ascomycetes"/>
    </dbReference>
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        <property type="entry name" value="Cyt_P450_E_CYP52_fungi"/>
    </dbReference>
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        <property type="entry name" value="Cyt_P450_E_grp-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287:SF1">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EP450II"/>
    </dbReference>
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        <property type="entry name" value="EP450IICYP52"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602402-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="515" mass="59132" checksum="F8BF6AE47D0D1FA8" modified="2011-11-16" version="1">MQISTACVLNSTVKFFTSWYGIIISSILVYALAIHIKRERFRRKHGTQEPPRFREGGFLGLFLAYTLISFKQSGELLDFAVSILDRYNGTLSAKLFGLRVIATREPENIKAMLATQFNEFGLGRHSHLATLLGDGIFTLDGDGWKHSRQLLRPQFAREQVAHVQKLEPHVQTLAKHIRKYKGDKFDIQELFFRLTLDTATEFLLGESVHSLHDDSIGLPARKEVLEFARAFDLSQKYLGSRVYSQAAYFLVDGFEFRRANRTVHNWIQTFVTKALTMTPDVLEEKSKDGYTFLYELVKYTKDKRVLQDQLLNILLAGRDTTAGTLSFCLFELARNPDVWEKLKAEIYQTFGKDDTSKITFESLKQCEYLKWVINETLRLYPSVAVNFRVPNKDTTLPVGGGPDGTAPVFVAKGTTVAYSYYVTHRMEKYFGKDPTRFIPERWEHLDQLGWAYLPFNGGPRICLGQQFALTETGYVLTRLIQMFPNLETRDHRHYPPLKNCQLTARHQDGVFISLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN07</accession>
    <name>G3AN07_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein mL43</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_61493</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL43 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007375635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN07"/>
    <dbReference type="FunCoup" id="G3AN07">
        <property type="interactions" value="382"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN07"/>
    <dbReference type="GeneID" id="18873918"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61493"/>
    <dbReference type="eggNOG" id="KOG3445">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117700_1_1_1"/>
    <dbReference type="InParanoid" id="G3AN07"/>
    <dbReference type="OMA" id="ISKWIDL"/>
    <dbReference type="OrthoDB" id="88at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_mL43"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_mL43/mS25/NADH_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
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        <property type="entry name" value="39S RIBOSOMAL PROTEIN L43"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein/NADH dehydrogenase" evidence="7">
        <location>
            <begin position="32"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="140" mass="15811" checksum="FD12D61EAE865CCD" modified="2011-11-16" version="1">MPIKAIPKISLARNGVGAFVAPCHRITVQYCNWGGSSTGLRQVLSSGQLDHIARQKKYTFFEVVKKSGHPSLTFHYNNDTTQVVDIANLDSEKIVEKLEEYSQRSGNKLIKFNHKVMSNNDSVRGIWSPLHMPKGHRHKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN14</accession>
    <name>G3AN14_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ketopantoate reductase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_67043</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375704.1">
        <property type="nucleotide sequence ID" value="XM_007375642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN14"/>
    <dbReference type="GeneID" id="18875134"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67043"/>
    <dbReference type="eggNOG" id="ENOG502QT3Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_316416_0_0_1"/>
    <dbReference type="InParanoid" id="G3AN14"/>
    <dbReference type="OMA" id="IKKMDCK"/>
    <dbReference type="OrthoDB" id="5302359at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051402">
        <property type="entry name" value="KPR-Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708">
        <property type="entry name" value="PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708:SF25">
        <property type="entry name" value="PROTEIN PAM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="3">
        <location>
            <begin position="251"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="490"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="544"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="595"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="412"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="492"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="600"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="626"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="714"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="787"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="835"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="852"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="882"/>
            <end position="892"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="892" mass="100527" checksum="EEF4061A984D1280" modified="2011-11-16" version="1">MISEKRLSQVPVANCSQSFPTVPNCSYRELLTRNLLQSAQDLCECRRMPHPTPPRTKLLTNVLVGSNPNIAFYAWQFHQTNAVEITIVNNELNPTAPVSWKSSVFGNSQYTPHRIYNSLTQVPDNKYDIFLLSCSSLQDFQNVSNEIVPFIDRDSIIVIESTGYINLEPFVSTKAQVLSIMNESDVKRVSKNVYSHEVRNKDTRIYFGSALGSKSISSNMNYHRIYKLLQTIQEDSNNHISLLKSLTPTEFMTYQWKLALPRIIFSPLMVIFETEFPQDLQTQILSKPLITGLINELFKLIKKMNCKLVKGFENELNLLKGLSESYPKKSSDKFVNSPSLVYNYYNHYDLELDLLLLQPILLSDDHKLKSPYLENLYSIMCQYHKINSNDSIFFVRKDSAKFDQYLSLDEDLKQKTYQLSQLEGSIKELERNKINLDNDIIKQEHSLKTLKQQTIENESKLSTVQYEFDKKLQILQIDYDKKLKQLNEKYQKAQSSTSSDVSIPLQQPTPGTDDHSTNRDSVVTNNLTDLADIVQYGMALNGEHQPSEVPQTIPGDAVAAPRNVSPDRYVDAPSVSLPPHVVQKELELQRREQALAMREQQQQQQQQPHYPQQPYYDQQYYAPGPTHGPGPMPGSVPPAPHQQRSYYQQPPHGLPPNGMSVTSLPLKQGYPPNKQSYPMAPGAGSMNGYHHPPPPQPRRVSSSTNTAYYDHPQQYPPYQQPPPPQPQQQPAPPSGYDNGAPIDPYLEHRFKQNPKKQNRRSNMGLNMEGIDMGGRGGMPGAAPTARKSFSSQQLNSPTSMTKRSTTTMNLSTTHPNGSYSQSSSDGNNPHHHHGSQNYSQSSSSGTSNGSNDNIPTSPSINDLNAKPLGGISQSNMPGGDKLKKKKKGIFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN22</accession>
    <name>G3AN22_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Glutamate decarboxylase</fullName>
            <ecNumber evidence="3 9">4.1.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_61506</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 9">
            <text>L-glutamate + H(+) = 4-aminobutanoate + CO2</text>
            <dbReference type="Rhea" id="RHEA:17785"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="4.1.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.15" evidence="3 9"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375713.1">
        <property type="nucleotide sequence ID" value="XM_007375651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN22"/>
    <dbReference type="FunCoup" id="G3AN22">
        <property type="interactions" value="328"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN22"/>
    <dbReference type="GeneID" id="18873923"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61506"/>
    <dbReference type="eggNOG" id="KOG1383">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019582_2_1_1"/>
    <dbReference type="InParanoid" id="G3AN22"/>
    <dbReference type="OMA" id="FIVPFTH"/>
    <dbReference type="OrthoDB" id="5152799at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004351">
        <property type="term" value="F:glutamate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010107">
        <property type="entry name" value="Glutamate_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01788">
        <property type="entry name" value="Glu-decarb-GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321:SF3">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="348" mass="39298" checksum="AA795AD92E0973F1" modified="2011-11-16" version="1" fragment="single">MLAGLALKKRWQEKRKAEGKSIAEPNIIMATCAQVALEKFARYFDVENRLIPVTAESGHVIDLSKIKENIDENTIGIFVIMGSTFTGAFEPVEAISNLLDEVEKETGLDIRIHVDGASGAFVAPFVFSHLKWDFSIPRVDSINTSGHKFGLTSCGLGWVIWKDANLLPQSLKFNLDYLGGVEETFGLNFSRPGFPVITQYYNFLTLGREGYRKVFDGCLSNARLLSNFLEGSKYFDVVSVIHHTLTAEEQKKTYTKHDGEDFQVNEQYKPGLPVVAFTFSQEVQEKYPELPQSIFSSLLRRRGYIVPNYHLPPDENDKEILRVVIRTSMSLGLLEKLLQDCVETMELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN60</accession>
    <name>G3AN60_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61006</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375179.1">
        <property type="nucleotide sequence ID" value="XM_007375117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN60"/>
    <dbReference type="STRING" id="619300.G3AN60"/>
    <dbReference type="GeneID" id="18873677"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61006"/>
    <dbReference type="eggNOG" id="ENOG502S01E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087023_0_0_1"/>
    <dbReference type="InParanoid" id="G3AN60"/>
    <dbReference type="OMA" id="WDSMDYR"/>
    <dbReference type="OrthoDB" id="4010386at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012470">
        <property type="entry name" value="Pup1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07954">
        <property type="entry name" value="DUF1689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="29224" checksum="FF9A8262A0E0EE52" modified="2011-11-16" version="1">MSKETPSMSTSRDNPLFDKLNPQQIQASIKFYEADHELTHEERLKITQDLQYMVGKSNIVTLGATIAGSLSPTVYYRYIKKQVPAGARFIVYPGLSFFLGLGNLFLVNNMYGRYQFNQKQNEVHDPKLQRVWDSMDYRTLAIFYIYYLRSSADPNAIMKDPRSITREDIYGVKVDPNMAEKVNQPKDTYSEYATRWNEIRQEEVKHEQELPEIKKYDNDDIFDFDKPRTETSDQEDHQSAWDKIRSSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANK4</accession>
    <name>G3ANK4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61597</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007375747.1"/>
    </dbReference>
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        <property type="interactions" value="199"/>
    </dbReference>
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    <dbReference type="eggNOG" id="ENOG502S0M8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126647_1_0_1"/>
    <dbReference type="InParanoid" id="G3ANK4"/>
    <dbReference type="OMA" id="WYRVVAF"/>
    <dbReference type="OrthoDB" id="1078367at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
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        <property type="entry name" value="Primosome_PriB/ssb"/>
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        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
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    </dbReference>
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        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="135" mass="15622" checksum="C141203038D8F905" modified="2011-11-16" version="1">MLRSFTRSLSSTAPRFNFARASFVGTVGHSEIKTSEKGVKYAKYSLAVHRYPKDREPETEWFNITAFDRVDSLEKVLRPGNLMHVEAHIQTTKLIDEDNKASFHTNYVQRSYDVLRFGRRPEQAEDAESEEQGHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANP3</accession>
    <name>G3ANP3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Matrin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_61091</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007375192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANP3"/>
    <dbReference type="FunCoup" id="G3ANP3">
        <property type="interactions" value="1254"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANP3"/>
    <dbReference type="GeneID" id="18873706"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61091"/>
    <dbReference type="eggNOG" id="KOG2636">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027160_1_1_1"/>
    <dbReference type="InParanoid" id="G3ANP3"/>
    <dbReference type="OMA" id="IPHWLYK"/>
    <dbReference type="OrthoDB" id="2160351at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
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        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
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        <property type="entry name" value="PRP9_N"/>
    </dbReference>
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        <property type="entry name" value="RNA_Proc_DNA_Dmg_Regulator"/>
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        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786">
        <property type="entry name" value="SPLICING FACTOR SF3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SF3a60_Prp9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="7">
        <location>
            <begin position="423"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="490" mass="56038" checksum="103495DC378C4A4F" modified="2011-11-16" version="1" fragment="single">MSGFIESQRAALEELDVIEEAISSRLGRDPHLLPPSLRPKKDIISKRKPKKTSTLRLSLLQQSELRFFIEKYHRTHSSLEFCLANEKLVLQKQLANVTEGPKQFAKFDQMISEISNKESDHPSGAIAEDPRNLYSSFSSITRDKESHSQDLKIKVEKNTGKQRELTKRKFILSSVASHLQDSIENEGYLDLSGVHIQYNSQLASTPISYVQYLYTFQNLEAPAKGTPYVKYIRDLANLLAESFKNLHPLTDLDQLENAWKQEFQETNSTSEGQENEKGEVYCKACDKLFSKMSVYKGHLDGKKHKKNAAKSSGDSASVDSFDSLTWYEFAIGKLVSVLQTDIEAARAHAERIANSSERELLMEQQNLEEIESEFTDIGDYSDFGDNSDEEYEDNETFKNLPLGPDGAPIPFWLYKLQGLNKKYVCEICGNITYKGRQIFLKHFSGVKHQYGLKCLGIEEGYISFFKNITNIEEARILWTKFKREKREKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANS8</accession>
    <name>G3ANS8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_61116</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375289.1">
        <property type="nucleotide sequence ID" value="XM_007375227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANS8"/>
    <dbReference type="FunCoup" id="G3ANS8">
        <property type="interactions" value="576"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANS8"/>
    <dbReference type="GeneID" id="18873721"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61116"/>
    <dbReference type="eggNOG" id="KOG0684">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_15_0_1"/>
    <dbReference type="InParanoid" id="G3ANS8"/>
    <dbReference type="OMA" id="FPNPLEW"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008398">
        <property type="term" value="F:sterol 14-demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11042">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF24">
        <property type="entry name" value="LANOSTEROL 14-ALPHA DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="385" mass="44077" checksum="A789E14EE1C26A78" modified="2011-11-16" version="1" fragment="single">MEQKKFAKFALTTDSFKRYVPQIREEILKYFTSDFHMKESDSGVANVMKTQPEVTIFTASRSLMGEAMRNRFDKSFAKLYSDLDKGFTPINYVFPHLPLPTYWRRDESQRKISGTYMKEIKHRREVGDIDPSRDLIDSLLVHSTYKDGVKMTDQEIANLLIGILMGGQHTSAATSAWFLLHLGAKPELQEELYQEISAILKEKGGDLNDLTYEDLQNMPLVNNTIKETLRMHHPLHSIFRKVMKPLLVPNTNYVVPKGHYVLVSPGYAQTNDRYFHDAADFNPKRWDEKTNLENLKAISDASGEVDYGFGKVSKGVSSPFLPFGGGRHRCIGEQFAFVQLGTILTTYIYNITWTCDKVPNQDYTSMVVLPEAPAVIHWKKRETCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP09</accession>
    <name>G3AP09_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_66706</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375316.1">
        <property type="nucleotide sequence ID" value="XM_007375254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP09"/>
    <dbReference type="GeneID" id="18875110"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66706"/>
    <dbReference type="HOGENOM" id="CLU_729914_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP09"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="379" mass="41515" checksum="5F1EEEC9D03B044D" modified="2011-11-16" version="1">MVQNEVLENYGFDDHSDTTITSTITLTVTNYSSSTINCATTEERLDQIVFAEEDYMAFEASEITETTTTTVTLINSITTTATTCTKSLAEETQSNTFNHDQPFNIIGSYNDTEFLFTINGTSITLSCEPGEFSLEEGYLKVANEAVQISHDGKLVIGGEGSLGWSVVDEKLMLTTTTTKCNTTISQAVEFQVCREGNMYWVGIGSDPDCESIEAVLSQSAHQQQDETSCNNQDAGEVLKKILFQGILEDDYVTLDDTPTETPTLDFYSTTTVTKSATTTIITACSGKEEHAASPELLAADSVSLEPNLYTSVTITLTIFSTVPDVMSTPTPKPVTPTNSSKEYDPIAYLLENRSVSIKRINKTTILFYLIVHALVQMFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3APB3</accession>
    <name>G3APB3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GSKIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_50684</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375366.1">
        <property type="nucleotide sequence ID" value="XM_007375304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APB3"/>
    <dbReference type="STRING" id="619300.G3APB3"/>
    <dbReference type="GeneID" id="18871474"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50684"/>
    <dbReference type="eggNOG" id="ENOG502T9YZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155352_0_0_1"/>
    <dbReference type="InParanoid" id="G3APB3"/>
    <dbReference type="OMA" id="NIESHIH"/>
    <dbReference type="OrthoDB" id="5804279at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2280.10">
        <property type="entry name" value="Hypothetical protein (hspc210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007967">
        <property type="entry name" value="GSKIP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023231">
        <property type="entry name" value="GSKIP_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05303">
        <property type="entry name" value="GSKIP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103107">
        <property type="entry name" value="Hypothetical protein c14orf129, hspc210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GSKIP" evidence="1">
        <location>
            <begin position="77"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="115" mass="13112" checksum="2C818AD181E8D403" modified="2011-11-16" version="1">MDIDTQVYELTTIHKEYESFFPQCQLKLGNVSVAARSYSDNGELLPASKDNYIYIQTQEGIDLKVSVELRGWYQLGAPKKSYETFEGFMQAVSPSFQVKFGDELSNKLLQVLQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3APE8</accession>
    <name>G3APE8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-N-acetylhexosaminidase</fullName>
            <ecNumber evidence="3">3.2.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_61209</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.</text>
            <dbReference type="EC" id="3.2.1.52"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 20 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.52" evidence="3"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375401.1">
        <property type="nucleotide sequence ID" value="XM_007375339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APE8"/>
    <dbReference type="STRING" id="619300.G3APE8"/>
    <dbReference type="GeneID" id="18873778"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61209"/>
    <dbReference type="eggNOG" id="KOG2499">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007082_0_0_1"/>
    <dbReference type="InParanoid" id="G3APE8"/>
    <dbReference type="OMA" id="IREIVHY"/>
    <dbReference type="OrthoDB" id="428480at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016231">
        <property type="term" value="F:beta-N-acetylglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030203">
        <property type="term" value="P:glycosaminoglycan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000063">
        <property type="entry name" value="Beta-hexosaminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025705">
        <property type="entry name" value="Beta_hexosaminidase_sua/sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015883">
        <property type="entry name" value="Glyco_hydro_20_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600">
        <property type="entry name" value="BETA-HEXOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600:SF26">
        <property type="entry name" value="BETA-N-ACETYLHEXOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00728">
        <property type="entry name" value="Glyco_hydro_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00738">
        <property type="entry name" value="GLHYDRLASE20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycoside hydrolase family 20 catalytic" evidence="9">
        <location>
            <begin position="10"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR625705-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="362" mass="41480" checksum="A7EDD89A2B1A8D2B" modified="2011-11-16" version="1">MINDAYSPREIYTKQDILYVINYAYERGVRIVPEVDMPGHARAGWRQVNPDIVTCGDSWWSNDVWANHTAVEPPPGQLNILMPETYEVITDVYEELSSIFPDNFFHVGADELQEPCYYMSPLIMDNWFEKNRTMNDLTQYYVDKALPIFFNVSADRKLIMWEDIITTPEGAHTLPTENIILQSWNNDLVNIKNLTSQGYDVIVSSASHFYLDCGYGGWVTNDPRYVDIPDNDDFNNGQGGSWCAPYKTWQRIYDYDFTANLTAEEAQHVVGAEVALWSEQVDSTVLISKIWPRTAALAESTWSGNRDDEGHLRTNLLTSRILNFREYLVALGHAASPLVPKYCLQNPHACDLYQNQTALDQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3APS0</accession>
    <name>G3APS0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tryptophan--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="3">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Tryptophanyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_61321</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tRNA(Trp) + L-tryptophan + ATP = L-tryptophyl-tRNA(Trp) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9671"/>
            <dbReference type="Rhea" id="RHEA-COMP:9705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78535"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="3"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375517.1">
        <property type="nucleotide sequence ID" value="XM_007375455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APS0"/>
    <dbReference type="FunCoup" id="G3APS0">
        <property type="interactions" value="425"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APS0"/>
    <dbReference type="GeneID" id="18873841"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61321"/>
    <dbReference type="eggNOG" id="KOG2713">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029244_1_3_1"/>
    <dbReference type="InParanoid" id="G3APS0"/>
    <dbReference type="OMA" id="GWGQFKP"/>
    <dbReference type="OrthoDB" id="15808at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070183">
        <property type="term" value="P:mitochondrial tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000082">
        <property type="entry name" value="MSW1p Mitochondrial tryptophanyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000002">
        <property type="entry name" value="Tryptophan--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00140_B">
        <property type="entry name" value="Trp_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024109">
        <property type="entry name" value="Trp-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050203">
        <property type="entry name" value="Trp-tRNA_synthetase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43766">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43766:SF1">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00069760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW32241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="370" mass="41497" checksum="C861E36EDAAD591B" modified="2011-11-16" version="1">MTVGKTIASKAARTVQEISKNQHLPNNSRVFSLIQPTGKFHIGNYLGAIQSWKTLSETESPGTQYIFGIADLHAITTGVKPAGELRSNRHEAMAGILSCGLNPKKCILFHQSSVPEHAELHWYLTCLTSMGALNRMTQWKSKARQLGDDESDQLKFLEHTKAGLLCYPILQAADILIYRSTHVPVGDDQSQHLEMCRSVAINFNNSYKTNFFPIPQTLLTPSKKILSLRNPLKKMSKSDPDQSSCIYITDAPEVIAKKIRKATTDSVQGPVYFDPEARPGVSNLINILSGVTSQSIEQVVEDLAYVKDHKQFKDHVTDVLVTEFKQQRELFEELMKDGVYLEKVCHEGRDKAREIATKNIEEVRKLIGMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3APT7</accession>
    <name>G3APT7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Trimethyllysine dioxygenase</fullName>
            <ecNumber evidence="5">1.14.11.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Epsilon-trimethyllysine 2-oxoglutarate dioxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">TML hydroxylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">TML-alpha-ketoglutarate dioxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_138818</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Converts trimethyllysine (TML) into hydroxytrimethyllysine (HTML).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(6),N(6),N(6)-trimethyl-L-lysine + 2-oxoglutarate + O2 = (3S)-3-hydroxy-N(6),N(6),N(6)-trimethyl-L-lysine + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:14181"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:58100"/>
            <dbReference type="ChEBI" id="CHEBI:141499"/>
            <dbReference type="EC" id="1.14.11.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amine and polyamine biosynthesis; carnitine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the gamma-BBH/TMLD family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.8" evidence="5"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375535.1">
        <property type="nucleotide sequence ID" value="XM_007375473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APT7"/>
    <dbReference type="STRING" id="619300.G3APT7"/>
    <dbReference type="GeneID" id="18870153"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138818"/>
    <dbReference type="eggNOG" id="KOG3889">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021859_2_2_1"/>
    <dbReference type="InParanoid" id="G3APT7"/>
    <dbReference type="OMA" id="EKVCIQP"/>
    <dbReference type="OrthoDB" id="408743at2759"/>
    <dbReference type="UniPathway" id="UPA00118"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050353">
        <property type="term" value="F:trimethyllysine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045329">
        <property type="term" value="P:carnitine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00250">
        <property type="entry name" value="CAS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2020.30:FF:000002">
        <property type="entry name" value="Putative gamma-butyrobetaine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000001">
        <property type="entry name" value="Trimethyllysine dioxygenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2020.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050411">
        <property type="entry name" value="AlphaKG_dependent_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010376">
        <property type="entry name" value="GBBH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038492">
        <property type="entry name" value="GBBH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012776">
        <property type="entry name" value="Trimethyllysine_dOase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02410">
        <property type="entry name" value="carnitine_TMLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696">
        <property type="entry name" value="GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696:SF51">
        <property type="entry name" value="TRIMETHYLLYSINE DIOXYGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06155">
        <property type="entry name" value="GBBH-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0124">Carnitine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma-butyrobetaine hydroxylase-like N-terminal" evidence="18">
        <location>
            <begin position="9"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="TauD/TfdA-like" evidence="17">
        <location>
            <begin position="143"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00071191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF06155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW32259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="398" mass="46082" checksum="FD8B0A0200629BFB" modified="2011-11-16" version="1">MNFTVTATNQGNDQLELTWSDGKTSKYHYIWLRDNCHCSTCYFPTTKQKLASAATIDDHIAPKNVTVGNSDIDIVWNQEDHLSTYDFHWLRVHSYNPRLIPLSKKLKGETELLSRQLWQVADIENDLPQVEYSNVMNSSEETDNEEAIKDWSLKLWKYGFCLINNVPVTPEDTEKLCEQLNYIRPTHYGGFWDFTSDLAKADTAYTNFDISSHTDGTYWSNSPGLQLFHLLFHDGTGGTTSLVDAFKCSQILKEEYPDSYELLCRIPVPAHSAGEEKVCIQPDVPQPVFKLDSQGNLVQVRWNQSDRSTMDNWDNPDDVPRFYKAIKDWVRIISDPKNEFWYQMKPGQCLIFDNWRVFHSRSEFTGKRRLCGAYLERDDFVSRLKLLVLGREAVLDSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3APV9</accession>
    <name>G3APV9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_152987</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375555.1">
        <property type="nucleotide sequence ID" value="XM_007375493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APV9"/>
    <dbReference type="GeneID" id="18870937"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_152987"/>
    <dbReference type="eggNOG" id="ENOG502RQB0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075354_0_0_1"/>
    <dbReference type="InParanoid" id="G3APV9"/>
    <dbReference type="OMA" id="ARFKNCI"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011999992" evidence="1">
        <location>
            <begin position="16"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="253" mass="28964" checksum="C79FD9AF5309DB8C" modified="2011-11-16" version="1">MILLNFLIFVQFACSFTILPFKNEIKPNLKYLAYQSGTRYIETVDSLSQINPKRLFKYGVIKLGNNSAVFSEELAYSTMSANYIPVYEKHQDYSKLKHKKHKYYEVSYDDARAVIEISKWKPIGKCVINHSNETAVYSQGWQIDISSSLNFRIDFAALITTFRPNAKSELALTKSIGGGLSCDVEPGNSLQFALITESYKITGVKQREIKITMFGLKAEPWEEVPPFTRINRKNIQVACFTDNQLETCEEDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQJ1</accession>
    <name>G3AQJ1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62130</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376316.1">
        <property type="nucleotide sequence ID" value="XM_007376254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQJ1"/>
    <dbReference type="GeneID" id="18874194"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62130"/>
    <dbReference type="HOGENOM" id="CLU_2711801_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQJ1"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="73" mass="8310" checksum="EF0772A858640937" modified="2011-11-16" version="1" fragment="single">LVMNKSKITWTHQDLAQYLCLQLRIKHVCIICGDTPINMLIHSTYKNNWLSYFISDENETVTGGIGQGVSILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQK7</accession>
    <name>G3AQK7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-splicing factor CLF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_140880</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the crooked-neck family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376332.1">
        <property type="nucleotide sequence ID" value="XM_007376270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQK7"/>
    <dbReference type="FunCoup" id="G3AQK7">
        <property type="interactions" value="1096"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQK7"/>
    <dbReference type="GeneID" id="18870326"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140880"/>
    <dbReference type="eggNOG" id="KOG1915">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011554_1_0_1"/>
    <dbReference type="InParanoid" id="G3AQK7"/>
    <dbReference type="OMA" id="HIKVWIS"/>
    <dbReference type="OrthoDB" id="541719at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071008">
        <property type="term" value="C:U2-type post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000354">
        <property type="term" value="P:cis assembly of pre-catalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055433">
        <property type="entry name" value="HAT_Syf1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF3">
        <property type="entry name" value="CROOKED NECK-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23233">
        <property type="entry name" value="HAT_Syf1_CNRKL1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats" evidence="9">
        <location>
            <begin position="52"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="701" mass="83183" checksum="23823B3160CE2175" modified="2011-11-16" version="1">MDAAEAIPPNQITSKGILQEAYKQKESKFTRPQQTIQDLDELQSFQQAKRKEYEQQLNKNRLNYGEWLRYARWEVQHNHDFPRARSIMERALDVNNQHVPFWVSYIQLELSHKNINHARNLLDRAVKILPRVNKLWFLYVQTEESLKNYHVVRNVFERWLRWHPEPAAWDAYVSFESRYEEYDNVRNIFIRYIQEYPKGETWNKWVDFELENSVENVNSIRAVFESAVDTLLVRVDENDPYLPVIISRWADWEVSCDELERAREIFELITGDRLNLPASFRDSINELFTQFEKVHGTKDSIQSSTVLKRKERYESDIAQDPTDYDSWWKYITILQSNQRVATIQEAFKNALSNVPKDKYKSIKWRRYIMLWIRFALWEEIQNGDIEAARTIWDDCLKVIPHKLFTSGKVWKGLAEFELRNNSTEDNLAKARKVLGRAIGQTSANGPKNNILEYYINLETKLGEWDRVRLLFEKWLEIALTTSTSCLDILKQYVEFEQSLGEYQRCESILSLFLDLSNNKATAASFEPVDKFFHFCVEFYKDEMKYDEIRKMYRDLLEKSPSASNWISFALFESTIPSKEQLEEYLQSEADSFEVTLQDEQIENTRNLFEEAEKYFQDKQANEDRLVVIEAWKNYEEVHGDDESFDRVSKKIPTIVKKTRNVDGIEQEYLDYVFPEDKSDVSFSVNAFLANAKKWAAQSNDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR67</accession>
    <name>G3AR67_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane sensor/transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_61815</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetate uptake transporter (AceTr) (TC 2.A.96) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376020.1">
        <property type="nucleotide sequence ID" value="XM_007375958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR67"/>
    <dbReference type="STRING" id="619300.G3AR67"/>
    <dbReference type="GeneID" id="18874064"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61815"/>
    <dbReference type="eggNOG" id="ENOG502QUJS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051062_0_0_1"/>
    <dbReference type="InParanoid" id="G3AR67"/>
    <dbReference type="OMA" id="INTFAGW"/>
    <dbReference type="OrthoDB" id="3648309at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015123">
        <property type="term" value="F:acetate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051633">
        <property type="entry name" value="AceTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000791">
        <property type="entry name" value="Gpr1/Fun34/SatP-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038013">
        <property type="entry name" value="AceTr_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123:SF1">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01184">
        <property type="entry name" value="Gpr1_Fun34_YaaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="269" mass="28586" checksum="13899557AA9329AD" modified="2011-11-16" version="1">MASINSTVSNDSYSKPIPIHDEELKIHKTCSIAGPGNEFVIIGDNKYYRHELMQAFGGTFTSGLTPYPKRQIANAPALGLFATHMNIFVLGLIFAGATGGSIPNAAVGLFIFVGGLLQFLAGVWCMVGGNTLGATAFTSYGAFWLSFGAIFIPGFGIGAAYADDPEQLNQGIGLISVGWAIFTTMLLACLFKSTLSFFWTVLTLDLTIILLAAGFLSGSPKVMKAGGIMGVINAFAGWFETYAGIATKQNSYLVPKEIPLPDLSRKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR70</accession>
    <name>G3AR70_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_61818</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376023.1">
        <property type="nucleotide sequence ID" value="XM_007375961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR70"/>
    <dbReference type="GeneID" id="18874066"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61818"/>
    <dbReference type="HOGENOM" id="CLU_3088793_0_0_1"/>
    <dbReference type="InParanoid" id="G3AR70"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="52" mass="5999" checksum="2564B0BE40EC9E06" modified="2011-11-16" version="1">MRKSAIITVGEDQRLRQFLKARRYTYTGQISRKIPSARKKSLGDGIPAIREH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARC7</accession>
    <name>G3ARC7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_51716</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MUB1/samB family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376512.1">
        <property type="nucleotide sequence ID" value="XM_007376450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARC7"/>
    <dbReference type="FunCoup" id="G3ARC7">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="GeneID" id="18871585"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51716"/>
    <dbReference type="eggNOG" id="ENOG502QTM3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014851_1_0_1"/>
    <dbReference type="InParanoid" id="G3ARC7"/>
    <dbReference type="OMA" id="QDMQYWA"/>
    <dbReference type="OrthoDB" id="5594178at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990304">
        <property type="term" value="C:MUB1-RAD6-UBR2 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051664">
        <property type="entry name" value="MYND-type_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47442">
        <property type="entry name" value="MYND-TYPE ZINC FINGER PROTEIN MUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47442:SF1">
        <property type="entry name" value="MYND-TYPE ZINC FINGER PROTEIN MUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="9">
        <location>
            <begin position="697"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="149"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="459"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="502"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="742"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="475"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="510"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="742"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="755"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="779" mass="89371" checksum="3142FBD55FA10846" modified="2011-11-16" version="1">MRDSNYKAISSNKAAVTITTSLYDRRALDVTSDKPLVNSLNYLTYLVSSSAKVRETLSIDGGIERLIEILHECHNNNIQDNTNNEKKLLTAWKWTLAFQCLVLAGTRGTEKIRQKVVEAGILPIIATVLDNYLSLNERTFLHANARQLLPQQQQSQQQSQQLAPPQGANPDLPDQSDPTHIPFTNPFPPQMNSQPRQNIREQFAVFNTQVDTNMMQAAAPQVTMDDTINQTNTTNFSNFNMSDDAYANPIQVATAAIERTSISNNNPSAYFNHVSNLTFDDYENLTIEQLFKLIRIANLGPQYANGDQPPPHYKNNTINNDIRRRYIIVNIINKLKQEKQLEMLDDKFFNDCDYEMDSNLQFLSDMYTKDFENNRITKTSSSVRNFTETGVVIPRDDDIVWSLQLLAYISKYPYLKDVLQNTHLVLDMSIREKHARSNLERQMKLKMKKNLAIKLRSSTNNSPKSLKLSCLTFKPSPSNSPQMVNADSLILEDETIKLLNDESTGLTENAEIEDEAESVEADDDDDDDDDDDDDESDIISSASVNTEELLMDDYAKRTIENSDYLVKLYDSIIDCESIVDDLEREISLFQINERMNKFIELESKKLTNIIISTRKERKELLEKKWDYDNYNNFDIDDDGNTEDQDHSLGEYRRVNLFPMVEKFTFLSGTDMYYWSGIIMRNSCRRNDLRGGVRQCGNLECGRWESYPREFSKCRRCKRTKYCSRDCQMRAWHCHRNWCIPSTSSSTSNTTASNAHGGNTDTSAHSAVTTDNDQENQDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARD4</accession>
    <name>G3ARD4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L-serine ammonia-lyase</fullName>
            <ecNumber evidence="5">4.3.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_62338</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-serine = pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:19169"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="EC" id="4.3.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.17" evidence="5"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376519.1">
        <property type="nucleotide sequence ID" value="XM_007376457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARD4"/>
    <dbReference type="FunCoup" id="G3ARD4">
        <property type="interactions" value="502"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARD4"/>
    <dbReference type="GeneID" id="18874279"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62338"/>
    <dbReference type="eggNOG" id="KOG1250">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021152_3_1_1"/>
    <dbReference type="InParanoid" id="G3ARD4"/>
    <dbReference type="OMA" id="RVHFYAS"/>
    <dbReference type="OrthoDB" id="7773036at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000040">
        <property type="entry name" value="L-serine dehydratase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF2">
        <property type="entry name" value="CATABOLIC L-SERINE_THREONINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="11">
        <location>
            <begin position="10"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="331" mass="35751" checksum="B2C70D25969CC459" modified="2011-11-16" version="1">MTMSEQPSIKTTLVEVTDKLQDPPCRIFFKNEYEQPSGSFKLRGMGHLIHESISQAKAQGKSNVRVFSSSGGNAGLAAAYSAKYYGLPCTVVLPDTSKANVIAKLQSFGAEVIIHGKHWGEADSYLKELVAGLDDSEYPVYCHPFDNALLWQGHGNFIDELTEQLSAEDLAKVKGVVCSVGGGGLYNGVVEGLKRNPALCKVPILAIETKQAPTFNEAIKQGKVVHLSQVNTLATSLASPYLSEKSLENHSSHKTHVVEIEDLDAVQGVLDMYDLFGLMVEPACGASVSVAFKRQDLLKSFDVKSDDIVVVIACGGSGVTEEILQQYRSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARF6</accession>
    <name>G3ARF6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_72116</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376055.1">
        <property type="nucleotide sequence ID" value="XM_007375993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARF6"/>
    <dbReference type="STRING" id="619300.G3ARF6"/>
    <dbReference type="GeneID" id="18875415"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72116"/>
    <dbReference type="eggNOG" id="ENOG502QT79">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010476_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARF6"/>
    <dbReference type="OMA" id="NLCHFKV"/>
    <dbReference type="OrthoDB" id="2534600at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051439">
        <property type="entry name" value="XlnR/Xlr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47663">
        <property type="entry name" value="XYLANOLYTIC TRANSCRIPTIONAL ACTIVATOR XLNR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47663:SF1">
        <property type="entry name" value="XYLANOLYTIC TRANSCRIPTIONAL ACTIVATOR XLNR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="91"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="151"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="227"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="795"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="836"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="941"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="173"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="227"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="896"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="947"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="959"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="974"/>
            <end position="984"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="984" mass="110122" checksum="B51C40A77B8FF8E4" modified="2011-11-16" version="1">MIHRTFHPNRSYSSSMLPHLSTTNNLQPPNAHDPAASSVDSNNSPESVISNFFTTGGANTINSSVTKLTNKHTIIHPTNKPAKKKKSNIRACDGCAIRKVKCEATRPCSHCVANNLKCTSLRERKKSGPKNLHRKTLDSINSLSEVIEINQPSRSTSANGGATNGAYTPARPALSASSSVSNVLSKEDSNEGSNDPSAMEITSANSTPSSVSAQQAAAFMSNVSSLAASTQPQMPESTTGPVLPMPNPYSASIPLPTGTAIKQDEPSSAQSSTLASPPMSKSETANSTNSAQEYIVTPHHLVENLNLIGEEPAIFELLKPLTLKSLTTNYSKLVEFLVSNYPNVKNSPYNPFHSEINLIHHHNDPLFLSTLLIILTINLLISEFMIKLKKQKFKDFLQYSKKKLMYRAFKNYRNLCHFKVLEIYTLVEMSFIVPPVIPPRSVGNQSQHQFQHLNQYQIYYNLAVSSFHMCNYFHILNLTNTLNTTNSGQVYNYGNEAQEHEKILYINRAITYFQLINVRNNEPLGPVAELFAALYIAERYYVIYSSHNYNLNLTRNNDIVLRLDSQRMRAFISLPGNDLEHDYVFALTKVVNDYRLIDELCHRSNFNVLLSYDSTSMNFAKFTEVQGLISSIPVHEPIHEIVKSILLFKLLLIFPGDFQQCKEVVVTLVSVLNTVLEQADSDVFKVQMSNHQVCQPLLHLLKIFLEMKQKETRENSQDTRDQEDQNILIRYSDNLIKHFPFFNNINKLVRAHKILNNWFLNLSEYRKRKQQRQQQQQQQQQQDIAMPVATARPAPQQPALFGMSPHPPPLLPSLHSSGSLKDQDVDLAELLKDFHDSNIRSNNSSNRVSQDQTPEEYENAQPQHRAEDEEDEEEEFFSIVPKKQHGPKLALPPPVQQTQSATQQPQMTPGNDVDTPASLHFTASTKNFLSLFAATNFEEAMNNTSGGAPGASSGGNLGNGSSSAPGNNSFFNLYQLSNVESSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARG5</accession>
    <name>G3ARG5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_140758</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376064.1">
        <property type="nucleotide sequence ID" value="XM_007376002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARG5"/>
    <dbReference type="FunCoup" id="G3ARG5">
        <property type="interactions" value="1395"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARG5"/>
    <dbReference type="GeneID" id="18870315"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140758"/>
    <dbReference type="eggNOG" id="KOG1763">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042870_1_0_1"/>
    <dbReference type="InParanoid" id="G3ARG5"/>
    <dbReference type="OMA" id="GREMFYF"/>
    <dbReference type="OrthoDB" id="278280at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032378">
        <property type="entry name" value="ZC3H15/TMA46_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681:SF0">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681">
        <property type="entry name" value="ZINC FINGER-CONTAINING PROTEIN P48ZNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16543">
        <property type="entry name" value="DFRP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="169"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="169"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="300"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="309"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="355"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="380" mass="43097" checksum="AD0F2B0F0134F76E" modified="2011-11-16" version="1">MPPKKKQPQVSDKNKAKSKAKSAEDKTFGMKNKNKSKRVQQQISQIKAGIDGGLAKKKEAEAKRKAEELKAAEQAKKEAAALFGIQPQKVPFGVDPKSILCEFFKQGVCTKGNKCKFSHDPDVGRKVAKKDLYTDSRNAEDDKENDTMDNWDEEKLRKVISSKHGNPKTTTDIVCKYFIDAVENGKYGWFWVCPNGGNECKYRHSLPPGFVLKTKEQKRLERLAAEAEPKITLEEFLELERSKLDRSKFTPITAETFQKWKQEQRKKRDLQRKEDEKKAKRPLTGREIILQKFSDQYYTEEDEGGDTWDLSQFKQNLPETDDENIKDYGDGTNAQEFYQQEAVAESQPEKSTVDAPEAAAEAIPEPNGTATIVTTEGTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARJ3</accession>
    <name>G3ARJ3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">M-phase inducer phosphatase</fullName>
            <ecNumber evidence="2 10">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_61885</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the MPI phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2 10"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376092.1">
        <property type="nucleotide sequence ID" value="XM_007376030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARJ3"/>
    <dbReference type="STRING" id="619300.G3ARJ3"/>
    <dbReference type="GeneID" id="18874095"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61885"/>
    <dbReference type="eggNOG" id="KOG3772">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_413936_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARJ3"/>
    <dbReference type="OMA" id="RRIHSMC"/>
    <dbReference type="OrthoDB" id="26523at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110032">
        <property type="term" value="P:positive regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01530">
        <property type="entry name" value="Cdc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000021">
        <property type="entry name" value="M-phase inducer phosphatase cdc-25.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000751">
        <property type="entry name" value="MPI_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF17">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00716">
        <property type="entry name" value="MPIPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6 10" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="12">
        <location>
            <begin position="166"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="75"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="425"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="8"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="81"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="540" mass="61080" checksum="E172A1FFC1CCB143" modified="2011-11-16" version="1">MPSSDSVSLDDEAYEEEDDDYDDEEDDHHTSSSPLFRRQPFANLSNLVIPVKKSLTTSTKSFTRSINELSKSFLHTHKDESDQESTEEDDTFETINVTSPTPAKPTVRRFHSMCQTSKEFENYQFQSDNSHLKHTNIITSISDTDSLPRIDADQLYHILHGSHKSEFDECIIIDCRFDYEFEGGHIIGAINLSTKQELETKFLSSMSPSNIKRLIVFHCEFSVFRGPIMARHLRKCDRMANSELYPYLTYPDIVILESGYKGFFMKYPYLCHPQGYVEMKDLNHESNCADQLHKVRQDSKLVRAKSFITNNTEITSPTNLHTRSQSFTNTTTTGKVVKRQRSNSKVISSRLSRASTFSFEQSMFTSSTSLLLSPTTQPTSLFDEFLPPSTSFRNTRPTLTINSSTSSFSDNGFFSSNESLTDGYSSSSSPSCDFEKPLPKPPKTTRVSNPTISSFKFPIKRNWPVQPSSSSDISSLISSPLSMQILTPNSCEADSTELIDPINDTPVECTMLKRYRHGRLSSSVGLYSFADLDIDEGDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARY6</accession>
    <name>G3ARY6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_51807</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376613.1">
        <property type="nucleotide sequence ID" value="XM_007376551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARY6"/>
    <dbReference type="GeneID" id="18871597"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51807"/>
    <dbReference type="eggNOG" id="KOG1339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034490_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARY6"/>
    <dbReference type="OMA" id="SKSCLAH"/>
    <dbReference type="OrthoDB" id="28208at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05471">
        <property type="entry name" value="pepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034164">
        <property type="entry name" value="Pepsin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF75">
        <property type="entry name" value="ENDOPEPTIDASE (CTSD), PUTATIVE (AFU_ORTHOLOGUE AFUA_4G07040)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442331" description="Peptidase A1 domain-containing protein" evidence="4">
        <location>
            <begin position="20"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="5">
        <location>
            <begin position="78"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW31835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="460" mass="50227" checksum="E56B41987208DCE0" modified="2011-11-16" version="1">MRITCIVAVLVHFFLITHALLPVKDFRVKGLANEIQGVKSRAVVNSWSEQVLSYSPDVVFTSLNNTQVLYTDDTNSIYYIYLILDDGHGYKQRFPLLLDTGSAVSWIYNQSCTSNSCQNNNVTKFNYNGNLKLGSTFSITYTNDQVDGTYISIAENNINMQLGRVTSDFKVQDFSLGLTSDSPSIFNGFSVSGILGITTDSSDENIVSQMYDSGLINKKVFGLYLTNPGQIYRGNVPAKELGGVVLFGQDVADNTKKFIGSNTVNYVDVVDNNNNYWLINISDTSVVSSQGESSNVISDSRLTIIDSGTTGIVFPFDDAVAIHKQLFGDTGYLNDQNGNFAFLCNYTNENIVFNVGGTKLSVSSSNFKGQAYTDPSLFGYCASKIQGLNNYGHWVLGQAFLKSFYTVFDLDANQIGFGQASLQSFGIEFGNESSSSASSSVLSTTCVETSTKSVLTTITH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARZ8</accession>
    <name>G3ARZ8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">30S ribosomal protein S10, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_56601</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376626.1">
        <property type="nucleotide sequence ID" value="XM_007376564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARZ8"/>
    <dbReference type="FunCoup" id="G3ARZ8">
        <property type="interactions" value="283"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARZ8"/>
    <dbReference type="GeneID" id="18872051"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56601"/>
    <dbReference type="eggNOG" id="KOG3321">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051208_4_0_1"/>
    <dbReference type="InParanoid" id="G3ARZ8"/>
    <dbReference type="OMA" id="KVESWTL"/>
    <dbReference type="OrthoDB" id="366214at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_uS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS10" evidence="4">
        <location>
            <begin position="99"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW31848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="245" mass="28197" checksum="54BD717E315D27B9" modified="2011-11-16" version="1">MLGRIRIGSGFITRSFSTRGALLQKGFVSPNEYVEQVKQEKIEQEQFKQELLSNQIPYAPKTLSSTVIQSEGRPVPLNVELLKYKPLTLPQTHGDQVAVINFRSYEEADIIRAGEFAMRAAYYLGIPTSHLKSLKTEKRLYTVIKAPFVFAKSKQNFHRITFQKRIVAYDANPDVVDLWLSYLNRHKLDTVDYKVELTARESLNYVEDLKQLKDLELPKAYEGIEDPIAVKVQELLKSDDFKKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS58</accession>
    <name>G3AS58_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_52202</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377050.1">
        <property type="nucleotide sequence ID" value="XM_007376988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS58"/>
    <dbReference type="STRING" id="619300.G3AS58"/>
    <dbReference type="GeneID" id="18871640"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52202"/>
    <dbReference type="HOGENOM" id="CLU_314268_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS58"/>
    <dbReference type="OMA" id="DITVEKC"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="747"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="752"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="931" mass="102470" checksum="2FE97DC89EFCE12A" modified="2011-11-16" version="1">MFFSKPTNPKPFIRVLGKLAVLITNSTASNHQIIKDNQQFYLDDTKRSKVKEFFRRFKSFFLRKVQTQIPVIQSTATCMFQFTGEVTVDSFSSEEEVFTLALKPLLKTGSPSTIVSTADVVNECPIVDTFELPVVTADDFVPKYLSHPLVVPAESIAAPISTVVESACALESSEPTEAAPESVPIAVTTDLATEKVPDLSRSNRKKFKKLFKGIRCSIHRTKNYGSNSQPHTFIDETAEVREQSSVVDATVEKCDKAEVKEQSSVVDETAEVKEHSSIVDITVEKCDKAEVENVRSKRQRIKNLFRKSKSNGQPTTLARESPEVKEQPSLVDVTVEVNEQPPVADATAEECDKAEVKEQSPVVDATAEVKEHSSIVDITVEKCDKAEVENVRSKRQRMKNLFRKSKSNGQPSLIDETAEVNEESPVADAIVEECDNAEVKEQYSVVDVTVEKCDKAKVKEQSSVVDETAEVKEQSSVAGVIVEKCDKAEVENVKSKRQRMKNLFRKSKSNGQPSLIDETAEVNEQSPVADAIVEKCDNAEVKEQSSVVNATVEKCDGVETPVVAGYCTEMASVGSGVQVENVRSRRQRMKNLFRKSKSNGQPSLIDETAEVNEQSPVTDAIVEKCDNAEVKEQSSVVDVTVEKCDKAEVENVKSKRQRMKNLFRKSKSNIQPTTLERESPEIKEQPSLVDVTVEECDKAEVKEQSSVVDETAEVKEHSSIVDVTVEKCDKAEVENVKSKRQRMKNLFRKSKSNGQPTTLARESSEVKEHPSIASTITEKKAFQKVSMLSINESSINRTLTIGTSKSVLPAIASAISTLKSTPVVKLPTASAAAASTPVISFGRSGKCASAFDPSKKVYPVSGSLASVDPAKTSKPVIPNFTSGMACTGLSWNTQIGSSKDAMKWRDQNSQLGNKFGAYMEYNYTSTGYNDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASH1</accession>
    <name>G3ASH1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Transcription elongation factor SPT4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_63016</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">The SPT4-SPT5 complex mediates both activation and inhibition of transcription elongation, and plays a role in pre-mRNA processing. This complex seems to be important for the stability of the RNA polymerase II elongation machinery on the chromatin template but not for the inherent ability of this machinery to translocate down the gene.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the SPT4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377122.1">
        <property type="nucleotide sequence ID" value="XM_007377060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASH1"/>
    <dbReference type="FunCoup" id="G3ASH1">
        <property type="interactions" value="446"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASH1"/>
    <dbReference type="GeneID" id="18874554"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63016"/>
    <dbReference type="eggNOG" id="KOG3490">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138052_2_0_1"/>
    <dbReference type="InParanoid" id="G3ASH1"/>
    <dbReference type="OMA" id="FDGMIAV"/>
    <dbReference type="OrthoDB" id="248751at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032044">
        <property type="term" value="C:DSIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031934">
        <property type="term" value="C:mating-type region heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033553">
        <property type="term" value="C:rDNA heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000182">
        <property type="term" value="F:rDNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001208">
        <property type="term" value="P:negative regulation of transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001209">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000232">
        <property type="term" value="P:regulation of rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090262">
        <property type="term" value="P:regulation of transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07973">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009287">
        <property type="entry name" value="Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022800">
        <property type="entry name" value="Spt4/RpoE2_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038510">
        <property type="entry name" value="Spt4_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12882">
        <property type="entry name" value="SUPPRESSOR OF TY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12882:SF1">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06093">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025023">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01389">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0137">Centromere</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="Spt4/RpoE2 zinc finger" evidence="9">
        <location>
            <begin position="6"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR025023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="112" mass="12532" checksum="190D954F77C13237" modified="2011-11-16" version="1">MSSRSERACMLCGIIQPFKKFYSAGCPNCESLLHYQDNEDGQIQDCTSPSFEGTVALGADNKESWVARWLRIDSFAPGLYAVKVNGKLPPDIIRDLEEQNVIYRPRDGSAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASJ8</accession>
    <name>G3ASJ8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_62552</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG8 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376717.1">
        <property type="nucleotide sequence ID" value="XM_007376655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASJ8"/>
    <dbReference type="STRING" id="619300.G3ASJ8"/>
    <dbReference type="GeneID" id="18874373"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62552"/>
    <dbReference type="eggNOG" id="KOG2069">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044223_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASJ8"/>
    <dbReference type="OMA" id="QIIYLCK"/>
    <dbReference type="OrthoDB" id="1661054at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007255">
        <property type="entry name" value="COG8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21311">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21311:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04124">
        <property type="entry name" value="Dor1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="60"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="81"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="452" mass="51440" checksum="3BFB98D2AECE6504" modified="2011-11-16" version="1">MSILLNTLKDGLDPQYQSLLETDPQLLARSQQYLQELLINDDLLSTEAFMASSVSLASDKSTSFKTSNANSVTGSTAELVSPPPPPPPQPQPRRTLIEEIAELDMNHREINRKLSAITDSNRDLIIDISNDLHIVNSQLSHEYQSQLESISKLLESDVLTTQPHAHPMNAIEINNSILENIDSVLDILELPTLCKVCILQGNYQESLEISMLIQALIIRFPKLTIFKQIHAQIELELKLMVKGLIRLLNTNLKQNNILKIFQILNKLDLLGGTSNLVFNDTQQAQKTKFLKIIYLNSRYKFISNEVSSLKPLIKFNKLTYLKRFIETYREYIFNSLSIFNTIFKSTPSNLEDDENNLLINQFIRNLANLLCQELKTHLPEIKPEGDEDSEVAEIDLHNQIDGLILQIIYLCKSLASFHVDFEPIIVTELCHNTNLISEASWANNLAKVKKHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASM2</accession>
    <name>G3ASM2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_142679</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376741.1">
        <property type="nucleotide sequence ID" value="XM_007376679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASM2"/>
    <dbReference type="FunCoup" id="G3ASM2">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASM2"/>
    <dbReference type="GeneID" id="18870471"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142679"/>
    <dbReference type="eggNOG" id="KOG3772">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107716_1_0_1"/>
    <dbReference type="InParanoid" id="G3ASM2"/>
    <dbReference type="OMA" id="RCTNIPC"/>
    <dbReference type="OrthoDB" id="102559at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF38">
        <property type="entry name" value="ARSENICAL-RESISTANCE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="29"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="148" mass="16842" checksum="8A1D29934A2F2451" modified="2011-11-16" version="1">MTTYALSDLKYIRPSTLRTWFRGGSSPHGSGKFAIIDVRDDDYVGGHIKGSCNYPATNFISLLPSLRKSLYEKKVDDVVFHCMLSQARGPSSALKFLRSLNTIDDPELQEFFKNVHVYVLKGGFQRWAREYGEDSSVTEDFAADLWNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASM7</accession>
    <name>G3ASM7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">4-nitrophenylphosphatase</fullName>
            <shortName evidence="2">PNPPase</shortName>
            <ecNumber evidence="2">3.1.3.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_56702</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>4-nitrophenyl phosphate + H2O = 4-nitrophenol + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21664"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57917"/>
            <dbReference type="ChEBI" id="CHEBI:61146"/>
            <dbReference type="EC" id="3.1.3.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5">Divalent metal ions. Mg(2+) is the most effective.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.41" evidence="2"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376746.1">
        <property type="nucleotide sequence ID" value="XM_007376684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASM7"/>
    <dbReference type="GeneID" id="18872060"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56702"/>
    <dbReference type="eggNOG" id="KOG2882">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043473_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASM7"/>
    <dbReference type="OMA" id="WHPAQSI"/>
    <dbReference type="OrthoDB" id="413953at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006349">
        <property type="entry name" value="PGP_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01452">
        <property type="entry name" value="PGP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF46">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000915">
        <property type="entry name" value="PGP-type_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="29"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="62"/>
            <end position="64"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="316" mass="35381" checksum="DA6617AA05C6B0A4" modified="2011-11-16" version="1">MTIKTNPVYVSTKDTAEHILSKYDNFLFDCDGVIWLDNKLIPKVDKFLQLLRAHNKKFIFVSNNSSKSRQVYLEKFAELGIHNISKNEIYPTCYSAALELTKLQIPLGSKIWVLGDEGIERELTEMGYIPIGGTDSRLDSEWQENHPLLTVDPEVKAVVVGSTKKFNYMKIATTLQYLLYKNKSIPFIGTNIDRSYPGPEGIILPAGGSVVNYMAYTADREFINTGKPSSDFLDIILQDQGFKREKSLMVGDTMYTDIKFGNDGQLGDGQGSLLVLSGGTKFTDLAKLLDNRSLEDESLVPSFYAESLTSLVELLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASM9</accession>
    <name>G3ASM9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_155869</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376748.1">
        <property type="nucleotide sequence ID" value="XM_007376686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASM9"/>
    <dbReference type="GeneID" id="18871034"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_155869"/>
    <dbReference type="HOGENOM" id="CLU_2433011_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASM9"/>
    <dbReference type="OrthoDB" id="4026481at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="91" mass="10533" checksum="4DC3FD5515BC091D" modified="2011-11-16" version="1" fragment="single">MLMTKRKFEEDEYFIFSTPPTSSASASATPPPPSTKRVQTSRIHSEAHIRTIQMMMQAQLELQKHEKKQDHQELIMDEDAADEKSTFVQRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT07</accession>
    <name>G3AT07_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">40S ribosomal protein S28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_57066</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS28 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377200.1">
        <property type="nucleotide sequence ID" value="XM_007377138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT07"/>
    <dbReference type="FunCoup" id="G3AT07">
        <property type="interactions" value="932"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AT07"/>
    <dbReference type="GeneID" id="18872092"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57066"/>
    <dbReference type="eggNOG" id="KOG3502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_178987_1_0_1"/>
    <dbReference type="InParanoid" id="G3AT07"/>
    <dbReference type="OMA" id="SCSIIHN"/>
    <dbReference type="OrthoDB" id="10258930at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04457">
        <property type="entry name" value="S1_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000025">
        <property type="entry name" value="40S ribosomal protein S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00292">
        <property type="entry name" value="Ribosomal_eS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000289">
        <property type="entry name" value="Ribosomal_eS28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028626">
        <property type="entry name" value="Ribosomal_eS28_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01200">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00961">
        <property type="entry name" value="RIBOSOMAL_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="67" mass="7543" checksum="EF110C3D576DB541" modified="2011-11-16" version="1">MDAKSDVTLAKVIKVLGRTGSRGGVTQVRVEFLEDSSRTIVRNVKGPVRENDILCLMESEREARRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT11</accession>
    <name>G3AT11_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nonsense-mediated mRNA decay factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_52339</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in nonsense-mediated mRNA decay.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377204.1">
        <property type="nucleotide sequence ID" value="XM_007377142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT11"/>
    <dbReference type="FunCoup" id="G3AT11">
        <property type="interactions" value="185"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AT11"/>
    <dbReference type="GeneID" id="18871662"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52339"/>
    <dbReference type="eggNOG" id="KOG2162">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015710_0_0_1"/>
    <dbReference type="InParanoid" id="G3AT11"/>
    <dbReference type="OMA" id="KFYQRQH"/>
    <dbReference type="OrthoDB" id="69928at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005697">
        <property type="term" value="C:telomerase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018834">
        <property type="entry name" value="DNA/RNA-bd_Est1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019458">
        <property type="entry name" value="Est1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045153">
        <property type="entry name" value="Est1/Ebs1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696:SF37">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY FACTOR EBS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696">
        <property type="entry name" value="SMG-7 SUPPRESSOR WITH MORPHOLOGICAL EFFECT ON GENITALIA PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10374">
        <property type="entry name" value="EST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10373">
        <property type="entry name" value="EST1_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Telomerase activating protein Est1-like N-terminal" evidence="4">
        <location>
            <begin position="68"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/RNA-binding" evidence="3">
        <location>
            <begin position="238"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="613"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="811"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="613"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="827"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="860"/>
            <end position="882"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU369098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW31171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="882" mass="103023" checksum="72CBC21C70B5E730" modified="2011-11-16" version="1">MAQADEILAYKNQLNEFLTRKFPDLSLLIGFNTLLQSKFQSWIIADLNEYYSLMERGQSETVEFNTMKYLETIWNEFHYPTIKFFQHQHQALFQEIQESFKTCQVEGTANQFKVKPVEMRKVNDNFIKFIKQVFEFCVKLLKYFTTKFSNSFIPREFLAHFNLSVSESAAICKDNNFQANVLYLIHRCVLSLGDICRHQTFVELTYVQPATSNKDFFKFRSLANSDKTQAFFPKYRKAIQYYKYCIMLLPALNEPYNHIGMIHNLVDDKYQAIYWFLRSQFTRIPEYKLGLSNLNNVLKKHWFITALVDVVNNNKERQFKIDDSLNIYLVCLMGYFYYPQQYKNGPNLVKKLSFSKIESEFFKSLSSNFSSLIQVGVREENFILTQLTVLFSFTELCKQKSDDVDVHDKVVRFTFRYIEKIFDCLKQLDFEKIKLTNICIILRFILNWLKENKSMYLTFESRKQSVLGICNILNRFIEYNTDKEHMKLMLTQSNRPTRSYYFWEDVHFRDFKLIKYQFKDFKDDALFQVNNINFLIGDYSELLHDKVPTFLSEDKQKLFAGNPKEVEGEIEKYENGLRIHALVSLGKRLLFACKSFDVSLDETSLRFLVKESQEKKSREMKEKKKETNKQTDDKPKDTLKVQEQKPCPVKKHKKSSQTPTPTPEPQTQTTDELSGPPVKVPVSLEEIESFIAKHTHELKSGISAQEGNSEDAQEPELQLHNMVDSLVDDDSKEVINISTGTLNSSTQNIWKHEPAKDIQPKFFNSLAQPIGTQVHQYPANFSQYLYPQMQFMPSQQSIPQSQLQFPYHSTPMGQLQSPTPPVFASYPQSQPTPQQPQGFPQAQAQPMFGTFPIGPESQKSKSPQNNETGNINNGAYSQYFSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT28</accession>
    <name>G3AT28_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">CCAAT-binding factor, subunit A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_142299</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NFYB/HAP3 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376824.1">
        <property type="nucleotide sequence ID" value="XM_007376762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT28"/>
    <dbReference type="FunCoup" id="G3AT28">
        <property type="interactions" value="721"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AT28"/>
    <dbReference type="GeneID" id="18870439"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142299"/>
    <dbReference type="eggNOG" id="KOG0869">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066247_12_3_1"/>
    <dbReference type="InParanoid" id="G3AT28"/>
    <dbReference type="OMA" id="HLHKYRE"/>
    <dbReference type="OrthoDB" id="386949at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22907">
        <property type="entry name" value="HFD_NFYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000110">
        <property type="entry name" value="Nuclear factor Y, subunit B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027113">
        <property type="entry name" value="Transc_fact_NFYB/HAP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064">
        <property type="entry name" value="CCAAT-BINDING TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064:SF9">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00615">
        <property type="entry name" value="CCAATSUBUNTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="8">
        <location>
            <begin position="23"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW30791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="127" mass="14566" checksum="0484904E57C1FFD0" modified="2011-11-16" version="1">MSSPEIDDDWQNNDYEIKEQDRFLPIANVARVMKKALPDRAKLSKESKECIQECVSEFISFITSQAADRCILEKRKTMNGEDILWAMQSLGFENYSEALKIYLAKYREVCITGTCFGVNLVLTIQIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT74</accession>
    <name>G3AT74_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_62704</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ANP1/MMN9/VAN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376870.1">
        <property type="nucleotide sequence ID" value="XM_007376808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT74"/>
    <dbReference type="STRING" id="619300.G3AT74"/>
    <dbReference type="GeneID" id="18874439"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62704"/>
    <dbReference type="eggNOG" id="ENOG502S17G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048297_2_0_1"/>
    <dbReference type="InParanoid" id="G3AT74"/>
    <dbReference type="OMA" id="SCWGMPN"/>
    <dbReference type="OrthoDB" id="204164at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052086">
        <property type="entry name" value="Mannan_Polymerase_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083:SF6">
        <property type="entry name" value="MANNAN POLYMERASE COMPLEXES SUBUNIT MNN9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083">
        <property type="entry name" value="MANNAN POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03452">
        <property type="entry name" value="Anp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442694" evidence="2">
        <location>
            <begin position="32"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="376" mass="42479" checksum="2308EDDC9CFCA1BF" modified="2011-11-16" version="1">MSFARSKRIPALVVVILIAVVTLMVIPYSQGNSSNLKSIGHSITQQLSFSQSQSTYKKIEHQDKAFQVSTVVYEKESSDNNKHSVLILSSIGSGKSYGGDRDFSKFLDVVRSIVNVRDSSKYEFSLGVQCNDENEFSVIKNAIDSVQDSLEPILSRIILVSAPKLEDQIGLSRGNRHNPKLQRLRRRLIAKSRNFLWTTALKNEQYIFFMDADMISLTENMVEYFVESDKDIVVPRVDTAGGIKDYDKNSWRGQRTKPTQKQLDLMDAGKWNDWDYVPHDVNDQIYHFSTFLKKDLVKHKGDLTYKFPLDSVGGAVLFAKSMVFKQGAIFPTSNIIGTTWDRSEGYDGIETEGLCYLAKPLGFSCWGMPNLVATHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATI0</accession>
    <name>G3ATI0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin-like protein ARP6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Actin-like protein arp6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_62853</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Involved in chromosome stability.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the actin family. ARP6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376975.1">
        <property type="nucleotide sequence ID" value="XM_007376913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATI0"/>
    <dbReference type="FunCoup" id="G3ATI0">
        <property type="interactions" value="324"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATI0"/>
    <dbReference type="GeneID" id="18874493"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62853"/>
    <dbReference type="eggNOG" id="KOG0680">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027965_1_1_1"/>
    <dbReference type="InParanoid" id="G3ATI0"/>
    <dbReference type="OMA" id="FFEEYEC"/>
    <dbReference type="OrthoDB" id="6220758at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10210">
        <property type="entry name" value="ASKHA_NBD_Arp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000040">
        <property type="entry name" value="Actin-like protein ARP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW30942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="398" mass="45734" checksum="509A6935AB610874" modified="2011-11-16" version="1">MKQHLIIDNGAYSIKAGYSNRSAPLKVQNSITKTRDGLLHIGNSYLSHTNNYVGLIQKRPHDQGHLTSWETEKPVWDYTFDQLSQDEIDFSKTKLTLTESPFQLPQLSMNTDQIVFEEYGFDEYYRCIPASLVPWSLDMDKVNGDFTLVIDSGFSATWIVPVIYQSVYWQGVRKLPIGGKLLNGLLREIISFRHYDISDDPILVNTIKESTFFLATDFNQTLKNKQKYSCDFILPDFKTTTTGYMRTKEMTLPPDTQVLKLTDERFSIPESYFHPEIVFDNTPLVQSSPFKNITDLIVESIMACPEITRPLLSANIVLVGGSTNIPNFKTRLESELRRELFQDWQVRVSESDIPEEASWRGGVELAGEADVWKEIAVKKKEYFEHGANWCQKRFGFES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATM0</accession>
    <name>G3ATM0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Chromatin structure remodeling complex protein SFH1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_62884</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF5 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377016.1">
        <property type="nucleotide sequence ID" value="XM_007376954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATM0"/>
    <dbReference type="FunCoup" id="G3ATM0">
        <property type="interactions" value="213"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATM0"/>
    <dbReference type="GeneID" id="18874510"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62884"/>
    <dbReference type="eggNOG" id="KOG1649">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014421_4_0_1"/>
    <dbReference type="InParanoid" id="G3ATM0"/>
    <dbReference type="OMA" id="MWNLNES"/>
    <dbReference type="OrthoDB" id="10258327at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033262">
        <property type="term" value="P:regulation of nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006939">
        <property type="entry name" value="SNF5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10019">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04855">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="358" mass="41209" checksum="436DA5C65757A575" modified="2011-11-16" version="1">MSSLSFDTTSFFVQGLASGLSKRLSEENDNSLLLNIAPTGRQAKRIAQQINYSEDYIDDFDFEDTPSANLTNKGSITNQTQANVQKFSPARNTPYMKELDDEQKISELVGKPQILIPIKIALENSNSTHKLVDFFMWNLNESLITPYQFAEIVCNDLELPNAMISQIADSINQQIEEYNFASNLQLPTNNPCNVIIDLAVNLNKQLYQDRFEWDLNQNEVTPEQFAQIVVADVGLSLEFKPAISHALHEIIIRVKKEIIEGTFNNEIHNLHLVRGIIFENGIRIFTETSIQNGNDHWEPLVEVLTSSEIERRENERIRNLRRLKRENMKRDYDDYSGAKRRQVGRRRFDELEGSWKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATV4</accession>
    <name>G3ATV4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_63189</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377301.1">
        <property type="nucleotide sequence ID" value="XM_007377239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATV4"/>
    <dbReference type="GeneID" id="18874633"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63189"/>
    <dbReference type="HOGENOM" id="CLU_2689380_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATV4"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="74" mass="8753" checksum="8FE9EDA6FA946784" modified="2011-11-16" version="1">MSTSLQQLLFLHYQTDRLGEIAFPKLPEFKLLMTRLVSDLTFMLFISKASIVEPIFIFNLEVFKINQYSYFPQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU42</accession>
    <name>G3AU42_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">WD domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_57204</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377389.1">
        <property type="nucleotide sequence ID" value="XM_007377327.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU42"/>
    <dbReference type="STRING" id="619300.G3AU42"/>
    <dbReference type="GeneID" id="18872107"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57204"/>
    <dbReference type="eggNOG" id="KOG0266">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_1_1"/>
    <dbReference type="InParanoid" id="G3AU42"/>
    <dbReference type="OMA" id="CKGHDTA"/>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059122">
        <property type="entry name" value="Beta-prop_WDR5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF1">
        <property type="entry name" value="CANNONBALL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25175">
        <property type="entry name" value="Beta-prop_WDR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="28"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="WDR5-like beta-propeller" evidence="4">
        <location>
            <begin position="33"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="70"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="113"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="155"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="218"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="365" mass="40056" checksum="FB78A8F386DF9A41" modified="2011-11-16" version="1">MGIPSVEHTTQPSIPDLYLLKYSLNQSSSSPISSINSIRISPDGKTFATCSSDTIIRIYDLTTGTLIAELLGHTKGVSDIVYSPINSNIIASCSDDLTIRLWSIKTKKCIKILRKHTYHITTIKFVSKGNILISGSADETITIWDITSGRILTTLAAHSDPVSSLCLTPDNSIIVSASYDGLMRLFDLETSQCLKTLTYNASHGTATASTTDVLNFPISNVEISPNGLYILSSSLDGLVRLWDYMSNKVIKTYQGINGQPVSEHFNCGAKFIIKTETPKIVSGSEKSGILLWDVQTKEIVYQFNNGNDAVLEVDTLDEGRILVSCSRDGIINVFELNKKYIKQDKIATEELNTRDGTPFEKNPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUB9</accession>
    <name>G3AUB9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_63315</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377466.1">
        <property type="nucleotide sequence ID" value="XM_007377404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUB9"/>
    <dbReference type="GeneID" id="18874704"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63315"/>
    <dbReference type="HOGENOM" id="CLU_819318_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUB9"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050636">
        <property type="entry name" value="C2H2-ZF_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772:SF13">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772">
        <property type="entry name" value="ZINC FINGER PROTEIN 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="303"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="26"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW30495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="339" mass="39040" checksum="E7A59120FD22B003" modified="2011-11-16" version="1">MEEILTTTQPSNKSQENLQTKFGELSVKDVDQHTDFEMKENVQEHHHEQTGIDSRNNLESSDHQQVADSTDDGNDQSYAKSATGSSAIGDKAVVLNQVDFFFTRNDQVKLDDALYIGGCRYICANPDPKGFGYVRSYSYKIQEFPGIFILKMINVQPNELEKKKLADGDPKIVMPTCSTCGRWFPKASTCLQHVILHTFCKVQKLEELTQERLDSYWTKTETWFNCIVGDCTEDHDELAQARWHALSHADPGSKIRNLIPVPKPEFFMKKINDLIHCIGCNRVFESSRDFENHYLIHLGDFPFACKVCYKPFNQKSKLVYHFCYECNERKKKQNLEPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUE0</accession>
    <name>G3AUE0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Isoleucine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Isoleucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ILS1</name>
        <name evidence="16" type="ORF">SPAPADRAFT_143054</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Ile) + L-isoleucine + ATP = L-isoleucyl-tRNA(Ile) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="3"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377487.1">
        <property type="nucleotide sequence ID" value="XM_007377425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUE0"/>
    <dbReference type="FunCoup" id="G3AUE0">
        <property type="interactions" value="1176"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUE0"/>
    <dbReference type="GeneID" id="18870514"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143054"/>
    <dbReference type="eggNOG" id="KOG0434">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_1_0_1"/>
    <dbReference type="InParanoid" id="G3AUE0"/>
    <dbReference type="OMA" id="EIIVIHK"/>
    <dbReference type="OrthoDB" id="1706657at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07961">
        <property type="entry name" value="Anticodon_Ia_Ile_ABEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00818">
        <property type="entry name" value="IleRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000004">
        <property type="entry name" value="Isoleucyl-tRNA synthetase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000023">
        <property type="entry name" value="Isoleucyl-tRNA synthetase,cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000050">
        <property type="entry name" value="Isoleucyl-tRNA synthetase,cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02003">
        <property type="entry name" value="Ile_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033709">
        <property type="entry name" value="Anticodon_Ile_ABEc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023586">
        <property type="entry name" value="Ile-tRNA-ligase_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780:SF1">
        <property type="entry name" value="ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780">
        <property type="entry name" value="SOLEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19302">
        <property type="entry name" value="DUF5915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13 16" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="14">
        <location>
            <begin position="22"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="15">
        <location>
            <begin position="697"/>
            <end position="863"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW30516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1085" mass="124459" checksum="F94D0E6345E1EA70" modified="2011-11-16" version="1">MSESAPAPQQGFSFPGEENTVSKLWDDVNAFHRTLEITEDLPPFAFFDGPPFATGTPHYGHILASTVKDIIPRYATMNGYHVERRFGWDTHGLPVEHEIDKKLGITSKHDVYAMGIDKYNAECRSIVMRYADEWKRTIKKLGRWIDMENDYKTLYPEFMESVWWAFKELYKKDAVYRGLKVMPYSTALTTPLSNFESQQNYKDVNDPAITIAFPLVDDEDTVLVAWTTTPWTLPANIALAVNPNFEYVKIYDEEKKKHFILLEGLLSSLYKKPAAAKYKVVEKLKGSALVGLKYKPLFNYFYEEFKDKGFQVIPGDYVTNDSGTGIVHQAPSYGEEDFQAATAHGVISEKKLPPSVVDDTGKMLPNVPDFQGVYFKDADKLIIKKLSEEGRILVNSQAKHSYPFCWRSDTPLMYRTVPAWFVRIGEVIPEMLDNVEKTNWVPSNIKDKRFSNWIANARDWNISRNRYWGTPIPLWVSDDFEEIVCVGSIEELKELSGRDDITDIHRESIDSITIPSKQGKGDLKRIEEVFDCWFESGSMPYASKHYPFENKDKFLNSFPANFISEGLDQTRGWFYTLTVLGTHLFKTAPYQNVIVTGIVLAADGKKMSKRLKNYPDPSIVLERYGADALRLYLINSPVLRAETLKFKEEGVREIISSVMLPWYNSYKFLKDAADIFKKDNGVDFVYDHDLRSENVMDRWLLASIQSLIKFIHEEMNGYRLYTVVPRLLHFIDDLTNWYIRFNRRRIKGYASDDVEDTKKGLNTLAEALLILSRAMAPFTPYLADGIYQRIKGYFKQETLESIVINPKHKDLRSVHFLSYPSVRQELFDSEIELAVSRMQKVIELGRNIREKKMISLKTPLKEIVILHSDKEYLNDIERLKDYISDELNVRTITITSDEAKYGVEYSCVADWPVLGKKLKKDAKRVKEALPKVSSAEVQAFAESGKITVDGIDLVTEDLQVQRGLPQSVCESKGLESRTFQDVLVILDVNSHPELESEGLARELINRIQRLRKKVGLNTTDDVQVEYQLVKDTINLKSVVTDNAELLLKSTKTPIQEFSAAAGDQVIADEEQTINDTVFNLRLLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUG4</accession>
    <name>G3AUG4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Checkpoint forkhead associated with RING domains-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_63092</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DMA1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377221.1">
        <property type="nucleotide sequence ID" value="XM_007377159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUG4"/>
    <dbReference type="FunCoup" id="G3AUG4">
        <property type="interactions" value="154"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUG4"/>
    <dbReference type="GeneID" id="18874591"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63092"/>
    <dbReference type="eggNOG" id="KOG3872">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017542_3_0_1"/>
    <dbReference type="InParanoid" id="G3AUG4"/>
    <dbReference type="OMA" id="YSHEDFH"/>
    <dbReference type="OrthoDB" id="687730at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032186">
        <property type="term" value="P:cellular bud neck septin ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097271">
        <property type="term" value="P:protein localization to bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090337">
        <property type="term" value="P:regulation of formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000921">
        <property type="term" value="P:septin ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000426">
        <property type="entry name" value="DMA1p Ubiquitin-protein ligase (E3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000030">
        <property type="entry name" value="FHA domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15067:SF7">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE DMA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15067">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17123">
        <property type="entry name" value="zf-RING_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FHA" evidence="15">
        <location>
            <begin position="200"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="343"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="106"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="32"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW30250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="399" mass="44426" checksum="815C9E418B698EF5" modified="2011-11-16" version="1" fragment="single">MSTPIATTTANRVVSLPTKTNSNKLTKSSSNDNVNNITPSPQGNRFGLNFLSGFGMRSHSLSSTERNGGVAEMANSPTISNLNGYSHEDFHPVNGGAIHLATNEEVAEENSDNDGDHEEEGGDEEPQSPRRRRQDTLFDPNTTSSGTDAINLEDTLDKNSPMDKDGFYSVRLTPLIDHSSSSSGLYFPPIIRKIKPDSSISIGRYTEKNKAAAHAPQGSSAPIVFKSKVVSRTHALFQCNGDGQWFLKDCKSSSGTFLNHIRLSPASQESTLMPLIDGDIIQLGMDYRGGTEEVYRCVKMRCEFNKSWQRKVNQFNLEIHKKLKSLHLNDETVIEEGKDMTECVICLMKIEPCQALFISPCSHTWHYKCIRPIIIKSYPQFYCPNCRTMCDLETDLDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUQ7</accession>
    <name>G3AUQ7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_52681</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377584.1">
        <property type="nucleotide sequence ID" value="XM_007377522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUQ7"/>
    <dbReference type="STRING" id="619300.G3AUQ7"/>
    <dbReference type="GeneID" id="18871706"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52681"/>
    <dbReference type="eggNOG" id="ENOG502RQ18">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_990446_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUQ7"/>
    <dbReference type="OrthoDB" id="4093337at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442384" evidence="1">
        <location>
            <begin position="22"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="277" mass="30394" checksum="2BC2AD3D9C89F0DC" modified="2011-11-16" version="1">MKVLCSLISFFFATFVVTAAAIKGDAFQQEFNELLTVLESYNDTSVLKRDYAPLTSALTALNKSGQGVQIAHTMYSSKATQSTVINVVEDYIKATTLTQILKDSDDSDMSVDIVMILFTNSNMIPGLTKIIKTLHDNGVLSFSLKKRGLLDGILGKLNNIFTSIQNESIQTIVKLMNAVTDSEDIYESLEKSGLFISLFEDIVTTTDGQAFSVNLVTDIINKDVITWSSLMSAVNSSAIIANTTSKLFTNATNRQIIFHWISTNGLSVLLEILKALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV22</accession>
    <name>G3AV22_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcriptional protein SWT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_143967</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SWT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377862.1">
        <property type="nucleotide sequence ID" value="XM_007377800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV22"/>
    <dbReference type="FunCoup" id="G3AV22">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="GeneID" id="18870586"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143967"/>
    <dbReference type="eggNOG" id="KOG4689">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048317_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV22"/>
    <dbReference type="OMA" id="WANDWIY"/>
    <dbReference type="OrthoDB" id="2017974at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071032">
        <property type="term" value="P:nuclear mRNA surveillance of mRNP export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18727">
        <property type="entry name" value="PIN_Swt1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000045">
        <property type="entry name" value="Transcriptional protein swt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049014">
        <property type="entry name" value="SWT1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052626">
        <property type="entry name" value="SWT1_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16161">
        <property type="entry name" value="TRANSCRIPTIONAL PROTEIN SWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16161:SF0">
        <property type="entry name" value="TRANSCRIPTIONAL PROTEIN SWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21693">
        <property type="entry name" value="SWT1_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="PIN" evidence="7">
        <location>
            <begin position="120"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="463" mass="52512" checksum="7867A1824F9AF734" modified="2011-11-16" version="1">MSSSSSSLPSRYAPEGLSEEDQHRVITSGHARRSKPIEYTIKTIESKIHDALTNQTYQNNTVDGDIEMVPMDDAEVDVISSYVSYSRDSGNLQVNSGDEMYDDIMMVEKPEETHANGNLSMLVVDTNFILSHLKVVDELKGLAREFGLRIVIPVMVMRELDGLKNSSRLVDDDSPERLSGVSVGHLARWANDWIYNSLANNSSVVIGQKLGQRLDKNAIQDDAILDCCLYFKQRANLVVLLSNDKNLCNKALTNDVLTVTFKTGMSAKLIAKVIYDENVNQFGKLAPMEIEHVPSLKHKHKEASAEPSREWVQSTQEVQAFRSFAEVAEKVYSEIQMILLSAIHHCMEVEYGEDLDLLRDYTKESIVTIQDCSQVLIRFWFTVFDQYFRASPAKFVPFQETGTGRKSVKHPLYVDIPTTPAELVEFIEFWTTALQIIYDGVMDETQQMALEHLIKRWHKMADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVC2</accession>
    <name>G3AVC2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flo11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_68962</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377907.1">
        <property type="nucleotide sequence ID" value="XM_007377845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVC2"/>
    <dbReference type="GeneID" id="18875251"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68962"/>
    <dbReference type="HOGENOM" id="CLU_382690_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVC2"/>
    <dbReference type="OrthoDB" id="4028176at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018789">
        <property type="entry name" value="Flo11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10182">
        <property type="entry name" value="Flo11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51824">
        <property type="entry name" value="FLO11"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442723" description="Flo11 domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="Flo11" evidence="3">
        <location>
            <begin position="38"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Flo11" evidence="3">
        <location>
            <begin position="247"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Flo11" evidence="3">
        <location>
            <begin position="451"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="411"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="609"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="618"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="646"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="700"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="714"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="723" mass="82026" checksum="4E922F646A354D9C" modified="2011-11-16" version="1">MRQTLCFCYLLFQMLLMVAADTGPVTVSRPYTDLPEPSSTYPPSWNGDSICDSPFGALSKANNQPELKIFEFKSIKWIEDNFYEVMIEFEIDGNSYTESELRAIYIFSLQTPDYYLGSVELFVESWEQNLLGDSPFHFYFTWVMEAEDIDQLTCTTPFQVYYDWDTYFATYQHGCFSDELTDLPAQCWDKPNFAETSSQQNLETSSQQNLEASTESFETTQSQGTSNVVATPSTIEPTRTDLPVLPSPIDCPSSWTSNSVCAYDVGEHARNANYPEVQIFEFKSIQYLTGNLYQVLFEFKVDGSVYPKDHLKHIYVGNLHMPGGYSFGDYLYNPQQDDGIDLVGDSPFHFYYSWVMEMQQINEFTCTTPFEISYLWQEVGATYAHGCDVFDGVGDLPLLCWEEVCEPLAQTTTEEEDVSTSAKPESSTVYQEPTPSTINPPRTNLPSMPDPTACPTSWNGNSVCDIPIGAHALSESTYPKVKTFNFESIKFIQDDLYEVTLEFEIEDTIPKSELHAVFAYYLQTPDDYLSSIQLDAYVGNSPYHFYLVWIMKTQNIDQYICTTPFTIEYNWNGIPFSYTHGCLPDDFGDLHDSPLQCWKNVCDIHEQTTTEQESNAYTESPTSQTQEETTISTTNETTIATTSNPQDETTIATTSNPQDETTIVASSNTQDETTLATTSNTQYETSSETTDETTSKMTSVSSTEKQEVTSTKVRYCRAPRRKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVK4</accession>
    <name>G3AVK4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DBF4-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_73393</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377719.1">
        <property type="nucleotide sequence ID" value="XM_007377657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVK4"/>
    <dbReference type="FunCoup" id="G3AVK4">
        <property type="interactions" value="171"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVK4"/>
    <dbReference type="GeneID" id="18875482"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_73393"/>
    <dbReference type="eggNOG" id="KOG4139">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023948_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVK4"/>
    <dbReference type="OMA" id="GMKIWAI"/>
    <dbReference type="OrthoDB" id="21380at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031431">
        <property type="term" value="C:Dbf4-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010571">
        <property type="term" value="P:positive regulation of nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901987">
        <property type="term" value="P:regulation of cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3410:FF:000001">
        <property type="entry name" value="Protein DBF4 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3410">
        <property type="entry name" value="DBF zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055116">
        <property type="entry name" value="DBF4_BRCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013939">
        <property type="entry name" value="Regulatory_Dfp1/Him1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051590">
        <property type="entry name" value="Replication_Regulatory_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006572">
        <property type="entry name" value="Znf_DBF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038545">
        <property type="entry name" value="Znf_DBF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15375">
        <property type="entry name" value="ACTIVATOR OF S-PHASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15375:SF26">
        <property type="entry name" value="PROTEIN CHIFFON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22437">
        <property type="entry name" value="DBF4_BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08630">
        <property type="entry name" value="Dfp1_Him1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07535">
        <property type="entry name" value="zf-DBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00586">
        <property type="entry name" value="ZnF_DBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51265">
        <property type="entry name" value="ZF_DBF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DBF4-type" evidence="6">
        <location>
            <begin position="459"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="442"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW29953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="521" mass="59282" checksum="B934B694A0D807BE" modified="2011-11-16" version="1">MDNDNTGNNPSAATTAMPGRNLKKQFNSLLTKPRRPLRETSSNIPSPARRLAGEELYNWQQSWRKIMRESTVYFEDVQELGRMQTTEYRRASKLLKLVGCAITPFYDNTVSIIVSRRHYDAKKTYPSNDIFSNVASLKIKVWNYEKVFRFLKNLGVNILTGVDENALNTHTILPPSLTTDSTSKTNRDNLYNLLKEEKIYGSTDRDPNAKRDDLHYLGKNYLFVYDLLQKVRPIAVREWHDDHSYTTLNLTMDGKCPFIFDPSENSNTERKRLRRARKFEASQEYRESLKQATYNIINNIKNGGVSLHVSGFSGTSTSTDKMIHEEDFIPQDQNTTVIQNSNSTSSRKSSVEWTSDAKPLKFRQPIPPALARNSSCVQSITTTGKTFDVFAASGYNSASNAVQFSMDSNLNSNAAAMVGNGLGPMMSQVPSRNLNNLKRRIIMKKQQRKTSPPKSKDVRESNPGYCENCRAKYDCFEDHIKSNRHRNFACDDSNFKDIDELITKLNESRSFGFVASSGDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVV9</accession>
    <name>G3AVV9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Nicotinate phosphoribosyltransferase</fullName>
            <ecNumber evidence="3 9">6.3.4.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_63630</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate at the expense of ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + nicotinate + ATP + H2O = nicotinate beta-D-ribonucleotide + ADP + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.</text>
    </comment>
    <comment type="PTM">
        <text evidence="9">Transiently phosphorylated on a His residue during the reaction cycle. Phosphorylation strongly increases the affinity for substrates and increases the rate of nicotinate D-ribonucleotide production. Dephosphorylation regenerates the low-affinity form of the enzyme, leading to product release.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the NAPRTase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.21" evidence="3 9"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377770.1">
        <property type="nucleotide sequence ID" value="XM_007377708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVV9"/>
    <dbReference type="FunCoup" id="G3AVV9">
        <property type="interactions" value="195"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVV9"/>
    <dbReference type="GeneID" id="18874839"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63630"/>
    <dbReference type="eggNOG" id="KOG2511">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030991_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVV9"/>
    <dbReference type="OMA" id="IEHCLEY"/>
    <dbReference type="OrthoDB" id="193380at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00457"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004516">
        <property type="term" value="F:nicotinate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034355">
        <property type="term" value="P:NAD+ biosynthetic process via the salvage pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019358">
        <property type="term" value="P:nicotinate nucleotide salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.140.10:FF:000009">
        <property type="entry name" value="Nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.140.10">
        <property type="entry name" value="nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00570">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041525">
        <property type="entry name" value="N/Namide_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040727">
        <property type="entry name" value="NAPRTase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006406">
        <property type="entry name" value="Nic_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007229">
        <property type="entry name" value="Nic_PRibTrfase-Fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01514">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003704">
        <property type="entry name" value="PRK05321.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098:SF1">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04095">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17767">
        <property type="entry name" value="NAPRTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000484">
        <property type="entry name" value="NAPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6 9" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nicotinate phosphoribosyltransferase N-terminal" evidence="11">
        <location>
            <begin position="9"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Nicotinate/nicotinamide phosphoribosyltransferase" evidence="10">
        <location>
            <begin position="162"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW30004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="417" mass="47936" checksum="7CBB878632CCDC1D" modified="2011-11-16" version="1">MEPIITSLLDTDLYKLFMHAAVHKHFPNVPVIYKYTNRTPQLKLNKDAIQWVKHQIQSLENLRFTNEEIEFVKKTLPQFPAEYFEYLQTFKFKPEEQIKYLDGDDDLKLEVAGNWQDTILYEIPLLALISEAYFKFVDTDWSYDGQYEQALDKVRQLVGNKCAFSEFGTRRRRSLKAQEIVVQAMSDFRKENPELGAKYIAGTSNVHLAMKYSFMPTGTVAHEWYMGIASITQNYPEANRIAMDYWIDTFGKENAGLALTDTFGTDNYLSVFKPPYSDAYVGVRQDSGDPEVFAEKVAAHYAKLNYPKNSKVICFSDSLDIEKCLKYRKKADELGLLTTFGVGTFFTNDFISTTTGKKSAPLNIVIKLREANGQFAIKISDNIGKNMGDPATVKRVKQELGYIEKEWEEGDETHRWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADZ1</accession>
    <name>G3ADZ1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_57778</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372127.1">
        <property type="nucleotide sequence ID" value="XM_007372065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ADZ1"/>
    <dbReference type="STRING" id="619300.G3ADZ1"/>
    <dbReference type="GeneID" id="18872193"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57778"/>
    <dbReference type="eggNOG" id="KOG3986">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_960317_0_0_1"/>
    <dbReference type="InParanoid" id="G3ADZ1"/>
    <dbReference type="OrthoDB" id="1881at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2440">
        <property type="entry name" value="Carbohydrate binding type-21 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038175">
        <property type="entry name" value="CBM21_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="290" mass="32913" checksum="0F575E7A50B183B8" modified="2011-11-16" version="1">MCIKYYANASEYWDNNNFKNYEIKLIKTVREITPGANIEQHKAKPKYSNSYLKRVASDSQIELHKNEIMNRLNDEVEEAKRKEQEKEEKGEGGSESEDEDEEGLDDAKSSALFINDNSSDLNDFVKNNYYLSSPLLSSLYKNPDGNEEYLKNSEDAKIISPSPQPSIPSSKFRAKFQNQLSESQKQLDTSEFNRFGFNWVDEEQQKQNEATSNPSKPQRTSPLSRNKFMNSKSYKELLDNYCFFSSSNDENHSPDAELPYPNTSINGNGNGKPSSNGGGNSYTVSSFLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE67</accession>
    <name>G3AE67_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="4 14">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_131790</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4 14"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373013.1">
        <property type="nucleotide sequence ID" value="XM_007372951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE67"/>
    <dbReference type="FunCoup" id="G3AE67">
        <property type="interactions" value="972"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AE67"/>
    <dbReference type="GeneID" id="18869610"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131790"/>
    <dbReference type="eggNOG" id="KOG3359">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008438_5_0_1"/>
    <dbReference type="InParanoid" id="G3AE67"/>
    <dbReference type="OMA" id="GSPFNTW"/>
    <dbReference type="OrthoDB" id="292747at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF52">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="243"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="639"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="677"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="703"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="733"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="347"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="430"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="501"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW35601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="777" mass="89012" checksum="4C8AE2534BC3D0D2" modified="2011-11-16" version="1">MSDSFSSGASYKTGESQLTRRYQTDSSKDDTQVIAKDGDLEEIIARTSNLNINKTHHKKSVSTLFTKLINPFILTALSAFVRLYRIEVANKVVWDEAHFGKFGSHYLKHEFYFDVHPPLGKLLVGLSGYLAGYDGSFDFESSVDYPEDMNYVLMRIFNCVFGIVCTPLAYKTAVLLGYNQLTCWFISLSVIFEMLSLTLSKFILLDSILLFFTVFAYFALIHVHNLRTSDQLLTFQGIKWLSITGFAIGCVCSTKWVGLFMTALVGLYTILDLIIKFYQTISKQLPWKRYLLHWITRIFTLIVLPVTIYLAAFKVHFLVLSHAGEGDSAVSTLLQASHEGSDIKSGPRSVAYGSFVTLRSQGLSQNLLHSHPHNYPEGLQEQQVTTYAYKDENNVFLIEFDINAAISGKRALPETSGNNGTETETTIESQQLLQNGDAIRLFHNSTGRYMHANAIPAAVAKTHFEVSCFGTIDTTEIKDEWILEIQTQEESPSQFFANESGHELHPISTNFRLRNKELGCYLATTGYAYPSWGFSQGEVVCKYSYFNRDKSTWWNVEEHVNDKLPLPSETYVPPKPKFWREFVLLNFAMMTSNNALIPDPDRFDKLSSEWWEWPILKTGLRMCSWGADDIKFFLIGNPFVTWFSSLSLILFLFYLLSVGFKYQRQTVRYEVDSWESLLSQGILPFIGWFLHYFPFIIMGRVTYLHHYVPALYFAIFTMGFVVDRFATVVPKKISYVIYGLLYIAVIGSFWYLRVLAFGMEQSSKQYKHLKILDSWMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE88</accession>
    <name>G3AE88_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58836</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373034.1">
        <property type="nucleotide sequence ID" value="XM_007372972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE88"/>
    <dbReference type="STRING" id="619300.G3AE88"/>
    <dbReference type="GeneID" id="18872635"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58836"/>
    <dbReference type="eggNOG" id="ENOG502T0I1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1437755_0_0_1"/>
    <dbReference type="InParanoid" id="G3AE88"/>
    <dbReference type="OrthoDB" id="27975at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="189" mass="21302" checksum="99B0054C7252D526" modified="2011-11-16" version="1" fragment="single">PPTLPPPPNYQPYVKVIDSPYTSRFIAPVFNCPTYSCPICDTKFMLSGLTISDSYNYFVKSTPKETERIELIGMEKYRLIDDSITAHIPPEDDEDMIVLSSDDEEDQRIKKEEEAAAAAAEAARIFRIATLPPQVRPLYNAVYNNYPDNVLIGRTDLLDDEEVWGTPGNNSGDYYSHGSSWEDPVVLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AER5</accession>
    <name>G3AER5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cytosolic Fe-S cluster assembling factor NBP35</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">NBP35</name>
        <name evidence="12" type="ORF">SPAPADRAFT_58896</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NBP35-CFD1 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373103.1">
        <property type="nucleotide sequence ID" value="XM_007373041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AER5"/>
    <dbReference type="FunCoup" id="G3AER5">
        <property type="interactions" value="588"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AER5"/>
    <dbReference type="GeneID" id="18872665"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58896"/>
    <dbReference type="eggNOG" id="KOG3022">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024839_0_1_1"/>
    <dbReference type="InParanoid" id="G3AER5"/>
    <dbReference type="OMA" id="VSGCPMR"/>
    <dbReference type="OrthoDB" id="1741334at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904564">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly scaffold complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000427">
        <property type="entry name" value="Cytosolic Fe-S cluster assembly factor NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03038">
        <property type="entry name" value="NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028601">
        <property type="entry name" value="NUBP1/Nbp35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264:SF35">
        <property type="entry name" value="CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="73"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>ligand shared with heterodimeric partner</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>ligand shared with heterodimeric partner</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW35691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="329" mass="35297" checksum="1E87B5922C11579A" modified="2011-11-16" version="1">MSPSIEPTQELNAPEPEHCPGPESDLAGKEDACQGCPNQEICSSNTVKGPDPDLPLINARLSQIDHKILVLSGKGGVGKSTFTSMLSWALAADDSLEVGAMDLDICGPSLPRMLGAEGEGVHQSNSGWSPVYVADNLGLMSIGFMLPDPDSAIIWRGAKKNGLIKQFLKDVNWGEHLDYLVVDTPPGTSDEHLAVTTYMKEVGVDGALIVTTPQEVALLDVRKEIDFCRKAGIKILGLVENMSGFVCPNCKGQSQIFKPTTGGGEQLCKDLNLKFLGSVPLDPRIGACCDHGRCFFDEYPDSPATTAILDVVDALRDQVELDMTKLTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AES0</accession>
    <name>G3AES0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">methylmalonate-semialdehyde dehydrogenase (CoA acylating)</fullName>
            <ecNumber evidence="2">1.2.1.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_58899</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.27" evidence="2"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373108.1">
        <property type="nucleotide sequence ID" value="XM_007373046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AES0"/>
    <dbReference type="STRING" id="619300.G3AES0"/>
    <dbReference type="GeneID" id="18872668"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58899"/>
    <dbReference type="eggNOG" id="KOG2449">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_10_1"/>
    <dbReference type="InParanoid" id="G3AES0"/>
    <dbReference type="OMA" id="GGAKNHI"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004491">
        <property type="term" value="F:methylmalonate-semialdehyde dehydrogenase (acylating, NAD) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006210">
        <property type="term" value="P:thymine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07085">
        <property type="entry name" value="ALDH_F6_MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000002">
        <property type="entry name" value="Methylmalonate-semialdehyde dehydrogenase (Acylating)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000003">
        <property type="entry name" value="Methylmalonate-semialdehyde dehydrogenase [acylating]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010061">
        <property type="entry name" value="MeMal-semiAld_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01722">
        <property type="entry name" value="MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866">
        <property type="entry name" value="MALONATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866:SF3">
        <property type="entry name" value="METHYLMALONATE-SEMIALDEHYDE DEHYDROGENASE [ACYLATING], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="5">
        <location>
            <begin position="55"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="544" mass="58914" checksum="A875D91E24228FE7" modified="2011-11-16" version="1">MLSRVVRNKAAIRAFQINIMRFKTTIALSSTTSSYPTTHVKDQPYDTPSFVNNEFIKSESSEWFDIHDPATNNVVSRVPQSTPQELEEAIASAHNAFPSWRDTSVIKRQGIAFKFVQLLKENMDRIASVIVLEQGKTFVDAQGDVTRGLQVAEAACNITNDLKGETLEVSTDMETKMIREPLGVVGSICPFNFPAMVPLWSLPLILVTGNTAVIKPSERVPGAAMIICELAAKAGVPPGVLNVVHGKHDTVNKLIEDPRIKALTFVGGDKAGKYIYEKGTSLGKRVQANLGAKNHLVVLPDANKQQFVNAINGAAFGAAGQRCMAISVLVTVGKTREWIDDIVKDAKQLVTGSGFDPKSDLGPLINPSSLTKAESIIEDSIKAGAKIVLDGRGYRPKDSQFANGNFLAPTILTGVKPGMRAYDEEIFAPVLSVINVDTIDEAIEIINTNKYGNGTSLFTNNGGSAQYFTKRIDVGQVGINVPIPVPLPMFSFTGSRGSFLGDLNFYGKAGITFLTKPKTITSLWKSNLVDEEILKPSTSMPVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEZ7</accession>
    <name>G3AEZ7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Autophagy-related protein 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_131523</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Autophagy-specific protein that functions in response to autophagy-inducing signals as a scaffold to recruit other ATG proteins to organize preautophagosomal structure (PAS) formation. Modulates the timing and magnitude of the autophagy response, such as the size of the sequestering vesicles. Plays particularly a role in pexophagy and nucleophagy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Preautophagosomal structure membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ATG17 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372213.1">
        <property type="nucleotide sequence ID" value="XM_007372151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEZ7"/>
    <dbReference type="FunCoup" id="G3AEZ7">
        <property type="interactions" value="155"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEZ7"/>
    <dbReference type="GeneID" id="18869584"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131523"/>
    <dbReference type="eggNOG" id="ENOG502RW77">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_565132_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEZ7"/>
    <dbReference type="OMA" id="TNHFDQC"/>
    <dbReference type="OrthoDB" id="1937984at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007240">
        <property type="entry name" value="Atg17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045326">
        <property type="entry name" value="ATG17-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28005">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28005:SF1">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04108">
        <property type="entry name" value="ATG17_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Autophagy protein ATG17-like" evidence="7">
        <location>
            <begin position="18"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="459" mass="53359" checksum="C73D666B140C5DF1" modified="2011-11-16" version="1">MTISQHEVLRWSTEAQHTLERAQKICTSAQASLHSTQNELSVHLPDIMDAVEFLYDALARQHETVLRITESLKRHVRTKVDSVFEEFGSVLDPAVEKLDGIISQLRSTTVPLFLLREKEEEEQEDGKSLADFIAMDSIALLKQNINIYKSNCGRLRKALLGNFEDILEYNMKMNKQFSQIKKRYENLVPLKVEIKSIQSKESTIGTVLRENQALENELVSILEMLTNHYDQCMKAVELISKGNTTGINLEVLQGDVRELPDVAKELNAVYEIILKNEERSQKYLHTETIEATITLLQQELQTYRTFKTTALPKFILLLSECENKLQISSIPSTAETPTRLYVQILEELTFHYTQFLHIYKTKYLSELYHEQYTYPRKFLKKLTELLNQDMYRIQLEETERRKQWTAKYGNFIPKEFNLELELPSIVQIITEGLENIQSGEAEFNEGKEKELLDLIKLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF25</accession>
    <name>G3AF25_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">High affinity glucose transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">HGT1.2</name>
        <name evidence="10" type="ORF">SPAPADRAFT_64051</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372241.1">
        <property type="nucleotide sequence ID" value="XM_007372179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF25"/>
    <dbReference type="STRING" id="619300.G3AF25"/>
    <dbReference type="GeneID" id="18874955"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64051"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_12_1"/>
    <dbReference type="InParanoid" id="G3AF25"/>
    <dbReference type="OMA" id="EQSWEIG"/>
    <dbReference type="OrthoDB" id="4142200at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000026">
        <property type="entry name" value="MFS quinate transporter QutD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF7">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="345"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="390"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="462"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="33"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="546" mass="61020" checksum="5253CFA6A50EA10E" modified="2011-11-16" version="1">MILSRIDQALFDPAIKVRNFLDRFPKIYNIYMIAMCSTIAGMMFGFDISSMSAFIGTDSYINFFDNPGSTIQGFITSCMALGSFFGSIASSFVSEPFGRRFSLLVCSFFWMVGAAIQSSSQNRAQLIIGRLISGIGVGFGSSVAPIYGAELAPRKIRGLIGGLFQFSVTLGILIMFYISYGLGKIDGVASFRIAWGLQIIPGLILFTACFFIPESPRWLAKRDFWDDTEYIIAKIQAHGDKDDPDVLIEIGEIKDQLLVEESANSIGYATLFTRKYFRRTFTAISSQIWQQLTGMNVMMYYIVYVFQMAGFTGDTNLIASSIQYVLNVVMTIPALLLLDRVGRRPLLIVGAICMMAFQFGVAGTLAVHSVPWPDSGNESVNIQIPDTKMYASRGVIACCYLFVCSFASTWGVGIWVYCAEIWGDSRISQRGNALSTAANWIFNFAIGMYTPSGFKNISWKTYIIYGVFCFFMTVHVFFGFPETRGKRWEEIGQMWDENVPAWQSRSWTPIIPINPETEKAARISLEREEKRRASSVVRPSISDPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF70</accession>
    <name>G3AF70_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Alpha-1,6-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_132521</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 34 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372271.1">
        <property type="nucleotide sequence ID" value="XM_007372209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF70"/>
    <dbReference type="FunCoup" id="G3AF70">
        <property type="interactions" value="109"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF70"/>
    <dbReference type="GeneID" id="18869666"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132521"/>
    <dbReference type="eggNOG" id="KOG4748">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021434_1_0_1"/>
    <dbReference type="InParanoid" id="G3AF70"/>
    <dbReference type="OMA" id="VENACNQ"/>
    <dbReference type="OrthoDB" id="205108at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008630">
        <property type="entry name" value="Glyco_trans_34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306:SF10">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05637">
        <property type="entry name" value="Glyco_transf_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="432" mass="49939" checksum="6DC9502648880BB1" modified="2011-11-16" version="1">MFGQTGGSKFKPRQRQKSILPLPATFNRIHTLQRKNIPWIALLILFMWYFFNPFGVIAGMFRSATPNSYPPPHPLTSHNVIETNSRFIYPALELSPYLKGLPLDRIMKVSRVRDSNFPEIEKTLIKSLNSLDDPDVETQRAKEEAENAVSDVSKAINSFKNQDKVVFKPKNPDDYPQVIIVTAVDYERYSLDGMTKLIQNRVDYAHFQNYGTFVRYSQEFLPQLNAQSFLQTREKAKWVRIYCMQAARFAFPKAKWFWFVDQDSLIMDMDINIEQYMLNDDALDPIMMREQPLVPPSGAIKTYKNVRANSIKLIVTQSESMIETTSFVLRNDHVAKSILEIWGNQLYLNYQSFPYGPDSAITHILQWHPFILSKTTIVPAKTIAAAHNFKKKEDSKFTYSEGDFVVQWGDCKAPACEQILNQYDMLVKKPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF72</accession>
    <name>G3AF72_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1,3-beta-glucan synthase</fullName>
            <ecNumber evidence="3">2.4.1.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">1,3-beta-D-glucan-UDP glucosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">GLS1</name>
        <name evidence="14" type="ORF">SPAPADRAFT_145419</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;3)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;3)-beta-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21476"/>
            <dbReference type="Rhea" id="RHEA-COMP:11146"/>
            <dbReference type="Rhea" id="RHEA-COMP:14303"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37671"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.34"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 48 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.34" evidence="3"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372273.1">
        <property type="nucleotide sequence ID" value="XM_007372211.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AF72">
        <property type="interactions" value="611"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF72"/>
    <dbReference type="GeneID" id="18870687"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_145419"/>
    <dbReference type="eggNOG" id="KOG0916">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000844_0_1_1"/>
    <dbReference type="InParanoid" id="G3AF72"/>
    <dbReference type="OMA" id="AWTDFFI"/>
    <dbReference type="OrthoDB" id="1880850at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000148">
        <property type="term" value="C:1,3-beta-D-glucan synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003843">
        <property type="term" value="F:1,3-beta-D-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006075">
        <property type="term" value="P:(1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051278">
        <property type="term" value="P:fungal-type cell wall polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026899">
        <property type="entry name" value="FKS1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056261">
        <property type="entry name" value="FKS1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003440">
        <property type="entry name" value="Glyco_trans_48_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741:SF48">
        <property type="entry name" value="1,3-BETA-GLUCAN SYNTHASE COMPONENT FKS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741">
        <property type="entry name" value="LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14288">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23605">
        <property type="entry name" value="FKS1_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02364">
        <property type="entry name" value="Glucan_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01205">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="488"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="528"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="558"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="618"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1299"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1355"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1442"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1468"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1558"/>
            <end position="1579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1605"/>
            <end position="1632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1644"/>
            <end position="1668"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1674"/>
            <end position="1692"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1806"/>
            <end position="1825"/>
        </location>
    </feature>
    <feature type="domain" description="1,3-beta-glucan synthase component FKS1-like" evidence="13">
        <location>
            <begin position="295"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="106"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="27"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1888" mass="217115" checksum="067B03AA581C188C" modified="2011-11-16" version="1">MSYNDNNHNYYDPNQQPSGAPLQGDGYYQQPYDDMNQQQQDYYDPNVQYQQQPYDMDGYQDGAQYGSQPAQGYNADPEAFSDFSYGGQTPGASYDQYGTQYTPSQMSYGGDPRSSGASTPIYGGQGQGYDPSQFQMSSNLPYPAWSADPQAPIKVEHIEDIFIDLTNKFGFQRDSMRNMFDYFMTLLDSRSSRMSPAQALLSLHADYIGGENANYRKWFFSSQQDLDDSLGFANMALGKIGRKARKASKKSKKARKAAEEHGEDVDALATELEGDYSLEAAEIRWKAKMNTLTPEERVRDIALYVLLWGEANQVRFTPECLCYIYKTASDYLNSPLCQQRQEPVPEGDYLNRVITPLYRFLRSQVYEIYEGKFVKREKDHNKVIGYDDVNQLFWYPEGISRIMFEDGTRMVDIPQEERYLRLGEVEWNNVFFKTYKEIRTWLHFITNFNRIWIIHASIYWMYTAYNSPTLYTKNYVQTRNQQPLASSRWAACAIGGVVASLIQIFATLFEWMFVPREWAGAQHLTRRLMFLILIFFVNLAPVVYTFKITGLTLYSKSAYAVSIVGFFIAVATIIFFAVMPLGGLFTSYMNKRSRRYISSQTFTANFIKLKGLDMWMSYLLWFLVFLAKLVESYFFLTLSIRDPIRILSTTTMRCVGEVWYKEAVCRNQAKIVLGLMYLVDLLLFFLDTYMWYIICNCIFSIGRSFYLGISILTPWRNIFTRLPKRIYSKILATTEMEIKYKPKVLISQIWNAIVISMYREHLLAIDHVQKLLYHQVPSEIEGKRTLRAPTFFVSQDDNNFETEFFPRNSEAERRISFFAQSLATPIPEPLPVDNMPTFTVFTPHYSEKILLSLREIIREDDQFSRVTLLEYLKQLHPVEWDCFVKDTKILAEETAAYENGDDSEKMSEDGLKSKIDDLPFYCIGFKSAAPEYTLRTRIWASLRSQTLYRTVSGFMNYARAIKLLYRVENPELVQYFGGDPEGLEMALEKMARRKFRFLVSMQRLSKFKDDEMENAEFLLRAYPDLQIAYLDEEPAEEGEDARVYSSLIDGHCEMLENGRRRPKFRVQLSGNPILGDGKSDNQNHAVIFHRGEYIQLIDANQDNYLEECLKIRSVLAEFEELNVEHVNPYAPNLKSEELKDKKEPVAFLGAREYIFSENSGVLGDVAAGKEQTFGTLFARTLAQIGGKLHYGHPDFLNATFMLTRGGVSKAQKGLHLNEDIYAGMNAMMRGGKIKHCEYYQCGKGRDMGFGSILNFTTKIGAGMGEQMLSREYYYLSTQLPLDRFLSFYYGHPGFHINNLFIQLSLQVFILVLANLNSLAHESIMCSYNKDVPITDVLYPYGCYNLAPAVDWVRRYTLSIFIVFFISFIPLVVQELIERGVWKAFQRFVRHFLSLSPMFEVFVAQIYSSSVFTDLTVGGARYISTGRGFATSRIPFSILYSRFADSSIYMGARLMLILLFGSVSKWQAPLLWFWASLSSLMFSPFIFNPHQFAWEDFFIDYRDFIRWLSRGNTKWHRNSWIGYIRLSRSRVTGFKRKMIDDISEKSAGDASRAHRSNILFADFIPCLLYTAGLYVAFVFINAQTGVTEYPYEILGSRRPQPVNATLRVVICALAPVVIDMGILAVCVAMACCAGPMLGMCCKKTGAVIAGVAHGGAVIVHIVFFIVMWVLEGFNFARLLLGFATMIYIQRLLFKFLTLCFLTREFKNDKANTAFWTGKWYNTGMGWMAFTQPSREFVAKIIEMSEFAGDFVLAHIILFCQLPVLCIPLIDRWHSTMLFWLKPSRLIRPPIYSLKQARLRKRMVRKYCTLYFAILVLFVVIIVAPAVGSSQVAVDQFANIGGNGSIINGLFQPRNISNNDTGPNRPKSYTWSYFSTPEKGKTKGYSTNPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFA3</accession>
    <name>G3AFA3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Dicarboxylic amino acid permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_58021</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372304.1">
        <property type="nucleotide sequence ID" value="XM_007372242.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFA3"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:amino acid transmembrane transporter activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005310">
        <property type="term" value="F:dicarboxylic acid transmembrane transporter activity"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="entry name" value="General amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Amino acid/polyamine transporter I"/>
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    </dbReference>
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        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
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        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
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        <property type="entry name" value="APC_YAT"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0813">Transport</keyword>
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            <end position="71"/>
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    </feature>
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    </feature>
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    </feature>
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        </location>
    </feature>
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            <end position="211"/>
        </location>
    </feature>
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            <end position="267"/>
        </location>
    </feature>
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        <location>
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            <end position="305"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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            <end position="439"/>
        </location>
    </feature>
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            <begin position="460"/>
            <end position="478"/>
        </location>
    </feature>
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            <end position="511"/>
        </location>
    </feature>
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        <location>
            <begin position="49"/>
            <end position="522"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EGW34892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="573" mass="63791" checksum="4278BEDB51A4B8FB" modified="2011-11-16" version="1">MDIEKDGIQLEQTLSRHTVDYDLYVEKSNPSGIQLSDGHQLRQALDSRHVTMIAIGGALGTGLLIGTGSALKAAGPGAILVAYSTIGFCVFMVMSGLGEVATFVPLNGFANYCQRYVDDALGFACGYVYLFKYLVLPANQLVAGSLTVQYWVSRDKLNPGVWITIFLVIILAVNLLGVRFFGEIEFWLSTLKVLTCLGLILLLWIIALGGGPTHDRIGFRYWKDPGAFLKYADSSKGLVIEGSKGRFVAFVSVLVTAVFAYLGTELVGITFSETRNPRRSIPRAIKLTFYRILVFYILSIFWLGMCVSPKDPLLLSASGNTASASPFVIAIKNAKIGGLDHVINACILLFVLSAANSDMYVCSRTVYSLAVAGYAPKFFTKTNKLGVPYYGILLSFLFTLLAYMTVSEGSAEVFTYFVNVVSLTGLIAWSCILVIHIRFMQACKAQGIDRNKDLAYKSPLQPYGSYVALAICVLVIFIKNFTVFLGKPFTYKNFITGYIVLPVFLIMYFGYKFWYKTSIIKLHEVDLETYRDVVDAEFEKYEAEEAEKKAIREAEGKRFDKEWFYDKCIGWIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFB6</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
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        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
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        <lineage>
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            <taxon>Pichiomycetes</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58030"/>
    <dbReference type="eggNOG" id="ENOG502RQ31">
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    </dbReference>
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    <dbReference type="InParanoid" id="G3AFB6"/>
    <dbReference type="OrthoDB" id="4095746at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="238" mass="26917" checksum="183F8DA6720C1A3A" modified="2011-11-16" version="1">MGRHLKRQKIATISRDATSNQYNKVNDPNIIPPSFPPTKYEQSPATLKNFPSSIINGVTLEGGLYSLTFKSIACVDFVENNDLMRRSFRGSEIELSRITEKEVDLKGMIDDLKSDITKEKEEGNIIANDPITELSKDLQNRLEHKLELTDLNPILKQHKVLEEDKVLIAGIEQYHRVSGVNVPAIDINKTQVSSETKQTEEIVESKQPEQIEQQPQAVQPTEVVETSQQIVKEVENQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFI5</accession>
    <name>G3AFI5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">SPAPADRAFT_58098</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EGW34974.1"/>
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    </dbReference>
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    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58098"/>
    <dbReference type="HOGENOM" id="CLU_2151868_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFI5"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="112" mass="13145" checksum="CA628D5A75B539AE" modified="2011-11-16" version="1" fragment="single">MQRRSSSYMDDLLRQQSILDSPTSELSIHSEDMVLPLDDDNSLQTDTMTRVISLGRAQTIYTNDRYLQQRRRILSWPQKIFRWLYSFSLVIFIVLLLAFISVTPLDVIAQTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFR8</accession>
    <name>G3AFR8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein ERG3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ERG3</name>
        <name evidence="7" type="ORF">SPAPADRAFT_48108</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007372407.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="G3AFR8">
        <property type="interactions" value="198"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFR8"/>
    <dbReference type="GeneID" id="18871180"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48108"/>
    <dbReference type="eggNOG" id="KOG0872">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047036_3_0_1"/>
    <dbReference type="InParanoid" id="G3AFR8"/>
    <dbReference type="OMA" id="ICTPYAS"/>
    <dbReference type="OrthoDB" id="6354873at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000248">
        <property type="term" value="F:C-5 sterol desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="6">
        <location>
            <begin position="217"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="390" mass="45347" checksum="4F112DDE9E05CD89" modified="2011-11-16" version="1">MDLVLEVCDYYLFDSVYAKVFPKDGTVHQLLKNSPVAQVLNSIQLPFSLPEYDALSANSTLSTSFNISSLDAATSTIPNYLLYKLATFQNKSEVYGVTPNLFPAGPYFDSSFLGRSNTIRECLSLLVVASLFGWLLYFIVAYLSYVFVFDRKIFNHPRYLKNQMALEIHRASTAIPVMVMLTIPFWIAELKGLSFLYMDINEANGGWKEVAMQFPKFILFTDCGIYFIHRWLHWPSVYKRLHKPHHKWIVCTPFASHAFHPVDGFFQSLPYHIYPLLFPLQKVFYLGLFTFVNFWTVMIHDGNYMSNDPVVNGTACHTVHHLYFNYNYGQFTTLWDRIGGSYRRPDDSLFVKDSKAEDDKKLWKEQTVQMEEIRNELEGKVDDREYIETD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFW1</accession>
    <name>G3AFW1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sas10 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_48146</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372512.1">
        <property type="nucleotide sequence ID" value="XM_007372450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFW1"/>
    <dbReference type="FunCoup" id="G3AFW1">
        <property type="interactions" value="414"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFW1"/>
    <dbReference type="GeneID" id="18871186"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48146"/>
    <dbReference type="eggNOG" id="KOG3118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019106_1_0_1"/>
    <dbReference type="InParanoid" id="G3AFW1"/>
    <dbReference type="OMA" id="KSMKPVW"/>
    <dbReference type="OrthoDB" id="1924577at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007146">
        <property type="entry name" value="Sas10/Utp3/C1D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018972">
        <property type="entry name" value="Sas10_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237:SF8">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09368">
        <property type="entry name" value="Sas10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04000">
        <property type="entry name" value="Sas10_Utp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sas10 C-terminal" evidence="6">
        <location>
            <begin position="473"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="31"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="263"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="402"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="484"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="41"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="420"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="442"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="489"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="546" mass="62883" checksum="73C9555DACC0F6F9" modified="2011-11-16" version="1">MARQRIEEDVDLDEVDAFNANREKIFLDQAGEYANQQYSDESSEEEVMAVEEEDSDSDEEEQDDEEGMEDDDDDNELEEGEEEADEEKGWGGKKSYYGGDEVSDDEDMKQMTEEALRQQKKHLNELAMDDYIDEDMMQDWQKTAEAYEEKDTTRLHIATDTSIDKMDASEQQNLLKHSFPEFTPLLKELNQLKPKLESFLSREQNPLIEVKVVALSAYLGAITSYFAIFVDNLKTGDQFTSMKDEPIMESILSSREVWRQANELPEEVKETAHADNSFSSDDEEQFVDAKENYSDEDEEIEDEEEAEEEAEEEVEENGLAIDVTKQRNIKHASRHNADDFAESAEIEDVDLEDKKRRKKTLRFYTSKIDQAVAKQNKTFSGDMDIPYKERLFERQQRLVEEARKRGLAQSEDVGESGGESGDDFAQGEEDDYYDSIKQSKISKKESRKSAHEAAVKAAKEGKLAELQEEVGNDGKRAINYQILKNKGLTPHRKKEYRNSRVKKRKQYEKAQKKLKSVRQVYDSNKGPYEGEKTGIKKGLSRSVKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG16</accession>
    <name>G3AG16_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ammonium transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_48191</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372567.1">
        <property type="nucleotide sequence ID" value="XM_007372505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG16"/>
    <dbReference type="STRING" id="619300.G3AG16"/>
    <dbReference type="GeneID" id="18871191"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48191"/>
    <dbReference type="eggNOG" id="KOG0682">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_33_0_1"/>
    <dbReference type="InParanoid" id="G3AG16"/>
    <dbReference type="OMA" id="GCDFKFS"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029:SF10">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="349"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="378"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="408"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="449"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="9">
        <location>
            <begin position="22"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="9">
        <location>
            <begin position="344"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="593" mass="66882" checksum="A90CBABA13F97DA3" modified="2011-11-16" version="1">MTSSQTVDFKEIDVRTAINALYMIFCTALLPIVVIGIAFFYSGLTQRRSSLTMFAIPILLLPLIFIDWFIWGYSLSYSASANHFIGNLNFAVLRQLRNSITLVFTNTRGEILVLNHFLFNGFFKVICAALTMPGCIAERGRIIPMLVFLFFWSAMIYNPVTYWFWNSNGWLSIDLDNLPVLDFAGGNCIHIVGGFTCLAYSYILGPRNPKILYNYRNINTGHIIFGTFCILSGWVGFIAGCDFKFSSNSLYIMVNTLLAAFSGSIVWTSIDYFFSSIPLADTASITANPEQPAYPEPIIGGSSPESIELKRLHSPQSSHRIQPVISQTGILKSQRYHETKSNFVEKRKFSMISFSSGMITGLVVFTPGGGFVSSPSEFWKPIIFGVVGAIICNLSTRLKYFFGIDDALDIFAIHGVAGIVGSLLTGLFANTQYQSQGGWVKRHWIQFAYQLLGITVTSAYVFILTCVFLLIIDRIPGLHLRIDKNFNARQKQQKTTPELPIHELESQVSPHDVASNKDLFWEQVELQGTDTYEFNGEYMMDFIEFIKVIRPQDYEDNDQSFDNIDQPNYENLTGLGADYEMHPEGVHHLTKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG18</accession>
    <name>G3AG18_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58356</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372569.1">
        <property type="nucleotide sequence ID" value="XM_007372507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG18"/>
    <dbReference type="STRING" id="619300.G3AG18"/>
    <dbReference type="GeneID" id="18872414"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58356"/>
    <dbReference type="eggNOG" id="ENOG502R5KJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_204779_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG18"/>
    <dbReference type="OMA" id="AMYMARN"/>
    <dbReference type="OrthoDB" id="4011638at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="65" mass="7425" checksum="B7A548829E7F7C8D" modified="2011-11-16" version="1">MALVKVTPMQALAVGGVLYGLGAAWMFKNYFKQSSMRKIYTSADKEYEKVHVMRYPQYEGNAVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG83</accession>
    <name>G3AG83_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1748-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58427</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372634.1">
        <property type="nucleotide sequence ID" value="XM_007372572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG83"/>
    <dbReference type="GeneID" id="18872446"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58427"/>
    <dbReference type="eggNOG" id="ENOG502SA6M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151392_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG83"/>
    <dbReference type="OMA" id="MIQGTVV"/>
    <dbReference type="OrthoDB" id="16824at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013726">
        <property type="entry name" value="Mitofissin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28075">
        <property type="entry name" value="CHROMOSOME 16, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28075:SF1">
        <property type="entry name" value="DUF1748-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08520">
        <property type="entry name" value="Mitofissin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="12083" checksum="B757AC5B951A875B" modified="2011-11-16" version="1">MSIGKLTHYTFDLVLISIILAGIHRNTGLQFDVSHFSSVDVRRYFGKYLAFGEACYDRVVSLLKFSGYFRQSNLIYDFAQRASDRLKEETGRSLDEFKRENKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGF8</accession>
    <name>G3AGF8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Heat shock protein of HSP70 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_58517</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372709.1">
        <property type="nucleotide sequence ID" value="XM_007372647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGF8"/>
    <dbReference type="FunCoup" id="G3AGF8">
        <property type="interactions" value="1548"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGF8"/>
    <dbReference type="GeneID" id="18872482"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58517"/>
    <dbReference type="eggNOG" id="KOG0103">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_5_1_1"/>
    <dbReference type="InParanoid" id="G3AGF8"/>
    <dbReference type="OMA" id="WEQSPEI"/>
    <dbReference type="OrthoDB" id="434160at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000004">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000020">
        <property type="entry name" value="Heat shock SSE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF4">
        <property type="entry name" value="HSC70CB, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0346">Stress response</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW35297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="609" mass="67914" checksum="5EF4BBEB3A28ADAB" modified="2011-11-16" version="1" fragment="single">MSTPFGVDLGNTNTVIACARNRGIDIIVNEVSNRATPTLVGFGPKNRYLGESAKNQQTSNIKNTVDNLKRIVGLDYDHPDFAIEQQYFTVPLTKNEDGGINAKVRFLGKQTEFSATQLAGMYINKIKDIAFKETKANLTDICLSVPGWYTEKQRRAAADACKIAGLNPVRIVNEVTAAAVGYGVFKQNDLPEDEFKKVAFVDIGHSSYQVSIAAVKKGELKILGSANDKHFGGRDFDRAIAEHFATEFKGKYKIDVRENPKAYYRVLVAAEKLKKVLSANTQAPFNIESVMNDVDVSSSLTREELEEMVKPLLERINVPVELALQEAGIAVEDLDSIELIGGCTRVPSIKAKLAEIFGKPLSFTLNQDEAIARGNAYICACHSPTVRVRPFKFEDYNQYTVSYFWDKQEDEEDDHLEVFPRGGSFPSTKIITLFRSGDFEIEAKYTKPEELPAGTDVNVAKWKISGVVPSEGEASIATKVKLRNDPSGFYTVEAAYTVEEQLVKELIEPEVKEGEEADEDAEPQYREVKKLVKKNDCTIELESAALPPAKLQELIEQENAMVMEDKLVADTEDRKNALEEYIYDLRGKLDDQYSEFASPEEKEKLSGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGK1</accession>
    <name>G3AGK1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_48345</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372752.1">
        <property type="nucleotide sequence ID" value="XM_007372690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGK1"/>
    <dbReference type="FunCoup" id="G3AGK1">
        <property type="interactions" value="189"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGK1"/>
    <dbReference type="GeneID" id="18871207"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48345"/>
    <dbReference type="eggNOG" id="KOG0032">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010228_2_0_1"/>
    <dbReference type="InParanoid" id="G3AGK1"/>
    <dbReference type="OMA" id="MICYFIV"/>
    <dbReference type="OrthoDB" id="1405469at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110031">
        <property type="term" value="P:negative regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF190">
        <property type="entry name" value="MITOSIS INHIBITOR PROTEIN KINASE MIK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="659"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="686"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="711"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="166"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="34"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="740" mass="85608" checksum="97ED5A941FC3486B" modified="2011-11-16" version="1">MSIIPYNSNSDILFHDPNHGILVVHDNQENSISLVSTRSSRDKSRTRPPQPSSTFTNQQYGHYSGEKSTKCPNCGFTWSEYPEDTPRRNSRSSVTPFNFSLPDINLPRDFAQGFVRNDYFKLLGQLPYVSNNTNHENTNNHVNNHARSSLPEGIFNQGYFKRFFKRVEPFVLGSGAHAQVYKVDHVLNDIKLGTYAVKRISIGDKFEHLEQVLNEVLILYELSVKGANENNLIRYNHVWLELGDLEDSSSYFLPPSGVGEKVKNRIPYVFILQQYCDGGHLEDLISKHFRREENLTWKEKIDLERMKRRLRRSSSGNKQEETKKKKWLSNFEIWKFFHDVAKGVHYLHVHGILHRDLKPSNCLLDVEYIAREDSPEENQFLNLEEFENGVLDLPRVLVSDFGEGKFIDKKHNVVIEDQFNERRGNTGTLEFTAPELWLYSNDPILGEDNKTFFNDFTYESDIYSLGLILLYLCVGKLPFSDVIKGETDPQEIRNKIISWYDELTYELFQEWFTTNALELRDSFDDTMLDFQKLVYMMIKGEGSGNQSTSRAISKEVLEFLENMKRERFIKHYEEEEVVVSGEELMNRKSSAAGSLVRVSSGTIMDEPVLDEDEEEDEAFEEEHKSEHLNLLEGVRELDEFEGVNLNKTEPDAPTSSSSIHNVLAAIVLYGFELIVLEYISYNSAKLVNSILKVVVFFLVAIELYIENYTLLKTVLFVITSIAVSILLAYYLKFESSRDYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGL5</accession>
    <name>G3AGL5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_64490</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372766.1">
        <property type="nucleotide sequence ID" value="XM_007372704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGL5"/>
    <dbReference type="GeneID" id="18874977"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64490"/>
    <dbReference type="eggNOG" id="ENOG502T045">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_426986_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGL5"/>
    <dbReference type="OrthoDB" id="4026658at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="569"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="299"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="352"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="705" mass="80230" checksum="71E817817BDD7DA7" modified="2011-11-16" version="1">MSLTTTATKVHQPRSQPRMQPVLPAQDTPQTQLQNQNHTQTKLETTKPPSPQHIESTPATTTSTTTTTSSLPSSQQSSPITSGRIRQLVQPPDDYIKPYSHDKLYKTFTYTIILLPTLTSILFPTTTTTNTTTNEQIGNFIIDLLTVLLISWVVRYAVEWPFNWLKTLRKTKRALVQELLDSSEDQDMTKQILLIRKINTFEMIALVSCLLSSMFGSILLIWTRNYTIIDKVRKKMVFNNVNIALLQFWSIFRIFLTFTDILQNSSLNEGDLYIQQHHPRQSRKWYLELKDYFLPNPTNQILAENIQLYNKEFDQLKADLSNLQQIIKKFPSDSFTPFPLSINSGSVGSSPLSSASSSPIVGPASPVMQSKQPTMPKRVSTKQQLQQQLVPKKANRHFVATAVAKMQPPLKTIVEEDPGGAHEGNLSKKGLAIKISPPKSEPKKYINNSVRLDGKLKVLHEISLFDLLIHPGRVYRLIMDEIIYPYVTYQLQSKSYLLVKMVIWEVVNKYVLSGIFAWFLELYYNPFRYIKEVMVWICFKMPVRATITTLKTTVFVPQMILRWVLLKPLYLGMVIVFGTTSVFFSSIRSSKKKKERNVEPVKVIRRRRVPVNNSPVAVTPPFTGTEFPGMKQFHLNSPEERNFNGVSKIASILNRPQVQKRSGSPSIKPALCPPLVATMTSNAPMPATSSSFRRFNVSPVSLYDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGR0</accession>
    <name>G3AGR0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glutathione S-transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_58610</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372805.1">
        <property type="nucleotide sequence ID" value="XM_007372743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGR0"/>
    <dbReference type="STRING" id="619300.G3AGR0"/>
    <dbReference type="GeneID" id="18872531"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58610"/>
    <dbReference type="eggNOG" id="KOG0867">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_14_2_1"/>
    <dbReference type="InParanoid" id="G3AGR0"/>
    <dbReference type="OMA" id="GHSGAEY"/>
    <dbReference type="OrthoDB" id="422574at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03048">
        <property type="entry name" value="GST_N_Ure2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF8">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE GSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01151">
        <property type="entry name" value="Main.2:_Nu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="3">
        <location>
            <begin position="2"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="4">
        <location>
            <begin position="92"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW35393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="213" mass="24174" checksum="3BBEF12744771810" modified="2011-11-16" version="1">MSHALKLYSFPTPNGVKVSIFLELLGLKYDAQAVDIRTNEQKQDWFVKLNPNGRIPTLVDSSTGVTISQTGAILQYLADTYDKEHKWSYPQGTPEYYKQLEYLIFQVAENGPIQGQANHFVVFAQEKIPYAINRYLTDVKRIYGVYEDILGRNDKGLYLVGDHYSIADIALFGWANSLSKLDIDIHTWPKLGKWFDALSEIPGVQKGLKVPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH87</accession>
    <name>G3AH87_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ferric-chelate reductase (NADPH)</fullName>
            <ecNumber evidence="3">1.16.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_146859</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2 a Fe(II)-siderophore + NADP(+) + H(+) = 2 a Fe(III)-siderophore + NADPH</text>
            <dbReference type="Rhea" id="RHEA:28795"/>
            <dbReference type="Rhea" id="RHEA-COMP:11342"/>
            <dbReference type="Rhea" id="RHEA-COMP:11344"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.9" evidence="3"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372929.1">
        <property type="nucleotide sequence ID" value="XM_007372867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH87"/>
    <dbReference type="FunCoup" id="G3AH87">
        <property type="interactions" value="394"/>
    </dbReference>
    <dbReference type="GeneID" id="18870729"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_146859"/>
    <dbReference type="eggNOG" id="KOG0039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010365_4_0_1"/>
    <dbReference type="InParanoid" id="G3AH87"/>
    <dbReference type="OMA" id="YNEDAHM"/>
    <dbReference type="OrthoDB" id="167398at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF9">
        <property type="entry name" value="FERRIC REDUCTASE TRANSMEMBRANE COMPONENT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="7 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442503" description="ferric-chelate reductase (NADPH)" evidence="17">
        <location>
            <begin position="20"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="373"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="18">
        <location>
            <begin position="407"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW35517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="740" mass="84342" checksum="3ED534AD2FA724AB" modified="2011-11-16" version="1">MNLLYSFYLLFFAVSGVVAIPGIEWYKPTLGLYACLYEIDYSGNFTFCPATDSTCLCSDPNSRATMAGCLVYHDRNTTIMTDYIVKYCGMYFETNLTSDWFEGAYANFTARAKYFKDIDMPADSNIVDVPLKFEPKDMDHYSTIAMNFLGNYDDSVYYGVSVYGFWLIVLLIGAASHWTKMLFPGLTKKMTGPVSNFWRKYISMPAFIGRRKAQSIPGFKIFDSLIPTRFEMLVISLFYVYMIIIHAINMKGVKGDPVFGSKYMAELRYVADRTGIVGTAMMPLVFLFAGRNNFLQWICGFNYSTFLCYHRHLARVMFMLIVIHSVNFTILEEEYYAEDIKEPWLYWGVVATIVGGVMLIQSILYLRRINYEIFLLVHIVLATFWVVGTWVHVVDFGYGCVFIPSIAVWGFDRAVRIGRLLYFGFPEVTIALVGDETIKLSIPKPKNWHPIPGGHAFIYFLKPTYFWQSHPFTFTSSAKEDHTIVMYIKLKGGITHSLYKLLNKSPGKTVTMRVGVEGPYGESTPAKYVDKAVFIAGGNGIPGIYSEVHDIAVRSTKERNQVLKLIWVIRDYGSIAWFHDELAALQNTNIDTTVYVTKPSANLGKIEQLDKSDSSKEGSIKSSELTKSYEANLTNISDINEKITSSKEDSINEVSKSKEYITDDIPGELKEKLSHIHFEEGRPNIERLIQDEIKESPGSTFFVACGHPIMVDEVRYQCCKNIDNPEKKRVDFFEQLQIWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHB3</accession>
    <name>G3AHB3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_54277</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373661.1">
        <property type="nucleotide sequence ID" value="XM_007373599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHB3"/>
    <dbReference type="FunCoup" id="G3AHB3">
        <property type="interactions" value="341"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHB3"/>
    <dbReference type="GeneID" id="18871847"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54277"/>
    <dbReference type="eggNOG" id="KOG0061">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_57_1_1"/>
    <dbReference type="InParanoid" id="G3AHB3"/>
    <dbReference type="OMA" id="FNCQLDA"/>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000702">
        <property type="entry name" value="ABC transporter (Adp1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF2">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="288"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="744"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="776"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="820"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="856"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="888"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="972"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="346"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="998" mass="111790" checksum="72E210169F7198A3" modified="2011-11-16" version="1">MQDTFEIKTEGRKGGFGGGKDEDKECPPCFNCNLPNFECTQFSQCNAFTGMCECQDGFGGMDCSEPLCGALGDGNNNRPVRKEKDPHCKCKSGWTGINCNMCTHDDVCDSFMPEGLKGTCYKQGIIVNKLHQMCDVTNPKIISILNGKKPQATFACNRTSENCNFQFWIDELESFYCDLNKCKLDYDLGANTTHYNCEEVACKCLPDRMLCGQDGSIDISDFLTETIRGPGDFACDISSKSCKFSEPSMNDLIQSVFGDPYITLKCQSGECVHKSQIPGYEVPDHSKLTLGSIFSIIGVFVVCVLIVVTAVRNISESALFKKDPYRGEPFEADASVLNQNFEPTTLSFENISYEVTGGIRVLNEIFGVVKPRECLAIMGGSGAGKTTLLDILAGKNKGGKIYGNIYVNGKVIDPTHYKAIVGFVDQEDHLIPTLTVYETVLNSALLRLPRDMSFTQKQARVIEVLNELRILNIKDRVIGSDFKRGISGGEKRRVSIACELVTSPSILFLDEPTSGLDSYNARNVVDCLVKLSRDYDRTIVFTIHQPRSNIVSLFDKLLLLSEGDLIYSGDMIKANDFFTRNGYNCPLGYNLADYLIDITVDHKKIVKVTNADSDIVAATSTSTDIQDVDIHDAFLKNRASTQEDTTLEWEHFATHRDEYNNEIIGRRTSKTGEEETYIRLRNKLPTLFVESSLALELKQEIEEGKQHHTSLDLTKHDIKKATFFNQAAILSSRTFKNLYRNPRLLLAHYVLSLLVGLFCGYLYYDLSYDISGFQNRLGLFFFLLAFFGFSSLTGLHSFATERIIFIRERANNYYHPLSYYLSKIFCDLVPLRVLPPVILISVAYPLVGLTMEHNAFLKAICVLVVFNIAVSTEMLIVGILIKEPGTSTMIGVLLLLLSLLFAGLFINSEDLTISIKWLEWVSVFHYAYEALSINEVKDLILREKKYGLSIEVPGAVILSTFGFDVGAFWKDVLYLAGLSLLFLVFGYVFLHKFAIERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHB4</accession>
    <name>G3AHB4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 9">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="1 9">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_148465</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="1 9"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373662.1">
        <property type="nucleotide sequence ID" value="XM_007373600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHB4"/>
    <dbReference type="STRING" id="619300.G3AHB4"/>
    <dbReference type="GeneID" id="18870784"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_148465"/>
    <dbReference type="eggNOG" id="KOG0660">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="InParanoid" id="G3AHB4"/>
    <dbReference type="OMA" id="FINNHPY"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071507">
        <property type="term" value="P:pheromone response MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046827">
        <property type="term" value="P:positive regulation of protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010526">
        <property type="term" value="P:transposable element silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000098">
        <property type="entry name" value="Mitogen-activated protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="40"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="395" mass="46002" checksum="E4B792D077F6C841" modified="2011-11-16" version="1">MIVNKFNRYNQLKNSNQQSRGSINQSQPLRKVVFNVSESLKVVKILGEGAYGIVALAIHTPTGTKVAIKRIEPFERPLFCLRTLREIKLLSKFDNHENIIKLYDVQKPYNYQMFHEVYLIQEYMPSDLHNIIRTHTLTDQHVQYFVYQILKGLKLIHSAKVIHRDLKPSNLLVNEECDLKICDFGLARLDNQLYPNQELPNNISSLTEYVATRWYRAPEIMLNAANYSRAIDIWSVGCILAEMFTYKPLFPGSDYVHQLRLIFEIIGSPSEEDMHIVKSHRAKNFLKSLPHREKLDFTEYINTHPYRIAKHGINNVNPLGVDLLEKMLVFDPNKRITVDEALAHPYLASYHDPLDEPITSPIPLEEFAFDINKDDLDKENLKRQIYNQIITAKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHS0</accession>
    <name>G3AHS0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UBR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_49293</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373819.1">
        <property type="nucleotide sequence ID" value="XM_007373757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHS0"/>
    <dbReference type="STRING" id="619300.G3AHS0"/>
    <dbReference type="GeneID" id="18871323"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49293"/>
    <dbReference type="eggNOG" id="KOG2752">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025221_1_0_1"/>
    <dbReference type="InParanoid" id="G3AHS0"/>
    <dbReference type="OMA" id="GAMVYNH"/>
    <dbReference type="OrthoDB" id="2411602at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19677">
        <property type="entry name" value="UBR-box_UBR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040204">
        <property type="entry name" value="UBR7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047506">
        <property type="entry name" value="UBR7-like_UBR-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13513">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13513:SF9">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="5">
        <location>
            <begin position="40"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="4">
        <location>
            <begin position="40"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="412" mass="47376" checksum="4CCACC542F8990F7" modified="2011-11-16" version="1">MTDPDTTTTNASESVTAVDYITNQVRLEREARELMPYDPNECTYELGELRQPVFACLTCSRQNDDTPIGVCYSCSIQCHASHDIVELFSKRGFVCDCGTTRMSKTHNGACKLRRHGHKLERRLSISSNSSAKELELNAEDIPSSSNSYNQNYRGTFCDCQEQYNPAEETGDMLQCYFGFECGEDWYHDRCIMGIDKSAVSKTDNGEEPKLKYFPNLVDFDVFVCWKCISKFKQVFDELDRQTTIVHTKLPHFKQVPSVEEWHKQKETFEQTSAEPAAKKAKVDTTDVATEAPLYSYFLSNDFRDQIKKLYPKLPSESKLHQFLSNNNYLFEDDPVYEPPQDDDSETGSILDMGTDALHSLPRDKAIEGLQAYEQMRSKLRDFFKPFAEQGKIVTEDEVRQFFGSMTEERKHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHX1</accession>
    <name>G3AHX1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial inner membrane i-AAA protease supercomplex subunit MGR3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_133779</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373869.1">
        <property type="nucleotide sequence ID" value="XM_007373807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHX1"/>
    <dbReference type="FunCoup" id="G3AHX1">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHX1"/>
    <dbReference type="GeneID" id="18869767"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133779"/>
    <dbReference type="eggNOG" id="ENOG502QU02">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039436_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHX1"/>
    <dbReference type="OMA" id="CKMTTVE"/>
    <dbReference type="OrthoDB" id="10050400at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031942">
        <property type="term" value="C:i-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24145">
        <property type="entry name" value="Mgr3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040201">
        <property type="entry name" value="Mrg3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28142">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE I-AAA PROTEASE SUPERCOMPLEX SUBUNIT MGR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28142:SF1">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE I-AAA PROTEASE SUPERCOMPLEX SUBUNIT MGR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="518" mass="59431" checksum="79921902E30A706B" modified="2011-11-16" version="1">MTTRILISQASRRLVRNKYVSNPLLVLARFNSSQGQYNYNYNQSYNFQPPQPRTSIFKKFLYTTLGFIGLTGAAYYIWWPKHTFPSSVAKILRKGLWAESDKGENDYQLALKYYLQAQEHCQEINMDPLSDEYTGIQLKIAEMFERLNMKADASFVYNEIATLYLTVLTASPDSKHGKRLKSREHRRHLIQKDLRIAIKLVEMNRENPQLCKAILVTHLIIAQDEVNKVLTSPKPLSLVNRADSKDDVNTYTATLDNDTIVINGNHGEEFRVKKTPEVWEPFTDEFFNAMDLLSAICISSGDLAMASKVKISMSEAMLLADVEPSKMLLSQCNLGSLLYMQAEELEAQEISLRRKFSDLTGIEYERLKTESLLQDQDDKESIQETLKTKVSAEDQELYDKVVSSKNVCIQLAIKSFESVLEFAKTVPQEIIKQHNEINETVALATYGLGVVNLHLGAYDKAERLLRESRVRSRKCNYSYLIPEIERELGKLFDEKKSMKSGIPRKDDLRDIEMDILLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHX7</accession>
    <name>G3AHX7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF202 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_59708</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373875.1">
        <property type="nucleotide sequence ID" value="XM_007373813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHX7"/>
    <dbReference type="GeneID" id="18873040"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59708"/>
    <dbReference type="eggNOG" id="ENOG502S455">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053359_3_0_1"/>
    <dbReference type="InParanoid" id="G3AHX7"/>
    <dbReference type="OMA" id="GMTRYFS"/>
    <dbReference type="OrthoDB" id="199599at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052053">
        <property type="entry name" value="IM_YidH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187:SF2">
        <property type="entry name" value="DUF202 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187">
        <property type="entry name" value="FGR18P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="DUF202" evidence="8">
        <location>
            <begin position="66"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="182" mass="20170" checksum="341FF8B8A54E1A65" modified="2011-11-16" version="1">MSAAQSPSYGSIDDHNRPTPRPSPSLRAQEAPPTLDNIETHKNDNILDRLGISTSYTLENKGSVARDHMANERTFLAWLRTSLAFITIGIGVTQLLRIDRPGSTPNSKFEVNQAISLKLGKTIGSIFIIIGILSLLFGCQRYFEVQNMLTKNHYPAARLGILALIVLVLTIILFTFFMVIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHZ9</accession>
    <name>G3AHZ9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Isoleucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_54466</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="2"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373897.1">
        <property type="nucleotide sequence ID" value="XM_007373835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHZ9"/>
    <dbReference type="FunCoup" id="G3AHZ9">
        <property type="interactions" value="752"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHZ9"/>
    <dbReference type="GeneID" id="18871864"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54466"/>
    <dbReference type="eggNOG" id="KOG0433">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_7_2_1"/>
    <dbReference type="InParanoid" id="G3AHZ9"/>
    <dbReference type="OMA" id="HCWRCKT"/>
    <dbReference type="OrthoDB" id="10264412at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07960">
        <property type="entry name" value="Anticodon_Ia_Ile_BEm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02002">
        <property type="entry name" value="Ile_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033708">
        <property type="entry name" value="Anticodon_Ile_BEm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023585">
        <property type="entry name" value="Ile-tRNA-ligase_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050081">
        <property type="entry name" value="Ile-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42765:SF1">
        <property type="entry name" value="ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42765">
        <property type="entry name" value="SOLEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="10">
        <location>
            <begin position="50"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="11">
        <location>
            <begin position="719"/>
            <end position="879"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW34313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="968" mass="111006" checksum="041D7BFAA950DCA5" modified="2011-11-16" version="1">MLRCRKQPFSTTVLRLNKISYSHTLLLPQTKFSPKLPQGDLRESLINKTSQDLYAWQSEHNSDKKPFVLHDGPPYANGDLHLGHALNKICKDIINRFQLIHNNALIKYRPGWDCHGLPIEMKVNPHQTIRDPLEIRQACRDLATTMIDRQRQQFLQYAIMTDFTTPYITMDHSYEINQLKVFKKLMERGLLSRQLKPVWWGCDTGTALAEAELEYNDNHTSVAIHVKFPVVSENLYEYIKNNYQKTIEKIQLLIWTSTPWTIPANRAVCVNEHMTYTLITNNSEYLVVAKPLVEHVLSLNSDYKQTNVEIPGSVLVGNTYTNPGSKKDQQYPVLHGDHVVDNAGTGLVHNAPAHGREDYLVAKKYDLSIDSVIDEAGKLVEATLAPGFQSLAGFKVTEPKTNVICANILAENNMLFHINKKFKHSYPYDWRSKTPVVQRATPQWFVNVDKIKPLALEAISKVKFVPETGKHRLASFIENRSEWCISRQRVWGVPLPLIYNKHTNEPVLDLAVVDYLIGRLDENGTDQWFVDEVDISRWLPSESESAELGIPFINGADYYKGKDTMDVWFDSGTSWSTLENQDEPADIYLEGSDQHRGWFQSSLLNKVITSNSAPFKSVITHGFITDGKGQKMSKSLGNVFSPQEAIEGCKKPRTPYLGPDGLRTWVASSNYKSDVNFSPEVLTRVSEISKKYRVTFKYLLGNLYDRGEPVAYEELADLDKFTLHSLYKLQQECVDFYQEHNFSRVMNSLNLHVNSMLSAIYFDISKDCLYTDKKDSLKRRSIQTVLDKILVTYIGLLAPIQPLLTQEVWTSYREIANVEADSPFKLEWNTSYQLPQQYENEQLAQEFSQFFSLRDETFRLMEVVKKQTELKNKLQYEVVLTVKPGSPIHTFLQRNVAHLDDFFLVSNVILEQGDVSAAEKDDATSIWNDQVAVQLRPSPDHKCPRCWKFTAPKEDELCHKCHEVVDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI07</accession>
    <name>G3AI07_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytoplasmic tRNA 2-thiolation protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">NCS2</name>
        <name evidence="3" type="synonym">CTU2</name>
        <name evidence="4" type="ORF">SPAPADRAFT_59736</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CTU2/NCS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373905.1">
        <property type="nucleotide sequence ID" value="XM_007373843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI07"/>
    <dbReference type="FunCoup" id="G3AI07">
        <property type="interactions" value="191"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI07"/>
    <dbReference type="GeneID" id="18873056"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59736"/>
    <dbReference type="eggNOG" id="KOG2594">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024534_1_0_1"/>
    <dbReference type="InParanoid" id="G3AI07"/>
    <dbReference type="OMA" id="EGDSTWA"/>
    <dbReference type="OrthoDB" id="25129at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03054">
        <property type="entry name" value="CTU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019407">
        <property type="entry name" value="CTU2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20882">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20882:SF14">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10288">
        <property type="entry name" value="CTU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="381" mass="42911" checksum="3570B91F89509D89" modified="2011-11-16" version="1">MQDDKYKVKYGEVAEKLGKQKVLLALSGGESSLVLLDIMSSLLQEQFEAHKGRQGFELVVVNLDEYKLKSLNSQCQDVIPKLLERYTQVKLSFKVLSLDSYIDQDTLKYISLNKDFTGFVQELHRTKEYTLNELLESCQDKSSAEDLLSIVYESLILKTAEVEGCETIVYGHSMTRIANEIIALTIKGRGSIIHKAIADHTTTYNNKEFKILFPLRDVLYVELLAYAQLAELTPYILISTIQKSKITKNLTIRDLTTNYFKNLDATGYASTASTVVKTGEKNITVNDAAPVTTDEEMKYLELFKASLETGGVSEGVAKEPVSICYACIVTLSGVKKDNFIWPVIDDKPQHDTPLQFVYTNDEEEKKKVLDEFILTDDEEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI58</accession>
    <name>G3AI58_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_54517</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373956.1">
        <property type="nucleotide sequence ID" value="XM_007373894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI58"/>
    <dbReference type="STRING" id="619300.G3AI58"/>
    <dbReference type="GeneID" id="18871872"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54517"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028814_5_0_1"/>
    <dbReference type="InParanoid" id="G3AI58"/>
    <dbReference type="OMA" id="ATHERKK"/>
    <dbReference type="OrthoDB" id="427030at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001907">
        <property type="entry name" value="AZF1 (YOR113W)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002157">
        <property type="entry name" value="Transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001289">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF142">
        <property type="entry name" value="ZINC FINGER PROTEIN 384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="182"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="276"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="318"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW34372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="328" mass="36517" checksum="94554FF1CFBCC901" modified="2011-11-16" version="1">MGSPPKPVSSSPRDRNLVPAQQFAKSEDGRPLLGATKIDQLMLVIQARDKGVTKTIQQAPDGSILAPAPGAVFKQEADVLPAPVSLVGGVDKPHKKIEDEVDEEGLVGGEDEEHHHKHRRSKHKNQQCPYCFKYFTQSTHLEVHIRSHIGYKPFECTYCHKKFTQGGNLRTHLRLHTGEKPFTCDVCHRSFSRKGNLAAHKLTHENLKPFECKLDGCDKSFTQLGNLKSHQNRFHLNTLNMLTHRLAELSGDGLNNLPQEERELLDYFKDLYKNSNKGIRGRGKQHKDEPKTPGGNGGMMMMTSPNFPGGPSPEYGNQLGGTISGYQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIH5</accession>
    <name>G3AIH5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Acid phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_59874</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374029.1">
        <property type="nucleotide sequence ID" value="XM_007373967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIH5"/>
    <dbReference type="FunCoup" id="G3AIH5">
        <property type="interactions" value="476"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIH5"/>
    <dbReference type="GeneID" id="18873113"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59874"/>
    <dbReference type="eggNOG" id="KOG1382">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020880_3_1_1"/>
    <dbReference type="InParanoid" id="G3AIH5"/>
    <dbReference type="OMA" id="RGYSPFC"/>
    <dbReference type="OrthoDB" id="6509975at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016274">
        <property type="entry name" value="Histidine_acid_Pase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963:SF18">
        <property type="entry name" value="ACID PHOSPHATASE PHO11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963">
        <property type="entry name" value="MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000894">
        <property type="entry name" value="Acid_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="62"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="260"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="403"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000894-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000894-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="463" mass="52190" checksum="C994EB046719BB66" modified="2011-11-16" version="1">MVAISRILSPGLLLVSQDQFRGVAAPELAAESQYNIVKYLAAAGPYIQNPGFGISTDTPDQCTIEQVQLYMRHGERFPGLDAGIHHKQIVDKLQSYPDTLMGDLAFLNYYKYYATEDLYEYETTPQNSPGPYTGYETAIRAGAAFRAKYGHLYDDSKKLPIFIAASYRVYETALNFATSFLGEKYSQDKLNKVIISENATMGLNSLVPRWGCPAYKPNGNKDLVSKFPTQYLEKIVTRFQKSNPGLNITTDDVSQLFQICAYEMNSRGYSPFCDLFTEDEYVTYGYNQDVFFYYQSGPGGVNSKLAGSVQLNATLNLLKEDNPENKIWLTFTHDTDIELFSSALGLFDPVTPLPVDQVRFYDSYHHVDVIPMGGRFITEKLKCGDTFYVRFVVNDAVIPVPGCSDGPGFSCKLDKYEAYVLDRIGKYDIARDCKVPDNVPNFLSFYWDYKTVNYNASVPRQTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIN5</accession>
    <name>G3AIN5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquinone biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_59114</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Membrane-associated protein that warps the membrane surface to access and bind aromatic isoprenes with high specificity, including ubiquinone (CoQ) isoprene intermediates and presents them directly to Coq7, therefore facilitating the Coq7-mediated hydroxylase step. Participates in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the COQ9 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373333.1">
        <property type="nucleotide sequence ID" value="XM_007373271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIN5"/>
    <dbReference type="FunCoup" id="G3AIN5">
        <property type="interactions" value="258"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIN5"/>
    <dbReference type="GeneID" id="18872779"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59114"/>
    <dbReference type="eggNOG" id="KOG2969">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057411_1_1_1"/>
    <dbReference type="InParanoid" id="G3AIN5"/>
    <dbReference type="OMA" id="SELFMAQ"/>
    <dbReference type="OrthoDB" id="619536at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.10">
        <property type="entry name" value="Tetracycline Repressor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013718">
        <property type="entry name" value="COQ9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012762">
        <property type="entry name" value="Ubiq_biosynth_COQ9"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02396">
        <property type="entry name" value="diverge_rpsU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21427">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21427:SF19">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08511">
        <property type="entry name" value="COQ9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 8" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="COQ9 C-terminal" evidence="9">
        <location>
            <begin position="154"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="223" mass="25297" checksum="1A5C790B7A0C5C1A" modified="2011-11-16" version="1" fragment="single">MLKTLSKTAKPRCGGSLLRLYHSSEHIGSNVIVDNNTIESKILSKALEHIPRYGFKTGSITKAIRDLEYPDSIQSVITSNPKGNSAEFQLTLHWLKSKRQELNNYAAEDPEFNSITNEYERVAKLIKTRLMMNSPVIGHLHEGLSQLVVPYNLPQSMEELHNLSDDIAFLAGDMSHDFAWYSKRAGFSSIYVKSELYMLQDSSANFKKTNKFVDEKVRGIKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIY6</accession>
    <name>G3AIY6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inorganic phosphate transport PHO88</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59151</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373381.1">
        <property type="nucleotide sequence ID" value="XM_007373319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIY6"/>
    <dbReference type="FunCoup" id="G3AIY6">
        <property type="interactions" value="253"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIY6"/>
    <dbReference type="GeneID" id="18872795"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59151"/>
    <dbReference type="eggNOG" id="KOG4554">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099163_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIY6"/>
    <dbReference type="OMA" id="NMDYDKG"/>
    <dbReference type="OrthoDB" id="18139at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012098">
        <property type="entry name" value="SND3_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28112">
        <property type="entry name" value="SRP-INDEPENDENT TARGETING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28112:SF1">
        <property type="entry name" value="SRP-INDEPENDENT TARGETING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10032">
        <property type="entry name" value="Pho88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008756">
        <property type="entry name" value="P_tr_PHO88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="191" mass="20895" checksum="81EF64954A9537B2" modified="2011-11-16" version="1">MNPAVSNIVIMLVMMQLAKRLDFEDPDVLFYTRAAYVSCQLVAFAIYFIVRAKINAKNDLTTLKYVEPANPLSGDAEPKAVVTTVKEYDLQQVNTQVKGMFTGLAMMAFMHLYMKYTNPLFMQLISTVKSALESNIVKIHLFGAPASGDLKRPFKAAPGFMDALTGAGAVKTDKQSIEEAEVSGAGGIKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ02</accession>
    <name>G3AJ02_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Actin-related protein 2/3 complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_54639</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Cytoskeleton</location>
            <location evidence="6">Actin patch</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ARPC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374099.1">
        <property type="nucleotide sequence ID" value="XM_007374037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ02"/>
    <dbReference type="FunCoup" id="G3AJ02">
        <property type="interactions" value="740"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ02"/>
    <dbReference type="GeneID" id="18871877"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54639"/>
    <dbReference type="eggNOG" id="KOG1876">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084855_1_0_1"/>
    <dbReference type="InParanoid" id="G3AJ02"/>
    <dbReference type="OMA" id="EAYLGEF"/>
    <dbReference type="OrthoDB" id="2264294at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1460.20:FF:000001">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034666">
        <property type="entry name" value="ARPC2/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008384">
        <property type="entry name" value="ARPC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22629:SF0">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22629">
        <property type="entry name" value="ARP2/3 COMPLEX 20 KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05856">
        <property type="entry name" value="ARPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039100">
        <property type="entry name" value="ARPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69645">
        <property type="entry name" value="Arp2/3 complex subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR039100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="171" mass="19908" checksum="07760DD1D8D2D9EA" modified="2011-11-16" version="1">MSQSLKPYLTAVRASLTAAICLQDFSSQLVERHNRPEIEVDNSHDPELILNPMVIARNENEKILIEPSINSVRISIKIKQADEIEQILVHQFTRFLTSRAENFFILRRVPIKGYDISFLITNFHTEQMLKDKLVDFIIEFMEDVDKEISEMKLFLNARARVIAESFLIPFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ43</accession>
    <name>G3AJ43_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="8">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_59977</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="8"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374139.1">
        <property type="nucleotide sequence ID" value="XM_007374077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ43"/>
    <dbReference type="FunCoup" id="G3AJ43">
        <property type="interactions" value="359"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ43"/>
    <dbReference type="GeneID" id="18873163"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59977"/>
    <dbReference type="eggNOG" id="KOG0787">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023861_4_1_1"/>
    <dbReference type="InParanoid" id="G3AJ43"/>
    <dbReference type="OMA" id="WSYPPSA"/>
    <dbReference type="OrthoDB" id="3264224at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF20">
        <property type="entry name" value="[3-METHYL-2-OXOBUTANOATE DEHYDROGENASE [LIPOAMIDE]] KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="9">
        <location>
            <begin position="293"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="437" mass="50586" checksum="68E1C6EC664882B4" modified="2011-11-16" version="1">MITRARCVTWRIPAINPFRTYVQSQSHPISVCEDEVGQQERFLEEYKIRSLLERPIVQYSQKPLPKFSLKQLYRQSEKLTSNTILKNAYDTVEHLLIYNARRLKEFRKLPYLVVLNPSISESYDTYLHTMSSLLRASLYPPHTLKENEEFAEQVLTEFIDVHADTLPSLSKGFSEVSNLLTHQQIKEFLDDHLRERISMRLIAHQHLELTKSINNPDRYVPGGKYNGVIKMLNIPDVIKKNADIVNDITMLKYDQAVKVQIDSNQYQGNYWSQSEPTLEINQELTFPYIEYHLDYILMELFKNSFRAHIENKVDQPVQVTISISRNPLFLELRIRDRGKGIPTKAYKHIFDYSYTTYESGEGEANKTLNVPPGLGGNTVAGMGYGLPLSKQYIEIFNDTLVPNDDDWEVEIPPTKGSLTVQTYNGWGTDVYLKTVGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ59</accession>
    <name>G3AJ59_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein SCD5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_133792</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373400.1">
        <property type="nucleotide sequence ID" value="XM_007373338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ59"/>
    <dbReference type="STRING" id="619300.G3AJ59"/>
    <dbReference type="GeneID" id="18869768"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133792"/>
    <dbReference type="eggNOG" id="ENOG502QQ7A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_485707_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ59"/>
    <dbReference type="OMA" id="QINHFQN"/>
    <dbReference type="OrthoDB" id="2553626at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="453"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="512" mass="56779" checksum="62629DD7CCD8D563" modified="2011-11-16" version="1">MSQTLSVNEPIISERDDNYIPLSLTAQDLTLQESKTYMRWYSDILARTSSRTITMNDVYNFLNNFKIPQDIKEKVNRIFSKILYSINIGEFFALLRVISHTLQGKEPSRALIAVAAPVPAPPSILSKKRQNDDDDDDANDSILNDEGSEINSNGSVQDVNKPLDIDSFTQFLLTGERPDEQPKKRKSKKLKSVKFSDQVDIHDDSFVNSPAESPQPQDHLDYSLPMDQLLNRMKNQPRQQQQQQRLKEEEEEREILKDMESQINHFQNLHSVDTVSIGGVPSSIHLQNSSENLLRPNMTGPAQMARMFSPSPDPELLQPNMTGPTQMAQYLNRTQNNVSPLMPNVTGPADMARIFAPGSSTDSEIEAPKISLQSFTSQMTGNTMENTLQNSQIGQQQESNNLLSRPLPPVPIRHRSASSPSPGTINGGPPPVPPRSPLSRVPPPPPPSRRRNASMSASVSASVVTPPPLPPKVPQSGVMYQASSNDSTSNILDDLKALQAEVDKIRDMTGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ92</accession>
    <name>G3AJ92_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">DNA dependent ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_133417</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373433.1">
        <property type="nucleotide sequence ID" value="XM_007373371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ92"/>
    <dbReference type="FunCoup" id="G3AJ92">
        <property type="interactions" value="672"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ92"/>
    <dbReference type="GeneID" id="18869745"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133417"/>
    <dbReference type="eggNOG" id="KOG0387">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_7_0_1"/>
    <dbReference type="InParanoid" id="G3AJ92"/>
    <dbReference type="OMA" id="PTWTGQF"/>
    <dbReference type="OrthoDB" id="413460at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061635">
        <property type="term" value="P:regulation of protein complex stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18000">
        <property type="entry name" value="DEXHc_ERCC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000039">
        <property type="entry name" value="DNA repair protein Rhp26/Rad26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.850">
        <property type="entry name" value="SWI2/SNF2 ATPases, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058951">
        <property type="entry name" value="WHD_Rad26_CSB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25875">
        <property type="entry name" value="WHD_Rad26_CSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="283"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="619"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="166"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="175"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="195"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW33849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1042" mass="119919" checksum="36E49D16F551F159" modified="2011-11-16" version="1">MNEPNESHESVDFQLVDQAELETSITRKANAALLQKDAELDQKRLDKAIKDLNTTIKRIDILSKKLDNPRTKISERKSLRDEIAWFQENELEPRQRDVEEIKARLDESNSKLNAGDEDDANEGRLPDETEKEFLIRTGKITAFGNENEFQQDDDDSDAAQVQSHVFLRQPGFEKEVEDDDYEYEPETEENKSDHDDEEEEDQDITEDFKNIDDGDESIYKSRLLDWCRRRSGLRQEDPNPNLDEWLKPHPTISDAILNSKFKLPGDIYPSLFDYQKTCVQWLWELYSQKTGGIIGDEMGLGKTIQIISFIAGLHYSGLLDKPVLVVVPATVLNQWVNEFHKWWPPLRCIILHSIGSGMGEKVSEEKLESFLETHDPHASTSSLRGIKSQINAQEIIDRVMEKGHVLITTYVGLRIYSKHILPREWGYVILDEGHKIRNPDSDISLTCKQIKTYNRIILSGTPIQNNLIELWSLFDFIFPGRLGTLPVFQQQFSIPINMGGYANASNVQVQTGYKCAVILRDLITPYLLRRLKSDVAQDLPKKNEMVLFVKLTRIQQDLYEKFLHSEELNSILKGKRNVLMGVDMLRKICNHPDLINREILMYKKGYNYGNPAKSGKMQVLKNLLQLWQSQNHKTLLFCQTRQMLDILEKFVANLHVLDEEGNDSTGYFNYLRMDGGTPISKRQTLVDKFNTDLTQHVFLLTTKVGGLGVNLTGADRVIIYDPDWNPSTDIQARERAWRLGQKRDITIYRLMTTGSIEEKIYHRQIFKTFLTNKILKDPKQRRFFKINDLHDLFTLGDQNEQGTETGDLFSSSEVKFSGTKPRKQTSLFKKKYTNDDDFYQVAKISGVAKLDQYVDEKEEPEQGKDEDRIMQGLFTNSGVHSTLKHDDIIDSSMQETSLIEREANRIATAAAEALKRSRKLARKKEIGTPTWTGKFGLAGKFGSKKKTKTSSSSILDSLKKKSEPTIYKPKVQLLDRDVIIQKLVDWLSKQEDKFSTSNDIMSSLDLKLSSEKDVILVRSMLREIASWDSIRKGWKLKEDFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJF4</accession>
    <name>G3AJF4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_60047</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374197.1">
        <property type="nucleotide sequence ID" value="XM_007374135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJF4"/>
    <dbReference type="GeneID" id="18873196"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60047"/>
    <dbReference type="eggNOG" id="ENOG502SVW2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042187_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJF4"/>
    <dbReference type="OMA" id="INIMTAN"/>
    <dbReference type="OrthoDB" id="3989662at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="395"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="501" mass="56211" checksum="EBBA1B57732BBFA9" modified="2011-11-16" version="1">MGSTLLHPALSEPESSPLSPANEPVDDEQSNLDDNKQSFALCKLLNSHSSVFNALNNPELDSPMLFDSPRLRTPEVFLDQSTTQGQQSQFSITSWQSITNEFLQNSPAISRVPSNIKRVTKTSSILSTDDDLPRDYSAIIVPSPSNINAMVFDTDEEGDSEYEIPQPSFIMPKMSFSANSEPAFNKYQVAILSFSHKPEIDQLIGSLRDELSKQVNIIHINLQGEWRNTKSIMQTSNLIFIINDGSSSIVKYLNTIMDKPVEEDSQKVTIVNLITVNYFINLFELINSLRPYQIWKAASLKQDNLIDRFTSFIELECDAEDNSKQIVPKAMYYSSLISTQRQDYKVIEKQFKFDLQSSTTYNDPFKLSKSFVKLDLLFSNFKKLFVSNGGDNNMWLIYSFTMGIGFGIGMATGTMSLLGYYVYDELCGGGKIETEAILQEAPKVEFDSGSTMDKLLEGLSNARIVLEVKEFCTNYVLGFSSTVFVCIQGGLEKVISLFLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJJ2</accession>
    <name>G3AJJ2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_59274</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373479.1">
        <property type="nucleotide sequence ID" value="XM_007373417.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJJ2"/>
    <dbReference type="GeneID" id="18872845"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59274"/>
    <dbReference type="HOGENOM" id="CLU_2819711_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJJ2"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="67" mass="7404" checksum="E0D98AB521276A8D" modified="2011-11-16" version="1" fragment="single">DVMTHANTKAQPMSRISLPNDVCRSDVARTQISQSQTAILSTCYSHLVCLYGGILTRATFFVSGFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJW5</accession>
    <name>G3AJW5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cell wall protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_65205</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373600.1">
        <property type="nucleotide sequence ID" value="XM_007373538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJW5"/>
    <dbReference type="GeneID" id="18875027"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65205"/>
    <dbReference type="HOGENOM" id="CLU_1504358_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJW5"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442373" description="Cell wall protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="191" mass="20576" checksum="5AA506BB06502374" modified="2011-11-16" version="1">MRFLPVLLVSFAALTSAANSTSTDDSVTDFNYQEFLNGLTKCDFDCYGVAVELAGCLPKGEEGESSTTSSDLQDTTDKGMKKMYKCLCEQDDDFFDKYSKCAKNCPIFSEIIDDDEKDAKKMKQFYCEGATATSELAKTSSSLDGLKSTTASATDTSEEQSNSTSDSTETKENGTGVFGVSLISLLAIALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK50</accession>
    <name>G3AK50_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_65885</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374376.1">
        <property type="nucleotide sequence ID" value="XM_007374314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK50"/>
    <dbReference type="FunCoup" id="G3AK50">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK50"/>
    <dbReference type="GeneID" id="18875066"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65885"/>
    <dbReference type="eggNOG" id="KOG0252">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_12_1"/>
    <dbReference type="InParanoid" id="G3AK50"/>
    <dbReference type="OMA" id="RGPIFIL"/>
    <dbReference type="OrthoDB" id="2153661at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015169">
        <property type="term" value="F:glycerol-3-phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001406">
        <property type="term" value="F:glycerophosphodiester transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17364">
        <property type="entry name" value="MFS_PhT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000140">
        <property type="entry name" value="Putative MFS phospholipid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0843">Virulence</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="358"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="47"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="482"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="482"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="510" mass="55991" checksum="C071733361057E08" modified="2011-11-16" version="1">MSSDKSNKIVETISVATNDGHKKHFKYDSEARKARFEGGVKLRDALMILCAGFALISDGYQNNVMSMLNKVFALEYPKEYTASMSTQVSNASLVGTILGQVAIGLTSDYIGRKWSIVLATCFLIFGTIMCAASHGNTVNGMFWMLTIFRGVTGFGIGAEYPSSSVTASEAANQSVKRRGGAFILATNLPLSFGGPFALIIFLIVNRITGTHYDGLWRTMFAIGAFWPLSVFYFRLKMVTSELYTKSAIKQRVPYWLALKYFWPRLIGTTVAWFLYDFVTFPNGIFSAGIISNVIKDATNVERIAEWNLLLGVIALPGVFIGAYLNDVIGRKYTIMIGFSGYIIFGLIVGCAYEKIKGITALFIVFYGLMMSMGNLGPGNGMGLISSESFATPIRGTAYGLSAAIGKVGAVVGTKTFTPIQTNLGQKWTFIIAAICGLAGVLVTFIFVPHLREDDLLEQDIKFKNYLIDNGWEGKFGIKEYDAEHDEDLEGEEDSETDGKEDVVEHTVQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK82</accession>
    <name>G3AK82_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_151807</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375056.1">
        <property type="nucleotide sequence ID" value="XM_007374994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK82"/>
    <dbReference type="GeneID" id="18870906"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_151807"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_12_1"/>
    <dbReference type="InParanoid" id="G3AK82"/>
    <dbReference type="OMA" id="FNMMFAA"/>
    <dbReference type="OrthoDB" id="508119at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000313">
        <property type="entry name" value="MFS quinate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF8">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="225"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="432"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="498"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="52"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="586" mass="64208" checksum="0A36C0EB92596D94" modified="2011-11-16" version="1">MIQSINSIEDKELKQQVELEPISSPPPKKKFRWSIDKRDHPKQVFNSTLYLSIFVFGILGAARGYDEGNISGNITLPAFIARFGINDPTKSADYLANLTSNITSMVQLGSIGGAFIAMKTVDFFGRVRSLQIICLLWIVGSIIQITSSNLGQLYAGRIIEGIAIGHTTSIGPVYLAEVSPSPIRGLASCIFAGAVYIGVLIGYLANLGCVLNVADTVNGHINDNQWRYTLIPKILLASLIFIMSTILCVESPRWLLKVNKVDQAVKNLSKLRHMPEDHAYIVAEISDINEQVLAEKDAIANSSILGNLRDLVTNKSIAYRFFCIAGAAQVLGQWSGANAVTIYASELFSLAGIKGPVGKLKMSAILGVVKVISAYLGAFFIIDVFGRKRALYSGISLQMVSILYYAIFLTIVPQASQNGTVLTPSQTRASKGALAAIFLSGTGWTIGFNSVQYLLGSEIFPLHIRSFAQSLVMVLHFANQYGNSKALPKLMLALQPYGAFYFFVGVMVLGLVFAFLLPELQGRSLESVEEVFTLPWYKLRNCDKLVPDHSNVHEINYINSRGKLVYDTGKVDEIVLQPEQKAEKMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKA1</accession>
    <name>G3AKA1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Arylacetamide deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_50429</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375075.1">
        <property type="nucleotide sequence ID" value="XM_007375013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKA1"/>
    <dbReference type="FunCoup" id="G3AKA1">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKA1"/>
    <dbReference type="GeneID" id="18871441"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50429"/>
    <dbReference type="eggNOG" id="ENOG502SBSE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_713702_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKA1"/>
    <dbReference type="OMA" id="NEIHIMG"/>
    <dbReference type="OrthoDB" id="2152029at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019436">
        <property type="entry name" value="Say1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF31">
        <property type="entry name" value="STERYL ACETYL HYDROLASE MUG81-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10340">
        <property type="entry name" value="Say1_Mug180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="387" mass="43635" checksum="CE413AF238B49526" modified="2011-11-16" version="1">MLSTVARILWLPYVGIKSVVQFYTTGTIYSVTNQEFEDSLYKNVHLAIIYHMSREITKFESKYLIHKPITSIFSQYRNNPIAQGLTNFGTKFDDNGYWVHQIPSSQSKKVLIYLHGGGYQLNMTDSQLLWAATMHYAIPKELANQVSILAVDYSLSMFDHVYPTQLWETLKVYKHLVESGYNEIHIMGDSCGAHLALSVARAIAYPDEAAEQFSHFPKFPFDFSQRLPQPKSLLLDSPWVEPCNNVKLPCAHGVDTTGDLGSPTCTMGDNFIGDNSKELINNFLTFTNTNYNDHWAQVEPITNGKTVILVGEREVLRDGIDKFHHIINKGDNVAYYVEKGGIHAAIAYVETLDYMSKSGGQKVVDGNLGNKFGITLFAKYLQQFASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKB9</accession>
    <name>G3AKB9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_60921</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375093.1">
        <property type="nucleotide sequence ID" value="XM_007375031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKB9"/>
    <dbReference type="GeneID" id="18873634"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60921"/>
    <dbReference type="HOGENOM" id="CLU_2499295_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKB9"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="86" mass="9750" checksum="0B305221D9A89350" modified="2011-11-16" version="1">MAPSFASSRKPIPNEGADLTNQRHLDRLAQSKLKQEQEALELRKIADEEYRKYYSFSLNIVGIGLGAVLLISFAMIAYQIRHEVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKF5</accession>
    <name>G3AKF5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_60256</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374427.1">
        <property type="nucleotide sequence ID" value="XM_007374365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKF5"/>
    <dbReference type="GeneID" id="18873297"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60256"/>
    <dbReference type="HOGENOM" id="CLU_2874077_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKF5"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="64" mass="7108" checksum="333CA1216D4A7583" modified="2011-11-16" version="1" fragment="single">MPSQAQKSSPWLAFGEDEHAEDKIVNTPDFVPVAPPPPVQNHKQVILQSKDPSIESNLKEIFSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKH5</accession>
    <name>G3AKH5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GTP-binding protein YPT7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_60274</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374447.1">
        <property type="nucleotide sequence ID" value="XM_007374385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKH5"/>
    <dbReference type="FunCoup" id="G3AKH5">
        <property type="interactions" value="953"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKH5"/>
    <dbReference type="GeneID" id="18873310"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60274"/>
    <dbReference type="eggNOG" id="KOG0394">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_10_6_1"/>
    <dbReference type="InParanoid" id="G3AKH5"/>
    <dbReference type="OMA" id="TSWKDEF"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990816">
        <property type="term" value="C:vacuole-mitochondrion membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043254">
        <property type="term" value="P:regulation of protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032889">
        <property type="term" value="P:regulation of vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01862">
        <property type="entry name" value="Rab7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000086">
        <property type="entry name" value="Ras-related small GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF20">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB-7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="23712" checksum="87DCBC3280AF7B6C" modified="2011-11-16" version="1">MSSRKKTLLKVIILGDSGVGKTSLMQQFVNNKFSHQYKATIGADFLTKEIIIDNNKPVTLQIWDTAGQERFQSLGVAFYRGADCCVLCFDVTNEKSLNNLTSWKDEFLVQSNVSNPQDFPFIIIGNKIDVDDSKKIPSLQKKLHNITHNQLGGLNYPVFETSAKDSINVEAAFEVIAKMALQQEELNAASGGDVNDDYNDAINIHLDGESSGCGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKS1</accession>
    <name>G3AKS1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_60305</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374490.1">
        <property type="nucleotide sequence ID" value="XM_007374428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKS1"/>
    <dbReference type="STRING" id="619300.G3AKS1"/>
    <dbReference type="GeneID" id="18873332"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60305"/>
    <dbReference type="eggNOG" id="ENOG502QSNW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024518_2_1_1"/>
    <dbReference type="InParanoid" id="G3AKS1"/>
    <dbReference type="OMA" id="TNEYEHN"/>
    <dbReference type="OrthoDB" id="10255128at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002410">
        <property type="entry name" value="Peptidase_S33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051601">
        <property type="entry name" value="Serine_prot/Carboxylest_S33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248">
        <property type="entry name" value="2-SUCCINYL-6-HYDROXY-2,4-CYCLOHEXADIENE-1-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248:SF2">
        <property type="entry name" value="PROLYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00793">
        <property type="entry name" value="PROAMNOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="83"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="461" mass="52401" checksum="F8498E2A29A18421" modified="2011-11-16" version="1">MSYQIVDCFRNKEIVNQRVSFDLPLDYSQPKGMKIKVVVNITQKAEGVDLAAGTTQLLLPSSPKFIGYLQGGPGFPCQVPTYSGMTKVLLDKGYQIIYLDQRGTGLSTPLEGTTFKDLVTQRFGNFEVQSQLDFIMNFRADSIVEDLETVRKQLIPNDKISLLGQSYGGFCSFTYLSKYPDSLREVLITGGVPPVGFAVDQVYQATYERTTERNIHYYSKYPQDKQKVVDICKYLDANKVKLPNGGDLSVERFQQLGLGFGATGGTDRIHDIVTKFAYDLQLFNHPTYQILHTIETQSGFDTNVIYALFQEAIYCDGNIRSNWGADRTRYTSDNVSKYTLNSEQVYFTGEMVYKSMFDEYAELRQFKELAYALHENSTWSKLYDPEVLRTITWDKVPIVAATYVYDQYVDFELTRKVKQNVIAIGNLKQYITSEYFHNGLRADPDKVLGVLFSLLEGGEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKU2</accession>
    <name>G3AKU2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Replication factor C subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_60975</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375157.1">
        <property type="nucleotide sequence ID" value="XM_007375095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKU2"/>
    <dbReference type="FunCoup" id="G3AKU2">
        <property type="interactions" value="855"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKU2"/>
    <dbReference type="GeneID" id="18873665"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60975"/>
    <dbReference type="eggNOG" id="KOG2035">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042324_5_0_1"/>
    <dbReference type="InParanoid" id="G3AKU2"/>
    <dbReference type="OMA" id="LKADIMH"/>
    <dbReference type="OrthoDB" id="761538at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000030">
        <property type="entry name" value="replication factor C subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000002">
        <property type="entry name" value="Replication factor C subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000136">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF1">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13177">
        <property type="entry name" value="DNA_pol3_delta2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22534">
        <property type="entry name" value="RFC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="34"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00070185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="360" mass="40680" checksum="326588F8CDF58C74" modified="2011-11-16" version="1">MSLWVDKYRPKTLDQLLYHDSITQSLKALSKSGDFPHLLIYGPSGAGKKTRIYCTLNEIFGSSVEKLKIDVKTFVTSSNRKLEFNVLSSPYHLEITPSDMGNNDRVVIQDLLKDIASTEQVDFNNQSSSKTKHRFKIVIINEADSLSRDAQAALRRTMEKYSSNIRLILVCNSISNIIAPIKSRTLLVRIPSPSITDINSILSNISVKEGIKFSSNDLEPFYTKVSTASNRNLRRALLSFETLTMQNETIKPETNLSIINLDWEVIIKNIANNIKTARNVATLAKIRTVFYELLSHCIPARIILKTLLFNLIEIIPDVRVVQELVNLASVFDERLSLGQKSIFHLEGFVAKSMVTIDQYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL19</accession>
    <name>G3AL19_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP synthase subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_150660</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit b, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. Part of the complex F(0) domain. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. In yeast, the dimeric form of ATP synthase consists of 17 polypeptides: alpha, beta, gamma, delta, epsilon, 4 (B), 5 (OSCP), 6 (A), 8, 9 (C), d, E (Tim11), f, g, h, i/j and k.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the eukaryotic ATPase B chain family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374576.1">
        <property type="nucleotide sequence ID" value="XM_007374514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL19"/>
    <dbReference type="FunCoup" id="G3AL19">
        <property type="interactions" value="474"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL19"/>
    <dbReference type="GeneID" id="18870873"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_150660"/>
    <dbReference type="eggNOG" id="KOG3976">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077208_0_0_1"/>
    <dbReference type="InParanoid" id="G3AL19"/>
    <dbReference type="OMA" id="YTEWADG"/>
    <dbReference type="OrthoDB" id="427795at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2210:FF:000002">
        <property type="entry name" value="ATP synthase subunit 4 mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008688">
        <property type="entry name" value="ATP_synth_Bsub_B/MI25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013837">
        <property type="entry name" value="ATP_synth_F0_suB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12733:SF3">
        <property type="entry name" value="ATP SYNTHASE F(0) COMPLEX SUBUNIT B1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12733">
        <property type="entry name" value="MITOCHONDRIAL ATP SYNTHASE B CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05405">
        <property type="entry name" value="Mt_ATP-synt_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161060">
        <property type="entry name" value="ATP synthase B chain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0138">CF(0)</keyword>
    <keyword evidence="3 8" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="234" mass="25717" checksum="BF7F9AE001B78278" modified="2011-11-16" version="1">MSMINRIALRAARPMGLRMAPYVLIGLRFASTQPVEPKQKAASIIDALPGNNLLSKTGVLATSTAAAVYGISNGLIVIHDETILLGTFSAFVLLCVKYIAPLYTEWADGEIKKVGDILNASRVKHVESVNDRIATVSQLKNVVETTQDLFALSKETAKLEADAFVLKQQLAVAHEAKSVLDSWVRFEAQQRQLEQEQLAKSVIEKVNKAIEDPKFQDKVLAESLAEVERLFAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALR5</accession>
    <name>G3ALR5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_66285</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374823.1">
        <property type="nucleotide sequence ID" value="XM_007374761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALR5"/>
    <dbReference type="FunCoup" id="G3ALR5">
        <property type="interactions" value="150"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALR5"/>
    <dbReference type="GeneID" id="18875096"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66285"/>
    <dbReference type="eggNOG" id="ENOG502RQ5G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_602674_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALR5"/>
    <dbReference type="OMA" id="DCLGQSE"/>
    <dbReference type="OrthoDB" id="5394106at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="248"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="386"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="68"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="264"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="294"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="392"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="405"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="470" mass="53273" checksum="EEDEBBED951FAD6D" modified="2011-11-16" version="1">MARSSLIPERPQVVSSRSRYSTGGFEFSNNTRSARSPASASQPLTNTESSPEVVETLRHQNRLLAQRNAMIMSKLKELELKNIELSKEINLLKRTTTPPTTDYKQALQEALLLIENKTFTSLDQILQCFKKIRSDEGLPANDQLGILSDVISRPVTSTPVEVGRNSSVFPAFDITSHHDPLLNYFKPTKEDYEAGTDNTAKNKDISIIFEDNESIEMGSESTDLNHSEHILPNEKEKQLEPSKVAIIKTPIESSSKTTEIEIYESPKEKHEYKTPSQTEVTCLKGKRKSKKTKTTKEKIQEDSKQQEPKHNEETPNEKKVAKTTRKKPPKEDSEPLVEVKQEEECSTRRSFRVRKPIDYKPLSMRDKMRRESVAMLDAVGPDVLINYTQKPSSRGSSSGSASSKRKSETPEVERKKRKPLATVNTNVHKGGSSKVKDTTDYDLSVYDFEPEDKKETPVYDKINVKNRIRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM54</accession>
    <name>G3AM54_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histone-lysine N-methyltransferase, H3 lysine-36 specific</fullName>
            <ecNumber evidence="4">2.1.1.359</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">SET domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">SPAPADRAFT_54781</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Histone methyltransferase that trimethylates histone H3 'Lys-36' forming H3K36me3. Involved in transcription elongation as well as in transcription repression.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60324"/>
            <dbReference type="Rhea" id="RHEA-COMP:9785"/>
            <dbReference type="Rhea" id="RHEA-COMP:15536"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.359"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
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        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.359" evidence="4"/>
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        <property type="protein sequence ID" value="EGW32759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_007374212.1"/>
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    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54781"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_008492_1_1_1"/>
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    <dbReference type="OMA" id="AQSQPCY"/>
    <dbReference type="OrthoDB" id="422362at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0140954">
        <property type="term" value="F:histone H3K36 dimethyltransferase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0009302">
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        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
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    <keyword evidence="8" id="KW-0489">Methyltransferase</keyword>
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    <keyword evidence="7" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
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        </location>
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            <begin position="509"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
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        </source>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="718" mass="82563" checksum="E393557B4D60556A" modified="2011-11-16" version="1">MSDPSRESTRTPPIKQPTPQIFLDAEDKTAEALSKFTELEACTYASKQLGSSQMQEFMTCDCEEEWDGELQLNLACGEDSHCINRVTSVECMNRHCLCGNDCQNQRFQKREYAAVSVFQTELKGYGLRADLEIGEGQFIYEYTGEVIDEATFRQRMVEYDQKSFKHFYFMMLKSDSFIDATVRGSLARFVNHSCNPNAYVDKWVVGDKLRMGIFAKRKIARGEEITFDYNVDRYGAQSQPCYCGEPNCIKFMGGKTQTDAALLLPEGIADALGVSSKEEKQWLKENKHLRQQQSDDAVINEMFIKQLEITPLTESDVSKVMGALMKSQDALIIESLIQRIYLTDDKSLNVLIIKFHGYKTLSNILEKTSDEKLIQTILEILSKWPKVTRNKISSSQIEEVIKEIERKYDNETIKSLASDLLHEWSKLKMAYRIPKAKPNETPSYSRVSRSPEREEETPQSPSLDPTPSEEPLPDGWEVAYDPNTKNNYYYHRALQISRWDRPVSSVPRGPKPPTGPRPPTGPGGYKRGGSSTPTKFSEADLAEREERRMQMLKNEQYRALQEKERQLQELIEQSRELERKKQEEQQQSQQKPEKKQKTVKSSHKHDDVSVEAKWKHVLAKHVPNLIKKYEKEIGRDNLKGCARDLVNSLASKEVKIDPKSSPPKELDHHKLKKIKEFSKTFMEKFLVKYRQKHDKKRSRDSDESGSDSKRTKVATNGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM90</accession>
    <name>G3AM90_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chitin biosynthesis protein CHS5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_50273</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CHS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374903.1">
        <property type="nucleotide sequence ID" value="XM_007374841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM90"/>
    <dbReference type="FunCoup" id="G3AM90">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM90"/>
    <dbReference type="GeneID" id="18871424"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50273"/>
    <dbReference type="eggNOG" id="KOG1181">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019904_3_2_1"/>
    <dbReference type="InParanoid" id="G3AM90"/>
    <dbReference type="OMA" id="TPYEFQL"/>
    <dbReference type="OrthoDB" id="245697at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034044">
        <property type="term" value="C:exomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000282">
        <property type="term" value="P:cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000747">
        <property type="term" value="P:conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="BRCT_CHS5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13945">
        <property type="entry name" value="Chs5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000077">
        <property type="entry name" value="Chitin biosynthesis protein CHS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.120.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031673">
        <property type="entry name" value="Chs5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052827">
        <property type="entry name" value="CHS_Export/Cell_Fusion_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031669">
        <property type="entry name" value="Fn3_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47351">
        <property type="entry name" value="CHITIN BIOSYNTHESIS PROTEIN CHS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47351:SF1">
        <property type="entry name" value="CHITIN BIOSYNTHESIS PROTEIN CHS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16892">
        <property type="entry name" value="CHS5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16893">
        <property type="entry name" value="fn3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="6">
        <location>
            <begin position="164"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="351"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="395"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="416"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="428"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="500"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="524"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="537"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00060872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00071189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="549" mass="60741" checksum="B8486DEE6CF2B968" modified="2011-11-16" version="1">MVEVSLTIGKLDASLALLLTKDHHLIEFPTILLPNGVKAGSIVKIRCDQDLKSEQEEEVHFQSIQDEILSTFATNLPKKPVLRVKNVTQTSCVLEWDQLDLGTSSLKNLILFKDGKKLGSIPSPLTNKTSKLSGLPIDKSFKFQLRLDTTAGTYLSDEIEVNTHKMTDLSGITVCLGDFSANDPFTKEDIEEALKTMGAKFPPQEQVKVDTTHFLCTRENKQNPEYVKANEMNIPIIRPEWLKACERERRIVGVRDFYVKDCVLPDIFAKNYWKNTSKQVSPQEEKAEPELPTIPAVEEVQETTKADDEVPPAEEAAEIAEVPIESVPEIEITQSDEKLAEDIENTQQNIDDAFGEPTEEEVKKDKPVESVEVSESEPVAEAEKEVVLEEIAIEQPEIVAESTPVEEVQASTNEVANEEPQVEEVALDEPKVAAETAESHDATTSEEPAKEELQEVSLVEPVAVSAEPKVETEESVVDESVVQDITSPENDEVAGKDNEEVGQDEEDEDNADEPEEGEGEGDAGNDTTATPAATAKDKKKKKKKKNKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMH5</accession>
    <name>G3AMH5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TUG ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_54832</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374342.1">
        <property type="nucleotide sequence ID" value="XM_007374280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMH5"/>
    <dbReference type="STRING" id="619300.G3AMH5"/>
    <dbReference type="GeneID" id="18871894"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54832"/>
    <dbReference type="eggNOG" id="ENOG502S0EM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_521749_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMH5"/>
    <dbReference type="OMA" id="ERIMLIW"/>
    <dbReference type="OrthoDB" id="440781at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021569">
        <property type="entry name" value="TUG-UBL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46467">
        <property type="entry name" value="TETHER CONTAINING UBX DOMAIN FOR GLUT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46467:SF1">
        <property type="entry name" value="TETHER CONTAINING UBX DOMAIN FOR GLUT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11470">
        <property type="entry name" value="TUG-UBL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TUG ubiquitin-like" evidence="2">
        <location>
            <begin position="8"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="439"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="445"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="483" mass="54406" checksum="BCCA88D5075F1B3E" modified="2011-11-16" version="1">MASIALNITYGASSKKFTIPKSTTISQLVSEALPKFGISKDEYTGTLSYNSKPLDSSLPIRLTPLLNNSKLKLSVSKLDLDFDVNVKLTVDGASSIKKCGASLTVRELLDLFAVDLTRDSEVRILDLKLTSSSGQYDSVKLGSVVTKKVGSVVIRLNYTKSVSEQEKINLEQQEAVKLQLEGERKRKLEEAERRRQEEEKANTERLLKEQKQEQEEKTAHVEPMDIDEEKPLQQFHDHKKVLEDVDSATTPSKQSTPIDPVYVEEPLAETPQLYVPSTSSKVSTYENPDEDYEMTLGQAKAYHNIVKNSGVRKKKVSPSPASLPSKYLIRVKFPDLTILQINFLEDVHLVKFGQLVKRIDELLYPDFINGYNLKLGYPPFSPLGQSFDMNSKLLHEMPDFKSERITLIWELAKNDLNISGPYVKSEGDVTVRATTEMPGQVLESMRGELPDDEQEKKPPKEVTGQASSESKKPNKLPKWLKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMI2</accession>
    <name>G3AMI2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoserine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">O-phosphoserine phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_60783</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily. SerB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.3" evidence="4"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374942.1">
        <property type="nucleotide sequence ID" value="XM_007374880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMI2"/>
    <dbReference type="FunCoup" id="G3AMI2">
        <property type="interactions" value="552"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMI2"/>
    <dbReference type="GeneID" id="18873567"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60783"/>
    <dbReference type="eggNOG" id="KOG1615">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036368_4_0_1"/>
    <dbReference type="InParanoid" id="G3AMI2"/>
    <dbReference type="OMA" id="LSMFKHA"/>
    <dbReference type="OrthoDB" id="27226at2759"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00198"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036424">
        <property type="term" value="F:L-phosphoserine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000143">
        <property type="entry name" value="Phosphoserine phosphatase serb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050582">
        <property type="entry name" value="HAD-like_SerB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004469">
        <property type="entry name" value="PSP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00338">
        <property type="entry name" value="serB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344:SF2">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01137">
        <property type="entry name" value="C1.6.1:_Phosphoserine_Phosphat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01136">
        <property type="entry name" value="C1.6:_Phosphoserine_Phosphatas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00029">
        <property type="entry name" value="phosphoserine_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604469-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW33427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="305" mass="33755" checksum="F6C3D0F5AB29259D" modified="2011-11-16" version="1">MSTTLTVISHFHTLSQVVLDEITHTLLIDQLSIESYTDKRLSPRAHDFYFDISDLNKARSTIKTFSSSTGRPYDIIIQPSATRQNKKLFVFDMDSTLIYQEVIELIAAYADIEDKVAEITTRAMNGELDFNQSLAERVALLKGIDATNLWEELKEKIVITKGVRELTKALKRLGVTMAVLSGGFIPLAEYVKGELGLDYAYANTLGVDEHKRLDGTTIGTIVNGDRKAELLLEIAGKHGIDPKDAVAVGDGANDLKMMRTGGFGIAWNAKPKVQLEAPACLNTDSLQDVLYILGYTDEEINQLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AML5</accession>
    <name>G3AML5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 12">Ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="2 12">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_60812</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 12">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the ribonucleoside diphosphate reductase large chain family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="2 12"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374974.1">
        <property type="nucleotide sequence ID" value="XM_007374912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AML5"/>
    <dbReference type="FunCoup" id="G3AML5">
        <property type="interactions" value="1284"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AML5"/>
    <dbReference type="GeneID" id="18873582"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60812"/>
    <dbReference type="eggNOG" id="KOG1112">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000404_1_0_1"/>
    <dbReference type="InParanoid" id="G3AML5"/>
    <dbReference type="OMA" id="IELPQHM"/>
    <dbReference type="OrthoDB" id="3000483at2759"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005971">
        <property type="term" value="C:ribonucleoside-diphosphate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051063">
        <property type="term" value="F:CDP reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046704">
        <property type="term" value="P:CDP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006240">
        <property type="term" value="P:dCDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01679">
        <property type="entry name" value="RNR_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.70.20:FF:000001">
        <property type="entry name" value="Ribonucleoside-diphosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013346">
        <property type="entry name" value="NrdE_NrdA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039718">
        <property type="entry name" value="Rrm1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02506">
        <property type="entry name" value="NrdE_NrdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573:SF6">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="R1 subunit of ribonucleotide reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00089">
        <property type="entry name" value="RIBORED_LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="13">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW33459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="845" mass="95457" checksum="E4E971CD442A3A25" modified="2011-11-16" version="1">MYVYKRDGRKEPVRFDKITARVQRLCYGLDPQHVDAVAITQRVISGVYQGVTTIELDNLAAETAAYMTTIHPDYAILAARIAVSNLHKQTIKQYSQVSKELYEYINPKTGLHSPMISKETYDIIVKNADELNSAIVYDRDFSYNYFGFKTLERSYLLRINGKVAERPQHLLMRVAIGIHGDDIPRVIESYNMMSQKYFTHGSPTLFNAGTPSPQMSSCFLVAMKEDSIDGIYDTLKTCALISKSAGGIGLHIHNIRATGSYIAGTNGTSNGIIPMVRVFNNTARYVDQGGNKRPGAFALYLEPWHADIFDFIDIRKNSGKEEIRARDLFPALWIPDLFMKRVEQNGEWTLFSPNEAPGLPDVYGDEFEELYERYEREGRGVKTVKAQKLWYSILEAQTETGTPFMLYKDACNKKSNQKNLGIIKSSNLCCEIVEYSAPDEVAVCNLASIALPSFVEKDENHSWYNFEKLHEVTKVVTRNLNRVIDRNYYPVPEARNSNMRNRPVALGVQGLADAFMALRLPFDSQEARELNIQIFETIYHAAVESSVELAQEEGPYPTYEGSPASKGLLQFDLWERKPTELWDWDTLKQKIAKHGLRNSLLVAPMPTASTSQILGNNECFEPYTSNIYSRRVLAGEFQIVNPFLLRDLVDLGIWNEAMKSNIIANNGSIQSLPNVPDELKELYKTVWEISQKHIIDMAADRAAYIDQSQSLNIHIKEPTMGKLTSMHFYGWKKGLKTGMYYLRTQAASAAIQFTIDKKVADQAGKTVATLEKLNKRKYVSKITSGSESEFSSRATSTEPSSLDNSIQLKKRQLKNLKSTSTVPSSLLVLLTISNPVPCVSVRTFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMQ6</accession>
    <name>G3AMQ6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_136443</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375015.1">
        <property type="nucleotide sequence ID" value="XM_007374953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMQ6"/>
    <dbReference type="FunCoup" id="G3AMQ6">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="GeneID" id="18869972"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136443"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_3_1"/>
    <dbReference type="InParanoid" id="G3AMQ6"/>
    <dbReference type="OMA" id="QLMGWLT"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000061">
        <property type="entry name" value="MFS sugar transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF68">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="373"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="407"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="443"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="472"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="47"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="546" mass="60986" checksum="B31F5B83058CB9AD" modified="2011-11-16" version="1">MTSYNKDNSSLSQESVTSSGNNHLDSSDDSNTYTYLGMKGQRLHHAISFLAGMGFLLFGYDQGVMGSLLTLPTFRNTFESIDTTRYPDRATFQGFVIAIYEIGCLAGALSTMYIGDKIGRRKTIFIGCIIMIIGAIIQASSFSVGQLIVGRIVTGLGNGMNTATVPMWQSECAKSKDRGRLVMVQGALITGGICISYWIDFGFFFIKNSSVSWRFPIAFQIFFPLFILPFILRLPESPRWLLKTGDMQEAARVFSSLDDVPVNHPTIVYEVNEIAESLEKERSDATNKSKFRLLFIQGETKNFHRTCLAVWSQIMQQITGINLITYYAGTIFENYIGMSPLNSRILAAANGTEYFLASWIAFYTIERFGRRKLMLFGAAGQAASMAILTGTTWAASPKNENNSSAGVAAAVFLFIFNTFFAVGWLGMTWLYPAEITPLNIRAASNGLSTAANWSFNFMVVMITPIAFENIDYYTYTIFAVINLLMVPTVYYFYPETAGRSLEEMDKIFELSNTSTPWDVVKIARELPYESQTREGDIEKVAIEHIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMX4</accession>
    <name>G3AMX4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_55850</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375665.1">
        <property type="nucleotide sequence ID" value="XM_007375603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMX4"/>
    <dbReference type="FunCoup" id="G3AMX4">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="GeneID" id="18871979"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55850"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_27_4_1"/>
    <dbReference type="InParanoid" id="G3AMX4"/>
    <dbReference type="OMA" id="RIPYPML"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17476">
        <property type="entry name" value="MFS_Amf1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF1">
        <property type="entry name" value="LOW AFFINITY AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="350"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="381"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="411"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="444"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="488"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="57"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="539" mass="59074" checksum="78E9AAAB23D210D3" modified="2011-11-16" version="1">MPCINKLYQIATFNTSVSSCLNQRLHTNSARTLSRNFFFFPDQPNFEEISTFREILFMIFMCMSQLITQAAVAQTMNTSIQISETFNVGDQPGEISWFSAAFSMTVGTFILISGRIGDMYGYKKLFIIGYVWFGVFSLMCGFAGLTTSTVFFDIMRAFQGIGPAIMMPNTQALIGSFYPESKKKNICMACFGAVAPSGFILGALFSGLFTVKVWWAWSFWVCGIIGIALAVAAFWVIPKRIGVKSQGSFDYLGSIFGVSGLIFINFSFNQGPNVGWEKPYVYVLLIVGFLCIGVFYFVETKVKDPLVPPSVLKGDTGFILGCIAAGWSCFGIWLFYMFRWALLVDESGPIMAAVLNITTGPMGFIAAIGTAILLTKIPSAVVMFLAMIAFLVGSVLMGTRPVGQIYWAQKFVSLIIQPLGMDMSFPAACILLSSALPRRQQGIAGSLVSTFVNYSISIGLGFAGTVERYTTLHMEPGLPTTIHGMRNAFYMGMGLAGLGVVLSTVFIFIQLRNRSRNTIVEEESIKSESGGDSEVQPFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN98</accession>
    <name>G3AN98_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61548</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375757.1">
        <property type="nucleotide sequence ID" value="XM_007375695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN98"/>
    <dbReference type="GeneID" id="18873946"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61548"/>
    <dbReference type="eggNOG" id="ENOG502RZ25">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063516_1_0_1"/>
    <dbReference type="InParanoid" id="G3AN98"/>
    <dbReference type="OMA" id="IAREHCF"/>
    <dbReference type="OrthoDB" id="4096201at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="130"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="243" mass="27797" checksum="6E4908DEA8B4BB0B" modified="2011-11-16" version="1">MMVSDDEDDEEADNLDCKLLSGNTPISSSISLNIHGGDRNVVVDEDVDGKCNIPTSDKTSRSRCFEYLVGAIDEAWARYCDATTHVEDEVYGYDTPRSVATDEEDNFFDNTTDMTEYEDSDFENNHEKPQSQQQPLAQQQQVPPTLLQQQQPSFRRRMSSASSMSSKDDPSSCQLQELKNRLTKAKYYLQDLVDSDDYDEVASFWKRWDMIKYATIELVEDDDDDEIIESTIDELEAGRLFVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANA5</accession>
    <name>G3ANA5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAR1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61555</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375764.1">
        <property type="nucleotide sequence ID" value="XM_007375702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANA5"/>
    <dbReference type="STRING" id="619300.G3ANA5"/>
    <dbReference type="GeneID" id="18873950"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61555"/>
    <dbReference type="eggNOG" id="ENOG502S3H3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_977171_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANA5"/>
    <dbReference type="OrthoDB" id="410651at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="127"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="285" mass="31990" checksum="9088538EB43CCB9F" modified="2011-11-16" version="1">MLDPNTKTKKLKCPFSMTASFKKSTGVWTLRTTCNEHNHPQLDPLSNHPMLRKRSDELNLLILDLYKVGTKPSHIEAKIKEQYPDVLIKREDIYNEIRGYKRKLKKQTARFGQISSARLSNYRRRTQQLSSQQGQPQGDDAAAVAAVAAAAANANAFLHQDEGSEVLYDIPHHQIHHQVHHPSQQQLHQLQQQQQHAQQHQHSQQHSDEFQRQFNEASAAVESQYQQYQYDGEGNNNSGSSSSAVAIAAVANAARAAAGGSNGQQHYNNDDELSIDNIDSRLVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANB3</accession>
    <name>G3ANB3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61561</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375772.1">
        <property type="nucleotide sequence ID" value="XM_007375710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANB3"/>
    <dbReference type="FunCoup" id="G3ANB3">
        <property type="interactions" value="173"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANB3"/>
    <dbReference type="GeneID" id="18873955"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61561"/>
    <dbReference type="eggNOG" id="ENOG502RXWY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088025_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANB3"/>
    <dbReference type="OMA" id="LHKGPRV"/>
    <dbReference type="OrthoDB" id="2281895at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040030">
        <property type="entry name" value="Ribosomal_mL57"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28160">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L15, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28160:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14622">
        <property type="entry name" value="Ribonucleas_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase III" evidence="1">
        <location>
            <begin position="78"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="228" mass="25426" checksum="CF3A06C9EC663AB6" modified="2011-11-16" version="1">MYIRQLVTGTFRTVKLTPKLSPLTTTRSIYLHKGPRVAGLKRDPQEALVNSQGLKYELTDDNIKHIKTYLGDKYSIPDDIVLQVLTHKSFGNGIKPYNEKLAAMGSKLLSFYLAKHVTKSANKDSFAINGRNLDVLGSPISRELGGLFTLGVFAKQQGLNQVMFWTTRNANLGFESSGELRISAQMMYALIGAVNFYHGKEVAELFIQERLIDQLEKITVDLVSGKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANC7</accession>
    <name>G3ANC7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ras GTPase activating protein RasGAP/neurofibromin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_138165</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375786.1">
        <property type="nucleotide sequence ID" value="XM_007375724.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3ANC7">
        <property type="interactions" value="162"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANC7"/>
    <dbReference type="GeneID" id="18870100"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138165"/>
    <dbReference type="eggNOG" id="KOG1826">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000439_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANC7"/>
    <dbReference type="OMA" id="LPMGYHE"/>
    <dbReference type="OrthoDB" id="28245at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05392">
        <property type="entry name" value="RasGAP_Neurofibromin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF60">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN RASKOL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GAP" evidence="2">
        <location>
            <begin position="1286"/>
            <end position="1469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="2653" mass="305801" checksum="0CC50A3C451A0872" modified="2011-11-16" version="1">MSNPNDHQKLFIQSLAKRIESLLPNRTGHSTQEVELNSQFIVTRKILLDKYKRYPHIITFIFTSFATVLENINEESQHTKLRFRDDKSRGSTLLVCKLLSEILQTKWNRRGVIDDKDSLSNYSRFYYYDEPKALDKDIITLLLDTFSNLLSSGVVKKVLSLVRNEQSMASMATAKDEQDIQREDQPMTDEELIASSIVDIDSYIETILRYISTANPDDYYDYIYDKLFRYSRNDEIIPLPILQKYSPLIKFMFFSQENSERITEDVINAIPFIKSNTWRQVFLFFLSYSVKDQTFSRTKDYEGIVDPKNTRYANNLKSLFDTAGTAFDDSITSVTCASFVLTWFLVLCLDDFVEINTGRPINKLKLTFNKRVRYLMSILRDSANCTNLESFDALISIFHLGTRLNAHNITNHPVLIFSNKFLDQTYRNLLKFGDNHRDDLITDDELLVKFEYLTVNFYIAAIILRPERYTKIIIQNFNSNQEELREAKILVKIIKGLSEIETAQEIFYNLINQMLNQLVSLMYGSLKMMHQYDLTHPQHQQHCSYPTPVTDQSLFSDVASIECGQSLQKITNHTFSTRKTNLDHYVHEVLVEHSTSNESSASSTTNTNTTASKSIKLAHSTSSMSTHSAYKFRLVVGAEELLSDIFRIFCAAPELYFNDMVLMDDKNLEIRDYDQLIKEIMEFCYQMEIPLRYAFRGKCYIQGNTRLFDSARELSLKLVSKNTKIESNYTTLSAFANFFIVNCIVQSICETCLSLSLTDPKFKACFLFLNDVLIQRNQFTYILASNKVINDQARKESFSSFGEVIHAVEKVLLLSVCTHDIQFYNYAKEGMKWYINEMTIHHQLYRNIGVDENLLETFVNLSQDVSVFTGFISLHKRHRTILREAKPTKSLFQVWVLIYYRWLMILENKPSIINEENLIFRHFTGFLVSTSGCFMSNAGFKDKEIAEKSPAYMSEFFDKCVGLLNSSDLVVRVIIKDTLSNESHSDVYHLIATKLMTMANNYAERQQVTDEGVVFVEQAMVIMSSMVQVRNDGTLLLSALLPNICEFFIRFVNKVDNLTDKLRLKLRFCKLGTTLEQDRTSTGLNGAYKLRNFYAKASCEWLEDAVFYDEKRDVEHDSDSVVVKESEIAYLNMDLAVQSSRCLRGQLENLVLEIPEGIRDSDINKYKDLAFGNYFSLFYKIIQKYSKMNLSANKSKHKLNQISENILACITNLLQYDSDIGMQFVLPMGYHETAKVRAIFLNVFANMLSSKTMRGTRQEYPDEIIEELTSQTEIFGAIAESASSLEHNLLASSLFGIFCYSGRLDELFNVLLVDEISHLTRSTDLFRRNSTLTRLLFNFTQDYGMDYLNATLRPIIEELVDNQVIFEVEKSETINPDLFFQYFNKLVDSIVSSEDQLPASFKFVCGEIACAVGEKFKDASIIAVGSFMFLRYLCPAIISPEQFFKIPINHVKVKRSLMQLVKVLQNMANGTISSIKWPALTARMDELLTINTKIYDFLSRVSSVSTSEYPFQRSMERPVAELRYLHKFIYCYFVQIRIQFILAKSSFMVKTLHDRAEKFKAFDKTCMKLGQPKPSVKLQINSTLKMLDPNTANGESSIKFNDFMTKMSLKYAEVGDTSGDVIHSSIFKDGTPVVVVNFKKMKHRPDDTDYLVYKLFETASQVWDNRFYMVIDFTEFNLNHMTQEWAGGEGYVKLVNNFSTDQFFKNCARVYYYNIPRTGFATITDVIMRSRARSLEYGTKLYTYSQVDSDEIINRLCLDRETMAISKDNKVKFKNVQVYDPRNNKFVGATIKIGRKFLQICFDDRVLFSGDDIVTKGFTPVEIYRLSDFVKCEVSRITEHDDEFTIYLSLGTTVTFRSADRLEILRFLYYTTSRLPAETKYIDPDKDYQNERHTMHWFGRLYNIVFQGLLTNDDEVKSAAALLFGSLSSYFDIDFGIKENHAKSVSFPANTADFVVSVSDHLASSLSHMTYRFFKSFFDNYDKIPPANRLTSILYISPWIENVYDYVYLSTDENGPERVADLIRQFCRLSAMNKDHIAIIIDYIWKKLFSETRLISVLVDEVVAFAIDNKNEGPQWTFIIAIIIPSIEVCGEVINRLISCINRARTNDSAIVSQSKIFEIKVLIKICSSLFFNSYTLARLYLADLVFFVSLFIDNSNLDIGSDLQKLIIGLIQSFLHKPDLTEAEQKLVDDTIEYFSTLRAKMLFGMTRDSNSSLDISQSFNRITNFEVLCDYLNEFISAISSTDDKTNWKSRWCSNAIDVAFSKDSLFQDRAILIVGILSKSGISDSTACRTIKLVANGELPNLDFVICISVATSRILMGLPDTSILPPILIWPQFCFGLLNYTVLYQASIQNITTSVLKMIRVGPDYIQHSYQQRQLLEPHLSEFEKRHGFHITPTNFGAHIFFVLTQGLRFSQFKSLSLSCIKEYFTTRYQYRDRTVMDGAFISNNAYPYLVFTYLCSEQAEFEKYVEEIELATDYVQIGSCRIPKVIIDFLSQGTEASKVTLIHAGFFFNDIKIIDGEFKSKFLILYHHLFELNRENGFLIYDILKPALNNDLVTTTSIDVVDSISNIITEVSMCDDYVPEKYTKEVEEILVRNKIEALRSSRAMKNADDIVNNSKPGFRLDFFTDIKIIQTMLYRSACVYVEGLKLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AND3</accession>
    <name>G3AND3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proteasome assembly chaperone 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_55948</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375792.1">
        <property type="nucleotide sequence ID" value="XM_007375730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AND3"/>
    <dbReference type="STRING" id="619300.G3AND3"/>
    <dbReference type="GeneID" id="18871990"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55948"/>
    <dbReference type="eggNOG" id="ENOG502SCWT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1610525_0_0_1"/>
    <dbReference type="InParanoid" id="G3AND3"/>
    <dbReference type="OMA" id="FEKLMFI"/>
    <dbReference type="OrthoDB" id="3980246at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053720">
        <property type="entry name" value="Psm_Assembly_Chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018854">
        <property type="entry name" value="Psome_chaperone_3/4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10448">
        <property type="entry name" value="POC3_POC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="16075" checksum="995EE208EAF2F23C" modified="2011-11-16" version="1">MTITEDPTGFSKSFIAHFNDNDKTEFFFHKLTLADQQIINVQMDGVMDTTFDIPMTTQASINYASSMEIDGNTLGVPEPVLMIGDHANMKISIVASQIAKLIALRSPRHTCLSIGSRWFGTGDEVQDGDFEKLMFIIENIKKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AND9</accession>
    <name>G3AND9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_50540</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMC family. SMC4 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375204.1">
        <property type="nucleotide sequence ID" value="XM_007375142.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AND9">
        <property type="interactions" value="873"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AND9"/>
    <dbReference type="GeneID" id="18871454"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50540"/>
    <dbReference type="eggNOG" id="KOG0996">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001042_4_1_1"/>
    <dbReference type="InParanoid" id="G3AND9"/>
    <dbReference type="OMA" id="CPALDNM"/>
    <dbReference type="OrthoDB" id="5575062at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070550">
        <property type="term" value="P:rDNA chromatin condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070058">
        <property type="term" value="P:tRNA gene clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000481">
        <property type="entry name" value="Structural maintenance of chromosomes 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000585">
        <property type="entry name" value="Structural maintenance of chromosomes 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937:SF172">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="14">
        <location>
            <begin position="703"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="424"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="865"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1026"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1179"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="95"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW31928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1402" mass="159402" checksum="6E3B44B14D493679" modified="2011-11-16" version="1">MKRKRPAIPDSEDESTFINNSDYSASNNGHSPGPSVAAPGLKSEGDDTFMTAEYNQSDNTNMVFEHSDIITDGPKIQIERIPEEIENGLVDDASEPSNSDPETDPSTHMASIVNVQEPADVSAVSKQEEKTSFIASSPNHPPPVILPIISPTKKTRESLIVNSAIKSEPRLVIDKLRLTNFKSYAGEQIIGPFHSSFSAVVGPNGSGKSNVIDSMLFVFGFRASKMRQGKLSELIHNSEGFKPDFCQVDIYFHLVLDDPIDPQKSEIVPNSELIVSRKAFRNNSSSYYINGRTSNYTDVTTLLKEKGIDLDHKRFLILQGEVESIAQMKPKAEGNNDDGLLEYLEDIIGTTKYKKLIEDSMLRIDELNDVCMEKANRFDLVEKDKEQLESKKTEALRFLELEKKLTHAKSIQFQVNIFHHNKKIEHRQKEFDELNQELEQGREANKELLQGIEKEVDQQKKIEKEIKILTGQIDELVKHRKEITKRNVSNEEKVKNLNNKLKKIQKSLETSKHTLNSSNQKLSNYADAQAQFKSETEKLNNQLIEEDAKLQEIRQSLTEKTSEFTKEIEALQTKLEPWKNQLKEKDNAIQLAQSNIEILRTQMTSTTKQLDEAKEKLLTIKKEGKEKENEYRENEEKLEKIKEQIALGEEQCKVERNQVENMKSQLSSFRHKVQESSSIVSTNQNKNKVLTALLRLAKSGRIDGFYGRLGDLGLIDEKYDIAISTAGGPALDSMVVETVETAQSCIEYLRKNNLGYANFICLNKLRSFNLAPIQTPGDPAKVKRLFDLIQPTSSKFAPAFYSKLYNTLVAPDLAEAKRVAYGPKRYKVVTLDGKVVDISGAMTGGGNYPSKGGMRLTNSRGGTESMVSEKDLEEMKIRLNEMENKFEMASAAFEEKNSMLRKLKDLKPETEFAISRLRLDIESLASEKKEITQVCKNLIAEQQNMEESNPFEQQIKEKEQEVEKLTIERSELKSEMKVYEMQISTFEQKIMDAGGVELKMQSSKVDSLKNQISIIHEKTSGDRMTVKKLENDIKRHTKIIETATKEQEQAQADLNLIQASQGDVDSELQEINGRIKQLETERGDREDELETLKTELETKQDEINKFKSAEIELENKLEKVNSSIKKLHHLIEQDKEELEGIIPRDAQPYLYWLEEEEQAKYNGGIIEQLSEEEIRDVDLENVNATIEELEAYMANVKVDIEVLKEYGTKIVEYKERKNDLNQAVEERDVSKDYCEDMKRKRLDEFMVGFNTISMTLKDMYRMITMGGNAELELVDSLDPFSEGILFSVMPPKKSWKNISNLSGGEKTLSSLALVFALHTYKPTPLYVMDEIDAALDFRNVSIVANYIKERTKNAQFVVISLRNNMFELAQQLVGIYKVNNKTKSIALGNIDISNFSFGKVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANG2</accession>
    <name>G3ANG2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_61066</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375227.1">
        <property type="nucleotide sequence ID" value="XM_007375165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANG2"/>
    <dbReference type="FunCoup" id="G3ANG2">
        <property type="interactions" value="942"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANG2"/>
    <dbReference type="GeneID" id="18873702"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61066"/>
    <dbReference type="eggNOG" id="KOG0417">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_13_2_1"/>
    <dbReference type="InParanoid" id="G3ANG2"/>
    <dbReference type="OMA" id="AEPHEDN"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031371">
        <property type="term" value="C:ubiquitin conjugating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006301">
        <property type="term" value="P:DNA damage tolerance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010994">
        <property type="term" value="P:free ubiquitin chain polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044395">
        <property type="term" value="P:protein targeting to vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23813">
        <property type="entry name" value="UBCc_UBE2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000020">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="3"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="152" mass="17230" checksum="77681F3EDF08460C" modified="2011-11-16" version="1">MAALPKRIIKETERLVSDPVPGITAIPSEDNLRYFEVTIDGPNHSPYADGVFNLELYLPDDYPMCAPKVRFLTKIYHPNIDKLGRICLDVLKDNWSPALQIRTILLSIQALLGAPNPDDPLANDVADDWKKDEKRAIEIAKEWTLKYATKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANH6</accession>
    <name>G3ANH6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_139719</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375241.1">
        <property type="nucleotide sequence ID" value="XM_007375179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANH6"/>
    <dbReference type="STRING" id="619300.G3ANH6"/>
    <dbReference type="GeneID" id="18870230"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139719"/>
    <dbReference type="eggNOG" id="KOG2508">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017405_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANH6"/>
    <dbReference type="OMA" id="PASWWHE"/>
    <dbReference type="OrthoDB" id="415358at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF100">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="2">
        <location>
            <begin position="219"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="519" mass="59656" checksum="25BBE274FF8EF18D" modified="2011-11-16" version="1">MSEQPPPKKLKQGISSSYINYTISKGDKLAVLTELPSSGEFFQNFIAPRKPVKFKLTESIIPLDKFRLGNLESTLHYHHDLQIEKKYQSGFGSGQKRVKFGLGKLIEEVKSGNDEFYLTTQYDFDDPDAKSDEEEDNDEDEEEFGEEEEGDDDDDEEEQGENINFKFSDNFSETSSIDMNNLRDDFEEDFEEDSNTTDSILDEELSLTKSEALVRLKDMYQAPLTNLINAPDILPTIPPCFEKLIPQQINLWMGSSNISSKEFKLDESDKSGLGLGKQLPGDLTGSSSGLHHDHADNLYIVVQGKKRFTLYSPADAFKLYTVGTIYRLYDSGIIDYEVDKNAPHWRHIRDDGAIVEEIIRWKLDGLDDKDSKEKSKLLKQLQWEVKQRNEMKGVKTDLDPPNFSKIPPALLHLDEIDNKDIRNKLETFANEYFPGFLELNSFKVWLEPGDMLYLPAGWFHEVSSFGDDDNHGHIALNYWFIPPNTDNVETCYSDMYWHEDWAKTRRSLELAQNGKIELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANQ8</accession>
    <name>G3ANQ8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_71531</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the MATALPHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375269.1">
        <property type="nucleotide sequence ID" value="XM_007375207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANQ8"/>
    <dbReference type="FunCoup" id="G3ANQ8">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANQ8"/>
    <dbReference type="GeneID" id="18875383"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_71531"/>
    <dbReference type="eggNOG" id="ENOG502T26R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1408558_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANQ8"/>
    <dbReference type="OMA" id="PNKAIWN"/>
    <dbReference type="OrthoDB" id="5398665at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008301">
        <property type="term" value="F:DNA binding, bending"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045895">
        <property type="term" value="P:positive regulation of mating-type specific transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006856">
        <property type="entry name" value="MATalpha_HMGbox"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04769">
        <property type="entry name" value="MATalpha_HMGbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51325">
        <property type="entry name" value="ALPHA_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Alpha box" evidence="6">
        <location>
            <begin position="86"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="190" mass="22509" checksum="95336739823E5998" modified="2011-11-16" version="1">MKRRKTNKRSIPKGFISLFKIQRKRNVLRLDTSLAERSGNYEFRSMPLPKLPEPSEELKKVLLRYNIVKELAPLQEQKRKKLRPKLKLKPINSFMAFRSFYTKSISNPEYQRELSCLLAQIWIDEPNKAIWNQYTESYNQYILSSGAKYNFVNWLCKMLDIEAENNNTDLSNRANETILSGTVEDIYMFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3APL6</accession>
    <name>G3APL6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_152768</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375463.1">
        <property type="nucleotide sequence ID" value="XM_007375401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APL6"/>
    <dbReference type="STRING" id="619300.G3APL6"/>
    <dbReference type="GeneID" id="18870936"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_152768"/>
    <dbReference type="eggNOG" id="ENOG502RISH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022727_0_0_1"/>
    <dbReference type="InParanoid" id="G3APL6"/>
    <dbReference type="OMA" id="SGRIHTW"/>
    <dbReference type="OrthoDB" id="843225at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="656"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="485"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="658"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="707" mass="78437" checksum="1C2179AD8633AEB3" modified="2011-11-16" version="1">MPSLTSNGGGTDSPSLSPTTSNVESTPNGSSRVPPVPPIIVLSGASGKNTPVDELKAESLPHHLVQTHSDSELITLKQSSKRPTKLNRARTDSDDEVNSDDDNDDDDDDLLDYLKHRQSDDYKYIKFPILEQEEPPSKFFHNSVSFDDINLRCDTDDEPEWNDDLGWDLFRLHNAKGKNVKGYSNNYYYSSTRGRDSLRSPSPGGGTSPLASPTRRNSSPENLVGSSSISGVFDAVDGVPGGFIEYPSQPITQRKCCSISRQHVLYEALYKGKLPFLPKLPNRSILVYVSGRRHTWVALDWILTKFIENGDKVIIVSSVNADILERRRKRGRKGSTFTSPPKFGSTTAPLMRVRQRTVPHNIRNIASDIMDYVLTIIDPKVIAKVVVEIVAGKTKEVLKDLYRLYEPNLVCTGTKPNIKTGAPLKSWTSSKLTDRLVKNFPLPVIVVPAVNMSQFELELQSRFVDAIPEEPHHALSPKLEESVGDDSSINSCGSSGDPSSQNDQTDQSSAGSYSSYDEISNLYINYKRDVESELDQARELPVSANYYANLAKIISDKSSSLCSDIVDVSPDFRGNGSKLARAITGSNSFGVVPFRTKSLLGPVEDTTPKLSFKEVKEQLKKNKLQDDAQYLKPRATSLKFADDVKPNVINKKNLGKIRSHEVTSARPKLEPRKSHPGELKLSQSEDMLVTEKTKKSKKKGFWKSLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3APN4</accession>
    <name>G3APN4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phytanoyl-CoA dioxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_61288</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375481.1">
        <property type="nucleotide sequence ID" value="XM_007375419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APN4"/>
    <dbReference type="STRING" id="619300.G3APN4"/>
    <dbReference type="GeneID" id="18873820"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61288"/>
    <dbReference type="eggNOG" id="ENOG502RXJG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056749_0_0_1"/>
    <dbReference type="InParanoid" id="G3APN4"/>
    <dbReference type="OMA" id="SFARCYF"/>
    <dbReference type="OrthoDB" id="2106152at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008775">
        <property type="entry name" value="Phytyl_CoA_dOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40470">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_2G15850)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40470:SF1">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_2G15850)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05721">
        <property type="entry name" value="PhyH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="276" mass="31855" checksum="35321D51B87D4CB5" modified="2011-11-16" version="1" fragment="single">MPTVLPHKYHDEIEDQGFTVIRGFLNPEEITKYLQASENLVQYAREGNWPHVRTRGKQFPPWPKNFSPDIWGVSGLLHPDLNELLRPFHDLYADEKMLNVVEDILQVEKSGLSMELLNMLINPLTDFDLDWHRDTIKPEVSPEQEAEELLVNPYAGAQFNLALTDDSCLIVVPGSHKRLRTEEERDKTVNESKRKEYITNQLVVELHPGDLAFYNNNILHRASYIAKNKRITLHGSYGNVELGKFRAKGILQHGVAEWLPRLQTDNENLAMLKEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ16</accession>
    <name>G3AQ16_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <ecNumber evidence="3 9">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ZWF1</name>
        <name evidence="12" type="ORF">SPAPADRAFT_61414</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="3 9"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375613.1">
        <property type="nucleotide sequence ID" value="XM_007375551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ16"/>
    <dbReference type="FunCoup" id="G3AQ16">
        <property type="interactions" value="524"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ16"/>
    <dbReference type="GeneID" id="18873881"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61414"/>
    <dbReference type="eggNOG" id="KOG0563">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013524_2_3_1"/>
    <dbReference type="InParanoid" id="G3AQ16"/>
    <dbReference type="OMA" id="ERAGYYE"/>
    <dbReference type="OrthoDB" id="60984at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00408"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000015">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF0">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000110">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 9" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="6 9" id="KW-0521">NADP</keyword>
    <keyword evidence="7 9 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="10">
        <location>
            <begin position="13"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase C-terminal" evidence="11">
        <location>
            <begin position="189"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="498" mass="56876" checksum="84ABB96AE2E74AF4" modified="2011-11-16" version="1">MSYHSFGDYTTIVVFGASGDLAKKKTFPALFGLFREGHLPKTIQIIGYARSKLEDDDFKDRISKNFKGATEETKKEFLSLVSYISDPYDTDEGYIKLRKACESYEEKHGVKNPHRLFYLALPPSVFTVVCKQLKKEVYAGETRIIVEKPFGHDLESARALQRDLAPLFTEEELYRIDHYLGKEMVKNLLVLRFGNTMFSGIWNKSNIASIQISFKEAFGTEGRGGYFDSIGIVRDVMQNHLLQVLTLLTMDRPVSFDPEAVRDEKVKILKAIEPIDTEDLLLGQYTKSEDGTKPGYLDDDTVKPDSKCVTYAALGLSISNERWDGVPIVLRAGKALDESKVEIRIQFKPVAKGMFKEIQRNELVIRIQPNEAIYLKINSKIPGISTETSLTELDLTYSTRYSKDFWIPEAYEALIRDCYLGNHSNFVRDDELDESWKLFTPLLKHIESSENDIPLVKYAYGSKGPDHLRKYLEKHNYVFDEPGSYQWPLTSPSVKGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ85</accession>
    <name>G3AQ85_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_61995</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376210.1">
        <property type="nucleotide sequence ID" value="XM_007376148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ85"/>
    <dbReference type="STRING" id="619300.G3AQ85"/>
    <dbReference type="GeneID" id="18874141"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61995"/>
    <dbReference type="eggNOG" id="KOG2118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_553387_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQ85"/>
    <dbReference type="OrthoDB" id="5353557at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010960">
        <property type="term" value="P:magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000006">
        <property type="entry name" value="DUF21 and CBS domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045095">
        <property type="entry name" value="ACDP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064">
        <property type="entry name" value="METAL TRANSPORTER CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064:SF97">
        <property type="entry name" value="METAL TRANSPORTER CNNM-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="CBS" evidence="8">
        <location>
            <begin position="60"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="163"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="280"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="446"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="163"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="185"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="304"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="322"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="376"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="446"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="493" mass="54875" checksum="F955A8D4B9111DA8" modified="2011-11-16" version="1">MTPMDRVYTMSADTILDEKTVEEIFNAGFSRIPIHLPNEPNNFIGMLLVRVLISYDPEDALPVAAFPLATLPETALDTSCLNILNYFQEGKSHMIVVSEHPGEPVGARGVLTLEDVIEELIGEEIVDESDVYIDINKKIKRKQPGPLSKRNLTSYLHTLYQRSAQNSKRNSLDSSYQPDLRERLSQPQINNVIQTTPKDNVKPKNPAQNPLETNKKFVTIKKPVDQNTLDNVTKAVSPVPFGSSSATPAPASTLQPSQDAYGAVYSTEKGALVEAQRRSFEGERYSHTHEDKRDLPHYHVPNIGSRTASSSSVKKNPARFLLPEERETDVSEIHEESHGRQEHEHGDHHDDHEQDGIKHYHGSPDHSLVSPVGIPTTETSSAAGLAIPQSQESRSYRTGGIIESVVNVQGVHKTIIENVSDEELQYEQMLHERQLAKKNFSTDDIDNAIQDGDEESEGLLNNGRRHSSGTNGNGRRMSLWEKIRGASQSPSEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQG3</accession>
    <name>G3AQG3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxidation resistance protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_62089</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376288.1">
        <property type="nucleotide sequence ID" value="XM_007376226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQG3"/>
    <dbReference type="FunCoup" id="G3AQG3">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQG3"/>
    <dbReference type="GeneID" id="18874181"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62089"/>
    <dbReference type="eggNOG" id="KOG2372">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029204_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQG3"/>
    <dbReference type="OMA" id="MPSVMPW"/>
    <dbReference type="OrthoDB" id="26679at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF62">
        <property type="entry name" value="MUSTARD, ISOFORM V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="6">
        <location>
            <begin position="105"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="337" mass="38394" checksum="D610A7E975C390EB" modified="2011-11-16" version="1" fragment="single">MAFLLRKFSRDDSEKPSEEENLITLEDNDETKITNGKGDDKNVEPPDIEETSKSGSRIESFLRGLVRSPSRESSINSHSSCSSSSNLPPLSNLILSGYSASTRHKLLDLELANNIRNLIPARFQLFDSWELVYSMEQHGISLNTLYRHCNLDFQLRQFKKKKKAEKGFADSIVSNMVVGDISTSRYPIEGKRPIAYVLIIKDENNCKFGAYLNQNLKPMDQKRYYGNGECFLWKCERYIPHEFASDGTTKKGKEQVRFKAFMYTGINDNIIYSNHDFIAIGSSNGQNGIYIDKSLYKGVSYRCDTFGNEILNSAVYDDGRIGRFKIMGLEIWRIGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQH7</accession>
    <name>G3AQH7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_140190</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376303.1">
        <property type="nucleotide sequence ID" value="XM_007376241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQH7"/>
    <dbReference type="STRING" id="619300.G3AQH7"/>
    <dbReference type="GeneID" id="18870273"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140190"/>
    <dbReference type="eggNOG" id="ENOG502RQEU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1875168_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQH7"/>
    <dbReference type="OMA" id="GRNIRND"/>
    <dbReference type="OrthoDB" id="275783at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="135" mass="15687" checksum="FCD605133BAC74F3" modified="2011-11-16" version="1">MSWWNLFKIIGRNIRNDLVANIFERFPLLLIINPDSFQPPFTNGFDEYFNVTSPSIMNNQYSDVIVVNYMIVTLSILLAGLDIVYSIIRLSISTMIVGLFLGSMLFFSVWFLVTASRRWKVLVLMFQNNEFSGVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQR9</accession>
    <name>G3AQR9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCAAT-binding factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_155036</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376394.1">
        <property type="nucleotide sequence ID" value="XM_007376332.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQR9"/>
    <dbReference type="FunCoup" id="G3AQR9">
        <property type="interactions" value="1033"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQR9"/>
    <dbReference type="GeneID" id="18871007"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_155036"/>
    <dbReference type="eggNOG" id="KOG2038">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003417_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQR9"/>
    <dbReference type="OMA" id="EIWCNDE"/>
    <dbReference type="OrthoDB" id="28947at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030690">
        <property type="term" value="C:Noc1p-Noc2p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040155">
        <property type="entry name" value="CEBPZ/Mak21-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12048">
        <property type="entry name" value="CCAAT-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12048:SF0">
        <property type="entry name" value="CCAAT_ENHANCER-BINDING PROTEIN ZETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCAAT-binding factor" evidence="3">
        <location>
            <begin position="589"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="65"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="498"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="697"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="888"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="110"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="171"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="512"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="706"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="894"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="980"/>
            <end position="991"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1041" mass="118642" checksum="6AE642FAE3E36FAE" modified="2011-11-16" version="1">MDTSGLNLTSLKDKISSKLNSSKKPSKKTKKEGKKQDKKPDHEEEDILRREALALGATEEDLALLNDVDEGEESEQEFNDSGVKLDKSFGDDLSQFMKGIGLGSGGPIVVDDDEEEEEEDEDIPDLVDVPEEDEEDQEDEEAAEEKEEEQDDEESESSDESEEESESEDEVIVKESKIDNKKAKAQTSDKVTDVATVSSSKLVIPPRTDWYNVPFETTSQTPEKLDRFARERLLERAKATIDKDNKTYLEEFAANNSQRKFLSQILSDGTLNDKISALTLLIQEAPMHNIKAFDTLLSYCEKKSRTAALQSINALKDLLLNGVLPDRKLLPFEKQPLTKDASDVQLAVYYFEDYVKKAYFKLITILEVLSHDPIVHVRMNDVSHIFDLLKAKPEQEANLLKLGVNKLGDVDNKVASKTSYQILLLEQAHPAMKKIVTDAVIDVIFKNGGGDSHAKYYAITTLNQTILTRKDEELANSLVKTYFALFEKVLIENDANNKEKREDKTLGESSKGRKNNRKNFKKGKKGGKSVKVEEKSEQEIIEEKNTKLFSALLTGLNRAFPFSNLPREVFQNHLDTLFKITHSSNFNTSIQALVLVHHIITEQSLDSDRYYKTLYESLLDQRLANTSKQGIYLNLLFKSLKSDTNKARVLAFVKRILQICSHWLHVGAVSGMLYLLIQLVKTFPEISDLMIDVASRPDAEADEDKSEPKKDGQSAKDTEYDPKKRDPQYANADKSSLWEIGHFLNHYHPTIAIYASSLLEGTEQVKPDLGLYTLAHFLDRFVYKNSKQKPQTKGSSIMQPLGGLHTGSLLVRATNLMDTSVPANTEDWLNKKVEDVRPNEQFFHQYFTTKVNKIKTIKEDEDEKKKELLDDEEAAEMEDDEIWKALVKSKPDVEDNSEDDFSDFGEEDFSDLDMDEEEEDEDEEVNEEQQEQEEDDEEDDEEDDEEEGFEGEEQDIFGINEDDEFSDSEVELKMLGDAFGESEEEEEEEEEEKPKKKNKRAREDKDEGKKKSKKTKLKDLPVFASVDDYSQYLDSEDEDYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQX1</accession>
    <name>G3AQX1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_153999</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375978.1">
        <property type="nucleotide sequence ID" value="XM_007375916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQX1"/>
    <dbReference type="STRING" id="619300.G3AQX1"/>
    <dbReference type="GeneID" id="18870970"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_153999"/>
    <dbReference type="eggNOG" id="KOG0779">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_344519_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQX1"/>
    <dbReference type="OMA" id="HHWYCCI"/>
    <dbReference type="OrthoDB" id="442460at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019783">
        <property type="term" value="F:ubiquitin-like protein peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46915:SF2">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46915">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="351"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="283"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="647"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="648"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="709" mass="81785" checksum="EF9F631A5C96E070" modified="2011-11-16" version="1" fragment="single">MQSERPSLKEKNYSSISMRRKGSSITPGGVRPMNTLTIPKRSPSDNNHSEALEQHQHRSSASEKYTNSPPTKSTHESIVEKSKQIRERLAGLNDGHKYTKPQTLPPIQPSYSHEVKDCCDIGVNIKFNNLLEPPLVILQLKSFSFNGNRVKNNGKSNYVLSVSSNNKFLFITKDGKILDKYLIHRQTQLKSLAFSDDSVLIELQNQKGLIVATHYGQDQHLREYFNNHHEWNVDATKTIKDIELVLSRVKRAEQVHPVASTSPPPKSSIFDPLKNMKTTTPTRITRSNARQQMGGETEASPISIDQDDSVPSTTGGLWEDDIFQDDDFREHETPAPFVPDLKYQFPGNKVFTITATDFKTLYNNDWINDTVIDFFIQYEIDQALKANPKLDSNDIYAFNSFFFTKLTHKTTPQEAPDYYANIKRWLSKIQLMEYPYVIMPINEHAHWYGCIIRGLPDLLKVAKEEAEATTGGGHHPDQDHKPRKYVCEIFIFDSLSHKHANIKLPLKRFIIDYCKDIYNLNILKNQIRVVNARVPKQNNFNDCGIHVIYNIRKWINNIQECEKIWRTSHSSTQIQVSKTLFLAEERNGMRKQLINILLDLHAKQEQHTEIPKLQEDEDDVIFIEHRSTKPEEKPVEHQTVTTNYPKTLDPRVQEKPNRPNRPNFHNSELNVRFGERQVLNIGVTMLNELFPDQFAALPITIIENVQLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR50</accession>
    <name>G3AR50_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_61802</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376003.1">
        <property type="nucleotide sequence ID" value="XM_007375941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR50"/>
    <dbReference type="STRING" id="619300.G3AR50"/>
    <dbReference type="GeneID" id="18874056"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61802"/>
    <dbReference type="eggNOG" id="KOG0590">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_696701_0_0_1"/>
    <dbReference type="InParanoid" id="G3AR50"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF137">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE HRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="178"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="284"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="196"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="396" mass="45814" checksum="12249CC19C551FD0" modified="2011-11-16" version="1">MEYAPIDFFAVVMSGNMSRLEINCCLKQIIEGVNYLHNLGLAHRDLKLDNCVITTDGILKIIDFGSAVIFKYPYDQYGSKDAIHPCHGIVGSDPYLAPEVLKSPNSYNPQPVDLWSIAIIYCCMTLKRFPWKIPNSEKDNSFKLYCMEDDNWHDYYLSNECHKLLLQQRKLKNMIVRSNKKKKQVEQEQEQEEETPEPKPESEEPEKPTEESTEAMDIDKTPSIQTVQTDTEILSETQVQEILAQLKEIDDKLQQYEVTKNEMKQKFIDSRSKDPAYAAQLLAEEEERTKQAAKQAANPDEKKKPQHKQIHGPYRLMRLLPHASRPIIYKMLQVDPKKRATLEEIMQDDWIKEIQCCTLKRVARSADNIGANFDEDDDELLIKGTPEHEHTVVLDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARF1</accession>
    <name>G3ARF1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Maltose/galactoside acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_67782</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376536.1">
        <property type="nucleotide sequence ID" value="XM_007376474.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARF1"/>
    <dbReference type="FunCoup" id="G3ARF1">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARF1"/>
    <dbReference type="GeneID" id="18875183"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67782"/>
    <dbReference type="eggNOG" id="KOG4750">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051638_3_0_1"/>
    <dbReference type="InParanoid" id="G3ARF1"/>
    <dbReference type="OMA" id="WFGANSI"/>
    <dbReference type="OrthoDB" id="25818at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03357">
        <property type="entry name" value="LbH_MAT_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051159">
        <property type="entry name" value="Hexapeptide_acetyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024688">
        <property type="entry name" value="Mac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416:SF54">
        <property type="entry name" value="ACETYLTRANSFERASE, CYSE_LACA_LPXA_NODL FAMILY (AFU_ORTHOLOGUE AFUA_2G08430)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416">
        <property type="entry name" value="SIALIC ACID SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01266">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Maltose/galactoside acetyltransferase" evidence="3">
        <location>
            <begin position="43"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="257" mass="28764" checksum="DE5185CCC37B7A03" modified="2011-11-16" version="1">MPKFKSKEDFCKQTNIRGEINKELVEFAYKNLNHIPSKGNENYERMISGLIVLTFKHSYNAYTKDMQDVRMSIRDTILDYGKFRHRDYKTTKDYLDAKREHLRSFIGHVGDGAFMEYPVYFDYGFNTYIGKRFFSNYNLTILDVSIVKIGDNVMCGSNVSILTPSHPTDPTLRSGLQNGLPVTIGNNVWLGAGSTVLPGVTIGDGCVIGAGCVVNKDIPANSIVVGVPGRVVKTMDPRDPDFDVHACLEQYGMALIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARH4</accession>
    <name>G3ARH4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="17">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">SPAPADRAFT_72131</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="function">
        <text evidence="16">This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports the calcium to the vacuole and participates in the control of the cytosolic free calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 17">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="17"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376073.1">
        <property type="nucleotide sequence ID" value="XM_007376011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARH4"/>
    <dbReference type="FunCoup" id="G3ARH4">
        <property type="interactions" value="453"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARH4"/>
    <dbReference type="GeneID" id="18875417"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72131"/>
    <dbReference type="eggNOG" id="KOG0204">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002360_9_3_1"/>
    <dbReference type="InParanoid" id="G3ARH4"/>
    <dbReference type="OMA" id="YRMYVKG"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02081">
        <property type="entry name" value="P-type_ATPase_Ca_PMCA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000002">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000028">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000018">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 17" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 17" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 17" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="939"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="972"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1105"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1138"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="19">
        <location>
            <begin position="122"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="30"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="94"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EGW31295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1201" mass="131829" checksum="7BEFAD40E1014C91" modified="2011-11-16" version="1">MGSDKDWLNDSLPPITVNGNTPTIVEDADTTSPSSHQATGSTTLGGNSLQLPSRPSYEEASSTSSVTPLNKHLHDDDDFNIETTNIPAPTTRKAGGGGGGGGGGGSGSEELTTLHDPKSLRSLYEMGGFAKLCEMLKTSESGLDEKNETDLGGRRHYFGVNRLPERSSRGFFRLCWEAMKDKVLIILSIAAVVSLALGLYETFGAGTEYDDEGNPLPKVDWVEGVAILVAVCIVVIVGAANDYQKERQFAKLNAKKEDRELIVIRNGEQKMISIYDLLVGDVINLQTGDVVPADSILFDGDVECDESALTGESNTIKKVPVKQAMEIYQANLPTNEDIGSHVIQLRDPFLISGAKVMSGLGNALVTAVGEHSIHGRTMMSLSHEPETTPMQVKLDDLAEGISKYGFLAAIVLFVVLFIRFCVEIAPGGSYREHLPSEKGKMFIDIIITAVTIVVVAIPEGLPLAVTLALAFATTRMAQNGNLVRVLKSCETMGGATAICSDKTGTLTENKMRIVRGFFGLDQQQNLLEFNDTVVQSQQGTSLEVIDQIANDLKVFLCTNITLNSTAFENVEYDEEKARLAHERPHSQSLLSKLFSRNKQPEHYMELGIVDSPYLGNKTESALLILAKEKFHMFDDNSLDHIRSEAHSEVVQIIPFESSRKWSGIVLKIHNGYRIYVKGAAEIVFKNCGYQMNNAGQVIPINHTQRDTVLAKIDEYANDALRTIALGHCDFVGENIWPPNQVASSENPQEADANLLLDATANQISNKEYVLDALVGIQDPLKKGVPEAVLQCKRAGVVVRMVTGDNLNTAKAISKSCHILTPEDLSNEYAYMEGPTFRKLSLSQRNKIVPQLRVLARSSPEDKRILVDTLRKSGDVVAVTGDGTNDAPALKLADVGFSMGIAGTEVAREASDIILMTDDFTDIVQAIKWGRTVSISIKKFIQFQLTVNITACVLTFVSAVASSENKSVLTAVQLLWVNLIMDTLAALALATDKPDDSFLNRKPAGRTAPLISTSMWKMILGQSTTQLVITFVLHFAGKILFFGKNANINNHQSQQLNAMTFNAFVWLQIWKLFVTRKLDEADEITTVRGRITMENLNFFSHLFRNWYFIIIALLISGCQILIMFVGGASFSIAKQTPGMWATAILCGFISIPMGLIIRIIPNVWVERIFPTRAFNKLLYILSFGWLRRKKRSDIELEEGDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARL2</accession>
    <name>G3ARL2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62360</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376543.1">
        <property type="nucleotide sequence ID" value="XM_007376481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARL2"/>
    <dbReference type="FunCoup" id="G3ARL2">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARL2"/>
    <dbReference type="GeneID" id="18874287"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62360"/>
    <dbReference type="eggNOG" id="ENOG502SAHB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061844_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARL2"/>
    <dbReference type="OMA" id="DFIWYSI"/>
    <dbReference type="OrthoDB" id="2567806at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031349">
        <property type="entry name" value="Tfb6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37781:SF1">
        <property type="entry name" value="ADR380WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37781">
        <property type="entry name" value="TFIIH COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17110">
        <property type="entry name" value="TFB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="342" mass="39370" checksum="9FD798C664BA1187" modified="2011-11-16" version="1">METSSTPPTPVHPGPNEEVRTLPDETIPQSTLADIDLNPDLYDDSQTPTPSVDISKPVPSSSNAVGPSPSAHPHHHEVTPTQQLHQHQHQNSHIPHVSSTTSMDDSETDMEEVDLFKFQQITLPFDEAYQLFKLDFESIEQHEPISHQQQSKFVNYIDEQLLQIQRKFIKSQAETNETYSFGQLIQELTDVINIIWVSVCQKNTLFGQMDYYIKILGDLEDYLEHYRNIFDSDLTQYNVKIDVSKMVAFFKFFQKLDLQLSLLMDGFTANTNQTTKASNTELIRLYPIISRLRILIISRVENLRTKLNKNVTSTNRQDSQNLLNLFELEISRLFEGILERSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3ART3</accession>
    <name>G3ART3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NADH:ubiquinone oxidoreductase 30kDa subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_56212</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the complex I 30 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376128.1">
        <property type="nucleotide sequence ID" value="XM_007376066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ART3"/>
    <dbReference type="STRING" id="619300.G3ART3"/>
    <dbReference type="GeneID" id="18872016"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56212"/>
    <dbReference type="eggNOG" id="KOG1713">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042628_0_0_1"/>
    <dbReference type="InParanoid" id="G3ART3"/>
    <dbReference type="OMA" id="PCRKNRF"/>
    <dbReference type="OrthoDB" id="37721at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.80:FF:000002">
        <property type="entry name" value="NADH dehydrogenase iron-sulfur protein 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.80">
        <property type="entry name" value="NADH:ubiquinone oxidoreductase, 30kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01357">
        <property type="entry name" value="NDH1_NuoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010218">
        <property type="entry name" value="NADH_DH_suC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037232">
        <property type="entry name" value="NADH_quin_OxRdtase_su_C/D-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001268">
        <property type="entry name" value="NADH_UbQ_OxRdtase_30kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020396">
        <property type="entry name" value="NADH_UbQ_OxRdtase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01961">
        <property type="entry name" value="NuoC_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004733">
        <property type="entry name" value="PRK06074.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10884:SF14">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10884">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00329">
        <property type="entry name" value="Complex1_30kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143243">
        <property type="entry name" value="Nqo5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00542">
        <property type="entry name" value="COMPLEX1_30K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 10" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase 30kDa subunit" evidence="12">
        <location>
            <begin position="102"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="287" mass="33151" checksum="9092EDD6D5197D8C" modified="2011-11-16" version="1">MISSKSTQVLRKALQQTPRLITASATRSFATTAIRHNTTDDLPFLNNLPRKKPVGENYVPLVNPTEKYKEQIEELHKFGTYLITCLPKFVQQFSVWKDELTIYVAPSALRPVMIFLKNHTAAQFKSCMDVTAADYPSRTNRFDVVYNLLSVRHNSRIRVKTYASETSPVPSVVPIFQGANWYERETYDLFGVFFEGHPDLRRIMTDYGFEGHPLRKDFPTTGYTEIRYDEEKKRIIYEPLELTQAWRNFTVGASVWEPVGEGKDFTPEAFKLPTPQPEPEKEGEQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARY8</accession>
    <name>G3ARY8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Phosphoglucomutase/phosphomannomutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_62449</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376615.1">
        <property type="nucleotide sequence ID" value="XM_007376553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARY8"/>
    <dbReference type="FunCoup" id="G3ARY8">
        <property type="interactions" value="318"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARY8"/>
    <dbReference type="GeneID" id="18874322"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62449"/>
    <dbReference type="eggNOG" id="KOG1220">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_0_1_1"/>
    <dbReference type="InParanoid" id="G3ARY8"/>
    <dbReference type="OMA" id="GYCVDPE"/>
    <dbReference type="OrthoDB" id="8300170at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008973">
        <property type="term" value="F:phosphopentomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="PGM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000035">
        <property type="entry name" value="Pgm3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
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        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745:SF1">
        <property type="entry name" value="PHOSPHOGLUCOMUTASE 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745">
        <property type="entry name" value="PHOSPHOMANNOMUTASE 45A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="51"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="151"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="12">
        <location>
            <begin position="244"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="13">
        <location>
            <begin position="358"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW31837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="623" mass="70590" checksum="586DA9DBCFFC0C21" modified="2011-11-16" version="1">MNITNEHKQQLDTLVESWISIDINPQSKQEILELQSQQNYDVLSKKLSHRIAFGTAGLRSSMESGFAHMNDVTVLQASQGLIEYLISVYKEETSIVVGYDHRYNSQRFAEILTSVAVSKGVKVYYLGSVENLSKQTLELSETSYDNNDLASVGYVHTPLVPFAIDFLKAKGGVMITASHNPARDNGYKVYFSNGCQIIPPQDQHIAESIERNLQPWEGVWDVAGKILSGKKQNLIEPVRELITKEYLEGIKSKLITNDKLSFNFVYTPMHGVGLDIFQQCIDLFAHRGKYEVVKQQAKPDPEFPTVKFPNPEEKGALDLSISHAKNLGYKLVVANDPDADRFTVAIETKTGVWKQLTGNEIGFLFAVYFIEEVVPKESLKDVYLVNSTVSSQILDAMAENQGFHFAETLTGFKWIGNKAIDLEKSGYKVPFGYEEAIGFMFNLVHDKDGVSAAVIWLQLYEKWFADGKLDPVEKLEQGYSKYGWFKEYNGYYRFQDGALLNQIFELIRKSYTGHYPETIGSFKVVSWRDLTIGYDSTSKDNIPLLPIDPTSQMITAVLKRGSGDESVRFTCRGSGTEPKLKLYIEGKSKTSETIAREIAKECWETLREEWFKPEKYELEEVHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASD6</accession>
    <name>G3ASD6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_155767</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Lipid-anchor</topology>
            <topology evidence="10">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376708.1">
        <property type="nucleotide sequence ID" value="XM_007376646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASD6"/>
    <dbReference type="FunCoup" id="G3ASD6">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASD6"/>
    <dbReference type="GeneID" id="18871028"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_155767"/>
    <dbReference type="eggNOG" id="ENOG502QRZZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021855_1_2_1"/>
    <dbReference type="InParanoid" id="G3ASD6"/>
    <dbReference type="OMA" id="WTQEAND"/>
    <dbReference type="OrthoDB" id="421038at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000032">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF14">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 10" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9 10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005131582" description="1,3-beta-glucanosyltransferase" evidence="10">
        <location>
            <begin position="26"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="440"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="444"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="466"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW30675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="512" mass="58015" checksum="6ECF0F64F91B96CB" modified="2011-11-16" version="1">MHYINLISFKVLIVLSILFINQVGAYVHPITIHGHFFVDSVTKEPFYIKGIDYQPGGSSAVSNHKDPLSDPEICARDIILFQELGINTIRIYSVNADLDHNVCMTMLATAGIYLVLDVNSPLPNHHLNRYEPWTTYNLFYLENVFKIVEQFSYYNNTLGFIAGNEVVSDPTSASVAPPYIKGMIRDIKTYIEYNSPRTIPVGYSAADDLNYRMSLASYLECADENPAESVDFYGVNSYQWCGEQTFYSSGYNILVNDYHGFTRPVFFSEYGCNEVLPRNFDEVQVLYTNDMIDVFSGGLVYEFTQEPNNYGLVEVQPNGDIKLLRDFMQLKKQFDSIPELDYAFVANSMRTNAREIHSKLKQYKSAIPKCGESYPNIGISIGIPVSIAENLIETGVKVKPGHYVHLEPHQLTTKYNIFQANGEKYPTYLSIDILVNVTQDEPESSVTPPSVVPHVKNPHSPPPETTEIEEEFPNEEEDVAESDPDNTTTKSFRGMFQRVATSFKKLYSSLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASN8</accession>
    <name>G3ASN8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N(6)-L-threonylcarbamoyladenine synthase</fullName>
            <ecNumber evidence="1">2.3.1.234</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">QRI7</name>
        <name evidence="9" type="ORF">SPAPADRAFT_62582</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in mitochondrial tRNAs that read codons beginning with adenine. Probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Involved in mitochondrial genome maintenance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-threonylcarbamoyladenylate + adenosine(37) in tRNA = N(6)-L-threonylcarbamoyladenosine(37) in tRNA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="7">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="1"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376757.1">
        <property type="nucleotide sequence ID" value="XM_007376695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASN8"/>
    <dbReference type="FunCoup" id="G3ASN8">
        <property type="interactions" value="422"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASN8"/>
    <dbReference type="GeneID" id="18874387"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62582"/>
    <dbReference type="eggNOG" id="KOG2707">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023208_4_1_1"/>
    <dbReference type="InParanoid" id="G3ASN8"/>
    <dbReference type="OMA" id="NAAMIGC"/>
    <dbReference type="OrthoDB" id="10259622at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:tRNA N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072670">
        <property type="term" value="P:mitochondrial tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01445">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017860">
        <property type="entry name" value="Peptidase_M22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022450">
        <property type="entry name" value="TsaD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF6">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01016">
        <property type="entry name" value="GLYCOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Gcp-like" evidence="8">
        <location>
            <begin position="60"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW30724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="431" mass="48296" checksum="C27A3525B5770AB9" modified="2011-11-16" version="1">MMICRNIIRKSPRSLQHIRSYKVLAIESSCDDSCVALLDKYHPDKPPLVIDQFKKTLDSADVGGIVPTAAINFHHTNVASLAKDLCAKHNISSTNPPDLICVTRGPGMVGSLSSSIQFAKGLSVAWDVPLVGVHHMLGHLLTAQLPKTEQPDIGPPEYPFLSLLCSGGHTMVILSKSLTDHEIILDVSGIAVGDSLDKCARELGMHGNMLGKELEKFVDAIPQEEIDEFERMDMSTRKANDYNFKLTIPFSGAFSKDDMYFQFAQFLSSVQQYKAYYKGKVDEGEQYDPKTKRMLAYMIQERIFDHMINRINMAFKIHGTGPRADGKLVGVKHFICSGGVASNKRLRKKLETELKLDHLDVPSMTFHFPDLSLCTDNAIMIGNAGINLFEQLKLKSSLEIMPIIRWPMDKILDVDGWIEMSDDEYNNVRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASW7</accession>
    <name>G3ASW7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mitochondrial distribution and morphology</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_62604</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRIAP1/MDM35 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376782.1">
        <property type="nucleotide sequence ID" value="XM_007376720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASW7"/>
    <dbReference type="FunCoup" id="G3ASW7">
        <property type="interactions" value="191"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASW7"/>
    <dbReference type="GeneID" id="18874399"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62604"/>
    <dbReference type="eggNOG" id="KOG3481">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101473_2_0_1"/>
    <dbReference type="InParanoid" id="G3ASW7"/>
    <dbReference type="OMA" id="KKYDDCF"/>
    <dbReference type="OrthoDB" id="19091at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990050">
        <property type="term" value="F:phosphatidic acid transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120010">
        <property type="term" value="P:intermembrane phospholipid transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033108">
        <property type="term" value="P:mitochondrial respiratory chain complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007918">
        <property type="entry name" value="MDM35_apoptosis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46403">
        <property type="entry name" value="TP53-REGULATED INHIBITOR OF APOPTOSIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46403:SF1">
        <property type="entry name" value="TP53-REGULATED INHIBITOR OF APOPTOSIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05254">
        <property type="entry name" value="UPF0203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="88" mass="10093" checksum="C4B9A2E2B4D38084" modified="2011-11-16" version="1">MSVSFAPECTDAKTKYDDCFNKWYTEKFLKGQSLNNECSEFWDTYITCINANLAKKGIKPMLDKAREDQPFTSEEMKESAGTASKEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASX2</accession>
    <name>G3ASX2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ceramide glucosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.80</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Glucosylceramide synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">UDP-glucose ceramide glucosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">UDP-glucose:N-acylsphingosine D-glucosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_62607</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.80" evidence="5"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376787.1">
        <property type="nucleotide sequence ID" value="XM_007376725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASX2"/>
    <dbReference type="STRING" id="619300.G3ASX2"/>
    <dbReference type="GeneID" id="18874401"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62607"/>
    <dbReference type="eggNOG" id="KOG2547">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030898_1_0_1"/>
    <dbReference type="InParanoid" id="G3ASX2"/>
    <dbReference type="OMA" id="IVWIIDC"/>
    <dbReference type="OrthoDB" id="1483400at2759"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008120">
        <property type="term" value="F:ceramide glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006679">
        <property type="term" value="P:glucosylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025993">
        <property type="entry name" value="Ceramide_glucosylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12726">
        <property type="entry name" value="CERAMIDE GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12726:SF0">
        <property type="entry name" value="CERAMIDE GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13506">
        <property type="entry name" value="Glyco_transf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW30754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="543" mass="62660" checksum="93A452F6F0AB7A3B" modified="2011-11-16" version="1">MDSYARLISSYFFLAWYLFIVLVAYIGFVEILLKFRKRNILWKVNHEKQDQNEDQYEGVTIIRPIKGIDPELSSCLESSFCQSYPISKLQILFCVDNPSDPAIPIIRSLIKKYSHIDSQILVSENFNEVTKTSEDHYGPNPKVNNLAKAFVSAKYDILWIMDSNVWASSNILKNSVITLNQNLNNGRQLNSQRKVKLVHHVPLAMSISKKTYEEEDDIDLEYGLTPQQSHDSDSSTSNSSSANVHIGVAKRKSPLRQQIRVADSFEQEESPRKVRFLDKLGAKLDEMFLHTSHSKFYVALNNVAVAPCVNGKSNMYRRSELDRAVSLIASHDSPFFHDPIVKQQAVYYSSLGPGHAIKFFARYIGEDNMIGIALWENCFARTGLTGDVVVQPIFESNNTINDYIQRRVRWLRVRKYMVLLATLIEPTTESLICGVYGTYAVSSIFFNCWFHWGWFLAHMIVWMMTDYIQYHTFTNHVTDASEVNYMPTWLRYKPAGSRLDWLRVWAVREVLALPIWIIAMVGHEIDWRGKPFMIKKDLTAEEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASY4</accession>
    <name>G3ASY4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CUE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_62617</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376799.1">
        <property type="nucleotide sequence ID" value="XM_007376737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASY4"/>
    <dbReference type="GeneID" id="18874408"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62617"/>
    <dbReference type="eggNOG" id="ENOG502S359">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1574513_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASY4"/>
    <dbReference type="OMA" id="GCGANPL"/>
    <dbReference type="OrthoDB" id="5396806at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="CUE" evidence="3">
        <location>
            <begin position="18"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="60"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="120"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="170" mass="18903" checksum="1A970792FAA7A986" modified="2011-11-16" version="1" fragment="single">MSTQQRKIYKNQSKRFNTPSIELTNLLEMFPDWESEDLQSLLQEHDNSLEVVIDLIVNNKVSKWEPSKKEKQHKKKEHKDDQESPVNNASPANNTASSKQSKSKGPYAGPKPRRERAPKKTTQQQQQSQPTSAGSVSSSTTATAPVASTSPVSNSWASALSSDKPKQEIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATB1</accession>
    <name>G3ATB1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Multidrug resistance protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_142204</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376907.1">
        <property type="nucleotide sequence ID" value="XM_007376845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATB1"/>
    <dbReference type="FunCoup" id="G3ATB1">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATB1"/>
    <dbReference type="GeneID" id="18870430"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142204"/>
    <dbReference type="eggNOG" id="KOG0255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008455_11_1_1"/>
    <dbReference type="InParanoid" id="G3ATB1"/>
    <dbReference type="OMA" id="SRESTHW"/>
    <dbReference type="OrthoDB" id="3357846at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015244">
        <property type="term" value="F:fluconazole transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00880">
        <property type="entry name" value="2_A_01_02"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF23">
        <property type="entry name" value="FLUCONAZOLE RESISTANCE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="188"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="364"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="410"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="439"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="512"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="99"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="545" mass="60435" checksum="4AF6CB281EAD6705" modified="2011-11-16" version="1">MVPTLIRDSFVGRLAYQLSGHKYFSYPEEHPDYIVPEKYLSEGKVSDFDKVEIDEGVPSNSSIDSEQAKPQDENIIVTWDGDDDPDNPNNWSFSKKAFVIFQVSFLTTSVYMASAVYTPGIEQIMEEFQIGEVVATLPLTLFVLGYAIGPLIFSPLSENAAFGRNAIYIYTLAIFVILQIPTALVHNIAGLCILRFITGVFASPCLATGAATVADIVPIPYIPVGLAAWSVGAVCGPSFGPFFGSILTVKGGWRWTFWFICIVAGFSLVFLSFTFPETYAKTLLYRRAIRLRGVTGNQRITSEGELENRNKTARQIAIETLWRPIEVFIMEPVILMINLYIALVYSTWYLFFEVYPIYFQGVRGFTIVELGCTYLATIIGVVITASIYIPTVRQVFTKRIFSGKGVQPEVFMPIAIVGAICMPVGLFIFGWSATTHTHWIGPLIGGAIFSAGGFLIFQTMFNFIAGSFIYVGSIFASNNLFRSLSAGCFPLFGRPLYNNLATKEFPVAWGSSLLAFITVAMIAIPVLFYLNGPKLRARSKYALHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATE1</accession>
    <name>G3ATE1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome b mRNA-processing protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_68133</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Essential for the assembly of ubiquinol-cytochrome c reductase. It has a direct effect on the correct occurrence of the Rieske protein, core 4, core 5 and apocytochrome b.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
            <topology evidence="1 10">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the CBP4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376937.1">
        <property type="nucleotide sequence ID" value="XM_007376875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATE1"/>
    <dbReference type="FunCoup" id="G3ATE1">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATE1"/>
    <dbReference type="GeneID" id="18875198"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68133"/>
    <dbReference type="eggNOG" id="ENOG502S2G8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147520_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATE1"/>
    <dbReference type="OMA" id="YRWARVG"/>
    <dbReference type="OrthoDB" id="5576752at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012420">
        <property type="entry name" value="Cbp4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28202">
        <property type="entry name" value="ASSEMBLY FACTOR CBP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28202:SF1">
        <property type="entry name" value="ASSEMBLY FACTOR CBP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07960">
        <property type="entry name" value="CBP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW30904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="146" mass="17296" checksum="F120A1308BE868AC" modified="2011-11-16" version="1">MSTKPLWYRWARVYFAGGCIIGTGVLLYNTIRPSEEDLIASFSPEVRRDYERNKALRELEQQRLMEIVKQTAKSDEPIWKTGPIGSPFEKDQRNLGQQLVDYEGVRRDQALDESKEEVERAKAEMREAEALISGGEKKKWWWWWWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATP1</accession>
    <name>G3ATP1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate dehydrogenase assembly factor 3</fullName>
            <shortName evidence="6">SDH assembly factor 3</shortName>
            <shortName evidence="6">SDHAF3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_143199</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Promotes maturation of the iron-sulfur protein subunit of the SDH catalytic dimer, protecting it from the deleterious effects of oxidants. May act together with SDHAF1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with the iron-sulfur protein subunit within the SDH catalytic dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the complex I LYR family. SDHAF3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377238.1">
        <property type="nucleotide sequence ID" value="XM_007377176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATP1"/>
    <dbReference type="FunCoup" id="G3ATP1">
        <property type="interactions" value="276"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATP1"/>
    <dbReference type="GeneID" id="18870525"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143199"/>
    <dbReference type="eggNOG" id="KOG4100">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102310_1_0_1"/>
    <dbReference type="InParanoid" id="G3ATP1"/>
    <dbReference type="OMA" id="WQQTNEN"/>
    <dbReference type="OrthoDB" id="278329at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015976">
        <property type="term" value="P:carbon utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006111">
        <property type="term" value="P:regulation of gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006105">
        <property type="term" value="P:succinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20270">
        <property type="entry name" value="Complex1_LYR_SDHAF3_LYRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008381">
        <property type="entry name" value="SDHAF3/Sdh7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137">
        <property type="entry name" value="DC11 ACN9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137:SF6">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13233">
        <property type="entry name" value="Complex1_LYR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="125" mass="14833" checksum="3ACABA9A1AE24FD9" modified="2011-11-16" version="1">MRSTAVRLVRQRRPTRNESPLLPPLVLYRSILRAHVHKLPKDLRSLGDHYVKDEFKAHKKIDNPLHIVAFLTQWQDYLRQIDHGSWLHNKLKHEDLEKMSPDQIGQLYELMKAAKRVDEDGEHTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATR8</accession>
    <name>G3ATR8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Crossover junction endonuclease MUS81</fullName>
            <ecNumber evidence="16">3.1.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_157116</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15 16">Interacts with EME1 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication fork intermediates. May be required in meiosis for the repair of meiosis-specific double strand breaks subsequent to single-end invasion (SEI).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with EME1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the XPF family.</text>
    </comment>
    <dbReference type="EC" id="3.1.22.-" evidence="16"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377265.1">
        <property type="nucleotide sequence ID" value="XM_007377203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATR8"/>
    <dbReference type="FunCoup" id="G3ATR8">
        <property type="interactions" value="194"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATR8"/>
    <dbReference type="GeneID" id="18871067"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157116"/>
    <dbReference type="eggNOG" id="KOG2379">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014329_1_0_1"/>
    <dbReference type="InParanoid" id="G3ATR8"/>
    <dbReference type="OMA" id="ELGDAMW"/>
    <dbReference type="OrthoDB" id="5963188at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048476">
        <property type="term" value="C:Holliday junction resolvase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048257">
        <property type="term" value="F:3'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd21036">
        <property type="entry name" value="WH_MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20074">
        <property type="entry name" value="XPF_nuclease_Mus81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000307">
        <property type="entry name" value="Crossover junction endonuclease MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000005">
        <property type="entry name" value="crossover junction endonuclease MUS81 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.670">
        <property type="entry name" value="Crossover junction endonuclease EME1, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.110">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027421">
        <property type="entry name" value="DNA_pol_lamdba_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042530">
        <property type="entry name" value="EME1/EME2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010996">
        <property type="entry name" value="HHH_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033309">
        <property type="entry name" value="Mus81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047417">
        <property type="entry name" value="WHD_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047416">
        <property type="entry name" value="XPF_nuclease_Mus81"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451">
        <property type="entry name" value="CLASS II CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451:SF0">
        <property type="entry name" value="CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14716">
        <property type="entry name" value="HHH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21136">
        <property type="entry name" value="WHD_MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47802">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 16" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="12 16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 16" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0469">Meiosis</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 16" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="18">
        <location>
            <begin position="258"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00058015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU369042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW30294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="535" mass="61577" checksum="F09C3DBF75B1F448" modified="2011-11-16" version="1">MDDYKHLFIQWLEEEAIAATKKGSKLALLYNRALDQVRRYPSEITTLKTLRSIKFVGEKTAISLKRKLISHCQTEGIELPESFDPAPEETGHPTKKQRVTKPRKYIPKYRSGGYAILIALYHHDKANRGLSKDSVIEYAAKHCDKSFTSNAAASDFYSAWTSVKTLITHDLVSCTGRSPKLYFLTEEGKELAQKLRSTQDISSPVASSQSEMSFDNGVRYMSSPIPRKPATVYDHDVNNRIYNGTKYEIWHKGEYEIILCLDNREIRSKQDRDFFPTRLAQLDTECQVRALSVGDAVWIAKHKQTNKEVVLNYICERKRLDDLASSLRDGRFHEQKSRLKKTGMKHYYYLVEDISGVFNEINGESLQTAVSATMTTSRLYLRRFRDIDETTAFLASTTKVIANITSTLLVLRPADLKNQQEYTTLLQFFREKFEPKYECVHQFQTFQDMLGKTNVMTVREMFILMLMTIRGVSLERAIVIQKRFSTPKQLIEFYREGGDKDSLMKEMDSLGLVGNKKIGKAVSERVFEVWGCKHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATV9</accession>
    <name>G3ATV9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ERCC4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_143000</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EME1/MMS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377307.1">
        <property type="nucleotide sequence ID" value="XM_007377245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATV9"/>
    <dbReference type="STRING" id="619300.G3ATV9"/>
    <dbReference type="GeneID" id="18870509"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143000"/>
    <dbReference type="eggNOG" id="ENOG502RY0Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076918_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATV9"/>
    <dbReference type="OMA" id="RITSEHY"/>
    <dbReference type="OrthoDB" id="343092at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048476">
        <property type="term" value="C:Holliday junction resolvase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033310">
        <property type="entry name" value="Mms4/EME1/EME2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21077:SF5">
        <property type="entry name" value="CROSSOVER JUNCTION ENDONUCLEASE MMS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21077">
        <property type="entry name" value="EME1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0469">Meiosis</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="15">
        <location>
            <begin position="55"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW30336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="337" mass="38699" checksum="710D15AD92AB46E5" modified="2011-11-16" version="1" fragment="single">RPQKKSKKAEPSLDTPQVDGFKYSHKELVNANKVTKKKEELFAEMELGIESSVYELFTDTSDNFQCRITSEHYSQPIISWNRHINSRYDLIRDIFIPCPPTTITEPVVVLYYTADTFIARLNDKTLTGDVISSKIALDNVRGDETPHVILVVEAYDHLLNKIKAYEQRQFKNQVLERMGSPTKQTKRKTPNDIESYPPSKEIEQLVNQIQIELSINIFMVRTRLEAINWLNSFTYTIASSLYDKYERNKDLANIGTVRSGSDAKSTFLQSIQQFNLMTESKADRLYVQYKTMNQVYTTLKAQGTLGQDNFGRNIVPPSVDSALHKFFTADDPDDIIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU30</accession>
    <name>G3AU30_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">NADP+-dependent malic enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MAE1</name>
        <name evidence="10" type="ORF">SPAPADRAFT_63256</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="7">Divalent metal cations. Prefers magnesium or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the malic enzymes family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377377.1">
        <property type="nucleotide sequence ID" value="XM_007377315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU30"/>
    <dbReference type="FunCoup" id="G3AU30">
        <property type="interactions" value="900"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU30"/>
    <dbReference type="GeneID" id="18874660"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63256"/>
    <dbReference type="eggNOG" id="KOG1257">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011405_5_2_1"/>
    <dbReference type="InParanoid" id="G3AU30"/>
    <dbReference type="OMA" id="QIVNHMV"/>
    <dbReference type="OrthoDB" id="5365701at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004471">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="NAD-dependent malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
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    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010052">
        <property type="entry name" value="PRK13529.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406">
        <property type="entry name" value="MALIC ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406:SF34">
        <property type="entry name" value="NAD-DEPENDENT MALIC ENZYME, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="9">
        <location>
            <begin position="124"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="8">
        <location>
            <begin position="315"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
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        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="498"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="548" mass="60437" checksum="738DAAD7383CA7CA" modified="2011-11-16" version="1" fragment="single">MLKFKVQIRLASTAASVGKHTSVHTHTIKTPVDIRAAIATLKPKTTRLSIEGPIECDLQGFSLLNSPIFNKGSAFTQEERKAFGLEGLLPRQINTLDEQVERAYRQFCFLKTPLAKNDFLTSMRIQNKVLYYELVRNHIREMLPIIYTPTEGDAIAAYSHRFRKPEGCFLDINDPDSIDTRLSAYGEDKDIDYIVVSDGEGILGIGDQGVGGIRIAIAKLGLMTLCGGIHPGRVLPIVLDVGTNNKKLLNDDLYMGNKMDRVRGDQYWDFIDKFIQAVKKRFPSAVLHYEDFGVSTGRPMLYKYREELPSFNDDIQGTGAVVMASMTAALQFSNRDLLDAQIVIYGAGSAGLGIADQIVNHMVSHGSTVEEARSRIHCMDRYGLILDNSTTGSEAQMRYADPASEWEGVNTASLVDVIAKVKPTTLVGCSTQAGAFDETVIKEMYKHNKQPIVFPLSNPTRLHEAVPVDIMRWTDNNALIATGSPFEPVDGYYISENNNCFTFPGIGLGAVLSRCTTISDTMVSAAVDKLASLSPKMKNPKNGLLPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUB3</accession>
    <name>G3AUB3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_63310</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="AlphaFoldDB" id="G3AUB3"/>
    <dbReference type="GeneID" id="18874701"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63310"/>
    <dbReference type="HOGENOM" id="CLU_2062922_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUB3"/>
    <dbReference type="OrthoDB" id="4092372at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="119" mass="13331" checksum="B7AB7391D4E95F6D" modified="2011-11-16" version="1">MIYSKRSINTEPFSCQLDMIEKTKIEIKNQPPLYNKRFSKRTSYRPQQPLTTAPVVDQKVFAPIITTTSISPANKTPAMFDPFKPVDFSIGLTKDIWGTTTPTSAARTTTTTGRSTVWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUZ6</accession>
    <name>G3AUZ6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_157951</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPS family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377618.1">
        <property type="nucleotide sequence ID" value="XM_007377556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUZ6"/>
    <dbReference type="FunCoup" id="G3AUZ6">
        <property type="interactions" value="1047"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUZ6"/>
    <dbReference type="GeneID" id="18871107"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157951"/>
    <dbReference type="eggNOG" id="KOG2073">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003676_2_0_1"/>
    <dbReference type="InParanoid" id="G3AUZ6"/>
    <dbReference type="OMA" id="HAYIACE"/>
    <dbReference type="OrthoDB" id="295029at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007587">
        <property type="entry name" value="SAPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634:SF8">
        <property type="entry name" value="FIERY MOUNTAIN, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634">
        <property type="entry name" value="SIT4 YEAST -ASSOCIATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04499">
        <property type="entry name" value="SAPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="91"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="677"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="725"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="151"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="680"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="727"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="740"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="757"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="826"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="836"/>
            <end position="845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW29852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="845" mass="94889" checksum="37356DF58B7E3912" modified="2011-11-16" version="1">MSGSFWKFTNGFTSLSNISTLLENHATSQDEKPNVSESYTVLLKLLDENDLLQELLSNNPMLLEFLRNDVIVGMLVDLVINEGVIDAPEDTDLISKETADTTTSEASTGKEESEKESEKESDKESEKSDEAETETDAETDKESESEKSEESDSESESEDLNEETPEDRANRRATIASEILSADVWSLTDTVMESTANLNSLWSILDTKEPLSINVSTYFMKIMEHLLDMKCDDMISYLIENQPNLVEKFINHLSNPPLMDFLLKLISTDKPDNATGIIEYLQSQNLITHLINSLDIDPNIDPEQVIINQSSAGDFLKALITISANSTTDNSTIGPNELTRELVSPEQMQRLCDIMLKGGYALANGVGIIIEIIRKNNSDYDILPILYISLESHPPTGRDPIYLGHLLRVFGEQIPQFNKLLIKDYSNLKLKTPFGEIEPLGFERFKICELIAELLHCSNMALLNDNRGFDIVSRRDAVRNKMKEYDPISFKYNEIIVLPDMDPEPEAPVEEDEEAPHANANLSEAELRADPVVGDLVKISVFDTQIISNILSMFFRFPWNNFLHNVVFDIVQQVLNGSMDIGFNKFLAIDLFVNGDIINKITEGQKLCQDYELTHNGLRLGFMGHLTLIAEEVVKFIQLYPGDSLSVLINEKIDNEVWESYVNNVLYDTREKYNAILGGGDEESEPEDEPEGDTSIDEGLNEIIEEVKTGLGFDGEPQLKKKHLLETEESESDDDDHFSNYMSQQLTSPAAQPDEFTSSEEEEEIVPDDDDDDYIDPNDDGLSYKKPNPLYDASGSLKSGPVTLREQVGFNDEDEDEFSSSSSSSSDEEEEEEQETQLTRAASQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV36</accession>
    <name>G3AV36_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycoside hydrolase family 125 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_57566</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377876.1">
        <property type="nucleotide sequence ID" value="XM_007377814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV36"/>
    <dbReference type="STRING" id="619300.G3AV36"/>
    <dbReference type="GeneID" id="18872128"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57566"/>
    <dbReference type="eggNOG" id="ENOG502QR7D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023537_1_1_1"/>
    <dbReference type="InParanoid" id="G3AV36"/>
    <dbReference type="OMA" id="WFAWCNS"/>
    <dbReference type="OrthoDB" id="7771656at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008313">
        <property type="entry name" value="GH125"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31047">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 157 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31047:SF0">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 157 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06824">
        <property type="entry name" value="Glyco_hydro_125"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028846">
        <property type="entry name" value="UCP028846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01149">
        <property type="entry name" value="DUF1237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="511" mass="57784" checksum="5477039FBD166710" modified="2011-11-16" version="1">MDKHTSKCPDYVEYSKHPHPPYSSPEYQYPYMRPAPKCRTFVSSAVEKLVQDLVSKVKNPDLARLIENCLPNTLDTTILWHAEVEDHRPQTFVVTGDIHAEWLRDAARQLSVYQPLIKHDEKLQTLILGAINTQAYYVNNSPYCNAFHPPPHSGVKKGSTAMDDVHPRPDWKQVFECKYEIDSLASFLTLSNEYFEHADTKVKPAFITPEWLRALEKLLIVLRREAQPSFDEDTGQALPYYYSFQRHTNIGSETLALGGVGNPVNFGTGLIRSAFRPSDDATVLQFFIPGNAHMVTELIRARKMYLNADMIDGIQGLTSAVDEFIAKIGKGIQEHGIVQHPKYGKVYAYEVDGYGGSLFMDDANIPSLLALPDMGFTSVDDPVYQNTRKMILAKEGNPYYLKGAFFEGIGGPHVGIRSAWPMSLLVRMRTTDDDEEILRSLKLVMETTAGLGLMHETVDVNSRGGHQYSRSWFAWCNSEFGKTILHLAKHKPHLIFKDEFSQSYDIDKVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVE2</accession>
    <name>G3AVE2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_63768</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377927.1">
        <property type="nucleotide sequence ID" value="XM_007377865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVE2"/>
    <dbReference type="GeneID" id="18874906"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63768"/>
    <dbReference type="eggNOG" id="KOG0253">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_52_2_1"/>
    <dbReference type="InParanoid" id="G3AVE2"/>
    <dbReference type="OMA" id="TWRNYTL"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000171">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF4">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="348"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="421"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="446"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="480"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="508"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="78"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="537" mass="59784" checksum="857C64A34AE89C49" modified="2011-11-16" version="1">MNSREKQPITLDKNAETELLPPPSNDLTKEDYLVNSEDRSSSEIDLDMDDLDGNDHILHEKMKLINDAIDEIGFTPYHLKLFFLNGMGYWTDTQLMYLESSVRTFINYQFDYDFPVTAMTYGVGMILGGLFWGFGADLIGRRLAFNLSLMLSAIFTIMTGIMNSMASYALFILLIAFAIGGNLVLDTCVFLEYLPHKDQWLLTFFAFFWGIGQVIAVGLAYAFLPNYSCESVDNCPSHLNRGWRYLFYTNGGMVLVMAVLRITVIRLKETPKFLVSNNRDEEAVTILHDIANKYNRKCSLTLAELQACGTIESNDDFRKHLNILGTLKLMFDHLKILFASVKSTRSTILLFLSWAMLGISYPLYSSFLPVYLATRGANISPPEGDVAGVYRDNLIANTASIGGPIIAGLLLFYVPRLGRRGVLAIGGLSTMGFLFGYTAVRNRAGNVGLTSAIYCALYIYYGVIYAYTPEVMPSAARATGNCLCLACTRICTTIVPVIAYFSDTSSQVPMWICGAFMGMIGVLALFLPYEPSKQRAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVF6</accession>
    <name>G3AVF6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription factor Iwr1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_144096</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IWR1/SLC7A6OS family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377941.1">
        <property type="nucleotide sequence ID" value="XM_007377879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVF6"/>
    <dbReference type="FunCoup" id="G3AVF6">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVF6"/>
    <dbReference type="GeneID" id="18870597"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144096"/>
    <dbReference type="eggNOG" id="KOG4852">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044104_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVF6"/>
    <dbReference type="OMA" id="EYPRNEF"/>
    <dbReference type="OrthoDB" id="6255506at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040150">
        <property type="entry name" value="Iwr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013883">
        <property type="entry name" value="TF_Iwr1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28063">
        <property type="entry name" value="RNA POLYMERASE II NUCLEAR LOCALIZATION PROTEIN IWR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28063:SF1">
        <property type="entry name" value="RNA POLYMERASE II NUCLEAR LOCALIZATION PROTEIN IWR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08574">
        <property type="entry name" value="Iwr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor Iwr1" evidence="3">
        <location>
            <begin position="164"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="25"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="126"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="201"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="280"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="326" mass="37426" checksum="F77B86E23F784834" modified="2011-11-16" version="1">MSEQPPQILRIKRKRHQDPLQALILEDKRLAKRSKPSTPVGSPTSSPKIAPRKSQEHLNYVFKLTRTDDTVNIQDESIVNSILAESESVNTEVDARNFVIPKHQIEEDVVIPNELSDMVNSVLTFDKPTTQRKKRGHKGVQEEPAEVHPEAQEPEHDAEADDSQYVYDVYQLSMAEPLTSANHPQSLIGYIRFFDDDENDENILMNDEDNDSVKPDLTDDEDSNTESFYQNDYPSDEDAGAFSDVYDENEDEGYVPQEGVGLRDDEYFDYDEVDFHNVIDDGDDEEDDDEDTSGFKRNTFFKGDQDDPMAIYRDKIFGKLERMIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVG8</accession>
    <name>G3AVG8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">P-type Na(+) transporter</fullName>
            <ecNumber evidence="19">7.2.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">ENA1</name>
        <name evidence="25" type="ORF">SPAPADRAFT_158103</name>
    </gene>
    <organism evidence="26">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>K(+)(in) + ATP + H2O = K(+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:75815"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>Na(+)(in) + ATP + H2O = Na(+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:14634"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IID subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.3" evidence="19"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377683.1">
        <property type="nucleotide sequence ID" value="XM_007377621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVG8"/>
    <dbReference type="FunCoup" id="G3AVG8">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVG8"/>
    <dbReference type="GeneID" id="18871115"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_158103"/>
    <dbReference type="eggNOG" id="KOG0202">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002360_3_0_1"/>
    <dbReference type="InParanoid" id="G3AVG8"/>
    <dbReference type="OMA" id="FNRNPWM"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008554">
        <property type="term" value="F:P-type sodium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140115">
        <property type="term" value="P:export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000016">
        <property type="entry name" value="Calcium-transporting P-type ATPase putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000015">
        <property type="entry name" value="Sodium ion P-type ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000039">
        <property type="entry name" value="Sodium P-type ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000047">
        <property type="entry name" value="Sodium P-type ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006414">
        <property type="entry name" value="P-type_ATPase_IID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01523">
        <property type="entry name" value="ATPase-IID_K-Na"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="17" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="6"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="245"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="699"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="736"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="774"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="824"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="872"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="905"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="23">
        <location>
            <begin position="52"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase C-terminal" evidence="24">
        <location>
            <begin position="729"/>
            <end position="927"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00035017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00035029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF00689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="EGW29917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="961" mass="106436" checksum="8FE1E4F1DCC60076" modified="2011-11-16" version="1">MVIVLVISMIIALAIQDWISGGVIAFVVFLNIFVGFIQEYKAEKTMGSLKSLSSPSARVLRDGIETNINAEEVVPGDIVVINAGDTIPADLRLIDSKNLETDEALLTGESLPVAKNHEETYRSNEPVPVGDRLNMAFSSSLVAKGRAQGIVVSTGLYTEIGKIADSLKNSDHKLIVRVKKPSLKNYTKATLKSTRNIIQNVLGTNVGTPLQRKLSWLAIFLFWVAVVFAIVVMGSQKMIVNKNVAIYAICVALSMIPSSLIVVLTITMAIGAQVMVSKHVIVRKLDSLEALGGVDDICSDKTGTLTLGKMIAKKLWIPSVGTFSVQNSNEPFNPTVGEVVFSEHSPKYIKEVDEEVDFSYHLPQDSNQYFNNWLMSATLANIASVKEVDGEWQALGDATEIAINVFTTRLGWIRDEVENINELKHLNEFPFDSAIKRMSAVYEANGEVKIYTKGAVERLVNICNTWYVDGKLVPLTEEDHKTIEDNMTVLSSQGLRVLAFAQRHLRSVDEVDIQNRDAVESELTFLGLIGIYDPPRPESAKSVQLCHKAGISVHMLTGDHPGTAKAIAQEVGILPHNLYHYSRDVVEVMVMTAMDFDALTDDQIDALPVLPLVIARCAPQTKVRMIEALHRRGKFVAMTGDGVNDSPSLKKADVGIAMGMNGSDVAKDAADIVLTDDNFASILNAVEEGRRMSSNIQKFVLQLLAENVAQALYLMIGLVFIDEDGFSVFPLAPVEVLWIIVITSCFPAMGLGQEKAQDDILELPPNNKIFTREVLVDMFAYGTWMAACCLAIFVITVYGKGDGDLGHNCNSHPEHCSLVFKGRSAAFVAFTWCALLLAWECVHMRRSFFLMRPESELTWYHQLWLDVWENKFLFWSIIGGFVSVFPVVYIPVINDKVFLHGPISWEWGLAIGFTVLFLAGSEAYKWLKRIYLRKQKAKDAFADLSVFERYVSLSKSNTMDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVP5</accession>
    <name>G3AVP5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_53032</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377976.1">
        <property type="nucleotide sequence ID" value="XM_007377914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVP5"/>
    <dbReference type="FunCoup" id="G3AVP5">
        <property type="interactions" value="1123"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVP5"/>
    <dbReference type="GeneID" id="18871748"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53032"/>
    <dbReference type="eggNOG" id="KOG2274">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008920_1_1_1"/>
    <dbReference type="InParanoid" id="G3AVP5"/>
    <dbReference type="OMA" id="NPDQYTI"/>
    <dbReference type="OrthoDB" id="431626at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056840">
        <property type="entry name" value="HEAT_IPO9_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF9">
        <property type="entry name" value="IMPORTIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25018">
        <property type="entry name" value="HEAT_IPO9_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="6">
        <location>
            <begin position="23"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="945"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="952"/>
            <end position="969"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1037" mass="117046" checksum="B54AD0E543CDE0EE" modified="2011-11-16" version="1">MSDLLITLILNQTNSDNNIRTAAELEFNQFLKQDPTNAAYIILESALNQDYPIDLRQSCLLHLKRLVPYYWSMGFQSFIGPPIAQDVKVKIRQSLLELATSSPESKLRNGSAYAIVQIASADYPDEWPELLNVLYSATMNFSNENSLIGGLQVLTDLFDDLITEEQFWEGGIAGQVTSHINDILTGDLSSEVKTQAIKLYSNVVDILSSPEAFSKPPRKQFVINHVASSIQIFAQLLEKSYNNSTGNTSTVYLSELNLRTSIYRILNLLLGQFHKKITQETKANLLSLTIQDFNYVAQLFANFVVVENTANVPEIKTTSDLTSISSILSNLLEELLVTVSNCQLDISVGEVAPHEQFFKDLVTVSSLTQSTIEEYQADINVYASDLTGLSTVSRVRETVNDVLSELNESDSSQVYKRVIEGLAGGQGDWRLIEAQLYILESLFINDDVSFDQLELSPPDLLNLFARYISYDKPLVTARSFILLPKFFEKFSNIPKETRRKAFSDMIVFTIGLLDKDFEACDFIKIACLVSITYYKHVIDFNHDLAPQGKDIQTAIFTIVHSLIEESEEDGLPSLLEAITEAISIDPQQATTIQIGNDINVIDLIFKITFKDPANVQLIVDTSDCLTSLLKNISIDNYLQTCEKSLPVIFEIMNQEIKRNNVEVEYTPELYLALELLSIIIESVPESQQELPSSIFYYAFPILRRILTLSQDNQILQSGGEVFNNIIQKASKLFLEYQDPETKQSGIDCMMEIVYKFLSPELSDSAANNCGSIVYSLIDQFQSYLTSDLLTQILKATVLRLSYAKESITIENLIMVFCKLIINSPQEMVDFLSNNISLTIDGVAKSGLEIILPIWFESYEVTRGYEHIKQNSLALGKIFSLGDARVENLVVNGEIIPYEGDLIITRSMAKAMPDKYTQIPACLKILKLLVGELEFQCQQPNAEDYLPEREELGEADNDDDDDEEGWEDMEDIGVPNFEKLKSYVDSDAEGDRDSDESLKHLLIQFLRECTVKDLGHFRKYYEELNDQEKKTITENLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVU7</accession>
    <name>G3AVU7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_143912</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377758.1">
        <property type="nucleotide sequence ID" value="XM_007377696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVU7"/>
    <dbReference type="GeneID" id="18870584"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143912"/>
    <dbReference type="eggNOG" id="ENOG502RPTN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_449478_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVU7"/>
    <dbReference type="OMA" id="TIQTTGH"/>
    <dbReference type="OrthoDB" id="4022940at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW29992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="591" mass="65837" checksum="0A37FC181243E6CE" modified="2011-11-16" version="1">MEESNPSQSFYIQTNGSSSSRIHLPGVNKPSANVSSVGFNTLKKMATNSSSSSITTSSSTSRTNPLTKLFSKNKSVSSLLPQATSELSLKEGDDQSFLNDSNSSMLTYDSKKGGTNKFRISRRLKFKNKGKPDLTIQTTGHHGIRLPKKIVSSTSLNESNSGGTRKNSLGSPVSTFHSFFHRSHALSSPLEPTNSKAKIDEHTNNTRSALCLSSNSSNSYIADIKLASIYKFTDPNYSIDETEGTTDYALHKKMLLPADSFFQSRAHKNSQDGTIPVSDDSETESIPAISDFSKTNARFLGNLMNVIKPLFLVPIRKNSEAIIIHPYLGYSTEDIASFIKENLFEESKHSPETSISSPNKSKPKHKTGLNNLLTLPNDSAEDLDYFRAREISQDVQTLFIKCLTIFRKDITNMNFNDSKSKLPPPSTKGLVTDLSVSWQEISFGWTHFNKKIRFYILSLFEPLQGCFDTVSQYKFNSSSDFPPVNFEKELILAFRDAILVPFLLERKQKYFEFTGEDTVSDPESSYHTLFLDEQAFLKENSHLVSVLIDCFASISCHTLSGNGSLTADGEQFYVTEIIRKTFTWLSSLSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVX6</accession>
    <name>G3AVX6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mitochondrial adapter protein MCP1 transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_63639</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377787.1">
        <property type="nucleotide sequence ID" value="XM_007377725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVX6"/>
    <dbReference type="FunCoup" id="G3AVX6">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVX6"/>
    <dbReference type="GeneID" id="18874845"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63639"/>
    <dbReference type="eggNOG" id="ENOG502S974">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066681_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVX6"/>
    <dbReference type="OMA" id="HLLIMKW"/>
    <dbReference type="OrthoDB" id="10259513at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03493">
        <property type="entry name" value="SQR_QFR_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039960">
        <property type="entry name" value="MCP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012472">
        <property type="entry name" value="MCP1_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38409">
        <property type="entry name" value="MDM10-COMPLEMENTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38409:SF1">
        <property type="entry name" value="MITOCHONDRIAL ADAPTER PROTEIN MCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07950">
        <property type="entry name" value="MCP1_TM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Mitochondrial adapter protein MCP1 transmembrane" evidence="2">
        <location>
            <begin position="56"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Mitochondrial adapter protein MCP1 transmembrane" evidence="2">
        <location>
            <begin position="184"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="302" mass="34219" checksum="A13EDE201BB2A876" modified="2011-11-16" version="1">MPEELHKINPIPLDILEEIQANELTPTPSQPTHKKPWLFPILYKIQKYSAYSFVSFVGIHLTSVVIVPILPIDTGIKQEVFSLAKAVYHALPLYETTIIYGSSLLHVISGVTLRVLRTYERQQKKKNKKTGHFNMNDSIQIKDSDDDIGLGGISSIFGLGYKKSWISQTFGVSPLQFSGYILIPLVAYHYFKFRLSPLLVEGDSSLINLDYITYMVNLRNPTANLLALSGLVWTATYHVCNGLLKLNHKFNKNWKRFGLVMINAIGSLGMLAIYYYKIDTDIINVNGYLGKSFASYINSFWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADY1</accession>
    <name>G3ADY1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-deoxy-7-phosphoheptulonate synthase</fullName>
            <ecNumber evidence="4">2.5.1.54</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">3-deoxy-D-arabino-heptulosonate 7-phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">DAHP synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Phospho-2-keto-3-deoxyheptonate aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_57769</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-I DAHP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="4"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372117.1">
        <property type="nucleotide sequence ID" value="XM_007372055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ADY1"/>
    <dbReference type="STRING" id="619300.G3ADY1"/>
    <dbReference type="GeneID" id="18872188"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57769"/>
    <dbReference type="eggNOG" id="ENOG502RXNY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033451_0_0_1"/>
    <dbReference type="InParanoid" id="G3ADY1"/>
    <dbReference type="OMA" id="NLTKYNH"/>
    <dbReference type="OrthoDB" id="4020666at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006219">
        <property type="entry name" value="DAHP_synth_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE DAHP SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225:SF12">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE, TYROSINE-INHIBITED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP synthetase I/KDSA" evidence="12">
        <location>
            <begin position="203"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="426" mass="47410" checksum="87993F542C853648" modified="2011-11-16" version="1">MSLQPRIKSPLSRTLEKSLVIETSESVNTTDTEVESPVAHSYAPEWEYHQTATKSSNTTNKGNIPIPQPEVMQKVLYPITPQLQNKIFNDRTNVNSLLSDGKKPLLVITGPSHIKDTNQIKACAQYLSLKQGNSITSSDYLPSEIKSIFHDPAELSNLHLALRTNLTMYNHAYTSPQSQSIRTFEIDKGIPKCRSLLRELSQYAPLVSEFVDTLTPQYLADFYSMGIVGPTLIESQLHRELASGSSYAVGFSAGEKSDMLDHKLTSCLDSMYATSQPHQFLSITKYGTVAVVGTTGNSDTFVILPLGDDITELVAKVNSYKNLQGKTVKLLLDIGRVSNDDYETKLITLKSVLDSEVRDQILGVMIDSGDHYVPYDYQVDDREDVESLFTLVDRASSESLSFAPNSNYEYFINANNLLLELNNLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE30</accession>
    <name>G3AE30_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Salicylate hydroxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_64670</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the paxM FAD-dependent monooxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372976.1">
        <property type="nucleotide sequence ID" value="XM_007372914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE30"/>
    <dbReference type="STRING" id="619300.G3AE30"/>
    <dbReference type="GeneID" id="18874991"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64670"/>
    <dbReference type="eggNOG" id="KOG2614">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_19_3_1"/>
    <dbReference type="InParanoid" id="G3AE30"/>
    <dbReference type="OMA" id="MTGWMGP"/>
    <dbReference type="OrthoDB" id="9993796at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000115">
        <property type="entry name" value="Salicylate hydroxylase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050493">
        <property type="entry name" value="FAD-dep_Monooxygenase_BioMet"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13789">
        <property type="entry name" value="MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13789:SF147">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_2G01950)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="6">
        <location>
            <begin position="7"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="416" mass="46077" checksum="386E745C741B75CB" modified="2011-11-16" version="1">MVMVSFNVIICGGGLGGLACAIGLKKKGHNVTVLEASSTLSEVGAGIQVPPNSVKILKEYGIFDKFEKLVTRPKNIILRRYDTGAPLSTTPLDPDMTETYGNPYLLIHRADYQRFLLEAATELGITYKTGCRVKEVDPETATVHLESGESYTGDVVVGADGIRSKVRDTAVVTEEVVSPLPSSNCAFRATVPKEEMLSDPVIAHLMTDVNSNCWIGYRRHVMAYPIRNGEMYNIVMSHPGQASVGKWNEPGDVNEMRNHYKTFDPVVRQLLTHVKSVLKWVLADLPKLPRWVSKSGKVVLIGDAAHAMLPYLAQGAAQAIEDGATLAEELDKITSVSEIPTALLSYQKLRKRRPETIQAGARKNGDIWHLPDGEEQEERDALMNARDGHNPDQWSDREFQQWLFGWDAFTSTYPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE42</accession>
    <name>G3AE42_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">CFEM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_133039</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RBT5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372988.1">
        <property type="nucleotide sequence ID" value="XM_007372926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE42"/>
    <dbReference type="GeneID" id="18869711"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133039"/>
    <dbReference type="eggNOG" id="ENOG502SD7M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_941850_0_0_1"/>
    <dbReference type="InParanoid" id="G3AE42"/>
    <dbReference type="OrthoDB" id="2496787at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051735">
        <property type="entry name" value="CFEM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008427">
        <property type="entry name" value="Extracellular_membr_CFEM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37928">
        <property type="entry name" value="CFEM DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G14090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37928:SF2">
        <property type="entry name" value="GPI ANCHORED CFEM DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G10580)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05730">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00747">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52012">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="6 15" id="KW-0349">Heme</keyword>
    <keyword evidence="10 15" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003441964" description="CFEM domain-containing protein" evidence="17">
        <location>
            <begin position="20"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="CFEM" evidence="18">
        <location>
            <begin position="33"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="CFEM" evidence="18">
        <location>
            <begin position="153"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="286"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="73"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="82"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="193"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="202"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="19">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS52012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW35576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="296" mass="31287" checksum="B43E2552787AB30B" modified="2011-11-16" version="1" fragment="single">MKSSALALLIISIAYGVQGDEITRVPRAAETENTSSTLPTVVKTATINGFADKIYDDLPECAKACVVQDVGRCPYWDTGCLCVMGTFANPVANCFADECKGQDVVSVTSLAFSLCSSAGATWNLPATIAERLDAAATVEPEITNVPDSSSTNNPYSTFPSVVKTATINGFADKIYDDLPECAKPCMTQDVGRCPYWDTGCLCVMGTFANPLADCVADECKGKDVVTATSLAYSVCSAAGVWEPYWIIPTSIIERLDAAATEEVEEVITTEVTIITTETSEGPKEISASSAEPAETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEQ9</accession>
    <name>G3AEQ9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pre-rRNA-processing protein RIX1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_130672</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the RIX1 complex required for processing of ITS2 sequences from 35S pre-rRNA and the nucleoplasmic transit of the pre-60S ribosomal subunits. Regulates pre-60S association of the critical remodeling factor MDN1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the RIX1 complex, composed of IPI1, RIX1/IPI2 and IPI3 in a 1:2:2 stoichiometry. The complex interacts (via RIX1) with MDN1 (via its hexameric AAA ATPase ring) and the pre-60S ribosome particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RIX1/PELP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373097.1">
        <property type="nucleotide sequence ID" value="XM_007373035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEQ9"/>
    <dbReference type="FunCoup" id="G3AEQ9">
        <property type="interactions" value="326"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEQ9"/>
    <dbReference type="GeneID" id="18869513"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_130672"/>
    <dbReference type="eggNOG" id="ENOG502R65X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020084_1_0_1"/>
    <dbReference type="InParanoid" id="G3AEQ9"/>
    <dbReference type="OMA" id="WCGINLI"/>
    <dbReference type="OrthoDB" id="20900at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012583">
        <property type="entry name" value="RIX1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34105">
        <property type="entry name" value="PROLINE-, GLUTAMIC ACID- AND LEUCINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34105:SF1">
        <property type="entry name" value="PROLINE-, GLUTAMIC ACID- AND LEUCINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08167">
        <property type="entry name" value="RIX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Pre-rRNA-processing protein RIX1 N-terminal" evidence="10">
        <location>
            <begin position="5"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="432"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="584"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="672"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="432"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="584"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="628"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="693"/>
            <end position="716"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW35685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="716" mass="80641" checksum="BA63B17B5AD0DB71" modified="2011-11-16" version="1">MSIPISVILEELKTTPTSVIPLLRSLHNKTLLQSLSKSEITHLTSRILNLAKSHQPYPQWCGINLIRVISSDYSILAGYGSIFLDQLLKLVENSQGKILVNCIDVIGFICSEIRGKPGLTREILTPKLGPIISLFIEKLHLAPFEILKNLVVAIRFHPTTFRPYGNKLSAKLVRLVNDVGFVNYPSKLKDVVYEALAVLPGIEKSEPEQKWSNDVFGLIGEIKDVVEVFQEFVHFKDDQDLIDNLRKLEKSGKTESKMFEDLNIDLNSPNSIFQLSNRIDISLGLLKSYLITESQFTVKVPLGTIITVCEVLTGLNLRMLRFKGDIRDEDVKSIIRSSIANIHHSVVLFLSDIVDVYKGALLLHFHSILGMLEVIIPMKGNKVSYDDLISNEGLIIDLLIFVAKYVSLVKHLDDNTQLLPFVDVSMILVEPRSKPQKEKEQKQDGQKKNKKQKKKGNAAALSDILSHEHLFLESIPASTVSAVRKFINVIIKTITLSPSQHYKVLRYLIIESINIRHINQQHSVPQELKQLLIDCVLFPGYDRVNILPIVSSILKGDQLLSVFNNPRFPPLPRYVRQEELVQQDLDEEEEDEEVEETNDIGSKKRKLDELMAEAGNPVQDNEPEPEQDDSKLFKKSKQEVNEVETNTLEEVVQDKVPVKETPKAVEEIVETVSASVPVETVPASSSTISHADDDSEEEGDEIEIPELDLGESSDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AER1</accession>
    <name>G3AER1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anaphase-promoting complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_58893</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the APC5 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373099.1">
        <property type="nucleotide sequence ID" value="XM_007373037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AER1"/>
    <dbReference type="FunCoup" id="G3AER1">
        <property type="interactions" value="161"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AER1"/>
    <dbReference type="GeneID" id="18872663"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58893"/>
    <dbReference type="eggNOG" id="KOG4322">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020870_0_0_1"/>
    <dbReference type="InParanoid" id="G3AER1"/>
    <dbReference type="OMA" id="MARENND"/>
    <dbReference type="OrthoDB" id="2504561at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037679">
        <property type="entry name" value="Apc5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026000">
        <property type="entry name" value="Apc5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12830">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12830:SF9">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12862">
        <property type="entry name" value="ANAPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 5" evidence="7">
        <location>
            <begin position="88"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="564" mass="65386" checksum="E625B46B5801155F" modified="2011-11-16" version="1">MYLSMGGAIGDTFDSEDNSELFSRLNQQLTEANIDSMSNSTKEIASESDLSLILNKQIELLESKGISPPELLDRIFTSSTVANLPSYYYINYLESLKVYDYNSSFESLYRYFDYMVSNNSKYFYHFALILKANLHQYYNQHELAVNSIQEAISIARENKDNSTLTYIISWLFNYIKDKPELVGDTANPNQLLEFLIKKTKSVSLSLYSLSYNFKTFQVLNHGGGIRTYLNDLTKATYISIYDEFQITSFIKTMEMGVIVWNRIGNNILSEVYNDIAIEFTNNSNDLISLKIRLNFLKFLQGDIESSYNELQELKKSVQHDYSLFNSIQIRSLIMLIKINLFKGKFVQAKEIVVTLLDNDLQDIELIHEFIQLQVEIEIYLENYSRALKLIEKHQHRSNTYLMIRLKLLKCAIFNKSNNHIKSFSLIIQTIQLSHKCGYANLLMEALIIFINILNHLGYFQDVLDIFENIMPALISLNSQHLTSQAYFGLAHAYFNNYKKTQQEALFNNVLKFLNKSIKGFNSCNDLINVRSCSEFEIEIAKYSQDHELLVHARENLDRINTLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AES1</accession>
    <name>G3AES1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58900</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373109.1">
        <property type="nucleotide sequence ID" value="XM_007373047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AES1"/>
    <dbReference type="STRING" id="619300.G3AES1"/>
    <dbReference type="GeneID" id="18872669"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58900"/>
    <dbReference type="eggNOG" id="ENOG502S1KC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1180849_0_0_1"/>
    <dbReference type="InParanoid" id="G3AES1"/>
    <dbReference type="OMA" id="NTRFRFY"/>
    <dbReference type="OrthoDB" id="5374328at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="235" mass="27295" checksum="4B6C358D83071C42" modified="2011-11-16" version="1">MKNIKTIDSENLSLKSTLLSLIHDYKQLKNLVLNPEVITSVHQPLDSNVHKRSYTEVKTNEVLTTSTTTEFDKFIDLDNNDHVDLKKSKTNDYFEPIDSDIEIDYEDDYEYNMMTSPTLSSTELSRTTSPYSDFENNSLMSSLTRSTTVSSVMSFDKPKFTLNNFFELPKYEEEQDDHEYNFKFDELMNNNSKNDQYNMITDFLEEKLITNDIEYYTASTTTAPVASSSDNNLIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEX6</accession>
    <name>G3AEX6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LicD/FKTN/FKRP nucleotidyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_48767</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373218.1">
        <property type="nucleotide sequence ID" value="XM_007373156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEX6"/>
    <dbReference type="GeneID" id="18871264"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48767"/>
    <dbReference type="eggNOG" id="ENOG502QREF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008074_2_0_1"/>
    <dbReference type="InParanoid" id="G3AEX6"/>
    <dbReference type="OrthoDB" id="444255at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009644">
        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF28">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE TRANSFERASE FKTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="6">
        <location>
            <begin position="400"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="747" mass="87463" checksum="5485B7D813CBA380" modified="2011-11-16" version="1">MFKNMNINKLFKIIPIVLCINTIGILLYYNSHLRHIANNLDYVNALPQTGFTSPEITKKLKSILSFKPHKVTSEVTIPTDYFETEELVQDPRVTFAITLNWIYHQIKVDPENVSFPFNWADWVDLTYLNHQISKPVNEKIKCSDLIEHIHFNTPDDKAKSIADPMFFGCKNTQDLTEKEMEEMGLTNLDRMPGFFQFYHTVFKPSEFIRMLQGKTYLLSKMPLPHQVIFLNDAGDDLTFQVDGKTTARELLKTYITNNSLEKNKIITLDPIKEFQQLLDLQGANTYENLYDADKIYHMSRSWFHYGSDNVTNQIERLTSQEELTPIERGYLTSLIISKEASEKKPHNEPMFFNTGTFRKTSMNNDDGGHYDWRFINGRWRDRYRHAILLERLLRSWFKFCQKNGIVSWINFGSLLGWYRNGAIYPFDLDMDIQMSMYHMTILGKKFNQTLVVEDLHEGTGKYFIEVGTFIHNRNKIGRYLNHIDARLIDADSGLYIDLTALATETKYSDVHPKFFKDICDDRVEGPVLEDDGDTEVYNDRNDWVYKFGNLSPLRLTFFEGVPFYVPKQIVKRMKFQYPCGTLNNFEFKQWYYIEQVGTWIHEKELFAVLNVAEIKKKGKINIDKVKKQVENLTDEQLYTLISNDPATLSNYQLARRTSGFHTKEHQYLFTIDPELHKGINDPPEGKVLDASPEENPEYLKLIEDNVWLRAPHRESIFEYERVKGMYSEFNELALKELDKIAVSKSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEY1</accession>
    <name>G3AEY1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar-sorting protein SNF7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Vacuolar protein-sorting-associated protein 32</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Vacuolar-sorting protein snf7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_57850</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372197.1">
        <property type="nucleotide sequence ID" value="XM_007372135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEY1"/>
    <dbReference type="FunCoup" id="G3AEY1">
        <property type="interactions" value="673"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEY1"/>
    <dbReference type="GeneID" id="18872222"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57850"/>
    <dbReference type="eggNOG" id="KOG1656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071097_1_0_1"/>
    <dbReference type="InParanoid" id="G3AEY1"/>
    <dbReference type="OMA" id="MKQIHGG"/>
    <dbReference type="OrthoDB" id="5592979at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904669">
        <property type="term" value="P:ATP export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070676">
        <property type="term" value="P:intralumenal vesicle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1060:FF:000012">
        <property type="entry name" value="Vacuolar sorting protein SNF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1060">
        <property type="entry name" value="ESAT-6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF10">
        <property type="entry name" value="GH13992P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="167"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="21"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW34785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="222" mass="24928" checksum="35E25D3A9495E97C" modified="2011-11-16" version="1">MWSLLFGGNSQAKKDLPKKAIVELREHIQTLNKKKSHLEQQMADQDAMARKYVASKQNNLAKNALKRKKGYEANLMKVENQIETLETQLISIEGANLNLETMKAMKQGASAMKQIHGEYNVDKVEDTMDEIREQVELADEISEAISRPVGNEFVDEDELEEELAALQEEQAEQEAAPVQKVPAQKVEQLPEFPTVNKTVPAAPVEEDEDEEALKALQAEMGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF03</accession>
    <name>G3AF03_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RNA-binding protein VTS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_57886</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Monomer. Binds to RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VTS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372219.1">
        <property type="nucleotide sequence ID" value="XM_007372157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF03"/>
    <dbReference type="FunCoup" id="G3AF03">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF03"/>
    <dbReference type="GeneID" id="18872237"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57886"/>
    <dbReference type="eggNOG" id="KOG3791">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_443421_0_0_1"/>
    <dbReference type="InParanoid" id="G3AF03"/>
    <dbReference type="OMA" id="LHKYTDC"/>
    <dbReference type="OrthoDB" id="2155283at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09556">
        <property type="entry name" value="SAM_VTS1_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050897">
        <property type="entry name" value="SMAUG/VTS1_RNA-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037635">
        <property type="entry name" value="VTS1_SAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12515:SF5">
        <property type="entry name" value="PROTEIN SMAUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12515">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN CONTAINING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="SAM" evidence="9">
        <location>
            <begin position="591"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="97"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="188"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="384"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="480"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="118"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="191"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="397"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="482"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="551"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="654" mass="71931" checksum="539311A1AEBECD02" modified="2011-11-16" version="1">MDQQSSNKSPNPYSETPIILSPPPLEQEQQRHIQQQLQSHSQKPQPMSLPPFAQHTAAPSSNQLPQGHSERQQSVGYNLQHEFETLTADLDLDLRNNLPTHYENQPPSKPPLEVPQPQHLSASSVNETSLKSPGLMAPSAKYGALGADLLGISSSGSSSALLRDSALSPIPQIPSANLASLLKPNGVSSYNSQNSSTTPGNTSTGFLGPPPLASIPDRPQSVNDFSNIFNRNELNSFQSYAQPQQAPQPQQQQQLGQASFYSDLLVFSNWIENLNPQDNITMIDYLCNSLPLDILLAFKSRLDHHLQAPKLQTQPAQAQAAQQQNFNLMSPLNNSYSQDLYTDIEQLNLQDSKLQQQPQQLQHPKPTFRQGAQYLNIMDRIARPKSAEPSASGIRYGNSQQQFERSRSPTSHLYEKTNFLQLAAVTNAPVRHHYGHDDDLSSHTALKLGALATINSRVALDSNRKHGHSHGATGATWNLTMQQQQQQQQQQQQQQPQQPQPQHKHTQSRTHAAVAYEDSLNRSLNSASVPANLHRTTSSKSSSSKKKGPQSPTSNNTSTTAATAAAAGSTTPSTTTSSSMPSEVANIELLNNIPAWLKLLRLHKYTECLKDIPWKELIELDNDELEAKGVAALGARRKLMKAFDAVKASLNEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF83</accession>
    <name>G3AF83_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD-dependent protein deacylase</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Regulatory protein SIR2 homolog 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_57964</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N(6)-glutaryl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-glutaryl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:11875"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:87828"/>
            <dbReference type="ChEBI" id="CHEBI:87829"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N(6)-malonyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-malonyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:11878"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:87831"/>
            <dbReference type="ChEBI" id="CHEBI:87833"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N(6)-succinyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-succinyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:11877"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:87830"/>
            <dbReference type="ChEBI" id="CHEBI:87832"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">In contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-77 and Arg-80) that bind to malonylated and succinylated substrates and define the specificity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sirtuin family. Class III subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372284.1">
        <property type="nucleotide sequence ID" value="XM_007372222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF83"/>
    <dbReference type="STRING" id="619300.G3AF83"/>
    <dbReference type="GeneID" id="18872272"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57964"/>
    <dbReference type="eggNOG" id="KOG2684">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023643_3_1_1"/>
    <dbReference type="InParanoid" id="G3AF83"/>
    <dbReference type="OMA" id="KWIAAGP"/>
    <dbReference type="OrthoDB" id="424302at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061697">
        <property type="term" value="F:protein-glutaryllysine deglutarylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036054">
        <property type="term" value="F:protein-malonyllysine demalonylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036055">
        <property type="term" value="F:protein-succinyllysine desuccinylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01412">
        <property type="entry name" value="SIRT5_Af1_CobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01121">
        <property type="entry name" value="Sirtuin_ClassIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027546">
        <property type="entry name" value="Sirtuin_class_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF10">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 4" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="6">
        <location>
            <begin position="8"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4 5">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="117"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="254"/>
            <end position="256"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="317" mass="35106" checksum="052ACCFC7145AF47" modified="2011-11-16" version="1">MRPSESPSTTMSTQLKEFQEHLLKSKKIVALVGAGLSVSSGLPTFRGSQGLWKNFNMIDLATPDAFYIDPGLVWQFYSWRRYTALKAKPNKGHYALAKLSQMGKSPQSDLTYITITQNVDGLSSRSGHCKENLYEIHGSLFDLKCTSFMCNFVDHNNFKQPLTKALADTEEEFDPSHNRKRSLDEQEVSISPQFNPVKTLSEKELPQCPVCKDGSLLRPGVVWFGESLPLSLITKVDNFIESEGSPVDLILVIGTSGTVYPANSYVDRVKLKGGKVAIFNTDIENDIVVGKVKDTWGFKGDAAELLPLALKPLIGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFB4</accession>
    <name>G3AFB4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_58028</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372315.1">
        <property type="nucleotide sequence ID" value="XM_007372253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFB4"/>
    <dbReference type="FunCoup" id="G3AFB4">
        <property type="interactions" value="947"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFB4"/>
    <dbReference type="GeneID" id="18872290"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58028"/>
    <dbReference type="eggNOG" id="KOG2496">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022620_4_2_1"/>
    <dbReference type="InParanoid" id="G3AFB4"/>
    <dbReference type="OMA" id="KSIMACA"/>
    <dbReference type="OrthoDB" id="340962at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070985">
        <property type="term" value="C:transcription factor TFIIK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905866">
        <property type="term" value="P:positive regulation of Atg1/ULK1 kinase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd20524">
        <property type="entry name" value="CYCLIN_CCNH_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20525">
        <property type="entry name" value="CYCLIN_CCNH_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031658">
        <property type="entry name" value="Cyclin_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16899">
        <property type="entry name" value="Cyclin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0195">Cyclin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="4">
        <location>
            <begin position="19"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="202"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="221"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="303" mass="35465" checksum="60B4D6CA040E0075" modified="2011-11-16" version="1">MISLLTPEEEATYLDFYSNNIIKTCNFFKMPTQVKLTAASFFKKFYLVNSVMEYHPKNILYTCVFLAAKSENYFISIDSFVRALQGTEAAHILDLEFIVLQSLKFTLLVHHPVRPLYGFFLDFQAVLLHPEPVMYDVSVDTLGNLYNRAKDWLNEHAVTSDVTFLFTPPQIALAAMYDTDKRITDRYLKRKFLKEDKEKLNVIKEGDEEQPDQNTEPQNEESEKQGVVKEESETNSHHNIKLHTQREHYETLVRTIRKCIKIAKEVKPTTREESKEIDKKCFFALNPKKLIDKRIKRLALEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFH9</accession>
    <name>G3AFH9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DASH complex subunit SPC19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Outer kinetochore protein SPC19</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_58091</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="16">Component of the DASH complex consisting of ASK1, DAD1, DAD2, DAD3, DAD4, DAM1, DUO1, HSK3, SPC19 and SPC34, with a stoichiometry of one copy of each subunit per complex. Multiple DASH complexes oligomerize to form a ring that encircles spindle microtubules and organizes the rod-like NDC80 complexes of the outer kinetochore. DASH complex oligomerization strengthens microtubule attachments. On cytoplasmic microtubules, DASH complexes appear to form patches instead of rings.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex SPC19 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372380.1">
        <property type="nucleotide sequence ID" value="XM_007372318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFH9"/>
    <dbReference type="STRING" id="619300.G3AFH9"/>
    <dbReference type="GeneID" id="18872317"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58091"/>
    <dbReference type="HOGENOM" id="CLU_1732614_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFH9"/>
    <dbReference type="OrthoDB" id="3361333at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013251">
        <property type="entry name" value="DASH_Spc19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28262">
        <property type="entry name" value="DASH COMPLEX SUBUNIT SPC19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28262:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT SPC19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08287">
        <property type="entry name" value="DASH_Spc19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="14" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW34968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="151" mass="17713" checksum="387FD06488E46BCD" modified="2011-11-16" version="1">MPFDILSNSIDSLRDSVVLLQKCDGLLQTTINKVSYRNKIATRQVFGLTTQSDLNDAKLHFRNNIHQQLRKNTHTINHLMQELTRRKSSLENLRVQQSKVIDHYDDVDDLIKQFEKSKTVDRLKLRKLIMLKEKKERLKYTLARSSGSTFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFT9</accession>
    <name>G3AFT9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13">Amino-acid acetyltransferase, mitochondrial</fullName>
            <ecNumber evidence="5 13">2.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Glutamate N-acetyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">N-acetylglutamate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_132397</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 13">N-acetylglutamate synthase involved in arginine biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>L-glutamate + acetyl-CoA = N-acetyl-L-glutamate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.1" evidence="5 13"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372490.1">
        <property type="nucleotide sequence ID" value="XM_007372428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFT9"/>
    <dbReference type="FunCoup" id="G3AFT9">
        <property type="interactions" value="108"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFT9"/>
    <dbReference type="GeneID" id="18869655"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132397"/>
    <dbReference type="eggNOG" id="KOG2436">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013088_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFT9"/>
    <dbReference type="OMA" id="NAMVRDC"/>
    <dbReference type="OrthoDB" id="5585968at2759"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00106"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004042">
        <property type="term" value="F:L-glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006592">
        <property type="term" value="P:ornithine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011190">
        <property type="entry name" value="GlcNAc_Synth_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006855">
        <property type="entry name" value="Vertebrate-like_GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342:SF4">
        <property type="entry name" value="AMINO-ACID ACETYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04768">
        <property type="entry name" value="NAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007892">
        <property type="entry name" value="NAGS_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51731">
        <property type="entry name" value="GNAT_NAGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7 13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="14">
        <location>
            <begin position="402"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR007892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW35078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="581" mass="66968" checksum="E86B616E24BD7B82" modified="2011-11-16" version="1">MSKWQQFQRQFVSNLEKQKSTTDAKRNLILSILKSTTTKREARNYLNKYQSQFDFSGLDFNKSIKTINDEQSLTKRDTQRGLFITRYLNNQNPFVNIYDKEDVKQKKVPLRIAIFQIKYPKVTYQQWKGIAETFKRLLTLGISPIILLDYDHFLTDSFKLNEQYMIEAASKLLTYFGRPEEESELKAIVLRSLFTNRNGKLSIDSLESVLIPMYQGLVPIIQPIAYESQSAMQEFISTDQLLYSLSSALVEKSTSDILTIEKIVMIDPMGGIPSIERNQSSHVFINLCQEYSDILSELYIGHIEPKVRDFHVSNLDSMNTVLSYINDRTGNDETTGIITTPEIMSVNHDELNPIIYNVLTDRPIISSSLPSTNTRTPQLSTTIIKKGVRVDIYDQDNYPDKFTLQNLFRDNLIDKDRLIELMNDSFGKPLDSETYINRINENLATLVIVGDYDGAAIITWEYSQGEKIAYLDKFAIAKKNQGLPGLADIIFKIILSSHPVELIWRSRKVNPVNKWYWERCCGCMSSPESQWKIFYTGEIFDKKIDKRKRSVHGLDISKKLQQYSEICEGIPPSFVSVPRVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFY4</accession>
    <name>G3AFY4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Plasma membrane fusion protein PRM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_53463</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Involved in cell fusion during mating by stabilizing the plasma membrane fusion event.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cell membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the PRM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372535.1">
        <property type="nucleotide sequence ID" value="XM_007372473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFY4"/>
    <dbReference type="FunCoup" id="G3AFY4">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFY4"/>
    <dbReference type="GeneID" id="18871785"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53463"/>
    <dbReference type="eggNOG" id="ENOG502QRP5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010191_1_0_1"/>
    <dbReference type="InParanoid" id="G3AFY4"/>
    <dbReference type="OMA" id="NVFGWVN"/>
    <dbReference type="OrthoDB" id="5356111at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032220">
        <property type="term" value="P:plasma membrane fusion involved in cytogamy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026777">
        <property type="entry name" value="PRM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31030">
        <property type="entry name" value="PLASMA MEMBRANE FUSION PROTEIN PRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31030:SF1">
        <property type="entry name" value="PLASMA MEMBRANE FUSION PROTEIN PRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0184">Conjugation</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="545"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW35123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="627" mass="71167" checksum="4059680F7E7D9321" modified="2011-11-16" version="1">MSTPITRNYLNLTEILSQAYLNKYTILFLLILVKLIIFKNSLISQLNQTIVNETICNKDQIEPILSSIHNIILDGLKETQHAGITFIVIIVTIIKNLLIFYIEIFVGTFLCLLNAVIKGTTELALDSSETIIRAVNTTVVEVTNQVEEGLKGLSGVLNDFAKGAMGFKSIFSGKASNPSEFEDRIHLSLGELKDKIAIPSEVVDAINSVRNADLGKLTKLDNATQVILEAPFKLVTKQLKEFKDKKNYSETVKPIDANQIQQLCNAHVESVHNVQKGLIIVVENLTKTLVILLIMAMIGSTCYVVFIECMKWRRMQKFIREDLSHPVKFKNQYNIYHSSSMYMVAKRFNLRVGDRALWWWSFLSSKFAGYTLLFGIMGLMALLCQYIIISKLRHTLGERATELMQDEQSRNLTTTYIKDMKQYINNTESSVNKELFGHFHKATGAVHKELTEFIEIMNSTMTDIFGKIPLGKPINTVIYCVLGRKLVALERGIDWLHQNIRIKLPRLSKNLENDIKQLKFVKTSSVLNKLNTMIDVYEKSLRLELYISTVLVALWGLQIIIGSIWVFLQDRKVTQELVMISSPQPLSKSEKILYGYPLDNPHISFPGAFIPSRSSSIPSPLSKYNKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG72</accession>
    <name>G3AG72_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BBC1/AIM3 cysteine proteinase-fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_58411</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372623.1">
        <property type="nucleotide sequence ID" value="XM_007372561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG72"/>
    <dbReference type="STRING" id="619300.G3AG72"/>
    <dbReference type="GeneID" id="18872441"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58411"/>
    <dbReference type="eggNOG" id="ENOG502QQMM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045854_2_0_1"/>
    <dbReference type="InParanoid" id="G3AG72"/>
    <dbReference type="OMA" id="FAYEDED"/>
    <dbReference type="OrthoDB" id="207120at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057402">
        <property type="entry name" value="AIM3_BBC1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25459">
        <property type="entry name" value="AIM3_BBC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BBC1/AIM3 cysteine proteinase-fold" evidence="2">
        <location>
            <begin position="357"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="503" mass="54114" checksum="79A0096EFD10F19B" modified="2011-11-16" version="1" fragment="single">METEGTGYDADIDLSDISKPNEATIVRPPSPAKSIDATDVDAEELESRETLEDKTQPDSVPPFPTTSVPPPVPVSERPPPPPVPIPVPERALPPPVPSSERPPPPPVPVAERAAPPPIPVGIPPIPAAAPPVPGAAPPVPGAAPPVPGAAPPIPGAAPPVPPTGDFAYEDEDSSSSDEEGNASLTEVAHPHDQPLRSQTLGVPPVPPPSNIPPIIPPVPRRANTEAIPQLAKAPSGLDARSSLDSSRNRALKGSNTDLGQTQAEACHQQIENELANLQSSSNWWVRGELPDCLSSKIGSELIFEIDSNTVAKRGKRTIEYKDYYILFYDLSQLVFELAFETQDPKGTILLVNYFVKPVPIIRKDLLDRYHKQFGGAIVSRASQFLGTKINDDLVNQVFDDVNANKSLIPVIGNKSYGVTVYKNFNNSNVSKLDDIRAGDILWVKNGKFTTHKGIMGGKSITLGDGPENIYSAIIYEYDSKKDKFKVFERDGSGQIKKENYKIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGH7</accession>
    <name>G3AGH7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ERCC1-like central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_58541</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERCC1/RAD10/SWI10 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372728.1">
        <property type="nucleotide sequence ID" value="XM_007372666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGH7"/>
    <dbReference type="FunCoup" id="G3AGH7">
        <property type="interactions" value="132"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGH7"/>
    <dbReference type="GeneID" id="18872497"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58541"/>
    <dbReference type="eggNOG" id="KOG2841">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041616_3_0_1"/>
    <dbReference type="InParanoid" id="G3AGH7"/>
    <dbReference type="OMA" id="HPEYIWQ"/>
    <dbReference type="OrthoDB" id="10262814at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070522">
        <property type="term" value="C:ERCC4-ERCC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22325">
        <property type="entry name" value="ERCC1_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000001">
        <property type="entry name" value="DNA excision repair protein ERCC-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047260">
        <property type="entry name" value="ERCC1-like_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004579">
        <property type="entry name" value="ERCC1/RAD10/SWI10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00597">
        <property type="entry name" value="rad10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12749:SF0">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12749">
        <property type="entry name" value="EXCISION REPAIR CROSS-COMPLEMENTING 1 ERCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03834">
        <property type="entry name" value="Rad10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC1-like central" evidence="8">
        <location>
            <begin position="119"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="34"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="57"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="342" mass="37959" checksum="B0EB9270542A6AFF" modified="2011-11-16" version="1">MSEPNDNENNPSEDIDATSFASILAGVKRMREQYGGASVTEAVETVPEQASVSEPQPVSIPEPSSITTPRTSPIPSTSSRTTLLKPTAEPLRPYQQPAPVNSTSTSKHSTHRSASIAEILVSKSQTGNPLLKESMMKLTPWKFDSTILSDYYISPTFQILFLSLKYYKIKPEYIWTRLKKWNKGTSVVASQRINDRALRILLVVVDIDGHQEVLRKLSDICIKNDLTMVLAWSFEEAGNYIAMAKQLDNAPSKTISSIKGIRGVDYNSSVVEAFTGIRSVNKTDVSNLLANCKSVKQIVLESCKEDSENMLADIGGLGSTKLRNLRQVFSEPFIYNKQYNQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGK9</accession>
    <name>G3AGK9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Kontroller of growth</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_69614</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat RAPTOR family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372760.1">
        <property type="nucleotide sequence ID" value="XM_007372698.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AGK9">
        <property type="interactions" value="833"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGK9"/>
    <dbReference type="GeneID" id="18875280"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_69614"/>
    <dbReference type="eggNOG" id="KOG1517">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001136_0_2_1"/>
    <dbReference type="InParanoid" id="G3AGK9"/>
    <dbReference type="OMA" id="TEVCTND"/>
    <dbReference type="OrthoDB" id="10262360at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030874">
        <property type="term" value="C:nucleolar chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030307">
        <property type="term" value="P:positive regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031139">
        <property type="term" value="P:positive regulation of conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000947">
        <property type="entry name" value="Ubiquitin-binding TORC1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001464">
        <property type="entry name" value="Ubiquitin-binding TORC1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004083">
        <property type="entry name" value="Raptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029347">
        <property type="entry name" value="Raptor_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848:SF16">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14538">
        <property type="entry name" value="Raptor_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01547">
        <property type="entry name" value="YEAST176DUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM01302">
        <property type="entry name" value="Raptor_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Raptor N-terminal CASPase-like" evidence="6">
        <location>
            <begin position="83"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="1325"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="430"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1285"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1285"/>
            <end position="1295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1437" mass="163333" checksum="00374EEDFB4B96B1" modified="2011-11-16" version="1">MSNQPVYFGPRPPQQLNDKMRHGFDEDYNSENFLNLLANTFYIYFDDKRQNTNGNPITQEMKRTSKYYKNYQPISDWKVMKERQKTISAVLVLCLNLGVDPPDIMKTYPCAKYEAWCDPSTFTDTKKAIENIGKNLQSQYETLSLRTRYKQSLDPCVEDVKRFCNTLRRNAKDERILFHYNGHGVPQPTASGEIWVFNRGYTQYIPISLYDLQTWLGAPVLFVYDCNSAGNIVHNFKKFVQKRIDDDNDGNHDTSAPSPTSAYLDCIQLAACKSNELLPMTPDLPADLFTCCLTCPIDISIKWFIMQSPLKKNYYDLLPKDSIGNVIIPGKLTDRRTPLGELNWIFTAITDTIAWTSLSRPIFKRLFRQDLMVAALFRNFLLAKRIMPHLNCNPISDPPLPEAVRFHTMWNSWDLAIDQVLSQLLKNQEGGSSVNPVTSRQPSIDTTPANTSLGSTHLNGTAAPTGQGQPQQNLGNYQHSTFFEQQLTAFEIWLKYAGPSTKEPPEQLPIVLQVLLSQVHRLRALILLSKFLDLGPWAVYLSLSIGIFPYVLKLLQSPAQELKPVLIFIWARIMAIDYKSTQQELCKDKGYNYFYLILNSNPLNQIGAPGAPGSFSGSASGTMLINDDHKAMSAFILTLFIKDFKNGQRLCFSVEIINNCLKFIQNSENPLLRQWCGLLLSQLWNRYPDGKWIVYKDGYINRLLTLINDPIPEVRTSIIVAITTYLSDPLHNQQQQRQQQQASNGSDLRKDEIRQQDLKLANAILSLLGDGSSMVRKELIFFINKFIHTYSKFFLVVAFNQLEEEIVLIDNPNSLNEFRKRSPAYGSIFSSIWKALLILSEDPHCEVKQLAEILIDSVMVKLNKSELASVVKEMQDYLLSKSTINISESFKPIKPIMNRPLGDRRQVSSGATVTPRKNNLLIDGTSLTTRAASTSKLEHDHDNSDASSISSKLKGLSVSKLFKTFQINDDSEIKSFTRILNSGPVQTTYGNEYKPKTPMFKPRDLSENPQLPHESGFFEYSCEYFQEPQMSKNEIDEPGSKDYVKRLWRRNRNESIIQMTQPQKEMAVRGDWNKNISVLNNKSQPKCIKFTQFEKILVASDEKDNISVWDWEQNQIVKKFSNGNPFGTKITDIKFLNEDDLPLLLTGSSDGVIKIYKNFHNYERFGYGQASDDLEADEDKKVELIAGWRALTDLLLTSKSSGLISEWQQSRGSLLVSGDVKIIRIWDAPRELCLVDIPARSSSSITSLTSDQVAGNIFIAGFDDGSLRVYDRRLDSRESMVKTWRATSASGGSTPQQQQQQQQSRYTGAGSIRNVHMQRGGFRELVSASADGYVNLWDIRLNDPVMSYSTPDRSLRCMDVHEHAPIITTGSKSVNIWSTSGDLLTTLRNPHDKYLSNRTSSYLSNTIFHPHRMLVATNYNQDGHINVYSCSDTIVEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGL2</accession>
    <name>G3AGL2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MICOS complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58578</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372763.1">
        <property type="nucleotide sequence ID" value="XM_007372701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGL2"/>
    <dbReference type="FunCoup" id="G3AGL2">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGL2"/>
    <dbReference type="GeneID" id="18872512"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58578"/>
    <dbReference type="eggNOG" id="ENOG502S70K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086433_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGL2"/>
    <dbReference type="OMA" id="QMIFNES"/>
    <dbReference type="OrthoDB" id="2399148at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044284">
        <property type="term" value="C:mitochondrial crista junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019166">
        <property type="entry name" value="MIC26/MIC27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033181">
        <property type="entry name" value="Mic26_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28268">
        <property type="entry name" value="MICOS SUBUNIT MIC26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28268:SF1">
        <property type="entry name" value="MICOS SUBUNIT MIC26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09769">
        <property type="entry name" value="ApoO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="247" mass="27010" checksum="562C94E8C13DBDDF" modified="2011-11-16" version="1">MFGRAFRFGIPVLSAGAVIATSNNGIVHNDSKKRNFYEDERDVVNIPGTVTPAPASEIAALGSNRIIDGISVRSTGTTEQIFKSVREFTESTADSIQSWLNSSYTKYNATERAVADTVSGLHNRSEDLLPNSIYIVIAFLAGTIAARPRGVLARVTFPTIFGLGAFKYFLPRTFDNSMQFLWQVEQNKVPALAKQQEATYNSAVGLVHSIEETTENSKKSIEARTAALRKSIADITGLNIDEEVSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGY2</accession>
    <name>G3AGY2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1690-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_53096</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372068.1">
        <property type="nucleotide sequence ID" value="XM_007372006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGY2"/>
    <dbReference type="FunCoup" id="G3AGY2">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGY2"/>
    <dbReference type="GeneID" id="18871758"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53096"/>
    <dbReference type="eggNOG" id="ENOG502SDJV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093897_2_0_1"/>
    <dbReference type="InParanoid" id="G3AGY2"/>
    <dbReference type="OMA" id="GKSLNCW"/>
    <dbReference type="OrthoDB" id="333239at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044284">
        <property type="term" value="C:mitochondrial crista junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012471">
        <property type="entry name" value="DUF1690"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07956">
        <property type="entry name" value="DUF1690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="151" mass="16634" checksum="49EA5A09842F656B" modified="2011-11-16" version="1">MGSSASKPETKVFTPATPIDFSASFLSQLENTPESEYSRAQHTEKYIQERVAAELSKLESQTIAQFQNTTNSALLTDDDKTTALPSVAALNEKIAKLTQVLQENAKLAKIEVPSTIAASREAVISCLKENQGKSLNCWEEVEVFRKLVKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH03</accession>
    <name>G3AH03_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal RNA-processing protein 41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_58653</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Component of the RNA exosome complex. Specifically part of the catalytically inactive RNA exosome core complex (Exo-9) which may associate with the catalytic subunits RRP6 and DIS3 in cytoplasmic- and nuclear-specific RNA exosome complex forms. Exo-9 is formed by a hexameric base ring of RNase PH domain-containing subunits and a cap ring consisting of CSL4, RRP4 and RRP40.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372845.1">
        <property type="nucleotide sequence ID" value="XM_007372783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH03"/>
    <dbReference type="FunCoup" id="G3AH03">
        <property type="interactions" value="884"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AH03"/>
    <dbReference type="GeneID" id="18872555"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58653"/>
    <dbReference type="eggNOG" id="KOG1068">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063514_0_1_1"/>
    <dbReference type="InParanoid" id="G3AH03"/>
    <dbReference type="OMA" id="ECRINTH"/>
    <dbReference type="OrthoDB" id="437922at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071031">
        <property type="term" value="P:nuclear mRNA surveillance of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070478">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030847">
        <property type="term" value="P:termination of RNA polymerase II transcription, exosome-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11370">
        <property type="entry name" value="RNase_PH_RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000004">
        <property type="entry name" value="Exosome complex component Rrp41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF0">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0271">Exosome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="8">
        <location>
            <begin position="18"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="9">
        <location>
            <begin position="152"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00063066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="242" mass="26834" checksum="91A4926C12FB0B26" modified="2011-11-16" version="1">MELYSPEGLRIDGRRWNELRRFECKINTHPHSADGSSYVEQGNTKIICMVRGPMEPAMKSQVNATSSTVEVNVSLANFSTFERKKRSKTDKRITELKTTLERTFNECIMGHLYPRTLISVTVQVLAQDGGLLAAMTNAVTLALIDAGIAMYDYVSGVNAGLYDQTSALLDLNTLEENDMCSLTIGVIGKSEKLALVVLEDKLPLDRLESVLAIAIAGSHRIRELMDMEVRKHGNMRLAKVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH86</accession>
    <name>G3AH86_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_146854</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372928.1">
        <property type="nucleotide sequence ID" value="XM_007372866.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH86"/>
    <dbReference type="FunCoup" id="G3AH86">
        <property type="interactions" value="394"/>
    </dbReference>
    <dbReference type="GeneID" id="18870728"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_146854"/>
    <dbReference type="eggNOG" id="KOG0039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010365_4_0_1"/>
    <dbReference type="InParanoid" id="G3AH86"/>
    <dbReference type="OMA" id="DEVCCRD"/>
    <dbReference type="OrthoDB" id="167398at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000293">
        <property type="term" value="F:ferric-chelate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF9">
        <property type="entry name" value="FERRIC REDUCTASE TRANSMEMBRANE COMPONENT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442321" description="FAD-binding FR-type domain-containing protein" evidence="14">
        <location>
            <begin position="17"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="369"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="15">
        <location>
            <begin position="403"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW35516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="699" mass="80091" checksum="F7E0502FDBDBC15E" modified="2011-11-16" version="1">MKILCLVFIFAIGALAHGSGLEFYKQAAPLYGCNYIISYTGYHFCPPTDKTCLCTNENSVATIAGCLANTNSNTTYVVDHFVPEYCHEYYLTELEPDWYNKALEYFNKNARNASEVEATETPIDFPIRFEQAELERYQATSINYFNNRDDSIWYGVALFGFWLVVLIIAAVSHWSKILFPGFIKKMTGPISNFWRKYISAPALLGKRKAQFMPGFKVLDSLIPSRYESIVVALFYIVTIITQSINLKAIENDPVYGTKYAAEMRYVGDRTGIVGTIIMPLVMLFAGRNNFLQWLCGINYATFLCYHRHIARVMFMLVVVHSVTYSIFEEEYYYSDFKQPWFYWGVIATTIGGFMLIQSILYLRRYWYEMFLFIHIVFAAVYIAGTWIHIVNLGYGIILYPSVAVWCFDRLVRLCRLTYFGFPESTLTLVGNETIKVTIPKPRYWSPIPGGHAFIHFIKPTYFWQSHPFTFTSSAEENNIVMYIKLKGGVTHSLYKLLNKSPGKTMIMRVGVEGPYGEATPAKYSDKAVFIAGGNGIPGIYSEIYDIGLRAPKASKQVLKLIWVIRDYASISWFHEELKTLQNTNIDITIYVTRPSNFSKIEESNSSKEGSIKSSELVNSYESVDVIEQLKELSHIHFKEGRPNLEQLVQDEVKDSPGSIAFVVCGHPIMVDEVRYQCVKNIGNPEKKRVDFYEQLQVWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHC5</accession>
    <name>G3AHC5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_59511</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the neutral sphingomyelinase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373673.1">
        <property type="nucleotide sequence ID" value="XM_007373611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHC5"/>
    <dbReference type="FunCoup" id="G3AHC5">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHC5"/>
    <dbReference type="GeneID" id="18872949"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59511"/>
    <dbReference type="eggNOG" id="KOG3873">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034001_5_0_1"/>
    <dbReference type="InParanoid" id="G3AHC5"/>
    <dbReference type="OMA" id="IEESSMF"/>
    <dbReference type="OrthoDB" id="387657at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052714">
        <property type="term" value="F:mannosyl-inositol phosphorylceramide phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004767">
        <property type="term" value="F:sphingomyelin phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072711">
        <property type="term" value="P:cellular response to hydroxyurea"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090266">
        <property type="term" value="P:regulation of mitotic cell cycle spindle assembly checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030149">
        <property type="term" value="P:sphingolipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000073">
        <property type="entry name" value="Inositol phosphosphingolipid phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038772">
        <property type="entry name" value="Sph/SMPD2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320:SF24">
        <property type="entry name" value="PHOSPHODIESTERASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320">
        <property type="entry name" value="SPHINGOMYELINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="384"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="14">
        <location>
            <begin position="21"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW34089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="435" mass="49343" checksum="6A994E3443F91056" modified="2011-11-16" version="1">MSTTTLTNEKTTPSSPTVKLLTFNTWGLKYISRHRTERMQAIANKLANPTSPDDDYDIVALQEIWCEDDWECIQQACKDRYPYRRAFKSGIVSGPGLAVLSKIPIAETFLYRFPINGRPSAFFRGDWFVGKGVAVTLFKSHDGKALPIALLNSHMHAPYATNGDASYSTHRACQAWDFTKLVKMLKQARYAVIQVGDLNSKPGSLPYKLFTIEGGLSDSWNLVHEQRYEDNLIELSPHDQVLHAGITCNSRLNSYRQKSEPWQACRLDYALIDSNNITPIKASVKFTERLPAPLDCSYSDHFAYSTEFTINSKDEFAPASYEDQQITLDAKESIYRELLAEVSEYRQFTIPFQATWRKVHFFLSLIITIALHVGVYFVSSVAGWIPVIFLLVTVVVILSGTINGLIWYCGVRPESRALQEVQMEVEDAYTCLKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHD8</accession>
    <name>G3AHD8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59523</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373686.1">
        <property type="nucleotide sequence ID" value="XM_007373624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHD8"/>
    <dbReference type="STRING" id="619300.G3AHD8"/>
    <dbReference type="GeneID" id="18872957"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59523"/>
    <dbReference type="HOGENOM" id="CLU_1536207_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHD8"/>
    <dbReference type="OrthoDB" id="365981at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="175" mass="20079" checksum="87D2BF08266C36DC" modified="2011-11-16" version="1" fragment="single">MNGTESNDELEEQEVILGNSRKYKEKHRNDVRLNYDSDSSEEDNVEEELEDNQQSKQVQEDDMFASGEEDVANGEHDEEDDMFASDDESAVKQVQFQDSANRVYALGLTDEEEEEEDDDDEQDSETQSAPKIEAFNLDEESNEGHFDKEGNYVANKEEAITGDDLWLNDFKKSDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHG3</accession>
    <name>G3AHG3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_65297</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373711.1">
        <property type="nucleotide sequence ID" value="XM_007373649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHG3"/>
    <dbReference type="STRING" id="619300.G3AHG3"/>
    <dbReference type="GeneID" id="18875033"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65297"/>
    <dbReference type="eggNOG" id="KOG1216">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_474994_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHG3"/>
    <dbReference type="OMA" id="YETFHIS"/>
    <dbReference type="OrthoDB" id="4026618at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442088" description="Hyphally-regulated cell wall protein N-terminal domain-containing protein" evidence="7">
        <location>
            <begin position="20"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="8">
        <location>
            <begin position="11"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="302"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="302"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="352"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="406"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="433"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="462"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="520" mass="55121" checksum="C5B3EDB06C8C43E2" modified="2011-11-16" version="1">MNWLLIFAAITLFFSDTIALDVTGTTTISGDAVAPEEDFSVEEGGELTLEPSLESVLYLGTLTVNQEGQLYFRELYAYETFHISTVVNNGFMLWDSSDVASVSNFYETSFENNGEAVILGQSLSLIMLLQSNTGSLTFWGTTGYFTLDSGVNTGNICFYNQKISWESPDGNGCVALFDSQLSVYYPVTSFVPTIVFQGTSSSLNVQTDVAGSSYKLVQFGSGNQFIITDISFSSFNYDPDTGILTITENGNPVVLDIGMGYDSSKFTVSSDGSSVSILYNDVVPPGANSGGCSCVLNTTPSWASSPSTSIENESTSTEEESTEVESDSSTEETTEAETTSSEYESSTEEVETSSTETSSTETSSTEISSTEESSSQSSTTESSSSVSTQTTSKSHHPQPTFCIHPINDKIKKFCDDYKNEHGHPNGNQPGNGNQPGNGNKPGNGNKPGNGNKPGNGNGNDNGKGNNQFPGSGKDNGNKPGFGNPNGNGNNKGNNQGHDNGNGNNNNNGKGGNNGHKRALH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHI0</accession>
    <name>G3AHI0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_59559</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373728.1">
        <property type="nucleotide sequence ID" value="XM_007373666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHI0"/>
    <dbReference type="FunCoup" id="G3AHI0">
        <property type="interactions" value="490"/>
    </dbReference>
    <dbReference type="GeneID" id="18872972"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59559"/>
    <dbReference type="eggNOG" id="ENOG502QQGC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010748_0_1_1"/>
    <dbReference type="InParanoid" id="G3AHI0"/>
    <dbReference type="OMA" id="RWMDSNV"/>
    <dbReference type="OrthoDB" id="65716at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071466">
        <property type="term" value="P:cellular response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045722">
        <property type="term" value="P:positive regulation of gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.10:FF:000002">
        <property type="entry name" value="Zn cluster transcription factor Rds2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056751">
        <property type="entry name" value="PAS_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053045">
        <property type="entry name" value="Zinc_cluster_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31986">
        <property type="entry name" value="REGULATOR OF DRUG SENSITIVITY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31986:SF7">
        <property type="entry name" value="REGULATOR OF DRUG SENSITIVITY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24990">
        <property type="entry name" value="PAS_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="62"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="264"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="265"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="309"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="539" mass="60762" checksum="B13D6A0BC97E8B6C" modified="2011-11-16" version="1">MVKTESPATAMSGSPGSTNTDNFGTPPKEGSDSQSQVDESTPSDSETTKQKPRRRKKKVEIACVYCRRSHMICDESRPCQRCIKRGIGHLCYDEPSNMRQRKKAATLRKTKSEGSSVPLQAVPPAPTMSIQSDIRNQNIPVQETQPQQLPLPHYRPQPKSLQKSVLSQTLPYNQEPFFYSEHAGSEFSSLNDFLSMIDDPELVHGALTDDPNAMLNFGVDQVQPQQQFPPGDGKTNSTTNLSSIFAMSPNANLFPATFGQPEILQQQSQPQPQQPQQLPAQQRQQTQPTQPSQPTQQPQQQSQQQSQQNFNQESHQPVISDSARDKFFLTAADPTTEISPEERLKQVIKAKLEAGLLQPYNYAKGYARLQSYMDNYMNITSRQRILKPLSIFRPAFRAIARTLKDVDLVLVEESFERMLLDYDRVFTSMAIPACLWRRTGEIYRGNKEFASLVGVTVDDLKDGKLAIYELMSEESAVNFWEKYGAIAFDKGQKAVLTSCNLRTKDGIKRKSCCFSFTIRRDRYNIPSCIVGNFIPIDPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHJ6</accession>
    <name>G3AHJ6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerases I, II, and III subunit RPABC3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_59580</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the eukaryotic RPB8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373743.1">
        <property type="nucleotide sequence ID" value="XM_007373681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHJ6"/>
    <dbReference type="FunCoup" id="G3AHJ6">
        <property type="interactions" value="824"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHJ6"/>
    <dbReference type="GeneID" id="18872977"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59580"/>
    <dbReference type="eggNOG" id="KOG3400">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103864_1_0_1"/>
    <dbReference type="InParanoid" id="G3AHJ6"/>
    <dbReference type="OMA" id="KEDDKGW"/>
    <dbReference type="OrthoDB" id="20018at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000191">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005570">
        <property type="entry name" value="RPABC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03870">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000779">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00658">
        <property type="entry name" value="RPOL8c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="148" mass="16682" checksum="F3D60903681471A5" modified="2011-11-16" version="1">MSGILFEDMFIVDSTDAARYNKVCRITGHSSTSQDIKITLDINSELFPVKTKDSLTITLASSLGNESSMLTSNGSWRPPKADDRSLADDYDYVMYGTVYKFEENADSDKMSVYISFGGLLMRLEGGYRSLSNLKQENAYILIRHYKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHM6</accession>
    <name>G3AHM6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">xylan 1,4-beta-xylosidase</fullName>
            <ecNumber evidence="14">3.2.1.37</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">1,4-beta-D-xylan xylohydrolase xlnD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Beta-xylosidase A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Beta-xylosidase xlnD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Xylobiase xlnD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">SPAPADRAFT_65353</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Xylan 1,4-beta-xylosidase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Hydrolysis of (1-&gt;4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini.</text>
            <dbReference type="EC" id="3.2.1.37"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycan degradation; xylan degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.37" evidence="14"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373774.1">
        <property type="nucleotide sequence ID" value="XM_007373712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHM6"/>
    <dbReference type="STRING" id="619300.G3AHM6"/>
    <dbReference type="GeneID" id="18875036"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65353"/>
    <dbReference type="eggNOG" id="ENOG502QR4D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004542_5_3_1"/>
    <dbReference type="InParanoid" id="G3AHM6"/>
    <dbReference type="OMA" id="GLPNYQV"/>
    <dbReference type="OrthoDB" id="47059at2759"/>
    <dbReference type="UniPathway" id="UPA00114"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046556">
        <property type="term" value="F:alpha-L-arabinofuranosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009044">
        <property type="term" value="F:xylan 1,4-beta-xylosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031222">
        <property type="term" value="P:arabinan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1700:FF:000007">
        <property type="entry name" value="Exo-1,4-beta-xylosidase xlnD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044993">
        <property type="entry name" value="BXL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42721:SF13">
        <property type="entry name" value="EXO-1,4-BETA-XYLOSIDASE XLND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42721">
        <property type="entry name" value="SUGAR HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0858">Xylan degradation</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442403" description="xylan 1,4-beta-xylosidase" evidence="19">
        <location>
            <begin position="19"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type III-like" evidence="20">
        <location>
            <begin position="696"/>
            <end position="766"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00026107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EGW34190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="788" mass="85910" checksum="98F85705C6ADF6AA" modified="2011-11-16" version="1">MLPIKLLATLALAIFANGETPPSSPDYNTEANPQLPPITFEAVHYTFPDCQNGPLKHNAVCNPHLPTEQRAKAVVDLFTVDELIANMGNTSPGVERLGLPPYQWWSEALHGIARSNFTASGEYSHATSFPQPILMGGAFNNDLYKQVGNVIGTEARAFNNVGRAGLDFYSPNINPFRDARWGRGQEVASESPVLVGNYALNYVQGLQGGLDSNQNDDTLQVAATCKHFVGYDMESWNQHSRLGYNAIISDQDLADFYLPTFQSCVRDAKAAGAMCSYNAVNGVPACASEFFLNTVLRDGFDFQNGVIHSDCDAIYNVWNPHLYAQDLGGAAADAIKAGVDVNCGDTYQNNLGYALGNKTINENQIRTSVTRQYSNLIRLGYFDSPQTNKYRKYDWNDVSTPQANQLAYQAAVEGIALLKNDGTLPFNKQKVRKVAVIGPWANATTQMLGDYAGTPPYMISPLQGAQSEGFQVEYALGTQINTTDTSGYTAALNAAKGADAIVYFGGIDNSVENEALDRESLAWPGNQLDLVSKLSGLKKPLVVLQFGGGQIDDTEIKNNKNVNAIVYAGYPGQSGGTAIWDILSGKYAPAGRLTTTQYPASYADQVPMTDMTLRPRQGYPGRTFMWYNGEPVYEFGYGLHYTTFSASLANAPRGGHQSFNIEQVVAAAKRSQYVDTGLITTFDVNIKNTGKTTSDYAALLYSKTTAGPGPHPNKILVSFDKLHQIHAGQTQTAKLPVTIGSLLQTDTNGNKWLYPGTYTFFVDNDKKAQWEITLTGQAELIQKYPSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHR1</accession>
    <name>G3AHR1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">TFIID subunit TAF5 NTD2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_134515</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat TAF5 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373809.1">
        <property type="nucleotide sequence ID" value="XM_007373747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHR1"/>
    <dbReference type="FunCoup" id="G3AHR1">
        <property type="interactions" value="877"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHR1"/>
    <dbReference type="GeneID" id="18869827"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134515"/>
    <dbReference type="eggNOG" id="KOG0263">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005884_0_2_1"/>
    <dbReference type="InParanoid" id="G3AHR1"/>
    <dbReference type="OMA" id="HNHPVWD"/>
    <dbReference type="OrthoDB" id="10266330at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08044">
        <property type="entry name" value="TAF5_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.500">
        <property type="entry name" value="TFIID subunit TAF5, NTD2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007582">
        <property type="entry name" value="TFIID_NTD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037264">
        <property type="entry name" value="TFIID_NTD2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF1">
        <property type="entry name" value="CANNONBALL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04494">
        <property type="entry name" value="TFIID_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160897">
        <property type="entry name" value="Taf5 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="TFIID subunit TAF5 NTD2" evidence="11">
        <location>
            <begin position="179"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="448"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="503"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="545"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="587"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="629"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="677"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="122"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="96"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="393"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW34225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="782" mass="87848" checksum="C24229BAFEFEDAA1" modified="2011-11-16" version="1">MAGEKTDQSDAQQVHLDQQSPVQQQARPTTNQPGGGSGGGNPTTGGTAPAQARPQQARGNQPQYSQADLNRIVLEYLNKKGYHRTESMLRLESTNTPTPSVAPVTPSTTALASPGELDGKKEKVLKDKLSRNERELRELREKQSKIERELRETKDREVRLAKEKDLQGLRELEQKKKRESDPAIYTTAYSMLRRWVDSSLDLYKPELSRVLYPLFIHCFLELISKGYDTDAKKFFDKFKSDHLILHGVEVSKFAGLALPEHLEENELAVAYRNHKYRILVSKTSLNLLLYFLHENEAVGGGILIRIINQYLDPVISINRPDKVDREGEANPEEGIPEYVTDTNEVEKFNEQGVKLGKLPIDPDVQKEIEAELKIKDEKIEPVNGKTLTEEFQEMTKPDQDSPARESLPLPLKDHGDIRRMILEVEDSRSKIKLGSIQASAPSVCMYTFHNTNNDMTCIDFNDDSNMVAAGFHDSYIKLWSLDGKPLKSVFKKDRHRHENTRKLIGHSGPVYGVSFSPDNKFLLSASEDKTVRLWSLDTYTALVSYKGHNQPIWDVKFSPLGHYFATASHDQTARLWATDHIYPLRIFAGHINDVDCVEFHPNSNYVFTGSSDKTCRMWDVQTGHCVRIFMGHTNPVNCIAVSPDGRWLASAGEDSVVNLWDIGTGRRLKTMKGHGRSSIYSLAFSRDGSVLVSGSADNSVRVWDVKRNTNDAGPEPEAFSLLGDGKTDNTTTANGKPSEKVNKKEIIATSDHMTAYFTKKTPVYKVHFTRRNLCLAGGAFSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHZ3</accession>
    <name>G3AHZ3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Phosphate metabolism transcription protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_148988</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373891.1">
        <property type="nucleotide sequence ID" value="XM_007373829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHZ3"/>
    <dbReference type="FunCoup" id="G3AHZ3">
        <property type="interactions" value="92"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHZ3"/>
    <dbReference type="GeneID" id="18870807"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_148988"/>
    <dbReference type="eggNOG" id="KOG1161">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4580">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009308_2_0_1"/>
    <dbReference type="InParanoid" id="G3AHZ3"/>
    <dbReference type="OMA" id="SFKFWVH"/>
    <dbReference type="OrthoDB" id="6493944at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033254">
        <property type="term" value="C:vacuolar transporter chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006799">
        <property type="term" value="P:polyphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14480">
        <property type="entry name" value="SPX_VTC2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.30">
        <property type="entry name" value="VTC, catalytic tunnel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018966">
        <property type="entry name" value="VTC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042267">
        <property type="entry name" value="VTC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF2">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 3 COMPLEX SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09359">
        <property type="entry name" value="VTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="717"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="758"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="8">
        <location>
            <begin position="27"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="599"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="614"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="809" mass="93874" checksum="C8DF784407E63AAD" modified="2011-11-16" version="1">MSVSPTTKEPINNSNTSTSSKEEHTKMLFGTKLEREIYQPWREHYIKYNQLKKLLKEGVILKNNWTDKDEQTFVSALDENLESVFGFQHEKFDELNDELNHLQSLTESPKDDFDVDSFSKKLDSILEQAQLLEHFQRLNYTGFIKIVKKHDRLHPNYSVKALLNVRLKKLPFHSEDYSPLLYKVGTLFQFLRDNYEVDQSLSKLSSFNDGALSGEFQSFKFWIHRDNLMEVKTTILRHLPVLIYKSGLGDNNDDDDDDDEDEEAEQGDNDQTINCLYFDNEHFDLYNHKLTKLNNSSTLRIKWIGKLSDKPKISLERKEFDVNSNFHMDEKIELRQKYINDFIINKNIPKKLVKLNGEEPVKTLLEFIKEHSLQPVLRTTYNRTAFQIPGDDKIRIIIDSNLTFIREDSFDPQLPIRDPQQWHRLDLDNAKNPQQFLRKGEFNKFPFSTMEIKIKKSSVKNFKKLQWINELINSSYLVKEIPNFSKFIHGVASLFLEDDKLDNIPMWFNELEADLINDDVPRIPTSASSSNQIASISDEDNLSKFKSMILKNNTSNFQPRSASFSGSMLYSTKEDDESIETPPPPAQITTIIEESSAKPVTPSYGLAGATTDDQSSDFEDEDESDLPKPSPLTRLLHIPSKLLNVDSEDEEIDLPVGVSKPNEWIKNMGPIKIEPKVWLANERTFNRWLHVTTLLSSLTFIIYSSTMASNFEGLSTFLAYFYFGLTLFSGVWGYYIFMKRRQIIMERSDKHLDNSIGPLIIAVGLIVALIINFIFGWKNLNLEDPEHQVFYMENPMHKKIHDFVVSLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI30</accession>
    <name>G3AI30_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_59753</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">GTPase involved in activation of the TORC1 signaling pathway, which promotes growth and represses autophagy in nutrient-rich conditions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the GSE complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the GTR/RAG GTP-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373928.1">
        <property type="nucleotide sequence ID" value="XM_007373866.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI30"/>
    <dbReference type="FunCoup" id="G3AI30">
        <property type="interactions" value="602"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI30"/>
    <dbReference type="GeneID" id="18873066"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59753"/>
    <dbReference type="eggNOG" id="KOG3887">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047421_2_0_1"/>
    <dbReference type="InParanoid" id="G3AI30"/>
    <dbReference type="OMA" id="NCRTFQE"/>
    <dbReference type="OrthoDB" id="26136at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071986">
        <property type="term" value="C:Ragulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016237">
        <property type="term" value="P:microautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903778">
        <property type="term" value="P:protein localization to vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11385">
        <property type="entry name" value="RagC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001086">
        <property type="entry name" value="GTP-binding protein GTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039400">
        <property type="entry name" value="RagC/D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF2">
        <property type="entry name" value="GH16429P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04670">
        <property type="entry name" value="Gtr1_RagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="346" mass="38980" checksum="AA490DD23A62236E" modified="2011-11-16" version="1">MEPTAKDNTNATILLMGLRRGGKSSICKVVFHNMQPLDTLYLESTSKPTTEQFSSLIDLSVMELPGQLNYFEPNYDSERLFSSIGALVYVIDSQDEYLNALTNLSMIIEFAYKVNPKINIEVLIHKIDGLSEDYRIDAQRDIMQRTGDELLDLGLEGVQVSFYLTSIFDHSIYEAFSRIVQKLIPELPSLENMLDNLVQHSSIDKVFLFDVNSKIYVATDSSPVDIQTYEVCAEFIDITIDLDDLYVENERTKGAVASGRDKELKSISHLSNGSILYLKQMIRGLALVALIRNDDVRKGSTDTNQDKSLAIIDYNVNLFKQSLIKIWKNSRLEQPTPNGGVADQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ71</accession>
    <name>G3AJ71_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peroxisomal membrane protein PEX13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Peroxin-13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_59186</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="13">Interacts (via SH3 domain) with PEX14 (via SH3-binding motif); forming the PEX13-PEX14 docking complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxin-13 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373412.1">
        <property type="nucleotide sequence ID" value="XM_007373350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ71"/>
    <dbReference type="FunCoup" id="G3AJ71">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ71"/>
    <dbReference type="GeneID" id="18872813"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59186"/>
    <dbReference type="eggNOG" id="KOG3875">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034386_2_0_1"/>
    <dbReference type="InParanoid" id="G3AJ71"/>
    <dbReference type="OMA" id="EGWFPKK"/>
    <dbReference type="OrthoDB" id="10037838at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11771">
        <property type="entry name" value="SH3_Pex13p_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000128">
        <property type="entry name" value="Peroxisomal membrane protein (Pex13)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007223">
        <property type="entry name" value="Peroxin-13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035463">
        <property type="entry name" value="Pex13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19332">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19332:SF1">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04088">
        <property type="entry name" value="Peroxin-13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="316"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00065871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW33828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="418" mass="44528" checksum="ABB86CD5256801B7" modified="2011-11-16" version="1">MSSSAPRTKPWEVASGTSAAAATGDQAIASSSSAAAVAPSATATSAGAVPIPDRPTSLTSDFTSIPETSAYGSNYGTMNNYGSRYGMGGYGSGMYGSSMYGSGYGGYGSGMYGSSMYGGGMYGSGMYGGYGGGMYGGAGGMYGQQPGGMNPLGEGTQATFQLIESIIGAVGGFAQMLEATYMATHSSFFTMVSLAEQFGNLKNALGSLLGIFALIKFVKKMLYKLMGKTYNNGLNIKEFGKFEKNQKKLEDSLKRQQQQSSSGKPKISFKPLLLFLAASIGFPYLLSKAIQKLNEQNQRRLQQQQNGPQGSPLDPANLQFAKTLYEFNPENPNIEIELKPNELVAILSKLDPVGNESKWWKVRSRSGKVGYVPSNYLSVIERRPELIQPAPAAQAPKETEIPGGFEKPEKLVEEFKGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJG1</accession>
    <name>G3AJG1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_59233</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373448.1">
        <property type="nucleotide sequence ID" value="XM_007373386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJG1"/>
    <dbReference type="FunCoup" id="G3AJG1">
        <property type="interactions" value="109"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJG1"/>
    <dbReference type="GeneID" id="18872829"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59233"/>
    <dbReference type="eggNOG" id="KOG1426">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005210_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJG1"/>
    <dbReference type="OMA" id="GWGNCRK"/>
    <dbReference type="OrthoDB" id="5370059at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF466">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="2"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="161"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="343" mass="37454" checksum="DDA1F1D55CEDF227" modified="2011-11-16" version="1">MFQILSCGSNGSCQLGIGEENDIDQNSLVPALFEGETSGQIAYKPKKIVCGGNHTMILFENGDLYSSGDNTYGQCGYTEQIKSGIFQKVPGQWLDAACGWEYSILINSNNQVFVCGKGLKGELGLGTSVTETHLTELFAVSGKHIIKVKSCMNHTILQCNDELWGWGSCRKGQLGTVGNVKVLYTPTKLQFEGLENCEIVDFCLGREFTIIQTKNKLLKFGKLILDSLPEVPGSEVGTMWSSIHLFKDRTINSFGNNSHGQLFPSDDSVPAERMSLGSEHGLILANNVVYTWGWGEHGNCGVQTKRPADDVTFDYLNVLYSGSHKVVLVGTGCATSWVVIEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKB8</accession>
    <name>G3AKB8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ethanolamine-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="10">2.7.7.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">CTP:phosphoethanolamine cytidylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_60920</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.14" evidence="10"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375092.1">
        <property type="nucleotide sequence ID" value="XM_007375030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKB8"/>
    <dbReference type="FunCoup" id="G3AKB8">
        <property type="interactions" value="572"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKB8"/>
    <dbReference type="GeneID" id="18873633"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60920"/>
    <dbReference type="eggNOG" id="KOG2803">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031246_2_2_1"/>
    <dbReference type="InParanoid" id="G3AKB8"/>
    <dbReference type="OMA" id="QCKYINA"/>
    <dbReference type="OrthoDB" id="40021at2759"/>
    <dbReference type="UniPathway" id="UPA00558">
        <property type="reaction ID" value="UER00742"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004306">
        <property type="term" value="F:ethanolamine-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044608">
        <property type="entry name" value="Ect1/PCYT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780:SF2">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="13">
        <location>
            <begin position="15"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Cytidyltransferase-like" evidence="13">
        <location>
            <begin position="214"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="356"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="356"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW33577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="379" mass="42799" checksum="9E1CD765AE776CEF" modified="2011-11-16" version="1">MYERPEGIENCRIWIDGCFDFAHHGHAGAILQARQLGRELYVGVHSDEEILHNKGPPVMRLDERLTAVSACKWSTKVIPSAPYVTSLDVMDEYGCKYVVHGDDITTDANGEDCYQICKDMGRFVVVKRTPNISTTDLVGRMLLLSKSHMFPKLDKTGSSVLFSEENLARFKQYATDNTGLAPKSAVYVKYNDHLDQIVAPSAEIEAKLKKIVYIDGGFDLFHPGHIELLKVVYQRAGEHNASVVLGLHEDVVINEYKGMNYPIMNLLERSLCVLQCRYVDAVVLGAPYKPTKAFLEELPGEVIKVCHGPTELETEGVYEDTSDLFLQIGPHKFDEMNTAFIVDRVLQNKAAFEERQKRKGWKAEKERELKAKEQQTNNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKI4</accession>
    <name>G3AKI4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_71412</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the importin beta family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375104.1">
        <property type="nucleotide sequence ID" value="XM_007375042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKI4"/>
    <dbReference type="FunCoup" id="G3AKI4">
        <property type="interactions" value="240"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKI4"/>
    <dbReference type="GeneID" id="18875379"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_71412"/>
    <dbReference type="eggNOG" id="KOG2022">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011832_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKI4"/>
    <dbReference type="OMA" id="WMLQYMN"/>
    <dbReference type="OrthoDB" id="2016913at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051345">
        <property type="entry name" value="Importin_beta-like_NTR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363:SF33">
        <property type="entry name" value="IMPORTIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363">
        <property type="entry name" value="TRANSPORTIN 3 AND IMPORTIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW33589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1015" mass="117283" checksum="022BBEE245850F27" modified="2011-11-16" version="1">MSSELDIRNVLEDIDTLYSSHDSSKIASVQDRLQTYQRSDEGYLLGTELLKNGSRNVKYFGALTLTVYFNNHDLPDVDSPFNAVIYNVLTLVHEGYDENLFIIRKLLSNLSLLYIKNFATFTQDPLYKLLAMMLGREQPVNDLIRGLQSKNEVELVLLFLSILVEDIIKQSTNLPELHNTIHSTLFEHYKLIYQYLSTIESLPYSTKLLSLDCITSLVVYIATAEGNSTQRYSHEDMDIFLLYVLKYLQDELNIENMEIYNKSFTVLAEFLENIPRLVNTFKQHISELLFNETKFGMKFVDLIFTNNEFAELYSSEIENFVNLVIAYLSINLVYISRNILNDSISNILKVVIKLSDFPGVPIEDEKVSEQLIPFWQDLADTFIDDADAISDIIFDKDTLDRFNNQKIAIFSEISQIYFKKAQITSASPAKEFFQYRTQIAELFILFYSLINTPIYSTLCDIVQSNLLIIKTNPEAMNDLETGLYLIFKITDDLRFYDDEFAMNTLVPLINGFFSQGLIESIESIPNYHNHQISVTLLNLLSCLHFFFKSDTGSTYLPSTFNFLFSMILSENTNPKLSLISSRTILKICQDSKDKLVSFLPNLELILIEMLKNPRFDNLIRERLTNSYISIAQSIKDPIDIGNKIYQILLQITEQYNVVVNTPNSLLTDIEIDQYDKYLEEYTTSLLSCVYEIGKAMVIPEELEDYLTPEQIGQYNAYWNEDPLSIKSMILTTLNQFSLQFDPLVQKTIITEKCCNILKCGLSEPINGPFKFNYSVIFEYLIAKIKKCNLQSIPYIYKLVETVVVTNKKELTSADIETILIPTFSDMYQVLESDVDLIKSSIEMFSTILETTPGLILTSQLFQDKVLNYALIGLKNYHETFIIKAVVKFWTNFVTLKRGSQQDQQIKNYLFIELQIDGIPLGYQLVSSLMVGFINAPRSALEYYYPLFRNLIGKFPMELKNWVLYFLENNSNNGKVELTNTEIQQFVSRLMVTRGQRVANDLLRDYWLKVNKLIDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKX5</accession>
    <name>G3AKX5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_60343</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374533.1">
        <property type="nucleotide sequence ID" value="XM_007374471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKX5"/>
    <dbReference type="STRING" id="619300.G3AKX5"/>
    <dbReference type="GeneID" id="18873353"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60343"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016304_7_1_1"/>
    <dbReference type="InParanoid" id="G3AKX5"/>
    <dbReference type="OMA" id="VRIFKMQ"/>
    <dbReference type="OrthoDB" id="446113at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050825">
        <property type="entry name" value="RBM42_RBP45_47-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640:SF10">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="89"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="190"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="309"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="64"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW33018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="457" mass="51755" checksum="A9C64AF239C4B25C" modified="2011-11-16" version="1">MSYNSNYRPRSSDHYNSRPRQGYQSSRPKNTYNNRYQDQSQHHHSSSPSSSSSSSSYTQRKRTGTYNSYQSRQNVYSQHSSRYTQDNQFQLWMGDLDSNWTEEAIDYIWASLVEKPVSVKIIRDKLNPTKPGYCFVTFNNQKSVDLAMQRNGQPVPSSNKYFKLNYASGGGHGGGQSRHAASGGDSSNDFSMFVGDLGHEVSEALLFNKFNHKYPNQIKHVKVIIDPTTKKSKGFGFVRFLNGEALNRALQEMNGVEIGSKAIRVGLASGASVDIQKGPTSSQGTVDYRRVVVPQPQPDLNQYTDYDNTSLVIKGLASKFTERELEMYFIGFGDLIHCKLSSDFQTGYIKYYLRSSAESAILYMHGSTINDCRLTINWGKPDESETTTSYEKSSKPPLIFYSNEYNNIRLDKLDKEQVSTAIAQRLDGSEIKSINQTNQLYIQSKLNRDKILNSSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL70</accession>
    <name>G3AL70_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ADF-H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_60426</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Interacts with G-actin; ADP-actin form.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374629.1">
        <property type="nucleotide sequence ID" value="XM_007374567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL70"/>
    <dbReference type="FunCoup" id="G3AL70">
        <property type="interactions" value="407"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL70"/>
    <dbReference type="GeneID" id="18873401"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60426"/>
    <dbReference type="eggNOG" id="KOG1747">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031995_0_2_1"/>
    <dbReference type="InParanoid" id="G3AL70"/>
    <dbReference type="OMA" id="YLFKHTH"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11285">
        <property type="entry name" value="ADF_Twf-N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028458">
        <property type="entry name" value="Twinfilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13759">
        <property type="entry name" value="TWINFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13759:SF1">
        <property type="entry name" value="TWINFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ADF-H" evidence="9">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="ADF-H" evidence="9">
        <location>
            <begin position="189"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="327"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="334"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="357" mass="39854" checksum="004A51A791B840AE" modified="2011-11-16" version="1">MSTQSGITASPELLDSFKNLKSQALIIKVSSDSTQLIPDPNYTSPTESSIESLFTSVHEHISKEFPHPSYIIISNEEDYVFISFIPDVAPIKQKMLYASTKNTLVSSLGSYNFPKNRTFAWTELDELSYSNYKKSVEDISTGPLSKDEEIIKTLNNLQDLNLSSQTSLDHHGFKKELPSIHDTASSSSSADILGKFTPELQQNFDSLKDNTSSFKLVTFNIQGDEIVLNRAETDVRLVDLIPSLTESENKEKPTPLYAVYNYHINKFAFIYSCPSGSPVRERMIYAASKVSLISHLNQILKPSNLSIDKNLEVGDLDELELSQLDIESEVPTPGSVSSTNSNRTGLKFNKPKGPRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALF9</accession>
    <name>G3ALF9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_60532</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL28 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374717.1">
        <property type="nucleotide sequence ID" value="XM_007374655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALF9"/>
    <dbReference type="FunCoup" id="G3ALF9">
        <property type="interactions" value="231"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALF9"/>
    <dbReference type="GeneID" id="18873453"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60532"/>
    <dbReference type="eggNOG" id="KOG3278">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090033_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALF9"/>
    <dbReference type="OMA" id="VQRDSYY"/>
    <dbReference type="OrthoDB" id="361870at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.40">
        <property type="entry name" value="Ribosomal protein L28/L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026569">
        <property type="entry name" value="Ribosomal_bL28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034704">
        <property type="entry name" value="Ribosomal_bL28/bL31-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037147">
        <property type="entry name" value="Ribosomal_bL28_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13528">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L28, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13528:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL28M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00830">
        <property type="entry name" value="Ribosomal_L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143800">
        <property type="entry name" value="L28p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="266" mass="30824" checksum="37447ADB9DB7E240" modified="2011-11-16" version="1">MNFLGGLRTPFSSGLNSVQSIINTRQLSSSLPSLSRHTPDKYWKITRYVKPINETVYEAGQEMPCGVKVPKERSAYPQYEYETLYFKRQNRGLYGGLQRRVTASRSEYLNKTLRVQLPNIRKTKLWSEALQKSIKTKASTKVLKTITREGGLDKYLTKEKPARVKTLGLKGWQLRYDVLKALEDKELPVVRKDDKTIRVKYIHPDGKQFIASKEELLAQLHAHVQNDSYYPIEWAKYQKENVHLSFGDIVEQLEGYNFDFSQVTAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALQ4</accession>
    <name>G3ALQ4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60635</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374813.1">
        <property type="nucleotide sequence ID" value="XM_007374751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALQ4"/>
    <dbReference type="GeneID" id="18873499"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60635"/>
    <dbReference type="HOGENOM" id="CLU_1006700_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALQ4"/>
    <dbReference type="OrthoDB" id="10020333at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="218"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="277" mass="29753" checksum="368F031AEC811FC4" modified="2011-11-16" version="1" fragment="single">MSSRQRSSSVTSISSNHSMYSSTTPSVLPPQPPPPPPILPSQPLSHGGGSAISTPTTMHSPIFPPHPIQGHNIAPTPNTARSMSIVSLESPRNSIISFDEVIRPHTRNNSSTSLVSLTSNAAGAATADTTTVAGTGTGMTTPITNEYVMSKRLKSGIVLLSDEESESEITHTPTRSIIRPIFKLRRKSRETSDQHSQFLQLKRDFKLKFDELISTTTQASSSPTSLLPTSPSTTVPHLIASPNIDANPIQLDSSLELPPPSHKKKSKPNTLIQQSML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALU5</accession>
    <name>G3ALU5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_149783</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF8 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374219.1">
        <property type="nucleotide sequence ID" value="XM_007374157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALU5"/>
    <dbReference type="FunCoup" id="G3ALU5">
        <property type="interactions" value="231"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALU5"/>
    <dbReference type="GeneID" id="18870841"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_149783"/>
    <dbReference type="eggNOG" id="KOG4336">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024798_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALU5"/>
    <dbReference type="OMA" id="NICISNF"/>
    <dbReference type="OrthoDB" id="2193813at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00076">
        <property type="entry name" value="HFD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08049">
        <property type="entry name" value="TAF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037818">
        <property type="entry name" value="TAF8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019473">
        <property type="entry name" value="TFIID_su8_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46469">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46469:SF1">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10406">
        <property type="entry name" value="TAF8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor TFIID subunit 8 C-terminal" evidence="9">
        <location>
            <begin position="174"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="367"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="118"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="231"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="393"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="416"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="433"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="478"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="488" mass="56476" checksum="5B0EA0500CA1C2B9" modified="2011-11-16" version="1">MSNIETSNNLQDESRVNIELDGVNVQEESSAMDFAFEKVIAIILQSKQFHCSRDFLDELTDLTIRFLNDLVGSLSEYTRLQRRQKPALSDMKLLFNLKGIDFNEIAEEISLCRNFKKSQSLVTEMKRVENQIIQAQSERNNTEVEENSPSLPFIINEQYEITNLVPRLNTKPSYVPGYLPDFPPDFTYQSTPKYMETITDLKELRLKLVSESRMIEKPLYNLIESDDKKWRAKFEEELSEFEREKQDKKVEGTHVPETKSFDFIAYAQKRKSIAEKQKQDLDNRIKARETNLFMKAELYYSPYSTIRPTPEIESYFKNVITDGFKDVITSVRKAEREKQERTQKLLEERTKRERELEAQRKANEISINFNRMDDSSSSSSEDEAESEKVLEFMFEEDKEPESEPNIVVIGGENKTEPPTETTETTNAISFADIEADDDESSDDDMEDMEALLEAEVAHEQQLPGSTDLPASALKSSDEDSDDDMEDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALW2</accession>
    <name>G3ALW2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Uroporphyrinogen decarboxylase</fullName>
            <ecNumber evidence="4 14">4.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_149827</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the sequential decarboxylation of four acetate groups of uroporphyrinogen-III (octacarboxyporphyrin) to yield coproporphyrinogen-III (tetracarboxyporphyrin) with the formation of intermediate hepta-, hexa- and penta-carboxylate porphyrinogens in the heme biosynthesis pathway. Acts on a number of porphyrinogens, but only coproporphyrinogen III can ultimately be converted to heme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>uroporphyrinogen I + 4 H(+) = coproporphyrinogen I + 4 CO2</text>
            <dbReference type="Rhea" id="RHEA:31239"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:62626"/>
            <dbReference type="ChEBI" id="CHEBI:62631"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>uroporphyrinogen III + 4 H(+) = coproporphyrinogen III + 4 CO2</text>
            <dbReference type="Rhea" id="RHEA:19865"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57309"/>
            <dbReference type="EC" id="4.1.1.37"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the uroporphyrinogen decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.37" evidence="4 14"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374236.1">
        <property type="nucleotide sequence ID" value="XM_007374174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALW2"/>
    <dbReference type="FunCoup" id="G3ALW2">
        <property type="interactions" value="937"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALW2"/>
    <dbReference type="GeneID" id="18870844"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_149827"/>
    <dbReference type="eggNOG" id="KOG2872">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040933_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALW2"/>
    <dbReference type="OMA" id="LWLMRQA"/>
    <dbReference type="OrthoDB" id="339900at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00321"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004853">
        <property type="term" value="F:uroporphyrinogen decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00717">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.210:FF:000004">
        <property type="entry name" value="Uroporphyrinogen decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00218">
        <property type="entry name" value="URO_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006361">
        <property type="entry name" value="Uroporphyrinogen_deCO2ase_HemE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000257">
        <property type="entry name" value="Uroporphyrinogen_deCOase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01464">
        <property type="entry name" value="hemE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21091">
        <property type="entry name" value="METHYLTETRAHYDROFOLATE:HOMOCYSTEINE METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21091:SF169">
        <property type="entry name" value="UROPORPHYRINOGEN DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01208">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00906">
        <property type="entry name" value="UROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00907">
        <property type="entry name" value="UROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 14" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="9 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 14" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uroporphyrinogen decarboxylase (URO-D)" evidence="16">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Uroporphyrinogen decarboxylase (URO-D)" evidence="17">
        <location>
            <begin position="147"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW32721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="362" mass="40986" checksum="A41A096C6379B96C" modified="2011-11-16" version="1">MPEFAPLKNDLILRAAKGEKVERPPIWIMRQAGRYLPEYHEVKGKRDFFETCRDPEIASEITIQPIDHFDGLIDAAIIFSDILVIPQAMGFEIDMVEGKGPVFASPLRSPEDLSRIDLNPDIKSKLDWAFKSITLTRTKLNGRVPLLGFVGAPWTLLVYMTEGQGSKMFRFAKEWIFKYPEAAHKLLQATCDACIEFLALQVVAGAQMLQIFESWAGELGPDDFNEFSLPYLKQIAEKLPKRLKELGVEEYIPLTVFAKGAWYALDDLCNAGYDTVSLDWLYRPEDAVKVVNGRRITLQGNIDPGVMYGSDEVIAKKVEKMIHGFGGGKQNYIINFGHGTHPFMKPERIEYFLKQCHKYGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALZ0</accession>
    <name>G3ALZ0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mitochondrial distribution and morphology protein 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Mitochondrial inheritance component MDM10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MDM10</name>
        <name evidence="7" type="ORF">SPAPADRAFT_60681</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the ERMES/MDM complex, which serves as a molecular tether to connect the endoplasmic reticulum and mitochondria. Components of this complex are involved in the control of mitochondrial shape and protein biogenesis and may function in phospholipid exchange. MDM10 is involved in the late assembly steps of the general translocase of the mitochondrial outer membrane (TOM complex). Functions in the TOM40-specific route of the assembly of outer membrane beta-barrel proteins, including the association of TOM40 with the receptor TOM22 and small TOM proteins. Can associate with the SAM(core) complex as well as the MDM12-MMM1 complex, both involved in late steps of the major beta-barrel assembly pathway, that is responsible for biogenesis of all outer membrane beta-barrel proteins. May act as a switch that shuttles between both complexes and channels precursor proteins into the TOM40-specific pathway. Plays a role in mitochondrial morphology and in the inheritance of mitochondria.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the ER-mitochondria encounter structure (ERMES) or MDM complex, composed of MMM1, MDM10, MDM12 and MDM34. Associates with the mitochondrial outer membrane sorting assembly machinery SAM(core) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Mitochondrion outer membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="6">The ERMES/MDM complex localizes to a few discrete foci (around 10 per single cell), that represent mitochondria-endoplasmic reticulum junctions. These foci are often found next to mtDNA nucleoids.</text>
    </comment>
    <comment type="domain">
        <text evidence="6">Lacks alpha-helical transmembrane segments, suggesting that it resides in the membrane via beta-sheet conformations similar to those predicted for other outer membrane proteins and porin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MDM10 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374858.1">
        <property type="nucleotide sequence ID" value="XM_007374796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALZ0"/>
    <dbReference type="FunCoup" id="G3ALZ0">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALZ0"/>
    <dbReference type="GeneID" id="18873521"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60681"/>
    <dbReference type="eggNOG" id="ENOG502QUN5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026505_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALZ0"/>
    <dbReference type="OMA" id="VPGYRQI"/>
    <dbReference type="OrthoDB" id="2103793at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001401">
        <property type="term" value="C:SAM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051654">
        <property type="term" value="P:establishment of mitochondrion localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070096">
        <property type="term" value="P:mitochondrial outer membrane translocase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03102">
        <property type="entry name" value="Mdm10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027539">
        <property type="entry name" value="Mdm10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28035">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28035:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12519">
        <property type="entry name" value="MDM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1 6" id="KW-1134">Transmembrane beta strand</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="458" mass="51404" checksum="E7F3F52625D7CDF1" modified="2011-11-16" version="1">MYTYMEYLQKCFYKSTNWNEDNIFSNITATSQALLEFPLPNGIKVDTSSKSTEYSASSFTLSNFHTINGSLAYLYSSIPLSNTMGTKEISLQDAIAGFRIIEPSVRNSIIKSKTAAATAKSSLLYGRMYFPGSALEAMIIKRITPYTQLLIKTISSPHLDKNGTMIIYLQNNTPKYSREFIYSTNESLLGFRCLYNFGASQNLSQALIPKFDNSVVSVGTEIWYAARTMSPGMSTAFRYSTRSTSTGKPLTMTLAINPIIGHISSTYTVKTSVASTFCSKYDFNLYSYASNLSLGFELYSYGKSNNFPTFKNHEVNMSSQENSYLRKHARNQRPKEVPLGYKHHNGVIINPIENQDNVFHINPTLLPNSPNSSEKFRNKTQIQHKNETVTAAFQNLVNESDFSSVLKFSTSLNDRMVKLLWEGRVKDFLVSTGVKVALNPTTNIPEFNKLGISFSYAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM24</accession>
    <name>G3AM24_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acetyl-coenzyme A synthetase</fullName>
            <ecNumber evidence="6">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ACS1</name>
        <name evidence="11" type="ORF">SPAPADRAFT_135964</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>acetate + ATP + CoA = acetyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23176"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="6"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374244.1">
        <property type="nucleotide sequence ID" value="XM_007374182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM24"/>
    <dbReference type="FunCoup" id="G3AM24">
        <property type="interactions" value="499"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM24"/>
    <dbReference type="GeneID" id="18869932"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135964"/>
    <dbReference type="eggNOG" id="KOG1175">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_1"/>
    <dbReference type="InParanoid" id="G3AM24"/>
    <dbReference type="OMA" id="AIKASWP"/>
    <dbReference type="OrthoDB" id="1706066at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016880">
        <property type="term" value="F:acid-ammonia (or amide) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019654">
        <property type="term" value="P:acetate fermentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019427">
        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05966">
        <property type="entry name" value="ACS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000004">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000001">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011904">
        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF14">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="10">
        <location>
            <begin position="40"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="8">
        <location>
            <begin position="104"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="9">
        <location>
            <begin position="553"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="676" mass="74826" checksum="950BFA625E471EDA" modified="2011-11-16" version="1">MVEQTQKSHISLDHEKMHAPPEGFFERSTSKPNLADFATYQKMYDQSIQDPATFFGEQAKQTLDWIRPFDQPRFPADKKDDFKNGDLPAWFVGGQINAAYNCVDRWAAKDPNKVAIIYEGDEPDSGRKITYGELLKDVCKLAQALTKLGVKKGDSVAVYLPMIPEAVVTLLAIVRIGAMHSVVFAGFSSTSLKDRILDADSRIVITADESKRGGKTIETKKIVDDALKDCPDVRNVIVFKRTGNSHVPFSPGRDLWWHDELAKYGNYFPPTPVDSEDPMFLLYTSGSTGKPKGVQHNTAGYLLGALLTTKYTFDVHEDDVLFTAGDIGWITGHTYCVYGPLLNGATSVVFEGTPAYPNFSRYWEIVDKYKVNQFYVAPTALRLLKRAGHKYVEPYDLSSLRVLGSVGEPIAAEVWHWYSENVGRNKAHVVDTYWQTESGSHLLCPLAGVTPTKPGSASLPFFGIEPKILDPTTGEELKGNDVEGVLAIKSAWPSITRGIFGDYNRYIDTYLRPYADHYFSGDGAARDLDGFYWILGRVDDVVNVSGHRLSTAEVEAALIEHDLVGESAVVGYADDLTGQAIAAYVSLKKHKLPADADLEAIKKELILTVRKEIGPFAAPKTVLFVDDLPKTRSGKIMRRILRKVLAGEEDSLGDISTLSNPQAVSQIIDVVKTSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM44</accession>
    <name>G3AM44_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_54773</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374264.1">
        <property type="nucleotide sequence ID" value="XM_007374202.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AM44">
        <property type="interactions" value="1427"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM44"/>
    <dbReference type="MEROPS" id="C19.099"/>
    <dbReference type="GeneID" id="18871883"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54773"/>
    <dbReference type="eggNOG" id="KOG1863">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003532_2_1_1"/>
    <dbReference type="InParanoid" id="G3AM44"/>
    <dbReference type="OMA" id="HTAHHRF"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010995">
        <property type="term" value="P:free ubiquitin chain depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03775">
        <property type="entry name" value="MATH_Ubp21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02659">
        <property type="entry name" value="peptidase_C19C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000128">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000016">
        <property type="entry name" value="Ubiquitin-specific protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024729">
        <property type="entry name" value="USP7_ICP0-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029346">
        <property type="entry name" value="USP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF644">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14533">
        <property type="entry name" value="USP7_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12436">
        <property type="entry name" value="USP7_ICP0_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="MATH" evidence="9">
        <location>
            <begin position="129"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="10">
        <location>
            <begin position="295"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1309" mass="151168" checksum="EFEFCC6C4D594070" modified="2011-11-16" version="1">MDVDINTKKQSIEEPGNADVDATRANGIDGTNRDISDLIEVIDITQQGIEDDVEIIEDDDDDEDEDDEDEGVIVERGVSDNDDDVIVIADDSDTENGNNKDPAAAKANDFAAIAAKTMKPVQDYPIKDETHYVWEIKDWNSLKEEKVRSPKFKCGGYEWNILLFPHGNQNNNSISIYMEPHPPLDEEGKPVDENWYVCAQFALDLWNPHHPEAHMCNGSHHRFNKGETDWGFSSLIELKQLTHGVNNLRNPTPHPILTNNQLNITGYVRIIDDSSTGVLWHNFIDYDSKLGSGYVGLNNQGATCYLNSLLQSYFTTKCFRKLVYEIPTHSTGELSNVALSLQRIFYLLSTSNEPVATLELTKSFGWDSSDAFTQHDVQELNRILMDKLETAMKGTKIEGRLNDIFVGKMKSYIKCVNVSYESSRVEDFWDIQLNVRGFQNLSESFKNYIEIEMLDGENKYQAGDQYGYQDAKKGVVFEAFPPVLHLQLKRFEYDFVVDDLVKIDDFYEFPDKIDLKPYLDEDLSDEIKNQNWNYKLHGVLVHQGSISNGHYYAMIKPYANTETWLRFDDDKVWKVTPTQVFQDNFGARDLTQTELSKMSRIEQQDHLMRRVTSAYMLVYYRESELPSILPDNDESIDESIPKHIPAQIKFEIEERERIEKQKQEALYYINVKLITINTMNHNAGFDLALDSTVVKFYDESLKGTLCDPKSFKVKKEDNFKVLNELARNELGYEDANGFRLITVCHRNNHTNRVDEPVRNELVDTNVNNVYFKSFNRKFDEMVFFVEELNKDIRAINKLTTITEKVTPESFTFDFVLNKIQDVGTQQESDLKFKNIEEYSKNILLFIKYFDPISQEVRALSHINVSKDDVIETIVEPIRELLGFNEDVSLELYEEISPIKIEKLDQGVSFDKQELSNGDIITVQVSNVNDLAEGKVYHNLKEYYRFLLTRIRISVEPFKAEIDEEDFDFVEGEGKEKEESVDIQIAKNLSKTFQIWASMDYSYHQLAAEIALRLNVDPNYLRIFVLSNKGQRYPLKTTNHLSQLFPKHIPANQLIHFEYEILNIPLKDYENLKAVKIHWLTSLLQYQVFEILISKTGTVHDLIEKLLHKVNIPKSHLKHLLCWAGNNHIFADLVRFDISLNQIQDGYDLYCGVFPVEVEILAAHSVVARFTDDEEVNEDDDVFDNETVKEEFILAKKEVKNLNLIPTFHFHKNTTYRHGVPFLLTVYRDEVFKNTKERLRKKLGLGIQAFEKLKIALVDNDETSSGSYVEDDELVLFDEIGKASNGVSIALDHPDRIKRSNQFDKGISIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM60</accession>
    <name>G3AM60_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_60114</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374280.1">
        <property type="nucleotide sequence ID" value="XM_007374218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM60"/>
    <dbReference type="GeneID" id="18873225"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60114"/>
    <dbReference type="eggNOG" id="ENOG502QPR4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024588_2_0_1"/>
    <dbReference type="InParanoid" id="G3AM60"/>
    <dbReference type="OMA" id="CLQAYPG"/>
    <dbReference type="OrthoDB" id="4676at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016813">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03884">
        <property type="entry name" value="M20_bAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010158">
        <property type="entry name" value="Amidase_Cbmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01879">
        <property type="entry name" value="hydantase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32494:SF5">
        <property type="entry name" value="ALLANTOATE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32494">
        <property type="entry name" value="ALLANTOATE DEIMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001235">
        <property type="entry name" value="Amidase_carbamoylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="3">
        <location>
            <begin position="231"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="437" mass="47942" checksum="F0645848B3AD4871" modified="2011-11-16" version="1">MTFSIDSLITQKGRLLATIHETAKWGAKGVWGTRETETGVCRLALSDLDKQVRDWFVKETKSLGCEVKIDQVGNIFAIFPGKNQGKPTGIGSHLDTQPNGGRYDGILGVLSGLEVLRTFKENNYVPNYPIALVNWTNEEGARFPKSMVASGVWAKQIPLQECLNLKSLDKVPVSFGAELKRIGYDGQTKASYLENPLAAHFEIHIEQGPILENEGKKIGVVTGVQAFEWNLVTVKGRSSHAGTTPMNTRSDAILTASRVIVMAVETALKHQGLATVGTLQLEPGSVNVIPNIVKFSLDVRHVEDEILATIMAEIKQKAEQIAQENVDSPHGKPLSIDFENITSSKAIKFNNTNIKTVKDAASQIFKQDEIREITSGAGHDSCFTSTRVPTSMIFIPSKNGISHSPEEYSSPEEVENGFQVLLRTIVLFDEMRALQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMM6</accession>
    <name>G3AMM6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nucleoporin NSP1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_60820</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleoporin NSP1/NUP62 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374985.1">
        <property type="nucleotide sequence ID" value="XM_007374923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMM6"/>
    <dbReference type="STRING" id="619300.G3AMM6"/>
    <dbReference type="GeneID" id="18873588"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60820"/>
    <dbReference type="eggNOG" id="KOG2196">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4719">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_734812_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMM6"/>
    <dbReference type="OMA" id="DIFRAPY"/>
    <dbReference type="OrthoDB" id="344345at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026010">
        <property type="entry name" value="NSP1/NUP62"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007758">
        <property type="entry name" value="Nucleoporin_NSP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12084:SF0">
        <property type="entry name" value="NUCLEAR PORE GLYCOPROTEIN P62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12084">
        <property type="entry name" value="NUCLEAR PORE GLYCOPROTEIN P62-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05064">
        <property type="entry name" value="Nsp1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin NSP1-like C-terminal" evidence="11">
        <location>
            <begin position="166"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="182"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="267"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="105"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW33470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="377" mass="39852" checksum="BA4493F7D1AA482A" modified="2011-11-16" version="1" fragment="single">LFGAKKDDDKPAATTGGFSFGAKKDDDKPAATTGGFSFGAKKDDDKPAATTGGFSFGAKKDDDKPASSGLFGAKKDEDKPAASGTTPSLTFGTKPDEKKDDSKPATAGSIAPTATTATTSVQPNLKPTKIEPIPVSIDNKTLDDLIIKWSKQLSTTSKIFNSYTEKDSLEAEATQSKIDQHLLFVENQQDELEKLLDNYEQQADILLNNIELNNNSAAANATAVMQSADAAAAAASTNGASAAAAAAAAAAAANSNNSSLSVTDKLREKAYHNAELLDERLDNLGENLSTLINEINSVSDVFNKNLINDLAEGNSGATTGSSDEKSLQKTSKGGENPVEEIVKLLNLHLENLKYIESTEEDLKKKLDKLSKTKKSHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMQ2</accession>
    <name>G3AMQ2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">DNA polymerase eta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_137729</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375011.1">
        <property type="nucleotide sequence ID" value="XM_007374949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMQ2"/>
    <dbReference type="FunCoup" id="G3AMQ2">
        <property type="interactions" value="601"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMQ2"/>
    <dbReference type="GeneID" id="18870069"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137729"/>
    <dbReference type="eggNOG" id="KOG2095">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012348_7_1_1"/>
    <dbReference type="InParanoid" id="G3AMQ2"/>
    <dbReference type="OMA" id="VCAQWQS"/>
    <dbReference type="OrthoDB" id="5723at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009314">
        <property type="term" value="P:response to radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.60:FF:000008">
        <property type="entry name" value="DNA polymerase eta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.100">
        <property type="entry name" value="DNA polymerase, Y-family, little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036775">
        <property type="entry name" value="DNA_pol_Y-fam_lit_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052230">
        <property type="entry name" value="DNA_polymerase_eta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873:SF1">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036603">
        <property type="entry name" value="DPol_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="Lesion bypass DNA polymerase (Y-family), little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UmuC" evidence="10">
        <location>
            <begin position="47"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="703" mass="79919" checksum="15C7017D2FF80CD4" modified="2011-11-16" version="1">MSVKPTKDASPPESDLLTAPKPSQFTYQDFWDLNDPAKAYLSPLSTIGLVDQNAFFAQVEQIRLGLSDQDPVVCAQWQSLIAVSYAARKFGIGRMDSIKSAKEKCPKLIVAHAAVYKRGESHWSYIDGLPDAANHKVSLDTYRRESRKIVRLIQQRFDLVEKASVDESYIDFGRKIYEKMLKLFPELSRQPGDPSEYLPTIPDKLPIEIQWEGVVIPSDSEIQRDDNAQEQIGKPKIEDWDDICLIVASKEMLNLRKVIHDELGYTTSAGLARNKLMAKLAGGFKKPDNQTIIRNCALNRFLTNFELTDITGMGGKLGENLMYRLEVPADKNSITYIRDNYTLEMLQEEIKEDPELALKIYQIVRGLRSTELTSWIELKSMTSTKNYLPQKPLQNLGEAYGWLKVFAGDLYNRLIDLDYENLDLSKSKLSKKEKGIIKRPKTLTIGIHSTTGIRQTMQMSIPCFRDLDRLKEIIEDGGYSLLRGFLESATNISAMNGQITMKALYEGDPYKVKILPLRNMSLSVSNFTTLSENALIESYATKNNTNTASSELKQINESIRSKRESTPPEVTAKNLSKSDKEHISKMFEAFQVEQSVSLHERERESTAKPKVNPDNIYKLFNDFHESTQNARLPDTAIKNNQKRKHNSIDILRKLQKKPKRSLLEELIESKFCPKCKIAVDDALDHNDFHIAMELSEKLNGSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMT5</accession>
    <name>G3AMT5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sorting nexin-4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Autophagy-related protein 24</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_60873</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375044.1">
        <property type="nucleotide sequence ID" value="XM_007374982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMT5"/>
    <dbReference type="FunCoup" id="G3AMT5">
        <property type="interactions" value="520"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMT5"/>
    <dbReference type="GeneID" id="18873612"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60873"/>
    <dbReference type="eggNOG" id="KOG2273">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027221_1_0_1"/>
    <dbReference type="InParanoid" id="G3AMT5"/>
    <dbReference type="OMA" id="HKKETNR"/>
    <dbReference type="OrthoDB" id="205639at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06863">
        <property type="entry name" value="PX_Atg24p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45949">
        <property type="entry name" value="SORTING NEXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45949:SF2">
        <property type="entry name" value="SORTING NEXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="12">
        <location>
            <begin position="149"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="522"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="66"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW33529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="610" mass="69336" checksum="FF804A8449C5701B" modified="2011-11-16" version="1">MSSEDQFTSIQWDRDEMDHTNNSHTDNTFSPIEEETEQQPSISGPLESTAEHSTSDLSGSRSSIKGIHEDKPEIKSEGSSDDPSNSLLISQEEHISNPTSTVTPPKSDENLTTPAHSIRKTSVPETEPVVTHEQIDVPAEEDLSFEKYTIKTTVTNPTRDLDTASKPFISYLVTTTTDHPSILKLSVEKKLKEGEQYLSISVRRRYGDFRYLYESLSNDYPTVMIPPLPSKSNFKYLTGDTFGTEFVNKRLHSLDRFIRFILQHRTLSQSPIFHLFITNSNDWATFTTSLRIKDINSEDSGFVNKVVNEDLITEKVMNFLTPSKHKRETNRDILEINDKLKKLYENLIKLDKIFVKLNRKNHELSVDYEQFSNQIIKLTSVQQKPAANGEIGEVNSSFKEETSIAANFKIFAESLSYFSTSWGKLYKYVDESFLVSLKDCSKYIVSLTNLIELQHNKKIDLQVLQEYLAKTRNELASLGGSVGAHPPPNPVINSYQTGGIVNNTAQLIKDTLSTSATPHIGSNATDNKVQKLENKVIQLENEIAMQTKLVNNLTNKIITEEYPNWDKFNKNELKHSMIGLCDEQINFYKGLIDKWGDAEMKLLKRLDELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMT9</accession>
    <name>G3AMT9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_71364</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375048.1">
        <property type="nucleotide sequence ID" value="XM_007374986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMT9"/>
    <dbReference type="FunCoup" id="G3AMT9">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMT9"/>
    <dbReference type="GeneID" id="18875376"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_71364"/>
    <dbReference type="eggNOG" id="KOG2273">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025790_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMT9"/>
    <dbReference type="OMA" id="DFKDPWG"/>
    <dbReference type="OrthoDB" id="289314at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06867">
        <property type="entry name" value="PX_SNX41_42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044106">
        <property type="entry name" value="PX_Snx41/Atg20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051079">
        <property type="entry name" value="Sorting_Nexin_Autophagy"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46979">
        <property type="entry name" value="SORTING NEXIN-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46979:SF2">
        <property type="entry name" value="SORTING NEXIN-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="9">
        <location>
            <begin position="82"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="501"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="505"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="610" mass="70554" checksum="9E1831AAB2E2CA9D" modified="2011-11-16" version="1">MSHENLFADIEQDNNPSFYGNPSIIQDPYTTHTNAQQLRNEQNSNGHPQSNGQVGGLNNDAFPNELVNSTLGISQKISELLDAEDLQIEIVNSERLVNSNVIVYTIQLSSKNSKIIVKRRYSEFKSLRDNLLRLFPTLIIPPIPEKHSILSYLLNSINNANEINIIEMRRRYFKMFLDDLIFDSHENLKDCVLLHKFLDPNYELSWYNALNEPPVNRIPDNLLMANPVDPSDQNGLYSLLPIVNGFDFNSNIDNLPNLKKMNDDLLKLNDQVKLYELRGFEQDFEFLIPEELVMFEVKFHQIIKILSDLNKLDIRTSKNFKGLISILIDLGSNLNNFSLQIYHQKTNSNQLSESIEKFGSTIDQSFLNFESFITNHLIPEWQEPINQLVQYYYTALNLIKFYKYKIIQYKILYKLKFNKFQQLVNLSNLGVTPHTTNTESNSAPTSQGEIEVNIEHLQELNSPTLNNALKNLSKKKLTKKNSWYGLFGGNNQPKKFNFKLPEETQPQQQQQQQSAPPSSSSSSGSLKFKLNQIEKELNKLNQLIELCNNDMGILTKALVTTFEEFLVKLERKWLNLMINYIKNAKHLFIENLLNWQDFKESLESGSQEIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN21</accession>
    <name>G3AN21_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1774-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61504</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375712.1">
        <property type="nucleotide sequence ID" value="XM_007375650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN21"/>
    <dbReference type="STRING" id="619300.G3AN21"/>
    <dbReference type="GeneID" id="18873922"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61504"/>
    <dbReference type="eggNOG" id="ENOG502REZ6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061220_0_0_1"/>
    <dbReference type="InParanoid" id="G3AN21"/>
    <dbReference type="OMA" id="IWSILVY"/>
    <dbReference type="OrthoDB" id="3342455at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013920">
        <property type="entry name" value="DUF1774_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37992:SF1">
        <property type="entry name" value="DUF1774-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37992">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08611">
        <property type="entry name" value="DUF1774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="261" mass="30291" checksum="8F6F413F489A2AE8" modified="2011-11-16" version="1">MAHTANELKTYQGVTIVTLLFSIYGNLDYYYHSKHIATPFTASFWMLFAFWVVLYLKQILFTLFTFVQDDDRLRTILDLGWHFPVFNVLIYFWAELFIHKHYFWSEVVLIINLFNVSYLYTKHKSYSIKPITRFGLIHVSVAAMPFSWLLYAVFWNGALMFHVREKLWTRILANIFIWNFLGIGSLLVYAFDDCAVGFSLSYLMFALMVGQLTIKVFALQWIFALAIAVVLFLVSSGALVNRLRGESGQPSEAAPLLPEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANG3</accession>
    <name>G3ANG3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="23">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">SPAPADRAFT_152194</name>
    </gene>
    <organism evidence="28">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 23">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38567"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:38568"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphocholine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38583"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:38584"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(out) + ATP + H2O = a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66036"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:66037"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 22">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="23">Membrane</location>
            <topology evidence="23">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 23">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="23"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375228.1">
        <property type="nucleotide sequence ID" value="XM_007375166.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3ANG3">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANG3"/>
    <dbReference type="GeneID" id="18870918"/>
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        </source>
    </evidence>
    <sequence length="1655" mass="188319" checksum="A88E4D7009F0B311" modified="2011-11-16" version="1">MSQENNKSPFLDQEDSFSNDASSILPSPTMDRFAFNNPQNNIDGAFTDEEYDSVPPTPRIDNTRFEPINTNSNSRKGSLRFKLDDDNIPSSAHGNQKSGKIQSPFLDVHQVIPEDDIFKQPKSPFATEAFENSFDADEVEEDDDGEDDDAEESPSKTKRLRWGTRRNKHGRPKKEGVGRSLTLRRILDPHNKRKHGSHSLRLNSFRKPNTHSVDFPERHHHEESDDEDSNIDLEAEDPKNKKHQQRTIVFNRPLPADYVDPESGKTIKEYPRNKIRTTKYTPLSFLPKNISHQFFHNVANIYFLFLIILGAFSIFGVPSPVLAAVPLIVIVIITAIKDAIEDSRRTVTDLEVNNQTTHILSQINFDSEYHYDNINCNDSKVSAWRRFKKANTRLMVRIWGASKRNLTKEGRANKLRQKYNEEHNITEEPGRRSFDSDIIEASPRNSVDINPFSDDLRKSFQQQRRQSHKYQRSEKTLKFAKKYWKDVKVGDILRIYNNDEIPADVVILATSDDDNCCYVETKNLDGETNLKVKQAMKYSSIEQKITKADDLINHSFQVDSEGPHANLYTYQGNLKYDNNGAEDAQEAITINNLLLRGCSLRNTKWVIGIVVFTGDDTKIMLNAGITPTKQSRMSRELNYYVILNFIFLFIICFISGLVNGIYYTHHGTSRDYFEFGTIAGTPALNGLVGFFVALILYQSLVPISLYITIEIIKTAQAFFIYSDVGMYYPRLDFPCTPKSWSISDDLGQIEYIFSDKTGTLTQNVMEFKKCTINGVSYGRAYTEALMGLRKRLGVDVDTEAAVERELINKDKLQMIEKLHKISNNTTYDDEITFVSSKILDDMLGSSGDEQKNSVDHFMLCLALCHSVMSEQDPKNPKKLLLKAQSPDEAALVGTARSLGFEFKGNTKKGVLINVHGVTKEYQVLNTLEFNSTRKRMSSIIKIPGSTPNEPAKALLICKGADSIIYSRLSKTENDPELLETTSKHLEEFATEGLRTLCIAVRELSWEQYTEWNRRHQIAASSLEDRDDKMEVVADSIERELTLLGGTAIEDRLQDGVPDAISILAEAGIKLWVLTGDKVETAINIGFSCNLLGNEMELLVIKTGYSAEDTNRLGIRFPSGAGEQQVVDTIITHYLGHYFQMEGSLEEQEAAIGDHTPPDERFGVVVDGDALKMALLNPDTKRKFLLLCKKCRAVLCCRVSPAQKAAVVKLVKDTLDVMTLAIGDGSNDVAMIQAADVGVGIAGEEGRQAVMSSDYAIGQFRFLAKLLLTHGRWSYLRFSEMIPSFFYKNVIFNIALFWYGIYNNFDGTYLFEFTYLMFYNLAFTSLPVIFLGIFDQDVEAKVSLLVPQIYRSGILRTEMTQAKFWWYCIDGIYQSAISFFFPYLLYTIGFAGMNGKPVDHRFWMGVIVTCIACISCNLYILFHQFRWDWLSSLIVAISILIIFIWTGLWTINTYSGEFFKAAPQIFGTPGFWPTVFVGVLCCLIPRFFYDFVQRIFWPRDVDIIRECVQRGDFDAYPFDYDPTDPNRVKISAYTSDMINRMSFSSPRRSHQSKKVDNDSDEARNASDTVYEEEFSFSRRLATPPPTPPPGQSISPSKRRSFLRMFKKGSENSSNTYLPEMDSINPIQTEEIAMEDFSEHRREVSRISEENRGQHMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANL4</accession>
    <name>G3ANL4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Elongation of fatty acids protein</fullName>
            <ecNumber evidence="12">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_61610</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>an acyl-CoA + malonyl-CoA + H(+) = a 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:50252"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:50253"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ELO family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="12"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375820.1">
        <property type="nucleotide sequence ID" value="XM_007375758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANL4"/>
    <dbReference type="FunCoup" id="G3ANL4">
        <property type="interactions" value="626"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANL4"/>
    <dbReference type="GeneID" id="18873974"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61610"/>
    <dbReference type="eggNOG" id="KOG3071">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048483_6_1_1"/>
    <dbReference type="InParanoid" id="G3ANL4"/>
    <dbReference type="OMA" id="PISWVPI"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030457">
        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF134">
        <property type="entry name" value="ELONGATION OF FATTY ACIDS PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6 12" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW32544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="330" mass="37839" checksum="8CEE34632FC53CC4" modified="2011-11-16" version="1">MSAIPAPTWDTPFGVKLWPIFDTLVASLTHNSFVPSQFTFDTRLPLSTLPAVVTGITTYYVVIFGGDFIFKKFQIKPFVLNGLFQIHNLFLTSVSLLLLVLMVEQLVPMIYHHGLYYAICHPNAWSQELVCLYYLNYLCKFTEFIDTLFLVVKQKKLTFLHTYHHGATALLCYTQLIGLTPISWVPIVLNLGVHVVMYWYYFLAARGIRVWWKEWVTRFQIIQFVLDLGFVYFATYNKIVYTYFQQYLNVLPVCGDCAGTMLAAYSGCAILSSYLVLFIAFYIDVYKRKGSKKAKIVKSVKGGVAAQVNEYVYVTGRSDTPKPEGLKERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANR4</accession>
    <name>G3ANR4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal protein bL31m N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_50602</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375275.1">
        <property type="nucleotide sequence ID" value="XM_007375213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANR4"/>
    <dbReference type="FunCoup" id="G3ANR4">
        <property type="interactions" value="170"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANR4"/>
    <dbReference type="GeneID" id="18871458"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50602"/>
    <dbReference type="eggNOG" id="ENOG502RZ6E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130029_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANR4"/>
    <dbReference type="OMA" id="HEFECTV"/>
    <dbReference type="OrthoDB" id="5587740at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034600">
        <property type="entry name" value="Ribosomal_bL31m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048874">
        <property type="entry name" value="Ribosomal_bL31m_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28174">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L36, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28174:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL31M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21492">
        <property type="entry name" value="bL31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal protein bL31m N-terminal" evidence="2">
        <location>
            <begin position="34"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="177" mass="20497" checksum="9746B4B037DA5601" modified="2011-11-16" version="1">MFRQIVNPCVQHMSVRHYAGKAPETTGREVQLSSRRVMRKIRMGKARPAIFYQFDTLVELSDGSVIRRRSQAPKDEIRMLADQRNSSLWNPTRSDLIVDQSHTGKVSKFRTKYGDYDRGAGSEETNTTKSDLQTEEEREQARKLAAEREREFLEFMGEGAEIITHGKLYDKKADRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP35</accession>
    <name>G3AP35_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_152446</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375342.1">
        <property type="nucleotide sequence ID" value="XM_007375280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP35"/>
    <dbReference type="FunCoup" id="G3AP35">
        <property type="interactions" value="529"/>
    </dbReference>
    <dbReference type="GeneID" id="18870929"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_152446"/>
    <dbReference type="eggNOG" id="ENOG502QRPQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005934_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP35"/>
    <dbReference type="OMA" id="LSCNYCK"/>
    <dbReference type="OrthoDB" id="2943660at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF12">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="21"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="864"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="931"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="933"/>
            <end position="956"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1012" mass="115670" checksum="64850A39DA29049E" modified="2011-11-16" version="1">MQSSFQVAEKRKQKRNRISLSCISCKKRKVKCDRGNPCQACVKYHGVCEYPISVWTTASQNEPGSSYQIHGFDATKRRISEIESSLGHPSTGHSPFSTASNDSVLLPPIKKLHFNWNEYKYNPDTNPTYIGINPYANNTETINFYDGYTPLFLQDKARTHNYGPFSWMTIMNKDPGLRRLWKFVQNESLKKIEQHLTSTHRHKQIPHDEIVAEVAKQDANAAACFEISESEVAFRDKAVDRDGYNDLRLYGNVTRKTSNLRDSTASTSRSSNTPAPSQKQIQRMAMNKHAIALGLTLYEGKMDQELQLIEKITKILPKQKVIWKLIHKFFNSVYPYMPFLDEGYFVTEMTRILGPEESNDRQLESLKVEKRLDFAYIGILLIVLRITYLSLFSNRNGVNQRNLATTDPSFQAQEIKYLLSNPIDIDVIAMAQLCLDQFDIFHKSNIVILQCAFFMRLYYMFAPEEGDGADGGDSQIFSGTIIQMAYSMGLNREPDNFPDVSNDERSNNMARKIWLFLIIGDLIQGYQYGNPMSIDQKYYDTKLPYYKPGNENVKDIELEKHVISTFAYFEKYYSKLVALLDVGIDVRKHVTMKELTELITDFEISLNDHYGLLKFFLVPFKQDNYTYPFIKAMKCKNYINMKMFLNTILFHLYLHYEKSDTTFAFFYLKKILASLCTEFIPCMFQLIADNYLNFGPVADLFLNPTLESMIHKATVIISAVLVRVNSSIYKLRYVETDKHDDGLWNSPSYRLHYAKLVKFSKVLTRIMKFCIAAISRLSQRYYYAWRLTKAHGYIGTIIIGTEFYKNTKSDPILLIDLTNDQLSELLKISELTLKKYTKHVDIFNEDIDVINECAQSCSPPPEILGEEPDCSHNNAPPVKAQPTPDPHKQHLYNDSFASSSASLNDEDFGCLQNDEIDKLWYQMAAMKQTGPISTPSSNFGSSGSENGNYSNGNANNINQNPVTANVNMSYPVFGSPAGFNNIMGMGMGDGEVDLFNALSVDQLLGLTKDGKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3APG8</accession>
    <name>G3APG8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxamine 5'-phosphate oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61222</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375415.1">
        <property type="nucleotide sequence ID" value="XM_007375353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APG8"/>
    <dbReference type="FunCoup" id="G3APG8">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APG8"/>
    <dbReference type="GeneID" id="18873783"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61222"/>
    <dbReference type="eggNOG" id="ENOG502S4PT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078856_0_0_1"/>
    <dbReference type="InParanoid" id="G3APG8"/>
    <dbReference type="OMA" id="HPTIIMT"/>
    <dbReference type="OrthoDB" id="5300823at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052841">
        <property type="entry name" value="PMP_oxidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28040">
        <property type="entry name" value="PYRIDOXAMINE 5'-PHOSPHATE OXIDASE YLR456W HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28040:SF1">
        <property type="entry name" value="PYRIDOXAMINE 5'-PHOSPHATE OXIDASE YLR456W HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="PNPOx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase N-terminal" evidence="1">
        <location>
            <begin position="8"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="180" mass="20642" checksum="19603CFAE425BC80" modified="2011-11-16" version="1">METPDKLPDSVLSLLKSSRFIHLATCLNDKPHVSLMNYTFCQQGDKQIIIISTPTRTTKYQNMLSNPNVSILIHDWISVKDTTESKESVAQRRNSLFELLASFNKNEISRVSVMLDGKAKILDKNKDSETFNFYKSLHLNNPKIDENQVRNYIENENNALVLIEITGCKVTDTDNNIEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3APH0</accession>
    <name>G3APH0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_139002</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375417.1">
        <property type="nucleotide sequence ID" value="XM_007375355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APH0"/>
    <dbReference type="FunCoup" id="G3APH0">
        <property type="interactions" value="120"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APH0"/>
    <dbReference type="GeneID" id="18870169"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139002"/>
    <dbReference type="eggNOG" id="KOG3589">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024143_0_0_1"/>
    <dbReference type="InParanoid" id="G3APH0"/>
    <dbReference type="OMA" id="DPGMRYL"/>
    <dbReference type="OrthoDB" id="196547at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071444">
        <property type="term" value="P:cellular response to pheromone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000747">
        <property type="term" value="P:conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04450">
        <property type="entry name" value="DEP_RGS7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058855">
        <property type="entry name" value="RGS1/SST2-like_Fungal-DR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF192">
        <property type="entry name" value="DOUBLE HIT, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25889">
        <property type="entry name" value="WHD_Fungal_DR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0734">Signal transduction inhibitor</keyword>
    <feature type="domain" description="DEP" evidence="3">
        <location>
            <begin position="310"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="2">
        <location>
            <begin position="403"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="591" mass="67995" checksum="13CE25541939A165" modified="2011-11-16" version="1">MIISASIRSSPVFHDVVLNKFRIAPNGEIYERDIKDMFAMLIIHLDLKSIKSKRFVSLTKTFPYCFDCNTAVKKMKDMSISIEVSKIVTRISYKLHPEVSLSLLSKFYRSKFLHSPVERTASELTGNMVFQPTPKGLAIVQAFCERNGMKKCNLPEILNSNFNSMELFCFERDWESDKVLYSKYLLHLLFSSLMGPKPNVWSSSNEQDPLPSSQAEDHRLSTEFQTAKSFQEVEGFSFVQFRSAPSMSPGQLSPQPNSWSKTSKNEVSPFHHRYFTNPESDAHIQYYVSSVGVRLFKDKTFHLPKEKKLVVDYSMSGKAICQWLLDCTDVLNIRQAKDIADSILKNKLIKQIAPDQSSGGHFSSTRNSYYTITESGLSVSQWDKCPKAHFEFLTGKHSGKQLTLEDILKDPGMRLQFRMHLNREYCLENLEAYMHLKYFDKILNNYKKLLKYVHERSFRDEMVAQKIDRRLQQLKRTCSTLAYQIFITYLSDGAPSVVNIAYKLKASISDIFISTTQSNISELLLNEPNQTDTEEDIPTSDISSFVQLHDESIKLLSDVSVLFSQVSNHLYRLMEVDSMPRFLKGLSNRNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3APM2</accession>
    <name>G3APM2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphatidyl synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61276</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375469.1">
        <property type="nucleotide sequence ID" value="XM_007375407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APM2"/>
    <dbReference type="STRING" id="619300.G3APM2"/>
    <dbReference type="GeneID" id="18873813"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61276"/>
    <dbReference type="eggNOG" id="KOG1618">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030880_3_2_1"/>
    <dbReference type="InParanoid" id="G3APM2"/>
    <dbReference type="OMA" id="DIMKWNP"/>
    <dbReference type="OrthoDB" id="10251048at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000069">
        <property type="entry name" value="HAD-superfamily subfamily IIA hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000201">
        <property type="entry name" value="Piso0_001792 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050324">
        <property type="entry name" value="CDP-alcohol_PTase-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006353">
        <property type="entry name" value="HAD-SF_hydro_IIA_CECR5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01456">
        <property type="entry name" value="CECR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269:SF4">
        <property type="entry name" value="CAT EYE SYNDROME CRITICAL REGION PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="540"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="546"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="594" mass="66190" checksum="0B724E69ACA2EEA4" modified="2011-11-16" version="1">MSNGNFIVQEDGAASDSAIDSAPGSASGSALASPKFQSYLNNDGIDELQPSLRKHRVSNLSLSDLNQWQTGLHKLTSSPSLSNKGSSTNLKKVDSLAKLSRNSSIIRRKKKAVIDHERVASYAFCFDIDGVILRGPDTIPQAVDAIKLLNGENKYNIKVPYVFVTNGGGKPEKQRADDLSRRLQTTITEDQIIQGHTPMKDLVDVYDTVLVVGGVGNTCRRVAESYGFKNVYTPLDIMKWNPAVSPYHDLTTDEIACCKDVDFSKIKIQAILVFADSRNWAVDQQIILELLLSKDGVMGTESQTYHDGPEIYFAHSDFIWATNYKLSRYGMGALQVSIAALYREHTGQELKVNRFGKPQKGTFKFANKVLTHWRQGVLDEHLKKLSIHDPNASQADILINEEGEEIINQSKLESYHYSDSDDDTDDEINKVVGKREQKTDKITLELPPASTVYFVGDTPESDIRFANSHDSSWHSILVKTGVYQEGTVPKYKPKHMCDNVLEAVKYAIEREHAKELEEWNETAVDDEASSAKINFSDLVMTPSDTQESKKSEKSEKSVASVDELETVEVPGILSEQISKVKDIKVHSNLRNEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3APM3</accession>
    <name>G3APM3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">beta-glucosidase</fullName>
            <ecNumber evidence="3 6">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">BGL7</name>
        <name evidence="8" type="ORF">SPAPADRAFT_55707</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Glycan metabolism; cellulose degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="3 6"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375470.1">
        <property type="nucleotide sequence ID" value="XM_007375408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APM3"/>
    <dbReference type="STRING" id="619300.G3APM3"/>
    <dbReference type="GeneID" id="18871961"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55707"/>
    <dbReference type="eggNOG" id="ENOG502QR4D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004542_4_0_1"/>
    <dbReference type="InParanoid" id="G3APM3"/>
    <dbReference type="OMA" id="QIHYRED"/>
    <dbReference type="OrthoDB" id="47059at2759"/>
    <dbReference type="UniPathway" id="UPA00696"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.300:FF:000006">
        <property type="entry name" value="Beta-glucosidase H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000495">
        <property type="entry name" value="Periplasmic beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050288">
        <property type="entry name" value="Cellulose_deg_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019800">
        <property type="entry name" value="Glyco_hydro_3_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011658">
        <property type="entry name" value="PA14_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715">
        <property type="entry name" value="BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715:SF27">
        <property type="entry name" value="BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07691">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00758">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00775">
        <property type="entry name" value="GLYCOSYL_HYDROL_F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PA14" evidence="7">
        <location>
            <begin position="400"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="835" mass="92475" checksum="4B6E95B653B72A60" modified="2011-11-16" version="1">MTVSDFDVEATLARLTLEEKAKLLAGIDFWHTAPIHRVGIPSLRFSDGPNGLRGTKFFNSVPAACFPCGTGLAATFDKKLLEQAGVLMGEEAHHKGVHVILGPTMNIQRGPLGGRGFESFSEDPYLTGVAAGAIIKGIESKGIAATPKHFVCNDLEHERNASDSIVTQRALREIYLEPFRIALRDGKPSALMTSYNKVNGTHCSQNEHLLQEILREEWEWQGTVMSDWWGTYTTKESLANGLDIEMPGPTYFRKVEAICHMVKTRELHINKLDDRARSVLRLIKVALQSGIPEGGKETTQNNTPETSALLRKLASDSVVLLKNEGNILPLKPEETVAVIGPNAKVAAYCGGGSAALRAYYTTTPYDSIAAKANTKPKYTVGAYAHRLLPALGPRLINPKTGQTGYNMKFYLEPRGTPQRTLVDEYNLDISLLHFPDYYNKKVKDNLFYIDFEGDFTPEESGDYEFGVTVVGTAQLFIDDKLVVDNKTKQRHGETFFNVGTVEEHGIIELEKGKTYKVRVEFGSGPTYTLPSNGCEFFGGSINLGMAKVIDDDDEIANAVKIAQSVDKVVVCIGLNQEWESESFDRPHMKLEGYQDKLVEAILAANPNTVVVNQSGTPVELPWVDQAPCLLQAWYGGNESGNGIADVLYGDSNPSGKLTLSFPKRNEDNPAFLNFRTERGRVLYGEDIFVGYRYYEKLGREVAFPFGYGLSYTTFDFSNLKVDVNQNTLQVAVQVANTGKLDGSEVVQVYISKDDSDVIRPVKELKGFEKVHLKAGAKDTVVIELPLKDSVSFWDEYQDKWSVEAGEYHVLVGNSSDHTPLRASFTIDQSFFWKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3APR9</accession>
    <name>G3APR9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Casein kinase II subunit beta</fullName>
            <shortName evidence="3">CK II beta</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_61320</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3">Regulatory subunit of casein kinase II/CK2. As part of the kinase complex regulates the basal catalytic activity of the alpha subunit a constitutively active serine/threonine-protein kinase that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the casein kinase 2 subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375516.1">
        <property type="nucleotide sequence ID" value="XM_007375454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APR9"/>
    <dbReference type="FunCoup" id="G3APR9">
        <property type="interactions" value="1035"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APR9"/>
    <dbReference type="GeneID" id="18873840"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61320"/>
    <dbReference type="eggNOG" id="KOG3092">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034027_3_2_1"/>
    <dbReference type="InParanoid" id="G3APR9"/>
    <dbReference type="OMA" id="DADFGRC"/>
    <dbReference type="OrthoDB" id="3971593at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030291">
        <property type="term" value="F:protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006356">
        <property type="term" value="P:regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006359">
        <property type="term" value="P:regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1820.10:FF:000010">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000001">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1820.10">
        <property type="entry name" value="protein kinase ck2 holoenzyme, chain C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016149">
        <property type="entry name" value="Casein_kin_II_reg-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035991">
        <property type="entry name" value="Casein_kinase_II_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000704">
        <property type="entry name" value="Casein_kinase_II_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740:SF39">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01214">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00472">
        <property type="entry name" value="CASNKINASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM01085">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57798">
        <property type="entry name" value="Casein kinase II beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01101">
        <property type="entry name" value="CK2_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00045899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="242" mass="28173" checksum="0A7A94531D9930D2" modified="2011-11-16" version="1">MADYITDSSSDFTEYWIDLFLGIKGNEYFCDIDDEYIRDRFNLTGLNQEVSKLPTLIDIITDLIDLDSQPEDQRDTLEHNARILYGLIHARYILTSRGLNKMFEKFRNGDFGYCPRINCQLHPLLPIGLNDSPRVASVKLYCAKCEDLFNPKSNRHAVIDGAYFGTSFPAMFFQNFPNAIPIHSKETYVPRVFGFKLHEYSQLNRWRHLQRMKLESRLVKSGINVSNVNGGFIEVEQRQEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3APW4</accession>
    <name>G3APW4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_71739</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375561.1">
        <property type="nucleotide sequence ID" value="XM_007375499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APW4"/>
    <dbReference type="STRING" id="619300.G3APW4"/>
    <dbReference type="GeneID" id="18875395"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_71739"/>
    <dbReference type="eggNOG" id="KOG0667">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_5_10_1"/>
    <dbReference type="InParanoid" id="G3APW4"/>
    <dbReference type="OMA" id="FRGHMCI"/>
    <dbReference type="OrthoDB" id="9332038at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF22">
        <property type="entry name" value="DUAL SPECIFICITY TYROSINE-PHOSPHORYLATION-REGULATED KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="333"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="106"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="142"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="678" mass="77464" checksum="FE9A328C09982BEA" modified="2011-11-16" version="1">MTEFEDRFSQINIGKSPTRRKKAYPQHLYPTPTRDSEPHRRTPQSTLPPTPQLSNPPHTPSRAISNELKSKSTINHQDHRIISYPMSEQQGSKKQFQFSFMRETWSSQQKKNIGPLTNTTNNPLTMSKPVVPTRKRSEQRKSSQTSRPRRLASNTVPIKLQLSQATNILSDFEISSPTKPKTKYHHPNAASPIKLPTATHFNPPTRTASNSSDNVFNRLYPKHEQEAPPRTIKRTRPKISNIPELYNILSRKQPDLFKCEDPPVLNSPIPVDQVPMNSLNIYERGEIIRKSELYYVPTECRTIDVKNSANNFGFDDNKGHYIIIPQDHINYRFQILDTLGNGSFGNVILAKDHKLNSTVAVKVINNNLNWSLQAVSEIKLLKLLNEKESNENILRYITHFNFRSHMCIATELLSLNLYTLLELTNFQGLSIDIIKIFTKQILNGIQYLHKYNIIHCDIKPENIMIKLPSTPNSTDISIKLIDFGSACYTNETSFTYIQSRFYRAPEVILGSNYNEKIDIWSFGCVITELFMGSPLFPGKSEIEQVGLILELLGSPKSGTILRMRRHLTRSHGDNHYLENSLSIPNEKQLKRTLLFKIFDINGKLNLQLVNSISAATGIKRQFKVNSRGLDIVMGLNKPGMTMSPYNKSTLLRFLGKIFIWDPIERAGASELLQEPFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3APZ2</accession>
    <name>G3APZ2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_66942</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375589.1">
        <property type="nucleotide sequence ID" value="XM_007375527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APZ2"/>
    <dbReference type="STRING" id="619300.G3APZ2"/>
    <dbReference type="GeneID" id="18875126"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66942"/>
    <dbReference type="HOGENOM" id="CLU_894770_0_0_1"/>
    <dbReference type="InParanoid" id="G3APZ2"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="311" mass="36116" checksum="BB6CDAAAF9C0237E" modified="2011-11-16" version="1">MALGSVIAQLQSAGKRKRLRKSAFQIVSHCRKFPKVSLSRKVNQCRNYHTISLIGKLTPEGSNLLDVLFMTSFPNRDLLNPEGSDGGIWVSITEPFARSPERRFAGVVSTWNNCRFKDKFNENAELFKACVADVYIFSSNSENEFCWNELKVFFKNTLVLFGQSRIDKYNCHDHRALLLVVHRSRYRNIYDIDAFESKVRDIWKEIYNTSDFDNYFVIQSHTISPTNYMKDIDTIKSCLMVNQFLRANQCEFKIPFAEWLQNASKCWEYLSTTPPPYESQEQTRKDVPLSRTEKSIAEFSFAILGFRSDGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ04</accession>
    <name>G3AQ04_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_66952</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375601.1">
        <property type="nucleotide sequence ID" value="XM_007375539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ04"/>
    <dbReference type="GeneID" id="18875127"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66952"/>
    <dbReference type="HOGENOM" id="CLU_057903_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQ04"/>
    <dbReference type="OrthoDB" id="4016968at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442652" description="F-box domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="492" mass="57610" checksum="56056C363A5046D5" modified="2011-11-16" version="1">MLLPILCNCVIVLVLIIIKQQAGVLCRSKQEQKSELKSELKSELKSELKSELIQLVNEVRQIRQEIEEIRVKKPKSAIEKIKITIMSLSDDIFLNVLRYLNQIHVCRICTINSRLYNLGKIKLYRCIYFHIRSLNTIHISANTYTSFYTNFTIVNKWNTFPWISENSNITLVKKMVFRVNGLLFDIVKTQYPWIEVIAEDQNGYYHSQYPLQEYHYTQLILSRRVGIPFLNGCQEMPWVKKLTIYLIVVPEQSLNEIKYLSGLEDLGIDVVDTDDLEELCDIGLGDMPIKKLRISVSELIDMEELQEITEIFDLEKITMFQLHLWDEDTYPENFNSSIQKLLSALVNVTSLSIICQNIDFEKVLESMKPNSLHTLHLKIVQVGNDIHTFQLENILNYQEHSLRRLYLSNNKQRDKTKMYGLQEFERIYKKETKPLIPNQQPVGIFEIRRMIDAGKFQKLNRVLINESCYFIKRPSNGMIHLVPCNSLIPDNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ76</accession>
    <name>G3AQ76_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Prohibitin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_61989</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Prohibitin probably acts as a holdase/unfoldase for the stabilization of newly synthesized mitochondrial proteins. Involved in mitophagy; may act as an adapter for ATG8 that supports mitophagosome assembly. Negatively regulates the proteolytic processing of ATG32 via the i-AAA protease. Acts as a negative regulator of the m-AAA protease.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">The mitochondrial prohibitin complex consists of two subunits (PHB1 and PHB2). The subunits assemble into a membrane-associated ring-shaped supercomplex of approximately 1 mDa. The mitochondrial prohibitin complex interacts with the m-AAA protease, a heterohexamer composed of YTA12/RCA1 and YTA10/AFG3. The mitochondrial prohibitin complex interacts with ATG8 and the interaction may support mitophagosome assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the prohibitin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376201.1">
        <property type="nucleotide sequence ID" value="XM_007376139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ76"/>
    <dbReference type="FunCoup" id="G3AQ76">
        <property type="interactions" value="921"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ76"/>
    <dbReference type="GeneID" id="18874137"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61989"/>
    <dbReference type="eggNOG" id="KOG3090">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047969_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQ76"/>
    <dbReference type="OMA" id="VPFIQNP"/>
    <dbReference type="OrthoDB" id="275637at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035632">
        <property type="term" value="C:mitochondrial prohibitin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140580">
        <property type="term" value="F:mitochondrion autophagosome adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045861">
        <property type="term" value="P:negative regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03401">
        <property type="entry name" value="SPFH_prohibitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000001">
        <property type="entry name" value="Prohibitin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000163">
        <property type="entry name" value="Prohibitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222">
        <property type="entry name" value="PROHIBITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222:SF1">
        <property type="entry name" value="PROHIBITIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00679">
        <property type="entry name" value="PROHIBITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="Band 7" evidence="9">
        <location>
            <begin position="56"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00063239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="253" mass="28210" checksum="7786509EF5A98D5C" modified="2011-11-16" version="1" fragment="single">MNDPNWERLAKQFKRVSQQTRSGGSGGKMPRSPLAMFSGIGGLVLLAGGTFLVQNALFNVDGGQRAIVYSRVNGVQSQIYPEGTHFVIPWFQRPIIFDVRAKPREIASLTGTKDLQMVNITCRVLFKPDMFQLPTIFRTLGSDYEEKVLPSIVNEVLKSVVAQFNASQLITQREKVSRLVKENLIRRASKFNILLDDVSLTFMTFSPEFSAAVEAKQIAQQDAQRAAFIVDKAIQEKQQLVVKAMGEAKSAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQD8</accession>
    <name>G3AQD8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_51493</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376264.1">
        <property type="nucleotide sequence ID" value="XM_007376202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQD8"/>
    <dbReference type="GeneID" id="18871561"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51493"/>
    <dbReference type="eggNOG" id="ENOG502RQCA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1255817_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQD8"/>
    <dbReference type="OMA" id="CHAENIM"/>
    <dbReference type="OrthoDB" id="4083723at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="189" mass="21086" checksum="58B0B94731B47E41" modified="2011-11-16" version="1">MNTLDYRFPKTYTPENHDKLPLDVQLNRNVRIDLPKPKPIQYITLPIAQSDDPLDGILSQCKGLIQGYSLELPKEKSTKRKREYEEPITPKHARITPSTTPKSSTPVAILKQAQKGISADYDDSDYDHLACHADNILRMAVDSTMLGSTLGSITFANDSIYEKGLVFSKAVKIDINADELNAAVNDELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQF4</accession>
    <name>G3AQF4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_139977</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376279.1">
        <property type="nucleotide sequence ID" value="XM_007376217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQF4"/>
    <dbReference type="STRING" id="619300.G3AQF4"/>
    <dbReference type="GeneID" id="18870258"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139977"/>
    <dbReference type="eggNOG" id="ENOG502S5GS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1102649_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQF4"/>
    <dbReference type="OMA" id="HDECAII"/>
    <dbReference type="OrthoDB" id="4021635at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="221" mass="25449" checksum="78AD9AF00EFB6477" modified="2011-11-16" version="1">MIKEPYYTSISSIKPKNANNYSLIAYDLPIIQPTLQNAPRQEVNDVVRQHRHHLHHRKDSIEPYYSKPRRLSGLPPVILNQFMYKDESEDDSCAVISDDEDECIDNHVVNPPKSLLRTVSDNYDSETEEEVNFAKLMQPIGALPKVDKTCSDCEDLSEVNEILLAQVNNPNILINFRERTLSNGLSPPLIHQTLFSNDNYDVVYKSEIRDDYDGNHCEYLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQL5</accession>
    <name>G3AQL5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription factor MBP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_140662</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to MCB elements (Mlu I cell cycle box) found in the promoter of most DNA synthesis genes. Transcriptional activation by MBF has an important role in the transition from G1 to S phase. It may have a dual role in that it behaves as an activator of transcription at the G1-S boundary and as a repressor during other stages of the cell cycle.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376340.1">
        <property type="nucleotide sequence ID" value="XM_007376278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQL5"/>
    <dbReference type="FunCoup" id="G3AQL5">
        <property type="interactions" value="784"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQL5"/>
    <dbReference type="GeneID" id="18870311"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140662"/>
    <dbReference type="eggNOG" id="KOG4177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009666_2_0_1"/>
    <dbReference type="InParanoid" id="G3AQL5"/>
    <dbReference type="OMA" id="ASHNYVL"/>
    <dbReference type="OrthoDB" id="6718656at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030907">
        <property type="term" value="C:MBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033309">
        <property type="term" value="C:SBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.260.10:FF:000004">
        <property type="entry name" value="Transcription factor MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.10">
        <property type="entry name" value="Transcription regulator HTH, APSES-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018004">
        <property type="entry name" value="KilA/APSES_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051642">
        <property type="entry name" value="SWI6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828">
        <property type="entry name" value="ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828:SF15">
        <property type="entry name" value="TRANSCRIPTION FACTOR MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13606">
        <property type="entry name" value="Ank_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04383">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01252">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HTH APSES-type" evidence="7">
        <location>
            <begin position="6"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="111"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="242"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00073969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="853" mass="97208" checksum="8264C4D8095DA782" modified="2011-11-16" version="1">MANTQIYSATYSNVPVFEFVTSEGPIMRRKSDSWINATHILKIAKFPKAKRTRILEKDVQIGVHEKVQGGYGKYQGTYVPLDFGEQIAKNFGVYEVLRPIFEFRYIEGKSETPPPAPKHNHASALNVAKRQAQQQQQQQQQQQQQQQQSHAGNKRTKSGSPLEPKKRGRPKRVALSQTPTLKHSDTTPLEPQVSGNYTGYNPNGGASTIKTSFVGMLPALTRQDTEQDAPLHLMNMNLKQEDLQAVDSEVDDDDRNEERYYPSGNGDLHPLVSHHDDLLTSKELFGVSRESFEKPGRPAGPNDPYSLQQYHQPSGFSSSQDIYSKYFSNLLTYFLDEDNNKARTAIPESLVNPPQPLSKINIDQPIDNDGNTLFHWACSMANIPMIEFLLSTFSEFITPDIRNNHGETPLMFLVRFNNSYQLHNFPSILQLLLESILLVDASGKTVLHHIVHIDHEGNKSDKKKANKEKFARYYMECLFDKIIEEEGHTENDEEEGEPEGDQEKKQLIAKFINHQDADGNTAFHIAGYNLNKKCIKVFISYHRYINFNLRNLVSCTVEDYLASHNYVLRLDEEDKDDDQDEENGGVSTSEHLLVSSANGSRFNNQSFEANLYKSKLAVNLHNTTSNLITEKMAELAYIVDKELSEKDEVILTYFKILQKVNQDKLASQREVLSLFKLDFLIDELEKEGRGNVDESSQESSSQQSDLNIDLSKNQIVQEEIYRLINDLTFQYLQKRNDLDEVMSRYKEVKAKAHAAALNRLISSYQDDGEDVDKFALTQELQSQIIKQRQLSDQIFNQEKDVPRCREGHNSIMSKYGDNDKLSKYCQLIAICCGMGIDEVESSIDLIEQSLTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQS1</accession>
    <name>G3AQS1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase C-like 1</fullName>
            <ecNumber evidence="2">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">SPAPADRAFT_141197</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="2"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376396.1">
        <property type="nucleotide sequence ID" value="XM_007376334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQS1"/>
    <dbReference type="FunCoup" id="G3AQS1">
        <property type="interactions" value="429"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQS1"/>
    <dbReference type="GeneID" id="18870353"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141197"/>
    <dbReference type="eggNOG" id="KOG0694">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_54_1_1"/>
    <dbReference type="InParanoid" id="G3AQS1"/>
    <dbReference type="OMA" id="QCTHLVP"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000935">
        <property type="term" value="C:division septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032186">
        <property type="term" value="P:cellular bud neck septin ring organization"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902660">
        <property type="term" value="P:negative regulation of glucose mediated signaling pathway"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000425">
        <property type="term" value="P:pexophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903139">
        <property type="term" value="P:positive regulation of cell integrity MAPK cascade"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010606">
        <property type="term" value="P:positive regulation of cytoplasmic mRNA processing body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090334">
        <property type="term" value="P:regulation of cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000769">
        <property type="term" value="P:regulation of establishment or maintenance of cell polarity regulating cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070610">
        <property type="term" value="P:regulation of fungal-type cell wall (1-&gt;3)-alpha-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060211">
        <property type="term" value="P:regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034063">
        <property type="term" value="P:stress granule assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd20822">
        <property type="entry name" value="C1_ScPKC1-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20823">
        <property type="entry name" value="C1_ScPKC1-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08689">
        <property type="entry name" value="C2_fungal_Pkc1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11620">
        <property type="entry name" value="HR1_PKC-like_2_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05570">
        <property type="entry name" value="STKc_PKC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000101">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000014">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000034">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000474">
        <property type="entry name" value="Serine/threonine-protein kinase N2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037778">
        <property type="entry name" value="C2_fungal_PKC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036274">
        <property type="entry name" value="HR1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037312">
        <property type="entry name" value="PKC-like_HR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02185">
        <property type="entry name" value="HR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00742">
        <property type="entry name" value="Hr1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46585">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="REM-1" evidence="22">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="22">
        <location>
            <begin position="99"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="20">
        <location>
            <begin position="412"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="20">
        <location>
            <begin position="477"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="729"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="21">
        <location>
            <begin position="989"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="321"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="548"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="640"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="331"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="649"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="758"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="EGW31618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1056" mass="120936" checksum="0F35377555BC35D2" modified="2011-11-16" version="1">MSQQQKTEDVIKDIKSKIEVEKNIVEGYQNVKRMSQNAEVVQKCNSKIIESQSIIDYYQDSLNKLTRQLQDLNTNGHPTNGNRNSGTERPSYSRLDLIKYECPSVGHKIHHMLQQLQFKLQVENQYREANKKISHLYLMDGDRSSSDAAEGGKAESDQRIQLLEKALKKYQNIHIDSEDFTRDFALINTPKHARKPLSGRLFISVTCIRDVDHISSPVFAKKRESVIVIKIDDLERARTKPSKTDKWTEEFVVNVEKGHEIELCVLDKSGNNMVPVAMNWLLLSDVAEEIRKRKVAKELGNSGWITASNLEGANVPSRGASFGATLPLPPTSSAGSTSPTTSAFATPAPTPAAAGSPDKIQLNTWLSLEPCGQMLVSMNFEKSTVPGQKFMGPLERHGAIRQKKEEVFEQQGHHFVQKQFYNIMCCALCGEFLRYTGYQCQDCKFLCHKKCYSKVVTKCISKSGIDYDETKLKHQIPHRFEPITNHGTKWCCHCGYILPWGKKNVRKCTECGVMCHAQCTHLVPDFCGMSMEMANEILATIKSTKVSPKKPQKLNLAPKSEYSLKPQVSPLPVKEERRMSEDDEAKTRLATQTTNMVQEPIRLPHHIAKPKQYPSEKRPSFEADMSFDQNRENEIQFRRHDRVQQQQEQRLEAVTEDQRKSLESSKNHFDQFDYQNVQTQDQIMADVDQTKNPFQDQYHHADVTAGHITKPHKSHKQKRKRRKVGLDDFQFLAVLGKGNFGKVMLAESRHTSTLCAIKVLKKDFIVENDEAESVKSEKRVFLTANKEKHPFLLNLHCCFQTENRIYFVMEYISGGDLMWHIQKSRFPAKRAKFYACEVLLGLKYFHDNGIVYRDLKLDNILLTTKGHIKIGDYGLCKENMWYQSTTSTFCGTPEFMAPEIVAGKQYDRSVDWWAFGVLLFQMLLCQSPFKGDDEDDIFNAIEHDEVKYPISLSRQTVLVLQALLTKDPSQRLGSGPRDAEEIMEHPYFHDVNFDDVFNLRIPAPYIPEVQSEHDYSNFDQEFTSETPRLTPVETVLTAEMQEQFRGFSHIAENAVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQW5</accession>
    <name>G3AQW5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_139862</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375972.1">
        <property type="nucleotide sequence ID" value="XM_007375910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQW5"/>
    <dbReference type="STRING" id="619300.G3AQW5"/>
    <dbReference type="GeneID" id="18870245"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139862"/>
    <dbReference type="eggNOG" id="KOG1944">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066033_2_0_1"/>
    <dbReference type="InParanoid" id="G3AQW5"/>
    <dbReference type="OMA" id="KMAIYGA"/>
    <dbReference type="OrthoDB" id="860at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF93">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN 25D9-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="218" mass="24301" checksum="2B282B99141A561A" modified="2011-11-16" version="1">MPSLKQANAKYVESIIKYPLLTKAITGAILNGLNEIISTTVTSDYSTTEILGYKVNHVLSPKLIKMIIYGGLISTPVSHYMYHIINNKIFTGQLSKLGKILQLLTSLLTVTPTICGIFVSWVSLINNYKVDKGEFNLKKEVAKMVGIVKTGLQRGYKGILKTALVTSFVSLIVAQKYIPQELWVVFFSSVSFVLGTIQNIKLKKLQKQARLDKEEKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR31</accession>
    <name>G3AR31_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_62298</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376470.1">
        <property type="nucleotide sequence ID" value="XM_007376408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR31"/>
    <dbReference type="FunCoup" id="G3AR31">
        <property type="interactions" value="701"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR31"/>
    <dbReference type="GeneID" id="18874257"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62298"/>
    <dbReference type="eggNOG" id="KOG1165">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019279_1_0_1"/>
    <dbReference type="InParanoid" id="G3AR31"/>
    <dbReference type="OMA" id="DWMHLNG"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000968">
        <property type="entry name" value="Casein kinase I, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000538">
        <property type="entry name" value="Putative Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="81"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="25"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="378"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="449"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="59"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="397"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="456"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="514" mass="58393" checksum="6A07399B7F461D37" modified="2011-11-16" version="1">MSNRQQFTSNPAAIAAAQASVPLTSPLKMNNSNSHLNNSSTGGHHHHHHHNGGIASGASNVANSNTQSNSNNNSSVVGLHYKIGKKIGEGSFGVIFEGTNIINGVPVAIKFEPRKTEAPQLRDEYRTYKHLQGCEGIPNAYYFGQEGLHNILVIDLLGPSLEDLFDWCGRRFSVKTVVQIAIQMLTLVEEVHRHDLIYRDIKPDNFLIGRQGLPDENHVHLIDFGMAKQYRDPRTKQHIPYREKKSLSGTARYMSINTHLGREQSRRDDLEALGHVFFYFLRGQLPWQGLKAPTNKQKYEKIGDKKRTTPAVTLCDGLPQQFAEYLDSVRSLPFDAEPAYEEYRMLLLSVLDDLGQSCDGDYDWLHLNGGKGWDASINKKPNLHGYGHPNPPNERERRHRDQRRSRQAQQQLQQQQQQQGQPIQAQQQQQQQQQTVQLHQQKLQHLVNRPLPPIKQESQQGVTSSGGPDLMNNMTNQAGGKYEGYNNRQDHYQQQQIIDDNQESKGFWSKLCCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR85</accession>
    <name>G3AR85_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Coatomer subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_139873</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus membrane</location>
            <topology evidence="1 10">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376038.1">
        <property type="nucleotide sequence ID" value="XM_007375976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR85"/>
    <dbReference type="FunCoup" id="G3AR85">
        <property type="interactions" value="1300"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR85"/>
    <dbReference type="GeneID" id="18870247"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139873"/>
    <dbReference type="eggNOG" id="KOG0292">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007565_1_0_1"/>
    <dbReference type="InParanoid" id="G3AR85"/>
    <dbReference type="OMA" id="EMTYQKQ"/>
    <dbReference type="OrthoDB" id="10261470at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22948">
        <property type="entry name" value="Coatomer_WDAD_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000002">
        <property type="entry name" value="Coatomer subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000022">
        <property type="entry name" value="Coatomer subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006692">
        <property type="entry name" value="Beta-prop_COPA/B_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047312">
        <property type="entry name" value="Coatomer_alpha_WD-assoc_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016391">
        <property type="entry name" value="Coatomer_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010714">
        <property type="entry name" value="Coatomer_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF1">
        <property type="entry name" value="COATOMER SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04053">
        <property type="entry name" value="B-prop_COPA_B_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06957">
        <property type="entry name" value="COPI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003354">
        <property type="entry name" value="Coatomer_alpha_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <keyword evidence="4 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="49"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="91"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="133"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="209"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="253"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B second beta-propeller" evidence="13">
        <location>
            <begin position="347"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="15">
        <location>
            <begin position="634"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer alpha subunit C-terminal" evidence="14">
        <location>
            <begin position="827"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="841"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="848"/>
            <end position="861"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR003354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF06957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW31260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1222" mass="137946" checksum="0F8BE9B3CBE0ADBC" modified="2011-11-16" version="1">MKMLTKFESKSSRAKGIAFHPKRPWVLVSLHSSTIQLWDYRMGTLIDRFEDHVGPVRSVDFHPTQPLFVSGGDDYSIKVWSLVTRKCIFTLNGHLDYIRQVSFHPDLPWILSCSDDQTIRIWNWQNRQEIACLTGHNHYVMSAQFHPTEDLIVSASLDQTVRVWDISGLRKKHSAPASSVRSFEDQLQRQQLPQQDIFGNINAVVKYVLEGHDKGVNYASFHPTLPLIVSSGDDRVVKLWRMSDTKAWEVDTFRGHTGNVLCAIFHPHQDLILSVSDDKTVRVWDLNKRVPVKQFRRDHDRFWLIGAHPNMNLFGACHDSGVMVFKLERERPAHTIFQNKLYYINSEKQLQSYDYSRNETSLPMLSLKKIGKTWSFMRTISYNQTDNSILVVFGEGDSSNYALISLPKHVTGAIEPTDIRQGEANFATFISRNRFVTFIKSTKTLHVKDMSNNTTKTIQLDSSVNDVLPGQGGRILLVKAHSVINYDVQQRKEVAELSVNNVKYVSWSNDGTYVAMLSKHTITIANRDLELVTSMHETIRIKSAAWDDTGVLLYSTLNHIKYTLLNGDNGIIKTLENTLYITKVVGNQVYCLNRDGAVEVIAIDPTEYRFKKSLVNKNFHEVLRLIKNSTLVGQNIIAYLQKKGFPEVALHFVQDPETRFDLALECSNLQVAFEQAKILNDSQIWEKLGEEALLQGNLEIVEYVYQQLHNFDKLSFLYLYKGDNERLNKMTTIAEHREDTSALIQNTLYSNDVKKRCQVYIQNGMLPLAYTLAKSNGLVELAAQILQEAGLEEKDVDLPELGGVVQLPTPIEEPIGNWPLKESSLSFFETALITGQVENLSLEDEPEQQQQPQQQQQQQQQFTKSATPALEFNDEEFEDDEDDGGAWGDLDDDLDIGDEFNAQAAAGAPSPDVVPVVEGEIGMWLRNAKTPASYAAAGAFEQAASLLNKQLGVVNFAPLRQRFLTVYGANKVYLPGADDLPGIKTFIRAENDEDDPKKFKPFIPGFEQLDELVKIGFRHFKLNHLEDAISTFRDIIYTIIVLNVEDEDEEEKCRQILSLCREYILGLSIELARRALDPSDIKRNLELAAYFTRTQLQTDHRVNALKVALTQSMKAKNYVSAAHFAGEIIKISPAGSKNYVQAESFKLRAETAGSEAVEIDFDPYSDFEICSGTFTPIYPGEPSVSEAIVGAKYKPEFKGGVCSITGVSAIGVPASGLRIRAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARG1</accession>
    <name>G3ARG1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribose-5-phosphate isomerase</fullName>
            <ecNumber evidence="5">5.3.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">D-ribose-5-phosphate ketol-isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Phosphoriboisomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RKI1</name>
        <name evidence="11" type="ORF">SPAPADRAFT_139889</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14657"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="EC" id="5.3.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ribose 5-phosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="5"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376060.1">
        <property type="nucleotide sequence ID" value="XM_007375998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARG1"/>
    <dbReference type="FunCoup" id="G3ARG1">
        <property type="interactions" value="931"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARG1"/>
    <dbReference type="GeneID" id="18870249"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139889"/>
    <dbReference type="eggNOG" id="KOG3075">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056590_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARG1"/>
    <dbReference type="OMA" id="ACHVQEK"/>
    <dbReference type="OrthoDB" id="1555531at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00412"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006014">
        <property type="term" value="P:D-ribose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000014">
        <property type="entry name" value="Ribose 5-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000053">
        <property type="entry name" value="Ribose-5-phosphate isomerase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001924">
        <property type="entry name" value="PRK00702.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934:SF0">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75445">
        <property type="entry name" value="D-ribose-5-phosphate isomerase (RpiA), lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW31282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="234" mass="25183" checksum="5B64B39D18C68A55" modified="2011-11-16" version="1">MSSVESAKKLAAYRAVDENFPPAAKVVGIGSGSTVIYVAERIGQLDNKSDFVCIPTGFQSQQLIIDNGLRLGTIEQFPHIDIAFDGADEVAPNLDCIKGGGACLFQEKLVAAAADKFIVVADYRKKSEVLGRQWTQGIPIEIVPNAYAKVINDLKKLGAKKVDLRSGGKAKAGPVITDNHNFLLDADFGDVKDAKKLHDEIKALVGVVETGLFTDLAQKAYFGEENGEVSTWTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARK8</accession>
    <name>G3ARK8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ATP-dependent permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_155363</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376539.1">
        <property type="nucleotide sequence ID" value="XM_007376477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARK8"/>
    <dbReference type="FunCoup" id="G3ARK8">
        <property type="interactions" value="791"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARK8"/>
    <dbReference type="GeneID" id="18871018"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_155363"/>
    <dbReference type="eggNOG" id="KOG0055">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_17_8_1"/>
    <dbReference type="InParanoid" id="G3ARK8"/>
    <dbReference type="OMA" id="TFWACLT"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015440">
        <property type="term" value="F:ABC-type peptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000770">
        <property type="term" value="P:peptide pheromone export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03228">
        <property type="entry name" value="ABCC_MRP_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000604">
        <property type="entry name" value="ABC transporter B family member 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF15">
        <property type="entry name" value="ALPHA-FACTOR-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="65"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="283"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="654"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="703"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="777"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="803"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="887"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="21"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="351"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="657"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="981"/>
            <end position="1221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW31761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1223" mass="137166" checksum="E23976981D08BC8F" modified="2011-11-16" version="1">MPSPNRNILMFFKKEDIPLAILGTILMCFSAVSTPLSTMIYGDTIGCLGEFYTGQLNYHEFITKVRILCGALMGIGAAKMILVWFGISVWMRFGEVQQLRARKQLFNKLLHEEIKWYDRKQNLMGNISQVNRCIEELRGGNAEVMAMLVQTFALIIALVIMSFYQSWAITLVILASAPVMAFSGWWFGRLTYKAAEVENETTAEGSKVLDWCLVSPEIVRVFNGKYVEFSKFKAIVNSSAKAYYKLANAISANTAVLKFLTLMMFVQAFWFGIYLMSKGTITINQLITCFGSCLMLGSTVSGVTELLAVLNTAHAAAGKISTFLDMKDKDKDKFDFRENLNIYPSTCNGNISFNHVYFQYPSRDELILKDITFTLEPNTMNFIIGKSGSGKSTIPLLLMNIYDVQCGSIEIDSHPIQLLDPKYITQNLTLVQQNPTIFNKSIRENIALGVSDFFHQLEHVPEHLISDAAEFSLLHDLIEDKGLDVQVSSSSLSGGQQQRIAIARAKLRDPPILILDEAFSALDFKNRNLLFDKIRQWRTGKTTIVITHEFNNIESEDYVILTEGGKVVSEGPFRVLQDEHFIQAYNFDETEVESQETLTPDYKELPKSIYYNYKTSPYILKDLENQKVKEEEKENIMGVFAILKYCSKSIETKWVIVVGIIISILNGATNPIFSSCFAQLLSSSIDASMGIGVNEELLKWSCISIGVAFFIGLSGYVSQFILAYASERWIVSLRKTLFERINGQDMSFFDSEVVKPAEITALLMNDTRDLRNLISEFLQLIVNLITMVLVGLIWAIVVGWKLSLVGIAFVPLVLILTGFYGLVLNSTENDYKSSIAKLENNLYQVVSTIKTIRIYNMLRYFSNSFDNRVHEVQNVGIGRAIKTGIGLAVNDLLASVATATVLYFGMTLAGRSEYTSNQFMQVVSLLTFTLTNASSLLSQLPEISRGQRAGTLIVKLLENSALSKVENNGILKPTVVPDNIITFDHIEFAYPFKKQRILCDFSCEIQKNELIGIVGESGAGKSTIVSLLTRLYGTHSNQVKFFDEDIASVDIDWLRYTIGLVPQFPKFFEGTIYENLTYGMSPSVQISYPLLEEVLKMVGIYDFVVTLPEGLQTQIGEGSNSLVSGGQLQRLSIARALIRRPKVLIFDECTSNLDPLNSQHIIELIKKQLAGNYTIIIITHDKEMMKITDRLIVLKGGRVCESGKYNQLLATNGELTRITRSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARL6</accession>
    <name>G3ARL6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_51748</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376547.1">
        <property type="nucleotide sequence ID" value="XM_007376485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARL6"/>
    <dbReference type="FunCoup" id="G3ARL6">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="GeneID" id="18871591"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51748"/>
    <dbReference type="eggNOG" id="ENOG502QPK1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_1_1"/>
    <dbReference type="InParanoid" id="G3ARL6"/>
    <dbReference type="OMA" id="YMNERSP"/>
    <dbReference type="OrthoDB" id="5296287at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015355">
        <property type="term" value="F:secondary active monocarboxylate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035879">
        <property type="term" value="P:plasma membrane lactate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="354"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="382"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="406"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="477"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="96"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="518"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="529"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="544"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="563" mass="63110" checksum="49903834D35B3636" modified="2011-11-16" version="1">MSERNETNHLSRVSTRMSTISQRLPVSDIYTEPPKDLDHQLFNDKPDFSKKSINRYLSTRFSTLMDFPLYHSGKKWYQVVNPIPGLKEMTGSDWNFYFMGFFAWTLDAMDFFCVSVAAPEIASSLGVSVTDITWGVTLVLMLRSVGAIIFGIASDYFGRKWTYILILILFIVVEIGTGFVQTFSQFLGVRAIFGILMGSMYPVAMVTALEGQPTRARSILSGLFLPGYSLGYTLASVFYRAFAGTYKPGEGWRSLFWFSGALSVLLIAWRVCFPESPDYVAMKSKKREYNSKVEKKGFFSKFDYSVITTLKTEWLMFTYLVLLYAGFNFTTHGTQDLYVTLLTKQFNVGLNKKTVIIVVSNIAGILGGTTMGTVSELLGRRMTIVVCSIWCCVWLYPSFFNAHINWWAYVLLNFGVMGGWGVGPIHLLELVNSTHRTLLSGLAYQLGNLVSSASATIEAKIGERFPIKGGAPDVYDYGKVMCIFSAAVFIYMIICVFLGPERFHKELRLRDEIVGHGDEESELGRKASPSESLSSNNKELSSPNHNESPSDNNRDSSSITKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARU0</accession>
    <name>G3ARU0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_154380</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376135.1">
        <property type="nucleotide sequence ID" value="XM_007376073.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3ARU0">
        <property type="interactions" value="1052"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARU0"/>
    <dbReference type="GeneID" id="18870979"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_154380"/>
    <dbReference type="eggNOG" id="KOG1823">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000327_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARU0"/>
    <dbReference type="OMA" id="EGLMAMF"/>
    <dbReference type="OrthoDB" id="360653at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052575">
        <property type="entry name" value="SSU_processome_comp_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057525">
        <property type="entry name" value="UTP20_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046523">
        <property type="entry name" value="UTP20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011430">
        <property type="entry name" value="UTP20_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17695">
        <property type="entry name" value="SMALL SUBUNIT PROCESSOME COMPONENT 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17695:SF11">
        <property type="entry name" value="SMALL SUBUNIT PROCESSOME COMPONENT 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20416">
        <property type="entry name" value="UTP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23099">
        <property type="entry name" value="UTP20_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07539">
        <property type="entry name" value="UTP20_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 20 N-terminal" evidence="2">
        <location>
            <begin position="783"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 20" evidence="3">
        <location>
            <begin position="1552"/>
            <end position="1767"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 20 C-terminal" evidence="4">
        <location>
            <begin position="2137"/>
            <end position="2419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2401"/>
            <end position="2433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="2402"/>
            <end position="2417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW31357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="2433" mass="276932" checksum="2BC9E579EA3523DE" modified="2011-11-16" version="1">MPPVRKKTTESTKRHTFSSFRDRVDSIKIEPIRSLTKRPYDYTETSHFLTTLEHWKEINLSGNFTEFLDNVEIHSQSLPQIIHHQKLIYDCLHDSIKKNDVYSIQPLLELLSQFIHDLGPDFLVFYQQTLTLLTDLVLQVNPNDFQNNRNTSNVLEWAFNSLTFTFKYLSRYLTQDLKPTFTALLPILKLTKKTYISRFCAEALSFLIRKSNSESLKEIIELTLYDQDLTDPYCESLTTLFSESMKNTKGTFHSKSSLIFATLIEATLNHPSDNSAQPKLIGLICDVILDILHHGTPESCGKFYELVTKYLNELLTKNATTTASLTTSQILSTLAFAESGKKITNWNILLESVDNLISKLQGQVLSSELHESVTYLLVIVFRNSDVGEITKYHKKFLEFIFTTSYGLIFTQSSTTIAPKVLNFGVFKYVQDWIDRLEYDTTQLQQLAYFLEKSDQEFTLPGRIVSNIMEQLTGEDIYWKLLLLSHAKASLDEDTLLKLLELTQDDAEVAALVLQIATENKNKDFQSQVFTIIVDSFDKFYTSAGYIKALTQFVQQSKPQDTSSILTTALKCLYQPNHDTRSNAIDLIMTITESPYLSPISSIEATPLTIASSRDIIHNIRNLALDFQKNVKPTQLDKTIIVNYLFGLLSNKFQPCWNAVNEALPLISSSCKLEIWNLAYELLNVSGDEHSTNDQFLVESDLINWQPRNSRLFNNFESFDRDYLSPYRSINHSIAGLLNKDYSFSSILRPNILQALMTVPNVVDVSKLIPILINQPDEEEEVLWSLKDRNELLNLFTKLKDLRKSPQADDLREYLLNLLSSKNLRVQQLALDVLFTWNNPSINKYKDNLKNLLDETIFRDELSKFLARDSSSTIEDKDLSELMPFVVRILFGRVQGSPRSNSKQGKKFAIINVLPNLKDDDIVAFIQLGANKIGYKEFFEGVVPRASLGLEKSILNKINGFVNLLTQVYETLGSNFANVLQTTINPLVYSLVCAQNRIDTASEDVISDKIARNIRSNGMKCLYELFQLLGASFDWSEYVDVIYANLISPRMASFATENLQQPSSLLNVMTSWINNSNTWIFLNADEFTPTKAVLSLLGHNAKEAVLGKVLDFTVDALERKDNMDEQFFTLLALVVDSLLTNLSNIIEGITSKEIGSKAIKSLLLLISGNYMDDQETKSALIKALVVALNNNQIDLNDKANILISLAALIESYECTAEEILPLYETCSKLFRMFNERNLRETLVGVFIAIGNKFDKFAQVSQLLFGLNAYSTSRLQELDFEKRLEAFRLITEEIYLELDTTQWLPIIYNCLYFINDANELSIRINASYVLRRFIDCYSAKPAAEEAAPYIQILKNVILPNLKVGIRKDNEDIQSEYISVLEHLVHSNHYTELQDMQVLTYDNYDEANFFKNINHIQLHRRQRAIRRMIENASGLSENSIAHYILPMIERYAVSKDEKLMNVALEAMAAIKSLVVLINWNQYKAILKRYVSNLKDHESLKQRVNLIVVASGGLASSVKNQEGSLAQSLPNQEEIDDVILKDVSPAILKVLNVRDDDTIVARAPLAEALSNFTLCISKDKIDSELPKILTNTCQVMRSRSEELRDAIRKTLGKIATTLGPDYLPFIVKELKTALSRGSQIHVLSFSVHYLLQCIASSLIHGSLNEAIDLLMEIIMEDIFGSAGQEKDAEGYTSKMKEVKFKKSFDTVEIIAANISLSQFAHLLDPIKLILKEHVSYKTQLNLDELLRRLSLGLNHNEESSNLEILHLCYEIFTSSNPEDTKKKPVEPTEQEKHFLTNLERKSKVTHKDTSIYQHTMEKLSLELLRTAISRHDNLLTVGNLSGFVPLLDEAIRSENEAVVTSALKILNAIARLPFSEEQQNTFKSCARRSLIIIKESPSTNSDLCQAALKLLATTIRHTPEINLKQSAISYILTRIQPDLEEPQRQSIAFNFLKAVVSQHILIPEIYDLMDTVAKLMIVNHSQEIRAMSRSVYVQFLMEYDQGKGRLEKQFKYLVNNLTYPTEEGRQSVMELVHLIILKAGSDLLHKLASSFFVALSSVLVSDSSAKCREMAGSLISSIFKKLDNEGMANIEKYCTAWMKQNGNSLLKRCGFSVYRLFVTGFGIGKYETLDNIAHENILAVIESAKNSEDNDLQWELLYSALSVFSSIASAIKDKVLEKSYAKIWYALIDVLLFPHSWIRLISSRLISILLSNIDNLEFEVTNREIQMIAFRLIHQLRAPGITEELGLITSKNLVLIAMRWESEETDWEIEDNEDEEEETTSIKANDYLLGKISGIIRQETFQSLVAKKACIKLAAMFIQFTSEERLLQVAETIISSLYNFTDPNYASDEELTGLSLESLDLVKEKIGTSEYTRIYTKLKSQVNVRRMERKAKRAQMSVSVPEIAAKRKLKKHARSREKRKHEKDANGFYKSKKKRVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARW3</accession>
    <name>G3ARW3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thiamine thiazole synthase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Thiazole biosynthetic enzyme</fullName>
            <ecNumber evidence="6">2.4.2.60</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">THI4</name>
        <name evidence="8" type="ORF">SPAPADRAFT_51398</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[ADP-thiazole synthase]-L-cysteine + glycine + NAD(+) = [ADP-thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2-carboxylate + nicotinamide + 3 H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:55708"/>
            <dbReference type="Rhea" id="RHEA-COMP:14264"/>
            <dbReference type="Rhea" id="RHEA-COMP:14265"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:90873"/>
            <dbReference type="ChEBI" id="CHEBI:139151"/>
            <dbReference type="EC" id="2.4.2.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="6">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homooctamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">During the catalytic reaction, a sulfide is transferred from Cys-213 to a reaction intermediate, generating a dehydroalanine residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the THI4 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.60" evidence="6"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376158.1">
        <property type="nucleotide sequence ID" value="XM_007376096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARW3"/>
    <dbReference type="FunCoup" id="G3ARW3">
        <property type="interactions" value="886"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARW3"/>
    <dbReference type="GeneID" id="18871550"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51398"/>
    <dbReference type="eggNOG" id="KOG2960">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053727_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARW3"/>
    <dbReference type="OMA" id="MFPRIVV"/>
    <dbReference type="OrthoDB" id="410463at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160205">
        <property type="term" value="F:cysteine-dependent adenosine diphosphate thiazole synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03158">
        <property type="entry name" value="THI4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027495">
        <property type="entry name" value="Sti35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002922">
        <property type="entry name" value="Thi4_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00292">
        <property type="entry name" value="sulfide-dependent adenosine diphosphate thiazole synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422:SF3">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01946">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="256"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="105"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="316"/>
            <end position="318"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="2,3-didehydroalanine (Cys)" evidence="6">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="353" mass="37808" checksum="02402388EB96E1FF" modified="2011-11-16" version="1">MAPPTMLQNAQVATQFTLSTSGENTIRLTSDAENNADVKFANWDKFKFAPIRESTVSRAMTRRYFADLDKYTESDVVIVGAGSAGLSAAYVLAKNRPDLKIAIIEASVSPGGGCWLGGQLFSAMVLRKPANIFLDELDIAYDDEGDYVVVKHAALFMSTLMSKVLQFPNVKLFNATAVEDLITRRDETTGELRIAGVVTNWTLVALNHDTQSCMDPNTINCNIVLSTTGHDGPFGAFSAKRLESLGKAPKDVTQGFKPQSEQNTTATAPADGFQLGGMRGLDMNKAEDAIVKGTREVVPGLVIAGMELAEVDGSNRMGPTFGAMALSGVKAAEAVLNSIDHRQKQNQTGYGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARX7</accession>
    <name>G3ARX7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proteasome component ECM29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_51799</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376604.1">
        <property type="nucleotide sequence ID" value="XM_007376542.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3ARX7">
        <property type="interactions" value="1011"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARX7"/>
    <dbReference type="GeneID" id="18871595"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51799"/>
    <dbReference type="eggNOG" id="KOG0915">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000880_2_1_1"/>
    <dbReference type="InParanoid" id="G3ARX7"/>
    <dbReference type="OMA" id="CRIKDIE"/>
    <dbReference type="OrthoDB" id="16066at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042030">
        <property type="term" value="F:ATPase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024372">
        <property type="entry name" value="Ecm29_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055443">
        <property type="entry name" value="HEAT_ECM29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346:SF19">
        <property type="entry name" value="PROTEASOME ADAPTER AND SCAFFOLD PROTEIN ECM29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR GCN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23731">
        <property type="entry name" value="ARM_ECM29_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13001">
        <property type="entry name" value="ECM29_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24492">
        <property type="entry name" value="HEAT_ECM29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Proteasome component Ecm29 N-terminal" evidence="5">
        <location>
            <begin position="9"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome adapter and scaffold protein ECM29 HEAT-repeat" evidence="6">
        <location>
            <begin position="1269"/>
            <end position="1430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1809" mass="203052" checksum="A8B6AD34B265F841" modified="2011-11-16" version="1">MAEAELSLVNKAELRIALASTDEQFQHSLSLYLCPILLKLSSPHVQVRQAVLKIVQHLIPRITAARSITLPIDALVQQVKSPRVGPGVDAGSVQLYSTLFIFRGIDRITDEQRIKLVPDIIDNIHLYSDAIRARMFSIFSKLLTSWKAPFKGTDEYDAMRTTLGFDKNAEVEKYLSFTIAKFLLLLPDSNNKATPGLSLQDNSFFTSNAGVTYKTREEIFTTKRNLLEFLKAGFQNDQLALPLLVASADPSSSINESAEGLFKRLNIELDDVTIYGNVSLIDHLVLLFLGQPGIPAVDSNLQYKILRVLIKSTRIRLHPEISVISKTALHSDNPRIRQLAITFISWLGQSDNVNQELEQFNLQMVGELRNDIISEGWPSMELQGKVNFAQRIKDRQLKYETLGNILKSYPQIIDWSFIEFLYGSLEGDQPELRVSIQDALSGLSMHLSKLSQENKYLLKRLAAKYLTASADNVNISACRYIAIKHINSAFPFEDSETRFLSILGSSRENAPETIEEAQNGIHPYWFNIRQVNYTTGFKSTSYLLGQETSVIFPSFGAFMNVLTEEIDKAKSVQNSSIFKSLPTAILFAQQALVMESIKGNETVIIPDQDWAVRLDKAIEVNDKVRNLVTQHMENISIHEPIVQLLNLCIDALIGQSTHTVPTDTTYGTILSRIISFSSSPIVEFLIPRVAELVALLDKVHDEITISQICKAIGIIATHPSNVNSSIKELVVDLGLNNKTNILAVSYIISRLYLRDRVNIIDETTLVSHLDILEKMLQEQGSAYYSALDSISQLSMFGVLGPIQNQGLSNYVTTFKNIIKLKVKKIDEKSVLTLGYLILANNRDAIEGLSEDEQSIYDTHISKQIEYIFTSAESLTIAAAGWDSSVLSKQLDIPGEIVKYIPRDTSRAGIILDSVLGACKNTKPALRRAGCVWLLSLVQHCSKLEAITSRAGEIHVAFMKFLADRDELIQESASRGLGLVYELGDVELKDTLVKSLLKSFTSTTDSVKITAGTVESDTQLFEPDVLKTDDGSISTYKDVLNLAQEVGDPTLVYKFMSLAKSSSLWTSRKGIAYGLESILSQTSLDSLLAKDSSMSNRLIPKLFRYRYDPNEGVAKSMNDIWNSLIKDTSKAVTANLDPILAEVLKSMGRKEWRVRQAGTAALNDLLTMVEVEDYETKLDDIWTMSFRVMDDIKESVRKEATKLTKVLTTELTRLLESSSYKLKTEKISGILSILIPFFLGNKGVLSDSADVRNFSLEILSKLCKSNNKAIKSYVPELIENFILLFSSLEPEVINYLALNASKYNIDNNAIDASRLTSIGKSPLMEVTETLLSQLDDSNLPEFIIRLESAIKKSVGLPSKVCGSRVIVSLIMNHYQVIKPYGDKLLAVSSAQIKDRNDTIASSYAGSSGYLCRIASTNSILKYSEMIKKMYFESEDDRFRSISSVAADSVSKYAGDKFDLVSSAFLPLAYIGKFDAVEQVRENFDRVWIEHSGGKSAIKLYLAEIVDLIKKYIQSNKFEVRKTLARTLSDVSKSIDNLNEFSEPLIHDILSVLLEANKGKSWEGKELIFDALVSFAILSKSYISQHEDVHQAIEKTVTIEVKRRNKEYQKHVVLSVGKYIHEFHNEEVVDLYIQIMGKVLSDGYYQSDDDSDVEMDEVEPITVKENINLEESRLHLLQNLTEAFYIPVNDSLFTFVFNQIKEIFQSKIIANTWRSKVQTSEIYIAIIDILKDTKLKPNQVEFLLRTWNYLKLQSLLPESIENVKIQFIKLSYALIKYLTIDDAKVIKESLEEFAKNEPSSIVLTELRNALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS62</accession>
    <name>G3AS62_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UPF3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_52205</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RENT3 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377054.1">
        <property type="nucleotide sequence ID" value="XM_007376992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS62"/>
    <dbReference type="STRING" id="619300.G3AS62"/>
    <dbReference type="GeneID" id="18871641"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52205"/>
    <dbReference type="eggNOG" id="ENOG502SYE7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_769796_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS62"/>
    <dbReference type="OMA" id="EYHAMVE"/>
    <dbReference type="OrthoDB" id="18087at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12455">
        <property type="entry name" value="RRM_like_Smg4_UPF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039722">
        <property type="entry name" value="Upf3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005120">
        <property type="entry name" value="UPF3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13112:SF0">
        <property type="entry name" value="FI21285P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13112">
        <property type="entry name" value="UPF3 REGULATOR OF NONSENSE TRANSCRIPTS-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03467">
        <property type="entry name" value="Smg4_UPF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF3" evidence="6">
        <location>
            <begin position="53"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="202"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="16"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="207"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="232"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="278"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="289"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="313"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="360" mass="40602" checksum="CE284F8EABA20966" modified="2011-11-16" version="1">MASVATPRTAPPPSRPLTSNGNSGSSSSSNSNSAGTGTGTGTSSNHSTSISNSNLKLTIRLLPPTLTESEFLNQLASYYPYHATKIVHSYYHQGALPVKLFDLPEYSRSYLSFNNSNDVNEFLTYVKGKSFQDEREDCMIPLIEKSIYNKMIDSKVQEKNKNKRKKKEVNIEDDEIYLKFVAFVNNEIDSFQLKQVNKAVKKKNKKNAAEPAKKKKETKEEKAARKKLQRKLNKEKQKKKELEVKTGDKPKSEPNKPAKDKSKDKNTKPKDNKDNKPKPKPKKDKPKKKPEESKEPPKSAPESKEASTSEAAKRKRPRRKPKNKEGPKEEGNSQPKNKPKPKESQSEPKPKKPESRPVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS94</accession>
    <name>G3AS94_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar protein-sorting-associated protein 25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_156678</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS25 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377085.1">
        <property type="nucleotide sequence ID" value="XM_007377023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS94"/>
    <dbReference type="FunCoup" id="G3AS94">
        <property type="interactions" value="829"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AS94"/>
    <dbReference type="GeneID" id="18871055"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_156678"/>
    <dbReference type="eggNOG" id="KOG4068">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087657_0_1_1"/>
    <dbReference type="InParanoid" id="G3AS94"/>
    <dbReference type="OMA" id="TRCLIMW"/>
    <dbReference type="OrthoDB" id="245150at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.570">
        <property type="entry name" value="Winged helix' DNA-binding domain. Chain C. Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008570">
        <property type="entry name" value="ESCRT-II_cplx_Vps25-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014041">
        <property type="entry name" value="ESCRT-II_cplx_Vps25-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13149">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13149:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN-SORTING-ASSOCIATED PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05871">
        <property type="entry name" value="ESCRT-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="22491" checksum="CBEF0E3C302E7223" modified="2011-11-16" version="1">MMSDIPQFQFPKIHSFPPLYTKQPNTTILTQQLEAWTAIILEYCEYYKITRISKDGIPKHSQLEIDLDSLPPIFSNTAINRSVNSEFKTLIINSLIHKLNSGEYVNSKKPELGIFIYWRKLEDWGSLLYQYVDDSGQLGTVLTVYELTKSDEGGLPEAIRGLDETFLIKIIKGYLMKLGKAQILMNENNEIGGVKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASP9</accession>
    <name>G3ASP9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nucleolar protein 19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_142683</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377147.1">
        <property type="nucleotide sequence ID" value="XM_007377085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASP9"/>
    <dbReference type="FunCoup" id="G3ASP9">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASP9"/>
    <dbReference type="GeneID" id="18870473"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142683"/>
    <dbReference type="eggNOG" id="ENOG502S1GY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094334_2_0_1"/>
    <dbReference type="InParanoid" id="G3ASP9"/>
    <dbReference type="OMA" id="NNMYRIT"/>
    <dbReference type="OrthoDB" id="10597407at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022592">
        <property type="entry name" value="Nucleolar_19"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10863">
        <property type="entry name" value="NOP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="19271" checksum="ADB4E136BDB7BFDB" modified="2011-11-16" version="1">MSKNRRKEIQQKQSLQAQFQLALSQSEKRALSWLQPCRSTNPTPETNDDDSFMNLPVISQGAGLSSKNGSSSTIGEFLSSDLKSTKQQQQVKEMRKPTSKPMMALMNKMRNTSRETVRKKVERVKQEVKQADNNDDDSDDEEISQLRARSVKKTTGGILQHKIKKSGRPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASS7</accession>
    <name>G3ASS7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calcineurin subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Calcineurin regulatory subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Protein phosphatase 2B regulatory subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_63056</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Composed of a catalytic subunit (A) and a regulatory subunit (B).</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the calcineurin regulatory subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377174.1">
        <property type="nucleotide sequence ID" value="XM_007377112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASS7"/>
    <dbReference type="FunCoup" id="G3ASS7">
        <property type="interactions" value="851"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASS7"/>
    <dbReference type="GeneID" id="18874574"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63056"/>
    <dbReference type="eggNOG" id="KOG0034">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061288_10_4_1"/>
    <dbReference type="InParanoid" id="G3ASS7"/>
    <dbReference type="OMA" id="KXAFRIY"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000001">
        <property type="entry name" value="Calmodulin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45942">
        <property type="entry name" value="PROTEIN PHOSPATASE 3 REGULATORY SUBUNIT B ALPHA ISOFORM TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="22"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="54"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="91"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW31141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="130" mass="14495" checksum="4C789AE312B0F3FC" modified="2011-11-16" version="1" fragment="single">MGQGPSSGIISGLMADTNFNPEEIDRLRKRFMKLDQDRSGEIDKQEFLSIPGVSSNPLAARLMDVFDIDGNGKIDFQEFITGLSAFSGKSSTEEKLRFAFKIYDIDRDGFIGNGELFIVMKMMVGNNLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASY5</accession>
    <name>G3ASY5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Non-histone chromosomal protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">NHP6</name>
        <name evidence="11" type="ORF">SPAPADRAFT_62621</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NHP6 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376800.1">
        <property type="nucleotide sequence ID" value="XM_007376738.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASY5"/>
    <dbReference type="FunCoup" id="G3ASY5">
        <property type="interactions" value="445"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASY5"/>
    <dbReference type="GeneID" id="18874409"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62621"/>
    <dbReference type="eggNOG" id="KOG0381">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082854_10_3_1"/>
    <dbReference type="InParanoid" id="G3ASY5"/>
    <dbReference type="OMA" id="MKNMGGK"/>
    <dbReference type="OrthoDB" id="1919336at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01390">
        <property type="entry name" value="HMG-box_NHP6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000016">
        <property type="entry name" value="FACT complex subunit SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF22">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION FACTOR A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="10">
        <location>
            <begin position="20"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="8">
        <location>
            <begin position="20"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="93" mass="10594" checksum="CAB2D78469947769" modified="2011-11-16" version="1">MAAAAEKRKATRKKKDPDAPKRSLSAYMFFANENRDIVRAENPGISFGQVGKLLGEKWKALTADEKVPYENKAETDKKRYEKEKAEYAKRNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT25</accession>
    <name>G3AT25_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin-like fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_142882</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376821.1">
        <property type="nucleotide sequence ID" value="XM_007376759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT25"/>
    <dbReference type="STRING" id="619300.G3AT25"/>
    <dbReference type="GeneID" id="18870497"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142882"/>
    <dbReference type="eggNOG" id="ENOG502S0CB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085801_0_0_1"/>
    <dbReference type="InParanoid" id="G3AT25"/>
    <dbReference type="OMA" id="IKFSRQN"/>
    <dbReference type="OrthoDB" id="37297at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33875:SF2">
        <property type="entry name" value="ACR183CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33875">
        <property type="entry name" value="OS09G0542200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="24918" checksum="83059F4FA2B37308" modified="2011-11-16" version="1">MISPKYSLTHYYLSAKTLAASQGVSPHIINLYLDYNCPFSAKLYLKLYNKVIPELQAKHPGKFQFVYVNVIQPWHTNSNLLNEFAIAYAKLLREQPSGDTNGDFWKLSEVLFKNKESFYDTSNIDLTRNQIYAQIYDVVSKELKLAFDKKTILSQLEIPHSETPSNAGNATAVDVKYFTRYLRGVGVHITPTVSINGIVNDAISSGTPEEELIELFEKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT33</accession>
    <name>G3AT33_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Oligopeptide transporter protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_142923</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376829.1">
        <property type="nucleotide sequence ID" value="XM_007376767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT33"/>
    <dbReference type="FunCoup" id="G3AT33">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="GeneID" id="18870502"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142923"/>
    <dbReference type="eggNOG" id="KOG2262">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004965_1_0_1"/>
    <dbReference type="InParanoid" id="G3AT33"/>
    <dbReference type="OMA" id="TETPIWG"/>
    <dbReference type="OrthoDB" id="9986677at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00727">
        <property type="entry name" value="ISP4_OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="289"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="357"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="435"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="504"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="577"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="604"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="635"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="754"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="801"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="830"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="899" mass="102902" checksum="C18048E5FE7805B1" modified="2011-11-16" version="1">MSKGYEYIELTPYLESPSWVTSTNSNPQRWNFTESQLQLILLRLGVISDEIYGTDFALSQAHLSEETKYMITKINELSISDAIEIVRDALAEHSGDVNFLREDYQLLERLVSMIPANPEAGEPKHESSENFVNVFKNKTYLNVIDWSLQIRLEAALIHFHSPYPEIRAITEPFDDPEIPVETFRVYFIGLFWTFIGSIINVFFVHRMPSISLSSHTIQILLLPSGKLWEKFVPNKTIRIGKYSIDLNPGPWTYKEMMLSTIIYSCSAGTPYSVYNIVVMKLDRFYGLKWVTYTFQFLLAISTQFLGFGFALIMKKVCVYPSKALWPTILPTIALNKALMNEDENNVVHGWSISRYSFFMVVFFFSFVYNWIPTYFFTALSLFNWPTWFNPSSIHLTNVAGSINGLGLNPLPSFDWNILGSAGCLTVPFYTYANRYLGSLMGFVVILIVYYTNNNWTGYLPINSNRLFNNKAEVYDVHKILDEKNLFDDAKYQEVGPPYFSAANLVLYGAYFCLYPFAILYHFMTEWESMKSGFVNVWHSLVEAFKFRKDNSHENIYGRYAEDPHCKMMSKYEDVPDWWFVAILVISTVFALAVVLFYPTETPIWGIFFTILINFVFLIPLTSIASVTGFTFGLNVLVELIVGYAIPNSGIALITLKSFGYNIDSQASNYITDQKLAHYAKIPPKAIFKGQLISTMLNVVVALVVTNWQLANVSDICSPHQKDKLKCPGANTYFFSSIQYGEIGPAKVFSGLYPILKWCFLLGTLLVFPCVWFKNNGPKKLTRYFQPTVIIGGFLDYAPYNLSYLTGGLYLSYIFMYKIKKNYLLWWEKYNYILTSALSAGVAFSALLIFFTVQFHPHHLEWWGNTIGEQGIEGGALPDTWLNVSQAPDGYIGLRKGQFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATB8</accession>
    <name>G3ATB8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_142526</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376914.1">
        <property type="nucleotide sequence ID" value="XM_007376852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATB8"/>
    <dbReference type="STRING" id="619300.G3ATB8"/>
    <dbReference type="GeneID" id="18870457"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142526"/>
    <dbReference type="eggNOG" id="ENOG502S1WJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_478188_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATB8"/>
    <dbReference type="OMA" id="RLWKWDY"/>
    <dbReference type="OrthoDB" id="4703at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052416">
        <property type="entry name" value="GTF3C_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15052:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15052">
        <property type="entry name" value="RNA POLYMERASE III TRANSCRIPTION INITIATION FACTOR COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="543" mass="61111" checksum="F7CB35A7B8B1739D" modified="2011-11-16" version="1">MVKVTLSKFSRDQSFKGRFAHHYGYNSEYFDVLCKYKDDWSDKLFFVNEQMAKKYVELAKPIVTIMEEELNLAELSVTEMTSDDIQLLFTNDIPMKITYGKETHEVNLTTMDNTVDLTKLVKRNALILSPGGHITSLKWLPQVLKQDTPKVNYLAVSIINNPNGVGDSILNPELSMYDGNTTDHKINSAIQIWKHDLNTNELTLEHMLVTTNLGATCDLTWCPMYLEEGNVLGVLAASFTDGKLHLFRITKNLPQYAKVDQTSLSYVSQQSSEPQKSDPCTNIISFEFLGIDKILVGTTDGSIAEYILPFKRQEENIDDISIPSYKTKISDEPITSIVAAEYGTGEYIILINTISPIVFSYDYNNYIQDFHTPISRSTLPPSYNHVLRTFTVTPTVDSVGYIFLRSPQETMHSLLKLDSYLTSNHASEILGHPLNLSGTMSGQIIVLNYSRKILNGQKTTNRVLVPLQLWKFDEDEDKPGTVVLQADFGRMVVDTPVEMSIMPPELAISALAWNENVHGSSIYAAGTVSGVLIIERLDPKYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATH0</accession>
    <name>G3ATH0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein PMU1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">PMU1</name>
        <name evidence="1" type="ORF">SPAPADRAFT_56876</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376966.1">
        <property type="nucleotide sequence ID" value="XM_007376904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATH0"/>
    <dbReference type="FunCoup" id="G3ATH0">
        <property type="interactions" value="174"/>
    </dbReference>
    <dbReference type="GeneID" id="18872075"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56876"/>
    <dbReference type="eggNOG" id="KOG4754">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039184_0_2_1"/>
    <dbReference type="InParanoid" id="G3ATH0"/>
    <dbReference type="OMA" id="YPWDFEV"/>
    <dbReference type="OrthoDB" id="496981at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF1">
        <property type="entry name" value="HISTIDINE PHOSPHATASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="330" mass="38455" checksum="C4A347E970360ADE" modified="2011-11-16" version="1">MTKQSLLIPTQDDYDDAHGNVEDEVKYHDQYKTARNDKDENGNWIYPWSFEVVPGFFKQCDPKTDDLKFNYSFENMGRLKPWDEMISELQALNENANDNEVYKLVFLARHGQGYHNVIVQKYGVAAWNEKYHAMTTDGELTWAPDPQLTETGIKQAQENNQLWKQEIRLGAPIPSKFFVSPMQRSSNTCVITWDDIKPKEQRPVVKEKLRETCGKNLCDKRSSKSVIDERFGKYGFDTHEIVEEDIYYTEDRETMVHHSFRVNQFLQELFEQDWENGSVDKTKAIENTFISTTSHAGTIRCFITVLKHRNFTISTGGMIPIVIKATRRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATI8</accession>
    <name>G3ATI8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="6">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_52142</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="6">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="6"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376984.1">
        <property type="nucleotide sequence ID" value="XM_007376922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATI8"/>
    <dbReference type="STRING" id="619300.G3ATI8"/>
    <dbReference type="GeneID" id="18871632"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52142"/>
    <dbReference type="eggNOG" id="KOG0342">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_21_0_1"/>
    <dbReference type="InParanoid" id="G3ATI8"/>
    <dbReference type="OMA" id="AHEKIDQ"/>
    <dbReference type="OrthoDB" id="193716at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="89"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="303"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="564"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="615"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="632" mass="70340" checksum="054EA70932417B85" modified="2011-11-16" version="1">MSLSIMISLRQLSRSFATSARPVATCSLRSRGLHTSTVHFNETPSTTIDPSTIPKTPADLEGKVSASIIESLKRNNFNTLTPIQQLSLLPLLKTEKGMVCRAKTGTGKTLTFIIPTLESAIARKTPGVDTLVVAPTRDLAQQIFEEYLKVISKLPAKVRKPRVALITGGRSGRINLRDPEAIVIATPGRLAADLDNRSAAELFDSLSFRVYDEADRLLDIGFEPTLDSIHDSIMRVRANPDKPLKSLLFSATIDDCMTNFAVKHINPKYDYINTVADDEPNVHENIHQILVECEDAIDKYQTAISYIADAVQEEHFRGIVFLPTKTSVDWLEDYLKQISQAGIASERRLFGHKGEAQLLKIHGGMTVSRRENALRMFKNCPKGLLIATDVVARGIDVKEVTHVFQFGVSDDPLDYVHKVGRTGRAGKTGKAVTFLTTSERPYTNTLARRAQVSFKEMIKSSEIHQYDIYKVQADYEETKEFMLPNFSFLIQTCDKYRLNLNKLVEENMEFFRRVTGEADAKIRLSGFMKGSLKRVSRDISDEYFVGGGGGSAGGYRDGGYSRGGGYNSGRQGSYGNYTRSDRGGYNNRGGDRGYNNRGGYNRGDRGSGNRGGYKKGYERSSRNRDDFDDLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATJ5</accession>
    <name>G3ATJ5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62865</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376991.1">
        <property type="nucleotide sequence ID" value="XM_007376929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATJ5"/>
    <dbReference type="GeneID" id="18874497"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62865"/>
    <dbReference type="eggNOG" id="ENOG502T1UR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_636194_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATJ5"/>
    <dbReference type="OMA" id="YRYEYYY"/>
    <dbReference type="OrthoDB" id="4097096at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="429"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="330"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="365"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="429"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="462"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="489" mass="54081" checksum="132CD419D100CC4A" modified="2011-11-16" version="1">MEMANTLMRSSSMSNGTTQTSRTSSSTIMADTSSSKSSITTCTPVPTKPYNNINSYPVSVVPTPSTTPVQACPPLDELVNDCFLDSIGQLEDPVKVAFVKKFNDYLIKQHCQENLHFLIDIFNYELYYNKIFPDSNTYNADKERLNSAPNASLLGYITSNTSSVDSLLKVKRDSISTTISKRYSITSTDDLDPQEMFVSTIDDLDANEYAQFNVNIWEEFGQRQIEDEDDEDSSQYVGDDATFTEDEEVEALNSRWHYIMHTYIKHESIQQINISQKLFKEIVEESSHSGVHNPLTLIKARNETLRMLKENGYLSFVSHCKRERASSNQPSPTVSTLPNSPSSLSLEGSPGNTIAKPKSIPRATSPLQSSINNSNSSLSHSSSLISKLSSLPKRNRGPLFSSASSDEYQAPTPSSINNILSHLKLSETTTTNNNSVASTSSRNVDSASSSKRQSRSTTPKISSSNGTNSPSSGKEDSSSLSFKFWSRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATN4</accession>
    <name>G3ATN4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Superkiller protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_142101</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377030.1">
        <property type="nucleotide sequence ID" value="XM_007376968.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3ATN4">
        <property type="interactions" value="532"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATN4"/>
    <dbReference type="GeneID" id="18870422"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142101"/>
    <dbReference type="eggNOG" id="KOG1127">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001688_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATN4"/>
    <dbReference type="OMA" id="CQWELDP"/>
    <dbReference type="OrthoDB" id="421075at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055087">
        <property type="term" value="C:Ski complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070478">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039226">
        <property type="entry name" value="Ski3/TTC37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040962">
        <property type="entry name" value="TPR_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15704">
        <property type="entry name" value="SUPERKILLER 3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15704:SF7">
        <property type="entry name" value="SUPERKILLER COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18833">
        <property type="entry name" value="TPR_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="38"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="479"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="709"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="994"/>
            <end position="1027"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1404" mass="160726" checksum="7FC06C64FAA5C4F0" modified="2011-11-16" version="1">MSSLKKLLKSAKLSIEQNDPESALEYADEAIEIDSNCYFAYLFKGKSYQLLRDVPQAIAQFKRATEIEPENLLAWKGYFQTVRASNDYSLFFDVLTKLVKLQRDQELPLAETLKDLRNYLDSNKYKSNDVLYELYLKSIIPGTELGELVGSALGSGESNIQKLLDFKKTQLDKLVQTRISKERVKFGRNLTIDQKQALDAIAWEYYKDGQLSKLYESFLNISNDDELRQKYEEDFLKYRYELLKISPNKTELFNEIKQTLEDMILVNTKSLFCWNLYFDWCDTKTIFDLDEEKIIAYLRIFQNEGLGLILFAFVMSGVSPYNKERIVKGISYNEDNQNKKNKKKKKEKVLEEEDASALGLEENEEEQPEDFSQYYLPPDEILGYILEGYSKSKDSILANRIIVNYYIHLREYDMASERCRDGIRLLADIQRTFGINLSNTKEDFLCSLAIVYTYYEAPKNFSRALQLYDKILESNTTNIKAKVGKGLIFAEQGDLEQAKLILEEVLQEHPENSEAELEYNWCLINLGQFEQGKQGLEKFISKVTGTDLYSGEIRAKANWRLAKGYFMQDDTNKDNIKQSYTYLIQSLKDSESYAPSYTLLGVLFHDYYGDLEKAQKCFFKAFDLDVNEIVAAKYLVEHATAKNEWDVAQVLAKRVVTNESSRRLLLRPSDDKDNSWPYRVLGCGSLNVQDDAKAVEYFQNALRINSNDYECWVGLGEAYYNCGRYDAAAKVFHHAIAMKEEAPWTVKYLLGVVTCEMKEFNEGLTYLYEALESQPNEECIITAIYEAQLENTHKYVHSGFFGRAADSSLKALEFIKASILSNSTSQKVWKSLGDSLQVFSKVQSNIDKVPFDDLIAIFKHIEIDDIILSDITLDESISLAHAIELFNQDKKVQSLNIFIILCAIAGVKYLPVKVNKALRSTALYNLGLALLQTYQNGENEKYRENSVTLFKKAIQLENHNPSYWIGLGNAYFYNNPQISQHCYIKATTLEIRDGGIWINLAILFLKYGDLELSQQAFLRAQSVAPQDSQSWLGQAIAFEILGDETKAFTYYTHAFTLSKGRLAVAQFLYGLSVINKRVGGNFDPRDIETAQEFGISNQAMQQYLKYKPDDEDALSVALTIAERCKDFENAIKLGNKLCEILEKKYEETEQESVLENFSLIKSQVARIYLGLGDYESAMENAQMALQLIEGDDSHPEITLSCHITIGLSYFFTNEFESSIQELKLILSQYNSSQRIVTLIAQILYAHGTEESKQASIDQLFAHIEENGSSLLVVLTLGAISLVENYQEYLGAIKEELQGLDLTELVNDTYRDVPRLLTEINNRLGRGDDKSVWLKYAYLFPFDYNVWKHVSNEMAKAVITLQDVKVNGLQYSETLLKTGDLRDIQRSLMLTPGNQEAIEALKNCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATY2</accession>
    <name>G3ATY2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_63211</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377329.1">
        <property type="nucleotide sequence ID" value="XM_007377267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATY2"/>
    <dbReference type="FunCoup" id="G3ATY2">
        <property type="interactions" value="278"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATY2"/>
    <dbReference type="GeneID" id="18874643"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63211"/>
    <dbReference type="eggNOG" id="KOG1505">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041844_3_2_1"/>
    <dbReference type="InParanoid" id="G3ATY2"/>
    <dbReference type="OMA" id="RMVMIAN"/>
    <dbReference type="OrthoDB" id="189226at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036149">
        <property type="term" value="P:phosphatidylinositol acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07990">
        <property type="entry name" value="LPLAT_LCLAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032098">
        <property type="entry name" value="Acyltransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983:SF16">
        <property type="entry name" value="LYSOCARDIOLIPIN ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16076">
        <property type="entry name" value="Acyltransf_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="380"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="5">
        <location>
            <begin position="103"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="404" mass="46953" checksum="2DD4C74CD3B41FC5" modified="2011-11-16" version="1">MSLRHTWPVIIVRSAFLVPTFLIGCLFIVFTQVVGLYVFQHNAPSRQAMINITKTHFVILITFMTSLINPTKIAITLDKSVPNPQQFTVDNGGHLHTSFKPNSIFISNHQIYTDWLFLWFLNYTSRLSDYIYIVLKDLSSIPVLGYGMKNYNFLFLSRKWEKDKIKLTNQLLEIDANARGVGPANGVTLVSTTNTNKIREWPKTNQPSKIWPYQLILFPEGTVPSDRTTKKSREYIQANGHEPMKHVLLPRIRGLYLALKKLQNTLEVVYDITIAYSNLKETEYGEDVFSLKRYYLKGYGPERINYYVRCFSIKDIPLGDEEDVDDISPEALKKFEQWLLNVWYEKDELMSNFYKLGHWDKPNDPTVVGDFKLRNQIEIVLPYLSGITALLLLRLVYKLIRGMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATZ4</accession>
    <name>G3ATZ4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_68476</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377341.1">
        <property type="nucleotide sequence ID" value="XM_007377279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATZ4"/>
    <dbReference type="GeneID" id="18875220"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68476"/>
    <dbReference type="eggNOG" id="ENOG502T68I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_845111_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATZ4"/>
    <dbReference type="OrthoDB" id="4020980at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="329" mass="38205" checksum="B661DD009C20DBF3" modified="2011-11-16" version="1">MSLYQEYQGDNPPRERITRPVCPHPRPPLSPIPSKPNFKCNLRLPDDPSPIPRTTPIGFLIDDASMDTSYPGGDNESILSEARSFLTPDLKDGCESMNRIASPSDLTFYSYLSQLKSTLPLLTHTVSPCNVLAQFFANEIYHANNLITICYSFCNTEFHSFPHTSILNPYIPQDKTHHSRMIHQLKKLQEITRELISRLAMFNSLIDKVNLFDVSFTNLLSMRAQFTYLINNFVIEEIEEISKKLQRELYKYNLFVENIIESDDNYVEQEDDISFATIENLRFKIEQTLLFKTNKLTKVDLQHFYEVLITGVIEDAAWWNYIENLRDMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU54</accession>
    <name>G3AU54_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Lysophospholipase</fullName>
            <ecNumber evidence="2 10">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_157445</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the release of fatty acids from lysophospholipids. Phospholipase B may well contribute to pathogenicity by abetting the fungus in damaging and traversing host cell membranes, processes which likely increase the rapidity of disseminated infection.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the lysophospholipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="2 10"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377401.1">
        <property type="nucleotide sequence ID" value="XM_007377339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU54"/>
    <dbReference type="STRING" id="619300.G3AU54"/>
    <dbReference type="GeneID" id="18871081"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157445"/>
    <dbReference type="eggNOG" id="KOG1325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014602_0_1_1"/>
    <dbReference type="InParanoid" id="G3AU54"/>
    <dbReference type="OMA" id="GPEQYSL"/>
    <dbReference type="OrthoDB" id="4084751at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000010">
        <property type="entry name" value="Lysophospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF33">
        <property type="entry name" value="LYSOPHOSPHOLIPASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9 10" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6 9 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013356815" description="Lysophospholipase" evidence="11">
        <location>
            <begin position="16"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="PLA2c" evidence="12">
        <location>
            <begin position="28"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW30430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="600" mass="66704" checksum="CA63346927DDC054" modified="2011-11-16" version="1">MNLILYLTLLLPVFATSPTNDYTPGKVKCPGTKLTRLATAINSKEVGYISNRGKFSKLHLTNFLHNANMSDFDIDKFLETNKPIIGLAFSGGGYRAMLSGAGQLAALDSRVTSPDRTLAGLLQSSSYITGLSGGSWLIGSLACIDFPSVEQVMRDNILWNFEHSILDYYGLNVAANKLMWAMISLDVLGKQMAGFATSITDPWGRALSYQLLSTLKDNGDAMLWSDITNSSSFKNFSMPFPIISAVCREHGTYIRSINSTVIEFTPYEMGSWDPSLNSFVDIKYLGTNLDDGIPKEGTCMSGYDNAGFIMGTSSSVFNEAITELDQSGLPSFLIELIKKYILTPIERHNGDVANYHPNPFYNSKNQKTRLANSDTLLLVDGAEDGQNIPLVPLLNRNVDVIFAHDNSANTDGWPDGRSLIATYERQFSLQGENLAFPYVPDQSTFRNLNLTSKPAFFGCDAQNLTTLTDDIYGVPVVVYLPNRPFSYWSNTSTFKLVYSENERRSMIRNGYEVSTRKNGTLDSEWAACMGCAIIRRSQERNGQEQSEQCKRCFKRYCWDGTIYSGPDLGDNFSDEGLTKSDQYYNKKNTPGFNNGATKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU65</accession>
    <name>G3AU65_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_143763</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377412.1">
        <property type="nucleotide sequence ID" value="XM_007377350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU65"/>
    <dbReference type="FunCoup" id="G3AU65">
        <property type="interactions" value="124"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU65"/>
    <dbReference type="GeneID" id="18870573"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143763"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017390_1_1_1"/>
    <dbReference type="InParanoid" id="G3AU65"/>
    <dbReference type="OMA" id="YGRDRCA"/>
    <dbReference type="OrthoDB" id="6407164at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051252">
        <property type="term" value="P:regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000047">
        <property type="entry name" value="Differentiation 1 negative regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000064">
        <property type="entry name" value="Differentiation 1 negative regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000120">
        <property type="entry name" value="Negative regulator of differentiation 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF8">
        <property type="entry name" value="RNA-BINDING PROTEIN MRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="171"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="262"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="400"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="491"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="103"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="572"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="103"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
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        <location>
            <begin position="153"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="572"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="593"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="612"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="629" mass="70312" checksum="95A5D6D008FBF11D" modified="2011-11-16" version="1">MSYYDPYQGLPPQQYGQPPYYPQYYPQQDDQSIVNGQENIQQQQQQPTMEPPVASYDQYPQYQPESQYNFVNGSGSSDVTIGNEFYNDDGVYVSNQVVNSGNGNGYINQVVPPQQQQQQQQQQQQQQQNQGQFNQGIQSHNQSIVGNSVPTTQGQQEMFQQQESSSVAPSRTVYLGNIPSDIQPNELLDYVRSGILESVRILPQRNCAFISFVDHQSALLFHSDCILKKLNIKGHDIRIGWGKNGPILPAVLEAIQRDGATRNVYLGKLNNPISGEVISEEELREDLSCYGVIDCIKIIPEKGIAFVHFLSILSAIRCVANLPLKEKYSDKKCFYGKDRCAFITKTQQHNAAQYLGLAPGMEHIVTAADREFISSALVQQSAAAAQIATQAGGANNLGNRTVYLGNLHQDSSVEEICNVVRGGLLQSIRFLKERHVCFITFIDPIAAAQFFAMCQLHGLTIHNRRIKVGWGKHSGPLSNALSLAVSNGASRNIYIGNINDFNYYNPQKLRQDFSKFGDIEQINYLEEKNCAFINFVNIANAIKAIDGIKSFEDYKVLKINFGKDRCGNLPRQFQNQSSNQLGSLENGSSQSFSDHEFGNGNNVHERSTSLEPTTSLQQSFTQPQQQMDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU95</accession>
    <name>G3AU95_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CP-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_143899</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in the mitochondrial lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_007377380.1"/>
    </dbReference>
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        <property type="interactions" value="1275"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU95"/>
    <dbReference type="GeneID" id="18870582"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143899"/>
    <dbReference type="eggNOG" id="KOG1424">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011072_2_0_1"/>
    <dbReference type="InParanoid" id="G3AU95"/>
    <dbReference type="OMA" id="VNKADMM"/>
    <dbReference type="OrthoDB" id="61815at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01857">
        <property type="entry name" value="HSR1_MMR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001151">
        <property type="entry name" value="Large subunit GTPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043358">
        <property type="entry name" value="GNL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709:SF2">
        <property type="entry name" value="LARGE SUBUNIT GTPASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709">
        <property type="entry name" value="LARGE SUBUNIT GTPASE 1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="8">
        <location>
            <begin position="193"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
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        <location>
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            <end position="313"/>
        </location>
    </feature>
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        <location>
            <begin position="627"/>
            <end position="658"/>
        </location>
    </feature>
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            <begin position="18"/>
            <end position="32"/>
        </location>
    </feature>
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        <location>
            <begin position="304"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="639"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW30471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="658" mass="75552" checksum="9769D272675A0471" modified="2011-11-16" version="1">MSEFGSNKGALHPEQKKWKAPKGPKPVHHKNKNMIGLGRSIANQRRQENQIEFLPDGEMRFTTDKKEADWVKLRSVTQENSLDEFLNTATLADTDFTAEKGQQVRIIKVGNTNIVNQSGLLTNDEMLAVRQRHMVFENKLTIPRRPRWSKEQSKLQIERQENLAFLEWRRELAALTENNDLLLTPFERNLEVWKQLWRVVERCDLVVQIVDARSPLFFRSIDLEKYVHELSDPENNKTKKNLLLVNKADLLTRDQRIAWADYFHAKNINYVFFSAAKANELLEKEKEELENLRLDPNYQPQSKDDDDEEEEIVETNDSIRILKIEELEELFMSEAPEFEIDPDFPDRKLQIGLVGYPNVGKSSTINALVGSKKVSVSSTPGKTKHFQTIHLSPDVLLCDCPGLVFPNFAYTNGELVCNGVLPIDQLREHIPSVSLVCQRIPKFFLEAVYGIHIPIQKVEDGGNGEYPTARELLNAYARARGYMTQGFGSADEPRASRYILKDYVNGKLLYVNPPPKKQDDEWTLPKLEECRDFNKELYTLNHLPESRQQQIIAAMMKKNISVDEFDLAQDLAKLNFSMHIGKDEKTNDTGSPKTLFYGGKQAALESAGDELDREFFKMNGVEGKFVTPFHKKESGDGKGKKHNKKNKKQDKKIRVVGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUE6</accession>
    <name>G3AUE6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_52600</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377493.1">
        <property type="nucleotide sequence ID" value="XM_007377431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUE6"/>
    <dbReference type="FunCoup" id="G3AUE6">
        <property type="interactions" value="335"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUE6"/>
    <dbReference type="GeneID" id="18871699"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52600"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012324_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUE6"/>
    <dbReference type="OMA" id="NKITEMH"/>
    <dbReference type="OrthoDB" id="10044938at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051186">
        <property type="entry name" value="RRM_HNRPC/RALY_subfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968:SF26">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="350"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="416"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="559"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="707"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="17"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="45"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="146"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="440"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="610"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="636"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="669"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="712"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="734"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="753"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="764"/>
            <end position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="801" mass="89587" checksum="E73C6B9277068B48" modified="2011-11-16" version="1">MEVKPISLHRPNDEDLNSNSNGTTVSSSSTESVSEHVEEQQQGEQEAEGVAEGEAEGQQEEQQEEEEQEQEQEQQEGEEKSAPGDEKESKDAIQEQEEGAAPDQDDEDEAGVEVELHNDVDYDPATALSSDDSKFVPSEAPNNGESNDEEDEDEDDYDPELALEQSPLPTHQDTNKEPPKSQTLPPPPPPPSNLKGKPAPVGLPPKPPVMAGSHKKVVSPTPATTQIPDPQHKLKEAYDAVMHSEIAKDPNFVNLPQDEQMKLIIDQLNKQGVSLTSSNKAMNYDQVYSYNKPFKNLKDPIPLVPVNEYCRRPNITAPMSREEEHAYEEFIKQESYYMNLQNWDEFPDKSRLFIGNLPANTISKQDLFRIFSQYGDVIQIAIKAGYGFVQFRTAEACLECIKGETNVPLHNKILRLDASKPQKSRRPGRPEINNPNLSSRGRERVREDELSPQNKRRKMNVDCMVYITGKSSVFFVRQVKKAFSNCQVMIDTEDITHRNLSDVISEAAYSGVLGACVVKEFKVDVQTFESTPDGGIKFDEYADVDPEVGADLIAKAKNKRYGGNPPTYYPQDVSYNAPPEPAPYQQPQPYGVDPYYNEPMHSNHYDRDRKRGKRGGRDRKHRDRGRSGYSNQGWDQQPQSQPYGSSSSPYNQYNQPVPSQPQFQPPTSQPYRFAQQASAPAPAPTQSDQAGLMSALQGLNPSQVQGMISLLQQQQQQQQQQQQQTPPQQHYNQGGYGGHGGQYSNNYGGGNQVNALLSQLQNRQGSSYSSQQPQQSQQQQQQQGGSSTQSLMETLARLARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUG7</accession>
    <name>G3AUG7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_143676</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377224.1">
        <property type="nucleotide sequence ID" value="XM_007377162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUG7"/>
    <dbReference type="STRING" id="619300.G3AUG7"/>
    <dbReference type="GeneID" id="18870563"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143676"/>
    <dbReference type="eggNOG" id="KOG3030">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065408_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUG7"/>
    <dbReference type="OMA" id="WYHAPAG"/>
    <dbReference type="OrthoDB" id="8907274at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03390">
        <property type="entry name" value="PAP2_containing_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF192">
        <property type="entry name" value="PHOSPHATIDIC ACID PHOSPHATASE TYPE 2_HALOPEROXIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="7">
        <location>
            <begin position="110"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="352" mass="39910" checksum="5EED65ADDE8AC0A2" modified="2011-11-16" version="1">MHLFTRDWPIKLRTITLSGLTVLSYTFDILFYSTLFTLSAALGTIIPPRFHEFSLFDISLRYTYRTEAESAVPIPLLVFISGGIPIIQFIFFAIFQPRLSISRRLWDLMSGFMCLLGAMATQLLATCILKNICGLPRPDMIDRCEPMFQNIPVTQLSNVAICTQPNWNLVQEGFRSFPSGHSSAVFCGMTITSLNIASRLQTFDSRNNSFKVFLTILPIFLAMFVACTRVSDNRHFLRDVIGGSLIGTYVGSLFYWQYFPSIYNLSNNGRAYPPRRIGVHPFFNNIGGFWKIPDRLKGAFEQRVLNGPEGEAHLEKLQNGYLNQTVAPTTIQDNITLVNRLNEVLPEDIAEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUK4</accession>
    <name>G3AUK4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_63397</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377531.1">
        <property type="nucleotide sequence ID" value="XM_007377469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUK4"/>
    <dbReference type="STRING" id="619300.G3AUK4"/>
    <dbReference type="GeneID" id="18874729"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63397"/>
    <dbReference type="eggNOG" id="ENOG502QU9H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_862107_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUK4"/>
    <dbReference type="OrthoDB" id="6347512at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="323" mass="37114" checksum="2F438A5FDBE98AE6" modified="2011-11-16" version="1" fragment="single">MNQEEYDQFFALLVNILENSGGTIQLPSVKHFVSYPQEPYFKSLGYRSKLIKYTTKLQAWELVDIIERDISYLKQKSNEDDSIVKEYLTGILDLFKLKQEQSIHETLNQCIPKLFDLILLANCKDSLTYKLVQYLQSLPSSVINQLTETAVLPPPTSVYSMLLDGDIVYLSSICNHIANSRKFKYKNPELKQLQNSYIMDTVNFLWRDKFMHSEAKSANRGMYLPATLVDKLSSHYDIPQPATLGNIFMNPALSYIVTRIVWKLEDEQEVGIRHAGPISRQSVIELNESDWLNMSYDDLKVKIIEKLDGIASDGVCELLYTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUL7</accession>
    <name>G3AUL7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_52646</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377544.1">
        <property type="nucleotide sequence ID" value="XM_007377482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUL7"/>
    <dbReference type="GeneID" id="18871701"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52646"/>
    <dbReference type="eggNOG" id="ENOG502RQCB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1184869_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUL7"/>
    <dbReference type="OMA" id="NGSAPDY"/>
    <dbReference type="OrthoDB" id="4019919at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="237" mass="27381" checksum="397977FC8917EB07" modified="2011-11-16" version="1">MKLVTAIIHWLSEFPKSVVSADCNLSQFVTPFMIYNLFKFLFNEVDFNVPFEMSLDELFQLPYCELPYSFANQFNQVGSHNIISQIRILEMILANHFQNTLNNPDQIPDINLHRLILHQNATEITKYCKALVLIGTYTSKLSTVGLTTIEFLADEDKNMLEDIMAQRQRSYNKEIVPYFQSDIENRSNDAEVASSDQASNNGSAPDYIDQDDLKLLRWKVRLYEGVIYTLTMSKEPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUP8</accession>
    <name>G3AUP8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_68678</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377575.1">
        <property type="nucleotide sequence ID" value="XM_007377513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUP8"/>
    <dbReference type="STRING" id="619300.G3AUP8"/>
    <dbReference type="GeneID" id="18875233"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68678"/>
    <dbReference type="eggNOG" id="KOG1453">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_585484_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUP8"/>
    <dbReference type="OrthoDB" id="19923at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF128">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN RGD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="280"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="30"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="139"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="189"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="467" mass="53775" checksum="7704B0E9A1E7D74D" modified="2011-11-16" version="1">MNNQYSAGGGRTSLDPRRHSYIESVDNVTDNQQMSDSLQQQQQQQNHRHHHHHHHHYYKQQIFPTHYEEFHFAKPPRMNELTTPISYQTVAVDHSKTNNRSSREIHRSETTTFLNSLGNSADDDEVILRNLKKTRSRPLSTHDINTKYPFLKPPKSPVTSITKKLDQETESDNSSRDRFMPLDSVSETCMEEDEQEEDEDKTRRKWPWPHIKHMKSGLKSSDNASITTSSESIISNASSTISNLFTQAKRLSINPASNMKRTKLFTRFFKLNPDHNLFASTIQSRADYEGRQVPLIITRCIGEVEYRGLECDGIYRTAGPNSEIVAIQVAFQNNDTEKLDHLMSGDIHSVASVLKRYLNNITEPIITSDLYHDFIRVSCSPRERRISELRTLVNKLPSANKHTLKLLCNHLNLVNSKSKVNRMGFGNLATVFAPTLARNNREDMDLSIESTLFLFNKSQEIFSDCWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV10</accession>
    <name>G3AV10_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_63709</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377850.1">
        <property type="nucleotide sequence ID" value="XM_007377788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV10"/>
    <dbReference type="STRING" id="619300.G3AV10"/>
    <dbReference type="GeneID" id="18874876"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63709"/>
    <dbReference type="eggNOG" id="ENOG502S7ZI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112526_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV10"/>
    <dbReference type="OMA" id="NFEPIDY"/>
    <dbReference type="OrthoDB" id="1939598at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089713">
        <property type="term" value="C:Cbf1-Met4-Met28 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14705">
        <property type="entry name" value="bZIP_Zip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13044">
        <property type="entry name" value="ACTIVATING TRANSCRIPTION FACTOR ATF 4/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13044:SF14">
        <property type="entry name" value="CRYPTOCEPHAL, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="142"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="107"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="156"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="122"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW30084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="229" mass="26669" checksum="EFAC7CA8D34BC219" modified="2011-11-16" version="1">MNFEPTDYLQNLNLEFDSQFVSPNINQQQLDHDLDLFSSANHFFSLDVLGNDNIVPVKSEVTDPVLLPHQPQQQQTQQQQQQQPISSQQPDYSFLNFDINTQARISSDVDHEFKKPQKRSISEVNTPMSTFSDESATGAASEDKRKRNTAASARFRIKKKLKEQEMEKRSKELEEKVVMLEKKLKQLEMENKCLKNMILQQNEQKNEDLLKSIKERSILDSKSIFQYTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV56</accession>
    <name>G3AV56_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial import inner membrane translocase subunit TIM54</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_144050</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIM54 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377626.1">
        <property type="nucleotide sequence ID" value="XM_007377564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV56"/>
    <dbReference type="FunCoup" id="G3AV56">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV56"/>
    <dbReference type="GeneID" id="18870594"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144050"/>
    <dbReference type="eggNOG" id="ENOG502QPMQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039097_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV56"/>
    <dbReference type="OMA" id="RNWMIFF"/>
    <dbReference type="OrthoDB" id="5598305at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050187">
        <property type="entry name" value="Lipid_Phosphate_FormReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021056">
        <property type="entry name" value="Mt_import_IM_translocase_Tim54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358:SF101">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358">
        <property type="entry name" value="SPHINGOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11711">
        <property type="entry name" value="Tim54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW29860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="422" mass="47759" checksum="7C097E2C5F7E4D59" modified="2011-11-16" version="1">MSDTTPTDKPAAAATAATTTKTKPPKKGWSNPALRMMGIPRLSLPSRNWMIFWTVLASIGGGIAYDKYQQKQIRAKWMEKVEPLSHVTYTTEKLPRKLTIYIAPPPNDFLDESLKLFKKFIKPVLNASALDFEIFSETRQGDIRSAVAQRIRNLRQAQSEQPTEPDVKPVVPVSSGDAEEVLVSRSDLYKAKDVLGLYKIFPSEVELHPEDSDSDIAGGVVCIGRGAFKEYITGLHEGLLGPLTKPQFVIDEELKAEQELKEKAEQEDSDDEEPNLKPVPKPWIKPEQYAEASFAPELDISRVVKTSEGIPEFFEQPIYVFKVPNLIGFTTIPLKIYRYFTKRWLADDYGAKATGVVMNKTRPFTKDDLDFAADEEKEWPKKWVKTGKERNSEWVQDLQADDRIISRLKVFDVDSDLTTSNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVV5</accession>
    <name>G3AVV5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">D-serine dehydratase</fullName>
            <ecNumber evidence="11">4.3.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">D-serine deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_63627</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the conversion of D-serine to pyruvate and ammonia. May play a role in D-serine detoxification.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-serine = pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13977"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:35247"/>
            <dbReference type="EC" id="4.3.1.18"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:13978"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DSD1 family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.18" evidence="11"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377766.1">
        <property type="nucleotide sequence ID" value="XM_007377704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVV5"/>
    <dbReference type="FunCoup" id="G3AVV5">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVV5"/>
    <dbReference type="GeneID" id="18874837"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63627"/>
    <dbReference type="eggNOG" id="ENOG502QRZ0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031639_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVV5"/>
    <dbReference type="OMA" id="WPRFYGW"/>
    <dbReference type="OrthoDB" id="20198at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008721">
        <property type="term" value="F:D-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036088">
        <property type="term" value="P:D-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000016">
        <property type="entry name" value="D-serine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.20">
        <property type="entry name" value="D-serine dehydratase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051466">
        <property type="entry name" value="D-amino_acid_metab_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026956">
        <property type="entry name" value="D-ser_dehydrat-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042208">
        <property type="entry name" value="D-ser_dehydrat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28004:SF2">
        <property type="entry name" value="D-SERINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28004">
        <property type="entry name" value="ZGC:162816-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14031">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01119">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0216">Detoxification</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="D-serine dehydratase-like" evidence="14">
        <location>
            <begin position="301"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00066349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00075219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW30000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="418" mass="46037" checksum="4A655BE1EBE38C80" modified="2011-11-16" version="1">MKYPSEFTPVANKQELCKQYVGKSIKELPTPSIVIDRSQFRANCRQMIENAKKLNADFRPHIKTHKTVEGCIEQLGDGEVFTDRIVVSTMMEAWGVLQIPQVQDVHYSLPVVKSRIPEFASFSSRVSHFRLMLDQIEQLEALAAYREANPSIKKWSIFIKVDMGTHRAGLPVGDELGSLLQKALKDPRIKTHVEIYGLYCHAGHSYSSTSEKMAKDFLIDEINFANDAALLAKSLDPSLQLVLSVGATPTANASSSLTNEELLEKLHNHKLEGKLELHAGNYSCCDLQQLATGCITQATISLTVLSEIVSTYPGRGDNAPGEQLIDAGVLALAREFSAIPGHGKVIEPKGFGEWTVGRLSQEHGVLTPSDSSCKFIPYGTQVRVIPQHACIAAAAHPFFFVVGDDNTVVDVWVPLRGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE27</accession>
    <name>G3AE27_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cupin type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_146934</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372973.1">
        <property type="nucleotide sequence ID" value="XM_007372911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE27"/>
    <dbReference type="STRING" id="619300.G3AE27"/>
    <dbReference type="GeneID" id="18870734"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_146934"/>
    <dbReference type="eggNOG" id="ENOG502R6B0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060572_1_0_1"/>
    <dbReference type="InParanoid" id="G3AE27"/>
    <dbReference type="OMA" id="ANKIEPW"/>
    <dbReference type="OrthoDB" id="2205143at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02216">
        <property type="entry name" value="cupin_GDO-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047183">
        <property type="entry name" value="GDO-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41517">
        <property type="entry name" value="1,2-DIOXYGENASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41517:SF1">
        <property type="entry name" value="CUPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="3">
        <location>
            <begin position="96"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="325" mass="37041" checksum="7176E167A7B23EC9" modified="2011-11-16" version="1">MAPVESTTTASDEKVNFFKSLKPQNVEPLWTVMSAMVPPVPKPKAVPTVWNYKKLRPLLEESGRLVPPEESERRVLMLINPALKAPQTTDTLYAGLQYINPGEVAPAHRHSAFALRFIIEGDGGFTAVEGQRIFMERGDVILTPRWEWHDHGKQGDGPMIWLDGLDLPMFQAIPVNFAEHYKEERYPAETVEHSNIKFPWKITQDYLDSVEGDYAIYNYTQGDDKKDLSTIVTAQAERINAGKTSEWRQENASFVFHIYEGKGHTVIQEENGEETILTWEGKDTFCVPSWKKFRHVNEGSETAYLFSFNDSALLKNLNIYRSATN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE28</accession>
    <name>G3AE28_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BioF2-like acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_132501</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372974.1">
        <property type="nucleotide sequence ID" value="XM_007372912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE28"/>
    <dbReference type="STRING" id="619300.G3AE28"/>
    <dbReference type="GeneID" id="18869662"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132501"/>
    <dbReference type="eggNOG" id="ENOG502SI3G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017660_0_0_1"/>
    <dbReference type="InParanoid" id="G3AE28"/>
    <dbReference type="OMA" id="WWSANIS"/>
    <dbReference type="OrthoDB" id="10061051at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038740">
        <property type="entry name" value="BioF2-like_GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13480">
        <property type="entry name" value="Acetyltransf_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="BioF2-like acetyltransferase" evidence="2">
        <location>
            <begin position="657"/>
            <end position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="778" mass="89135" checksum="708708E99AC8174A" modified="2011-11-16" version="1">MFKQFLGWLHSSVAVAIYCFVFSLTQAILYSKYLNWRFADPYPIIVVWVCFLYLLVLAYERYYQAIYKNQKIKITPKKEKMVDFLIVRVGKKLLWFLLFIITVLQVVLLTQGKFDNPFNLGKDFQVPHSQKATFFTIKLVAIIFSGLLLCVWWSANISNIYLAFVSANIIQILIAIALIQINIYMINAAESSSAIKKDNFFILTACFLIGQSMIGVVISVVINHKNRLHELMGLLLFLYDTVVALLGLLLLSCTIVSGAKERYPPNSVNEIPGSIPILFGCSLAMNILCFACTRYFRKRTLTTNLLVEEYDLMRLTDYQKQGWAKLINLNKKYNAGISGEHIVSLMENYSHSSLEGMTCKILRVYRDKPHETSTKPGAINKFIKKKIEEKAKLNTAFNELDHEAVLFQNDHKSKEEEEAEPLEAYKPISKNALKKLQKKNKQKKQAMDLKSLEANTEQFYQELMSTEALVLLTVIEEIDLCERIPGKVGTFLGKYFGKNGKYPLLVVKFGLLGFHWPFKRSTFYCSATKKPVARAAAVLYTLSEWNKKNEKCAVLMDPMYSDYHFEEGVTYSGWYKIKLPNSHIIDLRPFRNQKPTDYFKAIKYRTQENTFKSANGEVWETDQFNYENCSSIINMNLNIANNRASSGQSSQLMYPDWEFISNLGNNSNENKYRTLLYLKVDGKIIASCVIFRLGETMTSDIQGLDHETSKKYKAYFVMMQEVIRMGLEEKVSFIDFGPTTEEAKVSIGCKVVPLTGCIYPRNPCMGPIIKFVASKVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE44</accession>
    <name>G3AE44_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">methionyl-tRNA formyltransferase</fullName>
            <ecNumber evidence="1">2.1.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_58802</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.2.9" evidence="1"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372990.1">
        <property type="nucleotide sequence ID" value="XM_007372928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE44"/>
    <dbReference type="FunCoup" id="G3AE44">
        <property type="interactions" value="278"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AE44"/>
    <dbReference type="GeneID" id="18872612"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58802"/>
    <dbReference type="eggNOG" id="KOG3082">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033347_0_1_1"/>
    <dbReference type="InParanoid" id="G3AE44"/>
    <dbReference type="OMA" id="LPTHEID"/>
    <dbReference type="OrthoDB" id="10268103at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004479">
        <property type="term" value="F:methionyl-tRNA formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd08646">
        <property type="entry name" value="FMT_core_Met-tRNA-FMT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041711">
        <property type="entry name" value="Met-tRNA-FMT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11138">
        <property type="entry name" value="METHIONYL-TRNA FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11138:SF5">
        <property type="entry name" value="METHIONYL-TRNA FORMYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Formyl transferase N-terminal" evidence="2">
        <location>
            <begin position="27"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="229" mass="25189" checksum="3ED963D4AFE5749A" modified="2011-11-16" version="1" fragment="single">MRVRIKLNWNLSNTRSFSSVSSSDRLKIAFFGSDHFSTASLTALHTYSQLHNHTIHVITPTLKPTGRGLTQLIDLPLGTLANSLSLPVHRVENSSDMNELVSHQFNLAIAVSFGKLIPSKFLQSCKFGGLNVHPSLLPTYSGASPLQYALLNDDKFTGCTVQTLHPTKFDKGEILLQSSKVDILDSDNFESLEKKLSDIGARLLVETIDQRLFLDGGGIKSEYPFSLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEA8</accession>
    <name>G3AEA8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Suppressor of lethality of KEX2 GAS1 double null mutant protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_58850</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373054.1">
        <property type="nucleotide sequence ID" value="XM_007372992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEA8"/>
    <dbReference type="GeneID" id="18872644"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58850"/>
    <dbReference type="eggNOG" id="ENOG502RZI6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075100_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEA8"/>
    <dbReference type="OMA" id="THAINDH"/>
    <dbReference type="OrthoDB" id="4082885at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="25750" checksum="86B2589E2166BE58" modified="2011-11-16" version="1">MANSQSLAVGLAVGIPSFLIIVVVVVFWYRNNKKQTKEDLEVDAIDLELREDQSFRQFHEELHQPYVKEKASSTLIYEKAVVESGSSASEVSPNQTKPTAYDFYETFIPMLPPNQPYTNNNTHKTHTPRASSTDLINVPTSPDVSVSETSSTIDILAKQLHQPEFFEKLPSKAAKIQLKPRNPMVQSNNSSTDMINNYLVSETRGINDHFTYEATAVDIANERREQGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEB0</accession>
    <name>G3AEB0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_48618</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFB1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373056.1">
        <property type="nucleotide sequence ID" value="XM_007372994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEB0"/>
    <dbReference type="FunCoup" id="G3AEB0">
        <property type="interactions" value="957"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEB0"/>
    <dbReference type="GeneID" id="18871245"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48618"/>
    <dbReference type="eggNOG" id="KOG2074">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019188_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEB0"/>
    <dbReference type="OMA" id="NRPNFDM"/>
    <dbReference type="OrthoDB" id="360521at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13229">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027079">
        <property type="entry name" value="Tfb1/GTF2H1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013876">
        <property type="entry name" value="TFIIH_BTF_p62_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12856">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08567">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BSD" evidence="8">
        <location>
            <begin position="227"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="103"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="620"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="105"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="620"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="642" mass="71376" checksum="C29F8DE01CB2B743" modified="2011-11-16" version="1">MDIVRGACSVDKVGGMVYIREDLAPSILEWKAIEADKTLSIPLNSLTNLQSTKETSPKMILKIVYKDDNGEEVPVKFTFSNRPTMNNVKDSLQAIVARSRTRVEATPVSATATPPASSAGGTPSTTTPTGSASTSAAASTSTAAVPAATVVDLSDAALLKNLALQQKLLYEDKTLRDTFTKTVITLNLSPTIFWSSRIPQLRTFALTISQHRGPYNVLSTIKPVATSDNQVNVNVTREMINEIFEIYPVIKLAFDDLVPHKFNEGEFWSRFFNSKLFRRLRGDKINLGNIRGDVVLDKYLYADDTRKRKGSVMEVDNDAEEENRKRQDVSVHKFLDLLGNEEDNSQKLGNKPDFTMRYDDTTASKTTGENEMVVLMRNMNKLSSKMMSVAEPEEQEKEKPEFKGLSSVEFHEYDEELNLHDLNDITSQEYVKLNIDPNLTTHSAQHTSQADVTALQLSEYLKNQFTSPRVDLTDTYNGKTADIERSSNEITTLTKHNFRNYKSIDNRGTNIVPKEISQEIIGYNITTTEFLSHFWGLFLRGNNPGQLRKLFTSLKNSKTGITELKTKAMAKLEQIEVVKASEKVKERVARELNACLEVLEVAIDRACSEYIAAVKQSTGASATNGTPAIGTSTPIAGTPVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEF9</accession>
    <name>G3AEF9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 20">DNA topoisomerase 2</fullName>
            <ecNumber evidence="7 20">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">SPAPADRAFT_131140</name>
    </gene>
    <organism evidence="25">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18 20">Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19 20">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6 20">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 20">Belongs to the type II topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="7 20"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373159.1">
        <property type="nucleotide sequence ID" value="XM_007373097.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AEF9">
        <property type="interactions" value="1212"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEF9"/>
    <dbReference type="GeneID" id="18869555"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131140"/>
    <dbReference type="eggNOG" id="KOG0355">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001935_1_1_1"/>
    <dbReference type="InParanoid" id="G3AEF9"/>
    <dbReference type="OMA" id="DVKPHMI"/>
    <dbReference type="OrthoDB" id="276498at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000819">
        <property type="term" value="P:sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16930">
        <property type="entry name" value="HATPase_TopII-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03481">
        <property type="entry name" value="TopoIIA_Trans_ScTopoIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03365">
        <property type="entry name" value="TOPRIM_TopoIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.40:FF:000011">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.30:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000008">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000004">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.670:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.199.10:FF:000002">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="entry name" value="Topoisomerase II, domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="entry name" value="Topoisomerase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050634">
        <property type="entry name" value="DNA_Topoisomerase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001241">
        <property type="entry name" value="Topo_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013506">
        <property type="entry name" value="Topo_IIA_bsu_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001154">
        <property type="entry name" value="TopoII_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031660">
        <property type="entry name" value="TOPRIM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034157">
        <property type="entry name" value="TOPRIM_TopoII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169:SF38">
        <property type="entry name" value="DNA TOPOISOMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169">
        <property type="entry name" value="DNA TOPOISOMERASE/GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00204">
        <property type="entry name" value="DNA_gyraseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16898">
        <property type="entry name" value="TOPRIM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01158">
        <property type="entry name" value="TOPISMRASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00418">
        <property type="entry name" value="TPI2FAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00433">
        <property type="entry name" value="TOP2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="Type II DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52040">
        <property type="entry name" value="TOPO_IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15 19 20" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="16 19 20" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14 19 20" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="22">
        <location>
            <begin position="500"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IIA-type catalytic" evidence="23">
        <location>
            <begin position="751"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1241"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="1260"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="1303"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="1335"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="1361"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="1406"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="19">
        <location>
            <position position="841"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00019635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00053943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS52040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="EGW35747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1429" mass="161902" checksum="9CA224953C6D3207" modified="2011-11-16" version="1">MSSESDASDYYSDGSDDFVPSPPTKKKIASKTSKSPLSDSTNNTSGSSTPSTQVGTGPKSNASETYQKLSQLEHILKRPDTYIGSVEKTKMEMWCFDAESESMKFKEVTIVPGLYKIFDEILVNAADNKIRDPTMKNIRVKIDPENNVIQVMNDGKGIPVEIHTKENMYIPELIFGNLLTSSNYNDDEKKVTGGRNGYGAKLCNIFSTEFELETADLNTGKLYKQTWTNNMGKVSKPKITTLKTKKEYTKISFKPDLAKFDMDSLDEDILAVLRRRVYDLCGTVKNCNIYLNDKRLSISNFKSYIEMYVNAIKERSPEPEGEESKNYTTIVHEVFNDRWEIAFAVSDGSFNQVSFVNSIATTSGGTHVKYVSDQIITKLVEVLSKREKGKKKLMIRPQEVRDNMFIFLNCLIENPAFTSQTKEQLTTKPSQFGGKDKFVPSDNLINRILKTGIADKIRSIARANEDKALQKADGSRKNRIKGQVNLVDANKAGTKDGLKCTLILTEGLSAMNLAVAGLSVVGRDYYGCFPLRGKLLNVREASADQIAKNAEINALKQIIGLQHKKVYTQESIKSLRYGHIMIMTDQDQDGSHIKGLIINFLETSFPGLLDIPGFLLEFITPIVKVTVKGRTKRVISFYNMPEFERWRDTEGTQCRWTQKYYKGLGTSTPIEAREYFSALDKHLKRFHALQGDDKECIDLAFSKKKADDRKEWLQGFLPGTHLDPDLNDIPISDFINKELILFSMSDNVRSIPSVLDGLKPGQRKVLFGCYKRKLRSEIKVAQLAGYVSENTGYHHGEQSLIQTIIGLAQNFIGSNNINILKPNGSFGSRAAGGKDYSAARYIFTEINEITRTIFNPLDDPLYTYVQDDEQTVEPTWYLPVLPMILVNGAEGIGTGWSTNIPSYNPKDIVANIRRLMNGEPLQEMTPWYKGWEGDIEPMGPQKYKVSGRIEQVDNNTVEITEIPVKTWTNNVKEFLLAGFGNEKTQAWIKDMEEHHTTSIRFVVKLSDAEMEKSLKVGLLERFKLVSSLSLGNMVAFDPMGRIKKYNDVLEILKDYYYVRLEFYQKRKDYMSEDLQNQLMMLSEQARFIKMIIEKKLSVANKKKKELVSLLQQHKFIKFSRQGKPIKEELFLNDDDSIEEEEEIVEASDGDISALNLKQVETDKNSTEHVPETVYSSYEYLLGMTIWSLTYERYLKLLKQRDIKEAELNELLSKSAKDLWNEDLERFMVEFDKFLIRDQEERESLAASGDKNKKKRTRKAPAKEPPKKKIKSEPIVKSEPVVTPASPKPIEKKSDVLSFFSPTSSSSSSKSKAGSSKKKPVTLFSDSEDDELIFGSSNNSTPKKSIKKSPSSEPKKTKKKTSIKDELDDLKIEGKLMDTISSSGSRRPARERKTTAKRALTPAYVIDSDEDEDDDIEVLDDEDDEEYGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF06</accession>
    <name>G3AF06_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Alpha-aminoadipate reductase</fullName>
            <ecNumber evidence="6">1.2.1.31</ecNumber>
            <ecNumber evidence="5">1.2.1.95</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">L-aminoadipate-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LYS2</name>
        <name evidence="19" type="ORF">SPAPADRAFT_145301</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(S)-2-amino-6-oxohexanoate + AMP + diphosphate + NADP(+) = L-2-aminoadipate + ATP + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46936"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="1.2.1.95"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(S)-2-amino-6-oxohexanoate + NAD(+) + H2O = L-2-aminoadipate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12308"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
            <dbReference type="EC" id="1.2.1.31"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(S)-2-amino-6-oxohexanoate + NADP(+) + H2O = L-2-aminoadipate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12304"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
            <dbReference type="EC" id="1.2.1.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pantetheine 4'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:47942"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.31" evidence="6"/>
    <dbReference type="EC" id="1.2.1.95" evidence="5"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372222.1">
        <property type="nucleotide sequence ID" value="XM_007372160.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AF06">
        <property type="interactions" value="730"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF06"/>
    <dbReference type="GeneID" id="18870682"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_145301"/>
    <dbReference type="eggNOG" id="KOG1178">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_19_0_1"/>
    <dbReference type="InParanoid" id="G3AF06"/>
    <dbReference type="OMA" id="ENDKFTM"/>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00032"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004043">
        <property type="term" value="F:L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd17647">
        <property type="entry name" value="A_NRPS_alphaAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05235">
        <property type="entry name" value="SDR_e1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000046">
        <property type="entry name" value="L-2-aminoadipate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000787">
        <property type="entry name" value="L-2-aminoadipate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000035">
        <property type="entry name" value="L-2-aminoadipate reductase large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.10:FF:000020">
        <property type="entry name" value="Peptide synthetase mbtE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.30">
        <property type="entry name" value="Nonribosomal peptide synthetase, condensation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010071">
        <property type="entry name" value="AA_adenyl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014397">
        <property type="entry name" value="Lys2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010080">
        <property type="entry name" value="Thioester_reductase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01733">
        <property type="entry name" value="AA-adenyl-dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03443">
        <property type="entry name" value="alpha_am_amid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01746">
        <property type="entry name" value="Thioester-redct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44845">
        <property type="entry name" value="CARRIER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44845:SF1">
        <property type="entry name" value="L-2-AMINOADIPATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001617">
        <property type="entry name" value="Alpha-AR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="11 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="18">
        <location>
            <begin position="849"/>
            <end position="926"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW34810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1400" mass="154720" checksum="1E35F69C4A766E43" modified="2011-11-16" version="1">MSDYWLNYLVNPTLSVLPHDYLKPANNKSVEGTYTFDIPGKSSDDFIFGLSVFAALVYRLTGDEDIVLATDAPNDPTEFIIRLNLTPEITFGDLVAKITSEYETSRSHVNYKTLSEVSQTIKDANKLETNPALFRLSYQHANENQQLNTTVEGSIRDLAVYTDGKKFTIFYNALLYSHDRIVILGEQFNQYISSVSENQDIAITKVNLVTESQRAQLPDPTLDLDWSGYRGAIQDIFMANAEAHPDRTCVVETESFMDPSSKTRTFTYQQINQASNIVGNYLKETGIKKGDVAMIYAYRGVDLMIAVMGVLKAGATFSVIDPAYPPSRQNIYLSVARPRALIGLEKAGQLDQLVVDYIKDELDVISTIPQLRVNDDGSLVGGSIEGSSGDCLADYQKFKDQRTGVIVGPDSNPTLSFTSGSEGIPKGVLGRHYSLAYYFPWMAKRFNLSENDKFTMLSGIAHDPIQRDMFTPLFLGAQLLVPTADDIGTPGKLAEWMAKYGATVTHLTPAMGQLLSAQASTPIPSLHHAFFVGDILTKRDCLRLQSLAENVYIVNMYGTTETQRAVSYFEIKSRASDSLYLKNLKDVMPAGTGMHNVQLLVVNRNDRSQTCGVGEIGEIYVRAAGLAEGYRGLPDMNAAKFITNWYVDPSQWVKQDEANKNPEQTWREHGWFGPRDRMYRSGDLGRYLPDGNVECCGRADDQVKIRGFRIELGEIDTHLSQHPLVRENVTLVRRDKNEEPTLISYIVPKESPELHNFKSEIDDSAEEANDPIVRGLVAYRELIKDIKAYLKKKLASYAIPTIIVPLVKLPLNPNGKVDKPKLPFPDTVQLAAVSKLSISKQQGEEIEQEKLTALEQQIKDLWFEVLPNRPATISKQDSFFDLGGHSILGTRMIFELRKKLNVEIPLGVIFKNPTVEQFAREVETIIKGNDVVELADEGETEREAKKEEPVINYAKDAVELSKSALLSSYPSLKGLDSSKTINVFVTGATGFLGSFIVRDLLTSRKGLDIRVFAHVRASSVEAGLDRLRQTGTTYGIWDEEWTSKIEVVLGDLSKKQFGLADDAWTKLTNTVDVIIHNGAFVHWVYPYSQLRDANVIGTINVLNMAGEGKAKYFSFVSSTSALDTDYFVNLSDELLAQGKQGISEADQLAGSAKGLGNGYGQSKWAAEFIIRRAGERGLKGCITRPGYVTGFSETGASNTDDFLLRMLKGSAELGLYPDITNKVNMVPVDHVARVVTATALHPPVESELAVAHVTGHPRIHFNDFLGCLKTYGYEVNSADYPVWKTALEKYVVEQANESALFPLLHFVLDNLPQDTKAPELDDTNAATSLKLDAEWSGVDASEGKGVTPDQTGVYISYLIKIGFLPGPTGTGEKKLPEVELSKETLSLISQGAGGRGSAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF31</accession>
    <name>G3AF31_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dtdp-glucose 4,6-dehydratase</fullName>
            <ecNumber evidence="2">4.2.1.46</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">GAL102</name>
        <name evidence="2" type="ORF">SPAPADRAFT_132097</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.1.46" evidence="2"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372247.1">
        <property type="nucleotide sequence ID" value="XM_007372185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF31"/>
    <dbReference type="STRING" id="619300.G3AF31"/>
    <dbReference type="GeneID" id="18869636"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132097"/>
    <dbReference type="eggNOG" id="KOG0747">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_14_1"/>
    <dbReference type="InParanoid" id="G3AF31"/>
    <dbReference type="OMA" id="KLIPLMC"/>
    <dbReference type="OrthoDB" id="331544at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008460">
        <property type="term" value="F:dTDP-glucose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000304">
        <property type="entry name" value="UDP-glucose 4,6-dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="1">
        <location>
            <begin position="10"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="328" mass="37933" checksum="3C0549C8E25DAC8A" modified="2011-11-16" version="1">MTIHFDKRVLITGGAGFIGSNFLSYFVRKYPNYHFTCIDKLNYASNFENIADLLQLNNFKFIKLDLSESFETLYSMLVTNFHQMEITDFINFAAESCVDRSLISPLYFTKNNILATQNVLECYRELRNKYSHQYKFNFIHISTDEVYGDINQNTDEQGTLCPTNPYAATKAAIDLIIQSYQYSYNLPITILRPNNVYGPNQYPEKIIPLTIKCLQEQSPIPIHGDGTNKRCYLYISDFLHAIDLIWHSGISTSEIYNIGGDCEIDNNSLVKLICQKVFKTNSVDHTQYIQYVKDRGYNDKEYSINNDKIRQLGWKPVVVLPEGLDMII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFC6</accession>
    <name>G3AFC6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">54S ribosomal protein L27, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_58037</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL41 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372328.1">
        <property type="nucleotide sequence ID" value="XM_007372266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFC6"/>
    <dbReference type="FunCoup" id="G3AFC6">
        <property type="interactions" value="140"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFC6"/>
    <dbReference type="GeneID" id="18872295"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58037"/>
    <dbReference type="eggNOG" id="KOG4756">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1778531_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFC6"/>
    <dbReference type="OMA" id="KHTKYGE"/>
    <dbReference type="OrthoDB" id="408933at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019189">
        <property type="entry name" value="Ribosomal_mL41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21338:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21338">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN L41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09809">
        <property type="entry name" value="MRP-L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="168" mass="19316" checksum="197756D4160D2660" modified="2011-11-16" version="1">MRATQVLNFQSTASSSLRRPWQKYKNGQLWYGFIKSGSKRHPITTKQGNHTFYKGTGSTGVGRLNNKGQYIMNWDKVRTYVVPSDLNSTELKALVSPNTPQIRLTTEGYKDGIKSAEYAFDSIIKFVEYGEEYDQQDLEKQDYEERIVSPEILEKESAEKLELNTSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFQ3</accession>
    <name>G3AFQ3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="19">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">SPAPADRAFT_48092</name>
    </gene>
    <organism evidence="25">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 19">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Membrane</location>
            <topology evidence="19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="EGW35042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372454.1">
        <property type="nucleotide sequence ID" value="XM_007372392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFQ3"/>
    <dbReference type="FunCoup" id="G3AFQ3">
        <property type="interactions" value="364"/>
    </dbReference>
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    <dbReference type="GeneID" id="18871175"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48092"/>
    <dbReference type="eggNOG" id="KOG0210">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000846_6_0_1"/>
    <dbReference type="InParanoid" id="G3AFQ3"/>
    <dbReference type="OMA" id="IAITTWH"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
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        <property type="project" value="EnsemblFungi"/>
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    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180013">
        <property type="term" value="F:lysophosphatidylserine flippase activity"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090555">
        <property type="term" value="F:phosphatidylethanolamine flippase activity"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140346">
        <property type="term" value="F:phosphatidylserine flippase activity"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000085">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000009">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF5">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 18 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 19" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="439"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="463"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="971"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1000"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1044"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1078"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1107"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1138"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="22">
        <location>
            <begin position="163"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="21">
        <location>
            <begin position="272"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="23">
        <location>
            <begin position="937"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="16">
        <location>
            <position position="526"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="526"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="526"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="527"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="528"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="528"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="625"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="668"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="692"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="721"/>
        </location>
        <ligand>
            <name>ATP</name>
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    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="802"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="803"/>
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        <ligand>
            <name>ATP</name>
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    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="804"/>
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        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="884"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="890"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="910"/>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
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    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="913"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="914"/>
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        <ligand>
            <name>ATP</name>
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    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="914"/>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00049128"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
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    <evidence type="ECO:0000256" key="18">
        <source>
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    <evidence type="ECO:0000256" key="19">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00122"/>
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    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="EGW35042.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1176" mass="132601" checksum="FAD1CD670D20FDA1" modified="2011-11-16" version="1">MSSSPKRYSFGDDTDSFDDLDNSLDRALHSASNNLFTYSQNTPSRNASFGSAGAIPLTTIRSDSHEHERIEDNDTSSDMSPLIANSAYSHNHNLSLSSSSSSLNMAARSMSSRFKRAFRRSINLFNMAKRELDQERRRKSSTVQGMENDHPDTERCISAKTTNARSNKTYPSNAISNAKYNPITFIPIILYEQFKFFFNLYFLIVALSQIIPQLRIGYLSSYIVPLAFVLTVTMMKEAGDDIARRRRDKEQNNELYEVLNRGTSDAQLTVETKLVPAKNLRVGDLVRLHKDVRIPADMILIQSSDHTGESFIKTDQLDGETDWKLRIAPNVTQKMTNVTEMINTVSLIVGSPSKSIHHFNGKLVHHPQSSAGSASPNSHNQSFPITVDQTLWANTVLASGTAIGMVVYTGIETRQSMNTTMSGVKIGLLELEINSLSKILCVVVFILSVVLVLANGFPLKKGWYIDIMRFLILFSSIIPVSLRVNLDLAKSVYASQIQKDDDIPSTIVRTSTIPEDLGRIEYLLSDKTGTLTQNDMELKKLHLGTVSYAGETLDIVTDYVSSLLAYLDANPAGSISKKKKDLSSRVCDLVITLALCHNVTPTYEEDTDEYSNFTEVTYQAASPDEIAIVKFCESVGLRLFKRDRHSITLFHTKTNQTLEYEILYNFPFSSETKRMGIIVKDKQRDETWFMQKGADTVMATIVSTTDWLDEETGNMAREGLRTLVIGRKKLPDHLYNEFVESYQKASLAMHDRDSAMQKVISYYLEQDLELLGLTGVEDKLQLNVKTSIELLRNAGIKIWMLTGDKVETAKCVSISARLISRGQYVHEITKLGHPDMALSQLEYLRINTNACLLIDGESLGTYMKYYRDEFFKVVIKLPAVIACRCSPQQKADVAVMIRTFTGKRVCCIGDGGNDVSMIQCADVGVGIVGKEGKQASLAADFSIDQFYYLSKLLLWHGRNSYKRSAKLGQFIIHRGLLISVAQAVYSISSKFEPLALYQGWLMVGYSTFYTMAPVFSLTLDCDIDEHLTKLYPELYRELTLGKSLSYKTFFMWVFISLFQGSVIQLFSQQFHSLADSKFTIMVALSFSCLIYNELIMVAISIYKWNRVMAATIIITLLIYIGSIPFLTDYFDLKYVWSISYLWQVSLILVVSLFPVWLVQYLNRKLRPPSYAKVQQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFQ5</accession>
    <name>G3AFQ5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_58177</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372456.1">
        <property type="nucleotide sequence ID" value="XM_007372394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFQ5"/>
    <dbReference type="FunCoup" id="G3AFQ5">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFQ5"/>
    <dbReference type="GeneID" id="18872359"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58177"/>
    <dbReference type="eggNOG" id="KOG2386">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013931_1_0_1"/>
    <dbReference type="InParanoid" id="G3AFQ5"/>
    <dbReference type="OMA" id="PGIRHGH"/>
    <dbReference type="OrthoDB" id="428974at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004484">
        <property type="term" value="F:mRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051029">
        <property type="entry name" value="mRNA_Capping_Enz/RNA_Phosphat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367:SF25">
        <property type="entry name" value="DUAL SPECIFICITY PHOSPHATASE CATALYTIC DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G03540)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="506"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="5">
        <location>
            <begin position="572"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="649" mass="72780" checksum="7E167AB248A2E8FF" modified="2011-11-16" version="1">MSSDEESRELLAVSASTSPEPVSRPHLAKLSQLSQAQVEQTNTTLKRFCEFFKINFLGYQNQFEEYVLQNEPNDCSLVAKYCKVIDIDLRNQNTISVDNVTVNSLHIQHPLNNFMSNEANQLSPENVDISKELEEAPILVFVHGLGGQMSQFEPLMGLLSQCSEIVSLDLPGFGNSKIQFSDKLKTVSNISDEDKQRISSSIQKMTWSDFTTENITNILYEFIKQQIPENKKIVLIGHSMGTHLSIKLAKKLPNHKVEGLILLSPPGLRDDVNVGVKDSGHKLFSLFKLFTYVPWLFNLFRSWDRLGGLNSTSVARQLSSGSNTIYNKLKQLRWNMDIDSSIILKYSNGFSPATTSDLTTAVSQFNDNPTDKHVYEKTLVLGGSSDSVTPVKNIYKIDEFLTRTFDRKVSSTIEIKGVGHSLLLSKPEFISGMILNHVESKFPQRLHLSPAWVLQVKARISGDKWGLKNELKWSLLKPISSNITRRNGSDIAPLLGMKTLREGDANHSPTILEGLFYGENSHNSADIPKGNLIAIIDISADIPPYSPKSFNVIKYYKCATVSKVAPDQSAIRRFIQLVDDILSDNKAEHPLIAVHCHYGFNRTGFLVCCYLIEVLGWSVQEAVEGFKNAKPPGIKHPHFIDALYVRYES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFS4</accession>
    <name>G3AFS4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">MRH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_133026</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372475.1">
        <property type="nucleotide sequence ID" value="XM_007372413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFS4"/>
    <dbReference type="FunCoup" id="G3AFS4">
        <property type="interactions" value="227"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFS4"/>
    <dbReference type="GeneID" id="18869709"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133026"/>
    <dbReference type="eggNOG" id="KOG4504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053195_1_0_1"/>
    <dbReference type="InParanoid" id="G3AFS4"/>
    <dbReference type="OMA" id="YTIGICS"/>
    <dbReference type="OrthoDB" id="4504960at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028927">
        <property type="entry name" value="Man-6-P_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071:SF0">
        <property type="entry name" value="MANNOSE 6-PHOSPHATE RECEPTOR-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02157">
        <property type="entry name" value="Man-6-P_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="264"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="11">
        <location>
            <begin position="93"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW35063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="301" mass="33980" checksum="15F9CC36BC0D5556" modified="2011-11-16" version="1">MNHKSWLKILRRVTLPLLTFTLIVCITIIQTNKPKDTLYKTGTQIFDSISHFTFTKDELLPQAQPDEALFSPSEAEQSGSDDKSETEEEEKLDPCTIINPNTKQFIDLRGLSSFANDGKALPWNTRGYDSGRNYTIGICSNPFKKFHDSWNEIQDGLDPSKIGAYYIDPATNKYVSMGEYATTPVFRGRKLTLTYENGAFCDAVDANTGKKLKKSTILTFTCDREMMNSKASISFVGQMNNCSYFFEVRSHHACPTAAKTNETGAIWIFLLIVLAALLVYFSGGLLYKQMKRKPKREKSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFZ9</accession>
    <name>G3AFZ9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58327</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372550.1">
        <property type="nucleotide sequence ID" value="XM_007372488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFZ9"/>
    <dbReference type="GeneID" id="18872403"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58327"/>
    <dbReference type="HOGENOM" id="CLU_3093233_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFZ9"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="52" mass="6184" checksum="83C00770F673CBE6" modified="2011-11-16" version="1" fragment="single">MSKYTRVYNKRIKNILPLLVILVLIVWTGRQYLTEAVVQMKLENAALLHHKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG14</accession>
    <name>G3AG14_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RAM signaling network component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_48189</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372565.1">
        <property type="nucleotide sequence ID" value="XM_007372503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG14"/>
    <dbReference type="STRING" id="619300.G3AG14"/>
    <dbReference type="GeneID" id="18871190"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48189"/>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011502_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG14"/>
    <dbReference type="OMA" id="NVHRACQ"/>
    <dbReference type="OrthoDB" id="1394818at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019487">
        <property type="entry name" value="RAM_signalling_pathway_SOG2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF9">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10428">
        <property type="entry name" value="SOG2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="212"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="319"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="417"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="344"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="369"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="422"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="448"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="470"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="928" mass="101676" checksum="904407ACD570DD93" modified="2011-11-16" version="1">MQMNKSAILATLRDQLESIDQNQTIRLNGLNANTNTTLNFNDVVDCIFEFIHSFDPPLVVEKLSLENNHFSELPSNFSKISPSLKYLDLHHNSISNIPNDYFHKYFKQLEVLDLSSNKLSMLPESVSELINLKVLSIKDNKFKYLSPTLGNLPHLNLIEVANNPLILPSNDMIRAFQKQKSDLDWVSELKSFLLANRAVLYQKIDEGILKESRRSYEEQSQQQQQQQQQQQQQLLGEQQPLPPYVTPPSIRRSQSVSEGTKAKSSKASRRMGLIIKKSDESNGSKIVNNTLDDNSNVHTTLQFNPADFLTSGIPHSASAIETHFPSSPPQLKVQTSMEASSMGATSAAQSLNSALNSPSHTTPKSALTPPSSVRPSRSRSNTLKEIDRILENSASVDTEHKSSAYFRRLSTLQEQPLDEFETSTTAASTATKEVSQPKIIPIPEKPSTESIKPSISLSHVPKNGLPVASSEGSSPVRTPSQKKQYSSTVIKVSRKVLFSFSELHSSIRRFTGFCSDKKITMKMVSFLYSTKANIDSLVENLEIMEEMGNNLDQIVTSLQTCISSFKSMMNLLHESFSTFVNKIDVCFIRMLYLSIYGSFNELHNAYMLLVPKQQQQQQQQQLPPQTQAKLPSVSQLVTTTEATKPKIVSTPSAMSVENSEEMDEKLYITIETATATAQTSLVELNQALLKNVPGDIQPNVASRVKELTNLCTSSSEITKRLSTRLITIRNNPSQTTKKLFAEDINQFIKTMVQTLAAVKAIVKDLPILDDIRASMSNLTKTAKEVTYMLEVSAYKSLLPESSGGGGGAAAAAAAASSGATGTTNPPPTIQQVQSLVNSVPNVFTPGSGHPPTLASTISSNSIFSGGSGPTPVRTPMGPMSNQSDGSNSVISPIGSMATTPSPLATVQPTKTGNPFDNMFMRADTSNNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG69</accession>
    <name>G3AG69_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTH La-type RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_58408</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372620.1">
        <property type="nucleotide sequence ID" value="XM_007372558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG69"/>
    <dbReference type="FunCoup" id="G3AG69">
        <property type="interactions" value="731"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG69"/>
    <dbReference type="GeneID" id="18872440"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58408"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043291_0_1_1"/>
    <dbReference type="InParanoid" id="G3AG69"/>
    <dbReference type="OMA" id="WRIYEAN"/>
    <dbReference type="OrthoDB" id="439993at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002344">
        <property type="entry name" value="Lupus_La"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF140">
        <property type="entry name" value="ACHILLES, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00302">
        <property type="entry name" value="LUPUSLA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="6">
        <location>
            <begin position="5"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="218"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="238"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="287" mass="33379" checksum="C0ADF4B474080F1D" modified="2011-11-16" version="1">MSDFVYQGEDFDEKVRKQVEFYFSDSNLQTDKFLWKIYQANEGWVELKTILTFGRMRQYRPEEKVIAALKESSQLVLSESGDMIKRKEPVKDLDEIKNTRKKNTIHIEGFPKDATQEDLESWFQEKIVVSLPKEQGISSLRRIKSRAKKEFFGVVDVELKTLEDAEYLLNEVELAYPQGIVTATEEEDKKNLLKKMSLLRFQEMRESAKRFGVNEVTKRRNSFNDNQKGKKFGKNFKGKREPRETTEETKLEKVVESEPAAEAEKVAEKTSEEAIAKEESKPAEPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG96</accession>
    <name>G3AG96_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein 135 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58439</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372647.1">
        <property type="nucleotide sequence ID" value="XM_007372585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG96"/>
    <dbReference type="GeneID" id="18872451"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58439"/>
    <dbReference type="eggNOG" id="KOG1398">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012946_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG96"/>
    <dbReference type="OMA" id="SAAIVMW"/>
    <dbReference type="OrthoDB" id="4021778at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026749">
        <property type="entry name" value="Tmem135"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12459:SF15">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12459">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 135-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="43548" checksum="6D1888BE8E46DFE2" modified="2011-11-16" version="1">MIKALHPRKFPMFAARLIAGINLIEPLMFTILKKTKLFSHRSTTNVFVSTLFSALLSALVNFPTYQNHIIGYDRFNSLDLTLLVATRAIDTVFSSELGKIVPTWLVPYGDVVLFTVSSAFIMYSWFFHVERLPPAYHQWITSAANMDHDIYEVLRLIKSKKLVYGEPGPYSDFLGPYFEKYGKDPKDANTTINLPISCEAVHAFKTKSCELHALWRFYRGFKFAFTVYGPLNLLLLLLPKKKKMSQRLLRALTTSVRSSAFLGAFIGIYWYSVCLARTRLFPLLAPKVPRTRYDDTIAPAMGAIMCGLSSLVETPQRRKELALFVAPRGLGTLVSADATTENLQIESIVFSVCLAILVAYSKSKPSTVRGIFGKGLNLVFNIDYYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGZ4</accession>
    <name>G3AGZ4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial</fullName>
            <ecNumber evidence="6">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="6">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_57716</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer of an alpha and a beta subunit. Different beta subunits determine nucleotide specificity. Together with the ATP-specific beta subunit SUCLA2, forms an ADP-forming succinyl-CoA synthetase (A-SCS). Together with the GTP-specific beta subunit SUCLG2 forms a GDP-forming succinyl-CoA synthetase (G-SCS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 8">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="6"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372079.1">
        <property type="nucleotide sequence ID" value="XM_007372017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGZ4"/>
    <dbReference type="FunCoup" id="G3AGZ4">
        <property type="interactions" value="822"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGZ4"/>
    <dbReference type="GeneID" id="18872166"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57716"/>
    <dbReference type="eggNOG" id="KOG1255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052104_1_0_1"/>
    <dbReference type="InParanoid" id="G3AGZ4"/>
    <dbReference type="OMA" id="VIICITE"/>
    <dbReference type="OrthoDB" id="1664372at2759"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009361">
        <property type="term" value="C:succinate-CoA ligase complex (ADP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004776">
        <property type="term" value="F:succinate-CoA ligase (GDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006104">
        <property type="term" value="P:succinyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000005">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004230">
        <property type="entry name" value="PRK05678.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117:SF2">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP_GDP-FORMING] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117">
        <property type="entry name" value="SUCCINYL-COA LIGASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01798">
        <property type="entry name" value="SCOASYNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA-binding" evidence="9">
        <location>
            <begin position="26"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="6 7">
        <location>
            <position position="275"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="39"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="118"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="322" mass="33399" checksum="8E4BC59138873409" modified="2011-11-16" version="1">MYKQFVRGFASSAPARNYASTIKNLKITKDTKVIYQGFTGKQATFHAEQAIAYGTQVVGGINPRKAGSTHLDLPVFGTVADAMKEAGATATGIFVPPPLAAAAIEEAIAAEIPLAVAITEGIPQKDMVRIAQILKTQDKTRLVGPNCPGIIAPDQCKIGIMPSSIHKKGKVGVISRSGTLTYEAVAQTTAVGLGQSLVIGMGGDPFPGTNFIDALTLYLSDPETEGIILIGEIGGSAEEEASEFLKQHNLTRDVPKPVVSFIAGVSAPPGRRMGHAGAIVAGGKGDAKSKIAALESAGVVVEKSPARLGKSLLAEFENKGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH50</accession>
    <name>G3AH50_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Shr3 amino acid permease chaperone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_58715</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372893.1">
        <property type="nucleotide sequence ID" value="XM_007372831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH50"/>
    <dbReference type="FunCoup" id="G3AH50">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AH50"/>
    <dbReference type="GeneID" id="18872572"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58715"/>
    <dbReference type="eggNOG" id="ENOG502RXJB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080510_1_0_1"/>
    <dbReference type="InParanoid" id="G3AH50"/>
    <dbReference type="OMA" id="ANMLFDG"/>
    <dbReference type="OrthoDB" id="5229808at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013248">
        <property type="entry name" value="Psh3/Shr3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28228">
        <property type="entry name" value="SECRETORY COMPONENT PROTEIN SHR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28228:SF1">
        <property type="entry name" value="SECRETORY COMPONENT PROTEIN SHR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08229">
        <property type="entry name" value="SHR3_chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029187">
        <property type="entry name" value="Shr3_AAP_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00786">
        <property type="entry name" value="SHR3_chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="23979" checksum="708B5F67F3CB2832" modified="2011-11-16" version="1">MVTYRDIVPIGTGLIIAASSFGLGVVYSSWPYDVNTLWRYEEGAIDVSIAHYQQWANSPMYIHYTLQAVAGLGLLGCFIKLYKPNEDAKYFEYGTLGLLMVGLIIYLTNLRTGINSCITGNWGEVDAATGVNVMAASQVMIVFALVGVLVLQAGLYYAEWYENKLKEEFYKEEAAEAAAAAEKQAKEEEEVQAETQENAETSGAARKTKQTARKRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH57</accession>
    <name>G3AH57_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_53729</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372899.1">
        <property type="nucleotide sequence ID" value="XM_007372837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH57"/>
    <dbReference type="FunCoup" id="G3AH57">
        <property type="interactions" value="112"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AH57"/>
    <dbReference type="GeneID" id="18871808"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53729"/>
    <dbReference type="eggNOG" id="ENOG502QUGB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016668_2_0_1"/>
    <dbReference type="InParanoid" id="G3AH57"/>
    <dbReference type="OMA" id="ENHSMFC"/>
    <dbReference type="OrthoDB" id="410044at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12453">
        <property type="entry name" value="RRM1_RIM4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034352">
        <property type="entry name" value="Rim4_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050907">
        <property type="entry name" value="SRSF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23147">
        <property type="entry name" value="SERINE/ARGININE RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="106"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="300"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="523"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="523"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="636" mass="70994" checksum="9A244C83F8170688" modified="2011-11-16" version="1">MQSKQSRKPDTQTVSSSCESFSSTNIEAIEAVESGNSPRKNINSLYSTVGANHVDTSSVSSSLGGDDISSEDSDSENSQVLSGKELKSVESLSEGDHDIIRGRPSACVFVASLCSNKTDDELCISVTEHFQQWGKLATVKVLRDTCNRPYAFVQYTKDEDSKLAIEEGHNSLLDGRNIRCEAAKVNRTLFISSKSFLTEELIRERLSAFGEIEELVPSNSKGEIFSSGIASRGYRNWFCKFVYRDDAIRAFANLTEENLYRVDWAQNIDKSNARNHVTFQYSSHSDDNDENLEKLKFDKYSVFVGHLNSEVEENELQDRFERHGTISEMNLVKKPNNTFAFIKFKKESSAASAVERENHSMFCGKTMHVQYREVHSPVAKTTPFGVALAPPPINLTKRLSATPSRATEGSRFHTSKTQFEISKPKFNHYPSNTFNKSFNRIGGKYSNGKHYNSFKRSYGSGYNNSGAYGGSNYNPSKPGSKSFDSKKNDYYTWSASNPTQLGLHGESPFKGYPGISRDISDFKSKRSLDGKSEASGSRGSGNKYDHKAMNNNSRGSNINNSGYPLFYYFPNNDLMANFAAGSSSGPAPYYNIYQQYYPTYDPSDYPPTSGTHASFAIPNYMYYPSESEIASNEKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH73</accession>
    <name>G3AH73_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA (guanine(37)-N1)-methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA methyltransferase 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TRM5</name>
        <name evidence="12" type="ORF">SPAPADRAFT_58742</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="10">Predominantly in the mitochondria and in the nucleus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRM5 / TYW2 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="10"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372915.1">
        <property type="nucleotide sequence ID" value="XM_007372853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH73"/>
    <dbReference type="FunCoup" id="G3AH73">
        <property type="interactions" value="1127"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AH73"/>
    <dbReference type="GeneID" id="18872582"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58742"/>
    <dbReference type="eggNOG" id="KOG2078">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022610_2_2_1"/>
    <dbReference type="InParanoid" id="G3AH73"/>
    <dbReference type="OMA" id="VGSHSQF"/>
    <dbReference type="OrthoDB" id="408788at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070901">
        <property type="term" value="P:mitochondrial tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.110:FF:000001">
        <property type="entry name" value="tRNA (guanine(37)-N1)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03152">
        <property type="entry name" value="TRM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025792">
        <property type="entry name" value="tRNA_Gua_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF36">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="11">
        <location>
            <begin position="147"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="274"/>
            <end position="275"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="302"/>
            <end position="303"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047783"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_03152"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW35503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="474" mass="55041" checksum="C3304EA33AD4E5FA" modified="2011-11-16" version="1">MNFQRLVHFGKKLRVLMISEVKKYPPPVNRSMVELDRSFFHREVPLLAAYFPNAKHIGEFVKRCKQDILYMPNIKHIVQMDDSKAVLLQDRITDVSQLSALSQEKVKEFGVELRPYTMQLDYSFWKTDDILRAILPENLLDETPSGFSQAGHLAHLNLKNEFKPYGKLIGQVILDKNPSIRTVVDKADTIANKFRTFQMNLLAGEDNFLVEQSESGCKFRFDFSKVYWNSRLSTEHDRLISKFAKGDVVGDVFAGVGPFAVPAGRKEVIVLANDLNPESYKYLQENITLNNAGLFTRAFNLDGREFIRQSPKLLYDLYTKTPVIQQKRIVRRKVPAEEPGQPAKSVKEEKIIEMKVPKFYKHYVMNLPDSALTFLDEFVGLYGRYPEIAQDLKQDPEFQLPMIHVHCFEKFEIDEEPPMEELHRRVYDKIVRLMGYELDFSKMEFHHVRQVAPTKPMFCVSFELPEELAFKVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHC6</accession>
    <name>G3AHC6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Long chronological lifespan protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_59512</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LCL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373675.1">
        <property type="nucleotide sequence ID" value="XM_007373613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHC6"/>
    <dbReference type="FunCoup" id="G3AHC6">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHC6"/>
    <dbReference type="GeneID" id="18872950"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59512"/>
    <dbReference type="eggNOG" id="ENOG502S416">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142363_1_0_1"/>
    <dbReference type="InParanoid" id="G3AHC6"/>
    <dbReference type="OMA" id="KPATHDE"/>
    <dbReference type="OrthoDB" id="2234316at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23996">
        <property type="entry name" value="LCL2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034543">
        <property type="entry name" value="LCL2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38425">
        <property type="entry name" value="LONG CHRONOLOGICAL LIFESPAN PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38425:SF1">
        <property type="entry name" value="LONG CHRONOLOGICAL LIFESPAN PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="115" mass="12671" checksum="C3841FE5ECAB73BC" modified="2011-11-16" version="1">MFDFFNFNQGGGGGGGQQARSPQDNENVLLNTRCEKYLCPDTGVCAKSPRFCPCPFPSSQLKCPLPNGNYICVSKPAGEEIADKYSDPNTNWKIDAKNNDIRDCGWVNRAYKGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHN4</accession>
    <name>G3AHN4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">V-type proton ATPase subunit H</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_59630</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the V-ATPase H subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373782.1">
        <property type="nucleotide sequence ID" value="XM_007373720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHN4"/>
    <dbReference type="FunCoup" id="G3AHN4">
        <property type="interactions" value="448"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHN4"/>
    <dbReference type="GeneID" id="18872996"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59630"/>
    <dbReference type="eggNOG" id="KOG2759">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025709_4_0_1"/>
    <dbReference type="InParanoid" id="G3AHN4"/>
    <dbReference type="OMA" id="HSGHLRW"/>
    <dbReference type="OrthoDB" id="10263554at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.150">
        <property type="entry name" value="V-type ATPase, subunit H, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004908">
        <property type="entry name" value="ATPase_V1-cplx_hsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011987">
        <property type="entry name" value="ATPase_V1-cplx_hsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038497">
        <property type="entry name" value="ATPase_V1-cplx_hsu_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10698">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10698:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11698">
        <property type="entry name" value="V-ATPase_H_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03224">
        <property type="entry name" value="V-ATPase_H_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032184">
        <property type="entry name" value="ATPase_V1_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase V1 complex subunit H C-terminal" evidence="6">
        <location>
            <begin position="352"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR032184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="478" mass="54104" checksum="F7D5899A03BE99D0" modified="2011-11-16" version="1">MTSEFTPLVIDSTFFADSKKIVKERIIPWEGLARSGVISDDDAGHMKTLGKQVQENRNKTVLAQLDLYSNSILNILNRLNVNEKEDIVKHVLALTNDLLLEIPEFVDTLLKFSSIDNSLPYYPFIKHLANSDNFIKTLGLYNLTILLTKASKDSSISAKIDDEIIIKIFDILSSPNFIGGIDSNYQFIGIQLLQELLIVKQFKTIYQNNNLVSNFKPINQLITRSATHPNATGLQLSYNILLATWILSFSAPINRVLLTSFPQLAGCLFTIAKDSIKLKIVRISVGILKNFVAVCTNSHEQFKVIKLLLFHDALNTIRTLQERKFASNGSDEELSNDLAYLSDNLQEVVTSKLTSFDEYMTGLENPKLISWASPTHKSVEFWLENSGKFKDSNYKLIKRIFEILITNTSDNSTINVILLNDLQFLIKNLGQELISFINTEKGGQYKLLIMSYLENNYGNNELKYEALKTIQLLVGHTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHP9</accession>
    <name>G3AHP9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_134196</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373797.1">
        <property type="nucleotide sequence ID" value="XM_007373735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHP9"/>
    <dbReference type="STRING" id="619300.G3AHP9"/>
    <dbReference type="GeneID" id="18869788"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134196"/>
    <dbReference type="eggNOG" id="KOG2130">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016785_1_2_1"/>
    <dbReference type="InParanoid" id="G3AHP9"/>
    <dbReference type="OMA" id="WPAYKNW"/>
    <dbReference type="OrthoDB" id="424465at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050910">
        <property type="entry name" value="JMJD6_ArgDemeth/LysHydrox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480">
        <property type="entry name" value="ARGININE DEMETHYLASE AND LYSYL-HYDROXYLASE JMJD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480:SF21">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="57"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="2">
        <location>
            <begin position="270"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="557" mass="63821" checksum="EF913BA7C6A45DA8" modified="2011-11-16" version="1">MTTDSEPKRRKVYVYPNNPRSISVFSVSGRHPLNVKPSGNSLLNDDIALTKVRDALLGAFASFPDELIQEVLSYVDDVESLRSLAHTSRIFYAFLYDEELWKKLYVKDITMYTHQPWLGSWRNTVLGIHDSADIQLQDNLLCSDVLYRPFQCSQINYAKVFTKILHEEETYHHDSLLGQLGKLPQGRILRINEADLSITDFNTNYHDTPFILTNSDPQRWPQWTFPQLIKEYSDVKFRQEAVEWDLNKFSQYLQNNKDENPLYLFDCNSIAMQTLRKEYTPPAIFQQDLFTVFTKEEQFTCRPDHAWLIMGSARSGSTFHKDPNYTCAWNVAITGRKLWIMLPPHITPPGVSTDDEESEVTSPVGIAEWVLSGFFNDSLKIAECVIGVTFPGECMYVPSGWWHSVINIDDSIAITQNFVPISKLANALDFLKNKQGQVSGFRPREINSTLHYLLKHGADEPSFQKYVDVFDSLNIDVNEDCGEIADLPAMPIFELFGYLLKLNGMESQLDEALVKLQKIELKAYEKENGKSKAWERLTEPASNSAFSFGFDESSDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHR6</accession>
    <name>G3AHR6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59661</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373814.1">
        <property type="nucleotide sequence ID" value="XM_007373752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHR6"/>
    <dbReference type="GeneID" id="18873012"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59661"/>
    <dbReference type="HOGENOM" id="CLU_2251735_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHR6"/>
    <dbReference type="OrthoDB" id="4026687at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="104" mass="11507" checksum="A9927DE935982983" modified="2011-11-16" version="1">MGGASSDLQSDIGSSINQDKRDSVSTSSSDSIDKLAFVTGFYQHRSYKRGDDDKEENGSGETDIEGNTMSDYNHQSYSDLTDMDGYEERPRLYLANPDNDSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHS1</accession>
    <name>G3AHS1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">endopeptidase La</fullName>
            <ecNumber evidence="7">3.4.21.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_70378</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase S16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="7"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373818.1">
        <property type="nucleotide sequence ID" value="XM_007373756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHS1"/>
    <dbReference type="FunCoup" id="G3AHS1">
        <property type="interactions" value="1007"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHS1"/>
    <dbReference type="MEROPS" id="S16.010"/>
    <dbReference type="GeneID" id="18875322"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70378"/>
    <dbReference type="eggNOG" id="KOG2004">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004109_1_0_1"/>
    <dbReference type="InParanoid" id="G3AHS1"/>
    <dbReference type="OMA" id="VEWYQEV"/>
    <dbReference type="OrthoDB" id="2411602at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19500">
        <property type="entry name" value="RecA-like_Lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000021">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054594">
        <property type="entry name" value="Lon_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
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        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
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        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718">
        <property type="entry name" value="LON PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718:SF2">
        <property type="entry name" value="LON PROTEASE HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF22667">
        <property type="entry name" value="Lon_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 8" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="12">
        <location>
            <begin position="137"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="11">
        <location>
            <begin position="840"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="64"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="740"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="703"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="753"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="934"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="977"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1061" mass="118248" checksum="78D1E8DBB53AD03E" modified="2011-11-16" version="1">MIRRGSKLRQVVRTPNQLRSTLVGLRTATTVVSHQQLPISYSIPTILPSISTPGPQFQIPHSILLESSKEESTEDTPDKKPKEKKERAPRKKKENSSAAPPATPPASPPSDNFDKDPEDSNESTKSPGVDEETGLYPPMIAINFMHRPPLPGSTFHISVNDPEMLRAIAAIEEKREPHFVLFRARDPDQPDTDSIDDKESVHDIGVLCQVLRTHTTDSRLTLVGFAHKRIKLATLIPPKTKSTHLVNSYLKDYNISTAMVEPAEDKPFEKTSPEIVMLVDGIKSILAEIQQANPGSKNSLPLTPQMLEDPSLFADYIAGKLAVPADKAQELLETLDVETKLIKVIDLAKVELEASLLKMGAVRELSKRAEKNQTNAFIKEFISELRKKIGDTSSSKSTKFEERIKKNKLKLTDEAKEAYLNEKEKLATAGPADMEGSMHERYLDWLTSIPWGVYSKDSFNIKKAKEILERDHYGLKDVKERILEFISRGKVSGNVEGKILCLTGPPGTGKTSIAKSIAEALNRRYVRIAVGGLHDVHELKGHRRTYVGSIPGRIIYALKQAKTSNPLMLLDEIDKLDLTSRSGGGGASALLEILDPEQNNGFVDNFIEVKVDLSKILFVCTSNYIENIPAPLRDRMEVIDVSGYTKNEKIEIATKYLIPKESKKVGLDETRVVIPRETVDRMIDKYCRESGVRNVKSLISRIFNKATLKIVEQIEEREEAEKEKNDKEIVIEAKDVETKPITETVEPELEEALETKSVESEHNKETQPEEKTEQKTEAESEKVETKSEKVGTEEPEIETVDTTTSEKLQIPDDIKLEITPDKLKDYIGPEIYTRERVYETLPPGVASGLAFTSSGNGEVLYIESILTNSIASGTGHPGIRVTGRLSEVMKESASIAHAFAKQYIVKQFPENRFFEAAEIHLHCPGGAVPKDGPSAGVAFTSCIMSLALQRSLPANVAMTGEITVTGKVLPVGGLKEKTLGAKRYGCDTIIYPKDIQNELEEMPAEVKEGVTFIPVEWYQQVFDVLFPGLTQQEGNDVWKEEFKKLEEKKNKKKDQEKESDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI18</accession>
    <name>G3AI18_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_54484</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007373854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI18"/>
    <dbReference type="STRING" id="619300.G3AI18"/>
    <dbReference type="GeneID" id="18871867"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54484"/>
    <dbReference type="eggNOG" id="KOG1492">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1175265_0_0_1"/>
    <dbReference type="InParanoid" id="G3AI18"/>
    <dbReference type="OMA" id="NYEIWTC"/>
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    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46156">
        <property type="entry name" value="CCCH ZINGC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46156:SF1">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="CCCH zinc finger"/>
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    </dbReference>
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        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="5">
        <location>
            <begin position="46"/>
            <end position="72"/>
        </location>
    </feature>
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        <location>
            <begin position="79"/>
            <end position="107"/>
        </location>
    </feature>
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            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
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        <location>
            <begin position="46"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="79"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="232" mass="27190" checksum="714F5A68A948D22D" modified="2011-11-16" version="1">MVNLGFCQKGSTCKYIHDKNKIKICPLFLSGKCFNRNCLLSHSCNDNNTAMCRYFLEYKCHNSNCKYRHMKPPHYDDPNYEIWTCRPFAIGGWCPRGKRCPFLHLPNCPDFEENGYCSRKQECPFNHQVTLRTQEQISTRSNKYIREEVEVETQKDDKPEKIIISSYTVDPEVLFINDTTSNYQYYIDNTAQELRSKEDAPSSEFLIEISDTESEFSLDEDQLEGNDDYVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIC8</accession>
    <name>G3AIC8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Processing of GAS1 and ALP protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59831</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373982.1">
        <property type="nucleotide sequence ID" value="XM_007373920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIC8"/>
    <dbReference type="FunCoup" id="G3AIC8">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIC8"/>
    <dbReference type="GeneID" id="18873093"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59831"/>
    <dbReference type="eggNOG" id="ENOG502S88B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150165_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIC8"/>
    <dbReference type="OMA" id="FGWGNKT"/>
    <dbReference type="OrthoDB" id="4227028at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011431">
        <property type="entry name" value="Trafficking_Pga2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28199">
        <property type="entry name" value="PROCESSING OF GAS1 AND ALP PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28199:SF1">
        <property type="entry name" value="PROCESSING OF GAS1 AND ALP PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07543">
        <property type="entry name" value="PGA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF022909">
        <property type="entry name" value="UCP022909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="15087" checksum="630974C5C9F03A3C" modified="2011-11-16" version="1">MFEEIFEFVDKETFTKYFRLTIVVCAYLIFRKYYSQWAAKKQTERQMADDEKEKAEKPERERKAREEQAQQLEAEAKQFGWGKKTRRTVKTTEAALDQIIQEQRQRNQTSYDAQEDADIEDLLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIQ3</accession>
    <name>G3AIQ3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59892</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374053.1">
        <property type="nucleotide sequence ID" value="XM_007373991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIQ3"/>
    <dbReference type="GeneID" id="18873123"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59892"/>
    <dbReference type="eggNOG" id="ENOG502RXV1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_781121_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIQ3"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="355" mass="38588" checksum="066E5F9DC4C12B1C" modified="2011-11-16" version="1">MNLPQGTPSSLTPRNSISQGSPLVQPHPPRTSISSTASLSRSGSISGIPPISVPSNSTGLPTMSFRNNSIIQTIRDDRTSISSLARVSVSSLPSKSMNIPHHQTTNNALAHMPVLFGSTGGSISGPLSRNPSLSAPSGAPGANSGIQQNNVSSLRNSSIVSANSGYYSRSGSVVIPYNADKEEPLKIDKDKLDDHNKKLNKKKIKTIHHNHRTTTKTSGDSKGKSATPNPPPLEIPWTMEEDELLINRRNRELSFAELSILLPQRTEGEIWARIDHLEKLRNGAHRSATSSDPRRAGIDFYDDDDDDVIGGLSDDEDDDDDDDDNNDVLSVRKRKRRASSAVNPLSVRETIRKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIY7</accession>
    <name>G3AIY7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_48914</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373382.1">
        <property type="nucleotide sequence ID" value="XM_007373320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIY7"/>
    <dbReference type="STRING" id="619300.G3AIY7"/>
    <dbReference type="GeneID" id="18871285"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48914"/>
    <dbReference type="eggNOG" id="KOG2039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005966_1_0_1"/>
    <dbReference type="InParanoid" id="G3AIY7"/>
    <dbReference type="OMA" id="ARCADHH"/>
    <dbReference type="OrthoDB" id="10023235at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031332">
        <property type="term" value="C:RNAi effector complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000018">
        <property type="entry name" value="Serine/threonine-protein kinase 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016685">
        <property type="entry name" value="Silence_cplx_Nase-comp_TudorSN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302">
        <property type="entry name" value="EBNA2 BINDING PROTEIN P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302:SF2">
        <property type="entry name" value="STAPHYLOCOCCAL NUCLEASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017179">
        <property type="entry name" value="RISC-Tudor-SN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TNase-like" evidence="6">
        <location>
            <begin position="150"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="6">
        <location>
            <begin position="313"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="5">
        <location>
            <begin position="710"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR017179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="894" mass="99031" checksum="B7E1B6819FBF8AF3" modified="2011-11-16" version="1">MSSFLVAKVKNVLSPDTVVLIPPKTTQCPAPERLLTLSYVRGGDDFESKEFVRNLLIGKDVKFKVLYKATNREFGDIQSPIFKSLIEYLLKNGVVKLKENFSESEGDIYYELSEAENQAKLNQVGVWGKQQEKIDLVALDEKIIAKSQKYPLKLVVEKVISGDRIVGRIIVNKNQHVSTSLLLAGVKSPRTDDPQQPANVTKVGHQAKQFVEEKLLTTKAELKVSIIGESQNGIPIATVIHPSGNNIHEKLLEAGLGEVVDWQSTLIGSSTMVTLRRAEQTARALGKGLFANAHTTSAKPAISASKSLKPGATLEGVTVAKVVGADTLVLRLPHSEEEVTVQLASIRAPRLNDTTVTTDSQKQHALVLTAKEFVRHQVIGKTGTVYIDGYRNENKDLGLEARFLISFKYGNTDLSELLVTNGFATVIKHNKATQHERSLNWDKLIELEEEAKKSSKKGIYGDLNKVLTVSPRIIDASENFTKAKTFFNGFKQKGRISGYHVEFVSSINRVRLFHPREGLKLTLILGGLSNDKSEALGEEGLKLLNKRFLQRPVEFEIYDTDKLGGFIGNLYANANSLAPIQVSLLEQGLIKVHDFAVNANPLASKLLQAEEGAKSAKKGIWAGYDPAKVEHELQASNEKLQQLNLDASKPQFFDIEVVDVDSTGVISFQLLDATTKQKFTQFKNSFSQFHAQPASASTNSPDLPYNLTRGPKKNELVSAKFSEDNKYYRARVINFDKPSNKYEVKHLDYGNVDKVPLSALRALPAKFNLTTIPAFARTTTLQNLRLPPSDYLTDAIYALEDLVFDKKLVISALPGTAAEYEGILYDSEQSLKDSSYTINKQLVNKGWAIVDTKMVKPAVKSYVDELIPVQNEAKARHLGCWEFGDVSFDDEEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ33</accession>
    <name>G3AJ33_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1,3-beta-glucan synthase</fullName>
            <ecNumber evidence="3">2.4.1.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">1,3-beta-D-glucan-UDP glucosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">GLS2</name>
        <name evidence="13" type="ORF">SPAPADRAFT_54663</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;3)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;3)-beta-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21476"/>
            <dbReference type="Rhea" id="RHEA-COMP:11146"/>
            <dbReference type="Rhea" id="RHEA-COMP:14303"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37671"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.34"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 48 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.34" evidence="3"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374129.1">
        <property type="nucleotide sequence ID" value="XM_007374067.1"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ33"/>
    <dbReference type="GeneID" id="18871879"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54663"/>
    <dbReference type="eggNOG" id="KOG0916">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000844_0_1_1"/>
    <dbReference type="InParanoid" id="G3AJ33"/>
    <dbReference type="OMA" id="IKHCEYM"/>
    <dbReference type="OrthoDB" id="1880850at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000148">
        <property type="term" value="C:1,3-beta-D-glucan synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003843">
        <property type="term" value="F:1,3-beta-D-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006075">
        <property type="term" value="P:(1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051278">
        <property type="term" value="P:fungal-type cell wall polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026899">
        <property type="entry name" value="FKS1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056261">
        <property type="entry name" value="FKS1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003440">
        <property type="entry name" value="Glyco_trans_48_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741:SF97">
        <property type="entry name" value="1,3-BETA-GLUCAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741">
        <property type="entry name" value="LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14288">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23605">
        <property type="entry name" value="FKS1_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02364">
        <property type="entry name" value="Glucan_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01205">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="324"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="464"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1131"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1222"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1248"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1337"/>
            <end position="1359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1379"/>
            <end position="1409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1416"/>
            <end position="1439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1451"/>
            <end position="1470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1482"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1519"/>
            <end position="1538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1572"/>
            <end position="1596"/>
        </location>
    </feature>
    <feature type="domain" description="1,3-beta-glucan synthase component FKS1-like" evidence="12">
        <location>
            <begin position="90"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1637" mass="189464" checksum="AFE8B5A0292B3FE8" modified="2011-11-16" version="1">MFDYLMRLLDSRASRLGPMQALRSIHADYIGGVNSNFRRWYFAASLDIDDAFGLDNITKSGKVKDSNEVVATLEQAEESWSSNMLTLSPTDCTIQLALFLLVWGEANNIRFMPECLCFIFKCCNDYYFSIDPDVPVEPVTVSFLDHIITPLYNFYRDQSYVLIDSKYHRRDKDHDSVIGYDDMNQLFWYSKGLERLVLTDKKTKLMSLQPSERYEKLNQILWHKAFYKTFRERRSWSHVLVNFNRVWIIHISVFWYYTLFNSPTLYTKNYQQVLDNQPTTQARLSVLSLGGSIAILMCMASLLFEFSLVPRKWTGAQPILKRLLLLFLAFIVNTGPTVYLFLVYPLDVENTLGLVLSSFQFGFSVIMVLYLSIAPLGKIFTSSRKQDRRFLATKYFVTNFYTLTESDRIASYGLWFAIFISKFIESYFFLTLSMRDPVRELSVMKMTRCAGEVWIGNWLCQRQTVIVLCLIYLTDLVLFFLDTYLWYIVWNTIFSVCRSFYIGVSIWTPWRNIFSRLPKRIFSKIISVSGDRNVKAKLLVSQVWNSIVISMYREHLISLEHVQKLIYKQIDTPGVEGGSILKEPIFFVSQEDQSIKSSLFRDQSEAQRRVTFFAQSLSTPMPEVGPVHLMPSFTVLIPHYGEKITLSLREIIREEEQYSHVTMLEYLKQLHPLEWTCFVKDTKMLAEEFETDSSSAECKKEKLDDLPYYSVGFKVATPEYILRTRIWASLRSQTLYRTISGFMNYSRAIKLLFDVENPDGFDSEQEKLEQASVMAHRKFRIITSMQRLKYFSPEEKENTEFLLRAYPELQICYLDEVVDDVTGEIVYYSALVDGSCAILANGEREPKYRIKLSGNPILGDGKSDNQNHSLIFCRGEYIQLVDANQDNYLEECLKIRSVLAEFEEATFPLDPYAKDLKNTEMAYPVAIIGTREYIFSENIGILGDVAAGKEQTFGTLFARTLAHIGGKLHYGHPDFLNGIFMTTRGGVSKAQKGLHLNEDIYAGMNVVLRGGRIKHCEYMQCGKGRDLGFGSILNFTTKIGAGMGEQMLSREYFYLGTQLPLDRFLSFYYAHPGFHLNNVFIMFSIELFLLVCANLAALTNESTICEYDRFRPITDPRRPVDCYNLIPVVQWLQRCIFSIFIVFVISFVPLGVQELTERGFYKAITRLGKQFASFSPLFEVFVCRIYGYSLVSDISIGGARYLATGRGFATIRVPFSTLYSRFAAESLYFGGFCGLLIFYSSISMWKISLLYFWITIVGLLICPFLYNPNQFSWNDFFLDYKEYLKWLNRGNSKPRISSWINFTRLKRSRLVGVKSKRYSINEEIKVVSEVKPSRFKLVISETLQQFVVISLVGFAYLFANSQNDARGTYPVNSILRILLVSFGPIVVNVAILIVFFVLSVITGPIFTLFCKKYPSFLASGAHALAVINHVFFFELLWLLQNWNFSRTLLGLALSILLQSWFLQTLTILFISREFRHDRSNRSWWSGKWATAGLGWYIITQPLRETICKLTEMTYFAGDLVASHVILYAQFPILFIPYVDKWHSLMLFWLKPGNQLRPRILSKRQKRKRRFQANLYLLIFLLSFVAFASIFVVPIVANKYFDIDYDSYMPGVLQELVQPYVIASSRKGLLKNLEVQHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ55</accession>
    <name>G3AJ55_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59173</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373396.1">
        <property type="nucleotide sequence ID" value="XM_007373334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ55"/>
    <dbReference type="STRING" id="619300.G3AJ55"/>
    <dbReference type="GeneID" id="18872805"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59173"/>
    <dbReference type="HOGENOM" id="CLU_1890836_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ55"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="135" mass="15910" checksum="9AEB4897AE4DD375" modified="2011-11-16" version="1" fragment="single">MSFNNHSHLRPNIPTYSQHSDDEEQYTDHNSPLTGNPYFQNSAPSRSFNNSTTSLNFQHPVPPPTYDTSSHSIIDDYYNRPIFDTNSRLTAQENPFHENYEMEYTGQGYNNNYNQAFVPNVYDDEEREFDQHIQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJA1</accession>
    <name>G3AJA1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trehalase</fullName>
            <ecNumber evidence="5">3.2.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Alpha-trehalose glucohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_70652</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:32675"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="3.2.1.28"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glycosyl hydrolase 37 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.28" evidence="5"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374144.1">
        <property type="nucleotide sequence ID" value="XM_007374082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJA1"/>
    <dbReference type="FunCoup" id="G3AJA1">
        <property type="interactions" value="332"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJA1"/>
    <dbReference type="GeneID" id="18875340"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70652"/>
    <dbReference type="eggNOG" id="KOG0602">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006451_1_1_1"/>
    <dbReference type="InParanoid" id="G3AJA1"/>
    <dbReference type="OMA" id="WLFMMTK"/>
    <dbReference type="OrthoDB" id="3542292at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004555">
        <property type="term" value="F:alpha,alpha-trehalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005993">
        <property type="term" value="P:trehalose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000026">
        <property type="entry name" value="Trehalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001661">
        <property type="entry name" value="Glyco_hydro_37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018232">
        <property type="entry name" value="Glyco_hydro_37_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011120">
        <property type="entry name" value="Trehalase_Ca-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23403:SF6">
        <property type="entry name" value="CYTOSOLIC NEUTRAL TREHALASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23403">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01204">
        <property type="entry name" value="Trehalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07492">
        <property type="entry name" value="Trehalase_Ca-bi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00744">
        <property type="entry name" value="GLHYDRLASE37"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00927">
        <property type="entry name" value="TREHALASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00928">
        <property type="entry name" value="TREHALASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Neutral trehalase Ca2+ binding" evidence="7">
        <location>
            <begin position="294"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="68"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="81"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="951" mass="108685" checksum="A65A870E05B9F78C" modified="2011-11-16" version="1">MTYFLPQPEASRMLATSSMSSEEDPYDTAENYYGDARKVNKLTSPLARVRTYSVFESKTTRQDFLKQLLAEVSHNPPPSPHVEHPDTIRETSEPPSSGSSSEVPSKSPTPQIQFNTPGEKAYDHRKIQFAIHDSEDEEDSEDEDPVSAPSNGVIKDDDAPPLSSTTPPIAQVHHAPLTSPALMTANIDAHRNAEPTHAPPPVSPRVRRGGATGLHDIVPVDEMESEDLPPNIKVPDFSGLTRKPSIIAIYQDNSSSESVYDKAEKNKRRYRRQSVDDTTIRPKKFYIPDVEDTLKELLANEDTDGNCQITIEDSGPKMLRLGTANSNGYYQSSVRGTYMLSNLLQELTIASRFGRRQILLDEARLNENPLNRMKRFISTAFWKALTRKITKENVTEMSADTKILETWIDDDGVLHKDEPSHRIYVPYNRADQYEFFKRIQKDHKNLDVQYLPKVIDAQYIKSINKKPGLLALATRPDPDDPENLLSWPYVVPGGRFNELYGWDSYMETLGLLTDVTPGRREHLELARGMAENFIYEIQHYGKILNANRSYYLGRSQPPFLTDMALRIFNKTIEIAPDEIDDATDFLKRSTLAAIQEYETVWCCKPRLDERTGLSCYHPDGVGVPPETEATHFDAVLKPFLEKHKLSRDEFIEKYNDGIIKEPYLDEYFLHDRAVRESGHDTSYRLEGKCAYLATVDLNSLLYKYENDIAFILANFFHDNLEDYDGTIHTSPIWKERAKQRRINVDKYLWNEEDSIYYDYNVHDQEQTRYESATTFWPLYAELSSPEQAEALIRNSLSKFEEHGGLASGTLKSRGEVGLTRPSRQWDYPFGWAPQQILAWIGLVNYGYDGVARRLAYRWLFMMTKSFVDYNGVVVEKYNVTKGAVPHRVDAEYGNQGLDFKGVATEGFGWVNASYLFGLQFLNLYAQRALGTLTPPKVFLRNMHPSHIKEFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJD5</accession>
    <name>G3AJD5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glycosyl hyrolase, family 3-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_133385</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374178.1">
        <property type="nucleotide sequence ID" value="XM_007374116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJD5"/>
    <dbReference type="STRING" id="619300.G3AJD5"/>
    <dbReference type="GeneID" id="18869742"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133385"/>
    <dbReference type="eggNOG" id="ENOG502QTNX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008392_3_0_1"/>
    <dbReference type="InParanoid" id="G3AJD5"/>
    <dbReference type="OMA" id="NVSHACL"/>
    <dbReference type="OrthoDB" id="4215304at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009254">
        <property type="term" value="P:peptidoglycan turnover"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050226">
        <property type="entry name" value="NagZ_Beta-hexosaminidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30480">
        <property type="entry name" value="BETA-HEXOSAMINIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30480:SF8">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04060)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 3 N-terminal" evidence="5">
        <location>
            <begin position="23"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="428"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="430"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1030" mass="114833" checksum="ABDD294EE6109206" modified="2011-11-16" version="1">MDSPDMSKLNVGQLLIGGFQGTTVTPQAYHLIVEHRVSTMFLSRKNAVNVEQMTKLIKDLQYIAYSQGHKYPILFAMDEEGGMMNSLFDPDFLTQYPGAMALAATGDPQLVYEVSKAVAIELKKIGFSIILGPVLDVVTKLSHQLVGVRSFGTTVEDVVKYGMMCAKGLQDGGLFTVGKHFPGIGNATVDSLLELPMIGDSLDQVKNFNSVPFAKLIEQGVLDGVSAAGCAVPNISPDETHACLSPMIINQLLRNELKFDGFVVSECLEMETLYHSIGLGQGVVLAISAGCDLVMVCHDLALQNEAVESMRKAVHNGDLDEEIILASIARIEKLQKKSPTWSDLFPRGEASAKEDKISLFKYEYPELWQHHQKLSSMAYQKSITLVRDFNGTLPISKFLAPLNENESGEQQVDSILLLTPLLNPIYQVNPEHDHDKEKDDDSSTRSPSPGNKHGQPRLFTGEEVFQKFGDLLANHPINKSKSYNVLHTTYTANGLTPLHESLIESSKIVIVLTSEASRNMYQIGIVKYVSILCGANPASFSKGGQATYSQLTKPLIIVATSSPYDFFYSRSIGSVYMCCYDYTNNALEKLVGAIMGDFDPEGCIPGEKKFLIRKRRLTDELNSPPSSIKAPPGKVTLPKRRWLVDELDLKRDWTSLVKLWKTNTGEVSGYNENKNKIDYQAERFYKRLYGLLATTAKQQKHFVVRNSSLNILYGVVLTWADETPVSNDLTGDGHELPKTGHILFILVDKSKRLQSVGKNLHARAIRYLIKEQGCTSIKLGGSFPLIMAHDNSNLNNSRVRAFMNGVGWNLSSEGQRSRKKKIMIIRNLENWVVPKKIFHELMIVGVRFDICNDSEKLMTLIESSINDNLDDEDACLNKSNLYLEAIKHIRNQSPLGVKIIIALEPTHQKVIGSIIFFTNKSQFSKFYPFIDECVDTSNEKDIPLTGGIIGPVIDPSYSNLADIFKYGLICSAITFLKSSMNEDSQIDQCVMVDVLDDKTISGVKEIGFDEWKSYYDYYDKRTNPEKVFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJL6</accession>
    <name>G3AJL6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC transporter TMD0 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_134077</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373501.1">
        <property type="nucleotide sequence ID" value="XM_007373439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJL6"/>
    <dbReference type="GeneID" id="18869780"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134077"/>
    <dbReference type="eggNOG" id="ENOG502RQGR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1182646_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJL6"/>
    <dbReference type="OMA" id="ISIFWME"/>
    <dbReference type="OrthoDB" id="10433409at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056227">
        <property type="entry name" value="TMD0_ABC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24357">
        <property type="entry name" value="TMD0_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter TMD0" evidence="3">
        <location>
            <begin position="19"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="235" mass="27186" checksum="C2A636FD597F7B4C" modified="2011-11-16" version="1" fragment="single">MLDLQAYPELTCGLRAILYPMAPNNDNAFNPCFLTLLLTLFGVGFAIYGGITFYLTLKRPRYGDLLPSSTGMSHYIRLNSVLLQCLLMFYLESFLSIHERLADQKLLSFTIVNLGLVCVILPLHVIEVMYEPIPCDVLVLYWPFLTLLELALYFQDNYTGWRIIKSIEYDSTIQIVEALLILNSMLIFVLEYSREPTQELIAHYTETDPKKLSEPNVVQRITFSWMNELIMNSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJS1</accession>
    <name>G3AJS1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="3">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_133766</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="3"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373556.1">
        <property type="nucleotide sequence ID" value="XM_007373494.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AJS1">
        <property type="interactions" value="860"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJS1"/>
    <dbReference type="GeneID" id="18869765"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133766"/>
    <dbReference type="eggNOG" id="KOG0902">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000893_1_1_1"/>
    <dbReference type="InParanoid" id="G3AJS1"/>
    <dbReference type="OMA" id="TIEVWQS"/>
    <dbReference type="OrthoDB" id="10264149at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061909">
        <property type="term" value="P:autophagosome-lysosome fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140504">
        <property type="term" value="P:microlipophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05167">
        <property type="entry name" value="PI4Kc_III_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.70:FF:000011">
        <property type="entry name" value="Phosphatidylinositol 4-kinase alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000022">
        <property type="entry name" value="Phosphatidylinositol 4-kinase stt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000014">
        <property type="entry name" value="Phosphatidylinositol 4-kinase STT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045495">
        <property type="entry name" value="PI4K_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF15">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19274">
        <property type="entry name" value="PI4K_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIK helical" evidence="9">
        <location>
            <begin position="1341"/>
            <end position="1525"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="8">
        <location>
            <begin position="1598"/>
            <end position="1874"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1890" mass="215788" checksum="ECE8E95B56616F1A" modified="2011-11-16" version="1">MDFAGISRGSIRDQALRKLAELTVNYDASTLKEISPRISKPDFSKLLSSTPKEYSKNKSMSHVAISLKEYQVLLSLCESTGLAFKSKDQCKELIDKFKAYLLELPDQQFAYNILSQAVTISPWALLGEKLTMALINLGFMYGELYMEDVIDIFHEFIEGFFYTAEPQLSNFLSLSGVLQGLATNARFLTVSPRAFKVFTMLDSAIDSEEFLTDVESYCEELAEEPPQEYDTVLNRDYFVNFSPIMYLELLAKIMCQIINAIIENKNNSLLHCILSKVASKYDDVDESSNGTLFTPNLNFSRYQMNIIKTLSDLALQKLEFLDRGEPYIVYSSFKRLKLGYLAKSHNLQVLSCGTFTDNWDLKTSKRLFRTCISIKEVMLDSDLGLTVFEFGSLLVFKDATVGPLLTRAFSSVMANPRLDPSYCLQASKSVGLCSKVLSQDAVVTTIYSLTNLLFVGYDGFDAKRKTRASHNSTIYSGTPNGYIDDLQLPSPAHSEISSFSLNRSVEFDEHDYRKVCENAITAIIEVCQECNDETIPSLACTILSQKISKIESPIGTLILRGLTSCAPFSQREFINTVRLLNRLSLDALEHRKTTLLSNLQEAQISLARKLKVQYLNHQSDLYMTYLDEMLTAIINKGDVQQLEHHRPHTEIREIGDQIAIYLKPLAEMLPDVTKSEEPLKITSTHTIDLFRNIWFNMVVHGYNITSKNCQDNIKELERIAYNTPPLASELSWDKTETSLDLNTVLRRGSSNHNVKDHRQILGKIFEIHRTMSYPKLMFLAATVFVESLRVRSGDCSTILLYFSDPSVKTSGADKYISQIAFQIVKDYIQLINTGANKQFSSDYIAEQLTNMLVLCSHPIEDLQDCAVQCCDLLINKVSSALCHKRSLFSLFDLLTLLFDSLMDADTHQYEPTTTFIAKCTGIKLLLSDDYQWRSATFKRFHDKSKYWMKLLLYKCNIDVKSLIQSYVSSTQEKEVEFESKIQFGVSFALEMAGSISSHDRELSSISLYNSANLDTLPTIVSQLSWRNHFIAEIINKQPLRTEEETNKAFREIRDRVYDARNTMSEQTNDKIISLLVEIAGLVLFNDSNHGELIRYIVELPFAKFDKKSMNVATAIWFVVMKDKPNLSTLLLSEIAKKWEESIDLSQGMFSQQHDIDNPEFNKMEYAPTNSPRVYKLADQTNASFQPHLEIIKLFRSNFEATLNQSDHLLKVFTRFVEYGLRRMNVASYHPLARLPRFELIRFAFDVLNYHKKLGSRSSCSLTELILDAGLSWFRQRSQYPFGRDALRFESVFSLLKDVARLTDHLKCSSSQVMKQQILLYFLDDEVSKFNVWLNCLNPRDTRGTYIDDPIQNTHITKAYEFDPILAVNLSLRYKIKNLDEQLQKLITDNPLPAIHYPDAVQYFIGINAGTAMPSHHLLFWAKLCPIDAITLFLPPFGSNPYVLQFTMRSLEYHDVNVTFFYVPQIVQSLRFDAKGYVERFILETAKVSQLFAHQIIWNMLANSFKDDESTVPDDLKPTLDKIQREMISSFSATDRVFYEKEFGFFNQVTGISGKLKPYIKKSKAEKKEKIDEEMALIQLEQGVYLPSNPDGVVVDINRKSGKPLQSHAKAPFLATFKIEKEDEDHNIITKWQSAIFKVGDDCRQDVLALQLISMFRTIWSDAGLDLYVFPYRVTATAPGCGVIDVLPNATSRDMLGREAVNGLYEYFITKFGGENSIEFQKARNNLVKSLAAYSVISYLLQFKDRHNGNIMYSEEGHVLHIDFGFCFDIVPGGVKFEVAPFKLTREMINVLGGSDTNTQAFRWFEELCVKGFLACRPYMETIVRTVTPMLESGLPCFKDTTIKNLRSRFVPGKSEMEASAHFRKLVKKAMESFYTKGYDEFQRLTNGIPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJT3</accession>
    <name>G3AJT3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-binding TFAR19-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_59393</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PDCD5 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373568.1">
        <property type="nucleotide sequence ID" value="XM_007373506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJT3"/>
    <dbReference type="FunCoup" id="G3AJT3">
        <property type="interactions" value="423"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJT3"/>
    <dbReference type="GeneID" id="18872896"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59393"/>
    <dbReference type="eggNOG" id="KOG3431">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122978_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJT3"/>
    <dbReference type="OMA" id="MQYEMQK"/>
    <dbReference type="OrthoDB" id="10252486at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.140">
        <property type="entry name" value="PDCD5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002836">
        <property type="entry name" value="PDCD5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036883">
        <property type="entry name" value="PDCD5-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840:SF0">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01984">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015730">
        <property type="entry name" value="TFAR19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46950">
        <property type="entry name" value="Double-stranded DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="19"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="110"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="14936" checksum="2C3C6C6F3D912D7E" modified="2011-11-16" version="1">MDDAELNAIRAARLAELQKNAAGGSGSAPSSSAPTGNAGSSAGTDDVANTILARVLDTHARERLNRVKMVRPDRAQAVEQYILKLYSMGQLQRKLGEDEVVEILDGISRDSSQKAQTKITYNRKHVDDDDDDDDFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJW7</accession>
    <name>G3AJW7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Extracellular membrane protein CFEM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_65206</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373602.1">
        <property type="nucleotide sequence ID" value="XM_007373540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJW7"/>
    <dbReference type="GeneID" id="18875028"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65206"/>
    <dbReference type="HOGENOM" id="CLU_1504358_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJW7"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442182" description="Extracellular membrane protein CFEM domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="16741" checksum="42DD39A4192DED35" modified="2011-11-16" version="1">MKFSTTILATLITLTSATGIHELNAIYVAKVMKRDECSICEKVGPKLEQCFPDYPNYDAVKVATCVCKFDDKFFADYIDCITNCDRFQNNLSSDIDDPKALKQAFCKVAGESSSSAAAAADDSDVAAAVDGQVNTATKNSASTIGVSFIALLGISLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJW9</accession>
    <name>G3AJW9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Potassium transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_59429</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TrkH potassium transport family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373604.1">
        <property type="nucleotide sequence ID" value="XM_007373542.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJW9"/>
    <dbReference type="STRING" id="619300.G3AJW9"/>
    <dbReference type="GeneID" id="18872912"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59429"/>
    <dbReference type="eggNOG" id="KOG1341">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005947_1_1_1"/>
    <dbReference type="InParanoid" id="G3AJW9"/>
    <dbReference type="OMA" id="NDFIDAR"/>
    <dbReference type="OrthoDB" id="9999863at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140107">
        <property type="term" value="F:high-affinity potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004773">
        <property type="entry name" value="K/Na_transp_Trk1/HKT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015958">
        <property type="entry name" value="Trk1_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051143">
        <property type="entry name" value="TrkH_K-transport"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00934">
        <property type="entry name" value="2a38euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064:SF30">
        <property type="entry name" value="HIGH-AFFINITY POTASSIUM TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064">
        <property type="entry name" value="POTASSIUM TRANSPORT PROTEIN DDB_G0292412-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002450">
        <property type="entry name" value="K+_transpter_TRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="46"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="412" mass="46726" checksum="A6D63A3E4A065D40" modified="2011-11-16" version="1">MPDHKSRLEGLAINPAWWAFFTAQSAFNDLGFTLTADSMLSFNQSALVLVVCSFFIVIGNTGFPVFLRFIIWLLFKTAKPLSLYKESLGFLLDHPRRCFTLLFPSAPTWWLFAILVILNGFDLVIFCIVDLKDKPFETIPMGYRVLGGLFQAFCTRTVGFTVMDLSELHAAVQVSYMLMMYISVMPLAISIRRTNVYEEQSLGVYARENNQSDIENGTPSNYVGAHLRNQLSYDIWYIFLGLFVICLAEGGRLRQQDFRFSVFAILFEIISAYGTVGMSLGYPTVNTSLSGQFTVISKLVIIAMMIRGRHRGLPYTIDRAIMLPDAEMRKHDRMQETHALQRSNTLEQSATHTLRRVATQGGSGGPIDAGQNLLGRVYTNVQDYRRRRHERKEAKKLTSRNPNYIVTTVSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK40</accession>
    <name>G3AK40_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_65876</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374366.1">
        <property type="nucleotide sequence ID" value="XM_007374304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK40"/>
    <dbReference type="GeneID" id="18875065"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65876"/>
    <dbReference type="HOGENOM" id="CLU_906631_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK40"/>
    <dbReference type="OMA" id="WCYLTTS"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442189" evidence="2">
        <location>
            <begin position="20"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="307" mass="30170" checksum="65F3995B2E174B83" modified="2011-11-16" version="1">MLISKLILTWCYLTTSALALPISPGGDNKDDDTSVSSTPFGNGNPEDNGHGNEDSASTSPIVVSVESSDVVPTSFPTSSTEIVPTQATSSTEVVLSSVASSSDVPIFTPSEVALSSTEPSADVAASSIVVASSAPTTSSTSESQAVISSEVVPAPVESSSEVTAAPSSEVAPPPADSTSIIIPTSSAAPPIPDTSSSDTIIVVSSSAAPPIVDTSSAAPPIPDTSSAAPPIADTSSSDTIIVVSSSAASPIADTSSAAPSILDTSSAAPLVVATSVADTLSIDTLDTYVAAITSSSTYLAVQPLAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK81</accession>
    <name>G3AK81_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Prolyl 4-hydroxylase alpha subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_60880</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375055.1">
        <property type="nucleotide sequence ID" value="XM_007374993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK81"/>
    <dbReference type="GeneID" id="18873614"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60880"/>
    <dbReference type="eggNOG" id="ENOG502QR14">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041456_0_1_1"/>
    <dbReference type="InParanoid" id="G3AK81"/>
    <dbReference type="OrthoDB" id="69177at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="20242" checksum="6D6145D760F1E262" modified="2011-11-16" version="1" fragment="single">MAPKKNKSSKPVAKNYTFPQGFNDLSNAKSFYIPTPESIVSDQIITINNFFSKELCNELIKSFEQEISLETTPLIKSKEYAARFNDRASLTDLKSADTLWKYLRKILLQEEDEYIDDEGREIREIFANARGLNPQLRVYRYKKGHHFNKHYDESVVCDIPPNGTKRGKTKWTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK94</accession>
    <name>G3AK94_SPAPN</name>
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        <recommendedName>
            <fullName evidence="15">Ergosterol biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_60891</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERG28 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375068.1">
        <property type="nucleotide sequence ID" value="XM_007375006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK94"/>
    <dbReference type="FunCoup" id="G3AK94">
        <property type="interactions" value="277"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK94"/>
    <dbReference type="GeneID" id="18873620"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60891"/>
    <dbReference type="eggNOG" id="KOG3455">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114589_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK94"/>
    <dbReference type="OMA" id="NIAIWTY"/>
    <dbReference type="OrthoDB" id="6485510at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005352">
        <property type="entry name" value="Erg28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15451:SF19">
        <property type="entry name" value="ERGOSTEROL BIOSYNTHETIC PROTEIN 28 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ERGOSTEROL BIOSYNTHETIC PROTEIN 28-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03694">
        <property type="entry name" value="Erg28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="8" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="11" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW33553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="151" mass="17373" checksum="A7E439F5D4587445" modified="2011-11-16" version="1">MIEYFQSLLPYTTPQGGKLPYWLLFISIVSIFNSYQTYQSKDLQLTKRVYENESSPSQPSQPQVTHLSARTFGTWTLITSIVRFYGAYNVTNKAVYELVQFTYLVAGWHFFSEWLIYKTCKLGKGLAGPLIVASVSSIWMYLQKDFYLGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKE9</accession>
    <name>G3AKE9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Protein YOP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_135616</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required to generate and maintain the structure of the tubular endoplasmic reticulum network and the vacuole. Induces high curvature in membranes and causes membrane tubule formation. Involved in membrane/vesicle trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374421.1">
        <property type="nucleotide sequence ID" value="XM_007374359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKE9"/>
    <dbReference type="FunCoup" id="G3AKE9">
        <property type="interactions" value="362"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKE9"/>
    <dbReference type="GeneID" id="18869906"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135616"/>
    <dbReference type="eggNOG" id="KOG1725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028431_2_1_1"/>
    <dbReference type="InParanoid" id="G3AKE9"/>
    <dbReference type="OMA" id="DTQYWVV"/>
    <dbReference type="OrthoDB" id="10009287at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180020">
        <property type="term" value="F:membrane bending activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048309">
        <property type="term" value="P:endoplasmic reticulum inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990809">
        <property type="term" value="P:endoplasmic reticulum tubular network membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032581">
        <property type="term" value="P:ER-dependent peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051292">
        <property type="term" value="P:nuclear pore complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF161">
        <property type="entry name" value="RECEPTOR EXPRESSION-ENHANCING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="176" mass="19659" checksum="ABE8AA2082EF7479" modified="2011-11-16" version="1">MSALPEQLKKYLTQVDDATSALPLLHHFESNTGLPRSYFVIGFTLFYFIMIFVNVGGLGQLLSNIVGLVIPGYYSLLALQTKTTKDDTQLLTYWVVFAFLNVIEFWSSAILYWMPFYFLFKAIFLVYTGVPAFGGANVVYLNVIKPLADPYFAANEDSVAKKIDEAAEAVSSSVHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKR3</accession>
    <name>G3AKR3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_137814</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374482.1">
        <property type="nucleotide sequence ID" value="XM_007374420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKR3"/>
    <dbReference type="STRING" id="619300.G3AKR3"/>
    <dbReference type="GeneID" id="18870079"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137814"/>
    <dbReference type="eggNOG" id="ENOG502S3FG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011909_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKR3"/>
    <dbReference type="OMA" id="WDFSWVL"/>
    <dbReference type="OrthoDB" id="2534600at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052783">
        <property type="entry name" value="Metabolic/Drug-Res_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47655">
        <property type="entry name" value="QUINIC ACID UTILIZATION ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47655:SF2">
        <property type="entry name" value="QUINIC ACID UTILIZATION ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="11"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="793"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="817" mass="91573" checksum="1639DD33A60D0D23" modified="2011-11-16" version="1">MNPKRQRVKQACDHCRSKKAKCDGLMPTCSSCKFNHETCTYAESSKRRGLPPGYTRDLEKKVALLSALIGELCHEDHTVKAKMRELIHDEDRMLRIIGESKWETSLITSLSDQFINKYGGKVPERKQYIKPEPNLSNQLQPPLAEDIPTIQIPAASQQPIPAVPVSVPVPAVPPVQHAMPPVASVQPTSTPFFYDPISDPSFFFNYDIFQFISDEVEPPTSAAWEPIALQYHGLSQLISGFTNRAIQKYNFARNPFRVGSIFNVSSFVNAIKPPPEIFAFPINSQAIVDDYFRIYHPWIPMLDRVSIVRQVSQLTKPRPPIPTDGGQSPPPDLNLIALVWAVLAIGQLAASPLEITNTQTYAKHAIVALENSITSTIETIQAQVLLGMYYYQIGLWDFSWVLISSGSRMAIDVRLMRPSAPGGPENEIEQSANGKKYITTLNNINRERTWASVFIINTVLAARMGRSPVVRAMDWPIPSINTDGWEEWESWEGDRTKIKLDAGRFLSTFNEAIKVVSIVNLAITSNIDTSKGMLDDEIGGVKIEQEQDFTNSNTLTIAKFKSMLNDWYDDLPDYCRIEQYTPDAIPAPIAFLHLGRDLTWCILAIRLSSLKDVDVSINNRILKSRDFQYTRAITSIRGIINERTVAHLQYYPFIDYFLIMCLNFPHMIDLPPHERDKVIRDFRSCLTSASATSIPCKISLDIYKLMNTDIDNSAKESPMIANLLNSPEESVPIGGRSKSLVNGVNNTSTPPPPRLIPSASFPPNTGASPMLPFPPASHMSHLEGLRAFRNDEAGMSTMRQLPRVNSGEYSVKDSHQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL35</accession>
    <name>G3AL35_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_137055</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374594.1">
        <property type="nucleotide sequence ID" value="XM_007374532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL35"/>
    <dbReference type="STRING" id="619300.G3AL35"/>
    <dbReference type="GeneID" id="18870012"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137055"/>
    <dbReference type="eggNOG" id="ENOG502RQCP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171960_0_0_1"/>
    <dbReference type="InParanoid" id="G3AL35"/>
    <dbReference type="OMA" id="SYNIRNR"/>
    <dbReference type="OrthoDB" id="1688044at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="5"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="90" mass="10931" checksum="173AED3B9BD6C4FF" modified="2011-11-16" version="1">MGYTKKEKELLIRHLQAQMQKVDREMEDKIHKQANATRQKLLRRLNGISVTLWDVKIKDVLQLERKKRLVAKTLLRDIQDMKRESFRDMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALB5</accession>
    <name>G3ALB5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_50071</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374674.1">
        <property type="nucleotide sequence ID" value="XM_007374612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALB5"/>
    <dbReference type="FunCoup" id="G3ALB5">
        <property type="interactions" value="259"/>
    </dbReference>
    <dbReference type="GeneID" id="18871399"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50071"/>
    <dbReference type="eggNOG" id="KOG1208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_6_1"/>
    <dbReference type="InParanoid" id="G3ALB5"/>
    <dbReference type="OMA" id="FTWFRYA"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034389">
        <property type="term" value="P:lipid droplet organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006624">
        <property type="term" value="P:vacuolar protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF282">
        <property type="entry name" value="WW DOMAIN-CONTAINING OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW33159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="336" mass="38089" checksum="EB4B0721288BC184" modified="2011-11-16" version="1">MSFDSSEFPFLNPATDRRVAIITGGNSGVGFYTVLQLYLHGYIVYIAGRSKSRCQKSIKELKKKAMAIRSSYSAEQASSRYRGDLHFLEVDLSVLNSVVRAVETFKSMEDHLHILVNNAGAMALPYSVTCDNFEILLQTNYISPFLLTTKLLPMLEKTGDKFPKSGPPRIVYLSSVGHQLAFSYFKLSNTFNYRPNFVFTWLRYGMAKTAGIHFMKMLALRNPKILCMTVHPGLVMNANLFTYFTRLPLVGLLFWCMFQIFGYFFGVTSEEGARAVIKCTLDPQLRAEDDNGKYFGVEGKEAVPSSVARNMDYAARTWIWTIHQLNERGFIIPTND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALE9</accession>
    <name>G3ALE9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein phosphatase 1 regulatory subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_60516</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374707.1">
        <property type="nucleotide sequence ID" value="XM_007374645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALE9"/>
    <dbReference type="FunCoup" id="G3ALE9">
        <property type="interactions" value="344"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALE9"/>
    <dbReference type="GeneID" id="18873446"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60516"/>
    <dbReference type="eggNOG" id="KOG0531">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044236_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALE9"/>
    <dbReference type="OMA" id="EVWASYN"/>
    <dbReference type="OrthoDB" id="266138at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072542">
        <property type="term" value="F:protein phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051457">
        <property type="term" value="P:maintenance of protein location in nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000446">
        <property type="entry name" value="Protein phosphatase 1 regulatory subunit SDS22"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
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        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050836">
        <property type="entry name" value="SDS22/Internalin_LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652">
        <property type="entry name" value="LEUCINE-RICH REPEAT AND IQ DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652:SF3">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="352" mass="40525" checksum="55B7817D71CCCA14" modified="2011-11-16" version="1" fragment="single">MPAEIPDTQRESASGIESNTLVDESVLSHTEDEEEEDDEHEGDDHGENDAVQYPGTVLPDNNVESIEADQDLASTYDIDTDYIDLIHLKIGSIEDLNLSRFTKLQSLCLRQNLITSIVGIKEIADSLTELDLYDNRINHISSSIKHLTKLKNLDFSFNLIKNIKNIETLVELENLYFVQNKIKHIQNLENLTKLKNLELGGNKIEKIENLDNNVNIEQLWLGKNRIYKFENLNHMVNLRVLSIQSNRITKIEGLENLINLEELYLSHNGIEKIENLEHNVNLQVLDVTSNKLKHIENLKHLTKLTDFWCSYNQISSFEEIGKELGKLEELDTVYFEGNPVQTENPSAYRRKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALF6</accession>
    <name>G3ALF6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cx9C motif-containing protein 4, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60529</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CMC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374714.1">
        <property type="nucleotide sequence ID" value="XM_007374652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALF6"/>
    <dbReference type="FunCoup" id="G3ALF6">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALF6"/>
    <dbReference type="GeneID" id="18873451"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60529"/>
    <dbReference type="eggNOG" id="ENOG502S7M4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177210_0_1_1"/>
    <dbReference type="InParanoid" id="G3ALF6"/>
    <dbReference type="OMA" id="YQEEKCQ"/>
    <dbReference type="OrthoDB" id="13601at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1130:FF:000008">
        <property type="entry name" value="Cx9C motif-containing protein 4, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1130">
        <property type="entry name" value="CytochromE C oxidase copper chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027179">
        <property type="entry name" value="CMC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15590">
        <property type="entry name" value="CX9C MOTIF-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15590:SF0">
        <property type="entry name" value="CX9C MOTIF-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08991">
        <property type="entry name" value="CMC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="8"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="40"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR627179-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="84" mass="9685" checksum="5B44F494AE40C4C8" modified="2011-11-16" version="1">MSEVNETCKSNACAIQDCLLRNGYNESKCTKYIDELYLCCKQFYQENGPEASSVCCPKFNLLQLKLKQRSLGQIDAELIEPRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALM9</accession>
    <name>G3ALM9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_151123</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374787.1">
        <property type="nucleotide sequence ID" value="XM_007374725.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3ALM9">
        <property type="interactions" value="1890"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALM9"/>
    <dbReference type="GeneID" id="18870889"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_151123"/>
    <dbReference type="eggNOG" id="KOG0386">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_15_1_1"/>
    <dbReference type="InParanoid" id="G3ALM9"/>
    <dbReference type="OMA" id="NPTRETN"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140008">
        <property type="term" value="F:histone H4 reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17996">
        <property type="entry name" value="DEXHc_SMARCA2_SMARCA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000008">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000843">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014978">
        <property type="entry name" value="Gln-Leu-Gln_QLQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029295">
        <property type="entry name" value="SnAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14619">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00951">
        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01314">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51666">
        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="QLQ" evidence="17">
        <location>
            <begin position="206"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="HSA" evidence="16">
        <location>
            <begin position="589"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="770"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="1083"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="13">
        <location>
            <begin position="1491"/>
            <end position="1561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="51"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="162"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="260"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="702"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1250"/>
            <end position="1273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1366"/>
            <end position="1467"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="502"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="75"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="103"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="162"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="176"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="285"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="368"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="388"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1250"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1262"/>
            <end position="1273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1377"/>
            <end position="1389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1390"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1440"/>
            <end position="1450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW33272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1650" mass="189458" checksum="D43F89BE1DEC1548" modified="2011-11-16" version="1">MNQQMNKEDFQKIIQRWHQLREQFGDQAINVPEFARLNKVINMYKNHQVQRMQQQQQQQQQQQQQQYAKVNSHIPQQQKPQPQPQQLQQQQSHTGTPNMQSYPNQNMGNFNNQQQQPQQQYPPQLQGQSPMAGGVMGQSNNATPLVQNANLMSQARNNMPMQSANQFTGNQMSAGQQQPYPTNQQQPQPLDPGMMQSPNVMGGESAFTTQQFQLLKSQFQALKYLLKSPNSGYPPIPQNLTAFVTNEASAYPNGDYLPAGNPNKIAAPPPKQNINNGIVPPPNQIPIGGQQQQQPGMMSGPSVTPSPNMPPGTPPIEKKKGKRGPKPKNNGQPKKLTKKQLQKVEEERRIEEERQARESIDLQRGIEQSQQQDQPPQQQQQPPPPPSLQQQMSQMSTQPPPKEMKASISPKPSQPVGADPSPPVNIKKLIPDRSKSDKIIIPVNKPNMQVDTFEVPDLVGDYVKDISLSAFYTPQSRLQFPGLLPDGINMDDIVANKEVHLLLSIEKEKDRLRRQLLELSDADEDKKTQLETELAQLELLPYQKELRGKLLIQTWFSKSLLPNSHPNFLARFNTLSLDHVIITEDLYRHQLETLMQAQNQQHKKTIDEILLYSERSSNQVMRRRDKLNRLGNKVASFHNQTAKEEQKRIERMAKQRLQALKSNDEEAYLKLLDHTKDTRITHLLKQTNQFLDSLAQAVQSQQKEAEENLVSSGRIAKPEEPAEPMDDEKREKIEYYNVAHRIKEEITKQPSNLVGGTLKEYQLKGLQWMVSLFNNHLNGILADEMGLGKTIQTISLITYLVEVKKINGPFLVIVPLSTVTNWNLEFEKWAPSVKKITYKGTPNQRKVLQQDIRTGNFQILLTTYEYIIKDKALLSRIRWVHMIIDEGHRMKNASSKLSETLSHSYHSDYRLILTGTPLQNNLPELWALLNFVLPKIFNSVKSFDEWFNTPFANTGGQDKIELSEEETLLVIRRLHKVLRPFLLRRLKKDVEKDLPNKVEKVIKCKMSSLQSKLYQQMLRLNILYAADPADENTAVTIKNANNQIMQLRKICNHPFVYEEVENMINPKAETNDQIWRVAGKFELLDKILPKFKKTGHKVLIFFQMTQIMDIMEDFLRFRNMKYMRLDGGTKADDRTSLLKLFNAPGSDYFCFLLSTRAGGLGLNLQTADTVIIFDTDWNPHQDLQAQDRAHRIGQKNEVKILRLITEDSVEEMILERAHAKLEIDGKVIQAGKFDNKSTAEEQEAMLRALIEKDEERRQKGSDDEEDELDDDEMNEIIARNEGELVTFKQIDEERILTTKNASYKTRLFSDEELPEIYKKDPEELFKRAEEIMEEYGRGARERKTTNYDDHLTEEQWLRQIDGVVSDESDTEVEVKPKRARGRPRGRPRSKGPDAESKDESPEQSQTESEATSKRFLDDSDDFVPVKRQKSATPKLPPVRGRGRGRGRGRGKGSLLNRPTPTVDPLTPDERARLQNVLENILGLILNYKNEHDRRLSDLFLVKPSRKLYPDYYVLIKHPIALDVIKKRIFTKSYTQAREMLEDVHLMFSNARIYNEEGSIVFQDASFLEKLATEKFRELNSDIPEDQLDKILDFTEFDETFNLKPLVVPSAMKHPIDAKLERLDNGESIDSPIIGISTALETEESTPATFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALN4</accession>
    <name>G3ALN4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_60612</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374792.1">
        <property type="nucleotide sequence ID" value="XM_007374730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALN4"/>
    <dbReference type="FunCoup" id="G3ALN4">
        <property type="interactions" value="153"/>
    </dbReference>
    <dbReference type="GeneID" id="18873489"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60612"/>
    <dbReference type="eggNOG" id="ENOG502QVDR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041040_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALN4"/>
    <dbReference type="OMA" id="RCTPDRY"/>
    <dbReference type="OrthoDB" id="4084551at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028000">
        <property type="entry name" value="Pma1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14610">
        <property type="entry name" value="Psg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442449" evidence="3">
        <location>
            <begin position="17"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="384"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="446" mass="51032" checksum="31CF7DD78F1B13C2" modified="2011-11-16" version="1">MKVLSLIAALALCAEAVRLPHVAEETPAPTPIATPARLEKRMRDVIVNKEYAKNQAAKTASSTTELPPKWVRTLSDGKVEIVFPTVIAGVTFSAKPPKTTDGLEPWVSLKDDGSPKTMRPEIKGGRTKNAQPTYGTYFQTATTMYHSKEDLGAHNMADDEVFEEVVYLDEDQTNHKLSPLIRCTPDRYKKKGIARDKSTEPFCTPKDDVRLIKDKTYFVTWYSRFFNKEVKNVKIHLSHIKESLRQKGLKKRDDEAAEEEVKEEVTDEIVLSKRSAVLEHGGKVTELSFYTSDWVSNEQGFFPLYIDEKWFGDEYWRKVLLSIQPDNVADEDFDHKKNSLVVEIWKGAKVSKEHLQDLKKLEEKHAQAYLGDVEIEEGIDYEKYMIMITMPTCVIIAAFGMYLFVTWNRVDLSNVRKRNIRTKITPRCHNTISNLITLSMIRCITC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALU6</accession>
    <name>G3ALU6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein TPS3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TPS3</name>
        <name evidence="3" type="ORF">SPAPADRAFT_70708</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374220.1">
        <property type="nucleotide sequence ID" value="XM_007374158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALU6"/>
    <dbReference type="FunCoup" id="G3ALU6">
        <property type="interactions" value="312"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALU6"/>
    <dbReference type="GeneID" id="18875342"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70708"/>
    <dbReference type="eggNOG" id="KOG1050">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002351_2_0_1"/>
    <dbReference type="InParanoid" id="G3ALU6"/>
    <dbReference type="OMA" id="FEGHYKS"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005946">
        <property type="term" value="C:alpha,alpha-trehalose-phosphate synthase complex (UDP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000099">
        <property type="entry name" value="Alpha,alpha-trehalose phosphate synthase subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF15">
        <property type="entry name" value="TREHALOSE SYNTHASE COMPLEX REGULATORY SUBUNIT TPS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="88"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="206"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="212"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1078" mass="120868" checksum="8BC953D7BD24905C" modified="2011-11-16" version="1">MTVIIGSLFLPYTVKFKIDAATNGSDTESVKETINDFTKSGSPIPSILPALSQTPQIPRSPVLRGRELNRPKISNETRADTPEGFFYARRPRRAGTNESSGEASTSGTPGIVVAPPPSTANAAAAGGGGGSTYIQPNSIVEPKKHFDNSGLPSITTRSQSSTLTRNNSSPNLVSLAPTDNFERARSVLSGRGITLHSNHSASSLFSDEVPFPSTSSTPQHQQQSYVPSYHREDDEPWKEGFKDMIMGRNHSKLAPFGGFSDPSIKHSILNEENIFETAPWNIVPTESGNGSLINAVQLSVQTGVMKRNKWVGILSMPSDDVPENVKENISKELSSNYDCEVVFPDDLTFEGHYKSFCKQILWPTLHYQIPDDPKSKAFEEHSWGHYVLMNRLIADKLVETYKEVNGDADDPYNPENMIWVHDYHLLLVPKMIREKLPNVKIGFFLHVSFPSSEVFRCFAQRKQLLEGMLGANCIGFQTDEYVRHFLQTCNRLLLADTNEYGVSYEGNLTVTNTTPVGIDAASLMHKVTSESVNEWRAMIKEKWKDHKLIVSRDKLDKLRGVKHKLLAYERFLKDNPEYIDSTVLIQICIGESHDADYEAEVMKIVSRINSLPDNISVTQPVVFLQRDIEFDQYLALECEADVFVVSSMREGLNLTCHEFIIATSERKSPLILSEFTGSSALLDCGGKGALLINPWDTKRFSEVFKESLEMSEQEKNLRWGSCFEFVLTRDSKHWVANCLNSILEACNLDRKKSRHLVPFTQKVFENFIRLSGKNGKKIVILGLEISSTSVNSSRNIRTGSAGKVNISEPTRLVGLLVDLIENPNTYVYLMSFLSRHDLDIMFKRYPKIGLIAENGSYVKLIGSKEWITIVDEGEESSWMPQITQLIESKVERLPGSFCQVQDSTVRFHAGRSFIDDRERSLDAMGDTIQHINSLSEELGGIHATLVRNSVIVQKNQINLKALRFLVSYYNTSLYNQSESFIGENIKEFQKPLSPKSETIDFFNRDEHYISAILYSGGLTTIDEANYEYINHLKDIRAVKNALTVTVMATDGEDVKTGAGYEVSGINELLRIFARANLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM39</accession>
    <name>G3AM39_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome c domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_60097</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="12">Binds 1 heme group per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the cytochrome c family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374259.1">
        <property type="nucleotide sequence ID" value="XM_007374197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM39"/>
    <dbReference type="FunCoup" id="G3AM39">
        <property type="interactions" value="596"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM39"/>
    <dbReference type="GeneID" id="18873218"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60097"/>
    <dbReference type="eggNOG" id="KOG3453">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060944_3_0_1"/>
    <dbReference type="InParanoid" id="G3AM39"/>
    <dbReference type="OMA" id="KARCAQC"/>
    <dbReference type="OrthoDB" id="449280at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.760.10:FF:000001">
        <property type="entry name" value="Cytochrome c iso-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="entry name" value="Cytochrome c-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002327">
        <property type="entry name" value="Cyt_c_1A/1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11961">
        <property type="entry name" value="CYTOCHROME C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00604">
        <property type="entry name" value="CYTCHRMECIAB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="Cytochrome c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 10 12" id="KW-0349">Heme</keyword>
    <keyword evidence="9 10 12" id="KW-0408">Iron</keyword>
    <keyword evidence="7 10 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytochrome c" evidence="13">
        <location>
            <begin position="8"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW32744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="110" mass="12011" checksum="90AF38FC4355373A" modified="2011-11-16" version="1">MPAPYEKGSEAKGATLFKTRCLQCHTVEQGGPHKVGPNLHGVFGRQSGQAAGYSYTDANKKKGVTWTEQTMSDYLENPKKYIPGTKMAFGGLKKPKDRNDLVTYLASATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM48</accession>
    <name>G3AM48_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Putative tyrosine-protein phosphatase OCA1</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_60105</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Putative tyrosine-protein phosphatase required for protection against superoxide stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374268.1">
        <property type="nucleotide sequence ID" value="XM_007374206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM48"/>
    <dbReference type="FunCoup" id="G3AM48">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM48"/>
    <dbReference type="GeneID" id="18873221"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60105"/>
    <dbReference type="eggNOG" id="KOG1572">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047845_2_2_1"/>
    <dbReference type="InParanoid" id="G3AM48"/>
    <dbReference type="OMA" id="PWNPISE"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14531">
        <property type="entry name" value="PFA-DSP_Oca1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000035">
        <property type="entry name" value="Tyrosine phosphatase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020428">
        <property type="entry name" value="PFA-DSPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF8">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE OCA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01911">
        <property type="entry name" value="PFDSPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="11">
        <location>
            <begin position="31"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="194" mass="22184" checksum="AB0EBC9C63EACE34" modified="2011-11-16" version="1">MNTTVPNNSLPSNNLRKITKPPAVRIVPPLNFCPVEQQLYRSGQPSIINQAFLNQLNLRTIIWLASEEPSDEFLEYCNSHSINIEFVSMVDDDFDKHMNPWDSLNEPTIKRALELIVNKENYPCLVCCGMGRHRTGTVIGCLRRIQGWNLASVSEEYRRFTGSRGGRILVELLIEGFDISSVHIDKDKVPEWLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMP1</accession>
    <name>G3AMP1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_50360</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC23/SEC24 family. SEC24 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375000.1">
        <property type="nucleotide sequence ID" value="XM_007374938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMP1"/>
    <dbReference type="FunCoup" id="G3AMP1">
        <property type="interactions" value="154"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMP1"/>
    <dbReference type="GeneID" id="18871433"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50360"/>
    <dbReference type="eggNOG" id="KOG1984">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004589_1_0_1"/>
    <dbReference type="InParanoid" id="G3AMP1"/>
    <dbReference type="OMA" id="INPFMTF"/>
    <dbReference type="OrthoDB" id="49016at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050550">
        <property type="entry name" value="SEC23_SEC24_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803">
        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803:SF4">
        <property type="entry name" value="SECRETORY 24CD, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="8">
        <location>
            <begin position="292"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="9">
        <location>
            <begin position="373"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="11">
        <location>
            <begin position="634"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="10">
        <location>
            <begin position="737"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="88"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW33485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1043" mass="115714" checksum="22FD2EA6C1079DE6" modified="2011-11-16" version="1">MSFQPPPSGIFTGNGGYSQPNQRQSEPPLSRHGSTPDLGAQFQAMGLESPRPAFSKKKSKRAVRAYHTEFNSPMGSVNASPVPAHAFPAIPPSTPSSNPYGGFAPMGGGASPGLAQPYTPTAQQFSPAIHQQQQQQQFTPGVQQGHPPPQTTIAADQFHLQDMNNISQSHNLSLQQHRYENQKEYDTPDEQGNPKSFMTFLQTIPPDAGTQFHAVDQGSSSSKFMRSTMYFVPESDSIRQATKLPISVTIRPFAPLLPTEDPVPVVDFSQEDQLAPPAPKKEGELDPLSIGPLRCRRCRAYINPSMQFTANQRFACNICQFPNNHVPEEYSALLDTRGYRADKFIRPELHRSVYDIIVPKEYNFGGAEKKSHPLHVAFLIDITENSIRQSLPLVLADSIRVSLYGLDEPQDENGYSGMGISLEPNTKIALIAYDKRLHFFNLSPQLESAQVTISSDLEDPFVPFSEGLFVDPQESRAVIEDALNYIERLSSFERVADPEPCFAAACRTVAMCLEPYGGGKIISTLCNIPSWGPGGLKFKDNQAVGRAASGTLESEKKMYLADNAYYRSMGKEFVEKNVGLDVHVISHTPVDLSNVGWLANITGGNISRWPNFSMERDARALTAHIVNSYKSTKGYQGQLKVRCSNGLQVSQYYGTSSSIAETNAVLGNVQDPLIPILTKDQTFTVLFEYDGKLSTKLDCHFQAALLYTDATGVRKVRVMNLVLAVTERLEDAFHFCDENAVVTTLVRDTLSFIGKQPMNELRESLNTKLVQVFTQYRAMNEFDHNSARTMSNSLLFPDSLRHLPMYILSLLKTSALRASTGVSADARLADIYQMLNMPVEKLMYHLYPALVELHSLEPHEGIIDQQTGFISLPVYKDLSSKNLDRGVYLLCDGVKVYVWVDPEANIMLLKDLFGQNIESVKEIDPLMDELPHLNTEISNQARTLVNYFQTHIVGLNSLGSAGIQIVRRTIDGAEFAFRELLMEDALGGAIKATSGPSFAEYLTNLHKAIKVQLETDKAAQTVRQSISNVEHKEDTLAQRLLHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMS0</accession>
    <name>G3AMS0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CMP/dCMP-type deaminase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_137171</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375029.1">
        <property type="nucleotide sequence ID" value="XM_007374967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMS0"/>
    <dbReference type="FunCoup" id="G3AMS0">
        <property type="interactions" value="521"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMS0"/>
    <dbReference type="GeneID" id="18870022"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137171"/>
    <dbReference type="eggNOG" id="KOG1018">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081897_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMS0"/>
    <dbReference type="OMA" id="IMCASYL"/>
    <dbReference type="OrthoDB" id="1701769at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052718">
        <property type="term" value="C:tRNA-specific adenosine-34 deaminase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01285">
        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
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        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF149">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="4">
        <location>
            <begin position="6"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW33514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="275" mass="31410" checksum="C2C5F74A0597778C" modified="2011-11-16" version="1">MSSDLSGHFHDMALSMFVAYKALLFNETPVACIVKNTKTGEVTSIGYNYTNTSLNGTQHAEFIAMQRFKDQDVNYKDLVVYVTVEPCIMCASFLRQLGIGKVYYGCGNDRFGGTGTVLSVHDDPNLGEGRYESIGGIMRTEGIQLLRNFYIQENESAPQPKIKKNKEIETKEFPRNEFKFSEDQFIENYGKERIPLYNDGVKELTPVFGKGYSVHDLIDISSVKALPYLEQELGEITQDQLNEFCDLFFNIHDNGEINYDKPIRKYNAKKRHLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN09</accession>
    <name>G3AN09_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Vigilin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_50979</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375699.1">
        <property type="nucleotide sequence ID" value="XM_007375637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN09"/>
    <dbReference type="FunCoup" id="G3AN09">
        <property type="interactions" value="723"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN09"/>
    <dbReference type="GeneID" id="18871504"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50979"/>
    <dbReference type="eggNOG" id="KOG2208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003293_1_0_1"/>
    <dbReference type="InParanoid" id="G3AN09"/>
    <dbReference type="OMA" id="WGPNMKP"/>
    <dbReference type="OrthoDB" id="10027144at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001965">
        <property type="term" value="F:G-protein alpha-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043577">
        <property type="term" value="P:chemotropism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045141">
        <property type="term" value="P:meiotic telomere clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02394">
        <property type="entry name" value="KH-I_Vigilin_rpt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057778">
        <property type="entry name" value="KH_Vigilin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10627:SF31">
        <property type="entry name" value="DODECA-SATELLITE-BINDING PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10627">
        <property type="entry name" value="SCP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24668">
        <property type="entry name" value="KH_Vigilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="39"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="173"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="337"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="566"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="646"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="724"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="807"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="884"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="966"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="1149"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="29"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="55"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1217" mass="134872" checksum="C6335D291B8EE586" modified="2011-11-16" version="1">MPTPAEIIAARLNNENLYDDAVAETLEVPANNEEASAKRSISDEVAFPTLGGKQSSSPSPVPQSIPSWGPSMKTPVSVSSTPKSTRSPTPRVVGNGMKSKVSTIQEAFALNVEDQLNVARPDFIKILTFVKQETKTSIECTTSQHTKKRTFLITGRPEEVKLAKRLVIKKLTKPVQIAFTVPAKLRSRIIGQGGKNLKPIIQANEVKIEIGDEEEEEVEQELADNVAADEDHDDIFSKTVTVTIDGDIEGSKRAKNQILAIVKEETKNLSTKVPVDEIIKPFAQKALESVVTKYADLDFAIPDYKSEKNNIVIVGDRDSVIEARIEIRSILEKLADKITVEEVPIPKVKHQFLPIDQILEEVNVLIQLPKEGESNVKFIGEKSKIKLAQEQARKTTSQYKIEVLDMSKAHRGNLKHVKAVAAHLNQNGVFKEIAQTNDVKINVPEIASLSDETLSSIPIEIVSKGDDEKIKVAKKSIVNHVNRITPDQTQLIQDIDEFLLGKVDETIKDVAKSTGVNYVVLGKNITLFSEKLETEEAEDFEETNAAEDSFKKVDEALNKLRELATKLQTVVLEVDSKDQQHVSGPRGTTLRSILSSVEPNSITVKLHSNGEKQSDNEIFIRGFRESVSAVRKDIEQVISDAQEFKDGYTATIQVPANVISRIIGKSGANLISLRDEFGTKIDVADEKEDNKAKNAKVDVTITGIKRNVEETKSKLASMAKKWADETLVRLRIEHQYHRRMIGPHGIYINRLQDKYNVKIRFPSSDNVPSNFADAPKTKDEVTVKGPSKGVAKAEEELNELYQFEKENGFKQVIQIPTKAVARVIGKAGETIKDIADGSGVEYKFNRNQEEEEKSGFAEVELTGSKSALKEASKKINDIIEEIENFVSTTIKVNPIYHRDLIGQGGSVMKEIISKAGGDDIPRNKHFRLLNIPNEGSGSDEITSQGDKQVVEKIIQQIKDIVALKEASVTEEIDLPKEKHRLIIGPKGTIRHSIQDEFGVTVEIPRSNEESTIVKIVGLPEKIAAAKEKIEELTKDDWNVSIDVPSVYHALVSEHGAIFKQLKSDFNVEVTHGNLTRQASKLSSASTIPTAPEEAYPTDSANYLFTIVENDEELKDDGVVIPWRLKGEEASTAKAAKVIEDRLANAKSAKFTGWFYSAQSSAFSKIIGPQGSKVNQIRKKSNTFITIPRANDKESKFIYLVGSEDNLKIAKEEIQRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN16</accession>
    <name>G3AN16_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_138769</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375706.1">
        <property type="nucleotide sequence ID" value="XM_007375644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN16"/>
    <dbReference type="FunCoup" id="G3AN16">
        <property type="interactions" value="560"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN16"/>
    <dbReference type="GeneID" id="18870149"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138769"/>
    <dbReference type="eggNOG" id="KOG1304">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009646_3_1_1"/>
    <dbReference type="InParanoid" id="G3AN16"/>
    <dbReference type="OMA" id="YWCYYIL"/>
    <dbReference type="OrthoDB" id="1684102at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF666">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="322"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="396"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="434"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="467"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="510"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="556"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="579"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="620"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="248"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="78"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="152"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="646" mass="72172" checksum="476B1FCBA402B326" modified="2011-11-16" version="1">MTEPQNIESSKRRRSIALLTKPPLSQSPYSSSPYSPSPPPHRGSMSVSNSYVANAQFMRGGSGGDDTDSVAMEIDSSKSSSHTNIAIPSNDSLVPPQPELESVDFKAGIDDPNLIKRVSKHLPQDDSLSQEGADITRDLYKISHGQPKLKRSQSYGSMSSRRSSASSFNVPGGFRREFLLQQSAKEESLRTRTPNFLTRNFIEFLSIYGHFAGEDLEDDENIANHYKYLLPSEELPLIDENINPKGTATDKKAYLLLLKAFVGTGVLFLPKAFANGGLLFSILTLVFFAALSYWCYLILVYTKIATKLSSFAEIGLKLYGNWLQRLILFSIVISQIGFVAAYIVFTSQNLRAFISNVSSFNMEDLNMLWFILFQLAIIVPLSLIRDITKLSLSATLANFFIFSGLLTILYFIVYQLFMEGTGENIEYMFNQSEFSLFIGTAIFAFEGIGLIIPIQESMIYPNNFPKVLAQVIATIALIFIVIGTLGYMTFGDQIQTVILLNLPQDSPMIIMTQLLYSFAILLSTPLQLFPAIRLVESKLFFTSGKRSVGVKWLKNLFRTLFVVLTAYIALIGGKNLDKFVSFVGCFACIPLVYMYPPILHLKSCCKIHNGLSEAEKRSRYWLAVLDYILVFIGGVAMIYTTYQILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN24</accession>
    <name>G3AN24_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Nascent polypeptide-associated complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NACB</name>
        <name evidence="15" type="ORF">SPAPADRAFT_61507</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the nascent polypeptide-associated complex (NAC), a dynamic component of the ribosomal exit tunnel, protecting the emerging polypeptides from interaction with other cytoplasmic proteins to ensure appropriate nascent protein targeting. The NAC complex also promotes mitochondrial protein import by enhancing productive ribosome interactions with the outer mitochondrial membrane and blocks the inappropriate interaction of ribosomes translating non-secretory nascent polypeptides with translocation sites in the membrane of the endoplasmic reticulum. EGD1 may act as a transcription factor that exert a negative effect on the expression of several genes that are transcribed by RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Part of the nascent polypeptide-associated complex (NAC), consisting of EGD2 and EGD1. NAC associates with ribosomes via EGD1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the NAC-beta family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375714.1">
        <property type="nucleotide sequence ID" value="XM_007375652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN24"/>
    <dbReference type="FunCoup" id="G3AN24">
        <property type="interactions" value="1219"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN24"/>
    <dbReference type="GeneID" id="18873924"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61507"/>
    <dbReference type="eggNOG" id="KOG2240">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098726_2_2_1"/>
    <dbReference type="InParanoid" id="G3AN24"/>
    <dbReference type="OMA" id="AGDTYME"/>
    <dbReference type="OrthoDB" id="8033832at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005854">
        <property type="term" value="C:nascent polypeptide-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006613">
        <property type="term" value="P:cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22055">
        <property type="entry name" value="NAC_BTF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.30:FF:000001">
        <property type="entry name" value="Transcription factor BTF3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039370">
        <property type="entry name" value="BTF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10351">
        <property type="entry name" value="TRANSCRIPTION FACTOR BTF3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NAC-A/B" evidence="14">
        <location>
            <begin position="33"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="123"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW32438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="158" mass="17213" checksum="539EB59E2B58AFDB" modified="2011-11-16" version="1">MPVDPEKLAKLQKATAKKVGGSRIKAKKNVKTEQDDTKLLEALGKLKATKIEGIEEANFFRDDGKVLHFNRVGVQGAPASNTFAFTGYPQEKNITQLIPQILPQLGAENLEILRQLAEQIQAGKTPRDFNTGAEAAEAAEAEADIPDLVEGQNFDDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN46</accession>
    <name>G3AN46_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein YIP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_55906</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375736.1">
        <property type="nucleotide sequence ID" value="XM_007375674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN46"/>
    <dbReference type="FunCoup" id="G3AN46">
        <property type="interactions" value="329"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN46"/>
    <dbReference type="GeneID" id="18871983"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55906"/>
    <dbReference type="eggNOG" id="KOG3114">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082766_0_0_1"/>
    <dbReference type="InParanoid" id="G3AN46"/>
    <dbReference type="OMA" id="MFQINFY"/>
    <dbReference type="OrthoDB" id="10256463at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039765">
        <property type="entry name" value="Yip5/YIPF1/YIPF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822:SF2">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="26941" checksum="3FE560AD2294FE23" modified="2011-11-16" version="1">MFQINFYRPYFDLDSDMFFYKIQRALNPFATSFGEPNEEENSNQPAELYGFFWITGTLIFLMFVSSTGSNLLAQWLYGDASKKKYEYSFDLLTKSISLFYGYNIIAPGLLYLITTFFYKFPYGLSLSKVISIYGYTNVLWFPITIVNVLIVVLINNQNHHLMLNLFEWIIVVISGIVTGLSNLAKLTPIIKKNSLIIHEGDSSGANRLYYTIVGALAVAHLLFTVLVKISFFGIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANS2</accession>
    <name>G3ANS2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EKC/KEOPS complex subunit CGI121</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">EKC/KEOPS complex subunit cgi121</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_139605</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. CGI121 acts as an allosteric effector that regulates the t(6)A activity of the complex. The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators. CGI121 is not required for tRNA modification.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the CGI121/TPRKB family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375283.1">
        <property type="nucleotide sequence ID" value="XM_007375221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANS2"/>
    <dbReference type="FunCoup" id="G3ANS2">
        <property type="interactions" value="504"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANS2"/>
    <dbReference type="GeneID" id="18870220"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139605"/>
    <dbReference type="eggNOG" id="KOG4066">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065847_1_1_1"/>
    <dbReference type="InParanoid" id="G3ANS2"/>
    <dbReference type="OMA" id="IVCRMST"/>
    <dbReference type="OrthoDB" id="329139at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2380.10">
        <property type="entry name" value="CGI121/TPRKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013926">
        <property type="entry name" value="CGI121/TPRKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036504">
        <property type="entry name" value="CGI121/TPRKB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011465">
        <property type="entry name" value="PRK14886.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15840">
        <property type="entry name" value="CGI-121 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15840:SF10">
        <property type="entry name" value="EKC_KEOPS COMPLEX SUBUNIT TPRKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08617">
        <property type="entry name" value="CGI-121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143870">
        <property type="entry name" value="PF0523-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="180" mass="20524" checksum="8B43E0308F71063A" modified="2011-11-16" version="1">MYITFPQFPKYKVYISLHQNISSEKLQTIKENLASQNSEYNYCFLNAHHIISLAHLQQAIHRSVMNYSRGTMSAKTVNAEIILNLSPVNSIMDAFKNFGIREDCDSAIVVKIIEEDSNEDVSEKLTKLVGPESELNDQVLFDLVDFKRFKKVYKLNDAKFTHTQNDLSKLAIGACILRGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANU2</accession>
    <name>G3ANU2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_55583</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375303.1">
        <property type="nucleotide sequence ID" value="XM_007375241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANU2"/>
    <dbReference type="STRING" id="619300.G3ANU2"/>
    <dbReference type="GeneID" id="18871951"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55583"/>
    <dbReference type="eggNOG" id="ENOG502QR4T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_416171_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANU2"/>
    <dbReference type="OMA" id="INSNYWL"/>
    <dbReference type="OrthoDB" id="3163292at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051089">
        <property type="entry name" value="prtT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845:SF6">
        <property type="entry name" value="TRANSCRIPTION FACTOR SEF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="23"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="641" mass="74728" checksum="4D20CD1C77E5257A" modified="2011-11-16" version="1">MTQKMNQLRLIIPDAKQGKVLKTCVRCRQHKTKCDAITTNPFPCSHCFKKNLSCKLDMVTTKRTTHTNVVNNEVYEKLTNEVEDLKVVLDKLIERRNNMVKLLIQRGAAIQKENMTPSVSQIQSCIQTPEDIPMTPTITASPTSDAFVISTNKSVAPIALTHKQAHILFNNYESNFNKYLPIFPDEFFTSLDLVKFNQENELLFWCIVFTSYLNNPIPNCSDNYKVLAEHIKSLVVEKCWFQTPRSVYVISSLLILTTWPIPNNSGKIGDHLAIKFISTMKSLSYQFGLHKLEFIDEFSHKTKMNISKDINLNNLIRERIYKFININSNYWLISLGLSNNNYNGFTQDYIINKSSNIDIYKDTSPCDHYINSMLKISMIQAKLNENMNVLLNDTNESIMMMPNQINTTKLINFNMFEIILNDLSKMLGKHDYLDNLISVSIEYSKLQLFIYSLSQSDITILEYKKYITKIVNCSFTILTLVKDLQFNQLPAYYKLPIEFSLLILIRIFKSPILNSYSDYQFVKNLINQFYKIIFSDSKWRFINSKLAKIVSKFDKLDNLFIIDKMKDVNGNPSFFLVNKMKNYLCFSLNYELIWLIFERERSDKMDEQSKGNKVINWDTFGISDDNYDFINYIKSNESVFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP52</accession>
    <name>G3AP52_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="14">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_138794</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DEAD box helicase family. DDX55/SPB4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="14"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375359.1">
        <property type="nucleotide sequence ID" value="XM_007375297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP52"/>
    <dbReference type="FunCoup" id="G3AP52">
        <property type="interactions" value="1214"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP52"/>
    <dbReference type="GeneID" id="18870151"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138794"/>
    <dbReference type="eggNOG" id="KOG0345">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_26_4_1"/>
    <dbReference type="InParanoid" id="G3AP52"/>
    <dbReference type="OMA" id="AYKEHEC"/>
    <dbReference type="OrthoDB" id="7396459at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17960">
        <property type="entry name" value="DEADc_DDX55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056330">
        <property type="entry name" value="CTT_SPB4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23681">
        <property type="entry name" value="CTT_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 13 19" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="18">
        <location>
            <begin position="10"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="41"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="269"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="545"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="10"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="554"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="586"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW32083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="617" mass="69569" checksum="B94CDAE0E31C395F" modified="2011-11-16" version="1">MKTKTESLAWENLRYDLHPWLRSAIESLGFATMTPVQASTVPLLSGNKDVVVEAVTGSGKTLAFSIPVLQKVSDRIYGRGGGDNDDDDEDTVEPVKRGHMLAVILSPTRELAAQIQTVIDGVIAYLPEYLKQIKTQLLVGGSSTVRDDLDSFLKEQPQILVGTPGRMLDFLKSNYVKTQSVEIAILDEADKLLSMSFETDVIDILKRLPKQRRTGLFSATISSAGDTIFRTGMNNPVKVVVKSKNITGEQSSAPASLQLAYMMVNPERKLTTLITLLSNYQFKKAIVYFPTCTSVKHFYSMLNYLNCDQLKFHSLHGQLSTKSRLKTLSKFTEGDTNVAKHILMTTDVAARGIDIPDVDLVIQLDPPTDPSMFLHRCGRTGRANKVGRAIVMLNNDSNEEDYIGFMEVKGVTLLQMPVPETPNHASIQNKIRKYMLEDRARHELGVKSYVGFVRYYSKHVASSIFRMAVLDYLGVAKLYGLLRLPKMPESRYVTNMPEDGWLGEPINMDTYAYADQLAEKSRLSTLEADKEKKIQDAKRRKELKVKNEAWSSKMETKESRQERREKVKRKRELIEKQLMEEESGDEKEAVEDWKDIVRKNKKSKSGNGIMQGSFDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>G3APA4</accession>
    <name>G3APA4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GB1/RHD3-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SEY1</name>
        <name evidence="12" type="ORF">SPAPADRAFT_72037</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="8">Enriched in the cortical ER. Concentrated in punctae along the ER tubules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family. RHD3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375951.1">
        <property type="nucleotide sequence ID" value="XM_007375889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APA4"/>
    <dbReference type="FunCoup" id="G3APA4">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APA4"/>
    <dbReference type="GeneID" id="18875409"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72037"/>
    <dbReference type="eggNOG" id="KOG2203">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011270_0_0_1"/>
    <dbReference type="InParanoid" id="G3APA4"/>
    <dbReference type="OMA" id="PIIKMTE"/>
    <dbReference type="OrthoDB" id="1597724at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048309">
        <property type="term" value="P:endoplasmic reticulum inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016320">
        <property type="term" value="P:endoplasmic reticulum membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000727">
        <property type="entry name" value="Protein SEY1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03109">
        <property type="entry name" value="Sey1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008803">
        <property type="entry name" value="RHD3/Sey1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046758">
        <property type="entry name" value="Sey1/RHD3-like_3HB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923:SF2">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05879">
        <property type="entry name" value="RHD3_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20428">
        <property type="entry name" value="Sey1_3HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="1"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="735"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="8">
        <location>
            <begin position="756"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="759"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="780"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="domain" description="GB1/RHD3-type G" evidence="11">
        <location>
            <begin position="90"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="34"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="100"/>
            <end position="107"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="834" mass="95259" checksum="C3EB190EBCDF3B12" modified="2011-11-16" version="1">MPQSATRVSAIFSFPNLETRHMVATDTTKKDISEGPLSKTSSSSSFVPVSPNVASNSGIQDAIQIIDEHKQFNPSILNYVEATASNTSIGNNYHIISVFGSQSTGKSTLLNRLFHTNFDVMDETQRRQQTTKGIWMAHSPRVTITDGEHERPQSDIFVMDVEGTDGREHGEDKDFERKAALFAIATSEILIINIWETQVGLYQGANLGLLKTVFEVNLSLFGKSKLDKKNDNDHRILLLIVIRDYAGITPVENLADTVTEDLKKMWNDLAKPADMTELKFSDFFDIDFHTLNHKLFQPEQFSAGIARLGDRLIVKDDLFKAEYHHNIPIDGWTMYAENCWERIENNKDLDLPMEQILVAKFRCDEIIKEVVDEFKLKFNELFGNKEFTDFEELGKVMSDLYDDSVENFDQSASRYNKSVYEEKKYELCEKLHVYYKQLFEKQSQKLISDTLSKFQKDLISLKGKNFKSKSEELSKDVCELVATSLGYISLNGDIPVNESISSFDAEVDNIVSKQRATELNSIVNKSVKKLSSGISKAVQQELANADDETWDRILAAFKKLSVLPEFDLGTTDEQNKTAQETFKFKSWSQFHDTLSKLISKDKLLIILQDRFDDKFRYDSNGIPKLYFNERDLEDSFQVAKMHAVSIVPILSLAKLSNDSEILPEVDIFDYDLREKYLGESAHDDDDDDEEDEEHTCFAEIMSEGEKAELLAKFKKEIDAKFVENKRAIVQSVTQIPYYIYIIILVLGWNEFLAVIRNPLFFALCLVFGSALYAMYTMNLLKPAMIVAQRMTDEVIVLAKEKLRQVLIDEPPLPSRVATKEEITEEIELDDLSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3APD0</accession>
    <name>G3APD0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Diadenosine and Diphosphoinositol polyphosphate Phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_139414</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375384.1">
        <property type="nucleotide sequence ID" value="XM_007375322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APD0"/>
    <dbReference type="FunCoup" id="G3APD0">
        <property type="interactions" value="236"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APD0"/>
    <dbReference type="GeneID" id="18870207"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139414"/>
    <dbReference type="eggNOG" id="KOG2839">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_5_3_1"/>
    <dbReference type="InParanoid" id="G3APD0"/>
    <dbReference type="OMA" id="EDQWPEM"/>
    <dbReference type="OrthoDB" id="10252405at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034431">
        <property type="term" value="F:bis(5'-adenosyl)-hexaphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034432">
        <property type="term" value="F:bis(5'-adenosyl)-pentaphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008796">
        <property type="term" value="F:bis(5'-nucleosyl)-tetraphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000298">
        <property type="term" value="F:endopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052745">
        <property type="term" value="F:inositol phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052846">
        <property type="term" value="F:inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 1-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052847">
        <property type="term" value="F:inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052843">
        <property type="term" value="F:inositol-1-diphosphate-2,3,4,5,6-pentakisphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052845">
        <property type="term" value="F:inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990174">
        <property type="term" value="F:phosphodiesterase decapping endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901911">
        <property type="term" value="P:adenosine 5'-(hexahydrogen pentaphosphate) catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901909">
        <property type="term" value="P:diadenosine hexaphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901907">
        <property type="term" value="P:diadenosine pentaphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071543">
        <property type="term" value="P:diphosphoinositol polyphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071545">
        <property type="term" value="P:inositol phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006798">
        <property type="term" value="P:polyphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04666">
        <property type="entry name" value="NUDIX_DIPP2_like_Nudt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047198">
        <property type="entry name" value="DDP-like_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12629">
        <property type="entry name" value="DIPHOSPHOINOSITOL POLYPHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12629:SF0">
        <property type="entry name" value="DIPHOSPHOINOSITOL-POLYPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="6">
        <location>
            <begin position="40"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="195" mass="21612" checksum="B7E4CE4C07B9F84F" modified="2011-11-16" version="1">MQSSSESSESLPSSVRRSGKTLPMESRTGRSNQCYNPDTGARIVAGCICLNPERTKVIMISSSAHAGKWVLPKGGHENDETLVDTAMRETWEEAGVEGVVVSELPVILDSRKAAPVIKGDFDPKVAVPKSEFHFFELVVDKMDQEWPESKTRQRRWCTYSEAKHELLKAKRPELVTALNSSSIVKDATEGIDDSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3APJ9</accession>
    <name>G3APJ9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Post-GPI attachment to proteins factor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_138601</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the lipid remodeling steps of GPI-anchor maturation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PGAP3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375446.1">
        <property type="nucleotide sequence ID" value="XM_007375384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APJ9"/>
    <dbReference type="GeneID" id="18870133"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138601"/>
    <dbReference type="eggNOG" id="KOG2970">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032917_1_1_1"/>
    <dbReference type="InParanoid" id="G3APJ9"/>
    <dbReference type="OMA" id="WNIWDIE"/>
    <dbReference type="OrthoDB" id="419770at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007217">
        <property type="entry name" value="Per1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13148">
        <property type="entry name" value="PER1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13148:SF0">
        <property type="entry name" value="POST-GPI ATTACHMENT TO PROTEINS FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04080">
        <property type="entry name" value="Per1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2 7" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5016486932" description="Post-GPI attachment to proteins factor 3" evidence="7">
        <location>
            <begin position="20"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="334" mass="39424" checksum="3D01496E9FB9F2EB" modified="2011-11-16" version="1">MKILLVTLLLLAVGYCSIGDTLPEFQTCLEHCQCTDSSWLWSCESVCNYQCQQLITNQRESIGLEMVQFYGKWPFIKVFGVQEFFSTLFSLGNFYVNYHNLFKLLRQYRRGGSGSEYQVMYSQYVILLVASCIGWIFSTVFHFRDVPLTETLDYFGAFAIVLSNLNAITVRFFQLYKHTIILRIWQASLVMLYIYHTTRLLDFWDYQYNMQMNVFVGLTAMILWIAHSLSKYREFQSHFHIYNNSIQILPYETRLLTKLNYLWISRSSLIPLIPIFNNIFLLGGLSFEMNDFAPIARLVDAHSLWHLTTIFPSIIWHDWNIWDIESLKMVDSMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3APS5</accession>
    <name>G3APS5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">General amino acid permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_61325</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375522.1">
        <property type="nucleotide sequence ID" value="XM_007375460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APS5"/>
    <dbReference type="GeneID" id="18873843"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61325"/>
    <dbReference type="eggNOG" id="KOG1286">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_12_0_1"/>
    <dbReference type="InParanoid" id="G3APS5"/>
    <dbReference type="OMA" id="VSQCGVW"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000017">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF1">
        <property type="entry name" value="GENERAL AMINO-ACID PERMEASE GAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="220"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="346"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="386"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="1"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="458" mass="50678" checksum="2BFD37E9425B83F7" modified="2011-11-16" version="1">MQGLGELSVTFPISGSFNVFASRFIEPAIGFAVGWNYFLQFFVLLPFELIAGAITIKYWNSSINSDVFVIIFWVFVVFITLLGVRWYGEAEFMFSMVKVIAVIGFIILSVVLICGGGPSHDFIGGKYWNHPGPFANGFKGVCSVLVTAAFSYGGTEMVGLTAAETPNPRKFLPKAIKQVFWRIVIFYLLSITMIGTLVAYDDTRLMGSSSVDVTASPFVIAIVHGGIKGLPSVINAVILLAVLSVGNSSVYATSRSLNSLAEQGMAPKWTGYVDRKGRPLVAILVTDVFGLFALIAASDKQEEVFNWLMALSGLSSIFTWLSINISHIRFRAAMKAQNRSISELPYVSQCGVWGSYYGAILNVLVLVAQFWIALFPLNGSPNASDFFQSYLGLPVTIATWLGYKLWKGDWRLFIHAKDIDIDTGRAHVDLDLLQQEVAEEQAALAEKPFFIRWYRFWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3APS9</accession>
    <name>G3APS9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methylthioribulose-1-phosphate dehydratase</fullName>
            <shortName evidence="7">MTRu-1-P dehydratase</shortName>
            <ecNumber evidence="7">4.2.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MDE1</name>
        <name evidence="9" type="ORF">SPAPADRAFT_61327</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>5-(methylsulfanyl)-D-ribulose 1-phosphate = 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:15549"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="ChEBI" id="CHEBI:58828"/>
            <dbReference type="EC" id="4.2.1.109"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 2/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the aldolase class II family. MtnB subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.109" evidence="7"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375526.1">
        <property type="nucleotide sequence ID" value="XM_007375464.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APS9"/>
    <dbReference type="FunCoup" id="G3APS9">
        <property type="interactions" value="254"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APS9"/>
    <dbReference type="GeneID" id="18873844"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61327"/>
    <dbReference type="eggNOG" id="KOG2631">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006033_4_0_1"/>
    <dbReference type="InParanoid" id="G3APS9"/>
    <dbReference type="OMA" id="WFPGTSG"/>
    <dbReference type="OrthoDB" id="191080at2759"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00875"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046570">
        <property type="term" value="F:methylthioribulose 1-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000003">
        <property type="entry name" value="Methylthioribulose-1-phosphate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03116">
        <property type="entry name" value="Salvage_MtnB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017714">
        <property type="entry name" value="MethylthioRu-1-P_deHdtase_MtnB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027514">
        <property type="entry name" value="Salvage_MtnB_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03328">
        <property type="entry name" value="salvage_mtnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10640">
        <property type="entry name" value="METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10640:SF7">
        <property type="entry name" value="METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="8">
        <location>
            <begin position="39"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="263" mass="29732" checksum="15BB99B56B0BE233" modified="2011-11-16" version="1">MSAPCHCKHSDDGASNGNALNVLSPELQQEFKDSNHPANLICELCRLFYDNNWVTGTGGGISIRDVDGENPNLVYIAPSGVQKERIQPWEMFLVELPDEKILRTPNDIPKELTKSYKYKPSACTPLFMSCYTMRDAGACIHTHSQHAVMTTLLYENEKEFTISHIEQIKALPKLQYNEQTKKVEKLVIPIIENTPHEEDLTDSLQEAIKNYPGTSAVLVRRHGIYVWGETVWKAKVYNEALDYLLELAVKMKLAGIPLVKEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3APW7</accession>
    <name>G3APW7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 13">Protoporphyrinogen oxidase</fullName>
            <ecNumber evidence="4 13">1.3.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_55778</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 13">Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>protoporphyrinogen IX + 3 O2 = protoporphyrin IX + 3 H2O2</text>
            <dbReference type="Rhea" id="RHEA:25576"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:57307"/>
            <dbReference type="EC" id="1.3.3.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="13">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the protoporphyrinogen/coproporphyrinogen oxidase family. Protoporphyrinogen oxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.3.4" evidence="4 13"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375564.1">
        <property type="nucleotide sequence ID" value="XM_007375502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APW7"/>
    <dbReference type="FunCoup" id="G3APW7">
        <property type="interactions" value="511"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APW7"/>
    <dbReference type="GeneID" id="18871971"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55778"/>
    <dbReference type="eggNOG" id="KOG1276">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009629_1_2_1"/>
    <dbReference type="InParanoid" id="G3APW7"/>
    <dbReference type="OMA" id="WFDQWFG"/>
    <dbReference type="OrthoDB" id="438553at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00324"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004729">
        <property type="term" value="F:oxygen-dependent protoporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000276">
        <property type="entry name" value="Protoporphyrinogen oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004572">
        <property type="entry name" value="Protoporphyrinogen_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050464">
        <property type="entry name" value="Zeta_carotene_desat/Oxidored"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00562">
        <property type="entry name" value="proto_IX_ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923:SF3">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0274">FAD</keyword>
    <keyword evidence="5 13" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9 13" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10 13" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="14">
        <location>
            <begin position="18"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW32288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="527" mass="58505" checksum="A613811A1C841F85" modified="2011-11-16" version="1">MSSLLPANSKVAVLGAGISGLSFTYFLSKLRPDLQFHVFEKSSRPAGWINSSYLHVDSSGQDIVLEKGPRTLRGIKDGTLIILDILRELGLSDEIQVVHANSPANKKYIMNQAGEVVQVPNSISTLFKFLTKVNVVDPSLVWGILKEPFVKPNLEDETVEQFIKRRFGSDVLTDNVVSAIFHGIYAGDVGKLSVSTVLPQLKDMERESGSIIRHILKLMRAKKKETGLSSNLQTYESLISPNANFVGLKTQAKNCPMVSLQHGLEVLPHQLTEYLAKRDNITFHYNTPITELDPVKGTVNGEKFNHIRSTINVNSVAKALPSTNSLVPHLKSVNYGTVFLCNIYTKGSNKLIPADKSGFGFLVPRFRNVHSNPQALLGVIFDSEVEKNAVGLFNKIDKKELDYDKITIMMGGHYYSQWGMPSQSINIKIVKQVLEAQLKVDLSKFNIRVVDGDTVEDVKDNDLIISCNYHRDAIPQYEVGYVETHREVDQIVKDGQYLLSFGGTVFGDGLGVPDCVINAFKDALKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3APZ3</accession>
    <name>G3APZ3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61390</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375590.1">
        <property type="nucleotide sequence ID" value="XM_007375528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APZ3"/>
    <dbReference type="FunCoup" id="G3APZ3">
        <property type="interactions" value="1376"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APZ3"/>
    <dbReference type="GeneID" id="18873871"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61390"/>
    <dbReference type="eggNOG" id="KOG1889">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003016_7_4_1"/>
    <dbReference type="InParanoid" id="G3APZ3"/>
    <dbReference type="OMA" id="ITKAQPV"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072517">
        <property type="term" value="C:host cell viral assembly compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017059">
        <property type="term" value="C:serine palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052629">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043812">
        <property type="term" value="F:phosphatidylinositol-4-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061909">
        <property type="term" value="P:autophagosome-lysosome fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662">
        <property type="entry name" value="PHOSPHATIDYLINOSITIDE PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662:SF2">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3-PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="515"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="547"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="SAC" evidence="2">
        <location>
            <begin position="115"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="609" mass="69716" checksum="568AFDBDE5CDFBD1" modified="2011-11-16" version="1">MTFTHSVSADGSHIFQNLNTSNYLLLTSDGSIEVSQTIPSIYDSKLSTGNHSISCIIGVIKLKISSYVIISDQHTITGSILGNEIAKVESYKILPLGNNQFAKSNAEEAEYLKLLNQHLSNATLYFSIDNKYDVTNSLQRQYTTANLQPDPRFMWNKFLSTSLIENGANEFVTPLIYGYFKSHSATFNGPHLLDFALLTRRANSRAGTRYFRRGIDTNGNVANFNETEQIFTAADNHVYSFLQTRGSVPVYWGEINNLKYKPNLVISSKPALLATEKHFTEQVELYGDNYLVNLVNQKGYELPVKQSYEAAVDNLPEHLAKHINYIYFDFHHECKGMRYDRINLLLERLIQLGYTSDNYFSYDLAENKILSVQNKIVRTNCMDCLDRTNVVQGELARWVLQNQFIKSGYIEKDTLVTWNKIDPKFNLFFQNFWADNADAVSCAYSGTGALKTDFTRLGKRTYKGALNDLSNSITRYYKNNLKDGSRQDSYDLFLGNYKPFQDSINSPFIDRRPDYIQLLPYLMGTSLLVLLTIIYYPRGSIFDFKNLVIIGACLYLNARGLFYMLKDGYQFVDWPKLSSLDFLEKEEIGNGSIKYRETEHFKSVGKKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQI4</accession>
    <name>G3AQI4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial matrix iron-sulfur protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_141017</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adrenodoxin/putidaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376309.1">
        <property type="nucleotide sequence ID" value="XM_007376247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQI4"/>
    <dbReference type="FunCoup" id="G3AQI4">
        <property type="interactions" value="449"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQI4"/>
    <dbReference type="GeneID" id="18870338"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141017"/>
    <dbReference type="eggNOG" id="KOG3309">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082632_5_0_1"/>
    <dbReference type="InParanoid" id="G3AQI4"/>
    <dbReference type="OMA" id="TPMEEDM"/>
    <dbReference type="OrthoDB" id="268593at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016653">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140647">
        <property type="term" value="P:P450-containing electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000013">
        <property type="entry name" value="Adrenodoxin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001055">
        <property type="entry name" value="Adrenodoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018298">
        <property type="entry name" value="Adrenodoxin_Fe-S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426:SF65">
        <property type="entry name" value="FERREDOXIN-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="FERREDOXIN/ADRENODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00355">
        <property type="entry name" value="ADRENODOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00814">
        <property type="entry name" value="ADX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="11">
        <location>
            <begin position="35"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="146" mass="16210" checksum="FA0FBBE0EAADEBCD" modified="2011-11-16" version="1">MTNKPIITKTFTAKFHNTSIRSHGHIHKPNPGEELHITFITKDGEQFTYEVAEGDNILDIAQAHNLDMEGACGGSCACSTCHVIVDPDYYDSIPEPDDDENDMLDLAFGLTETSRLGCQVKMTKELDGIRVALPAMTRNLQIKDMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQM5</accession>
    <name>G3AQM5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_62187</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 32 different proteins and 1 molecule of RNA (18S). The 60S subunit contains 45 different proteins and 3 molecules of RNA (25S, 5.8S and 5S).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376350.1">
        <property type="nucleotide sequence ID" value="XM_007376288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQM5"/>
    <dbReference type="FunCoup" id="G3AQM5">
        <property type="interactions" value="1198"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQM5"/>
    <dbReference type="GeneID" id="18874208"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62187"/>
    <dbReference type="eggNOG" id="KOG3166">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055193_0_1_1"/>
    <dbReference type="InParanoid" id="G3AQM5"/>
    <dbReference type="OMA" id="AICVQNV"/>
    <dbReference type="OrthoDB" id="29563at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001921">
        <property type="entry name" value="Ribosomal_eL8_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00882">
        <property type="entry name" value="RIBOSOMALL7A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="8">
        <location>
            <begin position="124"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00063735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="261" mass="28221" checksum="222E919BA3C18133" modified="2011-11-16" version="1">MAPKGKKVAPAPLAAKTSKTASAKNPLIDSTPKNFGIGQSIQPTRNLSRFVRWPEYVRLQRQKKILSLRLKVPPSIAQFSQTLDKNTAAQAFKLFNKYRPETAAEKKERLTKEAAAVAEGKSAKDASPKPVVVKYGLNHVVSLIENKKAKLVLIANDVDPIELVVFLPALCKKMGVPYAIVKGKARLGTLVHKKTSAVAALTEVTAADEAELSKLVSTINANFIEKYEDNRRHWGGGIMGSKANDKIAKRAKAAASAATAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQR6</accession>
    <name>G3AQR6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TEA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_140865</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376391.1">
        <property type="nucleotide sequence ID" value="XM_007376329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQR6"/>
    <dbReference type="STRING" id="619300.G3AQR6"/>
    <dbReference type="GeneID" id="18870324"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140865"/>
    <dbReference type="eggNOG" id="KOG3841">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022080_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQR6"/>
    <dbReference type="OMA" id="VHESSKM"/>
    <dbReference type="OrthoDB" id="10006572at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.40">
        <property type="entry name" value="TEA/ATTS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000818">
        <property type="entry name" value="TEA/ATTS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038096">
        <property type="entry name" value="TEA/ATTS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050937">
        <property type="entry name" value="TEC1_TEAD_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834:SF0">
        <property type="entry name" value="PROTEIN SCALLOPED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834">
        <property type="entry name" value="TRANSCRIPTIONAL ENHANCER FACTOR TEF RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01285">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00065">
        <property type="entry name" value="TEADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00426">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00554">
        <property type="entry name" value="TEA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51088">
        <property type="entry name" value="TEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TEA" evidence="8">
        <location>
            <begin position="139"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="TEA" evidence="6">
        <location>
            <begin position="139"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="500" mass="56542" checksum="8E4B516DBB98FF50" modified="2011-11-16" version="1" fragment="single">MSQVTPTSTPIRSANQKKLPLIVDVAIDNNTGQQLYQVHESSKLVRKEMPKSNHFSRTVIAEETAFFDSNNTNNSNTEKTPTRNVLGTLSPSSLKERKYVDVANYPEPIPTTKRFNGVEFHQDMLAGVPAGLSGNTDVSSSDSDIWSEDVEQAFEEVLKLIPKNGLTKIKIAGRSCGRNELISDYIFTKTGKFRTRKQISSHIQVIKNLGENKNVINLINEGPVYANEEERIDATKKFEEIFSKINLNKSLGFSDSLKRNGNLPQLQAPKKRKSQKLIPKYKFENFFMAVTDQYNLNPIVFSIQHNVIEVKRLKVKSNANISQRFPGMDEFRTCANTPIIHNMVKVVFPPLPKTHSIESGFNSSFTLKREIPNEGFDSTSYPQTFSSFTSIYLFGKESLKFNTDGLTFNDPHDFLVKFWKVFLRNLMDKKDAAINDTLKGLTIKQIMYERSPNSVKQVQDVSMVAKSKIVSVLLWEFARVSDFSEAVTTTTNLVLPPLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQU5</accession>
    <name>G3AQU5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamate pyruvate transaminase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutamic--alanine transaminase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Glutamic--pyruvic transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_140897</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376421.1">
        <property type="nucleotide sequence ID" value="XM_007376359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQU5"/>
    <dbReference type="FunCoup" id="G3AQU5">
        <property type="interactions" value="503"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQU5"/>
    <dbReference type="GeneID" id="18870328"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140897"/>
    <dbReference type="eggNOG" id="KOG0258">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014254_3_0_1"/>
    <dbReference type="InParanoid" id="G3AQU5"/>
    <dbReference type="OMA" id="FGFECPP"/>
    <dbReference type="OrthoDB" id="1732682at2759"/>
    <dbReference type="UniPathway" id="UPA00528">
        <property type="reaction ID" value="UER00586"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042853">
        <property type="term" value="P:L-alanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000140">
        <property type="entry name" value="alanine aminotransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1970:FF:000001">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000012">
        <property type="entry name" value="alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045088">
        <property type="entry name" value="ALAT1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751">
        <property type="entry name" value="ALANINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751:SF29">
        <property type="entry name" value="ALANINE TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="10">
        <location>
            <begin position="104"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00078532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00080525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW31643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="498" mass="55856" checksum="5BEF7A0A9C9DBC33" modified="2011-11-16" version="1">MSPSSTQISRALSTFKPADPLTIHDINPNTVTAKYAVRGKIPIIADGLQDLIKSQPQSHGLPFNKIINANIGNPQQLDQRPLTWYRQVLSILQYPGILETNVDYPKDVVERAKTILESIGSLGAYSHSQGAAYFRKSIADFIERRDGYPSNPHNLFLTSGASTAVSYLIQILSADPNSGFLIPIPQYPLYTATIALNNAKPIGYYLDESNHWSTNPEEIRELIKKNQDEGINIKALVVINPGNPTGSILSPQDMIEIIDIAAEYGLVLIADEVYQENIFKGKFISFKRVLCELLEMEPETYQHVQLASLHSTSKGVSGECGQRGGYMELVGFNTEVRDIVFKLASINLCSVVSGQALMELMINPPKAGDESYELYKAETKAIHNDLEKRADLLYHAFLEMENIECNKPMGAMYLFPSLTFDEEKYHKLYSRAKNSDLGIDDIYCVELLENTGICCIPGNGFGQKPGTYHLRTTFLPPGQEWIDRWTKFHKQFIEKYKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR16</accession>
    <name>G3AR16_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62288</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376455.1">
        <property type="nucleotide sequence ID" value="XM_007376393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR16"/>
    <dbReference type="GeneID" id="18874248"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62288"/>
    <dbReference type="HOGENOM" id="CLU_2612683_0_0_1"/>
    <dbReference type="InParanoid" id="G3AR16"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="79" mass="9680" checksum="A281F6B83BBCDCD7" modified="2011-11-16" version="1" fragment="single">MNDPFLDFNQECFQINQLQFNLPIQQLESCPYCFEIFDNCKTMKDHCIQIHETDYMYICIYCQKPSKRKSDYNRHIRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR20</accession>
    <name>G3AR20_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bacterial surface antigen (D15) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_62290</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAM50/omp85 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376459.1">
        <property type="nucleotide sequence ID" value="XM_007376397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR20"/>
    <dbReference type="FunCoup" id="G3AR20">
        <property type="interactions" value="151"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR20"/>
    <dbReference type="GeneID" id="18874249"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62290"/>
    <dbReference type="eggNOG" id="KOG2602">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014798_3_1_1"/>
    <dbReference type="InParanoid" id="G3AR20"/>
    <dbReference type="OMA" id="SGIWRQI"/>
    <dbReference type="OrthoDB" id="1724197at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.50">
        <property type="entry name" value="membrane protein fhac: a member of the omp85/tpsb transporter family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815:SF18">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY COMPONENT 50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY SAMM50 PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="Bacterial surface antigen (D15)" evidence="7">
        <location>
            <begin position="172"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="486" mass="55047" checksum="D09D12E267956BAD" modified="2011-11-16" version="1">MSQDYNDEFIDNIKRKDSIKNLTDAEKELEKLKNDKQSFMIEQNKQYLESLFQDHKSSPIKIRNVQITNAQTFRDSFLQSQFKSLLASDQSVSLESFLTKVEHISKNFLKLGIIDNLLVSVQQVNPPMFSRGTSLHVVPVFNIIPTKKFYAKTGTNIGNGEGDGYIQFQLRNIFGGGENLEFDAVSGTKTQSSYMLNYNQPLFNNANYRLENLFYVNTRKFDWIQANVTGRGVTNKIHTLFDGPVNHEVVLENYWKVLNNYGSKSLEVLQQAGSQYKSSLSYNIIYDSRNNKHLPTVGKYLQFGVEFNGWAKYVKYPFIKTAGQAQFSYSLPKFKSSVIATAKTGMLYPLGTTSSLLDRFYIGGPNDVRSFMLNGLGPKDFNSSIGGDIFLNGGISLITDIPRYTESNFKIHNFVNFGKLVKMDKSQGIIDNLKQLTHSHSLSYGIGLLFNHPMARFELNLVVPIIAHDRDSVRKGIQYGIGVSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR47</accession>
    <name>G3AR47_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NEDD8-conjugating enzyme UBC12</fullName>
            <ecNumber evidence="7">2.3.2.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">NEDD8-conjugating enzyme Ubc12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">RUB1-conjugating enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Ubiquitin carrier protein 12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_61798</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine + [E2 NEDD8-conjugating enzyme]-L-cysteine = [E1 NEDD8-activating enzyme]-L-cysteine + [E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.34"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.34" evidence="7"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376001.1">
        <property type="nucleotide sequence ID" value="XM_007375939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR47"/>
    <dbReference type="FunCoup" id="G3AR47">
        <property type="interactions" value="822"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR47"/>
    <dbReference type="GeneID" id="18874054"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61798"/>
    <dbReference type="eggNOG" id="KOG0420">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_6_0_1"/>
    <dbReference type="InParanoid" id="G3AR47"/>
    <dbReference type="OMA" id="CQVDFPD"/>
    <dbReference type="OrthoDB" id="10264588at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061654">
        <property type="term" value="F:NEDD8 conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23794">
        <property type="entry name" value="UBCc_UBE2F_UBE2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000005">
        <property type="entry name" value="NEDD8-conjugating enzyme Ubc12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="15">
        <location>
            <begin position="32"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="12">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW31223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="187" mass="21373" checksum="89A92407F35ADAC5" modified="2011-11-16" version="1">MLKLRQLKEKQKAQAAAEGHNNSSTPTTSISAAQLRLQKDITELDLPHTIKVQFPDPSNFFTFNLTITPQQGYYRSGHFHFKVDINNNFPIDPPKIKCLNKIYHPNIDLEGNICLNILREDWSPVLSLSQVLMGLNFLLLEPNANDPLNKQAANVLAKDPRQFERNVYNSMRGGYIDSVYFDNVMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARM3</accession>
    <name>G3ARM3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62370</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376554.1">
        <property type="nucleotide sequence ID" value="XM_007376492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARM3"/>
    <dbReference type="GeneID" id="18874293"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62370"/>
    <dbReference type="eggNOG" id="ENOG502T67D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079441_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARM3"/>
    <dbReference type="OMA" id="CVYRNNG"/>
    <dbReference type="OrthoDB" id="4009574at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057874">
        <property type="entry name" value="Crypto_dsRNA_S"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25640">
        <property type="entry name" value="Crypto_dsRNA_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="305" mass="34775" checksum="22E0E909478DCA3E" modified="2011-11-16" version="1">MVQHKPNIVFFPVDFTEFDNLIKKSLPDDSKEVIRYLHSCFTVYVIVNQQTWRSAGGRPDPFLPCFEKILPRCILPTYFKDIIREMARPMKTVNAIYIPDIPSALADSSQELIMGIVNQYPNLTTVFKKLGLPRLEIEEIEPACFTMYHEQENLISVPRDGFVPAYRLKVVSVLKHIRFQQYRLVTSEASWVSLDRGNGNVVKVSNAIDIFQNTNLTCGFCVFQHHGGVRINSNNLMCASVVGYTLPTHISTERFPTEPRFLKTDAENNHRVAKPRRKSRNKGKIVPILAKPGVLVNLKKSVKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARM8</accession>
    <name>G3ARM8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_155409</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. DDX46/PRP5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376559.1">
        <property type="nucleotide sequence ID" value="XM_007376497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARM8"/>
    <dbReference type="FunCoup" id="G3ARM8">
        <property type="interactions" value="1060"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARM8"/>
    <dbReference type="GeneID" id="18871019"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_155409"/>
    <dbReference type="eggNOG" id="KOG0334">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_0_2_1"/>
    <dbReference type="InParanoid" id="G3ARM8"/>
    <dbReference type="OMA" id="FAQYVHT"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056149">
        <property type="entry name" value="PRP5/DDX46/KHDC4_KH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23469">
        <property type="entry name" value="KH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="15">
        <location>
            <begin position="258"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="290"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="498"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="65"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="11">
        <location>
            <begin position="258"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="92"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW31781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="839" mass="94515" checksum="C1F92D0D903B35FB" modified="2011-11-16" version="1">MNGHTSQGITNQTSIQNENGESKEEKLRKRREQLLAWRQKKEQEKEQEKIDADEKKRIRQQRIEEWKRARAQQSQEPVKKPDVAKPISARPTLTLKKTNKTTAPLKRPRVVLQQDDQESDSAKPKFKKPTLDQQETPANDDAPEVDELDLYLASLSETKNTTKVQLDEPDDLDEEEEVDEEQLKQQKLLTSLTKLQTKGKELKPIDHTLETYAPFRKSFYQQPYELQMLTQEQISEIRKELGNVRVKGNNASHYAPISKWSHLGLPSNLSTVITDKLQFESPSAIQCQALPIIMSGRDVIGVAKTGSGKTLSYVIPMLRHIQDQPPIRENDGPIGVVLCPTRELALQIQRKISNFTSKSLRVCCCYGGSSIEPQINELKSGVEIIVGTPGRVIDLLAANSGRVTNLQRTTYIVLDEADRMFDLGFEPQISKIFTQIRPDRQTILFSATFPRKMEQLAKHILVDPVEIIVGGISVVAPEITQKIILFENVTAEEFKSDRIDKLHSILADYQTYKKVLIFVEKQNDADDLVTQLLAFNLPCVAIHGGKDQLDRRYAIKEFSSINSGVDILIATSVAARGLDVKSLGLVINFDPPNHMEDYVHRVGRTGRAGSKGTAITFVWNKQEHEIANLVRALRMSKVEEIDSRLVEIAESFSSKVKQGKEKIHYGFGGKGLDKLKQVRDSKLQMEKNMYDESTTETVKEKSVTPNATPNATAAALPDFEIIEGNSPETSGPDKCKFYSRIIINDLPQAARWNIVNREHLSKIIETSRTSITTRGQFYPPGKNPPPNNKDSLAKLYLLVEGLDKSSVHEANNMIRERMIEGLELAAQQETNVPGGRYVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARP7</accession>
    <name>G3ARP7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_140928</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HesB/IscA family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376578.1">
        <property type="nucleotide sequence ID" value="XM_007376516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARP7"/>
    <dbReference type="FunCoup" id="G3ARP7">
        <property type="interactions" value="295"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARP7"/>
    <dbReference type="GeneID" id="18870332"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140928"/>
    <dbReference type="eggNOG" id="KOG1119">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069054_1_0_1"/>
    <dbReference type="InParanoid" id="G3ARP7"/>
    <dbReference type="OMA" id="CHGFQYT"/>
    <dbReference type="OrthoDB" id="1938621at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.300.12:FF:000011">
        <property type="entry name" value="Iron-sulfur assembly protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="ATAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="ATAP_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00049">
        <property type="entry name" value="iron-sulfur cluster assembly accessory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43011">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 2 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43011:SF1">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 2 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core" evidence="2">
        <location>
            <begin position="74"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="203" mass="22817" checksum="1F5A1A42C5ECE0B4" modified="2011-11-16" version="1">MIPRLAISRISLRSTLVVRPITLQFARFNSVKTLKPSSFSLPKSDPTLAQSQLIKPEQTDEFSLTKLVHGSTDMSIGITERARDKLNEIASSEAEPDKSVLRIKVESGGCHGFQYNLELSNLDKEMKDDEDDELFVFEREGGKIVLDDSSLTILQDSKLDYTHELIGSQFKIVESPYTSTACGCGSSFDFDFEKLEQKQKEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARQ4</accession>
    <name>G3ARQ4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_62400</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376585.1">
        <property type="nucleotide sequence ID" value="XM_007376523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARQ4"/>
    <dbReference type="FunCoup" id="G3ARQ4">
        <property type="interactions" value="441"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARQ4"/>
    <dbReference type="GeneID" id="18874311"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62400"/>
    <dbReference type="eggNOG" id="KOG1315">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027721_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARQ4"/>
    <dbReference type="OMA" id="YTYFKVI"/>
    <dbReference type="OrthoDB" id="302728at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="141"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="12">
        <location>
            <begin position="56"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="281" mass="33196" checksum="D318E556DADBF459" modified="2011-11-16" version="1">MIGPGSPLDYPELKFNQPLHENPYTENPSSQQQPQQQPPPKHIMTVHTAQINGNQGYHYCDKCQCWKPDRTHHCSSSGRCILKMDHYCPWFSTCIGFFNHKFFVQFLIYVAIYSTFVFVTSFLVVYGFLWGERFHHEFLSLNLVILCVLSFAFSVAVSMFAAFSVYLIFKNLTTIEFQSQRWGSSDRGYNYEFTNKVQNSNIYDLGAWENWKSVMGPDWKTWILPININIKSIHAFDKNGINFKVNQEIYSKWCENAELQHQLNQQLQDYKHRLRRERQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARZ6</accession>
    <name>G3ARZ6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="3 11">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 11">Lysyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_62457</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>tRNA(Lys) + L-lysine + ATP = L-lysyl-tRNA(Lys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="3 11"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376623.1">
        <property type="nucleotide sequence ID" value="XM_007376561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARZ6"/>
    <dbReference type="FunCoup" id="G3ARZ6">
        <property type="interactions" value="1240"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARZ6"/>
    <dbReference type="GeneID" id="18874326"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62457"/>
    <dbReference type="eggNOG" id="KOG1885">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008255_6_0_1"/>
    <dbReference type="InParanoid" id="G3ARZ6"/>
    <dbReference type="OMA" id="DFRNEGM"/>
    <dbReference type="OrthoDB" id="21243at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00775">
        <property type="entry name" value="LysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04322">
        <property type="entry name" value="LysRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000050">
        <property type="entry name" value="Lysine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000044">
        <property type="entry name" value="Lysine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00252">
        <property type="entry name" value="Lys_tRNA_synth_class2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002313">
        <property type="entry name" value="Lys-tRNA-ligase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034762">
        <property type="entry name" value="Lys-tRNA-ligase_II_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044136">
        <property type="entry name" value="Lys-tRNA-ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018149">
        <property type="entry name" value="Lys-tRNA-synth_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00499">
        <property type="entry name" value="lysS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001756">
        <property type="entry name" value="PRK00484.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918:SF9">
        <property type="entry name" value="LYSINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918">
        <property type="entry name" value="LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039101">
        <property type="entry name" value="LysRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00982">
        <property type="entry name" value="TRNASYNTHLYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="216"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="31"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW31845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="595" mass="67806" checksum="0140FABBFF19C762" modified="2011-11-16" version="1">MSDEVNQVTENVNNLHLDKVTGEYVSKTELKKREKLRQRDAKKAEKAAKAAATAPKVAKKKGDEDLTPNQYFEIRSRQINELREANNADPTKFNPYPHKFQVTTKIPEFVEKYSHLQRGETLQDVSVSVSGRIMVKREAGSKLKFYSLKGDGVQVQIMAQAQDAESVEAFEKMHELLRRGDIIGVVGYPGKTAPAKGGDGELSVFAKTVQLLTPCLHMLPTEHFGFKDQEARYRKRYLDLIMNDSTRDRFKIRSKIISYIRKFFDERDFIEVETPILNVVAGGATAKPFVTHHNDLNMEMFMRIAPELFLKELVVGGMDRVYEIGRQFRNEGIDMTHNPEFTTCEFYQAYADVYDIMDMTELLFSEMVKEITGSYVIKYHPNGPEGEELTLNFSRPWKRINMIEELERIYNVKFPAGETLHTEAAGDFMKKLLKDNNIECTPPLTNARMLDKLVGDLEDSCINPTFIFGHPQMMSPLAKKDRNVPGLCERFEVFVATKEICNAYTELNDPFDQRARFEEQARQKAQGDDEAQMVDETFCNALEYGLPPTGGWGCGIDRIAMFLTNSNTIREVLLFPTLKPDALVLKGEEAFADLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS29</accession>
    <name>G3AS29_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Protein transport protein SEC61 subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_62485</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Appears to play a crucial role in the insertion of secretory and membrane polypeptides into the ER. It is required for assembly of membrane and secretory proteins and is essential for cell growth. It interacts with other membrane proteins required for protein translocation. Upon binding to SEC62/63 complex, secretory precursor polypeptides may engage SEC61 to begin membrane penetration event. A cycle of assembly and disassembly of SEC62/63 from SEC61 may govern the activity of the translocase.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterotrimeric complex composed of SEC61-alpha, SEC61-beta and SEC61-gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376656.1">
        <property type="nucleotide sequence ID" value="XM_007376594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS29"/>
    <dbReference type="FunCoup" id="G3AS29">
        <property type="interactions" value="973"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AS29"/>
    <dbReference type="GeneID" id="18874342"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62485"/>
    <dbReference type="eggNOG" id="KOG1373">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031763_2_1_1"/>
    <dbReference type="InParanoid" id="G3AS29"/>
    <dbReference type="OMA" id="PMMRQMF"/>
    <dbReference type="OrthoDB" id="420669at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005784">
        <property type="term" value="C:Sec61 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005048">
        <property type="term" value="F:signal sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070843">
        <property type="term" value="P:misfolded protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3370.10:FF:000002">
        <property type="entry name" value="Transport Sec61 subunit alpha isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="entry name" value="SecY subunit domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019561">
        <property type="entry name" value="Translocon_Sec61/SecY_plug_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006341">
        <property type="entry name" value="PRK08568.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10559">
        <property type="entry name" value="Plug_translocon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00756">
        <property type="entry name" value="SECY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Translocon Sec61/SecY plug" evidence="15">
        <location>
            <begin position="41"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF10559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW31878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="479" mass="52617" checksum="FAE50E0DF1CF2253" modified="2011-11-16" version="1">MSGFRVLDLVKPFSPFLPEVIAPERKIQFQQKVMWTIITLLIFLVMSEIPLYGIVSSDSSDPLFWLRMMLASNRGTLMELGISPIVSSGMLFQLLQGTKLISVDMSNKTDRENFQVGQKLLAILLAVGQATVYVLTGMYGPPKSLGVGVCLLLILQLVFASIIVILLDELLQKGYGLGSGVSLFTATNTCEQVFWKAFAPTTSTSAKGTEFDGAVVAMFHLFGTRKDKRRALIESFYRPNLPNMFQVFATLFVFFAVVYLQGFRIELPIKSTKQRGSYGVYPIRLFYTSNIPIMLQSALSSNVFIISQLLFMRWPSNLFVKLLGSWEPRPGSSQLYANSGIAYYIQPPFSLTEALLDPIKTTIYIAFVLVSCAVFSTTWIEVSGTSPRDIAKQFKEQGLVIAGHRETSAYRELKKIIPTAAAFGGATIGALSVACDLMGTLGSGTSILLAVTTIYGYYELAAKEGGFSKNLVSGFSDGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASK6</accession>
    <name>G3ASK6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BBC1/AIM3 cysteine proteinase-fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_62559</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62559"/>
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    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="AIM3_BBC1_C"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="331"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="175"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="494" mass="53646" checksum="5C467DF5771DD1AD" modified="2011-11-16" version="1">MKNSFMDIDVSKFGPPPPKLYRGPDEQLPIKPHATRESPPPPALPARRSETASPASGKVKPPKPHKPDAVKKTPPPKPMRKPSTLEETSPPPPYTEVDEKPKPKPPITKKPTFEAQQGDVSNELNNIFSRMKVKTESSPPVSEPDVAPKLVNKPAPPKPAHKPAPKPAPKPAPKPVVNVPNPVVNVPNPVPIANVPPVVNTPPAVNTPPVVPHSRESSASATPPPPPPPRNYNRAPVPTPPAVSTGPPELDLELHTGWYTSNGALKLPKSLAGKNYKTSYSFSGSRYTREITARLDDLSTITYKLEWDSSSSSNVKVTIANFIPSPLLTIPSKQELIDNHNRFGEYIASWCQHNYGQMVGRGECWDLAKEALSKGCGNHAFVSTGFTHGFPILQISNTGSGVVFNSPQLDEVRRGDIIQFKSCKFYNVTSGVTKTVGAPDHTSVVVGNNNGRIQVLEQNVNSVRVVVDGEYIFADLTQGDLTVYRPMPAEWAPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        <name evidence="9" type="ORF">SPAPADRAFT_62671</name>
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    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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    </dbReference>
    <dbReference type="STRING" id="619300.G3AT40"/>
    <dbReference type="GeneID" id="18874425"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62671"/>
    <dbReference type="eggNOG" id="KOG0007">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013259_0_1_1"/>
    <dbReference type="InParanoid" id="G3AT40"/>
    <dbReference type="OMA" id="HYASIDW"/>
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    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="SF3A1"/>
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        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15316">
        <property type="entry name" value="SPLICEOSOME ASSOCIATED PROTEIN 114/SWAP SPLICING FACTOR-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR15316:SF1">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 1"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF12230">
        <property type="entry name" value="PRP21_like_P"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="SWAP"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="SURP motif" evidence="8">
        <location>
            <begin position="17"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="SURP motif" evidence="8">
        <location>
            <begin position="103"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="270"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW30803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="392" mass="45118" checksum="8823DB54A14212CE" modified="2011-11-16" version="1">MSIPKDIVVPPKDVKETIDKTVTYVIKNGKSFEERLLSNNKDNKFDFILDGDPHHAYYSWKLSCETPKEQEVPTSDEDIVVDKPRDLSFLVDLPAISTHDLDIINTTALYIAQNGTDKIPSLLEHEAKLGKRAQFEFLNTSHSLHKLFQTYITQYKKVIDLYKSEQPLPSHTNKFEILTIAYDRAQYISQNKVKEKNQEQLKQQQQIHYASIDWQDFVLVGKIEFDAVDEVQELSVPLTRDELVTRSLESKSREVELPKVAIEVVPEEKEEILDKEEPEPNAAVPGPFKGMKIKAAGASRLKKSSTPKSQEQNIKCPITGKLIPESEFDNHLKSLLRDPRYKQQQENYIRKNFTYASNITTDQVYENIKRLVRKRTSEEPTTTTGKRIQNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT49</accession>
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            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_52010</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
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            <taxon>Dikarya</taxon>
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            <taxon>Pichiomycetes</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="37"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="421" mass="49010" checksum="B0D3FE30343F629E" modified="2011-11-16" version="1">MSPISSYASERVNTENEQHIQPEVVQDNYKEPSNQKVWSLLDLPDEVLELITSYLNQLTVLEIGLTNSRMNNLATRKLYRNIYVNNGPSPILIARTLHTSFYIQYTVINNLKNFKQICSNLNMKHIHKVMFYSDAIFQDLIPLVCLLSTKVVLEFENKVSYDTIVFDHSDLYISKVLKVPPVSMPCINKFTVDCSTDVDLSILLYLPNLKTIEISKANSETISRFDAIKDISLKIKNFSISLLEMVDIRFIEKFFKLDQIIFFQLHICEPENFNYIFNMQLLLVFVRMKRLKHLALSNFPFETNNIISAIQSDLQSLFLRFNTEFEQTPFSVLQVIYSHKALARICLTIEEPVNEFWSLQDFGRVYKHESTKINVDCMETLDKFRLAVKHGLCPKLNQVILNQGYYSVERYSDGSSDFVKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATC2</accession>
    <name>G3ATC2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_156278</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376918.1">
        <property type="nucleotide sequence ID" value="XM_007376856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATC2"/>
    <dbReference type="GeneID" id="18871048"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_156278"/>
    <dbReference type="eggNOG" id="KOG3017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035730_8_1_1"/>
    <dbReference type="InParanoid" id="G3ATC2"/>
    <dbReference type="OMA" id="NPGHYEQ"/>
    <dbReference type="OrthoDB" id="337038at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP" evidence="1">
        <location>
            <begin position="13"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="145" mass="16468" checksum="EF0DE01EB4E540A6" modified="2011-11-16" version="1">MYDYKGPYALNKTFQEEMLLDHNRKRARHGVDPLKWSANCFNFASAYASQYDCSGKLIHSGGSFGENLAYGYTPLGAMNAWYKEGEEYIYGTESIYNHFTAIVWKSTTEVACAYRQCPRARYIICSYSPHGNVIGHSTMNVFAPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATI5</accession>
    <name>G3ATI5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine/threonine-protein kinase CBK1</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_72840</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. COT1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376980.1">
        <property type="nucleotide sequence ID" value="XM_007376918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATI5"/>
    <dbReference type="FunCoup" id="G3ATI5">
        <property type="interactions" value="563"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATI5"/>
    <dbReference type="GeneID" id="18875458"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_72840"/>
    <dbReference type="eggNOG" id="KOG0605">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_67_2_1"/>
    <dbReference type="InParanoid" id="G3ATI5"/>
    <dbReference type="OMA" id="KIINWQE"/>
    <dbReference type="OrthoDB" id="3638488at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902554">
        <property type="term" value="C:serine/threonine protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007118">
        <property type="term" value="P:budding cell apical bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030950">
        <property type="term" value="P:establishment or maintenance of actin cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050708">
        <property type="term" value="P:regulation of protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000920">
        <property type="term" value="P:septum digestion after cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd21773">
        <property type="entry name" value="MobB_CBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05629">
        <property type="entry name" value="STKc_NDR_like_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000086">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000828">
        <property type="entry name" value="Serine/threonine-protein kinase CBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000192">
        <property type="entry name" value="Serine/threonine-protein kinase cot-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF76">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_135, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="323"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="637"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="107"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="107"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW30947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="719" mass="83355" checksum="0AD828658F0E5344" modified="2011-11-16" version="1">MNFDELTEAQKQQLYYQHLQQQQQQQQQQQQQQQQQQQYQQQYHQNAQQQQYPQFTQQQAYPQMNQYAQQPNIVYQQQQQQQQQQISQQYAQYDDSYMDEDTSQILDQPPTVQDQNSPVRRQQEPTVPLSSTSPRFNLGFDNASPFTNTNNDSFENGKFTSIDSLNFRPPTSAFTQLNNNSHTNLSEISSNSATNSLVSHHSNQNVGSPVITNSTIPSGITQPQAAPTPAAKNTVNIVRQPNLLTKNSQEKAASIKLTLENYYTMSVNHAIERNQRRLDLEHKLLNEESGSSEERKNRQLQNLGKKESQFLRLRRTKLALEDFNTVKVIGKGAFGEVRLVQKKDTGKIYAMKTLLKSEMYKKDQLAHVKAERDVLAGSDSPWVVSLYYSFQDSQYLYLIMEFLPGGDLMTMLIRWQIFTEDITRFYMAECVLAIEAVHKLGFIHRDIKPDNILIDIRGHIKLSDFGLSTGFHKTHDSNYYKKLLEKETPNTQHLQPQTANGARNSVMVDAIHLTMSNRQQMQTWRKSRRLMAYSTVGTPDYIAPEIFIHQGYGQECDWWSLGAIMFECLVGWPPFCSETPHETYRKILNWQETLQIPEDIHLSPEAEDLIRRLLTSSENRLGRYGGAEEIKQHPFFRGVDWETIRRVDAPFIPKLRSITDTRFFPTDELENVPENPALSKAMQQREMDAKTGKKNLKEDLPFIGYTYSRFDYLTRKNAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATJ9</accession>
    <name>G3ATJ9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Cap-associated protein CAF20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_62869</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Acts as an inhibitor of cap-dependent translation. Competes with eIF4G1 and EAP1 for binding to eIF4E and interferes with the formation of the eIF4F complex, inhibiting translation and stabilizing mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the CAF20 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376995.1">
        <property type="nucleotide sequence ID" value="XM_007376933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATJ9"/>
    <dbReference type="FunCoup" id="G3ATJ9">
        <property type="interactions" value="362"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATJ9"/>
    <dbReference type="GeneID" id="18874500"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62869"/>
    <dbReference type="eggNOG" id="ENOG502S2E7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128343_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATJ9"/>
    <dbReference type="OMA" id="NGNERPY"/>
    <dbReference type="OrthoDB" id="3995390at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008190">
        <property type="term" value="F:eukaryotic initiation factor 4E binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031456">
        <property type="entry name" value="Caf20"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17052">
        <property type="entry name" value="CAF20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0810">Translation regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="61"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW30962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="164" mass="18939" checksum="1592C206C397F9AB" modified="2011-11-16" version="1">MVKYTEEQLLEAREEAYVPSPQILEAFNQMIDQVKEHAAAEYEKHKALKWSNGDTYIDEHGHERSYHHMNRRRASRTGGSKPNLRKKSEVDQDGWETLSKPKKSFGAEEGVEERNKFRETIKARPNNKNLGSSKPVDHREIIADKPVKAFNAFAALEDDDDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATQ3</accession>
    <name>G3ATQ3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_63126</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377250.1">
        <property type="nucleotide sequence ID" value="XM_007377188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATQ3"/>
    <dbReference type="GeneID" id="18874606"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63126"/>
    <dbReference type="HOGENOM" id="CLU_2623560_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATQ3"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="78" mass="9178" checksum="09A5B009F82BE935" modified="2011-11-16" version="1">MPLNIRGRKIEKSIPYAGFSDMEHYGDIYIIGAIVFVTSQHRFKPGWKLRRYVYIRSLFPGICRVYIRGTSTRRSCYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATT7</accession>
    <name>G3ATT7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_157168</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377283.1">
        <property type="nucleotide sequence ID" value="XM_007377221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATT7"/>
    <dbReference type="FunCoup" id="G3ATT7">
        <property type="interactions" value="215"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATT7"/>
    <dbReference type="GeneID" id="18871068"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157168"/>
    <dbReference type="eggNOG" id="KOG0922">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001832_5_10_1"/>
    <dbReference type="InParanoid" id="G3ATT7"/>
    <dbReference type="OMA" id="APVHDFV"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000028">
        <property type="entry name" value="ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002124">
        <property type="entry name" value="ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000145">
        <property type="entry name" value="probable ATP-dependent RNA helicase DHX40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF118">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="186"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="373"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="110"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="832" mass="94639" checksum="DD58484DC8389C37" modified="2011-11-16" version="1">MTKDVLSGTVNNSSDSAGLKKRKRKRSKKTHQEEPQVQPPQPQQNDLAQDKKRVNKKLKFEEEETDVVVSESPIEREQPESGHIQELNNSHTHKKTDTHKHKHKHLEEVEHEHKHEHQHEHKHEHKHKQKHKPKVVKFTEKEEEDDEDDFSNKFTFNTTKQLTERANELLQIRKQLPIYKHHDKILSCINSNQVTIVIGETGSGKSTQIPQFLMNESMKMIGVTQPRRVAAASLAARVSEEHGCKLGQDVGYQVRFTNMTSNKTKLKYLTDGMLLREIMLDNKLTKYSIIILDEAHERTILTDLIMGFLKSLLLSGERPDLKVVIMSATLNAELFSKFFNNAPVLFIEGKMYPVSQYYLDDDSSDIVDTMIRTIIQVNLSEPEGGDILCFLAGQEEIDNCVKVLQDIAPQLPKQAPLIVPLPLYAALSPNQQSKIFESLPKHQRKVILATNIAETSITVSGVKYVIDSGLRKVKVWKHNLGLSTLLTTPISQASAKQRAGRAGRESAGKVFRLYSEPVFSTLPQQQESEIKRNDIILPVLTLKKLGIDDLLNWTWLEYPGQDSILNALNSLYTLGALNDQGKVTSLGYKMSILPLTPQLSVVLLTAVELGVVSEVIDIVSCLSVDNLVLNVTEEARDEINFKRRNYCPLGIQYGDLISMYEFFNNFQKLSPGEAKEWCHEMMFSYKGFKNVIRVKNQLKEYMYTTIKQDDSLTNDQKDMELSKLKAQFEKEDEEDGVTEGINIPNILKSFLKGYITNTAVGMPDRSFRTFNTGQLISIHPSSTLFGKANIDAIMYIEYVYTMKSYARNCSIIELAWLQEIAPNIISKININE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATW0</accession>
    <name>G3ATW0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hsp70 nucleotide exchange factor FES1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Hsp70 nucleotide exchange factor fes1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_63190</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FES1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377306.1">
        <property type="nucleotide sequence ID" value="XM_007377244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATW0"/>
    <dbReference type="FunCoup" id="G3ATW0">
        <property type="interactions" value="209"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATW0"/>
    <dbReference type="GeneID" id="18874634"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63190"/>
    <dbReference type="eggNOG" id="KOG2160">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046722_1_0_1"/>
    <dbReference type="InParanoid" id="G3ATW0"/>
    <dbReference type="OMA" id="LHWSIAN"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071629">
        <property type="term" value="P:cytoplasm protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050693">
        <property type="entry name" value="Hsp70_NEF-Inhibitors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013918">
        <property type="entry name" value="Nucleotide_exch_fac_Fes1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316:SF18">
        <property type="entry name" value="HSP70-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316">
        <property type="entry name" value="PROTEIN FOLDING REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08609">
        <property type="entry name" value="Fes1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nucleotide exchange factor Fes1" evidence="5">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="281" mass="31627" checksum="2B4F1F6B7C604165" modified="2011-11-16" version="1">MDKLLQWSIAQQSGDQEAMARIGQPDEKMLQQLFGGPDEPTLMKQSITLVNDPEVTLENKEIALENFEMLIENLDNANNIENLKLWPSIVNLLDPTSPESLRLLACSIIGTAVQNNPKSQEDFSNTEGINHLIQLAHSDTNKQIQLKALYAISSFIRNYKPAYTQFEQYQGWNIIQSDTTDSKRIIRVLSIVSSILSNGIDTNTHQQIKQQGLPGLLVSVLHKDSNVNLLDKAINIIVELHNHKYEFTDEEISSIKAAIQDISNVEHLNKDDLDAVTNIWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATZ1</accession>
    <name>G3ATZ1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable NADPH dehydrogenase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Estrogen-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_63219</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Oxidoreductase that binds mammalian estrogens with high affinity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377338.1">
        <property type="nucleotide sequence ID" value="XM_007377276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATZ1"/>
    <dbReference type="FunCoup" id="G3ATZ1">
        <property type="interactions" value="852"/>
    </dbReference>
    <dbReference type="GeneID" id="18874648"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63219"/>
    <dbReference type="eggNOG" id="KOG0134">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012153_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATZ1"/>
    <dbReference type="OMA" id="YMANAVN"/>
    <dbReference type="OrthoDB" id="276546at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042562">
        <property type="term" value="F:hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02933">
        <property type="entry name" value="OYE_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000138">
        <property type="entry name" value="NADPH dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF91">
        <property type="entry name" value="NADPH DEHYDROGENASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="7">
        <location>
            <begin position="14"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00056646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00067604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00075326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="416" mass="46813" checksum="02582534571AD98A" modified="2011-11-16" version="1">MTTEELPPLAESNVFKPIKVGNHTLSNRIVYVPTTRFRAAKGDPKTRHVPSDLMQQYYSDRAQYPGTLLITEATLVSDRAGGYEGVPKISTKEEVDGWKKITDKVHERGSFISCQFWFLGRVADPKVLKERGLDMIAPSAIYPSEEAKAAAEKQGVQVRAVTEEEIKDIILNDYTIAAKNAIAAGFDYIELHGAHGYFIDQFLHENSNTRTDKYGGSIENRARFALEVIDHLIGVVGADRLAIRLSPWAEVQGIEERASPIPTFSYLLDQLQRRADKGNKLAYVSVVEPRVQGTVTVDASSAKGDNHFVEQVWKGIIVRAGNYTYDAPEFKSIREDTANGRTLVGFSRYFTSNPDLVSKLKNDPSTLVKYNRATFYDASNWGYNTYRNHGDETKYDEAVEKKRYPKVIDDRREANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUA1</accession>
    <name>G3AUA1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_63300</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377448.1">
        <property type="nucleotide sequence ID" value="XM_007377386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUA1"/>
    <dbReference type="GeneID" id="18874692"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63300"/>
    <dbReference type="HOGENOM" id="CLU_2139536_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUA1"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="113" mass="12945" checksum="2ADDB5B1BAC8EEAC" modified="2011-11-16" version="1" fragment="single">MNFSQIRISIEYGDGANYVLKYIKDIDISLATKFSLDRNNYNFFSDAIIENNYSIKELVISDNIFANFSLDIIPKLKGLEILIISSFTQEVLDSLHEFGVSKLQLKKLVLKNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUF0</accession>
    <name>G3AUF0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_63360</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377497.1">
        <property type="nucleotide sequence ID" value="XM_007377435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUF0"/>
    <dbReference type="FunCoup" id="G3AUF0">
        <property type="interactions" value="275"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUF0"/>
    <dbReference type="GeneID" id="18874717"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63360"/>
    <dbReference type="eggNOG" id="KOG2742">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_3_1_1"/>
    <dbReference type="InParanoid" id="G3AUF0"/>
    <dbReference type="OMA" id="IWVGMDE"/>
    <dbReference type="OrthoDB" id="64915at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840:SF5">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase C-terminal" evidence="2">
        <location>
            <begin position="145"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="37852" checksum="D1822D4770E228E9" modified="2011-11-16" version="1">MSDKINFAVVGTGIFATDTHLPTIQKIPELNPAAAYNRTKSKAEQFATKAGIPQDKVYDTLEEAFQDKTIDFVDALLPVQFNLDAVKLAIEHNKPLAFEKPIAANLTQAKEIVQLSEKTEVPIMILENWTYLSAIDILKNQVLPKIGEVVAFTYNATGPFHDTSKYLATGWRQKPEHIGGFLSDGGVHQLALLTEVLGEVESVSGLTKQLREQSGADDILFSTFKLKSGVIGTFTYGSAFGATDKSTSFTIFGTEGSAVYDWSPSLSKPTVTYQTGSTSSTASAKSSIEIDEINTIEAEFKNFRDVVKANDKKLVKVTPRKAFHHLAIVAAALESSKKNGTSVNVEIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUT8</accession>
    <name>G3AUT8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_158376</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.67" evidence="1"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377794.1">
        <property type="nucleotide sequence ID" value="XM_007377732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUT8"/>
    <dbReference type="FunCoup" id="G3AUT8">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUT8"/>
    <dbReference type="GeneID" id="18871122"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_158376"/>
    <dbReference type="eggNOG" id="KOG2283">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020105_4_1_1"/>
    <dbReference type="InParanoid" id="G3AUT8"/>
    <dbReference type="OMA" id="YIESWYR"/>
    <dbReference type="OrthoDB" id="16692at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016314">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043491">
        <property type="term" value="P:phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051896">
        <property type="term" value="P:regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14497">
        <property type="entry name" value="PTP_PTEN-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051281">
        <property type="entry name" value="Dual-spec_lipid-protein_phosph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305">
        <property type="entry name" value="PHOSPHATASE WITH HOMOLOGY TO TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305:SF81">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 3-PHOSPHATASE AND DUAL-SPECIFICITY PROTEIN PHOSPHATASE PTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatase tensin-type" evidence="4">
        <location>
            <begin position="16"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="3">
        <location>
            <begin position="103"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="334" mass="38940" checksum="97A563CAF1CF3EB1" modified="2011-11-16" version="1">MKSIIRSIVSSPKRVHYDKPLGLQYDLSYITSQIIVASAPVSGFLQQYYRYPLSDLVTFLNTYHLNHWRLFNFRGEDPGYTDEEVNGQVSHYPFPDHSPPSFDIMLQCVKELQQYLKQDTKNVAVLHCKAGKGRSGSLCCAYLMYDEFSNGRGFNVKSVIDLFTEKRMQSFAGDGISILSQQRYLDYWSTFLNSTPELRQQYLEYVEQVKPYEIYKVRVKNVCNLSEFYKLNLLLNTVAKFVKKLTAENSKMTKHDQNLFFTPNTPIKLDSSMLDIKLGVKTWCYCWFNIYFESLASSPSVILQWEDLDGFKGTRQRGVKVFDEIEVYWNIPQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUX9</accession>
    <name>G3AUX9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Allantoate permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_158460</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377836.1">
        <property type="nucleotide sequence ID" value="XM_007377774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUX9"/>
    <dbReference type="FunCoup" id="G3AUX9">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUX9"/>
    <dbReference type="GeneID" id="18871127"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_158460"/>
    <dbReference type="eggNOG" id="KOG2533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_2_2_1"/>
    <dbReference type="InParanoid" id="G3AUX9"/>
    <dbReference type="OMA" id="TFVAMLQ"/>
    <dbReference type="OrthoDB" id="1935484at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000247">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000106">
        <property type="entry name" value="MFS transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF29">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="320"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="457"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="492"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="550" mass="62934" checksum="7F137DFA22FA49D0" modified="2011-11-16" version="1">MSASSINSIERVNEVTTEKKEDYTITALDSQSSLESDLLAKNPFLDPKVEEYYRQVYSESKYESYSAFDPHFTWTPEEEKKLVRKLNYRVAAAACLMFVGLQVDRGNLAQAVTDNLLADLNMDTNDYNTGNTIFLVAFLLAEVPSQLISKALGPDIFIPIQICLWSVVAMSQAALSGRTSFFITRAIIGALEGGFIADLVLWLSYFFTSKELPVRLSWFWTTLSLVGVFTSLLAFGLLRMRGVAGWAGWRWLFLIEGIFTLAIGIGSFYMMVPSAVQTKNFLRPKGWFTEREEKIVVNRVLRDDPSKGTMHNRQGLTIKMLFKSLIDFDIWPIYAIGILAYIGQNTFGSYFGLINKQLGFSTFNTNLLAIPYQVIHIIFLLAITWFSERINERTLTCIIAPLYATPLIALIRWWPQAGKNVWGTWTLNTLYLGQPYIHAICVAWVSRNSNSVRTRSVSSAIYNMFVQLGNVAANFIYRKDDLPLYHRGNMQLFAITFALIPLLILVKVYYIYRNKHKRQLWDAMTPDEQEYYRNNTTDEGARRLDFFFAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV04</accession>
    <name>G3AV04_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inheritance of peroxisomes protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_52913</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for peroxisome inheritance.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the INP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377844.1">
        <property type="nucleotide sequence ID" value="XM_007377782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV04"/>
    <dbReference type="STRING" id="619300.G3AV04"/>
    <dbReference type="GeneID" id="18871735"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52913"/>
    <dbReference type="eggNOG" id="ENOG502S7ZC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_575114_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV04"/>
    <dbReference type="OMA" id="YWKIFIN"/>
    <dbReference type="OrthoDB" id="4097008at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005780">
        <property type="term" value="C:extrinsic component of intraperoxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045033">
        <property type="term" value="P:peroxisome inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024758">
        <property type="entry name" value="Inp1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12634">
        <property type="entry name" value="Inp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="475" mass="53530" checksum="3C72A968A277B7A7" modified="2011-11-16" version="1">MVPSEPNTPKKSVNNSNNTNADKKKRRKSKQKKRKSSIPTTTVSQTQLGPSVLASPRQQTLMRHKLDDDSDKSHPVTLLKQLKRHTPPQEVEPDISMPDIAHKLTLFKYKTGTIIQFLDQIKHNSSGTLLAQGEIEIFQLHNGDVTYLSCGKSFIYPLLPKIKILRASFNQFIIPLVNPERYWRLSVTTEETNVVDLLETTFEKYAQYRNLYVSGQRSISPQTLVQASQLTPVQAKSHFTLDDTIPESPPSAPISPRQEAISPPRFALHDPIPSHLIHHPTPRVNPSYNPFHPPIPQDNNIDNKSDLDSLLDEYEQTLEATTRANSIVHEGRSRRSSISELYTQESGWMEPTPAPAPQRTTAPVTRGIPKSRSIYSMNSYRSYDLNHIYNTVRHGNQEREELKSAKSMSKLPHDYQTRLSSRRSSAIAHPLVPSAPVAPTSATTQLNSKEIFNMLSSKPTPPRTTSFAARLFGWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV15</accession>
    <name>G3AV15_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_68915</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377854.1">
        <property type="nucleotide sequence ID" value="XM_007377792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV15"/>
    <dbReference type="STRING" id="619300.G3AV15"/>
    <dbReference type="GeneID" id="18875246"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68915"/>
    <dbReference type="eggNOG" id="ENOG502RQGZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028681_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV15"/>
    <dbReference type="OMA" id="DEICATS"/>
    <dbReference type="OrthoDB" id="4016300at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="605" mass="69573" checksum="5B15931F48D9A658" modified="2011-11-16" version="1">MSECLRLFSQEFENLIQRTNSKPIITYEEFDFNQLINYLEQLDILNKLQQHLSESMSSEEAQSIDKIKTLLDKLFTIARIQDFLSDELSYFIVNLHLSEFIHTHFPREMEQYTSILMKISIYNLVEDSYLSNLLDDSLSSKSRAWLSTNILINTAHNFNVSTYINMVNPKNHAKFQKLLQENSDFTNIICLMTLFKKLKVDHLFLAGLPQSFLPMDQIKYITNYNPSFRNLISCSITSKAIILPDCPDCFIQPKESKSLVLWMNDKKSFIPCLINLHTLSNYLALSDSTLRIKFECMNSRRTRSGSLSNPEYLQSFSIVKERTEFLTDIEIELKLQQQSQFNWLVSYLSQNKSNKTNKTRKISINSLIHVSESQVMPDTNETTDSLVTDEVITSSVLTRTNSTDTHSAEITSLEKNEETVPKRSPVRSSGSIRKRPKSSVKTPQNHLKSELAKYRGIDQAKEMVQPISTQDILLATNSQVSKRLNNYNLPGAANKQLPISTVSDEICATSTANNTILPREFTDIDLNQTTCPTIGSAYDVMNESLKLLSSNLINKLKSLEFNILKKQNQLQQELDNAFAKIEHQHKIKLQEIQQYYKQECEKILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVD2</accession>
    <name>G3AVD2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PSP1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_52978</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377917.1">
        <property type="nucleotide sequence ID" value="XM_007377855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVD2"/>
    <dbReference type="GeneID" id="18871745"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52978"/>
    <dbReference type="eggNOG" id="KOG4679">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019022_1_0_1"/>
    <dbReference type="InParanoid" id="G3AVD2"/>
    <dbReference type="OrthoDB" id="243127at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047767">
        <property type="entry name" value="PSP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007557">
        <property type="entry name" value="PSP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43830">
        <property type="entry name" value="PROTEIN PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43830:SF3">
        <property type="entry name" value="PROTEIN PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04468">
        <property type="entry name" value="PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51411">
        <property type="entry name" value="PSP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSP1 C-terminal" evidence="2">
        <location>
            <begin position="388"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="507"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="548"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="569"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="596"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="636"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="669" mass="75366" checksum="5033D99E24346D4A" modified="2011-11-16" version="1">MTLNESGHHQHHTPDNTGNNYSNYNTSPSVAATNRHRHSHSAMFPPARSSPTQIGSSPPQAAVTFNTFTPLMPPQTQTSGSNYLRNNNNSNNQQTNPGNIWNNSNGSIPHVQRRHSAIDANIWSQGPTSTFQPTTNIWSSSGPSPNQENYTSSLSNRSSSISGFTPPPLEEQPNQQKQAIMNFQSGSPSQLLDPGRRHSFAESLPTTNSITAGSNNGTVSILNGGIDSNQTLKLVDEYFETDPHERVKVTLRLLNERFFDEEKYLGDAYQLPKFPIESSLRNYQLILVGFKAGRIDVFYLPSNNPPNSDLNRLKVGDLVIVEADRGRDLGKVFKMNISIDEARLLKLLQFQEQQAALNEHDNILDDLSVKNLSDSSNSVAPPTLHFPKSIISLAQPNEILQILNKKQDEEKACRLCLAKIANATSSQQLMGANSSATQDLLQMKLIDAEYQFDRKKLIFYYSTSKRIDFRDLVRELFRIYKTRIWMCAVIGLPYNVNRKSVSPLMNGATSNPFMQTQVPTQNPPQPQQQPQQQPQQIAFSRGFERRLSYQPPPTFQQYPNVANPPPHLQQAQQQQQQQQIPHPFQDGIPRRYSDSRPFSQQSQYAMFQGQQMQASPMNQPQLSPFQTDFQFPPPHPDNRNEGTIREEEDLNNNSGEAFVLKSLVDSINH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVL2</accession>
    <name>G3AVL2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Protein transport protein SEC23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">SPAPADRAFT_144148</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15 16">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 16">Cytoplasmic vesicle</location>
            <location evidence="1 16">COPII-coated vesicle membrane</location>
            <topology evidence="1 16">Peripheral membrane protein</topology>
            <orientation evidence="1 16">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 16">Endoplasmic reticulum membrane</location>
            <topology evidence="2 16">Peripheral membrane protein</topology>
            <orientation evidence="2 16">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Golgi apparatus membrane</location>
            <topology evidence="16">Peripheral membrane protein</topology>
            <orientation evidence="16">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the SEC23/SEC24 family. SEC23 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377727.1">
        <property type="nucleotide sequence ID" value="XM_007377665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVL2"/>
    <dbReference type="STRING" id="619300.G3AVL2"/>
    <dbReference type="GeneID" id="18870603"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144148"/>
    <dbReference type="eggNOG" id="KOG1986">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008658_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVL2"/>
    <dbReference type="OMA" id="LFHGERE"/>
    <dbReference type="OrthoDB" id="10256289at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000041">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037364">
        <property type="entry name" value="Sec23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141:SF0">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 16" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12 16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 16" id="KW-0653">Protein transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0813">Transport</keyword>
    <keyword evidence="9 16" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="18">
        <location>
            <begin position="58"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="19">
        <location>
            <begin position="121"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="21">
        <location>
            <begin position="428"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="20">
        <location>
            <begin position="547"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="17">
        <location>
            <begin position="669"/>
            <end position="758"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU365030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="EGW29961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="813" mass="90568" checksum="66EBB973A92AB429" modified="2011-11-16" version="1">MDFEAREDQDGVRLSWNRLPKSKLQHDRNIIPLGALYTPLNNKADPMVPLLEDPSYLVSCRGCRTILNPYVFINNEIWTCPCCSSSNQLPPLFDVNGVPQLHPSVTPEYSTVEYKTGKQAPLPPIFFYVVDTCFEEGDQDALAQLKESLVVSLSLLPEDALVGFISYGKHVRIHDLANGDNVSYGFNGSKQYTLEQIQASLGMLSTGLSSAGAASAGATHASVDTILGVVGKKFLTPVNIGEYQLTRIIESLTPNLFPRDEFTQRPERCTGSAINIASLLLRAILGNHIITTGGHLLVFSSGVCTVGPGKIVDQALKEPMRSHNEIEKSYHSMLPTTSTASSNTKSNISLFQNAKKFYKEVTKNLVKLGLSCNYFIGSYDQIGLFEMDEVCYKTGGVVVMSDSFTTTIFKQSFLRFFKKDQDEYLDMGFNATLEIKTSPDLKIEGLLGNATALPFNKTITHNSRMFSIHNDSKIGESGTNSFKLCNVNPQSTYAIFLEKLDSSGGASTIQFLFHYQHPSGEMRLRVTTVALPIVADSDTLNLEPGFDQEAALVLIARKSIYKLSPECSNQITTEVLLKQLDQLLVDFCARFAVYTMAQPESFRLSSIYNLLPQFIYHLRRSPFINVFGNSPDETSYIRHLFMHEDTSNSLIMVQPTLLSYDINDWNDENTEPIPVMLDSLSLGPSKILLLDTFFQILIYHGSQIAAWRNAGYHEQADFAYFKDFLEAPKQEAMILLMDRFPLPRFIDCDENGSQARFLMAKLNPSTNYASNVNQFVGGGVGGTSSSSPYDVLTDDLNLQTYMEHLQRLVVNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVT0</accession>
    <name>G3AVT0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_63601</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377741.1">
        <property type="nucleotide sequence ID" value="XM_007377679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVT0"/>
    <dbReference type="STRING" id="619300.G3AVT0"/>
    <dbReference type="GeneID" id="18874826"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63601"/>
    <dbReference type="eggNOG" id="KOG4208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025741_0_1_1"/>
    <dbReference type="InParanoid" id="G3AVT0"/>
    <dbReference type="OMA" id="WKGANKR"/>
    <dbReference type="OrthoDB" id="21467at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12307">
        <property type="entry name" value="RRM_NIFK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46754">
        <property type="entry name" value="MKI67 FHA DOMAIN-INTERACTING NUCLEOLAR PHOSPHOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="122"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="26"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW29975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="268" mass="31032" checksum="09B6AC7918250CD7" modified="2011-11-16" version="1">MAKSVKKTQSKKAAPKKETVEKSVEQIEEDLQLPSSSDEEEQAADEEEEEEPLSSEDDDDDDDEDLHGLSSDDEDEEEQEEQEEQKEQQQPEEKSKSRHTVNKSVESSSAAKSNKKKKSKRGIMYIGRLPSGFQEQELTKYFTQFGDIINLKLSRNKKTGKSKHYGFIEFESYEVAKVAAETMNNYLLFGHLLKCQVLENNDEIHQDLFKDANKKFKVIPWGKISKHKHDKPKTKEQWEVLVKKFEQSKKNKQNELKSKGIDFDLNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEE3</accession>
    <name>G3AEE3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein transport protein SEC23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">SPAPADRAFT_58928</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 15">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 15">Cytoplasmic vesicle</location>
            <location evidence="1 15">COPII-coated vesicle membrane</location>
            <topology evidence="1 15">Peripheral membrane protein</topology>
            <orientation evidence="1 15">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 15">Endoplasmic reticulum membrane</location>
            <topology evidence="2 15">Peripheral membrane protein</topology>
            <orientation evidence="2 15">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Golgi apparatus membrane</location>
            <topology evidence="15">Peripheral membrane protein</topology>
            <orientation evidence="15">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the SEC23/SEC24 family. SEC23 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373143.1">
        <property type="nucleotide sequence ID" value="XM_007373081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEE3"/>
    <dbReference type="FunCoup" id="G3AEE3">
        <property type="interactions" value="1054"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEE3"/>
    <dbReference type="GeneID" id="18872685"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58928"/>
    <dbReference type="eggNOG" id="KOG1986">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008658_4_0_1"/>
    <dbReference type="InParanoid" id="G3AEE3"/>
    <dbReference type="OMA" id="MPWNIIP"/>
    <dbReference type="OrthoDB" id="10256289at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902953">
        <property type="term" value="P:positive regulation of ER to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070863">
        <property type="term" value="P:positive regulation of protein exit from endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003400">
        <property type="term" value="P:regulation of COPII vesicle coating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11287">
        <property type="entry name" value="Sec23_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.730:FF:000001">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000006">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000043">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037364">
        <property type="entry name" value="Sec23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037550">
        <property type="entry name" value="Sec23_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141:SF0">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 15" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="11 15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 15" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <keyword evidence="8 15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="17">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="19">
        <location>
            <begin position="227"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="18">
        <location>
            <begin position="339"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="16">
        <location>
            <begin position="452"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU365030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EGW35731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="585" mass="65734" checksum="784A6209E8311658" modified="2011-11-16" version="1">MVQVHDLGSEKINKSYIFRGDKEYTDKQISEMLNRPLAPSPAGQQPQFANSLTRFFLPAEEVEFQLTSLLENLNGDPWPVANGDRALRCTGSALNVASNLLGLTYAGFGARIMLFTAGPCTLSPGLIVGPQLKEPIRSHSDIDKDNAKHFKKATKFYDSLAARAVKNAHTVDIFAGCYDQIGMLEMKNLCNLTGGSLLLTDSFTTAIFKQSFLRLFNKDSQGFLNMGFNGTLDIKTSRELKVSGVIGHASSLNVKSANVSENELGLGGSSQFRLCALSPRHTYGVFFDVVNTAQLPQNSQSYIQFITHYQHCSGSYRIRVTTISNFLTSDEQTLTNSFDQEAAAVLMARVTLFKAEQDDGADVLRWVDRMLIRLCQKFADYRKDQEESFRLGPQFTLYPQFIYYLRRSQFLQVFNNSPDETAFYRHVLMTEDCNNSLIMIQPTLTAFSLDSEPEAVLLDSVSIRDDRILLLDTFFHILIFHGRTIAQWRRAGYQDQPEYENFKELLQEPKVEAAELLVDRYPLPRFVDTEEGGSQARFLYSKLNPSVTYNSSEFVSNSGAIVLTDDVSLQVFMSHLQKLVVSGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEG8</accession>
    <name>G3AEG8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ORC6 first cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_145126</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ORC6 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372142.1">
        <property type="nucleotide sequence ID" value="XM_007372080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEG8"/>
    <dbReference type="FunCoup" id="G3AEG8">
        <property type="interactions" value="221"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEG8"/>
    <dbReference type="GeneID" id="18870671"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_145126"/>
    <dbReference type="eggNOG" id="ENOG502QS52">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060403_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEG8"/>
    <dbReference type="OMA" id="ARYHICA"/>
    <dbReference type="OrthoDB" id="5367324at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008721">
        <property type="entry name" value="ORC6_cyclin_first"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016811">
        <property type="entry name" value="ORC6_fun"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05460">
        <property type="entry name" value="ORC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF022941">
        <property type="entry name" value="ORC6_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ORC6 first cyclin-like" evidence="7">
        <location>
            <begin position="10"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="99"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="171"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="374" mass="43136" checksum="77F39BEFB1F14A0C" modified="2011-11-16" version="1">MSTIQLKRSLRDVLPTYQGEFPPKLLDYIASLYQLSLRKQGVLPNKAEIARYHLCAYSVAEKYQEKFDLPSPQITRIPIQPKLVGKLLDDFREFIDQVSAASSPNTSPRKRRADVEDPVTPTKGSKVVPGQFTKSAPKVGSPLKRLQQLQKEDTPGSKKQKTGVLKDIESPFNQKTTSPSKSPFNKKNNREGSPSSPHVYKYDKKNVSISDFITFCNNFYIPAQTSAKMTETFLMHKHKFVKKSEWLLAAAMVRTAYIRINHKLLSSKVGEEQKFLDLLFQYQKGGLMRWNLALWYDIVDDWMKDEQWLIDIEKQFMYGTETSQEMKVVTERRARIGEGWDLMERLGAMLHGDVLYESKPQQNYYDTWSSSILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEJ5</accession>
    <name>G3AEJ5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein HID1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_130767</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372169.1">
        <property type="nucleotide sequence ID" value="XM_007372107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEJ5"/>
    <dbReference type="STRING" id="619300.G3AEJ5"/>
    <dbReference type="GeneID" id="18869529"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_130767"/>
    <dbReference type="eggNOG" id="KOG2226">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_306764_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEJ5"/>
    <dbReference type="OMA" id="MIVYPLP"/>
    <dbReference type="OrthoDB" id="432953at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000138">
        <property type="term" value="C:Golgi trans cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026705">
        <property type="entry name" value="Hid-1/Ecm30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21575">
        <property type="entry name" value="PROTEIN HID1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21575:SF12">
        <property type="entry name" value="PROTEIN HID1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12722">
        <property type="entry name" value="Hid1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="891"/>
            <end position="916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="937" mass="106351" checksum="ADC381BAD193A7DF" modified="2011-11-16" version="1">MMTAEEKAKFRSHFLELGQGRIIPPTNTFWNTLWTYPTCAEDVYESLTPSDIRTVRNQNKVNFILLIRILCTKIVEYGDPVHIDKLSGTKHTVELLNCIRFVSKLVPFVFELAAYHNDLETVFFWSKEFDPIEYSRREAPVPLQPHEIHESLAVRLLSSLIKLLFVNGFTTSSKSMTVWEPGIGQRTKYDPPNLIIDSNRSELLKLLITLCSSPMYSSILHLSSTGSKFLTYLVSSTPKQELLFLVCSLVNVACRASRSSMDESSLIYPENIEYSEIRHVVVAYSFQLLTLLVAYPLPSPTETKFLVDAGLITSKPHNMARLYMGRIYKDNEILFLATSLVNILRFPVTGTRVTENGTKVKSTPPALWATEAAMLIWELMQCNKNFKELIGRKFVSELMVVLMYYTFSFHSNHVHANLVRVCTYLMLYLSSEDMINDLFFPMSSSLYESLPGPYKLQYHPLTTRDFLVCQICNCIIGKQANLLYLTNKPMPDLLAKTLVEILYNLIQPVSNDELNVHNDPLKKLNNPNSRGGLSYAAACSITHLIVKLSNRSFVLEIPFHSTSLALVIRAVATAAMNYPGPSRMLLFSMIKNEKVFQEVRNTMASLHGVHFKQGVPVRIDNTPEVVPVTPHLINGSFRPSIYGEPIETYGIARPINREEDHYMTEDETVAESLRPSSPRGMTNKAREKMKKNIPIGKSWGGDAALKTILEVIIPKLHEVLDESEENEILKMVQLIEDITFEDNYEKPEPLRFTWSHLSLGWYMSLLFHEIYDSNDLMKSFMRSNNGIFKSIGSSLASVSMFSYSWNSLLSRKPKTNQDEIQWVKNSLTNYNHWANTNVRLFKIDSGEGFFSSINKIGSGVSQSLPVSPVTEMATQFGKRFSELRLNSNQNRSSPVLSISAGETPPQRPDTLRKLSRNSISSLHTLNAINRTRSNTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEJ7</accession>
    <name>G3AEJ7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Origin recognition complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_145188</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication in an ATP-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12 13">ORC is composed of six subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the ORC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372170.1">
        <property type="nucleotide sequence ID" value="XM_007372108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEJ7"/>
    <dbReference type="FunCoup" id="G3AEJ7">
        <property type="interactions" value="425"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEJ7"/>
    <dbReference type="GeneID" id="18870673"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_145188"/>
    <dbReference type="eggNOG" id="KOG1514">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012774_1_1_1"/>
    <dbReference type="InParanoid" id="G3AEJ7"/>
    <dbReference type="OMA" id="FFNWPTY"/>
    <dbReference type="OrthoDB" id="1926878at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Origin recognition complex subunit 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Origin recognition complex subunit 1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AAA_lid_10"/>
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        <property type="entry name" value="ATPase_AAA_core"/>
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    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
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        <property type="entry name" value="BAH_sf"/>
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    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048867">
        <property type="entry name" value="WHD_ORC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF23">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17872">
        <property type="entry name" value="AAA_lid_10"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21312">
        <property type="entry name" value="WHD_ORC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82061">
        <property type="entry name" value="BAH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAH" evidence="16">
        <location>
            <begin position="49"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase AAA-type core" evidence="15">
        <location>
            <begin position="406"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="AAA lid" evidence="17">
        <location>
            <begin position="578"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 1 C-terminal" evidence="18">
        <location>
            <begin position="655"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="186"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="217"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00053599"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00062293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365058"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01426"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF17872"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF21312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW34758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="781" mass="88565" checksum="6D892292EE5365B7" modified="2011-11-16" version="1">MARSQKDLKGWQYILSEDNANPETPNRRSRRSARNTSSIILKRDNDDMELKVGDTVLVKDDNAVAEVALIKSIDFGTDYFIEVLVIWFTHADSKEPDVEQNEIYITPYLEKIQMKDVIMKVQVLSNEEYNDIVIDESNSSTTFMTRRACYDDGKSDSFDFRFLMDKFTENPDGFVQYLRTKTVVPTKKRKTSKNSSARASPAPVETIISKETSSVEAEAEAELETAEQDEEDEDEEEEEEEILDKDNDVDFEDLTGENSDDDVIGIDSSDDEYVDALQSPVKRSRGTPSARSSPVKSPRKSSKTTTPKSSPKKQQKNQIEELYSTILTPTKRRRVFKPNTPILPTLLSPSKNTNALTDPTSQAFKDIKAKLHTSQKLNALPGREDEYAMIYMNLESAVNEETGCCVYVSGVPGMGKTATIRDVIEQMTQSVERNEIKPFNYLELNGLKLVSPNVAYEMLWEQISGDKVSPASSALLLEEYFNREDNNRKPFIVLMDELDQIATKKQNVMYNFLNWPTYKNSKLIVIAVANTMDLPERVLSNKISSRLGLRRIQFRGYTFDQLGDIIRHRLDMLTKQSKRKVHISPDAIGFASRKVASVSGDARRALTICRRAVEIAEKEYSQEKNGNDDDDKPYQVQISHISAAINETVNSPLSQYLASLPYASKLLLAAVLLRSKRTGLAENTLGDIIDEMKNAQYKSNDTEITDILYADNLFDTSISKKGSRNLRVPYFKYILNSFVESGILIQQNVQGERQRLIQLNVPEEEVISVFKRDKQVSHLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEK7</accession>
    <name>G3AEK7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein MNS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">MNS1</name>
        <name evidence="2" type="ORF">SPAPADRAFT_57836</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372181.1">
        <property type="nucleotide sequence ID" value="XM_007372119.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="18872214"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57836"/>
    <dbReference type="eggNOG" id="KOG2204">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="InParanoid" id="G3AEK7"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:calcium ion binding"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="135" mass="15287" checksum="420FE782A3FF3E35" modified="2011-11-16" version="1" fragment="single">MSFSFGINNISRGNTYKDKPTGGTLPLYYKDKPNSFRPISHKKQRKLVMLLKGFIYSLILYTVYHFASTGNLNLFEFGFGGGIGSDNISKGATSWPAAQEEVKQAMLDSWHTYEKYGWGYDVYHPIKQQGENMGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF05</accession>
    <name>G3AF05_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_47888</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372221.1">
        <property type="nucleotide sequence ID" value="XM_007372159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF05"/>
    <dbReference type="STRING" id="619300.G3AF05"/>
    <dbReference type="GeneID" id="18871155"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_47888"/>
    <dbReference type="eggNOG" id="ENOG502RQ68">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_526741_0_0_1"/>
    <dbReference type="InParanoid" id="G3AF05"/>
    <dbReference type="OrthoDB" id="4026435at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="475"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="494" mass="55972" checksum="5C7542496813146B" modified="2011-11-16" version="1">MNGEQPSRESQPATLQSGYTSPSQPDTEPIPTSTPLHSPTIELSEPVGGTDNHLHVFQGAPDSVSPAFDPIEPANNNNDDDDNSNNNHHYKQPTSPDSDGILLSPPGMNKSYSYGGTITPPSGFGNANLSPPSDKLYKKQPRRESLAKKKDMLNNASGTNLNMFLRGDRRQSWTGAKPELMDSPKINSKLMMTSASNNTSLGTSSSMKSTISSGKLNGISDKNRLVDQFYRPNVKLHNRAHSSVDLASIFKSSIELTPGKRRWMNDSEMMESTSGSTLNHESALTDESFNEILNQGGFKFEFDREMLVQDDELINFVLNIDTILDEYPQDTNRPYEQLERIMTSMTDKLEIKFKNILLRHQPTKNKSLEELDSVEKYLHGLKLQTAELITQLNNNRELIRSKYREEINVNINKLTHVTAQLKGLEKRSNAFKEKITEENMVMSQDMIEKLELLENINLRMREYSRIRKARRFRRVQMLLSIGVVIFLAIYYGHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFL7</accession>
    <name>G3AFL7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_145755</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PER33/POM33 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372418.1">
        <property type="nucleotide sequence ID" value="XM_007372356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFL7"/>
    <dbReference type="STRING" id="619300.G3AFL7"/>
    <dbReference type="GeneID" id="18870700"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_145755"/>
    <dbReference type="eggNOG" id="KOG4002">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065417_1_0_1"/>
    <dbReference type="InParanoid" id="G3AFL7"/>
    <dbReference type="OrthoDB" id="5581259at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051645">
        <property type="entry name" value="PER33/POM33_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005344">
        <property type="entry name" value="TMEM33/Pom33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03661">
        <property type="entry name" value="TMEM33_Pom33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="283" mass="31619" checksum="494A6DD5467DA0F5" modified="2011-11-16" version="1">MGKKEAAKPAPAAKAPRFKWPAMPTMPAMPPMPAVNIPFKKDLFLSTLQSFQFVSFLGNFLTTLGVLLYTLTYFKLSSENYEVFHDISLVGVVISSGAFTVQNVIKHRLSLGPVIKDDSFHYLFIGLSLLILHPCVFFTVLPFGLYSVFIVLSFLTGSLFPIFEIENCFVAHLFNSITSGLRGPAVGFASGTELTAMVMLVLRVITFRSNSLLIVLIYAVFLKLRYETSQASRDAVKRFVNFGDKVIAIFDSHQMNNIWKDVKSGSKALDKYRLVNDYDAKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFP1</accession>
    <name>G3AFP1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetratricopeptide SHNi-TPR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_58161</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372442.1">
        <property type="nucleotide sequence ID" value="XM_007372380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFP1"/>
    <dbReference type="STRING" id="619300.G3AFP1"/>
    <dbReference type="GeneID" id="18872350"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58161"/>
    <dbReference type="eggNOG" id="KOG4563">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028900_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFP1"/>
    <dbReference type="OMA" id="IAECHYK"/>
    <dbReference type="OrthoDB" id="5587616at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051730">
        <property type="entry name" value="NASP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019544">
        <property type="entry name" value="Tetratricopeptide_SHNi-TPR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15081:SF1">
        <property type="entry name" value="NUCLEAR AUTOANTIGENIC SPERM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15081">
        <property type="entry name" value="NUCLEAR AUTOANTIGENIC SPERM PROTEIN NASP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10516">
        <property type="entry name" value="SHNi-TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Tetratricopeptide SHNi-TPR" evidence="4">
        <location>
            <begin position="193"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="103"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="345"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="103"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW35030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="381" mass="42766" checksum="D833D232AB12CEE0" modified="2011-11-16" version="1">MALYPENVSKLIAEGSRAYSAKDFDSAVAKYGEACETYAEEHGDDPDLLFLYGKAVFQSAISKSEVFGGAPPARTNDDNENGEGEQDEEDDQFQFYDAEPVEAEVTDEQEEEGPQLAEEDEEDEDEEDGKGKPEKQEEEESDFEIAWEILDLTRTLLEDKLSTLDKGDLSPPYLESDRSETTNEYIITVKKLADCYDILGDISLETENFQQSATDFQECLDLKLKLYNPENSGFLSEAYYKVALALEFCSDPQSRQKAAEQLKLAIESVQRRNKHETDSEMKKENDEMIQELQVKYDELRKAPSDQLQQEQMELIKGILGQEATSSGGDTSTSMSEVLVNNLTSMVKKKKPAQPVNDLSGMVKKRKAPKPDGNGVKKSKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFU9</accession>
    <name>G3AFU9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_64276</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372499.1">
        <property type="nucleotide sequence ID" value="XM_007372437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFU9"/>
    <dbReference type="GeneID" id="18874968"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64276"/>
    <dbReference type="eggNOG" id="ENOG502SBM6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097219_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFU9"/>
    <dbReference type="OMA" id="KCPIINW"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="296" mass="33722" checksum="E3DE776D9A0E8218" modified="2011-11-16" version="1">MAASLREVKLHTTAKRKLNELEDISNIHTTPKQVKNHHHKKQRVTFNTNEKPLTRHSLRQQQQQELKQHKITASVIIPQQQRSPSPSEGSSPPAAPEITRSKSVSFDLSNNSPLLTPKDEEEEEEEEYNVSEDVYSDEGSSSSVPSTPATPPGSTTSIDDHPEVEEKHSFASERNHEPTIGLDTNADYIALTSCLRLATNNRNKITQEIVELSQLQQYHSQNENKEETIEFFLKLMNNDLNLPKPNKTIKCPIINWGKYHPALTNVSKKFENCDQFKKEDLDDSVYKSLNLFKNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFZ4</accession>
    <name>G3AFZ4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA replication checkpoint mediator MRC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_48174</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372545.1">
        <property type="nucleotide sequence ID" value="XM_007372483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFZ4"/>
    <dbReference type="FunCoup" id="G3AFZ4">
        <property type="interactions" value="248"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFZ4"/>
    <dbReference type="GeneID" id="18871189"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48174"/>
    <dbReference type="eggNOG" id="ENOG502QSP5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_267364_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFZ4"/>
    <dbReference type="OMA" id="NPHEIRE"/>
    <dbReference type="OrthoDB" id="2130597at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024146">
        <property type="entry name" value="Claspin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018564">
        <property type="entry name" value="Repl_chkpnt_MRC1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14396">
        <property type="entry name" value="CLASPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14396:SF10">
        <property type="entry name" value="CLASPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09444">
        <property type="entry name" value="MRC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication checkpoint mediator MRC1" evidence="5">
        <location>
            <begin position="675"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="429"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="686"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="901"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="982"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="429"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="515"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1000"/>
            <end position="1015"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1015" mass="116387" checksum="5E830F2F98F6F739" modified="2011-11-16" version="1">MENTILFFLQPNPPFSFFAIDQQLIMDLLDQIEDYSSTQVINNLYQATQVINNNDSDSDSNSNHEDSDKENLNILDKINQRLNGNKIEESEEEEQEDEFQKLPQLEIDPTEFSQPTQVIQSSTQAITQVITHSDVIMDEPAILTEQQRQEKITKLIELKRQQRQQTEQTQLEKEIEAESSSDDDIINDTVVSTTDKDPSLKELNKIKQFLNIEKRKIDIQPEFERQVVFNKDKLLEAFSDDEEQEIIPAIESTPVTSPPEPSLTNITTTQIKNPLELYAQKLRKELPQEKAAIVLDSDSDTEVNTYTYTQHVPAQPIPELTKDQKLEIKQKFSKRKGAHAQTTHNSTSSLLKNLQARNISQLKQQKLNDPSKAIIEELDKDEEEMTSLLEREIQRAKNIRRKEKMQERAKLALVGKALGVAADEIDKDYEEGEELEESEVPESDYSGDEQGEDDSQAESDEEAQVKIAETRSDDSYMFGGQESDKQSDDDEPNITTTDRNDEDDDAADFNMNDSVLFQHSKPTQNVSSDEEDYAIVKRQVPSFKDLTQGSTQATQKDTTQGYIQVSTQPDATQVDITQKDPTQTQAITQPDKFAPTQKINLTQTIKTQVDDIFTDDEDEITPATVNKGKKAIRNNMIEQGEDEDEEDIQEQIKIFQEKIKKQELKQRKRRKEFERRGLNKIVEGEAVESEDEWQGIGGMDGEMSDQANSEDERMIDNNFNIDLNDEEVRKKFMEDYQIKDQKELEKLLDDIKNHRLNKRGINGNGFDIDLSDEEDQLLAAYRRQKLQEQQQRLLSNKKLVELEKNERSRPFFESIQESTSQIKIDDDDDGEEVAVDGKKTVDESFVQRQLSFLNKSHQDDDYVELQRISNIQYGYDDDDDVEDIQTLKTKSMNNLTRSVSTVLSSENNKRDSQQVTDADTDDDDEFMPAFKKPSIIKSFKSFQQENQTTSFGKSTFSGVTISKQYKSVSGLKASITYMQKNNASKKQTKSLKEKQIAKSLDNSRSSSLFKSTGFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG26</accession>
    <name>G3AG26_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="8">ATase</shortName>
            <ecNumber evidence="3 8">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_58367</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + L-glutamine + H2O</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="3 8"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372577.1">
        <property type="nucleotide sequence ID" value="XM_007372515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG26"/>
    <dbReference type="FunCoup" id="G3AG26">
        <property type="interactions" value="734"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG26"/>
    <dbReference type="MEROPS" id="C44.001"/>
    <dbReference type="GeneID" id="18872416"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58367"/>
    <dbReference type="eggNOG" id="KOG0572">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022389_2_1_1"/>
    <dbReference type="InParanoid" id="G3AG26"/>
    <dbReference type="OMA" id="IRHFGVK"/>
    <dbReference type="OrthoDB" id="191723at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00715">
        <property type="entry name" value="GPATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035584">
        <property type="entry name" value="PurF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11907">
        <property type="entry name" value="AMIDOPHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4 8 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="11">
        <location>
            <begin position="2"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="373"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW35165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="528" mass="58887" checksum="B6D66A2B664A4038" modified="2011-11-16" version="1">MCGILGVVVADQRKNVASELFEGAMFLQHRGQDAAGIVTSGPKGRFYQCKGNGMARDVFTQSRMNNLIGNMGICHLRYPTAGSSAGSEAQPFYVNSPYGITLSHNGNLVNSNELREYLDEVVHRHINTDSDSELLINIFAAELDKFNKSRVNNGDLFSALTGTMDMIRGAYACVAMLAGYGIIGFRDPNGIRPLLYGERTNKNGTTDYMLASESVVLKAHGFDKFRDIKPGEAVIITKDGAKPEFRQVMAPKIFAPDIFEYVYFARPDSVLDGVSVYRSRIDMGVKLAEKIQRSLSADEKIDVVIPVPDTARTSAFQCSVSLNVPYREGFVKNRYVGRTFIMPDQQERRSSVRRKLNAMDSEFQGRNVLLVDDSIVRGTTSKEIVAMAREAGAKKVFFASCAPPIRFNHIYGIDLADTKALVGFNRDEDEIASVIGADKVIYQDLEDLIDCCKRDEIQEFEVGVFNGKYVTGIEDNYIQELEKVRAQNQRMKEYRARGMSLDTNVDQEFVDVKPDVDISIHNSGDYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG62</accession>
    <name>G3AG62_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">KSP2</name>
        <name evidence="8" type="ORF">SPAPADRAFT_130712</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372613.1">
        <property type="nucleotide sequence ID" value="XM_007372551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG62"/>
    <dbReference type="FunCoup" id="G3AG62">
        <property type="interactions" value="532"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG62"/>
    <dbReference type="GeneID" id="18869520"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_130712"/>
    <dbReference type="eggNOG" id="KOG0583">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008377_1_0_1"/>
    <dbReference type="InParanoid" id="G3AG62"/>
    <dbReference type="OMA" id="TIDEEYW"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="17"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="57"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="330"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="490"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="668"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="739"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="330"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="352"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="506"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="560"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="581"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="740"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="827" mass="94100" checksum="142B021C41F7A3D4" modified="2011-11-16" version="1">MDEFEQYDKGQLLRNRYLKVGDISEGSYGLVSVAKDTHSNNKLVAVKFIYPVDYKKTRQEGQQQQQDHGSRPNSSPAKLRSPVVESKTPDGKHYRQSVINNLLNEAQKEIKIHKILGDHPNISKLYDHFDTCLVLEFCSRGDLYEAIHNGNGPATTQDIKDVFQQILNALEFCHSHGVYHRDLKPENILIAEDWSIKLCDWGLSTTTKVITNKDEFDIGSERYMAPELFDDNVESYDAAKIDIWSLGIILLTLVFHKNPFQVANYSDKRFIQFVNNREALFDIFSTMSGDLFSVLRFSLTIDPDNRNLASISNELKLLRYFTIDEEYWASDYEEEEEEEPEDIDDEYASYESDEKAHSESPTEKKLTQIEQFKLETEPLTPTVSITDTEGEPVKAILSRSPLSPRGGIEDIPHNHRADALLSSSTNLKPIPIGGSGYKFIRNTRKPLNVASYNQNSSRFQSSYNKFNREEYYTPKSVFNHYMDKYGEQKFGKLDNRSGSPFENGNKRPKHAKRTWKKNYKKKYKPNNNGGGGQQYQQNQHHPYHHQHTRSQDFANDSHNHIHPNRRKSRSYSTSKLKRPIFQSSGGMTGSPINNNLLSANHPTLTHPNVNVNGNSAPSATLSSSGKYVPPYLRSPNYQRSPVIEPLVEEMDHLSLNNDDDEVFHLEGDFESKSNGSGRQQPQHHHYNSGYGSDYSSHNRRNSRYGCDSGYNNFNSHHATNGTHPGRRNSVAAATRKAVFGNNNNVDNSQMLSTSATSTSSGKYIPPFKRGSVGTVPAVKRSPPDRKERPTSSTFSDMNKNPLIHNHVSLIGSEWISSYKKDWSDYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG77</accession>
    <name>G3AG77_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Inositolphosphotransferase Aur1/Ipt1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_58414</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372628.1">
        <property type="nucleotide sequence ID" value="XM_007372566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG77"/>
    <dbReference type="GeneID" id="18872444"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58414"/>
    <dbReference type="eggNOG" id="ENOG502QPKA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_921863_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG77"/>
    <dbReference type="OMA" id="CLGIITH"/>
    <dbReference type="OrthoDB" id="5784at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070916">
        <property type="term" value="C:inositol phosphoceramide synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006676">
        <property type="term" value="P:mannosyl diphosphorylinositol ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03386">
        <property type="entry name" value="PAP2_Aur1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026841">
        <property type="entry name" value="Aur1/Ipt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052185">
        <property type="entry name" value="IPC_Synthase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310:SF8">
        <property type="entry name" value="INOSITOLPHOSPHOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14378">
        <property type="entry name" value="PAP2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Inositolphosphotransferase Aur1/Ipt1" evidence="7">
        <location>
            <begin position="87"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="302" mass="34688" checksum="2446FE52F4C1D046" modified="2011-11-16" version="1">MDTSEMILTSPSLASDTPSIVSPPSESEILESGLFEFTEESASTIDIPKSKAHHHWRLAPVFLLAISWTVLNILYSTKNPVTKPKNIVAWVFHVVVHFVVPPLFGGWLYIFHPPRALKLFIVSSGLQTVAITLTYLVFPNAPPLFIKLYGENKEPTFDMIYTDGITSVDMRFGVMLHKLSYYAIPNKFSSFPSLHSANACLIFFFVCYYSKWTSFKLLGLVNVLGQWWSELYLDHHWRFDCFAGMLYAITTWVLLMNWKKGLQYVDDRYTNAKANLDMKHGSTMGMRLFRNTRLKNFFDPQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGD0</accession>
    <name>G3AGD0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">rRNA-processing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_58485</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Involved in nucleolar integrity and required for processing of the pre-rRNA for the 60S ribosome subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Nucleus</location>
            <location evidence="2 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the CGR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372681.1">
        <property type="nucleotide sequence ID" value="XM_007372619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGD0"/>
    <dbReference type="FunCoup" id="G3AGD0">
        <property type="interactions" value="156"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGD0"/>
    <dbReference type="GeneID" id="18872468"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58485"/>
    <dbReference type="eggNOG" id="ENOG502S7VB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125051_0_1_1"/>
    <dbReference type="InParanoid" id="G3AGD0"/>
    <dbReference type="OMA" id="NGKQWHD"/>
    <dbReference type="OrthoDB" id="3942380at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005579">
        <property type="entry name" value="Cgr1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03879">
        <property type="entry name" value="Cgr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 8" id="KW-0698">rRNA processing</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="59"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="132" mass="15822" checksum="9F42044DFDD1D806" modified="2011-11-16" version="1">MSSTANLQYKDIPKKYIDPLAPKDKGDGSRVNGKNWKISKDAFRVKTLGVTKPNSFQARNEKKLQEEQYKARLKELKEEKENIKKQRIDDLKRRREIKAEKERYEKLAAKMHAKRVERLRKKEKRNKLLKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGD4</accession>
    <name>G3AGD4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cohesin loading factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_130860</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SCC4/mau-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372685.1">
        <property type="nucleotide sequence ID" value="XM_007372623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGD4"/>
    <dbReference type="STRING" id="619300.G3AGD4"/>
    <dbReference type="GeneID" id="18869535"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_130860"/>
    <dbReference type="eggNOG" id="ENOG502RK5N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017317_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGD4"/>
    <dbReference type="OMA" id="AAKYYYL"/>
    <dbReference type="OrthoDB" id="5565328at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019440">
        <property type="entry name" value="MAU2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10345">
        <property type="entry name" value="Cohesin_load"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="692"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="693"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="711" mass="82051" checksum="20A5D973F3CDE041" modified="2011-11-16" version="1">MISYYIGQSDRFVRLAYQSSTITGLENHNKFIILAIKMLLIVTKKYKAKLKPKQESIVYYRLGKLYLIETTNIHKAEEYINKALDIANFHEFKQIRFYCELLLAQILETTNLKLCANYVFSKGQYYKQKGDHVYSNLFQLLRIKSLIVSDCATALVVLQGSILDPNLNDSIKCLCLIYEANLHCYRGSPDIAIKLLGEAEQLAYRDGDNPIMPVQIDGMILLCRLTAYSNMNMVAEAESCRKLISNYIKAQTEEGWKSWNDNGIVRVEIDELDHDTTIPFLFRWLNSDEFVITYYLITGSTYMSHDGYSEKVFDRCLQMISDQKKRISNGGSKSLLSPREVANRMLKLRYLQFVIAFYKTWIGFLKGEYKKITNMNVFMNENNSKFSSEEYSCFKPLLPLIYYMFAAFYQDKGDIQAAKYYYLKVRTMTSSNMSQETKKSPEQIINTSLFQLQLGIGCDALAAKGQFNELYVFSSIQLYILLEFQLTEIKQNVSNVKDQQMLEDHVNLKSELVQELRNIFKAKQTSNSFAVNFTGSNLMLTITYFLVNDILSETQEGDKSGIEPSLEASFHTGLKLLGKKTTFTLMHFLISNYILTKSSNMDQKERISEMVKEQVAQNGLKSPQSTPDLSKIIRVMLLKHLQKKYHSVGDLQNAKMAELQSSYYYKCLQDKFKFLFENTRYFDKGDIPVAKINSESPPTTSDSVERKQLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGJ5</accession>
    <name>G3AGJ5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">6,7-dimethyl-8-ribityllumazine synthase</fullName>
            <shortName evidence="9">DMRL synthase</shortName>
            <ecNumber evidence="4 9">2.5.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_146462</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="2.5.1.78"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homopentamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the DMRL synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.78" evidence="4 9"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372746.1">
        <property type="nucleotide sequence ID" value="XM_007372684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGJ5"/>
    <dbReference type="FunCoup" id="G3AGJ5">
        <property type="interactions" value="303"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGJ5"/>
    <dbReference type="GeneID" id="18870719"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_146462"/>
    <dbReference type="eggNOG" id="KOG3243">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089358_2_2_1"/>
    <dbReference type="InParanoid" id="G3AGJ5"/>
    <dbReference type="OMA" id="CQGVTQG"/>
    <dbReference type="OrthoDB" id="2965at2759"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00404"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000906">
        <property type="term" value="F:6,7-dimethyl-8-ribityllumazine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09209">
        <property type="entry name" value="Lumazine_synthase-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.960:FF:000007">
        <property type="entry name" value="6,7-dimethyl-8-ribityllumazine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="entry name" value="Lumazine/riboflavin synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00178">
        <property type="entry name" value="Lumazine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034964">
        <property type="entry name" value="LS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00114">
        <property type="entry name" value="lumazine-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058:SF0">
        <property type="entry name" value="6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058">
        <property type="entry name" value="6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE DMRL SYNTHASE LUMAZINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="Lumazine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="164" mass="18072" checksum="F3AA3B3AEB04A559" modified="2011-11-16" version="1">MAIGGLGQLDQEYDGSNLRIGILHARWNKEIIDALVEGAIKKLLSLNVKRENIIIETVPGSFELPYGSKLFVEKQKRLGKPLDAVIPIGVLIKGSTMHFEYICDSTTHQLMKLNFELGIPVIFGVLTCLTLEQAEARAGLIEGKMHNHGEDWGAAAVEMATKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGK8</accession>
    <name>G3AGK8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58574</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372759.1">
        <property type="nucleotide sequence ID" value="XM_007372697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGK8"/>
    <dbReference type="STRING" id="619300.G3AGK8"/>
    <dbReference type="GeneID" id="18872510"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58574"/>
    <dbReference type="HOGENOM" id="CLU_1262211_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGK8"/>
    <dbReference type="OrthoDB" id="4096268at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="219" mass="25660" checksum="1A9757FEFC0003D3" modified="2011-11-16" version="1">MNWMNWKRVPIWKYQKTIMKQKSSKKKKKKMMMMVRMRMVGILEIFDDDAEEEEEEDDDEDDDDDDDEEEEEVYEYDLLEQDDQGDEDEYDLLEEEDSESEDNGVDDESVVLQNIQICLTQDMIKCDQDSENSESNVTSCNIPTVSYTPSCHTRKRNHQEMEENKDKEPVVEEPPTKKKRSSVIKTIAKEIGKGLFYAIATITALGIYGHSLERQDQSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGM5</accession>
    <name>G3AGM5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58588</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372776.1">
        <property type="nucleotide sequence ID" value="XM_007372714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGM5"/>
    <dbReference type="GeneID" id="18872518"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58588"/>
    <dbReference type="eggNOG" id="ENOG502TF7M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1323704_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGM5"/>
    <dbReference type="OrthoDB" id="189997at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442291" evidence="1">
        <location>
            <begin position="23"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="208" mass="24344" checksum="49CFCB5DE1099939" modified="2011-11-16" version="1" fragment="single">FDIENLKLLLLLTIHAITSVNRDLCWNLIGVLNRAVIKWDMYTSKDLLQQRIVWTIHNLDKEISLLLKKPSQFPSYEYLQVEYPLTIKLYEEESLPLVNKNIELSKLKQTILHLNLTQSKSDLHELSGELEKWRISSSKVIHQSSDISDLISWINMQYYYLLIELDQVSDSQSFQFTLQYLSNSFTLLISSSDRQKSNTKLSITSNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGT4</accession>
    <name>G3AGT4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="12">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Tyrosyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TYS1</name>
        <name evidence="14" type="ORF">SPAPADRAFT_58633</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="12"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372829.1">
        <property type="nucleotide sequence ID" value="XM_007372767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGT4"/>
    <dbReference type="FunCoup" id="G3AGT4">
        <property type="interactions" value="816"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGT4"/>
    <dbReference type="GeneID" id="18872545"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58633"/>
    <dbReference type="eggNOG" id="KOG2144">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035267_0_1_1"/>
    <dbReference type="InParanoid" id="G3AGT4"/>
    <dbReference type="OMA" id="RKIHMLA"/>
    <dbReference type="OrthoDB" id="197206at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000004">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000040">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023617">
        <property type="entry name" value="Tyr-tRNA-ligase_arc/euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050489">
        <property type="entry name" value="Tyr-tRNA_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006330">
        <property type="entry name" value="PRK08560.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264:SF4">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264">
        <property type="entry name" value="TYROSINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006588">
        <property type="entry name" value="TyrRS_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12 14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="356"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="368"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW35417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="412" mass="44875" checksum="1A68AC9CDD7F6E44" modified="2011-11-16" version="1">MSSTFDNVVTDPTEQYNLITKGLQETLNGQIIKDILEKEKRHVKIYWGTAPTGKPHCGYFVPMIKLAHFLKAGCEVTVLLADLHAYLDNMKAPLEVVQHRSKYYELVVKAILRSIGVPIEKLKFVVGSSYQQGGDYVLDLFKLSNIVSQNDAKRAGADVVKQVANPLLSGLIYPLMQAIDEQHLDCDAQFGGVDQRKIFVLAEENLPSIGYKKRAHLMNPMIPGLGQGGKMSASDPNSKIDIIEDPKVVKKKINSAYCAPGEVKDNGLIAFIEYVVQPIQELKAGQDGVFDFKIDRPEKYGGPITYTSLDALKADYESGALAPPDLKSGTADRINELLAPIRAEFESNAEFQAAQKNGYPAAEAKQEKKVKKPKNKGTRYPGGAAAAAAAAPADANGAVEKTAEALETVKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGT6</accession>
    <name>G3AGT6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_58637</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372831.1">
        <property type="nucleotide sequence ID" value="XM_007372769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGT6"/>
    <dbReference type="FunCoup" id="G3AGT6">
        <property type="interactions" value="125"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGT6"/>
    <dbReference type="GeneID" id="18872547"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58637"/>
    <dbReference type="eggNOG" id="KOG0724">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019916_1_0_1"/>
    <dbReference type="InParanoid" id="G3AGT6"/>
    <dbReference type="OMA" id="RNHTWSE"/>
    <dbReference type="OrthoDB" id="1690618at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101031">
        <property type="term" value="C:protein folding chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051083">
        <property type="term" value="P:'de novo' cotranslational protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006452">
        <property type="term" value="P:translational frameshifting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23953">
        <property type="entry name" value="zuotin_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.840">
        <property type="entry name" value="Ribosome-associated complex head domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032003">
        <property type="entry name" value="RAC_head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042569">
        <property type="entry name" value="RAC_head_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054076">
        <property type="entry name" value="ZUO1-like_ZHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044634">
        <property type="entry name" value="Zuotin/DnaJC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058871">
        <property type="entry name" value="Zuotin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43999">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43999:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16717">
        <property type="entry name" value="RAC_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21884">
        <property type="entry name" value="ZUO1-like_ZHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26185">
        <property type="entry name" value="Zuotin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="5">
        <location>
            <begin position="91"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="292"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="63"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="292"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="342"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="428" mass="48373" checksum="E743660CF68F833B" modified="2011-11-16" version="1">MSIVLPSGSGDSFKAVNKYSAAVRRPIEPVGRYFLAHASRTLRGHTWSEFEKLEAERNVKQVEEDEDDDLGDEEQSEELLAHDPREWKTANLYAVLGLSHLRWKATEDQIRRAHRKQVLKHHPDKKSAAGGLDQDGFFKIIQKAFEIMLDPIKRRQYDSVDTDNDPKPPSAKTKYDFFEVWGPIFESEGRFSVKQPVPALGGLDATKEQVDEFYGFMNKFESWKTFEFKDEDVPDDTANRDHKRYIERKNIATRKKLKQEDNKRFIELVERAYSEDPRIKMFKDQAKKEKAAKKWEREAGSRKAAEEEAAKKAAEEEAAKKAAEAEASKKADSKKAKEAAKAAKKKNKRSVRNSAKDNDYFGDAGKASDIDADIELLLEKFDDVKLGEVAAKVKDADAAGVKAALSEVVAELVGAGSLQASYLKYFSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGX5</accession>
    <name>G3AGX5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphoglycerate mutase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_130940</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372060.1">
        <property type="nucleotide sequence ID" value="XM_007371998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGX5"/>
    <dbReference type="FunCoup" id="G3AGX5">
        <property type="interactions" value="154"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGX5"/>
    <dbReference type="GeneID" id="18869540"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_130940"/>
    <dbReference type="eggNOG" id="KOG0235">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_9_2_1"/>
    <dbReference type="InParanoid" id="G3AGX5"/>
    <dbReference type="OMA" id="NYPNMET"/>
    <dbReference type="OrthoDB" id="354304at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045820">
        <property type="term" value="P:negative regulation of glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043456">
        <property type="term" value="P:regulation of pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000051">
        <property type="entry name" value="Phosphoglycerate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051695">
        <property type="entry name" value="Phosphoglycerate_Mutase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46517">
        <property type="entry name" value="FRUCTOSE-2,6-BISPHOSPHATASE TIGAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46517:SF1">
        <property type="entry name" value="FRUCTOSE-2,6-BISPHOSPHATASE TIGAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="7">
        <location>
            <position position="21"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="20"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="25582" checksum="66C21C3DA9F5CC22" modified="2011-11-16" version="1">MTREITDNKDSDITRVFIVRHGQTDHNVNKILQGHLDTDLNELGKQQAVTVGKYLSQLPIDYFVSSDLTRCRETIKEVLAYQNNKDNVKYTSNLRERDMGHCEGMYLKDALAQFGPAFRNLGEKEEELCARVETEWNTISKLNHKNVLVCTHGGVITRFTNFLYTNMQYELDESITPHDLKVPFNTSITVIDINKKTNTGKIQQFGNTVHLGGNFEVKDQLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGY5</accession>
    <name>G3AGY5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_57709</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372070.1">
        <property type="nucleotide sequence ID" value="XM_007372008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGY5"/>
    <dbReference type="STRING" id="619300.G3AGY5"/>
    <dbReference type="GeneID" id="18872162"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57709"/>
    <dbReference type="eggNOG" id="ENOG502S162">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048444_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGY5"/>
    <dbReference type="OMA" id="HNRTRTY"/>
    <dbReference type="OrthoDB" id="2152248at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050261">
        <property type="entry name" value="FrsA_esterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22946:SF0">
        <property type="entry name" value="DIENELACTONE HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22946">
        <property type="entry name" value="DIENELACTONE HYDROLASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="330" mass="38307" checksum="50AEB4947E5008D7" modified="2011-11-16" version="1">MQIFEFDPKSFSKEKYNVGGISTYVYNSDLLVPYIKQFDDANLPHIEEIPINVLYLVHQRGGDHRFTESIAYNVLKQFYEKRPASDDTTPLICVTFDIRNHGERLVDELRNSGWTKGNQSHAVDMVSGIEGNVRDLKLIIDYLPSYLNLNHFLGAQFRERNPSCEIKFRNLLSGYSLGGHTVFRFASEYPELVKIINPVVGCHDLTSLLVNRLKQTPLDSPSYHKKYFYFHYDELDLNTEQKQLNYPEVFHKRLSYEDTRIFENFPMNKIKMFAAFGKEDTLVPVKLSSVWCDVYVNSNSACEVFVQEGVGHDVTPEMIDRFTTWLVKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH16</accession>
    <name>G3AH16_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_58681</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peroxiredoxin family. Prx5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372858.1">
        <property type="nucleotide sequence ID" value="XM_007372796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH16"/>
    <dbReference type="FunCoup" id="G3AH16">
        <property type="interactions" value="582"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AH16"/>
    <dbReference type="GeneID" id="18872562"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58681"/>
    <dbReference type="eggNOG" id="KOG0541">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072440_1_1_1"/>
    <dbReference type="InParanoid" id="G3AH16"/>
    <dbReference type="OMA" id="HVPEYIQ"/>
    <dbReference type="OrthoDB" id="195498at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03013">
        <property type="entry name" value="PRX5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037944">
        <property type="entry name" value="PRX5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430">
        <property type="entry name" value="PEROXIREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430:SF16">
        <property type="entry name" value="PEROXIREDOXIN-5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 7" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5 7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="4"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="6">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR637944-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="173" mass="19007" checksum="B8708BC8360C8ED4" modified="2011-11-16" version="1">MSGKFPTNINPKYIPYTKLHDGVLSCDVPVELNLANTFKGKKVVVTAVPGAFTPTCTEQHIPDYLKNLAEFKKKGIDKIVVLSANDPFVMAAWGKALGYRDEENYIVFATDPNATISKQLGDNYVADLSAVGFGLRLQRYAALVEDGEIKYLGNEDELGFTEISSAKNLLDQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH17</accession>
    <name>G3AH17_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SUN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_48439</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372859.1">
        <property type="nucleotide sequence ID" value="XM_007372797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH17"/>
    <dbReference type="STRING" id="619300.G3AH17"/>
    <dbReference type="GeneID" id="18871218"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48439"/>
    <dbReference type="eggNOG" id="ENOG502SGS1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_416173_0_0_1"/>
    <dbReference type="InParanoid" id="G3AH17"/>
    <dbReference type="OMA" id="YNANHVL"/>
    <dbReference type="OrthoDB" id="4093311at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="182"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="218"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="8"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="162"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="647" mass="75202" checksum="CB4D3B160E8165F0" modified="2011-11-16" version="1">MPSATAYIQQQKQKIQQGQMQHRIQANHSRQNNIDNKIIDTIHSSDDIDLDELNLDEEDVNVSYEKYLTAEHSDEFEQQQALNDANYNEDEDEDYTDSDTYKESDDDIEADFDDVEEPELYTDDFHQKSVFNEDTQYYDPKQPSPPRSPIFDQIQQEQNQFPRKKPVQPKNKPKKPSNNNSWNILTIAGTVGLVSFLVTVIAYYVLNNNQIPVPEFSMRKINAKFQYFDEKLNQLESETRQSNRLLDNGINKLSKQIHSLEQNIQKSPVSYKNNQIQITPELHQFLFKFIENYSQKQTSEELTRYIDEKLKSFSITEIESMIQDMKNFQSGQYEAILSQIMSKLPKTQDTPSEELKAYIDSQVTQALDHLNEEIHSRLSYILDNLTIVNNTIIPDTQIHPSNEIWLNSMLDLFSRGSIKVNYADYSLGARILGFLTSYPKNKTHSIWYKMAYGWWIYSENKHDQHNANHVLMDDDATWICDNSECQLGLRLYSAIIPTDLLIELEGADTHTVELGFKPNSRTGYDKLSQFDGMSSKTNPYLKKFKLIKSIKLSSGLVHVRFPIRFVNLQVSGRDVYLKFTPNSKNPIHVKRVKVYGINEVDAVKYADQFKLMIGQFTQAQDVESAVEDTRKKNDRIGHYDLDKDIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH20</accession>
    <name>G3AH20_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Inhibitor I9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_58684</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Cytosolic inhibitor of vacuolar proteinase B (yscB), probably regulating protease B activity during limited proteolysis. PBI2 is a component of the LMA1 complex, which is involved in the facilitation of vesicle fusion such as homotypic vacuole and ER-derived COPII vesicle fusion with the Golgi.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the heterodimeric LMA1 complex together with the thioredoxin II/TRX2. LMA1 binds to the ATPase SEC18.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protease inhibitor I9 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372862.1">
        <property type="nucleotide sequence ID" value="XM_007372800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH20"/>
    <dbReference type="FunCoup" id="G3AH20">
        <property type="interactions" value="172"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AH20"/>
    <dbReference type="GeneID" id="18872563"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58684"/>
    <dbReference type="eggNOG" id="ENOG502SBW1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156026_3_0_1"/>
    <dbReference type="InParanoid" id="G3AH20"/>
    <dbReference type="OMA" id="IITCKPD"/>
    <dbReference type="OrthoDB" id="5518345at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000005">
        <property type="entry name" value="Proteinase inhibitor I2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052471">
        <property type="entry name" value="PBI_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28288">
        <property type="entry name" value="PROTEASE B INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28288:SF2">
        <property type="entry name" value="PROTEASE B INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0722">Serine protease inhibitor</keyword>
    <feature type="domain" description="Inhibitor I9" evidence="6">
        <location>
            <begin position="5"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00062658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="72" mass="7933" checksum="BBBE319640B63211" modified="2011-11-16" version="1">MSLKSYIITLKEDVSDVDFNSVKSLIGDLGGEITNEFTLIKGFAAKLPGEVSALTKDDRILHVEEDKEVHIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH32</accession>
    <name>G3AH32_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Inositol-pentakisphosphate 2-kinase</fullName>
            <ecNumber evidence="4 10">2.7.1.158</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_131921</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Has kinase activity and phosphorylates inositol-1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5) to produce 1,2,3,4,5,6-hexakisphosphate (InsP6), also known as phytate.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ATP = 1D-myo-inositol hexakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20313"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.158"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="10">The EXKPK motif is conserved in inositol-pentakisphosphate 2-kinases of both family 1 and 2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the IPK1 type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.158" evidence="4 10"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372874.1">
        <property type="nucleotide sequence ID" value="XM_007372812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH32"/>
    <dbReference type="FunCoup" id="G3AH32">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AH32"/>
    <dbReference type="GeneID" id="18869621"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131921"/>
    <dbReference type="eggNOG" id="ENOG502S7VH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046294_1_0_1"/>
    <dbReference type="InParanoid" id="G3AH32"/>
    <dbReference type="OMA" id="FIELRCK"/>
    <dbReference type="OrthoDB" id="272370at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035299">
        <property type="term" value="F:inositol-1,3,4,5,6-pentakisphosphate 2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.110">
        <property type="entry name" value="Inositol-pentakisphosphate 2-kinase, N-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009286">
        <property type="entry name" value="Ins_P5_2-kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043001">
        <property type="entry name" value="IP5_2-K_N_lobe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14456">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14456:SF2">
        <property type="entry name" value="INOSITOL-PENTAKISPHOSPHATE 2-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06090">
        <property type="entry name" value="Ins_P5_2-kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW35462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="307" mass="35505" checksum="07C733D68E60533A" modified="2011-11-16" version="1">MELDKISTPIEWKFVAKGGANVLFKYTGNNELLRSKLLRLRISKPESIPTKQLYEFTESKCKPIFQDKLIESEIITVPTEFISKLDSPVDIIEPWGLLMPNMLDGTFSTLDLSKWCQLHCDLGSAKVILELKPKWLYDCKTNYCRTCSLSQSRGHARHFCPLDLVYDPDSATNDLFKKVPHDTLSAIESTIPVRKLFKEYLEDPDNIFQQLKTLQEIANEEDLIENLTCADDVSDRLSFIMTLRDVGVFIKFQQVGNNFSTTCKVYDLDLKSNDKCSHWVKIEQRLKDYYNSTNENWRHCTKSNHLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH80</accession>
    <name>G3AH80_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_132292</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S9 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372923.1">
        <property type="nucleotide sequence ID" value="XM_007372861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH80"/>
    <dbReference type="FunCoup" id="G3AH80">
        <property type="interactions" value="1193"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AH80"/>
    <dbReference type="GeneID" id="18869650"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132292"/>
    <dbReference type="eggNOG" id="KOG1463">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029573_2_1_1"/>
    <dbReference type="InParanoid" id="G3AH80"/>
    <dbReference type="OMA" id="ESKIYHA"/>
    <dbReference type="OrthoDB" id="3098at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000010">
        <property type="entry name" value="26S proteasome regulatory subunit RPN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050871">
        <property type="entry name" value="26S_Proteasome/COP9_Components"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040780">
        <property type="entry name" value="Rpn6_C_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040773">
        <property type="entry name" value="Rpn6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10678">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 11/COP9 SIGNALOSOME COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18503">
        <property type="entry name" value="RPN6_C_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18055">
        <property type="entry name" value="RPN6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="223"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="421" mass="47922" checksum="94D7EC8178A9A8DD" modified="2011-11-16" version="1">MSEQLLKEAKEATTAKQYDVAEAKYTQLIQDTTTTTNPPAKVLQQQETAILELGKIYEAQQDANKLADLITHSRQVLGNFAKSKTAKIVKTLIENFDKIEDSLDLQIKATRESIEWAVTSKLSFLRQSLQLKLSDLLYQKHNYQEALKNITDLLREYKKLDDKSSLVEVQLLESKIYHALRNIPKSRAALTSARTSANSIYCPTILQAELDCQSGILNAEDKDYKTAFSYFYESFEGFNSQEDPRSIVVLKYMLLTKIMLNLVDDVNNILNNKNVIKYKSRDIDAMKAIALAYSNRSLKEFETSLLTYTDELKSDPIIKNHFNALYDNLLEQNLLKIIESYSCVELSHISKTIGLNLQQVEGKLSQMILDKVFYGVLDQGNGWLIVYDEPRKDASYEASLDLIKNLSSVVDLLYEKASSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH92</accession>
    <name>G3AH92_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_48509</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372934.1">
        <property type="nucleotide sequence ID" value="XM_007372872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH92"/>
    <dbReference type="GeneID" id="18871232"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48509"/>
    <dbReference type="HOGENOM" id="CLU_733972_0_0_1"/>
    <dbReference type="InParanoid" id="G3AH92"/>
    <dbReference type="OrthoDB" id="10607671at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="279"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="116"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="93"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="377" mass="44039" checksum="9DD1173794894B45" modified="2011-11-16" version="1">MIKDEDIKQAKTQNNILREKLKAALEQEKKKKQEQQQQQSQRFDSDDTLDQLINLDLTNHTSKEERWLELKQRMNRINSQDYTLPQTTPPRTTPTSSTISSSNSIKTTDHNLLDQINHLNQTISSLNQQLKQQQTERNDLIEEFQLKETKMISIHNKENKELKHKYNRELESVKEQLVASNEKVKILTADNQQYKYNAHKLESKLTEAKTKFDKLNSKYEKLKKIYEQQQVDVHERTREQPSMAYVDPLRNLQSEHPRVHADQSKFYSDHHPRIYSEQARLHSDQPRVYSDQPKDVNSDTTNGLFSLKPTGQVIDNDSDSDDELLERKLSPISPITQPLDSGTTSTQVLLNQSSNNIGNRGNYHPEDDTNYLLNLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHB2</accession>
    <name>G3AHB2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Mitochondrial chaperone BCS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_59502</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family. BCS1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373660.1">
        <property type="nucleotide sequence ID" value="XM_007373598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHB2"/>
    <dbReference type="FunCoup" id="G3AHB2">
        <property type="interactions" value="1127"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHB2"/>
    <dbReference type="GeneID" id="18872946"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59502"/>
    <dbReference type="eggNOG" id="KOG0743">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010189_6_0_1"/>
    <dbReference type="InParanoid" id="G3AHB2"/>
    <dbReference type="OMA" id="WMTLYQR"/>
    <dbReference type="OrthoDB" id="10251412at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19510">
        <property type="entry name" value="RecA-like_BCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000768">
        <property type="entry name" value="Probable mitochondrial chaperone bcs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014851">
        <property type="entry name" value="BCS1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050747">
        <property type="entry name" value="Mitochondrial_chaperone_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23070">
        <property type="entry name" value="BCS1 AAA-TYPE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08740">
        <property type="entry name" value="BCS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01024">
        <property type="entry name" value="BCS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="BCS1 N-terminal" evidence="13">
        <location>
            <begin position="46"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="246"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="40592" checksum="960F9B66FE33CDA6" modified="2011-11-16" version="1" fragment="single">MAQPPQSIDFDKIRNSSDGTFKGLVSSGFNEIVGNNPYFAAGGGLMVLGTVLAVARQGVMKSSGFIYRQLLVDLEIPSKDKSYLWFLEWMSKYKHRSSRHLSVETSYVQHDNGSVSTKFSLVPGPGKHLLRYKGAFMLVNRERSGKLIDMTSGTPFETVTLTTLYRDRKLFGDMLLEAKQLALKAREGKTVIYTSWGPEWRPFGQPKTKRLFESVILDEGIGESILKDVRDFLNSGEWYRKRGIPYRRGYLLFGPPGSGKTSFIQALAGELDYNICILNLSENNLTDDRLNHLMNHIPDRSILLLEDIDAAFNKRDQTDEKGFNNGVTFSGLLNALDGVASAEECITFMTTNHPEKLDPALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHB6</accession>
    <name>G3AHB6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_65259</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373664.1">
        <property type="nucleotide sequence ID" value="XM_007373602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHB6"/>
    <dbReference type="GeneID" id="18875031"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65259"/>
    <dbReference type="HOGENOM" id="CLU_998078_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHB6"/>
    <dbReference type="OrthoDB" id="425749at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="279" mass="32013" checksum="59C8CF19C78D3DC4" modified="2011-11-16" version="1">MTPNELGYLISSIKDKDAEFFETDKVSLKGYLNFQDWRDNFIAKCNQVGLHIDESLLFDDDVDQGLRDEATSVVDEFVQTGIEKTVPVKWRAVLDQDKKGIDNYHQIVDKYGIEINLESAVDILGEFLTRPIPGDEASAFWNNIVSKFDIKELGGLLYLANIDKDKRQQILNKHREHNQQFSIEAVEEYCKELNIELPKSVKFTMTNLLTIDTKLSKNSSSQLTPPDSPTKRTRSHQLTPRRNNGRRSKCRYCGALGHYIDECRKLKAAEEKRKQAQVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHB8</accession>
    <name>G3AHB8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcription elongation factor 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_135141</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Transcription elongation factor implicated in the maintenance of proper chromatin structure in actively transcribed regions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ELOF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373666.1">
        <property type="nucleotide sequence ID" value="XM_007373604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHB8"/>
    <dbReference type="FunCoup" id="G3AHB8">
        <property type="interactions" value="657"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHB8"/>
    <dbReference type="GeneID" id="18869871"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135141"/>
    <dbReference type="eggNOG" id="KOG3214">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105983_0_3_1"/>
    <dbReference type="InParanoid" id="G3AHB8"/>
    <dbReference type="OMA" id="CLDANKK"/>
    <dbReference type="OrthoDB" id="445983at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.190:FF:000001">
        <property type="entry name" value="Transcription elongation factor 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007808">
        <property type="entry name" value="Elf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038567">
        <property type="entry name" value="T_Elf1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934:SF0">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05129">
        <property type="entry name" value="Zn_ribbon_Elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="84"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="92"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="109"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW34082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="123" mass="13812" checksum="7712F888DECA1A8C" modified="2011-11-16" version="1">MGKRKSSAKKPVKKIKQTLDTQFTCLFCNHEKSVICTIDKKNLLGELHCKICGQSFQTAIHSLSQPVDIYSDWIDACEDLAEEIDQQGGEPGEGEAGEGDDVYSDDEYDTKAKIKRMDSEDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHF1</accession>
    <name>G3AHF1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N-acetyltransferase HPA3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_54305</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373699.1">
        <property type="nucleotide sequence ID" value="XM_007373637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHF1"/>
    <dbReference type="FunCoup" id="G3AHF1">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHF1"/>
    <dbReference type="GeneID" id="18871849"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54305"/>
    <dbReference type="eggNOG" id="KOG3216">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_32_1_1"/>
    <dbReference type="InParanoid" id="G3AHF1"/>
    <dbReference type="OMA" id="VHFLYHR"/>
    <dbReference type="OrthoDB" id="7305308at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051016">
        <property type="entry name" value="Diverse_Substrate_AcTransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10545">
        <property type="entry name" value="DIAMINE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10545:SF29">
        <property type="entry name" value="GH14572P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="3"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="154" mass="18094" checksum="54A0FC44D48371BC" modified="2011-11-16" version="1">MSVSLRPIKDEDKERWLELWTGPGGYIEFYKSLHKLTPEISNETFRRFLDPNEPVYSVVAIDDSTGTIIGFANYLTHRNTWTIENALYLNDLFVCSDNRLHGVGRKLIEYIYSEADRLNCKKCYWSTQFENHRAQLLYTKVGVKSGFLLYRRPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHG5</accession>
    <name>G3AHG5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_59547</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373713.1">
        <property type="nucleotide sequence ID" value="XM_007373651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHG5"/>
    <dbReference type="GeneID" id="18872967"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59547"/>
    <dbReference type="HOGENOM" id="CLU_870345_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHG5"/>
    <dbReference type="OrthoDB" id="10530893at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="320" mass="38563" checksum="333A8E0FCCF1459C" modified="2011-11-16" version="1" fragment="single">MESRVYLMYLMNCEPPSVITGILFHFVKVESDEQLLYDLIHRAEVEYAPEYVIKYQDHYTWNSKYDRNKTSIGDLSDIFCDFDESETKVDSGYLKRTFEFKLTLVPFFVPNDSKIKLPPGVDPSWFEIVPVHNDTHLIKHLLTTVRGDQQNYLQLTRTGKLIYRDLKKSFADCQVFPCDSRSINKIIDIIVEKMILCFKLLNERYSYYNACRTNPLAKRIFERMDQEQLHKYMPFPVWRDQKLITVFHVSDYWLFRKLKKERNITIDESFEIYQTRKHTKYDRCHHGHKEISWYYQNSYTLLFRNGKLELRKKFPRGYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHK9</accession>
    <name>G3AHK9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ACF4</name>
        <name evidence="11" type="ORF">SPAPADRAFT_59609</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 81 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373757.1">
        <property type="nucleotide sequence ID" value="XM_007373695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHK9"/>
    <dbReference type="GeneID" id="18872984"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59609"/>
    <dbReference type="eggNOG" id="KOG2254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005482_2_1_1"/>
    <dbReference type="InParanoid" id="G3AHK9"/>
    <dbReference type="OMA" id="FALKYKW"/>
    <dbReference type="OrthoDB" id="4473401at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052861">
        <property type="term" value="F:endo-1,3(4)-beta-glucanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1170:FF:000001">
        <property type="entry name" value="Endo-1,3-beta-glucanase Engl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1170">
        <property type="entry name" value="glycoside hydrolase family 81 endo-[beta] glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005200">
        <property type="entry name" value="Endo-beta-glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040720">
        <property type="entry name" value="GH81_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040451">
        <property type="entry name" value="GH81_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983">
        <property type="entry name" value="ENDO-1,3(4)-BETA-GLUCANASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983:SF20">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17652">
        <property type="entry name" value="Glyco_hydro81C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03639">
        <property type="entry name" value="Glyco_hydro_81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52008">
        <property type="entry name" value="GH81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 81 N-terminal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 81 C-terminal" evidence="10">
        <location>
            <begin position="210"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="575" mass="64147" checksum="00574F31C9BFC243" modified="2011-11-16" version="1">MGFITSVYYGNLVPRINSNVGFKTLAQEQSTKLTNANLLRYRATLFNGVQYLIYVKLPSGKKTTDFSFKVTNQNTITGSKAINGLIVQYAIAATAAHNTYYDEAAGMYVTECKVKAHGYGGTSAEYVLAYSKSGSSISKMPMVFALPHIVESVDSNTAQYNTGISLTSTNKGEMKGFRTDTLRMVENLNTNIQFLPWVQEMGTKAPSWTAAQLKLISQVANKELSVDINKLVNSQNSNYFSGKVLDKYAYILYVVSDIIGDAKLTKSLLATLKTTFATFRNNKQFYPLMYDTKFGGITSTAYKVSNDPNADYGSSYYNDHHFHYGYFIHAAAIIGYVDKKAGGTWAKDQQPWVNALIRDAANPSPGDPYFPVFRMFDWFQGHSWASGLFEGGDGRNEESSSEDYNFSYAIKLWGKVTGNKRMESYGDLMLAVMKRSMNKYFLYSSNNNVEPSNFIGNKVSGILFENKIAYTTYFGNPDTNPEYVHGIHMLPITPVSSLIRGSGFVKEEWNQQISKFISKVNSGWTGILRLNQALYDAKSSYNFFSSSSWSDNYLDNGQSRTWGLTFSAGIMNALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHN3</accession>
    <name>G3AHN3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BIR-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_65359</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373781.1">
        <property type="nucleotide sequence ID" value="XM_007373719.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AHN3">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHN3"/>
    <dbReference type="GeneID" id="18875037"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65359"/>
    <dbReference type="eggNOG" id="KOG1101">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_263316_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHN3"/>
    <dbReference type="OMA" id="MANINHE"/>
    <dbReference type="OrthoDB" id="2196114at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
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        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050784">
        <property type="entry name" value="IAP"/>
    </dbReference>
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        <property type="entry name" value="DEATH-ASSOCIATED INHIBITOR OF APOPTOSIS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="INHIBITOR OF APOPTOSIS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="214"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="599"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="664"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="955"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="459"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="546"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="603"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="678"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="739"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="818"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="832"/>
            <end position="850"/>
        </location>
    </feature>
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        <location>
            <begin position="853"/>
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        </location>
    </feature>
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        <location>
            <begin position="864"/>
            <end position="881"/>
        </location>
    </feature>
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        <location>
            <begin position="913"/>
            <end position="926"/>
        </location>
    </feature>
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        <location>
            <begin position="960"/>
            <end position="981"/>
        </location>
    </feature>
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        <location>
            <begin position="985"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1039"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1112"/>
            <end position="1134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1298" mass="147719" checksum="041011C1729E31E9" modified="2011-11-16" version="1">MAQDDNMIYRENRQQTFEEPIEQSWGAVDWKQEQNEAWTDELVNSGFYYAPTLMDKSRVVCAYCNGEEILDEIADPLDVCQVHASKGGCLWSLARVAMVDSLRYKTKRSNKSYWKRQKHTILNDPLSEEAIDFRKGFFTQTYPLDIGDQRPNSRSLSEAGFIYSPNHVGDDRVVCIYCGCALDYWESGDDPVEEHKKNETSYCYFLDTHTEQNKKRRRRKKAPVVEDNSQIESKEESVEPSIKQISPGYIEKEELVESIKQSSPIVIEDEEEPQKTPPIEVDNYTFGKETKSPYTDLNNSSTILQSQATYKSKAVKRRFKERDLNVDDLSSDDIANDFIMAAILSQDKELTPKKKKAREVTPQSIEDISEEDPISSYNTNKDFQEDPTSTLNTDNDSQEEVPISTNITKKESQENGSVSIKSTQESGISVDSENSVNYSPVELEFDSEESQEAQPVDSASEEEEESQSEESQDESSQSASEDEDESVESSIIETQSDDDYVESDDSEVIEIEESTPEKKLHKKKIMGISPRKEDDLSGSRTRRKRLPEPVEPEPKIPKKQQKLNLKKLQSVTRNDIDTSFDNIDYGEGNVEQLEKIIITSQIEPERSPSDKVPREKDIPIPKISPIKFAGQKRTTKRTSIVKSQPSIFDVSFEAPPKRLTLGRIETPEEKDEMILDNLEDKDQKALQETIEHKPEPVIEKRRTRQQSKAQKIVNDDMSAGKSKSNEKEKPKRSQRNNKKKEEPRNESAEIVDEKISNSGKSILNGNEGGKKSRQNKKQEEASNHSVILMEGKDENSYDNTQQVKKKVRQSKVEKDIESEVEEEQEPDEQPEEEHPKEEEIENNSKDKEIESEQLVQVRNSLPSPKKEVLELHKPSNGKLDHSINLQSPKSSDHVSDSEQLDEVSIKEKYIDAFEPDLDDSESDNEQDYANYLRDIQEISEDIDKLYDADGKTINSISFVQREENDKMDKDLSSDESEKSEEPEQENTSPSSPKPSNAFGNRKSSSIIPVDKEMQVSRETSIVVKEMSSPQAHSTLAKIEPNKETIADKEDIPEVDHEKNSELEDSKPEVDQPTIEIDRPNNSVSQDPKSEEDIQERQSNVEVDDNENSVLENPKELHTQERQPNEGKTSRKELILPKLPGSNEPITSPLAMCSSPMLGSISATKDNDPLSEASETQVQKPLNDGNALNLQANGHKMKWEAKPITEMLAKMSYLQSSAEEVKKLANSNYGLHDDVNGDLTRFISEMPEEEEGMTIKEWIEHCAKNCYDIVVQNGDETREFLKEEYLRAIATIEELPTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHN6</accession>
    <name>G3AHN6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Outer spore wall assembly protein SHE10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_65362</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007373722.1"/>
    </dbReference>
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        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="GeneID" id="18875038"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65362"/>
    <dbReference type="eggNOG" id="ENOG502QT2T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012877_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHN6"/>
    <dbReference type="OMA" id="WTFVCPL"/>
    <dbReference type="OrthoDB" id="3260408at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="608"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="708"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="536"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="622"/>
            <end position="639"/>
        </location>
    </feature>
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        <location>
            <begin position="643"/>
            <end position="654"/>
        </location>
    </feature>
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        <location>
            <begin position="663"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="708"/>
            <end position="736"/>
        </location>
    </feature>
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            <end position="751"/>
        </location>
    </feature>
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            <begin position="752"/>
            <end position="784"/>
        </location>
    </feature>
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        <location>
            <begin position="788"/>
            <end position="816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EGW34200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="831" mass="95203" checksum="01E6A0A38D5A0853" modified="2011-11-16" version="1">MNYKFTWVATVLSIYVFYAWTFVCPLNAPSSLSVNGDLPPVSYHFCTVSNGYLKPIYQEQVIPLYQSKVVPVFSSVNEQFGVVSKVTTVVKFVETVDAKFNISGSIGPVAEKAAVLIKQFLEKYIIPYVEKSSEISKQKFDLYYNIVKNFVVFTWDQLLETLQTSVLPYFTPLVRSFTSLEVVQWAIDWYYRLFNQVVNSKHAAKLQEKSSFLKNEFNNLFKFEEFYPRIPKTRNALQVVKDILSNIGGTNANEESDESDVETVYLTSTVRVTQSNSVATEDPVIAQLQSEILYWENKVNKTVELAMNNLEVEMKPLIDALIEKIKPNISQQLQDLQRGHHLQYQTLNKKISEINRDYEKIKETNDTTIETITRQEIRDDIAASYKLAEDTSESVQKILIDSHQYILEEYFRITQQTIDVLETFSETTMAQFSKRLGKMIHDLELDDDQISWAIWKRFHVVKESLFEFRDFILDSANEYKNTNSKDDKVIGMKVWNEYLRNVEFHLNFLLRDNADYLQLMRAKANIAFQMREGLVYDLKKEEAREQEEQEKKEQARKETLKKIEERMEKEKQEKEIARKAKAEKERIKVQQLEDELWEEIVGEKKRAEARISSELTEETDNSETKQLEYSQEKSSREQSSEQVTVQSTPQQTEPLHTAIEESLFVEESESPDSPEFSSSEIESREITTIETSLESIEDIVESSISLLESSTASEVLESTIGSETSSAEESSEVIIENTPSDDSSLIESSYTGSISEVSESPISSEIESSELSETTTSGLLVSSEIKVSESTLESGPETSELSVSSSADDNETNTLSEPKLNETFIESLSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHT8</accession>
    <name>G3AHT8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Actin-related protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_54412</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373836.1">
        <property type="nucleotide sequence ID" value="XM_007373774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHT8"/>
    <dbReference type="FunCoup" id="G3AHT8">
        <property type="interactions" value="729"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHT8"/>
    <dbReference type="GeneID" id="18871859"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54412"/>
    <dbReference type="eggNOG" id="KOG0681">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008246_1_0_1"/>
    <dbReference type="InParanoid" id="G3AHT8"/>
    <dbReference type="OMA" id="YPFTEHV"/>
    <dbReference type="OrthoDB" id="7340501at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10211">
        <property type="entry name" value="ASKHA_NBD_Arp5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000122">
        <property type="entry name" value="ARP5 actin-related protein 5 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="288"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="377"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="412"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="496"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="290"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="419"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="764" mass="87922" checksum="235384002C1BA3E8" modified="2011-11-16" version="1">MSPSKAKPEEPEFPPQPVNILTDYHVSTTTPEPFYTSYTPNTPIAMDIGSSQFRIGLTNSSSPNNVFPSVVSRYRDRRAQATLTLIGNDVYGDPALKSTIKTPFDGPLITNWDYIEYMLDYSFEHLGVDSDNGRVNNPIIMTEPLGCPLSQRRGMYELLFEAYRVPKVVFGIDALFSYYANKGKDGVVIGTGHESTYVIPVVSGKGVMSQSKRIDWGGDQCQNFLGKLLSLKYPYFPTKLNSHQTTNLFKDYCYVSENYQEELKHILDMDVLEQKDIVVQVPVEIASNPEPKKSEEEIAKQQQKRKEQGKRLQEQAQQKRLEKQAQKKEEWEYYSKFKTEMVNLTKEQIEARLSEDGFDDLDDFNKYMVSLEKSVKRADHEEQEDEFDAGSAWPLVDTPDDQLTDEQIKEKRKQKLHKANYEARERTKELKRQEEEAKAAYEKEQQEWRERDLDDWCTTKRVQLASAISKYKERQKLLESFKDRKSAAAQQRMKNIADLANDETGSTSATQRKRRRNANATIDNDPNDTFGANDDDWNLYRDISNTSIEEEMTNIMTEITNLEDDLLKYDPNFHHEDTFAAATTFDWSSSVLHKFIHGPRPNMTIAMQAEGLSPEEIASHPEVIRKNHQMHLNVERIRVPEILFQPNIAGLDQAGISEICSGLINRRLEREAAERVIKDVFITGGLAKLPNFQTRVERDFRSFLPVGTPLKVRTARDPILDAWHGMQMWADSEECKSGYITKKEYEEYGAEYIKEHGLGNMCLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIC7</accession>
    <name>G3AIC7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein SPT3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_59065</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SPT3 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373280.1">
        <property type="nucleotide sequence ID" value="XM_007373218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIC7"/>
    <dbReference type="FunCoup" id="G3AIC7">
        <property type="interactions" value="1131"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIC7"/>
    <dbReference type="GeneID" id="18872750"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59065"/>
    <dbReference type="eggNOG" id="KOG3902">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038706_1_1_1"/>
    <dbReference type="InParanoid" id="G3AIC7"/>
    <dbReference type="OMA" id="QFMFNEQ"/>
    <dbReference type="OrthoDB" id="66982at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22926">
        <property type="entry name" value="HFD_SPT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000023">
        <property type="entry name" value="transcription initiation protein SPT3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003195">
        <property type="entry name" value="TFIID_TAF13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID/SUPT3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380:SF16">
        <property type="entry name" value="TRANSCRIPTION INITIATION PROTEIN SPT3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02269">
        <property type="entry name" value="TFIID-18kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="91"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="37303" checksum="6BB4AD2D78FE6E0E" modified="2011-11-16" version="1">MSESKYKYRIEIQQMMFVSGESNDPPIETTSIIEDIVRGQVIEILMQATKTASSRGSKSIAPEDVIFMIRHDKAKVNRLRTYLSWKDVRKNAKDQEGGGTESLIEGDAAAGTGGTGAAAAGANQSDSSKMLTRYRKSKIRLPWELQFMFSEQHLETGEDDQIDDEEKAATIASLKRLKTADDRTKNMTREEYVHWSECRQASFTFRKAKRFREWAQIGQLCDSRPNDDVIDILGFLTFEIVCSITEEAMLIKNSEEKLIQIRKQKNVDDNVPKKRKYLFDKPDELANPLLPYHIEEAWRRLQANDFKHKAARSFGGGLVRTRTRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIN9</accession>
    <name>G3AIN9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Intermediate filament protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_149358</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374039.1">
        <property type="nucleotide sequence ID" value="XM_007373977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIN9"/>
    <dbReference type="FunCoup" id="G3AIN9">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIN9"/>
    <dbReference type="GeneID" id="18870822"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_149358"/>
    <dbReference type="eggNOG" id="KOG2101">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002131_1_0_1"/>
    <dbReference type="InParanoid" id="G3AIN9"/>
    <dbReference type="OMA" id="AMYVVEV"/>
    <dbReference type="OrthoDB" id="120967at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06876">
        <property type="entry name" value="PX_MDM1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003114">
        <property type="entry name" value="Phox_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013937">
        <property type="entry name" value="Sorting_nexin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF3">
        <property type="entry name" value="SORTING NEXIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08628">
        <property type="entry name" value="Nexin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02194">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00313">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51207">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="PXA" evidence="7">
        <location>
            <begin position="90"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="5">
        <location>
            <begin position="342"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="6">
        <location>
            <begin position="646"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="518"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="571"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="986" mass="114105" checksum="CDCD4705BC418391" modified="2011-11-16" version="1">MSFLSVKIGTIIILWYIMSYKVSFGVFILGSLTAITSIGYLILTFKPRRRLTQAPSKHNRFKFTLPEQWQIELKSFETDKQELHKPTTDSLIISTVLDELIQLIIKEFIQSWYKEITQSSLVTDSIAIEIKQVIRNIIDRLEKIDFTKLLVSRILPIIQDHFQASSQAENYVKAKGNFSKFDSRDFQLAVAQQYKRGKLHRAVTVASAQPLDINQKKYLRGKIGACLPFILSDNERDNEVGVSLVTEIIACTVLSNIVNLVTEADFYNVIIVKLIGDNLRRRDQVKQLRAALEEHTQLKRPLKEVPNFDVDIDSLSTSIETTTDITSKKQQLTLSNLKGSITLRDILSNPSKLIIFREFLTKKVKESFVDFWSSVEAIKSPLEEETTLLLEYTDINDIHDIYSRYIEPGIIRANNKEIFVDLFNSKEEDKLNLYHEARTYLLQLQQEVYSIIDNEYLPLFKESEFFENLITGNNESSQPVPVNPEIVHAVESAFSQIMSKQEIQDEQMMSKKELFESSSSLFDEEDDDDNQSNRKLFDSDEESDSDSLNFDSDTPAPSLTDSELQLAAPGNLNLAEEIRKLNDESDNLHRQMAVLEPLIIKAELTNNLSELKVLQRSKQALIREINFKELQKQQYIVQENDNSLYGKSRVCIQSYISSNENNKEFTLYIIEVQKYSNDDPNITTAGWVVARRFSQFYRLHEYLKLKYPSVAHIKFPKRSMLKFQHQQLIEIRQKALERYLQELIKIPEICSNKAFRSFLSSENFSLHKNQPFGDAEFLANKWYRGIYDKFMPISGSHDIPNNPIEAESQIVENIRDMEKELKQFDEGTNTKTTVFVKPICDLLITVFRLSNSKSWLRGRALVVILQQLFGTTIEKKVYQTEAQFKTEQNLLELVLLLKNTIFPNGKFKDPPVLRTLYQQSNTRQEAKGLWSVFMYETCSTIFGTSNTTFASNALFRMIQNDYLNKHLVFEIFDELFNEIFPEVANS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIY4</accession>
    <name>G3AIY4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thiaminase-2/PQQC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_54081</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373380.1">
        <property type="nucleotide sequence ID" value="XM_007373318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIY4"/>
    <dbReference type="FunCoup" id="G3AIY4">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIY4"/>
    <dbReference type="GeneID" id="18871835"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54081"/>
    <dbReference type="eggNOG" id="ENOG502QT8P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077537_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIY4"/>
    <dbReference type="OMA" id="AEWITLH"/>
    <dbReference type="OrthoDB" id="270009at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19358">
        <property type="entry name" value="TenA_E_Spr0628-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026285">
        <property type="entry name" value="TenA_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004305">
        <property type="entry name" value="Thiaminase-2/PQQC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050967">
        <property type="entry name" value="Thiamine_Salvage_TenA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43198">
        <property type="entry name" value="BIFUNCTIONAL TH2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43198:SF2">
        <property type="entry name" value="SI:CH1073-67J19.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03070">
        <property type="entry name" value="TENA_THI-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003170">
        <property type="entry name" value="Pet18p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiaminase-2/PQQC" evidence="3">
        <location>
            <begin position="12"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR003170-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR003170-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="230" mass="26844" checksum="E82009B40B31B1F1" modified="2011-11-16" version="1">MSIVEKLLRKYADLYTESTTHPLTNELCNGTLSDHRLFTYLTQDLKFFQIGLNLFGNTLAYCDDPQAAIVLGKQIGFLSNDENDYFFKSLSQIKEENLQLLQEKVGDMLGDNPVTLPEVEKYLDLLKYMTYQSKSYVELITFTYVMEKVYLGWADYNIDNGLVKQGLEFKHQEWIDLHSGEAFTKWVEFLKQEVDRVVKTEQDEKVCEEMFVKALKQEISFFDGCYNYGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ27</accession>
    <name>G3AJ27_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_54658</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BMT family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374124.1">
        <property type="nucleotide sequence ID" value="XM_007374062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ27"/>
    <dbReference type="STRING" id="619300.G3AJ27"/>
    <dbReference type="GeneID" id="18871878"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54658"/>
    <dbReference type="eggNOG" id="ENOG502QTZG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013841_3_0_1"/>
    <dbReference type="InParanoid" id="G3AJ27"/>
    <dbReference type="OMA" id="MVNRIVY"/>
    <dbReference type="OrthoDB" id="6335297at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021988">
        <property type="entry name" value="BMT1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12141">
        <property type="entry name" value="BMT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW34540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="531" mass="60988" checksum="857539A2C22A0D36" modified="2011-11-16" version="1">MTVEVPNIELSTQTAYLAALTIFTPIAIFFVHKSFSPKTRDGKTKHNSPISANRSKLKFEKFKVPGYSFIETGEESTDSRFKCTQVEFHDDENLSATVATNLNNNDDLKFLRNQLKELSATKQEYRDFFVDKEDETEESILANKWWKFCGSAVWLEKYNVHFMVNRIVYSKDAVRSWPTISVLSGQVFDNDWNELTDFKFPNSDLKFPGILPHIIDQGPYHKLEILGAEDPRAVLHEFTNDQGELDQEPLIIFNALRTEIGWKRAMHVYRPLQDPYNITRLSINGLKPRLIEKNWAPFMDAEEGEINFIYNFHPLRVLQCSVYTGECNKVSGPDFNKDPTKNAGKLRGGTNLVPIPASLLPKKLSHRKYWFGIGRSHTKDCGCVNELYRPHAFIISRPEDSLSFTLDYVSSLIDFNMRPETWNGGESICDDGKSVLIPNSISYWDIANTDSTEKPEDYMGITFSTADMHNKLVHVKGWLPHIYLVLDKLESQLIQHYADVDHTFIENKLLGECSTFLSEDYCEATSKLQSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ46</accession>
    <name>G3AJ46_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RanBD1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_59985</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374142.1">
        <property type="nucleotide sequence ID" value="XM_007374080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ46"/>
    <dbReference type="FunCoup" id="G3AJ46">
        <property type="interactions" value="1049"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ46"/>
    <dbReference type="GeneID" id="18873165"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59985"/>
    <dbReference type="eggNOG" id="KOG0864">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067861_0_1_1"/>
    <dbReference type="InParanoid" id="G3AJ46"/>
    <dbReference type="OMA" id="NFKDSFM"/>
    <dbReference type="OrthoDB" id="2357150at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13179">
        <property type="entry name" value="RanBD_RanBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000312">
        <property type="entry name" value="Ran binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045256">
        <property type="entry name" value="RanBP1_RanBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF87">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE RANBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RanBD1" evidence="2">
        <location>
            <begin position="53"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="190" mass="21728" checksum="6EFABF62B95C43FE" modified="2011-11-16" version="1">MSAEESSIPKPPTANVFSMFGAKKEKKEEPKEEVEDKKADDDEAAAEEEADVHFEPLVQLEKVDVKTNEEDEEVLYKVRAKLFRFHGDTNEWKERGTGDVKFLKHKQSGKVRIVMRRDKTLKICANHLISGEYELKPNIGSDRSWVYTVTADISEGEPEAQTLAIRFGNKENADLFKKHFDEAKNANKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ65</accession>
    <name>G3AJ65_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Putative acyl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_134579</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373406.1">
        <property type="nucleotide sequence ID" value="XM_007373344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ65"/>
    <dbReference type="STRING" id="619300.G3AJ65"/>
    <dbReference type="GeneID" id="18869833"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134579"/>
    <dbReference type="eggNOG" id="KOG1469">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_1_2_1"/>
    <dbReference type="InParanoid" id="G3AJ65"/>
    <dbReference type="OMA" id="WNMFLSK"/>
    <dbReference type="OrthoDB" id="434771at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF13">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0274">FAD</keyword>
    <keyword evidence="4 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="10">
        <location>
            <begin position="52"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="9">
        <location>
            <begin position="146"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="8">
        <location>
            <begin position="269"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="443" mass="49172" checksum="692A964DDF4D5022" modified="2011-11-16" version="1">MSITQDIPAIFLEKISPKGLEAIQKTKDFVHDYCLPADAIYLSQISNDPATRWTSTPPVMEKLKAKARELGLWNMFLSKHYPEGPGFTNLEYGLMARYLGRSYTAPEATNTAAPDTGNMELFAKYGTGYQKQKWLQPLLNGEIRSAFVMTEKGTSSSNALNICTSAKLNSKGNYVVNGHKWFASGAGDPRCSVWLVMCKTSDDSANPYRNHTVLVIDAKKAIASGKAKVVRPLHVIGYDDAPHGHCEIEFSDFEIPAEDMPHAVLAGVGRGFELIQSRLGPGRIHHCMRAIGAGEFALLRIAHRSNHRLIFGKPMNQRESFLTRYAQSRIDIERCLLLVLNAAHKIDISNAKAAQREIAMAKIETPRTIGAILDWGIQVFGAEGVSQDTELARMWANNRTLRIADGPDEAHLAQLARNEASRFGEVDKFFANFQAKRDRVGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJS8</accession>
    <name>G3AJS8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein arginine methyltransferase NDUFAF7</fullName>
            <ecNumber evidence="7">2.1.1.320</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_134439</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Arginine methyltransferase involved in the assembly or stability of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)'-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48108"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:88221"/>
            <dbReference type="EC" id="2.1.1.320"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NDUFAF7 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.320" evidence="7"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373563.1">
        <property type="nucleotide sequence ID" value="XM_007373501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJS8"/>
    <dbReference type="STRING" id="619300.G3AJS8"/>
    <dbReference type="GeneID" id="18869817"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134439"/>
    <dbReference type="eggNOG" id="KOG2901">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024840_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJS8"/>
    <dbReference type="OMA" id="YYHPQRN"/>
    <dbReference type="OrthoDB" id="5595109at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035243">
        <property type="term" value="F:protein-arginine omega-N symmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12710:FF:000008">
        <property type="entry name" value="Protein arginine methyltransferase NDUFAF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003788">
        <property type="entry name" value="NDUFAF7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038375">
        <property type="entry name" value="NDUFAF7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049:SF7">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02636">
        <property type="entry name" value="Methyltransf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="504" mass="57354" checksum="80F89DE16420C353" modified="2011-11-16" version="1">MLQLYKGTTRLYSRVLSRQFSNTCLLLNEKKEDINKSNYGQFTKQEFENARTIIKEQIARLESEIKGESNIRSKLGSMPQFPSPSKSTARKVDTLTDLFAQTIQLTGPIPLSAYMRQCLTHSEMGYYTTRDPLDLRGGDFITSPEISSVFGEMLGVWYFTVWQSQGSPGKIRFVEFGPGRGTLMYDTLRTFNRFIAKASTPVDIEITMIEASDVLRREQWKLLCDEKHEFETTTEGFNKSTTQWGNAIKWVYTEKDIVDDEATANYIIAHEFFDALPIKSFTKTEAGWRELMVEHTPTVTNTQPKLATTEPVEKNDLFETEFHLTVSQKETPSSMIPALSPRYRDLAQGSRIEICPDAELFLMKMIQLLKPKLGAVMIMDYGVVDSIPENSLRGIYKHKFVSPFIKPGEVDLSADVDFDNLVNISKQASQGKIINYGPVDQGDWLHNIGVGYRIDQLIKQNESNPDVQDKIYGAYRRLTDKDQMGGVYKFLTLMPEGSEKPIGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK75</accession>
    <name>G3AK75_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60228</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374401.1">
        <property type="nucleotide sequence ID" value="XM_007374339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK75"/>
    <dbReference type="GeneID" id="18873286"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60228"/>
    <dbReference type="eggNOG" id="ENOG502SS03">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1266754_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK75"/>
    <dbReference type="OMA" id="VQYIPIV"/>
    <dbReference type="OrthoDB" id="3980365at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="233" mass="26524" checksum="5022BF2B0BBF1F36" modified="2011-11-16" version="1">MPAVPVLTIPESSYDPSLYSLQTRRGKSQILIYLIKLTHGSAFTLALAYIIGLLVIKPLLETKASRRLELLESARVGLRDFYLKLIGKVSHIPIVAIRKPGDSKFYSDAVVQTHDSHVDKTRVRTDREKWEELIENDKLAQGRLHEKLKKLSTTLNDECRTYSSLDMDHYKSANYAIKDFQNKADLVYFNSTELFMQESAPTDTNKSLRKKNSAVEARNEIRSIKGLYMSGQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKI0</accession>
    <name>G3AKI0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_49866</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PAF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374453.1">
        <property type="nucleotide sequence ID" value="XM_007374391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKI0"/>
    <dbReference type="FunCoup" id="G3AKI0">
        <property type="interactions" value="308"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKI0"/>
    <dbReference type="GeneID" id="18871378"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49866"/>
    <dbReference type="eggNOG" id="KOG2478">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021991_3_1_1"/>
    <dbReference type="InParanoid" id="G3AKI0"/>
    <dbReference type="OMA" id="LVCRIKY"/>
    <dbReference type="OrthoDB" id="10260285at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007133">
        <property type="entry name" value="RNA_pol_II-assoc_Paf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23188">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23188:SF12">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03985">
        <property type="entry name" value="Paf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="396"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="159"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="396"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="406"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW32938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="443" mass="51133" checksum="DFDCAE52ED3C678B" modified="2011-11-16" version="1">MSQPSSSKPTRQDYIAKVRYTNNLPPPPLNPKFIAYNTTETTSSKVEGEQLMSSLFRKENFQSLLAQVDDFYGMGLNLIDNSGFLDLGNDSVISGKTGNIQLHPKDRALLRDAGIGKISKSEPGVSFLRRTEYMSEARPSSKGGAVDELQKKETTRVKKIAEEEEMMDADSQLKAVEDTFDKAQESLVNFDKIRHPKKKHLRAVDVWPLLPDTSMMDNKFLMVKFTGSASIHREIEMLKRTQGNKFNEKFVRDSLESSILKPITSDDGEWISLFQVKDEKKASELNERLKSTERERPVNLLDEEEDVLEEYKFKYVKNYDMKFQRNKRETEELGIKFIPEEGTKKRKVAYFYPIAGKIDLKKHRASTNSEINRFLRDSTLDGINFKLREPTTNELKRMDNIRSEFDPMEYEGEEEEEEEDEDAKSDEDSDKDVSDEFNEAEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKI6</accession>
    <name>G3AKI6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cystathionine beta-lyase</fullName>
            <ecNumber evidence="3">4.4.1.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cysteine-S-conjugate beta-lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_60930</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L,L-cystathionine + H2O = L-homocysteine + pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13965"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an S-substituted L-cysteine + H2O = a thiol + pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:18121"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29256"/>
            <dbReference type="ChEBI" id="CHEBI:58717"/>
            <dbReference type="EC" id="4.4.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homocysteine from L-cystathionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.13" evidence="3"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375106.1">
        <property type="nucleotide sequence ID" value="XM_007375044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKI6"/>
    <dbReference type="FunCoup" id="G3AKI6">
        <property type="interactions" value="152"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKI6"/>
    <dbReference type="GeneID" id="18873639"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60930"/>
    <dbReference type="eggNOG" id="KOG0053">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_2_1_1"/>
    <dbReference type="InParanoid" id="G3AKI6"/>
    <dbReference type="OMA" id="NCIGATG"/>
    <dbReference type="OrthoDB" id="2545919at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047804">
        <property type="term" value="F:cysteine-S-conjugate beta-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071266">
        <property type="term" value="P:'de novo' L-methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000013">
        <property type="entry name" value="Cystathionine beta-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000009">
        <property type="entry name" value="Cystathionine gamma-synthase homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006238">
        <property type="entry name" value="Cys_b_lyase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01329">
        <property type="entry name" value="cysta_beta_ly_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF50">
        <property type="entry name" value="CYSTATHIONINE BETA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="5 13 14" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="13">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW33591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="404" mass="45060" checksum="B56E8D8B2A6A0E85" modified="2011-11-16" version="1">MSPKKSYSLETELVFTKSGDQYNASVAPLYQSATFKQDSLSQMGEYDYTRSGNPTRTHLQNHLAKIMKAQSAFAVSSGMGCLDVITRLLKPGDEVIAGDDLYGGTHRLLTYLNKKGDLIAHHYDTTNTELIKSKITDKIKMIFLESPTNPLIKVVDVKSITEHAHKVNPDIVVVFDNTMMSPILMTPLELGVDIQYESATKYLNGHHDIMAGVIATRSTKLAEQLYYIINSTGCGLSPFDSWLLSRGLRTLAIRVERQQENCVRIAKFLHQIGFKVRYPGLKSHPQYELHSSMCRGYGAVLSFETGNIKLSEKIVESTDIFGIAVSFGCVNSLISMPCKMSHASIDKKTREEREFPEDLIRLCIGIENVDDLIDDLTKALLKSGAVKINDRDELYNAVGLQPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKX4</accession>
    <name>G3AKX4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Meiosis-specific serine/threonine-protein kinase MEK1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_150549</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHEK2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374532.1">
        <property type="nucleotide sequence ID" value="XM_007374470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKX4"/>
    <dbReference type="FunCoup" id="G3AKX4">
        <property type="interactions" value="221"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKX4"/>
    <dbReference type="GeneID" id="18870869"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_150549"/>
    <dbReference type="eggNOG" id="KOG0032">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="InParanoid" id="G3AKX4"/>
    <dbReference type="OMA" id="EDQYTIT"/>
    <dbReference type="OrthoDB" id="74764at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22670">
        <property type="entry name" value="FHA_MEK1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FHA" evidence="6">
        <location>
            <begin position="40"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="138"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="435" mass="50059" checksum="50020411B38A34FF" modified="2011-11-16" version="1">MSLAGVLEITELNKTKVNTSIDITHNEDFVEIPIYVQQVLTLGRDQSCDVQLNNYVISSIHATIWSIQFDNDSISLVYMHDNSRNGIIHNGVEISKGMTVILQDGDTIEFKLAAKFQYKSFQLESCYSLELGCFDDWKVTNNLIGSGSFGSVYVANRQHDRRLYAVKIIPTQPSYIPDKLIKQESTLLLNINHVLHDAITYDSNIYIFEDLICGGDLFSYLRTGKYLRALPEQETISIVYQLLKAISYLHKELQIVHRDLKLDNILLEIPVPRTRIYLCDFGIAKCLSNCKQQKTDTCVGTIEYSAPEVFQEEREYSFKCDLWSLGVICHILLSGISPFYGNGEKEFMITAARTGRVNLNRHQFRNSSSMCKSFIQRLLKVDPDERMSIDDCFNHEWIRINQTNLEKFYQKRIAKNLDTKRSDQKKNRASELNIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKY4</accession>
    <name>G3AKY4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Serine/threonine-protein kinase TOR</fullName>
            <ecNumber evidence="14">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_136422</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Vacuole membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the PI3/PI4-kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="14"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374542.1">
        <property type="nucleotide sequence ID" value="XM_007374480.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AKY4">
        <property type="interactions" value="1153"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKY4"/>
    <dbReference type="GeneID" id="18869970"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136422"/>
    <dbReference type="eggNOG" id="KOG0891">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000178_7_1_1"/>
    <dbReference type="InParanoid" id="G3AKY4"/>
    <dbReference type="OMA" id="MRQHSAK"/>
    <dbReference type="OrthoDB" id="381190at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016242">
        <property type="term" value="P:negative regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905356">
        <property type="term" value="P:regulation of snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05169">
        <property type="entry name" value="PIKKc_TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000020">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.150:FF:000001">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000371">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000006">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.150">
        <property type="entry name" value="FKBP12-rapamycin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009076">
        <property type="entry name" value="FRB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036738">
        <property type="entry name" value="FRB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057564">
        <property type="entry name" value="HEAT_ATR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024585">
        <property type="entry name" value="mTOR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026683">
        <property type="entry name" value="TOR_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF9">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08771">
        <property type="entry name" value="FRB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23593">
        <property type="entry name" value="HEAT_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11865">
        <property type="entry name" value="mTOR_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01346">
        <property type="entry name" value="DUF3385"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01345">
        <property type="entry name" value="Rapamycin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47212">
        <property type="entry name" value="FKBP12-rapamycin-binding domain of FKBP-rapamycin-associated protein (FRAP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8 14 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="FAT" evidence="16">
        <location>
            <begin position="1350"/>
            <end position="1930"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="15">
        <location>
            <begin position="2104"/>
            <end position="2418"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="17">
        <location>
            <begin position="2449"/>
            <end position="2481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU364109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW33027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="2481" mass="281188" checksum="C8E4A1883C8578DF" modified="2011-11-16" version="1">MSSSATAVATTAPAVQVVDPVLSQIFNGLKSPREEERSRYALELRNYLTSMARDLSSEQFNRLDADINRKIFDLLHLEETSQILGGIAALNALIDFDSGVGKENASKTARFSNYLGSLILSNELIIMKQATKTLGKLSTLGGNLTGDFVDYEAKRAIEWLSDNTKQHENRRHAAILIVTALADNAPTLLYPLINDILKHLWVPIRDQKLIIREDAALALEKCMIIIYDRDVNLRNHWIKRMIDQASKLLNENDISDASDANGGAPGITAATYLIPSASSSQSTENIHGSLLIYRELLKFYRDPYIFSKFDQMYENTILYKHHKLAIIRQELTNIFPLLCKVNTELFVEKYLHRTLYYYLSQLKKYKGQHSETANGDKSAIFKSIGLLALEVGNQMATYLDAVLDNIREGLSYAFNAGVQSILANAVSSNETPNINLTPSSSLTSGSKYTVSRRETEPAIFDCIGKLSISVGPALTKHLQRDILDMMFANCSLSQHMQDVLQTLITNIPTLTNLINEKLLNLLSLVLSGKGFQPPGSPYGSVKVNASLARDCRLIMISRDTGLSINNVLLNQEQYERLDSQILIQALQMLAFFQFENYQLNEFVRYCTITYLEHSNPQVRLTATVTSCEIFVKDPICQQISANALNAVNEVLGKLLSISITDPTPEIRLQGLNCLANAGNFDPQLSQADNVRLLFIALNDEEFAIRQVAVRILGRLSSINPAYIVPSLRKTLIQLLQRLEYSTTSRKKEESAILLSLLIANSKELARPYVKPIVEALITKAKDPSSSVASSAINCLGELAVVGGEDLKPFIPSLLPLILDTFQDQSSSYKRDAALKTLGQLASSSGYVIQPLLDYPQLLGMLVGILKSETSPKIRRETVRLLGILGALDPYKHREVEQNSQNIPAEQNAPSIDVALLMQGMSPSNEEYYPTVAITNLMKILKDPSLNIHHTKVIQAIIYIFQTLGLRCVSFLPQIIPGVLNVMHTCQHPMLKFYFQQLGAMILIVKQHIRPFLNDIFEVIKEFFHTQPNTLQVVIINVIESISRALEGEFKMYLPGILTLLLEVFEEDKSPKRTPSSHVLRSFVVFGSNIEEFIDIIVPNVVKLFEVGPIELRKLAIETIGRLSKNVMLNEMASRVIHPLLRVLRQGNDELKESCINTLSYLLIQLGSEFTVFIPVIKKEILQQKIHAPKFESLVSKLISGDPLPLHLDIYKDYDQSSYDVADSDMPSKKLPVNQNALKNSWDVSHRRTKEDWQEWIGKLSKELLLQSPSHAIRACAGLASDYYPLAKDLFNASFASCWSELYSQHKEELVQAFCIALSSPSNPPEIHQILLNLAEFMEHDDKSLPIAITTLGQYAQRCHAFAKALHYKELEFYEEPTTPTIESLISINNQLQQSDAAIGILKHAQLHHDLQLKETWYEKLQRWDDALNAYNEREKTEPNNMEITMGKMRCLHALGEWEQLSELARTKWDSSSSDIKRNVAPLAAASAWGLGQWDRMDSYIKVMKPESPDKAFFNAILSLHRNNFEDASNHILKARDLLVTEITALVSESYNRAYGVVVRVQMLAELEEIIKYKRLPQGSEKRAIMRKTWNTRLLGCQRNVDIWQRMLKVRALVIKPKQDMDMWIKFANLCRKSGRLNLAEKSLNSLLEEGSPENPSRAPPQVVYAQLKYMWAKGQRPEALRHLVDFTTRMSQDLGLNPNDLITQPLPSEGPGIPKHVEEYTKLLARCFLKQGEWQIALNNNWRTETSEIILGAYLLATHFDNKWYKAWHNWALANFEVISLYTSQNAGNSNKIEIVDQNETSANGDDESQAKQPNQPNIIPIEAVQRHVIPSIKGFFHSIALSHSNSLQDMLRLLTLWFKFGGIPEAAQAMTEGFNMVKIDNWLEVIPQLISRIHQPNQIVSRSLFGLLTDLGKAHPQALVYPLAVAITSESVSRKIAAQSIIEKIRLHSSTLVDQAELVSRELIRVAVLWHEQWYEGLEDASRLFFGEHNTEKMFEVLEPLHRMLQKGPETMREQAFANAFGRELADAYEWVLNFRRTKDITNLNQAWDIYYNVFRRITRQLPQLVSLELQFVSPNLERAYDLELALPGTYEAGKPVIRIIRFDPTFSVISSKQRPRKLSCKGSDGKDYQYVLKGHEDIRQDNLVMQLFGLVNTLLVNDPECFKRHLDIQQYPAIPLSPKVGLLGWVPNSDTFHVLIKGYRESRSIMLNIEHRLLLQMAPDYDILTLLQKVEVFTSALDNTRGQDLYKVLWLKSKSSEAWLDRRTTYTRSLAVMSMVGYILGLGDRHPSNLMLDRITGKVIHIDFGDCFEAAILREKYPEKVPFRLTRMLNYAMEVSGIEGSFRITCEHVMRVLRDNKESLMAILEAFAYDPLINWGFDFPTKAVAEATGIRVPQVNTADLLRRGQIDDREAARLQKQNEMEIRNARAALVLKRITDKLTGNDIKRLRGLDVPAQVDKLIQQATSVENLCQHYIGWCSFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKY6</accession>
    <name>G3AKY6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">T-complex protein 1 subunit epsilon</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">CCT-epsilon</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_60350</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374544.1">
        <property type="nucleotide sequence ID" value="XM_007374482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKY6"/>
    <dbReference type="FunCoup" id="G3AKY6">
        <property type="interactions" value="1349"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKY6"/>
    <dbReference type="GeneID" id="18873358"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60350"/>
    <dbReference type="eggNOG" id="KOG0357">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_7_2_1"/>
    <dbReference type="InParanoid" id="G3AKY6"/>
    <dbReference type="OMA" id="SHPQMPH"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03339">
        <property type="entry name" value="TCP1_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000003">
        <property type="entry name" value="T-complex protein 1 subunit epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000049">
        <property type="entry name" value="T-complex protein 1 subunitTheta, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012718">
        <property type="entry name" value="Chap_CCT_epsi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02343">
        <property type="entry name" value="chap_CCT_epsi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW33029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="546" mass="59763" checksum="0FD2CD32C53953F7" modified="2011-11-16" version="1">MAAQGMPDLSNAIVAQDEMGRPFIIVRDQGKKQRKHGIEATKSHILAARSVASIVKTSLGPRGLDKILISQDGDITITNDGATILTQMDLDNQIAKLLVELSRSQDDEIGDGTTGVVVLASALLDQALELIEKGIHPIKIANGFDEACKIAIDQLDKVADVVPIEDESYLLKAAKTSLGSKIVSKSHDHFSQMAVDAVMAVADLERKDVDFELIKIEKKVGGSIEDSLLIKGVLLDKDFSHPQMPKQVKDCKIAILTCPFEPPKPKTKHKLDISTVEEFKLLQEYETKKFQEMIDLVKQSEANVVACQWGFDDEANHLLLSNGLNAIRWIGGSELELLAIATNGRIVPRFEDLSPEKLGSAGIVRELEFGTTRDRMLVIEDCSNSKAVTCFIRGSNEMITAEGVRALHDSLCVVRNLIRDNRVVYGGGAAELTCSLAVSEAADKQKGIDQYAFRAFASALDTIPMTLAENSGLDPIETLSHLKSKQVNENSSRLGVDCLGKGTNDMKELFVIDPYIGKRQQLLLATQLTRMILKINDVIISGKDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL53</accession>
    <name>G3AL53_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTH APSES-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_66101</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EFG1/PHD1/stuA family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374611.1">
        <property type="nucleotide sequence ID" value="XM_007374549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL53"/>
    <dbReference type="STRING" id="619300.G3AL53"/>
    <dbReference type="GeneID" id="18875083"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66101"/>
    <dbReference type="eggNOG" id="ENOG502QW2C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_717881_0_0_1"/>
    <dbReference type="InParanoid" id="G3AL53"/>
    <dbReference type="OrthoDB" id="5407653at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.10">
        <property type="entry name" value="Transcription regulator HTH, APSES-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029790">
        <property type="entry name" value="EFG1/Phd1/StuA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018004">
        <property type="entry name" value="KilA/APSES_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47792">
        <property type="entry name" value="PROTEIN SOK2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47792:SF1">
        <property type="entry name" value="PROTEIN SOK2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04383">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01252">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH APSES-type" evidence="6">
        <location>
            <begin position="289"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="117"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="248"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="398"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="121"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="140"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="171"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="248"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="261"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="412"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="467" mass="52227" checksum="3CBC47B084BD56C4" modified="2011-11-16" version="1">MTKTGHPNFYTSELITNPSGTEQIVGSIPQTQHLQHTTTTNEPTTEVPQNYQQYSTMLRTAKPKTLFTEQGYYSLQQGHPIPQNGEYYANPELQNGMGNNISPSFPMTSITTPESQNGFLQQQYQKQQKQQQAYQPHDPYSNSMYGGMSNGKQFLSRTRNRSTPVCSTLGNQQQNSDNNNQQLALPGGPVAPAGPTLYNSNSAPMLYTQQPQMQMSYSPAGNATNTSSGNQIYSLQGPHTLLNLPVRNSISVPGTQPNGTRASSRSSHSRSNSKDNGQPGEYPDVVRPKIATTFWESENTICYQVRSRGFLVSRREDTNYVNGTKLLNVIGMTRGKRDGILKTEKTKIVVKVGSMNLKGVWIPFDRAYEIARNEGVDGLLYPLFVKDLKSYYMKEGHKLKSEDNEDEDGHSSAGSKLMNSDNPIEHMTDSTAKPNMFRDEYYDRSVKEEELSYYGTAQNNTPMNKPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALB8</accession>
    <name>G3ALB8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60471</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374676.1">
        <property type="nucleotide sequence ID" value="XM_007374614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALB8"/>
    <dbReference type="STRING" id="619300.G3ALB8"/>
    <dbReference type="GeneID" id="18873428"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60471"/>
    <dbReference type="eggNOG" id="ENOG502S09K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071379_3_0_1"/>
    <dbReference type="InParanoid" id="G3ALB8"/>
    <dbReference type="OMA" id="PEVIPEW"/>
    <dbReference type="OrthoDB" id="5580261at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018811">
        <property type="entry name" value="MRX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28002">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28002:SF1">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10306">
        <property type="entry name" value="FLILHELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="252" mass="29062" checksum="B8B51E3275311B4A" modified="2011-11-16" version="1">MLVPTRLIAPTRILTCQFHSSPAAYKLFGKKKSETLKKPEEVKAQDPEKPKVPRAKSVFELPEFKVTPEILAKQQQQQEKPNPALYTTHPIIKRVPKFFRPYVGGFINAPYSHVISFMILHEITAIVPLFIIWWAFHQQPDYIPEVIPEWAISRATAIIENWLGNYVTINNFSDKVQFILEGAYAYTIVKVTLPLRLFASFALMPFGARLIVPIFNRFRIKKIDMYKTPQVKLVEMQVDPKAKVKNVKGKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALF5</accession>
    <name>G3ALF5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DUF1640 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_60528</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374713.1">
        <property type="nucleotide sequence ID" value="XM_007374651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALF5"/>
    <dbReference type="FunCoup" id="G3ALF5">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALF5"/>
    <dbReference type="GeneID" id="18873450"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60528"/>
    <dbReference type="eggNOG" id="ENOG502S831">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056763_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALF5"/>
    <dbReference type="OMA" id="RSEKINM"/>
    <dbReference type="OrthoDB" id="5424147at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024461">
        <property type="entry name" value="CCDC90-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360:SF12">
        <property type="entry name" value="MOZ PROTEIN REPRESENTS A CHROMATIN-ASSOCIATED ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07798">
        <property type="entry name" value="CCDC90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="36917" checksum="10A4EA46B5EBA32C" modified="2011-11-16" version="1">MIRQTPFLRLRAKLNQPISPYIAYATHTQLPIIRSKYILRCFYTFNPILQQQQSSQQTPINISQNTEPIIKADNPIYKENKPTEPTEAPATTPEDDTLCSIFEGMEPYMDTYSVYTKLTAAGFTPTQADKVISLLICQLNSKLSKLPSKYSQNYELENEQYLFESAQQELRVDITRSREQHIHELIELINVLERDFSTITDELNSDFIQMRNDAQVAINDQKSENTLNSKGMFLRIQETNHKITTELNSAMRSEIESLRWYLSRWGIITIMISIFASCTTFYISRSNSAKAEARSDFAPLMIYEPSEYEDEEDSDDYNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALH7</accession>
    <name>G3ALH7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_60558</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374735.1">
        <property type="nucleotide sequence ID" value="XM_007374673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALH7"/>
    <dbReference type="FunCoup" id="G3ALH7">
        <property type="interactions" value="99"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALH7"/>
    <dbReference type="GeneID" id="18873462"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60558"/>
    <dbReference type="eggNOG" id="KOG2317">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_694773_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALH7"/>
    <dbReference type="OrthoDB" id="686384at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017178">
        <property type="term" value="F:diphthine-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06155">
        <property type="entry name" value="eu_AANH_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06156">
        <property type="entry name" value="eu_AANH_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030662">
        <property type="entry name" value="DPH6/MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196:SF2">
        <property type="entry name" value="DIPHTHINE--AMMONIA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196">
        <property type="entry name" value="DOMAIN OF UNKNOWN FUNCTION 71 DUF71 -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="397" mass="44297" checksum="4CF76E1A77E61D37" modified="2011-11-16" version="1">MKVELVDKQEESFDLLEAPPILEDQFEEVLESLSEPTVDISALSLDTQQLSTIPQISVVNARTKLYVSNIVSTEKDLQAQASDIFFQLKTILSKHELTLNDVQSITLLLSDMSKFGEINKIYGESFIGIYLPPSRICIETTISSDVQLSCVALKKIEPKTGIHIRSRSYWGPQNIGPYSQSIVDNQEHYKLASLSGQIPLIPSSMELSSKGIKFNSALSLQHLFRVKNLVNVKNLASVICFVTDKRNVPDASSSWNEFIDSVEQAPANKKGLVIVQVSALPRGADVEWGGYSYENVVGMYDDSDDEDNEESPYDSLFDEFEHHSVCAIGKNNVLVATLFTSDINLVEKAIDINNGKNFIQVLSSKLEKPNCEFIPVKSVFNNEGQQFAYGIIWKIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMA6</accession>
    <name>G3AMA6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_60764</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374919.1">
        <property type="nucleotide sequence ID" value="XM_007374857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMA6"/>
    <dbReference type="FunCoup" id="G3AMA6">
        <property type="interactions" value="386"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMA6"/>
    <dbReference type="GeneID" id="18873554"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60764"/>
    <dbReference type="eggNOG" id="KOG4727">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1708626_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMA6"/>
    <dbReference type="OMA" id="KWNVELY"/>
    <dbReference type="OrthoDB" id="30343at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040107">
        <property type="entry name" value="Snu23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45986">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45986:SF1">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="118"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="154" mass="17526" checksum="69AD31FB25897035" modified="2011-11-16" version="1" fragment="single">MSEKQSTDQYGRRKWNVELYEEEAKTKKETTSSSVQLSKQSIIQSETSSSEYIKHRNKLLQDSINAVKQFNLINPEVDVSKSFGANKRFGFFCPICDLSFRDNLALVDHLNSPQHVRKANEASRKLRKEGDQEESGELEGGIQRANLSEVISTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMI6</accession>
    <name>G3AMI6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_136705</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374945.1">
        <property type="nucleotide sequence ID" value="XM_007374883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMI6"/>
    <dbReference type="FunCoup" id="G3AMI6">
        <property type="interactions" value="276"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMI6"/>
    <dbReference type="GeneID" id="18869993"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136705"/>
    <dbReference type="eggNOG" id="KOG1991">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004196_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMI6"/>
    <dbReference type="OMA" id="MVMSMNK"/>
    <dbReference type="OrthoDB" id="760868at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013713">
        <property type="entry name" value="XPO2_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF28">
        <property type="entry name" value="IMPORTIN BETA SMX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08506">
        <property type="entry name" value="Cse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="7">
        <location>
            <begin position="24"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="997" mass="113714" checksum="F2B7DFF432150815" modified="2011-11-16" version="1">MDKGTLLKALGGTLDANPQVRKQSEAELHTFEQQPGFTAYLLDLIVEADIPLGIKISAAIFFKNRVVNYWLQPENKAPSPICIRDNEKGDIKEKLITTLFKSYKNTQIRIQLATALNSILSSEKWEELTLIIKDLLKDVHDVDRVYTALICVYEYTKNYRWAGLETGSNPVLEEITNEIFPLLESLTNKLLQSDDRIADEMLYLIIKTFKFTTYSAMPTYFQDMNKLGQWCQIHIVIINKPLPQEILDEDADQRTLHPRIKTIKWCFGNFHRLLSRHGGGVITKEKKTSQFAQNFLSNFVPEILSVYWKVIENWSTKTVWLSEASLFHLISFLEQLIETPAWDLISDKLDAIIRHVLMPTLSANEETIELYEDEPEEYIRRFFDINRESNTADVASINFIYRIASKKFRSTINLVLNIINEILSLRGNDRNNLEIAMKTEGAFRILSTVSYKLDVKLSPICGQVDKVLHTFVYPELLPESSAKTPWLTARACDTLAIFNTHKYTDMAILQDIFQGVVSCFSNDDQFPIQLTAADALCTLVEEDLVAEHVSKQAPQLMGNLLEKSKKFESDILTNVMDTFVQKFAKDLEPYAVELATQLVDQFMRLAAEILENTGGDIEDKEYQAASILSTLTTLIIAMIHSPKVGVSLEPVLENMIKVVLENAMVNFLTETIEILESLLFSSHDVSPTMWSIFQVVIESFETYAFEYFSFFQPFFEGIINYGFTKATMDSPYVQSFLNVCFNMLKSDDLDPLFAHSAFEDIELTILAMGPRFVPFLQAFLPEIFDIFSKLESENMFDGYMLHHLSILRVLFASIYIDPVTTVQFLTSKQFLASFYKLWLRHSDDFQSVYGCKLQILASLSLVNSQAITMIPEDLVGETVDLLLGNIAALPNAIKAKNLILAQESSSKQPQLGATGEDDEEDDYEGVDYEDDLEADEAELEAMKQTPIDEVNVFQEFTSAFLSMQQHDSRKHQVLFGGIEDSKKELIEHLINITREHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMK6</accession>
    <name>G3AMK6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">26S proteasome regulatory subunit 6B homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_60804</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374965.1">
        <property type="nucleotide sequence ID" value="XM_007374903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMK6"/>
    <dbReference type="FunCoup" id="G3AMK6">
        <property type="interactions" value="1315"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMK6"/>
    <dbReference type="GeneID" id="18873578"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60804"/>
    <dbReference type="eggNOG" id="KOG0727">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_2_0_1"/>
    <dbReference type="InParanoid" id="G3AMK6"/>
    <dbReference type="OMA" id="QDIGGMD"/>
    <dbReference type="OrthoDB" id="10255768at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000020">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000046">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000033">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="185"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="31"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW33450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="408" mass="45860" checksum="5E3A6C923CCDBCD0" modified="2011-11-16" version="1">MEDPIVLDSSINNALNKINHKQEEADVYLKLKKLEKELELLLLQEEYIKDEQRHLKRELVRAQEEVKRIKSVPLVIGQFLEPIDENTGIVSSTTGSNYVVRILSTLDRELLKPSSSVALHRHSNALVDILPPEADSSISIVGEDQKPDVTYADVGGLDMQKQEIREAVELPLTQGDLYAQIGIDPPRGVLLYGPPGTGKTMLVKAVANSTTASFIRINGSEFVQKYLGEGPRMVRDVFRLARENSPAIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFDQTSTVKVIMATNRHDTLDPALLRPGRLDRKIEFPSLKDRRERRLIFSTIASKMSLAPEVDLDSLIIRNDPLSGAVIAAIMQEAGLRAVRKNRYMILQSDLEEAYTSQVKTGTEHDKFDFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMW1</accession>
    <name>G3AMW1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_61452</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMW1"/>
    <dbReference type="GeneID" id="18873902"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61452"/>
    <dbReference type="eggNOG" id="ENOG502T60J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2392057_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMW1"/>
    <dbReference type="OrthoDB" id="4083131at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="94" mass="10545" checksum="C434B5152754E80D" modified="2011-11-16" version="1" fragment="single">MFKTQLTSYLFPQWFKSSKQEPVAATIPGPIPKSFTISNNDNKLINISMKEHTAEDEFIDISYKQLSYAEVAELAKNKKQKTKVAPVPNQPSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMY0</accession>
    <name>G3AMY0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bul1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_67014</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375670.1">
        <property type="nucleotide sequence ID" value="XM_007375608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMY0"/>
    <dbReference type="GeneID" id="18875130"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67014"/>
    <dbReference type="eggNOG" id="ENOG502T685">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_490030_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMY0"/>
    <dbReference type="OrthoDB" id="4016204at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039634">
        <property type="entry name" value="Bul1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022794">
        <property type="entry name" value="Bul1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007519">
        <property type="entry name" value="Bul1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904:SF1">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04426">
        <property type="entry name" value="Bul1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04425">
        <property type="entry name" value="Bul1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bul1 N-terminal" evidence="1">
        <location>
            <begin position="113"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Bul1 C-terminal" evidence="2">
        <location>
            <begin position="470"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="510" mass="58029" checksum="DDE815D1CADF7E68" modified="2011-11-16" version="1">MCAPAYDCKQFNRPDFQDPYYCDDDDDDDVQSILSIPEESNVLPSYYMYKSVVHNKFKYLSGPPSYSSLHDESSDLMQNSNPSTVSVATSISSTLQSLTSTNKISLEVRFDQPSNHDYKQGETISGHLILQNTCDSPINFSTIYILLEGSISFAETKAKTFLSMVDFDSVICPLLHIDANSIHKHPFTFRIPNYLLDSECAHVSNHLEIPPTTHYTMFCNAEVKYNLSGYILRQENCEYVNVGEIQKEVSIIPQYTNEEHINTNYESLLSKLLDETIYINKDRFNQIMCNLRITPLQNKQVITGNSKVSSVKFDANLLQGLKVKYVPAIPGHPDLNQTLSIPIEFKSTKGGEAPSIKSISAELVAATGKTYASCPVYIDDSMLFESFSNRKYNDIVVNPIIKMKGELYRLSHELDNSGLKKLLRFHIYYSNFAIPKVEYSNKEIKVNLKGMYRSLGDKSRTRNVYGGFNLTPNFQSCHMVRSYYLHLVVKFDWKSKNNVVINIPVKVGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN31</accession>
    <name>G3AN31_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calcium-binding protein NCS-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_61514</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the recoverin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375721.1">
        <property type="nucleotide sequence ID" value="XM_007375659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN31"/>
    <dbReference type="FunCoup" id="G3AN31">
        <property type="interactions" value="130"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN31"/>
    <dbReference type="GeneID" id="18873928"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61514"/>
    <dbReference type="eggNOG" id="KOG0044">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072366_1_2_1"/>
    <dbReference type="InParanoid" id="G3AN31"/>
    <dbReference type="OMA" id="EYVFNVF"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098744">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000009">
        <property type="entry name" value="Visinin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF178">
        <property type="entry name" value="NEUROCALCIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="96"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="190" mass="22165" checksum="F0E1D9DF1D449416" modified="2011-11-16" version="1">MGKAVSKLSKDELKSLRQATYFDKRELQQWYKGFLRDCPSGQLSEEEFVKVYKQFFPFGDPTDYCHYLFRVFDMDNSKYIDFKEFIIALSITSRGTEEQKLNWTFKLYDYKKAGSISYNDLLPIIEAIYKMIGPMVALPDDEKTPEARTEKFFTLLGKSKSDTINLSEFKQLSRLDPSILNSLNSYEGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN56</accession>
    <name>G3AN56_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Impact N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_152072</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IMPACT family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375174.1">
        <property type="nucleotide sequence ID" value="XM_007375112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN56"/>
    <dbReference type="FunCoup" id="G3AN56">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN56"/>
    <dbReference type="GeneID" id="18870916"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_152072"/>
    <dbReference type="eggNOG" id="KOG3299">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045276_2_2_1"/>
    <dbReference type="InParanoid" id="G3AN56"/>
    <dbReference type="OMA" id="SHPHMYA"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140469">
        <property type="term" value="P:GCN2-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.30">
        <property type="entry name" value="Impact, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023582">
        <property type="entry name" value="Impact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001498">
        <property type="entry name" value="Impact_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036956">
        <property type="entry name" value="Impact_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020569">
        <property type="entry name" value="UPF0029_Impact_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301:SF17">
        <property type="entry name" value="IMPACT FAMILY MEMBER YDL177C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301">
        <property type="entry name" value="IMPACT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01205">
        <property type="entry name" value="Impact_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00910">
        <property type="entry name" value="UPF0029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Impact N-terminal" evidence="2">
        <location>
            <begin position="24"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="143" mass="16238" checksum="F0DA37A1F8C719F8" modified="2011-11-16" version="1">MIRRTIRRLGTITNWHESEIIIDRKSKFQARNVNITSESEIPQVLSQFKQQHKSIAKNASHPHMLAWRTGEVLDGKQTNIKQGFKDNGESGAGSKILELLIKHNIVNVLVIVTRWYGGTPLGGSRFRHITNTTLKTLQNSNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANC5</accession>
    <name>G3ANC5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonucleases P/MRP subunit Pop8-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61567</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375784.1">
        <property type="nucleotide sequence ID" value="XM_007375722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANC5"/>
    <dbReference type="FunCoup" id="G3ANC5">
        <property type="interactions" value="106"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANC5"/>
    <dbReference type="GeneID" id="18873959"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61567"/>
    <dbReference type="eggNOG" id="ENOG502SCWV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2049401_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANC5"/>
    <dbReference type="OMA" id="NIDWITW"/>
    <dbReference type="OrthoDB" id="4077720at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000171">
        <property type="term" value="F:ribonuclease MRP activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034965">
        <property type="term" value="P:intronic box C/D snoRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000294">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, RNase MRP-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020347">
        <property type="entry name" value="Pop8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049128">
        <property type="entry name" value="Pop8-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28173">
        <property type="entry name" value="RIBONUCLEASES P/MRP PROTEIN SUBUNIT POP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28173:SF1">
        <property type="entry name" value="RIBONUCLEASES P_MRP PROTEIN SUBUNIT POP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20976">
        <property type="entry name" value="Pop8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribonucleases P/MRP subunit Pop8-like" evidence="1">
        <location>
            <begin position="15"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="126" mass="14353" checksum="FEC8BD7B67EEEDEC" modified="2011-11-16" version="1" fragment="single">MNDIAVTTEKADRIWYYLNLQIQPEPISDSNLVQAYNAVAIDLITWRLFISTALNKLYGLIGESTPYDIIATPTDKSIIIRTYYEDIDKFVTALMAYTFNLGKDTQVDINCYIKVSKRGEFIGLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANF9</accession>
    <name>G3ANF9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sphingomyelin phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_138608</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375224.1">
        <property type="nucleotide sequence ID" value="XM_007375162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANF9"/>
    <dbReference type="STRING" id="619300.G3ANF9"/>
    <dbReference type="GeneID" id="18870134"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138608"/>
    <dbReference type="eggNOG" id="KOG3770">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014743_2_0_1"/>
    <dbReference type="InParanoid" id="G3ANF9"/>
    <dbReference type="OMA" id="WIIAHLP"/>
    <dbReference type="OrthoDB" id="282973at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00842">
        <property type="entry name" value="MPP_ASMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041805">
        <property type="entry name" value="ASMase/PPN1_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340:SF27">
        <property type="entry name" value="ACL091CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442526" description="Sphingomyelin phosphodiesterase" evidence="3">
        <location>
            <begin position="18"/>
            <end position="747"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="747" mass="86205" checksum="276E230B5F6E320A" modified="2011-11-16" version="1">MLLVWLLVSLLLHSIHALEENHNNYYPPTELAVDDEEIVTRAIQELHNIDAASDLKTCDTCKTRLQVAKFLSLTRPDLVPQIFTTWCIEAGHDEIQCHMNYGYPNANYSSTGNDFTKMASLMSPSGADGDYFCYYHDKHCNILPETPIVDLSNWWPPKPRIYPAPEGNGDLFHVLHISDINLQLNYKMFAEANCSQSLCCTSRCSNDNFPPVVNDNLEGGYYDSSYSKNHFVKGNYQDITKLKKPVWSPARQFGEYTCDVPALLLNSTMQCIRDLHQNHLSFEFAIFTGGTVDHSDRSFVTKHKVLKSQDISHRILKHYLDSVPVIPTFGVRDIFPINQLPQKNLTENVNSYQWQFDFLSDLWSELGWIDFDATKQVRYSQIGFALTTLRGLKIISLNSNVWNVKNLYAFWDMLSLDSFGIWRFLVDELLQSEKQNQRVWILAHLPTNHQSLPLPTKIFAQIIERFSPKVIAAIFFGNIQVDTFMIHYAGDGTDTRQITNAINHALIGPSISPYAGMNPSWRYYAVDGLSFSVVNSFTYYTNLETTFHNDGAEPVWEFGYSARDIYDPDQIWPPESPLNTEWWHHVSEKIYESALMQATYEKFERRGRESDVVDVDTYCKLTSFTFDAKIQCMVGDDQDDFIEPQEANDYIPSLKPYEKVTYVEKEREDPEEEYNNKVEQVKQQPKYENVGSVVDAMEGQEFNNTEIEPLEEEIKFASQFRNNKGKSISLKDRIRNHNARITQLNRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANQ2</accession>
    <name>G3ANQ2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">STI1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_61098</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375263.1">
        <property type="nucleotide sequence ID" value="XM_007375201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANQ2"/>
    <dbReference type="FunCoup" id="G3ANQ2">
        <property type="interactions" value="1021"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANQ2"/>
    <dbReference type="GeneID" id="18873709"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61098"/>
    <dbReference type="eggNOG" id="KOG0548">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000134_46_1_1"/>
    <dbReference type="InParanoid" id="G3ANQ2"/>
    <dbReference type="OMA" id="WERREFI"/>
    <dbReference type="OrthoDB" id="2423701at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042030">
        <property type="term" value="F:ATPase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000010">
        <property type="entry name" value="Stress-induced phosphoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.100:FF:000002">
        <property type="entry name" value="Stress-induced-phosphoprotein 1 (Hsp70/Hsp90-organizing)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000027">
        <property type="entry name" value="stress-induced-phosphoprotein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041243">
        <property type="entry name" value="STI1/HOP_DP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006636">
        <property type="entry name" value="STI1_HS-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904:SF523">
        <property type="entry name" value="STRESS-INDUCED-PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904">
        <property type="entry name" value="TPR REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17830">
        <property type="entry name" value="STI1-HOP_DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00727">
        <property type="entry name" value="STI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="13"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="87"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="147"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="STI1" evidence="6">
        <location>
            <begin position="286"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="337" mass="37994" checksum="24548106594C640E" modified="2011-11-16" version="1">MEDAPQENHKEEADAAKAEGNALYKQRKFDEAIAAYEKAWNLNKDITYLNNRAAAEFEKGDYDAAIATCEKAIDEGRDMRADYTLIAKSFARLGNTYLKKDDLPNAVKYFDKSLTEHRTPDVLNKLRATQREIKVREANAYVDPEKAEAARLEGKEYFTKGDWPNAVKAYTEMIKRAPEDARGYSNRAAALAKLLSFPDAIQDCNLAIEKDPNFIRAYIRKATAQLAMREYSKCMETLNEARDKDSALGGKNIHEIDQLLNKAISSRFQAIEGETPEQTMERVSKDPEIVSILQDPVMQGILGQARDNPAALQEHMKNPDVNKKINMLIAAGVIRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3APA6</accession>
    <name>G3APA6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glucosamine-6-phosphate isomerase</fullName>
            <ecNumber evidence="4">3.5.99.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Glucosamine-6-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_61749</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>alpha-D-glucosamine 6-phosphate + H2O = beta-D-fructose 6-phosphate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:12172"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:75989"/>
            <dbReference type="EC" id="3.5.99.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.99.6" evidence="4"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375953.1">
        <property type="nucleotide sequence ID" value="XM_007375891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APA6"/>
    <dbReference type="STRING" id="619300.G3APA6"/>
    <dbReference type="GeneID" id="18874032"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61749"/>
    <dbReference type="eggNOG" id="KOG3148">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049611_0_1_1"/>
    <dbReference type="InParanoid" id="G3APA6"/>
    <dbReference type="OMA" id="MEFSKHI"/>
    <dbReference type="OrthoDB" id="7663298at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004342">
        <property type="term" value="F:glucosamine-6-phosphate deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006043">
        <property type="term" value="P:glucosamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006046">
        <property type="term" value="P:N-acetylglucosamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019262">
        <property type="term" value="P:N-acetylneuraminate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01399">
        <property type="entry name" value="GlcN6P_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004547">
        <property type="entry name" value="Glucosamine6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018321">
        <property type="entry name" value="Glucosamine6P_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00502">
        <property type="entry name" value="nagB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11280">
        <property type="entry name" value="GLUCOSAMINE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11280:SF5">
        <property type="entry name" value="GLUCOSAMINE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01161">
        <property type="entry name" value="GLC_GALNAC_ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="5">
        <location>
            <begin position="8"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW32677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="252" mass="27807" checksum="3EC306785481ED70" modified="2011-11-16" version="1">MRQATFSSAEDASEYLANYIISKVNNFQPTPSKPFVLGLPTGSSPEGVYARLIKANKAGKVTFKNVVTFNMDEYLGLKPTDKQSYHYFMYDKFFNHVDIPRENINILNGLATDIDKECANYEAKIKKYGRIHLFMGGLGPEGHLAFNEAGSSRDSITRRVSLVPSTIKANCRFFENDESKVPRYALSVGISTILDNSDEVALIVLGEAKAAALEKTIHGKKNDAQFPSSYLQDHPNALIVCDNAAAGLKSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3APF7</accession>
    <name>G3APF7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acyl-protein thioesterase 1</fullName>
            <ecNumber evidence="2">3.1.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyl-protein hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_61216</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Hydrolyzes fatty acids from S-acylated cysteine residues in proteins with a strong preference for palmitoylated G-alpha proteins over other acyl substrates. Mediates the deacylation of G-alpha proteins such as GPA1 in vivo, but has weak or no activity toward palmitoylated Ras proteins. Has weak lysophospholipase activity in vitro; however such activity may not exist in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="2"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375410.1">
        <property type="nucleotide sequence ID" value="XM_007375348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APF7"/>
    <dbReference type="FunCoup" id="G3APF7">
        <property type="interactions" value="492"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APF7"/>
    <dbReference type="GeneID" id="18873782"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61216"/>
    <dbReference type="eggNOG" id="KOG2112">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049413_3_8_1"/>
    <dbReference type="InParanoid" id="G3APF7"/>
    <dbReference type="OMA" id="WYDILAM"/>
    <dbReference type="OrthoDB" id="2418081at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050565">
        <property type="entry name" value="LYPA1-2/EST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655:SF17">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="Phospholipase/carboxylesterase/thioesterase" evidence="10">
        <location>
            <begin position="8"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="233" mass="25603" checksum="A79A56DD22FF406B" modified="2011-11-16" version="1">MSNLINAIRVSSQKTPVKGAVIFLHGLGDTGEGWSWFPQLINQTKIIKNSDAINYVFPNAPQIPITVNGGYVMPAWFDIYAFGDPNARQDVTGFFKSCEVLKSLIKEQIEVHGVPPEKIIIGGFSQGAAISLATASILDFKIGGVVALSGFCPVKNDVLERHEKSGVNFNTPIFQGHGKADPLIKFDYGQQTSEFYKSLGFNNYTFRGYEGVAHSADDQELVDVMKFIHDIVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3APF8</accession>
    <name>G3APF8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_152629</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DOP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375411.1">
        <property type="nucleotide sequence ID" value="XM_007375349.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3APF8">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APF8"/>
    <dbReference type="GeneID" id="18870933"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_152629"/>
    <dbReference type="eggNOG" id="KOG3613">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001197_1_0_1"/>
    <dbReference type="InParanoid" id="G3APF8"/>
    <dbReference type="OMA" id="GLETCIA"/>
    <dbReference type="OrthoDB" id="297643at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040314">
        <property type="entry name" value="DOP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056457">
        <property type="entry name" value="DOP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007249">
        <property type="entry name" value="DOP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056458">
        <property type="entry name" value="TPR_DOP1_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042">
        <property type="entry name" value="DOPEY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042:SF24">
        <property type="entry name" value="PROTEIN DOPEY-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24598">
        <property type="entry name" value="DOP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04118">
        <property type="entry name" value="Dopey_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24597">
        <property type="entry name" value="TPR_DOP1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DOP1 N-terminal" evidence="7">
        <location>
            <begin position="26"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like middle TPR" evidence="8">
        <location>
            <begin position="351"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like C-terminal" evidence="9">
        <location>
            <begin position="1217"/>
            <end position="1664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW32135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1686" mass="192503" checksum="7649F478D998A31B" modified="2011-11-16" version="1">MDSPKGPNSVLKLVTSRKSKTHQLSSKDKKYLQSVEKALGSFDALEEWADYIAFLSRLQKSLQFTPDPSVQEQYYVPLSSQVSNKLSLCLSSRLPSGVHQKALSIYEFIFENLPIETLNEDISVWLPGLLPLFSYSSISIKPQQIRIFKDHIVAKLDVDHLRNISKPFILSLLSGLDDENSEAFNDILQLTEEFKEKLNNDSHFWQSMFLCIISNPERRLGALHWCNRNLPRFTESKGNHELSEEAKMCLKPEPGLLIRAFAVTINPPVTIDSASDIIVVRGFFDLLLSHIPLESSIFDSQVYPKDKEMLLMSCCRVTLKKDMSLNRRLWNYLLGPDTEGDHQKRIPRTEYFAKYALETLSSGLLKMVESDSTKSKIEAFKISLSIIMDKWEISTVITPRLFSRFLNIAFENKESKDLMVSASSFFDGIESTYIWNDILQLVAEEDESKLEILDFVLKEFNFNEEEMITLHAPLAIICLLGKSDINVKRLEILDTLFNLVPARAYGIADQKVNDTYIDIVSKIKGFYRGRLNNDNPQVPFSANEVSFLVIDMLQKKFIQYMSNTAYCFKISNLLVQMLNIVPRDETSLELDSELVNKMLELQVPEYIEESDRNQEGLLISYAIAKSFGNISKSMSDAQKEKTLKAIISNLWTSIYSSEPANYQVEAVKTIYEMGTSYPFHSIEAGVVELFLKLPINKRITALETLWTHSASVNDGDTILERPLQILLDDISETDKKSNLGIHDFLRRIIKNGGANRLLKLLSNPILNFTFMHGERKTLTIDDDLGQFAYYLGLIIKVIESNTKLLRESCSNEFAAMDNSNKLNLLRSNEWDISTYKSLFLSIIEKFFHLKLTDEVLNDEYSLDNYYSSMEHCLKILTLLVTGNEKSFNELFNLLIGTCTVYLGLNVASYTIELVESQFLKCLFHFLELAQELHLPLNLLNVEEEKNEPVLVTFLIQGITNSKTSILLENWITLLTRSLYLLGDSIFNVIITLNRTIIAKLEGIFKNFSSFEKFSELEDLNSCVNILVTGLEDLLSITHSYLLTSSIRSNAEKGPSGAAENGFLNNMISGVFQIESPELRTTEQNKRYSIILCFQEALRAGFHIWYWCDSRPKVPDGAKYYSDRSLTYISHKLKFRAKKLLEALIELERQEVIESLIDLNGASPGALKLLNILDGGRSQVTLPHLFNSIISRCYPQMLDQAQRSSMYTQIPTTELSSFLLSFFDAIDNDTVIDVWNLSINFFKNILLHPGSFKDVYPDSLRICKVLSMKLKSSKVRDQKNRKELADVFTRLFQNAVTKAHSDPREEIELESKKDSSSVQSSTVQQEDVIEAITEIIPFLDEICQDTDKVTQSLNTLINSFILPQIKSKKIPNINLRVLGLLEIVGKHHPNKTWKTLVLDLFMDNSFFQTDFDKLTIWNSILGAWITNDREKLGDMISRITTSSGSSPSNLFVWNEASELEAKVFILKRISYLILIQPIDFFLNFLDSIFDKIEALLSGNCPEIIRAEISVLFRAIVLKFNELHLLPRWATVSHELLSIFESIEEKSTKELHALSKESLKLILNGCKLLDQLLLCSYDEFNLNEWLFISTDTEFFNGPLDNPTGSIIDQISRKHDLTYLKDSPLSLEQPQDNLHPLLEGVREIDSITKLRLFFDSLSLIQYERTYGLFNVEIDSCIADVLGDLMNPVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3API8</accession>
    <name>G3API8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sm protein F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_61242</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the snRNP Sm proteins family. SmF/LSm6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375435.1">
        <property type="nucleotide sequence ID" value="XM_007375373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3API8"/>
    <dbReference type="FunCoup" id="G3API8">
        <property type="interactions" value="734"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3API8"/>
    <dbReference type="GeneID" id="18873795"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61242"/>
    <dbReference type="eggNOG" id="KOG3482">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_12_0_1"/>
    <dbReference type="InParanoid" id="G3API8"/>
    <dbReference type="OMA" id="DNYMNFQ"/>
    <dbReference type="OrthoDB" id="409625at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034715">
        <property type="term" value="C:pICln-Sm protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008266">
        <property type="term" value="F:poly(U) RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990935">
        <property type="term" value="F:splicing factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036261">
        <property type="term" value="P:7-methylguanosine cap hypermethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01722">
        <property type="entry name" value="Sm_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000070">
        <property type="entry name" value="Small nuclear ribonucleoprotein F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016487">
        <property type="entry name" value="Lsm6/sSmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034100">
        <property type="entry name" value="Sm_F"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F SNRNP-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="8"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="86"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="97"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="117" mass="13526" checksum="95D9E8B4B264EB9F" modified="2011-11-16" version="1">MSTFQPINPKPFLKSLIGKQVIVRLKWNKTEYRGTLISIDNYMNLQLDQTYEIIYEVEDGNETKKEESIGEIFIRCNNVLFVREAKSEPQETEIETETSVATESNGNENGNKEMETD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3APV2</accession>
    <name>G3APV2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61359</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375549.1">
        <property type="nucleotide sequence ID" value="XM_007375487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APV2"/>
    <dbReference type="GeneID" id="18873856"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61359"/>
    <dbReference type="eggNOG" id="ENOG502QZYD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1086520_0_0_1"/>
    <dbReference type="InParanoid" id="G3APV2"/>
    <dbReference type="OMA" id="GQYSERY"/>
    <dbReference type="OrthoDB" id="4026805at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="202"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="256" mass="28959" checksum="CD3D294AA36EDDB2" modified="2011-11-16" version="1">MLVTFKYNRDKYNQLLEQSVSNTSPYVKEETPSVSGSTPPVSTPTKFVTLHYNSGYMTSPIKSQRRSRNRTPTPPSLTPNTTLPFQKSFSVIDSSPIKISKEDTADMQEQCSYQDAIESMATYNEQYILNPRNNDDIPSVIAHNQQVEKAWFNNIRSLADRLHLLRETYRTVQMLKKSKVNRNGLVDESAYSKSTTPRIEDSPSVSVPPTTPSSRLVPETTSGVDEDEYEEIDGFEEDEDSSNTDTVTPKRSEQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ11</accession>
    <name>G3AQ11_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial group I intron splicing factor CCM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_66958</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375608.1">
        <property type="nucleotide sequence ID" value="XM_007375546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ11"/>
    <dbReference type="STRING" id="619300.G3AQ11"/>
    <dbReference type="GeneID" id="18875128"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66958"/>
    <dbReference type="eggNOG" id="ENOG502RPYD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_417961_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQ11"/>
    <dbReference type="OMA" id="ATRHDQF"/>
    <dbReference type="OrthoDB" id="4017550at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="548" mass="64329" checksum="B3D6DA02E9BDC22E" modified="2011-11-16" version="1">MNKNSVKMTKEYGKFIKELYPRTKKSDPIFRDVTKEFNVVNNSIKYSSIVFHERLFQAYMSLPSPRALFITSDDLNQLVQVFLYASRNFHRLNTETNIKQKPTLAMRKYAKRKLDQRKAYSNMCSKILQDIVEAKLPLTPTERIKMVQLSLFKDRQNIAKYYDQEVQHSHYEPLKYRKFNYETYTQLRKSLPPGTNSNLLLLDLALRNNEMEVVEEIWNSNEYVSGSGVIMLLNYFQTKLNKPDTIDYLIKILHEINSMKSVKAAVIDEVISLLTKMGRINLAQDLVQAAYYSKLDPKKSWVHYSERDYYAWCRSIYHRLMAITKDYEVIAELHPSIKTFLSLAEYHCYTPSSSFSQVRDVLRLMDKTTDIPTSQVFYKLILKGFDIHSKIKDRQDWTIDNLLQITSEIIGEVNTVNSHEAIMDLIKSGDIESLQQLEPNFHVAFKEEETKGEVKTSISADFLDIILDIYVVTLESYYRATEDDRYNDAIMEIKRIKEQHTRKYSIAMHTTHEYSRIIYDETETKKAYLIEAINRVSDIISREDGLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ72</accession>
    <name>G3AQ72_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">R3H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_51436</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376197.1">
        <property type="nucleotide sequence ID" value="XM_007376135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ72"/>
    <dbReference type="GeneID" id="18871557"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51436"/>
    <dbReference type="eggNOG" id="KOG2953">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_676137_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQ72"/>
    <dbReference type="OMA" id="ALYMKLR"/>
    <dbReference type="OrthoDB" id="278430at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02642">
        <property type="entry name" value="R3H_encore_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="R3H" evidence="2">
        <location>
            <begin position="47"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="380"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="403" mass="46777" checksum="147E96FFCF3EEB4C" modified="2011-11-16" version="1">MTIQPNTLAQHPKQHKQQPQRQQTNQVEQPHVIPTSIQTALSKQQDRIYLLHLEQELIKFIESVSKDLTASYAIPASYLKNSYYRLLSHQLCQYYKLSHWNNKATNEITVTMVEGVDYTFINTSIFVRLNKAVAEVEEQQQQQQQQQQQQQQQQQQQQEQTDATEPPIQPLMKPKLIVKKKKEVSVPTSATSENSETSRDNSFIQDTASSTPTSINESVNNIESERASKEALYMKLRQEIFQNEDDQEEEEEEEEQEEDEDRDIDNQSFSRSPFNNNNIYGQQIPMQMYPNGYYPTPIVYPGYSAYPQYPQYVTPMPTTSNIPATPPYPNFPMYNPSLVQPSAPLPYDKETERRLLNNPYIIIPDNNHRQKSGHKRYYKKNNTNGYSKGTNGQTNARSSTNNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQA0</accession>
    <name>G3AQA0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Acyl-coenzyme A oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">SPAPADRAFT_154610</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2,3-saturated acyl-CoA + O2 = a (2E)-enoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:38959"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="ChEBI" id="CHEBI:65111"/>
            <dbReference type="EC" id="1.3.3.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; peroxisomal fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the acyl-CoA oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376225.1">
        <property type="nucleotide sequence ID" value="XM_007376163.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQA0"/>
    <dbReference type="FunCoup" id="G3AQA0">
        <property type="interactions" value="408"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQA0"/>
    <dbReference type="GeneID" id="18870991"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_154610"/>
    <dbReference type="eggNOG" id="KOG0136">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014629_3_1_1"/>
    <dbReference type="InParanoid" id="G3AQA0"/>
    <dbReference type="OMA" id="SINKRFA"/>
    <dbReference type="OrthoDB" id="538336at2759"/>
    <dbReference type="UniPathway" id="UPA00661"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003997">
        <property type="term" value="F:acyl-CoA oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033540">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA oxidase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000018">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000015">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000003">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055060">
        <property type="entry name" value="ACOX_C_alpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029320">
        <property type="entry name" value="Acyl-CoA_ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012258">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002655">
        <property type="entry name" value="Acyl-CoA_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909:SF352">
        <property type="entry name" value="ACYL-COENZYME A OXIDASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909">
        <property type="entry name" value="ELECTRON TRANSPORT OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01756">
        <property type="entry name" value="ACOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22924">
        <property type="entry name" value="ACOX_C_alpha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14749">
        <property type="entry name" value="Acyl-CoA_ox_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000168">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12 14" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 12" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-coenzyme A oxidase N-terminal" evidence="18">
        <location>
            <begin position="31"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="17">
        <location>
            <begin position="154"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-alpha1" evidence="19">
        <location>
            <begin position="293"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-terminal" evidence="16">
        <location>
            <begin position="515"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="456"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF14749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF22924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EGW31447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="709" mass="78820" checksum="F2F2B788A68E6BF3" modified="2011-11-16" version="1">MAIATNTVNISQGPDPKTSIQNERANSKFDPVKMHYFLEGSKERAEELKSLMQQMERDPVLFTDASYYDLTKDQQRALTAVKINRVSRYLEGDSIDTFNKRLSLIGLFDPQVATRIGVNLGLFISCVRGNGTYEQLKYWALDKETAHIKGIYGCFGMTELAHGSNVSGLETTATFDKDTDEFVINTPHIGATKWWIGGAAHSATHTTCYARLIVDGEDYGVKTFVVPLRDSNHDLMPGISVGDIGSKMGRDGIDNGWIQFSNVRIPRFFMLQKFCKVSREGKVILPPLEQLSYSALLGGRVMMVLDSYRMIARMSTIALRYAIGRRQFKGDNVDPDDENALETQLLDYPLHQKRLLPYLAAAYVFATGAVRIEQTIDETLSDLDDAVDQNDDEAIMKSIDAMKSLFIDSGSLKSTCTWLAAEAIDQCRQACGGHGYSAYSGFGKAYNDFVVQCTWEGDNNILGVNIGKPLVKHVLAIEDDGKVVKGSSSFLNNLKQYTGSNATNVILDNVADLNDLKKVAKAVEIAIIRLAHQAATTARKNSLDYVGAELVTLSKLKAHHYLLNEYVVRLERFEEKDLIPYLVSLGRLYAATMVLERFAGIFLTFNVVSPSVMAALTSNQIPQLCKEVRPHVVALTDSFQQSDMLVNSVLGKYDGDIYENYFGTVKTLNKPSNTKAPYSGALEAMLNRPSIEERERFEKTVETGEILSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQM7</accession>
    <name>G3AQM7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_62190</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376352.1">
        <property type="nucleotide sequence ID" value="XM_007376290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQM7"/>
    <dbReference type="GeneID" id="18874210"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62190"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_3_1"/>
    <dbReference type="InParanoid" id="G3AQM7"/>
    <dbReference type="OMA" id="NSFENRE"/>
    <dbReference type="OrthoDB" id="648285at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF73">
        <property type="entry name" value="METABOLITE TRANSPORT PROTEIN YDL199C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="258"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="353" mass="40184" checksum="79E9A74D773ACD1E" modified="2011-11-16" version="1">MAHLSWRLPLFMQVGLAVVLFCGGFFIVESPRWLLDNDMDQQGFNVLALLYDSDPTITKPKSEFFMIKNSILQERITSPKSERSWKKLFTNHLLRVAIACSAMGFAQLNGINIISYYAPMVFKEAGFNDANAILMTGINSLIYLMSTIAPWFLVDRWGRKPILISGGAAMGICLYMVALIMYLNVSATPSLVALLVIIYNAGFGYSWGPIGFLIPPEAYPLSVRAKGVSLAVSTNWLCNFVVGLLAPILKQDIGWKMYLYPATSCIISIFIVLLFYPETKGVELEDMDKVFTDFYKDTTLVRVSKMRLRKRRMNKNDYNQNFNQPNSFENREGLNEIELEDINDLDYERRQML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQN7</accession>
    <name>G3AQN7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Fatty acid synthase subunit alpha</fullName>
            <ecNumber evidence="19">2.3.1.86</ecNumber>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="19">3-oxoacyl-[acyl-carrier-protein] reductase</fullName>
                <ecNumber evidence="19">1.1.1.100</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="19">3-oxoacyl-[acyl-carrier-protein] synthase</fullName>
                <ecNumber evidence="19">2.3.1.41</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="27" type="primary">FAS2</name>
        <name evidence="27" type="ORF">SPAPADRAFT_140888</name>
    </gene>
    <organism evidence="28">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. The alpha subunit contains domains for: acyl carrier protein, 3-oxoacyl-[acyl-carrier-protein] reductase, and 3-oxoacyl-[acyl-carrier-protein] synthase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 19">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 19">
            <text>a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>acetyl-CoA + n malonyl-CoA + 2n NADPH + 4n H(+) = a long-chain-acyl-CoA + n CoA + n CO2 + 2n NADP(+).</text>
            <dbReference type="EC" id="2.3.1.86"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 19">Belongs to the thiolase-like superfamily. Fungal fatty acid synthetase subunit alpha family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.86" evidence="19"/>
    <dbReference type="EC" id="1.1.1.100" evidence="19"/>
    <dbReference type="EC" id="2.3.1.41" evidence="19"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376362.1">
        <property type="nucleotide sequence ID" value="XM_007376300.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AQN7">
        <property type="interactions" value="360"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQN7"/>
    <dbReference type="GeneID" id="18870327"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140888"/>
    <dbReference type="eggNOG" id="ENOG502QQJX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000114_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQN7"/>
    <dbReference type="OMA" id="FPTLPDW"/>
    <dbReference type="OrthoDB" id="4251012at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005835">
        <property type="term" value="C:fatty acid synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004321">
        <property type="term" value="F:fatty-acyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101026">
        <property type="term" value="P:mitotic nuclear membrane biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900535">
        <property type="term" value="P:palmitic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00828">
        <property type="entry name" value="elong_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08950">
        <property type="entry name" value="KR_fFAS_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.20:FF:000005">
        <property type="entry name" value="Fatty acid synthase alpha subunit FasA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2490:FF:000001">
        <property type="entry name" value="Fatty acid synthase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.25.70:FF:000001">
        <property type="entry name" value="Fatty acid synthase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="entry name" value="4'-phosphopantetheinyl transferase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00101">
        <property type="entry name" value="AcpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002582">
        <property type="entry name" value="ACPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040899">
        <property type="entry name" value="Fas_alpha_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047224">
        <property type="entry name" value="FAS_alpha_su_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026025">
        <property type="entry name" value="FAS_alpha_yeast"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041550">
        <property type="entry name" value="FASI_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050830">
        <property type="entry name" value="Fungal_FAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004568">
        <property type="entry name" value="Ppantetheine-prot_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00556">
        <property type="entry name" value="pantethn_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982:SF21">
        <property type="entry name" value="FATTY ACID SYNTHASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982">
        <property type="entry name" value="MALONYL COA-ACYL CARRIER PROTEIN TRANSACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18325">
        <property type="entry name" value="Fas_alpha_ACP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18314">
        <property type="entry name" value="FAS_I_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000454">
        <property type="entry name" value="FAS_yeast_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="4'-phosphopantetheinyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 22" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="10 27" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 19 23" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carrier" evidence="25">
        <location>
            <begin position="142"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="26">
        <location>
            <begin position="1116"/>
            <end position="1656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="588"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1350"/>
            <end position="1369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="24">
        <location>
            <begin position="597"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="1350"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="active site" description="For beta-ketoacyl synthase activity" evidence="20">
        <location>
            <position position="1299"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="1767"/>
            <end position="1769"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="1767"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="1768"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="1769"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1793"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1803"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="1812"/>
            <end position="1828"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="1836"/>
            <end position="1839"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="1866"/>
            <end position="1868"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="1867"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="1868"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="23">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00060261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="EGW31584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1881" mass="206444" checksum="D991B8A07483FFC7" modified="2011-11-16" version="1">MRPEIEQELSHTLLTELLAYQFASPVRWIETQDVFLKQHNTERVVEIGPSPTLAGMASRTIKAKYESYDAALSLQRQVLCYSKDAKEIYYTPDPADIAPPAAAAAESAPAAEAAAPVAAAPVAAAPAAAAAGPAASIPDEPVKASLLLHVLVAQKLKKPLEAVPMGKAIKDLVNGKSTVQNEILGDLGKEFGSTPEKPEDTPLEELAEQFQDTFNGQLGKTSTSLIGRLMSSKMPGGFSITVARKYLETRFGLGAGRQDSVLLVALTNEPANRLASEAEAKAFLDSVANKYASTAGISLSSAAPAGGAGGAAGGAVVDSAALDALTAENKNLARQQLEVLARYLQVDLNKGAKSFIKEKEASAVLQKELDLWEAEHGEFYASGIKPTFSPLKSRTYDSYWNWARQDVLSMYFDIIFGKLTSVDRETINQCIQIMNRANPTLIKFMQYHIDHCPEYKGETYNLAKRLGQLLIDNCQQVLNSDPVYKDVSRITGPKTTVCAKGNINYEEANKESVRKFEQYVYEMAQGGKMTKIEQPTIQEDLARVYKAITKQASKSNKLELQKVYDQLLKVVESSTEIETQQLTKDVLQSTATSTTVSEDELSTASDDEDDEIASLPDRTSILQPVSSTIPAQTIPFLHIQRKSKTGWEYDRKLSATYLDGLESAAVNGLTFKDKYVLLTGAGVGSIGAEILQGLISGGAKVVVTTSRFSKKVTEYYQNMYARYGAAGSILTVVPFNQGSKQDVEALVQYIYDEPKKGGLGWDLDVIIPFAAIPENGNGIDNIDSKSEFAHRIMLTNLLRLLGAVKARKTTDTRPAQCILPLSPNHGTFGFDGLYSESKISLETLFNRWYSEDWGTKLTICGAIIGWTRGTGLMSGNNIIAEGIEKLGVRTFSQKEMAFNILGLLTPEIVKLCQEEPVMADLNGGLQFIENLKEFTSKLRTDLTENADIRRAVAIESSIEQKVINGDNVDANYSKVTVQPRANMKFDFPTLKTYDQIKAVAPELEGMLDLESVIVVTGFAEVGPWGNARTRWEMESKGEFSLEGAIEMAWIMGLIKYHNGNLKGKPYSGWVDAKTQTPVDDKEIKAKYEEEILDHSGIRLIEPELFHGYDPKKKQMIQEIVVQHDLEPFECSKETAEQYKHEHGDKCEIFEIEDSGEYTVRILKGATLFIPKALRFDRLVAGQIPTGWDARTYGIPEDTINQVDPITLYVLVATVEALLSAGITDPYEFYKYVHVSEVGNCSGSGMGGVSALRGMFKDRYADRPVQNDILQESFINTMAAWVNMLLLSSSGPIKTPVGACATAVESVDIGVETILSGKAKIVIVGGYDDFQEEGSYEFANMNATSNAVEEFKHGRTPKEMSRPTTTTRNGFMEAQGSGIQVIMTADLAIKMGVPIHAVLAMTATATDKIGRSVPAPGKGILTTAREHHGDLKYPSPLLNIKYRKRQLAKRLEQIKAWEEGEINYLQDEAELTKEEYGDEFSIKDFMRERTEEIHREAKRQIADAKKQWGNHFYKSDPRIAPLRGALATFNLTIDDIGVASFHGTSTVANDKNESATINSMMQHLGRSEGNPVFGVFQKYLTGHPKGAAGAWMLNGGIQILESGIVPGNRNADNVDKLLEQFEYVLYPSRSIQTDGIKAVSVTSFGFGQKGAQAIVIHPDYLYAVLDQSTYHEYAAKVTARNKKTYRYMHNAITRNTMFVAKDKAPYSDELEQPVYLDPLARVESSKQQQLVFSNKSIQNNKSYVSEVADKTAKALTSLTKSSKGVGVDVELLSAINMENETFIERNFTDAEREYCQKSSSPQASFTGTWSAKEAVFKALGVESKGAGASLIDIEIIRDANGAPKVFLNGEAKVAATKAGVKNVNVSISHDDFQATAVALSEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQQ0</accession>
    <name>G3AQQ0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_51591</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376375.1">
        <property type="nucleotide sequence ID" value="XM_007376313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQQ0"/>
    <dbReference type="STRING" id="619300.G3AQQ0"/>
    <dbReference type="GeneID" id="18871573"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51591"/>
    <dbReference type="eggNOG" id="ENOG502RQ2R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_702420_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQQ0"/>
    <dbReference type="OMA" id="YYSTHRE"/>
    <dbReference type="OrthoDB" id="4012911at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="136"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="236"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="312"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="393" mass="45560" checksum="AF14511359785807" modified="2011-11-16" version="1">MEESNSSIQSTSSKSSSKKTNFQFTYDADNYLLDLIYRNREDIFTRGNKLRTWKHILTQFNSRFDSGIVQTRTISHRFQNLIANLEKKLIHVPSMLEPGLLNENETILMEIVELMIKTNSGISFTLADLVKKVYRPTESHSHDSGTNSEVRISPTPNPRAAPTPVPTTIHQNHFNTINGYEEQLRLAETANNYVQLKSQKPLQQQHNNAQIPDQPMLIRGKSHEFHGCRDQYIPDPSIRPPEHNHNNIQVLESSSSYKDRSSSNGSIERNMYYSTHREEPHVQHSSSTGRNPDPRSMMSHDQEIFSKFLLLRNEYNAQMNNIKLEIRQLRNEIDFKVKKIEDSIAVSNDSMDEKLANFYELLMAKRDWNHRIDRMSEPNENRNIEQNFGATSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQT7</accession>
    <name>G3AQT7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA polymerase</fullName>
            <ecNumber evidence="12">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_51626</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="12"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376412.1">
        <property type="nucleotide sequence ID" value="XM_007376350.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AQT7">
        <property type="interactions" value="954"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQT7"/>
    <dbReference type="GeneID" id="18871578"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51626"/>
    <dbReference type="eggNOG" id="KOG0970">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001718_1_0_1"/>
    <dbReference type="InParanoid" id="G3AQT7"/>
    <dbReference type="OMA" id="MTKMNVG"/>
    <dbReference type="OrthoDB" id="6755010at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000510">
        <property type="term" value="F:H3-H4 histone complex chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000731">
        <property type="term" value="P:DNA synthesis involved in DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006278">
        <property type="term" value="P:RNA-templated DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05776">
        <property type="entry name" value="DNA_polB_alpha_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05532">
        <property type="entry name" value="POLBc_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000004">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000003">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3200.20:FF:000002">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000036">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2820:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3200.20">
        <property type="entry name" value="DNA Polymerase alpha, zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024647">
        <property type="entry name" value="DNA_pol_a_cat_su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038256">
        <property type="entry name" value="Pol_alpha_znc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045846">
        <property type="entry name" value="POLBc_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015088">
        <property type="entry name" value="Znf_DNA-dir_DNA_pol_B_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00592">
        <property type="entry name" value="pol2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45861">
        <property type="entry name" value="DNA POLYMERASE ALPHA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45861:SF1">
        <property type="entry name" value="DNA POLYMERASE ALPHA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12254">
        <property type="entry name" value="DNA_pol_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08996">
        <property type="entry name" value="zf-DNA_Pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 12" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase alpha catalytic subunit N-terminal" evidence="17">
        <location>
            <begin position="11"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="15">
        <location>
            <begin position="435"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="14">
        <location>
            <begin position="749"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger DNA-directed DNA polymerase family B alpha" evidence="16">
        <location>
            <begin position="1238"/>
            <end position="1440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="46"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="156"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="162"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="233"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW31634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1445" mass="164331" checksum="A87E246438AC8B0B" modified="2011-11-16" version="1">MSSRAAKLQKLKELREARKTGRTITTYSDDDDHDGAIYDEVDEETYRQHKRDQLMNDDFVVDDGGEGYVDNGADEWDDASRPNYYSDEEDQGPTKKRKKQKVVKVSKSAQINNYFKPTTRVNATTKIDTNIDDILDDFQEVTSKKKKFTTPFSTIKKGSPKVKSTASPFDFSTTGSRRKRQIVEEFNASSDYTVDEPEPEPEQQPSSPIKMVKSETPETITKPILEEVKSEDITMEDDDDDSEDDVVYSKRPRASATHKHTIKTVSSVKADEFTSSPTTLFANHTDKLDESIIMDTESTDSFKMFWLDYCEVDNTLLLFGKILTKDGKLASGVVQVNGLCRELYFLPRKYRMENGEENEDEPVGPLDVLDELTPILLEQYNLQELKAKPETMKYAFELAEIPKETEYLKVLLPFKTPKNQHMIMPANIEGETFNHVFGTNQNIFEAFVTQRNVMGPCWLTIEGGDFHSIQNTSHCQVEVAVDSPSQISVISGVNTPQPPNLTITSISVQTMMNPKENKQEIVSVSLATYYEVPQDAPIDENLQPNDIITLVRPVQSSLVPVGLQQLANKTGLKLRICPNEKVLLNCLAAMVKSHDPDVFIGHRLENISLDVLVHRMHAHNVTTWSAFGRRNRKRWPHSINKSGSGFSNNLLIREIFAGRLLCDIANEMGQSLTMKCQSWDLHEMFQVVLGKTHAPLEINFMQPGYSEDSHMLLMALKENEFNVKVTAEIAFAIQILSLSKQLTNIAGNAWSHTLSGTRAGRNEYILVHEFKRNNYIVPDKQDQTHKNTSYNQEARLENIDDDATTATSNKKPKYQGGLVFEPEKGLHKNYILVMDFNSLYPSIIQEFNICFTTVDRDRFNVSHDEDRDLPVLPERESEPGVLPRLLNTLVSRRREVKKLLKDPKNTPFQRAQYDIKQQALKLTANSMYGCLGYVNSRFYAKPLAMLVTNKGREILMDTRQLAESSGLRVVYGDTDSVMIDTGADNFQHALKVGEEFKIQVNERYRLLEIDIDNVFKRLLLHAKKKYAAMNVSIKNGVESTSLEVKGLDMRRREYCQLSKDISTFVLMKILSNMDPEQALSEIYDYLEDQKNKIISNEILMDKFKINTRLSKDPANYPNGKSMPQVQVALRLRKQGKVIKAGNVVTYVITSPADDSDKSTASERARSIQEVITNKDKYKPDAMYYLEKQIFAPVERLLERIEGVDMMRVATSLGIDTKKYILRVRNSDGPNGEIAPLESNISDSERFRQSSYLVLKCKCGHSFRFGGIVASKDYKVSFNGVTCLKCDHTFSVLKLTAQLEQAIRHHIALYYSGWLICDDPACGIMTRQISVYGRRCIGSSGKAMDCKGVMRYRYSDKALYNQLLYFDSIFNIDKAKKNQLRPIEEEEEDNDNGEKKQAPLAQGQVNALAEQNSEMFGYCQAVVQKYLAECGRRYVNMGSIFEFMAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARB1</accession>
    <name>G3ARB1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein OCH1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">OCH1</name>
        <name evidence="3" type="ORF">SPAPADRAFT_155280</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376496.1">
        <property type="nucleotide sequence ID" value="XM_007376434.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARB1"/>
    <dbReference type="STRING" id="619300.G3ARB1"/>
    <dbReference type="GeneID" id="18871016"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_155280"/>
    <dbReference type="eggNOG" id="ENOG502QW2I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022381_5_0_1"/>
    <dbReference type="InParanoid" id="G3ARB1"/>
    <dbReference type="OMA" id="WVFAARP"/>
    <dbReference type="OrthoDB" id="409543at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039367">
        <property type="entry name" value="Och1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834:SF1">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="372" mass="42997" checksum="27D60481FF28DAEE" modified="2011-11-16" version="1">MIFAENLSAGSTEAPPEPENFLTKSPKAPPKSFPGHIYPKEQFLKELHDEPFYKVTGFNLQPINPGKVPYNTTVFQQLQAKVPYDPTVSIPKMVWQMWKVPLDHEEFPGDYRDYHQTWLDKASDYEHIFKTNDEYDAVVNELYADIPEVLHAYNILPQTILKCDFSRYLLLYAYGGIYADLDTILWKPIDTWITNQKSYLHRPLDPGIIVGIENDEGARINNWTILSKKGHPMLAELINQITEHTLWREETGQLKAIFRNPRDNVIDWTGPGRFTDMTYKYLNNILQTDFDRFETIIEYPLLEYIRNPFVLGDIMVLPRSSMRAEYNPENDPLAYISHNFAGAWKADQDKEEEAENTAVVAAAAAAENTEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARB9</accession>
    <name>G3ARB9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Mediator of RNA polymerase II transcription subunit 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Mediator complex subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_140080</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 11">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Mediator complex subunit 6 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376505.1">
        <property type="nucleotide sequence ID" value="XM_007376443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARB9"/>
    <dbReference type="FunCoup" id="G3ARB9">
        <property type="interactions" value="823"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARB9"/>
    <dbReference type="GeneID" id="18870267"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140080"/>
    <dbReference type="eggNOG" id="KOG3169">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077754_0_1_1"/>
    <dbReference type="InParanoid" id="G3ARB9"/>
    <dbReference type="OMA" id="MQRQFSQ"/>
    <dbReference type="OrthoDB" id="344220at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.580:FF:000004">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.580">
        <property type="entry name" value="Mediator complex, subunit Med6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007018">
        <property type="entry name" value="Mediator_Med6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016612">
        <property type="entry name" value="Mediator_Med6_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038566">
        <property type="entry name" value="Mediator_Med6_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13104">
        <property type="entry name" value="MED-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04934">
        <property type="entry name" value="Med6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF013286">
        <property type="entry name" value="MED6_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0010">Activator</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 11" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="210"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="214"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR013286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="268" mass="29967" checksum="71F91211C3D45F0A" modified="2011-11-16" version="1">MEALDEIQWKSPEFIQERGLHSNNVLEYFALSPFYDRTSNNQVLMMQFQYQQIQIPMNMTFHQYFQSRLTEMTGIEFVIAFTREPDFWIVRKQRRLDPTSTLILQDYYIIGANVYQAPRIYDVLSSRLLSSVLSLRNSIDILNNMTSYHISDGGHSYNNSIHGVTSTGSSSQLPGNAQSKSLTMTVSNTNVMNTMTPITMNTPGLALAGPRTGLPPSTAASSNALATSNSQTEITSGSEDNKLVYLDDIPLYGKGSTVEMLGLKTNFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARH6</accession>
    <name>G3ARH6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glutamate decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_61865</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376075.1">
        <property type="nucleotide sequence ID" value="XM_007376013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARH6"/>
    <dbReference type="STRING" id="619300.G3ARH6"/>
    <dbReference type="GeneID" id="18874086"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61865"/>
    <dbReference type="eggNOG" id="KOG0629">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011856_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARH6"/>
    <dbReference type="OMA" id="RHATYHA"/>
    <dbReference type="OrthoDB" id="2161780at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45677:SF8">
        <property type="entry name" value="CYSTEINE SULFINIC ACID DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45677">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 6 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="499" mass="55587" checksum="EBDA95669D70CF40" modified="2011-11-16" version="1">MTQASISSDLESRRVEELDRLLTLLKPRILQYVESADPQSANYNPTSLGTYHEPEFLKQKILNGDDLAEKCSEDELFNVIDKVLEYSVNTWNPGFLDKLYASNNPIGVVSDIILSMLNTNSHVYTVSPVLSVIENYIGRKYARLFYTKDQETCGGLTFAGGSWSNVTSLQIARSMRFPDTKVKGNQGYKFAIYTSKHSHYSVEKAAILLGLGSENVFKIDVNAVGTMDVAKLRETIATTKSQGYTPLYINATAGTTVFGSFDPFTEIAAVAKEFGVHFHIDGSWGGNVIFSSKYRGRLAGCELADSITVNPHKMLGIPNTCSFLLLPHVAHFQTAMSLQAPYLFHGRESGEENYDLADGTMGCGRRSDAFKFYMGWLYYGTAGFEARVNHAFTIMEYFVARISADSRFELVGSSKENLPQCLQVCFYYHPSCGCDNTKVTRFISRELHKMGKYLVDFSPNADGSDKGEFFRVVFNSPILTNDVIDDLIDSIVKVGERCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARH8</accession>
    <name>G3ARH8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA damage-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_140061</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376077.1">
        <property type="nucleotide sequence ID" value="XM_007376015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARH8"/>
    <dbReference type="FunCoup" id="G3ARH8">
        <property type="interactions" value="1335"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARH8"/>
    <dbReference type="GeneID" id="18870266"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140061"/>
    <dbReference type="eggNOG" id="KOG1898">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003246_0_1_1"/>
    <dbReference type="InParanoid" id="G3ARH8"/>
    <dbReference type="OMA" id="PRATGHW"/>
    <dbReference type="OrthoDB" id="436637at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="5">
        <location>
            <begin position="19"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="6">
        <location>
            <begin position="406"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="4">
        <location>
            <begin position="769"/>
            <end position="1074"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1107" mass="123406" checksum="CB5E7998679C2449" modified="2011-11-16" version="1">MSDSLHLYNLTLKDSSHYTHTLLGQFHPKDPATQFLVLISPTHIDLVQPNPDTGKLQLIHHQKVIGVINQASVIRVEELDHVVVTSDSGNLSVLKFDGTKFVPAMNIGMTKNGWGRTYPGEFLAVDPSNRCVFTSAVEKNKLIYKVDSELSSPLEAHFNGVCLGVCALDTVYENPCFAVLEMEDQVALNYYHLDLGLNHIIKSKSDPIPQDVNYVIGLPGHIGGVLVSGTNWMCIDRKNERKFFPLPRRKGQDTMITSHVLHRLKKNEFFILIQSSLGDLYKVVVEYDNDREVVKDVIVSYFDTIPPCISLNIFKSGFLFANVLNNDKLLYQFEKLGDESTTVIKSSQYPDHTEEVNITLKALDNLALIDIHESLSPILDAKQTPSNMLVLSSHYLKQVTRGIPTTEIVSTPLPFTPTSVYTTKLTQASVHDSYLVVTSSLASQTLVLSIGEVVEEVPDSKLVTDQHTLSIQQMGKSSLVQVYTNGIRQIGHKVTDWFSPAGITVTHASTNANQVIIAMSNCEVVYFEIDVDDQLIEYQQRLELTSSITSLAISDTRSDVAVIGCADETIQVVSLAESDCLDVKSLQALSANASSLVMIENTVHIGMENGVYARTKLDIKQLKDTRVQYLGSRPVSMSRISVGDTAGVLAISSSAWIGYTHDTWRIMPLLDIDISSAASFNSEDIEGIVGIHGNELVIFSLGTEDGFDPSHEWTIKKSRLRYTPRRMVVDGEKVYVIESEYGIKSPYHTQDTDQDYYDAFGYERGGPGASCIQVVINEEVTQSIELEHTAISMTKVTFKNQPCLVVGVTTKQTYLPTSHDECFLYTYKIGDTLELLHRTKLTIRPTVLIAFNNRVLVASGNHILLYELGQSQLLRKSASVLAYLAQINNVIHIESSRIVITDAHSSSISYAKFDTGLNQFVGFADDVIKRQVTAIVSVDYDTVISGDKFGNIRVSRVDEEVSKQTDEHWSFIKQSDGLFNSTGSKLKSLASIYVHDTPIKMMKSKDCIVWTGLMGTIGSFIPLLTTSEVDWLRKLELAMRNQVVNLLEKDHLKSRSYYDPVKCVIDGDLLEMYYSLSMDVKLSIARQVNRSPSEIEKKLENLRNRVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARS3</accession>
    <name>G3ARS3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sulfatase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_154340</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfatase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376117.1">
        <property type="nucleotide sequence ID" value="XM_007376055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARS3"/>
    <dbReference type="STRING" id="619300.G3ARS3"/>
    <dbReference type="GeneID" id="18870978"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_154340"/>
    <dbReference type="eggNOG" id="KOG3867">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006332_11_1_1"/>
    <dbReference type="InParanoid" id="G3ARS3"/>
    <dbReference type="OMA" id="YSDQWAQ"/>
    <dbReference type="OrthoDB" id="103349at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004065">
        <property type="term" value="F:arylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019637">
        <property type="term" value="P:organophosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16025">
        <property type="entry name" value="PAS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050738">
        <property type="entry name" value="Sulfatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693:SF33">
        <property type="entry name" value="ARYLSULFATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693">
        <property type="entry name" value="ARYLSULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00149">
        <property type="entry name" value="SULFATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="5">
        <location>
            <begin position="10"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW31339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="566" mass="64076" checksum="C6E36BF13D35CBFF" modified="2011-11-16" version="1">MTIPSTPKKPNFLIIVADDLGFTDTSPFGGEIHTPNLHKLALNRGVRLTDFHTASACSPTRSMLLSGTDNHIAGLGQMAEFASRHPEKFQGKPGYEGYLNDRVVALPEILRDAGYHTFISGKWHLGLKKEYWPINRGFTKSWTLLPGAGNHYKRVLRDDQGNQLPFLPSLYVEDDKELDPEKDLPEDFYSTTTFTDKAIEFIDSTPEDKPFFGFLTYTAPHWPYQAPQDRIEKYKGVYDNGPEELRRARLARAAKLGIIDSSVTPHPIKTIRKRWSELSDKEREVESKIVATYAAMVEILDENIGRVIDHLEKKQELDNTFVLFMSDNGAEGMLMEALPLTNQRINNFIKNHYDNSVENIGNKDSFVFYGDQWAQAATSPHAMYKMWSTEGAIVCPLIVHYPPLLEQNHGKILNKFTTVMDILPTVLELAGVQHPGKEYKGREVAIPRGKSWVSYLGDKSGQVHSDDTVTGWELFGQQAIRKGDYKAIYIPKPFGPEKWQLFNIIADPGEIHDLAETEPKVLEELVGHWAVYAAETGLIELGPDLFEKEKIEGEDNEVIYRIILDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARW0</accession>
    <name>G3ARW0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent RNA helicase DBP10</fullName>
            <ecNumber evidence="4">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP-dependent RNA helicase dbp10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">SPAPADRAFT_140104</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits and is required for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family. DDX54/DBP10 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="4"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376155.1">
        <property type="nucleotide sequence ID" value="XM_007376093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARW0"/>
    <dbReference type="FunCoup" id="G3ARW0">
        <property type="interactions" value="1112"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARW0"/>
    <dbReference type="GeneID" id="18870268"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140104"/>
    <dbReference type="eggNOG" id="KOG0337">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_5_1_1"/>
    <dbReference type="InParanoid" id="G3ARW0"/>
    <dbReference type="OMA" id="EDQFGMM"/>
    <dbReference type="OrthoDB" id="10261375at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17959">
        <property type="entry name" value="DEADc_DDX54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012541">
        <property type="entry name" value="DBP10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033517">
        <property type="entry name" value="DDX54/DBP10_DEAD-box_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF8">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08147">
        <property type="entry name" value="DBP10CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01123">
        <property type="entry name" value="DBP10CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 20" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="19">
        <location>
            <begin position="102"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="133"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="371"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="826"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="15">
        <location>
            <begin position="102"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="17"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="854"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="887"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="909"/>
            <end position="918"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EGW31377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="918" mass="105018" checksum="8761ADC966463773" modified="2011-11-16" version="1">MSDDEDFDIAGSLGLNQEEEEDYSSSDNESEQEIQDEIISSDEEEQADEPPKKKTKPTKKQESFPSLELSDNEQDREDADDDSQLNSYFIANNPATKKAKAGSFASFGLSKFLLTNIAKKGFRQPTPIQRKTIPLVMENRDVVGMARTGSGKTAAFVLPLIEKLKVRSASGVRAIILSPSRELALQTYKQVKEFGHGTNLQTTVLIGGDTLEEDFSKMVSKPDIIVCTPGRFLHLKVEMDYDLSTIQYIVFDEADRLFEMGFAIQLNELLASLPPNRQSLLFSATLPRSLVDFAKAGLTNPVLVRLDAETKISDQLQMAYFTTKKNEREANLLYILQEVIKLPLGSPEQIKKLKDMDKRQVESDEENEEDGKKKKYKFKKERMPAANQLPSEFSTIVFVPTKHHVEYVTRMLRDAGYLVSYIYGTLDQHARKNQLYQFRIGLTHIMVVTDVAARGIDIPVLANVINFTLPGSSKIFIHRVGRTARAGNKGWAYSIVNEKELPYLLDLELFLGKKILLTSMHETKCEVLKRKQGDAYVPPKVNYTERLVLGSIPRLDLEGCQELYENLLRNDYELKVLRDVAAKGEKLYHRTRQAASPESLKRSKEIMETGSWDEQHVLFGPNLEKQKQDFLAKLQNRNVKETVFEFNKKGNDKDENSLVEFMHRRRRQIAPIQRRAKERRELLQKERNAGLTHGIEDEILHEEGETGYNADEQELEQVFEDADKISSKKKTYRDPNFFLSHYAPASVIQDQQLSISSSFANDAQAATFDLDNDDKIQKNKQTMAWDKKKGKYVNSKSTDVKYIIGESGQKIPATFRSGKFDEWKKKRGANLTTESTGESDNKRFKHKKVQTPKLPDKFRDDYHKQKKKVEKAVEGGMKVRGYNKPGHQQEIKSTEQIRKARMVKEQRRAKNARPSRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS33</accession>
    <name>G3AS33_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_62488</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376659.1">
        <property type="nucleotide sequence ID" value="XM_007376597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS33"/>
    <dbReference type="GeneID" id="18874345"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62488"/>
    <dbReference type="eggNOG" id="ENOG502T6HB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_559177_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS33"/>
    <dbReference type="OrthoDB" id="4159781at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051430">
        <property type="entry name" value="Fungal_TF_Env_Response"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31944">
        <property type="entry name" value="HEME-RESPONSIVE ZINC FINGER TRANSCRIPTION FACTOR HAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31944:SF131">
        <property type="entry name" value="HEME-RESPONSIVE ZINC FINGER TRANSCRIPTION FACTOR HAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="8">
        <location>
            <begin position="71"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW31881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="488" mass="56745" checksum="FCC11F85B2483102" modified="2011-11-16" version="1">MAHLTLFNFTTRGSSNLVDYPISLDVIRAASTIMKEFDLSRRQPLIVLQAGLLFRVYLGWAPENFTSGSRTQISMGTIVQLAYSLGLNRDPTCFTITTPRQANLRRKIWHVLVRLDVLDSMIWGTTLSTDPTTYDTQLPTYEEVSCNLEDKSLERDIITSFPKYDALLSVCYKLTGIHLRVKGGHKLSEVTSLISELEVLCQRLLGTVKQGLKSDNFLDLLKLRILLFVKLYLVYNHYNLYLYYEAQQNPLQMYYFRKVLEIVMVDMKEFPYQAMNTEGNGFSLMFASILQVYLHVVFLIIMCIRSRFISTLYEIKRNPARFKHDSLLEYTKTIEDSDAALKTFSYCNLRMTRILGDRYKYSWILSKVHMNILRLYDDNLEIFNYNESKAVKEAVIDMSLEDIRHITTLTEGCIRANKFGPTPFGNESEEELIQEIQRDNLWHQIKKIFTEETVTSAWIDKSKYNNNSTITFDFDLFVNDINFAQSIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASW9</accession>
    <name>G3ASW9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Copper resistance determinant 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_56729</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376785.1">
        <property type="nucleotide sequence ID" value="XM_007376723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASW9"/>
    <dbReference type="GeneID" id="18872063"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56729"/>
    <dbReference type="eggNOG" id="ENOG502SCEH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133919_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASW9"/>
    <dbReference type="OMA" id="CTCEKAT"/>
    <dbReference type="OrthoDB" id="273010at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057193">
        <property type="entry name" value="DUF7871"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40620">
        <property type="entry name" value="RESISTANCE PROTEIN CRD2, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G04318)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40620:SF1">
        <property type="entry name" value="RESISTANCE PROTEIN CRD2, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G04318)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25277">
        <property type="entry name" value="DUF7871"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7871" evidence="1">
        <location>
            <begin position="16"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="76" mass="7874" checksum="EF572A42C7D1C0A2" modified="2011-11-16" version="1">MTCSSAECVCAKKSTCSCGARPAQQCNCDKATVENVAPAAGDACVCGKRNKDACTCGSHSHCDGHREGETDFTNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASY2</accession>
    <name>G3ASY2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_62615</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376797.1">
        <property type="nucleotide sequence ID" value="XM_007376735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASY2"/>
    <dbReference type="FunCoup" id="G3ASY2">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASY2"/>
    <dbReference type="GeneID" id="18874407"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62615"/>
    <dbReference type="eggNOG" id="KOG3480">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_162151_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASY2"/>
    <dbReference type="OMA" id="EKCIPHE"/>
    <dbReference type="OrthoDB" id="274922at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042719">
        <property type="term" value="C:mitochondrial intermembrane space chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11038">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11038:SF18">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0143">Chaperone</keyword>
    <keyword evidence="10 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="12">
        <location>
            <begin position="23"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW30764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="101" mass="11536" checksum="95E0772C97E02BDE" modified="2011-11-16" version="1">MSLFLGNSLQYSQATVDPEKLKLAEVQFTATAHTFNKLLQSCANKCLVHEYGEGELSKGEQECIDRCAAKYVRANFLIGKHFQEQRLDPMSSLVNYEIYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT06</accession>
    <name>G3AT06_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_63080</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377199.1">
        <property type="nucleotide sequence ID" value="XM_007377137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT06"/>
    <dbReference type="FunCoup" id="G3AT06">
        <property type="interactions" value="86"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AT06"/>
    <dbReference type="GeneID" id="18874587"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63080"/>
    <dbReference type="eggNOG" id="KOG0417">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_13_0_1"/>
    <dbReference type="InParanoid" id="G3AT06"/>
    <dbReference type="OMA" id="WRAVMKG"/>
    <dbReference type="OrthoDB" id="9973183at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016562">
        <property type="term" value="P:protein import into peroxisome matrix, receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23812">
        <property type="entry name" value="UBCc_ScPEX4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="4">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW31166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="190" mass="21648" checksum="7C8424BB52581EA1" modified="2011-11-16" version="1">MAERRLFQEYNRYKKSPPSKSNPQIISLNPISEDNIFNWQAIISKPTKKDNPFYYGGQWKLSIAVSRQYPLEPPVIKFITPIVHPNINLKTGEICLDILKKDAWSPAWNLEHLLVAILMLLDSPEPDSPLNIDAANLYRHDPVGFQSMVQFNMWKVGSFVDVKKSTPTTITKSNQVIRDVSGSKIRAIIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATH6</accession>
    <name>G3ATH6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_142162</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376972.1">
        <property type="nucleotide sequence ID" value="XM_007376910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATH6"/>
    <dbReference type="GeneID" id="18870426"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142162"/>
    <dbReference type="HOGENOM" id="CLU_2090631_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATH6"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="117" mass="13105" checksum="729AA0E5A413DAE0" modified="2011-11-16" version="1" fragment="single">VNRSNCSSRKSTKPTNFLFSASNCRTLSSRVSVYPLGNALRRSLSDTIHSSPTARQVRHGFWPSHLVFFARHRSHAWATLSTFDPGAFIVFALWSFEPVIVSSLSDMVKACKLTEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU13</accession>
    <name>G3AU13_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_68493</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377359.1">
        <property type="nucleotide sequence ID" value="XM_007377297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU13"/>
    <dbReference type="FunCoup" id="G3AU13">
        <property type="interactions" value="264"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU13"/>
    <dbReference type="GeneID" id="18875222"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68493"/>
    <dbReference type="eggNOG" id="KOG0583">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009275_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU13"/>
    <dbReference type="OMA" id="DHAFRDY"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000949">
        <property type="entry name" value="Serine/threonine-protein kinase PTK1/STK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="222"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="131"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="567"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="626"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="669"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="719"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="799"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="131"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="567"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="626"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="731"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="745"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="765"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="832"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW30388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="895" mass="99407" checksum="F0D1B4ED1DD44184" modified="2011-11-16" version="1">MPLTRSSSQTNTHHHHRHIKNIFKREKSPDDTPSSNSSSTVGLSKLFHHTKEAENDHPELKPPSRVPSAFAIRRHNTNPGRLRSNSEKIPTHSESSSPVQQQHAGQGHPPFHQPKKLTKAETFAHLQQLNTKNAHQQQLRSSRLPSNHNNIATPTSAEKIVYNPYGLNKNPSQEGPKNTSFYLSGVADGERVVANPVADPNDYLPEDLKQASVNLLEDYEIDVGTKKLGAGGSSDVRIINSVHNKKIVFALKKFTLLSKETDEEFYKRVSKEYVIHKKAAISRHVVDTTAIVRIHSQTNLTRGWGMVLEFCGGGDLFSLIVKPGWKRAPLNEKFCLFKQIAYGIKFLHDNDIVHRDIKPENVLLDANGLAKLCDFGVSEYGHEIPGDLTSPAKLSCSYVGSPPYSPPEVMLLKEKSHTEVKNFAYDPFKMDHWGLGMMLFCLVYCGVPFQHASPNDHAFRDYKFSHKRFCSDHQAFRNNVGYTKGPGSEFKLAAKFESTGASRVAWKLCDPSPESRYNLDMLFNDPWFQSLEMCIYEHPDQEVNPFVLPGTGENMPGSGQSNFPYSSAGSVAGSAAPSRRATFNRNPRLEEESVIATGELPANSSLRSMLDLGDVTKKINEIKVTQRGSNAATPMSPQTPSHASDFTPGSTPDINGGVRRVKSMLDVGKSENTGSTVTPMPVTPPSPIEENLESTGDNLPAVTETEDEHVPLVQEMTKIPENVQAEEEAEEAIKEEKIKERPSQESESSGIGEQESIFPLPLPRNDTKIKRDHSSSSLQYDTSFDHDISLQYDSTLQSDLQTSLRKDTSMTENISSQEKEMKQLQVEQSSSQEKELSDSEKADARSLAELKPKLLKSIPDLQLDKDGTCDLGYKIKKHNHTGVSGAEISGAVRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUJ1</accession>
    <name>G3AUJ1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NTF2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_63389</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377518.1">
        <property type="nucleotide sequence ID" value="XM_007377456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUJ1"/>
    <dbReference type="STRING" id="619300.G3AUJ1"/>
    <dbReference type="GeneID" id="18874725"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63389"/>
    <dbReference type="eggNOG" id="KOG0116">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036630_1_0_1"/>
    <dbReference type="InParanoid" id="G3AUJ1"/>
    <dbReference type="OMA" id="KTYYQRM"/>
    <dbReference type="OrthoDB" id="339151at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990861">
        <property type="term" value="C:Ubp3-Bre5 deubiquitination complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034517">
        <property type="term" value="P:ribophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00780">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039539">
        <property type="entry name" value="Ras_GTPase_bind_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10693:SF20">
        <property type="entry name" value="AT27578P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10693">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="NTF2" evidence="3">
        <location>
            <begin position="62"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="219"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="221"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="299"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="321"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="358"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="398" mass="42545" checksum="8E8764C1D058ED5A" modified="2011-11-16" version="1" fragment="single">MTTSPSPAPPTTAGSAPVSPEIANEATTAAAATTANAAATATAATTTNSAPASLTEERTTTIGWFFIQSYYDFFVNKLDTIHKIYHANAVLSHDAYPTTSQDKVPATTHTARGIDAIKSRFAVEEEQATTNRIVITSATFQTSLDKNIIIVAFGEWAKSDSDGFRQFTQTFVLTPGKKENTFDVANDILKFIDVNGFTELNSEVKNKPAGEVEETAVVAVAEEESKEEQQPEEEEQEEEEEEQGEQEVNTTEETAVDSEPVSEVKEEETNGEVKVQVEPEEEEEQQQQQSEEPKSEAETSVSTPVATAATPSKPLSWADLASQSSAKAASAKAASPAASATAKPAAPAATGVTLKKQPPTSASPTGSSPTSNASGKFKKEDWFPIYIRGIKQLEEKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUM4</accession>
    <name>G3AUM4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_63417</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377551.1">
        <property type="nucleotide sequence ID" value="XM_007377489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUM4"/>
    <dbReference type="FunCoup" id="G3AUM4">
        <property type="interactions" value="925"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUM4"/>
    <dbReference type="GeneID" id="18874740"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63417"/>
    <dbReference type="eggNOG" id="KOG0759">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_14_1_1"/>
    <dbReference type="InParanoid" id="G3AUM4"/>
    <dbReference type="OMA" id="IMPALNW"/>
    <dbReference type="OrthoDB" id="448427at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005310">
        <property type="term" value="F:dicarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000107">
        <property type="entry name" value="Mitochondrial dicarboxylate carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 13" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="4"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="98"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="199"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW30580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="287" mass="31408" checksum="F34393178C2447A1" modified="2011-11-16" version="1">MSSSKVQYPFWYGGASSMAACMVTHPLDLAKVRLQTASERGQGLVSMIYQIITKEGPTKIYSGLTASLLRQATYSTARFGIYEWLKDQYIESTNNTKPGTMILLPMSMLAGGLGGLIGNPADVVNIRMQNDNTLPVEQRRNYRNAFDGLYKICRDESPASLFRGLMPNLVRGVLMTASQVATYDIAKTFLVDQLNLDPTKKTTHFGASLIAGLVATTVCSPADVVKTRIMNSKGSGGSAVTILTTAVKNEGVGFMFRGWLPSFIRLGPHTIVTFLVLEQLRKYRVGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUQ1</accession>
    <name>G3AUQ1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 45</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_143323</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377578.1">
        <property type="nucleotide sequence ID" value="XM_007377516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUQ1"/>
    <dbReference type="FunCoup" id="G3AUQ1">
        <property type="interactions" value="862"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUQ1"/>
    <dbReference type="GeneID" id="18870534"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143323"/>
    <dbReference type="eggNOG" id="KOG1299">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013933_3_1_1"/>
    <dbReference type="InParanoid" id="G3AUQ1"/>
    <dbReference type="OMA" id="VQVTRHC"/>
    <dbReference type="OrthoDB" id="10266265at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048210">
        <property type="term" value="P:Golgi vesicle fusion to target membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035543">
        <property type="term" value="P:positive regulation of SNARE complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="611" mass="69643" checksum="1631D6B5470E2E64" modified="2011-11-16" version="1">MTLNLSKVKEFYFNKLFSPKDKSTATASSPKVLLVDKYTTPIISICFTQSQLLSNDVILVELIEDQEQLSTMRHLNCIVYIHPCAQSINHLVRELHSPHFQNYQLFLNNTVSKNQLESIAEADEFEVIEKVVEIFQDYLIVNENLFSIDLSDSVKQVNPAIEESNKLVSLLLSLKKTPVIKYESNSLDLKRLSSEILYNINTNSNNNLFDDLNKNSDVAPILLLLDRKNDPITPLIAPWTYQSMIHEFIHINKNIVDVSGETVILDSQFDKFFKEAMYLNYGDLTEKFQKYVEEYKTQTKSTSLDNLQNTNLSDLKKMLTKFPEFKKLSSNILKHLNLLSEIDKQISAQNLWEIGELQQVIICGLENHQAVKTRLLEVLDNPHSTTTINKIKLVLLYSIRFHNPSELSDFITKLNDPSLTNPTPTYSQNELLKRFKTLFNSNITSAATSSSDNQLTNIFNKKINFKTLFNRDNSAQHNDNVYLQYTPRLNELLSGLINPVSDSIPASIQGLSTLVPDKVKQQYGNSVATSSAQVQDIIIYIKGGVTYEESRLIYELCESSTTSSKINLIIGGDKVLNSDIWLNNLYDMVNEESPAVASERIDRQSQLREIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUS5</accession>
    <name>G3AUS5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_143721</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377602.1">
        <property type="nucleotide sequence ID" value="XM_007377540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUS5"/>
    <dbReference type="STRING" id="619300.G3AUS5"/>
    <dbReference type="GeneID" id="18870566"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143721"/>
    <dbReference type="eggNOG" id="KOG0061">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_57_7_1"/>
    <dbReference type="InParanoid" id="G3AUS5"/>
    <dbReference type="OMA" id="FWYYTFY"/>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052215">
        <property type="entry name" value="Plant_ABCG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48042">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48042:SF11">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="407"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="448"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="486"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="569"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="8"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="593" mass="66847" checksum="DFF7EDF8C0B57325" modified="2011-11-16" version="1">MSSKAQVLSWSNINLQLTNGTKQVLIDNVSGSVSAGQMLAIMGPSGSGKSTLLNILAHRSKPGSSIQSGEIKINNEHANLAKIKQLSSYVEQEDHLIGSLTVLETVDYSARFAGVDKNFRKQLVDKTISLLGLENQRDVKIGSPIQKGISGGQKRRVSIASQMITTPSILFLDEPTSGLDSVASREVISTIKRIASEENMIVICSIHQPSTYTFELFDDVLFLSRGKTVYHGKVEKVVKYFDSVGYTIPPYINPSEYVLDLINTDFTVDQDADEKMEVLSDLVNKWKSTHVAAAKPESEINEIELDKFDAKTEVKNTFILVSRVLIKARRDILTYYVRMVMYLCLAILMGTVWLRLGKGQQNIQPFINAIFFSGAFMSFMSVAYIPSYIEDLLSYKKERLNGLYGPFAFTISNFLVGLPFIFLIATLFSVITFFMVNFNSTATGFFYYLMWLFLDLVAAESMTIFIASIFPNFVIALALTAFANGLWMAVGGFLVPSNILNVFWYYTFYWVDYQRYVFQGMMFNEFDNRNFDCDNNCFCMYDSPLASQCMIKGSAVLDHLGYGKYDKGLWIGVLIAIIFFYRVASYLVLRFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUT5</accession>
    <name>G3AUT5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63644</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377792.1">
        <property type="nucleotide sequence ID" value="XM_007377730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUT5"/>
    <dbReference type="FunCoup" id="G3AUT5">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUT5"/>
    <dbReference type="GeneID" id="18874849"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63644"/>
    <dbReference type="eggNOG" id="ENOG502T76D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069662_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUT5"/>
    <dbReference type="OMA" id="HWSNLPF"/>
    <dbReference type="OrthoDB" id="4069757at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="250" mass="28493" checksum="C6C25961B133F4C4" modified="2011-11-16" version="1">MSQHSSRSPSIYNEFAPKTCIIISNLHKNDFIIVDSNTPQLLSKKLSLVDQIKLSVLNINETPDFIEQISYWSSLPFLNRIIIIFRDESAASIAYKHLTQGATSLQGLYPYVKVALQENLLQRSKSTDNLDNDNLAVAKSLEKFKNFHNDPDNIYKNYSEPEPQQFNVLLDLPKLGIDLSDYNSEEQMEELKQDELQSRSLSPERPPALRRSSTKTLYKPSLHLNTESANKTHRASDFATSPTITLDETF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUV8</accession>
    <name>G3AUV8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Peroxiredoxin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_63665</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxiredoxin family. Prx5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377815.1">
        <property type="nucleotide sequence ID" value="XM_007377753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUV8"/>
    <dbReference type="STRING" id="619300.G3AUV8"/>
    <dbReference type="GeneID" id="18874857"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63665"/>
    <dbReference type="eggNOG" id="KOG0541">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072440_1_1_1"/>
    <dbReference type="InParanoid" id="G3AUV8"/>
    <dbReference type="OMA" id="ERHIPEY"/>
    <dbReference type="OrthoDB" id="195498at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037944">
        <property type="entry name" value="PRX5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430">
        <property type="entry name" value="PEROXIREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430:SF16">
        <property type="entry name" value="PEROXIREDOXIN-5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Redoxin" evidence="7">
        <location>
            <begin position="38"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="6">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR637944-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="186" mass="20240" checksum="FD11D094A1249450" modified="2011-11-16" version="1">MSLTEGQQFPDNVEFTYIPIQLDDLALINPLKCDTSAQLKVDELLTKLSTTDTPNLLIVSVPGAFTPTCTEVHIPQYLENLPKLTEAKVGALLVLAFNDAFVVNAWGKVLIKEYVKQSDKALPQVIFASDGGFSGAHDLAADRGGVLRNKRYATVVNAKDRTIKYLGVEVERGVHNSGLDAVLSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUZ2</accession>
    <name>G3AUZ2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase</fullName>
            <ecNumber evidence="4 14">4.2.1.134</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_63465</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a very-long-chain (3R)-3-hydroxyacyl-CoA = a very-long-chain (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:45812"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:83728"/>
            <dbReference type="ChEBI" id="CHEBI:85440"/>
            <dbReference type="EC" id="4.2.1.134"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endoplasmic reticulum membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the very long-chain fatty acids dehydratase HACD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.134" evidence="4 14"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377615.1">
        <property type="nucleotide sequence ID" value="XM_007377553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUZ2"/>
    <dbReference type="FunCoup" id="G3AUZ2">
        <property type="interactions" value="545"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUZ2"/>
    <dbReference type="GeneID" id="18874766"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63465"/>
    <dbReference type="eggNOG" id="KOG3187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034302_6_1_1"/>
    <dbReference type="InParanoid" id="G3AUZ2"/>
    <dbReference type="OMA" id="WSYILWQ"/>
    <dbReference type="OrthoDB" id="46988at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102158">
        <property type="term" value="F:very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030497">
        <property type="term" value="P:fatty acid elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007482">
        <property type="entry name" value="Tyr_Pase-like_PTPLA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035:SF3">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04387">
        <property type="entry name" value="PTPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 14" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7 14" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW29849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="226" mass="25505" checksum="BADC06B51A9A5B1C" modified="2011-11-16" version="1">MSAPVKSAPAKSNQSAHPQRYLIAYNSISASLWSLVLFNTVFLALTVGQPYVFIKTNFSTALIQTLAVVEIFNSALGFVKSPLFTTVTQVFSRLLIVWGIHQLLPQSPANFHWCYITLCLSWSITEIIRYSYYASNLRAGASVVPSQYLTWLRYSTFYVLYPTGVFSEVYSVILSLGEAENVVGAWYAWALKAILVVYIPGFYMLYTYMIKQRKKVLGANKVKKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV09</accession>
    <name>G3AV09_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_144352</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377849.1">
        <property type="nucleotide sequence ID" value="XM_007377787.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AV09">
        <property type="interactions" value="231"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV09"/>
    <dbReference type="GeneID" id="18870619"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144352"/>
    <dbReference type="eggNOG" id="KOG0605">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000709_2_1_1"/>
    <dbReference type="InParanoid" id="G3AV09"/>
    <dbReference type="OMA" id="HNRRFVG"/>
    <dbReference type="OrthoDB" id="162894at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036180">
        <property type="term" value="P:filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900445">
        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901992">
        <property type="term" value="P:positive regulation of mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000340">
        <property type="entry name" value="Serine threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001008">
        <property type="entry name" value="Serine/threonine-protein kinase cek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE GREATWALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="832"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="1302"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="13">
        <location>
            <begin position="1634"/>
            <end position="1748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="26"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="403"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="667"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="719"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1335"/>
            <end position="1399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1446"/>
            <end position="1622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="404"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="676"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="746"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1340"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1367"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1388"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1467"/>
            <end position="1477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1578"/>
            <end position="1587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1595"/>
            <end position="1607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW30083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1756" mass="192040" checksum="31688862CF79890A" modified="2011-11-16" version="1">MSNQSTPRIQTPIQDEDVLFYKPIFESTPLSHQPSERSLSPREIPDQSKSRNGPPPQSNSLVSIAEISPATSNRSIEEQIDLRLASSNNPTIVMELDLDGNIRYISKSWEYIVGTNIRKIINCPISKIIIGNNDDDFKVFNNAIDQMIKDDGSYKVKFITATNNAPRMEPSESMKQEQEMYNDLKRLLSRPGDLDNDNQQEINMTPPNSAVESEAESEIELPVEDYIPGYIRTGSRAIKIQDGAEQSSLGSQEDTLSQLSSTLSNDGGIIELEAQGILIHDAKTNLPSHSMWTIRPFQHIDLDLTLPNALIDLLGFGSEIFEGYLLNLKELAIIDEESVPQPKTVLCRICEAYFPAWFIEKHSDLCILEHRAKENLQQCHDAISEQRDLVLKIIESLAQQHARSQSGSQQSSSESISPTSSISTLSSSSSLSSSETPSPVIYDYKGIPLPIVGDIANASPSLANQVLTKNFQARSGSLIQSRKFPFGILQKVVDICDDALLVNPPSNNEEHVLEFSPSSEKALNNVIARPILETSDFAIKQIIEDTQALIDNKMDTLSRLISILQYMDKINKEVDDLVLKTVRETVTKIKEQTISEIDQEPPRVSTPDIIHLDQSSTMPLRPLPVPVSNHIIQAPRPSKSRASPASELFSSPFESVVTPRDILLSREHSKRHSESNHSVSSASPMSISHPSSKLGHHHSDHSSRNNSRDLLESIQNLDLSKKSSENNSSFSSPRRHLSPAPYVEKSNLTSLQKNTASSRYTPVSSPMITHDEGGDHKKITKLSLTTTDAYGSTMPITPQSVSMQKMTAPLSPLLVSSTASSKPISGGGVRDYEVIKPISKGAFGSVFLAKRKLTGDYVAIKCLRKRDMIAKNQVLNVKSERAVMMRQTDSPYVAQLYCSFQSRDYLYLVMEFLIGGDCAMLIKNLEVVGLEWSARYIAEIIVGVDDLHKRGIIHRDLKPDNILIDKDGHLKLTDFGLSRLGVVGRQNTHRKSSTNDQGIELFRSTLSVEPGLHEFHHKRTSSGTPFTLSPTIEHAKLNTINNLNTINNIQQQHALQHQLSSPTLSFMEAFSGTSPSTPGSSTLATPIGPIFPLPAPPPPGASHSGSLSNIFPHRSSLLKGRSNSSGLDSPVLKPIIPRTSSESSFAILDEDYSHGGTGSGSSSSATGFGPITTMGNQTGVNYALYDPQNEHEFKKFVGTPDYLAPETIEGIGQSEASDWWSIGCILFEFLYGYPPFHADTPDQVFRNILECKIDWPGLPPEEDLNFCSPEAKDLIIKLLTLDPHERLGYHGAEEIKSHPFFKDIHWDTLFEEPAPFVPIVDDPASTDYFDPRGVGVVQFPKDDSDTDDNKSDVEREGGTFGDVVHRSSINTNNSGESPGGSFSVPGTGHSGGSSGSAGIGIQFKKERRSSRLADPGEFGSFHFRNLSVLEKQNKDVINRLKTEHLEHRNSFSSSSSDSPKLRSRGFSFGGIGSGGTVPGSVSGSIGSGSGVMSGSPFKRPVSPSTPHSLSLGSPAGAPGGGSVRSASPNRIFDSPVTGGNVGVGRQERIASAVSTYSSGDEGMAGSANGSGSEEKLRSSSMAISKHSSLLRDLNSPLTSDNEESSNALFRVQQRRKRENSRMDNDSISIANELDVLYCEPISVVRHSVVKLLERAGCIVVSITDGEELVKRATSRVKFDLIFTALKLPHVEAIDGVKLIKFTTGKNSQSPIVAVTGFPEEAKESGVFDYIITKPVDFDQVQRCINKFTNEVAILDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV44</accession>
    <name>G3AV44_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">DNA replication complex GINS protein PSF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_63735</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the GINS complex which is a heterotetramer of SLD5, PSF1, PSF2 and PSF3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GINS1/PSF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377884.1">
        <property type="nucleotide sequence ID" value="XM_007377822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV44"/>
    <dbReference type="FunCoup" id="G3AV44">
        <property type="interactions" value="435"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV44"/>
    <dbReference type="GeneID" id="18874888"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63735"/>
    <dbReference type="eggNOG" id="KOG3303">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079191_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV44"/>
    <dbReference type="OMA" id="MFCEKAT"/>
    <dbReference type="OrthoDB" id="10252587at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902983">
        <property type="term" value="P:DNA strand elongation involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11710">
        <property type="entry name" value="GINS_A_psf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21696">
        <property type="entry name" value="GINS_B_Psf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005339">
        <property type="entry name" value="GINS_Psf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056783">
        <property type="entry name" value="PSF1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12914:SF2">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12914">
        <property type="entry name" value="PARTNER OF SLD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24997">
        <property type="entry name" value="PSF1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GINS subunit" evidence="9">
        <location>
            <begin position="51"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="DNA replication complex GINS protein PSF1 C-terminal" evidence="10">
        <location>
            <begin position="175"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="222" mass="25670" checksum="837AE0093F5CD62C" modified="2011-11-16" version="1">MYGDIANKLILEAKRSVNLSEVPLYQTELVKDIIKEINDLNRDADYLTEEQQLLQQESQDGTEEESKINQCQLFVTILSMRRNKRCLLAYEKLRADKLVEFSWLNVDPIVEGNDKQPTSVPVDPIYTNYTTQVALDNLNHAEQEFLKNYQELLLSYKSSFSDVDLSGDLNPPTNIFIDVRVLKDGGEVQTEYGSFNLIKDSQFYVRKSDVERLIQQGYLEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVA6</accession>
    <name>G3AVA6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ubiquitin-conjugating enzyme E2 2</fullName>
            <ecNumber evidence="3">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">E2 ubiquitin-conjugating enzyme 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19 20">ubiquitin carrier protein UBC2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17 21">ubiquitin-protein ligase UBC2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">SPAPADRAFT_68767</name>
    </gene>
    <organism evidence="26">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="23">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="3"/>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377675.1">
        <property type="nucleotide sequence ID" value="XM_007377613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVA6"/>
    <dbReference type="FunCoup" id="G3AVA6">
        <property type="interactions" value="820"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVA6"/>
    <dbReference type="GeneID" id="18875238"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68767"/>
    <dbReference type="eggNOG" id="KOG0419">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_10_2_1"/>
    <dbReference type="InParanoid" id="G3AVA6"/>
    <dbReference type="OMA" id="DHKSQYI"/>
    <dbReference type="OrthoDB" id="9984419at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033503">
        <property type="term" value="C:HULC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990304">
        <property type="term" value="C:MUB1-RAD6-UBR2 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097505">
        <property type="term" value="C:Rad6-Rad18 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990305">
        <property type="term" value="C:RAD6-UBR2 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990303">
        <property type="term" value="C:UBR1-RAD6 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034620">
        <property type="term" value="P:cellular response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071629">
        <property type="term" value="P:cytoplasm protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007535">
        <property type="term" value="P:donor selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042275">
        <property type="term" value="P:error-free postreplication DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031571">
        <property type="term" value="P:mitotic G1 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000639">
        <property type="term" value="P:negative regulation of SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090089">
        <property type="term" value="P:regulation of dipeptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009302">
        <property type="term" value="P:sno(s)RNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120174">
        <property type="term" value="P:stress-induced homeostatically regulated protein degradation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23790">
        <property type="entry name" value="UBCc_UBE2A_2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000007">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0749">Sporulation</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 23" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="24">
        <location>
            <begin position="4"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="22">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00075663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00080892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="EGW29909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="164" mass="18770" checksum="2C8D205053FDCEBB" modified="2011-11-16" version="1">MSTPAKRRLMRDFKRMQQDAPSGVSASPLPDNVMKWNAVIIGPSDTPFEDGTFRLILQFDEQYPNKPPSVKFISDMFHPNVYASGELCLDILQNRWSPTYDVSSILTSIQSLLNDPNISSPANVEAANLYKDHRSQYVKRVRETVENSWLEDDLEDDEDDKDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVE1</accession>
    <name>G3AVE1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_63767</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377925.1">
        <property type="nucleotide sequence ID" value="XM_007377863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVE1"/>
    <dbReference type="GeneID" id="18874905"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63767"/>
    <dbReference type="eggNOG" id="KOG0253">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_52_4_1"/>
    <dbReference type="InParanoid" id="G3AVE1"/>
    <dbReference type="OMA" id="SGWRYLM"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000171">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF4">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="331"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="426"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="460"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="488"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="56"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="517" mass="57179" checksum="16A9B601B130CA74" modified="2011-11-16" version="1">MSDSGNHYDAEKHPEKASIESASSISDGDGELYELSDSVLHQKMKLINDAIDEIGFTPYHLKLFFLNGMGYWTDTQLTFLESSVRTFINYQFGYTFPVSNFMLATGLLFGALFWGLSADLIGRKIAFNFSLMLSAIFTIMTGAMSSMATYCIFVFLSCFSAGGNLVLDTCVFLEYLPHKDQWLLTFFAFFWGIGQVIAVGLAYAFLPNNSCSSIQDCPSHENRGWRYVYYVNGCIVLVMAILRITVIRLKETPKFLISNNRDAEAVELLHNIANKYNRKCSLTLEQLESIGTIEINDDFRKHLDIKGTLQLIAGHVKTLFSNRLVARSSGLVFLSWLLLGFSYPLYSSFLPIYLATRGATISAPDVAGVYRDNLISNSVSTAGPLIAGALLYLFPFLGRRGVLFIGGVCSMAFLFGYTQIKTRAQNVALSSISYASIYIYYAALYAYTPEVFPSVARGTGNALSVACTRVATVVVPLIAYYADTSSSVPIWICGAFVGVLGFLALLFPFEPSKHRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVY0</accession>
    <name>G3AVY0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_69054</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007378007.1">
        <property type="nucleotide sequence ID" value="XM_007377945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVY0"/>
    <dbReference type="STRING" id="619300.G3AVY0"/>
    <dbReference type="GeneID" id="18875252"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_69054"/>
    <dbReference type="eggNOG" id="ENOG502SACR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_341050_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVY0"/>
    <dbReference type="OMA" id="PDINAIN"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012406919" description="Hyphally-regulated cell wall protein N-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="358"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="621"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="648"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="673"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="757"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="779"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="832" mass="87750" checksum="B065154DD58909E2" modified="2011-11-16" version="1">MWIIRLIIFLRMAWCSTKVISGTHQINMPYDDSYSSVFINEDANVVFFGNENICIHKEIDNLGAITFTTKTGTNIESENILSSFNNKGIARFHANHGKAMKMKIYHAIFENSGTILFQSETTTRDIASSTIHGTLGWTNTGTIIINNKSQSPGKFRMGLIVKNSGIIILNNQNYIQSEGQINGGGCIYLEEKSSCELHSVHSSKDQVFVMGEYTSVWIKGILVNNYMFMNFGAQNEIKFDDNLNRNNFQYFDKSGRLQLSLIPYRSIIIGSGYNVKSFQVSQNGKVLSYNGPRPNTTGCDCKKFPFIGQTIPPNGCEDGGSSQFPDINAINQVDIGIIDTDDSDENNVTKVEIPDLIEAGDNSDKVEVPGTDGGGSSDENELELPSVVGGGNDNNKNEVEVSGGTVKGGSENNEVPGPGGSNENEVELVGTIEGSSGNGNKEEENPGGIDEGGNIEEEVPVDYNEVEFPGIAEGGGNSGSNEMELPSIIEGGEGSNKNEVEVPGNDVENGGGNEKNEVESTAGITEGGADKEKEVPDIAEGGNGNKEVENPGGIDEGGNIEEEVPGDYSEVELPGIAEGGGNSDSNEMELPSIIEGGADKEKEVPDIAEGGSNDKEVETPGSNSEGSSNEINNVNDSKDQVDGSGSNEIKDGITEKNGSDRDDGSKSGPQPDGSGSNGTEEGENVSNGDNSGDSSNNDPSSEFNGIPPESNSGSTHVPDGAKGSTSEGNYGGDVLGNGSIDDSDEKKLAETNNGSDGIRETSNETNDKDSNSESKNETPPESNGSIPGSTPGSNSGVSDGMNESNGVNTDGFTDGTPSGPKEWDSRFSCQPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADV4</accession>
    <name>G3ADV4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">RPB2</name>
        <name evidence="24" type="ORF">SPAPADRAFT_53113</name>
    </gene>
    <organism evidence="25">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 26">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="15"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372090.1">
        <property type="nucleotide sequence ID" value="XM_007372028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ADV4"/>
    <dbReference type="FunCoup" id="G3ADV4">
        <property type="interactions" value="1295"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ADV4"/>
    <dbReference type="GeneID" id="18871763"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53113"/>
    <dbReference type="eggNOG" id="KOG0214">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000524_5_1_1"/>
    <dbReference type="InParanoid" id="G3ADV4"/>
    <dbReference type="OMA" id="CYDRNDS"/>
    <dbReference type="OrthoDB" id="10248617at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005721">
        <property type="term" value="C:pericentric heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="C:RNA polymerase II, core complex"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="F:DNA binding"/>
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        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:ribonucleoside binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:siRNA-mediated pericentric heterochromatin formation"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
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        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
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        <property type="entry name" value="RNA_pol_B_RPB2"/>
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        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
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        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
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        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
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        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
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        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
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        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
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        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
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    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
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    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
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    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
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        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
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    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
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        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
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        <property type="entry name" value="RNA_pol_Rpb2_3"/>
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        <property type="entry name" value="RNA_pol_Rpb2_4"/>
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    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
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        <property type="entry name" value="RNA_pol_Rpb2_7"/>
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        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
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    <dbReference type="NCBIfam" id="NF007175">
        <property type="entry name" value="PRK09606.1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RNA_pol_Rpb2_1"/>
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        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="20">
        <location>
            <begin position="43"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="19">
        <location>
            <begin position="222"/>
            <end position="410"/>
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    <feature type="domain" description="RNA polymerase Rpb2" evidence="21">
        <location>
            <begin position="484"/>
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            <begin position="583"/>
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            <begin position="681"/>
            <end position="756"/>
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    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="17">
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            <begin position="764"/>
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            <begin position="1137"/>
            <end position="1229"/>
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    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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            <dbReference type="EMBL" id="EGW34678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1238" mass="140760" checksum="67C7175933B8CD7D" modified="2011-11-16" version="1">MSTQNQHLHEEEDDPYMYDDGDSQSITPEDCWTVISSFFQEKGLVSQQLDSFDEFIETTIQELVWEDSHLILDQPAQHTSESDHENRRYEITFGKIYISKPSQTEGDGTTHPMFPQEARLRNLTYSSPLYVDMTKKVFKSDDNKRKENNELDWIEEKVEDEDPRTKVYLGKVPIMLRSKFCMLRDLGEHDFYELKECPYDMGGYFVINGSEKVLIAQERSAANIVQVFKKAAPSPISHVAEIRSAIEKGSRLISSMQIKLYGRDEKGVSARTIKATLPYIKEDIPIVIVFRALGVVPDGDILEHICYDANDWQMLEMLKPCVEEGFVIQEREVALDFIGRRGVLGIRREKRIQYAKDILQKELLPNITQEEGFETRKAFFLGYMVNRLLLCALERKEPDDRDHFGKKRLDLAGPLLANLFRILFKKLTKDIYNYMQRCVENDKEFNLTLAVKSQTITDGLRYSLATGNWGEQKKAMSSRAGVSQVLNRYTYSSTLSHLRRTNTPIGRDGKIAKPRQLHNTHWGLVCPAETPEGQACGLVKNLSLMTCISVGTPSEPILYFLEEWGMESLEDYVPSNSPDSTRVFVNGVWVGTHRDPVNLVDTMRSLRRRGDISPEVSIIRDIREKEFKIFTDAGRVYRPLFIVDDDSESETKGDLKLQKEHVLKLLNSEYDDYDGDEGYNWTSLVNDGIVEYVDAEEEETIMIAMTPEDLEASKNTLSQTQIKAMQVEEQELDPAKRIKPVFSSATHTFTHCEIHPSMILGVAASIIPFPDHNQSPRNTYQSAMGKQAMGVFLTNYSVRMDTMANILYYPQKPLATTRSMEFLKFRELPAGQNAIVAIACYSGYNQEDSMIMNQSSIDRGLFRSLFFRSYMDLEKRQGMKALETFEKPSRSDTLRLKHGTYEKLDDDGLIAPGVRVSGEDIIIGKTTPIPPDTEELGQRTQYHTKRDASTPLRSTESGIVDQVLLTTNGDGAKFVKVRMRTTKVPQIGDKFASRHGQKGTIGVTYRHEDMPFTAQGIVPDLIINPHAIPSRMTVAHLIECLLSKVSALSGLEGDASPFTDVTAEAVSRLLREHGYQSRGFEVMYNGHTGKKLMAQVFFGPTYYQRLRHMVDDKIHARARGPVQVLTRQPVEGRSRDGGLRFGEMERDCMIAHGAAGFLKERLMEASDAFRVHVCGSCGLMSVIANLKKNQFECRSCKNKTNIYQIHIPYAAKLLFQELMAMNISPRMYTERSGQTVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADV5</accession>
    <name>G3ADV5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL13m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_131310</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372091.1">
        <property type="nucleotide sequence ID" value="XM_007372029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ADV5"/>
    <dbReference type="FunCoup" id="G3ADV5">
        <property type="interactions" value="765"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ADV5"/>
    <dbReference type="GeneID" id="18869562"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131310"/>
    <dbReference type="eggNOG" id="KOG3203">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082184_1_1_1"/>
    <dbReference type="InParanoid" id="G3ADV5"/>
    <dbReference type="OMA" id="HKPIYTP"/>
    <dbReference type="OrthoDB" id="274622at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="50S ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005823">
        <property type="entry name" value="Ribosomal_uL13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_uL13_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01066">
        <property type="entry name" value="rplM_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="174" mass="19919" checksum="AE534DBDF31F6FE8" modified="2011-11-16" version="1">MSQRIGHSVSALTKVWHHVDVATEERTLGRLAASIAQTLIGKHKPTYSPNRDHGDYVVVTNCSHLYVTGKKMQQKHYWSHTSRPGSLNLISMERMVANKGYGEILRRAVKGMIPKNKHRLVRMDRLKVYDGDEHPYKDNLIAFADEVPEMLAKAKKLEENEATRAELRKKYLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADW1</accession>
    <name>G3ADW1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Superoxide dismutase</fullName>
            <ecNumber evidence="3 10">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_57734</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="3 10"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372097.1">
        <property type="nucleotide sequence ID" value="XM_007372035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ADW1"/>
    <dbReference type="FunCoup" id="G3ADW1">
        <property type="interactions" value="632"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ADW1"/>
    <dbReference type="GeneID" id="18872174"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57734"/>
    <dbReference type="eggNOG" id="KOG0876">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031625_2_1_1"/>
    <dbReference type="InParanoid" id="G3ADW1"/>
    <dbReference type="OMA" id="DSLINWD"/>
    <dbReference type="OrthoDB" id="239262at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.990:FF:000001">
        <property type="entry name" value="Superoxide dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.55.40.20:FF:000002">
        <property type="entry name" value="Superoxide dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050265">
        <property type="entry name" value="Fe/Mn_Superoxide_Dismutase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001189">
        <property type="entry name" value="Mn/Fe_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019833">
        <property type="entry name" value="Mn/Fe_SOD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11404">
        <property type="entry name" value="SUPEROXIDE DISMUTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11404:SF6">
        <property type="entry name" value="SUPEROXIDE DISMUTASE [MN], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00081">
        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000349">
        <property type="entry name" value="SODismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01703">
        <property type="entry name" value="MNSODISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00088">
        <property type="entry name" value="SOD_MN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase N-terminal" evidence="11">
        <location>
            <begin position="31"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="12">
        <location>
            <begin position="124"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000349-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="228" mass="25056" checksum="F4F2ECC8AEA903FB" modified="2011-11-16" version="1">MLSLRTASRALSKSAPIAAAASIGAVRTKVSLPDLDWDFGALEPHISGQINEIHYTKHHQTYVNNYNVATEQIAEASAKGEVKKIVELQKAINFNGGGYINHCLFWKNLAPEKQGGGEPPTAESDFAKKVVEQYGSLDNLKALTNAKLAGIQGSGWAFIVKNKENGGALDVVTTPNQDTVTGPLVPLVAIDAWEHAYYLQYQNVKADYFKAIWNVINWKEAEKRYLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADY8</accession>
    <name>G3ADY8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_57774</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372124.1">
        <property type="nucleotide sequence ID" value="XM_007372062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ADY8"/>
    <dbReference type="STRING" id="619300.G3ADY8"/>
    <dbReference type="GeneID" id="18872190"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57774"/>
    <dbReference type="eggNOG" id="ENOG502SAYY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022377_0_0_1"/>
    <dbReference type="InParanoid" id="G3ADY8"/>
    <dbReference type="OMA" id="FPACKYL"/>
    <dbReference type="OrthoDB" id="4937900at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="542" mass="63918" checksum="03A4924D9E66C5CD" modified="2011-11-16" version="1">MSDEISRDQSPQQQFQSPKQQYQSPKMLPNGIKQTMHFTSIISRFKRKITLEDVNARAVADLTHHGVLGKVNLLDLLSDMILELPKKKDSDVLAQAYVCLGEAIILNIFRRRQKYMTNFLYTNSINSLNQSCLERHGYCESRIRSLFVDLSTKKNFLTHDEMSNLSEVLAYSSYLMTFAVLMMKFGAQSYFHSCKGVITTFEEYTIYMMERNLQPISTVSFLMNNLQYNIVSINIPSYNPQFIFEIESNVRCLDFIFNNSFTFQDESISLRFQLILLRDENYVTTYPPVVIYEIFKEWHTIFPSEAMTYTLFKNDAHSVEAVFLNALSTTFYMYYFAVAASLDALFPACKYLYGMSFMVPTNKFFIKREIMALDKENPYIQGLLRFDCRLQGHIYYASRVFAFFRRRYVFYHNNVKWSNPYDDLTDETRFETRVIKNTEIPIKSFNTTLIRPEHYPTTKGSVNFNSLTFTREDESMMHNAYSRNIETLNFDETFVLQYDCETMLLLRDYRPPDITNNDRPRLEIKDINQYYEDRTKILNSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADZ0</accession>
    <name>G3ADZ0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_57777</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. LCMT family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372126.1">
        <property type="nucleotide sequence ID" value="XM_007372064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ADZ0"/>
    <dbReference type="FunCoup" id="G3ADZ0">
        <property type="interactions" value="108"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ADZ0"/>
    <dbReference type="GeneID" id="18872192"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57777"/>
    <dbReference type="eggNOG" id="KOG2918">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002761_0_0_1"/>
    <dbReference type="InParanoid" id="G3ADZ0"/>
    <dbReference type="OMA" id="FCILEQF"/>
    <dbReference type="OrthoDB" id="47172at2759"/>
    <dbReference type="UniPathway" id="UPA00375"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46529">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46529:SF1">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13418">
        <property type="entry name" value="Beta-prop_TYW4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="464" mass="52875" checksum="9B388DAD2F0CB35F" modified="2011-11-16" version="1">MKPEFANPVIEISSKVSNSHFLILEQIMPDSSDNSFATKMLYHFNHLRSPIQCVEHYPTKELQMTRFRQYYSSVEVKNLFENWLYLVDDEMKSKISQVEEFDEWEEFIIFCQHYVLVHGTNTDQLIYETPNGEIESKEYPMDTTVTMVQDSRFNAEQLEIKFPAITSVDSKIYVNGGLKQTRTNEMLELDLESGTISNVEMNLQPSARMCHTLTTLADNKMLLVGGRSRPGLHFQDVYLCNKGVWEKLPDMPVKRSRHACVSVTEAEVLLFGGLTDENNDSDKLFLQYDVRSGTSKELKIKGDSPGNLLSCSMNYDGEFGYIFGGISNHNVPIVNDKLYKFKIIDDTIEIESVYQDYLLSRIGSQSKLLGNKLLIVGGVSTIKMLTKKTNIMTLELADFKWKYVQVPEQIRKTSPPIFIGFGLVEKSSHKQRDASASYFMLGGGAVCYSFGSCFNSVYRLDIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE10</accession>
    <name>G3AE10_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein eL24-related N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_58776</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372956.1">
        <property type="nucleotide sequence ID" value="XM_007372894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE10"/>
    <dbReference type="FunCoup" id="G3AE10">
        <property type="interactions" value="909"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AE10"/>
    <dbReference type="GeneID" id="18872600"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58776"/>
    <dbReference type="eggNOG" id="KOG1722">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106411_0_0_1"/>
    <dbReference type="InParanoid" id="G3AE10"/>
    <dbReference type="OMA" id="PGHGKKM"/>
    <dbReference type="OrthoDB" id="1727108at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.20:FF:000002">
        <property type="entry name" value="60S ribosomal protein L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.10.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056366">
        <property type="entry name" value="Ribosomal_eL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023442">
        <property type="entry name" value="Ribosomal_eL24_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01073">
        <property type="entry name" value="RIBOSOMAL_L24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL24-related N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="155" mass="17480" checksum="CD403E1EF42FD7AB" modified="2011-11-16" version="1">MKIEIDSFSGSKIYPGRGTLFVRGDSKVFRFQSSKSASLFHQRKNPRRISWTVLYRRHHKKGISEEAAKKRTRKTVKHQRAIVGASLELIKERRSQKPSDRKAAREAKLVKDKEAKKVAKAARKAEKAKLAASGANVVSKQQAKGAFQKVHATSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE24</accession>
    <name>G3AE24_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein NACB1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NACB1</name>
        <name evidence="8" type="ORF">SPAPADRAFT_53773</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372970.1">
        <property type="nucleotide sequence ID" value="XM_007372908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE24"/>
    <dbReference type="GeneID" id="18871811"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53773"/>
    <dbReference type="eggNOG" id="ENOG502RJRW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008511_1_1_1"/>
    <dbReference type="InParanoid" id="G3AE24"/>
    <dbReference type="OMA" id="LLTVQCF"/>
    <dbReference type="OrthoDB" id="3364175at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000435">
        <property type="term" value="P:positive regulation of transcription from RNA polymerase II promoter by galactose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051127">
        <property type="entry name" value="Fungal_SecMet_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF3">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="19"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="655" mass="74575" checksum="C5A603B45B7747C6" modified="2011-11-16" version="1">MNEKVSKDTKSKRPRVVLACNRCRQRKTKCDGQQPVCGGCMKKQVECSYGVKYNRAHVTVEYVRQLEEKLGIAPDSTDAMGAGSMDKKRRGKDRDYYGSSAAISFMKDISNVIDGSYRSELGSSTTTEDERDTEKSNVYRMSRNDAAVSRRTTDIVLPPRAMADRYVKNYFEYAYTLYPFVHQPTFMATYQTIWSNNDDDNEIDELFYSIVNIIFAFGCQLTPEPEKLEDNSELYFERSQELLRFHLMDTGSILLVQALLLTGQFLQASTGSAGCWNIVGLSIRIAQGLGLHSEQNYASAKSLIDREIRKRLWQGCLLMDRIVSMTMGRPLMVTQDNESGELPLCVDDEYIKDDAILPMKTPSKIGFFIQTIKLYNILADILKQVYPNEIQDDVVQLLLNIFNFENRLNEFRANLPEYLQYKSDERKMMPFERQSIVLHIRTLHMKIMLYRPTLFPKSSRNHTHTSELYTSTQKSVAISCVKLAVELIDLIGRYRAGDIIYLPAHWYNVFYIYTAANVLLAAKLQVGLHRDLDLATFENAWNHSLEILGSYTQHSESASRCLKVLEMMGNKVNISSQRLATQRNTPVPHLHHDSDHLHPASTAVPGSVYPLHNYSPSSTSSEVPTDVLYSLLYDTAGPFGGPFHYSDDPNVLGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE35</accession>
    <name>G3AE35_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase PEP5</fullName>
            <ecNumber evidence="9">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_69768</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex. Component of the class C core vacuole/endosome tethering (CORVET) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Vacuole membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the VPS11 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="9"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372981.1">
        <property type="nucleotide sequence ID" value="XM_007372919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE35"/>
    <dbReference type="FunCoup" id="G3AE35">
        <property type="interactions" value="609"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AE35"/>
    <dbReference type="GeneID" id="18875289"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_69768"/>
    <dbReference type="eggNOG" id="KOG2114">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001287_0_0_1"/>
    <dbReference type="InParanoid" id="G3AE35"/>
    <dbReference type="OMA" id="ENENECP"/>
    <dbReference type="OrthoDB" id="26184at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033263">
        <property type="term" value="C:CORVET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036205">
        <property type="term" value="P:histone catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035542">
        <property type="term" value="P:regulation of SNARE complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032889">
        <property type="term" value="P:regulation of vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099022">
        <property type="term" value="P:vesicle tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16688">
        <property type="entry name" value="RING-H2_Vps11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057308">
        <property type="entry name" value="CHCR_PEP5_VPS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057307">
        <property type="entry name" value="PEP5_VPS11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016528">
        <property type="entry name" value="VPS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024763">
        <property type="entry name" value="VPS11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323:SF24">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23341">
        <property type="entry name" value="PEP5_VPS11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23356">
        <property type="entry name" value="TPR_PEP5_VPS11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12451">
        <property type="entry name" value="VPS11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17122">
        <property type="entry name" value="zf-C3H2C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007860">
        <property type="entry name" value="VPS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0926">Vacuole</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="904"/>
            <end position="954"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR007860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW35569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1010" mass="115207" checksum="CE39507518490D80" modified="2011-11-16" version="1">MSSISSLLSSWRQFQLFDFTPIRDPNYQTSDALYSDPTISAINATTTNYLIIAINNSTLKIINPKDLTTLGVFTAYDVDYRITFIAPLAHSDNLVVTLAEKQGAPSIIKLWDIHKVLNLQDVDDSEFQFKFQTQVLVSNGDNSFPISCFVFNNDLTCLAIGYTNGKVILIRGDLLRDRGAKQRVIYESNDPITGIQFNEKEAVLYITTTSKILTVATTGRNHGKPARVLSSKTGADLNCTAVDQSTQSLIVGLPGSIRYYNHLQSLSSINFDIAKSKIISSKSSYLLIVSPQHEDVTSKALMTRIIILDLVNKHISFNIIIPNSLINYAFISSGDLYLLSGDGVLYKLHEKPINQQIELVLQRELFQVAFNLAKQSNLPSKVLLRIQRLHGDYLYDSQRYDESIDVFIDCLDLFEKTQPEAKLNGDKQKGDDQEVVEEDEEDLDDFIMNIITKFKEATNISNLTRFLMKLYEKSIANIDHITLLLCCFCKLKQVESLDDFIESLVITTTNLQDLNFELIINLFKECGYFHQVIKLLFKLEQPGLIVDIQLNDLKKPKLALSYMKSLAIDELLLILIDHSKKLLDSCPIETTELLINVFTGKYKPQNTEEGPIELDKGGEKEEPEGVAKLTNYRAFVNYLSGGSDEQEQEEDDLSDEPTYLPPRPNLIYSSFMNHPNEFLIFLEACIEAFDKFQGNEVDKRETLLTLLEMYLSIYETTKAKEWIDKAEALTTHYASLLDKKSLLLISHIYNFKQGEVIAKEEAGMEESLFINYQIAEDVPGCMELLNKYGSSKPELYKLMLKFIVSRKSIFERVNQKDFQYILEKIKYYKLMNPLEVLQVITEQPENDFITLGVVKQYLIDHFKEKSKEIVNNEKLIEKYEQESTKNSFKLSELTKPFVIQNNKCSMCELKLDFPVIHFKCRHSFHQKCLSTNLVASNDEPVIDGKGEVPQCPICISHVNEVKAVKAGHFRAKENYDAFESSLHDSNDKFKFVTDYFGKGVMENESITMVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEC9</accession>
    <name>G3AEC9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Aspartokinase</fullName>
            <ecNumber evidence="4 13">2.7.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_58914</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Phosphorylates aspartate, the first step in the biosynthesis of amino acids that derive from aspartate (the aspartate family of amino acids), including methioinine and threonine, the latter of which is a precursor to isoleucine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-aspartate + ATP = 4-phospho-L-aspartate + ADP</text>
            <dbReference type="Rhea" id="RHEA:23776"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:23777"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the aspartokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.4" evidence="4 13"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373129.1">
        <property type="nucleotide sequence ID" value="XM_007373067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEC9"/>
    <dbReference type="FunCoup" id="G3AEC9">
        <property type="interactions" value="326"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEC9"/>
    <dbReference type="GeneID" id="18872678"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58914"/>
    <dbReference type="eggNOG" id="KOG0456">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_6_4_1"/>
    <dbReference type="InParanoid" id="G3AEC9"/>
    <dbReference type="OMA" id="DNINIMM"/>
    <dbReference type="OrthoDB" id="4323675at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004072">
        <property type="term" value="F:aspartate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071266">
        <property type="term" value="P:'de novo' L-methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009090">
        <property type="term" value="P:homoserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2130.10:FF:000001">
        <property type="entry name" value="Bifunctional aspartokinase/homoserine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000023">
        <property type="entry name" value="Probable aspartokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054352">
        <property type="entry name" value="ACT_Aspartokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001341">
        <property type="entry name" value="Asp_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018042">
        <property type="entry name" value="Aspartate_kinase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00657">
        <property type="entry name" value="asp_kinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21499">
        <property type="entry name" value="ASPARTATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21499:SF59">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22468">
        <property type="entry name" value="ACT_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00324">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 13 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0791">Threonine biosynthesis</keyword>
    <keyword evidence="6 13 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="14">
        <location>
            <begin position="458"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW35717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="540" mass="59422" checksum="82D81290C535EB40" modified="2011-11-16" version="1">MSDSPPLSARSYNSVLDFKQTPPSTPLTTGGWIVQKFGGTSVGKFPENIVDDIVKVFSENNRVIVVCSARSSHTKSEGTTSRLLRTADLASSKGDFLSLLQVIEDDHVDNAKNKIESEEIRQKLIEDTKTELNHIKELLHACQVIGEISPRSLDTIMGVGEKLSCLFIAALMKDHGLNSVYMDLSDLIPLDYDFNNGFDDTFYKFLSSELGRRVAELEDDDAIPVMTGYFGVVPGGLLNGVGRGYTDLCAALAAVGVQADELQIWKEVDGIFTADPRKVPNARRLDSVTPEEAAELTYYGSEVIHPFTMEQVIKAKIPIRIKNVENPTGKGTIIFPDNIGRRGEATPPHPPAAYETLPSSYISSHKKRSATAITAKQNIVVINIHSNKKTLAHGFLAKIFTTLDKHKLVVDLISTSEVHVSMALSIQAEQELQLKHSLQELKTMGTVDITREMTIVSLVGKQMVNFIGIAGNMFKILADEKINIEMISQGANEINISAVINEKDTIRALQSIHAKLLEDNYEDTAASAIDLRLQFLKTQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEG4</accession>
    <name>G3AEG4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_132131</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373164.1">
        <property type="nucleotide sequence ID" value="XM_007373102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEG4"/>
    <dbReference type="STRING" id="619300.G3AEG4"/>
    <dbReference type="GeneID" id="18869638"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132131"/>
    <dbReference type="eggNOG" id="ENOG502S1P2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082765_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEG4"/>
    <dbReference type="OMA" id="GASWWGF"/>
    <dbReference type="OrthoDB" id="10005898at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652:SF25">
        <property type="entry name" value="MICROBODY (PEROXISOME) PROLIFERATION PROTEIN PEROXIN 11C (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="302" mass="35135" checksum="5BD2F2840F27D331" modified="2011-11-16" version="1">MQKQASGSRKNRIGSVPITTLLQLQSLLKKSVFSRKFYQEINDKVLASSATVDANLYFICYFTLLVSAVLNNRPKIVYWLQKQKYNLLQVIKKFSQLQFGTDLSQSDNKLVSKIFSQQPPVLDEKASSSLAVHFKAISSYLSDIRIFNRLTDSIKYMPWIIDEFHAYMDPSNPTAKFDRLVNFVQSLNCLVLELLENAGWLTDHNWVGTGDNDWWSFETYIWCSRIWGAYLLIEIIELVRRTPRSKWNTKWKISLFQQVIQVPLVAHWSLREGCLTPFWVGVCGCGASWWKFKDMWKSLDMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEI0</accession>
    <name>G3AEI0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Autophagy-related protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Autophagy-related E2-like conjugation enzyme ATG3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_57808</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt) and autophagy. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8. The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8 from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8. The formation of the ATG8-phosphatidylethanolamine conjugate is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). The ATG8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATG3 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372154.1">
        <property type="nucleotide sequence ID" value="XM_007372092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEI0"/>
    <dbReference type="FunCoup" id="G3AEI0">
        <property type="interactions" value="1092"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEI0"/>
    <dbReference type="GeneID" id="18872203"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57808"/>
    <dbReference type="eggNOG" id="KOG2981">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027518_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEI0"/>
    <dbReference type="OMA" id="KWCKAIS"/>
    <dbReference type="OrthoDB" id="1584384at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061908">
        <property type="term" value="C:phagophore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019776">
        <property type="term" value="F:Atg8-family ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061723">
        <property type="term" value="P:glycophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007135">
        <property type="entry name" value="Atg3/Atg10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12866">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12866:SF2">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03987">
        <property type="entry name" value="Autophagy_act_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="97"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="130"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="267" mass="30482" checksum="6132786B4CAA9BC3" modified="2011-11-16" version="1" fragment="single">MLRSKLSSLREYLTPINHKSNFSTTGEISPEEFVEAGDYLVFKFPTWRWSSCPKNLQKSFLPEEKQFLITKHVPSYQRASTYLNGGSDSIDPDLLEEEEEIEGEEGEDGWVKSRKVATSNKTAEAKQEDKESEDELEEAVEVNDIDELIDVTAEGGEEEEEEDDFDDLDIINDSNNKLRKYDMYITYSTSYRVPKMYLVGFDSNGIPLLPKQMFEDINSDYVDKTATIENLPVAHNTTSVSIHPCRHASVMKVLMKHSKLKKASDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEP0</accession>
    <name>G3AEP0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Myosin-5 isoform</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">SPAPADRAFT_130687</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Actin patch</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373078.1">
        <property type="nucleotide sequence ID" value="XM_007373016.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AEP0">
        <property type="interactions" value="355"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEP0"/>
    <dbReference type="GeneID" id="18869515"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_130687"/>
    <dbReference type="eggNOG" id="KOG0162">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000192_7_6_1"/>
    <dbReference type="InParanoid" id="G3AEP0"/>
    <dbReference type="OMA" id="NDQENQC"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007121">
        <property type="term" value="P:bipolar cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071852">
        <property type="term" value="P:fungal-type cell wall organization or biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01378">
        <property type="entry name" value="MYSc_Myo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11858">
        <property type="entry name" value="SH3_Myosin-I_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000015">
        <property type="entry name" value="Myosin I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000004">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000007">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035535">
        <property type="entry name" value="Fungal_myosin-I_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054489">
        <property type="entry name" value="Myo1_CA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036072">
        <property type="entry name" value="MYSc_Myo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF837">
        <property type="entry name" value="MYOSIN-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22773">
        <property type="entry name" value="Myo1_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 15" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10 15" id="KW-0518">Myosin</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 14" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Myosin motor" evidence="18">
        <location>
            <begin position="40"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="TH1" evidence="19">
        <location>
            <begin position="785"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="1132"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="15">
        <location>
            <begin position="600"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="969"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1048"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1191"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1270"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="969"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1004"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1048"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1071"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="1113"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="1199"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1209"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1288"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="133"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EGW35666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1298" mass="145171" checksum="394E32700643BE13" modified="2011-11-16" version="1">MAIVKLGGRKKNQAPPAKSNGSGGGGIKKAEFDITKKKEVGVSDLTLLSKITDEAINDNLHKRFMNDTIYTYIGHVLISVNPFRDLGIYTPEHLDKYRGRNRLEVPPHVFAIAESMYYHLKSYGENQCVIISGESGAGKTEAAKQIMQYIANVSVDKGNVEITKIKDMVLATNPLLESFGCAKTLRNNNSSRHGKYLEIQFSEGSYQPIAAHITNYLLEKQRVVSQITNERNFHIFYQFTKACPEKYKQQFGIQGPESYIYTAAAKCIDVDGINDSKDFNDTINAMNIIGLTQEEQDNIFRMLAAILWVGNISFVEDESGNAAIRDETVTQFVAYLLDVSPEIMKKAIIERTIETTHGSRRGSTYHVPLNIVQATSVRDALAKGIYNNLFEWIVERVNVSLKGVQQSDKTIGILDIYGFEIFEHNSFEQVCINYVNEKLQQIFIQLTLKAEQDEYVQEQIKWTPIEYFNNKVVCDLIEEVRPNPGLFAALNDSIKTAHADSEAADQVFAQRLSMVGASNRHFEDRRGKFIIKHYAGDVTYDVAGMTDKNKDAMLRDLLELLGTSSNQFITQILFPPGLLAALTDSKKRLETASDKIKTSANKLVDTLSKCSPSYIRTIKPNQSKKPRDYDNHQVLHQIKYLGLKENVRIRRAGFAYRSTFERFVQRFYLLSPQTGYAGDYIWRGDDISAVKEILRACYIPATEFQMGTSKVFIKTPETLFALEDMRDKYWHNMATRIQRAWRRYVKRKEDAAKTIQRAWRVKKHGNQFEQLRDYGNGLLQGRKERRRMSMLGSRAFMGDYLGCNFSSGYGRFIVNQIGLKEQVIFSAKGEILTAKFGRSSKRMPRIFILTRSNLYIIAEVLVEKRLQLQTELTIPLGGINYVALSTLQDNWVAVSLHSFTPTTHDVLINLDFKTELITHMKKISPGLTIKVGPTVEYQKKAGKYHSVKFTIGSGAEVPVNSDHYKSGTVTVRQGLPANSKNPKRPRGRSGKVDYSKYYNRGAATRSSVSKPVIQRQPTHQFQQQAPVYQTPAYDTQVQQQQQQPVVQQQPVVQQQTSPSQQRRKVPPPAPVNVAAAAAQAAYGQKPQTVQPAAQAKAQTPTPVVPSRAKKTAPAPPAKKTAPPPPPPALSKPKHPTYKAMYDYDGSVPNTFPLTKDEVYYVEEKNDNWALVKKLDESAKGWSPVAYLQECAPPTGKASAPPPPPPPPAQAAQPSATTTTATDYSNPTSSTSSYTTQTTAATTAAASDGAANLGNGLADALKQRKNEETTLAGSLADALKRRQGATRDDSDEEEDDDDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEV8</accession>
    <name>G3AEV8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Centrosomin N-terminal motif 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_48750</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373200.1">
        <property type="nucleotide sequence ID" value="XM_007373138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEV8"/>
    <dbReference type="STRING" id="619300.G3AEV8"/>
    <dbReference type="GeneID" id="18871259"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48750"/>
    <dbReference type="eggNOG" id="KOG1836">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011000_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEV8"/>
    <dbReference type="OMA" id="MFVESAQ"/>
    <dbReference type="OrthoDB" id="10255522at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.310.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012943">
        <property type="entry name" value="Cnn_1N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43941">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43941:SF1">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07989">
        <property type="entry name" value="Cnn_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Centrosomin N-terminal motif 1" evidence="5">
        <location>
            <begin position="108"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="504"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="757"/>
            <end position="876"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1015" mass="119618" checksum="2A4E582B19D17957" modified="2011-11-16" version="1">MAYDGLLVGTPKRSSIFGRGSRAQEFTPIGEKRHSGFQVIHNYPQNEAPKLFKPIVQNSPRRDIEEEFSQSFYDDDTIDKFDHNSPIKTNVKYDSGKPKSFVNDGGISIKESEERIKQLSAENFSWRLKFMELKKYLDSVPKTDRELMLENVDLKHAIVELEKRLESGTHVRSDNSEYERIIRDKDNQIHSLGNQIHEMEQDILELEDKYRTQQADLDHQQEKFDRLQSSHQNEIVHQQEKLERLQSTQADSARRSQSESNELAEAQHEIQELRNLLDLHNSEMKQLNEDKIEAETKFKKLQQEMMNELEDLHDTHKRDRQEIARLEQEIVRLSEQQQRFNTNDNQLRQERNQLLNQEKDYSSKLSDLQRQLRDKLELEQVLKSKSADLERKLSKKSIEVSNLEQQLSEKTKEVMNLETKIGYLNSKSKGETVLQTQLQQKDIDIDRLETKLKQLEHSTSQEQDQLRELELERGHLVMQLESKDNQLSSLKSQLGKSSDDQDKIRFYEEEYEAIKLELEDKQREVLNYKTRLGKLEQERNKLADRLESDETKFRSSDIELSQLKADNENLERVNRTLKDKLDTLKHELNTFSTSTRDLSKLQIENRKLNEQITTLDTKLSEFQIENRKLQAELDNVSSSNRVLKSEYETLNQMMNSSRSTNSQLDELLQDNRELVERLETANRTAESRSREIRKLELLVDDYKSRLTSRFDSNDYIEDLVREISQLKDKLHEREVYIDRLLGDFDRTRDDPMLESKLKNAQTLIDNYERKIKILEDEILSRERSATRDESPIEAVLELKVQAANEKIDTLSKQISDLRKKQDSDKSIIHKLENEKNLLEDDLFLVRSQQKEISREKERLAGQVSNLDEELERMTRSCRLMSLKAHDYKQQIMKKGDANYEDKFNILEKEAIYYKAKLRDVMMKANDFRMMNTFIMKSITGNTSMYDGKMNLAKLGIYPEYVATYKRKEKLTFKGVAKFVLAAIRLKNRTKKSKVRSKDLKALKSEIECGRIQLDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEW2</accession>
    <name>G3AEW2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyruvate dehydrogenase E1 component subunit alpha</fullName>
            <ecNumber evidence="5">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PDA1</name>
        <name evidence="7" type="ORF">SPAPADRAFT_59001</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="5"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373204.1">
        <property type="nucleotide sequence ID" value="XM_007373142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEW2"/>
    <dbReference type="FunCoup" id="G3AEW2">
        <property type="interactions" value="645"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEW2"/>
    <dbReference type="GeneID" id="18872714"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59001"/>
    <dbReference type="eggNOG" id="KOG0225">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029393_5_2_1"/>
    <dbReference type="InParanoid" id="G3AEW2"/>
    <dbReference type="OMA" id="SKRDPIM"/>
    <dbReference type="OrthoDB" id="10256198at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000013">
        <property type="entry name" value="Pyruvate dehydrogenase E1 component subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050642">
        <property type="entry name" value="PDH_E1_Alpha_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017597">
        <property type="entry name" value="Pyrv_DH_E1_asu_subgrp-y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03182">
        <property type="entry name" value="PDH_E1_alph_y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF60">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5 7" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="6">
        <location>
            <begin position="66"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="400" mass="43927" checksum="121AFBF596DF057B" modified="2011-11-16" version="1">MLRYSPVHRQLAGAAAKVMARRTMASGNTDLVTVTLPESSFEGYNLDVPSLEFETEKESLLKMYKDMVIIRRMEMAADALYKAKKIRGFCHLSVGQEAIAVGIENAITPKDTVITSYRCHGFAFMRGASVKSVLAELMGRRSGISHGKGGSMHMFAPGFYGGNGIVGAQVPLGAGLAFSHKYRGDGCASFTLYGDGASNQGQVFEAYNMAKLWNLPCIFACENNKYGMGTAAARSSAMTEYYKRGQYIPGLKVNGMDVLATYQASKFAKDWAAQGNGPLVLEYETYRYGGHSMSDPGTTYRTREEVSQMRSRNDPIAGLKATLLEKGIATEKEIKAYDKEARAYVDEQVAAAEADAPPEAKMDILFEDVYIPGSEIPTLRGRISDDTWDFKNKTFLNKVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFC8</accession>
    <name>G3AFC8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TEL2-interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_145555</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372327.1">
        <property type="nucleotide sequence ID" value="XM_007372265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFC8"/>
    <dbReference type="FunCoup" id="G3AFC8">
        <property type="interactions" value="625"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFC8"/>
    <dbReference type="GeneID" id="18870690"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_145555"/>
    <dbReference type="eggNOG" id="KOG4524">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005544_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFC8"/>
    <dbReference type="OMA" id="SLYKPWG"/>
    <dbReference type="OrthoDB" id="6781668at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052587">
        <property type="entry name" value="TELO2-interacting_protein_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057567">
        <property type="entry name" value="TPR_TTI1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057566">
        <property type="entry name" value="TPR_TTI1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049362">
        <property type="entry name" value="TTI1_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460">
        <property type="entry name" value="TEL2 INTERACTING PROTEIN 1 TTI1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460:SF3">
        <property type="entry name" value="TELO2-INTERACTING PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24181">
        <property type="entry name" value="TPR_TTI1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24173">
        <property type="entry name" value="TPR_TTI1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21547">
        <property type="entry name" value="TTI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TTI1 N-terminal TPR" evidence="2">
        <location>
            <begin position="21"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="TTI1 C-terminal TPR" evidence="3">
        <location>
            <begin position="850"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="922"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="927"/>
            <end position="949"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1167" mass="132363" checksum="9F467B6A9041FD16" modified="2011-11-16" version="1">MLPLRSGIGEDAGKLLAQSLFSKIKPICVELSQVLNAPEFNINKVRSLLTSLEAALYKHLKEAQDQVVIYRLPETVADYIFFPISNLLKFPRLDDSITQHILAIIGFLIDNAWSFNVNYNLIDQLFPLLVYLSGFDTKAQETSLSEKSLQFKSSSISALFSISKTLTRSYFQGEGKRLVFLSNSISIVIDIIGSTTPNSQEAIKVLEIIFDTILQLVQCLTSSQQSQILPGIVSSVTTFGSKNSNIPAELTIRILKVLSFIICNSFNDNELHAELDVHHIKNLKDLEVVWNTNEVATTERCNITITETDHRSLSWVKATSKQLKISLTVFFKTILHSEKNKQRLKSKPELYNEVINFVSGILNSCFATLFTEFASLSIDILSSLVYASSYGEEKDINDKISSISNMLCLCLDDDSKAMAFFEILHSKLLYLLQNKLTSTLFSIDEEKVSMTIASLKLNFQLLFDLSRSQSTDYQVLNELRKFTLKLFRQHMLDRFNLENSKSASKIGNSDTLIPLNEGESSNKLDSIELPEYVNVHNIATQNKTLTKSTSSYIHNLQLISRNWNEASILANSEESSTLGIGTKLIEFKVAGFIQFLSNLRFRNDSDSTLNDLEDIFMDCDDNSLETSLSLWIATRLIKNYTSSKSIEEHEFDPDKFLDFTETEEESSYIDEELDAGEESLYLVLAKSEELISSVSSKDDGELNVSDELIYCCALDAINELAGKLSKEQFQSDFLMDNLLVLIKSLTYNNRPRVQQVAQNTVKSILEAYYDNSMKKLIIENSDYLIDSLSLQMSVASNLTPVLPGILVIIIKITGIQLLESNQLTDILTEMFVLLDTYHGYNKLAESFFVVFESLTEQINKQYGSRQTIKSADNINSSSYKPWGMSSKEQMMALISGAGSVLDSVVEYDSTKEYFKRKTDVPFSEISQDSDDEDESEEEQEEEEEEEEEWQSVVPKKVYFILQRIFNYGFVLIAQPSFSLRAQIIKTLRLIFPLLCFNYKLVLPVIASNWPLLVTLISGSKSLSTYPNIDVIGYTSEETNLMFQSLEFVTEILTEDVSKEEYFFARKFQEAWEFMSEHSKLISSKGTKDELSKERSLTVSQSPLRTHPKLRNVLIKFLITGVQAYERTIPDIVRYNVVSLCHRLKIPEDLILSRDTAGIIEVLQSHEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFF7</accession>
    <name>G3AFF7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_48009</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372358.1">
        <property type="nucleotide sequence ID" value="XM_007372296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFF7"/>
    <dbReference type="FunCoup" id="G3AFF7">
        <property type="interactions" value="278"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFF7"/>
    <dbReference type="GeneID" id="18871165"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48009"/>
    <dbReference type="eggNOG" id="KOG0676">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062043_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFF7"/>
    <dbReference type="OMA" id="WDRQFGA"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="427" mass="48214" checksum="832CCDE900A85470" modified="2011-11-16" version="1">MAYTSPAVVIDNGSYTTKAGFAREDLPSLVFNSNYMVNPETQAVIVGDDALEANCQNEVMTLLDNGLIYNYDNIIHNWQYVYDNIDNHNPIDAKEYPLVLTEQSWNTPQNKVTTCQIVFESLEVPIFSLVKTPIAQLYRAGKSTGLVIDIGSSVTSVTPILDGIIQQKSSYHSKYAGDFLNLHVLNYLESKTPFNNLIPAQFAHASDSFKHYYASHNILQEYKNLSISYQIKSYQLYNHENINVQDQRNYLENLFDPTLNKLPNVTVPEPTLDKPGSHGLTNLVFLALKNLESSLLPSSNDTTSQNKFARFIEIFKELLSNVFITGGTASANGLPDHIINDLRALTQKYFPNYPFSYAVQQIRPNNTENSETWDRQFGAWLGACNLASMLNDHNEDSNSAKIALDNWFITKADYEELGEDLIVEKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFI1</accession>
    <name>G3AFI1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_58093</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372382.1">
        <property type="nucleotide sequence ID" value="XM_007372320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFI1"/>
    <dbReference type="STRING" id="619300.G3AFI1"/>
    <dbReference type="GeneID" id="18872318"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58093"/>
    <dbReference type="HOGENOM" id="CLU_1563849_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFI1"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="171" mass="19535" checksum="D1378594C674CCF5" modified="2011-11-16" version="1">MMKQDSMSRIEHLEQERDLLKQTLESEEKPEETEEITTDDVNTAINGFIMDLSRTPSVRETNRKKHEVDNSDKVSIELTPPVSDDEKEEESIQAQDVQNSNSTLDLVSAEISNTYSNDVEPFPKVHDQTHEEVVAVKKKFKLENEEFKQVRAIAIAISGVLIGYFIRRLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFN7</accession>
    <name>G3AFN7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">tRNA (uracil-O(2)-)-methyltransferase</fullName>
            <ecNumber evidence="3 11">2.1.1.211</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_69374</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Adenosyl-L-methionine (AdoMet)-dependent tRNA (uracil-O(2)-)-methyltransferase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>uridine(44) in tRNA(Ser) + S-adenosyl-L-methionine = 2'-O-methyluridine(44) in tRNA(Ser) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10339"/>
            <dbReference type="Rhea" id="RHEA-COMP:10340"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74478"/>
            <dbReference type="EC" id="2.1.1.211"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TRM44 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.211" evidence="3 11"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372438.1">
        <property type="nucleotide sequence ID" value="XM_007372376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFN7"/>
    <dbReference type="FunCoup" id="G3AFN7">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFN7"/>
    <dbReference type="GeneID" id="18875266"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_69374"/>
    <dbReference type="eggNOG" id="KOG3790">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018580_2_0_1"/>
    <dbReference type="InParanoid" id="G3AFN7"/>
    <dbReference type="OMA" id="IREPNIN"/>
    <dbReference type="OrthoDB" id="10047021at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141101">
        <property type="term" value="F:tRNA(Ser) (uridine(44)-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011671">
        <property type="entry name" value="tRNA_uracil_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21210">
        <property type="entry name" value="TRNA (URACIL-O(2)-)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21210:SF0">
        <property type="entry name" value="TRNA (URACIL-O(2)-)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07757">
        <property type="entry name" value="AdoMet_MTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 11" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW35026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="544" mass="62093" checksum="1833CD00A1A60D18" modified="2011-11-16" version="1">MTEELLTSQTSILGDPWIPIYRKSVDFGANHFESAMSNIIKEPNINSTVIMRADILKENIYDPKHGDTMFVSKLIHQTPEFPGSENTQDEVLHRDLHDVEIREIPNNGGLQLTKKCELVRRIIPRNPFKDHIINQTCLVLTGEDAVLVVYIPHIATKEEIPFYLPPVYGIGILYHKGQVSIHYLPFDEPGSTTEHDLLKSMPAAERPIRIAYRLLETSSKHSGGVKSGYEKRVNHDLVVPKIEFQNRYISLKKKYSSKLVNEWCESTDPKKHVFEDLAIAAFLIEYWILKKYNKDEFEFRDLGCGNGLLVYILNMEGYRGKGIDARARKSWAIYPEEVQQNLLEQIIVPSVLLKPHPAVSKMDPNIQDNGRRFKQIDKSESGVNVSFHTSESLLHSPKVCTTDEFPPNTFIIGNHSDELTCWIPLLGFPFIVIPCCSHALNGAKMRYPPRKAPTNTPQNSTYASLVDHVEDLSILMGWKVEKEMLRIPSTRNGAIMSNERIQPFQQEPEEIKQMRVLDIIAMEGGAEGWVENSMKLMTKEPRNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFY3</accession>
    <name>G3AFY3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58307</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372534.1">
        <property type="nucleotide sequence ID" value="XM_007372472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFY3"/>
    <dbReference type="FunCoup" id="G3AFY3">
        <property type="interactions" value="1168"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFY3"/>
    <dbReference type="GeneID" id="18872394"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58307"/>
    <dbReference type="eggNOG" id="KOG1769">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148322_4_2_1"/>
    <dbReference type="InParanoid" id="G3AFY3"/>
    <dbReference type="OMA" id="MKIYCAR"/>
    <dbReference type="OrthoDB" id="442921at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005940">
        <property type="term" value="C:septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16116">
        <property type="entry name" value="Ubl_Smt3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000155">
        <property type="entry name" value="Ubiquitin-like protein SMT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022617">
        <property type="entry name" value="Rad60/SUMO-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10562">
        <property type="entry name" value="SMALL UBIQUITIN-RELATED MODIFIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11976">
        <property type="entry name" value="Rad60-SLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="16"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="94" mass="10723" checksum="C84C0AF36EEDC63E" modified="2011-11-16" version="1">MSDTEQPKEEKQDSTTHINLKVSDGSAEIFFKIKRSTPMKRLMEAFCKRQGKDINSLRFLIDGTRVYPHNTPDELELEDGDTIEAHREQTGGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG10</accession>
    <name>G3AG10_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58335</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372561.1">
        <property type="nucleotide sequence ID" value="XM_007372499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG10"/>
    <dbReference type="FunCoup" id="G3AG10">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG10"/>
    <dbReference type="GeneID" id="18872410"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58335"/>
    <dbReference type="eggNOG" id="ENOG502RYTK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_927848_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG10"/>
    <dbReference type="OMA" id="NAGTWED"/>
    <dbReference type="OrthoDB" id="445556at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="63"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="321" mass="38128" checksum="243369325FF75D93" modified="2011-11-16" version="1">MFAVTVRNTLCRSNYAVQSASLLFYRHAHAHPTPQQDPHHHKSHINHHRRLDLHDWPTSKDPTPFEIFNLKQKDMGMPQLELKRHIKRHYLAYVKLYHPDTSLTIHEKGEELSAEEKRRRFDQIQQAYDLLKDPRRRVAYSRYQTARTRGPTYGPAQHGYTKEHFEAYRAANAQRRQYDFQNNEEFWQAGTWEDYYRMKYKREPPTKEEFEKNKYKILAGVIAFGALGFALQIVNTYNRTNEMLLDSQKRTLKSMHDLNEHSDNFGQGNSEADGLRRFLVSRRTSLKSKKYDEDTEPEPNDKELLVKYAKKRVGQWDKAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGT7</accession>
    <name>G3AGT7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein GLC8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_130711</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372832.1">
        <property type="nucleotide sequence ID" value="XM_007372770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGT7"/>
    <dbReference type="FunCoup" id="G3AGT7">
        <property type="interactions" value="288"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGT7"/>
    <dbReference type="GeneID" id="18869519"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_130711"/>
    <dbReference type="eggNOG" id="ENOG502S2VH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070379_1_0_1"/>
    <dbReference type="InParanoid" id="G3AGT7"/>
    <dbReference type="OMA" id="QQFQDIH"/>
    <dbReference type="OrthoDB" id="551302at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007062">
        <property type="entry name" value="PPI-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398:SF20">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY INHIBITOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398">
        <property type="entry name" value="PROTEIN PHOSPHATASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04979">
        <property type="entry name" value="IPP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="25981" checksum="88DBE109C58FF67E" modified="2011-11-16" version="1">MSDNKPRGILRNRDEGQHVPHEISDQLDRQEVIKNTQLNARLSSEYSTKGDKIRAKIAEEKRRRSFGASDHLKWDEINLYKTEQEKAATMKIDEPKTPYEGGFNPLGEYYRDDDNEEAAPEDDIPSFTLGEGEFDKEHPVVGSLSGGEVIPNEEAVVENEQPEDEENEQELTAEERHRRFEEKRKAHYHLKGMVLKQKFAVSDEDLDDEQEEKEPPQEEEQDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH08</accession>
    <name>G3AH08_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ABC1 atypical kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_64563</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372850.1">
        <property type="nucleotide sequence ID" value="XM_007372788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH08"/>
    <dbReference type="FunCoup" id="G3AH08">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AH08"/>
    <dbReference type="GeneID" id="18874982"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64563"/>
    <dbReference type="eggNOG" id="KOG1236">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006533_6_0_1"/>
    <dbReference type="InParanoid" id="G3AH08"/>
    <dbReference type="OMA" id="SMVRTHH"/>
    <dbReference type="OrthoDB" id="1290869at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044289">
        <property type="term" value="C:mitochondrial inner-outer membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055091">
        <property type="term" value="P:phospholipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13971">
        <property type="entry name" value="ADCK2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044095">
        <property type="entry name" value="ADCK2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052402">
        <property type="entry name" value="ADCK_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45890:SF1">
        <property type="entry name" value="AARF DOMAIN CONTAINING KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45890">
        <property type="entry name" value="AARF DOMAIN CONTAINING KINASE 2 (PREDICTED)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="2">
        <location>
            <begin position="205"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="2">
        <location>
            <begin position="293"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="688" mass="79246" checksum="353F026E98B19FEF" modified="2011-11-16" version="1">MIPRVFKPNILFKARCAVRFSTRTYYAPKPRLNHRVLGAATGVVSASILLYYNSQRISNDVHALDTSIHELPVQEVTRGETYEMSLYKASQREETEQYEEFRIQKKSHPNIIKRIYYYIRFTLTDYVIDPTITFIRFMELSLIFVPLLITSPICWIGKRNSGAKLWYKMLRHAAELAGASFIKLGQWAASRTDIFSQEMCHELGQLHSNVKPHSLRATKKIISKSFGDVPFDEIFDEFNEEPIGVGAIAQVYIGKLSQKTLERIDDEESHEGKDRWHLKLFQKILSSELHEPLSSSQWVAIKVLHPNVEVKISRDLKIMKFFAHLVNVLPTMEWLSLPDEVEQFSILMRLQLDLRIEALNLARFRSNFKDRLDIHFPKAFLQFTTRDVLVESFIVGVPMSKMLSLKDNFGKNLSKEVSDKGLDAFLKMLILDNFVHADLHPGNMMVRFYKNDLFQSEKEYKIVKSSQEVDNDKIVHELLALGNDEAAWCRKLAELYRDGYHAEVCFLDVGLVTELNEVDRVNFLDLFKALSEFDGYHAGELMVERSRTPETVIDKEMFAFKVEKLVDRMKSRTFTLGNISIGDLLDQVIGMVRKHHVRMEGDFITVVVAILLLEGIGRQLDPQLDLFASSLPVLRKLGVSKEGREILTNENALSMIKVWLALEVRQFINASIRDIHVLVKADMLSPNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH82</accession>
    <name>G3AH82_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_69733</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372924.1">
        <property type="nucleotide sequence ID" value="XM_007372862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH82"/>
    <dbReference type="FunCoup" id="G3AH82">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AH82"/>
    <dbReference type="GeneID" id="18875286"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_69733"/>
    <dbReference type="eggNOG" id="KOG2273">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014456_2_0_1"/>
    <dbReference type="InParanoid" id="G3AH82"/>
    <dbReference type="OMA" id="PPKNYIN"/>
    <dbReference type="OrthoDB" id="289314at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051079">
        <property type="entry name" value="Sorting_Nexin_Autophagy"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46979">
        <property type="entry name" value="SORTING NEXIN-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46979:SF2">
        <property type="entry name" value="SORTING NEXIN-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="10">
        <location>
            <begin position="119"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="209"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="347"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="541"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="349"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="371"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW35512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="672" mass="75378" checksum="DB74EE38D76219C6" modified="2011-11-16" version="1">MEPVNDSQQLNQNNTPQQELAAADQPLSDDEDNNPFIHQGLGSLVSQTSNGAKTNDKPGEVADPDEDDESMLLYNSNMATGTSATASTTKKPHETFNTVQMNFESRVTKLLKPNNKVKAQITEAGNSNEGMSNASKKYVVYTIKLINVEIPNDEILTRRRYSDFESLRDVLTKIFPLVVIPPIPPKNYFNFSVWNGLVGTSTNGIASTNGTSATDSSSSASAGGSAPVTTTTSGGKSLPTYSYINSTHLTKNKLIEHRKRLLTNFLNNCLEIRQIRHLEFFAKFLDPNANWTDEIALITSKLPKSIYLSNPENGLKTDPAYSHLPSPSSKNTISFFKDNGKRLTKKTNKLLSNDESASRQQSSPERDEVQNQDSASSPQTFNSTQYIVSTSTLDDINKRIMGNFIGLSNDYTELGTIFNSFSVVLGDFPGKSKSDDVKLNIIFDKIGQSFDRSYITINSLIGELETKFSEPLGEVVQYTSILQDIAKYKSRKFRQKQMLDTDIIEKKKDLTELLRLEEESSRIETAINSQGISKNTKFNLESATSRKSGQSPLKPTSNPQAQSTTTSKFRFPSFKKITQYVSEIIDQNPQQTRKQKIVQLQNKIKILERCQDIMLADLSFIADEVNKNIAAFHTRQLKMIYEILLCYNRFLIGWAKKNIDIWEEVKEEIIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHD6</accession>
    <name>G3AHD6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DNA polymerase epsilon catalytic subunit</fullName>
            <ecNumber evidence="17">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_133511</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">DNA polymerase II participates in chromosomal DNA replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="17"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373684.1">
        <property type="nucleotide sequence ID" value="XM_007373622.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AHD6">
        <property type="interactions" value="731"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHD6"/>
    <dbReference type="GeneID" id="18869755"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133511"/>
    <dbReference type="eggNOG" id="KOG1798">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000556_0_1_1"/>
    <dbReference type="InParanoid" id="G3AHD6"/>
    <dbReference type="OMA" id="MLDQCRY"/>
    <dbReference type="OrthoDB" id="10060449at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032183">
        <property type="term" value="F:SUMO binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035822">
        <property type="term" value="P:gene conversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05779">
        <property type="entry name" value="DNA_polB_epsilon_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05535">
        <property type="entry name" value="POLBc_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000002">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000010">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1600.10:FF:000006">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013697">
        <property type="entry name" value="DNA_pol_e_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029703">
        <property type="entry name" value="POL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055191">
        <property type="entry name" value="POL2_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054475">
        <property type="entry name" value="Znf-DPOE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670:SF0">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08490">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22634">
        <property type="entry name" value="POL2_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22912">
        <property type="entry name" value="zf-DPOE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23250">
        <property type="entry name" value="zf_DPOE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01159">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 17" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 17" id="KW-0235">DNA replication</keyword>
    <keyword evidence="14 17" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 17 19" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="12 17" id="KW-0408">Iron</keyword>
    <keyword evidence="13 17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 17" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 17" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase epsilon catalytic subunit A C-terminal" evidence="18">
        <location>
            <begin position="1515"/>
            <end position="1899"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU365029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW34100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="2208" mass="254513" checksum="1F3A34E56290B13C" modified="2011-11-16" version="1">MSKSNFKGSTSARFVKNDKITKYKTERPRQEIPVDPYVANNPMLNDAHQKRELVERIDKLDSIMGFDRFEHGEHDGAKPRKGWLVNMHATTIPSDQYLTGYSGVDYYFLDEEGGSFKATLQYDPYFFVEVLPGHESEVEEWAKKFLESCNVKSLTRVSKEDLALPNHLLGLKKNLIKLTFHNVSDLLGARRLLSPIIKENQMKRDSREIYKVMNFSNGHTNEESTNTTDPSTFLDDIKEYDVPYHVRVSIDKQIRVGKWYDVFAKHSKVHLVEDKEKIAFADPVVLAFDIETTKAPLKFPDAKIDQIMMISYMIDGEGFLITNREIISEDIEDFEYTPKPEYPGLFTIFNEPDEKHVLLRFYEHIRDAKPTVIATFNGDFFDWPFVETRSRFHDLDMFDEIGFAKDNEGEYKSKYCVHMDCFRWVKRDSYLPQGSQGLKAVTTVKLGYNPTELDPELMTPYAYEKPQLLSEYSVSDAVATYYLYYKYVHPFIFSLCTIIPLNPDEVLRKGTGTLCEMLLSVQAYENNILLPNKHSDPIERFYDGHLLESETYVGGHVESLEAGVFRSDIATDFKIDTAAIDELLRDLHNSIKFCVEVENGKKLEDVENFDQVYKEIESKLLELKSNPSRKETPLIYHVDVASMYPNIMTSNRLQPDSMKSEEDCAACDFNRPGKNCDRRLPWSWRGEYFPAEMNEYNMIKRTLQSETFPNSRSWLPPRTFDELSYTEQASLIKKRISDYSRKVYHRVKQSKVVTREAIICQRENPFYVDTVRSFRDRRYEFKGLAKVWKGKASKLDGADKDEAKKMVVLYDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGVTCLTGATIIQMARALVERIGRPLELDTDGIWCILPKSFPENFNLKCKDGKKIFLEYPCSMLNYLVHQRFTNDQYQDLVDPETFKYSTRSDNSIFFEVDGPYKAMILPTSKEEGKGLKKRYAVFNHDGSLAELKGFELKRRGELQLIKNFQSDIFKLFLEGNTLESCYQAVSTVANNWLDVLDTKGGMLEDEDLIELICENRSMSKSLAEYGDQKSTSITTAKRLGEFLGEEMVKDAGLATKYIISAKPIGAPVTERAIPVSIFSSDKKAIFLKKWLKDPSLSNFSPRDVIDWDYYRERLASVIQKIITIPAALQNVKNPVPRVQHPDWLKRKVTIREDSKQQSSIATFFGKSSKSEIMEKQVKDIEDFGEADYSMKSKVGKVTSKKRRAGRANLVADTEEEERNLAILNGPCPSMTEDYQGFLAYQKAKWTMQESNRERRRKLFGSNAESSQRSTVGSMIRKQAENVAGSNWEILQYKEDPTKPGNLKVYVSAGNKLHSFTFCIPKKVYASFKTELSERRLNVAGCDVEKSNAILPNGHDATNLYKFTMPQSTYQEQMTDVDSVLQDTNILGLYETQVGPVERAIIDLGNVVKFDDTRVGALGKGLKNGFNVKDLVKVDTDAYLRRFEMDMVYLLHIVTNSYEFFTVFKTWEPQAQIFVLKPSASAQELPANIDRIYREIYEAKKDKLGKVNNIIDYPSEMSFETVYYHDNAKLFKKLNQVLGKINESRSNTALLAIQSPYASRLLNVLTSTVDFPTIRMNISELALPALGWQALISKRVINHYFVLASWVKNLISLSKYGGVPLCNLQIENVGYLIDIAYARRLVQGNIVLWWSSKPLADHGGFEFDKVTDYENLEFPTINNPEIYETACLEVEIGTLEINTILTSSLINEAEGTDLADDAVRFDNNNGASSFAEDSFSAPALAILRSMVKDWWDDALKNNINADSMMNNLVTWVQRKDSFLYDYDLRYHVHNLTSKALLQLIGEFKRMNAHVIFANRNKMLIQTSKVSVENSYAYGQYILKAARSKPLFNFLDLKIVKYWDLLIWMDEFNYGGRCCTEITNEEMQNLVPENQWQLKKYLPVIFQNEFEDWLVIFLDALSKHKIETLTGGGTQTGTPRVTQIMHILKGQKNLEAKETEEEDITNGVIELFRRPLEKRMEKLYRRQNEAILNPEFKKEYEFPKLVGSHLHLRNPALELVKFLCAIFALSQKRNIEVRLLRKELLQLFDIKEFSKEASFRNPSASLKLPHVICDYCNYVRDIDLCREEEINIWHCVQCHKPYNKIAIEEEIIAQFNKTLTKFYNQDLKCSKCNQIRAGNLDLFCKCSGNWTETVSHHDVEKKLMVFENVASVFNLQLLKGLIEELY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHH2</accession>
    <name>G3AHH2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF202 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_135108</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373721.1">
        <property type="nucleotide sequence ID" value="XM_007373659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHH2"/>
    <dbReference type="GeneID" id="18869869"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135108"/>
    <dbReference type="eggNOG" id="ENOG502SF8V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113391_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHH2"/>
    <dbReference type="OMA" id="TFIRFAT"/>
    <dbReference type="OrthoDB" id="10261904at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052053">
        <property type="entry name" value="IM_YidH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187:SF2">
        <property type="entry name" value="DUF202 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187">
        <property type="entry name" value="FGR18P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="DUF202" evidence="7">
        <location>
            <begin position="38"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="153" mass="17053" checksum="AB9BBD4289F3025A" modified="2011-11-16" version="1">MSTISRQVTRESLSPSPSIVQYKFPYNLIMKVSTSEPRDGLQSERTCLTFIRFATALFFTSLVILLNFRLDTPHASPRSGLSTVLAYLLLGLSLVVLVISGVNYFVTIKRYAKGKIDNFGFNNFWSMVAVTGVIVALLAINVTLLIEEYLIHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHI6</accession>
    <name>G3AHI6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_59564</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373734.1">
        <property type="nucleotide sequence ID" value="XM_007373672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHI6"/>
    <dbReference type="FunCoup" id="G3AHI6">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="GeneID" id="18872974"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59564"/>
    <dbReference type="eggNOG" id="ENOG502S5B3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035821_1_0_1"/>
    <dbReference type="InParanoid" id="G3AHI6"/>
    <dbReference type="OMA" id="RIYVNCS"/>
    <dbReference type="OrthoDB" id="2556122at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044695">
        <property type="term" value="C:Dsc E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045226">
        <property type="entry name" value="Dsc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025390">
        <property type="entry name" value="Dsc3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019413">
        <property type="entry name" value="Dsc3_ub-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28049:SF1">
        <property type="entry name" value="DSC E3 UBIQUITIN LIGASE COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28049">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN YOR223W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13373">
        <property type="entry name" value="Dsc3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10302">
        <property type="entry name" value="Dsc3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="DSC E3 ubiquitin ligase complex subunit 3 ubiquitin-like" evidence="3">
        <location>
            <begin position="4"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="DSC E3 ubiquitin ligase complex subunit 3 C-terminal" evidence="4">
        <location>
            <begin position="138"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="293" mass="33772" checksum="B2D59EF1C46BAF30" modified="2011-11-16" version="1">MKFTLVIRFTNSESTSDQVHDLSIPLGINFERDDVNKLVTTNWIKSTIRSKVPQCRENRLRLIYNGRVLNENTDFKKEVFISRLHQEQASTEAPTQEDEKKIYIHCVIGEKLTQEQLAEENQLDNRPQEVTTTPQTISFDRLLQQGFSQEDVDDLRRQFYSIYGGSSATRRGGDDQITDVEEEEQRQRRLRELEERWIESTYNTEATDAPRPQSRRTETQDTDAAPPQPSLDLEESRVNEDILVGFLLGVFLGVIGAVFFLVDDTVFNKRQRMSLIAGLLVNCSLAIARGKWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHK8</accession>
    <name>G3AHK8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Putative sugar transporter, high affinity</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">XUT1</name>
        <name evidence="11" type="ORF">SPAPADRAFT_59608</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373756.1">
        <property type="nucleotide sequence ID" value="XM_007373694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHK8"/>
    <dbReference type="STRING" id="619300.G3AHK8"/>
    <dbReference type="GeneID" id="18872983"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59608"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_12_1"/>
    <dbReference type="InParanoid" id="G3AHK8"/>
    <dbReference type="OMA" id="AIMGACH"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="MFS quinate transporter QutD"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
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        <property type="entry name" value="MFS_dom"/>
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        <property type="entry name" value="MFS_sugar_transport-like"/>
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        <property type="entry name" value="MFS_Sugar_Transporters"/>
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        <property type="entry name" value="MFS_trans_sf"/>
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        <property type="entry name" value="Sugar/inositol_transpt"/>
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        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
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        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="84"/>
            <end position="104"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="111"/>
            <end position="133"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="139"/>
            <end position="159"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="171"/>
            <end position="190"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="210"/>
            <end position="230"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="317"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="355"/>
            <end position="376"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="383"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="418"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="455"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="485"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="39"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="553"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="553"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="RuleBase" id="RU003346"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
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            <dbReference type="EMBL" id="EGW34172.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
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    </evidence>
    <sequence length="576" mass="62296" checksum="2879159B1DE95005" modified="2011-11-16" version="1">MHGGSDGNDVQAIIAQKRLEIAGKPGIAGLIANRKSFLIAVFASLGGLVYGYNQGMFGQISGMTSFSAAAGVGKIQDNPTLQGLLTSILELGAWVGVLMNGYVADRVGRRWSVMFGVAWFILGVIIQACTHGANYSFILGGRFIVGVGVGILSMIVPLYNAEVAPPEIRGSLVALQQLAITFGIMISYWITYGTNFIGGTGEGQSKAAWLVPICIQMVPALILGSCIFLMPESPRWLMNEGNEEKCLDVLSRLRGLDRNNELIQMEFLEMKAQKIFEHELEATAYPDLQDGSASSRFKIGFLQYKSMLTHYPTFKRVAVACLIMTFQQWTGVNFILYYAPFIFASLGLSGKTTSLLASGVVGIVMFLATIPAVLWVDQLGRKPVLISGALLMGMCHFVVAGILGGLHGDFTNNMGAGWAAVVFIWLFAIFFGYSWGPCAWVIVAEVFPLGLRAKGVSIGASSNWLNNFAVAMSTPDFVAKATYGAYIFLGLMCVFGAAYVFFFCPETKGRTLDEIDELFGDTSGVSKREGEIRNRILKEVGLLELIGLEELDSDKSKGGDVHYQEEKAADADADSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHU4</accession>
    <name>G3AHU4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA mismatch repair proteins mutS family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_148870</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta, which binds to DNA mismatches thereby initiating DNA repair. MSH3 provides substrate-binding and substrate specificity to the complex. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. Acts mainly to repair insertion-deletion loops (IDLs) from 2 to 13 nucleotides in size, but can also repair base-base and single insertion-deletion mismatches that occur during replication. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer consisting of MSH2-MSH3 (MutS beta). Forms a ternary complex with MutL alpha (MLH1-PMS1).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007373780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHU4"/>
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        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHU4"/>
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    <dbReference type="KEGG" id="spaa:SPAPADRAFT_148870"/>
    <dbReference type="eggNOG" id="ENOG502QUUG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002472_4_0_1"/>
    <dbReference type="InParanoid" id="G3AHU4"/>
    <dbReference type="OMA" id="DTWIMRR"/>
    <dbReference type="OrthoDB" id="2534523at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032139">
        <property type="term" value="F:dinucleotide insertion or deletion binding"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032137">
        <property type="term" value="F:guanine/thymine mispair binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043504">
        <property type="term" value="P:mitochondrial DNA repair"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF34">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="DNA repair protein MutS, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="9">
        <location>
            <begin position="831"/>
            <end position="847"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="950" mass="107908" checksum="8B656C53CF4C0DEC" modified="2011-11-16" version="1">MQTRQLARRVILSPSIQIKRMLSITQYVRHEIATATTATAATATSVVSDVHEKQVATDSPSIGTIPGWDRGGRKQDKSLTALYATIRRLIDKNPGSASLVQVGSFYEMYFEQAEQYAPKLGLKLAIKRTKNYAIPMAGFPLSQLRKYVEILVHEIGVNVAIIEQYPTSDAVQLNNLIHRKISRIVSPGTLIDESFLNYNSNNYLLAISFPPHLTDLPPDPQTPVGLAWVDISVGESFIQQTTLKDLSADISRISPSEILLTQDCQPLELHTGKWESSLQILRKYFLRYHSAQHSDLKLQFKSSLQATRRALQNLSVRDHSALNMILSYVRINLPEANFSLELPTYYYNKNVLQMDPRTREALELTERSVNGRASVIGSFLTTIRETATASGSRLLTQWIKSPILDIRELQRRQGYVKLFLDYPMLRRTMRMHLSQIGDFIRFVQRLTVGTGDDVVQLVSIADSLSKLQKLEEYLKEEYAKQKDMTVLGDFLKKFHVPTGIADEIHKTIYIKPVIKVEDSEEKVDESDESALEMDMDKYRIKQTDELSNEPDFSFSVNRDFNETLSKLHSDLDNLERKEQVQIANVQQILTEIDPKLTVSKKEQYGRYSDVLYISGKQKSIVTAFEKFDQDVRERKQKSFIYKPKEWDDLQRKIHEIHNAIVSEERKIIEYLKVKVIDEIPAIRHVSRLVDFLDVTSSFSILAQENHWTCPELTDSPCLEIKQGRHVVVESSLREGGGNFTPNNTNLGTDGKLWVISGPNMGGKSTFLRQNALIVILAQIGSYVPCEYAKVGIVDKIFTRIGASDDLFNDLSTFMVEMVETSNILRNASSRSLAIVDEIGRGTSGKEGMAIAYATLRNLVEVNQCRTLFATHFGKELQSILQSNNVDQKDIRYYRTRVLVNESDSEFEKKWIIDHHLEPGISETSNAIEVAQMAGFPERALDVARDVLLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHY6</accession>
    <name>G3AHY6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_49353</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373884.1">
        <property type="nucleotide sequence ID" value="XM_007373822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHY6"/>
    <dbReference type="FunCoup" id="G3AHY6">
        <property type="interactions" value="757"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHY6"/>
    <dbReference type="GeneID" id="18871328"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49353"/>
    <dbReference type="eggNOG" id="KOG0018">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001042_0_1_1"/>
    <dbReference type="InParanoid" id="G3AHY6"/>
    <dbReference type="OMA" id="KHMDFQR"/>
    <dbReference type="OrthoDB" id="5575062at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030892">
        <property type="term" value="C:mitotic cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003680">
        <property type="term" value="F:minor groove of adenine-thymine-rich DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03275">
        <property type="entry name" value="ABC_SMC1_euk"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028468">
        <property type="entry name" value="Smc1_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937:SF12">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="12">
        <location>
            <begin position="523"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="205"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="325"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="394"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="683"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="826"/>
            <end position="930"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1231" mass="140809" checksum="FCDD4B78B9D2B406" modified="2011-11-16" version="1">MGRLIGLELYNFKSYKGKTSIGFGTSFFTSIIGPNGSGKSNMMDAISFVLGVKSSQLRSQNLSDLIYRGRKENVDEDTTINSTDQDPTTAYVMAVYEKDSGEILKLKRAITVSGTSDYRINGRSVTLLNYSKVLKQENILIKARNFLVFQGDIEQLASQSPKDLTSTIEQISGSDEYIQEFEKLKEESERAREFSSSVFTRKRTLNSESKQYKEQLAEQREFEEKLVLKGDAIKKIHLYKLYHNEKKHDLLKQEIKDKKNELKERKRDLQTKEKTYETIMSEYSSVALDLKRIKQKIDTSDMKVESTKRDLIPIEATKRALASKISSRRTKIKDLEKDIANQKQQVSVIESQLRDAERLSREFEQSNVDSDISISPEAQREYSELRSKFLATDESRLEQDLTLLSNEKEKINANIVNIQSQKEHAEATVREIESLVVSELKSKYDDINTEINNTLELKAQKEELRKSLIKKKNDLESKRKQLDIQYFDVLSKINESRAQQQESSKQKQLRENVSMLQQSFPTGAVKGLVYELVRPSASKYEQALATMLGRNFDAIIVQTSAVAHKAIEMLKERRAGVATFIPLDTIDVDPVNLHHLRSLATPGLDIMEYEDKSLENAMNYIAGDALIVENMTVARQLKWTGGSRVQNKLITLQGSVIHKSGFMTGGGSSQKSTVVLNWDKRELSRLNEVKDDLDIQIRKVEDEMPTTAEISSLTDEISQFDNKLVVLRDERDNKERVIKDRESEIEFQKSLSLGFIDAIAQKRNELKKLDVKMKHISDEIKLAKEDIFGEFCEKYGFVNGIEDYEKTHGNTLRTRAKERVQYSKAISTLANKLEFEQERCKKTQERKASLESKLVDLEHEFDEVLKAKTELEETLDSTEAENEVMKQELITITNSTQAKLKTAKLIESDVQEAQAEFDSTNRELLQIEERLLKVDSERATALVNCKIENINLPLNDGDLESIPVGEKSQEAIDEIYKLEIDYSLLDERFKEAYNIHLESELAASLQTILDQIEQLTPNAKAQERLQEVQAKLTEQDRDFTVARQRERQINKKFEEIKEERHKKFMDAFNHISSQIDGIYKELTKSAVAPMGGSAYLTLEDSEMPYLSGIKYHAMPPMKRFQDIENLSGGEKSMAALALLFAIHSYQPSPFFVLDEIDASLDYANVMKIGNYIRKNAGPNFQFIVISLKNSLFERSDSLVGIYREQRENSSKTVSLDLRDYPEEEVALTASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI80</accession>
    <name>G3AI80_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_59825</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">The C-terminal domain binds 2 Fe-S clusters but is otherwise mostly in an intrinsically disordered conformation.</text>
    </comment>
    <comment type="domain">
        <text evidence="9">The N-terminal domain has structural similarity with S-adenosyl-L-methionine-dependent methyltransferases, but does not bind S-adenosyl-L-methionine. It is required for correct assembly of the 2 Fe-S clusters.</text>
    </comment>
    <comment type="domain">
        <text evidence="9">The twin Cx2C motifs are involved in the recognition by the mitochondrial MIA40-ERV1 disulfide relay system. The formation of 2 disulfide bonds in the Cx2C motifs through dithiol/disulfide exchange reactions effectively traps the protein in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the anamorsin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373978.1">
        <property type="nucleotide sequence ID" value="XM_007373916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI80"/>
    <dbReference type="FunCoup" id="G3AI80">
        <property type="interactions" value="174"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI80"/>
    <dbReference type="GeneID" id="18873091"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59825"/>
    <dbReference type="eggNOG" id="KOG4020">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067152_0_0_1"/>
    <dbReference type="InParanoid" id="G3AI80"/>
    <dbReference type="OMA" id="TMITCGK"/>
    <dbReference type="OrthoDB" id="311633at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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</entry>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
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                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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    <reference evidence="8 9" key="1">
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            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Sato T.K."/>
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        <property type="entry name" value="PH-like_dom_sf"/>
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        <property type="entry name" value="PH_domain"/>
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        <property type="entry name" value="SAM"/>
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        <property type="entry name" value="SAM/pointed_sf"/>
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    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22902:SF27">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY A MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SESQUIPEDALIAN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SAM_2"/>
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        <property type="entry name" value="SH3"/>
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    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="7">
        <location>
            <begin position="195"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="582"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
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            <begin position="264"/>
            <end position="288"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
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            <begin position="330"/>
            <end position="561"/>
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    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="758"/>
            <end position="848"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="4">
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            <begin position="83"/>
            <end position="94"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
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            <begin position="268"/>
            <end position="288"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="4">
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            <begin position="389"/>
            <end position="409"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="4">
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            <begin position="464"/>
            <end position="478"/>
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            <begin position="497"/>
            <end position="506"/>
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            <begin position="511"/>
            <end position="520"/>
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            <end position="537"/>
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            <begin position="785"/>
            <end position="800"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="869" mass="94592" checksum="0A763E452E9B2569" modified="2011-11-16" version="1">MTGAIYICIKQFNARLGDELSLKVGDKVEVIADDSEYNDGWYMGKNLLTNEVGLYPNSFTQLLVPEESEGELLRSRSRSRHVGASTATAAAAASGPPPTGLGIIGANTTPTIAEHDDHEPIMVDAFDKLNINAKMNANTSHNNLNSEIDKALKEIKDGHPSPDNTILSKESITFSTSTSNHDDVGILDPSQAISWSPKEVAVHFMRRGFDSDISNKFVQHKITGDILFQLELAHLKELDINSFGTRFEVNKEIERLKGLIKKPKLESRTNSQSTSSTHKDTNSFYSPPSAATTLITEERADEFAKPKPIIEQQQNQHQEQLMPSVMLSPSETTFKSHKKSKSQSLDDLTKPPTPAYTPRKAPEPPTSNGNSPMGSTFRFGAGSEDLRSPSGSYMTRTAASERPSSSLYDSGSAYHKRNDSVKRTTSPHKRHSSLFSFLSSDDVGKKKDDLANIPPLNVEDKRAATPATGGTAGTAGTDATDKSSSSPVDIDDASLSPKRKKVLPRIKTKDLFSNNGNNTDSSDEPKSDPTSLSNAMPSTSRLKSFRSSSTQNFRNLTTSKKSKTSAFQEGIREINPEEAIKTANFSGWMSKRSGNTLAWRSRFFTLHGTRLSYFTSLKDNKEKGLIDITAHKVLPISTDSDSDKYKALIASSTLAGSYCFKVVPPAPGFKKGLTFTQPKTHYFAVNTEEEMRGWMKALMTATIDIDDSVPVVSSCSTPTVSLGKAQELLAKAREETKLKDEELRAQGYIRVHDDFEDSVDSTSSSINGTTGTIKRQQKHSSDMNRSMTTPTTPRATSGTGFSSPYMLAGGVISSSKPHTPVHGGPQANSTPPLGNGPMMTTPGSGKKEKLLAYSSDMSGNHTFVIKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIT7</accession>
    <name>G3AIT7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_149476</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in a process influencing telomere capping.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Vacuole</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_007374025.1"/>
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    <dbReference type="eggNOG" id="KOG0309">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_001497_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIT7"/>
    <dbReference type="OMA" id="HRRETCL"/>
    <dbReference type="OrthoDB" id="311712at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:Seh1-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16488">
        <property type="entry name" value="mRING-H2-C3H3C2_Mio-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C1-like_sf"/>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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        <property type="entry name" value="WD40_repeat_CS"/>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
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        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049567">
        <property type="entry name" value="WDR59-like"/>
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        <property type="entry name" value="WDR59_RTC1-like_RING_Znf"/>
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        <property type="entry name" value="Znf_RING"/>
    </dbReference>
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        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
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        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
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    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <keyword evidence="4 7" id="KW-0853">WD repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="108"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="196"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="1371"/>
            <end position="1409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="461"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="823"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="898"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1112"/>
            <end position="1146"/>
        </location>
    </feature>
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        <location>
            <begin position="837"/>
            <end position="850"/>
        </location>
    </feature>
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        <location>
            <begin position="859"/>
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        </location>
    </feature>
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        <location>
            <begin position="907"/>
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    </feature>
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            <begin position="924"/>
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    </feature>
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        <location>
            <begin position="1112"/>
            <end position="1125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002738"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW34503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1421" mass="160969" checksum="90452290265D677F" modified="2011-11-16" version="1">MVVTNPHDSPTFGNSLSLKVDGAIGAMTLSPNGRDCVLAGRRGLFIIDLDDPFTPPRWLHHITSWEVADVQWSPHHTLKPSWCISTSNQKALLWDLQRPSDNAIANILHRHTRAISDINFHPFDCEMLATCSIDTFVYSWDMRTPRKPVAQWAEWRAGSSQVKWNHENPFEIASCHDHSFYIWDTRNGALPILKVDKAHQGKINGLDFNQGLKNVITCSNDNTVKFWDLQSGQAKEYISHYNYFERSKEELQPSVVIKMDFPIARARQLPFGKDMACGIMPLRGGDDSVHIVNYDTAYHDYLSTGTTQVIEGESVYSFKGHNGPMKDFLWRTQHEKYENYESKKKWKDYQLVTWSAVDYDLKLWPDSEELYKSVNYDPSFVNVFKGSDSDTSRPHTPLEEQTYNYDTYCVEPSVTIDDLTRTNNDLLSSLTLLRISEKHKQAGVEFTQLNHLDWISGVRMGRPAQSSDRKDSTGSFEDDGPLNLGEEVSIVGHKFPKIRFEKIAVSSGELVISLRGPIPDMSTTMSKADTSDMLFAENTSVAGESTAISNKDKRISVHSDDGHEQSISHKEESVKDNTISALTPIDETSEQKLIFIRLKVNFPKTYPYLEDVDYNKKRSKLSKSNLIRFHIEVTHELTKEIRDVMQEKLSEISQFYTNKYQRFCLEPCLRSLMGEKVELDDEIMMESGANNNNEELEQESVQEVGNEGWADDLIRQQPDFRGYSSGEDDVEYADLIPAVNDSLVLTQNLDSIMHPAQRAAVAARQMYYDSTPQPKGCGAVWSRSGQLVCFFIPKSQDDDQNDSKVLQKFNIFKFTDRGFSVKANQQSHHHHHKLEESISDSDLEGEDSDAEPGSRSDGESGSSSSEDSFTNDWDEILQDETSSRARVPRMFKTTLGLGNRYSESKKSSSINKLPSQGGTSNHRSSVKSKKKPKSQVKNRNIVGIFDFSHLLPDKYELACEYRVLGDVPENLAAYNSKVALKYGLKEISEVWKMLAILLVKNGQPLQMNNGYFPDQMNNSTKLADKFHSFYWGNHPFGSTWLIKEIFDYFEKRGNVQMLAMMSCILYENPKNLAMPDSPATDIAYNVPIHTPYQALPQPPSIAALSRLNRQNSNSLPQSILQPSSQDKFPLMQHPNSSHSSFKRDSSVSGVTYERSINSSLDGASQVGSPERFGYMKKNLLLTAPVAASEHSYSSLSDSVSEGLRATSPNQMTNKLKFDKRMISKMPVKRGTSLGAGPGLIKPKSTKIRPPPSVTIEMQNVEELDLFENVYSRPLLSSQDVEKIRTYRELYADMLYNWGLPVSRIKILKFNYPDIDEGELETSPFDSHKCKIGLRKRNKQLPQQNFLNAVSTIMQKKPNAWNTNKRSIMKYCNLCNLVVTKNFTMCIVCEHILHTDCAMEWWTGGASECPSGCGCTCLEHSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIV8</accession>
    <name>G3AIV8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA polymerase epsilon subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">DNA polymerase II subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_48886</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase epsilon subunit B family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373353.1">
        <property type="nucleotide sequence ID" value="XM_007373291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIV8"/>
    <dbReference type="FunCoup" id="G3AIV8">
        <property type="interactions" value="776"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIV8"/>
    <dbReference type="GeneID" id="18871279"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48886"/>
    <dbReference type="eggNOG" id="KOG3818">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010628_1_0_1"/>
    <dbReference type="InParanoid" id="G3AIV8"/>
    <dbReference type="OMA" id="PEDGAWF"/>
    <dbReference type="OrthoDB" id="10254730at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045005">
        <property type="term" value="P:DNA-templated DNA replication maintenance of fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016266">
        <property type="entry name" value="POLE2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12708:SF0">
        <property type="entry name" value="DNA POLYMERASE EPSILON SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12708">
        <property type="entry name" value="DNA POLYMERASE EPSILON SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="8">
        <location>
            <begin position="354"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="660" mass="74626" checksum="AFDB8DA794A411D7" modified="2011-11-16" version="1">MESTTSLPIKLQPSNLRPFAFRILSKKHGLNINTDALAVLTETISFKFGFDWKGPRTHQFLEDIAKIWKVEDRGLFIDGAGLKQIIKNMNQKSETSSFSDKATRSDTIVDVGEDALNWDDYFKFITPEDQPRSIFDKSRKQFNISTKKKGSLFQSLSENLSLSIDTFNNRYHLLSDRLSRNENFQKSSTVSISSLSMSLKDGNLANEITLIKNVLGRDGQKFILFGLLSKNANDQYILEDSTDNIELNLSQTHKTEGSFYCTGMFVIVEGIYSASGGSSNQAHDYIGGCFYVSTIGQPPAERREKSLDAYGNVDFLGIHKQAAVSGEKITKITKTLKRKLTTLERSLIDHKFIFVGSDCFLDSFKVLDGLKKFFQRLENSIIESIESDEKSTPLALVLTGSFSSQPLHTTSSSTTAISQSETYKSNFDSLTNLLGNYPNIVQTCKLVLIPGKNDPWQSGYSLGASSMNYFPQRSIPNAFVNRLEELLPRGNLILGWNPMRINYLSQEIVIIKDEYMNKCKRNDIVFTSDLESEQERTRRDSAIYEERIESIVQGGNRTEILSSKIKHARKLVKTLLDQGNLQPYTKDLKLINPQYAHALRIEPLPTVLILHDAGFDNFEVTYNGCKVVNIGQAVSENNRKLNYAEYHPSSKKFEFKDLHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIY0</accession>
    <name>G3AIY0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Sulfite efflux pump SSU1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_147854</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tellurite-resistance/dicarboxylate transporter (TDT) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373374.1">
        <property type="nucleotide sequence ID" value="XM_007373312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIY0"/>
    <dbReference type="FunCoup" id="G3AIY0">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIY0"/>
    <dbReference type="GeneID" id="18870766"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_147854"/>
    <dbReference type="eggNOG" id="ENOG502QT02">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030057_6_2_1"/>
    <dbReference type="InParanoid" id="G3AIY0"/>
    <dbReference type="OMA" id="FLATCPM"/>
    <dbReference type="OrthoDB" id="1099at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000319">
        <property type="term" value="F:sulfite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09318">
        <property type="entry name" value="TDT_SSU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.150:FF:000004">
        <property type="entry name" value="Malic acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.150">
        <property type="entry name" value="Voltage-dependent anion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004695">
        <property type="entry name" value="SLAC1/Mae1/Ssu1/TehA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051629">
        <property type="entry name" value="Sulfite_efflux_TDT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038665">
        <property type="entry name" value="Voltage-dep_anion_channel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31686">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31686:SF1">
        <property type="entry name" value="SULFITE EFFLUX PUMP SSU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03595">
        <property type="entry name" value="SLAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="188"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="296"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="331"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="421"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="21"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="461" mass="52003" checksum="9B3BC681D236FA41" modified="2011-11-16" version="1">MSDSNQIIVASTNDATTVATTDEHPHQENHSDRTDVDSNISDTTENITCDPNGKHMDREDSIYSGVSYSSKLSFTSRIYNLVIEDATKNFHPVYFVSIMGTGISSSILYNFPFEAEWLRVCSYIMFAFTCLFFLGTTTLFILSCKYFPGRFREYHVDTSKAVFMGCYSMGYITIVNYIEILTHDRHTIFVWVLWWIAVFSAMFTAFIIVFFSFMSKLNKVDTAAKLNATLLLPIVAITVVSSSGHQLELRLPTMNQTVITMLVSFMLWCLSISLAFIIITIYFWRLIVHKIPPTHLVFSSFLPVGFLGQSSYSILLMGINLDVLIPSDQPWGKILLCICGFFSMFLLSFGYFMTFVAISSIFSKIRPFAKNPNPKHTNQHGFLKIHKGFWAMTFPLGTMSLSNTEIGKGTIGNYPLITFRVMGAIFGTACILVTVSCIIGVIVTIVKKLNSLFIRRQISIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ78</accession>
    <name>G3AJ78_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_134376</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ78"/>
    <dbReference type="STRING" id="619300.G3AJ78"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134376"/>
    <dbReference type="eggNOG" id="ENOG502SADP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_195672_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ78"/>
    <dbReference type="OMA" id="HYAYGFY"/>
    <dbReference type="OrthoDB" id="2278877at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043457">
        <property type="term" value="P:regulation of cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044792">
        <property type="entry name" value="TAR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47188">
        <property type="entry name" value="PROTEIN TAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47188:SF1">
        <property type="entry name" value="PROTEIN TAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="81" mass="9157" checksum="954CD9112DFC7D43" modified="2011-11-16" version="1" fragment="single">SFAPIPKFDDRFARQNRYEPPPEFPSASPYSGIVHHLSGPNSYALTQIHPKTSGSVDDAPRRALTYVHFHYAYGFYTQTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ86</accession>
    <name>G3AJ86_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_59202</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373427.1">
        <property type="nucleotide sequence ID" value="XM_007373365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ86"/>
    <dbReference type="GeneID" id="18872818"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59202"/>
    <dbReference type="HOGENOM" id="CLU_3015683_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ86"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="56" mass="6070" checksum="507F6B63AEFB7925" modified="2011-11-16" version="1">MTASLKKTDSANVSIISTQSSISSIKSKTKSIIGKFKGKRKESTHLGTIVEYLAIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJD0</accession>
    <name>G3AJD0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ATP synthase f chain mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_60022</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374173.1">
        <property type="nucleotide sequence ID" value="XM_007374111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJD0"/>
    <dbReference type="FunCoup" id="G3AJD0">
        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJD0"/>
    <dbReference type="GeneID" id="18873183"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60022"/>
    <dbReference type="eggNOG" id="ENOG502S739">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152700_1_0_1"/>
    <dbReference type="InParanoid" id="G3AJD0"/>
    <dbReference type="OMA" id="HHKNNVH"/>
    <dbReference type="OrthoDB" id="5561579at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019727">
        <property type="entry name" value="ATP_synth_F0_fsu_mt_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28161">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT F, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28161:SF1">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT F, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10791">
        <property type="entry name" value="F1F0-ATPsyn_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="99" mass="11086" checksum="8EB203292EFF2181" modified="2011-11-16" version="1">MSFVIRRQLSTLIPPKIASARSLGSNPHAKRMADVVSFYQKLPKGAAPAPKPTANPFTKYYRAYFEGDNASGKPLLHLIALVVVVGYTWEYQSHLKHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJF9</accession>
    <name>G3AJF9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_148020</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373446.1">
        <property type="nucleotide sequence ID" value="XM_007373384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJF9"/>
    <dbReference type="FunCoup" id="G3AJF9">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="GeneID" id="18870770"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_148020"/>
    <dbReference type="eggNOG" id="ENOG502QT05">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036005_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJF9"/>
    <dbReference type="OMA" id="HITFRGA"/>
    <dbReference type="OrthoDB" id="10257314at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000907">
        <property type="term" value="F:sulfonate dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044273">
        <property type="term" value="P:sulfur compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000003">
        <property type="entry name" value="Alpha-ketoglutarate-dependent taurine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051323">
        <property type="entry name" value="AtsK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468:SF1">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="7">
        <location>
            <begin position="82"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="381" mass="43419" checksum="DCA94026B861D381" modified="2011-11-16" version="1">MAPVAVDKYDASQFQTAHNSKTKNGAFTVSEKNQHLSKYPEYLPTWNPSQKFPPYKFQKYYDQGLLADSKLSNLFPKDGDYQVKKLTPRFGSEVSGIQLSELTNEAKNDLARFVAERGVVAFRNQDFNSKGPEFAIKFAEYFGPLHIHPTSGSPEGFPQMHITFRGATQEEIETPFRSSTNSISWHSDCSYSLNGLQLTLFSCLQLPESGGDTLFANCVEAYKRLSPAMQERLEGLHVLHSSIEQAEANKRAGGITRREPEANIHPLIRQNPVTKEKSLYLNKEFGRKIVELKHEESDYLLNFLYDHVEKAHDLQVRIQWSENTVVLWNNATTVHTPCVDFSEPVIRHAYRISVMGERPVSDVKYLNDETYLSDKYELLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJG5</accession>
    <name>G3AJG5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">60S acidic ribosomal protein type P2-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_148037</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373451.1">
        <property type="nucleotide sequence ID" value="XM_007373389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJG5"/>
    <dbReference type="STRING" id="619300.G3AJG5"/>
    <dbReference type="GeneID" id="18870771"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_148037"/>
    <dbReference type="eggNOG" id="KOG3449">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114656_0_1_1"/>
    <dbReference type="InParanoid" id="G3AJG5"/>
    <dbReference type="OMA" id="THIVFPY"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002182">
        <property type="term" value="P:cytoplasmic translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05833">
        <property type="entry name" value="Ribosomal_P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044076">
        <property type="entry name" value="Ribosomal_P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141:SF103">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442363" evidence="5">
        <location>
            <begin position="17"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="62"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW33867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="108" mass="10951" checksum="E0152C3CAB7890BB" modified="2011-11-16" version="1">MKYLAAYLLLVNAGNATPSAQDIKNVLAAADIEVEEDKVEKLIAEVEGKNVEELIAQGNEKLSSVPTGAPAAAAGGASAGAATEAAEEAAPEEAAEESDDDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJH0</accession>
    <name>G3AJH0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_59248</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373457.1">
        <property type="nucleotide sequence ID" value="XM_007373395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJH0"/>
    <dbReference type="STRING" id="619300.G3AJH0"/>
    <dbReference type="GeneID" id="18872835"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59248"/>
    <dbReference type="eggNOG" id="KOG0712">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_10_5_1"/>
    <dbReference type="InParanoid" id="G3AJH0"/>
    <dbReference type="OMA" id="HTCEVNL"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000245">
        <property type="entry name" value="DnaJ domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044713">
        <property type="entry name" value="DNJA1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43888">
        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="6">
        <location>
            <begin position="6"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="7">
        <location>
            <begin position="199"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="5">
        <location>
            <begin position="199"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="522" mass="58021" checksum="FAC2F056F314C59E" modified="2011-11-16" version="1">MVKETHLYDILEVSTAASTEEISKNYKRRALKCHPDKTNHDPQLTEQFKEMTRAYEVLRDPNQRTIYDQYGEEGLSGVVVDEPQQAPAAAAAPPPVPPRRRSAFATDIFSQVFNDINSMFSQGPMFEFSATSAPGSGHPFMSTTTSTSTIFDGTQFSSSASGSFENMKKIVEPWGEQEFMRGEDIHHTCTVNLADLVYGKTIKLQLPKNSKCGVCQGNGGFNPKTCIQCRGTGKIKTTFCNRFSRFQQTGSCKPCNGTGIFTHYKDKCPNCKATGYIQQSKLIKVNVYPGSKDGDRIILPSEGDEGRNIIPGDLIIHLRQNRHPFLVRKGHDLFMDHEIDLKTALLGGEIQIPNFIKQGQLLKVYINVHGIESVNNNDSIQQGEIVGVINSDEPKILKGLGMPINEMVKGGEIIENLNEVEEMKEVLFDLRRYNRGNLYINFHVKLPNIGDFSQMDLLQLQSILPSAQPTGENGSEIIESNLANLPGNPRIYKSVSPTKSSSGDIDMDINGGSYVNGKRRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJM9</accession>
    <name>G3AJM9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59314</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373514.1">
        <property type="nucleotide sequence ID" value="XM_007373452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJM9"/>
    <dbReference type="GeneID" id="18872861"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59314"/>
    <dbReference type="HOGENOM" id="CLU_1860087_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJM9"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="138" mass="15972" checksum="5168CFA237B320E5" modified="2011-11-16" version="1" fragment="single">MGDMKYLSDSMTFQLSNKDNFEPNELTRPDIMLVEKPVLLTNPIPSNYSSIDKRTKARRIYPEKFSNNETEGEESDDDISTPMVQKMTKDDDTTNFTNNSHNPRIERIKHGINKLSVTPKKLTFDDRNITAQFINKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK55</accession>
    <name>G3AK55_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_49800</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374381.1">
        <property type="nucleotide sequence ID" value="XM_007374319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK55"/>
    <dbReference type="GeneID" id="18871373"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49800"/>
    <dbReference type="HOGENOM" id="CLU_567622_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK55"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="484"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="541" mass="61665" checksum="CF22B36C1D24B19E" modified="2011-11-16" version="1">MTSISLIFKTIWGLFTPAEEPVPIVESGSDADIQTTEIPSLRSKLRSFFYKSKRNSPNENPSDVSSIPSNFTPDLQETSTLKSVPSDHKHKHHFSKLSNWFRKRSRENSSDIATSNEQDSSYIPSLPQSSTDQNVTSSQFIQSQFGLVSEMSGNLKKTKALLAWKNLKTTERKILDKYCLECCQTPRRETTIKKLQEQLQVLEVRLFEAENQYGERIPDDIRNSMNEFAVSTRDICGKLVNNTDTFVASLNKESPTLSSKINSASAKSRQESISSTIFQMLNQEIPKFKADSENHNATPEIIETSSIYNSLTNLLTGKHCLHSQVASSHIHNVAPKCIQSSSVYGSLLTMRMSSCSQEANASGIIHSDIITYNGGLVPGSIVHESTRTNPEISFSNQSESTILLRAPWQYENFNWPHLKDGSASKFLDKRNKEVIVKIQRELEEDKRRILRNQERKEIKRMEHENSLREYEDYNSLDLYINQSDSEDSEKERIRSRPVTRSDTYSDLVHKSKQRREKFRKDIIQKCAGSALICPNTTRPLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKB1</accession>
    <name>G3AKB1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_151883</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRP18 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375085.1">
        <property type="nucleotide sequence ID" value="XM_007375023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKB1"/>
    <dbReference type="FunCoup" id="G3AKB1">
        <property type="interactions" value="544"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKB1"/>
    <dbReference type="GeneID" id="18870911"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_151883"/>
    <dbReference type="eggNOG" id="KOG2808">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081028_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKB1"/>
    <dbReference type="OMA" id="RRWIISI"/>
    <dbReference type="OrthoDB" id="10261918at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071021">
        <property type="term" value="C:U2-type post-spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000386">
        <property type="term" value="F:second spliceosomal transesterification activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.940.10">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004098">
        <property type="entry name" value="Prp18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039979">
        <property type="entry name" value="PRPF18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13007">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13007:SF19">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02840">
        <property type="entry name" value="Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47938">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Prp18" evidence="9">
        <location>
            <begin position="137"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="277" mass="32133" checksum="A5AEBB9392EA3F0D" modified="2011-11-16" version="1">MDFSSLISKEINKKRKKVGKTNAEKPELKSSLDEKQFTTISEPAEDTTKTTEEPPLTEEQLDYKLSQFDQPTDNLTTKEKITKLKQLLRYQSRNEKYKEWLDKEAPYYQDPTKQQVTLDLISNLQSRKDKVNVLARVYIKEMIKSWQDCNEAHPSDQQTALLFETKRDIVPLLYGLRTGELSEDILTSLTTIIYYIQQKDYRRANESYMKLSIGNVAWPIGVLNVGIHARSAAERITGQKKQANIMIDDKTRRWITSIKRLITAKERIDNGSGHVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKE2</accession>
    <name>G3AKE2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Repressor of RNA polymerase III transcription MAF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_150258</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mediator of diverse signals that repress RNA polymerase III transcription. Inhibits the de novo assembly of TFIIIB onto DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374414.1">
        <property type="nucleotide sequence ID" value="XM_007374352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKE2"/>
    <dbReference type="FunCoup" id="G3AKE2">
        <property type="interactions" value="107"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKE2"/>
    <dbReference type="GeneID" id="18870860"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_150258"/>
    <dbReference type="eggNOG" id="KOG3104">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037043_2_1_1"/>
    <dbReference type="InParanoid" id="G3AKE2"/>
    <dbReference type="OMA" id="INIGPFG"/>
    <dbReference type="OrthoDB" id="277029at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000994">
        <property type="term" value="F:RNA polymerase III core binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016480">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.50">
        <property type="entry name" value="Repressor of RNA polymerase III transcription Maf1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015257">
        <property type="entry name" value="Maf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038564">
        <property type="entry name" value="Maf1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22504">
        <property type="entry name" value="REPRESSOR OF RNA POLYMERASE III TRANSCRIPTION MAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22504:SF0">
        <property type="entry name" value="REPRESSOR OF RNA POLYMERASE III TRANSCRIPTION MAF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09174">
        <property type="entry name" value="Maf1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037240">
        <property type="entry name" value="RNA_polIII_Trep_MAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="237"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="337"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="349"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR037240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="385" mass="44276" checksum="9F3C1560E2D9D768" modified="2011-11-16" version="1">MKFIDEVDLELVNQELNFHSPDNNLVIQGGCDLFTTKPIGYDRKLFKTIDKHLDQIIEDNQLSRSLERERKNSIISIFGNSSSPPLHGEPRRGSMTERDRRASHNSANINFARSSSPVDHNDGKSLLSRSLIEDSTCEYEAVDESPFGPLKNVTTRKTFAYLIAILNISFPDQDFTNLQPTTENFYRIESPEDLIHRFNNILLSLGKKEDILNWIWDIINSYMDVIPSKSSSPYLSAQNVNHAGGHGSRKNSFNARRNSNGGSTSPSQQYLQDGCQIYQFQPSDESIFEDLTYPYQPMWSYYWFIYNKKKKRVTFLHLTAINKAHYSNVNANKEFEQKKKSQNDKIITGDDEEEYDENDEYMDSSEVAIIDEEDDNTDVVGDLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKG0</accession>
    <name>G3AKG0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_60260</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374432.1">
        <property type="nucleotide sequence ID" value="XM_007374370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKG0"/>
    <dbReference type="STRING" id="619300.G3AKG0"/>
    <dbReference type="GeneID" id="18873301"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60260"/>
    <dbReference type="eggNOG" id="KOG1165">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019279_2_7_1"/>
    <dbReference type="InParanoid" id="G3AKG0"/>
    <dbReference type="OMA" id="GRGWDYK"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000160">
        <property type="entry name" value="Casein kinase I 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="14"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW32917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="314" mass="36098" checksum="0A8BE93E9D9AFED4" modified="2011-11-16" version="1" fragment="single">MSQSSTSSIVGVHYKVGKKIGEGSFGIIFEGINILTNQQVAIKFEPRKCEAPQLRDEYRSYRILNGAKGIPSAYFFGQEGVHSILIIDLLGPSLEDLFDWCHRKFSVKTVIQVAKQMIERVESIHEHNLIYRDIKPDNFLIGKPNTPEANQIYIVDFGMAKQYRDPKSKVHIPYREKKALSGTARYMSINTHLGREQSRRDDLESLGHVFMYFLRGSLPWQGLKAPTNKQKYEKIGLKKKTTSINELCYGFPIQFAQYLTYVRNLRFDETPDYKYLIGLMDKALVSIGEQEDGHYDWMDLNNGKGWDAALNKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL26</accession>
    <name>G3AL26_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="4 14">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_137353</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4 14"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374584.1">
        <property type="nucleotide sequence ID" value="XM_007374522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL26"/>
    <dbReference type="FunCoup" id="G3AL26">
        <property type="interactions" value="540"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL26"/>
    <dbReference type="GeneID" id="18870041"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137353"/>
    <dbReference type="eggNOG" id="KOG3359">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008438_0_0_1"/>
    <dbReference type="InParanoid" id="G3AL26"/>
    <dbReference type="OMA" id="NCHLNAP"/>
    <dbReference type="OrthoDB" id="292747at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097586">
        <property type="term" value="C:dolichyl-phosphate-mannose-protein mannosyltransferase Pmt4p homodimer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900101">
        <property type="term" value="P:regulation of endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23285">
        <property type="entry name" value="beta-trefoil_MIR_PMT4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF51">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="591"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="636"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="662"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="701"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="322"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="391"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="11"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW33069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="748" mass="86130" checksum="03A54671E1C73340" modified="2011-11-16" version="1">MSSTLKKRGPKKGERVRSPSEPDDIKSKVDLPIVTPEPEPEFKYWAACIVVTTLAAWTRFYKIGTPDKVVFDEVHFGKFASYYLEGTYFFDLHPPFAKLLIAFVGWLIGYNGKFKFESIGESYIENSVPYLAYRSLSAIQGTATVPIMFLTMKTLGFSVAACIMSSIIVCFDNAHVTDSRLILLDATLNLTVAMAIYCYAKFSTYRKHPFTQSWWTWLFATGISLSLVISTKYVGVLTYVTIGIGVLHELWILLDYRRGLTLGQFAQHFFARFFALILVPFCLYLYWFYIHFAVLSKSGPGDNFMSSEFQETLLESPMAKFSKQVKYCDQITIKHKDTEAFLHSHEYDYPLRYENGRVSSNTRQVNAVIDSAEEDVNNQWEIVPIGDKKKGQDVFTNDIIRLRHVGTGGYLLTHDVASPLKATHEEFTLAFDEEEGSKKYNETLFRLRMYHASSSAKKQNQRKRVMTRATPLKIVHMDTVVAMWTHNDEVLPEWGFGQQEVCGNKKIQDKDNVWTFDTITNLAPTDVRSKYIPKTVTTMPFLKKWWELQFLMFEHNNKLSSEHPFASQPNEWPLALSGVSFYNDKKTRRQIFFVGNIPGFWLEVCLLSIYIGLLLGDQISLRRNFHLLSKKARSRLYNTLGFLFAGWAAHYFPFYLMARQKFLHHYLPAHLIAALFSGGLVEFISTNNSPTGNGRVNKYKISALVAVFSLAIIWFFFYFRPITYGDVTLTPQEVKAREWFDIKLHYST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL57</accession>
    <name>G3AL57_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein MAK16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_60407</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the MAK16 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374615.1">
        <property type="nucleotide sequence ID" value="XM_007374553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL57"/>
    <dbReference type="FunCoup" id="G3AL57">
        <property type="interactions" value="994"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL57"/>
    <dbReference type="GeneID" id="18873392"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60407"/>
    <dbReference type="eggNOG" id="KOG3064">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050888_0_1_1"/>
    <dbReference type="InParanoid" id="G3AL57"/>
    <dbReference type="OMA" id="DKGQNFC"/>
    <dbReference type="OrthoDB" id="10251342at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.110:FF:000001">
        <property type="entry name" value="Protein MAK16 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006958">
        <property type="entry name" value="Mak16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029004">
        <property type="entry name" value="Ribosomal_eL28/Mak16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23405">
        <property type="entry name" value="MAINTENANCE OF KILLER 16 MAK16 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23405:SF4">
        <property type="entry name" value="PROTEIN MAK16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04874">
        <property type="entry name" value="Mak16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01778">
        <property type="entry name" value="Ribosomal_L28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003352">
        <property type="entry name" value="MAK16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal eL28/Mak16" evidence="6">
        <location>
            <begin position="5"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="198"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="198"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="248"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR003352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="298" mass="35251" checksum="061FA7394C295657" modified="2011-11-16" version="1">MSDEVIWQVINQQFCAFKLKTDKGQNFCRNEYNVSGLCSRQSCPLANARYATVKNIGGKLYLYMKTAERAHMPNRWWERIRLSKNYNKALSQIDEHLQYWQKFLIHKCKQRLTRLTQVAITERRLALQDEERHYVGVKHKVKRREENRERKALAAAKIEKAIEKELLDRLKSGAYGDKPLNVDENIWKKVLGKVDEVDQEDEFDEEDDFELESGEEDESDYGEVEYVEDDGDDELVDVEDLERWLEQSSDESEEESDDESDEEESKKRKATSDNNKKKRRPRVEIEYEEEPIQTNIVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AL94</accession>
    <name>G3AL94_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome biogenesis protein NSA1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_135818</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the pre-66S ribosomal particle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NSA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374653.1">
        <property type="nucleotide sequence ID" value="XM_007374591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AL94"/>
    <dbReference type="FunCoup" id="G3AL94">
        <property type="interactions" value="655"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AL94"/>
    <dbReference type="GeneID" id="18869922"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135818"/>
    <dbReference type="eggNOG" id="KOG3881">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033769_4_0_1"/>
    <dbReference type="InParanoid" id="G3AL94"/>
    <dbReference type="OMA" id="IWEAKNV"/>
    <dbReference type="OrthoDB" id="18388at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22858">
        <property type="entry name" value="Nsa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037379">
        <property type="entry name" value="WDR74/Nsa1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16038">
        <property type="entry name" value="NOP SEVEN ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16038:SF4">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="393"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="413"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW33138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="437" mass="49542" checksum="730A11EF08012420" modified="2011-11-16" version="1">MKFLVSADDTGAVKEVICGRGTDTSKQSAPQPKSIKNFCREPNATRKTRVVKLEKYNYQYAIGVRIGGSVCIYEIEDEEEESKEDNKEKKEGEEEEDDNYKLVHEFKLPNVETDKPVALIKVEILESVIVAYESGKVFLIHVNEKFDFEPLQLALPSTKPISAFAMNPTKENIFAFGGEENDLQIIKLFETGVNHTIFKKKDYANAFVPSVIYRAKNVKNDHLDLRVPVWITQIIFWQDLDKGYKVITSTRYGQVRVYDTTHGRRPVGDYQLCDQPIVTMTMTEDEQDPEIIVTTTHGLVAKHSLYQVHDRAFKSNSASAGEIIKPVLQLRGKYSQGGNTGAILAAEACEDIFATAGLDRYLRVFDLASRDLLAKVYLGVEVSSLVVLDTEDDDIEEVTTEETKQVKKRTRDVEEDSEEEEEELWDQLESSSKKSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALK1</accession>
    <name>G3ALK1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">54S ribosomal protein L31, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_137154</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374759.1">
        <property type="nucleotide sequence ID" value="XM_007374697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALK1"/>
    <dbReference type="FunCoup" id="G3ALK1">
        <property type="interactions" value="104"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALK1"/>
    <dbReference type="GeneID" id="18870020"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137154"/>
    <dbReference type="eggNOG" id="ENOG502S81F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141719_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALK1"/>
    <dbReference type="OMA" id="HLVPKWT"/>
    <dbReference type="OrthoDB" id="2332379at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016340">
        <property type="entry name" value="Ribosomal_mL60"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28271">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L31, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28271:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09784">
        <property type="entry name" value="L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="13799" checksum="D3AF8A74F12F6489" modified="2011-11-16" version="1">MFGAFQGSLVRCGGYLWKKAPRLSPPQKYRLRKRMSLVDENADAIYRGLIQAQGKAIGEPSGFRKVDYLKFEMPKENEMLPKDKYTAFNKHAKNYRKKLHLFPKWTKTSFRENPEHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALU8</accession>
    <name>G3ALU8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eukaryotic translation initiation factor 1A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Eukaryotic translation initiation factor 4C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_136718</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 9">Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the eIF-1A family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374223.1">
        <property type="nucleotide sequence ID" value="XM_007374161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALU8"/>
    <dbReference type="FunCoup" id="G3ALU8">
        <property type="interactions" value="777"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALU8"/>
    <dbReference type="GeneID" id="18869995"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136718"/>
    <dbReference type="eggNOG" id="KOG3403">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109098_0_1_1"/>
    <dbReference type="InParanoid" id="G3ALU8"/>
    <dbReference type="OMA" id="RRVMMHA"/>
    <dbReference type="OrthoDB" id="6428174at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043024">
        <property type="term" value="F:ribosomal small subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001677">
        <property type="term" value="P:formation of translation initiation ternary complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05793">
        <property type="entry name" value="S1_IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000071">
        <property type="entry name" value="Eukaryotic translation initiation factor 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00216">
        <property type="entry name" value="aIF_1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018104">
        <property type="entry name" value="TIF_eIF-1A_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00523">
        <property type="entry name" value="eIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21668">
        <property type="entry name" value="EIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01262">
        <property type="entry name" value="IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1-like" evidence="11">
        <location>
            <begin position="23"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="117"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="123"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="152" mass="17131" checksum="B02F5C493DB12639" modified="2011-11-16" version="1">MGKTGKGKGGKNRRRGKNDNSGQKRELILVEEGQAYGQITKMLGNGRIEVSCFDGNKRMGHIRGKLRKKVWMGQGDIILVSLRDFQDDQCDVVHKYNSDEARTLKNMGELPETAKINETDTFGGDEDEDVNFEFGAEDDEDEEDDELDIDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM04</accession>
    <name>G3AM04_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Las1p</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_60694</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374872.1">
        <property type="nucleotide sequence ID" value="XM_007374810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM04"/>
    <dbReference type="STRING" id="619300.G3AM04"/>
    <dbReference type="GeneID" id="18873527"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60694"/>
    <dbReference type="eggNOG" id="KOG2425">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043823_0_0_1"/>
    <dbReference type="InParanoid" id="G3AM04"/>
    <dbReference type="OMA" id="WLYDNYW"/>
    <dbReference type="OrthoDB" id="10263222at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090730">
        <property type="term" value="C:Las1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007174">
        <property type="entry name" value="Las1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15002">
        <property type="entry name" value="RIBOSOMAL BIOGENESIS PROTEIN LAS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15002:SF0">
        <property type="entry name" value="RIBOSOMAL BIOGENESIS PROTEIN LAS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04031">
        <property type="entry name" value="Las1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="52944" checksum="72C0E32F1A58531B" modified="2011-11-16" version="1" fragment="single">MNQNPVIVAYKSVDDLKTVKDWFYNFTPEKDNRQRAIDKVKAWSSRARLPHGLEATSLLTSIHLSDNGSSDSSVIQLSYSMALIRFVNGLLDPFQQSNYAIPLHHLAKSVNLPSFFVELRHMATHEGLPSLPMLRIACTSALNWLYDHYWCLIEEDVTDNGDELEEDIIAKKSLTDSELLYVNETVGQVKADLDRFQVVNNLKIYKKLRKQNLDVVNRLGDNSNETATKYSKCVSKLKIFVNTKRQGLNLVGLVYPVVLARVLVHQYFIIYSHDRVQDKKLKFNPLIIKLYRPLFDELGFLFKVRFMIAIMDILVGDRKQRNVDSLIFQKLHYGEITQCDEVIQLMEWLNYFVEEALISPHTQDYTIEELNVTNRASLLKLIIEKLHVIYENLPSEIACSSMIKVVSAIISVLTNKKYKNFYKDDKRQQLETWKKHLVHLQDTKTSYELPPSLDDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AML8</accession>
    <name>G3AML8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">HECT-type E3 ubiquitin transferase TOM1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_137140</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the UPL family. TOM1/PTR1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="4"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374977.1">
        <property type="nucleotide sequence ID" value="XM_007374915.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AML8">
        <property type="interactions" value="1111"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AML8"/>
    <dbReference type="GeneID" id="18870018"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137140"/>
    <dbReference type="eggNOG" id="KOG0939">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000215_0_1_1"/>
    <dbReference type="InParanoid" id="G3AML8"/>
    <dbReference type="OMA" id="DCHFSRE"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000004">
        <property type="entry name" value="E3 ubiquitin-protein ligase HUWE1, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase UPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010309">
        <property type="entry name" value="E3_Ub_ligase_DUF908"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010314">
        <property type="entry name" value="E3_Ub_ligase_DUF913"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025527">
        <property type="entry name" value="HUWE1/Rev1_UBM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF67">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HUWE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06012">
        <property type="entry name" value="DUF908"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06025">
        <property type="entry name" value="DUF913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14377">
        <property type="entry name" value="UBM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="7 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="15">
        <location>
            <begin position="2923"/>
            <end position="3259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1910"/>
            <end position="1934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="2001"/>
            <end position="2061"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="2254"/>
            <end position="2297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="1916"/>
            <end position="1934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="2020"/>
            <end position="2031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="2052"/>
            <end position="2061"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="12">
        <location>
            <position position="3226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW33462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="3259" mass="372509" checksum="7FFE139C687BF269" modified="2011-11-16" version="1">MIIQKREHIESMQMAKPLKSLIDDLANCELRDLPVRLNANLKWERPRGDLLHWIPVLNRMDSIFEEMIKKYGLNDEFVKLQIISLQDQSLIIACLQFTHTLLEHCSDRSIYASSERVFDLINTPTVDVRLKALEVAVLIAEKFAQNNNAKYSAPKSTKQKVLELARSYPPMVPVDSAVKQQHKANIQKKDEKSSIIGDHFNFVDTLTSKKKYPSKWKYINFQYYKSSLLPLKPESHASTTTEASKKKESKKKPQKLVVSEGLNTFTMGEDVVRKLTLEQIYDKAAEVIPKEFWFNFGMHAAVAKSFNTKSYESMKLREKLLQIKCLAVGFTCNMFNVQNTSSKLFEAEPYIFSFLVDAVSPENIDRVPKDVHFTAIRILECISLKRVWGGDIIRCMGGNVSHGVLFQLIRHICKMVKEQDDDYFEEGYIHFFNMLGNMINTKSLIQKLTAGGILNDLMAFMNLHTKYRWSCSAASHLIAIYLTGSPSSLDDFVANNGFNLLIEAIGYEVDFALENPEYGGGPPKNAIVHYTISFRQANYIRNLMKLVADIIQSDVGDRLRNLFDSPLLESFNKILTHPHVFGPLILSSTVDSVFYIIHNEPTAFSILNEAKVIDTILDNYESLFLPSSELLMSLPEVLGAICLNNEGLRKIKEKKIISTYFKTFYNLPNAKELVNSDMSTNLGCSFDELGRHFPSLKPVILAETKSLIENVSSYVDDRLSGVKFYSSKIGSLYKSKDEPSQINEDGEKEIESWDTSDYAYLLDNVYFFLGGLLQDSGQWGPEAIQKIEFKTWVKFLTLKNAPYDYLLSNGISSLMGILKYFDDEKRDYGLPVVLDKLKELLDLDEVQNYIQYSSTEQSYFATLDEKSATLLFQHLNSINTILYAIAEIYINLGLMFQERFLQIIEHFGGYGLKTISDLALLLERSILEETIIRSSIPDEVSKLTSIVSTDSPPLLIHATEPSKDTPESSGTSAKFKNTLQIRFFSYYFQSYISLILACIARACTPKRQDFADIEMRRDGVEIIIELGKVFASAISRPFTNKYVKYCYILNLSNSVLYILSQKERARDTVLVSFAISLFQNGYFESLQEIAVELWNELLLMDPLVAKETAELKYISTHESSIVKNALSQVLMIFAKSVNSEIITFLPFPKLYFASGYESDVESKIISSLVTQIRGIAFKLLEETIGLNSKISDNGSGVTAANIPSPLVEQMVYIAKHVYSGKNESSEVEFIPFDVRNVFPPSDQVAYLISLGMSESQANHFFKHGLVLKDIVNKTYTDCPQMHLTVEEWDKFSEEIQSDDHDFSVDFPTYRKSKEIIDEKKRHSDMFVDEWLNIATQFPKVVAPISELFATFSTDKVVTRLGFLVSISKWRKEENSSAFGVYLHLISLLFSANADFNSFRGIFENMTTVLHSGILHDEYVNESFFPFLMSILEALLVYKDDPEPSETKHESIRYSKSNTYYRLDDDIQLKIFKQLIEIKEIRNSNSANSLARVLAMYAKNQEYSQLLRESEVVKYLIKTLRTPIKEKPISESLKTSIVLILRYAFETPEVKRNLINFDVANYFNITSTRVKDLYLCLKDQSDLIWRDAEAFEDILCKDVRLEGYSGSEFFLNKIPIKKIKKTEEDTEDVKMTDATTGESQPKRIETPTVMNILLNELMSIVKSDWLADPVEAPETKEVKKEDLFSNELFGYGCFLIQTITELLGSYKLAKFEFLTFSKKMKPNDPLKPRSSSLNFFFHQLVPTHSLEKSSGVEYERRCAISSIAKMTLLALTSTPILDEDNNPDAKKEDPDLGFIRRYVVEVLTKILKDTIQSNNLAQVRYGKLFDLFELTGLLVSTKFRDAAGPLLNNRAIKFDQFYISKVYIEKQVPNLLTSLLADLDLNFPDIERVVKAGLKPLSLLGKSKVDFQELFAGDNQGENDDDDIMPEDVDDREETPDLFRNSTLGMYDVDFNSEDEDSDMYYDEEGPLEVLSGEEIESDDESSELSAIDSDQDDVEISMDDEHASDDEYYGTIDGDVSDSSIDDIEIIDELDLGSHSDGEGEEGSSSESGLYEYEDEGDLSEYDEDELDGWIEQLEDGDHYSSDEDIADEQSAVTTMGRVERSVDLDADDESDGYSSNEGISEIEVTLDAAQGGRVRRLLDQAGLSQLDRTSPALAQLFDRLFREGGFRGTFEVSDQEDSFATNPIGRIFDNMLHLRPRRGNETGESMHIKSTRERWQDAFKMFYPKEKKDKNDLVLRVIPDIVNRIEEDSIALYKQKQSELEKIRKEREEKLKKKIEEEAKKKLEEAKQRELNAANAPPHEPVMVRIGDRDVDIGGTEIDRNFIEALPEDMREEVFTQYVRERRANATSTGEETREIDPDFLEALPGHIRDEILQQEAMTRRYASFEEGLNDDEETGDQEGEDEDEFMDFEVGGSSSSRRASTAASAKKPSKKVFFTPLVDRPGISALIRLLFSPLSINQREHVYHSLHYLCHNKQSRIEIMSLLIAIVYDCFTNQRPIEKIYTQVCSRANGSKDIKSKHGIPVGATQISVGIQIIEAIDYLLERNSHLRYYLLTEHENVFLTKKANKTFVKLGDSTKEGKFALNHLLRLLDNVLVREDQTFLDLLARVLQVSTRPLQVLKEHENESSPLQLPVIRQENYRQIIKILTGNDCSNTTFRRTISAMQNLSVLPNAQKAFSMELSDQASTLGFKIITDLNNLTKELRGTNYSSESKSFSKFSAHSSDQAKLLRILTALDYMFETKERERKQQDKSFTSEPLDEIEELTGLYKKLALGTLWDALSECLRVLEENPQLHNVANALLPLIEALMVVCKHSKVRELPLKEILKYEAKKIDFTKEPIESLFFSFTDEHKKILNQMVRVNPNLMSGPFGMLVRNPRVLEFDNKKNYFDRKLHQDKKENSKLSVNVRRDQVFLDSYRSLFFKSRDEFRNSKLEINFKGEQGIDAGGVTREWYQVLSRQMFNPDYALFTPVVADRTTFHPNRTSYINPEHLSFFKFIGRIIGKAIYDNCYLDCHFSRAVYKQILGKKQSLKDMESLDLEYTKSLMWMLENDITDVITEDFSVETDDYGEHKIIDLIPNGRNIPVTEENKQEYVKKVVEYRLQTSVEEQMDNFLIGFHEIIPKDLIAIFDEQELELLISGLPDINVSDWQSNTIYNNYSPSSIQIQWFWRAVKSFDNEERARLLQFATGTSKVPLNGFKELSGASGTCKFSIHRDYGSTDRLPSSHTCFNQVDLPAYESYETLRGSLLMAITEGHEGFGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMU1</accession>
    <name>G3AMU1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 20">Serine/threonine-protein kinase Tel1</fullName>
            <ecNumber evidence="5 20">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">SPAPADRAFT_153174</name>
    </gene>
    <organism evidence="26">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17 20">Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates histone H2A to form H2AS128ph (gamma-H2A) at sites of DNA damage, involved in the regulation of DNA damage response mechanism. Required for the control of telomere length and genome stability.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Associates with DNA double-strand breaks.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 20">Chromosome</location>
            <location evidence="2 20">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 20">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the PI3/PI4-kinase family. ATM subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5 20"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375631.1">
        <property type="nucleotide sequence ID" value="XM_007375569.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AMU1">
        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMU1"/>
    <dbReference type="GeneID" id="18870945"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_153174"/>
    <dbReference type="eggNOG" id="KOG0892">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000178_8_1_1"/>
    <dbReference type="InParanoid" id="G3AMU1"/>
    <dbReference type="OMA" id="IYMGWSP"/>
    <dbReference type="OrthoDB" id="381190at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05171">
        <property type="entry name" value="PIKKc_ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000032">
        <property type="entry name" value="Serine/threonine-protein kinase TEL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038980">
        <property type="entry name" value="ATM_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044107">
        <property type="entry name" value="PIKKc_ATM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021668">
        <property type="entry name" value="TAN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11640">
        <property type="entry name" value="TAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01342">
        <property type="entry name" value="TAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14 20" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7 20" id="KW-0158">Chromosome</keyword>
    <keyword evidence="21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11 20" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16 20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="15 20" id="KW-0779">Telomere</keyword>
    <keyword evidence="9 20" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAT" evidence="23">
        <location>
            <begin position="1837"/>
            <end position="2447"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="22">
        <location>
            <begin position="2552"/>
            <end position="2861"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="24">
        <location>
            <begin position="2863"/>
            <end position="2895"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="21">
        <location>
            <begin position="2277"/>
            <end position="2304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU365027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="EGW32355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="2895" mass="332318" checksum="F618E1278BD795F8" modified="2011-11-16" version="1">MSTLDIDKLVASLISSKIKERNDALSLLETISISKFKLSSDQFKILTQAILKLIEHESRVYYNNKSSPVESRLSLSSYYLRLLTEKSIEDTSKTLRNRIYIDLCNGIMEHFFIESGIQLEPCSIDFMKTISAILNVGYVKEHLNKKSWVTIYNFLLDIIEQSIISDADSLSLSRSNEMLFVETYNALQYLLQCDKDISVNYIEIYEDNNYFRLLSALDKTIQVLKKENIAVIMVFRIINKLIITISTVNFRFVNKMIKLGIQLMIFFNGTQWGKLHEQFLIFINLPATHKLIHLHHLPKLIGEGFIPGDILDPGLLDQEDEEESIKSQVEQGDMNKEEVLLYHIRILLQNLIKRASTSDFELKPNQIGIYLPAKSVQLNHDDWFQFKSVYLKSEDSKSWMLISGITKLLDTYFEFKSILQETSSDPNSSLFGNSMFIGNSRNKRQKLETIGDALNHSRNITEFCNRLIHSKNSTEHQILGLKIITFRLEMQKIVYNPQYNKSSQLTESTTSTEVTKTFINSNTTLDILLSNSDEGDFNRNVIFKNILGTFDNVKLNFWSLLASRAIILEEIWTESDTRTLPQYVGQILKLCFLLIKQQQTVAMACNLIFLIVFQQSNKLNELVDDSLLIQLGSLIDLAEISGPCSISEESFQYWFAIYKLSIDVNLPKKDILAVRIQDWLLSKWEGTLQNQDLDILFYSNIAEFVYWLAGNQCDYRIINYLHQLYNGSNKEVFYFTQTFRELESWLFLEPVYSTPHIIFDYNMVSLPGSISLNQIFEKVTETFKLYNSSDVSNKRYLQWLAITQRISDKFKLMDSFCSSTDGLDYHLQMGIAYFRQQNISAGECLDLIEVIVPEDLECFEMFVKAGIFEKLVNSFKSEIYSRTTSKHKVNANQTQEDGFEREFSAVRDANTPSTPASNEKMVNLLQLKYMKPHSLKVLERLGSCYKTPEELIKALVKYLEYLEGVDLLLAVLYCVKSVVPICTTISTFSKFITTIGEKLLGNQHSERNELVLTSILDLMSYVVENNQAEKQCNDISKWLIQCGERELILTESSVIAYCDLLVKQYKHMEPAKSELFHQFAKATNNVKVNLADNITVGTISGSQEDDQEIVSLYNKLFETFPDPQQSVETAGTYALFFTRLSLKSSSLFYCSIFNLLQCSRFSFFMPYLEACLQTICQQRQMKGPLELFKTAKLDILCYWWKLDDIERFPFNLFDYPDLQTFYRENYRELSAVSISTSRNITAKSNADFIENLASLKNTDSATLVSESLSLIVSVAYSSQGIRNEVFDIIQQYFQGSFKQEFKDKLSMIVLEIIKFTEIPDNKEFTDYFRGNELASIFESSFTKVDQGSVVISFKSSLELIKKLVVKYHGSANDFWNVKQVYFLIRRVSTILSVIQNPDQIKIALTKISLILVLGGYGSIDLYVTRLLVSIIRNLPVECFDYKPFVSSILVCFHDLFHHKYEQEESLPLVIQILELSFSYADIDKDLVRYIEVYTRDFEGMNSIRQFLDGIVEIYKQNDTCPKLLHSSMLESCLETLDFCQKDASVFIRLISHIFPYVGIFDHAGDRLPVVENLLRIDQAELKYFNKDFKIWIADYLSQFYLGGGALQEIKVLDKDEYNGIKGEEFEKEVSFFDFIFDKIIDQISGSDFDSAACAESILGVLIMKFQHNNKEFEKFLNFKEIYKTYKDQILSIDFHTCALLNDELDVDYLGDSLSDIIENLESFLEADLELWTTRLYLVLLQELAPYTSIAPLLSTFVIKVKSFAKESLAELICYYLVLKGKPGEKTIISILNAFVNSHNSKRSVARIFLKIITTIRIGAYKNLDKFLDVFEKVDVLSFYKLAVKNKHFRTAMMLFEDAVTLEQPGATLEDEYPSLRTVYESLDDDDLIYGLPEKPSLEYAISLEHRHGYSAELLQYSSAGFDSDITLTGKSTNYNIVESLSENGLLGVSRLINRSTGSIINCAEPFEWSWKLSQWDLPVTGTATKENEVIYKTLKQIHDYSINANDCQNMVLNVLENKLPSSESNLSIKEYRTQIIRWMKTMSEISTISSIVNYQDGKFSKFVKSYNQRTRWFEDSDLEMSENIMLARRTAFQLLSTKLKKPRSELTSNYDLSKIHLSEDEIWLGSLNDLVRYNNLTRQAGESQKMVTSTMLIDGISKSKFDNAEHSLRLNVSNLCMYQTAQMLWTQGNSSVPVLMMKELYDRGGVDIKEVGISIDKCLIRAKMVSWMSESRQELSSNIMEKHVMPVANMALSMADIQQAAKVFQLFAHFCEAQYKSKSLNEAISKLEKRVANKQKEINELKDHYGKTAVPDSEKKTVQKFYSKLKMSFNSESMDLKSLVQSRDLFSTKAVEYYLESIIVGDDVEEDLDKFFALWLEQSSNETLNSRIKASILSLPSFKLINWCIQLISRLTKDATEFQSILQELIINMCVDHPHHSLYLLISLRKHKQTAETTSNPLLLSKCSAADIIWDELLHKDDHVIENLLLPIEKFSDECVKLAAYKANKGKTLNLEKLNVGSYWLKQLPSIPPPTKFLKVDQTKAYNNVPVLVSVEHKLTIASSGLSLPKIATFNLSDGTQHKILLKHGTDDLRQDSIMEQVFNKVNNIFAQDRECKKRKLRIRTYNVVPLGPQSGIIEFVPNTTALIDIIKPYHDAHDRIKYEKAREIMKACQSKDKSERLREYERITVGLEPVLRYFFQGTFLNPDAWFSSRIKYTHGVATSSIVGYVLGLGDRHCNNILLDKNSGEPVHIDLGVAFDQGKRLPVPETVPFRLTRDIVDGFGVTGVEGVFKKSCEHTLRVLRSNRDHIISILDVLRWDPLYSWTLSPLRKKRLQDDDTNVGFQPEEDGSEAGRAVQVVADKLTAGGLSIEASVRELVQEATNPQNLALIYFGWCPFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN62</accession>
    <name>G3AN62_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_61009</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375181.1">
        <property type="nucleotide sequence ID" value="XM_007375119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN62"/>
    <dbReference type="STRING" id="619300.G3AN62"/>
    <dbReference type="GeneID" id="18873679"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61009"/>
    <dbReference type="eggNOG" id="KOG1502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_9_2_1"/>
    <dbReference type="InParanoid" id="G3AN62"/>
    <dbReference type="OMA" id="PWIFGPY"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05227">
        <property type="entry name" value="AR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="334" mass="37010" checksum="29A070BD990296B7" modified="2011-11-16" version="1">MTSTTVFVSGANGFIAQHIVKQLLAKGYTVIGSVRSHEKGEQLKQLINNDQFSYEIVSSLIDKDAFVDVLKKHPEITVFLHAASPVSFSVEDIENDLLKPAIEGTTNALNAIKEYGPQIKKVVLTASAVNIFGFGPHFDADQVYSEDVWNPITYEESLANGFFGYLGSKKYAELAARDFVAKEKVNFDISFVNPPYVFGPQAYVVKDKANLNLSNEVVNSVVKLTKGDSIPAEGSVFVDVRDVARAHIVAFENDAAASQRLFTASSRYTLDTIANIINKKFPDTTVPKGDESRNEEIYKSIHKFDNEKTNKILGFEYITLEESVSDIVDQIYNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN76</accession>
    <name>G3AN76_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ribose-phosphate pyrophosphokinase 1</fullName>
            <ecNumber evidence="4">2.7.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Phosphoribosyl pyrophosphate synthase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_61022</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="4"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375195.1">
        <property type="nucleotide sequence ID" value="XM_007375133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN76"/>
    <dbReference type="FunCoup" id="G3AN76">
        <property type="interactions" value="396"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN76"/>
    <dbReference type="GeneID" id="18873685"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61022"/>
    <dbReference type="eggNOG" id="KOG1448">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033546_1_2_1"/>
    <dbReference type="InParanoid" id="G3AN76"/>
    <dbReference type="OMA" id="CKMKKHR"/>
    <dbReference type="OrthoDB" id="413572at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000017">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000043">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000842">
        <property type="entry name" value="PRib_PP_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF57">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00114">
        <property type="entry name" value="PRPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="18">
        <location>
            <begin position="5"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="198"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="255"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="221"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="258"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00077829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW31919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="433" mass="47848" checksum="A7DF01272BFBDCDF" modified="2011-11-16" version="1">MRKCKIFVGSSHPELGKLVCDRLGVEPAPCTLKKFSNGETSVQIGVSVRDEDVYIIQSGSPHINDHIMELLILISACRGGSANKITAVIPQFPYSKQSKMKKHRGAITARMLANLLIMAGADHVVSMDLHASQMQGFFTKPVDNLFGAPTLARWIKHNIKDWNLSVVVSKNPGGTKRVTALADSLKINFAMIHTDRRRTQDGYSKRKRLQKSNTTMTVHEEQEEEGHEEEEVDNIVSELQTARIVKGHVVNEDEYLNGSVSHSRANSTNTRAKPTPLDKSHSLLDSDVLGGTYDAAESDDDEDEPESLAQEKLITLVGDVKDKVAIILDDMIDKPTSFIAAAEHLRLNCGAKAVYLIATHGVFNDSCLETLTESKCIDKIVVTNTYPIDTECMEKFSEKLTVIDVSPIFAECIRRDHFGESISVLFDSLAAIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANG1</accession>
    <name>G3ANG1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61065</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375226.1">
        <property type="nucleotide sequence ID" value="XM_007375164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANG1"/>
    <dbReference type="GeneID" id="18873701"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61065"/>
    <dbReference type="HOGENOM" id="CLU_1994029_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANG1"/>
    <dbReference type="OrthoDB" id="4084571at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442620" evidence="1">
        <location>
            <begin position="30"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="125" mass="14368" checksum="CDE76F9CCBC072C9" modified="2011-11-16" version="1">MVASPPPQSFGSKLFGVVLSLAVGCFVISKWGPDEDYFVKIPYTPEEIEQRKKEGKGFSIKLVDKGTTPYPRKSEIKSEAQKLALEAWSNKVGRIQEEMSILYKKRQKELRRRAVDKLIEQHRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANL5</accession>
    <name>G3ANL5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_153619</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375819.1">
        <property type="nucleotide sequence ID" value="XM_007375757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANL5"/>
    <dbReference type="STRING" id="619300.G3ANL5"/>
    <dbReference type="GeneID" id="18870959"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_153619"/>
    <dbReference type="eggNOG" id="KOG1502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_9_2_1"/>
    <dbReference type="InParanoid" id="G3ANL5"/>
    <dbReference type="OMA" id="HGGNLWM"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="4"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="334" mass="37292" checksum="6B9D11D583EB0F0A" modified="2011-11-16" version="1">MTAVLVTGANGYISQHIIQLLLQQNYTVVGTVRSVAKKEKYAALFSSCNLTIEVVPKLDTPHAFDFVFGKHRDITVVLHVATPVKSYAVNYDSEIISPAINGTTYLLESIRDVAPQVTRVVYTSSVAALAHPSVIYSTRGTFSERDWNQVTYEQSKESPRLAYYYAKRIAEQTAWEFANSRVNFSLSTVLPGGTFGPLVFDSDVADPMPSTSDYPIRLLKLRRDDPIPEFGAGFIDVRDLAQAHILVMESEAARGKRVVVEAEMVDNHIMLDIIRRNFPQFRDKLPECNPDSSQFIEPDIAQSRKILSGLEFTPISKTVVDAVTQVLRARESDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANL6</accession>
    <name>G3ANL6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_67149</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375821.1">
        <property type="nucleotide sequence ID" value="XM_007375759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANL6"/>
    <dbReference type="GeneID" id="18875143"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67149"/>
    <dbReference type="HOGENOM" id="CLU_912662_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANL6"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="305" mass="35279" checksum="C1294F91D72E20FF" modified="2011-11-16" version="1">MSVKLVFNNKNKTKFNCRLPQLNEMNEEDEQDKYIEGLKNSSNDSNMSLYFIVGDEETTKIRALEEFKFKYDEFLRHELTHPDLEFWNKVRRFSQECFQKYEAFMHRSAQRVDLYVQAFSLLKSGFENYLTEERVQKLIKGYFKRNIVDNESGLVKSGSSKNIREIGRSFTLEYIKVLENISSNPQIYGNPVTIHQMFGLYDLDVGPLDNTKILKLAAIGSFKPWFDSHYKGFFKEFNESYKKQKTALESILPWKGLISAVVGVGICCTFCFGLCKYTSYELEELAKAVIANVTAATANTITTDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANN6</accession>
    <name>G3ANN6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_61629</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375840.1">
        <property type="nucleotide sequence ID" value="XM_007375778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANN6"/>
    <dbReference type="STRING" id="619300.G3ANN6"/>
    <dbReference type="GeneID" id="18873981"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61629"/>
    <dbReference type="eggNOG" id="KOG2045">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_565210_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANN6"/>
    <dbReference type="OrthoDB" id="372487at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.750:FF:000002">
        <property type="entry name" value="5'-3' exoribonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041385">
        <property type="entry name" value="SH3_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041106">
        <property type="entry name" value="XRN1_D2_D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047008">
        <property type="entry name" value="XRN1_SH3_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18129">
        <property type="entry name" value="SH3_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18334">
        <property type="entry name" value="XRN1_D2_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exoribonuclease Xrn1 D2/D3" evidence="3">
        <location>
            <begin position="2"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="5'-3' exoribonuclease 1 SH3-like" evidence="2">
        <location>
            <begin position="153"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="394"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="474"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="483" mass="53861" checksum="B5536C01EE03356A" modified="2011-11-16" version="1">MVQDKNSKINIGLELKFETKREKVLGYTRKSNNGKFWEFSPLAITLINSYKTKFPQLFAKLSALSSKDIPKVAEISSSEEIKEVRSWLKEAKADLIPVSLESESLTKFSFRAIEQYMETYILDQVPTVDRAIRGVPRDAILNASESYQLLGDQRFELGDRIVYVQDFGKVPIMSKGTVASILTVGSKTSLGVIFDHPLLGGSDMNGKLTSKRGLVIDSSLVLNLTNRQYVYHSRASKSRKPLTPEQQAARVKAVEHKKVEQDHKRVEQEQKRIEQDQKKSEQDKEKQNLIKQESQKQTHELLSLLKIKSDSKAAEHEQSSGDKDEQAVTNPNAIKHIYGQIYSSVMNQGAAPPQPMPLPPQPPVGMPIPIPRGNGMGMPYGYIPAVPGIPIPPQFLQQQQQQQQQPSQVVNPNGEDQQAINQQEKGESQEHRGSEGQRRGRGGHRVAHRGRGAHRGAVHSRGGHSRGRGGKGKPKSESDPEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANU5</accession>
    <name>G3ANU5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Deacetylase sirtuin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_61129</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375306.1">
        <property type="nucleotide sequence ID" value="XM_007375244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANU5"/>
    <dbReference type="FunCoup" id="G3ANU5">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANU5"/>
    <dbReference type="GeneID" id="18873730"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61129"/>
    <dbReference type="eggNOG" id="KOG2684">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021544_4_0_1"/>
    <dbReference type="InParanoid" id="G3ANU5"/>
    <dbReference type="OMA" id="THKFIAH"/>
    <dbReference type="OrthoDB" id="2919105at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF8">
        <property type="entry name" value="NAD-DEPENDENT HISTONE DEACETYLASE HST3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="6">
        <location>
            <begin position="12"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="337"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="365" mass="41498" checksum="3ACA88555B364DE7" modified="2011-11-16" version="1" fragment="single">MISLDLTQEDLPTDTSIRLNEVIKCIAKAKKCTVLTGAGISCNAGIPDFRSSDGLYNLVKQRHPSKITRGQDLFDISLFRDEISLSLFCTFMESLYKSSLKATATETHKFIKVLKDKNKLLRCYTQNIDCLESTMNLNLGINLKEFDNSKFNEVWKNLDVVQLHGNLHKLSCTSCFSNFDWNEKFQSMLSQGLNPECSVCYAKYEERLYSGKRLTGNIGVLRPDIVLYGENHPQSELLSQGLNVDLRSKPDLLIIMGTSLKVDGVKRLVKCLSKSIHEKGGKVIFINKTPLSKQWKNVIDYEVLTDCDDFVRILKREIPDLFLTQEQLDSKKLNNPITEQLPAVKREPKVKSEPKVKQEIPKSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANV5</accession>
    <name>G3ANV5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_51123</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375856.1">
        <property type="nucleotide sequence ID" value="XM_007375794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANV5"/>
    <dbReference type="STRING" id="619300.G3ANV5"/>
    <dbReference type="GeneID" id="18871520"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51123"/>
    <dbReference type="eggNOG" id="KOG2294">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_928027_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANV5"/>
    <dbReference type="OMA" id="GCVINIM"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22701">
        <property type="entry name" value="FHA_FKH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45881">
        <property type="entry name" value="CHECKPOINT SUPPRESSOR 1-LIKE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45881:SF1">
        <property type="entry name" value="FORK HEAD PROTEIN HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FHA" evidence="7">
        <location>
            <begin position="69"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Fork-head" evidence="8">
        <location>
            <begin position="182"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="182"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="300" mass="34013" checksum="5B02A96B0CCFB709" modified="2011-11-16" version="1">MPTVPRLNHTNTRIDPGSVDSILEILQEPEQRTLVADKYANSKIIDAEVKAYAKLAGSDWTYYIKKLSINIGRSSPHDGPKQDNVDIDLYPNPAVSRQHAIISYNLHVRCWELKVVGRNGIKVDGHKNSTSVVALRSGCVINIMGTEIMFIEANSNITISPRFAALVESINRKSVLLKDDTPPPYTYTTMIAQAILADQEGLLPFDKIYSWILNHYPYYECTRTTTSLHKSIAHTLNTNKAFESVNENPSDKTLAKWQINEKYRNDLLSYMSLEYQFTKFPTIANFQDASLRLSIRKSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANZ8</accession>
    <name>G3ANZ8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Type 1 phosphatases regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_51159</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Regulator of type 1 phosphatases which maintains protein phosphatase activity under strict control.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the YPI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375899.1">
        <property type="nucleotide sequence ID" value="XM_007375837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANZ8"/>
    <dbReference type="STRING" id="619300.G3ANZ8"/>
    <dbReference type="GeneID" id="18871526"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51159"/>
    <dbReference type="eggNOG" id="KOG4102">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098333_3_0_1"/>
    <dbReference type="InParanoid" id="G3ANZ8"/>
    <dbReference type="OMA" id="YQPHYEN"/>
    <dbReference type="OrthoDB" id="307488at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011107">
        <property type="entry name" value="PPI_Ypi1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20835:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PPP1R11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20835">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PPP1R11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07491">
        <property type="entry name" value="PPI_Ypi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="84"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="141" mass="15816" checksum="386CC1306913E524" modified="2011-11-16" version="1">MSNNSSRTELTPVSEQPVLRLRPSNKQNNEETKEGTSSSSPKKMRKKKKKARVQWTEDVVDNENMNKKKTKICCIFHPNRTFDEEVEHDHSHSDPSSSSSSDESDLSEGEGGGGGDNEVKPNAYEHQPVYENQSKIPPSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP24</accession>
    <name>G3AP24_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal lysine N-methyltransferase 4</fullName>
            <ecNumber evidence="1">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_138237</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that monomethylates 60S ribosomal protein L42.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SETD6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375332.1">
        <property type="nucleotide sequence ID" value="XM_007375270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP24"/>
    <dbReference type="FunCoup" id="G3AP24">
        <property type="interactions" value="278"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP24"/>
    <dbReference type="GeneID" id="18870107"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138237"/>
    <dbReference type="eggNOG" id="KOG1338">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017135_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP24"/>
    <dbReference type="OMA" id="WEGLIIC"/>
    <dbReference type="OrthoDB" id="5312224at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1410.10:FF:000007">
        <property type="entry name" value="Ribosomal lysine N-methyltransferase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011383">
        <property type="entry name" value="N-lys_methylase_SETD6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF34">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE SETD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011771">
        <property type="entry name" value="RMS1_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="24"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR011771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="483" mass="55703" checksum="A6C5F27536D5A354" modified="2011-11-16" version="1">MADFSARTDKFIAFLQENDVTISPKLQIADLRAANQGRGIIALEDIEIDETLFTIPRTVLINSLNNSLVQDQPELADKLAGLENEWDALILVLLYEYKRKESKWTDYFNVLPDLDTFEFHELLFWNDEQLSDLKPSLVLDRIGKDKTVEMYERLVAIVNQWNLEELKGMTMEEFTKIATIIMSYSFDVAQGTEDEDEDEDDEEEEEEVEYIKSMVPLADTLNADTHLNNAILTYNKNQDLVMTCIKPIKKGEQVYNTYSDHPNCEILRRYGYVETTGSKYDFGEIPLTLITSHFSKSLSEQLVFDLLEIITEISQEEQEDDEPEVVAESYDCYSSKEVNLTFVFLIQLVTIIALIHKQEPITADIQNITRIYKKCYQLVESKKVTTEFVKNYTEILQARINEYPPIAKEPYDKELKEPLRKSMAEVVLKSEYQSLSNCLDVDATLANTLDGSVKTIDDDKLIRNILKKRSNEDISSNAKRTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3APC0</accession>
    <name>G3APC0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61176</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphatidylethanolamine-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375373.1">
        <property type="nucleotide sequence ID" value="XM_007375311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APC0"/>
    <dbReference type="FunCoup" id="G3APC0">
        <property type="interactions" value="1469"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APC0"/>
    <dbReference type="MEROPS" id="I51.001"/>
    <dbReference type="GeneID" id="18873761"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61176"/>
    <dbReference type="eggNOG" id="KOG3346">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043994_3_1_1"/>
    <dbReference type="InParanoid" id="G3APC0"/>
    <dbReference type="OMA" id="NWGYGTP"/>
    <dbReference type="OrthoDB" id="2506647at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030414">
        <property type="term" value="F:peptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030162">
        <property type="term" value="P:regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046578">
        <property type="term" value="P:regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001858">
        <property type="entry name" value="Phosphatidylethanolamine-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF148">
        <property type="entry name" value="CARBOXYPEPTIDASE Y INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01220">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="157" mass="17810" checksum="01E35395AB20CE0E" modified="2011-11-16" version="1" fragment="single">MPLITISQSLDESYTKHKVIPEVVDEFDTQGLLTIEYSKDEHVTLGNQLPVANTQHVPEIQLTLNSPTQDGKIESITENDKFILVMTDPDAPSNTDHKWSEYLHWLVTDLQLTNGEAKEGEQINHKIDVSQGRELVKYMGPGPPPKTGKHRYVFLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3API7</accession>
    <name>G3API7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61241</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375434.1">
        <property type="nucleotide sequence ID" value="XM_007375372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3API7"/>
    <dbReference type="STRING" id="619300.G3API7"/>
    <dbReference type="GeneID" id="18873794"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61241"/>
    <dbReference type="eggNOG" id="ENOG502SBFU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_836769_0_0_1"/>
    <dbReference type="InParanoid" id="G3API7"/>
    <dbReference type="OMA" id="RYYLIHG"/>
    <dbReference type="OrthoDB" id="422106at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019416">
        <property type="entry name" value="NCBP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16291">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16291:SF0">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10309">
        <property type="entry name" value="NCBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="337" mass="39614" checksum="C01E97B6BD87FE72" modified="2011-11-16" version="1">MSEQPEVAPYGGFITRTLDVEKEREEKLKEQAIEVNVNSHTEIIRPEAIYITGVDSLSTDDIKAFVDYYLNYNVIETVNEDETKNITYEPLPFDDQIQFKVEWINDSSVNIRFDTHEESAKALHKISITQGNPDVIDTDEPPVISFTDPQYINNIIQLRESKPYTPVIQFRKQQSLVNRLGVKPEAETESTEKTENTENAESAPVEESAMEEDESSVVLYTRQSFQSDRKVKNAAQYSRYYLIHGEPERKPYRKHKRRREPKPEPVEEQEPEEDLFADRLSFIENRKSREPSARAEQFDNEEDLFADKLRQRERSRSPVRSAQRSNYRHRSNRYRER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3APK5</accession>
    <name>G3APK5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16 17">Phosphatidate cytidylyltransferase</fullName>
            <ecNumber evidence="6 16 17">2.7.7.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_61264</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 16 17">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + CTP + H(+) = a CDP-1,2-diacyl-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.7.7.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 16 17">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 16 17">Belongs to the CDS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="6 16 17"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375452.1">
        <property type="nucleotide sequence ID" value="XM_007375390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APK5"/>
    <dbReference type="FunCoup" id="G3APK5">
        <property type="interactions" value="751"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APK5"/>
    <dbReference type="GeneID" id="18873805"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61264"/>
    <dbReference type="eggNOG" id="KOG1440">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023471_1_1_1"/>
    <dbReference type="InParanoid" id="G3APK5"/>
    <dbReference type="OMA" id="FFAYMYF"/>
    <dbReference type="OrthoDB" id="10260889at2759"/>
    <dbReference type="UniPathway" id="UPA00557">
        <property type="reaction ID" value="UER00614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070319">
        <property type="term" value="C:Golgi to plasma membrane transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006658">
        <property type="term" value="P:phosphatidylserine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000374">
        <property type="entry name" value="PC_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016720">
        <property type="entry name" value="PC_Trfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13773">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13773:SF8">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE, PHOTORECEPTOR-SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01148">
        <property type="entry name" value="CTP_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018269">
        <property type="entry name" value="PC_trans_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01315">
        <property type="entry name" value="CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 16 17" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14 16" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="15 16" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR018269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW32176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="438" mass="50050" checksum="5E5B8E1FE9E8B86F" modified="2011-11-16" version="1">MAQSSKKDNSVVTEKPDTSSKTPSINNVKNSKPEKESSKVVVNEKEKKKQAFITRTIWTFVMIGLFFLLLSSGHFALILMVLLFQILTFKEIIGLVSEPARDKKIPYNRSLNWYFVAATWYYLDFPSFVDFFQDSIYSNKYLTLLVLNHKFICYSLYIAGFVFFVWTLKKGYYKFQFAQLCVTHMALLLIVFQAHLIISNILRGIIWFFLPAALVIVNDVFAYICGITFGRTQLIEISPKKTVEGFVGAWICTGIATIIFSYILSRSDYMICPAQNLSTHLYNYPHCEPNPVFIPHIYQLPQNIIDLTGWEIISFRPLYFHTAILATFASLIAPFGGFFASGLKRAFGIKDFGDTIPGHGGITDRFDCQFIMGSFTYLYYQTFISNSNLNLGKILQLAIINLSTPQLIQLVKSLMRYLNREHVIPDDNLSAIFDLLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3APN6</accession>
    <name>G3APN6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_61290</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dpy-30 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375483.1">
        <property type="nucleotide sequence ID" value="XM_007375421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APN6"/>
    <dbReference type="STRING" id="619300.G3APN6"/>
    <dbReference type="GeneID" id="18873822"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61290"/>
    <dbReference type="eggNOG" id="KOG4109">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115916_1_1_1"/>
    <dbReference type="InParanoid" id="G3APN6"/>
    <dbReference type="OMA" id="WLNEHVT"/>
    <dbReference type="OrthoDB" id="417678at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22965">
        <property type="entry name" value="DD_DPY30_SDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007858">
        <property type="entry name" value="Dpy-30_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049629">
        <property type="entry name" value="DPY30_SDC1_DD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05186">
        <property type="entry name" value="Dpy-30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="49"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="128" mass="14169" checksum="588FEC2D615A8579" modified="2011-11-16" version="1">MNSEIEQTNNSEPTSVTATTSNGVGANSATATTTTTTTTPDVDNIQVKQEQRRPKLEPSRESSMDTTPLTNQQPMHEIVGGSSVRRYLNEHLTKHLLEGLKEVSQAKPEDPLRYLGEFLINRSEELSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3APW2</accession>
    <name>G3APW2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_139132</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375559.1">
        <property type="nucleotide sequence ID" value="XM_007375497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APW2"/>
    <dbReference type="FunCoup" id="G3APW2">
        <property type="interactions" value="313"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APW2"/>
    <dbReference type="GeneID" id="18870181"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139132"/>
    <dbReference type="eggNOG" id="KOG0064">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007587_5_0_1"/>
    <dbReference type="InParanoid" id="G3APW2"/>
    <dbReference type="OMA" id="DIQAGHF"/>
    <dbReference type="OrthoDB" id="422637at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015867">
        <property type="term" value="P:ATP transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015916">
        <property type="term" value="P:fatty-acyl-CoA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042758">
        <property type="term" value="P:long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF69">
        <property type="entry name" value="PEROXISOMAL LONG-CHAIN FATTY ACID IMPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="435"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="47"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW32283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="684" mass="77570" checksum="E411333C7F54CC0A" modified="2011-11-16" version="1">MSEQQVGFNQQNLANSLLKVYRSNRSILLNTSYIILMATAFAGATSTTSASKSTEEEEKEEANMGEPTKKKHPKLSKRSLKRLAKAIVPTLTDKTILYLSGHMVLLVIRAILSIKVANLDGQLVGALVSRRLKVFSKLLLYWMLIGVPASLTNALLTWSKQKLRRAIRININKDIIDNYLPDNLDANYYSLIHLTGNKIRDPNQRITTDVSRLSDDLSSLPGQLLKPTLDLILCARELSRSGVGNGEGTLALGMLAHFSTIVLRLFAPPFAKLAAERAHLEGQLRSSHSKIVSNNEEIAFLRGHVRELDYIDYCYYTLERFLKGEYWKKAIHEIAQTFIVKYFWGAAGLVLCSAPIFINKYLGEEIDRNSAGNFITNRRLLLGASDSLDRLMYSRKYLLQIIGHASRVSDFQDTLNEVTSRKKSSGNVKYQNDQITFDSVRLLTPADVTLIPNLTFSIKSGDHLLIAGPNGSGKSSMFRMLGGLWPVKEGTITIPTSADMFYLPQRAYLCSGTLREQIIYPHPLSKQKKSTEELKEILTLLKLDEHIDELDEVKNWDEELSIGAQQRLAMARLYYHEPKFAVLDECTSAVSPDMEQFMYRHAQDLGITVLSVAHRPALWHFHRYLLKFDGKGGYYFGKLDAEKRLKYEQERILLEKNLRDLPVLKERLLELKMVSNVQHEKYGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3APZ6</accession>
    <name>G3APZ6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">P/Homo B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_50884</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S8 family. Furin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375593.1">
        <property type="nucleotide sequence ID" value="XM_007375531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APZ6"/>
    <dbReference type="FunCoup" id="G3APZ6">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APZ6"/>
    <dbReference type="MEROPS" id="S08.070"/>
    <dbReference type="GeneID" id="18871496"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50884"/>
    <dbReference type="eggNOG" id="KOG3525">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002976_2_1_1"/>
    <dbReference type="InParanoid" id="G3APZ6"/>
    <dbReference type="OrthoDB" id="300641at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007323">
        <property type="term" value="P:peptide pheromone maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000026">
        <property type="entry name" value="proprotein convertase subtilisin/kexin type 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000005">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF14">
        <property type="entry name" value="NEUROENDOCRINE CONVERTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 14" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442546" description="P/Homo B domain-containing protein" evidence="17">
        <location>
            <begin position="19"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="771"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="P/Homo B" evidence="18">
        <location>
            <begin position="494"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="683"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="685"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13 14">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13 14">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13 14">
        <location>
            <position position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW32317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="792" mass="87334" checksum="95AFB7A1B0D3D83A" modified="2011-11-16" version="1">MLLYTILLLLYFISISLQRRLLPQRDYEHNNYFLVELDTSSSEKPLLDFIKKYKSDYVFDHQLPIDNYYVFKINKTHRHNSFLVDLASNNFAHSFNRKSGFDDLYNDFINNHALKSVHLLPPKRLQKRRPVQVDPAELDAISRARRNQLIEPAKQKLEEVASKLNINDPTFGHQWHLINTISLGNDINIADLWLEGKFGEGIVTAVVDDGLDSTSEDLRDNFNFEGSWDFNDNSPLPLPRLFDDSHGTKCAGEVSAVKNNFCGIGVAFKSQVSGIRILSGSLTDADEAAAMVYGLDINDIYSCSWGPTDNGKTLSEPHPIVKEAILKGVIEGRDSKGALYVFASGNGAKFDDSCNFDGYTNSIYTITVGAIDYKGSHPDYSEGCSALMVVSYSSGSGVYIHTTDINQQCSSMDHGGTSAAAPLASAIYALVLEANPELTWRDVQYITVLTAVPINEDDGSYQTTSIGRKYSPKYGYGKLDATKMVHFAEEWKNVKPQAWYYSDLIIVTETITYDKKVIESSVFVSEEDLKLANLERVEHAAVKVNIKSSYRGAVGMRLISPEGVISDLATFRKLDDASTGFRDWTFMSVAHWGESGVGEWKVEVFGEADITFVDWQLRLFGESIDAGKTTKFSLRTDYADVQRELKNQNPANDQPKSSSTTETIKYISSSFKVSEYEVLPVSSIKSPLPEPSTESSQQSSSELSSSSPTTTATKSLSSSSSALSSSSILSTLATVSVLSSLAISSTPILSSSISEPPTFTLSSSISEPSSLSVLTSISLAIAIFLTIFFIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ48</accession>
    <name>G3AQ48_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Actin patches distal protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_61961</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376172.1">
        <property type="nucleotide sequence ID" value="XM_007376110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ48"/>
    <dbReference type="FunCoup" id="G3AQ48">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ48"/>
    <dbReference type="GeneID" id="18874124"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61961"/>
    <dbReference type="eggNOG" id="ENOG502RYPV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033921_1_0_1"/>
    <dbReference type="InParanoid" id="G3AQ48"/>
    <dbReference type="OMA" id="CTIKYPA"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03062">
        <property type="entry name" value="TRX_Fd_Sucrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009737">
        <property type="entry name" value="Aim32/Apd1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902">
        <property type="entry name" value="ACTIN PATCHES DISTAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902:SF14">
        <property type="entry name" value="ACTIN PATCHES DISTAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06999">
        <property type="entry name" value="Suc_Fer-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="294" mass="32781" checksum="559B05A1D8E9572D" modified="2011-11-16" version="1">MTILNRLWGKPDPTTEIKQQGFKISECSETCESCSSKFPSSLKFEEGASLWKSTKPYGLHIVVSTGKSDWPHDATGVRGTLSNKVSKWADDSKNPKKATLGNVKVTCSSLGSDELLVDDEYMTEKKGDILLLPYFIWVKGITITEVDSTLDKLIEMLVDKKPIPDIITAIPKVQVDPSKAYVFLCSHKSRDKRCGLTAPIMKKEMDLYLRELGLYRDFGDSSPGGVNVAFINHIGGHKFAANVIIYLKSEGKNIWLALCKPNNVKPIIDECIEGGGKVWPDKVRLVQKFDPIEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQI9</accession>
    <name>G3AQI9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Spindle pole body-associated protein Vik1/Cik1 microtubule binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_51539</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376314.1">
        <property type="nucleotide sequence ID" value="XM_007376252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQI9"/>
    <dbReference type="STRING" id="619300.G3AQI9"/>
    <dbReference type="GeneID" id="18871565"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51539"/>
    <dbReference type="eggNOG" id="KOG0239">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_449032_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQI9"/>
    <dbReference type="OMA" id="CISIKNG"/>
    <dbReference type="OrthoDB" id="4089036at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031852">
        <property type="entry name" value="Vik1/Cik1_MT-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16796">
        <property type="entry name" value="Microtub_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spindle pole body-associated protein Vik1/Cik1 microtubule binding" evidence="3">
        <location>
            <begin position="379"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="126"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="201"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="309"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="55"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="643" mass="74150" checksum="D53716D00060AF3C" modified="2011-11-16" version="1">MSSTNLPTPTNKRVLSERSPNEPSQSTISKKQRLLYSQMLPPDRKFESKIPAPKYTTTITQSSVQDPQTSTPTRNSPSFPKDYQQTVLSIKDRLARKSSLNDPSTFLKPKPPQTKRPSGGDIQEKYYALNEECKNVSSEVDQLTRELAEIKRKSKSLQYKVDDATDEVQLMRDRFQFTEESVVKKVADEEKLMFLKSQESQMKLENHYKELEFEMKNQLEEAKSFDNQFLLDKIEELKTTEQNLAEEIEKLKSEKEQLLMSEQHSMEQELQKRLSLVKQDQEKSSDELQVANEKKKLIQSKFTISKEYLDQQQANLQKLKSEIGEVEASMSIFLSEKESLLSELSTIESNLDAAKEIDEKEQSEFNSLEQIHSKALAKMYKHDTHRRILENSIMDYEGKIRVYVVSNEEETKEIQSRGKLYEFNKQFPKNIPASVIADEFSCLLKSSLRGSNVSIINTAHSIPTSELVLLTYNHISDPRSSTKNRQFTIRYQCISIQNLQVFDNLDSKKNVSSQGIIQQMQASKIQIEDKNHIESIVQTIPKQVEQELVVHLFSVETGSSDKQYETTVMIVDLGNIQRPDQMELLQTFKLTTNSDSPTTKIFSFARYNSKCLFLGSADSDYDYEYLDELQLLNSMDSLYKPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQR2</accession>
    <name>G3AQR2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_62219</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376387.1">
        <property type="nucleotide sequence ID" value="XM_007376325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQR2"/>
    <dbReference type="FunCoup" id="G3AQR2">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQR2"/>
    <dbReference type="GeneID" id="18874222"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62219"/>
    <dbReference type="eggNOG" id="ENOG502QRI8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029881_1_1_1"/>
    <dbReference type="InParanoid" id="G3AQR2"/>
    <dbReference type="OMA" id="WAPIREP"/>
    <dbReference type="OrthoDB" id="5584247at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052246">
        <property type="entry name" value="Cell_Polariz_PKAAnc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13155:SF1">
        <property type="entry name" value="A-KINASE ANCHOR PROTEIN 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13155">
        <property type="entry name" value="A-KINASE ANCHOR PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="417"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="449"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="507"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="3">
        <location>
            <begin position="40"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="531" mass="61100" checksum="4757693D2979B212" modified="2011-11-16" version="1">MTHNSYNEKYQSNYPNSHYLANLDNLNATNQTIHLNRLPTLGEILANKSKSPVDLLTFYQFMEEVEGKVDYLDFWFDLVNHLNLCKHYVKGLRDSIVRHSQIYSPKDRDLSGSTGITAPSGGIRDSVAASDTSKRKSLSSSILLELIINDHILEENDSPRLSAFLRGDINLENPDPKLNELIQQYTTELQKSPKLIQPGAQISPKFASSQKSSPNSATMPSPATGLNSPRVAKRVDSKSRLVSGEDEERIGGDSYSESFYQTHFEVSQADRAVSPSQYIAQQQKRDPINPFVEQQQQQQQQQSQLHHHAKLAQITPNLLENLLKDVTMEENAFISRDRLRESSHNLLLKYFMGDSEKNLNLPERINSSIIKAIEVEERDDPEVFNHVKNYVFNRIENEHLPKFLDCMATRNINHSEFWRVLIGFFFIFIGFWVSYIFIFLNYRKSLRPVILVPYIIGFFLIISSIYLIDPVLAWFGLSEGFSKTSQSRLIKIRERFIYRFILKRSHWVLFLILLATAILTVLFSLVPGHRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR57</accession>
    <name>G3AR57_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Letm1 RBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_61808</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376010.1">
        <property type="nucleotide sequence ID" value="XM_007375948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR57"/>
    <dbReference type="GeneID" id="18874061"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61808"/>
    <dbReference type="eggNOG" id="ENOG502S645">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1016226_0_0_1"/>
    <dbReference type="InParanoid" id="G3AR57"/>
    <dbReference type="OMA" id="NSQANIW"/>
    <dbReference type="OrthoDB" id="73691at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="31926" checksum="E179010118E975FC" modified="2011-11-16" version="1">MAQKLSSDRIEYRARNSRATGDVKRSTGFDYEQTITPELFNISRSQYQLLKRTPHDVIRLPLFALIALIFEELTPLICYVFPEITPRTCVIPPLLPKIWSSKPIKTLTTETIHMNETKLADLSLKTAYNLEPHRVKLLARALGLTSKIVPTSLYPISYLRNQLQHHYNYLQVDNYYLCGFNSQANIWNLTNQELLLACLERNLIFDIKLDVAQFNELIDDASKQAFEKKYFDDLRYKLLQFLIDFPNANIGYLCITHSISNRKEQTDKLVELFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR60</accession>
    <name>G3AR60_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">S-methyl-5'-thioadenosine phosphorylase</fullName>
            <ecNumber evidence="4">2.4.2.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">5'-methylthioadenosine phosphorylase</fullName>
            <shortName evidence="4">MTA phosphorylase</shortName>
            <shortName evidence="4">MTAP</shortName>
            <shortName evidence="4">MTAPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MEU1</name>
        <name evidence="6" type="ORF">SPAPADRAFT_61810</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>S-methyl-5'-thioadenosine + phosphate = 5-(methylsulfanyl)-alpha-D-ribose 1-phosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:11852"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="EC" id="2.4.2.28"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.28" evidence="4"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376014.1">
        <property type="nucleotide sequence ID" value="XM_007375952.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR60"/>
    <dbReference type="FunCoup" id="G3AR60">
        <property type="interactions" value="425"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR60"/>
    <dbReference type="GeneID" id="18874062"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61810"/>
    <dbReference type="eggNOG" id="KOG3985">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054456_0_1_1"/>
    <dbReference type="InParanoid" id="G3AR60"/>
    <dbReference type="OMA" id="ADPFCPE"/>
    <dbReference type="OrthoDB" id="431409at2759"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00873"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09010">
        <property type="entry name" value="MTAP_SsMTAPII_like_MTIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1580:FF:000008">
        <property type="entry name" value="S-methyl-5'-thioadenosine phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01963">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010044">
        <property type="entry name" value="MTAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018099">
        <property type="entry name" value="Purine_phosphorylase-2_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01694">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679:SF2">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01240">
        <property type="entry name" value="PNP_MTAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 4" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="5">
        <location>
            <begin position="21"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="70"/>
            <end position="71"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="103"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="244"/>
            <end position="246"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="4">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="4">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW31236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="327" mass="35699" checksum="8EAB3B146C13BE08" modified="2011-11-16" version="1">MSPERVPASQLPHHYDGKVTLAVIGGTGLYDLPNLHPVARLTISTPWGFPSGAITIAHTESGFPVAFLARHGPHHDLLPSDVPSRANIAALKSIGVKAIIAFSAVGSLQPEIKPRDFVLPTQVIDRTKGIRPSTFFEKGFVAHAMFGEPFDLQLNKLIRDAVDSNGFLDGFGNDHTPVFHTKEKTNNGEDLTIICMEGPAFSTRAESRLYRSWGGSVINMSVLPEAKLAREAEIAYQMICMSTDYDSWNESEEPVTVETVVGNLKANSTNACKLAAHLIDAIATHPVELGKDLEGTMKFAVSTNPNGVKKELLEKMHFLFPGYWPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARC2</accession>
    <name>G3ARC2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Agglutinin-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_140900</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ALS family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376507.1">
        <property type="nucleotide sequence ID" value="XM_007376445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARC2"/>
    <dbReference type="FunCoup" id="G3ARC2">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="GeneID" id="18870330"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_140900"/>
    <dbReference type="eggNOG" id="ENOG502RGCG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031316_2_0_1"/>
    <dbReference type="InParanoid" id="G3ARC2"/>
    <dbReference type="OMA" id="PYSTINC"/>
    <dbReference type="OrthoDB" id="3981162at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903561">
        <property type="term" value="C:extracellular vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030446">
        <property type="term" value="C:hyphal cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030445">
        <property type="term" value="C:yeast-form cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044403">
        <property type="term" value="P:biological process involved in symbiotic interaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030448">
        <property type="term" value="P:hyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2430">
        <property type="entry name" value="Agglutinin-like protein, N-terminal domain, N2 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008966">
        <property type="entry name" value="Adhesion_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008440">
        <property type="entry name" value="Agglutinin-like_ALS_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024672">
        <property type="entry name" value="Agglutinin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043063">
        <property type="entry name" value="Agglutinin-like_N_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033504">
        <property type="entry name" value="ALS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011252">
        <property type="entry name" value="Fibrogen-bd_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793:SF2">
        <property type="entry name" value="AGGLUTININ-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793">
        <property type="entry name" value="ALPHA-AGGLUTININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05792">
        <property type="entry name" value="Candida_ALS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11766">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01056">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49401">
        <property type="entry name" value="Bacterial adhesins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0134">Cell wall</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 16" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0843">Virulence</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442676" description="Agglutinin-like protein N-terminal domain-containing protein" evidence="16">
        <location>
            <begin position="18"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Agglutinin-like protein N-terminal" evidence="17">
        <location>
            <begin position="53"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW31729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="457" mass="48492" checksum="B856421D29787253" modified="2011-11-16" version="1" fragment="single">MLRFFIFSFLVLATATADQVSGIFTSFDSLVWQKAANYPFAGPAYPSWISTLSWAIKGTDVSPGDTFTLTLPCVFKFTTSQTTVSLSVGSTNYATCTFNPGDVVVAFSQLQCVVSDSVTPSTDAHGTINFPVAFNVGGSALSTDLQDSTCFADGTNTVSYYDGDNKLSTSVQFTGGYNGSPSQIIYTNRVVPSLNKQQHMVIAGDCPAGYRSGSLGIQIANSGPKIDCSSIHIAITNALNAWYLPENADTDFSYTYSCSTTSFTINYQNIPAGYRPFIDTLVSVATGSSITTTYVNNYVCANSILTTNNGKTVSWGNYDNNVVGGNGQAVEVVTHTYTGSTTQVSTMPFDSTKDQTKTIVVDVPVPTTTVTSTYIGVSTSFTTHSASPGSTATVIEYDPVHTTITVSTCSPQSGSSITFVRRAEPGATDTVYVYTGECPVTHTEVETTTEATTESTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARL1</accession>
    <name>G3ARL1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_141336</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376542.1">
        <property type="nucleotide sequence ID" value="XM_007376480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARL1"/>
    <dbReference type="STRING" id="619300.G3ARL1"/>
    <dbReference type="GeneID" id="18870365"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141336"/>
    <dbReference type="eggNOG" id="ENOG502SX3X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095379_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARL1"/>
    <dbReference type="OMA" id="NCAEIQQ"/>
    <dbReference type="OrthoDB" id="4072599at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="183" mass="21297" checksum="6C85F6D8D378BC75" modified="2011-11-16" version="1">MWPFTSSKPTEGKKTEDINKELPKDLQEFFKQENPDKKHANLFEQSSPQNHIFEVLNKHKDDPKDPRLAYYKSSQDINNACAVNCSEIKNQVVECSKQVTWLQSGSPCADIIKAMSACTQIQKTALQTLHFDECYNVKQCQMMRYYVDKIFTENFGQYGSEMNDETRAKFNKDIDDAVYKVWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARL7</accession>
    <name>G3ARL7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]</fullName>
            <ecNumber evidence="8">1.5.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Saccharopine reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_62366</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-saccharopine + NADP(+) + H2O = (S)-2-amino-6-oxohexanoate + L-glutamate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57951"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.10" evidence="8"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376548.1">
        <property type="nucleotide sequence ID" value="XM_007376486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARL7"/>
    <dbReference type="FunCoup" id="G3ARL7">
        <property type="interactions" value="184"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARL7"/>
    <dbReference type="GeneID" id="18874290"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62366"/>
    <dbReference type="eggNOG" id="KOG0172">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016207_3_1_1"/>
    <dbReference type="InParanoid" id="G3ARL7"/>
    <dbReference type="OMA" id="WNYKFTW"/>
    <dbReference type="OrthoDB" id="10059875at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004755">
        <property type="term" value="F:saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000008">
        <property type="entry name" value="Alpha-aminoadipic semialdehyde synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000072">
        <property type="entry name" value="Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1870.10:FF:000002">
        <property type="entry name" value="Saccharopine dehydrogenase Lys9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1870.10">
        <property type="entry name" value="Domain 3, Saccharopine reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051168">
        <property type="entry name" value="AASS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032095">
        <property type="entry name" value="Sacchrp_dh-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133:SF22">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16653">
        <property type="entry name" value="Sacchrp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="11">
        <location>
            <begin position="5"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Saccharopine dehydrogenase-like C-terminal" evidence="12">
        <location>
            <begin position="122"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="445" mass="48431" checksum="BC427D6C7041CCD1" modified="2011-11-16" version="1">MVQKVLLLGSGFVAKPTVDILAQTSGVEVTVACRTLTKAQALAGSVAKAISLDVTDEAALDAQVAQFDLVISLIPYTYHVNVVKAAIKNKKHVVTTSYINPQLKALEPEIEAAGITVMNEIGLDPGIDHLYAVKTIEEVHAAGGKITSFLSYCGGLPAPENSDNPLGYKFSWSSRGVLLALRNSAKYWKDGKIVDVSSEDLMASAKPYFIYPGYALVAYPNRDSTTYKELYNIPEAETVIRGTLRFQGFPEFIKVFVDTGFLKDETSEIFSKPGPWNEATAQLVGAASASEADIIAKINQLTKFKSPEDQERIISGMKWLGLFSSKTITPRGNPLDTLCATLEELMQYEEGERDLVILQHKFGIEHANGEKEVRTSTLVDYGDPKGYSSMAKLVGVPCAVATQQILDGTLSKRGLLAPMSSDINDPIMKTLKDVYGIYLVEKTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS03</accession>
    <name>G3AS03_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62462</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376630.1">
        <property type="nucleotide sequence ID" value="XM_007376568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS03"/>
    <dbReference type="GeneID" id="18874330"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62462"/>
    <dbReference type="HOGENOM" id="CLU_2777540_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS03"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="69" mass="7964" checksum="20DBD7D4C6015824" modified="2011-11-16" version="1">MAAKRKNGPHFRVWICEWKLASISAFAVCRLGDTCNLTHYLLRPNCELATCMEDTLKFDSTPENFALKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS74</accession>
    <name>G3AS74_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Alpha-1,3-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_141907</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377066.1">
        <property type="nucleotide sequence ID" value="XM_007377004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS74"/>
    <dbReference type="FunCoup" id="G3AS74">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="GeneID" id="18870407"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141907"/>
    <dbReference type="eggNOG" id="ENOG502RZ48">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015387_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS74"/>
    <dbReference type="OMA" id="CANGEIG"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000033">
        <property type="term" value="F:alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392:SF1">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW31033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="738" mass="85862" checksum="9429DB5D86EAC493" modified="2011-11-16" version="1">MTRHSKSNTRLFFYLVVITWFISALTWISVIQFLPSQTHSVNFSSTSNDWEQAVPENKLELPSFKEDPIIKLLQADLQDSSNLETELHSSVYDQIFKNHEISSVLSNLDFNQRCDLYFRNLYVADQNWKLDPDTQFNIDYNFEEFLEFRHKHYDEFKQEFYANRGGRDVKELANFIREKYNKAKENDHVQTIVEYLSTLRIFNKCYVTNDNRTQIEATKSFVNRQKTQILEAPAFVETKRERMTDFKSVGLNAFEHRVYPWLSFEYPLYEHWSGATFFDPPTLKTGQGYWGKRSTSKTYSSFLSEFKNKCNGKGLVLTMPDSHVDTVVNLIHLLRALKNTLPIQIVNFEPLSFDTKQKLVTAAQSDFITLPKSYDKVAKHLGSDYLGPGGLPKQEIWFVSVANAIDRHYRDKFKYFGNKFLATIFNSFEEFILIDDDTVMLQSPEYFFNIKKYQETGALMFRDRTSAFRKSDDCEFLKRLGPATIDSIMFDIPIMTDYSISRPFFDKLYHYIEAGLVVLNRNIHFNSMLVISQINFFSPISSKVYGDKELFFLGFAFNGDENYYVNTLGAASVGNLTDPQERLGPDDKPLRSQELCSSHPGHISDEDGHTLLWINSGFANCHYADIVDYSKEITFNTGLKFLPQDEDSFKKHYSSPLRIRNAIIPPYDGSIHVNVLGEPMRGWDQHKYCRGYTWCAYDIVGGLSTMGKVNRIQGSLIEYKEWEQNLFDYYADIWVGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASN4</accession>
    <name>G3ASN4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Translation machinery-associated protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_142498</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2 5">Interacts with the 40S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="5">The SUI1 domain may be involved in RNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the DENR family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376753.1">
        <property type="nucleotide sequence ID" value="XM_007376691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASN4"/>
    <dbReference type="FunCoup" id="G3ASN4">
        <property type="interactions" value="1238"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASN4"/>
    <dbReference type="GeneID" id="18870454"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142498"/>
    <dbReference type="eggNOG" id="KOG3239">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073511_0_1_1"/>
    <dbReference type="InParanoid" id="G3ASN4"/>
    <dbReference type="OMA" id="EVFEIDM"/>
    <dbReference type="OrthoDB" id="277199at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11607">
        <property type="entry name" value="DENR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050318">
        <property type="entry name" value="DENR/SUI1_TIF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046447">
        <property type="entry name" value="DENR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005873">
        <property type="entry name" value="DENR_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048517">
        <property type="entry name" value="DENR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01159">
        <property type="entry name" value="DRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789:SF0">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21023">
        <property type="entry name" value="DENR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="SUI1" evidence="7">
        <location>
            <begin position="93"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="63"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="199" mass="22492" checksum="27A98DA3B78842CE" modified="2011-11-16" version="1">MTELAPKSIVYCGICSWPPEYCEFGVSRARCQSWLDTNHHDLYEKIYTEDITNQTSTLSLEKQEKLNNDLAKAQHKQELKQERELQKMLNSKIVIKRIERNKRKHIISISGLEVFNLDSKKLAKTFASKFATGASVTKNADKTEEILIQGDVSDEAKEYIEKLLQDQGLNDIKVEQIDDKKKKKKDAAAAAAATTAGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASX9</accession>
    <name>G3ASX9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA sliding clamp PCNA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PCNA</name>
        <name evidence="11" type="ORF">SPAPADRAFT_56738</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involved in DNA repair.</text>
    </comment>
    <comment type="function">
        <text evidence="7">This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processivity during elongation of the leading strand.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the PCNA family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376794.1">
        <property type="nucleotide sequence ID" value="XM_007376732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASX9"/>
    <dbReference type="FunCoup" id="G3ASX9">
        <property type="interactions" value="1134"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASX9"/>
    <dbReference type="GeneID" id="18872064"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56738"/>
    <dbReference type="eggNOG" id="KOG1636">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
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        <property type="project" value="GOC"/>
    </dbReference>
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        <property type="term" value="C:nuclear replication fork"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="C:PCNA complex"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="C:site of double-strand break"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:error-free translesion synthesis"/>
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        <property type="term" value="P:error-prone translesion synthesis"/>
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    </dbReference>
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        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:subtelomeric heterochromatin formation"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="entry name" value="Proliferating cell nuclear antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.70.10.10:FF:000001">
        <property type="entry name" value="Proliferating cell nuclear antigen"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.10.150.10">
        <property type="entry name" value="DNA Polymerase III, subunit A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
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        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
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        <property type="entry name" value="Pr_cel_nuc_antig_CS"/>
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    </dbReference>
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        <property type="entry name" value="pcna"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
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        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PCNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PCNA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA N-terminal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA C-terminal" evidence="10">
        <location>
            <begin position="127"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00054163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000641"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="259" mass="29133" checksum="B019EEAAEC11C58F" modified="2011-11-16" version="1">MLEGKFDQSTLLKKIVDAIKDCVKLCNFNCTEHGITVQAVDDSRVLLVSLLVGQTAFDDYRCDRDITLGIDLESFSKIIKTANNEDYLTLMADDTPDSVHTIFEDKKKERVSEYSLKLMNIDSEFLKIDDMEYDSVINMPSYEFAKIVRDLKNLGESLVITVTKDSVKFSSEGETGNGSVVLKPYTDMDKPDESVKVTLDSPVDLTFGSKYLNDIIKATSLSDVITIKLADKTPALFEYKLDAGGYLRFYLAPKFDEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT85</accession>
    <name>G3AT85_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PRA1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_62743</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PRA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007376818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT85"/>
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        <property type="interactions" value="280"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AT85"/>
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    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62743"/>
    <dbReference type="eggNOG" id="KOG3142">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103851_1_1_1"/>
    <dbReference type="InParanoid" id="G3AT85"/>
    <dbReference type="OMA" id="HASIMDK"/>
    <dbReference type="OrthoDB" id="63113at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="Prenylated_rab_accept_PRA1"/>
    </dbReference>
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        <property type="entry name" value="PRENYLATED RAB ACCEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRENYLATED RAB ACCEPTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="175" mass="19252" checksum="A450FC49252D9C0A" modified="2011-11-16" version="1">MSQFLNLASFGNVGSRFNLDRIRSDFSSVGTSISKLRPPQEFFDFRRLSKPANFGEIQNRVGFNLGYFSANYIAIVLILSVYALITNVLLLFVTCFVVGGVYGINRLQGQDLSTPFGKYNTSQLYTGLLIVAIPLGFLASPISTMMWLIGSSAVCVGSHAALMEKPIETVFEEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATE6</accession>
    <name>G3ATE6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL40</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_62821</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL40 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376942.1">
        <property type="nucleotide sequence ID" value="XM_007376880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATE6"/>
    <dbReference type="FunCoup" id="G3ATE6">
        <property type="interactions" value="252"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATE6"/>
    <dbReference type="GeneID" id="18874480"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62821"/>
    <dbReference type="eggNOG" id="KOG4778">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126124_0_0_1"/>
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    <dbReference type="OMA" id="DYAYKAP"/>
    <dbReference type="OrthoDB" id="2098203at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3440:FF:000001">
        <property type="entry name" value="Mitochondrial ribosomal protein L40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019192">
        <property type="entry name" value="Ribosomal_mL40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042831">
        <property type="entry name" value="Ribosomal_mL40_fung"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39150">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L28, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39150:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09812">
        <property type="entry name" value="MRP-L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="144" mass="16766" checksum="326A4FC8EF904C1C" modified="2011-11-16" version="1">MFKSFAKPQSSSGVLTFIRGKRISATNPATERIVQQLSVLAAGRKQPKLLNLSNEDIVKHRTIMSAWKLFTRKKKRAEQEQLNQQYESICNAMNDLKETSPALFELASHKEKRLRFPLEMRFPTEYPPNRPWVYNFTGPVKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATE9</accession>
    <name>G3ATE9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_62822</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376945.1">
        <property type="nucleotide sequence ID" value="XM_007376883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATE9"/>
    <dbReference type="FunCoup" id="G3ATE9">
        <property type="interactions" value="413"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATE9"/>
    <dbReference type="GeneID" id="18874481"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62822"/>
    <dbReference type="eggNOG" id="KOG2355">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057592_3_0_1"/>
    <dbReference type="InParanoid" id="G3ATE9"/>
    <dbReference type="OMA" id="NEQHNSD"/>
    <dbReference type="OrthoDB" id="6512918at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00267">
        <property type="entry name" value="ABC_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43158">
        <property type="entry name" value="SKFA PEPTIDE EXPORT ATP-BINDING PROTEIN SKFE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43158:SF2">
        <property type="entry name" value="SKFA PEPTIDE EXPORT ATP-BINDING PROTEIN SKFE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="14"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="331" mass="37634" checksum="F26971E3EBA5AE70" modified="2011-11-16" version="1">MNNADNTKDTSLAIETHKLTYTFPTSHKIGLQDITLEIPWGTTNLIIGPNGAGKSTLLKILAGKTLIKSGLLKIGGFDPFEFRSNRNEQHNSDINSYITYLGTEWAANSVVKRDIPVNLLLSSVGGEVYAERRNRLIDILDIDPSWSMLNVSDGERRRVQIAMGLCKPWRLLLLDEVTIDLDVVVRSRLLNYLREECHQRQCTVIYATHIFDGLGHNWFDRVIHLDAGMMLDDLKQSEIKFVNDNNGNGVKIEGGAHIHPAHSVSITKAESLHPLALHWLYDDLERRGTREDEKEKMAQRHNDWVNTRDGKYFDAEDDVLVKYFKATRSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATH8</accession>
    <name>G3ATH8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_62852</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376974.1">
        <property type="nucleotide sequence ID" value="XM_007376912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATH8"/>
    <dbReference type="STRING" id="619300.G3ATH8"/>
    <dbReference type="GeneID" id="18874492"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62852"/>
    <dbReference type="eggNOG" id="KOG3010">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049344_1_2_1"/>
    <dbReference type="InParanoid" id="G3ATH8"/>
    <dbReference type="OMA" id="FRPHYPP"/>
    <dbReference type="OrthoDB" id="10027013at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051052">
        <property type="entry name" value="Diverse_substrate_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942">
        <property type="entry name" value="METHYLTRANSF_11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942:SF4">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="44"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="307" mass="34564" checksum="B5156073652954B5" modified="2011-11-16" version="1">MSTFSKSNFKTLHYNTFRPQYPPSFYSILSKYVARPLPLQTTIDLGCGTGVATYPLLNISKNVIGIDLSPSMIDTANSLKSSRVKELGYADSSIRFITGAVEEFVAKDEHVGQVDLITAAQCIHWFQDYDAFFANAAKLLRPGGVLAYFYYIDPIIVNFSGSGSDKADVIKKAHDTYLKYAYDDPKLMGPHWEQPGRDILKHFCTTVNDHIPGLYDDITINTFIPSAEKPYADEQVDLDLKKTGLKLQGYVDYLGTYSGLHNYVEATGDTTFLDRFLSELEEVTGWDRETTTVDLVWNTGYTFIRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATP4</accession>
    <name>G3ATP4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ndc10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_68392</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377241.1">
        <property type="nucleotide sequence ID" value="XM_007377179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATP4"/>
    <dbReference type="STRING" id="619300.G3ATP4"/>
    <dbReference type="GeneID" id="18875215"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68392"/>
    <dbReference type="eggNOG" id="ENOG502SBAB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_387313_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATP4"/>
    <dbReference type="OMA" id="MWDDFKG"/>
    <dbReference type="OrthoDB" id="4016214at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.20">
        <property type="entry name" value="Centromere DNA-binding protein complex CBF3 subunit, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038279">
        <property type="entry name" value="Ndc10_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031872">
        <property type="entry name" value="NDC10_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16787">
        <property type="entry name" value="NDC10_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ndc10" evidence="2">
        <location>
            <begin position="32"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="493"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="493"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="633" mass="73707" checksum="848D31A45C6EA8F7" modified="2011-11-16" version="1">MMSDSEDSSSDPNEYSIEDYYKLVRPLWAARNINANYLNNHKLLRTLVDFSLSHSLPLKNLQKRELELGDIIPTKINYVDTLIIKSKNTTDNEGLFQYSGCFRHQFVEFCPHLAISAYLFSRFHIPDEYGSFEFILSDATKRISLEEVKLLKGNNKLSAISYSQQHKSSINALSISGLNYKDINLTKLLSTQDFEYTEKAVSRTLDGLPHQIMLQLAGFDSFQNYNIPRNAIEPHQELLDSIFPFVNQITPEFYNEDMLRIKELLIMLRRSLCQDMVVIKQKYPLNPLSKSDIFNSREFEKFSKQVEDAGELDGWVHDSCFSPNDTNEDLPHDNWPVFQDPDSKAELQRVIEFQMSKLRSLDNQVSLYAQEQSHMYSVLSKFIETQNEVFQRQSEYMQRVQNSINGLVILMSSKNKNTIPLAQQSLNETRNFINTVESTNLKQGLDTTIELLAKLNSSQMQQYQLQQQQAHQLQQTQPHSSVIPITNNHPVFNIHAATSPSSKSLSPDPQSQTPMSPVQLERQRVLNRRLSRQATTLFEMWDDFKGLEKELKDHEITVTEWLKVHGSSERQFRHTRLKIIKFIEDEAQRRGTTVEYVKERLHNKMRNRLRPWTLDEVQRMLTSGKRINLDDNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATW4</accession>
    <name>G3ATW4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome c oxidase assembly protein COX15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_63195</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Fe(II)-heme o + 2 A + H2O = Fe(II)-heme a + 2 AH2</text>
            <dbReference type="Rhea" id="RHEA:63388"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:61715"/>
            <dbReference type="EC" id="1.17.99.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:63389"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Porphyrin-containing compound metabolism; heme A biosynthesis; heme A from heme O: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377311.1">
        <property type="nucleotide sequence ID" value="XM_007377249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATW4"/>
    <dbReference type="FunCoup" id="G3ATW4">
        <property type="interactions" value="919"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATW4"/>
    <dbReference type="GeneID" id="18874636"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63195"/>
    <dbReference type="eggNOG" id="KOG2725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017627_4_1_1"/>
    <dbReference type="InParanoid" id="G3ATW4"/>
    <dbReference type="OMA" id="AFVCYSW"/>
    <dbReference type="OrthoDB" id="1726137at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120547">
        <property type="term" value="F:heme A synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016653">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003780">
        <property type="entry name" value="COX15/CtaA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023754">
        <property type="entry name" value="HemeA_Synthase_type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23289">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23289:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02628">
        <property type="entry name" value="COX15-CtaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="404"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW30340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="478" mass="53268" checksum="C15CF87A4E217353" modified="2011-11-16" version="1">MLSKSRFFFTKNSIVLNTLAQNKGCRLTLNQLQCNTPTKSIFSTIRSRFIPGLKTTKRPFGTRSYTIATADATTISDIPKIKLSIPKPESPSTSPRSVGWWLVGTSGLVFAIVVIGGLTRLTESGLSITEWKPVTGALPPMNQEEWLAEFDKYKESPEFKQLNSHISLEQFKFIYSMEWGHRQLAVFFWKSGRFTPHITKRVIGLTGLLGLQGAIGWWMVKSGLDEEQLAERNSKPTVSQYRLTTHLGAAFAMYLGVLWTAFEILNENKYIKQAKTTPEALKTILEQLNNPVVKKAKVIGIALLVLTYVTAMSGGMVAGLDAGLIYNTFPHMGDDWIPNKGELMSPVYSRKPDQSDLWWRNLLENPTTVQLVHRIFATTTFFAILAAHMFVNKNKTSIPKKAKMVMHTTMGLALLQVSLGITTLIYLVPIPLAAAHQAGALALLTASLAFSAKLKKPRPELVSYVNNYMKSQLLKSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATY3</accession>
    <name>G3ATY3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_143339</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377330.1">
        <property type="nucleotide sequence ID" value="XM_007377268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATY3"/>
    <dbReference type="FunCoup" id="G3ATY3">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="GeneID" id="18870537"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143339"/>
    <dbReference type="eggNOG" id="ENOG502QUDU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075335_1_0_1"/>
    <dbReference type="InParanoid" id="G3ATY3"/>
    <dbReference type="OMA" id="YFGICVN"/>
    <dbReference type="OrthoDB" id="4089394at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000747">
        <property type="term" value="P:conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033481">
        <property type="entry name" value="Dni1/Fig1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28092">
        <property type="entry name" value="FACTOR-INDUCED GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28092:SF1">
        <property type="entry name" value="FACTOR-INDUCED GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12351">
        <property type="entry name" value="Fig1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="254" mass="27965" checksum="3AA284F231506F53" modified="2011-11-16" version="1" fragment="single">INMLNKSLTLIVVIMQFIASVLLSFLVLGCIDTSKRYSDNFLLQYQFNNTSPLFQHLNTTGNSTISKTDAFRDIKVSVSYYGVCLKLTKGQFNCTNYNSLRSFPDYSIAVVNKTKLDLVQLAHRFSSVCHPKVLLATILLSLVALLLLCYVVIPSLPGKFQLKKGLVGLTGFTAMFWGIGAMLQHQAVSASTVIIREGSMGLIKTEIGKRAESITWVSFGLDILVFGGVMVDFVTEYKSRRVSGPPAMFAPGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU50</accession>
    <name>G3AU50_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Golgi apparatus membrane protein TVP38</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Golgi apparatus membrane protein tvp38</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_143324</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Golgi membrane protein involved in vesicular trafficking and spindle migration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TVP38/TMEM64 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377397.1">
        <property type="nucleotide sequence ID" value="XM_007377335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU50"/>
    <dbReference type="FunCoup" id="G3AU50">
        <property type="interactions" value="112"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU50"/>
    <dbReference type="GeneID" id="18870535"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143324"/>
    <dbReference type="eggNOG" id="KOG3140">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041954_1_0_1"/>
    <dbReference type="InParanoid" id="G3AU50"/>
    <dbReference type="OMA" id="KWQALET"/>
    <dbReference type="OrthoDB" id="166803at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000022">
        <property type="term" value="P:mitotic spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051076">
        <property type="entry name" value="Golgi_membrane_TVP38/TMEM64"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47549:SF1">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47549">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP38-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="99"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="11">
        <location>
            <begin position="116"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW30426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="344" mass="38609" checksum="9C894DA33507EB1D" modified="2011-11-16" version="1">MPRLHTNSDVHFISPTNTSASGMTSLQRLIAQRMNTVVQFTHHSSTWYAKQSSLTQVALGIFVVLATTAFILMLIFHADIIKFLIYVSDQWHSLKYGSVILFILVFMVGFPPLIGFTALSMLTGMIYGFPQGWPLLASASVLGCIASFFIYRYILQAHAQRFTNKNETFRALGECMTDDNSLVLLVLIRLCPLPYSLSNGALSSIPNLSASTYIIATVITSPKLLIHLFVGHKLKSLGDETRSGTTKFVDLLSILITLTAASATAYIIYFKMQKKLASYHQRRYVNDDVLVFGNFDDDLEMNENTTEVELNSADFDADNFIIEDEDFEHEHEHIPEHNKDVSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUA2</accession>
    <name>G3AUA2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63301</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007377387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUA2"/>
    <dbReference type="GeneID" id="18874693"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63301"/>
    <dbReference type="eggNOG" id="ENOG502S195">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037539_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUA2"/>
    <dbReference type="OMA" id="MNDYVTF"/>
    <dbReference type="OrthoDB" id="2163387at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="445" mass="48316" checksum="FFCF0B27933D9060" modified="2011-11-16" version="1">MEVEHPLYEDNTMTNEIPATSTSTTTLTNHAKLNKTPLKKEELEKIARELKKKLSKASITAKQSLSPTNIRTTPQSKLNRSSPLKKIMSSTTKEISPIRENFPSSSPITYNSLNNSTVSNLYSPTGRSPTHIKTPAAMYLASSPLKHTNGDAIGLDDSPLKRRKKSSPSVKMVLQEISPKEASIQHALKPSLDMTNNKEDAEQTGNPGTTTPTLSKQELYNGLKTPTQRHQMLSRNGTDAVSHQQQQQQHQQQQQGQQGQHPQQPGAYNDAEGADLLMYLATSPSPAKPYFQSNALSTPRTSMQRQVQPTQPPQFLAPLPPASAASTISNSSTAPPPPVTPKRTANINRDTPSSRLTPGGFLSAMSGGQGLGLPSQGLTLTPTGFNMNDYVNFFTPSPGSAAARNLLRTPDFNNLINGGNGTPGMRGKIEGKMLNFNKVLFRDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUA3</accession>
    <name>G3AUA3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein QOR3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">QOR3</name>
        <name evidence="2" type="ORF">SPAPADRAFT_63302</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377450.1">
        <property type="nucleotide sequence ID" value="XM_007377388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUA3"/>
    <dbReference type="FunCoup" id="G3AUA3">
        <property type="interactions" value="227"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUA3"/>
    <dbReference type="GeneID" id="18874694"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63302"/>
    <dbReference type="eggNOG" id="KOG1198">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_16_1_1"/>
    <dbReference type="InParanoid" id="G3AUA3"/>
    <dbReference type="OMA" id="EDIEYGI"/>
    <dbReference type="OrthoDB" id="9992527at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08249">
        <property type="entry name" value="enoyl_reductase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047122">
        <property type="entry name" value="Trans-enoyl_RdTase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348">
        <property type="entry name" value="HYPOTHETICAL OXIDOREDUCTASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348:SF2">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C2E1P3.01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="1">
        <location>
            <begin position="8"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="361" mass="39578" checksum="56E97811C153ACE0" modified="2011-11-16" version="1">MKVAVVSGSLEPNQLTKVADIELPPIKPNQLLIKSEAFAINPTDWKHIVSHKSKPGNIAGCDVSGTVAEVGSEVTGFKVGDHVSSFIHGNTQDFVGAFGEYVIANNKAIINYGRELKHSDNTEPGTIDSFEGAASVTLGLDTVALSFAYELGIDYDKTKNKDKFILIWSGATATGILAIQVAKLIYGLQVITTASPKNHEFLKSLGADYLFDYKDAEVVDKIKEVGKGKIVYGLDTIASDETFQKLYDATEGAPEVFLDSLLGKDAKHIVTDPKRVVHYGHTVAYLAFDKEYILGDGIKVYQEPEMVTKFDEFWTKYLPKYIHQIKHSHLLILKPGLESAAEGLELLRQGKISAKKVVFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUD9</accession>
    <name>G3AUD9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">WD repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">WDR6</name>
        <name evidence="8" type="ORF">SPAPADRAFT_157643</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat WDR6 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377486.1">
        <property type="nucleotide sequence ID" value="XM_007377424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUD9"/>
    <dbReference type="FunCoup" id="G3AUD9">
        <property type="interactions" value="637"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUD9"/>
    <dbReference type="GeneID" id="18871090"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_157643"/>
    <dbReference type="eggNOG" id="KOG0974">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002615_0_1_1"/>
    <dbReference type="InParanoid" id="G3AUD9"/>
    <dbReference type="OMA" id="GGAHRQW"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002130">
        <property type="term" value="P:wobble position ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051973">
        <property type="entry name" value="tRNA_Anticodon_Mtase-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14344">
        <property type="entry name" value="WD REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14344:SF3">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="190"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="650"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="973" mass="109191" checksum="276D7DEC73751890" modified="2011-11-16" version="1">MSGFNQISSLNEVEFAPLHHYGPVTALKIYKNYVLAGYGPILKIYQMTQGDLLDVAEVFCKQVFSRNKIHHIEITSQGRLCLSGARSFMVLDLEEVLQGKGNFKERAINEWIVTCAFYENWLLILNSHNVVYKVDLASFEFDKVHCQEKSILYSGSIQVLDNGTVYVAAGTVMSGVIIWDLHSRKIVHNLTDHEGSIFGVKVDKTGKYIVSCSDDRSIKLYDFASGTVLATGWGHGSRIWTLEFFKGDNVKIMSTGEDCSLRLWQYQEGNSQLVQLEMVENCHSGKHIWSGDVDDLEYQVCVTGGADGRIKLHDLNKVNRTEYSLQEISSQTGLVFGKKDFIRDYFELKDRLIVLISNGDLVEYSDNSFKTIAFNDQHKLKDFAIVKGFSDINIVIIAARNGDLLIADFTSDLPQLSWIQDEYLGSNKLTNLLVYSTDNKYYVLVDVPNPNIPFILREFDSSFNLVNVTQLSQPNQTSFTTTDMVVDSVNNWLVIASRYVTIAAYDLSTTSMALFKKLSPGDTITSVQIITSKTNELALLILVKDGSYLYTTLTLSTTFHLDVTHANKLTKGFIEGGYIKSNKLVLYGFKSSYFYIWNETCQFEIMNELCGGSHRQWKLAPSKFIYVKDKTTLVIRNIAHRFVNDGLIHLGTHGREIRDVAVSPDGKLLVSASEDATIAVSQLEDGVKGIWSMNNHISGLQRVKFLNSEYMASSAANEEFIIWKVTQSNIPLVKEFVRLKPSAVENPDLRIMNFASIELESNVFILVTVYSNSNIKIWRIDLNKEHYGDMVKLLVNDFYTTCCILNVELLQKDDKTWLMIGATDGYVTIWDVTSKLDPEEHKLGASIIHQQLHQNGVKAMEVFTHQDGYRLITGGDDNALVVCDLQVPDLKLDIVGYEESAASATITSISVVGEHKFVTTSVDQIVRIWEYNEGLTCLSARYTTIADTGCSASMGSTIYIGGAGLSSWKVNNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUJ8</accession>
    <name>G3AUJ8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NADPH-dependent 1-acyldihydroxyacetone phosphate reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_57303</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377525.1">
        <property type="nucleotide sequence ID" value="XM_007377463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUJ8"/>
    <dbReference type="STRING" id="619300.G3AUJ8"/>
    <dbReference type="GeneID" id="18872111"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57303"/>
    <dbReference type="eggNOG" id="KOG1209">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_9_1"/>
    <dbReference type="InParanoid" id="G3AUJ8"/>
    <dbReference type="OMA" id="NTACMET"/>
    <dbReference type="OrthoDB" id="2102561at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000140">
        <property type="term" value="F:acylglycerone-phosphate reductase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169:SF6">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="29974" checksum="A4AD51F8490962E8" modified="2011-11-16" version="1">MSETKYALVTGACQGIGYQLALQLSKAGYKVIGCSPPSVDEIKTTALTVNEITGGKLHVLYNNAGIAVIGPGIETSEEDLTNVFNVNVIGHINMTKYFAPQVINAKGTILYTSSVAARVPLAWISAYNATKAAIDQYALTLHGELKPFGVRVHSVITGGVNTAIFDPNLPAKFEDSYYNVPGAEASFKASATMSRDLNISPEKYAKQIVADIQSRCDPGFNLYHGAKAYLLHCASRILPFWLFEMAVQIYFKEYQVFRTIAKNVKQGDYKKKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV20</accession>
    <name>G3AV20_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphoribulokinase/uridine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_144289</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377860.1">
        <property type="nucleotide sequence ID" value="XM_007377798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV20"/>
    <dbReference type="FunCoup" id="G3AV20">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV20"/>
    <dbReference type="GeneID" id="18870611"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144289"/>
    <dbReference type="eggNOG" id="KOG2702">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067202_1_1_1"/>
    <dbReference type="InParanoid" id="G3AV20"/>
    <dbReference type="OMA" id="QNAHYID"/>
    <dbReference type="OrthoDB" id="6362633at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442658" description="Phosphoribulokinase/uridine kinase domain-containing protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="2">
        <location>
            <begin position="61"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="256" mass="29075" checksum="805AD7DD003D2DD9" modified="2011-11-16" version="1">MFPRWGYTIALTSALWLFFSTIITHTHTDTSTVHVKKSHLDKIVKRVYEKYHSVDNRRRLVISLAGIPGSGKSTLAQRLLPRLERYFNTVVLPMDGFHLSREELARMDDPQEAFARRGAPFTFNPRGFIDVIKKISDPAYTDTVYAPSFDHAVKDPVDGGVTIGPDAQVVIVEGNYVSLKDEVWDQIESLVDDTWFLQCDLGLAKRRIVRRHIEAGISSDEQEAIDRAEGNDLINARYIIENSKKTKLVIVDTDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV59</accession>
    <name>G3AV59_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pyridoxamine 5'-phosphate oxidase Alr4036 family FMN-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_63482</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377628.1">
        <property type="nucleotide sequence ID" value="XM_007377566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV59"/>
    <dbReference type="FunCoup" id="G3AV59">
        <property type="interactions" value="162"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV59"/>
    <dbReference type="GeneID" id="18874772"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63482"/>
    <dbReference type="eggNOG" id="KOG4558">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058669_1_0_1"/>
    <dbReference type="InParanoid" id="G3AV59"/>
    <dbReference type="OMA" id="IHQMAPW"/>
    <dbReference type="OrthoDB" id="5394411at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024624">
        <property type="entry name" value="Pyridox_Oxase_Alr4036_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28243">
        <property type="entry name" value="AGL049CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28243:SF1">
        <property type="entry name" value="PYRIDOXAMINE 5'-PHOSPHATE OXIDASE ALR4036 FAMILY FMN-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12766">
        <property type="entry name" value="Pyridox_oxase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase Alr4036 family FMN-binding" evidence="2">
        <location>
            <begin position="8"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW29862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="253" mass="29035" checksum="6A36F9B82BDF276F" modified="2011-11-16" version="1" fragment="single">MLQCHFLAPWVSTFNSCIDNELSSDKNNTPFITFQLATVDNISGFPRNRTLVYRGWLFNNKSSNVITFATDKRMNKYQELLANDKFEAVFYFPHVRKQFRFRGRARLINDEYQPEIDLTAVQNGMHLMSHDNESIMTTNTNASIFEGKISQDDTTNTTTTTATNSPTSPIVCECKNNGTSTEIQKQPITSALLSPSIANDVYDDMSQISYIQPTKEEWNEEIKRQWSNLSKQMKKSYRKPTPKQPITDESAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVA1</accession>
    <name>G3AVA1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_63525</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Mitochondrion inner membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377670.1">
        <property type="nucleotide sequence ID" value="XM_007377608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVA1"/>
    <dbReference type="FunCoup" id="G3AVA1">
        <property type="interactions" value="928"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVA1"/>
    <dbReference type="GeneID" id="18874791"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63525"/>
    <dbReference type="eggNOG" id="KOG3479">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141397_3_0_1"/>
    <dbReference type="InParanoid" id="G3AVA1"/>
    <dbReference type="OMA" id="QDFLRMY"/>
    <dbReference type="OrthoDB" id="1551503at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042719">
        <property type="term" value="C:mitochondrial intermembrane space chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140318">
        <property type="term" value="F:protein transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.810:FF:000008">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit TIM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050673">
        <property type="entry name" value="Mito_inner_translocase_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13172">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="14">
        <location>
            <begin position="13"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW29904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="86" mass="10115" checksum="72DCB017B472DDC9" modified="2011-11-16" version="1">MDQLNVKEQQEFQQIVEQKQMKDFMNLYSGLVVRCFDDCVNDFTSASLSSKETSCIAKCSEKFLKHSERIGQRFQEQNEKLVQSQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVA7</accession>
    <name>G3AVA7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cerevisin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_63540</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377676.1">
        <property type="nucleotide sequence ID" value="XM_007377614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVA7"/>
    <dbReference type="FunCoup" id="G3AVA7">
        <property type="interactions" value="363"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVA7"/>
    <dbReference type="GeneID" id="18874793"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63540"/>
    <dbReference type="eggNOG" id="KOG1153">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011263_3_1_1"/>
    <dbReference type="InParanoid" id="G3AVA7"/>
    <dbReference type="OMA" id="RHPDVDY"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04077">
        <property type="entry name" value="Peptidases_S8_PCSK9_ProteinaseK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000007">
        <property type="entry name" value="Subtilisin-like serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000011">
        <property type="entry name" value="Vacuolar protease B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034193">
        <property type="entry name" value="PCSK9_ProteinaseK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF11">
        <property type="entry name" value="CEREVISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442645" description="Cerevisin" evidence="7">
        <location>
            <begin position="20"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="9">
        <location>
            <begin position="84"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="8">
        <location>
            <begin position="214"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW29910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="493" mass="52406" checksum="DC289A2959FD5B6D" modified="2011-11-16" version="1">MRLSRAVAISILTSLGVQGLVLPSIGDVIDVFGGVEKISDKINEKLEPLKEAAHDAAQHLGMQLSDALAPIVNIDSTRDIIPHRYIIVLNDDVKPEEASFHQEWVALTHANSLNNVEKDHPFFASTQDFKTEGGIIDSFRIDSLLNGYYGYFLDETIELIRKNPLVKFVERDSLVHANEFQADKGAPWGLARVSHRQPLSLSSFDQYLYDNEGGKGVTAYVIDTGVHVNHVEFGGRATWGSTIPTGDSDTDGNGHGTHCAGTIGSNAYGVAKKADIVAVKVLRSNGSGSMSDVVKGVEFAATSHQNAVKQSKKGFKGSTANMSLGGGKSPALDLAVNAAVKAGLHFAVAAGNENQDACNTSPASAENAITVGASTISDSRAYFSNYGKCVDIFAPGLNILSTYIGSDSSTAYLSGTSMASPHICGLLTYYLSLQPDSDSEFFVAADSVSPNQLKKNLIAYASTGVLSDIPEDTPNILAFNGAGQNLTDFWHGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVC1</accession>
    <name>G3AVC1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_68961</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377906.1">
        <property type="nucleotide sequence ID" value="XM_007377844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVC1"/>
    <dbReference type="GeneID" id="18875250"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68961"/>
    <dbReference type="HOGENOM" id="CLU_618434_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVC1"/>
    <dbReference type="OrthoDB" id="10327475at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="3"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="443" mass="51798" checksum="AF24553798C160E5" modified="2011-11-16" version="1">MISVTLSDLPHEVIQKIIDLLPRDILLELLHFKNNWAISHAFCAYYQKISIQSRIPNQSSNSYSNDIVNPFELNFGEYAVLGSRQSLINFLNSYPNFIPDEISFDNLDDLIAMHQTHDTLLTRVKRVRICEEKYDGEHSPHWFKMKQVQRYPYNIYSQQYKHDTVLCQCDDVQFQPSIQHLEIPQAKVMDSAVFFARLKNLRSFSSNELLHYNIEYLPKRLNYVSLLSSAILHLPTYDLELPPQVKQLQVRSLLPLDTYLNIRPSGSLTEIEFKGGKFKDMGYFRFPEHLTTLVFRDCEIDSFLDFNTSNKFSCLTALHISGYFGKLFYYYFFNTALPQSLTDLSFRNCKPSFQRGSTNDTVVYRYPESFTDFGFFKLNDSFELPKNLKKLYLKCPGVMIEPSWKLPKSLQSVELLDFTGTFKMPDLAMIQDYKIGHIKLEFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVR7</accession>
    <name>G3AVR7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_63849</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377998.1">
        <property type="nucleotide sequence ID" value="XM_007377936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVR7"/>
    <dbReference type="STRING" id="619300.G3AVR7"/>
    <dbReference type="GeneID" id="18874942"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63849"/>
    <dbReference type="eggNOG" id="KOG2710">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_761992_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVR7"/>
    <dbReference type="OrthoDB" id="3196451at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051025">
        <property type="entry name" value="RhoGAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228:SF25">
        <property type="entry name" value="F-BAR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228">
        <property type="entry name" value="SPERMATHECAL PHYSIOLOGY VARIANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="170"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="20"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="281"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="55"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="106"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="364" mass="40478" checksum="D46D0CC39BF4A646" modified="2011-11-16" version="1" fragment="single">MSHSTSPTTRNSIFGWAKGLKRGGSHFVSNDSVNEISSDESISRSPTKGITIQHSSSQPNNYTSSQQNTSSSQPQQQQTNIPNFGSSPTQQASDFLRPLLNHKSRSNNNINRVRSNSLNQSENSLRQHRDSFLQHNSLVDENSKYFGVPLHQALEEASSNISILAGDQSKELQYGQIPIVVAKCGVYLKKHGLAVEGIFRVGGSSKRLKELQVIFNTPPSYGKGLVWDGYTVHDAASILRRYLNALPEPLIPLEMYESFRDPLRSRPRIINYMKYKAENPSKSISNLESTNSSEVLDTSQPLTEANIGRLNCSDSNGDFKDSEDVPINPVFSPTGQSEQQEKLPSTEDDNAEKSKRKSKSYKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE22</accession>
    <name>G3AE22_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">biotin synthase</fullName>
            <ecNumber evidence="3">2.8.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_69759</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="13">Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="13">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the radical SAM superfamily. Biotin synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.6" evidence="3"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372967.1">
        <property type="nucleotide sequence ID" value="XM_007372905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE22"/>
    <dbReference type="FunCoup" id="G3AE22">
        <property type="interactions" value="220"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AE22"/>
    <dbReference type="GeneID" id="18875288"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_69759"/>
    <dbReference type="eggNOG" id="KOG2900">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033172_1_2_1"/>
    <dbReference type="InParanoid" id="G3AE22"/>
    <dbReference type="OMA" id="NICTTHT"/>
    <dbReference type="OrthoDB" id="2414104at2759"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00162"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004076">
        <property type="term" value="F:biotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000011">
        <property type="entry name" value="Biotin synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01694">
        <property type="entry name" value="BioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002684">
        <property type="entry name" value="Biotin_synth/BioAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024177">
        <property type="entry name" value="Biotin_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00433">
        <property type="entry name" value="bioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976">
        <property type="entry name" value="BIOTIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976:SF2">
        <property type="entry name" value="BIOTIN SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06968">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001619">
        <property type="entry name" value="Biotin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01060">
        <property type="entry name" value="BATS_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00272">
        <property type="entry name" value="biotin_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01278">
        <property type="entry name" value="biotin_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4 13" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11 13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="14">
        <location>
            <begin position="51"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001619-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW35555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="345" mass="38784" checksum="F9F6C549B5AF41BF" modified="2011-11-16" version="1">MGKVILSIIHFVNYQIRSTWTKTQIQEIYNTPLLELIHQSSLQHRQYHDPSEVQLCTLLSIKTGGCTEDCSYCAQSSRHNTGLQAEKLISVESVLEKARIAKANGSTRFCMGAAWRDMHGRKSGLRKIGDMVEQINAMGMETCVTLGMISEEQAVALKEKGLTAYNHNVDTSREHYPNVITTRTYDDRLDTIKNVQKAGISACTGGILGLGETPDDHVSFLYTLATLDPHPESLPINRLVPIIGTPMEKELRSLPQEKKLQFDQILRTIATARLIMPDSIIRLAAGRYTMKEHEQFLCFLCGCNAIFTGEKMLTTMCNGWDEDIAMLKKWGLRPMKSFRKELAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE79</accession>
    <name>G3AE79_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58831</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373025.1">
        <property type="nucleotide sequence ID" value="XM_007372963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE79"/>
    <dbReference type="GeneID" id="18872631"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58831"/>
    <dbReference type="HOGENOM" id="CLU_2265400_0_0_1"/>
    <dbReference type="InParanoid" id="G3AE79"/>
    <dbReference type="OrthoDB" id="10383259at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="103" mass="11818" checksum="F751ABFCA47DD847" modified="2011-11-16" version="1">MMNVKVFSTTADIESERDLIELIKGINSLESISNYNIIEIKDDDGKVYWSIYAESGKLNYISLVSSATRLLQAKESGCKVYRSKVCCSFDIDYFNPYELYKKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE81</accession>
    <name>G3AE81_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Golgi apyrase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_69800</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373027.1">
        <property type="nucleotide sequence ID" value="XM_007372965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE81"/>
    <dbReference type="FunCoup" id="G3AE81">
        <property type="interactions" value="257"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AE81"/>
    <dbReference type="GeneID" id="18875291"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_69800"/>
    <dbReference type="eggNOG" id="KOG1386">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010246_3_3_1"/>
    <dbReference type="InParanoid" id="G3AE81"/>
    <dbReference type="OMA" id="TAYEVCF"/>
    <dbReference type="OrthoDB" id="6372431at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004382">
        <property type="term" value="F:GDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045134">
        <property type="term" value="F:UDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046036">
        <property type="term" value="P:CTP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006256">
        <property type="term" value="P:UDP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24039">
        <property type="entry name" value="ASKHA_NBD_YND1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF121">
        <property type="entry name" value="NUCLEOSIDE-DIPHOSPHATASE MIG-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01238">
        <property type="entry name" value="GDA1_CD39_NTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="513"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="586"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="617"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="589"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="654"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="192"/>
            <end position="196"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW35615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="670" mass="75420" checksum="51901AA00D168C3E" modified="2011-11-16" version="1">MAEERAIALNDKNDKYGIVIDSGSSGSRIQIYKWSDPKTLLKSKSQSVLQSPPKIIQEPNWTKKISPGISTYNTNSKIKEIWSKHYSELMKFAEGIIPADKHGETPVFVLSTAGMRLLPKTTQNKILKETCSSIKKNTNFYLPDCSNFVQIIDGSTEGIYGWLGLNYLMGQFNNYDETLKSHQSIGFMDMGGASTQIAFVPSSPEQIKKHAEDLAKVTLRNVNGQTQSWDVFVATWLGFGANEARKRFLNQLINLSIVNEQTSNEINDPCLPKGATAEYGLDGKRYKIVGVGNYEMCTRTIYPLLMKAIPCQDEPCLFNGIHGPKLNFEKDKFIGISEYWYTANDIFQSGGEYNFKIFNQKVKEYCESDWGQILKNSEAGQYSNLDPDKFLKDACFKANWVMNILHDGFDLPRLGFEEEVKRATVKDHDITKVPVPFKSANSIEGEELSWTLGKILLFATSQIPPADSKDSLEIGIYPSEISRKSFVPGGISSKSSSSDYDSDDDDESGYGSLIYSIIFVLLLLFFLYHFGRSHFGKWGYKLRRLHMPISVRKSINSISSKIPYIGDYLRNWTSYEEIEQINLEEGLGDSSQKPVKPQSEANQHASILRTRSALALNEESEQDQNNTRVPNFMNKPFITKNGNGLFSESRESLPRLSSSSSLPRAKSAMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEC5</accession>
    <name>G3AEC5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 62</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58911</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373125.1">
        <property type="nucleotide sequence ID" value="XM_007373063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEC5"/>
    <dbReference type="FunCoup" id="G3AEC5">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="GeneID" id="18872676"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58911"/>
    <dbReference type="eggNOG" id="ENOG502RJPB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024079_2_0_1"/>
    <dbReference type="InParanoid" id="G3AEC5"/>
    <dbReference type="OMA" id="LFGQAKF"/>
    <dbReference type="OrthoDB" id="188042at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009291">
        <property type="entry name" value="Vps62"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053102">
        <property type="entry name" value="VPS_Associated"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48220:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 62-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06101">
        <property type="entry name" value="Vps62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003441977" description="Vacuolar protein sorting-associated protein 62" evidence="1">
        <location>
            <begin position="20"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="438" mass="50525" checksum="891A0726F7F99A7C" modified="2011-11-16" version="1">MLYPIFWLITLGTCIPLSSFNVQETTNYILNAINDPQKEIQIENILKNPLVYKDYPPIVSLPKKDQRTLKDGEIPDYVLKYSPLVHLYSEERYLPYDIQKFVTNFHVTYKNGTIIPGTEEDMTLEKLSKLPKHSEIFLTSNSDFDKNPEWITGFKNKPNLINGEIKDAPAVLITVDKGNGWVDSYWFYFYSFNLGPFVMGQGPYGNHVGDWEHSLVRFYKGEPIIVWMSAHGGGGAFYYKNLEKYSLDEKHPIIFSARGTHANYPSVGQYPHDLPYGILSDFTDRGPLWNPSKNYLGYTYDGKHVFPTTNHSNPKQAGREWQYGNWLSYTGHWGDQQLPDDDPRQVYSFIGGYKYIDGPMGPLMKNLLRLKPCERAKWWNFWSGCNVRQNIKWGVGIESEGYNCGNLFINVRPTWLKSGLQRVTWGGGFCYIIDLIYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEL0</accession>
    <name>G3AEL0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Meiotically up-regulated protein Msb1/Mug8 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_47855</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372184.1">
        <property type="nucleotide sequence ID" value="XM_007372122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEL0"/>
    <dbReference type="STRING" id="619300.G3AEL0"/>
    <dbReference type="GeneID" id="18871152"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_47855"/>
    <dbReference type="eggNOG" id="ENOG502QUEY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006614_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEL0"/>
    <dbReference type="OMA" id="ITEANWI"/>
    <dbReference type="OrthoDB" id="3362494at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04401">
        <property type="entry name" value="RhoGAP_fMSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22541">
        <property type="entry name" value="SP5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037508">
        <property type="entry name" value="Msb1/Mug8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012965">
        <property type="entry name" value="Msb1/Mug8_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28093">
        <property type="entry name" value="MORPHOGENESIS-RELATED PROTEIN MSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28093:SF1">
        <property type="entry name" value="MORPHOGENESIS-RELATED PROTEIN MSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08101">
        <property type="entry name" value="Msb1-Mug8_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Meiotically up-regulated protein Msb1/Mug8" evidence="2">
        <location>
            <begin position="40"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="451"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="532"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="633"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="705"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="829"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="646"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="656"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="745"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="766"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="792"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="878"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="919"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1011"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1066"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1096"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1118"/>
            <end position="1129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1180" mass="133287" checksum="068CBDF6D25B9BA9" modified="2011-11-16" version="1">MATKQLPHIPDLELPKLPEVKKSKDSRSTSVDSGIIPYSQFTRNNLKSIIHSLTQELKKRGTKTPYIFLPFRSKINDSHLEKLLSFVFPNGEIIDAAKAEAGLLEFDEFTLICGLKYLWARLPNNEIIGWDVYLEFKRKEKEKGYPKNAFLAIMPKCLSSPAHASIVYDFLDLIVNITSNSHDNFLSGRKISKMASFWAFNPMHSKKPQEFDFIEGIEMWTKSTNALFHLVLSFLRSMLPETDMETLKLPKSLQSLLVTNQYPPVEERGEALKNMITIPCVHIKSTRPSKNAYDLISKVRYSLSFDKRDSFLSVENYTILKKLFNKKGTSEIVETLTEESRRILARITAEPAESQYNLEPGWAKSPVPFDEDIPQYSKIEIKNVSIQDYYIWTWLSSLGSDQTSHMKRLFGRSIVVEADLLGFQKWIVISEKTMSSEEYLRNFNLDHNSDYKSELKDKNLPPVPDKNKGSRESSRPSSEETDKEFSDEFSYIYNSSLCTDDNDSSIEDIHQSSKELNTTSKKLSKLMISEALQKKPQIRKAPPPPTVESRKTPPMLNEYSLKKNITKSPESVGSSFIEPFEQYHTSMEKRHKLKEDILHSEPFDNYHVGRGIPEVEQHKQVDAVVQADEAEKVRKLKQQQEEEEAEKARKLEKQAQKAQLAAAQAAGFPFAFLPSNEPPPPPEQAAETARVVSDLMMTTPFLITIPGSENVLASPNKNKSKSRSPDRGVERGSSASPTRHAASKSPDRIRVSPEQRARSAQTSPERQVDIKPEPVVHPEAKEKKTLLGRKTPKYDKYEKELPPIVAPTPVTPVLQSNSITREAGITNLSGFVKSPLDPFESPKVVQEPELSAAHQYSLEQPQQHSSKHTSCHSPEHSLLQQHSPTHQTSPQRRSPEHSFTQQGLSPEQQIPERHPDHSLPQQRYSPQHPASQQRHSPEHLMPQQRQSPEYMPQEYSLPQQAPPHSEHSIPQSQQAQPHPDPRAIRKLAKSPMLQPAQPGFMGPPPRLGSPQLASSPRQRSDGFQQPRPLRKQQSIPNIAPPRIVTGYGSPQLPPSPGYFPQQQYGPPSPGYFPQQYPPQQHPQYAPPQQYPPQQYPPQQGYPPQQGYPPHGYPPQGYPPQGYAPQHGYGPAPGHGNMRGAPRGGKYNDMMGVPVVGKHNKNITENKASLRAAFVQGNFGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AES2</accession>
    <name>G3AES2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">2-hydroxyacid dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_48666</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373110.1">
        <property type="nucleotide sequence ID" value="XM_007373048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AES2"/>
    <dbReference type="FunCoup" id="G3AES2">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AES2"/>
    <dbReference type="GeneID" id="18871252"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48666"/>
    <dbReference type="eggNOG" id="KOG0069">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_2_1"/>
    <dbReference type="InParanoid" id="G3AES2"/>
    <dbReference type="OMA" id="LPWCMGA"/>
    <dbReference type="OrthoDB" id="298012at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF178">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE YGL185C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="4">
        <location>
            <begin position="67"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="5">
        <location>
            <begin position="182"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="39862" checksum="7B01F317C16DC9E1" modified="2011-11-16" version="1">MAKPQVLFIGELNRTLPEFIDFQSKYDCIFYTLTTREEFISKLETDFKNIVAIYAAWLGFLPIGGFRSVIDHSPANLKIIALCSVGYDHEDADVLAKHGITMTNVPSDGAAEPVAELVLVNAITSFRQFHLYSQFRNVKNTLKIRYQHSAGEFDKTTGKPTSLSDSNGYYFGHYINNRGCLSPRGHNVAIVGFGNIGQCIGKKLSDAGMNVTYIKRNKLSSEQENKLGYKVTYKAKIEDATDADLVVIACPATPETFHLINKDVIDSMQKPFRIVNIGRGTSIDEQALVDGLKSGKVLFAGLDVFEEEPKVHEELLNREDVVLTPHIGAATVENFDYTATRSLQNIDDVINGGNGLSRVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEV3</accession>
    <name>G3AEV3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Methionine aminopeptidase</fullName>
            <ecNumber evidence="11">3.4.11.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_58988</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 11">Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="11">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="9">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with zinc, cobalt, manganese or divalent iron ions. Has high activity with zinc; zinc cofactor is transferred into the active site region by the ZNG1 zinc chaperone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Associates with the 60S ribosomal subunit of the 80S translational complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="11"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373195.1">
        <property type="nucleotide sequence ID" value="XM_007373133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEV3"/>
    <dbReference type="FunCoup" id="G3AEV3">
        <property type="interactions" value="879"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEV3"/>
    <dbReference type="MEROPS" id="M24.017"/>
    <dbReference type="GeneID" id="18872710"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58988"/>
    <dbReference type="eggNOG" id="KOG2738">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015857_2_1_1"/>
    <dbReference type="InParanoid" id="G3AEV3"/>
    <dbReference type="OMA" id="FYGDHAY"/>
    <dbReference type="OrthoDB" id="3209743at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01086">
        <property type="entry name" value="MetAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000010">
        <property type="entry name" value="Methionine aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01974">
        <property type="entry name" value="MetAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002467">
        <property type="entry name" value="Pept_M24A_MAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031615">
        <property type="entry name" value="Zfn-C6H2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00500">
        <property type="entry name" value="met_pdase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43330">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43330:SF7">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15801">
        <property type="entry name" value="zf-C6H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00680">
        <property type="entry name" value="MAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52013">
        <property type="entry name" value="ZF_C6H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9 11" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9 11" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C6H2-type" evidence="12">
        <location>
            <begin position="2"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>a protein</name>
            <dbReference type="ChEBI" id="CHEBI:16541"/>
        </ligand>
        <ligandPart>
            <name>N-terminal L-methionine residue</name>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>4</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>4</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>a protein</name>
            <dbReference type="ChEBI" id="CHEBI:16541"/>
        </ligand>
        <ligandPart>
            <name>N-terminal L-methionine residue</name>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>4</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>4</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW35783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="370" mass="41019" checksum="5EDDF0F9706FB1F6" modified="2011-11-16" version="1">MSAICASPNCGKETESSLKCPVCLKAGVSCVFCNQNCFRSSWTTHKAIHSAEDGAEEYNPFPNFNFTGELRPTYPLSPRRAIPKHIKLPDYAQAGKPISEIRDDRIGRIPVLTPKEITKIRKCAKVSREILDITASHIKPGVTTDELDAILHRECVKRNAYPSPLNYYNFPKSCCTSVNEVICHGIPDKTKLQDGDIVNLDVTIYYLGFHSDLNETYYVGDKAKCNPDIVRLVETTRESLDLAIQAVKPGIAFRDLGAIIEKHAHENNCSVVRTYCGHGCGALFHCQPNVPHYAKNKAVGIAKPGMVFTIEPMLNLGTFKDLQWPDNWTAVTQDGKFSAQFEHMLLVTEDGVEVLTARTETSPGGPIPRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEZ9</accession>
    <name>G3AEZ9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_57874</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372215.1">
        <property type="nucleotide sequence ID" value="XM_007372153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEZ9"/>
    <dbReference type="GeneID" id="18872233"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57874"/>
    <dbReference type="HOGENOM" id="CLU_2159998_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEZ9"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="111" mass="12584" checksum="13AFFFF7F43CA162" modified="2011-11-16" version="1">MFRIAQVSRGSVQLSRRILASNGSASPVFKRYNSQSQIKHQLDNTKAYLKKVKVEDSKESEQVLAYFRKAGVVTAGGILLFYAYAITVQMLNKEDEEPEVVPVLSQDVRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF29</accession>
    <name>G3AF29_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">UDP-glucose 4-epimerase</fullName>
            <ecNumber evidence="12">5.1.3.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GAL10</name>
        <name evidence="12" type="ORF">SPAPADRAFT_57928</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Mutarotase converts alpha-aldose to the beta-anomer. It is active on D-glucose, L-arabinose, D-xylose, D-galactose, maltose and lactose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>UDP-alpha-D-glucose = UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:22168"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="5.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">In the C-terminal section; belongs to the aldose epimerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the N-terminal section; belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.2" evidence="12"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372245.1">
        <property type="nucleotide sequence ID" value="XM_007372183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF29"/>
    <dbReference type="FunCoup" id="G3AF29">
        <property type="interactions" value="515"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AF29"/>
    <dbReference type="GeneID" id="18872251"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57928"/>
    <dbReference type="eggNOG" id="KOG1371">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_22_1_1"/>
    <dbReference type="InParanoid" id="G3AF29"/>
    <dbReference type="OMA" id="KGLYREW"/>
    <dbReference type="OrthoDB" id="9402762at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052574">
        <property type="term" value="P:UDP-galactose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05247">
        <property type="entry name" value="UDP_G4E_1_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018052">
        <property type="entry name" value="Ald1_epimerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005886">
        <property type="entry name" value="UDP_G4E"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01179">
        <property type="entry name" value="galE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007956">
        <property type="entry name" value="PRK10675.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43725">
        <property type="entry name" value="UDP-GLUCOSE 4-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43725:SF47">
        <property type="entry name" value="UDP-GLUCOSE 4-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00545">
        <property type="entry name" value="ALDOSE_1_EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="7 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="11">
        <location>
            <begin position="5"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW34833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="685" mass="76221" checksum="1E51B5F907231924" modified="2011-11-16" version="1">MSEYILVTGGAGYIGSHTVIELINNGYKVVIVDNLSNSSYDAVARIEFIVKQHVPFFNVDLKNYEELNKVFQQYKISGVIHFAALKAVGESTKIPLDYYENNVSGTVTLVKACKANNVHNLVFSSSATVYGDVTRFGDNSMIPIPEHCPMDPTNPYGRTKSVIENILKDVYNSEESAWKFAILRYFNPIGAHPSGLLGEDPLGVPNNLLPYLAQVAIGRRDKLSVFGNDYDSHDGTPIRDYIHVVDLAKGHIAALDYLKNIEQGKGLYREWNLGTGKGSTVFDVYHAFVKAVGKDLPYEIVGRRAGDVLDLTANPARANKELKWEAKLSIEQACKDLWKWTTENPFGFNVENYSWTTFESDGNYDNRLHTFTNGPLTVKLANRGALIQDIVFNGERLVKAFDNGDDFKLPTNPYFGTVVGRFANRIADGKFELDGKSYQLTLNEGPNNLHAGFNGFDKNNLLGPVVKFNDGKFTVEFLIVDQDGNDGFPGTLETVVKYVIDNKSLEAEYEAHLVEGEATIVNMTNHSYFNLTDEANTNGTEVKLVSDIMMEVDAKSLLPTGKFIKNEKVTPNKPITLDDDAFFDYCFIRDEAGCGIDTRSLPVVPVFSAEHPKTTTRLTVLTTEPAFQFYTGDGVNTKGFGKRAGFCLEPSRFVNAPNHKEWAGQVTLRKGEVYGTIIKYAFEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AF48</accession>
    <name>G3AF48_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Alpha-1,3-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_69954</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373236.1">
        <property type="nucleotide sequence ID" value="XM_007373174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AF48"/>
    <dbReference type="FunCoup" id="G3AF48">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="GeneID" id="18875299"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_69954"/>
    <dbReference type="eggNOG" id="ENOG502RZ48">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015387_0_1_1"/>
    <dbReference type="InParanoid" id="G3AF48"/>
    <dbReference type="OMA" id="MYCAFIG"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000033">
        <property type="term" value="F:alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392:SF1">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW35824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="635" mass="74071" checksum="AD44CF2FF9ACDA12" modified="2011-11-16" version="1">MIYSLIRIFLGLIFPISTVGSSLVVVSQLKQELVDKRSSVYDIMLQNHNIDSILSLDFNQRCDLYFKTLYSIDPNYSFDANDDFAPQFNSPDFARFKREMGANKNEDTIRREYKKANFVRHQPKIRDTISILRVFNKCYIGPKDQHFQNQKPLTENNEALLKDNSIDLFKGSHTFEKRIYPWLSGEAPIYEDWTGKTYFDVPGAPEPISLNPYWFNRFQEKFNGRGIVLTIGEKHIENTERLIRVLRALKNKYPIQILVDEDFSLESKQRILAAARDDFTQLPFSYNKVASILGNNYWTPGEGLPRQDIWFVNIKNSIVPAYWPRFNGFTKKLLANIFNSFEEFMLIDCDSVILESPEFFFKIKEYQDTGAYFFKDRSSEFRGKDSINFFKSLGPSIIDNLMFDIPMMTSHTLNREFFKGLGHYMESGLVLFNKKQHFRSLLMMLQLSFFEPVNREVYGEKEYYWLGMAFDGDESYEFEPNFAAAIGNFSKEDEPTKEICSSHPAHLSAHDGHLLWINSGFRFCERAKMVDYNKEASIGARFSNDPVELRSIFESLLRPSHGIIPPIPPTFTYMRNRDNQPSRGWVPDRRYCRSKLYCAYSRIGGDDNPEFGQIITFNDTAKDIMAYYGDVWLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFB7</accession>
    <name>G3AFB7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Multidrug resistance protein CDR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_131428</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372318.1">
        <property type="nucleotide sequence ID" value="XM_007372256.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AFB7">
        <property type="interactions" value="399"/>
    </dbReference>
    <dbReference type="GeneID" id="18869575"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131428"/>
    <dbReference type="eggNOG" id="KOG0065">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_35_0_1"/>
    <dbReference type="InParanoid" id="G3AFB7"/>
    <dbReference type="OMA" id="PEVEICF"/>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005285">
        <property type="entry name" value="Drug-R_PDR/CDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00956">
        <property type="entry name" value="3a01205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="501"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="535"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="577"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="613"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="643"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="749"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1258"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1304"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1338"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1454"/>
            <end position="1475"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="139"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="845"/>
            <end position="1089"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1500" mass="170504" checksum="605E19CB207A94C1" modified="2011-11-16" version="1">MFKEDYLTNSGSFEANPHESYLGFNEEASDDVRQLARKITQDYATSDQSSSVPYLSSNDVVPAINPYQDELTGSNLDPESPKFDSKYWIKNLKALYQSDPDYFKPNKLGVAFRDLRAYGIANDFDYQTTVATGLIKFVVDGSRYFRKDDKSRYFDILKPMDGLLRPGELTVVLGRPGAGCSTLLKTLAVNTYGFQVDKDSKVSYDGLTPDEIEKHYRGDVIYSAETDEHFPQLTVGDTLEFAARLRTPQNRGLGVDRETYAKHMASVYMATYGILRQRNTPVGNEFVPGCSGGERKRVSIAEVSLSGANIQCWDNATRGLDAATALEFIRALKTSSAVLDVTPVIAIYQCSQDAYDLFDNVSVLYGGHQIFFGKANKAKEYFTNMGWYCPERQTTADFLTSLTNPEERVAAPGYEGKLPRTAQEFESYWKKSSEYLSLIADIDEYFVECEKLNTKQAYHDSHVARQSNHISPRSPYTVSFPMQVKYIMGRNFLRTKNDPSIAIFTVVGQGMMGLILSSIFYNLDQTTNYMNYRGIALFFGVLYNAFASLMEILSLFEARNIIEKHRKYALYRPSADAIASVIFELPIKFMMSITFNLIFYFMINLRREPGRFFYYWLMAIWCTLIMSHLFRCIGSLVTTVAQAMTPATVMLLAMVMYTGFVITTKEMLGWARWINYINPVAYVFESFMVNEFHGRYFECSNFVPSGPSYDNITFINRVCTAIGAQPGESLVSGTTYLKMAYNYDNTHKWRNLGICIGYALFFLVVYIMLTEFNKGAMKKGEITLFLRSSLKQLKKRKELAAQKQDIESASNEKVPLAEDLANSDFEKKEIVELSENGLTGENETFLWRDLTYEIRIKKEDRIILDHVDGWVKPGEITALMGATGAGKTTLLNCLSDRHTVGVITDGVKMVNGHSLDSSFQRSIGYVQQQDLHLQTSTVREALKFSAYLRQSNTTPDKEKDAYVDYIIDLLEMSEYADAMVGVAGSGLNIEQRKRLTIGVELVAKPKLLLFLDEPTSGLDSQTAWSICKLMRKLADHGQAILCTIHQPSALLLTEFDRLLFLQEGGQTVYFGDLGENCQTLINYFEKYGADACPKDANPAEWMLKVVGAAPGSHAEADYFQVWRKSEEYNEIQSELDKMEVELALLPKNNDPEAHLKYAAPWWKQYVIVTARTLLQNWRTPSYIYSKLFLCLGTSLFNGFSFFGGHLSLRGLQNQMFAIFLFYVPFNTMHEQMLPYFLRQRAVYEIRERPSRTFSWFAFISAQLTSEIPYQIVVGTLSFFCWYYPSGLYKNASPTNEEESRGALMWLLVVSFYCYISTMGHFCISFVEREENGANMGHFLFQMCLMFCGVLAGPAQLPGFWIFMYRCNPITYLTQAMMAVGLANNHVVCAPQEYVSINPPDGISCEQFMEDYISKAGGYILADEATGQCQFCQMNNTNEFLASVGSFYSQRWRNYGIFVAFIAANVLFSIFLYWLGRVPKGTRERKKVTKDESKVGRRWLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFE3</accession>
    <name>G3AFE3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial</fullName>
            <ecNumber evidence="11">1.14.15.45</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">2-methoxy-6-polyprenolphenol 4-hydroxylase</fullName>
            <ecNumber evidence="11">1.14.15.46</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">COQ6</name>
        <name evidence="13" type="ORF">SPAPADRAFT_58061</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">FAD-dependent monooxygenase required for two non-consecutive steps during ubiquinone biosynthesis. Required for the C5-ring hydroxylation during ubiquinone biosynthesis by catalyzing the hydroxylation of 4-hydroxy-3-(all-trans-polyprenyl)benzoic acid to 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoic acid. Also acts downstream of coq4, for the C1-hydroxylation during ubiquinone biosynthesis by catalyzing the hydroxylation of 2-methoxy-6-(all-trans-polyprenyl)phenol to 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol. The electrons required for the hydroxylation reaction are funneled indirectly to coq6 from NADPH via a ferredoxin/ferredoxin reductase system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 2-methoxy-6-(all-trans-polyprenyl)phenol + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = a 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:81183"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10858"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="ChEBI" id="CHEBI:84166"/>
            <dbReference type="EC" id="1.14.15.46"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 4-hydroxy-3-(all-trans-polyprenyl)benzoate + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = a 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoate + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:81195"/>
            <dbReference type="Rhea" id="RHEA-COMP:9514"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10930"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:64694"/>
            <dbReference type="ChEBI" id="CHEBI:78396"/>
            <dbReference type="EC" id="1.14.15.45"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UbiH/COQ6 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.15.45" evidence="11"/>
    <dbReference type="EC" id="1.14.15.46" evidence="11"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372344.1">
        <property type="nucleotide sequence ID" value="XM_007372282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFE3"/>
    <dbReference type="FunCoup" id="G3AFE3">
        <property type="interactions" value="422"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFE3"/>
    <dbReference type="GeneID" id="18872300"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58061"/>
    <dbReference type="eggNOG" id="KOG3855">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_8_0_1"/>
    <dbReference type="InParanoid" id="G3AFE3"/>
    <dbReference type="OMA" id="VKQMQVW"/>
    <dbReference type="OrthoDB" id="683240at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120538">
        <property type="term" value="F:2-methoxy-6-polyprenolphenol 4-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106364">
        <property type="term" value="F:4-hydroxy-3-all-trans-polyprenylbenzoate oxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000021">
        <property type="entry name" value="Ubiquinone biosynthesis monooxygenase COQ6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000239">
        <property type="entry name" value="Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03193">
        <property type="entry name" value="COQ6_monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018168">
        <property type="entry name" value="Ubi_Hdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010971">
        <property type="entry name" value="UbiH/COQ6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051205">
        <property type="entry name" value="UbiH/COQ6_monooxygenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000689">
        <property type="entry name" value="UbQ_mOase_COQ6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01988">
        <property type="entry name" value="Ubi-OHases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43876">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS MONOOXYGENASE COQ6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43876:SF7">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS MONOOXYGENASE COQ6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01304">
        <property type="entry name" value="UBIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0274">FAD</keyword>
    <keyword evidence="3 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="FAD-binding" evidence="12">
        <location>
            <begin position="209"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="12">
        <location>
            <begin position="366"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="490" mass="54146" checksum="ADE781E6D1367802" modified="2011-11-16" version="1">MLSRSIRYFSNSAIRAATQVAPPTLQDVVIIGGGPAGLSLLATLKNSPKTKHLNCTLIEGGSLDKVREFATNPPQEYTNRCVSITPKTIDYMKHKIGSWDFIHEDRIKEYQGIIAYDGQDADSRVEFDVNSIGKDNLAAMCENINIQASLLAKVESLGLEDTTTIKDNTKVVDIINPLTPDLENHDLNEIDEVPGTGKLDWPIIKLSTGESIQTRLLVGCDGYNSPVRKYAQIESRGWQYNTFGVVATVKLQYEDFRSVGWQRFLTTGPLAILPLSEDNATIVWSSTPELSEILVKVNKDIFPRLVTAAMVLEETDLKYIYGLLKENPDDLSAVEEIEWRMSKVPEVVLEERYPAAIVSVLDGSRARFPLKLSHADTYVAPRVALVGDAAHTIHPLAGQGLNMGQSDVIHLVEALEKGIDRGLDLGSTLVLESYVSNAWPANHAMLGICDKMHKLFSTDFYPLVLARGLGMKTVNFLSPVKDMMMKSISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFK7</accession>
    <name>G3AFK7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Macro-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_53359</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372408.1">
        <property type="nucleotide sequence ID" value="XM_007372346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFK7"/>
    <dbReference type="FunCoup" id="G3AFK7">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFK7"/>
    <dbReference type="GeneID" id="18871777"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53359"/>
    <dbReference type="eggNOG" id="ENOG502SAAY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058081_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFK7"/>
    <dbReference type="OMA" id="YIIHCPT"/>
    <dbReference type="OrthoDB" id="6082470at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028071">
        <property type="entry name" value="Macro-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14519">
        <property type="entry name" value="Macro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro-like" evidence="1">
        <location>
            <begin position="56"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="285" mass="33562" checksum="6D4F3D0B3C36A1DA" modified="2011-11-16" version="1">MKIILIDSNPIIVKCWKLHYSHIVKYISRHNLQLIYPHDYQFHNLTIEDMIKSQTFTGTTSVVTPTNSLCYMGGGFDLYLLKGMLLGTNITSYKHIENILQHHQMTKHHGYLSPNTIYKIDLVKVFENQYDYKSSTIYQNWNLVEMAQLPTMVVPEMIHRMTHIFDGLWNLLHEYHGDATDIDNLVIPGIGTGYGHLDEYESTKLMIFTFFIYNLKLDSTRLDQLRKSCLILFFFNKDYHHLRNTSDIEEIENCIVTGYGKEKRKNLKEDTIMEFEELFKCVKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFQ1</accession>
    <name>G3AFQ1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58172</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372452.1">
        <property type="nucleotide sequence ID" value="XM_007372390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFQ1"/>
    <dbReference type="STRING" id="619300.G3AFQ1"/>
    <dbReference type="GeneID" id="18872356"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58172"/>
    <dbReference type="eggNOG" id="ENOG502QSCZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066478_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFQ1"/>
    <dbReference type="OrthoDB" id="4019734at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="348" mass="38914" checksum="091D7A731A4E4BC3" modified="2011-11-16" version="1">MFSSTRQALGNGSKRFVRFNSKKATDDDFFANIFKRVEAVTTKVEEMKKTSPRTHYKRFQQKTSEGSGENRPRFNKAPRFIKPVEGEEQPRPIKTGNAGQGNTVRPRFTNRSDSQPTPYVNGERPQFVRRDPNRPAFSRTQSDRPQTHEPRQGQPRRPASGQPSSFRTRNGAKGNRRQGDADRKDAPTLFPRIVSKEAQPHALAPTINAETFFYGKVPSSNATVSARIASIAKLQLIKSKYPFNLPKNVIELAPRKLPGETINPFILQNNWNLTVGGKSLAYTVKRAVKGKIDYVQTGKATGSDVQFTSAMINKNASLALKQKQVLFDAVNGTISPRSLLANASWNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFT5</accession>
    <name>G3AFT5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">GABA/polyamine transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_58211</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372486.1">
        <property type="nucleotide sequence ID" value="XM_007372424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFT5"/>
    <dbReference type="GeneID" id="18872367"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58211"/>
    <dbReference type="eggNOG" id="KOG1289">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004495_0_3_1"/>
    <dbReference type="InParanoid" id="G3AFT5"/>
    <dbReference type="OMA" id="AFFWTWP"/>
    <dbReference type="OrthoDB" id="4476201at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015101">
        <property type="term" value="F:organic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000046">
        <property type="entry name" value="Amino-acid permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF29">
        <property type="entry name" value="AMINO ACID TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="362"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="437"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="481"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="515"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="574" mass="62781" checksum="AE16AF7612B1F7E6" modified="2011-11-16" version="1">MKPSASNTDKIHSNPISPVISQVHVDDLGISSILSNKHVTLNIDHEVDKDEALILAMGYKQEFKREFSLWSVFAVSFSVLGLLPSIAACFDYQQLVIGMSPIPWIIAVIFVTSVALSLAEVASAFPVASGTPYAVSRLAPPKYQSFLTWITCWSNWMCQITASPSVNYSGACMMLALRSYNDPSYTPTNGEIYALTLGIQIVHGIISSLPTGWLAKFNSAGTTCNILFLFIVFVMILAGNNRQELNPDIEGLTKFNVNAISWSLYNQTEWPTGIAMLMSFLGVIWAMSGYDSPFHLSEECSNAAVAAPKAIVLTSTVGGLIGFLFMIAIAYTVVDIDLIAQDPQGLGQPFVTYLAQIMNGKLVIAATALTIISSFFMGCSCMLAASRVTFAYSRDGMFPGSRWWKQVLPLTQTPVNAVWINLFLGQLFLLLMFAGDVAIGAIFSVGGIAGFVSFTMPTLLKITYARDKFVPGPWSLGKWSSPIGFVSVAFVAVMIPILCFPYVRGADLNPEEMNWTALVFFGPLLLATIWFVVDAHKWYVGPKSNLDEYIEYSDQDELEGIDGKVEHVTSNEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFW2</accession>
    <name>G3AFW2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase III subunit RPC4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_58243</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372513.1">
        <property type="nucleotide sequence ID" value="XM_007372451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFW2"/>
    <dbReference type="FunCoup" id="G3AFW2">
        <property type="interactions" value="162"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFW2"/>
    <dbReference type="GeneID" id="18872382"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58243"/>
    <dbReference type="eggNOG" id="KOG3122">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056234_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFW2"/>
    <dbReference type="OMA" id="NNYAGTH"/>
    <dbReference type="OrthoDB" id="5836119at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007811">
        <property type="entry name" value="RPC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13408">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13408:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05132">
        <property type="entry name" value="RNA_pol_Rpc4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="167"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="78"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="391" mass="43539" checksum="D8DE0F82B060546F" modified="2011-11-16" version="1">MSNRLESLNSKKPAGSPAPKGGLKFKPKVVQRKSKEERDKDAPIIKQEQQARQPATRGRGSTRGRGRGGRNAYAGTHLVTSGPLSAGSVSIGNVNGSKSGLTSDSIYSTSMSPTPEFLQNLKLKDRPKSVTPGAESEEDEEDDPTRINMTQQYRFADEETVLFPVRPDRTEVEKDTTTITLKSEEPTRENTPAFNRESPVKSEPLEDKIEKIKVSKAKLQERINQTDDPTAYEENKLLADHQQILDLLTHKFDELSTDEVPDDKFILFQLPNHLPEYIDKQQDESSEVKQEQEQEPSQLAVNKTNLRGRIGNINIHQSGKISIDLGNGNKLTVAKGAPTNFLQELACIEYDSSVAENEDVEMLDEQGRKVMGKVIRLGTVDEKVIATPYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG02</accession>
    <name>G3AG02_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_133256</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372553.1">
        <property type="nucleotide sequence ID" value="XM_007372491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG02"/>
    <dbReference type="GeneID" id="18869732"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133256"/>
    <dbReference type="eggNOG" id="ENOG502QTZH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025894_2_0_1"/>
    <dbReference type="InParanoid" id="G3AG02"/>
    <dbReference type="OMA" id="CRVIFFA"/>
    <dbReference type="OrthoDB" id="10027823at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520:SF5">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="418"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="27"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="448" mass="49196" checksum="FD6545585C167070" modified="2011-11-16" version="1">MRYTIPKFINVLMDEVGIVTVSKAGSDVAIIIALRMIRLIGFGATSLILALYMKALNITEEYIGFFMTLTFIGDLVSSFLLAIIADQVGRKKMLILCSLMMALTGIVFSFVDNFYVLTVVAVLGILTPSGVEVGPFRTIEQSSIASLVPHRDRSDIYAWYTFLGMFCGAFGSFLAGALVHTVNETYGYSLVFSYKCVFMAYALLSLISFVLCIFLSSNLEPAKTEPISESDSENTQLIPEVPTKSKLSSFRLLPHLSPHTYLIVLKLSLLFGLDAFASSLTPTSWISFYLKNKFDIPTTYLGSVFFTTGIVSAFTSLFSTSLTKRWGAVVTMVATHLPASMLLTFVPFPSSFVMTMAILVIRASTQSMDVAPKHVFLATLVADADRTAVFGFVNVVKTLAQVIGPSIVGFMTQKGLQWITFVIAGCLKTTYDIGILITFLTYNHHSEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGL6</accession>
    <name>G3AGL6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58581</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372767.1">
        <property type="nucleotide sequence ID" value="XM_007372705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGL6"/>
    <dbReference type="FunCoup" id="G3AGL6">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGL6"/>
    <dbReference type="GeneID" id="18872513"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58581"/>
    <dbReference type="eggNOG" id="ENOG502QSAP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042679_1_0_1"/>
    <dbReference type="InParanoid" id="G3AGL6"/>
    <dbReference type="OMA" id="AINCWAS"/>
    <dbReference type="OrthoDB" id="2125396at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="309" mass="35275" checksum="0B8CFFFFE78F5501" modified="2011-11-16" version="1">MIYRHPKLKASNFFNFVETIGNNKALGDYIHSLDLSYIIQTGKNAYVAKLLKRTAKSLETFVAPQTSFGLGPLIALKNCLNLRILDLRLVSETLKLEELFHSIRNLDKLTHLSFPRSSIEIGSYESINWPPNLKFLRISGGLNDDFLYESKFPESITELEFSHCPVISDLGLRQILYRIGRNLRTLKVQYPMPGLQKNSLDQVFIYCPDLRVLEISVDYVSSSFFDEEYLQYLPYPRPLRTLYIQSSGMLGTSTRLDPLDLAMALNEGRLPNLKNIQCTAKLGWDPKSEAVQYIADTLDERNGGIYIGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGQ0</accession>
    <name>G3AGQ0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">[histone H3]-trimethyl-L-lysine(9) demethylase</fullName>
            <ecNumber evidence="2">1.14.11.66</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_64512</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(9)-[histone H3] + 2 2-oxoglutarate + 2 O2 = N(6)-methyl-L-lysyl(9)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:60200"/>
            <dbReference type="Rhea" id="RHEA-COMP:15538"/>
            <dbReference type="Rhea" id="RHEA-COMP:15542"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.66"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the JHDM3 histone demethylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.66" evidence="2"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372795.1">
        <property type="nucleotide sequence ID" value="XM_007372733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGQ0"/>
    <dbReference type="STRING" id="619300.G3AGQ0"/>
    <dbReference type="GeneID" id="18874980"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_64512"/>
    <dbReference type="eggNOG" id="KOG0958">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001442_1_2_1"/>
    <dbReference type="InParanoid" id="G3AGQ0"/>
    <dbReference type="OMA" id="IPIQRCV"/>
    <dbReference type="OrthoDB" id="9547406at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051864">
        <property type="term" value="F:histone H3K36 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140684">
        <property type="term" value="F:histone H3K9me2/H3K9me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd15571">
        <property type="entry name" value="ePHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF7">
        <property type="entry name" value="[HISTONE H3]-TRIMETHYL-L-LYSINE(9) DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13771">
        <property type="entry name" value="zf-HC5HC2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="8">
        <location>
            <begin position="22"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="9">
        <location>
            <begin position="195"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="10">
        <location>
            <begin position="433"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="119"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="378"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="134"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="383"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="400"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW35383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="634" mass="74018" checksum="B864F6D90071CB11" modified="2011-11-16" version="1">MQHQYSEDPNLIIKPSYYSNGVPVFTPTYNQFHNFYQFNKAINQYGMQSGIVKIIPPAHWKHQLEKSKCYNHDNLENNITIRNPIVQHINQVSPGVYQQENIEKQRKYSIFQWKQLADQQNFQPPRTSTRKRSRQDDKEIVEDSPRKLRHTHSDYNIDIHEFTDDRCAELERIYWKTLTYAEPMYGADMIGSIFPPNIKSWNVAHLPNILDLMDRKIPGVNDAYLYAGLWKATFAWHLEDQDLYSINYLHFGAPKQWYSIPQEYRDKFFNLMKEIFREEYNHCHEFLRHKTFLVSPQVLEKHGIKVNHIVHRQGEFIITYPFGYHAGFNYGYNLAESVNFALDDWFPLGKVSNKCECINDAVTINISQLWSKFHGVEPDAPVKQNQDNSHSNTHISNGHAQKRPIGRPRKNSVRNDIPSEAPEETPSETPSATRECYLCPNSYPQTSPLFDLLPTDANHKIHRICANVFSNQLQLSNNKISNLQSISKQQKQLKCNICHQPNACIQCSYKKCSRSFHPSCALFDGVQFDIPDVYCKYHRSRQSSKPEHVIGKLVQFSINGRYFYGRVFSDNISEETVNIQVLPGLYDIIEVSYNNVLYCNYNENMNYLKEMIVMDDVNADDISKTRSGRKIKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGR1</accession>
    <name>G3AGR1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dynamin GTPase</fullName>
            <ecNumber evidence="3">3.6.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">SPAPADRAFT_131066</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.5" evidence="3"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372806.1">
        <property type="nucleotide sequence ID" value="XM_007372744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGR1"/>
    <dbReference type="FunCoup" id="G3AGR1">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGR1"/>
    <dbReference type="GeneID" id="18869551"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131066"/>
    <dbReference type="eggNOG" id="KOG0446">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008640_0_1_1"/>
    <dbReference type="InParanoid" id="G3AGR1"/>
    <dbReference type="OMA" id="PLKMGYV"/>
    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030061">
        <property type="term" value="C:mitochondrial crista"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097002">
        <property type="term" value="C:mitochondrial inner boundary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901612">
        <property type="term" value="F:cardiolipin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140523">
        <property type="term" value="F:GTPase-dependent fusogenic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180020">
        <property type="term" value="F:membrane bending activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070300">
        <property type="term" value="F:phosphatidic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015886">
        <property type="term" value="P:heme transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097753">
        <property type="term" value="P:membrane bending"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097749">
        <property type="term" value="P:membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990627">
        <property type="term" value="P:mitochondrial inner membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990626">
        <property type="term" value="P:mitochondrial outer membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000741">
        <property type="entry name" value="Putative mitochondrial dynamin GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056495">
        <property type="entry name" value="LIS_MGM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF212">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24550">
        <property type="entry name" value="LIS_MGM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13 17" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="20">
        <location>
            <begin position="166"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="19">
        <location>
            <begin position="737"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="80"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EGW35394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="847" mass="95184" checksum="9E8D8316ACB0D131" modified="2011-11-16" version="1">MNSARFNSRLIVPTRKFVFAKILARAAKAPAYIGGSVLAGGSYVAYKAEQASSYTQEKLSQIKDFADGFFDKTGEFLKGMGRSDSGGSGGNGGGGSDGNGGNVTTLGAAAAVGLSAEDDEEEEEAELEEEEDDEEFENDETQNHMLNLTRQMIEIRNLLSSIDHDGIKLPSIVVIGSQSSGKSSVLESIVGQEFLPKGSNMVTRRPIELTLVNTPESAANVAEFPALKMYNLTDFEQVQKILFDLNMAVPESEAISNDPIQVTIKSPTVPDLSLVDLPGYIQIEAADQPTQLKNKIRDLCNRYLEAPNVILAISAADVDLANSSALRASRVADPRGERTIGVVTKLDLVDPEQARKILTNKKYPLRLGYVGVITKAPNAIKSSGTLFSRKNLTGHQAFVAQQNFEYGYLREHKDDFYQCNVGTRNLRRKLMKVLERTMAASLRPTHLAIQSELEETAYQFKVEFNDRPLTPEMYLANNIDMIKLGVKELSHNFSRPELRALLKNELDQKVLDLLAERYWNKPFDLKKNSNVELDLRELAKITNIDDDIYWHKKLDLTTGSLTKLGVGRLSTNLITNALFTEVENLVDNTQLRNHPMAKAAVKEAAKNVLGAKYYSTADQVENCIKPYKYEIELEDREWQTSKENAVNLLREEIKQCDEVYQNLKNQVGGRKLQQVVTYLEKLKQSQESDIDLTSNETLGFSPTLIQRGKDAIFLKDRAALLKMRYSIVKNSKMCKKKEGKYQCPEIFLDAVASKITSTAILFLNVELLSDFYYNFPRELDQRFFNTMTAEEIERFAKEDPKIKKHIELQQRKDLLENALSKIESVLAIQRTTEPQKDDKKSSIFPWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGW7</accession>
    <name>G3AGW7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Adenylate kinase isoenzyme 6 homolog</fullName>
            <shortName evidence="10">AK6</shortName>
            <ecNumber evidence="10">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Dual activity adenylate kinase/ATPase</fullName>
            <shortName evidence="10">AK/ATPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_57692</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has also ATPase activity. Involved in the late cytoplasmic maturation steps of the 40S ribosomal particles, specifically 18S rRNA maturation. While NMP activity is not required for ribosome maturation, ATPase activity is. Associates transiently with small ribosomal subunit protein uS11. ATP hydrolysis breaks the interaction with uS11. May temporarily remove uS11 from the ribosome to enable a conformational change of the ribosomal RNA that is needed for the final maturation step of the small ribosomal subunit. Its NMP activity may have a role in nuclear energy homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with small ribosomal subunit protein uS11. Not a structural component of 43S pre-ribosomes, but transiently interacts with them by binding to uS11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the adenylate kinase family. AK6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="10"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372052.1">
        <property type="nucleotide sequence ID" value="XM_007371990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGW7"/>
    <dbReference type="FunCoup" id="G3AGW7">
        <property type="interactions" value="858"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGW7"/>
    <dbReference type="GeneID" id="18872154"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57692"/>
    <dbReference type="eggNOG" id="KOG3347">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079096_3_0_1"/>
    <dbReference type="InParanoid" id="G3AGW7"/>
    <dbReference type="OMA" id="QCEIFGT"/>
    <dbReference type="OrthoDB" id="10251185at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000372">
        <property type="entry name" value="Adenylate kinase isoenzyme 6 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00039">
        <property type="entry name" value="Adenylate_kinase_AK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020618">
        <property type="entry name" value="Adenyl_kinase_AK6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595:SF0">
        <property type="entry name" value="ADENYLATE KINASE ISOENZYME 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595">
        <property type="entry name" value="POS9-ACTIVATING FACTOR FAP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 10" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="NMPbind" evidence="10">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="10">
        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="207"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="210"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW34640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="243" mass="27785" checksum="3808A6415917F00C" modified="2011-11-16" version="1">MTSTTNRAMSRRYFPNIIITGTPGCGKSSHSESLVSHLNQELSKKGQEFTHLNVSDLAKERGCIESYDERLDTSVVDEDKLLDSLEVDLEKGGAIVDWHCCDIFPERLIDLVVVLRTDNSNLYDRLKKRNYNEAKLQENLDCEIMEVVLNDAREAYIPEIVIELNSNSAEEMDDNVERIGQWVTNWIKDHPDGVTNELDPELAALAREEQGDDEEEDEDFEIAEEGAYDSDDDEEEYDDDDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH78</accession>
    <name>G3AH78_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_48494</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372921.1">
        <property type="nucleotide sequence ID" value="XM_007372859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH78"/>
    <dbReference type="FunCoup" id="G3AH78">
        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="GeneID" id="18871231"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48494"/>
    <dbReference type="eggNOG" id="ENOG502RQDE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147564_0_0_1"/>
    <dbReference type="InParanoid" id="G3AH78"/>
    <dbReference type="OMA" id="LWENKHS"/>
    <dbReference type="OrthoDB" id="416496at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="148" mass="17150" checksum="056729D7D521B6E7" modified="2011-11-16" version="1">MDNSIPLTLEEFKVRISKISEAELQDQKQEQRSLLLHLVETNNELDSEIHLLHRKEIKTNEDLNDIKFYKETVLENKGVILDKVARISAINDELVARGILDEKMKSDEEEKLISEVVQKDITNVCDKKEETIDVPETNEQESEEGVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHF6</accession>
    <name>G3AHF6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">60S ribosomal export protein NMD3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_59543</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as an adapter for the XPO1/CRM1-mediated export of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the NMD3 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373704.1">
        <property type="nucleotide sequence ID" value="XM_007373642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHF6"/>
    <dbReference type="FunCoup" id="G3AHF6">
        <property type="interactions" value="1386"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHF6"/>
    <dbReference type="GeneID" id="18872964"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59543"/>
    <dbReference type="eggNOG" id="KOG2613">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027444_2_0_1"/>
    <dbReference type="InParanoid" id="G3AHF6"/>
    <dbReference type="OMA" id="VILVRKH"/>
    <dbReference type="OrthoDB" id="203821at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039768">
        <property type="entry name" value="Nmd3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007064">
        <property type="entry name" value="Nmd3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048899">
        <property type="entry name" value="NMD_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048898">
        <property type="entry name" value="OB_NMD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746:SF2">
        <property type="entry name" value="60S RIBOSOMAL EXPORT PROTEIN NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04981">
        <property type="entry name" value="NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21193">
        <property type="entry name" value="NMD_SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21192">
        <property type="entry name" value="OB_NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nmd3 N-terminal" evidence="10">
        <location>
            <begin position="23"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="60S ribosomal export protein NMD3 SH3" evidence="12">
        <location>
            <begin position="255"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="60S ribosomal export protein NMD3 OB-fold" evidence="11">
        <location>
            <begin position="319"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="465"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="436"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="483"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="516" mass="58686" checksum="DFDCBFD4E55BDE43" modified="2011-11-16" version="1">MSNYTHMDPNHNHGAGPVATVLCCNCGVPMDGSQGLVMCYDCIKLTVDITEGIPREANVSFCRNCERFLQPPGQWIRAELESRELLALCLRRLKGLNKVRLVDASFIWTEPHSRRIRVKLTVQGEAMANTIVQQTFEVEYVVIAMQCPDCAKSYTTNTWRAAVQIRQKVPHKRTFLYLEQLILKHNAHVDTISIQESKDGLDFFYSQKNHAAKMLDFLSAVAPVKAKKSEELVSTDIHSGSSQYKFSFSVEIAPICRDDLVVLPKKLAHSLGNISRLVLCSKVTNSIQFIDPNTLQIADLSPSIYYRSPFPSLLDATQLVEFIVLDVEPTGDIRGKHVLADIEVSRAADMGVNDQSFYVRTHLGGILHPGDSCMGYYLTNTNFNSDLWDTLDSDNIPEVVIVKKHYARKNKKNKYRKWKLKRMAREHQDIVANDDSRQAKQEQERAERDYELFLQELEEDEELRQTINLYKAGENAPVKSHGDDEDEEDEDAPEIGIDELLDELDDMTLEDTPMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHG0</accession>
    <name>G3AHG0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial</fullName>
            <ecNumber evidence="10">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Succinyl-CoA synthetase beta chain</fullName>
            <shortName evidence="10">SCS-beta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LSC2</name>
        <name evidence="14" type="ORF">SPAPADRAFT_134214</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 12">Belongs to the succinate/malate CoA ligase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="10"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373708.1">
        <property type="nucleotide sequence ID" value="XM_007373646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHG0"/>
    <dbReference type="FunCoup" id="G3AHG0">
        <property type="interactions" value="747"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHG0"/>
    <dbReference type="GeneID" id="18869791"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134214"/>
    <dbReference type="eggNOG" id="KOG2799">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037430_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHG0"/>
    <dbReference type="OMA" id="ITACDEV"/>
    <dbReference type="OrthoDB" id="1552at2759"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042709">
        <property type="term" value="C:succinate-CoA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006104">
        <property type="term" value="P:succinyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000001">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000004">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00558">
        <property type="entry name" value="Succ_CoA_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_ligase-like_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001913">
        <property type="entry name" value="PRK00696.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01016">
        <property type="entry name" value="sucCoAbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815:SF1">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP-FORMING] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="SUCCINYL-COA SYNTHETASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2 10" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="ATP-grasp" evidence="13">
        <location>
            <begin position="28"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="72"/>
            <end position="74"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="347"/>
            <end position="349"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="415" mass="44564" checksum="C30837DD269ABAF0" modified="2011-11-16" version="1">MLSRSIARVSRAAAQQKRFLSLHEYRSAALLQQYGVPIPRGFAATTPEGAFEAAEKLGTSELVIKAQALTGGRGKGHFDSGLQGGVKLISSAQEAKDLAQQMLGHKIITKQTGAAGKEVTAVYIVERRDAATEAYLAILMDRASQTPIIVASAQGGMDIEGVAAENPDAIKTFQVPLKEGVSDDLGAQIANVLGFTQEAIPEAVKTIQGLYQCFIDRDCTQVEINPLSETPDHKVLAMDAKLGFDDNAEFRQEEVFSWRDTTQEDPQEVEASKYGLNFIKLDGNIANIVNGAGLAMATMDIIKLYGGEPANFLDCGGTATPETIEKAFGLILSDKKVNGIFVNIFGGIVRCDYVAEGLIAAAHTFKLDIPVVVRLQGTNMEKAKELIENSGLKLYAFEELDPAAEKIVQLAPKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHK5</accession>
    <name>G3AHK5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Chromatin-remodeling ATPase INO80</fullName>
            <ecNumber evidence="15">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">SPAPADRAFT_49234</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">ATPase component of the INO80 complex which remodels chromatin by shifting nucleosomes and is involved in DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 23">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the INO80 chromatin-remodeling complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The DBINO region is involved in binding to DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="15"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373753.1">
        <property type="nucleotide sequence ID" value="XM_007373691.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AHK5">
        <property type="interactions" value="1117"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHK5"/>
    <dbReference type="GeneID" id="18871319"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49234"/>
    <dbReference type="eggNOG" id="KOG0388">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_26_2_1"/>
    <dbReference type="InParanoid" id="G3AHK5"/>
    <dbReference type="OMA" id="FWKKNER"/>
    <dbReference type="OrthoDB" id="372624at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032006">
        <property type="term" value="P:regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18002">
        <property type="entry name" value="DEXQc_INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000022">
        <property type="entry name" value="Blast:Putative DNA helicase Ino80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001269">
        <property type="entry name" value="SNF2 family helicase/ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020838">
        <property type="entry name" value="DBINO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031047">
        <property type="entry name" value="DEXQc_INO80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF2">
        <property type="entry name" value="CHROMATIN-REMODELING ATPASE INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13892">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51413">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="21" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DBINO" evidence="20">
        <location>
            <begin position="306"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="549"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="1111"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="181"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1284"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="388"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="185"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="209"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="239"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1287"/>
            <end position="1296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1310"/>
            <end position="1329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EGW34169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1340" mass="154518" checksum="BD815658C3864D36" modified="2011-11-16" version="1">MNISSMLSNDSTVPTPTTQSPLHVTTDQTPIPTTSLLQGNNTVPNPSSTVANLVPVSAASTGTENGHGQHLSREDSIVSDIISTHNYNNNDTYDVAYKTKFESNFNILNQLDQYQQLSHNLKLLKFDEIKLNNFIINTNKFTSNYIDRVIAEDISSRNNKISDTNKSKELQLIDYSKPLHHNTTRGKEFKWGSTREVHKVESKKKRRVASSSTTAASPNGSSGPVLIKPKLGDNVAVRRSTRPKPKRKLPVEEQTEEEEDHHKKVKLEQDGKLKIKLSVKKPEPVNVNSKEYKAFLRQYDNTCIAIWKDMSRKDGPKVSRLMQQSTQAKLINLKKTSILAAREAKRWQLKNNKNQKDLTTKARRAMREMFNFWKRNERIERELRKKYEKELLDKAKREEEEREAKRQSRKLNFLITQTELYSHFIGKKIKTDEIEGSDSDPNLVKHDKSHLDKYTDIESKSHDINTLDFENEDDDALHRAAAQNAQNALELAQSRAKEFNDEPLKNPDTNGEEMNFQNPTLLGDITIPQPKMLTCTLKEYQIKGLNWLANLYEQGINGILADEMGLGKTVQSISVLSYLAETHNIWGPFLVVTPASTLHNWQQEISRFVPNFKVLPYWGNAKDRKILRKFWDRKSLRYTKDSPFHVLVTSYQLVVADAAYFQKMKWQYMILDEAQAIKSSQSSRWKSLLSFSCRNRLLLTGTPIQNSMQELWALLHFIMPSLFDSHDEFSDWFSKDIESHAQSNTSLDEQQLRRLHVILKPFMLRRIKKNVQSELGDKKEIDIICDLTNRQQKYYQMLRSQISIMDLLDSSSLNSSSSDDATSLINLVMQFRKVCNHPDLFERADVKSPFSFGKFAETSSFLRETNELEMSYTTENVVSYNLPKLIYDELLTPNEKTDSKDSIYEMFNIYKPEHVKDNQQFGWLKSIDVSPQELNTYTRRHIVERAIDLQEYTDINYSKVSRFNYLYEGEFVPNNKKLLITQYENKFSNITNSSILSKLYSIQDHVYEDMFINVISPAAEPLASAPPITSVCSSRNFYYKTQDVLFNPKIRSSLLPLPLSQEYQLMTENTPLESYPKSNMLPSPVNKKIDYSNIRMPSMNRFITESGKLAKLDELLVELKQEGHRVLIYFQMTRMMDLMEEYLTYRQHKYIRLDGSSKLDDRRDLVHDWQTKPELFIFLLSTRAGGLGINLTAADTVIFYDSDWNPTIDSQAMDRAHRLGQTKQVTVYRLLTRGTIEERMRDRAKQKEQVQQVVMEGKATITKKEEHINKKKDVAFLLLGDSPSDVSSGKTSTTSTPEPESANGKKKYRVSADDALSSKKLQELYHEGEGEFSGNVTPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHL9</accession>
    <name>G3AHL9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_59615</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373767.1">
        <property type="nucleotide sequence ID" value="XM_007373705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHL9"/>
    <dbReference type="GeneID" id="18872989"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59615"/>
    <dbReference type="HOGENOM" id="CLU_1349639_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHL9"/>
    <dbReference type="OMA" id="MREAMVN"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442513" evidence="2">
        <location>
            <begin position="25"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="162"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="203" mass="20546" checksum="CC1AB201D56BC2F5" modified="2011-11-16" version="1">MKIATLALALTAAVSINAAAIVEGNKIAQGLVVRADDTTTDAAAAADETDDTDDSDDTDDADDADETDDTDDSDDTDDADDADETDDTDDSDDTDDADETDDADDSDDTDDSDETDDSDDSDETDDSDDSDETDDSDDSTETDDSDDSTETGAQDTATVGHANASGNATTSGNATTTGKGIAAGMYPSFGMAIAAAAVGAVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHN0</accession>
    <name>G3AHN0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase</fullName>
            <ecNumber evidence="4 14">4.2.1.134</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_59628</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a very-long-chain (3R)-3-hydroxyacyl-CoA = a very-long-chain (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:45812"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:83728"/>
            <dbReference type="ChEBI" id="CHEBI:85440"/>
            <dbReference type="EC" id="4.2.1.134"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endoplasmic reticulum membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the very long-chain fatty acids dehydratase HACD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.134" evidence="4 14"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373777.1">
        <property type="nucleotide sequence ID" value="XM_007373715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHN0"/>
    <dbReference type="FunCoup" id="G3AHN0">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHN0"/>
    <dbReference type="GeneID" id="18872995"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59628"/>
    <dbReference type="eggNOG" id="ENOG502SF4K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1209709_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHN0"/>
    <dbReference type="OMA" id="PKIAKHT"/>
    <dbReference type="OrthoDB" id="46988at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102158">
        <property type="term" value="F:very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030497">
        <property type="term" value="P:fatty acid elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007482">
        <property type="entry name" value="Tyr_Pase-like_PTPLA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035:SF3">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04387">
        <property type="entry name" value="PTPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 14" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7 14" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="217"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="225"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW34193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="244" mass="28109" checksum="3B787A3F58B57E16" modified="2011-11-16" version="1">MDRFYPLPTNQKVVFFFNSAAATLWFCCLLRFLLLLPLVGRRFLPGGIADFFQVVALLPLIGFLLVKPLLNKKISLSNLWALSNDLKMAWICYGVIFPHPKIAKHTSYSILISAWCVQYFIHYSYHAFRIKTKSSPHFLFWLQYHNFYVIYPMALVAEMILTFLSLGFVQENSIHEIALKATLLSYIPVAYFAWGHLQARKKNKYIEVMNKRRESAMKTAPSAEIATTSGLSPNTQSTELREIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHT9</accession>
    <name>G3AHT9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nucleolar protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_59678</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373837.1">
        <property type="nucleotide sequence ID" value="XM_007373775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHT9"/>
    <dbReference type="FunCoup" id="G3AHT9">
        <property type="interactions" value="775"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHT9"/>
    <dbReference type="GeneID" id="18873020"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59678"/>
    <dbReference type="eggNOG" id="KOG1122">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005316_3_1_1"/>
    <dbReference type="InParanoid" id="G3AHT9"/>
    <dbReference type="OMA" id="PIGSWTK"/>
    <dbReference type="OrthoDB" id="427002at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009383">
        <property type="term" value="F:rRNA (cytosine-C5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1170:FF:000001">
        <property type="entry name" value="Ribosomal RNA methyltransferase Nop2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011023">
        <property type="entry name" value="Nop2p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023273">
        <property type="entry name" value="RCMT_NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="RsmB/NOL1/NOP2-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00446">
        <property type="entry name" value="nop2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF30">
        <property type="entry name" value="28S RRNA (CYTOSINE(4447)-C(5))-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02012">
        <property type="entry name" value="RCMTNOP2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="13">
        <location>
            <begin position="244"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="131"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="36"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="461"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="336"/>
            <end position="342"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="581" mass="65249" checksum="CDCF59F0B7BF2A73" modified="2011-11-16" version="1" fragment="single">MGRRAKNKQGVPPTLDEFQAKQVKKEKRKREDKPEPKHKKPVEHKKSKKQKVEVEQEPEPELPEVDLEELASARKSLFDESDEEEPQDDEFDIDGEDLDSDEEERAHPMFSDDEGSEDDLEGLNAANMEAYSRQLDEEDELEAEEAELELLEAEKAQPRAAVLPTAEEEAEIAKGGPQDVTAVRTRMLEIVKVLENFNDMAEEGKSRTDYVNRLLADICEYFGYSEFLADKLFHLFSPAEAMEFFEANEIARPITIRTNTLKTRRRDLAQKLVNRGVNLQPIGSWTKVGLQIFDSQVPIGATPEYLAGHYILQAASSFLPVMALAPQENERVLDMAAAPGGKTTYISALMKNTGCVFANDANKARTKSLIANIHRLGCKNTIVCNYDAREFPKVIGGFDRVLLDAPCSGTGVIAKDEGVKVRRTEKDFIQIPHLQKQLLLSAIDSVDANSPTGGIIVYSTCSVAVDENESVVDYALRKRPNVKLVDTELQIGKEGFTSYRGKHFNPKLSLTRRYYPHTYNVDGFFVAKFKKIAPSPHDVSKAGAKEKESLIRQEYEDEGIIHDDFAEFDEDADKEIVESSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHU6</accession>
    <name>G3AHU6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_59682</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373844.1">
        <property type="nucleotide sequence ID" value="XM_007373782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHU6"/>
    <dbReference type="FunCoup" id="G3AHU6">
        <property type="interactions" value="1342"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHU6"/>
    <dbReference type="GeneID" id="18873024"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59682"/>
    <dbReference type="eggNOG" id="KOG1678">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080796_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHU6"/>
    <dbReference type="OMA" id="YIRDAWK"/>
    <dbReference type="OrthoDB" id="10255148at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1120.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1120.10">
        <property type="entry name" value="Ribosomal protein l15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024794">
        <property type="entry name" value="Rbsml_eL15_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000439">
        <property type="entry name" value="Ribosomal_eL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020925">
        <property type="entry name" value="Ribosomal_eL15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003269">
        <property type="entry name" value="PRK04243.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847">
        <property type="entry name" value="RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00827">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01384">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01194">
        <property type="entry name" value="RIBOSOMAL_L15E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="161"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="204" mass="24351" checksum="2BD6FB75CCCDDD37" modified="2011-11-16" version="1">MGAYKYLEELQRKKQSDVMRFLYRVRCWEYRQKNVIHRASRPSRPDKARRLGYKAKQGFVIFRVRVRRGGRKRPVPKGATYGKPTNQGVNQLKYQRSLRSTAEERVGRRAGNLRVLNSYWINQDSTYKYFEVILVDPSHKAIRRDARYNWITEPVHKHRESRGLTAAGKKSRGINKGHRYTKTKAGRRHTWKKNNTLSLWRYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHV0</accession>
    <name>G3AHV0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_59685</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the cytochrome b5 family. MAPR subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373848.1">
        <property type="nucleotide sequence ID" value="XM_007373786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHV0"/>
    <dbReference type="FunCoup" id="G3AHV0">
        <property type="interactions" value="872"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHV0"/>
    <dbReference type="GeneID" id="18873026"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59685"/>
    <dbReference type="eggNOG" id="KOG1110">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042860_1_2_1"/>
    <dbReference type="InParanoid" id="G3AHV0"/>
    <dbReference type="OMA" id="WELQFRE"/>
    <dbReference type="OrthoDB" id="547796at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000003">
        <property type="entry name" value="membrane-associated progesterone receptor component 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050577">
        <property type="entry name" value="MAPR/NEUFC/NENF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281:SF72">
        <property type="entry name" value="NEUDESIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442413" description="Cytochrome b5 heme-binding domain-containing protein" evidence="7">
        <location>
            <begin position="18"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="8">
        <location>
            <begin position="41"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="156" mass="17585" checksum="A615408DB45C8EB6" modified="2011-11-16" version="1">MITTVLILLIILYLARSFYKDHVENPIDATPEESTVVEGNFTPKTLAKYNGKDDPKVFLAVKRVVYDVTMGKSFYGPGGPYENFAGRDASRGLAKNSFDLEMLTPLDQPIDKLGDLNKEELESLANWEDLFENKYKVVGKLYENDEVVNGDVVKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHW5</accession>
    <name>G3AHW5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Polyamine transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_134046</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373863.1">
        <property type="nucleotide sequence ID" value="XM_007373801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHW5"/>
    <dbReference type="FunCoup" id="G3AHW5">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AHW5"/>
    <dbReference type="GeneID" id="18869779"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134046"/>
    <dbReference type="eggNOG" id="KOG0255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008455_11_3_1"/>
    <dbReference type="InParanoid" id="G3AHW5"/>
    <dbReference type="OMA" id="LMPETHK"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000297">
        <property type="term" value="F:spermine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140115">
        <property type="term" value="P:export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042908">
        <property type="term" value="P:xenobiotic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF38">
        <property type="entry name" value="POLYAMINE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="26"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="374"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="435"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="27"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="509" mass="56146" checksum="15F7286E7F493633" modified="2011-11-16" version="1" fragment="single">DVKELDYDDAQDKDNPHNWPAWKKWMVTYTVAFMCLCCSLGSSMYTGGVPEIMIRFGASQELSLAGLTFYLIGLALGPAIAAPLSEVFGRRWIYILSLPIAMLFTMGVGLAKNMGTILVLRFFCGYFASPAMAMGGGTISDIWSKEPAEQSFAIALFCLCPFLGPVLGPVIGGFAGEHKNWQWAASWVLLMFFGFVWPFVILSPETYKPVILKNRAIKRGMKVNAPKINSAFIKRVIFFDLLRPIQLLFKEPIVFFFSVYVSFIFAVLFAFFEAFPVIFRGVYHMDLGVSGLTFISVGIGLILGIAFYVVLDKVYYFPKNPDGTRGKRDENGNIVWDAPETKLLVAQIGSVFLPISLFWLGWTAKPSIHYMAPVAAGIPFGFGLILVFLAVVLYFSMSFPPMNVASAIAANNLLRYLLASVFPLFTVQCYENLGIGWASSLFAFIALAMVPVPFIFSYFGPRLRAKSKYGYAAFFKKLAEEKAAAAAAAASAAPAEKQEATEDVVSDKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHY3</accession>
    <name>G3AHY3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Domain of unknown function at the cortex 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59722</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373881.1">
        <property type="nucleotide sequence ID" value="XM_007373819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHY3"/>
    <dbReference type="STRING" id="619300.G3AHY3"/>
    <dbReference type="GeneID" id="18873045"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59722"/>
    <dbReference type="eggNOG" id="ENOG502RXNE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047849_0_1_1"/>
    <dbReference type="InParanoid" id="G3AHY3"/>
    <dbReference type="OMA" id="NHELRYT"/>
    <dbReference type="OrthoDB" id="2119945at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013897">
        <property type="entry name" value="Duc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34826">
        <property type="entry name" value="UPF0590 PROTEIN C409.17C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34826:SF2">
        <property type="entry name" value="UPF0590 PROTEIN C409.17C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08588">
        <property type="entry name" value="Duc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Domain of unknown function at the cortex 1" evidence="1">
        <location>
            <begin position="6"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="293" mass="33476" checksum="1B62B3F563483B78" modified="2011-11-16" version="1">MAIVHRLYITASNNYSENFQIVAVNTTIPLEIDSEIGVFKVVVNIKDFDGSKPHLSNSCYNIDDDTFLDGEPLNFKRNEHDNIIPNVRMQIEFTPKKDINGAQLIFGNDFTYPVKKYIPTMLLTTGLKLYNLFVSNSVQGNLYSNKPYLYGLALNNFTYMGIDNFMQIPKIINSKTVPMDCEENVSSQLHTLTHSTSQGRIKYFNQREDCEEFVFKQGVAYNLQFDTNYVKMSNSNYAICIPKFDVNVNRYVNETLNNFNYVIKEGGHTNVDEGTAGLVINFALRAEEEHLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI29</accession>
    <name>G3AI29_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_70471</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373927.1">
        <property type="nucleotide sequence ID" value="XM_007373865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI29"/>
    <dbReference type="FunCoup" id="G3AI29">
        <property type="interactions" value="124"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AI29"/>
    <dbReference type="GeneID" id="18875328"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70471"/>
    <dbReference type="eggNOG" id="KOG3322">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007205_0_1_1"/>
    <dbReference type="InParanoid" id="G3AI29"/>
    <dbReference type="OMA" id="HDRARIY"/>
    <dbReference type="OrthoDB" id="442863at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005697">
        <property type="term" value="C:telomerase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000171">
        <property type="term" value="F:ribonuclease MRP activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034965">
        <property type="term" value="P:intronic box C/D snoRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000294">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, RNase MRP-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039182">
        <property type="entry name" value="Pop1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009723">
        <property type="entry name" value="Pop1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012590">
        <property type="entry name" value="POPLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22731">
        <property type="entry name" value="RIBONUCLEASES P/MRP PROTEIN SUBUNIT POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22731:SF3">
        <property type="entry name" value="RIBONUCLEASES P_MRP PROTEIN SUBUNIT POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06978">
        <property type="entry name" value="POP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08170">
        <property type="entry name" value="POPLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pop1 N-terminal" evidence="4">
        <location>
            <begin position="39"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="POPLD" evidence="5">
        <location>
            <begin position="479"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="746" mass="85357" checksum="14DA3F8CFE2D1A75" modified="2011-11-16" version="1">MSNPNINRKKARLYNSRNIRAQSTDPSLDSKEKLSVPTFLASRQFEIKSFELSQLNSKNALSTRIFQSLPRSLRRRAASHNVKRIPKRLRVRAVREMSATGPVKRMPKGRQLYKIQSAKRLLRVAGRLKSMKQIPEFKSVNIRQQYKLLSKQLKELVENKKLPQVNNAVGSRDVVGINKLTNAPSGNIKYFKRQRDFVWLPTHIWHAKRFKMIKNHGYQIPHTPTQKCFKSMSRQGKTKAIAFDTSYYGTMIINIPDKQSYKNVLHDLIRTKSTKVVNGEKSYYGWIYMNSQQIYQGYVYSSLANGQIIIRVFPSVYKKLFKHFKENVNCTIQDCRFALGSIELTGPAALSSLSKMLHLHDSKTIEIWKQLTSVQDTDLIPIGTTFTFDSQDPRIFKSFKMYKSDTTNIYDVIISLQSTQLVDPSIVNQICQSESRNNSYKDQLTNKDIGKFHANLTSQPKDISKSHIPALVSKLARQKWMIILPWHWILPFWLTLAKIRDVKPGGIQQIYQLNFENGVGSFPQDFPWLEDGWIYNDINGQANLFKASKLPKSQVTLAAAESKVSDVFDAYKCDWHSLRNITYIQKLKNLPEKLESKTNYASFEGLERNITSTHDLIQVVRSLKEEIVDSEPVIELYDETNPNHKQFHDNSSTIESIPAIVRSKLPVRQVRVTLIGEGTISNNARVYSDKDACDVNVIGFVTSGGMNLRQGAFTGIGAIVAQEFSSQIYIRNVGQTKLYLGKLELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIE4</accession>
    <name>G3AIE4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Leucine rich repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_59846</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373998.1">
        <property type="nucleotide sequence ID" value="XM_007373936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIE4"/>
    <dbReference type="GeneID" id="18873100"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59846"/>
    <dbReference type="eggNOG" id="ENOG502T36X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_794797_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIE4"/>
    <dbReference type="OMA" id="WELNYTK"/>
    <dbReference type="OrthoDB" id="27267at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF22">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN COMPLEX ACID LABILE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="341" mass="40061" checksum="AF0E98B65026975A" modified="2011-11-16" version="1">MILLPDEIIVNIFKYLSSKETIKLIKKLEWDPRFTAIVDLLYQRLYHGRLLIINEDPKQKFESDYELTVDSFEDMFTSTTHEHSLFRTIRPNYVEFKFSRNASDYRTFINTLYKFHTLLTRGSLEVQDYFENLILQVNFYIDANLVLIENPNTLTTIIIKILLELSDNIHLVSKIKEFTIKCTDIGAFYQSKWSNYLSRFTSLEYLDLSQNLLQSSNYQEQEFDIWGKEKKFPDGLKELKLDFNMFTEITKEFITKLPNSLQSLSLNYNTIEMIEPCSIGVLLPNLKCLDLNYNNLTALEPSIFKDCARGFDLRLKASHLDDTTVDQLKILAKKNGYKVTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIH8</accession>
    <name>G3AIH8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAD-dependent protein deacetylase</fullName>
            <ecNumber evidence="6">2.3.1.286</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_149340</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(6)-acetyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-acetyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:43636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="ChEBI" id="CHEBI:83767"/>
            <dbReference type="EC" id="2.3.1.286"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.286" evidence="6"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374032.1">
        <property type="nucleotide sequence ID" value="XM_007373970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIH8"/>
    <dbReference type="FunCoup" id="G3AIH8">
        <property type="interactions" value="457"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIH8"/>
    <dbReference type="GeneID" id="18870821"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_149340"/>
    <dbReference type="eggNOG" id="KOG2682">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023643_7_2_1"/>
    <dbReference type="InParanoid" id="G3AIH8"/>
    <dbReference type="OMA" id="ATHSCID"/>
    <dbReference type="OrthoDB" id="420264at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099115">
        <property type="term" value="C:chromosome, subtelomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031934">
        <property type="term" value="C:mating-type region heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005721">
        <property type="term" value="C:pericentric heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033553">
        <property type="term" value="C:rDNA heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046970">
        <property type="term" value="F:histone H4K16 deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045950">
        <property type="term" value="P:negative regulation of mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031508">
        <property type="term" value="P:pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017328">
        <property type="entry name" value="Sirtuin_class_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF6">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACETYLASE SIRTUIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037938">
        <property type="entry name" value="SIR2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6 8" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 6 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="11">
        <location>
            <begin position="1"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7 10">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="27"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="37"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="109"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="205"/>
            <end position="206"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="229"/>
            <end position="231"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR037938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR037938-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR037938-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR037938-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW34448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="363" mass="41076" checksum="D4EEA62CD28157DB" modified="2011-11-16" version="1">MSQLEKKLKPIVDAINKGKKVTFFNGAGISTGAGIPDFRSPETGLYANLAKLNLPFAEAVFDIDFFEDNPKPFYTLAEELYPGKYAPTKFHYLIKLLQDQDSLLRVYTQNIDTLERLAGVEDDYIVEAHGSFARNHCIKCHREMDNETLKKHMHDETKDGIPTCETCQGYVKPDIVFFGEGLPGRFFQQWDEDCDDVEIALVAGTSLTVYPFASLPASLGKKSLRVLINKEKVGDFSDNYRKSDIVILEDCELFAEQLSSMLGLRDELEALVEQSKSKFGTGKHISPETTVDEIEEKLKVEAHLHEKKEDDDITELTEKTEKIGLTEETSFNNKEKEEKAKARAEAKEKAKIEKLEEIANTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIV4</accession>
    <name>G3AIV4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59126</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373349.1">
        <property type="nucleotide sequence ID" value="XM_007373287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIV4"/>
    <dbReference type="GeneID" id="18872786"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59126"/>
    <dbReference type="eggNOG" id="ENOG502SFF8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_852572_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIV4"/>
    <dbReference type="OMA" id="DTNCSMA"/>
    <dbReference type="OrthoDB" id="5377012at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="280" mass="30486" checksum="EC7534BD06607748" modified="2011-11-16" version="1">MSSPEINNYTGDKRQAPRNRFRWVRRLMQGQNRVHHVNLANQHDSNSQRPQTTSSDTHMRNTHAPRTRTRRSASDSATKSSEDNQGINSDEEGNADLTIRDFTSPDDRSIGALSDQISDNISTIPLKSIISSQSTKSASIISDNQHDQTSLIASTAETSLAPSSNTNYTFAPNNNTGAGTPTGITFGNTLSVASPIGLDRDSESIVTLASSSRRIRRRSIDTNCSTTAIPPASIMERLSVQPTVGNSSTYATSVRTSDRTSQFDASQHYDDQSSSVKSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ69</accession>
    <name>G3AJ69_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_65052</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373410.1">
        <property type="nucleotide sequence ID" value="XM_007373348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ69"/>
    <dbReference type="GeneID" id="18875013"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65052"/>
    <dbReference type="HOGENOM" id="CLU_1354435_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ69"/>
    <dbReference type="OMA" id="HENVQGQ"/>
    <dbReference type="OrthoDB" id="4023585at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="205" mass="22304" checksum="267346CA751BA82B" modified="2011-11-16" version="1">MFRLVTRSYVTKEGIANSIKEAGKTVKETILGGANQAEKTLKDKVGDAQEAGASLKDKLSEKTSQAADTIKDKAGEAQETGASIRDQVKGETKQAGKTIKDQVDETQSAGASVRDKLVGEGKAATKSMTNEAIEQGKQAKETIKDKWNNPKQATEEFKDKFIGEKPEAIKDRQTARVRDNYKGYASLQDKGRKAPSEQNRPEDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJB2</accession>
    <name>G3AJB2_SPAPN</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_65695</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374155.1">
        <property type="nucleotide sequence ID" value="XM_007374093.1"/>
    </dbReference>
    <dbReference type="GeneID" id="18875058"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65695"/>
    <dbReference type="eggNOG" id="ENOG502SF90">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_240775_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJB2"/>
    <dbReference type="OMA" id="YINHMLA"/>
    <dbReference type="OrthoDB" id="4018542at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019021">
        <property type="entry name" value="Mms22"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09462">
        <property type="entry name" value="Mus7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="471"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="517"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1419" mass="164033" checksum="EA9C2B75185E4930" modified="2011-11-16" version="1">MSYECISDSEEEESSHLVISPQLLENNLHTLSPNKRSTTRDRLRQYLQQNASVQEQLIEVNPPVSDISSSELPTVTMIPSHHSPQTTELDEDSHHLQTYINSRATSTRRGLRKRNFASTHPYLSDQAHYLGLCDISYLNELYDENNQDLDVIVKYLNYNYMKMKERYRGDEKYRHKNFYTILGRGLNSLRTEENQGETYHDTQVESQSETQWHKEQDSISGFMNDLFSDSDQDNYSQSEDSDLEPIPTHKHHSLVIEDDEEQSDSELSDSDALVRVGGRIRKEKSILRGALPESAKRLSIYKAPAYKRKTKSTPKQMDYRKGMAIRKSAPKHRSIPEFTGFVDDSITYDTSNELYHELYNQPEDEPILERLESDPPSMYDAVVLSESSSSDNESGDESDIFEYGHPDIDTHTDAIDLTNITDDEFGEVQERDSINMMLATGERRHGPNTKKKHRSTQSTLPTAYRPPTYGSSANTSSAHTSSAYGSSSTVRNATNNTSRKRLNSSHSSSTSTKTRKQNPQSYGRLQVKSISPATNQRKKHKSQKKNGIIDTYLSKNPKDLFANDYLFSRQPILSTTTIEAESETRFVKEPIHSGERNHNNNSLSKPLLNSIDLAKIIELAQGKSYTLAKDTVNITFLNETYTLSLVDIQRSTSTNERLLTQLAKTTKSFNVPLELFSQAVSECIKGLIEWALVLQRSFSTREWKLLDICYNSLVKSVVIPISFKLQHMPYFMLLYYTVSNIALFQGGSPVDLNDFIKRYAQSYWKLYFDRFSIYETFDEDESFTVHCMLVNRNLWWESIVSTLQNYDSPNSSTLLEPLFQLCSNTNNKGYSWVPVSILFDKHDNSTNIDFCYRYIETIYMLNQRKDWPIDEKVILQIYRSIAARKFANFTDEIEVPDLLGRVRTRYDIPDSSFFERFMQFLYWYVSGISHAQTVKKLITKLFISSKFQYVNDKTHRVMFINRLNFVVLLSQLSNSVDLGNQICGLFEAIEQPKDVELFKLGMSSLIVLCQVAVSTHSKLPIDAMVIFIKFCVHNFFTVSGTFRLWNRFIHLFKDKIVKNLNANQMIQVLGLSKGLHIDMPDLIWQDLLEMFAHICPVSTPVSQTCKVIQGLVDLLERVLHRQMGRIPLPSLQEETRVCTLIESCIANWIKFSALLPGTNWEKLVLQTFPYTGNHASREKFSIYFYFNVLQYHNLKGCQELVGRCVIRELMLYNPSKYLSSILKVLEREKWGLFALSNEIDVRYLIQHSRHIIVNNIIDNLSKSRMKSSEKLRYVNEIQKVVNSELDKYYSCNWYKEFCVRVVKCIQIQCQDFIDDATGKSLSSLSSKLGISQLELSQFKIHKMPILDKLRMFHIDFVNAVHFGRDVLEILDKFVMGGVDVLYHLMSVYLEAISARQYDKWKLVYELLKYFDTNYQECQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJL4</accession>
    <name>G3AJL4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_148144</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373497.1">
        <property type="nucleotide sequence ID" value="XM_007373435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJL4"/>
    <dbReference type="FunCoup" id="G3AJL4">
        <property type="interactions" value="410"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJL4"/>
    <dbReference type="GeneID" id="18870777"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_148144"/>
    <dbReference type="eggNOG" id="KOG0730">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_12_3_1"/>
    <dbReference type="InParanoid" id="G3AJL4"/>
    <dbReference type="OMA" id="DRHIYVA"/>
    <dbReference type="OrthoDB" id="27435at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034214">
        <property type="term" value="P:protein hexamerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19503">
        <property type="entry name" value="RecA-like_CDC48_NLV2_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19511">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001721">
        <property type="entry name" value="AAA family ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF27">
        <property type="entry name" value="ATPASE FAMILY GENE 2 PROTEIN HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="251"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="520"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW33913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="750" mass="81441" checksum="6C55B0EA05177179" modified="2011-11-16" version="1">MSSKKKKTPSGSTPTPVATSTDPPKKIKLPKSYIVRPHSSPSGKTISHIILNAEIIPSLALSIGEFILVSRDGSPGIVGVVQAGDVERNMIEVSDEFRQLAGFLLGDRVDFTSYTRQPPYAKCVYVSEDSDKKLDKKTLDDIGLVYPGLNVNGSVIVDIDSSLESSLDKLSLNEAAADTEVVSPPYIFVKSTTKLEPTSQTTPPTSKYPYLPDPITYTSVGGLTKQIQLLQQTISLPLHSPTLFSDFGISPPRGILLHGPPGTGKTMLLRCAANTSNAHVLTINGPSIVSKYLGETENTIRDIFAEARKYQPSIIFMDEIDALVPSRTGSDAGETESRVVAQLLTMMDGMDNGGRVVIVGATNRPNSIDIALRRPGRFDTEVEIGIPDIDARTDILSKLFDRMNHDKYSLTKSEIELVASKTHGYVGADLSALCREAVMNAIKQGLAQGTPQHEIKVTVDHLLAAYPDIRPSAMREILLEMPKVHWTDIGGQHELKQKLIEVVQLPLQASASFSKLGISAPKGVLLYGPPGCSKTLTAKALATESGLNFLAVKGPEIFNKYVGESERTIREIFRKARAASPSIIFFDEIDAIASVRDSSTDASSNVLTSLLNEIDGVEELKGVVIIGATNKPSDIDPALLRPGRLDRHIYVAPPDFEARVQILEKCCSKFDLDQNEVDLQQLAKLTEGCSGAEVTLLCQEAGLAAIMEDNQCSTVHKRHFDHALVGIARGITHEMLQYYQDFANRSGLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJL5</accession>
    <name>G3AJL5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_65120</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373500.1">
        <property type="nucleotide sequence ID" value="XM_007373438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJL5"/>
    <dbReference type="GeneID" id="18875020"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_65120"/>
    <dbReference type="eggNOG" id="ENOG502RQEQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1372961_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJL5"/>
    <dbReference type="OMA" id="HINHNAG"/>
    <dbReference type="OrthoDB" id="4014468at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="22904" checksum="4CB1F36966BEF4F5" modified="2011-11-16" version="1">MVSRLHTLLEPIKPVPKYLKKLASQKVTAHPIQTYIPHLEFTSRDLTAISQGLTNNTLTINTQPYQTALLRQLGKQTLLFRLYTSTIFSGQRYLASSPYEIKNDLGYFTNDWVIPQVMRRNGIYDCCVVDDRLVTTERIEYERYLNKVKEVKDKTAIGSLFTLIGLVKVKYGDKEAKQVCDKLVEGKRGIIEIVQEEMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJR4</accession>
    <name>G3AJR4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_59376</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373549.1">
        <property type="nucleotide sequence ID" value="XM_007373487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJR4"/>
    <dbReference type="GeneID" id="18872886"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59376"/>
    <dbReference type="HOGENOM" id="CLU_2470501_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJR4"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="88" mass="10030" checksum="3A777ECAAE0B7856" modified="2011-11-16" version="1">MENPSIKTDTKTNTQKNAGRHGRARKVSNSSIESDVVIIGSGLAASLMHVQLWFKRRKKHNHKNRHKKSKQTHTNHTLENVAIDSETY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJV9</accession>
    <name>G3AJV9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Pyrimidine-specific carbamoyl phosphate synthase-aspartate carbamoyl transferase</fullName>
            <ecNumber evidence="6">2.1.3.2</ecNumber>
            <ecNumber evidence="5">3.5.1.2</ecNumber>
            <ecNumber evidence="19">6.3.4.16</ecNumber>
            <ecNumber evidence="4">6.3.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="ORF">SPAPADRAFT_49095</name>
    </gene>
    <organism evidence="30">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="24">Multifunctional protein that encodes the first 2 enzymatic activities of the de novo pyrimidine pathway: carbamoylphosphate synthetase (CPSase; EC 6.3.5.5) and aspartate transcarbamylase (ATCase; EC 2.1.3.2). The CPSase-function is accomplished in 2 steps, by a glutamine-dependent amidotransferase activity (GATase) that binds and cleaves glutamine to produce ammonia, followed by an ammonium-dependent carbamoyl phosphate synthetase, which reacts with the ammonia, hydrogencarbonate and ATP to form carbamoyl phosphate. The endogenously produced carbamoyl phosphate is sequestered and channeled to the ATCase active site. ATCase then catalyzes the formation of carbamoyl-L-aspartate from L-aspartate and carbamoyl phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23 31">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18029"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">In the 2nd section; belongs to the CarB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">In the 3rd section; belongs to the metallo-dependent hydrolases superfamily. DHOase family. CAD subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">In the C-terminal section; belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">In the N-terminal section; belongs to the CarA family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="6"/>
    <dbReference type="EC" id="3.5.1.2" evidence="5"/>
    <dbReference type="EC" id="6.3.4.16" evidence="19"/>
    <dbReference type="EC" id="6.3.5.5" evidence="4"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373594.1">
        <property type="nucleotide sequence ID" value="XM_007373532.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AJV9">
        <property type="interactions" value="1705"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJV9"/>
    <dbReference type="MEROPS" id="C26.956"/>
    <dbReference type="GeneID" id="18871303"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49095"/>
    <dbReference type="eggNOG" id="KOG0370">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000513_2_1_1"/>
    <dbReference type="InParanoid" id="G3AJV9"/>
    <dbReference type="OMA" id="WSPFNGK"/>
    <dbReference type="OrthoDB" id="1924069at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005951">
        <property type="term" value="C:carbamoyl-phosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004087">
        <property type="term" value="F:carbamoyl-phosphate synthase (ammonia) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01423">
        <property type="entry name" value="MGS_CPS_I_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000002">
        <property type="entry name" value="Aspartate carbamoyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000036">
        <property type="entry name" value="Aspartate carbamoyltransferase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000025">
        <property type="entry name" value="Bifunctional pyrimidine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000005">
        <property type="entry name" value="CAD protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000004">
        <property type="entry name" value="Carbamoyl-phosphate synthase (glutamine-hydrolyzing)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.20:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1030.10:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000036">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1380:FF:000009">
        <property type="entry name" value="Carbamoyl-phosphate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="entry name" value="Carbamoyl-phosphate synthase small subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="entry name" value="Carbamoyl-phosphate synthetase, large subunit oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00001">
        <property type="entry name" value="Asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CPSase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CPSase_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058047">
        <property type="entry name" value="CPSase_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002032">
        <property type="entry name" value="PRK00856.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003671">
        <property type="entry name" value="PRK05294.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009455">
        <property type="entry name" value="PRK12815.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009475">
        <property type="entry name" value="PRK12838.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF5">
        <property type="entry name" value="CAD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25596">
        <property type="entry name" value="CPSase_L_D1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00101">
        <property type="entry name" value="ATCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="Carbamoyl phosphate synthetase, large subunit connection domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="Carbamoyl phosphate synthetase, small subunit N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-grasp" evidence="27">
        <location>
            <begin position="561"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="27">
        <location>
            <begin position="1099"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="28">
        <location>
            <begin position="1356"/>
            <end position="1507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00043968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00043979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00043998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00058513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00081752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="EGW34010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="2217" mass="244892" checksum="2417EB1B44BD3DFD" modified="2011-11-16" version="1">MVQIVSAPISPPMESTGDRLMTLETHDGFAIQGYSFGAEVATAGEVVFQTGMVGYPESITDPSYEGQILVITYPLVGNYGVPDLKLMDDDYEPSLPKYFESNRIHIAGLVVAHYTEEYSHYLAKSSLGQWLKREGIPAIYGVDTRAVTKRLREKGSTLGRLCLSKPDLKSQQILADCNQHDWKQFFEVPEYDDPNVKNLVAKVSTSKPVLYTPKDGAKLGKNGKPIRIVAVDVGMKYNQIRCFVRRGVELLVVPWDYDFTKEEYDGLFISNGPGDPAVMHDTVERLSKVLAEAKTPVFGICLGHQLMARASGASTIKLKFGNRGHNIPCTSTISGRCYITSQNHGYAVDTATLTPGWKELFVNANDGSNEGVYHETKPFFSVQFHPESTPGPRDTEFLFDVFIDSVIDYNKTGVYKQVAFPGGVLAENRAAHPKVDVKKVLVLGSGGLSIGQAGEFDYSGSQAIKALKEEGIYTVLINPNIATIQTSKGLADKVYFLPVTPEFVRKVIQHERPDGIYCTFGGQTALSVGIALKDEFEGLGVKVLGTPIDTIITTEDRELFANAMAEINEKCAKSEAVNNVQEAIQAANNIGYPLIVRAAYALGGLGSGFADNEEELVSLCNKAFATSPQVLVERSMKGWKEVEYEVVRDAFDNCITVCNMENFDPLGIHTGDSIVVAPSQTLSDEDYNMLRTTAVNVIRHLGVVGECNIQYALNPFSKEYCIIEVNARLSRSSALASKATGYPLAYTAAKLGLNIPLNEIKNSVTKSTCSCFEPSLDYVVVKIPRWDLKKFTRVSALLSSSMKSVGEVMSIGRTFEEAIQKAIRSVDYQNLGFNKTAALMSIDIDQELQTPSDQRLFAIANALSDGYSVDKVWKLTNIDKWFLNKLDGLIKFGELIASFGQKEKLPLPILRQAKQLGFEDRQIAKFLDSNEMAIRRLRKDNGIIPFVKQIDTVAAEFPAFTNYLYVTYNADSSDVVFDEHGVIVLGSGVYRIGSSVEFDWCAVRAIRTLRENGTKTIMINYNPETVSTDYDEADRLYFETINLERVLDIYDLEQSAGVIISMGGQTSNNIALPLHRQNVKILGTSPEMIDSAENRYKFSRMLDKIGVDQPAWKELTSIEEAEEFAEKVSYPVLVRPSYVLSGAAMNTVYSKDDLASYLGQAVDVSPDYPVVITKYIENAKEIEMDAVAKDGKMIMHVVSEHVENAGVHSGDATLIVPPQDLDPETVRRIVEATAKIGKALDITGPYNIQFIAKDNEIKVIECNVRASRSFPFISKVVGTDLIEMATKAIMDMPVTPYPGEKLPEDYCAVKVPQFSFSRLAGADPVLGVEMASTGEVATFGRNKYEAYLKSLISTGFKLPKKNILFSIGSYKEKQELLPSIAKLHELGFKIFATAGTADFLKEHNIPVHYLEVLGKEEDQKSEYSLTQHLANNLIDLYVNLPSSNRFRRPASYMSKGYESRRMAVDYAVPLVTNVKCAKLLVEAMARKIPLEVSNRDAQTSHGTAILPGLVDITSFTTSFDEFSTTTKKALAAGFTFNSFLPHTQEGATVTDFTSLVDAIDSAGSGAYTDYSVSIAATDNNAQSVADAAELAGGLYLPFQDLSNSHISAITAHFANWPTTKPIVTDAKTTDLASVLLLASLHNRSVHVTGVLSQEDLELIKMVKNKGLSVTCDVAVLALFLSRDELDYAFLPTKKDQEYLWENLADIDCFTIGVLPYLVAKATGKAATAGVGIAETIPLLLSAVKDGKLTIADIISKFHDEPCQIFKIPKQEAQVEIDLDRFAVIEDASYPEFINKLRLRGAIERVNLHGETSVLDGKVLVDSTIGKNEVIPRNRFASITSASIPQSPTLGQKSRLASFTSDIRRPSLLRETPTPQPEFERLGAELVSQPVAGSLGEIAALSDYIRKNNNTFLRNSLLSVKDITRSDLHALFAVAQEMRLAVERQGVLDLLQGRVLATMFYEPSTRTSTSFDAAMQRLGGRVVSVDSISSSVKKGETLQDTIRTLACYSDAIVLRHPSSDSADIAAKYSPVPIINAGNGSREHPTQALLDLFTIREELGTVNGITVTFMGDLKYGRPVHSLCHLLRHYNVRVQLVSPKELAIPEDIRNFLIENKMLKLESEVLTKEIIAKSDVLYCTRVQEERFEDREQYLRLKDTYIVDNKILSDAKQHMCIMHPLPRTNEIREEVDFDQRAAYFRQMRHGLFIRMAILAMVIGVEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK15</accession>
    <name>G3AK15_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Halotolerance protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_134031</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373650.1">
        <property type="nucleotide sequence ID" value="XM_007373588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK15"/>
    <dbReference type="FunCoup" id="G3AK15">
        <property type="interactions" value="157"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AK15"/>
    <dbReference type="GeneID" id="18869776"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134031"/>
    <dbReference type="eggNOG" id="KOG0590">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_127_1_1"/>
    <dbReference type="InParanoid" id="G3AK15"/>
    <dbReference type="OMA" id="MHEMGVC"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045807">
        <property type="term" value="P:positive regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903329">
        <property type="term" value="P:regulation of iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13994">
        <property type="entry name" value="STKc_HAL4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000183">
        <property type="entry name" value="Serine/threonine-protein kinase hal4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF558">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="157"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="63"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW34066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="457" mass="51231" checksum="2C686308D6602D06" modified="2011-11-16" version="1">MPYVSHQSIRTPSTTRKPSISSSPGGGPRAPYVSLSRSGSTKVKSESPAPAAVKPRFHMLENGSHEHNLRSAKRQEKLSNMLKDFLGAKKRRDEAVSAVPDILGSPIANGTESQANAQKPPTLFASLVAQAKNDPNFNKTSDLSAGPDCRSFVEKYGRCQEVIGRGAFGVVRVSHKKNLTSSIESLYAVKEFKKRVNESDKKYHRRLTSEFCISSSLKHINIIDTLDLLKDANGDYCEVMEFCSGGDLYTLIIGQGKLEYAEADCYFKQLIRGVNYIHEMGVAHRDLKPENLLLTQNGILKITDFGNSECFKIAWEREIHLSEGICGSSPYIAPEEFTEETFDPRCVDVWSCGVIYMAMRTGRQLWKLADPEKDEFYQEYLTKRKEEGGYEPIESLKRLRCRNVIYSILDPIPGRRITTRQILNSEWGREIKVCEAGEGKCKTNEQLEGKEILVGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AK78</accession>
    <name>G3AK78_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_151797</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007374988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AK78"/>
    <dbReference type="GeneID" id="18870905"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_151797"/>
    <dbReference type="eggNOG" id="ENOG502QW50">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045443_0_0_1"/>
    <dbReference type="InParanoid" id="G3AK78"/>
    <dbReference type="OMA" id="SEREVAW"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38421:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38421">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN USGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="255"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="319" mass="36917" checksum="50D5726CFB8EB297" modified="2011-11-16" version="1">MASPILISKPVHSISTWVVLKSIELTLTSFFKSLQNAELYTKPNVILFLQSLKVSIVLYIILNIPYYFIKSMLPGEWLALCPYSVNLFMLTLCNYLGPSSQGLFLSSLKYNNMEYYKSILLVSSARHHIQYDGSWHDKLQYLFKHSRQFKLFLRRYTGFYLLNCIIFITTFFPSRISTFVLGLISFLTFLDKLGPFESAVVVAILNFCDYYYTTLILTTYYGVNNLAQDLVIPYFNEIDFTYSEQQQWLKTRQGVLFGISLVYFWLLHQCPILSMTIYLFAIMTMGYIIPSITSCPPSHLGKLQSWCTGESVWVVGSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKQ6</accession>
    <name>G3AKQ6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Manganese transporter SMF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_60295</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374475.1">
        <property type="nucleotide sequence ID" value="XM_007374413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKQ6"/>
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    </dbReference>
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    <dbReference type="GeneID" id="18873325"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60295"/>
    <dbReference type="eggNOG" id="KOG1291">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020088_4_2_1"/>
    <dbReference type="InParanoid" id="G3AKQ6"/>
    <dbReference type="OMA" id="STYLVWT"/>
    <dbReference type="OrthoDB" id="409173at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015086">
        <property type="term" value="F:cadmium ion transmembrane transporter activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015295">
        <property type="term" value="F:solute:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006825">
        <property type="term" value="P:copper ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00221">
        <property type="entry name" value="NRAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01197">
        <property type="entry name" value="nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037982">
        <property type="entry name" value="Nramp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF101">
        <property type="entry name" value="MANGANESE TRANSPORTER SMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00447">
        <property type="entry name" value="NATRESASSCMP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="126"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="299"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW32960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="464" mass="51046" checksum="6E45AAB348120540" modified="2011-11-16" version="1">MDPGNYATGITAGASNKYSLLFIVFLSNIIAIFLQSLCIKLGSVTGYDLARCCREYLPKKLNWVLWILAECAIVATDVAEVIGSAIALNILIKVPLPAGVVITIVDVLFVLMAYRADTSSMKFVKYFEWAVAVLVFGVVICFAVELSHISADAREVFRGFVPSKEMFHGNGMTIATSIIGSTVMIHSLFLGSGLVQPRLREFDVKNGYVKLDEIIDSSDEKHSSYEQEADYFYHEYKPSYQSIKYSLKYSIAELVVTLLTLALFVNSAILIVAGSTLYGTPEAIDADLYTIHDLLSRNLAPIVGTVFMLALLFSGQSAGIVCTIAGQIVSEGHINWTLKPWLRRLVTRGLSIIPCLIISLCIGRNGLGIALNISQVVISILLPPLTAPLIYFTCSKKIMRVEVSIEEAEEAGDDDNNYVVEDGRKYKYMTNNWITSGIAIIIWLFVSTLNIYAIYEMAKNGVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKR8</accession>
    <name>G3AKR8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_49902</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374487.1">
        <property type="nucleotide sequence ID" value="XM_007374425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKR8"/>
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        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="GeneID" id="18871382"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_49902"/>
    <dbReference type="eggNOG" id="KOG3780">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008578_0_1_1"/>
    <dbReference type="InParanoid" id="G3AKR8"/>
    <dbReference type="OMA" id="HWIHERS"/>
    <dbReference type="OrthoDB" id="2238745at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF174">
        <property type="entry name" value="ARRESTIN-RELATED TRAFFICKING ADAPTER 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="1">
        <location>
            <begin position="249"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="562" mass="63981" checksum="1A582F0DC91BE387" modified="2011-11-16" version="1">MLAQHSPIFPRSRLTISPERSGRNFAQSITSTTLLPVLPDQPTAKSSSIEVYIIPTEKNLFVQGFNAHEYTSQPPTLLRGSLYLRVLKQCKIKSITLTLRGIQKTFWPDGIPPLRHSHWQSNDLITHSWPFYRSETSTCISDNGADCFVELNSKDETAHELPLHPNNSNLAEVFYPGDYIYNFEHPLPASIPETCDSEFGRTSYWLEVVVSRHRVFNPNLSGQIPITIVRTPLVENVRDSESVVITDDWKDQLRFEIVIGSKTFSLDSYLPLVFRFVPLFGKPAVHMIRVYLTQTMKYRYPPKNAKRSDPITKTMLLKYKAKHGHSLLSDTSNDYNYLPRELEFQMYVPQQFESSSYSKIHPDTSYENIQVKHTLRICLRLSTLDPINPGKRKYFEACMEAPVHVLSPLATSRHTLLPAYNDLVPPLVMDNIPHWIHERSGSSSSSIFEQTTSDNQVHTEAQETLGFHHLTTSEDSIGFERTTHLDANLYSPTKSSTLQSHISSPYMSTFEAPLGIPFRAVHLLRNPSFNPPPFDADISPPGIDYLPPPAYDGSYFEQQLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKY5</accession>
    <name>G3AKY5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin-related protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60349</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374543.1">
        <property type="nucleotide sequence ID" value="XM_007374481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKY5"/>
    <dbReference type="FunCoup" id="G3AKY5">
        <property type="interactions" value="745"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKY5"/>
    <dbReference type="GeneID" id="18873357"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60349"/>
    <dbReference type="eggNOG" id="KOG0678">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027965_3_0_1"/>
    <dbReference type="InParanoid" id="G3AKY5"/>
    <dbReference type="OMA" id="GIHYPIR"/>
    <dbReference type="OrthoDB" id="421448at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10221">
        <property type="entry name" value="ASKHA_NBD_Arp3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000029">
        <property type="entry name" value="Actin-related protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="421" mass="46929" checksum="FA70719B0F0FDCE1" modified="2011-11-16" version="1">MSSIGTPAVVMDNGTGYTKLGFAGNDAPSFVFPTAISTSTASSKTSKGGPRSTNLASKRGLEDLDFYIGDEALEAANGPNYSLSYPIKHGQIEDWDQMERFWESSIFKYLRCEPEDHYFLLTEPPLNPPENRESTAEIMFESFNCAGLYIAVQAVLALAASWTSPKVQDRSLSGTVIDSGDGVTHVIPVAEGYVIGTAIKNIPLAGRDISSFIQQLLRDRGEADTSLQTAEKIKEQFCYVCPDIVQEFSKFDHYPKEKFAQYIVEYTDRNKKNIVDVGYERFLAPEIFFNPEICSSDFLTPLPTVVDQVIQASPIDVRKKLYKNIVLSGGSTMFKDFGKRLQRDLKVLVNERIHLSERSSGAQSTGVEVQVISHKKQRNAVWFGGSLLAQTAEFKSYCYTKRDYDEQGPNIVRNFSLFAVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALB2</accession>
    <name>G3ALB2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_150860</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374670.1">
        <property type="nucleotide sequence ID" value="XM_007374608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALB2"/>
    <dbReference type="FunCoup" id="G3ALB2">
        <property type="interactions" value="211"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALB2"/>
    <dbReference type="GeneID" id="18870883"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_150860"/>
    <dbReference type="eggNOG" id="KOG0583">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005687_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALB2"/>
    <dbReference type="OMA" id="AQFNDFY"/>
    <dbReference type="OrthoDB" id="942095at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14003">
        <property type="entry name" value="STKc_AMPK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000650">
        <property type="entry name" value="Serine/threonine-protein kinase ppk16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="38"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="434"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="569"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="685"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="789"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="872"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="438"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="456"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="576"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="597"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="659"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="685"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="708"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="752"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="878"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW33155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="931" mass="105492" checksum="40512FA804F92E75" modified="2011-11-16" version="1">MNGTRLVNDQNQTAQLAAQFNQFYLKTTSAKISQIGNYRIIQEIGEGSFGKVYLARHILLNINVVLKCGLIDDPNIVREIYYHKQLKHKNIVSLYEVIKTENHLWIVMEYCEGGELYYYIYEKKRLTMAECRVIFFQIVLGVKHVHSLNLSHRDLKLENILLADKKRTVVKLTDFGFIREFNPYSRKFLSTICGTTVYMAPELLKNEKYSGFSVDIWSLGVILYTMLYGVLPFDDDDELKIRAKIIQGEPSYADCVPEEANQLIKKMLSKDPNNRPSLTEILNSGLLMDCHNKYLAENTKRTYTSSSGGDTESIMSINQHYNVMSEPFESRIERDILKKLQRLNFDVDELQASVINNEMNSLTAFYELLLTQEYSKKKRKYMRDKKRKYYEATLRKSRKRVKSVLALTDQVPAQPLERIISTLSLVSNRSKTNLSKINESRKSMDLDKRMDPPSPKTTTTSLSLPATPLTSTPLKSQYKSNGQATFSLSQSPKAAAKSLSTDLGSSSLIDAPSMHRSVSFVPEERRLSSVSSHRESLNIIKTQSNQSSHHHKGGKILNKLQFWKKHKDIEESPVNGKHEKVTSEKSEYSPGMFKKNTSPEHHNQQHERKPSISVLDTADPLSPPLVIDPNFEDHPPASPSDITAPKTPPNVDSRPLTRTRPSSMISQVSQISRLSQMSTMLSESELDILDETDTIDDDYDDDDEAYESSLNDATRMSSVGMTPTSSLVNNKPTVGASTSSGQHKNRPGYKRTPSSDMSVQTTSTTSGGLITPPALHKRQSFPEFHNSLEDLSTERSSSLEAPAPVSYTDTPMHNPVPRPISPDPSKKRRMRLNQVMNGTSNDQPLQHPAYFRTPSPPLGFKFNAKSTRDQSKIPIGKRNSNQPFFQNSVTPKGYQETRQVEDWIHSSNFHVVVKQPTYEPVIDEEEETEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALF7</accession>
    <name>G3ALF7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_60531</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374715.1">
        <property type="nucleotide sequence ID" value="XM_007374653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALF7"/>
    <dbReference type="GeneID" id="18873452"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60531"/>
    <dbReference type="eggNOG" id="ENOG502S20Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112963_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALF7"/>
    <dbReference type="OMA" id="EIDNAMA"/>
    <dbReference type="OrthoDB" id="3997736at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="65"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="222" mass="25068" checksum="95E3D514052F963F" modified="2011-11-16" version="1">MVRPSQIIRLNLSKRLRSAIPPPAYFANPQDIPHPYQVDNGKSNKDLVVEAGSQSVPAQAPSAIQYNNSSSYNNASSQQSYPHSNDGHFREIASLLAMVVLSYIAVDNYTERTKLEKLHNQTTAINLKALQIQQAAHNNDRKTRDLQMLQERKEMAKRTFKMGLHIAILRKQLLEAGIDPINLDEAVREFEKSVRADNSSKNVSDQYLWLDDSSGKYKVSTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALV0</accession>
    <name>G3ALV0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N-acetyltransferase HPA3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_60067</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374224.1">
        <property type="nucleotide sequence ID" value="XM_007374162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALV0"/>
    <dbReference type="FunCoup" id="G3ALV0">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALV0"/>
    <dbReference type="GeneID" id="18873206"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60067"/>
    <dbReference type="eggNOG" id="KOG3216">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_32_0_1"/>
    <dbReference type="InParanoid" id="G3ALV0"/>
    <dbReference type="OMA" id="DGHIRYR"/>
    <dbReference type="OrthoDB" id="7305308at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051016">
        <property type="entry name" value="Diverse_Substrate_AcTransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10545">
        <property type="entry name" value="DIAMINE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10545:SF29">
        <property type="entry name" value="GH14572P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="3"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="154" mass="17852" checksum="1D61BFE7D66DEAB1" modified="2011-11-16" version="1">MPVILRPIEANDKERWIELWTGPGGYIEFYKSLDKITPEISNETFRRFLDPSEPVYSVVAIDDSTGTIIGFANYLTHRNTWTIENSLYLNDLFVSADNRLRGVGRKLIEYIYGETDRLNCKACYWATQFGNHRAQLLYTKVGVKSGFLLYNRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALY4</accession>
    <name>G3ALY4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">THO complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_50228</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the THOC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374852.1">
        <property type="nucleotide sequence ID" value="XM_007374790.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3ALY4">
        <property type="interactions" value="626"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALY4"/>
    <dbReference type="GeneID" id="18871419"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50228"/>
    <dbReference type="eggNOG" id="KOG1874">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003123_1_0_1"/>
    <dbReference type="InParanoid" id="G3ALY4"/>
    <dbReference type="OMA" id="QERWTCI"/>
    <dbReference type="OrthoDB" id="29024at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040007">
        <property type="entry name" value="Tho2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021418">
        <property type="entry name" value="THO_THOC2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021726">
        <property type="entry name" value="THO_THOC2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032302">
        <property type="entry name" value="THOC2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21597:SF0">
        <property type="entry name" value="THO COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21597">
        <property type="entry name" value="THO2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11262">
        <property type="entry name" value="Tho2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11732">
        <property type="entry name" value="Thoc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16134">
        <property type="entry name" value="THOC2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THO complex subunit 2 N-terminal" evidence="8">
        <location>
            <begin position="9"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunitTHOC2 N-terminal" evidence="7">
        <location>
            <begin position="624"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunitTHOC2 C-terminal" evidence="6">
        <location>
            <begin position="913"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="331"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1276"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="339"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1276"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1315"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1397"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1431"/>
            <end position="1449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1450"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1481"/>
            <end position="1494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1539"/>
            <end position="1551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1552"/>
            <end position="1575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1576"/>
            <end position="1592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1621"/>
            <end position="1654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW33337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1654" mass="188860" checksum="5E31A3ECA8E9158C" modified="2011-11-16" version="1">MSGELSTYFTDETINEFQEGGIEPLMEVLESYEESNQDSEDAVALLFTELILSFEDNKLELEHIVQFLSQSITNDELARIFCQVLNAFPATENLQKLLVLISRKENVIKPETLALHIGSDFLKESEIVPKAVYTKALNSRIRDEFYTQKKFNLLHEETEGYAKFFVEIYTIFRNDGTEFKIDYALQIIEKLIGHYSLDPNRCLDLLLHIFDSVFVPKVSEALQLIRKSRWWPSVESDNTSISTLSKGGNENGAKVLGLRLMKLPAGHELPEGFKILITCLIKEGFISFGSMYKYMSPNEEAMNELEKDYQKRLEEEVSKAGANALALAAPLADDEDEGRDQNGGKGDQKSTKSETKPKKSIEEKLVTNYKFQFLKTFLSNGLYMPSLFLLTKYPFLAQIDPEIPVLLNRLFDALIQPLYEQVRVFSSEDLQGLQEPKQIAFVRPHNGVYYEAATTTNLLTFKPTLKSYYHKKFHYFYEAWNTKLPIINDIESFITVSKEFLKFNGVNLAQNTQAFIKVCEIVLWDLKNNISDEARKVEWFHYFRNFIFPAMCVIEENSIAIDKAYAILSLYPIEDRFSVYGELYSVLAKNNPLIKMAYNKAEKSTKDVLKRLSKENVRPMMRRLAKISFSNPLPCLLTILQQIESYDNLNTLVVETARYFSSYGWDNLTVAILMRLTAAGRNTTHDDGMVERQWIQSLASFIGKICQRYPRAIDIKTILSFLLKSFHSGDTIGLLVLKEMFISMGGIQLITNLTLHQIDMINCGESLQKIVYRTIDDLRYERFASGKHLVRCFLELDAINELLVLLVHITNEVIYESDKIHLKVLASRSDDLTTVIRLFNTLVGFFGDSEEIKDKLLPLDELYTKYGVPMEWAFELWRPTISKTLSNDDETFSSSLETIKDQIADLIPKNSLEYLSPGLVVCFWQLSLKDVNYSRSLYETETSKLESNIRSLRESIAMNSRDKDVARATIDRQRKELYENEQYIKVLPSEMNAHYEHDKLITAKLQAECASWFLSDDPGKTAIQIEQFLQCCVLPRCIHSSFDAVFVARFIFKLHELKTENYSLIALLDQLTKNKILFGTLFTLTPTEAENLGLFFADVLRTLQGWTKENRFQENEDLLDAAGDVISFDDFRVLLYGYHTTILEEIEASLGVTEYMSRRNAITFLKNLLGVYPTVEDHCERVVRIIEKISLTEEREDLKLSSGALVGHVKSRAKTWLKIWEFIPMSEEEKEKLIKEKQEAQRKEIEKKLEEKRRLLELKKEEEKLAKEEELKKRTSAINYDSQKSSSRPDTRGAASSSRGRYDNYSRYAPTPKEMSVEAAEKESDVESKVNKNESEDRKSSGDHKDAQPEISKIEKEEESKDSQSTKKNPQEDDLIIPRKDSGRAKSRDFQRGSAPTGPSSQQSDSVNPPEKSQDETRATGGDLKSRLSQVKREFQQRTSKESTSEQQDKSQPQTASTVKTEGYTDQEESQTKKRAPLPPQQSIRLQSSAPQQESHTHSRVPLPPQDTLKTRSAAPSAPVSRFSDSRGGRGTPRHHHTYNNQRQPQSNYNRASGSSSTGSNAAASSASGLTAATTPLPPPPPPPPPPPPPPVSTKRNETYNSGQFRNGRESYGRSDSYNNRNHDGKDSRQGGRYDNKRKQDGYGGRGYDKKSRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM01</accession>
    <name>G3AM01_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_71209</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374869.1">
        <property type="nucleotide sequence ID" value="XM_007374807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM01"/>
    <dbReference type="STRING" id="619300.G3AM01"/>
    <dbReference type="GeneID" id="18875365"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_71209"/>
    <dbReference type="eggNOG" id="ENOG502QSXK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008372_1_0_1"/>
    <dbReference type="InParanoid" id="G3AM01"/>
    <dbReference type="OMA" id="TFGNSWG"/>
    <dbReference type="OrthoDB" id="1612078at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021102">
        <property type="entry name" value="PNGase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056948">
        <property type="entry name" value="PNGaseA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31104">
        <property type="entry name" value="PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL)ASPARAGINE AMIDASE A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12222">
        <property type="entry name" value="PNGaseA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A N-terminal" evidence="2">
        <location>
            <begin position="108"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="692" mass="77565" checksum="873182D012FB5544" modified="2011-11-16" version="1">MSHRARDYSIWNMLHYGRAPDAELESETQPETSTTTTIRYSEDKEDIPPQYIEEKEGLLNEYKDEKSGYTSAVQDLEKGEPSKPRNTPTQEKRGSYSGPKHIIEVSNPNIPPKHYGSSVHSAVLIEHTFGHSWGSPAIQKFTPPDVDYDRVVLTLHTEVEGVQYDRLANLFVNGVQIWRTSTIEPGGRKVESDFKKDVSTYLNLFKSEADVMFQLDNILTPGLTGEFKILFTVDLYKNKKWRKFAKERHLFSIKKPADAIYPLTVNEDSKVPPVLYIPNDKLRVNLPSVPKNTTRLVLSIFTSGNAAEEFWYTNVLDKYRDKFQDHGNQFIGRGPIRVVNVFFNGEKIAVQTPQPVIFTGGISPALWSPVVSIDAFDVPSIDVDVTGLLPLLWESDKEKFLEIEISNGLGETGHDDSANVNTNWITTANLLGFQSKHVVDANGEVQNIDHSSRAHVVAASIPFTHSYQQIISSTFKAQLISKLSFILKNGTSLNTTISSYTQAKVDNVQSYLHYGNTQRLVHSGASQKSVLIQNDDIPLSDVNDVDDDSKHGHKHKLPSNTIHSVNVTQLYPLVLSLNEKKRESQPDNSFELEFDVKIVQAKAVDVAFHGHKVLNATIGQNGTSTYVLSSKGNHGFGSLRTTLQATYGPQKFAHNYKRVVDTANGTIVSDETENFIDKSLDDQERKYSRLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM36</accession>
    <name>G3AM36_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="5">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_149886</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="5"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374256.1">
        <property type="nucleotide sequence ID" value="XM_007374194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM36"/>
    <dbReference type="FunCoup" id="G3AM36">
        <property type="interactions" value="506"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM36"/>
    <dbReference type="MEROPS" id="C19.087"/>
    <dbReference type="GeneID" id="18870847"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_149886"/>
    <dbReference type="eggNOG" id="KOG1867">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008279_11_2_1"/>
    <dbReference type="InParanoid" id="G3AM36"/>
    <dbReference type="OMA" id="NVSCNCI"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF937">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="8">
        <location>
            <begin position="91"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="7">
        <location>
            <begin position="217"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="547" mass="61838" checksum="9EFA1F99E7EA20F0" modified="2011-11-16" version="1">MSKKLVPASSSNGGEMYDDDDSFEEEQHTTNVNGNGNENGFHIPPTASPVPDNYSSIVACPHIKSVLESRAKETVFVTYRQAVTISQPLNPTQTFISNKDGQEVSFDKLLAKQVASLKCTDCHLNHFQNSMICLQCPHVGCFGSHNHAMLHYKSTQHLFAIDSRSGLLYCFSCGNYTNHSALEEIRSEIMGNGGTNNVVYNEENYSDPNEVSINALKGFVNLGATCFMSSILQTLIHNPIVKYQFFNNDLHYFNCERNRLQYMNGGVIEENNACITCSVDSIFQYFFSNSSTEGFGMTNLLTTSWYKKKSLAGFQEQDAHEFWQFLLNEFHSDLQRVMGRPSLPALNDNNQCKCITHTTFSFELQSSIKCASCDQITETIDPMIDLSLEIKNSQNKQIDIYDCLDLFTSEEKLDILYTCNFCGDKTKPTKRLRLKSIPPVLSIQLKRFEHKNDSTSKIETPVSIPLFLDLTKYCDGENTDSIDGNKVFELFAVVCHIGSVNTGHYIVYIKDGNGRWFMYDDSVISLVSQQDVTNKNAYLLYYITHKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM52</accession>
    <name>G3AM52_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_149927</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374272.1">
        <property type="nucleotide sequence ID" value="XM_007374210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM52"/>
    <dbReference type="STRING" id="619300.G3AM52"/>
    <dbReference type="GeneID" id="18870849"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_149927"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040688_0_0_1"/>
    <dbReference type="InParanoid" id="G3AM52"/>
    <dbReference type="OMA" id="SVPANIC"/>
    <dbReference type="OrthoDB" id="8922241at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF120">
        <property type="entry name" value="KRUPPEL-LIKE FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="230"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="258"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="157"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="197"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="300"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="305"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="336"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW32757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="347" mass="38912" checksum="2C26F4FAB72E8F76" modified="2011-11-16" version="1">MRSMGIFANPTTPAYSSSTLQPGPILGSSPPTKRPRSESSYTSPQPNMSYTRGSGNDHQSQQQQQQQQQSSEPENEDQRFLRLARDALVATAQGVSGQNNQLVDPTISDLLTRLQYASSPHGNPISQSEKIRADNEGKLMIQGFYSQFPNLSNDIFTGSPNRPKEDNYGHQPQSVHNPSGDEEGWNFLIGDSTRTSTLMEQTSSGSRSQSISISRKSSTTGDSDNPARKFLCDKCSMSFRRSSDLKRHEKQHLSIPPNICELCGKGFARKDALKRHMGTLTCKRNSEKKLYIDNLNYLGKRGGQIEEEEDDDDDNEEEVDNEEDRREGSSNQFSRYDPHNPRSDWPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM59</accession>
    <name>G3AM59_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60113</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374279.1">
        <property type="nucleotide sequence ID" value="XM_007374217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM59"/>
    <dbReference type="STRING" id="619300.G3AM59"/>
    <dbReference type="GeneID" id="18873224"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60113"/>
    <dbReference type="eggNOG" id="ENOG502RQ18">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_990446_0_0_1"/>
    <dbReference type="InParanoid" id="G3AM59"/>
    <dbReference type="OMA" id="ADSEDIY"/>
    <dbReference type="OrthoDB" id="4093337at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442254" evidence="1">
        <location>
            <begin position="19"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="276" mass="29974" checksum="A986E8A959088EA7" modified="2011-11-16" version="1">MKLFYSLLSLFFATFAIGAAVQGDAFQQEFNEFLAVLESYNDPSVFKRDYPPLTAALTALNKSGQGVQIAHAMYSNKATQPTVINAVENYINATTLTQILKDADDSNLAVDIVMILFTNPNMIPGLTNIIKTLHDDGVFSFSLKKRGLLDGILGGLNGVITSIQNQSIQTIVKLMNSVADSEDIYESLEKSGLFISVFEDIVTTTDGQAFAVNLVTDIINDDVITWSSLISAVNDSAIIASTASKIFTNATNRSIILQWILTNGLSVLLEIIEALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMP0</accession>
    <name>G3AMP0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PH-response regulator protein palH/RIM21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_60831</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374999.1">
        <property type="nucleotide sequence ID" value="XM_007374937.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMP0"/>
    <dbReference type="GeneID" id="18873594"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60831"/>
    <dbReference type="eggNOG" id="ENOG502RPPB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029470_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMP0"/>
    <dbReference type="OMA" id="WEWIFNI"/>
    <dbReference type="OrthoDB" id="4079240at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071467">
        <property type="term" value="P:cellular response to pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014844">
        <property type="entry name" value="PalH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35779">
        <property type="entry name" value="PH-RESPONSE REGULATOR PROTEIN PALH/RIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35779:SF2">
        <property type="entry name" value="PROTEIN DFG16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08733">
        <property type="entry name" value="PalH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="237"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="310"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="240"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="273"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="331"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="356"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="366" mass="41705" checksum="A5FFA29A9189CD2A" modified="2011-11-16" version="1">MVVLSRLFTAIAWFQIALKISRQKYKWLTMTIGGVLITAHIVAQIYYQIVFDNSAAIFGDNLVGAFQHWKIARACLQLVILIWFAANILCYTTVIKSPRKVCYSRRLLPLAIFNWCLIVLNATLTILYVSLFRYNWLVKTWLGLLPYLLDVILITTVWEWIYKIWVLEKRFELMGVLGRKMSADEGMSINSDDIGRNKMVLQSKNKVLSMLDGILHIGNNKIVEDVGSVKSIELEDLSTKDSSMPSDQTPQSNEHSHQHLHHRVNFFVDDETPGTSPSNANVSPNTNPDGSDADYDDEIVDNYEIWGREEDDTDEIGNHEIGDDLHEVIVGSSNQFDSGSNDSTEPPPFQPLPGFSEEDYWNDEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMV6</accession>
    <name>G3AMV6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Protein transport protein SEC24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_138597</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SEC23/SEC24 family. SEC24 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375646.1">
        <property type="nucleotide sequence ID" value="XM_007375584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMV6"/>
    <dbReference type="FunCoup" id="G3AMV6">
        <property type="interactions" value="884"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMV6"/>
    <dbReference type="GeneID" id="18870131"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138597"/>
    <dbReference type="eggNOG" id="KOG1985">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004589_2_1_1"/>
    <dbReference type="InParanoid" id="G3AMV6"/>
    <dbReference type="OMA" id="AVECSKQ"/>
    <dbReference type="OrthoDB" id="49016at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990753">
        <property type="term" value="C:equatorial cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050550">
        <property type="entry name" value="SEC23_SEC24_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803">
        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803:SF39">
        <property type="entry name" value="SECRETORY 24AB, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="15">
        <location>
            <begin position="230"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="16">
        <location>
            <begin position="304"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="18">
        <location>
            <begin position="550"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="17">
        <location>
            <begin position="643"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="14">
        <location>
            <begin position="782"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="8"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="76"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW32370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="928" mass="102031" checksum="A5748844EC0468BC" modified="2011-11-16" version="1">MSGRRRAYPQPQYAAGQPAAMASPQMNQFQQPQQQQQQYDPPAAYGIDQAQQQFQQMNIGGGGAAAPGIQSPNSGAAQVNQFQYQQQYQQPQQSPAGQTPQLGYDPYGAQPQASTGYGSAYGSVGGQAGGSAAIPLNQLYTTDLSRELPPPISDLSLPPPPLVLPANSTVIPNSETANANPDYFRTTLNVVPTNSGLLKKSKLPLALIVKPYNALKIDVENIPVTCDTTISRCRRCRGYINPFITFAENGRRWRCNFCNLLNDIPAAFEYDEISGTSKNKYDRVELNHSVVEFIAPKEYMARAPQPVVYTFIIDVSVHAVQSGLTGTITRTILESLDRIPNQNKTAKVAFIGVDSNLHYFRFNEGLDGTELMIVSDIEEPFLPSPSGLLVNLDENRQAIEKLLLDFPTYFEDTANQGFALGPALKAGHKMISNIGGKLVCFGATLPNIGEGKLSVRDEVGVSGKAKEAKALLTAADSFYKSFAVTCNSCQITVDLFLTSSAYQDVATLSNLPRFTAGQTHFYPAWTSAKFEDVTKLSKEVGDHLSQDIALEAVLRVRGSTGVRMTSFYGNFFNRSSDLCSFPTFPRDQSYCIEMSIEENLTKPVIYFQAAVLHSTCFGERRIRVMNLAVPTSPRLNDVYASADQLAITNYYTHKAIEKALSSSLPEAREYLISRVVDVLNVYKKELVAGNVSGASPLQISTNLRMLPLLLFTLTKHLGFRADRVPSDHRAAALNNLGSLPIPELIKSIYPSVYSLHNMPDEAGLPEVITTEDGQEQAVGDIVLPDPINDSKASWENYGLYLIDNGAELFLWVSGNVVPGLVQDLFGTDNLYAIPTGKTELPEFSFEESEFNFRVRAILNKIRDNKDSIVWKNLYVVVGGSSNEPIEISQQRDLMALRMWASSCLVEDKSATEPSYRDFLTSLKTKVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMW5</accession>
    <name>G3AMW5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_137949</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375655.1">
        <property type="nucleotide sequence ID" value="XM_007375593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMW5"/>
    <dbReference type="FunCoup" id="G3AMW5">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMW5"/>
    <dbReference type="GeneID" id="18870085"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_137949"/>
    <dbReference type="eggNOG" id="ENOG502QW5G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014489_1_0_1"/>
    <dbReference type="InParanoid" id="G3AMW5"/>
    <dbReference type="OMA" id="WFADFEI"/>
    <dbReference type="OrthoDB" id="424974at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF49">
        <property type="entry name" value="LYSINE BIOSYNTHESIS REGULATORY PROTEIN LYS14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="18"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW32379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="672" mass="75937" checksum="2A9F414C892C0311" modified="2011-11-16" version="1">MGSDKVKKENKRGYSRGGCRECKRRKIKCDEQKPSCSQCSRLQKQCSYPKEGEKVLRVSKKFLESNPNPVVESNPKPFTIQMYSGPDDFGKGAKKRKIMKKTKMVHTSDNGDAPPKQSKSSSSIFNLLNTTSGTSPLQQTGTDVNAVITGSATSMVRGSPSSSVLDYLYNDDDLNLIAADLNNIVSDIMFTSNMNIENFQSSIENDPSFLFNPALIPEETSIPETTHYDNAIPKNIPLDYIKLNTDDERRYLKDFYDTFASQILPFGAYDPITTRYTNPLRDVILKYSYKEPFLLSAVLSQGAKISSEKTNSKKDIDAYGSYLSTCLKLLGPALSRNRDKKVKDDLTSNVESILITVLLLTSSNASTAKQSWRPHLKGAKDIIMKATHSKMGSSKALILCKIWFADFEILAGQSSHLGGTLKLDEELDSVINFTDPYEHKVLQEFGMIQPNTYNIMFGYNTDCVYLFRDLSKILNKRRIEGDEFVPNDSLEYIRLISGFYEQYNRVYIDRNCTMSYPPHPLLNLTDTVSTEIGPIHISWMDITQQAYSLAGLITIFTQVFMDPPDLPHIKDLNDKLVSLITCFEKSKDHMKMRFPYGFSMIQWPISVAGVNCTENPQKPLIAKYFEICAKLGSSSAEIALKRIKKLWELRERGEEFPELDEDESTKIDSVAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN18</accession>
    <name>G3AN18_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_61503</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375708.1">
        <property type="nucleotide sequence ID" value="XM_007375646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN18"/>
    <dbReference type="GeneID" id="18873921"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61503"/>
    <dbReference type="HOGENOM" id="CLU_1441881_0_0_1"/>
    <dbReference type="InParanoid" id="G3AN18"/>
    <dbReference type="OrthoDB" id="4011368at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="188" mass="21796" checksum="1863B800B45A1054" modified="2011-11-16" version="1">MVETTIEYPQLSHKIDIQSAARNITAPVPDVELSNKNNLKDIYDIYTRVIRFNLGLHMSSLASNFTGAVLSGTSGIDQFVEFLHHTPDEVEKVVYEIRHNDWIRSIPNLRPRLIKLVKTSYESPLILSLLQSNDWLDESRMDDDFRWALSHKISQEFSCKIDEYDVKVCVFSLALNLWINELEFQLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN20</accession>
    <name>G3AN20_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_139290</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375711.1">
        <property type="nucleotide sequence ID" value="XM_007375649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN20"/>
    <dbReference type="STRING" id="619300.G3AN20"/>
    <dbReference type="GeneID" id="18870197"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139290"/>
    <dbReference type="eggNOG" id="ENOG502SDWB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138804_4_1_1"/>
    <dbReference type="InParanoid" id="G3AN20"/>
    <dbReference type="OMA" id="EKHQYAL"/>
    <dbReference type="OrthoDB" id="274726at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009846">
        <property type="entry name" value="SF3b5/RDS3-10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07189">
        <property type="entry name" value="SF3b10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="81" mass="9272" checksum="583B907E132A58C7" modified="2011-11-16" version="1">MADKLREKNQYNLLKSKYGSIGNPDTTDTTEFLTNIHKDTISSLAHHSQLLLYNSIVLNQHPHLLKQELIKKINKTNASKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN29</accession>
    <name>G3AN29_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DUF605-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_61511</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VTA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375719.1">
        <property type="nucleotide sequence ID" value="XM_007375657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AN29"/>
    <dbReference type="FunCoup" id="G3AN29">
        <property type="interactions" value="455"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN29"/>
    <dbReference type="GeneID" id="18873926"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61511"/>
    <dbReference type="eggNOG" id="KOG0917">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_679712_0_0_1"/>
    <dbReference type="InParanoid" id="G3AN29"/>
    <dbReference type="OMA" id="YCKIYVL"/>
    <dbReference type="OrthoDB" id="391137at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.270">
        <property type="entry name" value="Vacuolar protein sorting-associated protein vta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044538">
        <property type="entry name" value="Vta1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039431">
        <property type="entry name" value="Vta1/CALS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023175">
        <property type="entry name" value="Vta1/CALS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041212">
        <property type="entry name" value="Vta1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46009">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VTA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46009:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VTA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04652">
        <property type="entry name" value="Vta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18097">
        <property type="entry name" value="Vta1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vta1/callose synthase N-terminal" evidence="10">
        <location>
            <begin position="16"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Vta1 C-terminal" evidence="11">
        <location>
            <begin position="335"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="204"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW32443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="380" mass="43135" checksum="1CA857247C10575E" modified="2011-11-16" version="1">MINLESIPEELKSDKSITPFIARALELATVNPVVSYYCKFYVLDHILTNKLHTKSKDIESFTIELLDDTEQIKKSTDDENLHKVLQDKNLSITVVLTFAYKLFNSCLESLNGLTRANKAATIQKFRATLTFLQVLSIFNSEEIEWSKLTGGKADSFAEFDGLNKEKIKVIKYHLSRLIKDEVVYTDEPNDEELEQELEDLTKGELDLDEPEEAQEKEQIDKSEEPTPEKDDLVNLDNFDDDKEKDTNEVSLPGVPHIPPQDSDDDQVKLPGAPTYLPDDDITHINKDSSIHVFPPSPPAKHVPTVRKASASSIHTNVSHAPITKETIKQILNRDDTITQVQKHAKFSISALQFEDFDEAEKQLKQGLELLQILKKQEEHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AN47</accession>
    <name>G3AN47_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial group I intron splicing factor CCM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_67073</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375737.1">
        <property type="nucleotide sequence ID" value="XM_007375675.1"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AN47"/>
    <dbReference type="GeneID" id="18875137"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67073"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_272246_0_0_1"/>
    <dbReference type="InParanoid" id="G3AN47"/>
    <dbReference type="OMA" id="IFITSIM"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1315"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1322"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1338" mass="157174" checksum="8F460F76D53DD81E" modified="2011-11-16" version="1" fragment="single">MGLLRNIQSLKLVLRNSTRNFSILPQFKVRFPNQTTYNPLVSHSNLISRGIQIQRCYNTTILKEANANSMPITDYLNHEYSKIRGLLSDNQQRTKYLTHLRAASSFTPEEIQEILQDYQRLVDVLHHECGWDFFREFWHAIFYNGTKSDPSISELRITILESRSALCKLLLRKADYKTYWECIEPLVEQYSSPTLLDSCIQLLQLQADSNGKIQINREEIEQFLEDEENSLELKKDYLIILASTVLKFVAEDMEQKLKYIDTFFELRNMFENNDWIFDPSIDQTYDKVLRIIGKPPSQVEFSTYATKLLEVIKGRYLDDMESITIITRIMGAMSESDPIICMKYFKFKLCLIDEQNLPSNTILSSDDLLYAMIANIHEPRRSYELYIRFPELQSPDHVTILLRISLASNNWFSLQERFAKYYGRQFVVFPTEADKISPAKYGAGELSNENMDHSSNVGITMKGYSNSWLPGGYRRIRKMERYSAANRITRTSLMCSAIIWGLIKRGSNSRALRTYDRYIKSVSFGKSDPSGSEKLLSMFLPLFMQPSDIQNLVTILAHYLRFEQRYNLYLVQPKDIKSCLEFVSKNFSLSSVKKIHELVQQYNKFSPEAYSGLIEAYTYLGQYEVADKTVYQAHKISRIPLSDPGIFVSQLRNHQAWLKRTNDESIVAYNKKMIALLLDLLIQPSIIEKLDDYRDFIPHLIKLNLEYGNEELATTIFESARKRFILTEDDYYPLLEYYVDQNRDCEKFEELLIMMREENITQTARTYSLAVRASRITGYPDVKVIRMLSVYGVTDSGLPYAVLNFKRDAYHICQAVIDFLAYRKIRDITVLNMVVTKMKREFNGTIPFEIRSIIHEFMKYHFKRQPDKLNQLNEIMYKDYRTLTYRFISEYPFEGKRIMVPTLQEQFGNILVSELRTSMKKREEMIGKDNPEKASKGIVGPDRARNVEYAIESAISEQVIGGSTNLNLIILKLLEAPNFEYLTNALDICEKHMIKGSWRFIKEYEAKKLCFKMCICHLGDEYSAEEIESKFKMFLDFYEIESIAQARQDLDTLDPKPKNFNNFMRSSSGRLFDLDDAPVYPYATMNSSQFVEYFHPEQFYKSFETWINPHTTRRLYEEVTRYCNTNAHMWDNFRERFPDTVNYIIKYNSLGTRLSDFRGNFKNITGLHYGGTLDKRIYTTIKEFKRLFEGRQFTIGVDTPPVPKSNIEKDQRMKLKEFKEEFNKINKRLNSIGQDYSQVKTFSDLNLLRFRSRLGRLIRQVDRLEHRIFKSLGLKQQGPYHKIDRSVKFYSRLYPSTQRSRAIEMTRARATTRKKGYYRNHHSVDIKKPTKPDSDETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANF7</accession>
    <name>G3ANF7_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="18">ATP-dependent helicase NAM7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_138745</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">RNA-dependent helicase required for nonsense-mediated decay (NMD) of aberrant mRNAs containing premature stop codons and modulates the expression level of normal mRNAs. Also capable of unwinding double-stranded DNA and translocating on single-stranded DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW31946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375222.1">
        <property type="nucleotide sequence ID" value="XM_007375160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANF7"/>
    <dbReference type="FunCoup" id="G3ANF7">
        <property type="interactions" value="1241"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANF7"/>
    <dbReference type="GeneID" id="18870146"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_138745"/>
    <dbReference type="eggNOG" id="KOG1802">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001666_4_1_1"/>
    <dbReference type="InParanoid" id="G3ANF7"/>
    <dbReference type="OMA" id="QYMQMNG"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036121">
        <property type="term" value="F:double-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043024">
        <property type="term" value="F:ribosomal small subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070478">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006449">
        <property type="term" value="P:regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd21407">
        <property type="entry name" value="1B_UPF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18039">
        <property type="entry name" value="DEXXQc_UPF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21400">
        <property type="entry name" value="ZBD_UPF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000097">
        <property type="entry name" value="Regulator of nonsense transcripts 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040812">
        <property type="entry name" value="UPF1_1B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018999">
        <property type="entry name" value="UPF1_CH/ZBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF364">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18141">
        <property type="entry name" value="UPF1_1B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09416">
        <property type="entry name" value="UPF1_Zn_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51997">
        <property type="entry name" value="UPF1_CH_RICH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 18" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Upf1" evidence="17">
        <location>
            <begin position="84"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="13"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="C3H" evidence="15">
        <location>
            <begin position="92"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="CC/SHH/C" evidence="15">
        <location>
            <begin position="106"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="C4" evidence="15">
        <location>
            <begin position="152"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW31946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1000" mass="112488" checksum="D7030DE79B8F4B04" modified="2011-11-16" version="1">MSNLLFHNFSKEEDEDNFSNVSDNGVVSSESDNETIDQDHIQNGNGINDGEQHSDHESLVADQMFDSDAMNQQLALVEDLDHAHDEEHEHACSYCGIHTPSSVVKCNTCNKWFCNAKTDSSSSHIITHLVMSRHNQVSLHEDSDLGDTTLECYNCGNKNVFMLGFVSAKQESVVVILCRLPCANSKDINWDTSHWQALIEERQFLSWLAPPPPEEALVNAKLITPQQISKLEAQWRLNRDATINDIENTDKQQEEEVLPILMRYNDAFQYQRSFGPLVKLEADYDKNIKESQALEHIQVKWALGLNNRHLASFTLSTYENSDLKVAVGDEIILRYSGFQREPWEGNGYILRLPNKNQEEFTLELNPSKVTPPTDLTTDFTAEFVWKGTSYDRMQQAMKDFATDETSVSSYIYHKLLGHEVVPIEFDTELPQRFSHPALTELNVSQTNAVRTVLQRPLSLIQGPPGTGKTVTSATIIYHLSKLNKEKILVCAPSNVAVDHLAEKLDLLGLKVVRLTAKSREDVDSSVSHLALHNLVNKGAKGEFKKLIKLKNEVGELSVEDSRIYFRNLRNSESKLLDKCDIVCCTCVGAADKRLSQFKFRTVLIDESTQASEPEVLIPIVKGAKQVILVGDHQQLGPVILDRKAADAGLKQSLFERLVFLGHVPIRLEVQYRMHPCLSDFPSNMFYEGSLQNGVTSDDRLIEDATFPWPMVDTPMMFWANYGREELSSSGNSFLNRVEAMNVEKIITRLFKDGVTPDQIGVITPYEGQRAYLVQFMSMNSTLLDKRDQYLDVEITSVDAFQGREKDFIILSCVRANDAQSIGFLSDPRRLNVALTRAKYGLIVLGNPRALCRNRLWNCLLVHFREKGCLVDGPLDNLQLSMVQLNNTVTSANKYSHRPGKQVPKFGTASTLQSMTDFDSASVLSYDNSAINEGSRTRVDAWPTLGNNNASFNKNSSISANFYSKLDKLNGNYDKTGRYNADNIEDDIRSITSSFAAGLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP17</accession>
    <name>G3AP17_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_61143</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375324.1">
        <property type="nucleotide sequence ID" value="XM_007375262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP17"/>
    <dbReference type="FunCoup" id="G3AP17">
        <property type="interactions" value="139"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP17"/>
    <dbReference type="GeneID" id="18873739"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61143"/>
    <dbReference type="eggNOG" id="KOG0586">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025793_1_0_1"/>
    <dbReference type="InParanoid" id="G3AP17"/>
    <dbReference type="OMA" id="RIAMIDW"/>
    <dbReference type="OrthoDB" id="410920at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF68">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATG1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="152"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="105"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="122"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW32048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="501" mass="56895" checksum="8F6F2197472CBEE5" modified="2011-11-16" version="1">MNSDNIYHPISPSAKRHPKDLQIKVPSNGKLGEPFTSTPSPASTSSVSSRFETITHLPTPIINTNSFINYDTTTDSLRETFSQPRMISPPRIVSDYKLSRHHSLNEKGRRFVSNPERANTESPITPTTSPTSPSSSKWYSQRFVAPHLGIEFEFVDELGVGNFSNVILAEGEGKLVAIKIISIPADSKKHVSNFKSFVSRELNVLYHVSYHPCITSLVDYDITLDISNDDVCNFPKDEKEMTVSDQEYENVQVHNQQLIFLNYCPGGNLLHFLLSHKQANYLHDLQYWHLIKRIVCEVILTVAFLHNKNIIHRDIKLENILLLHDSIDQLTDTNPIVNISDFGLSKRLQHPDQLLDTRCGSQDYISPEVLLGAKYNGKLSDSWSIGVLIFSILENRLPFDLPPTTQSPGVSPSVLKRKRSKNSVAHRIATIDWDWHRVNTYLTNTDDLAMEIKKIITQLKSIVDLLLVRKDKRIDVVELIAKPEYAWIRHGVPDDVVEFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP26</accession>
    <name>G3AP26_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_66723</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375333.1">
        <property type="nucleotide sequence ID" value="XM_007375271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP26"/>
    <dbReference type="GeneID" id="18875113"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66723"/>
    <dbReference type="HOGENOM" id="CLU_1166464_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP26"/>
    <dbReference type="OrthoDB" id="4036563at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="82"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="238" mass="27316" checksum="9EAAD7E80DDC8178" modified="2011-11-16" version="1">MVMRVRWSVDLFLSPLRKLLWYSKVTSTVNNDSKTFNQGSFRVIKYEVVPVLSKNNFWLFRNSKKCKIGGRCSLSNSYGTLIINLENANAKLQNQLHANNEKLNQKYKELSKTNAALVTVKELAYLALEKTNQFQDQISEIRCLKLQQGDNQAELVEESNRFRSKLKKLGLALHDLSVKLETQTNMRSENQVKLEKQELITKNLEATIDELSGNLSEEKFTVASLQQKLAQNSDNEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3APD6</accession>
    <name>G3APD6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Coatomer subunit delta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_61194</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 11">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus membrane</location>
            <topology evidence="1 11">Peripheral membrane protein</topology>
            <orientation evidence="1 11">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasmic vesicle</location>
            <location evidence="11">COPI-coated vesicle membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the adaptor complexes medium subunit family. Delta-COP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375389.1">
        <property type="nucleotide sequence ID" value="XM_007375327.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APD6"/>
    <dbReference type="FunCoup" id="G3APD6">
        <property type="interactions" value="1178"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APD6"/>
    <dbReference type="GeneID" id="18873769"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61194"/>
    <dbReference type="eggNOG" id="KOG2635">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019988_3_0_1"/>
    <dbReference type="InParanoid" id="G3APD6"/>
    <dbReference type="OMA" id="VQFRTHP"/>
    <dbReference type="OrthoDB" id="10266042at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090167">
        <property type="term" value="P:Golgi distribution to daughter cells"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09254">
        <property type="entry name" value="AP_delta-COPI_MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14830">
        <property type="entry name" value="Delta_COP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000003">
        <property type="entry name" value="Coatomer subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027059">
        <property type="entry name" value="Coatomer_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10121">
        <property type="entry name" value="COATOMER SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10121:SF0">
        <property type="entry name" value="COATOMER SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 11" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="13">
        <location>
            <begin position="311"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="221"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="275"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="286"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW32113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="570" mass="61886" checksum="D92ADA15A75962F7" modified="2011-11-16" version="1">MVVLAGSICTRGGKPLVSRQFRDISRDRITALLANFPSLISNSSSQHTSVEDDNVRYVYQPLEEFYIVLITNKTSNILQDIDTLHLFASTVSNLVRNVDEREIFDSAFEIIGAFDEIINLGFKENLTLSQVQTFLEMDSHEEKIQEIIERNKELEATEERKRRAKEIQRKESAKKNMESAFGQVNAGGSMGYDMYQQQTPSYQPTYQPAPAVEVSQAAPALAPRPPARGGLQLGKKAGSARQSHAPPAISRGSVSNPTQSAAEPLLAAQPPVFSHHTNIQQPPQIATPGSFSPSASPAPSTGGAGAGKVPNNGILITINEKVSAQLSRDGSIISSEVKGDLQLRINNSALSNAKILCKTGDKKHFKTHPNVDRNLFTSGSVISVKDKSKTFPANDQPLGVLRWRSVGKADNSSLIPIVITAWVNVDGSGVAHVTLEYELTSEFIDSHSATESHVQGVRITVPIVSDDVQLQDGGNDQISYDVSNEGIIFQIGEISIEDPQGSFEFTIPVSEEDDLFPLEVQFDIVNTGVVESDVSLGGVSIIDVVANNEDEASLPFDLHSHVTSENYIVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3APE7</accession>
    <name>G3APE7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_50713</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375400.1">
        <property type="nucleotide sequence ID" value="XM_007375338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APE7"/>
    <dbReference type="STRING" id="619300.G3APE7"/>
    <dbReference type="GeneID" id="18871477"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50713"/>
    <dbReference type="eggNOG" id="ENOG502QUAB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012105_1_0_1"/>
    <dbReference type="InParanoid" id="G3APE7"/>
    <dbReference type="OMA" id="MTENHKW"/>
    <dbReference type="OrthoDB" id="5865767at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37283">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN YHR131C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37283:SF1">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN YHR131C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="353"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="476"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="495"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="607" mass="67442" checksum="9CA418BC0EFD1379" modified="2011-11-16" version="1">MITEVDHQPSPYVYSNTTRPATINIQPLIPISLNQQGKQKQQQQQQQQQQIEHVPEVSPSPSPSSSSSESDHRLSISSGTSSLSASSCDLSLAAELSSSPPSPSPSPVLSITSNDFYSSSQDEYLQLIEPVPVFPPAYDTLPPGGCPKFPVQSFLPSCGPENFAITNVSVRPPAYTPTVYKIGVVARKVECVTPYELSNHKSWKYLLMELNSTQLNFYQIPAIFEASICNYRPNMLDQKISSYLKRINSHFTNQYDLKFHLYCKQMGLLTSKKLVRSYSLQYAKIGLASDYTKKENVLRMRAESEQFLLEFDNPQELVDWNLSISVGMGVSLDVEQRADPKYRTIPRRRRRNQSSGSSSSSSSTSSSASSATAIISSRLRSSSDPFRGRLSKLKEKLSRSKLSKQQAEAEPAAVVTPPVALVSETSVSRSASVPNLAVIPTHVPEASEDVIPSEHEGFEEEDDEYDEEFNQELRHETDGNHSDGEENHHSTGMGYSVSYSSSSDYKWNPPPEKVRSPRRHYRHLFRCIKALNVDDPWLNRSVVIPTETSPLADVIISRTNSVVSLSTVKSTRSRSFSTSSASLVKLPCHYLREYNVGANGLVPTEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>G3APS4</accession>
    <name>G3APS4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_61324</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375521.1">
        <property type="nucleotide sequence ID" value="XM_007375459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APS4"/>
    <dbReference type="GeneID" id="18873842"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61324"/>
    <dbReference type="HOGENOM" id="CLU_2905611_0_0_1"/>
    <dbReference type="InParanoid" id="G3APS4"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="62" mass="7519" checksum="991D24178EFF6662" modified="2011-11-16" version="1">MDVDEFLTAFHNGFKVDSSHFKLIIEVHQIHQNDKSFKNYRQVFYNFLDHHSQKTVEGYQPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3APW1</accession>
    <name>G3APW1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sphingosine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_55773</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375558.1">
        <property type="nucleotide sequence ID" value="XM_007375496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APW1"/>
    <dbReference type="FunCoup" id="G3APW1">
        <property type="interactions" value="610"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3APW1"/>
    <dbReference type="GeneID" id="18871969"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55773"/>
    <dbReference type="eggNOG" id="KOG1116">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013399_0_2_1"/>
    <dbReference type="InParanoid" id="G3APW1"/>
    <dbReference type="OMA" id="QAWSRRI"/>
    <dbReference type="OrthoDB" id="3853857at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001727">
        <property type="term" value="F:lipid kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055916">
        <property type="entry name" value="DUF7493"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050187">
        <property type="entry name" value="Lipid_Phosphate_FormReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045540">
        <property type="entry name" value="YegS/DAGK_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358:SF31">
        <property type="entry name" value="ACYLGLYCEROL KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358">
        <property type="entry name" value="SPHINGOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24321">
        <property type="entry name" value="DUF7493"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19279">
        <property type="entry name" value="YegS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAGKc" evidence="5">
        <location>
            <begin position="120"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW32282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="498" mass="55109" checksum="3F3CAB2DDFAFF61F" modified="2011-11-16" version="1">MSSERLIYARNDLAQVTIDDHGVKISEQSLWAVDHEVPSSNSLFCCGGQPIPIKTFHDHIPFRNIIYVDPGANDDEVTLTYVYPTSKDSIKLATLEVQVQNYNNNKLSEYILDKAYTNHIIRPSVLVLVNPHGGQGKAVSIYENEIYPILKAARAKVTLEETKYQTHATDIAKELDISKYDIIACCSGDGIPHEVINGFFAREDKGAEAFNKIAVTQLPCGSGNALSLSTHGSNNAGMATFQMLKAKRTKLDLMAVTQGSGPAKKTKLSFLTQCYGIIADSDIGTEHLRWMGPVRFQIGIAQKVFSGAKYPCELYVNFLTKDKREIINHVETHMQLSSSQRDEELPVVTADNLAVTGPDLDQPVPDNWAHISAEITDNLNILYVGKMPYISDSAQFFPAALPNDGSMDMVMTDSKASFMETTSIMLSVEEGTHVHNDKVKHAKVLGYRLIPKVNDRTKHYISVDGEEFPFEALQVEVLPGVLTGLLQDGSYVETCFTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ05</accession>
    <name>G3AQ05_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_139500</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375603.1">
        <property type="nucleotide sequence ID" value="XM_007375541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ05"/>
    <dbReference type="FunCoup" id="G3AQ05">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ05"/>
    <dbReference type="GeneID" id="18870214"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_139500"/>
    <dbReference type="eggNOG" id="ENOG502SD6J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128881_1_0_1"/>
    <dbReference type="InParanoid" id="G3AQ05"/>
    <dbReference type="OMA" id="HRVGFEL"/>
    <dbReference type="OrthoDB" id="4002732at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039196">
        <property type="entry name" value="Fmc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28015">
        <property type="entry name" value="ATP SYNTHASE ASSEMBLY FACTOR FMC1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28015:SF1">
        <property type="entry name" value="ATP SYNTHASE ASSEMBLY FACTOR FMC1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13233">
        <property type="entry name" value="Complex1_LYR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="149" mass="17354" checksum="D0B83DF3C7827A81" modified="2011-11-16" version="1">MSIYSKTFPSVKQLTKTLLEELLIIQNKAHQVQAAREREKQIALLSYQKLTGIKQGKDVRELESKLKKLNETTEAVEHKELHDSHILKDYVQQKEMSVHQRQNLHNMVTFLNSQRVYIELLERYNPGLTMSQEDNVRKTANKVGLSVPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ14</accession>
    <name>G3AQ14_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_61412</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375611.1">
        <property type="nucleotide sequence ID" value="XM_007375549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ14"/>
    <dbReference type="GeneID" id="18873880"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61412"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1036500_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQ14"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="166"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="269" mass="29809" checksum="C45DCE96ADF4CFF8" modified="2011-11-16" version="1" fragment="single">MPQFGNGPGKHGRQNSSSIPIDQLTLLSLRTPSVSGPPQQQQQTPQQQQIQQQLAGIAQPSFMFDPVSNTSSPNADDFLQSPTNFSYSQAAAAVAAAAAAIPTIPEERTINPRQLFTKLNTSMSSPSLSTLFTTHNSQQQQQQQQQPQQHQHHQHAHHSQQHSHQQHMQAQLSPQQPQAANFPQALSAQISPTQPTVPYFMPQHTRSVSTPQFDFKVDDEFSQAISSWLNGGWVDGLNPEIVNPVKKNRMYSTVKGRRNSIQPQVHVPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQD0</accession>
    <name>G3AQD0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Acetolactate synthase</fullName>
            <ecNumber evidence="4 12">2.2.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ILV2</name>
        <name evidence="16" type="ORF">SPAPADRAFT_62046</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2 pyruvate + H(+) = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="12">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="4 12"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376255.1">
        <property type="nucleotide sequence ID" value="XM_007376193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQD0"/>
    <dbReference type="FunCoup" id="G3AQD0">
        <property type="interactions" value="610"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQD0"/>
    <dbReference type="GeneID" id="18874166"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62046"/>
    <dbReference type="eggNOG" id="KOG4166">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_1_2_1"/>
    <dbReference type="InParanoid" id="G3AQD0"/>
    <dbReference type="OMA" id="QETDMIG"/>
    <dbReference type="OrthoDB" id="16262at2759"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901705">
        <property type="term" value="P:L-isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02015">
        <property type="entry name" value="TPP_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000008">
        <property type="entry name" value="Acetolactate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000007">
        <property type="entry name" value="Acetolactate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000053">
        <property type="entry name" value="Acetolactate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012846">
        <property type="entry name" value="Acetolactate_synth_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039368">
        <property type="entry name" value="AHAS_TPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00118">
        <property type="entry name" value="acolac_lg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF13">
        <property type="entry name" value="ACETOLACTATE SYNTHASE CATALYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="15">
        <location>
            <begin position="3"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="13">
        <location>
            <begin position="188"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="14">
        <location>
            <begin position="395"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW31477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="586" mass="63611" checksum="B8B63CF0EBAAC50D" modified="2011-11-16" version="1">MLRHNVDTVFGYAGGAILPVFDAIYNSDKFKFVMPRHEQGAGHMAEGYARATGKPGVVVVTSGPGATNVITPMADALMDGVPLVVFTGQVPTTAIGTDAFQEADVIGISRSCTKWNVMVKNVAELPRRINEAFEIATTGRPGPVLVDLPKDVTAAILKESIPINTTLPSNALSQITKKAVSEFTSEAIRRSAAILNKAKKPILYVGAGVLNNEEGPRLVKELAERACIPVTTTLQGLGAFDQRDPKSLDMLGMHGSAAANTAMQQADCIIALGARFDDRVTGNIAKFAPEAKLAALERRGGILHFEISPKNINKVVEATEAIEGDVTSNLKSFIPLVHSVESRPDWFAKVNEWKEKYPYSYQEETPGSLIKPQTLIREISKQAQTYDKEVIVTTGVGQHQMWAAQHFTWTKPRTMITSGGLGTMGFGLPAAIGAQVGKPDAIVIDIDGDASFNMTLMELSSAVQAGAPIKVCVLNNEEQGMVTQWQSLFYEHRYSHTHQKNPNFMKLAEAMGVQGIKVSTQEELVSGVKEFLDAKGPVLMEVIVEKKVPVLPMVPAGKALDDFILWDAEVEKEQNELRKQRTGGKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQQ1</accession>
    <name>G3AQQ1_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62211</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376376.1">
        <property type="nucleotide sequence ID" value="XM_007376314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQQ1"/>
    <dbReference type="STRING" id="619300.G3AQQ1"/>
    <dbReference type="GeneID" id="18874218"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62211"/>
    <dbReference type="eggNOG" id="ENOG502RQ12">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_849982_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQQ1"/>
    <dbReference type="OMA" id="ECNDELF"/>
    <dbReference type="OrthoDB" id="21214at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031511">
        <property type="term" value="C:Mis6-Sim4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025207">
        <property type="entry name" value="Sim4_Fta4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13093">
        <property type="entry name" value="FTA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="327" mass="37856" checksum="79C04E2D9304A6BB" modified="2011-11-16" version="1">MNHSYLYTKRFIDEQVKILNQPLRLTDSLKQILQQQDENADKVITEAQLKKVLVKLNTKIRNRNQVIFRAQIVNQIVQQVLKLEQNNLEIVNEQLTKIHYVLKPILIPDFEHLSNKTQLSKLMELGDLIKELPESQYILVRNTAEEEPDSEEESDEASDENILIQDDQDIVEKKPSQRKQIAQHRREVIQKVQQAENRDENLVERYHDLRANLLKLYESIRYKYDRSNYLKGLKNNIQGSLGISKLKADTHGNDQVYDSDEEGEGDYDINGIQTNLITTSANTNPDNVIAEVNKFRVLTEKLSYKVTTTGITGPELRQVVMDINSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQS2</accession>
    <name>G3AQS2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_155043</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat mio family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376397.1">
        <property type="nucleotide sequence ID" value="XM_007376335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQS2"/>
    <dbReference type="FunCoup" id="G3AQS2">
        <property type="interactions" value="775"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQS2"/>
    <dbReference type="GeneID" id="18871008"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_155043"/>
    <dbReference type="eggNOG" id="KOG1008">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005843_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQS2"/>
    <dbReference type="OMA" id="YEPTGHA"/>
    <dbReference type="OrthoDB" id="341486at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035859">
        <property type="term" value="C:Seh1-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16691">
        <property type="entry name" value="mRING-H2-C3H3C2_Mio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037593">
        <property type="entry name" value="MIOS/Sea4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049092">
        <property type="entry name" value="MIOS_a-sol"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031488">
        <property type="entry name" value="Zn_ribbon_mio"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16453:SF9">
        <property type="entry name" value="GATOR COMPLEX PROTEIN MIOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16453">
        <property type="entry name" value="WD40 DOMAIN-CONTAINING PROTEIN MIO FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21719">
        <property type="entry name" value="MIOS_a-sol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21720">
        <property type="entry name" value="MIOS_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17034">
        <property type="entry name" value="zinc_ribbon_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="MIOS-like alpha-solenoid" evidence="6">
        <location>
            <begin position="511"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="domain" description="GATOR2 complex protein MIO zinc-ribbon like" evidence="5">
        <location>
            <begin position="880"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="443"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF21719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1006" mass="113486" checksum="BB5BB7AACCE0BBEA" modified="2011-11-16" version="1">MSGTIVHALNWDNGYEQQFIAVNPMGDEVILYETNHDNDPTVESNAIVQLSNRSGFENIQCSSYSKIKRGLVSVGSINGTISVFDINSSTSSILNLRPKQNRPCNAISFNHNNLIVAGFDKGRQDNSLQIWNIETYSSTGGNSHIKRPLSTYLPNEAILSATFYPDRESSIVCGSYKFLREIDLRSDQPAFQMATKCTLGLTIDHFQNHLFQSFSEDGSLAIWDRRKLTTTKSKSLSSGNVITETPILQFNKLLSDASSRKISHPCVRYSTVRRGEFAAVFNGDLIRRWHTGRVPADYSLSPMNDTSSYRFSSNETNTLQGLKQQASQLYKPSEDSLFVSMVLDVKTDYERVVSFDYSPDITSHTSTNFVCMRQSGSVFRMPVVESIESMDFNVLNEFSIAGPEGTMTKFIDLTKQQEQTEVANRNLSLARVGGLSLTEDLRNNDGEFSEDVSSTVDDESSDVNNKNSNHRHPHHRQLANQRLSFDGFEEIPLNTFLDSSDIISNDICAIIRKRAHMGYSFDCDFNISLLERLDTMDNQLFLRNTWKWLSLARKSLEKGTMISEGIDLGFQGVLGIWKGVDALDGQSRTDTTREGPITDSWFSQAVRSIVSSKGKKTAAINIPSNSEKKAQRKLGLIVSGWYLADNEFEEKLNGLVALGNWEKAAGWAVFHGNVPKAIEILANSQKERLRLMATAVAGYLAYKNSNVNSPWKDQCRKMASELDDPYLRAIFAFIADDDWWDVLDEHALPLRERLGVALRFLSDKDLTVYLDRMAESVVNKGELEGLILTGITPRGIDLLQSYVDRTSDIQTAALIASYACPRYFTDQRITHWIDCYRNLLNRWGFFSIRAKFDVTRTKLSKTFNGQTTIKAAPKQVFLQCTRCNKNMAKFKNPNPVNNQALMKQFNKMNGGKSITNDIVNCCPHCGAPLPRCAICLLTLGTPIPLEQDERLDENDKIGNKIENRFREWFSYCLTCNHSCHAYHAEEWFSKHYVCPVPDCNCRCNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR15</accession>
    <name>G3AR15_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_62287</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
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    <sequence length="418" mass="46844" checksum="257D03C8D73667E1" modified="2011-11-16" version="1">MTPYQNDKILGDDLEHTVIRTEPDESTAAKILSQDEIAYFLSEITHSELSRFPDLLTNKSLTPAPIPPQSPRKLNPLQLDNLSYSPSLASASSKKTSYSSFAPTPNASYAYNSISPVPMVPAESLMTNSSQYLDDPKELDIESLLQMIEQDEPVVSTSTVKSTIPEIEEETLEDFPQVNLDDDSILTRTVSIGSANLDFGNYSDLDQLLQIKSDPTSALMSRVTSYDYTGTSYGSFSTSPMPKVARRVSCPTALRPSIITHEYSSPTPPVYMSNDRILSMTLPKKTTPRITKRHSEVFICPVCGNTLSRAANLKQHCLSVHGEERPYPCRYPDCDWRFSRPNDRTRHENQHSGVKKFICHGVLPNGKIFGCGKGFKRSDGLGRHFRTETGKKCIQPLVEFQESNRRNRFDRPDDSDIF</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
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                <person name="Zinkel R."/>
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</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Sato T.K."/>
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                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <location>
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        </location>
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    </feature>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1506" mass="170925" checksum="59F8EA8831C428BE" modified="2011-11-16" version="1">MSKRRNSTDSGCDDESIDNQTQESYLGFNEDTSRDVQQLARTLTQESAASVGSSSAALMKYLSTIDEAPGINPYQEEITDPELDPESPQFNAKYWVKNLKALYLSDPDYFKPNKLGVAYRNLRAYGVANDVDYQTTVATGFIKFIVDGLRHLRKEDKSRYFDILKPMDALLKPGEITVVLGRPGAGCSTLLKTIAVNTYGFHVDKESKISYDGLTPSDIEKHYRGDVIYSAETDDHFPHLTVGDTLEFAARLRTPQNRGLGIDRETYAKHMANVYMATYGILKQRDTPVGSEFVRGCSGGERKRVSIAEVSLSGANLQCWDNATRGLDAATALEFIRALKTSSTILDVTPIIAIYQCSQDAYDLFDNVSVLYDGYQIYFGKADKAKEFFTNMGWFCPDRQTTADFLTSLTNPAERIAAPGYEGKLPRTAHEFEQYWKNSPEYKQLVTDIDQYFIDCEALNTKQSYHTSHVARQSNHIPARSPYTVSFWMQVKYIMGRNFLRMRGDPSIPIFTIFVQGIVGLILSSLFFNLDQTTNSMNFRGMALFFAVLFNAFASLLEIMSLFEARKIVEKHKKYALYRPAADALAGIITELPVKFLMSLTFNITFYFMINLRREPGRFFFYWLMGMWLTLVMSHLFRSIGAVATSLAQAMTPAAVLLLAMVIYTGFVITTREMLGWARWINYINPVGYAFESLMVNEFHGRYFECANFVPHGGIYENIPMENRVCTTVGAQPGELMVSGTTFLKLAYNYENVHKWRNLGICIGYAFFFLGIYISLTEFNKGAMQKGEIALFLRSGLKSLKKRKQLLEKTQDIENATNEKVPLAEDLGSSDNKEGDSSELSDLATEKEIFFWRNLTYEIRIKKEDRIILDHVDGWVKPGQITALMGATGAGKTTLLNCLSERLTVGVITDGVRMVNGHSLDSSFQRSIGYVQQQDLHLPASTVREALRFSASLRQSNSIPDKEKDAYVDYIIDLLEMEEYADAMVGVAGSGLNVEQRKRLTIGVELVAKPKLLLFLDEPTSGLDSQTAWSICKLMRKLADHGQAILCTIHQPSALLLTEFDRLLFLQAGGQTVYFGDLGENCQSLIDYFEKYGADPCPKDANPAEWMLQVVGAAPGSHAKDDYFRVWRNSQEYQEVQHELDKMEVELAKLPRVDDPESHLKYAAPWWKQYWLVTIRTIVQDWRTPNYIYSKLFLCLATSLFNGFSFFGGKLSLRGLQNQMFAIFLFFVPFNTMLEQLLPYYMSQRDVYEVRERPSRTFSWFAFFAGQLSSEIPYQIVVGTLSFFCWYYPSGLYKNAQPTGQSEARGAMMWLLIVSFYCYTTTMAHLCISFIEKQENGANLAHLLFQMCLMFCGVLAGPDQMPGFWIFMYRANPFTYITQAILAVGLANTNVVCAPQEYVSINPPANYTCDQFMGPYISKAGGYVLTDENGQCQFCQMDSTNEFLATVGSYFHQRWRNYGIFVAFIGINVILSIFLYWLARVPKGNRERKSKRKERIQKLVSSLKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASL0</accession>
    <name>G3ASL0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Signal peptidase complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_51908</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Enhances the enzymatic activity of SPC and facilitates the interactions between different components of the translocation site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPCS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376729.1">
        <property type="nucleotide sequence ID" value="XM_007376667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASL0"/>
    <dbReference type="FunCoup" id="G3ASL0">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASL0"/>
    <dbReference type="GeneID" id="18871608"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51908"/>
    <dbReference type="eggNOG" id="ENOG502S2C7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131066_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASL0"/>
    <dbReference type="OMA" id="TKYDPIY"/>
    <dbReference type="OrthoDB" id="29558at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009582">
        <property type="entry name" value="Spc2/SPCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13085">
        <property type="entry name" value="MICROSOMAL SIGNAL PEPTIDASE 25 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13085:SF0">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06703">
        <property type="entry name" value="SPC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="173" mass="19821" checksum="848A239D8C5F6D5F" modified="2011-11-16" version="1">MSSELKKTNLNSVNDLRQATDDQLGQVMQQFGYEESFALIDIKLGLGVATVVIAGLLFLVDKKFTFKESYNITVISIVLYGFCSGLLYLLNFLNKNVKYTGYNKKGRKVTIATWTANKFDPIYHISVTFGDRKQRVVTSFEFTKFFDVIGFFNRDKFIELLGNEFEKVDKKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASQ0</accession>
    <name>G3ASQ0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63040</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377146.1">
        <property type="nucleotide sequence ID" value="XM_007377084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASQ0"/>
    <dbReference type="GeneID" id="18874565"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63040"/>
    <dbReference type="HOGENOM" id="CLU_880451_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASQ0"/>
    <dbReference type="OrthoDB" id="10597407at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="197"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW31113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="316" mass="33600" checksum="860FDA1D67EB8A23" modified="2011-11-16" version="1">MHHGIPAPTPIPVAIPQREIYFVKHDPPSSLDSPASSYTPNLQFRKIWDDSSSNSRPRNASYFDPLASPGAGGAIAVGSGGGNFTIPRNSSVNSSYSTDSKQSYLPPPLATGINRSTSLVSPSTPDPGSVLPMAAPTIQQNQQQPPRSIPNDFHPTATKKPSFYSQTRPSLSDYSTTKSLPSIGSITSINSNASSVFSNKSSISSVTSLPPPPSAPTNSSLNKILTPTPTTRPPSTPLPQDNYSNTTNANNIVPVYPRVRSLSPQSMLTKDNGDSPKQQMGVPQPQKHRSLTSSPLSKEKNSKVSVNSLLDDSKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASS5</accession>
    <name>G3ASS5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">2-dehydropantoate 2-reductase</fullName>
            <ecNumber evidence="2">1.1.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Ketopantoate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_63055</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ketopantoate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.169" evidence="2"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377172.1">
        <property type="nucleotide sequence ID" value="XM_007377110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASS5"/>
    <dbReference type="FunCoup" id="G3ASS5">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASS5"/>
    <dbReference type="GeneID" id="18874573"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63055"/>
    <dbReference type="eggNOG" id="ENOG502QPT5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031468_10_2_1"/>
    <dbReference type="InParanoid" id="G3ASS5"/>
    <dbReference type="OMA" id="KFLVNCC"/>
    <dbReference type="OrthoDB" id="73846at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008677">
        <property type="term" value="F:2-dehydropantoate 2-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003710">
        <property type="entry name" value="ApbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050838">
        <property type="entry name" value="Ketopantoate_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00745">
        <property type="entry name" value="apbA_panE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43765:SF2">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43765">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketopantoate reductase N-terminal" evidence="6">
        <location>
            <begin position="8"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="7">
        <location>
            <begin position="206"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW31139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="358" mass="39454" checksum="4E532D367F247AF2" modified="2011-11-16" version="1">MSKTTANVHILGAGAMGSLLAHDLKQQFPQDFHPTLLVKPGKSLDSNTQTIKLARQIGPNWTTDSVTVDALKPSDFLSSVTKYEKSGHIDNLIISTKAHQTASALLPFIPYISEKSNILIVQNGMGIPSLLKEKFWARNCPNIYEVVATHGAYIDDDGVVRHAAPGKLTIAKFPGENKQDTPAIVQMLLDTPTLNATLVDYEQFLVIQCEKLVVNCCINPLSALFDCFNGELLYGGDQLSSMYKRIINEAITVLYLEYPMLNQITEASIYLNPERLFTHVVSTTEATAKNSSSMRQDVRMLRLTEVDNMNGYISYLGKKHKSGTLGNAMICDLVKMKLAINKGVNQATTDSLLDISLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASW3</accession>
    <name>G3ASW3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RlpA-like protein double-psi beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_62602</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376778.1">
        <property type="nucleotide sequence ID" value="XM_007376716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASW3"/>
    <dbReference type="STRING" id="619300.G3ASW3"/>
    <dbReference type="GeneID" id="18874398"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62602"/>
    <dbReference type="eggNOG" id="ENOG502S6X4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047639_2_1_1"/>
    <dbReference type="InParanoid" id="G3ASW3"/>
    <dbReference type="OMA" id="WETVTDI"/>
    <dbReference type="OrthoDB" id="623670at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22191">
        <property type="entry name" value="DPBB_RlpA_EXP_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051477">
        <property type="entry name" value="Expansin_CellWall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836:SF28">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442599" description="RlpA-like protein double-psi beta-barrel domain-containing protein" evidence="3">
        <location>
            <begin position="19"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="RlpA-like protein double-psi beta-barrel" evidence="4">
        <location>
            <begin position="164"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="45"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="45"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="222" mass="22698" checksum="12DC01B4AA009F54" modified="2011-11-16" version="1">MRLSTVLLSSVLMTAINAAPALHTVFVTAFTTVLVDSKGNTIYPSATEAPAPAPTTQAPAPAPEPTTSSQAPAPAPEPTTSSQAPAPAPEPTTSSSSAPAPAETAKPSAQGSSSQPSGSIHHGQGTYYDTGMGACGVVSSDSDYIIAISHTMFDAALTDGNPNHNPLCGKKIRAFYGGNSVDVTIVDRCVGCAEDDLDFSPAAFKQIADPGLGRIDITWQWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASY1</accession>
    <name>G3ASY1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14">Fructose-bisphosphate aldolase</fullName>
            <shortName evidence="14">FBP aldolase</shortName>
            <ecNumber evidence="5 14">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">FBA1</name>
        <name evidence="15" type="ORF">SPAPADRAFT_62614</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 14">Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13 14">Binds 2 Zn(2+) ions per subunit. One is catalytic and the other provides a structural contribution.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the class II fructose-bisphosphate aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="5 14"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376796.1">
        <property type="nucleotide sequence ID" value="XM_007376734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASY1"/>
    <dbReference type="FunCoup" id="G3ASY1">
        <property type="interactions" value="774"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASY1"/>
    <dbReference type="GeneID" id="18874406"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62614"/>
    <dbReference type="eggNOG" id="KOG4153">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036923_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASY1"/>
    <dbReference type="OMA" id="PRTWGKL"/>
    <dbReference type="OrthoDB" id="35652at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00183"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904408">
        <property type="term" value="F:melatonin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00946">
        <property type="entry name" value="FBP_aldolase_IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000013">
        <property type="entry name" value="Class II fructose-bisphosphate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000771">
        <property type="entry name" value="FBA_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006411">
        <property type="entry name" value="Fruct_bisP_bact"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00167">
        <property type="entry name" value="cbbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01520">
        <property type="entry name" value="FruBisAldo_II_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006628">
        <property type="entry name" value="PRK09197.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30559:SF0">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30559">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE CLASS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01116">
        <property type="entry name" value="F_bP_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001359">
        <property type="entry name" value="F_bP_aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00602">
        <property type="entry name" value="ALDOLASE_CLASS_II_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00806">
        <property type="entry name" value="ALDOLASE_CLASS_II_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 14 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13 14" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="266"/>
            <end position="268"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="287"/>
            <end position="290"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU366023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW30763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="359" mass="39181" checksum="3EA3A452960280BB" modified="2011-11-16" version="1">MSAPAVLSKSGVIYGQDVKDLFDYAQAKGFAIPAINVTSSSTVVAALEAARDNKSPIILQTSQGGAAYFAGKGVSNTDQKASIAGSIAAAHYIRSIAPTYGIPVVLHTDHCAKKLLQWFDGMLEADEEFFAANGTPLFSSHMLDLSEETDDENIATCAKYFKRMAKLNQWLEMEIGITGGEEDGVNNENVDKDALYTSPETVFKVYEALSPISPNFSIAAAFGNVHGVYKPGNVVLRPEILGDHQAYAKKQISSKSDHPLYLVFHGGSGSTQQEFDTAIKNGVVKVNLDTDCQYAYLAGIRDYVLNKKDYLMSQVGNPDGEDKPNKKYFDPRVWVREGEKTMSARIAEALNIFHTKNQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASZ3</accession>
    <name>G3ASZ3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bul1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_142942</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376808.1">
        <property type="nucleotide sequence ID" value="XM_007376746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASZ3"/>
    <dbReference type="FunCoup" id="G3ASZ3">
        <property type="interactions" value="443"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASZ3"/>
    <dbReference type="GeneID" id="18870504"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142942"/>
    <dbReference type="eggNOG" id="ENOG502QSAC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006028_0_0_1"/>
    <dbReference type="InParanoid" id="G3ASZ3"/>
    <dbReference type="OMA" id="FFTFKFP"/>
    <dbReference type="OrthoDB" id="2283785at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039634">
        <property type="entry name" value="Bul1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022794">
        <property type="entry name" value="Bul1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007519">
        <property type="entry name" value="Bul1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904:SF1">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04426">
        <property type="entry name" value="Bul1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04425">
        <property type="entry name" value="Bul1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bul1 N-terminal" evidence="2">
        <location>
            <begin position="78"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="Bul1 C-terminal" evidence="3">
        <location>
            <begin position="774"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="862" mass="97925" checksum="A45CE05031026AE2" modified="2011-11-16" version="1">MGLGSNSNKEKSNDPPDYDTLTSSKSKPKVSSILRGLRSPSPSSSSRAPRKDVPYKPTPQQQRQADLLTTPSTGSIQTLQTVSTSTQTEYFDILPSFHMFQSILKRDDRQFSEDLHSPPPGYDDTTNSSASPLPSPGIDASLSQVNSRLREYTLEQERERRQDDYEEDPGSATGNAHNANVAVLDTYGYSPLDNIDKLKKAPSSPIDIQIYVTKRVPVPNHTNELETRLKEYSSGDFVNGYIIVANTSDKPVEFGLFTVSLEGTIKIIERNVNNLGMSNHKYNRILMKKFLKMYDLNASYGYTYIPNSMGIQYEEFTKDVSDGCQIALPTDRILQPNTKYKKFFTFKFPDKLLDTSCKDSIMTHVLPPPTLGIDRTCFSNRGESIHLNKALGYGFMNVRGTPVLTKDYSFDDVSVSYTIEAKVIDKVNSNQKLSHQEINEAENDSDYVISKNRQYFLRFVPDLKSAFESNTGFIGNHEGIDGKLFHTYLNKETWRAINVINFNIELEIDEKLTKDEVTPDDMKRKHLVNPDSHIHSEIHKLQRKYAVERHYSPTPEEIKYHKKLMIGTRSSSVIFGKKKKMILSSLVRLGESRMYAKVPDKIIPYSSPKLITKYNTESASITPTVSNEGLRPVASTSSNMDELYHRDKQDIIDEIDISLIFASVDAGMKPPIITSIETNLVFWSYSTEFPLPFELGYDFFYTSPSEGHEYDIDNPVEITKNNLQKLKDHVGVYIHFLKENDIYLSRDSFVYLKSIKSLGIKKDTVTDYFKPLTNSTHPDLLNNELGWKPSQTSRGKIEWVKEMKIPLDRMNKNNVTLLPSFQNCLVGRLYCLQVLVKYKGTGNDQNEFADNIVKVDVPILIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT32</accession>
    <name>G3AT32_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62665</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376828.1">
        <property type="nucleotide sequence ID" value="XM_007376766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT32"/>
    <dbReference type="GeneID" id="18874422"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62665"/>
    <dbReference type="HOGENOM" id="CLU_2348023_0_0_1"/>
    <dbReference type="InParanoid" id="G3AT32"/>
    <dbReference type="OrthoDB" id="10551417at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442346" evidence="1">
        <location>
            <begin position="19"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="97" mass="10101" checksum="0AA2D91B52F6DEE1" modified="2011-11-16" version="1">MIFQQYLLFCFYLTAISGAALVADSEHADVGPQDLASSVTRYDPVSAISPLPVASSTSAIPTTESTDSSGKAVSSASISIYSQTIILCCLISTCMVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT38</accession>
    <name>G3AT38_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62669</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376834.1">
        <property type="nucleotide sequence ID" value="XM_007376772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT38"/>
    <dbReference type="STRING" id="619300.G3AT38"/>
    <dbReference type="GeneID" id="18874424"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62669"/>
    <dbReference type="eggNOG" id="KOG0014">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_612761_0_0_1"/>
    <dbReference type="InParanoid" id="G3AT38"/>
    <dbReference type="OrthoDB" id="1898716at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="273"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="410"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="447" mass="48811" checksum="5B62085613886315" modified="2011-11-16" version="1">MDVDVPEDDESEYDSDSPQPKRHKRSYSELVYSTTNKSKNSKIFNSNRPPPPPPPAHISSLSTMPTFSNPKPKTPSLAPINREEAPSQRPILRVQIPTDAKGHKRTNSNTASNNNMSSSEKSGDTARTITAIDTNLQNTSGSDDHTKIKPDTSSTGAAPPTPTPKFPQFSSFRSPDHRKPTLPLPIQTKSETSSPASATVPQFPNSATNPNASIYFPSMHAQSPGGQYGGGMLPTPVFNQINQQLQQQQQQQQQQQAHQQFQQYHQGPPPPQQQQQQQQSQQNQQQNQQQQQLPQQQQQDQQNQHPGGQVAQPESAIRFRPPTGVTNQYNNGEQTPLSGLPSRYVNDMFPFPSPSNFLGPQDWPSGTTPTTHMPPYFINMMPLTSTTAGPPTTFPSTGNTKHGLGIKTDGNMSPTAYHVQASQGNRGDDGGTNPTTAGSTVDNKLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATN8</accession>
    <name>G3ATN8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HTH TFE/IIEalpha-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_62898</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIE alpha subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377034.1">
        <property type="nucleotide sequence ID" value="XM_007376972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATN8"/>
    <dbReference type="FunCoup" id="G3ATN8">
        <property type="interactions" value="139"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATN8"/>
    <dbReference type="GeneID" id="18874517"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62898"/>
    <dbReference type="eggNOG" id="KOG2593">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035744_2_0_1"/>
    <dbReference type="InParanoid" id="G3ATN8"/>
    <dbReference type="OMA" id="DAIKWKV"/>
    <dbReference type="OrthoDB" id="361102at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005673">
        <property type="term" value="C:transcription factor TFIIE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001097">
        <property type="term" value="F:TFIIH-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001113">
        <property type="term" value="P:transcription open complex formation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039997">
        <property type="entry name" value="TFE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017919">
        <property type="entry name" value="TFIIE/TFIIEa_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002853">
        <property type="entry name" value="TFIIE_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024550">
        <property type="entry name" value="TFIIEa/SarR/Rpc3_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097:SF7">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02002">
        <property type="entry name" value="TFIIE_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00531">
        <property type="entry name" value="TFIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51344">
        <property type="entry name" value="HTH_TFE_IIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH TFE/IIEalpha-type" evidence="5">
        <location>
            <begin position="5"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="230"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="237"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="269"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="323"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="357"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW31001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="390" mass="44101" checksum="E5E937F5953D5213" modified="2011-11-16" version="1">MDDSVRSLIRFVSRGFYSTPYVLILDAVLRHSVLSEDDLIHLLGIKRKELRSLCNRLVEDRLLINHIQREENSQQKFVTRTYFFIHITEAIDSIKWKVHSIVNHLKQEMSTFGNPQGYVCPRCGKKVSQIDAISLLSEDKTEFVCDVCQGILIEDDSSQQATLKQEKLEKLMKQVDPIIKYLKIIDDSNIQDNDFESALIQSIPAQSSSTASYTLSNKISSRSRSVNSSAAAASKSQATLHVSITANDENYEREQEEKERRRQKLEQNALPSWHSSSTVGQSSATFGKFDDDEREGTPSSIKEEDEEKPSIDDIEMKEEVESTAEQSSTMATPEPSTNQFTNTSTNASELKDKEAQDALAAYYAQLAEQEAAEDEDDDDDEDDFDDLLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATP9</accession>
    <name>G3ATP9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleolar protein 58</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_63123</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for pre-18S rRNA processing. May bind microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377246.1">
        <property type="nucleotide sequence ID" value="XM_007377184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATP9"/>
    <dbReference type="FunCoup" id="G3ATP9">
        <property type="interactions" value="1669"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATP9"/>
    <dbReference type="GeneID" id="18874604"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63123"/>
    <dbReference type="eggNOG" id="KOG2572">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015495_5_2_1"/>
    <dbReference type="InParanoid" id="G3ATP9"/>
    <dbReference type="OMA" id="MGMRSNW"/>
    <dbReference type="OrthoDB" id="6780543at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017069">
        <property type="term" value="F:snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902570">
        <property type="term" value="P:protein localization to nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000452">
        <property type="term" value="P:snoRNA guided rRNA 2'-O-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.90:FF:000003">
        <property type="entry name" value="Nucleolar protein 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4070:FF:000001">
        <property type="entry name" value="Probable Nucleolar protein 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012974">
        <property type="entry name" value="NOP58/56_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF1">
        <property type="entry name" value="NUCLEOLAR PROTEIN 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08156">
        <property type="entry name" value="NOP5NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Nop" evidence="10">
        <location>
            <begin position="283"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="430"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="435"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="455"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="484"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW30275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="512" mass="56967" checksum="C76C3A2160EBC4CF" modified="2011-11-16" version="1">MAYVLTETAAGYALLKASDKKIHKSSTLIEDLDSASKVASQFKIHRFEKFQSAANALEEANAIIEGKVSDNLRKLLEDAKSDKKATLIVSEAKLGNAINKLGLNFSVVSDAASLDLHRAIKQFLPELLPGLDGAVLNQMSLGLAHSLGRHKLKFSPDKVDTMIIQAIALLDDLDKELNTYAMRCKEWYGWHFPELAKMITDSVAYARIILTMGVRSNASETDLSEILPEEVEEQVKTAAEVSMGTEITEDDLNNIKALAEQIVDFAAYREQLSNYLSSRMKAIAPNLTAMVGELVGARLIAHAGSLTSLAKAPASTIQILGAEKALFRALKTKHDTPKYGILYNASLVGQASGKNKGRIARVLAAKAAISLRIDCFDEERDDSDDFGLENRAKVESRLSQLEGRDMRTTSRVVKEQSKIEITEARAYNADADAAAVEEDSDDETSETEEIVEKKKEKKEKKEKKDKKDKKRKRDDDEEEESSKKSKKEKKEKKEKKDKKEKKDKKDKKKEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATU0</accession>
    <name>G3ATU0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_68431</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377287.1">
        <property type="nucleotide sequence ID" value="XM_007377225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATU0"/>
    <dbReference type="GeneID" id="18875217"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68431"/>
    <dbReference type="HOGENOM" id="CLU_977172_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATU0"/>
    <dbReference type="OMA" id="HCHCTAT"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="285" mass="28015" checksum="6C56423D4B4F63CB" modified="2011-11-16" version="1">MAKHDVKRVPEGNLQNLADISVSAWTSTSTTSVTTVCTATSANTATSTATVAVSADGTATVTVTTNDGASATASARANASATAAATAHATDINSASAAVAATAAASASASAAASASASAAASAAATNYATAAATTAATAIASTPIDPTATSAAAGEAAGAAIDASIDASIDAAINAVDPTTIVPSISVTCAPTVSPTVSSAFSVTTTVTTSANVAPGSSPYERQGNLNPKCDESHDLGNTETFTTAEEFMQKLYTELDHIVCLRNVLTIERINTFLDWLDKKTGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU07</accession>
    <name>G3AU07_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Eukaryotic translation initiation factor 5A</fullName>
            <shortName evidence="8">eIF-5A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_63230</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Translation factor that promotes translation elongation and termination, particularly upon ribosome stalling at specific amino acid sequence contexts. Binds between the exit (E) and peptidyl (P) site of the ribosome and promotes rescue of stalled ribosome: specifically required for efficient translation of polyproline-containing peptides as well as other motifs that stall the ribosome. Acts as ribosome quality control (RQC) cofactor by joining the RQC complex to facilitate peptidyl transfer during CAT tailing step.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="8">eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the eIF-5A family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377354.1">
        <property type="nucleotide sequence ID" value="XM_007377292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU07"/>
    <dbReference type="FunCoup" id="G3AU07">
        <property type="interactions" value="1036"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AU07"/>
    <dbReference type="GeneID" id="18874652"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63230"/>
    <dbReference type="eggNOG" id="KOG3271">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102600_1_0_1"/>
    <dbReference type="InParanoid" id="G3AU07"/>
    <dbReference type="OMA" id="KDDVRMP"/>
    <dbReference type="OrthoDB" id="9975114at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045901">
        <property type="term" value="P:positive regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006452">
        <property type="term" value="P:translational frameshifting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04468">
        <property type="entry name" value="S1_eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000007">
        <property type="entry name" value="Eukaryotic translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000034">
        <property type="entry name" value="Eukaryotic translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001884">
        <property type="entry name" value="IF5A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048670">
        <property type="entry name" value="IF5A-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020189">
        <property type="entry name" value="IF5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019769">
        <property type="entry name" value="Trans_elong_IF5A_hypusine_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00037">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11673">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 5A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01287">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21485">
        <property type="entry name" value="IF5A-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003025">
        <property type="entry name" value="eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01376">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00302">
        <property type="entry name" value="IF5A_HYPUSINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="7 8" id="KW-0385">Hypusine</keyword>
    <keyword evidence="10" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Translation initiation factor 5A C-terminal" evidence="9">
        <location>
            <begin position="84"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW30383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="157" mass="17118" checksum="0242F99AB7C4CFC3" modified="2011-11-16" version="1">MAEEDHTFETADAGAALTYPMQCSALRKNGHVVIKGRPCKIVDMSTSKTGKHGHAKVHLVAIDIFTGKKLEDLSPSTHNMEVPNVSRQEYQLLDIDDGFLSLMTQDGDTKDDVKVPEGELGEKIQSEFDEGKDLLVTIISAMGEEAAISYKEAPKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU22</accession>
    <name>G3AU22_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATP-dependent RNA helicase DBP9</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">ATP-dependent RNA helicase dbp9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">SPAPADRAFT_157367</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits and is required for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30398.1"/>
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    <sequence length="578" mass="66503" checksum="20434CFBA9FD8A69" modified="2011-11-16" version="1">MSDSAASQAYLDEEASWDSFNLDPRLLQALDQLGFEKPTLIQSSSIPLALEEKRDIIAKASTGSGKTGAYCIPIVNNLLTRSTDEHEIKSIILVPTRELSNQVYEFLEKLLVFNNQKINILNLSSNYSDQVLNSLLINKPEIIISTPAKLIQTLEKNSKGLIDLSTVSNLTIDEVDLVLSFGYLDDLKKLESYLPIKKNLQTFLMSATINEELNDLKMRYCTKPAILKLNDETAEKQSLVQYYAKTTEFDKFLLCYVIFKLNLIKGKTIIFVNNIDRGYRLKLYLEQFGIRCCILNSELPINSRLHIVEEFNKNVYHLLIATDETNDFTVEKEEDSEGEREREEQEQAQEEKKESKKSKKKGAKKDSEYGVSRGVDFRNVACVLNFDLPTTSKAYIHRIGRTARAGKSGMALSFIIPLKEFGKHKIANLATAKKDEKVLRRIIKQQSRNGFEIKPYQFDMKQVEGFRYRAEDAFRAVTQTSVREARVKELKNELINSEKLKRFFEENPQDLATLRHDKELHPTRIQSQLKRVPQYLLPESARSDPKNIGFVPFHKNKVHKNRKRKSTKKSDPLKTFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
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        </recommendedName>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="entry name" value="F-box-like_dom_sf"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="7"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
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            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="412" mass="47061" checksum="F4667F3D3A98EAD9" modified="2011-11-16" version="1">MTSYCGCFPIIELPNDIIGEICAILNQVDVLSLMLVNKTLYQLGLRKLYHSIYIGSTFPRLLVSGHIYTAFNTKYTIVTSAKVFVSALRHTSNAQLVKEIVTTDFEPDDYMSIVSLFPWVNIVLEYEKSWARFTCLDLRLLRGLELTIDNYRFFQDKVADTNYSIKELTMQSGDGLFNIIPKLKGLESLHISPVTTETLMGLDLAGVSKLKIKELFLQDCGPDCEGGRLFHQVEELFNLDCIKSFGFSMLYNMRYRDLESIIPRLTNVRNIFIQGGINDYGQILMELKPNSLHYLCLHGNSVYTSKESNIDFQQLTKIIENQEQSLSTLCVSRSVPHFMKNTCVNRFHNYGCCKTSDESQLRLLEQILGKGRRFPSLDTVILNGRYHLIKRHMQKGIEIIPLLIGSELDNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUF5</accession>
    <name>G3AUF5_SPAPN</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_52608</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007377440.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUF5"/>
    <dbReference type="GeneID" id="18871700"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52608"/>
    <dbReference type="HOGENOM" id="CLU_505427_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUF5"/>
    <dbReference type="OrthoDB" id="10492065at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="539" mass="62034" checksum="47B9C64AF0326BF5" modified="2011-11-16" version="1">MASDNEDPIDTADLENQWYNRQITKINLAEIYIHNYDHNLNHIYNETCHMKEIEELNTVEFWKDFIVGVEEFYTNIRTYFNNPAGFKITQGYIHNLEYGNGPYAMDDLKIQLVLLDETVVGFIKSRSQLIDEICAFTGNKTATMKDVYEYFNSKRTLTSAETCYEPEELVMKDALRIVFVTAMGFRRQTLLWMLALSDEIKKKKSECFQFLNSIPFEFILAYISMKQDNDASGGVLRGYIPFTNFLANEYILYKEDKEAYKMKPFETVQYLLKTDPEVDSQHFTNLGNVYTGKRATLSQALASKTHDKRPERFTESPPSFAKRKLHSSFERASKTRRIERPSFRDRSNIPYSRVHTPSAGCDRYMPRISETNLIPVNENRFKSRPVGPRDDTPIGSLSRPTSPRENSPLAPPTPVSSIANLDNIRWPERDPDTKKPETVVTSSIYPKSNDENETPHLPIAPMDRRPSVDVKPLSYKEIIHKYTRTSDGATIGSNLLGSSCGKKNKEVIKSDREQPEEDIKVIGILEEHKSTRSESTQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUF8</accession>
    <name>G3AUF8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_68621</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377505.1">
        <property type="nucleotide sequence ID" value="XM_007377443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUF8"/>
    <dbReference type="STRING" id="619300.G3AUF8"/>
    <dbReference type="GeneID" id="18875227"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_68621"/>
    <dbReference type="eggNOG" id="ENOG502RQFA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1074058_0_0_1"/>
    <dbReference type="InParanoid" id="G3AUF8"/>
    <dbReference type="OrthoDB" id="4026506at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="220" mass="24615" checksum="59B691C0E2A803E2" modified="2011-11-16" version="1">MALEDNSDNDTVDDEVKELMSAALTYGNDQYFSPDTQPTSMEGSSSDFSTLRGNGGKKLQKLTDEHGNEYLLQPNGYVHPIRKPEELFIHPDRPQDHQIMYGTNNMYTPDVERRPTSSSKSSRKLKSFIDLSRNGKHSTPDQLREFQIKVLDSIQYSGDLVLGTTNRYGDVDSTAKIIRSATNSTRSSSISSSFRTGTLRTSSISSPSQNSVKFEKKKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUL1</accession>
    <name>G3AUL1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWR1-complex protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_63403</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Involved in chromosome stability.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SWC5 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377538.1">
        <property type="nucleotide sequence ID" value="XM_007377476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUL1"/>
    <dbReference type="FunCoup" id="G3AUL1">
        <property type="interactions" value="471"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUL1"/>
    <dbReference type="GeneID" id="18874734"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63403"/>
    <dbReference type="eggNOG" id="KOG4776">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062474_1_0_1"/>
    <dbReference type="InParanoid" id="G3AUL1"/>
    <dbReference type="OMA" id="LDWAAYV"/>
    <dbReference type="OrthoDB" id="445677at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011421">
        <property type="entry name" value="BCNT-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027124">
        <property type="entry name" value="Swc5/CFDP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48407">
        <property type="entry name" value="CRANIOFACIAL DEVELOPMENT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48407:SF1">
        <property type="entry name" value="CRANIOFACIAL DEVELOPMENT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07572">
        <property type="entry name" value="BCNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51279">
        <property type="entry name" value="BCNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BCNT-C" evidence="5">
        <location>
            <begin position="182"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="77"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="257" mass="29275" checksum="77CE25930D51417A" modified="2011-11-16" version="1" fragment="single">MAKIKEQPVEEPEDDYDEELDEDYDPTKAASDDEEDSEDDEVVPGRPASSLHIGRFITDESGLVKNSTSLDIDSIFDDMKRGEAKETGHSETEEREREREQEQELHKNKTDAKQIKIQTSYTYAGKLITETKLVDADSEEAKAYLNSTSSVLSSDDGKNRSEVPVVRKDVMTGEMVQLRIKLKRPSLIDRFNGRNKKSLSTLEKSRLDWASFVDKRKIGDELKRYNKAGYLDKQAFLGRVDVKRDEKYVEAKEAAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUR7</accession>
    <name>G3AUR7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_63451</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377593.1">
        <property type="nucleotide sequence ID" value="XM_007377531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUR7"/>
    <dbReference type="FunCoup" id="G3AUR7">
        <property type="interactions" value="248"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUR7"/>
    <dbReference type="GeneID" id="18874758"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63451"/>
    <dbReference type="eggNOG" id="KOG2190">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022670_4_1_1"/>
    <dbReference type="InParanoid" id="G3AUR7"/>
    <dbReference type="OMA" id="SIAKEPH"/>
    <dbReference type="OrthoDB" id="442947at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="79"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="173"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="291"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="374" mass="40527" checksum="36E5AE250131081D" modified="2011-11-16" version="1">MTESLFDTNFTSENEPKPVDEQVQPTTDEQPTSVSTESQPSNSESSEQAEEVPVSASSSTNHDQSTTLPSLNDKSNGDAVINVRVLVSAKEAGCLIGQNGKVIDGIRDETNTKAGISRLQPGSHERILTVSGKLDNCAKALSYFAQALCDSTIETYNYFPLKQLSANPCIENETTILRLLVPNAQMGTLIGSKGLRIQQIQSNYHISMIASKSFLPGSNERLVELQGTVNDLYDALRIIARCLIEDISALGNTNYYVPRGHHSQYNGVNTTNGVSSTTPTNGRNFARKSSHIATTSLSFPNDIVGALIGKNGSRIQGVRKVSGATIGISEEVEGKNERIFTISGSPHAVEKAKGLLYHNLQREEQRRAEAEKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUZ7</accession>
    <name>G3AUZ7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Large ribosomal subunit protein bL21m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_52714</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL21 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377621.1">
        <property type="nucleotide sequence ID" value="XM_007377559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUZ7"/>
    <dbReference type="FunCoup" id="G3AUZ7">
        <property type="interactions" value="213"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUZ7"/>
    <dbReference type="GeneID" id="18871713"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52714"/>
    <dbReference type="eggNOG" id="ENOG502S1KI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061463_2_1_1"/>
    <dbReference type="InParanoid" id="G3AUZ7"/>
    <dbReference type="OMA" id="ITKEPRY"/>
    <dbReference type="OrthoDB" id="266718at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028909">
        <property type="entry name" value="bL21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036164">
        <property type="entry name" value="bL21-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21349">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21349:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL21M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00829">
        <property type="entry name" value="Ribosomal_L21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141091">
        <property type="entry name" value="L21p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00044129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW29855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="162" mass="18458" checksum="5F4C7F69DB4757B9" modified="2011-11-16" version="1">MFASIRSLRPCVVPRMLPRMLPRMYSTATQSAAQATTAVSPLSSLKINSNGNQHLYAIFKLHNMPYLVTKGDLIYLNYKLKNAEIGDELVLNDVTTLGSPSYTYTEPNGIDSSLYSLKANVVEITREPKYQVTRTKQRQRRVKTFQVENFQTVLRINELKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVC0</accession>
    <name>G3AVC0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">60S acidic ribosomal protein P0</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_63750</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. uL10 forms part of the P stalk that participates in recruiting G proteins to the ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377905.1">
        <property type="nucleotide sequence ID" value="XM_007377843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVC0"/>
    <dbReference type="FunCoup" id="G3AVC0">
        <property type="interactions" value="1484"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVC0"/>
    <dbReference type="GeneID" id="18874895"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63750"/>
    <dbReference type="eggNOG" id="KOG0815">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053173_1_1_1"/>
    <dbReference type="InParanoid" id="G3AVC0"/>
    <dbReference type="OMA" id="DMNPFKL"/>
    <dbReference type="OrthoDB" id="10259902at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05795">
        <property type="entry name" value="Ribosomal_P0_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1730:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050323">
        <property type="entry name" value="Ribosomal_protein_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030670">
        <property type="entry name" value="uL10_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039087">
        <property type="entry name" value="L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="7">
        <location>
            <begin position="109"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="284"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="294"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR039087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW30139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="312" mass="33288" checksum="573C91C779961008" modified="2011-11-16" version="1">MGGARDKKVQYFSKLRELLEEYKSIFVVGVDNVSSQQMHEIRKALRGDAVVLMGKNTMVRRAIRGFLSELPEFEKLLPFVKGNVGFIFTNADLKTIRDTVVSNVVAAPARAGAVAPLDVWIPAGNTGMEPGKTSFFQALGVPTKIARGTIEIVSDVKVVEADQKVGPSEATLLNMLNISPFTYGMTVVQVYDNGQVFPSSILDITDDELISHFVSAINAIASISLAVGYPTLPSVGHSLINNYKNVLALSIATDYTYEGSEAVKDRLANPEAYAAAPAAAASAGAEESGAAEEAAAEEAEESDDDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVG0</accession>
    <name>G3AVG0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63791</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377944.1">
        <property type="nucleotide sequence ID" value="XM_007377882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVG0"/>
    <dbReference type="STRING" id="619300.G3AVG0"/>
    <dbReference type="GeneID" id="18874915"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63791"/>
    <dbReference type="eggNOG" id="KOG0590">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1242744_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVG0"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="223" mass="26016" checksum="F3FF4774BED309BF" modified="2011-11-16" version="1" fragment="single">MKDAIFKQILTGVRYLHRKNIIHGDLKPENFLLDSDGIIKISDFGYSLDLNTNDYQQEILLNPRTIYCGTNSFKAPELFQIEHDINHNEFNFSDFFTSAEMLFKSWDYWALGMIYFQLFLMKAPWSMANPLQPMNIAYIKYSKEYPDSADKVHALMNELNSKHSTFKSNPAVALFKSLHYDSRESILGLLNPNPEKRLTPDDLLNTNWLTQVYADPKEFIKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVQ9</accession>
    <name>G3AVQ9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_144523</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377990.1">
        <property type="nucleotide sequence ID" value="XM_007377928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVQ9"/>
    <dbReference type="FunCoup" id="G3AVQ9">
        <property type="interactions" value="375"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVQ9"/>
    <dbReference type="GeneID" id="18870634"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_144523"/>
    <dbReference type="eggNOG" id="KOG4209">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_23_3_1"/>
    <dbReference type="InParanoid" id="G3AVQ9"/>
    <dbReference type="OMA" id="FRMPTRY"/>
    <dbReference type="OrthoDB" id="4726at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12306">
        <property type="entry name" value="RRM_II_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF12">
        <property type="entry name" value="EUKARYOTIC INITIATION FACTOR 4B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="94"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="188"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW30224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="217" mass="24086" checksum="357F78EA22452253" modified="2011-11-16" version="1">MSDEVPISQQQHYPDHQQEQPAYEHGNSEDQTSPDANVSLIIHPEQDESNRERIEEMEREAAKLRELHSQLSNDTAMSGITPQTDEEKRDVDARSIYIGNVDYGSTPLELQQHFSSCGIVNRVTILMNKHTGQPKGFAYLEFADVDGVNKAVATLDGSMFRDRQLKVSAKRTNIPGIGIKRGAFRGGRGGRGSFRGAPRGRGRGRGAFRGGARFTPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVW3</accession>
    <name>G3AVW3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_52853</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377774.1">
        <property type="nucleotide sequence ID" value="XM_007377712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVW3"/>
    <dbReference type="GeneID" id="18871726"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52853"/>
    <dbReference type="HOGENOM" id="CLU_860974_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVW3"/>
    <dbReference type="OrthoDB" id="4025890at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW30008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="323" mass="37363" checksum="0E94F49251513D9E" modified="2011-11-16" version="1">MVKARHDTISQLKLKLSRKVNQKFPHKSRLSFKTKHEYNQFIAKMIKVVLSHFKFVTDLPNKTYAEDLDLLKVYSHHAKGHIFLVQSISLMLIHETLADVTIDVQGKIQPQLVLCKLGILAGDYCILVQHNHIEICSIPQVKQYMRRTQPKSEAYFNPVDLHALFRLFIDVESYEQEVKRDNWSVSHSYRAMSLTGFTDRSLIDLLSSNVFSGEFVLPEKFLGHLNNFIHGSYPQFPHVQDVGELVMNKRTNRVGVVVHKSDELHTIYTTFHQLDKCLSQRLVKVSLNTPDLLMILDQMGLDVIGLLYFKGFTFDNGMQFIPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADW9</accession>
    <name>G3ADW9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">SPAPADRAFT_57749</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372105.1">
        <property type="nucleotide sequence ID" value="XM_007372043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ADW9"/>
    <dbReference type="FunCoup" id="G3ADW9">
        <property type="interactions" value="121"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ADW9"/>
    <dbReference type="GeneID" id="18872181"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57749"/>
    <dbReference type="eggNOG" id="KOG0393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_21_1_1"/>
    <dbReference type="InParanoid" id="G3ADW9"/>
    <dbReference type="OMA" id="KDTWFPE"/>
    <dbReference type="OrthoDB" id="4031310at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030011">
        <property type="term" value="P:maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090338">
        <property type="term" value="P:positive regulation of formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04132">
        <property type="entry name" value="Rho4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000983">
        <property type="entry name" value="Rho family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="254"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="270"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="299" mass="33858" checksum="524BC7994940D50F" modified="2011-11-16" version="1" fragment="single">MTTATQLKRRSFIGRSPKQPVEEPTFNPYEVANQQTHRRNPSFQQYNEPKIIIRDDPVIPDYKVQDVFEKRSNYNMKLVVVGDGGCGKTCLLVSYAQQKFPEIYVPTVFENYVTTVQSPNGKTIELALWDTAGQEEYDRLRPLSYPDVDVLLICFSLDNIVSLHNVKEIWFPEVNHFCPGIPIILVGTKSDLHSDIDPDVPIQLATEINAVGYVQCSAKTMFNIKTVFNFALSHFQRKMELQEQYERTSKNRLSKVLGNGGGSNGGHYASHSRQHSSTSRRGHLNSTSFDSTALLDQPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADX1</accession>
    <name>G3ADX1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription activator GCR1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_57752</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372107.1">
        <property type="nucleotide sequence ID" value="XM_007372045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ADX1"/>
    <dbReference type="STRING" id="619300.G3ADX1"/>
    <dbReference type="GeneID" id="18872182"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57752"/>
    <dbReference type="eggNOG" id="ENOG502QQ7G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1171242_0_0_1"/>
    <dbReference type="InParanoid" id="G3ADX1"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060963">
        <property type="term" value="P:positive regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052146">
        <property type="entry name" value="HOT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022210">
        <property type="entry name" value="TF_GCR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784:SF2">
        <property type="entry name" value="HIGH-OSMOLARITY-INDUCED TRANSCRIPTION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784">
        <property type="entry name" value="PROTEIN MSN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12550">
        <property type="entry name" value="GCR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription activator GCR1-like" evidence="2">
        <location>
            <begin position="134"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW34695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="237" mass="26006" checksum="3579C25676EFFF71" modified="2011-11-16" version="1">MLPLPNLLGEGNAPSNKPGSVAVQYMSTIPPSELRHTNQSRTSFFGDDDIDHNIDELSGDNGGNTNAAGTGSGGTTAGTAGSDSTTGTAAVTTVSAPGEPNDDTSSSNGDDKPSKKRKSDESSKANLSLDIPKYRLERSLKSISDIWKEYEYGLNDKPPLKLLEIKYGTKWRNDTESRTFLRRKKIYDAIENGKAKGYTEDQVIRELEEYRSYDRNGTIKKKPLSWLNANMPDKYDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE74</accession>
    <name>G3AE74_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_48588</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373020.1">
        <property type="nucleotide sequence ID" value="XM_007372958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE74"/>
    <dbReference type="GeneID" id="18871241"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48588"/>
    <dbReference type="HOGENOM" id="CLU_567622_0_0_1"/>
    <dbReference type="InParanoid" id="G3AE74"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="63"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="414"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="193"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="430"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW35608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="481" mass="54774" checksum="8773E6057F4E4CFD" modified="2011-11-16" version="1">MTSMFSIIKRIWNIFTPAEEAVPVVELNSNVDSSPTKTPSCRSEFRDLLPEFRKYYPKENCSDLSSVPSNFNPNPQESSTLKSSASDHKHKHGFPKLSNCFKIWGNDKFSTATKIDRQFPVTSSLPPNVVDQKFTEHSSQFIQSQFGLSTQMSRELKKLKVRSSWNELKDIQRELLDRYASECFQVCRCETTIRQLREKLEELEVELVNAQKEYEECGESISSISTELGSSINSLENSIRDICGKIDNTTGFVTSLGEQSPITSPKINPFSTKHRKGSILSTLSQMFHQEKSNSKANFEIHSVSPEIFEPSIRYNSLHAIGKLSCSQVVNVSGIVYSDIFTSGEGLCPEGIVHEAPGNNSDVSFSNQSESTYLSRSSWQNERFKWFDFKGRIASKFMEKTNNSTLERKQRQFEELKEHKLRKQQRKERQSTGYNSLDINIGQNDSEDDSFGDSKLIRFRAVARSNPGSGIIRKLKQLKKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEB7</accession>
    <name>G3AEB7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SVP1-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_131732</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Vacuole membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373118.1">
        <property type="nucleotide sequence ID" value="XM_007373056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEB7"/>
    <dbReference type="FunCoup" id="G3AEB7">
        <property type="interactions" value="388"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEB7"/>
    <dbReference type="GeneID" id="18869604"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131732"/>
    <dbReference type="eggNOG" id="KOG2111">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025895_0_1_1"/>
    <dbReference type="InParanoid" id="G3AEB7"/>
    <dbReference type="OMA" id="GGPQCMC"/>
    <dbReference type="OrthoDB" id="1667587at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010314">
        <property type="term" value="F:phosphatidylinositol-5-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW35706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="400" mass="43836" checksum="087F1C21C6447655" modified="2011-11-16" version="1">MATTTTNTSSSSSGPGTATPGLSKDSRILCINFNQDQGCFAIGHESGFLVYNTNPIDLRVKRNFPASETTTTTTTTTTNGGGTGIGQISMLHRTNYLGLIGGGKSPKFAPNKLVIWDDLKRKVSLTLEFDSPVINVLLSRIRIIVVLRDKIIVYGFSAPPKMLTSYDTIDNEFGIADLASVSNSAATSASSISSLSSTSTKSQTLAFPARSMGQIQIVDVGQNIIHIIKAHKSKLRCITLNKTGTLVASASVTGTIIRIHSTHDQVLHYEFRRGLDRAIITSMKFSPDDSRLAVLSDKHTLHIFNIGEAPNRQHVLNRLSGSVPIPQYFKSTWSFCCVNTNKYHLDSQPDQGVIGWSGTDCVIIIWQNKRIWEKYVIVETSNEATTYEIVRSSWKSLESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>G3AED8</accession>
    <name>G3AED8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase</fullName>
            <ecNumber evidence="4 12">5.3.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">5-proFAR isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 12">Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_130719</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:15469"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="5.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.16" evidence="4 12"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373138.1">
        <property type="nucleotide sequence ID" value="XM_007373076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AED8"/>
    <dbReference type="FunCoup" id="G3AED8">
        <property type="interactions" value="223"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AED8"/>
    <dbReference type="GeneID" id="18869522"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_130719"/>
    <dbReference type="eggNOG" id="KOG3055">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065050_0_0_1"/>
    <dbReference type="InParanoid" id="G3AED8"/>
    <dbReference type="OMA" id="IEWNKTH"/>
    <dbReference type="OrthoDB" id="446074at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00009"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04723">
        <property type="entry name" value="HisA_HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000110">
        <property type="entry name" value="1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011858">
        <property type="entry name" value="His6/HISN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02129">
        <property type="entry name" value="hisA_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090:SF2">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8 12 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW35726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="275" mass="29779" checksum="7172CF476417D341" modified="2011-11-16" version="1">MTKFRGCIDIHSGQVKQIVGGTLTQDDTTHTNSTTTNFVSSFPSSHYAGLYHDHNVTGCHVIKLGSNPANDEAAELALKTWPGGLQVGGGINYDNASEWLDKGASHVIVTSYLFSKVGEDMAFDWDKLKKVSEKVGKENLIVDLSCRTVVNQGESQWVVAMNKWQTLTTSVLSETFLSEVGEYCDEFLIHAADVEGLCQGIDETLVEKLGQWCPSGFEGKIVYAGGAKSAQDLDVVKQLSGGKVDLTYGSALDIFGGKLVKFDDVVAWNKNQSAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEE4</accession>
    <name>G3AEE4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58929</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373145.1">
        <property type="nucleotide sequence ID" value="XM_007373083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEE4"/>
    <dbReference type="STRING" id="619300.G3AEE4"/>
    <dbReference type="GeneID" id="18872686"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58929"/>
    <dbReference type="eggNOG" id="ENOG502QVPF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_647524_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEE4"/>
    <dbReference type="OrthoDB" id="4494341at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="424" mass="50160" checksum="0BAA5CFE6344C625" modified="2011-11-16" version="1">MPEEEEPIFDHGVVRERKLNVSVLSLNIKFNYDRILSTLVYLLHNGLSSNITQAQITEISDMIIQYYMYIHNSMNKMNEFYKANPPTTVQEMNLINFIKNHSFSFVDRAMMLLHKKILFGDDTSNEVSAKQSKQGFELNVDKNRGGVLSLSQYEDTFGHIQEANIIKNFNNSSISLLKFNPSEDFSYGNMHNNLIPSILHNLNDFLKYNDFIKFGKYNWYIKRTIPLDSIILMFIIITVKFKYEFFTINELCIYVKLINKALFILNRKWFKNEKYKRMLSLTNLTWEYILKRYNIVQLINQFNDEMPNNRIEFFDYQVKAMVNMNELFDVMDVPQPIDFPYKLDAHGKMPSKRSHSLGSRVNNVSVNSLVLNPESKLELAQLNEKIYYDLRNNFVDVNDYCAFYTSLENILHELMDFMNQQRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEF0</accession>
    <name>G3AEF0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prefoldin subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_53903</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373150.1">
        <property type="nucleotide sequence ID" value="XM_007373088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEF0"/>
    <dbReference type="FunCoup" id="G3AEF0">
        <property type="interactions" value="743"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEF0"/>
    <dbReference type="GeneID" id="18871819"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_53903"/>
    <dbReference type="eggNOG" id="KOG1760">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130032_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEF0"/>
    <dbReference type="OMA" id="KFGRAIN"/>
    <dbReference type="OrthoDB" id="10250441at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071629">
        <property type="term" value="P:cytoplasm protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23165">
        <property type="entry name" value="Prefoldin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000005">
        <property type="entry name" value="Prefoldin subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016661">
        <property type="entry name" value="PFDN4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21100">
        <property type="entry name" value="PREFOLDIN SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21100:SF9">
        <property type="entry name" value="PREFOLDIN SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016477">
        <property type="entry name" value="Prefoldin_subunit_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="76"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR016477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="130" mass="15249" checksum="C917E094D9C27A92" modified="2011-11-16" version="1">MELLPEGQRNTATEVTWEDQQRINKFSTIIAKKDEQSAILEKLKTDKEYLDDLSLEIELLDEDEKIQYKIGDAFMLLKVEKAVEKIEQDNEKLSTRINEVCDLIDSYDDQLGELKKYLYAKFGNNINLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEH0</accession>
    <name>G3AEH0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Histone-lysine N-methyltransferase, H3 lysine-4 specific</fullName>
            <ecNumber evidence="3 14">2.1.1.354</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_133304</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalytic component of the COMPASS (Set1C) complex that specifically mono-, di- and trimethylates histone H3 to form H3K4me1/2/3. COMPASS recognizes ubiquitinated H2B on one face of the nucleosome which stimulates the methylation of H3 on the opposing face.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60260"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.354"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60272"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15540"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-methyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60268"/>
            <dbReference type="Rhea" id="RHEA-COMP:15540"/>
            <dbReference type="Rhea" id="RHEA-COMP:15543"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the COMPASS (Set1C) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.354" evidence="3 14"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372144.1">
        <property type="nucleotide sequence ID" value="XM_007372082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEH0"/>
    <dbReference type="FunCoup" id="G3AEH0">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEH0"/>
    <dbReference type="GeneID" id="18869737"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133304"/>
    <dbReference type="eggNOG" id="KOG1080">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004391_1_0_1"/>
    <dbReference type="InParanoid" id="G3AEH0"/>
    <dbReference type="OMA" id="ERLPCLC"/>
    <dbReference type="OrthoDB" id="308383at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140999">
        <property type="term" value="F:histone H3K4 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030437">
        <property type="term" value="P:ascospore formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905088">
        <property type="term" value="P:positive regulation of synaptonemal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902275">
        <property type="term" value="P:regulation of chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903341">
        <property type="term" value="P:regulation of meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055092">
        <property type="term" value="P:sterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007130">
        <property type="term" value="P:synaptonemal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024657">
        <property type="entry name" value="COMPASS_Set1_N-SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044570">
        <property type="entry name" value="Set1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017111">
        <property type="entry name" value="Set1_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048669">
        <property type="entry name" value="SET1_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024636">
        <property type="entry name" value="SET_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45814">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45814:SF2">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11764">
        <property type="entry name" value="N-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21569">
        <property type="entry name" value="SET1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11767">
        <property type="entry name" value="SET_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037104">
        <property type="entry name" value="Histone_H3-K4_mtfrase_Set1_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01291">
        <property type="entry name" value="N-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00508">
        <property type="entry name" value="PostSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51572">
        <property type="entry name" value="SAM_MT43_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 14" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5 14" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 14" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="16">
        <location>
            <begin position="918"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="17">
        <location>
            <begin position="1044"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="356"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="614"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="864"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="74"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="93"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="634"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="672"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="692"/>
            <end position="711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR037104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW34732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1060" mass="121901" checksum="ECCCD3A6664A9446" modified="2011-11-16" version="1">MSYNRGYSHRGYSGSSGTGSYRDNRYRSGRTDSSFRRQEDHPGATLTLNKSSHYDDSNNNNNRPHHLGSRFSAGSRNGGSSTPNGSNSDRNGKSSGTPTKGSQTPVRRIEGSDLITGENPKDSDFNKLLHHKDYTDTIPFSRPVDSKKNYKVIYDPEFDKSLSKDEKKTKTKKLRFHGEGMDDKELVDPRLSHLQSYFQKPNKKSKKFPFKQLPQPRFVFDKDSLGSAPRTQLVVWDLPATTSEIYLSNYFRSYGDPIEDMKFINDPENAVPLGIATFKFQGNPEKATRLAQKLVQSIKSEMPKIDGVLLKIGLNDNEDELLNKKIELARDKLKAIKLKREEEERRRQKVIEEQKRREEELKKKELEEQKKKKEQEAAAAAQEKQKVQESDMKYKPNTTILSIRRQNKVVSGVFLPKDLNKYVKDRAFILIHDKYVPTKKISSQDIKRVLNKYDWTRVLSDRTGFYVVFNSLNECERCFMNEDGRKFFEYRLYMEMAIPEGFSSSPSEEEEIPTSKKNDVIEEATNILIKELETFLAKDIRERIIAPHILDLLGHEHYPKLVEELKAQEAASKPKVINTNDQLKQNALSILEKKRQLQQQQLLPSFRKLQQQNELKRRNARKTLVPMQHALNFDSDDEDEDEEETSRSATPAIQSLKRERSSTVTSADEFIEPEHKKPKKDNLKRSMLYESSSDEEMDEEMKEEEDKEVVDEEGKAEVKIEEEAKPEDTEIDYSKIAAHFQPTEDSPTTVYPESPLSSIFDLEALQSTLKDVEDLELAQQVLAEEGISPSHLKNIAYWAWKQKDIKLSSDEIAEEVENIEVLPTHLESESGAFRSDGYKKINDADKIHYLPHRRKVHKPLKTIQYEDDDEERPNDAASAVQSSRVNRANNRRFAADITAQIGNESDILSLNALTKRKKPVTFARSAIHNWGLYAMEPIAAKEMIIEYVGERIRQQVAQHREESYLKTGIGSSYLFRIDENTVIDATKKGGIARFINHCCSPSCTAKIIKVEGKKRIVIYALRDIEANEELTYDYKFERETNDEERIRCLCGAPGCKGYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEH7</accession>
    <name>G3AEH7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial Rho GTPase</fullName>
            <ecNumber evidence="15">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_47823</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 15">Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Mitochondrion outer membrane</location>
            <topology evidence="2 15">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the mitochondrial Rho GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="15"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372151.1">
        <property type="nucleotide sequence ID" value="XM_007372089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEH7"/>
    <dbReference type="FunCoup" id="G3AEH7">
        <property type="interactions" value="815"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEH7"/>
    <dbReference type="GeneID" id="18871149"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_47823"/>
    <dbReference type="eggNOG" id="KOG1707">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014255_3_0_1"/>
    <dbReference type="InParanoid" id="G3AEH7"/>
    <dbReference type="OMA" id="HETTWGI"/>
    <dbReference type="OrthoDB" id="10020961at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015886">
        <property type="term" value="P:heme transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055091">
        <property type="term" value="P:phospholipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010821">
        <property type="term" value="P:regulation of mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01892">
        <property type="entry name" value="Miro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000553">
        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013566">
        <property type="entry name" value="EF_hand_assoc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013567">
        <property type="entry name" value="EF_hand_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021181">
        <property type="entry name" value="Miro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020860">
        <property type="entry name" value="MIRO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08355">
        <property type="entry name" value="EF_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08356">
        <property type="entry name" value="EF_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037488">
        <property type="entry name" value="Mt_Rho_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51423">
        <property type="entry name" value="MIRO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="13 15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 15 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 15" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="650"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="Miro" evidence="18">
        <location>
            <begin position="3"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="226"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="348"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Miro" evidence="18">
        <location>
            <begin position="465"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR037488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW34739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="677" mass="76962" checksum="58A44C3D99FB7C00" modified="2011-11-16" version="1">MSQDTLRVVVCGDDSVGKSSLITSLIKETIIDSQPSVLPPITISQNDYIDSFDEYSLENSATRTKKNRSHQSNGDSVLSRFQESSSKMVSKYIPNITTIIDTSSSDMINLQKELKSADVIWLVYSDHYTYERISLHWMPMFRSMGVNIPIILCANKSDTQHKNTLKTQNQDEFVPLINEFKEIEAGIRCSAKNNYNVVEAFYLCQRAVTHPISPIFDAKEGNLKPLAIAALKRIFFLCDTDQDGYLNFKEFSDLHLKCFDHPATDLEYEDIMTKLSQKIYPDLSSGSEGISEDGFVLLNRMYAMSGRHETIWCILRAFHYTNSLSLHDKFLFPNLEVNPNSSVELSPSGYRFFVDLFIKFDKDNDGGLNEDELTNLFRPTPGIPKLWVETQFPASIVCNEEGYVTLQGWLAQWNLTTFLSYKTTLEYLAYLGFDEGTSVKALKITKPRKTRNRKGKFYRGNVNDRNVFNCFILGAPQSGKSSLLESFLHDSYSEMYSPTIQPRLVVKDIELRGGKQCYLILEELGELETAILENKQRLNQCDVICYTYDSSDPDSFQYIVDIRNKYAESLDEVPSVFAALKADLDKQQQRCDVQPENYTRQLNLSSPLHISSSWTSSLHELFIQLVDAAKNPASATPGLEKEQKVDQEQIIPIVMAGGAVAVMAMVSIWMFNSMHRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEN3</accession>
    <name>G3AEN3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pyruvate dehydrogenase E1 component subunit beta</fullName>
            <ecNumber evidence="10">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PDH1</name>
        <name evidence="12" type="ORF">SPAPADRAFT_58866</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="10"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373071.1">
        <property type="nucleotide sequence ID" value="XM_007373009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEN3"/>
    <dbReference type="FunCoup" id="G3AEN3">
        <property type="interactions" value="812"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEN3"/>
    <dbReference type="GeneID" id="18872653"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58866"/>
    <dbReference type="eggNOG" id="KOG0524">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012907_1_1_1"/>
    <dbReference type="InParanoid" id="G3AEN3"/>
    <dbReference type="OMA" id="WYANCPG"/>
    <dbReference type="OrthoDB" id="10266385at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07036">
        <property type="entry name" value="TPP_PYR_E1-PDHc-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000006">
        <property type="entry name" value="Pyruvate dehydrogenase E1 component subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027110">
        <property type="entry name" value="PDHB_mito-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006667">
        <property type="entry name" value="PRK09212.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008854">
        <property type="entry name" value="PRK11892.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624">
        <property type="entry name" value="DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624:SF96">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="9 10" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="11">
        <location>
            <begin position="63"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW35659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="390" mass="42126" checksum="A6F8580AF81E830C" modified="2011-11-16" version="1">MNPTAKKLTSSVAKTAQLAAQSLKYNKQRSALAATATAAAGQYQALRMMDMRSASSASGPQTMTVRDALNSGLAEELDRDDDVFLMGEEVAQYNGAYKVSRGLLDRFGERRVIDTPITEMGFTGLAVGAALHGLKPVLEFMTFNFAMQAIDQIVNSAAKTYYMSGGIQPCNITFRGPNGAAAGVGAQHSQCYAAWYGSIPGLKVLSPYSAEDYKGLIKASIRDPNPVIFLENEIAYGESFEMSEEALSSDFILPIGKANIEREGTDITIVGHSRAVKFAVEAAETLKKEYGVNAEVINLRSIKPLDVPTIIESVKKTNHLVTVENGFPAFGVGSEICAQIMESEAFDYLDAPVERVTGCEVPTPYARELEDFAFPDEPTILRACKKVLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEW6</accession>
    <name>G3AEW6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chromatin modification-related protein EAF1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Chromatin modification-related protein eaf1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14 16">ESA1-associated factor 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Vacuolar import and degradation protein 21</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">SPAPADRAFT_48757</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the NuA4 histone acetyltransferase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EAF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373208.1">
        <property type="nucleotide sequence ID" value="XM_007373146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEW6"/>
    <dbReference type="FunCoup" id="G3AEW6">
        <property type="interactions" value="242"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEW6"/>
    <dbReference type="GeneID" id="18871261"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48757"/>
    <dbReference type="eggNOG" id="ENOG502RGMX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006174_0_1_1"/>
    <dbReference type="InParanoid" id="G3AEW6"/>
    <dbReference type="OMA" id="HDWMLAR"/>
    <dbReference type="OrthoDB" id="5364245at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000484">
        <property type="entry name" value="Chromatin modification-related protein EAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46459:SF1">
        <property type="entry name" value="E1A-BINDING PROTEIN P400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46459">
        <property type="entry name" value="E1A-BINDING PROTEIN P400-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSA" evidence="19">
        <location>
            <begin position="212"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="18">
        <location>
            <begin position="461"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="543"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="602"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="674"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="831"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="943"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1024"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="607"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="624"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="676"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="717"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="734"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="784"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="837"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="943"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="956"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1026"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1044"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1070"/>
            <end position="1094"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00072841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00082479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EGW35796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1094" mass="124761" checksum="F1D5EA460A40C6A0" modified="2011-11-16" version="1">MASTWQARLSELVHVTTDPFLTKLLKADSHDDTKLMNLIQKSDNNAEQIFKDAIIKAMDESMFRPNIPREVIKRKSEAELDDGSRKFPKNSITLAREKKVSEVQSLKTQEEATLEALNEFLDKSINELEILSVPEHYPLKPQTVSSLAELYYLTQTLPLIKLLPASHKVLMTENYESALLEGKIAVLYSRIEELKRQGKWSLRQPQKFYDPFTYLKKTKKKTFYWDNVLQEARWMAEDFKQSARLKKWCCVTIAQAVEEYWVLGKQVCIKRKPIKHIEEKTAEEANTELAEDSAQIAVAEPETIDVSALNAEGDDESKKTVNDEIEITEFEPTPVVPQKQDSIFKVYLDHNDLKKIDQSIIQNLPKFTAFDDDESTYAPSKPLKPTEPPIAPVSRLLHPFEEDDSWYKIVLRDPGTEKNTQGPPEYQKGLFGFQAHRRFSTLKPPKPPLIKNIEFRSPTIWLPQDDKYLIHYVAEFCFNWDLISEHLSAHSSAASFKKYESNIERRTPWQCFERYIQLNEKFQFSDMKGVYAYPAQQWLEQAHRTQSTTKRRISPLGVGTDSIQRGHRRLRWASMFDAMRKCMKKREIALAKMNHRRSTTDYQGFNQSQQQTPTQTQPVRQSSEQPNSANQEVTPVKRSTDRVPTPGELSKLKFERDKSIQEAYLNQQATRSRMMAAVAQQQNQAKAGGSAPPQAPIAPGQAPRPIVSQPQPASGIPRAQQPVQARQPGPKSEGWNSPASMGSPQTNNMVLPQGQVPGMTRVGSQNGVPYQQQTAANPPPQAKRPTTPNGTPLTNEQIQQLLQLQKQRRLIQQQQQQQQQQQQQQQQQLQQQQQQQSAHSSQQPSPQLPMNTMMAQQPINSPNLQNMPINHQVPVAKPVQQSAQPPQQRTRIHFAPAQVSAIINSIQSKNPGFTKDQVTKLAAQYLASLQQQQQQQQQQQQQQQQQQQSIPSQQQTPTRYDQNSQRVAGQQRVQQMQPVAPGANNRNFNQSPNPRRIQAQSLTPEERQQLQMLKATGTSPLAQGYLQQKMPVQQQQQQQQKLQSASPITGSPNMSYSNKELEERRRILAQRQQADQSMQGPQSFSSGNGSPFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEX8</accession>
    <name>G3AEX8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LicD/FKTN/FKRP nucleotidyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_132572</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373220.1">
        <property type="nucleotide sequence ID" value="XM_007373158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEX8"/>
    <dbReference type="GeneID" id="18869670"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132572"/>
    <dbReference type="eggNOG" id="ENOG502QREF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008074_2_0_1"/>
    <dbReference type="InParanoid" id="G3AEX8"/>
    <dbReference type="OMA" id="MLNGVPC"/>
    <dbReference type="OrthoDB" id="444255at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009644">
        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF28">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE TRANSFERASE FKTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="6">
        <location>
            <begin position="408"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="728" mass="85577" checksum="BA51CF5C4F7FF4FA" modified="2011-11-16" version="1">MSKNISILIGTSKFYKPILFILLLNTLVISIYSSLRSRDLYAKEIYLNDISTLPQVSFTNSEISQKLMSLLAKFRAPTVTSEITVPTNYFDTDEIVQDPRIILSITLNWMYHQIKVDQKNISFPFNWADWVDLTYLNHQISKPENERIKCLDLIEHIHFNTPDDKAKSIADPMFFGCKNAHDLTKREMKALGLSDLDKMPGFFQFEHTAFKSSEFIRLLQGKTYLLSKMPIPYQVIFLNDNGNDLIFKVDSKMNGSELMKKYITNNNLGKQKDVTLDTIQEFKQIRSLLDVDTNEKMYNAKKILRMNKTWFHYGPDDVKNEITKLSTRPSLTPVEQGYLYSLITSKAASETPRQRELTYFQLATLITDDTNKDDGWHYDWRFINGKWRDRYRHALLLERLLRNWFKFCQKNGIVSWINFGSLLGWYRNGAIYPFDLDMDIQMPIHHMTILGRQFNQTLVVEDLREGTGKYLIDVGTYIHNRYTTRDYNHIDARFIDVDSGLYIDLTALSTSKDGETELYNDRHNWVYKFSDLSPLRLSLFEGIPFHVPKQIVKRMKFQYRCGTLNNFEFKHWYYVNQVGTWIHEKELLAVLDVKNYIEDGKVNKHKVKKQVENLTDEQLYTIISNDPATLSNYQLARRNFAFHTKEHQFLFKIDPKLHKNANDPPEGKVLDASPEENPEYIKLIEDNVWLRAPHRESICQFDKVKGKLIEYNQLAFRELDQITVSQEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFP9</accession>
    <name>G3AFP9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_48088</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372450.1">
        <property type="nucleotide sequence ID" value="XM_007372388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFP9"/>
    <dbReference type="GeneID" id="18871174"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48088"/>
    <dbReference type="eggNOG" id="ENOG502RJ98">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021057_0_0_1"/>
    <dbReference type="InParanoid" id="G3AFP9"/>
    <dbReference type="OrthoDB" id="4018341at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="411"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="413"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="461"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="762" mass="85917" checksum="661D6796F214A4E4" modified="2011-11-16" version="1">MSTPLAARRERRKVSRNRNSREGSPSDSHATTPEIADDQEFENDLDYLLTNKQKKKAEEYIETKVASEVIITGSWGEEKIAAATKKPTKQKNFTLADVTKDMEGLNFSSDSDEDNESLKKQSSTPSPSRKKKSKSKSKSKSKNNKVPSPPASQESNKIMSQEDQIKKEEDHATTILPSDSLVWSDDESITIPPAQIVPVIGSISKSAERARQRRQKKKLSKQINTLTEDEKEPTVSVVAKPEESKAFKITGEQFPELKVKESKKKKNSRPNSKGSKAITDDNHSKSDSKKKSKRLKSQDSSNGEIASKVDAKSAGKLTLNSLDQENHESEKLEKVLVMQLKMDGATLEIAEVEAMMVTTTKIMTTSHSMDAVKNTVENIAKDTMGDAMGDAMEDTVTVTTENEVAVAEAEAAAVDEEEVEEGEEEGEESTQSTNGHITREEKRRLRNVKRRQRRMKFKRAAAKEGKEGKEGKENKENKENKENKENKEVKEVSDLSTEPLPNIKKLQILQREPSIETTPVPATTNPFAVKLRPVNISTNKKYSETLPEIHPIKTIIGITHKQILNSDLSDVKIKTLISSSSVSKYASTVVKFTTAHSEDLFGDEDLKRIFMCLCLNVALFESLGFRKTRKQFANILELFGGYDEINLETTRTKMTPSTKMIHQNNLDYSVLSYVGHILIWAVNQQRQAGVPLFTEKFDLDLSQAMIKENIGGYHLWDRLHREAKSMNSKRWKHIIKFRTAFPYEEDQFMIILRFMKISDDIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFS3</accession>
    <name>G3AFS3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Coatomer subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">SPAPADRAFT_132247</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Oligomeric complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus membrane</location>
            <topology evidence="1 11">Peripheral membrane protein</topology>
            <orientation evidence="1 11">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasmic vesicle</location>
            <location evidence="11">COPI-coated vesicle membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the COPG family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372474.1">
        <property type="nucleotide sequence ID" value="XM_007372412.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFS3"/>
    <dbReference type="FunCoup" id="G3AFS3">
        <property type="interactions" value="1262"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFS3"/>
    <dbReference type="GeneID" id="18869647"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132247"/>
    <dbReference type="eggNOG" id="KOG1078">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010353_2_0_1"/>
    <dbReference type="InParanoid" id="G3AFS3"/>
    <dbReference type="OMA" id="DFIEDCE"/>
    <dbReference type="OrthoDB" id="1074925at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000368">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1480:FF:000001">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1480">
        <property type="entry name" value="Coatomer, gamma subunit, appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009028">
        <property type="entry name" value="Coatomer/calthrin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032154">
        <property type="entry name" value="Coatomer_g_Cpla"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017106">
        <property type="entry name" value="Coatomer_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013040">
        <property type="entry name" value="Coatomer_gsu_app_Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037067">
        <property type="entry name" value="Coatomer_gsu_app_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261:SF0">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16381">
        <property type="entry name" value="Coatomer_g_Cpla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08752">
        <property type="entry name" value="COP-gamma_platf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037093">
        <property type="entry name" value="Coatomer_gamma_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55711">
        <property type="entry name" value="Subdomain of clathrin and coatomer appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 11" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="12">
        <location>
            <begin position="22"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer gamma subunit appendage Ig-like subdomain" evidence="13">
        <location>
            <begin position="671"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer subunit gamma C-terminal" evidence="14">
        <location>
            <begin position="827"/>
            <end position="937"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR037093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EGW35062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="938" mass="104959" checksum="6A5BE7C1A53F9062" modified="2011-11-16" version="1">MSTSSYKKQDPYSSTAGLPDKMAVFQECLQQFNATPVNAKKCRQLLAKLLRLVYHGETFPSQESTTLFFSISKLFQHKDVSLRQLVYLTIKELSSTSDDILMVTSSIMKDIQGNDAVYKPNAIRTLSKVLDSTTVHSAERLFKNAIVDRNPVVSSAALISAYNLLPSSKDVVKRFTNETLETIQSYKQFPPTQFQLHEYYGSATSNLPSTSYMYQYHALGLIYQLRSNDKMALMKLITSLSEGSSLKNSLSIIQLIRYINKILLDDSSLIGHLFPILSGFLKHKSDMVELEACKTLVSLQHLIRDDQFMAIVTTLQKLLSVPRTATRFAAIRLINKISIKHPEKIMVVNIELESLINDSNRSISTLAITTLLKIMGAGTIDNGSGGSESVDRLIAKMTSLMDEITEDFKIVIIEAIENLALKFPSKHKKLVTFLTDLLRDDGSLQLKTSIVDALFDLIKFLPDPSAKQLMLMNLCEFIEDCEYTELSVRILHWLGDEGPNTSNPSYYIRHIYNRLVLENSIVRSSAVIALAKFAAVCGGDVAKNIIILLGRCLNDVDDEVRDRAAISLKFINSNKKKLIVSDSRYDLYTLENKLVHYLNDEENFNEKFDIDEVPVISNEEYNSIEYNRKINKLENINDNSAVTEVEERVKSPTGGEATPGDSLANDLLKQQEYSQQLSGVEQFVEYGKLTKSTIEPVYLSERESEIAVSVVKHLFIESKHLVLQYNINNTLPNTVLQDISVVAQPDNDLYQEDFIIPLAELRPNDTGIVYVSFTIPDIEEAELLSAFGNTIAYTSRDVDEDGQVDPNDDGGWPDEYQIDDLEVLACDFITPLYNSNFTALFDQLPYQDTSVVTINQVDNLEKAIEKLKNSLNMMPLDGSDFVSSGEAAHSLKLLGKDVWGGKVGASIRLASTGGKIVAKVEVKSETENFGNIVISSVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFU4</accession>
    <name>G3AFU4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HAD-superfamily hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58223</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372495.1">
        <property type="nucleotide sequence ID" value="XM_007372433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFU4"/>
    <dbReference type="FunCoup" id="G3AFU4">
        <property type="interactions" value="263"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFU4"/>
    <dbReference type="GeneID" id="18872370"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58223"/>
    <dbReference type="eggNOG" id="KOG1618">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030880_1_0_1"/>
    <dbReference type="InParanoid" id="G3AFU4"/>
    <dbReference type="OMA" id="WPFHDLT"/>
    <dbReference type="OrthoDB" id="10251048at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050324">
        <property type="entry name" value="CDP-alcohol_PTase-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006353">
        <property type="entry name" value="HAD-SF_hydro_IIA_CECR5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01456">
        <property type="entry name" value="CECR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269:SF57">
        <property type="entry name" value="SUPERFAMILY HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G02580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="42232" checksum="5442BA09E858C6E2" modified="2011-11-16" version="1">MILNRLRLPTVSKLSQLAFARNYSRKDFAFVFDIDGVLIRGKDPIAAATPALELLNEHRVPYILMTNGGGVSEKAKADHIAKIVGVPISPWQIVQSHTPLRTLANDRKHNRVLCIGGVGDAVRHVAKSYGFEDVVLPIDIVKAIPSISPHHMFKQDDFDKYAIDIDVNVPFRAILVFNDPRDMSTDVQIVQDLLNSQDGVLGTKRKRRRLEDPAIPIVFSNSDVYYSNEYSLPRFGQGAFRIITEELYSHINQLRPHEAMSSLLCGKPFKLQYDFAHHVLIDWREMLITNKFEQPKYPLLGTAPTASPFKNIYMVGDNPASDIQGANDHGWESVLLRTGVYADEDLSSLEAQPTAGVFNDVYDAVLEILRVNKVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFV2</accession>
    <name>G3AFV2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_48138</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372504.1">
        <property type="nucleotide sequence ID" value="XM_007372442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFV2"/>
    <dbReference type="FunCoup" id="G3AFV2">
        <property type="interactions" value="748"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFV2"/>
    <dbReference type="GeneID" id="18871185"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_48138"/>
    <dbReference type="eggNOG" id="KOG3297">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073901_1_0_1"/>
    <dbReference type="InParanoid" id="G3AFV2"/>
    <dbReference type="OMA" id="LGPTLWW"/>
    <dbReference type="OrthoDB" id="1937997at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04330">
        <property type="entry name" value="RNAP_III_Rpc25_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000221">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.120:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit RPC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013238">
        <property type="entry name" value="RNA_pol_III_Rbc25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004519">
        <property type="entry name" value="RNAP_E/RPC8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00448">
        <property type="entry name" value="rpoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08292">
        <property type="entry name" value="RNA_pol_Rbc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb7-like N-terminal" evidence="7">
        <location>
            <begin position="8"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase III subunit Rpc25" evidence="8">
        <location>
            <begin position="83"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW35092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="196" mass="22448" checksum="BDB3E8292D207245" modified="2011-11-16" version="1">MFVLTEITDLVRIPPHTFNIPIQHSITDELNKKLANKVIANLGLVITIWDLLDIKDGLLKPGDGGSYVEVQFRCVVWKPFVGEVLTGWVSECTPQGIKIRLEFFDEIFIPRAYLFEGCEFKPVEKAWVWKPDEETELFIDINEKIRFRVEQEIFVNIKPKSSNEAVGLEEPTNKAPPYAIMASCQTEGMGCVSWWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AFZ2</accession>
    <name>G3AFZ2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">KRR1 small subunit processome component</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">KRR-R motif-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_58320</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly. Essential for vegetative growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Interacts with snoRNA U3. Interacts with MPP10, KRI1 and with ribosomal proteins RPS1A, RPS4A, RPS4B, RPS8A, RPS8B, RPS11A, RPS11B, RPS13, RPS24, RPS25, RPL4A, RPL7B, RPL8, RPL23, RPL25 and RPL28.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
            <location evidence="1 11">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the KRR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372543.1">
        <property type="nucleotide sequence ID" value="XM_007372481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AFZ2"/>
    <dbReference type="FunCoup" id="G3AFZ2">
        <property type="interactions" value="1156"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AFZ2"/>
    <dbReference type="GeneID" id="18872398"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58320"/>
    <dbReference type="eggNOG" id="KOG2874">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040185_0_2_1"/>
    <dbReference type="InParanoid" id="G3AFZ2"/>
    <dbReference type="OMA" id="TPDIDKW"/>
    <dbReference type="OrthoDB" id="441223at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22393">
        <property type="entry name" value="KH-I_KRR1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22394">
        <property type="entry name" value="KH-I_KRR1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000011">
        <property type="entry name" value="KRR1 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000014">
        <property type="entry name" value="KRR1 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041174">
        <property type="entry name" value="KRR1-like_KH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048550">
        <property type="entry name" value="KRR1-like_KH1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048548">
        <property type="entry name" value="KRR1-like_KH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048549">
        <property type="entry name" value="KRR1-like_KH2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024166">
        <property type="entry name" value="rRNA_assembly_KRR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581">
        <property type="entry name" value="HIV-1 REV BINDING PROTEIN 2, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581:SF0">
        <property type="entry name" value="KRR1 SMALL SUBUNIT PROCESSOME COMPONENT HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17903">
        <property type="entry name" value="KH_KRR1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21800">
        <property type="entry name" value="KH_KRR1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006515">
        <property type="entry name" value="KRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 11" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="6 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 11" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="K Homology" evidence="13">
        <location>
            <begin position="122"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="278"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="278"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="320"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW35131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="351" mass="41357" checksum="7DAD04000B5ABE5B" modified="2011-11-16" version="1">MPSTHNRDKPWDTPDIDKWVIDEFKPEDNASGLHFAEESSFMTLFPKYREEYLRNIWSQVTQALDKHFIKCELNLIEGSMTVKTTPKTFDPAMILKARDLIKLLARSVPFGQAVKILQDDIACDVIKIGNFVNNKDRFIKRRQRLVGPNGNTLKALELLTKCYILVQGNTVSAMGPFKGLKEVRRVVEDCMRNIHPIYYIKELMIKQELSKKPELANEDWSRFLPSFKKRNVVRKKKKMANKEKKVYTPFPPAQQPRKIDLQIESGEYFLGKKERELKKLQEKRDKQEEVSESRKQERMKDFEAPTEEKHENKLVEKKDKKDKKDKKDKKDKKKRHADDEEESGKHKKSKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG43</accession>
    <name>G3AG43_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein SMI1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">SMI1</name>
        <name evidence="4" type="ORF">SPAPADRAFT_58375</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the KNR4/SMI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372594.1">
        <property type="nucleotide sequence ID" value="XM_007372532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG43"/>
    <dbReference type="STRING" id="619300.G3AG43"/>
    <dbReference type="GeneID" id="18872423"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58375"/>
    <dbReference type="eggNOG" id="ENOG502QTAZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027501_1_0_1"/>
    <dbReference type="InParanoid" id="G3AG43"/>
    <dbReference type="OMA" id="HIGNQIA"/>
    <dbReference type="OrthoDB" id="2305498at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070880">
        <property type="term" value="P:fungal-type cell wall beta-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1580.10">
        <property type="entry name" value="SMI1/KNR4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009203">
        <property type="entry name" value="Knr4/Smi1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018958">
        <property type="entry name" value="Knr4/Smi1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037883">
        <property type="entry name" value="Knr4/Smi1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051873">
        <property type="entry name" value="KNR4/SMI1_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47432">
        <property type="entry name" value="CELL WALL ASSEMBLY REGULATOR SMI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47432:SF1">
        <property type="entry name" value="CELL WALL ASSEMBLY REGULATOR SMI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09346">
        <property type="entry name" value="SMI1_KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017023">
        <property type="entry name" value="KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00860">
        <property type="entry name" value="SMI1_KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160631">
        <property type="entry name" value="SMI1/KNR4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Knr4/Smi1-like" evidence="3">
        <location>
            <begin position="129"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="203"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="386"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="210"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="397"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="458"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="491"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="501" mass="56043" checksum="80B6F68B6A402015" modified="2011-11-16" version="1">MGLFQEFKAFIHSITTDDHYASYDSRYRNGSSGGAPGLSRGDSGLVNNGSTSRLNEISRLNGSGHSFEQAAAAAKIGYRPGLRSQQDLPLQTFDENGQPPLPSIDSLWDRIERWMEEEYPELEDSLNDGVTTTDLNEFENDLGCGNLPEDFRQFYKRHDGQLRGGRPTGLFMGLALLDLEGIIEENAIWLKVAERLERQQYMVQHQQKEGTSSTASSRGSSVNNSFIGNQRSIPPNSIQPFYYQKGWITLMKDHIGNQIALDLAPGPQGKWGQIIIFGRDFDTKLVIANSLTEFIFNFVTDLEQGNYQIDSSVVQEELGYLGRERNDDYMIGDEDEDEGELFFYDRDNKEFGKGARGKLSYIEVFKRRTLKKYGLTENFSTQFTPQVKPTASAAPKSGTSSPLLNQSRNSSASNLKPGSTKPATSSPLVNSGQFSVPKETIIDDKTTAKSTPEIMVDDTDKTPEPEIEESKPEEVVKSKEESKEDLKEELKEEDQDVNNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG50</accession>
    <name>G3AG50_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58385</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372601.1">
        <property type="nucleotide sequence ID" value="XM_007372539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG50"/>
    <dbReference type="GeneID" id="18872429"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58385"/>
    <dbReference type="eggNOG" id="ENOG502RQKJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1489886_0_0_1"/>
    <dbReference type="InParanoid" id="G3AG50"/>
    <dbReference type="OMA" id="NMCRERS"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="181" mass="21254" checksum="9F33C7449EF9274D" modified="2011-11-16" version="1">MDLDYYESLGYNESEEVLIKVKKDILNSLELALWTYENKYDIDEDIIEYCVSSFVIHDYINEKTKDILKIHDPVTLCSYNKIKQSSLNKLLEINNSAVTQVRIGQAAIKYGFKEIFLKLKFRTDVALFNMCTTYETKVFHDIMMEHIYKGEKVTDFLNYGCITYENEEMDDLVKGIEDSMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AG70</accession>
    <name>G3AG70_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pyridoxal phosphate homeostasis protein</fullName>
            <shortName evidence="2">PLP homeostasis protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_133045</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Pyridoxal 5'-phosphate (PLP)-binding protein, which may be involved in intracellular homeostatic regulation of pyridoxal 5'-phosphate (PLP), the active form of vitamin B6.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the pyridoxal phosphate-binding protein YggS/PROSC family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372621.1">
        <property type="nucleotide sequence ID" value="XM_007372559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AG70"/>
    <dbReference type="FunCoup" id="G3AG70">
        <property type="interactions" value="497"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AG70"/>
    <dbReference type="GeneID" id="18869713"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_133045"/>
    <dbReference type="eggNOG" id="KOG3157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059988_2_0_1"/>
    <dbReference type="InParanoid" id="G3AG70"/>
    <dbReference type="OMA" id="PLEWHMI"/>
    <dbReference type="OrthoDB" id="10264196at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042816">
        <property type="term" value="P:vitamin B6 metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06822">
        <property type="entry name" value="PLPDE_III_YBL036c_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000007">
        <property type="entry name" value="Pyridoxal phosphate homeostasis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02087">
        <property type="entry name" value="PLP_homeostasis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011078">
        <property type="entry name" value="PyrdxlP_homeostasis"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00044">
        <property type="entry name" value="YggS family pyridoxal phosphate-dependent enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10146">
        <property type="entry name" value="PROLINE SYNTHETASE CO-TRANSCRIBED BACTERIAL HOMOLOG PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10146:SF14">
        <property type="entry name" value="PYRIDOXAL PHOSPHATE HOMEOSTASIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004848">
        <property type="entry name" value="YBL036c_PLPDEIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alanine racemase N-terminal" evidence="5">
        <location>
            <begin position="14"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2 3">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR004848-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW35209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="246" mass="27451" checksum="41E2657972ABD8C9" modified="2011-11-16" version="1">MSTFTEATEARKSELSENYRAIIDKINQLNPKVNLVAVSKIKPSSDIKALYDAGVRHFGENYVQELIAKSQELPKDIKWHFIGGLQSGKAKDLAKHVESLYAVETIDSLKKCKQLDNTREKVGGSEIKVFLQINTSCEEQKSGYNSEVLDDLEETVKYLLSDECKKLKLIGLMTIGSFSESTSESGENQDFKKLVEVKEILDKKYQLDLELSMGMSNDFEQAIKQGSTSVRVGSSIFGARPPRNAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGH0</accession>
    <name>G3AGH0_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_58531</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372721.1">
        <property type="nucleotide sequence ID" value="XM_007372659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGH0"/>
    <dbReference type="STRING" id="619300.G3AGH0"/>
    <dbReference type="GeneID" id="18872490"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58531"/>
    <dbReference type="eggNOG" id="ENOG502RPYU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1126862_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGH0"/>
    <dbReference type="OrthoDB" id="4082978at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW35309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="247" mass="28319" checksum="C13C41286BC5E292" modified="2011-11-16" version="1" fragment="single">MVTQLPIKRLYDPKTKKLRKSIVFPRVNEVAKTHPSSYQEKITVKIKMTGINYELDFIDDCDVVVPGKGMVVKISSIPPSIESVLAYNPKQKYLVFPYTTCKLENQHLEESCECHLVYGKDLDGGLQEFLDVPHQLLIPIPQNISIHDVCFVTEILLPFYNNLNKVHLNSANSKTLILLNDIRKEINEILIIIKHFNIPQSSIVFLDNYTISKLKYPSMYSGRFQTVFCFNKSLQKFAEFSCISTGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGL7</accession>
    <name>G3AGL7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Trafficking protein particle complex II-specific subunit 65 IgD3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_132432</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372768.1">
        <property type="nucleotide sequence ID" value="XM_007372706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGL7"/>
    <dbReference type="FunCoup" id="G3AGL7">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGL7"/>
    <dbReference type="GeneID" id="18869659"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_132432"/>
    <dbReference type="eggNOG" id="ENOG502RYDF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_490882_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGL7"/>
    <dbReference type="OMA" id="WSPYLDF"/>
    <dbReference type="OrthoDB" id="5345392at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055420">
        <property type="entry name" value="IgD3_Trs65"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024662">
        <property type="entry name" value="Trs65"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28159">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX II-SPECIFIC SUBUNIT 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28159:SF1">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX II-SPECIFIC SUBUNIT 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12735">
        <property type="entry name" value="IgD3_Trs65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trafficking protein particle complex II-specific subunit 65 IgD3" evidence="1">
        <location>
            <begin position="436"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="599" mass="67029" checksum="B933C60FCDE0014D" modified="2011-11-16" version="1">MSIEVKLPSQPLDLEKDVISQLESSDPRKIVFFDESITGYVVLTAEGETRPPPKRVSLAMTILPSEVVDTHDPLHYLESVDQRHKFSIVDLVLDNIVYSGKGSSNKQYYVWSFEIPVKYPRKRLNDPRLFLSCMLIEGDQNEEKSNNSSYTEADTLHDYSTSSIDKILPIKLSESLCDIEISHTTTDFDITIENESDNTYIYKPENSIRGYISVPVSISLVIKLKSTKPAGRNDILLTTFTIESSDELLKFWNEDEDDHYFNLVDVNLQFKSGSIDPIIGNDLMPPTRFKYRDTISLAYKLTNNEPLAENVHSSKPVHIFLMLQVQKRHNGEFYNISNTIHTEWTPYLDFGLIASPINSTLKSVSSSLQTYLQPNAPLNTSIRKPTLLQSMYKGPNLNNSSANIAMTNTISNKNPQHQLQKSRTFSSGSIMVSLSTNANSTLSGLKLSFIGKLNIKLGEVITWKIQAINHASTRLNLSLLVQNPINFNVIYSNSAPHHKNASTHSVSNNDNKAHNAAIVHDKSNLYSTYNSLKVNNNSGVVILGNDIRIGPLDSNTAFETELQLMGISKGIFNLDGVKIFDINSGDGLDFGKLIEVFVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGN5</accession>
    <name>G3AGN5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Short-chain alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_58595</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372786.1">
        <property type="nucleotide sequence ID" value="XM_007372724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGN5"/>
    <dbReference type="GeneID" id="18872523"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58595"/>
    <dbReference type="eggNOG" id="KOG1208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_6_1"/>
    <dbReference type="InParanoid" id="G3AGN5"/>
    <dbReference type="OMA" id="GTSKAFW"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF236">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW35374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="335" mass="37884" checksum="65C518758896761C" modified="2011-11-16" version="1">MDSPIIPKLTWQEWKQVLHGFHPGNPTFLEKDYPSLDGKVVIVTGGSTGVGYQVVKSLAGSTNAKIYIFSRNQQKARDAIERMKLEIAEEYSKAVDESIKFVQVDLSDLTTIKPAVSEFLQAEDRLDIIIHNAGIMMPPRGSVTKQGYQMELGTNNIGPQLLQNLLDPIFIKTSSTNKPNESRIVWIASTSHFFSPLHGINWNDPNYRQVTNVPLKQMYSQSKAINIIQAQQWPVYHPEATNAVSISVCPGYLKTELQRNTTWLQQLGYSLLFHNPRYGAYTELFAALSPDITSANNGQHVISFGHFGEIRKDLEEKENSNKVWNFLQDQINQYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGQ4</accession>
    <name>G3AGQ4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_131954</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372799.1">
        <property type="nucleotide sequence ID" value="XM_007372737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGQ4"/>
    <dbReference type="STRING" id="619300.G3AGQ4"/>
    <dbReference type="GeneID" id="18869623"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_131954"/>
    <dbReference type="eggNOG" id="ENOG502QVYJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007695_0_0_1"/>
    <dbReference type="InParanoid" id="G3AGQ4"/>
    <dbReference type="OMA" id="QQHRQWT"/>
    <dbReference type="OrthoDB" id="3364175at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051127">
        <property type="entry name" value="Fungal_SecMet_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF6">
        <property type="entry name" value="PROLINE UTILIZATION TRANS-ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="477"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="548"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="17"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW35387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="710" mass="81629" checksum="4EF73CF423DC6AC5" modified="2011-11-16" version="1">MSEPEDEGVKRTKVSRACDYCKKRKFKCSGKSPCNLCTKKKLECVFSIIDRRTIRRKNKKRKIKKEVVKPVIDNQTLNLLTQKANIPSHYQPLLIFPLHNGINNANDITGGHKLMVKPEDPPKLIFDSQGNLRYFGETSPLSLLFECRAIFKDKLGESEFTTQGTCPIVDEPEESEDVVAPPPLPTRETFNRIFEVFLININQVCYVFDTNYFTKNTVEFVYADPTCANCPRDKISLINLVIGVGLKYAELCQDPIVAEFTHSAANYMEFGLDLANRYINKNKVWVSEAYSMAYYYYQAVQQRNTAWLMLGTAVRNAQALGLHRKFINDSFDDPGYRRHRRKLFKSLYVNDYIVSLLHGRPLHIDDYDYDDFDNEDIFEIDRDGNQIKSQKLLCLIETTKLYRITGKIIKNCYSHSDKMNGIIAEKLAIELKLWSVNLPPCIQIDKVVENTDSRTHHSIDTIRDAKIPLLLVHLSQLYAIILLCRPFFMYIIAVKAGKKKASKRMKSIEEIAICNFGKAAVKSSILVIQLVDYYVGYIQFLPMRVESYGVVHATFNAALIIGMSMLYYELNGSYDDPDYSRINSMGYLNTAKQVFQFYSDRNIMSQRFYDSIESMQSALMGKFGFDIHGNDKEVKQEVKQEVLKKFPMFNYDDFIDNFGALLPMTTMGQSVDQYLSESSTTSSLFENSVSQPLDMFMYGMGEININTLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AGV6</accession>
    <name>G3AGV6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-isopropylmalate synthase</fullName>
            <ecNumber evidence="4">2.3.3.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LEU4</name>
        <name evidence="11" type="ORF">SPAPADRAFT_57675</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.13" evidence="4"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372041.1">
        <property type="nucleotide sequence ID" value="XM_007371979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AGV6"/>
    <dbReference type="FunCoup" id="G3AGV6">
        <property type="interactions" value="205"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AGV6"/>
    <dbReference type="GeneID" id="18872146"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57675"/>
    <dbReference type="eggNOG" id="KOG2367">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004588_3_0_1"/>
    <dbReference type="InParanoid" id="G3AGV6"/>
    <dbReference type="OMA" id="WPDKVID"/>
    <dbReference type="OrthoDB" id="418791at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07942">
        <property type="entry name" value="DRE_TIM_LeuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000045">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00572">
        <property type="entry name" value="LeuA_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005668">
        <property type="entry name" value="IPM_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054692">
        <property type="entry name" value="LeuA-like_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039371">
        <property type="entry name" value="LeuA_N_DRE-TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00970">
        <property type="entry name" value="leuA_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002991">
        <property type="entry name" value="PRK03739.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46911">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46911:SF2">
        <property type="entry name" value="2-ISOPROPYLMALATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22615">
        <property type="entry name" value="IPMS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89000">
        <property type="entry name" value="post-HMGL domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="10">
        <location>
            <begin position="58"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EGW34629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="594" mass="65830" checksum="E786AC91E1339B9C" modified="2011-11-16" version="1">MRINFRVFIRTLSSGGRLPPVKLTYQNMLKDPSIKYPPPKHINLPNRTWPNKVITKAPRWLSTDLRDGNQSLPDPMSVAEKKEYFHKLVEIGFKEIEVSFPSASQTDFDFTRYAVEHAPEDVSIQVLTQSREPLVRRTVESVKGAKKAIIHIYLATSDVFRDVVFGMSKEDAISKAVETTKLVRSLTKDDPSMNETEWNLEFSPECFSDTPVEFAVEICEAVKKAWEPTAEHPMIFNLPATVEVAGPNVYADQIEYFCTHITEREKVVVSTHCHNDRGCGVAATELGILAGADRVEGCIFGNGERTGNVDLVTVALNMYTQGIAPELDFSDLESVIEVSERCNKINVHPRAPYGGSLVVCAFSGSHQDAIKKGFSKAEERVKKGDIRWAMPYLPLDPKDIGRNYEAVIRVNSQSGKGGASWVILRSLGLDLPRHLQVAFSTLVQHKADSLGRELLTEEICQLFTNEYLHKSDPFHLVDFNITKVKDDREIKAVLASSTGEVTISGQGNGPISAFVDALSNKFGILFEVVNYSEHSLGSGSSSKAATYVELSYLNSNDKRVSKWGCGIHTDVSQASIEAILSVVNSLIDSKEISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3AH75</accession>
    <name>G3AH75_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_58744</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372917.1">
        <property type="nucleotide sequence ID" value="XM_007372855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AH75"/>
    <dbReference type="GeneID" id="18872584"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58744"/>
    <dbReference type="eggNOG" id="ENOG502T27P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_947017_0_0_1"/>
    <dbReference type="InParanoid" id="G3AH75"/>
    <dbReference type="OMA" id="LVYICFD"/>
    <dbReference type="OrthoDB" id="4013248at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442312" description="GOLD domain-containing protein" evidence="3">
        <location>
            <begin position="19"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="221"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="4">
        <location>
            <begin position="18"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW35505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="256" mass="29931" checksum="DFB031E806F9563A" modified="2011-11-16" version="1">MLSIFILIFLFITTYVSALGISVSPVKYGQSKQMSSISNLHHCVTYETNVNDIIFVTVKTEEKLPSQILNLNIFDSDSNQLRNQKDIGQREIELIISHANNKYREVKSSKRGEAAKKTINICFDNVYTDMSWSFTPADYEVELFVDIKKDMKDTNYDLYRKYFSKSRKQTEKFTQENLDTNMEEVKQELNAIVEKLESSESILRDLMDQEYKLRDVNEEIYSGYTIISIVVLSFIVFSGLIQLIYFKCYLKKRKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3AHP4</accession>
    <name>G3AHP4_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59641</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373792.1">
        <property type="nucleotide sequence ID" value="XM_007373730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AHP4"/>
    <dbReference type="GeneID" id="18872999"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59641"/>
    <dbReference type="HOGENOM" id="CLU_199937_0_0_1"/>
    <dbReference type="InParanoid" id="G3AHP4"/>
    <dbReference type="OMA" id="FDEKQEY"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW34208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="60" mass="6815" checksum="AF4818E3CBDC00A8" modified="2011-11-16" version="1">MSLFGTKKKESSRRSSVASNSSSSCKESFASFDEKKDYVRRCSLKKHKSKPQPYTFCLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI15</accession>
    <name>G3AI15_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATPase synthesis protein 25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_59743</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Mitochondrial mRNA stabilization factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion inner membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
            <orientation evidence="1 7">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ATP25 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373913.1">
        <property type="nucleotide sequence ID" value="XM_007373851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI15"/>
    <dbReference type="STRING" id="619300.G3AI15"/>
    <dbReference type="GeneID" id="18873060"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59743"/>
    <dbReference type="eggNOG" id="ENOG502RGZN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_454918_0_0_1"/>
    <dbReference type="InParanoid" id="G3AI15"/>
    <dbReference type="OMA" id="WLREQMM"/>
    <dbReference type="OrthoDB" id="107372at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140053">
        <property type="term" value="P:mitochondrial gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048255">
        <property type="term" value="P:mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040152">
        <property type="entry name" value="Atp25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28087">
        <property type="entry name" value="ATPASE SYNTHESIS PROTEIN 25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28087:SF1">
        <property type="entry name" value="ATPASE SYNTHESIS PROTEIN 25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02410">
        <property type="entry name" value="RsfS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="23"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW34329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="590" mass="68471" checksum="9B2307635A34DC18" modified="2011-11-16" version="1">MIPRSKLVSRAYLNHIRSSTLVPRSHRRNLQSETPINTDKVGEEQKENLENETANVSETVEHDSSIPWYLREDASSQIRTVEVELPEVPEHAPATVADFLHVVGQEYGMSDIKLYDLSELPEDHTYSVTNQPFKYIIICSGKSEKHIYKAASELRYRIKHERGYLPSMEGRVSSALNALARRRLMKRAGKGPAATDNEFGLGKNSWVMCDTGVDGIVVHMLTPDRREHLKLESLWSEQEDNVSTESSIDSDSIFRGFRRRFHTSTRCFNGNKLKEIYDLLLVQESPKSLQSLKQEFDLSFKGLNIQEYNIKYDFYKSLHLLDPEAVTEPDLLRIVWDKYSSLSLVCDNWKQEITHDVIKYMELLVDSPIASHEEPAEKYNKLSKFISQITQFSNEEVNLLSNPQFQSLLWRLSYNGSNEVDAATVSEIIQTGQPTETTYSEYLVLDEKKSRDAWTFIEQCYHKSGTPVPVWFREQKMYTYGNCGKWTEFWNEWDVIVQTVPTEHVLGYWVKLLVYLTERNTIEELRNFFVTYWNQPQRMGYSFIADYIKHGEQFTTKEEKHAIKSAINKLASECSTFPWFQPASDFASKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AI36</accession>
    <name>G3AI36_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_59761</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373934.1">
        <property type="nucleotide sequence ID" value="XM_007373872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AI36"/>
    <dbReference type="GeneID" id="18873069"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59761"/>
    <dbReference type="eggNOG" id="ENOG502S90H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_744170_0_0_1"/>
    <dbReference type="InParanoid" id="G3AI36"/>
    <dbReference type="OMA" id="QGYWRCL"/>
    <dbReference type="OrthoDB" id="4044171at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035187">
        <property type="entry name" value="Mpm1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17234">
        <property type="entry name" value="MPM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW34350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="355" mass="42240" checksum="DC866F96A9FE2C37" modified="2011-11-16" version="1">MCGKDKDCDRSCDCHNKNKHPWNPSWNAPWTEEEHDRFHRHVDNVKDNLKSLTNTMLDVSSDLGKDLNERARRWSLKWLNNDDDDEKFNSFFGDKKFDKIQYEDNGKFEFPSFYQARDVIQDVTQHPIFGEIWNAIPGFGSFRDGRTPFGYYAYKGPSMRQYHNCLEKNGKSIWDDKGYWRCLFPNGEIPVDMLNYKKKYLYGEILTKEDFYDAMKELSVNEKEPTIDLREKGTFFNKFDDYLNWKHVKYSLKQVEQQRRIAEQQKLLEERKKQAELAPKGQTVDKRVVSTSVKSHMYSDSETNETKMIEEKVECFNDGTCTTTKVTKSRPFDSNEWVTVEETHEDKDKNGWFWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIB0</accession>
    <name>G3AIB0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Derlin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_59048</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in the degradation of misfolded endoplasmic reticulum (ER) luminal proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the derlin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373263.1">
        <property type="nucleotide sequence ID" value="XM_007373201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIB0"/>
    <dbReference type="FunCoup" id="G3AIB0">
        <property type="interactions" value="487"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIB0"/>
    <dbReference type="GeneID" id="18872741"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59048"/>
    <dbReference type="eggNOG" id="KOG0858">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_760753_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIB0"/>
    <dbReference type="OMA" id="PDCLWFT"/>
    <dbReference type="OrthoDB" id="19102at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007599">
        <property type="entry name" value="DER1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11009">
        <property type="entry name" value="DER1-LIKE PROTEIN, DERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04511">
        <property type="entry name" value="DER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="362" mass="40502" checksum="0DB1983C14552EA5" modified="2011-11-16" version="1">MSNTIVENIKSIPPVTRFFTIASIFACLIMSTLGTQASFHRLFINYYTIIYDYDTIIRPVLSNANAKYYQIVYAVLEFAAQVYKFGTSLFIPAGINDSTNRIQAIFDIYFFYTFSNHLEGYEGKFKGNFPDYLWYIIICGTLTHIITIIYTVFYTEIFFPHEILLGCVTYTWSRCFKNATINLLGIVPIKAYYLPLGNLFVKLILSGPHAMISTLMGIVVGYLYLCIQSNTLPIYNLFPGAYGTPGPNKKREGSRLGVTHIDQPTRGSTYDFIGDSIYDKGYLKAPEVLYKWLSYPVESSFRSTAFTDFKGNTLNGRATDATVRRASAAQEVASGSSSGYSWFGNNGTSGASFKGKGHRLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIJ1</accession>
    <name>G3AIJ1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_54012</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the thioredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373290.1">
        <property type="nucleotide sequence ID" value="XM_007373228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIJ1"/>
    <dbReference type="FunCoup" id="G3AIJ1">
        <property type="interactions" value="624"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIJ1"/>
    <dbReference type="GeneID" id="18871828"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54012"/>
    <dbReference type="eggNOG" id="KOG0907">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_14_0_1"/>
    <dbReference type="InParanoid" id="G3AIJ1"/>
    <dbReference type="OMA" id="DFHALWC"/>
    <dbReference type="OrthoDB" id="10263751at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000245">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46115">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <feature type="site" description="Deprotonates C-terminal active site Cys" evidence="3">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to redox potential value" evidence="3">
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to redox potential value" evidence="3">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="4">
        <location>
            <begin position="30"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW33706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="103" mass="11357" checksum="B3B980F32B52DFA5" modified="2011-11-16" version="1">MVSAVSSTQEFQDALKYEGLVVVDFFATWCGPCKMIAPLLDKFSSEYPQVKFLKVDVDQFGSIAQEYEVTSMPTIIFFKNGQQIQRVIGANPAALKNAIATNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIL6</accession>
    <name>G3AIL6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_59095</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373315.1">
        <property type="nucleotide sequence ID" value="XM_007373253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIL6"/>
    <dbReference type="FunCoup" id="G3AIL6">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="GeneID" id="18872770"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59095"/>
    <dbReference type="eggNOG" id="ENOG502SFUR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110348_0_0_1"/>
    <dbReference type="InParanoid" id="G3AIL6"/>
    <dbReference type="OMA" id="YCIGTKD"/>
    <dbReference type="OrthoDB" id="1894652at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21203">
        <property type="entry name" value="ECM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442351" description="ER membrane protein complex subunit 10" evidence="1">
        <location>
            <begin position="18"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="22909" checksum="D35064CDA4D7AE92" modified="2011-11-16" version="1">MKLVNLIILSISALAAAENRINLFTKDLDTSVVEEIGYLSGKSLVLNNGELQSSASYCIGTTDVPNSECFAYVKGINSLNKATLHVFLDSNKEISHLSISHDSVADVKHRVKFHAIGTSATPNLNPQSLKKQKEQANAQTGPRVEKVTKKKIVKVVDEHGNTVDKEIEEEVEVEVDERSWVQKNWMYIVIALLMFMALGGEDKKKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIU3</accession>
    <name>G3AIU3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_135304</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Spindle pole body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373338.1">
        <property type="nucleotide sequence ID" value="XM_007373276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIU3"/>
    <dbReference type="FunCoup" id="G3AIU3">
        <property type="interactions" value="81"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIU3"/>
    <dbReference type="GeneID" id="18869880"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_135304"/>
    <dbReference type="eggNOG" id="KOG1820">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008401_1_0_1"/>
    <dbReference type="InParanoid" id="G3AIU3"/>
    <dbReference type="OMA" id="ACLNTIM"/>
    <dbReference type="OrthoDB" id="205662at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990498">
        <property type="term" value="C:mitotic spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099070">
        <property type="term" value="C:static microtubule bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061863">
        <property type="term" value="F:microtubule plus end polymerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030951">
        <property type="term" value="P:establishment or maintenance of microtubule cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990571">
        <property type="term" value="P:meiotic centromere clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046785">
        <property type="term" value="P:microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000022">
        <property type="term" value="P:mitotic spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000019">
        <property type="entry name" value="Cytoskeleton-associated protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048492">
        <property type="entry name" value="Stu2_CTS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045110">
        <property type="entry name" value="XMAP215"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048491">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12609">
        <property type="entry name" value="MICROTUBULE ASSOCIATED PROTEIN XMAP215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21042">
        <property type="entry name" value="Stu2_CTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21041">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TOG" evidence="6">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="6">
        <location>
            <begin position="273"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="515"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="759"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="615"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="518"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="759"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="817" mass="90364" checksum="7B8A657A76F5B572" modified="2011-11-16" version="1">MAEEEQDYSAIPLDERLVHKVWKARLHAYTEIVGTYETSRNEQDPCFNVSSDLFKKAIMDANVVAQEQGYNALVKYLTFGGTPANFNRIKSGLIGPICEKGLNSSRKNTKEYAQEVLLLMIEITDQPDTIIEEIIPYFGNRLPKTVAGCVSALAAIYENFGCQIVSPKPVIPCLVKLFAHSDKNVRAETMKLTVELYKWMRDALSATLLPSLKPVQQKDLTAAFEAVSGVAPEQKRLTRSQKQQLERENEQQAIGDDQDVDMEENNQPVDPLAFIDPVEVLSKFPANFEARISSSAWKERVEVLDEIVPILQRVVKLVPDDDYTAVMRLFAKCILKDANIQVVQLSANCTEMIAKGLGSNFQKYQSLVLSPLIERSKEKKPSVAQALDNALDAIFMVSGGDVGSILEAAINGMKLKTPQNKISAANFVKRCLSSTTVAPISSEIDAIMEIGIKLLSESQAPIRQAATEMIGTLMKITGERELNSFLTKVEEHRKAQIIAYFETAEVKCKLKSAPAPAVQTSSTTQRSNGGFPKKTLQASNGIGAATKLGARTSLPHSSIPAKRMATSPAKRSEEVKASNFGRGLTSRPITNSNVRSRAPGGGIAAGASVSASTDDSRYQNEIAELKNQIKDYQHQQSQQESRIKSLQTENSRLQQDLQNVVLQLENEKRNIEIASSVNETNINKVRSELKKSHDRIIQLERQLDLERIQSTKHQPYLSESTRSPLRPSTIIESNELSAGVKRLSIEGDIAMRGSMSPSFQYSGSTSSYSPITPANLPSHDTRNIEDDWKRAAEVTSQLRARIEKMKARSRNSTMNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AIX4</accession>
    <name>G3AIX4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Brix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_54072</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373369.1">
        <property type="nucleotide sequence ID" value="XM_007373307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AIX4"/>
    <dbReference type="FunCoup" id="G3AIX4">
        <property type="interactions" value="913"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AIX4"/>
    <dbReference type="GeneID" id="18871831"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54072"/>
    <dbReference type="eggNOG" id="KOG2963">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026936_2_0_1"/>
    <dbReference type="InParanoid" id="G3AIX4"/>
    <dbReference type="OMA" id="KDYTVMT"/>
    <dbReference type="OrthoDB" id="10261452at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045112">
        <property type="entry name" value="PPAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12661">
        <property type="entry name" value="PETER PAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12661:SF5">
        <property type="entry name" value="SUPPRESSOR OF SWI4 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="2">
        <location>
            <begin position="25"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="394" mass="44623" checksum="A506CF228752B939" modified="2011-11-16" version="1">MAKRRQKTRTHKKVSEEELAKIPRSMVLHLGASLKNHSLTQLVRDFRNVMQPHTAINLRERKSNRLKDFIVMAGPLGVSDLFIFNQSESTGNISLRMGKMPRGPTLQFKINSYSLVKDVRKILKHPKSVGKDSKEFLTPPLLVLNGFSSQINDVEHHEKLMITMFQNMFPPIQPQSTKVSTIKRVLMISKDPQTQEIEIRHYAINTKLVEENRNVKKLINSHHNLKKHLPNLAKNSDVSDLLLDQYSVGGMTSDSEVEDDAIVEIKQEEAANVVKKKEATAAAASVPKEETTTKKRAIKLTELGPRINMSLVKIEEGLIGSSKTLYHATITKSELEQKELAKKHELKQKLKAGEQVEENQSEEDKDMSESDSDAPEINPEDYENDSDLYSDVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ13</accession>
    <name>G3AJ13_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flavoprotein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_59958</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HFCD (homooligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374109.1">
        <property type="nucleotide sequence ID" value="XM_007374047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ13"/>
    <dbReference type="STRING" id="619300.G3AJ13"/>
    <dbReference type="GeneID" id="18873152"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59958"/>
    <dbReference type="eggNOG" id="KOG0672">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014402_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ13"/>
    <dbReference type="OMA" id="QINIITC"/>
    <dbReference type="OrthoDB" id="1532798at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990143">
        <property type="term" value="C:CoA-synthesizing protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359">
        <property type="entry name" value="HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359:SF6">
        <property type="entry name" value="PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="4">
        <location>
            <begin position="33"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="253"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="258"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="313"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="330"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW34525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="383" mass="43288" checksum="A12C4F340B5E3100" modified="2011-11-16" version="1" fragment="single">SIKKSNKTIVASPVGPPVPFQQYLTKQDDGKFHILLACTGSVATIKMPQIIDKLFQIFTTNKVSIQLVLTKSASHFLRGLKIHNDVKIWRDEDEWANFNEPSSTITPSTSTSNLNPKKPKNPYDRLILHNELRRWADIMLIAPLSANTLAKISNGIADNLLTSIVRSWSPSISGQAVKKPIIVAPAMNTFMYTHPITAKQINIITCPDFGIEVLKPVEKVLVCGDIGMGGMREWTEVVDILRRKIIAIKAEKKRQGDLLEEDENNDEDDDEEDENEDDENEDDDEEDDDDDDDEDDDDDDEEEDDDEQRNENIDNNQISTQGSSSNDKINDDDEILFEITTDDQDESQPNDNHDSLSLRPTDSVNSHHHQQKITIPKETQNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ53</accession>
    <name>G3AJ53_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pyridoxine 4-dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_59167</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373394.1">
        <property type="nucleotide sequence ID" value="XM_007373332.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ53"/>
    <dbReference type="FunCoup" id="G3AJ53">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJ53"/>
    <dbReference type="GeneID" id="18872804"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59167"/>
    <dbReference type="eggNOG" id="KOG1575">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_1_1"/>
    <dbReference type="InParanoid" id="G3AJ53"/>
    <dbReference type="OMA" id="YPVEETI"/>
    <dbReference type="OrthoDB" id="37537at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19077">
        <property type="entry name" value="AKR_AKR8A1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050791">
        <property type="entry name" value="Aldo-Keto_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625">
        <property type="entry name" value="AFLATOXIN B1 ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625:SF78">
        <property type="entry name" value="PYRIDOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="13"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="346" mass="38784" checksum="D99DC3E582538DF4" modified="2011-11-16" version="1">MSYTAVNIPGRFGFGTMSMTWKPVPPPTAQSIETLKFVTSHKEFGTKLLNGGEFYGPDDANLKLFKEFLDSNDPEANKELIVSIKGGVGPDLRPDGGRESIKRSIENIASYFPKENRPKLIFEIARVDTSIPYEETVGHIAEFVKNGTIDGISLSEVGIESIRKAITVFPISCVELELNLFSPEVINTGILKELSAHQIPLIAYSPLCRGLLTDYCVEHADTFLDEIPEGDFRLYLDKFQPDNFKHNLVALKKLYDYAHNVKKTTLETLALSWILKISGKENFKGIPKVTHILPIPSGSTTARVTSNFSNFIELTDEDLQAIDKIFEENPIRGLRYNEHLEDSLMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJ60</accession>
    <name>G3AJ60_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alcohol acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_59178</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373401.1">
        <property type="nucleotide sequence ID" value="XM_007373339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJ60"/>
    <dbReference type="FunCoup" id="G3AJ60">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="GeneID" id="18872809"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59178"/>
    <dbReference type="eggNOG" id="ENOG502QTAU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044733_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJ60"/>
    <dbReference type="OMA" id="NTIYFEM"/>
    <dbReference type="OrthoDB" id="2150604at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052058">
        <property type="entry name" value="Alcohol_O-acetyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010828">
        <property type="entry name" value="Atf2/Sli1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037:SF1">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07247">
        <property type="entry name" value="AATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW33817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="460" mass="52760" checksum="0ECF5016B8CE02C4" modified="2011-11-16" version="1">MSLVTTRSPSFLERFQICKTVYNYYSNFAIISEYSHEIDSANLSHALRNVILANPVLGYNFFRVEDGDQVHDGKNFRLSPLNEVLFSDVVSEEKFEVDSEFLEYVNTIHFELDTQKPLFKCIVNGKYIALVFNHAYVDGNSGTSFHKDLLHELNKLDEKETLDVLVTKSELNGIPKSVDELTTLFHPPLLYTLGALLKRFLVPTWLTNLWYKLSYPNLSKYPLFRHKTIQPNTAVKYRIINIPTSECKSIISYARQNETTFTPYFTGLAFVSFQNKIAPFLSDKPKSMDATIVIDGRRYYPELSNELKYQGCVTASDIIVEPVTNLGETVRYITQSLSKDIKSRERFHYVGLLKYLNIFELYTSKIGTFGRPVFEVSNLGRVNEQGEKLDIDQLWFTQDNGFSSMITFSVISTAKKGMNIVIGSSEELEDIRNPETNEKLMDEFTTDLKSSLTNYTKTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJJ3</accession>
    <name>G3AJJ3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1742-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_70142</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373480.1">
        <property type="nucleotide sequence ID" value="XM_007373418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJJ3"/>
    <dbReference type="GeneID" id="18875309"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70142"/>
    <dbReference type="eggNOG" id="ENOG502S7NV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088285_2_1_1"/>
    <dbReference type="InParanoid" id="G3AJJ3"/>
    <dbReference type="OMA" id="SGPDWFY"/>
    <dbReference type="OrthoDB" id="2158714at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013640">
        <property type="entry name" value="Vfa1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28218">
        <property type="entry name" value="VPS4-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28218:SF1">
        <property type="entry name" value="VPS4-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08432">
        <property type="entry name" value="Vfa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="65"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="21887" checksum="F5BA5448AD3B6C90" modified="2011-11-16" version="1">MNKAPPFPNKYNIRLVAANDSKSCTICYKPSTTVLVSDNQADFFYTCPQHLLDEHFATPIQPQSYLDLVKQKEELEAKKTQLEKDIELEKPYLWNKVSEYWQKSDNKKEKSKFEKLNDELKQVIEQLSDKQKEVSEFKFKVYKLNLDIYKNRLMLTQKKKYQKERYEKMHSEGFFPSAPKNDIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJJ5</accession>
    <name>G3AJJ5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DASH complex subunit DAD4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Outer kinetochore protein DAD4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_59275</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DAD4 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW33898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373482.1">
        <property type="nucleotide sequence ID" value="XM_007373420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJJ5"/>
    <dbReference type="FunCoup" id="G3AJJ5">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJJ5"/>
    <dbReference type="GeneID" id="18872846"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_59275"/>
    <dbReference type="eggNOG" id="ENOG502S890">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2591312_0_0_1"/>
    <dbReference type="InParanoid" id="G3AJJ5"/>
    <dbReference type="OrthoDB" id="5516652at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013959">
        <property type="entry name" value="DASH_Dad4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28222">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28222:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08650">
        <property type="entry name" value="DASH_Dad4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="15" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW33898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="80" mass="9645" checksum="51A0C88BFC52FD31" modified="2011-11-16" version="1">MDDPFEKEQNLLRARIISRMETLNESMATMNKNLQEINRKNLEIENVTRMWSNYMRYSDQFSKVNETPSGQTRAREQEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJU9</accession>
    <name>G3AJU9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">U3 small nucleolar RNA-associated protein 25</fullName>
            <shortName evidence="9">U3 snoRNA-associated protein 25</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_134197</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">DEAD-box RNA helicase-like protein required for pre-18S rRNA processing, specifically at sites A0, A1, and A2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Nucleus</location>
            <location evidence="2 9">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the UTP25 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373584.1">
        <property type="nucleotide sequence ID" value="XM_007373522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJU9"/>
    <dbReference type="FunCoup" id="G3AJU9">
        <property type="interactions" value="1278"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJU9"/>
    <dbReference type="GeneID" id="18869789"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134197"/>
    <dbReference type="eggNOG" id="KOG2340">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018705_0_1_1"/>
    <dbReference type="InParanoid" id="G3AJU9"/>
    <dbReference type="OMA" id="GIMIFIP"/>
    <dbReference type="OrthoDB" id="10264378at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010678">
        <property type="entry name" value="UTP25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053939">
        <property type="entry name" value="UTP25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053940">
        <property type="entry name" value="UTP25_NTPase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12933">
        <property type="entry name" value="ORF PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12933:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 25 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06862">
        <property type="entry name" value="Utp25_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22916">
        <property type="entry name" value="UTP25_NTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="6 9" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="UTP25 NTP hydrolase-like" evidence="12">
        <location>
            <begin position="248"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="UTP25 C-terminal" evidence="11">
        <location>
            <begin position="527"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="99"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW34000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="717" mass="84005" checksum="C485BE0084C6A8DA" modified="2011-11-16" version="1">MARQVKRKSHNRGDHVVDLTKRTKHGRAQMRTVTRTAARKPRDHQEPEQEEIESSEEEPEVEEEQEEEFDKGQVYNALLTLLKSDHKEHKPKKRVAEQSTEEQVEEEEEEEDNVAGINMEEEEEDADEDVDDHIDFVDEDEESPKGLNQDPYEVHFNQPLDDYMASQEKLCVKENQKWNITDKKVLNDLSLTSMLQVPPGESVSRPLSNVNSLDDYLIKKKVLDAYKREYSEDLTDLESTLLEPILNYVDVSYQYKHFKNTQYRKLYILHALNHIYKTRDRILKNTTKLHSYRESLKSKEGEVEDEEPEYRDQGFTRPKVLILLPTREACYEVVETMIQLSGTEQQENKKKFTTQFHTPAEPPNNKPDDFIDAFKGNNNDFFCIGMKFTRKSLKLYSSFYSSDIILASPIGLSMILENPDKKKRQYDFISSIEVLIIDRANQIEMQSWDHVNTVMKYVNKIPKEFHDADFSRIRMWSINDQAKLLRQTLVFSEFQTPMINNLISHKSFNLAGKLKFKPIITQENSIMNSIGLKIKQIFQRFESPSPMSDPDSRFKFFTNTILPSLLKSSSYEDGIMIFIPSYFDYLRVKSHLKANTKFNFASIDEYTSQSKLTRTRQQFTSGKIKILLYTERLHYFRRFEISGVKTLVMFGLPSNPVFYKELIRFIGKSVFKQECDLDLALVKILFSKWDAVTLERIVGNERAPVLCNSVNESYEFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJX3</accession>
    <name>G3AJX3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_70226</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RICTOR family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373608.1">
        <property type="nucleotide sequence ID" value="XM_007373546.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AJX3">
        <property type="interactions" value="484"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AJX3"/>
    <dbReference type="GeneID" id="18875312"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_70226"/>
    <dbReference type="eggNOG" id="KOG3694">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001013_1_1_1"/>
    <dbReference type="InParanoid" id="G3AJX3"/>
    <dbReference type="OMA" id="EIRIHAT"/>
    <dbReference type="OrthoDB" id="271111at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028268">
        <property type="entry name" value="Pianissimo_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028267">
        <property type="entry name" value="Pianissimo_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029453">
        <property type="entry name" value="Rictor_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029451">
        <property type="entry name" value="RICTOR_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029452">
        <property type="entry name" value="RICTOR_V"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13298">
        <property type="entry name" value="CYTOSOLIC REGULATOR PIANISSIMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13298:SF11">
        <property type="entry name" value="RAPAMYCIN-INSENSITIVE COMPANION OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14663">
        <property type="entry name" value="RasGEF_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14666">
        <property type="entry name" value="RICTOR_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14664">
        <property type="entry name" value="RICTOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14668">
        <property type="entry name" value="RICTOR_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01303">
        <property type="entry name" value="RasGEF_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01307">
        <property type="entry name" value="RICTOR_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01308">
        <property type="entry name" value="RICTOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01310">
        <property type="entry name" value="RICTOR_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR N-terminal" evidence="4">
        <location>
            <begin position="207"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR middle" evidence="3">
        <location>
            <begin position="642"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR" evidence="5">
        <location>
            <begin position="1063"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="106"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW34024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1303" mass="149150" checksum="6B43FFC14BEB8BEE" modified="2011-11-16" version="1">MVESTTNSNKASPTKNGNGSNRSQQDRPAALAAALAATASKPVKRYINPFVMAPYTANQVQHQSLDRKRSATVSSVDSIPIQSVLNRELYNPSSEMHIHTEGEEVNDYGITDSDDKTDTEDATIRPVLGGKPNSLTTPTLEPHFGFGRPPVVRKRSATTSIIENAYFPEFDYNEEDEQDNAKSPTWLLSDLLSNLGSIRDTDEYTIVAKANDLVLLFQQHPKLKNELEIKTVLPKIQFMLYHPVSEVRSSGYRILRYMISNYESLSLLIQSKLLIYLIVSLSGANSTLVEMEQALKLIREFLHIDKGTDFLSTGVIKALIAIVESGDDEKTNYGQSMIESIPESFKNVCLETICEMTIFNPELVFHSGGFKVIINSILEGSVEISSTCLIILLKLCDFQNSRKFIRNGFDLDSLISIFSNIVDDDDKVKKVSNSKLQKISFLIATLLKNFNGLIAFSIENFTSINNLVINLHKKNSKIRDFIMDIFLDCLGIQVFPWLENSAIGNSITRFNEFVGRTSYSFTYPHLKPIEHEFEQQVVNHYYGLLALVLIRNDIFDRLVDIIEQNSNESNTKKATLLLTHLYAMANSLLPPELIRPNMLLRDLSLHATFKIITRDKFQPKPDYLVNMKSTIKKINIESRYNIDDTEFKTKINNTKILTIKEFEEWNWTLLVTLIQGPLSNPKRFDEVLEKYPKFFKRLMSFYRPFKFRFSNTSLNNRNSMKYINIGTQLLEMFLNLDNGTKYLASSKLLPQLAEIVAQVDPYSGISSKEPLLNKKRLDSTCCMGYLRFIGILSAKPSGIQMLEHWQFFTLFLHIIDHSSESESNNLFIITLFKNVDFTINSRFRILLEQASKISNVKVRSYLLKHLYPSLIKIKECESFIIKILVTSNLYDSNHDIAINTVDLLYSFYSDNDFANLNTLVGLSPSISVLSQFKNGRLLLIHFMKIPIGFKYLEQSGYIDQEFSKWDDITDFQYLKKVELLIKYQLFPFLGNFEDPQINDCSLSVYFFKYLLTTEEGLGYFNHGKGRDFLELLINSITDSFEAINTNEEYFDIENQDEEHINYINKLKQNLWIIGNIAQGHYGIQLLDPLYNLNLDHSVIDIILDNFNNCPVWSLRGVCFFIIGMISSTSEGIEILDEYNWISVLDEYENSKGLAYPQSPNGIFNVEITNPYRDTRYYHIFNVGSTTTAPSLWGISNNESVDTISTGEESGEDSLNVKVNERVITLLHHLNSILASIENRAIKELNRMKQASPELFSDVGLFLEVIKLIDKGNFKLQKRIFIFGLFNMKILENLLKPERRGSFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3AJZ0</accession>
    <name>G3AJZ0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Agglutinin-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_134874</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ALS family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996500">
        <property type="protein sequence ID" value="EGW34041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373625.1">
        <property type="nucleotide sequence ID" value="XM_007373563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AJZ0"/>
    <dbReference type="FunCoup" id="G3AJZ0">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="GeneID" id="18869852"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_134874"/>
    <dbReference type="eggNOG" id="ENOG502RGCG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031316_2_0_1"/>
    <dbReference type="InParanoid" id="G3AJZ0"/>
    <dbReference type="OMA" id="IAPNCAN"/>
    <dbReference type="OrthoDB" id="3981162at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903561">
        <property type="term" value="C:extracellular vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030446">
        <property type="term" value="C:hyphal cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030445">
        <property type="term" value="C:yeast-form cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044403">
        <property type="term" value="P:biological process involved in symbiotic interaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030448">
        <property type="term" value="P:hyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1280:FF:000001">
        <property type="entry name" value="Agglutinin-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2430">
        <property type="entry name" value="Agglutinin-like protein, N-terminal domain, N2 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008966">
        <property type="entry name" value="Adhesion_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008440">
        <property type="entry name" value="Agglutinin-like_ALS_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024672">
        <property type="entry name" value="Agglutinin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043063">
        <property type="entry name" value="Agglutinin-like_N_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033504">
        <property type="entry name" value="ALS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011252">
        <property type="entry name" value="Fibrogen-bd_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793:SF2">
        <property type="entry name" value="AGGLUTININ-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793">
        <property type="entry name" value="ALPHA-AGGLUTININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05792">
        <property type="entry name" value="Candida_ALS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11766">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01056">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49401">
        <property type="entry name" value="Bacterial adhesins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0134">Cell wall</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 16" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0843">Virulence</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442450" description="Agglutinin-like protein N-terminal domain-containing protein" evidence="16">
        <location>
            <begin position="18"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Agglutinin-like protein N-terminal" evidence="17">
        <location>
            <begin position="53"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW34041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="448" mass="47941" checksum="B5E6249EB035693A" modified="2011-11-16" version="1" fragment="single">MFKFLIFNLLALTLVASEQISGIFTSFDSLVWQKAASYPFAGPAYPSWISTLSWKIDGSTMSPGDTFTLNLPCVFKFTTSQTTVNLNVGSVNYATCTFNPGDIVVAFSKLECVLLDTVTPSLDAHGTVNFPVAFNVGGSALSTDLQDSTCFHNGQNTVSYYDGDNKLSTQATFAGGSTDDPSKIVYVNRVVPSLNKQQHYLLGGNCPSGYRSGSIGMKIQNSGSQIDCSSVQMAITNSLNDWYFPENADENFSYTYSCSSTSFVVNYQNIPAGYRPFIDALVQVNNGVSISMNYVNTYTCTGSSRSTNNGKTISWGAYENNVAGGNGEAVEVVTQTYTGSTTKISTMPFSTSSDTTKTIVVNVPIPTVTTTTTYVGISTSYTTESALPGSTANVIEYNPVHTTTTVTTCSPGQVSTITTTYSTSTWATDTVFIVTECPSPSSTEEEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKE5</accession>
    <name>G3AKE5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_150267</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374417.1">
        <property type="nucleotide sequence ID" value="XM_007374355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKE5"/>
    <dbReference type="STRING" id="619300.G3AKE5"/>
    <dbReference type="GeneID" id="18870861"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_150267"/>
    <dbReference type="eggNOG" id="ENOG502R6NR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009505_1_0_1"/>
    <dbReference type="InParanoid" id="G3AKE5"/>
    <dbReference type="OMA" id="RCGYLDF"/>
    <dbReference type="OrthoDB" id="25921at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052400">
        <property type="entry name" value="Zn2-C6_fungal_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47657">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN ECM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47657:SF7">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN ECM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="26"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="866" mass="100500" checksum="B66DF2DA4422D172" modified="2011-11-16" version="1">MPVYSKSFPRASERKSRRTHKNSRDGCPNCKSKRIKCSEELPSCHNCIKKNYRCGYLDFPKDKLDMIRKKNDKKAQEIFGNKQESSHKAHVPSPFNFQDSSGNSTQTPPTNLTPLSETPNNSNQLFNDYLPMPIPKENDMDSTPPNIFNKVPQDSFDYFNVPFSKDDLRMAVYTDTMKKLMHDNNNLQLPSYTYDVFETFGDPPQRQELNHYPLHSQLNSVLDNGNFNDLDLDIDTDTDSFENSKTLFNHHFGISVNDTPKDISDNIILPFPPPETPKIGTPLPNAMIKNVSLTSLEYPKRLQQSFMKELYRNEHELLIERTNTMKSPMWTKENDEILWSTIFNKAVTFDVYFSFFMDRSLNVILKICNKSIHSGTSVTCFTSKILEQLTKKSYTYYGSLIKNLRESITQSDIEPAIISWYASWCQYLHSYATIDTLSLMYGGSTSLLCNVLNGCQTLDQIPPSLSVIMHIIESDIVACVAPDYKFDVIEELYKDLVDFKTFIIYNQELTTENNGYILKAFVEVENFVKELIEEIHPKLLYINKFYKSLYKDDDDSNDLKYISPSLLFDLLVKWLNVIPSHAISIGKNMTPLKKTFYLFFTAIGKALSNVYSVIRSVALVDALNIIHPHADFNSKTYQFDISQVSGTEQYQYLYNLSSKLIRVITFFNVRQQLITHYTEKNRETITKECHVKMVEPKHDRMQVEMGDIIHVIPEKVDIKEVMVSNFSINTIINLYNYPILNDFYTNLNDESTELKHAIEHENHDQKIRIKEFRAKYQSKGTHEPKSNPEYINNDNQLHDFDYGTGSFIFDYRIGSALEFFLKHQNQCRTPRTLKQLKDTFVHFESAIKELNKSIESDRQSRNAQTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKF4</accession>
    <name>G3AKF4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamine amidotransferase type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_150292</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374426.1">
        <property type="nucleotide sequence ID" value="XM_007374364.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKF4"/>
    <dbReference type="FunCoup" id="G3AKF4">
        <property type="interactions" value="731"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AKF4"/>
    <dbReference type="GeneID" id="18870863"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_150292"/>
    <dbReference type="eggNOG" id="KOG0573">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012368_2_0_1"/>
    <dbReference type="InParanoid" id="G3AKF4"/>
    <dbReference type="OMA" id="HAKICIL"/>
    <dbReference type="OrthoDB" id="10252281at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051857">
        <property type="entry name" value="Asn_synthetase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937:SF1">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="5">
        <location>
            <begin position="150"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Asparagine synthetase" evidence="4">
        <location>
            <begin position="468"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW32911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="630" mass="72482" checksum="C70D7DB8B3977207" modified="2011-11-16" version="1">MCGILLRTSSSLEIPSCIPSVTTQGNFKTWETQDVTEISKYLDHGSQLNPLTPGQQYKLENLDHLRQLYVQISKLNNNVKLDPDIKKKQVDEIQLEIDNRTKDDPTLPVADDINQLIYKVSTRGPNYLNFTQYGKFQMFSSILSLRQPFTKQPIIRDQFILQFNGELYNQECLELNDTQFIIDTLHENLGNDRRDAILTTLKQLSGEFAIILIDLVENRIYFGRDSIGKRSLCYNLSDSELVISSVSTIDFIECKNEFYEYDVESHAMNVHKIHSLPVHIHGPYEPEDILLGELYTNLKKSTDIRQITIHPLVHEVNESKLGVLFSGGLDCTIIAGLICELSSPTTIDLLTVGFENPRTNQSSDQTPDRKLALKSWFHLQKQYPHITIQLLEINVDYKSWLVHKSKVMDLIYPCDTEMDLSIAIAFYFASSKLPQLTRMTTLSDRNIDWTSFLENPDKYTHKTENYNSQAKVLFSGLGADELFAGYSRHEALFTNIQPDTPQAAIDECYNKLTSELIYDISIIHKRNLGRDDRVISYWGKELRYPYLDEDFINWVIQYIPPQYKFKYAFKANKKGILRMDSTRKYLLRALAVKMGLDWVSTELKRAIQFGAKSAKLEIGQSKIKGTDNVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKH6</accession>
    <name>G3AKH6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-dehydropantolactone reductase</fullName>
            <ecNumber evidence="4">1.1.1.358</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">2-dehydropantolactone reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Ketopantoyl-lactone reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60276</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(R)-pantolactone + NADP(+) = 2-dehydropantolactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16719"/>
            <dbReference type="ChEBI" id="CHEBI:18395"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.358"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>isatin + NADPH + H(+) = 3-hydroxyindolin-2-one + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:68608"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27539"/>
            <dbReference type="ChEBI" id="CHEBI:28536"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="1.1.1.358" evidence="4"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374448.1">
        <property type="nucleotide sequence ID" value="XM_007374386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKH6"/>
    <dbReference type="STRING" id="619300.G3AKH6"/>
    <dbReference type="GeneID" id="18873311"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60276"/>
    <dbReference type="eggNOG" id="KOG1577">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_0_1_1"/>
    <dbReference type="InParanoid" id="G3AKH6"/>
    <dbReference type="OMA" id="GQHIPVA"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047011">
        <property type="term" value="F:2-dehydropantolactone reductase (A-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042180">
        <property type="term" value="P:ketone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19071">
        <property type="entry name" value="AKR_AKR1-5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF13">
        <property type="entry name" value="ALDO_KETO REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="7">
        <location>
            <begin position="33"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00050878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00051098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00066965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00079693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00081322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="317" mass="36261" checksum="8526EFAB54445A13" modified="2011-11-16" version="1">MSQTVSMQLTKNSKYKLNNGQYIPVAGFGVYLMPAEKTAELVYQALVEGYRHIDSAVGYYNEREAAQGVAKFLKDHPNVHRRDIWFTTKIRNQDQGYEETKESVKQIAADVKEYIDYVDLVLIHSAKTNKEKRLGTYKALQEFVGVNPVLQIKSIGVSNYGIHHLEELFNWDGYLIKPVVNQVELHPWLPRIELREYCAKLHILLEAYTPLTRGEKFTDPELVQLSQKYKISPADILLKWSYLQGFIVLAKTENPQRIRENLDALPDGPGDAFENPLGRRNSTLGRLDLEVEIIETLNKPNSKEVLTWGGVDPTLCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKK0</accession>
    <name>G3AKK0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60940</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375120.1">
        <property type="nucleotide sequence ID" value="XM_007375058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKK0"/>
    <dbReference type="GeneID" id="18873647"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60940"/>
    <dbReference type="HOGENOM" id="CLU_1860088_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKK0"/>
    <dbReference type="OrthoDB" id="4016968at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="25"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="138" mass="16696" checksum="DE430C8ECE383341" modified="2011-11-16" version="1" fragment="single">MSLQYSQHNHVDVNGTIFPIKNDENRSILSMPYDCIFEVLQYLNQIDLVNVCLVNRELCYLARRKLYRSIFVVILGPPIEIKMNVYNYFYTNYTIMNRADYRMDHLLKNPNLQFCQLIRFNFIKPVFCQRVQRLYPRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKM1</accession>
    <name>G3AKM1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">superoxide dismutase</fullName>
            <ecNumber evidence="5">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_60963</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <topology evidence="3">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Cu-Zn superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="5"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375141.1">
        <property type="nucleotide sequence ID" value="XM_007375079.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKM1"/>
    <dbReference type="STRING" id="619300.G3AKM1"/>
    <dbReference type="GeneID" id="18873657"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60963"/>
    <dbReference type="eggNOG" id="ENOG502S5NX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063073_1_1_1"/>
    <dbReference type="InParanoid" id="G3AKM1"/>
    <dbReference type="OMA" id="ACANITQ"/>
    <dbReference type="OrthoDB" id="159229at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000007">
        <property type="entry name" value="Cell surface Cu-only superoxide dismutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6" id="KW-0134">Cell wall</keyword>
    <keyword evidence="12" id="KW-0186">Copper</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0843">Virulence</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442204" description="superoxide dismutase" evidence="16">
        <location>
            <begin position="19"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="17">
        <location>
            <begin position="45"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW33626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="211" mass="21465" checksum="6EEBB2FB31B18666" modified="2011-11-16" version="1">MVSTSSITVLALSSLALAQNAPVSTDSPQGVTYVAKFVPNSKSNISGTVQFEAAKNGSVLVNVDVQGLPSQGGPFPYHIHEKPVPSDGNCTGTLGHLNPFNGTTTSTVAAGKEAGDLAGKHGNITTSPFQTEFVDNYISLNPDNAAYIGGLSVVIHASDNSRLTCANITKEDAKKSNETETSAVTSANAAGNLVANGFVAGALAGVVAYLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>G3AKM9</accession>
    <name>G3AKM9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_50489</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375149.1">
        <property type="nucleotide sequence ID" value="XM_007375087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AKM9"/>
    <dbReference type="GeneID" id="18871446"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50489"/>
    <dbReference type="eggNOG" id="ENOG502RQK8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_821753_0_0_1"/>
    <dbReference type="InParanoid" id="G3AKM9"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="338" mass="38372" checksum="F30AD541E900B50A" modified="2011-11-16" version="1">MKKQEVPKRTVFITKLETYFSTVIPEEVTDSTSTETSVDSNTITDVESTTAAHSSMVTTLPSPYYTASETFTTTETAVASNQERPISKQELLAMEEIKRLEQLLLDLKKEMLLQQENFRLQMLSIYNATKEVTTTKQTTQTAQTTNVIGLDHEKNHGKNILNQKNPSNSVLIVPQFQNHRKENEEHSTHVYHHEYAKPKVPWYGVAATTETTTSDEISIAPTPEPEKDNLHPFAPIKTGEVQDPYDYVITYDVTESLQVSKDTQSTQSDVTETPIATEDTTTVTATATVTKRRDYRFRNNNSTIDFDVFHLDVSENQGNHYKKSFLIWLLASVLMCLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALD2</accession>
    <name>G3ALD2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CNH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_50087</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VAM6/VPS39 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374690.1">
        <property type="nucleotide sequence ID" value="XM_007374628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALD2"/>
    <dbReference type="STRING" id="619300.G3ALD2"/>
    <dbReference type="GeneID" id="18871402"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_50087"/>
    <dbReference type="eggNOG" id="KOG2063">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_290702_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALD2"/>
    <dbReference type="OMA" id="YECESYD"/>
    <dbReference type="OrthoDB" id="5325112at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032914">
        <property type="entry name" value="Vam6/VPS39/TRAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019452">
        <property type="entry name" value="VPS39/TGF_beta_rcpt-assoc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019453">
        <property type="entry name" value="VPS39/TGFA1_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894">
        <property type="entry name" value="CNH DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894:SF49">
        <property type="entry name" value="VAM6_VPS39-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10366">
        <property type="entry name" value="Vps39_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10367">
        <property type="entry name" value="zf-Vps39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CNH" evidence="6">
        <location>
            <begin position="16"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="4">
        <location>
            <begin position="688"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW33175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="971" mass="110534" checksum="0B48B0BDC84BBF2F" modified="2011-11-16" version="1">MIDIIEPCNRIRIPEGSRITSIANDHSKIYAGLSNGDVLVYTKSPTPDNKEQPGSAATTTSGKIRSLNDVKSLFSDNSESKFVSLTATFKNVSSDGTAIDNITTVKLGGEPVKSVIVVTTESSVRIFEIIGTHINQIYKVEDSKAASAVLYLEQHEQRLLYVGIKKKLVVFQISNKTRNLFSFVKLHELTMKDKIRTIDKFNGKILVGLSTDYWIINDQFEVSPLDDTFTHPTSFSYFGLSPVPRLWTINQDDTTLLVRDTQAVTIEPDLTMAVSPVKFQSVPLQIVSIYPLYLLAVYSKKIEVIDSETGDLIQRFQHYINSNQIYITGDDAQITISSGSDILQFTIAPYQQQIDQYLALSGPVTDEPKNDLKHKGLAKAISLVTKIPVHDSLFTGEKAKHMMLRKLYTSKAILLFNSYSKYHEALVEIGSEWLVSFHDILSLFPDFINGESSLFPESLPEEKEMGAIKRVKVDDLTGASINTESEYDTDTASRRSPTLKKSPVMIRRFQKAVNNLIIYLTDQRRILASFQDKPTMQWKGIDITPREIYPAPHDTKKTQLEEVATIIDTSLFLCYFHVKPMLLGPLLRLPSNRCDSKVVNQCLSRGNFGASFIKELLDFYYGRNLHDEALSMLHKLAHESTDELVQGPTLTIQYLQKLTGDHIDLVFKYAGWVLDECDEKVSECRLIFMNDSYQCESYDPEQVLNYLVDRNLGVVYLEWLLNDSDFELKGKQLTNFETKLATLYLDSLKLFTGDDENFYALEYYTKLYNFLKVRTHYDPWKTLKQIPTTQDKFLRLVIFVYKRLEEHQKSIDVLFNQLNDLEGAMDYALDIYGTTPHLGTGLFHKLLDDLLNYDDNFDKIETLLSLHGTKMQIHKVLTQLPDTFPISKLTQFLTHTFANNHKLLHNSRIKAQLYKVGTVNMEDKVLKIQSEGYKISSSKQACAVCNKRLGYAVFGVTKDNQVVHYGCLQSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALD6</accession>
    <name>G3ALD6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gag1-like clamp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60485</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374694.1">
        <property type="nucleotide sequence ID" value="XM_007374632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALD6"/>
    <dbReference type="STRING" id="619300.G3ALD6"/>
    <dbReference type="GeneID" id="18873439"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60485"/>
    <dbReference type="eggNOG" id="ENOG502S6I5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049905_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALD6"/>
    <dbReference type="OrthoDB" id="5576875at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053274">
        <property type="entry name" value="Fluconazole_resistance"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025124">
        <property type="entry name" value="Gag1-like_clamp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28065:SF1">
        <property type="entry name" value="DUF4050 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28065">
        <property type="entry name" value="FREQUENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13259">
        <property type="entry name" value="clamp_Gag1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gag1-like clamp" evidence="1">
        <location>
            <begin position="113"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="201" mass="23399" checksum="A8F9B02A26FD458A" modified="2011-11-16" version="1">MIRTNKGYNSTEEQFLKDFKKYKSLDNMAEAYSPKENPSDSAKVQTTLQDIIRQQYGDLREGSDDEDLEEDDTEYDISYHDLDLNQLRKEFEQLIVGNSDGNISSDTDTLFSQSNIGSTLWEYRRSKWLANNHPEKLEARLKSSSIDHIPKDTYVKIYNNLIDKSRQLKDSKRINLKDLIKIINAGWIAQEKWERAGRGLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALE5</accession>
    <name>G3ALE5_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial</fullName>
            <ecNumber evidence="4">2.1.1.201</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ubiquinone biosynthesis methyltransferase COQ5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">COQ5</name>
        <name evidence="6" type="ORF">SPAPADRAFT_60513</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Methyltransferase required for the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol + S-adenosyl-L-methionine = a 5-methoxy-2-methyl-3-(all-trans-polyprenyl)benzene-1,4-diol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28286"/>
            <dbReference type="Rhea" id="RHEA-COMP:10858"/>
            <dbReference type="Rhea" id="RHEA-COMP:10859"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:84166"/>
            <dbReference type="ChEBI" id="CHEBI:84167"/>
            <dbReference type="EC" id="2.1.1.201"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion inner membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. MenG/UbiE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.201" evidence="4"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374704.1">
        <property type="nucleotide sequence ID" value="XM_007374642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALE5"/>
    <dbReference type="FunCoup" id="G3ALE5">
        <property type="interactions" value="487"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALE5"/>
    <dbReference type="GeneID" id="18873444"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60513"/>
    <dbReference type="eggNOG" id="KOG1540">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_0_1_1"/>
    <dbReference type="InParanoid" id="G3ALE5"/>
    <dbReference type="OMA" id="MNDVMSM"/>
    <dbReference type="OrthoDB" id="5310959at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008425">
        <property type="term" value="F:2-methoxy-6-polyprenyl-1,4-benzoquinol methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01813">
        <property type="entry name" value="MenG_UbiE_methyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004033">
        <property type="entry name" value="UbiE/COQ5_MeTrFase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023576">
        <property type="entry name" value="UbiE/COQ5_MeTrFase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01934">
        <property type="entry name" value="MenG_MenH_UbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01209">
        <property type="entry name" value="Ubie_methyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51608">
        <property type="entry name" value="SAM_MT_UBIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01183">
        <property type="entry name" value="UBIE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01184">
        <property type="entry name" value="UBIE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 4 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="164"/>
            <end position="165"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW33189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="295" mass="32755" checksum="B3FBF4387F3754E3" modified="2011-11-16" version="1">MKTSLTRSLKRGGPILARTLATTPPPPPPPPKPETTHFGFKTVQKEDKEKLVGGVFSSVASKYDVMNDAMSMGVHRLWKSHFIDRLDAGMRPGSSRPLDFLDVAGGTGDIAFGLLDHAEKKFGDVSSTMTVADINPDMLHEGELRFQKSKWNDGSDRVKFLVQNGEIMDKIEDNSKDVYTIAFGIRNFTDVQAGLNTAYRVLRPGGIFACLEFSQVENPAIDYAYQAYSFSLLPLMGQLIANDRDSYQYLVESIRKFPNQETFKGMIQKAGFYVPEPGYENLTFGVASIHIGVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALG2</accession>
    <name>G3ALG2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_150970</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGG/PIGN/PIGO family. PIGO subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374720.1">
        <property type="nucleotide sequence ID" value="XM_007374658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALG2"/>
    <dbReference type="FunCoup" id="G3ALG2">
        <property type="interactions" value="655"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALG2"/>
    <dbReference type="GeneID" id="18870885"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_150970"/>
    <dbReference type="eggNOG" id="KOG2126">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004298_1_0_1"/>
    <dbReference type="InParanoid" id="G3ALG2"/>
    <dbReference type="OMA" id="EDEYVIM"/>
    <dbReference type="OrthoDB" id="272139at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16023">
        <property type="entry name" value="GPI_EPT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037675">
        <property type="entry name" value="PIG-O_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039524">
        <property type="entry name" value="PIGO/GPI13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23071:SF1">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23071">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="478"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="510"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="552"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="582"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="649"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="681"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="720"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="784"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="809"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="882"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="918"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="953"/>
            <end position="978"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW33205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="993" mass="112862" checksum="C0228D99F38FA7E5" modified="2011-11-16" version="1">MDIDTNPPTDGNKSSARIQQQSRTRLRQQKLRYTFIGYIIVLCFLAITQFIGVGFFTKGFLLSRHVLPNIAECVDNSCMRPAKYDKAIFLVIDALRFDFTIPVAGSTEYYHNNFPILYDLAQKENGLLLKFIADPPTTTLQRLKGLTTGSLPTFIDAGSNFNGDHIDEDNWLLQLRRANKTIAFMGDDTWTALFDDYIDPQMNFPYDSLNVWDLHTVDNGVIEHLYPLIQKENSTQWDLLVGHFLGVDHVGHRYGPRHFSMKEKLNQMNEVLEHVVSNLDDDTLLVVIGDHGMDSTGNHGGDAPDELESTLFMYSKSGKFLKKDESAYDVSNEGRNYRSVNQIDLVPTISLLLGLPIPYNNLGFPVDEAFSSHEEILTASYNTLKQLKTFRDNTPNLAHTLLEQFDTLIQHYHDKTYSEQDILLMAKEYQYLSLEQCKSLWARFDLRLIGIGLGILFLALTFMITYSRSIPAVRVSTMSFEFIGSVIAMTMLGLVLSFSIYIVLKPTDFTIWNCLAIGTALGMVIGFWAPIMDRFSINFLWNQIIDFFMYNFNSWSFMGIVFVIAHCLIFASNSYVVWEDKMVSFFLLTFGIGSIYNCAVNTELSRPARILGLSHAITFTIMTRLASMINLCREEQRPYCEATFKTTWWSVLLLHVISYILPMFIASFYKLSDSYHSAAPLWIGTGLSFLLFMNAVYWTFEYVQNSEYFNNMNLIIGIPLIKSLKLAIARLVLFITLVLANFSWSRGPLCVKLELKEENSDESDVSDEAETPGKTATILGYGNVYGSSYFLLVLNFTVAIMLTTKPIGAVSISMLIIQILSLLELSDILAIRKNLISPIIFGLLGYQHFFSTGHQATIPSIQWELGFMTTETIFMPFTHLNIVLNTFGPFIIVSLAIPLITLWRIVPSNKPITVLSQIITNVTTLITYHLFTGLSSLLFAAHFRRHLMVWKIFAPRFMLSGLLIIMLNLFLIVVTLWFGTGRILTQINRIFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALN7</accession>
    <name>G3ALN7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cation efflux protein transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_60615</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374795.1">
        <property type="nucleotide sequence ID" value="XM_007374733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALN7"/>
    <dbReference type="FunCoup" id="G3ALN7">
        <property type="interactions" value="280"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALN7"/>
    <dbReference type="GeneID" id="18873492"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60615"/>
    <dbReference type="eggNOG" id="KOG1483">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013430_4_3_1"/>
    <dbReference type="InParanoid" id="G3ALN7"/>
    <dbReference type="OMA" id="CLFHQHG"/>
    <dbReference type="OrthoDB" id="9944568at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000026">
        <property type="entry name" value="Zinc/cadmium resistance protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45820">
        <property type="entry name" value="FI23527P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45820:SF4">
        <property type="entry name" value="ZINC TRANSPORTER 63C, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="9">
        <location>
            <begin position="12"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="194"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="368"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="370"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW33280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="482" mass="53605" checksum="8F06674D2F074C62" modified="2011-11-16" version="1">MQNKEIRIWSLLGLDTVFFLLEAVIGYSVHSLALIADSFHMLNDIISLIIALWAVKVKNTKRADGKYTYGWQRAEILGALINAVFLLALCFTIIMDAIQRFFEPTEVTNPKLILVVGIAGLCSNGIGLVLFHEHGHSHGHSHSHGDEEQQVGDVVVRSPRRSSFVTETTPLIKADIPTSGSQVFDYLPDTVVERYSSPPPGHPHSPLSQVIQQESPKQQPHDHSSEQQHSHSHSHTEPETKPRKRKSLNMEGVFLHVLGDALGNIGVIITALIIWKTEYSWKHLADPVTSLLITLIIFNSALPLCRKSSKILLQATPPYINSELIVEQILRLPLVKNVHDFHVWNLNEDILIASLHVELVENCATPVQAKHTTPTPPTNNAVFSVSPTDSSVSGESDEEAIADPIDRKLFLTVVAQVRDVLHKFGIHSVTIQPEFPKLREIIHRRRESMASLTGQDHNVYGGTTKSNCLVDEYTNCDTQSCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALR0</accession>
    <name>G3ALR0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_66281</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374818.1">
        <property type="nucleotide sequence ID" value="XM_007374756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALR0"/>
    <dbReference type="FunCoup" id="G3ALR0">
        <property type="interactions" value="340"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ALR0"/>
    <dbReference type="GeneID" id="18875095"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_66281"/>
    <dbReference type="eggNOG" id="ENOG502R17J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008516_0_0_1"/>
    <dbReference type="InParanoid" id="G3ALR0"/>
    <dbReference type="OMA" id="ISHAIHI"/>
    <dbReference type="OrthoDB" id="196165at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030950">
        <property type="term" value="P:establishment or maintenance of actin cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053039">
        <property type="entry name" value="Polarity_Bud-Selection_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47775">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47775:SF1">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="293"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="207"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="270"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="396"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="524"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="209"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="401"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="435"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="459"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="524"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="575"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="597"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="636"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="664"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW33303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="726" mass="82377" checksum="2AE0D176F68692FB" modified="2011-11-16" version="1">MESESNPHTPVAITPPDGESRPETAQTTNHVSSTASLPLPDDSDDDDQDLIEHNNTLESTNKLLHDLERKLAPFSPTASVSSSQYTQTDSVVHNPNHAGLGKSTISSSVLQYIINNDDDDEEDATKRIHNNIFDPNELFNERNNKFMTKFGKGYNDDKVHQQRKLTRKNSLKVNQDRLVKKSSDASLNGHIVNDNTSVRSMEAIHVDNIDDDEDEDEDYISESNNDDDDDDYDDSDLDSRNDEDTEDFEPSTPELNIEKLIDEDIETEQGDEEENLEDLYLPPSPPRSPPREIDPNKLYGLYDFSGPDPSHCTLQRDEPVHLINDEDNYWWLIRKLTKQERIERGLQEKGEDQEVVTDEEDGKVGFVPAECLETYGERLARLNCFKNEELEKKELPLESVEGGETEEGDNSVDSLSSRQNKKCNKSVTFESLGEITDDDDDEENEEDTLRRPPGFGYFIHDKITQDEDKSDEKQSEILSDIFPETPLNLPKHKRSSDKKPDKLNIADDAFEELFIQPKRASLNTLDEISIGSYSPDTPSKKEEPEEDDNSSLTRSIILDRLRKVTSDIQEQMDQTDTRSKEHIPEVEPRKEPIYSENPYQRTARQYSGTSPEYTQPDSDNNTSILEEPHDGGDEQLSPNLQSLPTPENSSSHLSLHKSKKESIDATTSSSLEDLIITPSTSVNSSSNDIKNERRKSKPVHEMFMPILGKFDELAEKLAELDDILNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>G3ALX1</accession>
    <name>G3ALX1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Serine/threonine-protein kinase BUR1</fullName>
            <ecNumber evidence="4">2.7.11.22</ecNumber>
            <ecNumber evidence="3">2.7.11.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Serine/threonine-protein kinase bur1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">SPAPADRAFT_136900</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="4"/>
    <dbReference type="EC" id="2.7.11.23" evidence="3"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374839.1">
        <property type="nucleotide sequence ID" value="XM_007374777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ALX1"/>
    <dbReference type="STRING" id="619300.G3ALX1"/>
    <dbReference type="GeneID" id="18870004"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_136900"/>
    <dbReference type="eggNOG" id="KOG0600">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="InParanoid" id="G3ALX1"/>
    <dbReference type="OMA" id="EPSHEFQ"/>
    <dbReference type="OrthoDB" id="28397at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070691">
        <property type="term" value="C:P-TEFb complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070693">
        <property type="term" value="C:P-TEFb-cap methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000415">
        <property type="entry name" value="CMGC/CDK/CRK7 protein kinase, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF233">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="23"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="19">
        <location>
            <position position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00073250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EGW33324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="343" mass="38425" checksum="B08C06842A3F46C5" modified="2011-11-16" version="1" fragment="single">MPPPPPPPQSKGHIKEMGRLKEYEVIRKLGQGTFGVVQKAKSKKTGDLVALKQLINHSAKEGFPVTAMREITILKRLDHKNVVKIVEMIYEEPKVNNPADLITQRGAFYTVSPYMSSDLVGLLENPNVELELNQIKCFMKQLLEGIQYIHEQGFLHRDIKAANILIDFNGVLKIADFGLARTYHGKIPKLGQGPGGGERAYTGLVVTRWYRPPELLLGERKYTTAVDLWGIGCVFAELFTHKPILVGKSDAHQAQLVFDLVGPPLNWPEAAKLPNKTDFNIGLTCKRSLESRFEKILPPTGVELLSGLLTLDPYKRFNALDALEHDFFKTDPLPAKPEDLPKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM02</accession>
    <name>G3AM02_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA-splicing endonuclease subunit Sen34</fullName>
            <ecNumber evidence="4">4.6.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_60693</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the tRNA-intron endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.16" evidence="4"/>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374870.1">
        <property type="nucleotide sequence ID" value="XM_007374808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM02"/>
    <dbReference type="FunCoup" id="G3AM02">
        <property type="interactions" value="150"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM02"/>
    <dbReference type="GeneID" id="18873526"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60693"/>
    <dbReference type="eggNOG" id="KOG4133">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049366_1_0_1"/>
    <dbReference type="InParanoid" id="G3AM02"/>
    <dbReference type="OMA" id="RTFSLEW"/>
    <dbReference type="OrthoDB" id="48041at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000214">
        <property type="term" value="C:tRNA-intron endonuclease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000379">
        <property type="term" value="P:tRNA-type intron splice site recognition and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22363">
        <property type="entry name" value="tRNA-intron_lyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016690">
        <property type="entry name" value="TSEN34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059049">
        <property type="entry name" value="TSEN34_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13070:SF0">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13070">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26577">
        <property type="entry name" value="TSEN34_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017250">
        <property type="entry name" value="tRNA_splic_SEN34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TSEN34 N-terminal" evidence="7">
        <location>
            <begin position="18"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA intron endonuclease catalytic" evidence="6">
        <location>
            <begin position="163"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR017250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR017250-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF26577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW33355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="267" mass="30277" checksum="14735F8A1C6DF114" modified="2011-11-16" version="1">MSENELAASKPIVLPLIDSANPEVLIFNTTAIRRLRDEYGILGVLIGTLAQYPQQNVFLSVPLKLMVWEVIWLFEQDVCVLVDWLKYCNLKKDAMNGDYKNKNLISLDKKKADTKPDFLITTNDYGEEDDWELIDQCKVTLQSYIDKYLSSPNCKTTIPQLISNYHKFKHLKNEGYFISPGLKFGGDLVIYPGDPLRYHSYSIIKFDFVDINEIIVGGRLATGVKKNMIIMESSHAKEQSSVGLDVANELFSDPTITAYSIEWAGFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM09</accession>
    <name>G3AM09_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acyl-CoA thioesterase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_60700</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374877.1">
        <property type="nucleotide sequence ID" value="XM_007374815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM09"/>
    <dbReference type="GeneID" id="18873532"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60700"/>
    <dbReference type="eggNOG" id="KOG3016">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032690_2_1_1"/>
    <dbReference type="InParanoid" id="G3AM09"/>
    <dbReference type="OMA" id="WGIDNEQ"/>
    <dbReference type="OrthoDB" id="68328at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03444">
        <property type="entry name" value="Thioesterase_II_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049450">
        <property type="entry name" value="ACOT8-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003703">
        <property type="entry name" value="Acyl_CoA_thio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066:SF34">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20789">
        <property type="entry name" value="4HBT_3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA thioesterase-like C-terminal" evidence="2">
        <location>
            <begin position="137"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW33362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="243" mass="28350" checksum="40A8BB3CEEE4FB17" modified="2011-11-16" version="1">MVYIANISLTKRNSHKEAKQKYDDYYAKIEQKAKESDADEDEEDDDDVDEEPAPPKPFVFQTPYHDWLKKYHVEELPVSNHEANLLIYHKILPEFTSLNLTPEEEQVSVTDRRLSFLIKWGIDNEQGFNQPLINVDAQFQFVGLANLSDSMYLDTLLRVLRIKDLDLTKHHEYFSVSLDHSIYFHDDDFDVTTWMGFSYKVVRFAHNRVVIEGEMYNDRGIHIATIVQEGLVMFNGAEKGAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AM67</accession>
    <name>G3AM67_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial import inner membrane translocase subunit TIM23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_54791</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion inner membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Tim17/Tim22/Tim23 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374287.1">
        <property type="nucleotide sequence ID" value="XM_007374225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AM67"/>
    <dbReference type="FunCoup" id="G3AM67">
        <property type="interactions" value="585"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AM67"/>
    <dbReference type="GeneID" id="18871888"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_54791"/>
    <dbReference type="eggNOG" id="KOG3324">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063935_1_0_1"/>
    <dbReference type="InParanoid" id="G3AM67"/>
    <dbReference type="OMA" id="QYIMPEG"/>
    <dbReference type="OrthoDB" id="159299at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005681">
        <property type="entry name" value="Tim23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045238">
        <property type="entry name" value="Tim23-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00983">
        <property type="entry name" value="3a0801s02tim23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15371:SF0">
        <property type="entry name" value="SD19278P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15371">
        <property type="entry name" value="TIM23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW32772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="218" mass="23006" checksum="D6083F0B52052941" modified="2011-11-16" version="1">MSWLFGKSSSSQTDDASLKQTLGFDPKEVTDVHAMIQSPNLYTASQLHPLAGLEKGVEYLDLEDEKLNAVEGSQGIIPSRSWSDDLCYGTGAVYLLGLGLGGAYGLQEGVKNIPANAPPKLKLNTVLNHITKRGPFLGNSAGVLALTYNLIDSSIDAIRDKHDDYNSVAAGALAGALFKSSAGVKPMFYASALMALGAGAWCGIKRAFNSNTEPQALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMA8</accession>
    <name>G3AMA8_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Altered inheritance of mitochondria protein 36, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60765</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374921.1">
        <property type="nucleotide sequence ID" value="XM_007374859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMA8"/>
    <dbReference type="FunCoup" id="G3AMA8">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMA8"/>
    <dbReference type="GeneID" id="18873555"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60765"/>
    <dbReference type="eggNOG" id="ENOG502SAUM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_997550_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMA8"/>
    <dbReference type="OMA" id="INNVVGY"/>
    <dbReference type="OrthoDB" id="4081130at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW33406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="273" mass="31602" checksum="556704CF81732D72" modified="2011-11-16" version="1">MFSTRLMPRVGLMRSPLVSRSPIGSLRNYAIQHSNIKRKRPAVRYMVGMFLFSTIFLFFVSGRVDKKKRVQTSFSEKEFDEYEEITGLKRRHKLITHEMNDKYQFYVIPYVKHEESVERLSQRLAQLDDSKNVKVIDPTELLEQEKADESKKYSYLLQDLDAANRPYPPGLITAVIKQHLTFLLNTREGTLDTTYIIKNYPKTTHEAIKFENEVSDIKKCIVLQNDVANELPKVSNDEEIRAINNVGGYFNSVGKSTTVANDKQLKSITLEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMD7</accession>
    <name>G3AMD7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Delta(14)-sterol reductase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">C-14 sterol reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Sterol C14-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">SPAPADRAFT_60137</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP(+) = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17813"/>
            <dbReference type="ChEBI" id="CHEBI:18364"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.70"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:18563"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="15">Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 2/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the ERG4/ERG24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374303.1">
        <property type="nucleotide sequence ID" value="XM_007374241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMD7"/>
    <dbReference type="FunCoup" id="G3AMD7">
        <property type="interactions" value="500"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AMD7"/>
    <dbReference type="GeneID" id="18873234"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60137"/>
    <dbReference type="eggNOG" id="KOG1435">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015631_0_3_1"/>
    <dbReference type="InParanoid" id="G3AMD7"/>
    <dbReference type="OMA" id="EWCELRP"/>
    <dbReference type="OrthoDB" id="10262235at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050613">
        <property type="term" value="F:Delta14-sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000009">
        <property type="entry name" value="C-14 sterol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001171">
        <property type="entry name" value="ERG24_DHCR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018083">
        <property type="entry name" value="Sterol_reductase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257:SF52">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE TM7SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01222">
        <property type="entry name" value="ERG4_ERG24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01018">
        <property type="entry name" value="STEROL_REDUCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="8 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="13 16" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="9 16" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="12 16" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="148"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU369120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EGW32788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="440" mass="50112" checksum="FE4F1765B8B29C0D" modified="2011-11-16" version="1">MSLNPPTTHREFSGLPGAIGITLGLPTLITFFALITNQTYSIQGINLDINAVLNQLPNSTEGWIALCFDKQCWLAYLAWFGILVALDFILPGTAAQGVELRDGSKLNYKINGPAVSSVFVAVLLARAWQVKGYFLPELQFVYDHQLELTLVTTIFSMLLAVFVYVCSFIPLTKPNGKGTHERILSVNGNTRNPIYDWFIGRELNPRIGTWDIKLFCELRPGMLLWLLINLSCVHQQYHQLGKVTDSLILVNLLQAFYIFDGVLNENGLLTMIDVTTDGFGFMLSFGDLAWVPWAYSLQSRYLSCPGNEIELGWTNLSLIVLVKVVGFYIFRSANKQKSDFRSGKLDHLKSIQTKTGSKLLIDGWWGLSQHINYFGDWLIGWSWCLPTGFQTPLTYFYVIYFGTLLLHRQIRDETKCRGKYGKDWEKYEKLVPYKIIPYIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMH7</accession>
    <name>G3AMH7_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_60173</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW32830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374345.1">
        <property type="nucleotide sequence ID" value="XM_007374283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMH7"/>
    <dbReference type="STRING" id="619300.G3AMH7"/>
    <dbReference type="GeneID" id="18873259"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60173"/>
    <dbReference type="eggNOG" id="KOG4409">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017361_2_2_1"/>
    <dbReference type="InParanoid" id="G3AMH7"/>
    <dbReference type="OMA" id="FKNHADE"/>
    <dbReference type="OrthoDB" id="121051at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035965">
        <property type="term" value="P:cardiolipin acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF23">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE ICT1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="18"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="322" mass="37469" checksum="FB99320DFA2B0E0B" modified="2011-11-16" version="1">MAALGYFVNNISEIISTPGMRLHLIDLPGFGNSSRPKFPAEFLIEPDTREAKIAQIKLVEGWFIDCIETWRIKRDLHKFNMIAHSMGAYLTSCYLMKYNRDPKLVVDRVILVSPMGTESSEVSLLSNKPTEEENKPDPENEVELLVESLEMGRPRFPKNYIIRTLWKHNKSPFMILQKLGPFYSKVLSYWSFKRFKNHADEEVIMKLHRYSYSIFNQFPGSGELAITKFINHEILARLPLCERGLIEYFKEYNVQALWLYGDKDWMNHRGGEHIHTQVSKTDPSLSQFMIVENAGHHIYLDNPAKFNSIVTKFFDLNIDSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3AMJ4</accession>
    <name>G3AMJ4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_60793</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996501">
        <property type="protein sequence ID" value="EGW33437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007374952.1">
        <property type="nucleotide sequence ID" value="XM_007374890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AMJ4"/>
    <dbReference type="STRING" id="619300.G3AMJ4"/>
    <dbReference type="GeneID" id="18873573"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_60793"/>
    <dbReference type="eggNOG" id="KOG2382">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1192384_0_0_1"/>
    <dbReference type="InParanoid" id="G3AMJ4"/>
    <dbReference type="OMA" id="FICTRTR"/>
    <dbReference type="OrthoDB" id="8119704at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118:SF4">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="76"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW33437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="233" mass="26981" checksum="40354F26A1CD1016" modified="2011-11-16" version="1" fragment="single">MIATRRFYNSVHLSRFICTRTRLYTTAANTIEAQFPPRQLLIKVEEIPDLPFVEHITLPFKHFKPAASPPDYTKTPVLFIHGLFGWKNNYRSVGQLISERTKHPVYALDLRNHGDAPHVLPHTYSAMAEDVQEFLHDRGWEKVILVGHSMGAKVAMIVSLLYPELVEKLVVIDNTPHYQPLGEQYQRDLLAMCIIESQRIGFKEVDGKRIIEKKKIDEVLSHYEPNHTVREFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANM9</accession>
    <name>G3ANM9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_67158</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375834.1">
        <property type="nucleotide sequence ID" value="XM_007375772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANM9"/>
    <dbReference type="FunCoup" id="G3ANM9">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANM9"/>
    <dbReference type="GeneID" id="18875145"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_67158"/>
    <dbReference type="eggNOG" id="KOG2049">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008419_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANM9"/>
    <dbReference type="OMA" id="YWFQNCK"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07920">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033712">
        <property type="entry name" value="Pumilio_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF80">
        <property type="entry name" value="SUPPRESSOR PROTEIN MPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="4">
        <location>
            <begin position="193"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="217"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="260"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="296"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="333"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="369"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="405"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="489"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="91"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="825"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="848"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="876"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="891"/>
            <end position="900"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW32558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="926" mass="103876" checksum="1129854C741781BE" modified="2011-11-16" version="1">MSHNHNISINSITSIIEPTTPPNLSSHQHTPTTSSCSTSSGNFQAQKLFSKPGTKTNIWDTSNKLNHNLNLPSFAIDNEVLSTPLIMPSGGGAGGGAAKAGSISSATTTTSSSQTSLRKLSVDTNNVSQSAFETGHSDMVAAGGNNNLVDSFNVAYGVSNVGDNKENMISKLTISSDDATPSSMVSTPPSCSTPSIKIPQKIDKEYLASINKLPLIQLKSEILKLSKDQYGCRFLQKKIDENLIPNYQVRHANFEIIFKQIYPYMYDLIIDPFGNYLIQKLIIYCNEANLNLLLEILQENLFQISISQHGTRALQKIIDNLNNSYQLELLIKGLKPYIIDLIKDLNGNHVIQKILNKYQPQDCQFIYDSIIDDLYIVATHKHGCCVLQKCLNHVTPAQLQQFSAAILRFDNFKLLINDQFGNYVLQYLISINSFEMNYKIFENFVQFNIGNLCNLKFSSNVVEKFLKNCFNNETMDMSFSNLKFELIYNILINDLNKLINDPYGNYVIQTLIDILINPHVDNNQVEKLSLVLPDKQQQQSHELEQNSLQIMIIKYWFQNCKIVSSFGKRIQSKINTILNNNSSTTTSSSSPATNFLFSHQPNMLNNNNKKSQMNANGEFIVNDSFYLPKQQSLGFRSSSSPIGIIGTNNEQSPAYFNGHSLQKVNNMPQSHQNRNFSLPTNLYANIPTNVKNNGNINNGTNNNCNANNVSPPIQGSTFRPPYHHESASNTPIGMNPQSGFAQNDYMVNEYSYAGHHHNHAQQQQQQPIMSLQHQSQHMVKQPIPLDTHYNANQPSYYPNHKMTMSDYSNIPNASQAQSVPHYTTTNINYGNYTPPLPQGMTGPPDLHQYYSQPQMSQQQLSQGNQGSFLPPPPPQQQQQQQQQQQLHQQQYYNNLQNTVVNGPVHGHSPSWSSGNGPLFGSITPKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>G3ANU3</accession>
    <name>G3ANU3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rpr2-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_55584</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375305.1">
        <property type="nucleotide sequence ID" value="XM_007375243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ANU3"/>
    <dbReference type="FunCoup" id="G3ANU3">
        <property type="interactions" value="81"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ANU3"/>
    <dbReference type="GeneID" id="18871952"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55584"/>
    <dbReference type="eggNOG" id="ENOG502S5IC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122019_0_0_1"/>
    <dbReference type="InParanoid" id="G3ANU3"/>
    <dbReference type="OMA" id="KCIQVNC"/>
    <dbReference type="OrthoDB" id="74314at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007175">
        <property type="entry name" value="Rpr2/Snm1/Rpp21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742:SF3">
        <property type="entry name" value="RIBONUCLEASE MRP PROTEIN SUBUNIT SNM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04032">
        <property type="entry name" value="Rpr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="20962" checksum="2646334CDD8B72F6" modified="2011-11-16" version="1">MSQEKPLNPNQITSLHLYDLAHSNAISPLSQNYSVLFERHNLIKQINLPPLLTYKFCQGCGNLWIPGLTVSIRIAYPKKKKGENVGKRRLRYRCTVCRHTSYDDSLIQEKNKKVEEPVGEKVEFKAEWTPDHTNQVKQPSVKNVKSKDRAKKRKLNLSSLLSEKKKQKTETKSLNLMDFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP12</accession>
    <name>G3AP12_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_152410</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375318.1">
        <property type="nucleotide sequence ID" value="XM_007375256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP12"/>
    <dbReference type="STRING" id="619300.G3AP12"/>
    <dbReference type="GeneID" id="18870928"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_152410"/>
    <dbReference type="eggNOG" id="ENOG502SEK2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044093_0_0_1"/>
    <dbReference type="InParanoid" id="G3AP12"/>
    <dbReference type="OMA" id="DSINQHY"/>
    <dbReference type="OrthoDB" id="4024111at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="168"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW32042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="444" mass="49195" checksum="95F7CC50BBCA2BA5" modified="2011-11-16" version="1">MEMDMNIDMNINTNIDTVSMNTPTKKIRELTLASPPPDYLNSPTSSIATCMVNKGYSHKLTNNVLSELNDRANEISMMINSPPESRSSEGSSGSSSSGNRSKRFSIIHRSRFNQMESISQHYSVHDSSSNKRRRTLNGKDEIVAIPILTNKEDEDQRKDSIRKISPVKMSSTKISPSKSPNKISPSKISPSKRSMNLHAQLNEKSIQVSDSGSPSKREFIRPTGVPKTRIGSLELSGVKSLQRKSSIPQLQDTHTLSKKPSIPQLQKKPSIPQLQKKPSIPQLQKKPSIPQLQKKPSIPQLQKKPSIPNLNKKPSTSQLYHSPSLASRVSSTTTASTTTTVTATTTTNLMPPPPPPPPPHSYTHSPSHKTISRKSSIPQLQKIPNLSRISPSKSLNHNVLHNSTNIPKSRSVTIPEPFSLYDKPTISSSQKSLNKFQRFKEKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AP49</accession>
    <name>G3AP49_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lysophospholipase</fullName>
            <ecNumber evidence="6">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_55616</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the lysophospholipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="6"/>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375356.1">
        <property type="nucleotide sequence ID" value="XM_007375294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AP49"/>
    <dbReference type="FunCoup" id="G3AP49">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AP49"/>
    <dbReference type="GeneID" id="18871954"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_55616"/>
    <dbReference type="eggNOG" id="KOG1325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014602_1_0_1"/>
    <dbReference type="InParanoid" id="G3AP49"/>
    <dbReference type="OMA" id="FIANCRN"/>
    <dbReference type="OrthoDB" id="4084751at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF56">
        <property type="entry name" value="MEIOTIC PHOSPHOLIPASE SPO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4 5 6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005131589" description="Lysophospholipase" evidence="6">
        <location>
            <begin position="21"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="PLA2c" evidence="7">
        <location>
            <begin position="55"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW32080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="736" mass="83421" checksum="1A948D2158F55869" modified="2011-11-16" version="1">MIILLLILSLVLCSNNYSYSYNTRSVVLDNSRLLYKNFSQSVQTWNVSYAPYTILCPSTSLIRPADDTLCEQERTYMENRNIQTEVHLKNLLIHNKIPQFDVEEFFTGKDIPITITIAISGGGYRSMLTGAGVLAALDERSKEKCQISGLLQATSYIGGISGGSWLVMSNFINDFKVIEEIRKEGWNLQESLLSGVPNFEPSGIQRHIEEKARERNSINKELAKQQPGIIGTFLKFFNFRSNSTIPVSEKVQENSILYTWIKGLFTRRTTPSIVNTPTTNPSLVNHTNEMALKEYLKFYKELLIEVKDKKRAGFHTSLTDYWGRALARKIFTTAARTPGVTMTAATNILSSFQNYEQPFPIIGTVEKDPTGGDSTSVDSHSFEFTPFEFGSWDSYLNAFIPMKYLGTTLKSGISTQPSPIENQSYCISGFDNVGFITATSSSLFNHIFVYVYQMLNNFQIEATIALENILKTFGLSSQWKSLRSPQLHPDYALYSPNPFYLYGPNTTISDSPDLYLVDGGDDGQNIPFQPFLRPARKVDIILTYDMSCELHNFPNGTVLTNTANRFLHGKYQLPRFTLQQNGNSVAKSIFPKVPTPQEILRYGLSTRPVFLGCDLMRDYETVPTTSNNVSNITTKEILLDYFPPVIVYTANSNYSYFGANSSTFQLSYTDYEVDSMINNGFNMASYLNSSLYPVCLNCIILKRQFDRRQYNKENTYIPKTCLGCYEAFCWDRARFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>G3APU2</accession>
    <name>G3APU2_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61345</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375539.1">
        <property type="nucleotide sequence ID" value="XM_007375477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APU2"/>
    <dbReference type="STRING" id="619300.G3APU2"/>
    <dbReference type="GeneID" id="18873852"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61345"/>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030658_0_0_1"/>
    <dbReference type="InParanoid" id="G3APU2"/>
    <dbReference type="OMA" id="YDVSYQE"/>
    <dbReference type="OrthoDB" id="1517790at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW32263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="436" mass="49943" checksum="F49D8E7EFAAF378A" modified="2011-11-16" version="1">MEKNNPLLPFAFTSSVINDINPLNTTPRARTVIDENASSRYHTRTTHLVTPESDRRVPLGDRIVPSLKRKLTSFNERDDIKRADIELPTEIDDHEELPPSSPPVEIPLMSEFDFSTTTHAVPESPKHIHNLPSEPDVILPHRNISSDADFGIDRFNRFKVSFNDLPSSNADDEYKDTGFNKARSVILEAFESIETVINLQSMNLHDLPNEIKDLNNLVIFDTASPSYELYLSHNKLTTLPPSLFKFTKLNVLVLRHNKISRIPPLIEKLTNLTDLTLSTNKISWLPYQLLHLPKLQTFSAGPNPLMRIPEDDAIECNYTLSTHECHHRTSINYLSPHKSKVPSLKSLCLDKIANYDITYKETRIWKQQTPKVFHTLMAKAIAQGKQRDTCNQCSLIVVEPVAEVFEWWDILENRNIPIRKQFCCQGCVDRYLNSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>G3APV0</accession>
    <name>G3APV0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxymuconolactone decarboxylase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_61352</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996502">
        <property type="protein sequence ID" value="EGW32271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007375547.1">
        <property type="nucleotide sequence ID" value="XM_007375485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3APV0"/>
    <dbReference type="FunCoup" id="G3APV0">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="GeneID" id="18873854"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61352"/>
    <dbReference type="eggNOG" id="ENOG502RCP9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065389_3_0_1"/>
    <dbReference type="InParanoid" id="G3APV0"/>
    <dbReference type="OMA" id="PKSINAM"/>
    <dbReference type="OrthoDB" id="5537330at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052999">
        <property type="entry name" value="PTS1_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28180">
        <property type="entry name" value="CONSERVED MITOCHONDRIAL PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28180:SF2">
        <property type="entry name" value="PEROXISOMAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW32271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="283" mass="31864" checksum="1EC055FB6ADDDD6F" modified="2011-11-16" version="1">MLTAERLAELASYEHIQDSWYIPAIACISQLNLPEDAPVICYFALQRRVLNAVDLVKLAQQCIDTVRRAQQDPSYEVPQLVSEDLPLLCSRNDVLGMQQTLIDQTREVILKISVMIGMPKSINAMMALKAATPKCLMATVHRTTMLESDTSEVTPNSINVDQLSLEINRGTHFWNAVYSKISQRVQTQIYNAYPDLWNYIFHHIYSPLLSYTDILPGKETSFAAIACMIPQDVNPQLKGHLKGALNNGASVEQVNSVRQLVLDMCEWKGDVTWKDGKESIPRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ37</accession>
    <name>G3AQ37_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_154455</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376162.1">
        <property type="nucleotide sequence ID" value="XM_007376100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ37"/>
    <dbReference type="STRING" id="619300.G3AQ37"/>
    <dbReference type="GeneID" id="18870985"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_154455"/>
    <dbReference type="eggNOG" id="ENOG502RPU7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_473257_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQ37"/>
    <dbReference type="OMA" id="YLECDNS"/>
    <dbReference type="OrthoDB" id="4026135at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="583" mass="67313" checksum="EF79717CDEC0C81C" modified="2011-11-16" version="1">MKNNLLDQPPPGSIHFDDLPMEIINQIIYWISDTDLDLNLDPAIPTHRSKNNSSPKYVYVLNHVRCTGYYSTLVSLSSTNSFLRQKLGPILFKNISLIRINSIDSVLCNPKRSEFYSDKKKYQREFIKELLETNFQACTTEELARTSFKSHILGDATYKSRFELKFAMSNYVTYLECDNSVLRGSTLSLFPNLKALKVLDEASEDPLVGNYQIENLDYLAINARTLMNCAKMLKLLPRLKRLDLLLDYCEIPIHLGLTKIIDEFRNADKTNLTELALNLNSSFSIAYEDTITLLDVISNNSELTILSLRTKRRKYFDYPRQDQSRWPLYRSYSGDKFLLFLKSLDHLIIDIPILEVLRFHPHTYNRCPDASVEWDKMRTKTLTLVDQASIGPQFSTYSRDGAGVLFQAAQFTELNFQYGEALEEGHLHALKLVSDFVQFMVDCGGSDNRCRGYAGLKNLAIEKCWSVTDDSIRREYYCNIMLKLNALEDVTNQSTKYKLASAAVWSRTPFNSPRYRIRDVYGITYKEHRNSFDGAIPARSHRYEAVFHPKADSANNNVFWSIETSLADFEQYTVRQRKSSLWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQ77</accession>
    <name>G3AQ77_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">SPAPADRAFT_61991</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376202.1">
        <property type="nucleotide sequence ID" value="XM_007376140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQ77"/>
    <dbReference type="FunCoup" id="G3AQ77">
        <property type="interactions" value="132"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQ77"/>
    <dbReference type="GeneID" id="18874138"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61991"/>
    <dbReference type="eggNOG" id="KOG3601">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064525_2_0_1"/>
    <dbReference type="InParanoid" id="G3AQ77"/>
    <dbReference type="OMA" id="EWWKGRN"/>
    <dbReference type="OrthoDB" id="6250593at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050670">
        <property type="entry name" value="STAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45929">
        <property type="entry name" value="JAK PATHWAY SIGNAL TRANSDUCTION ADAPTOR MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45929:SF7">
        <property type="entry name" value="LAS SEVENTEEN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="95"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="157"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="168"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EGW31424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="270" mass="29553" checksum="E70BA3A3C7E49284" modified="2011-11-16" version="1">MSTALINRSLTTIRTELEFLRDSEVITEGLYDKLVSALPDKFQKDSKAWDVDRLTNGTSTPAVDNTSALADSLAKTSIASPPAYAPPTNAKPASKPVSYCTAIYEYRAQESDDISLSAGDKIAVVEHLSEDWWKGYKKGSEKTGVFPSNYVRSISESEYNGGQSEKSQYQVVPQQQQQQPPRQEPSYGGYAQFPPPSTNYYPPQQYNPPPPQQYSPAPVQQQPQQVVVEQPQQSHHNGGDMAKKFGKKLGNAAIFGAGASIGSNIVNSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQF6</accession>
    <name>G3AQF6_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_62080</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376281.1">
        <property type="nucleotide sequence ID" value="XM_007376219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQF6"/>
    <dbReference type="GeneID" id="18874180"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62080"/>
    <dbReference type="HOGENOM" id="CLU_3070176_0_0_1"/>
    <dbReference type="InParanoid" id="G3AQF6"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW31503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="53" mass="6384" checksum="6073707A244C4319" modified="2011-11-16" version="1">MRRLKPEIKVTSDFKSPFEEIGSLDNNRQLNLEDRLIYVRKVTNHAITKFYCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQN0</accession>
    <name>G3AQN0_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peroxin-7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">SPAPADRAFT_62194</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the WD repeat peroxin-7 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376355.1">
        <property type="nucleotide sequence ID" value="XM_007376293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQN0"/>
    <dbReference type="FunCoup" id="G3AQN0">
        <property type="interactions" value="479"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQN0"/>
    <dbReference type="GeneID" id="18874212"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62194"/>
    <dbReference type="eggNOG" id="KOG0277">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046581_1_0_1"/>
    <dbReference type="InParanoid" id="G3AQN0"/>
    <dbReference type="OMA" id="FAVHWNL"/>
    <dbReference type="OrthoDB" id="273771at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062137">
        <property type="term" value="C:cargo receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005053">
        <property type="term" value="F:peroxisome matrix targeting signal-2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044536">
        <property type="entry name" value="PEX7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46027">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46027:SF1">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="100"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="237"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="278"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EGW31577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="370" mass="41833" checksum="E49DB0CAB4510995" modified="2011-11-16" version="1">MLSFRTQGYNGYGVQYSPYFDNKIAVATAANYGLVGNGRLFILNINPQGIIGEQISWETQDGLFDLAWSEIHENQVVAASGDGSIKLFDITVGQFPIMNWKEHQREVFSVRWNLVDKANFVSSSWDGTMKIWSPQRQESLLTLSAPTSDNKVAPVGTAKPQLSHQPQHQQQQINTANCIYSASFSPHSPSQIISCNGSSQIQMWDIRAPKPLQLQYVAHRGLETLSCDWNKYKSTVIASAGTDKSVRIWDLRMISKLDQPYSPISNTHIRGPTPLNELLGHQFAVRRVQWSPHDSKELVSTSYDMTARIWRDESDERARFLNTKQGGMTGVFGHHKEFVIGCDYSLWGEPGWVATTGWDEMVYVWDSKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQQ6</accession>
    <name>G3AQQ6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Agglutinin-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">SPAPADRAFT_155003</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376381.1">
        <property type="nucleotide sequence ID" value="XM_007376319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQQ6"/>
    <dbReference type="FunCoup" id="G3AQQ6">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQQ6"/>
    <dbReference type="GeneID" id="18871005"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_155003"/>
    <dbReference type="eggNOG" id="ENOG502RGCG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031316_1_0_1"/>
    <dbReference type="InParanoid" id="G3AQQ6"/>
    <dbReference type="OrthoDB" id="3981162at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2430">
        <property type="entry name" value="Agglutinin-like protein, N-terminal domain, N2 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008966">
        <property type="entry name" value="Adhesion_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024672">
        <property type="entry name" value="Agglutinin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043063">
        <property type="entry name" value="Agglutinin-like_N_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033504">
        <property type="entry name" value="ALS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011252">
        <property type="entry name" value="Fibrogen-bd_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793:SF2">
        <property type="entry name" value="AGGLUTININ-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793">
        <property type="entry name" value="ALPHA-AGGLUTININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11766">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01056">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49401">
        <property type="entry name" value="Bacterial adhesins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012858885" description="Agglutinin-like protein N-terminal domain-containing protein" evidence="14">
        <location>
            <begin position="16"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Agglutinin-like protein N-terminal" evidence="15">
        <location>
            <begin position="50"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="339"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EGW31603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="365" mass="40741" checksum="6BDBCF9839BBF433" modified="2011-11-16" version="1" fragment="single">MLFLLLLLALQTALGATKYVNAFTSFDDLKFMTPKRPSQPFQVYWEATLSWSLKSSEIQPYDTFGLILPCVFKFATNENTLTLKVNEVELAFCSLISGELKETFSKLDCIALPGVTLGNSKGGIKLPFTFNVGGSALSTDLETSKCFTAGKNIISFYDGDKELSYPAFFETMFESGGKHDADEVVYGARSLENMKWLQNYLLAGNCPQGYTSGKLGFTILQGAKIDCNTIHAYISNKFNDWFFPTTFEEMKMTINCSENEVLIEYQNISPGYRPYLDGLISTRLGHDTKMKYVNQYTCNNETVKSNDVIVNWANYQPIYPTNTGGTLVVQELISSTTTSDGLSTIDSETYENEDPIPTVATNNEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>G3AQT9</accession>
    <name>G3AQT9_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_62248</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376414.1">
        <property type="nucleotide sequence ID" value="XM_007376352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AQT9"/>
    <dbReference type="FunCoup" id="G3AQT9">
        <property type="interactions" value="705"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AQT9"/>
    <dbReference type="GeneID" id="18874230"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62248"/>
    <dbReference type="eggNOG" id="KOG0756">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_5_1_1"/>
    <dbReference type="InParanoid" id="G3AQT9"/>
    <dbReference type="OMA" id="AWYAGCT"/>
    <dbReference type="OrthoDB" id="10003116at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071913">
        <property type="term" value="F:citrate secondary active transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006843">
        <property type="term" value="P:mitochondrial citrate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000007">
        <property type="entry name" value="Mitochondrial tricarboxylate transport protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049563">
        <property type="entry name" value="TXTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788">
        <property type="entry name" value="SUCCINATE/FUMARATE MITOCHONDRIAL TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788:SF4">
        <property type="entry name" value="TRICARBOXYLATE TRANSPORT PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 13" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="8"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="106"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="206"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW31636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="294" mass="31247" checksum="E7126E63FD1B4641" modified="2011-11-16" version="1">MSKSDKKVDPFKSFIAGGTAGAVEGVITYPFEFAKTRLQLVSKSAITSRNPLVLIYTIAKTQGVSALYVGCPAFVVGNTVKASVRFLGFDSIKALLADKNGKLSGPRGVIAGLGAGLLESVIAVTPFEAIKTALIDDKQTAKPKYQNGLVSGTVKLVKDMGFKGIYAGVVPVSLRQASNQAVRLGSYNAIKTMIQQAQGQKPTDPLNSGATFAVGSFAGIITVYTTMPIDTVKTRMQALGADKLYKSTIDCFVKIFKQEGLLTFWKGATPRLGRLVLSGGIVFTIYEKMLVLMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AR56</accession>
    <name>G3AR56_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine/threonine-protein phosphatase 2A activator</fullName>
            <ecNumber evidence="6">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Phosphotyrosyl phosphatase activator</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_141506</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PTPA-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="6"/>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376009.1">
        <property type="nucleotide sequence ID" value="XM_007375947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AR56"/>
    <dbReference type="FunCoup" id="G3AR56">
        <property type="interactions" value="550"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AR56"/>
    <dbReference type="GeneID" id="18870380"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_141506"/>
    <dbReference type="eggNOG" id="KOG2867">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030733_0_0_1"/>
    <dbReference type="InParanoid" id="G3AR56"/>
    <dbReference type="OMA" id="YLWGAAQ"/>
    <dbReference type="OrthoDB" id="16120at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008160">
        <property type="term" value="F:protein tyrosine phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006970">
        <property type="term" value="P:response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04087">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1150:FF:000002">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004327">
        <property type="entry name" value="Phstyr_phstse_ac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043170">
        <property type="entry name" value="PTPA_C_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037218">
        <property type="entry name" value="PTPA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012:SF5">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A ACTIVATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03095">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016325">
        <property type="entry name" value="Phstyr_phstse_ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140984">
        <property type="entry name" value="PTPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0697">Rotamase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="358" mass="41093" checksum="383A31E67DAA434B" modified="2011-11-16" version="1">MATPVKRITTRDDLDKWLDSQTFNQVLSFITDLQESIVGKPNDEPITESQIVTQLQAVLDSINTIIDNHPVIQEKDISRFGKVEFRDFYDNVAKQSPSLLSPLLTNQEHTLEIATYFNESWGDRTRIDYGSGHELNFICVLFCLKQLDCITPQDYPGLLLHVFTKYMKTMRRLQKQYWLEPAGSHGVWGLDDYHFLPFLFGAAQLSTHPHMKPKSIHNPELVEMYKNKYMYMECIDFINNIKTTRNGEKLSIRWHSPMLDDISAAKSWAKIKEGMVKMYKVEVLGKLPIMQHFMFGQLIPCPPGVSAPHEHHEDECGHIHSKDLVNTWGDCCGIKIPSAIAASEALKKEQSQKPIPFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARJ5</accession>
    <name>G3ARJ5_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_61887</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376094.1">
        <property type="nucleotide sequence ID" value="XM_007376032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARJ5"/>
    <dbReference type="STRING" id="619300.G3ARJ5"/>
    <dbReference type="GeneID" id="18874096"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61887"/>
    <dbReference type="eggNOG" id="ENOG502QQ2N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007860_1_0_1"/>
    <dbReference type="InParanoid" id="G3ARJ5"/>
    <dbReference type="OrthoDB" id="2427554at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016287">
        <property type="term" value="F:glycerone-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052744">
        <property type="entry name" value="GPAT/DAPAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31605">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31605:SF0">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="443"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW31316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="496" mass="56647" checksum="2ED34CCB9DFBE8B0" modified="2011-11-16" version="1">MALGAMEHNPNCNVKIVPCGMNYFNAHKFRSRAVVEFGDPIEISHDLVKKYTNPETNREAVRELLDIITSGLKAVTVTCPDYETLMLIQAGRRLYAGNFAQQLPLPLIVEMNRRLVMGYEHYKNEPKVQQLKEKVLAYNDMLKSFQLPDHHVEKLEDSNKLHLIPILITRVIKLLILFVLALPGATLFSPVFLSTKIISKRKAKEALANSVVKIKANDVIATWKILVSMGIAPIVYSFYASIGTYYCSKHGYFTSFKLFWVWVILYISGIFVTYSALLTGEQGLDLFKSIRPLYLSISTSSITELKTMRHELSEEITELVNEFGPQLFPNDFNLLELKDHLKISDDVNYVDSDEEEEIKTQQLRLRRLTRRKQERKKKKNGLDAFNNNSSDISDGLLTSDNSLSNIPMFSDYQLYRNSYLDLQDNSTSTSSIYDDYRYAQADQNSPAQHAVHAKSPLSRPGLDNSSSSEQIELNFAGKSLSDKIKNKMREKREEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARK3</accession>
    <name>G3ARK3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_61891</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376102.1">
        <property type="nucleotide sequence ID" value="XM_007376040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARK3"/>
    <dbReference type="STRING" id="619300.G3ARK3"/>
    <dbReference type="GeneID" id="18874099"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_61891"/>
    <dbReference type="eggNOG" id="KOG2177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063947_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARK3"/>
    <dbReference type="OMA" id="DANDNWH"/>
    <dbReference type="OrthoDB" id="6105938at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006301">
        <property type="term" value="P:DNA damage tolerance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039577">
        <property type="entry name" value="Rad18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14134">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RAD18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14134:SF3">
        <property type="entry name" value="RING-CH-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="32"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="276"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="292"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="314"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="340"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW31324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="369" mass="41487" checksum="01B87482D1371E0E" modified="2011-11-16" version="1">MSHKLEDSKVWNELDHSLTSHLLAKINNIMECPICSEVMIIPVTAECGHSFCYGCIHQWFETKLNCPTCRTDIEHKPVLNIHLKEISKGVVDLLIETTTDEKEKSHLVKLRDESTKNYETDKTGKQLFGNLFKGTTMLIDNSDGVPRCGNCHWEAHGNVCMHCGTRFRNARNLVDGDLDGDDEDEDRFEDVNSDNDYDLDDSFIDGRNIGEIFRDVIEDYDSELDLDYESGEPYQLDANDNWHGFESASETGSFELSGVDEDDLDSSVIDALDEFHHGDSDSNIEGSLGDGSDSHRGFYSDREDDHNGNSGYGGDDDDDGNNNFDDDVEHHFDGGNHGFDDDDANEGYDENESYGEDSYGGDSGYEDNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARN3</accession>
    <name>G3ARN3_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor SLU7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_51763</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SLU7 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376564.1">
        <property type="nucleotide sequence ID" value="XM_007376502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARN3"/>
    <dbReference type="STRING" id="619300.G3ARN3"/>
    <dbReference type="GeneID" id="18871592"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51763"/>
    <dbReference type="eggNOG" id="KOG2560">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019317_3_0_1"/>
    <dbReference type="InParanoid" id="G3ARN3"/>
    <dbReference type="OMA" id="INEESWA"/>
    <dbReference type="OrthoDB" id="249612at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030628">
        <property type="term" value="F:pre-mRNA 3'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021715">
        <property type="entry name" value="Slu7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039974">
        <property type="entry name" value="Splicing_factor_SLU7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12942:SF2">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SLU7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12942">
        <property type="entry name" value="STEP II SPLICING FACTOR SLU7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11708">
        <property type="entry name" value="Slu7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor SLU7" evidence="9">
        <location>
            <begin position="62"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="160"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="193" mass="22624" checksum="9BE9EEE4D8D49112" modified="2011-11-16" version="1">MSQPNQYIPKYITSKPWYQGNKDDDSTDYLSHHRVNNEIVDHSIPTTGHGIKDSDVRIQHEDYDSKRDRWYGYTSDEWLAHLDKWTEKKEEEQKSDEPDYELELIELDLLENQLVNTKQHPLEKLIRDRNDIPGYIHNITSNPNNKIKLEFDPKSRLVKDPSAGFINDSNQFVKKLTGEGQPNANDDENEARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>G3ARN6</accession>
    <name>G3ARN6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Defective in cullin neddylation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_51766</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity.</text>
    </comment>
    <dbReference type="EMBL" id="GL996503">
        <property type="protein sequence ID" value="EGW31789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376567.1">
        <property type="nucleotide sequence ID" value="XM_007376505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ARN6"/>
    <dbReference type="FunCoup" id="G3ARN6">
        <property type="interactions" value="423"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ARN6"/>
    <dbReference type="GeneID" id="18871593"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_51766"/>
    <dbReference type="eggNOG" id="KOG3077">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047042_0_0_1"/>
    <dbReference type="InParanoid" id="G3ARN6"/>
    <dbReference type="OMA" id="LWCKFLQ"/>
    <dbReference type="OrthoDB" id="27198at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032182">
        <property type="term" value="F:ubiquitin-like protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14351">
        <property type="entry name" value="UBA_Ubx1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.200">
        <property type="entry name" value="Cullin, PONY binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014764">
        <property type="entry name" value="DCN-prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042460">
        <property type="entry name" value="DCN1-like_PONY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005176">
        <property type="entry name" value="PONY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281:SF31">
        <property type="entry name" value="DCN1-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281">
        <property type="entry name" value="RP42 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03556">
        <property type="entry name" value="Cullin_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51229">
        <property type="entry name" value="DCUN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DCUN1" evidence="3">
        <location>
            <begin position="59"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU410713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW31789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="283" mass="33181" checksum="ED0CA45CFB1ED64F" modified="2011-11-16" version="1">MSKQQVKRQFQELTGVPGPQAQQFVENANYNLNAALDNFYNRNASPATTTTTNRKPPVKSDKRLVSLFKKYREDDEHIGIDGTLAYLEDLSITPEDPLALTLAYFLKSPRVGVFNKDAFLTIWQHYECYTITQMKNVILHVHDDILDSGNQYIDVMEDKPLTLKGMYEFTFEFLKEVENQRVLDVQTCIDYWKLLLPLVLKKVGAPVKQQVEERLDQWYEFVLEDHKKPFSFDGWCQFYLFVQDIIIKDPVAFKDYDEMQSWPNVMDEYIEYLRENGLVEGVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS50</accession>
    <name>G3AS50_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nucleoporin NUP188</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_142824</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Nup188 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377042.1">
        <property type="nucleotide sequence ID" value="XM_007376980.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="G3AS50">
        <property type="interactions" value="157"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AS50"/>
    <dbReference type="GeneID" id="18870488"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142824"/>
    <dbReference type="eggNOG" id="ENOG502QQFV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001029_1_0_1"/>
    <dbReference type="InParanoid" id="G3AS50"/>
    <dbReference type="OMA" id="HSWKFFA"/>
    <dbReference type="OrthoDB" id="102511at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018864">
        <property type="entry name" value="Nucleoporin_Nup188_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044840">
        <property type="entry name" value="Nup188"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041634">
        <property type="entry name" value="Nup188_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048883">
        <property type="entry name" value="Nup188_N-subdom_III"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431">
        <property type="entry name" value="NUCLEOPORIN NUP188 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18378">
        <property type="entry name" value="Nup188_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10487">
        <property type="entry name" value="Nup188_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21093">
        <property type="entry name" value="Nup188_N-subdom_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup188 N-terminal" evidence="11">
        <location>
            <begin position="58"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin Nup188 N-terminal subdomain III" evidence="13">
        <location>
            <begin position="553"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="domain" description="Nuclear pore protein Nup188 C-terminal" evidence="12">
        <location>
            <begin position="1276"/>
            <end position="1609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF18378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF21093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW31009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1637" mass="186016" checksum="53B358CB7D190A2F" modified="2011-11-16" version="1">MSVVQLNSGKPGLGDPTSTELGKGDLPLKSIEPSQYWTFDNALNLLKTCHDPYILRTLDEFLLLNSKVLINPSPFANTGTASISGKEMSLRAISYDILPVNISDAETIGKYLPFLDLKEIIRVISQTCKKIPPKFCHEWTVKSKLHEDRDKLLDNERLYLYTSKLLRERRSVLKMVIELVNNKLNEFTSTTVKNLGKEIVLSKDYIGELIDSLQATCTFITERKYVTGISKKLDELVYNESILFAIQLCQVMVEVLMGQSTLVFKEVKHWFQFMKSNNFGMSLGPFITYPDSFNQLQSLFTVVSVLVLDLDGAFDGQHKYISVADVFHTINDAITNNSNNTNAVVLYSWSIIVLRKYYYIQEYPSKEFTVHELDQIINSLNPRSTQVFSQLRKHNQILHFDKIYSAILSTVIVAALPLVNLTTEVVEAIHDIASNCPNHIIERFFENESTRNTIILARTKFPLLLTPYIKLCSIHGNFALHEFNELLSYIQVFNKDEFVNMYQIDDENTELVKLTEWVDIYPPFEASNKKLSMVLKSGTKAKILPASNPNEVLVTFLYKYNGWAFLGRVLQNISKLFNSADPTKVELVVDILNLINRVVLDNGIDEAKLVLESMSAYTDDSDILEVILRLLEQGLHSRNVDILVSVIELLTNVMPFLSYRIWPYLSKSVLFTQNGKEGFAATIFGAIEMVNGDYEFTIALIKMTESLVQNCLSLDQDYPEKSKSVILSKIVTHLINLFESFIYCRFNKSYQKMEMGVLILDILSTILATVYGIDPDTPVEHKATKVFASSAVRILDAFLVTDKDSSRAASPILTMIESLSQNLEMYELNDSSGFWHETWIRCCLSYSHLLITIRSTLSYPPSAFERNLFMHLPSLVSSYSRFESIRKDILDLLTSLTNAKWDQDSATPSLLSHLGSDFSQMLLHSLAADLDNSFDDYKLKVSLYDFICAVMMGGNQEGLSVLFISGRDVFGDYTKDHEHKEVEEKLSLLSILKGNINDMKYYPNSVSIHLVDALALAFNLWTTTKENDNDVEFINTLISRVKLQMIDPPDTAESYISRCYELKLVAKVAEILALHLFTTRNDKLKQNILDFITSDFIDIAKTKFTVSDYKSNLHNNLQISFEAAFPKLKLSQFTTGLTKRNRYGISTVYNLPVMDSLFKHESEWPQIREQVIASSINLQYVNAQVSSAKSFGALITSYCRTYEPALDVRMLEFIDYLLRQNINEGIISELVSSIYYERVELGFYLMYSFYSRCPKTTSGKDTKAVFEIIKSGSTLLSSSSMNFLSSLAESQGYYRPLLKIIYCGLLLISNDETIVMEYFSLFRDLFELIITKGTKTLLIEVQNDVYLSRTDKNHKSVKMNDRIDDLMMILSILKIFIKLSGGSRLHQEMASLVVDNGTIKSLLNLYSISHAIEVNDDFIFAQLSLMYTLELMSIEVIANEFIKSGLFVVLLESPISIPIRAGGLSIAHGAQYYRLWINGILPVIITSLSKLGPGIIPEVCVALQLYNKQLENCIESWSRDSSSIRISTSIVAETNQILLIYDLLKSMNVSEYLKGKYEVSSDEIDMKILPGLESEAKRESFGECIENLLKHPKFLTSRVAPSSLEEQRIIEVGGERYEKFVKGIIDEIRDLKDYFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>G3AS84</accession>
    <name>G3AS84_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Receptor L-domain domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">SPAPADRAFT_52225</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SPS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377076.1">
        <property type="nucleotide sequence ID" value="XM_007377014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AS84"/>
    <dbReference type="STRING" id="619300.G3AS84"/>
    <dbReference type="GeneID" id="18871646"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_52225"/>
    <dbReference type="eggNOG" id="ENOG502QUZC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035846_0_0_1"/>
    <dbReference type="InParanoid" id="G3AS84"/>
    <dbReference type="OMA" id="GHNYECT"/>
    <dbReference type="OrthoDB" id="536881at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.20.20">
        <property type="entry name" value="Receptor L-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051648">
        <property type="entry name" value="CWI-Assembly_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036941">
        <property type="entry name" value="Rcpt_L-dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31018:SF3">
        <property type="entry name" value="RECEPTOR PROTEIN-TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31018">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003442583" description="Receptor L-domain domain-containing protein" evidence="9">
        <location>
            <begin position="20"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="352"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="352"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EGW31043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="415" mass="45761" checksum="174C466FC2D2943A" modified="2011-11-16" version="1">MQLKPFLLLLAYAIVDVFAADDTKTTTTSKSDPCSFSTTIIEPTGIQELNTCATLKGEITISGNAIGPIDLPSVRELAGSLTIVNSPSVVSVSLNSLQNITGKFQIKNATQLNSIDVISLERVKDLQFISLPSLSNFNFNPSIKAVEKIVLSDTALYDLSGLTHFTEINYMNLNNNKNITEIEFPNLKTVTDNLILSFNHDDAKVKMDKLIWAANLTIQDVAKFSAVNLTAVNGTLGFSYNAIEELKLDKLESVGESLQIFANDDLDNLSLKKLSEIGGEFKMFNNTELDNMTSSFPELKRVRGALSITGDFKDLQMPKLDRVQGDFKMTSKNEDFSCKHFDDLKDRGRIEGHNYECTPGKKEKLGKQGDDDDDDDDDRDSGSKKTSSKKNGGNNVLVPMNFLTSVFAFLIANFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>G3ASI1</accession>
    <name>G3ASI1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTP cyclohydrolase 1</fullName>
            <ecNumber evidence="3">3.5.4.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GTP cyclohydrolase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">SPAPADRAFT_63025</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">GTP cyclohydrolase 1 is the first enzyme in the biosynthetic pathway leading to folic acid.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP cyclohydrolase I family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="3"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW31099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377132.1">
        <property type="nucleotide sequence ID" value="XM_007377070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ASI1"/>
    <dbReference type="FunCoup" id="G3ASI1">
        <property type="interactions" value="379"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ASI1"/>
    <dbReference type="GeneID" id="18874558"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63025"/>
    <dbReference type="eggNOG" id="KOG2698">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049768_1_4_1"/>
    <dbReference type="InParanoid" id="G3ASI1"/>
    <dbReference type="OMA" id="CEHMCMS"/>
    <dbReference type="OrthoDB" id="4966at2759"/>
    <dbReference type="UniPathway" id="UPA00848">
        <property type="reaction ID" value="UER00151"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00642">
        <property type="entry name" value="GTP_cyclohydro1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.286.10:FF:000003">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1130.10:FF:000012">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00223">
        <property type="entry name" value="FolE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043134">
        <property type="entry name" value="GTP-CH-I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001474">
        <property type="entry name" value="GTP_CycHdrlase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018234">
        <property type="entry name" value="GTP_CycHdrlase_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00063">
        <property type="entry name" value="folE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006825">
        <property type="entry name" value="PRK09347.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006826">
        <property type="entry name" value="PRK09347.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109:SF7">
        <property type="entry name" value="GTP CYCLOHYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
        <property type="entry name" value="GTP CYCLOHYDROLASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00859">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00860">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="8" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GTP cyclohydrolase I" evidence="13">
        <location>
            <begin position="100"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EGW31099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="278" mass="31304" checksum="A0DD3F5B6FB4F343" modified="2011-11-16" version="1">MAEKHTLESSSEVNGSKNPHSPLPVIPTKRSKLVSGATPSHYNPNVQSLQSGTPLRQRAASPYTLNPPIDSDGLSWPSKGARSRLDESPEESKARVGRIADAVKVILEELGEDVDREGLLETPERYAKAMLYFTKGYEDNIRDVIKVFEEDHDEMVIVRDIEIFSLCEHHLVPFFGKVHIAYIPNKRVLGLSKLARLAEMYARRFQVQERLTKQIAMALLEFLRPRGVAVVIEATHMCMVSRGVQKSGSSTTTSCMLGCFRDHQKTREEFLTLLSRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT69</accession>
    <name>G3AT69_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_56792</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376865.1">
        <property type="nucleotide sequence ID" value="XM_007376803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT69"/>
    <dbReference type="FunCoup" id="G3AT69">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AT69"/>
    <dbReference type="GeneID" id="18872069"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_56792"/>
    <dbReference type="eggNOG" id="KOG0769">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_6_3_1"/>
    <dbReference type="InParanoid" id="G3AT69"/>
    <dbReference type="OMA" id="PLEMINT"/>
    <dbReference type="OrthoDB" id="446044at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015217">
        <property type="term" value="F:ADP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015867">
        <property type="term" value="P:ATP transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052217">
        <property type="entry name" value="Mito/Peroxisomal_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939:SF1">
        <property type="entry name" value="MITOCHONDRIAL THIAMINE PYROPHOSPHATE CARRIER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PMP34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="3"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="136"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="226"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW30832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="346" mass="39225" checksum="4F257EEA25BE2613" modified="2011-11-16" version="1">MQLTPIEKAASGALASAIANTLVYPLDLSKVLIQTQVKKRHPKPQATDITSSSSESDDMEDSVFKQTLTQDNELKYKNTIDVLRQIYAKKGILGWYHGLFSTVAGTAAQNFSYFYWYSIVKKVYANLYKHIPNHKPSTLMELFLGAVAAAISQLFTMPIGVITTQQQTDKHHKNLIQLVREILDQDGVTGLWRGLRVSMVLCINPSITYGSYERLKQVLYGTKEFLNPLESFSLGVLAKSMATLATQPLIVSKAMIQKKSKPKKHHNDEKHHEDEDDEEDIKFDHFTDALAHLWHTEKFHGLYKGIAPQLLKGVFVQGLLFMFKDQIDMLFLFLLSLLKGKPKARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT83</accession>
    <name>G3AT83_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">40S ribosomal protein S22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">SPAPADRAFT_58042</name>
        <name evidence="7" type="ORF">SPAPADRAFT_62706</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907</strain>
            <strain evidence="9">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the small ribosomal subunit (SSU). Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 32 different proteins and 1 molecule of RNA (18S). The 60S subunit contains 45 different proteins and 3 molecules of RNA (25S, 5.8S and 5S).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372330.1">
        <property type="nucleotide sequence ID" value="XM_007372268.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376874.1">
        <property type="nucleotide sequence ID" value="XM_007376812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT83"/>
    <dbReference type="FunCoup" id="G3AT83">
        <property type="interactions" value="1144"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AT83"/>
    <dbReference type="GeneID" id="18872297"/>
    <dbReference type="GeneID" id="18874441"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58042"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62706"/>
    <dbReference type="eggNOG" id="KOG1754">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098428_1_1_1"/>
    <dbReference type="OMA" id="LPAKNFG"/>
    <dbReference type="OrthoDB" id="10250260at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.30:FF:000001">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_A">
        <property type="entry name" value="Ribosomal_uS8_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047863">
        <property type="entry name" value="Ribosomal_uS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003115">
        <property type="entry name" value="PRK04034.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00063823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EGW34918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="14742" checksum="BA7A613CC8BC7450" modified="2011-11-16" version="1">MTRTSVLADALNAINNAEKTGKRQVLIRPSSKVIIKFLTVMQKHGYIGEFEYIDDHRSGKIVVQLNGRLNKCGVISPRFNVKINDIERWTDNLLPARQFGYVILTTSAGIMDHEEARRKHVSGKILGFVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G3AT86</accession>
    <name>G3AT86_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">soluble epoxide hydrolase</fullName>
            <ecNumber evidence="6">3.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Epoxide hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">SPAPADRAFT_62752</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Aminopeptidase that preferentially cleaves di- and tripeptides. Also has low epoxide hydrolase activity (in vitro). Can hydrolyze the epoxide leukotriene LTA(4) but it forms preferentially 5,6-dihydroxy-7,9,11,14-eicosatetraenoic acid rather than the cytokine leukotriene B(4) as the product compared to the homologous mammalian enzyme (in vitro).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an epoxide + H2O = an ethanediol</text>
            <dbReference type="Rhea" id="RHEA:19037"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32955"/>
            <dbReference type="ChEBI" id="CHEBI:140594"/>
            <dbReference type="EC" id="3.3.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="16">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.3.2.10" evidence="6"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376882.1">
        <property type="nucleotide sequence ID" value="XM_007376820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AT86"/>
    <dbReference type="STRING" id="619300.G3AT86"/>
    <dbReference type="MEROPS" id="M01.034"/>
    <dbReference type="GeneID" id="18874448"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62752"/>
    <dbReference type="eggNOG" id="KOG1047">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014505_4_1_1"/>
    <dbReference type="InParanoid" id="G3AT86"/>
    <dbReference type="OMA" id="CTALQFI"/>
    <dbReference type="OrthoDB" id="79562at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09599">
        <property type="entry name" value="M1_LTA4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000009">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.320:FF:000001">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2010.30:FF:000001">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.320">
        <property type="entry name" value="Peptidase M1, leukotriene A4 hydrolase/aminopeptidase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049980">
        <property type="entry name" value="LTA4H_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038502">
        <property type="entry name" value="M1_LTA-4_hydro/amino_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034015">
        <property type="entry name" value="M1_LTA4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015211">
        <property type="entry name" value="Peptidase_M1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726:SF3">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09127">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01263">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 16" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M1 leukotriene A4 hydrolase/aminopeptidase C-terminal" evidence="17">
        <location>
            <begin position="305"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR634015-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR634015-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EGW30849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="460" mass="53474" checksum="4668F88EEE328267" modified="2011-11-16" version="1">MSGRPVSAKDNLYTFTQPVPIPSYLISIASGDLTSAPIGPRSDVYSEPCNIKQCQWEFEHDMENFIQIAENLIFEYEWLRFDSLILPASFPYGGMEIPNLCQLTPTLICKDRSQVSVVAHELAHSWSGNLVTNCSWEHFWLNEGWTVYLERRILEGIAIAKAREKGYPNPEKYGESIRQFNAIIGWTDLENDLKSMGDNVDKYSTLVQDLKGGEDPDDAFSTVPYEKGFNLLFHIEQKVGGKKVFDAFIPYYFKTFRYKSLDTYQFREVLYNYFSDKTKELDSIDWKAWLYAPGMPPIDPKFDTTIADKCYDLAKKWYSFTQKNELPTSEFSEQDIESFDANQSVVFLDALISYTKIEGFHWKNYKDLLSIMESKYTSYSKTLNAEVLFKWFYLQVSGEVETFKVKLGEWLGTIGRMKYVRPGFMLLNQVDRELAVKYFKQFESRYHPICRAMVKKDLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATA3</accession>
    <name>G3ATA3_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_62765</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376899.1">
        <property type="nucleotide sequence ID" value="XM_007376837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATA3"/>
    <dbReference type="STRING" id="619300.G3ATA3"/>
    <dbReference type="GeneID" id="18874457"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_62765"/>
    <dbReference type="eggNOG" id="ENOG502RQ04">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1695249_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATA3"/>
    <dbReference type="OMA" id="GGIGQRQ"/>
    <dbReference type="OrthoDB" id="4003242at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="164" mass="18964" checksum="2C20A4D42D08DD91" modified="2011-11-16" version="1">MYRVKPLSRASRSISQTFKRFNSAHHHHPEPESKPFEITITKIFGIAALAGGYLVYNRDFDEPLFQHEVFDKTRSGERDDLRSENYLQRYKNAFISEYGRDKGGIGNRLYRRAGDYVVREQTLIPSQSPFAQEFGAGIKLNELGPRRERFPRFAPKDTTSTSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3ATG1</accession>
    <name>G3ATG1_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Transketolase</fullName>
            <ecNumber evidence="4 15">2.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">TKT1</name>
        <name evidence="17" type="ORF">SPAPADRAFT_142718</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 15">
            <text>D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = aldehydo-D-ribose 5-phosphate + D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:10508"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="15">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <cofactor evidence="15">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="15">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="15">Binds 1 Mg(2+) ion per subunit. Can also utilize other divalent metal cations, such as Ca(2+), Mn(2+) and Co(2+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 1 Mg(2+) ion per subunit. Can also utilize other divalent metal cations, such as Ca(2+), Mn(2+) and Co(2+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="12">Binds 1 thiamine pyrophosphate per subunit. During the reaction, the substrate forms a covalent intermediate with the cofactor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 15">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the transketolase family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.1" evidence="4 15"/>
    <dbReference type="EMBL" id="GL996504">
        <property type="protein sequence ID" value="EGW30924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007376957.1">
        <property type="nucleotide sequence ID" value="XM_007376895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ATG1"/>
    <dbReference type="FunCoup" id="G3ATG1">
        <property type="interactions" value="802"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ATG1"/>
    <dbReference type="GeneID" id="18870476"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_142718"/>
    <dbReference type="eggNOG" id="KOG0523">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_0_0_1"/>
    <dbReference type="InParanoid" id="G3ATG1"/>
    <dbReference type="OMA" id="ADYMRGS"/>
    <dbReference type="OrthoDB" id="10267175at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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</entry>
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                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
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                <person name="Jin M."/>
                <person name="Gunawan C."/>
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                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
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                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
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                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
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            </authorList>
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        </location>
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        </location>
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            <end position="323"/>
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            <end position="208"/>
        </location>
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        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
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        <location>
            <begin position="285"/>
            <end position="300"/>
        </location>
    </feature>
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            <position position="345"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EGW30968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="345" mass="37595" checksum="DC114144621E4A5B" modified="2011-11-16" version="1" fragment="single">MNEYGGLGIVIEENEEKTYAPDLNLKRPNILSNSNSMNSMMTQSTSVASSPMIIEENEHTEDQQIPVVEYAEQQDTNRNESTTTISRASIETESLLPPLPDAIEDLTTSEKPPASTTTTTTTFEDSHSTHSNDSILSNKDAEYKKTVYLTNNNPAGNFSNYALDSLNQNSSLQLFPRLSKSTLDESLSLNGSLSATNTNKSSPQSTNGTTITANDSTNSNNSTSSHSPLKKLRSIKNGIRKLSLSNMNGSSNNSSIASSPSTPKIPNLSYTQPEPIHIHTHSHSHSNSTSSNSSYSSNSVRSKRSRVPSQSSPPIMSPVITLSENLSSTKRTLNNMEQNYFDSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
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    </organism>
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            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <end position="61"/>
        </location>
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    </feature>
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            <end position="130"/>
        </location>
    </feature>
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            <begin position="151"/>
            <end position="177"/>
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    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="24970" checksum="9E802FA85D88A66E" modified="2011-11-16" version="1">MVHNVLFDPPIPEGHHINLGNDTIVDTLATIYNPRLFRNLFRVYFTFLLVQYILIYACVPLEDIENRPAICVITCMYTLVMFVINAALLPLCSSTIPTLVSLRCSFVSFVMELIGLVLHITSVVQCLNAGEMFQIERERGHTSTIKTRVDTYIIVFHVLAMCFAALATASVFCLFVICSRALEFINGKVDRRVIKRNKIYEGINSSIEAFVEGQKDYYLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007377290.1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Put_GTT3"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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            <end position="181"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EGW30381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="339" mass="38573" checksum="BFDC7BD0EA972C37" modified="2011-11-16" version="1">MSSFTTYKKADLVTVLKKLDIHTVSKDTKKVLVDKLTVYAEKSANGAATVQKLLQELADAREEGEGEGDEDVTAVEEEVEVIETEDDDEEGDEEEDEEEGEEDDQEDKDYNAPPPLNFRAVFDPVLQYYEQFVDRVYECTDKIGVTYTDYSAQLRQQLSSTVTLNYLELILEFAVYLAYYVPLVPLNKNALIPQVVLDNVPFLATSTLPSAQVTTLFAYDPLITLVFWLLTSIAFPLVLSYFFNFTRRIFEFEEDTVLFRVYAFDPFIFALSKIFVYYLVEQSAFVNYSTGTCWLATTTNHILAHLGIYNTFTAVLGNWPYVIGGGNALIALYSQFEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU26</accession>
    <name>G3AU26_SPAPN</name>
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        <recommendedName>
            <fullName evidence="3">Vps72/YL1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_157381</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
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                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS72/YL1 family.</text>
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        <property type="protein sequence ID" value="EGW30401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007377310.1"/>
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    <dbReference type="FunCoup" id="G3AU26">
        <property type="interactions" value="93"/>
    </dbReference>
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    <dbReference type="GeneID" id="18871078"/>
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    </dbReference>
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    <dbReference type="OMA" id="CQYFDPK"/>
    <dbReference type="OrthoDB" id="49520at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
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    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013272">
        <property type="entry name" value="Vps72/YL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046757">
        <property type="entry name" value="YL1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13275:SF4">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 72 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13275">
        <property type="entry name" value="YL-1 PROTEIN TRANSCRIPTION FACTOR-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05764">
        <property type="entry name" value="YL1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08265">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00993">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vps72/YL1 C-terminal" evidence="3">
        <location>
            <begin position="667"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="25"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="328"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="331"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="375"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="405"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="438"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="451"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW30401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="742" mass="85856" checksum="7E7004856B5E25D0" modified="2011-11-16" version="1">MDEESLIATRSRRANAGSRLKQLIEQEQQGLEDTSQFITEDDDNVNLLFQVEGEDEEFIEESEEEEEEEEEEEGDDGEEGKQQEEADEEEESGSRKRRHPEEDQDQDDVFSDSDLSESDSDQSEGEKELQRQERIKKRQLKKKRAIVPEIKTVKTQSPKKKPKRTPLITSDSLLMSQRRSSSRAAAVESKHALIEKLKESEERRAKYLSRTVHKKKSRELTQQERLDEALETEKANVESLMRFREQEIVKKEKQRQLLLSKRIKLQNIIRLVSKETFIYPIEEVKQARKEYERNVRGRRKLGKKKMAQLAEEGELLVLPPFEIDQDLPLVKQEREKREEEERIRKEQEEKEREEEEKRKALEGDTEEANNGQDVSSKEEPIPSEMDVDHPVESEVVDVDVKPEQSENEIDNMEENESVSTEIKVEEGQDDIIPEPISEQVQQEEFSPDTQNKAEEEINTEKDTIHEEEPTNEDQDKEKESPKPETDETIKDEATAEIKEEKRVKFADEIELEKEETPTPTPIALHTSNNETFEGPPQRVCRNTLYFLDFNEDKRELRLNFQNLKSILFGKQSLLPASRRFKEVKTILHIGKVEPYAHVKQESDDLFESVDKLTEENPIFDELKKLPRLGIKQDVVETIEEDVEQESAEINITTEAPTGIYLPNGNKKTCMISGTEVKYFDPSTGIPYSSVETYKLLKLIEQGQIPWLSLTPETNDTGIVEIYLGSRDGSTKHAAGVPEGFDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>G3AU87</accession>
    <name>G3AU87_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63293</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377434.1">
        <property type="nucleotide sequence ID" value="XM_007377372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AU87"/>
    <dbReference type="GeneID" id="18874686"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63293"/>
    <dbReference type="eggNOG" id="ENOG502QYDY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1619645_0_0_1"/>
    <dbReference type="InParanoid" id="G3AU87"/>
    <dbReference type="OMA" id="FTNEHKE"/>
    <dbReference type="OrthoDB" id="4026701at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="163" mass="17685" checksum="8DC8C978D0C47805" modified="2011-11-16" version="1">MALKDRVKRVSKIFTNEHKEALDKQTKDKEASAKDATEDAKDGAEEAAAEGETKAEEIAADGEAQVEEVADKVEGEAKEAQETAEEAPKEVEAEAEEVAAEGEEATKEVEGETKDAVDEAKTDAETAKAQVKDASEKVDKAHKKNDFFKKLFGKFKKTTPAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>G3AUD2</accession>
    <name>G3AUD2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Initiation-specific alpha-1,6-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">SPAPADRAFT_143242</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996505">
        <property type="protein sequence ID" value="EGW30508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377479.1">
        <property type="nucleotide sequence ID" value="XM_007377417.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AUD2"/>
    <dbReference type="FunCoup" id="G3AUD2">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AUD2"/>
    <dbReference type="GeneID" id="18870528"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_143242"/>
    <dbReference type="eggNOG" id="ENOG502QW2I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022381_5_0_1"/>
    <dbReference type="InParanoid" id="G3AUD2"/>
    <dbReference type="OMA" id="KEWIGWG"/>
    <dbReference type="OrthoDB" id="409543at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039367">
        <property type="entry name" value="Och1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834:SF1">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="376"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="377"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EGW30508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="57636" checksum="7CE91EDE75FEC05C" modified="2011-11-16" version="1">MIFNLRRPKVVFVLALFVAVSTTYFCLKSLNSGTPILSPLSSTKSYFDYQAPKEQFRLELEDHPDYKTTGLNFKPKYPSSLLYNTTVLEQLNYLFPYDASQPLPKQIWQMWKVGLDHEEFPDDFKKFHQTWVDNSPEYDHLLKTNAECDELVGELYGNVPDIIHAYNAMPYTILKCDFSRYLLLFAYGGIYTDIDTILLKPISEWISSESQYLNKSLDIGLIVGLESDRAYNWQRWAARRVQINSWTFMVKRGHPMLAELINKITEDTLWRESTGQLNATLGRDHGDNVNNWTGPGRFTDFVFKHMNNLLQTDFRKYDTLVKWPFLDKVELPIAVGDTMILPKKSMSPILPMNSMRAANNPDDDPLAYISHKFSGAWKKDQDRKMKEERKKEEQKKKEEEQKKKEEEERRKEEERKKEEEEEKKKEEERKKKEEEEKKKEEEKKKEEERKKKEEEEKKEEEKKKEEEKKKEEERKKEEEEKSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV48</accession>
    <name>G3AV48_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein RACK1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Guanine nucleotide-binding protein subunit beta-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_63737</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat G protein beta family. Ribosomal protein RACK1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377888.1">
        <property type="nucleotide sequence ID" value="XM_007377826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV48"/>
    <dbReference type="FunCoup" id="G3AV48">
        <property type="interactions" value="1402"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV48"/>
    <dbReference type="GeneID" id="18874890"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63737"/>
    <dbReference type="eggNOG" id="KOG0279">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_7_1"/>
    <dbReference type="InParanoid" id="G3AV48"/>
    <dbReference type="OMA" id="NCKLKIN"/>
    <dbReference type="OrthoDB" id="7875889at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001965">
        <property type="term" value="F:G-protein alpha-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030546">
        <property type="term" value="F:signaling receptor activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045182">
        <property type="term" value="F:translation regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140469">
        <property type="term" value="P:GCN2-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990145">
        <property type="term" value="P:maintenance of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061157">
        <property type="term" value="P:mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903138">
        <property type="term" value="P:negative regulation of cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902660">
        <property type="term" value="P:negative regulation of glucose mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903753">
        <property type="term" value="P:negative regulation of p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001125">
        <property type="term" value="P:negative regulation of translational frameshifting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031139">
        <property type="term" value="P:positive regulation of conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006521">
        <property type="term" value="P:regulation of amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000765">
        <property type="term" value="P:regulation of cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141014">
        <property type="term" value="P:ribosome hibernation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000039">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit beta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045223">
        <property type="entry name" value="RACK1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19868">
        <property type="entry name" value="RECEPTOR FOR ACTIVATED PROTEIN KINASE C RACK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="13"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="62"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="104"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="145"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="189"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="279"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW30122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="317" mass="34605" checksum="9E7C2F6E39D642AF" modified="2011-11-16" version="1">MADQEVLVLRGTLEGHNGWVTSLATTAAHPDLLLSGSRDKTLIRWKLTGGEDNQYGVPKKSFKGHSHIVQDVTISADGAYALSASWDRTLRLWDLETGETTERFVGHTGDVLSVSISKNLRQIVSASRDKTVKVWNTIGECMATLTGHKDWVSAVRISPADNSSTVISASWDKTVKSWDLTDYSVNADFIGHTGYISCITLSPDGSLCASAGKDGSINLWDLNSNRTLYTLDAKAEVHALAFSPNRYWLAAATNTGIKIFKLQERELLDELKPEFAIGANAKDPEAISLAWSADGQNLFAGYTDNVIRVWQVMTPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV74</accession>
    <name>G3AV74_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">SPAPADRAFT_63497</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377643.1">
        <property type="nucleotide sequence ID" value="XM_007377581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV74"/>
    <dbReference type="STRING" id="619300.G3AV74"/>
    <dbReference type="GeneID" id="18874782"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63497"/>
    <dbReference type="eggNOG" id="ENOG502SVID">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099559_0_0_1"/>
    <dbReference type="InParanoid" id="G3AV74"/>
    <dbReference type="OMA" id="NHCLIAN"/>
    <dbReference type="OrthoDB" id="4084730at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EGW29877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="244" mass="26923" checksum="BEF33A5F2DF5FF78" modified="2011-11-16" version="1">MFKSIFQSIKPMIINGATRSGVTVTATPATSITIYHNTNSLLSHNLLSKLSSYSVLPCTVHRFKDSHQTITSYTTDKVSSSNGQAPIAKFDVDVKFNQGLSKTDYDFIMNECVDIHPDNQSIMLQLLYGVSHDKRKLIKSFDLHDELYSNFDRVSQLSRENGPLIIDYNNKLIANDEASFDRIMVNYLSCGIQNVDKAGAGLGSNGSSNNSANLNVSAASTIPAAELYHQNLIHPHVAEFADLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AV77</accession>
    <name>G3AV77_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_63501</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377645.1">
        <property type="nucleotide sequence ID" value="XM_007377583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AV77"/>
    <dbReference type="FunCoup" id="G3AV77">
        <property type="interactions" value="1114"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AV77"/>
    <dbReference type="MEROPS" id="T01.P02"/>
    <dbReference type="GeneID" id="18874784"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63501"/>
    <dbReference type="eggNOG" id="KOG0180">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_10_0_1"/>
    <dbReference type="InParanoid" id="G3AV77"/>
    <dbReference type="OMA" id="CSEQLYG"/>
    <dbReference type="OrthoDB" id="204949at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03759">
        <property type="entry name" value="proteasome_beta_type_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000003">
        <property type="entry name" value="Proteasome subunit beta type-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033811">
        <property type="entry name" value="Proteasome_beta_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF10">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW29879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="206" mass="22662" checksum="F88E5FA7841FD04D" modified="2011-11-16" version="1">MSDPFSINGGSAVAMVGKDCIAIASDLRLGNQSLGVSNNFEKIFGFGDKCFLGLTGLATDVLTLKETFRFKTNLYKLREERSIEPETLANLVSSTLYERRFGPYFVSPIVAGLGHKDNKPFICGFDSIGCIDFAKDFIVSGTASDQLYGMCESLYEPDLEPEQLFETISQALLNAVDRDALSGWGAVVYIVTKDKVVKRVLKTRQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVD4</accession>
    <name>G3AVD4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">SPAPADRAFT_63761</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007377857.1"/>
    </dbReference>
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        <property type="interactions" value="146"/>
    </dbReference>
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    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63761"/>
    <dbReference type="eggNOG" id="KOG3968">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012358_0_2_1"/>
    <dbReference type="InParanoid" id="G3AVD4"/>
    <dbReference type="OMA" id="WHFRENA"/>
    <dbReference type="OrthoDB" id="194468at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008892">
        <property type="term" value="F:guanine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006147">
        <property type="term" value="P:guanine catabolic process"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
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        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
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        <property type="entry name" value="Metallo-dep_hydrolases"/>
    </dbReference>
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        <property type="entry name" value="GUANINE DEAMINASE"/>
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    </dbReference>
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        <property type="entry name" value="GUANINE DEAMINASE"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="5">
        <location>
            <begin position="30"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EGW30153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="312" mass="35420" checksum="BEC971F0223B303A" modified="2011-11-16" version="1">MDHNETYDKYQETYEECIHSMTKIITHLSNINPEEEVLVRPIVTPRFAPVCTRKLLKYLGNLSNINHLPIQTHISENKKEVELVHDLFPECPDYATVYDKHDLLNESTILAHAIHLTDKERKLIKKKNCSISHCPTSNTFISSGEAPIKQYLTKDKINVSLGTDISGGFDSSILGVVKHSILVSHHLAMKTNDTKDKLTLEDALYMSTMGGAKAVGLPDMIGSFELGKKFDVQLIDLTIEESNVDVFNWQIPDTSNTSESDYQHMVDLLGKWVFSGDDRNCVKVWCNGRLVLDKKVNGHRDDRWVMIESEFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVD6</accession>
    <name>G3AVD6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tag1-like fifth Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_63764</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW30155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_007377859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVD6"/>
    <dbReference type="GeneID" id="18874903"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_63764"/>
    <dbReference type="eggNOG" id="ENOG502QZVV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_794528_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVD6"/>
    <dbReference type="OMA" id="PHFIADA"/>
    <dbReference type="OrthoDB" id="5596576at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059066">
        <property type="entry name" value="Ig_Tag1-like_5th"/>
    </dbReference>
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        <property type="entry name" value="Tag1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35895">
        <property type="entry name" value="CHROMOSOME 16, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35895:SF3">
        <property type="entry name" value="PRE-RRNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26153">
        <property type="entry name" value="LEA-2L_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tag1-like fifth Ig-like" evidence="1">
        <location>
            <begin position="254"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW30155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="372" mass="41219" checksum="9C7DD4F18522C105" modified="2011-11-16" version="1">MQGTSNSHNQISLSEDCCHISTNTDSELTIIDSVSLGKLVRDVISSIEYDPVLLDVNLYDLVIETPILNMTVTNFTLASIPLLPGPNNLLLLTENQDSDFLNQLNATVYSVLYVGSSPTSLELLVDMSIFNPTNITFDLPNEVLELGVIYNNTNLAYGTIKDVFIPQNEQVNMSVGCTIKSETNEDKAVLEYFLSEFVSGGYENSSIKINIGNNHVYNNPGLNKLVEQINITNLTIPNISLPLPEESEQVAVKSCPFIVQATIHIFSSEVELTVFNPIVNHELIVEVFQAEAKYKDVVLGYLQQRERLIVPPGIYKTPKLPIKINSIGMDVLKKAMNGELDVEVLAILNVQLDEFDVNLFYKGNGLKSKIGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>G3AVU8</accession>
    <name>G3AVU8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_73424</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLA2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996506">
        <property type="protein sequence ID" value="EGW29993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007377759.1">
        <property type="nucleotide sequence ID" value="XM_007377697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AVU8"/>
    <dbReference type="FunCoup" id="G3AVU8">
        <property type="interactions" value="254"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AVU8"/>
    <dbReference type="GeneID" id="18875484"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_73424"/>
    <dbReference type="eggNOG" id="KOG0980">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004601_0_0_1"/>
    <dbReference type="InParanoid" id="G3AVU8"/>
    <dbReference type="OMA" id="FQMSVEM"/>
    <dbReference type="OrthoDB" id="10262320at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032051">
        <property type="term" value="F:clathrin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043325">
        <property type="term" value="F:phosphatidylinositol-3,4-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000147">
        <property type="term" value="P:actin cortical patch assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034316">
        <property type="term" value="P:negative regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17007">
        <property type="entry name" value="ANTH_N_Sla2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000012">
        <property type="entry name" value="Huntingtin interacting protein 1-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1410.10">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035964">
        <property type="entry name" value="I/LWEQ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002558">
        <property type="entry name" value="ILWEQ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030224">
        <property type="entry name" value="Sla2_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10407">
        <property type="entry name" value="HUNTINGTIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10407:SF15">
        <property type="entry name" value="HUNTINGTIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01608">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00307">
        <property type="entry name" value="ILWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109885">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50945">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="7">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="I/LWEQ" evidence="8">
        <location>
            <begin position="795"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="259"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="309"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="385"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1002"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EGW29993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="1040" mass="118033" checksum="6CAFBD9A0AEFE8C0" modified="2011-11-16" version="1">MSRAEVDLQTSVKKACNVDEVPPKRKHVRACIVYTWDHKNSRAFWNAVKIQPLQSNEVQLFKALIMIHKVLQEGHPNTLKDAYRNRDFISSLGSVFPTHGNAYGRLISQYDRYLLQKLDFHRNNPGFNGIFEYEEYISLRAVNDPNEGYESILQLMDLQDSINDLQKLIFATIHQTHNNLCKVSALVPLISESYGIYKFCTSMLRAMYQQLGADDALTVLVERFDSQHFMLRDFYTDCHSIKFLTSLITIPRLPNSAPNLQISDDGQAISRPRSANTTENSTPQLSAQPTSFEAPPNPPPEVDQQTGYLYQQQQEQARIQNELELQRQQQLQQQQEQQRLFEEQQREQERRFLQEQQALQMQQTQQQQSRVSELEHDLLMFKNQYDNDQSLLQQYDARVKTLENEMMTFNNTATQQIASKDEQIKNLEDQIDNWTKKYESLAKLYSQLRQEHLNLLAKFKKIQQKINSAQESILKKEKLEKDLKAKNVELADLIRERDRARLDIDRVKASKDQEIEKLEAEIRELTLSARESGKLQTLNLSSVMSKHQSELEDLKKQLADRELQLNDLGDSEALKDKLTEKELELEIAQESLDSALRELAKAKEDQDEIDNAQIDQILLSNIEKLKDLVDIFLDNNIRRILETKHELSSQAQAGNLNSTPEYLLSICEKTSDTANAFTSIFKNYVSEGKNSYYDDNSIYQDIILNSSELTVTINDLSMNAKGVAKSIPGAQEENLLTYVHGVLDSTLDYFQSIKSDQLNKLDDSGKAARVNDGNADLQNLLKELGSFVDGLKSHSGIKFSGNLEELVNDEMEQTAAIVSKASNFLNDLMANPSIHGGNLEVHEMILAAAKLVTDAVVNLISASVESQNEIVSKGKGTQTRTEFYKKNNRWTEGLISASKAVAGATNILISTADGVLRDVNSHEQLIVASNEVAASTAQLVAASRVKANFVSKTQDNLEHASSDVTSACKALVSKVQQLLTKEQDTNDIDLSKLTPYEGKTVEMEQQVEILKLENKLNAARKRLGEIRKHGYRDDESDDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADW4</accession>
    <name>G3ADW4_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial outer membrane protein OM14 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">SPAPADRAFT_57741</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372100.1">
        <property type="nucleotide sequence ID" value="XM_007372038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ADW4"/>
    <dbReference type="FunCoup" id="G3ADW4">
        <property type="interactions" value="105"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ADW4"/>
    <dbReference type="GeneID" id="18872177"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57741"/>
    <dbReference type="eggNOG" id="ENOG502S89U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127202_0_0_1"/>
    <dbReference type="InParanoid" id="G3ADW4"/>
    <dbReference type="OrthoDB" id="3980970at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990593">
        <property type="term" value="F:nascent polypeptide-associated complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039454">
        <property type="entry name" value="OM14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039453">
        <property type="entry name" value="OM14_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38402">
        <property type="entry name" value="MITOCHONDRIAL OUTER MEMBRANE PROTEIN OM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38402:SF1">
        <property type="entry name" value="MITOCHONDRIAL OUTER MEMBRANE PROTEIN OM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17304">
        <property type="entry name" value="OM14_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Mitochondrial outer membrane protein OM14 C-terminal" evidence="3">
        <location>
            <begin position="74"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="5"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EGW34688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="141" mass="15231" checksum="3933C9493B33C576" modified="2011-11-16" version="1">MSEHIPSAEEIQKEVEKQVDEVEKQVDEVTGNNDKECGIVSALKKAFNAVVDSVTKTANCAQTTACSTVSRVGVELQNPVVLAQVAVIASASAAGYFAHLEKYKIKSDNRAINCLNAAALTALVLADGFLFNQYYSKYDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>G3ADZ6</accession>
    <name>G3ADZ6_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein mS33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">SPAPADRAFT_57786</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS33 family.</text>
    </comment>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372132.1">
        <property type="nucleotide sequence ID" value="XM_007372070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3ADZ6"/>
    <dbReference type="FunCoup" id="G3ADZ6">
        <property type="interactions" value="209"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3ADZ6"/>
    <dbReference type="GeneID" id="18872196"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57786"/>
    <dbReference type="eggNOG" id="KOG4844">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150777_0_1_1"/>
    <dbReference type="InParanoid" id="G3ADZ6"/>
    <dbReference type="OMA" id="FKEWFPE"/>
    <dbReference type="OrthoDB" id="2257454at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013219">
        <property type="entry name" value="Ribosomal_mS33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13362">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13362:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08293">
        <property type="entry name" value="MRP-S33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EGW34720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="103" mass="11986" checksum="0A087D2C873ACE76" modified="2011-11-16" version="1">MSSILRKLPTKERLQQLKKLSSSIFSETYNPTCKRNGGQILKAELKGPEVLKYYGENSSMPTFKDFKNWFPELKLVDPRETHRLKYIAIRKLRNKGAPKKKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>G3AE53</accession>
    <name>G3AE53_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chitobiosyldiphosphodolichol beta-mannosyltransferase</fullName>
            <ecNumber evidence="3">2.4.1.142</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Asparagine-linked glycosylation protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">SPAPADRAFT_58808</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man(5) intermediate on the cytoplasmic surface of the ER.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.4.1.142" evidence="3"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372999.1">
        <property type="nucleotide sequence ID" value="XM_007372937.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AE53"/>
    <dbReference type="FunCoup" id="G3AE53">
        <property type="interactions" value="923"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AE53"/>
    <dbReference type="GeneID" id="18872618"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58808"/>
    <dbReference type="eggNOG" id="KOG2941">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012079_0_0_1"/>
    <dbReference type="InParanoid" id="G3AE53"/>
    <dbReference type="OMA" id="CKLIIDW"/>
    <dbReference type="OrthoDB" id="614844at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004578">
        <property type="term" value="F:chitobiosyldiphosphodolichol beta-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026051">
        <property type="entry name" value="ALG1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13036">
        <property type="entry name" value="BETA1,4 MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13036:SF0">
        <property type="entry name" value="CHITOBIOSYLDIPHOSPHODOLICHOL BETA-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13692">
        <property type="entry name" value="Glyco_trans_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EGW35587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="378" mass="42896" checksum="D967B4944C9BC017" modified="2011-11-16" version="1">MDYTVNLCGYLETEPPLKLIDDINIDINEIKAVKNTNGLPFILFAMLKVCVQVKQIFQLLFANGDSDFILIQNPPSIPLLAIIIVYIAIFNRNCKLIIDWHNLNYSILNLRYQNLNHPLVKLVKFYEQWLGKFAWLNITVTNQMSKFLVQEFGFPAQRITTLYDRPGDQFQPLAESKSDVMSRFDLFDKVQNIDSYKILVTSTSFTPDEDFEILLQALKQYHDTKNVPPVLLIVTGKGPLKQQFLERVIELDFSSKVIVKTAWLASEDYPTILSLADLGVSLHTSSSGIDLPMKIVDFFGCGIPVVSLSFPAINELVKEGVNGLLVTSDDKSSEMCKLFIKVFTSSELSAKLKLGALQESNLRWDQNWSTVLGKTFEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEE9</accession>
    <name>G3AEE9_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Gcn5-related n-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58940</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373149.1">
        <property type="nucleotide sequence ID" value="XM_007373087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEE9"/>
    <dbReference type="FunCoup" id="G3AEE9">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEE9"/>
    <dbReference type="GeneID" id="18872687"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58940"/>
    <dbReference type="eggNOG" id="ENOG502QPR6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059407_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEE9"/>
    <dbReference type="OMA" id="NGYARIM"/>
    <dbReference type="OrthoDB" id="2020070at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055100">
        <property type="entry name" value="GNAT_LYC1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053013">
        <property type="entry name" value="LAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34815">
        <property type="entry name" value="LYSINE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34815:SF2">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22998">
        <property type="entry name" value="GNAT_LYC1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="LYC1 C-terminal" evidence="1">
        <location>
            <begin position="182"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="411" mass="47649" checksum="200C5880FADC7F52" modified="2011-11-16" version="1">MSPSAVLNTNQYELVHLTDKEVIQFTRRQNSEAWKGRLSSEDYVLREHVLGKSKITSSDVNRLVVYMMRRVEDGTPLCSIELLIRKSWKFTFDETTNQVVQHDILSGCIGGVFTYPEHRKKGYARIMVDKLVKVAKSELLGEDGFTFLYSEVGEYYTKNGFLSLPVDLMNTPVDMNDDKLEQDPDVKDSQFELVDYHNFEPLMNDYITQFKQSIISKVQADHKTRVAVVPTSDIVDWFHLRSKYISYKLFHQDKAHNAIDFWNESYNSIRDKLVHNDPFKFGLKLYNADKSAVTAFIVWTMEWASTTENYASVLKTVVFEGDKDATTIKLLKLMKNYLIKNPIVDQTTSKIVIWESEVSSSVKEFLVNEWNTKHGIENSSRSAILINNQLEDEQLKSNELIWEGNDKIPWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEF8</accession>
    <name>G3AEF8_SPAPN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">SPAPADRAFT_58949</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW35745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007373157.1">
        <property type="nucleotide sequence ID" value="XM_007373095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEF8"/>
    <dbReference type="STRING" id="619300.G3AEF8"/>
    <dbReference type="GeneID" id="18872692"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_58949"/>
    <dbReference type="eggNOG" id="ENOG502RQ3M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126689_0_0_1"/>
    <dbReference type="InParanoid" id="G3AEF8"/>
    <dbReference type="OMA" id="DIGEQHY"/>
    <dbReference type="OrthoDB" id="3981234at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EGW35745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000709"/>
        </source>
    </evidence>
    <sequence length="217" mass="22005" checksum="2E0312A91565D533" modified="2011-11-16" version="1">MLPPPATTSTASYAYQPANVGANPGSTSQTPTANNTSRASGSTGTAGATNPSASNTPISGTGASSNPPPTNAQLTSKFNAHDIQVLRQLLVAGEKHKWKQITKEINQTSNNNAAAAAAAAATNSQQSGYGYRNANSGGAGGAAGTGANAGARAGGQSAPKNVSPTFVMKQYQSLLGLPNNSIYFGTLGSSLPYVVAPNGWDDIGEQHYQHHFGDDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-11-16" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>G3AEK2</accession>
    <name>G3AEK2_SPAPN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FK506-binding protein</fullName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">SPAPADRAFT_57832</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1)</name>
        <dbReference type="NCBI Taxonomy" id="619300"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Debaryomycetaceae</taxon>
            <taxon>Spathaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="13212" last="13217">
            <title>Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.</title>
            <authorList>
                <person name="Wohlbach D.J."/>
                <person name="Kuo A."/>
                <person name="Sato T.K."/>
                <person name="Potts K.M."/>
                <person name="Salamov A.A."/>
                <person name="LaButti K.M."/>
                <person name="Sun H."/>
                <person name="Clum A."/>
                <person name="Pangilinan J.L."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Jin M."/>
                <person name="Gunawan C."/>
                <person name="Balan V."/>
                <person name="Dale B.E."/>
                <person name="Jeffries T.W."/>
                <person name="Zinkel R."/>
                <person name="Barry K.W."/>
                <person name="Grigoriev I.V."/>
                <person name="Gasch A.P."/>
            </authorList>
            <dbReference type="PubMed" id="21788494"/>
            <dbReference type="DOI" id="10.1073/pnas.1103039108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">NRRL Y-27907 / 11-Y1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FKBP-type PPIase family. FKBP3/4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="GL996499">
        <property type="protein sequence ID" value="EGW34764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_007372176.1">
        <property type="nucleotide sequence ID" value="XM_007372114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G3AEK2"/>
    <dbReference type="FunCoup" id="G3AEK2">
        <property type="interactions" value="659"/>
    </dbReference>
    <dbReference type="STRING" id="619300.G3AEK2"/>
    <dbReference type="GeneID" id="18872212"/>
    <dbReference type="KEGG" id="spaa:SPAPADRAFT_57832"/>
    <dbReference type="eggNOG" id="KOG0552">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022297_3_1_1"/>
    <dbReference type="InParanoid" id="G3AEK2"/>
    <dbReference type="OMA" id="CPPHMAY"/>
    <dbReference type="OrthoDB" id="77911at2759"/>
    <dbReference type="Proteomes" id="UP000000709">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023566">
        <property type="entry name" value="PPIase_Fpr3/Fpr4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43811:SF19">
        <property type="entry name" value="39 KDA FK506-BINDING NUCLEAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43811">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
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    <dbReference type="PIRSF" id="PIRSF001473">
        <property type="entry name" value="FK506-bp_FPR3"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="8">
        <location>
            <begin position="329"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="66"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="164"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="202"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="231"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="251"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="273"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR001473"/>
        </source>
    </evidence>
    <evidence ty
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